Hemagglutination Tests
Hemagglutination
Hemagglutination Inhibition Tests
Hemagglutinins
Complement Fixation Tests
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Rubella virus
Erythrocytes
Influenza Vaccines
Vaccines used to prevent infection by viruses in the family ORTHOMYXOVIRIDAE. It includes both killed and attenuated vaccines. The composition of the vaccines is changed each year in response to antigenic shifts and changes in prevalence of influenza virus strains. The vaccine is usually bivalent or trivalent, containing one or two INFLUENZAVIRUS A strains and one INFLUENZAVIRUS B strain.
Immune Sera
Fimbriae, Bacterial
Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).
Neutralization Tests
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Rubella
Orthomyxoviridae
Cross Reactions
Immunodiffusion
Lectins
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Influenza A virus
The type species of the genus INFLUENZAVIRUS A that causes influenza and other diseases in humans and animals. Antigenic variation occurs frequently between strains, allowing classification into subtypes and variants. Transmission is usually by aerosol (human and most non-aquatic hosts) or waterborne (ducks). Infected birds shed the virus in their saliva, nasal secretions, and feces.
Influenza A Virus, H3N2 Subtype
Adhesins, Escherichia coli
Mannose
Influenza, Human
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Bacterial Adhesion
Immune Adherence Reaction
A method for the detection of very small quantities of antibody in which the antigen-antibody-complement complex adheres to indicator cells, usually primate erythrocytes or nonprimate blood platelets. The reaction is dependent on the number of bound C3 molecules on the C3b receptor sites of the indicator cell.
Influenza A Virus, H1N1 Subtype
Neuraminidase
Tannins
Polyphenolic compounds with molecular weights of around 500-3000 daltons and containing enough hydroxyl groups (1-2 per 100 MW) for effective cross linking of other compounds (ASTRINGENTS). The two main types are HYDROLYZABLE TANNINS and CONDENSED TANNINS. Historically, the term has applied to many compounds and plant extracts able to render skin COLLAGEN impervious to degradation. The word tannin derives from the Celtic word for OAK TREE which was used for leather processing.
Sheep
Agglutinins
Rabbits
Hemagglutinin Glycoproteins, Influenza Virus
Membrane glycoproteins from influenza viruses which are involved in hemagglutination, virus attachment, and envelope fusion. Fourteen distinct subtypes of HA glycoproteins and nine of NA glycoproteins have been identified from INFLUENZA A VIRUS; no subtypes have been identified for Influenza B or Influenza C viruses.
Evaluation Studies as Topic
Agglutination Tests
Immunoglobulin M
Vaccines, Inactivated
Chickens
Blood Group Antigens
Immunoglobulin G
Hemadsorption
Antibody Specificity
Antibody Formation
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Serotyping
Horses
Kaolin
The most common mineral of a group of hydrated aluminum silicates, approximately H2Al2Si2O8-H2O. It is prepared for pharmaceutical and medicinal purposes by levigating with water to remove sand, etc. (From Merck Index, 11th ed) The name is derived from Kao-ling (Chinese: "high ridge"), the original site. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Ether
Guinea Pigs
Mycoplasma
Counterimmunoelectrophoresis
Respirovirus
Latex Fixation Tests
Fimbriae Proteins
Hemolysis
Influenza A Virus, H9N2 Subtype
Influenza B virus
Species of the genus INFLUENZAVIRUS B that cause HUMAN INFLUENZA and other diseases primarily in humans. Antigenic variation is less extensive than in type A viruses (INFLUENZA A VIRUS) and consequently there is no basis for distinct subtypes or variants. Epidemics are less likely than with INFLUENZA A VIRUS and there have been no pandemics. Previously only found in humans, Influenza B virus has been isolated from seals which may constitute the animal reservoir from which humans are exposed.
Haplorhini
Antibodies
Adhesins, Bacterial
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
Carbohydrates
Plant Lectins
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Immunoenzyme Techniques
Yaws
Vaccination
Cattle
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Pasteurella
Influenza A Virus, H5N1 Subtype
A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 5 and neuraminidase 1. The H5N1 subtype, frequently referred to as the bird flu virus, is endemic in wild birds and very contagious among both domestic (POULTRY) and wild birds. It does not usually infect humans, but some cases have been reported.
Glutaral
Influenza A Virus, H3N8 Subtype
Seroepidemiologic Studies
Pyelonephritis
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Melioidosis
Arboviruses
Arthropod-borne viruses. A non-taxonomic designation for viruses that can replicate in both vertebrate hosts and arthropod vectors. Included are some members of the following families: ARENAVIRIDAE; BUNYAVIRIDAE; REOVIRIDAE; TOGAVIRIDAE; and FLAVIVIRIDAE. (From Dictionary of Microbiology and Molecular Biology, 2nd ed)
ABO Blood-Group System
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
Antigen-Antibody Reactions
Immunization
Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).
Polysorbates
Mumps virus
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Ovomucin
Influenza in Birds
Bunyaviridae
Rotavirus
Encephalitis Virus, Japanese
Immunoglobulin A
Madin Darby Canine Kidney Cells
Poultry Diseases
Paramyxoviridae
Newcastle disease virus
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Galactosides
Encephalitis, Arbovirus
Infections of the brain caused by arthropod-borne viruses (i.e., arboviruses) primarily from the families TOGAVIRIDAE; FLAVIVIRIDAE; BUNYAVIRIDAE; REOVIRIDAE; and RHABDOVIRIDAE. Life cycles of these viruses are characterized by ZOONOSES, with birds and lower mammals serving as intermediate hosts. The virus is transmitted to humans by the bite of mosquitoes (CULICIDAE) or TICKS. Clinical manifestations include fever, headache, alterations of mentation, focal neurologic deficits, and COMA. (From Clin Microbiol Rev 1994 Jan;7(1):89-116; Walton, Brain's Diseases of the Nervous System, 10th ed, p321)
Liver Abscess, Amebic
Sialic Acids
Reagins
Glycophorin
The major sialoglycoprotein of the human erythrocyte membrane. It consists of at least two sialoglycopeptides and is composed of 60% carbohydrate including sialic acid and 40% protein. It is involved in a number of different biological activities including the binding of MN blood groups, influenza viruses, kidney bean phytohemagglutinin, and wheat germ agglutinin.
Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
Newcastle Disease
Encephalitis Viruses
Reagent Kits, Diagnostic
Immunoelectrophoresis
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
False Positive Reactions
Parvoviridae
Parainfluenza Virus 1, Human
Bordetella avium
Urinary Tract Infections
Turkeys
Carbohydrate Sequence
Glycoconjugates
Cattle Diseases
Actinomyces
Encephalitis Virus, California
Porphyromonas gingivalis
Measles virus
Fusobacterium
Ferrets
Pandemics
N-Acetylneuraminic Acid
Amino Acid Sequence
Sensitivity and Specificity
Parainfluenza Virus 3, Human
Formaldehyde
A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)
P Blood-Group System
Dogs
Entamoeba histolytica
Mannosides
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
Coronavirus, Bovine
Chemical Precipitation
Viruses
Hepatovirus
Enterotoxins
Typhus, Epidemic Louse-Borne
The classic form of typhus, caused by RICKETTSIA PROWAZEKII, which is transmitted from man to man by the louse Pediculus humanus corporis. This disease is characterized by the sudden onset of intense headache, malaise, and generalized myalgia followed by the formation of a macular skin eruption and vascular and neurologic disturbances.
Fetuins
Chagas Disease
Infection with the protozoan parasite TRYPANOSOMA CRUZI, a form of TRYPANOSOMIASIS endemic in Central and South America. It is named after the Brazilian physician Carlos Chagas, who discovered the parasite. Infection by the parasite (positive serologic result only) is distinguished from the clinical manifestations that develop years later, such as destruction of PARASYMPATHETIC GANGLIA; CHAGAS CARDIOMYOPATHY; and dysfunction of the ESOPHAGUS or COLON.
MNSs Blood-Group System
Encephalomyelitis, Equine
A group of ALPHAVIRUS INFECTIONS which affect horses and man, transmitted via the bites of mosquitoes. Disorders in this category are endemic to regions of South America and North America. In humans, clinical manifestations vary with the type of infection, and range from a mild influenza-like syndrome to a fulminant encephalitis. (From Joynt, Clinical Neurology, 1996, Ch26, pp8-10)
Calcium Chloride
Hepatitis B Antigens
Encephalitis, California
A viral infection of the brain caused by serotypes of California encephalitis virus (ENCEPHALITIS VIRUS, CALIFORNIA) transmitted to humans by the mosquito AEDES triseriatus. The majority of cases are caused by the LA CROSSE VIRUS. This condition is endemic to the midwestern United States and primarily affects children between 5-10 years of age. Clinical manifestations include FEVER; VOMITING; HEADACHE; and abdominal pain followed by SEIZURES, altered mentation, and focal neurologic deficits. (From Joynt, Clinical Neurology, 1996, Ch26, p13)
Parvovirus, Canine
Feces
Hemolysin Proteins
Receptors, Virus
Glycoproteins
Virulence
Freeze Drying
Antitoxins
Galectins
A class of animal lectins that bind specifically to beta-galactoside in a calcium-independent manner. Members of this class are distiguished from other lectins by the presence of a conserved carbohydrate recognition domain. The majority of proteins in this class bind to sugar molecules in a sulfhydryl-dependent manner and are often referred to as S-type lectins, however this property is not required for membership in this class.
Injections, Intramuscular
Adjuvants, Immunologic
Substances that augment, stimulate, activate, potentiate, or modulate the immune response at either the cellular or humoral level. The classical agents (Freund's adjuvant, BCG, Corynebacterium parvum, et al.) contain bacterial antigens. Some are endogenous (e.g., histamine, interferon, transfer factor, tuftsin, interleukin-1). Their mode of action is either non-specific, resulting in increased immune responsiveness to a wide variety of antigens, or antigen-specific, i.e., affecting a restricted type of immune response to a narrow group of antigens. The therapeutic efficacy of many biological response modifiers is related to their antigen-specific immunoadjuvanticity.
Influenza A Virus, H7N7 Subtype
Measles
HN Protein
Temperature
Oligosaccharides
Galactose
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Mammalian orthoreovirus 3
Dysentery, Amebic
Glycolipids
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. (1/925)
The Vibrio cholerae genome contains a 5.4-kb pil gene cluster that resembles the Aeromonas hydrophila tap gene cluster and other type IV-A pilus assembly operons. The region consists of five complete open reading frames designated pilABCD and yacE, based on the nomenclature of related genes from Pseudomonas aeruginosa and Escherichia coli K-12. This cluster is present in both classical and El Tor biotypes, and the pilA and pilD genes are 100% conserved. The pilA gene encodes a putative type IV pilus subunit. However, deletion of pilA had no effect on either colonization of infant mice or adherence to HEp-2 cells, demonstrating that pilA does not encode the primary subunit of a pilus essential for these processes. The pilD gene product is similar to other type IV prepilin peptidases, proteins that process type IV signal sequences. Mutational analysis of the pilD gene showed that pilD is essential for secretion of cholera toxin and hemagglutinin-protease, mannose-sensitive hemagglutination (MSHA), production of toxin-coregulated pili, and colonization of infant mice. Defects in these functions are likely due to the lack of processing of N termini of four Eps secretion proteins, four proteins of the MSHA cluster, and TcpB, all of which contain type IV-A leader sequences. Some pilD mutants also showed reduced adherence to HEp-2 cells, but this defect could not be complemented in trans, indicating that the defect may not be directly due to a loss of pilD. Taken together, these data demonstrate the effectiveness of the V. cholerae genome project for rapid identification and characterization of potential virulence factors. (+info)Effect of temperature on growth, hemagglutination, and protease activity of Porphyromonas gingivalis. (2/925)
Bacteria persisting in periodontal pockets are exposed to elevated temperatures during periods of inflammation. Temperature is an environmental factor that can modulate gene expression. Consequently, in the present study we examined the effect of temperature on the expression of virulence determinants by the periodontopathogen, Porphyromonas gingivalis. P. gingivalis W50 was grown in a complex medium under hemin excess at pH 7.0 and at a constant temperature of either 37, 39, or 41 degrees C; cultures were monitored for protease and hemagglutinin activity. P. gingivalis grew well at all three temperatures. An increase in growth temperature from 37 to 39 degrees C resulted in a 65% reduction in both total arginine- and lysine-specific activities (P < 0.01). A further rise in growth temperature to 41 degrees C led to even greater reductions in arginine-specific (82%; P < 0.001) and lysine-specific (73%; P < 0. 01) activities. These reductions were also associated with an altered distribution of individual arginine-specific enzyme isoforms. At 41 degrees C, there was a disproportionate reduction in the level of the heterodimeric RI protease, which also contains adhesin domains. The reduction also correlated with a markedly diminished hemagglutination activity of cells, especially in those grown at 41 degrees C, and a reduced immunoreactivity with a monoclonal antibody which recognizes gene products involved in hemagglutination. Thus, as the environmental temperature increased, P. gingivalis adopted a less aggressive phenotype, while retaining cell population levels. The coordinate down-regulation of virulence gene expression in response to an environmental cue linked to the intensity of the host inflammatory response is consistent with the clinically observed cyclical nature of disease progression in periodontal diseases. (+info)Structural and functional studies of the measles virus hemagglutinin: identification of a novel site required for CD46 interaction. (3/925)
The entry of measles virus (MV) into human cells is mediated by the initial attachment of the viral hemagglutinin (HA) to the complement regulatory protein CD46. Two subdomains, one each within CD46 short consensus repeats (SCRs) 1 and 2, are responsible for this interaction. However, little is known about the regions within MV HA needed for a high-affinity CD46 interaction. To better define the HA-CD46 interaction, we took three approaches: chimeric domain swapping, peptide scanning, and alanine scanning mutagenesis. Chimeras of MV HA and the closely related rinderpest virus (RPV) HA were generated and tested for cell surface expression and the ability to hemadsorb CD46+ red blood cells (RBC). Exchanges with the N terminus of RPV were tolerated as MV HA could be replaced with RPV HA up to amino-acid position 154. However, both larger swaps with RPV and a small RPV HA replacement at the C terminus aborted cell-surface expression. Peptide scanning with 51 overlapping peptides derived from three MV HA regions showed one peptide, corresponding to MV HA amino acids 468-487, blocked hemagglutination of African green monkey (AGM) RBCs and inhibited MV infection of Chinese hamster ovary cells (CHO) expressing human CD46. Alanine scanning mutants mapped sites on the MV HA that were not required for trafficking to the cell surface or function in hemagglutination as well as a novel site required for CD46 interaction, amino acids 473-477. (+info)Characterization of nonenterotoxigenic Escherichia coli strains producing F17 fimbriae isolated from diarrheic lambs and goat kids. (4/925)
Forty-five ovine and caprine nonenterotoxigenic Escherichia coli strains producing F17-related fimbriae were characterized with respect to the fimbrial structural subunit and adhesin subtypes produced. In addition, several characteristics related to the virulence of strains producing F17 fimbriae were studied. Most of the strains (73%) possessed the f17cA structural subunit gene, whereas the f17aA and f17dA genes were detected only on three (6%) and two (4%) strains, respectively. The f17bA gene was not detected. All but one of these strains possessed the f17G genes of the adhesin subfamily II. The only strain having the f17G gene of subfamily I possessed the structural subunit gene f17dA. Sequencing of the f17A and f17G genes of four selected strains confirmed the association of f17cA and f17dA structural subunit genes with the f17G genes of the adhesin subfamily II. These results indicated that adhesins of the subfamily II are prominent among ovine and caprine isolates and that they are indistinctly associated with the F17 structural subunit subtypes on these field strains. CS31A- and CNF2-related genes were not detected. Most of the strains adhered in vitro to ovine intestinal brush borders (36 of 45) and agglutinated the erythrocytes of different species in the presence of D-mannose (39 of 45). F17-positive strains produced colicin V (57%) and were resistant to the bactericidal effect of serum (91%) in significantly higher percentages than F17-negative strains (34% produced colicin V, and 66% were serum resistant). Thus, most of the studied ovine and caprine strains showed phenotypic characteristics of septicemic strains. (+info)Characterization of the relationship between polysaccharide intercellular adhesin and hemagglutination in Staphylococcus epidermidis. (5/925)
To determine whether a relationship exists between biofilm formation and hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and hemagglutination. Strains that produced biofilm were significantly (P<.001) more likely to mediate hemagglutination (16 biofilm-positive/hemagglutination-positive strains and 19 biofilm-negative/hemagglutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated hemagglutination (>/=1/128). It was concluded that production of PIA and hemagglutination are strongly associated and that PIA, at least in part, mediates hemagglutination in S. epidermidis. (+info)Growth, cellular differentiation and virulence factor expression by Proteus mirabilis in vitro and in vivo. (6/925)
A uropathogenic strain of Proteus mirabilis was grown in vitro in human and mouse urine and brain-heart infusion broth (BHIB) and in vivo in subcutaneous open chambers (SOC) in mice, intraperitoneal diffusion chambers (IPC) in rats and by ascending urinary tract infection in mice in order to compare growth pattern, cellular differentiation and expression of virulence factors. Although the growth rate was slower in vivo than in vitro, the extent of growth was similar after 24 h. PR mirabilis differentiated into filamentous swarmer cells in all in-vitro culture conditions, but no filamentous cells were observed in either of the in-vivo chamber models. Transurethrally infected mice showed a rapid release or loss of filamentous cells and these could not be seen in kidney or bladder homogenates 7 days after infection. Bacteria showed increasing haemagglutination titres for fresh and tanned red blood cells after subculturing in BHIB, but bacteria grown in vivo did not show haemagglutination. An increasing resistance to normal serum was found when bacteria were grown in vivo. Significant haemolytic activity was detected with bacteria grown in BHIB and IPC, but almost no activity was found when bacteria had grown in urine. These findings improve the understanding of the role of P. mirabilis uropathogenic virulence factors in vivo. (+info)Normal human serum contains natural antibodies reactive with autologous ABO blood group antigens. (7/925)
It is widely accepted that the serum of healthy individuals contains natural antibodies only against those blood group A or B antigens that are not expressed on the individual's red blood cells. The mechanisms involved in tolerance to autologous blood group antigens remain unclear. In the present study, we show that IgM and IgG antibodies reactive with autologous blood group antigens are present in the immunoglobulin fraction of normal human serum. Natural IgG anti-A antibodies purified by affinity chromatography from IgG of individuals of blood group A exhibited an affinity for A trisaccharide antigen in the micromolar range and agglutinated A red cells at sixfold higher concentrations than those required for agglutination with affinity-purified anti-A IgG of individuals of blood group B. Whereas autoantibodies reactive with self A and B antigens are readily detected in purified IgG and IgM fractions, their expression is restricted in whole serum as a result of complementary interactions between variable regions of antibodies. These observations suggest that tolerance to autologous ABO blood group antigens is dependent on peripheral control of antibody autoreactivity. (+info)Genetic analyses of proteolysis, hemoglobin binding, and hemagglutination of Porphyromonas gingivalis. Construction of mutants with a combination of rgpA, rgpB, kgp, and hagA. (8/925)
Porphyromonas gingivalis produces arginine-specific cysteine proteinase (Arg-gingipain, RGP) and lysine-specific cysteine proteinase (Lys-gingipain, KGP) in the extracellular and cell-associated forms. Two separate genes (rgpA and rgpB) and a single gene (kgp) have been found to encode RGP and KGP, respectively. We constructed rgpA rgpB kgp triple mutants by homologous recombination with cloned rgp and kgp DNA interrupted by drug resistance gene markers. The triple mutants showed no RGP or KGP activity in either cell extracts or culture supernatants. The culture supernatants of the triple mutants grown in a rich medium had no proteolytic activity toward bovine serum albumin or gelatin derived from human type I collagen. Moreover, the mutants did not grow in a defined medium containing bovine serum albumin as the sole carbon/energy source. These results indicate that the proteolytic activity of P. gingivalis toward bovine serum albumin and gelatin derived from human type I collagen appears to be attributable to RGP and KGP. The hemagglutinin gene hagA of P. gingivalis possesses the adhesin domain regions responsible for hemagglutination and hemoglobin binding that are also located in the C-terminal regions of rgpA and kgp. A rgpA kgp hagA triple mutant constructed in this study exhibited no hemagglutination using sheep erythrocytes or hemoglobin binding activity, as determined by a solid-phase binding assay with horseradish peroxidase-conjugated human hemoglobin, indicating that the adhesin domains seem to be particularly important for P. gingivalis cells to agglutinate erythrocytes and bind hemoglobin, leading to heme acquisition. (+info)
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Influenza hemagglutination inhibition assay
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Hemagglutination
... , or haemagglutination, is a specific form of agglutination that involves red blood cells (RBCs). It has two ... Hemagglutination at the US National Library of Medicine Medical Subject Headings (MeSH) (All articles with unsourced statements ... the greatest dilution which inhibits hemagglutination). Muramatsu M, Gonzalez HD, Cacciola R, Aikawa A, Yaqoob MM, Puliatti C ( ... common uses in the laboratory: blood typing and the quantification of virus dilutions in a haemagglutination assay. Blood type ...
Hemagglutination assay
The hemagglutination assay or haemagglutination assay (HA) and the hemagglutination inhibition assay (HI or HAI) were developed ... HA and HI apply the process of hemagglutination, in which sialic acid receptors on the surface of red blood cells (RBCs) bind ... Hemagglutination is observed in the presence of staphylococci, vibrios, and other bacterial species, similar to the mechanism ... Hemagglutination Virus quantification Hirst, GK (1942). "The quantitative determination of Influenza virus and antibodies by ...
Haemagglutination activity domain
In molecular biology, the haemagglutination activity domain is a conserved protein domain found near the N terminus of a number ... This domain is suggested to be a carbohydrate-dependent haemagglutination activity site. In Bordetella pertussis, the ...
History and naming of human leukocyte antigens
Hemagglutination assay. In generating an immune response to an antigen, the B-cells go through a process of maturation, from ... The IgM can be used directly in hemagglutination assays, depicted on the right. IgM has 10 antigen binding regions per molecule ...
Laboratory diagnosis of viral infections
"Influenza hemagglutination inhibition assay". www.virology.ws. Retrieved 19 October 2020. (Articles with short description, ...
Virus quantification
The hemagglutination inhibition assay is a common variation of the HA assay used to measure flu-specific antibody levels in ... The hemagglutination assay (HA) is a common non-fluorescence protein quantification assay specific for influenza. It relies on ... "Influenza hemagglutination inhibition assay". "Pierce Protein Biology". Rodda, S.J.; Gallichio, H.A.; Hampson, A.W (1981). "The ... The assay produces a result of hemagglutination units (HAU), with typical pfu to HAU ratios in the 106 range. This assay takes ...
Murine respirovirus
... and hemagglutination assays, with particular emphasis on use of the ELISA for its high sensitivity (unlike the hemagglutination ... Killian ML (2008). "Hemagglutination assay for the avian influenza virus". Avian Influenza Virus. Methods in Molecular Biology ... Fukumi H, Nishikawa F, Kitayama T (August 1954). "A pneumotropic virus from mice causing hemagglutination". Japanese Journal of ... Hemagglutinin-neuraminidase (HN) is a single protein that induces hemagglutination and possesses neuraminidase (sialidase) ...
Agglutination (biology)
Hemagglutination is the process by which red blood cells agglutinate, meaning clump or clog. The agglutin involved in ... hemagglutination is called hemagglutinin. In cross-matching, donor red blood cells and the recipient's serum or plasma are ...
Pythiosis
Jindayok T, Piromsontikorn S, Srimuang S, Khupulsup K, Krajaejun T (July 2009). "Hemagglutination Test for Rapid Serodiagnosis ...
Galactose binding lectin domain
This lectin exists as a disulfide-linked homodimer of two subunits; the dimeric form is essential for hemagglutination activity ...
Avrainvillea
... erecta was reported to have strong hemagglutination activity. In addition, the chloroform fraction of a methanol ...
Hemagglutinin
On the contrary, if hemagglutination occurs, the test will result negative. Hemagglutination blood typing detection: this ... HIA (Hemagglutination Inhibition Assay): is a serologic assay which can be used either to screen for antibodies using RBCs with ... Cold agglutinin disease Hemagglutination assay Neuraminidase Influenza hemagglutinin (HA) Agglutination Bangaru, Sandhya; Lang ... "Hemagglutination detection for blood typing based on waveguide-mode sensors". Sensing and Bio-Sensing Research. 3: 59-64. doi: ...
Cold agglutinin disease
Cold hemagglutination was first reported by Landsteiner in 1903 and found to occur in human beings in 1918. The association of ... The auto antibodies responsible for hemagglutination at low temperatures, cold agglutinins (CA), may be found in the sera of ... RØRVIK, K (1954). "The syndrome of high-titre cold haemagglutination; a survey and a case report". Acta Medica Scandinavica. ... cold hemagglutination with hemolysis was described in 1937 by Rosenthal and Corten. During the 1960s, Dacie and Schubothe ...
Arbovirus
Mettler, N. E.; Clarke, D. H.; Casals, J. (1971). "Hemagglutination Inhibition with Arboviruses: Relationship Between Titers ... and hemagglutination-inhibition test. In the past, arboviruses were organized into one of four groups: A, B, C, and D. Group A ...
Virology
Older methods included complement fixation tests, hemagglutination inhibition and virus neutralisation. Newer methods us enzyme ...
Maria Zambon
Her main research areas include influenza vaccination and influenza hemagglutination inhibition. Her thesis on 1 - ...
Lectin
Many legume seeds have been proven to contain high lectin activity, termed hemagglutination. Soybean is the most important ... "Sialidase-Enhanced Lectin-Like Mechanism for Actinomyces viscosus and Actinomyces naeslundii Hemagglutination". Infection and ...
Human betaherpesvirus 5
Other tests include various fluorescence assays, indirect hemagglutination, (PCR) and latex agglutination. An ELISA technique ...
Kyasanur Forest disease
A seroprevalence study in Andaman and Nicobar islands in 2002 revealed a high prevalence of hemagglutination inhibition (HI) ... Other methods of diagnosis included hemagglutination inhibition (HI), complement fixation, neutralization tests. However, new ...
George C. Royal
Royal, Jr., George C.; Sampson, Calvin C.; Faggett, Timothy (1981). "A Rapid Passive Hemagglutination Method For Demonstrating ...
Macfarlane Burnet
... including hemagglutination assays. He worked on a live vaccine against influenza, but the vaccine was unsuccessful when tested ...
Chi-Ming Chu
Sampaio, A. A. C. (1952). "Inhibitors of influenza virus haemagglutination in normal animal sera". Bulletin of the World Health ...
Fujirebio
1966: Launch of HA Ag (TPHA), the world's first hemagglutination test for syphilis. 1978: Launch of the syphilis SERODIA®-TP•PA ...
Jerome T. Syverton
Brand, K. G.; Syverton, J. T. (1962). "Results of Species-Specific Hemagglutination Tests on "Transformed," Nontransformed, and ...
Lewis antigen system
Hutson, A. M.; Atmar, R. L.; Marcus, D. M.; Estes, M. K. (2003). "Norwalk virus-like particle hemagglutination by binding to h ...
Bartonella quintana
Cooper, M. D.; Hollingdale, M. R.; Vinson, J. W.; Costa, J. (1976). "A Passive Hemagglutination Test for Diagnosis of Trench ...
Titer
Many traditional serological tests such as hemagglutination or complement fixation employ this principle. Such tests can ...
Psittacine beak and feather disease
Khalesi, B.; Bonne, N.; Stewart, M.; Sharp, M.; Raidal, S.R. (2005). "A comparison of haemagglutination, haemagglutination ... and excreted antigen detection in feather dander using haemagglutination assay (HA) alongside serology using haemagglutination ... "Laboratory diagnosis of psittacine beak and feather disease by haemagglutination and haemagglutination inhibition". Australian ... Raidal, S.R.; Cross, M.G. (1994). "The haemagglutination spectrum of psittacine beak and feather disease virus". Avian ...
Adenia
They cause apoptosis, hemagglutination, inhibition of protein synthesis, and depurination of ribosomes and DNA. Mouse ...
Influenza D virus
Hemagglutination inhibition (HI) is one method of serology that detects antibodies for diagnostic purposes. Western blot ( ... No samples of influenza D virus were detected in serum samples from humans; however, hemagglutination-inhibiting antibodies ...
Browsing by Subject "Hemagglutination"
NHANES 2010-2010: Hemagglutination inhibition antibody titers to influenza A/ H1N1 viruses (Surplus) Data Documentation,...
Hemagglutination inhibition antibody titers to influenza A/ H1N1 viruses (Surplus) (SSHN10_R) RDC Only Data File: SSHN10_R.xpt ... SSH1N1CA: Geometric mean hemagglutination inhibition (HI) titer against the influenza A/H1N1 virus A/California/7/2009. ... SSH3N2PR: Geometric mean hemagglutination inhibition (HI) titer against the influenza AH3N2 virus A/Perth16/2009 Continuous ... SSH3N2BR: Geometric mean hemagglutination inhibition (HI) titer against the influenza A/H3N2 virus B/Brisbane/60/2008. ...
Searo-Diagnosis of Dengue Infections by Haemagglutination Inhibition Test (HI) in Suspected Cases in Chittagong, Bangladesh.
Diagnosis of human and animal brucellosis by the indirect haemagglutination test
Malarial Antibodies Measured by the Indirect Hemagglutination Test in West African Children in: The American Journal of...
Malarial Antibodies Measured by the Indirect Hemagglutination Test in West African Children published on Mar 1976 by The ... The indirect hemagglutination test with Plasmodium falciparum antigen was used to measure malarial antibodies in filter paper ... Malarial Antibodies Measured by the Indirect Hemagglutination Test in West African Children ...
Hemagglutination with simian papovavirus SA12<...
Shah, K. V. ; Valis, J. D. ; Daniel, R. W. / Hemagglutination with simian papovavirus SA12. In: Journal of clinical ... Shah, KV, Valis, JD & Daniel, RW 1978, Hemagglutination with simian papovavirus SA12, Journal of clinical microbiology, vol. ... Hemagglutination with simian papovavirus SA12. Journal of clinical microbiology. 1978 Dec 1;7(4):396-398. ... Shah, K. V., Valis, J. D., & Daniel, R. W. (1978). Hemagglutination with simian papovavirus SA12. Journal of clinical ...
Cigarette smoking and hemagglutination inhibition response to influenza after natural disease and immunization. | [email protected]
Equine influenza: evaluation of the humoral immune response through the hemagglutination inhibition and single radial...
From 4 equine groups, the antibody protection levels against influenza were evaluted through the hemagglutination inhibition ... 1996). Equine influenza: evaluation of the humoral immune response through the hemagglutination inhibition and single radial ... Equine influenza: evaluation of the humoral immune response through the hemagglutination inhibition and single radial ... Equine Influenza: evaluation of the humoral immune response through the hemagglutination inhibition and single radial ...
Comparison of complement dependent lytic, hemagglutination inhibition and microneutralization antibody responses in influenza...
Co MD, Cruz J, Takeda A, Ennis FA, Terajima M. Comparison of complement dependent lytic, hemagglutination inhibition and ... Comparison of complement dependent lytic, hemagglutination inhibition and microneutralization antibody responses in influenza ... Comparison of complement dependent lytic, hemagglutination inhibition and microneutralization antibody responses in influenza ...
Evaluation of the automated micro-hemagglutination assay for antibodies to Treponema pallidum.
Hemagglutination Tests Humans Research Article Syphilis Serodiagnosis Treponema Immobilization Test Treponema Pallidum ... Evaluation of the automated micro-hemagglutination assay for antibodies to Treponema pallidum.. ... Title : Evaluation of the automated micro-hemagglutination assay for antibodies to Treponema pallidum. Personal Author(s) : ... Evaluation of the automated micro-hemagglutination assay for antibodies to Treponema pallidum. ...
Toxoplasmosis Workup: Approach Considerations, Immunoglobulin Testing, Imaging Studies
Kinetics of Hemagglutination-Inhibiting Antibodies Following Maternal Influenza Vaccination Among Mothers With and Those...
Hemagglutination
... :. type of agglutination reaction is routinely performed to type red blood cells (RBCs), wherein RBCs are mixed ... Hemagglutination, immunology, Kuby Immunology - 5thEdition, microbial lexicon, Types of agglutination reactionsLeave a comment ... Posts about Hemagglutination written by MicroScopia IWM ...
Hemagglutination | Profiles RNS
"Hemagglutination" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Hemagglutination" by people in this website by year, and ... Rapid phenotyping of HPA-1a using either diabody-based hemagglutination or recombinant IgG1-based assays. Transfusion. 1999 Jul ... The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL). ...
Hemagglutination Inhibition Test
Principle of Hemagglutination Inhibition Test. HAI Titer: The maximum dilution of serum (Ab) that inhibits hemagglutination is ... Principle of Hemagglutination Inhibition Test. *When measles viruses and red blood cells come together, hemagglutination occurs ... Hemagglutination can detect the presence of the virus in infected cell cultures; particular suppression of hemagglutination can ... Hemagglutination is the process by which viral hemagglutinins react with red blood cells to form a lattice of agglutinated ...
IMSEAR at SEARO: Purification of toxoplasma haemagglutination antigen.
Enhanced isolation of influenza viruses in qualified cells improves the probability of well-matched vaccines | npj Vaccines
... hemagglutination differences alter hemagglutination inhibition responses against influenza A/H1N1. Viral. Immunol. 27, 174-178 ... The impact of egg adaptation/s in the HA protein on the antigenicity of A(H3N2) viruses was assessed by the haemagglutination ... Haemagglutination inhibition assay. Pre- and post-vaccination sera were used to analyse antibody responses. Subjects were ... Amniotic or allantoic fluid was harvested and the presence of virus was assessed by Haemagglutination (HA) Assay using 1% ...
Latex- & Haemagglutination .::. DIALAB GmbH
E.S. Kilelu et al (1988). Diagnosis of Nairobi. Sheep Disease virus using Passive Haemagglutination and Haemagglutination...
A rapid antibody screening haemagglutination test for predicting immunity to SARS-CoV-2 variants of concern. - Nuffield...
Methods: Firstly, we developed a receptor-binding domain-specific haemagglutination test (HAT) to Wuhan and VOC (alpha, beta, ... A rapid antibody screening haemagglutination test for predicting immunity to SARS-CoV-2 variants of concern. Share Share Share ... Methods: Firstly, we developed a receptor-binding domain-specific haemagglutination test (HAT) to Wuhan and VOC (alpha, beta, ... A rapid antibody screening haemagglutination test for predicting immunity to SARS-CoV-2 variants of concern. ...
Assignment: Haemagglutination in RBC - NursingPaperAcers
Haemagglutination in RBC- when people sneeze, cough or speak - Indirect - Up to 1 metre• Inhaling droplets through direct ... Assignment: Haemagglutination in RBC. Assignment: Haemagglutination in RBC. ORDER NOW FOR AN ORIGINAL PAPER:Assignment: ... Haemagglutinin • HA named because it causes haemagglutination in RBC. • Binds to sialic acid on epithelial cells to initiated ...
tanned red cell hemagglutination inhibition immunoassay | Taber's Medical Dictionary
tanned red cell hemagglutination inhibition immunoassay answers are found in the Tabers Medical Dictionary powered by Unbound ... Tanned red cell hemagglutination inhibition immunoassay. In: Venes DD, ed. Tabers Medical Dictionary. F.A. Davis Company; 2021 ... Tanned red cell hemagglutination inhibition immunoassay. (2021). In Venes, D. (Ed.), Tabers Medical Dictionary (24th ed.). F.A ... "Tanned Red Cell Hemagglutination Inhibition Immunoassay." Tabers Medical Dictionary, 24th ed., F.A. Davis Company, 2021. ...
ArboCat Virus: Almpiwar (ALMV)
ArboCat Virus: Eastern equine encephalomyelitis (EEEV)
ArboCat Virus: Chenuda (CNUV)
Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC | Virology Journal | Full Text
Table - Acanthamoeba polyphaga mimivirus Virophage Seroconversion in Travelers Returning from Laos - Volume 18, Number 9...
Haemagglutination and cytotoxic studies of H-2. V. T-e anti-27, 28, 29 family of antibodies. - Wikidata
AssayAntibodiesAssaysTiterTitersViralEnzyme immunoassaysAntigenMeSHInfectionsELISAImmunityTestVirusesGlycanDiseaseActivityProtectionComparisonCellDataSiteTopicAssaysTreponemaHemolysisAntibody titersSerumAgglutinationInhibition of hemagglutinationVaccineTestsAdenovirusesTitresPassiveErythrocytesInhibitorInteractionSensitivityDoseInteractionsBloodActivity
Assay8
- This data release contains the hemagglutination inhibition assay titers to 2009 H1N1 and three contemporary influenza viruses. (cdc.gov)
- Antibody titers against 2009 H1N1 and three contemporary influenza viruses were measured with the hemagglutination inhibition (HI) assay following standard procedures by Focus Diagnositcs, Inc., Cypress, CA. (cdc.gov)
- Evaluation of the automated micro-hemagglutination assay for antibodies to Treponema pallidum. (cdc.gov)
- 1:10 by hemagglutination inhibition assay). (cmaj.ca)
- and B Malaysia) were determined by the Hemagglutination inhibition assay method. (clinicaltrials.gov)
- Thus, we performed crystallization and structure determination assays of mGal-1, and determined the carbohydrate binding specificy of mGal-1 and hGal-1 using a glycan array and using hemagglutination assay. (usp.br)
- Selected piglets from the litters were sampled multiple days post vaccination (DPV) by collecting nasal swabs and blood, and were tested using a LAIV vaccine specific RT-PCR assay and hemagglutination inhibition assay against the LAIV strains respectively. (umnswinenews.com)
- Although repeated exposure to influenza H3 induced the so-called "original antigenic sin" phenomena in hemagglutination inhibition assay titers against exposure to viruses later on, the ferret antibodies showed gradually enhanced avidity for different H3/hemagglutinin. (infectiousdiseaseadvisor.com)
Antibodies8
- The indirect hemagglutination test with Plasmodium falciparum antigen was used to measure malarial antibodies in filter paper blood specimens from 527 West African children. (ajtmh.org)
- Kinetics of Hemagglutination-Inhibiting Antibodies Following Maternal Influenza Vaccination Among Mothers With and Those Without HIV Infection and Their Infants. (ucdenver.edu)
- If the patient's serum lacks antibodies against the surface proteins of the test virus, hemagglutination will occur because surface molecules are free to hemagglutinate red blood cells (negative result). (microbiologynote.com)
- Therefore, hemagglutination is suppressed in the presence of antibodies. (microbiologynote.com)
- If the serum contains no antibodies that react with the measles virus, then all wells will display hemagglutination. (microbiologynote.com)
- Similarly, if measles virus-specific antibodies are present, hemagglutination will not occur until the antibodies are sufficiently diluted. (microbiologynote.com)
- Background: The association of hemagglutination inhibition (HAI) antibodies with protection from influenza among healthcare personnel (HCP) with occupational exposure to influenza viruses has not been well-described. (cdc.gov)
- Hemagglutination-inhibition test was performed to detect specific antibodies against Omsk hemorrhagic fever virus. (dtic.mil)
Assays5
- Rapid phenotyping of HPA-1a using either diabody-based hemagglutination or recombinant IgG1-based assays. (ouhsc.edu)
- Methods: Firstly, we developed a receptor-binding domain-specific haemagglutination test (HAT) to Wuhan and VOC (alpha, beta, gamma and delta) and compared to pseudotype, microneutralisation and virus neutralisation assays in 835 convalescent sera. (ox.ac.uk)
- Diverse modifications to the biaryl ring to improve druglike physical and pharmacokinetic properties of mannosides were assessed for FimH binding affinity based on their effects on hemagglutination and biofilm formation along with direct FimH binding assays. (nih.gov)
- This study was conducted to isolate and identify the EDS virus in the chicken layer that was diagnosed as a disease of EDS by hemagglutination (HA) and hemagglutination inhibition (HI) assays. (ugm.ac.id)
- Serologic tests include agglutination tests, indirect hemagglutination, complement fixation, immunofluorescence assays and enzyme immunoassays. (nj.gov)
Titer1
- The maximum dilution of serum (Ab) that inhibits hemagglutination is known as the serum's HAI titer. (microbiologynote.com)
Titers1
- Geometric Mean Titers (GMTs) of Hemagglutination Inhibition Antibody Titers Pre- and Post-vaccination With Fluzone® High Dose or Standard Fluzone® Vaccines. (clinicaltrials.gov)
Viral2
- Hemagglutination is the process by which viral hemagglutinins react with red blood cells to form a lattice of agglutinated cells that settle unevenly in a tube or microtiter well. (microbiologynote.com)
- The presence of non-specific inhibitors of viral haemagglutination and naturally occurring agglutinins of the erythrocytes may complicate the HAI test. (microbiologynote.com)
Enzyme immunoassays1
- Despite the wide distribution of SINV, symptomatic and enzyme immunoassays and hemagglutination-inhibition infections in humans have been reported in only a few for the human samples. (cdc.gov)
Antigen3
- IMSEAR at SEARO: Purification of toxoplasma haemagglutination antigen. (who.int)
- Kumar PS, Kumar R, Mohapatra LN. Purification of toxoplasma haemagglutination antigen. (who.int)
- It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. (biolegend.com)
MeSH1
- Hemagglutination" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
Infections1
- Searo-Diagnosis of Dengue Infections by Haemagglutination Inhibition Test (HI) in Suspected Cases in Chittagong, Bangladesh. (who.int)
ELISA1
- Piglet sera were tested by ELISA and hemagglutination inhibition. (umnswinenews.com)
Immunity2
- A rapid antibody screening haemagglutination test for predicting immunity to SARS-CoV-2 variants of concern. (ox.ac.uk)
- Impact of hemagglutination activity and M2e immunity on conferring protection against influenza viruses. (amedeo.com)
Test1
- Sheep Disease virus using Passive Haemagglutination and Haemagglutination inhibition test. (ac.ke)
Viruses2
- When measles viruses and red blood cells come together, hemagglutination occurs (see image a). (microbiologynote.com)
- Back titration of the antigen's hemagglutination activity (to verify that four hemagglutinating viruses (HAU) were examined). (microbiologynote.com)
Glycan1
- Consequently, rLSL-N exhibits a weak hemagglutination activity to a rabbit reticulocyte through the glycan binding to subsites of the lectin. (tcichemicals.com)
Disease1
- Cigarette smoking and hemagglutination inhibition response to influenza after natural disease and immunization. (duke.edu)
Activity1
- Crystal Structure of A Plant Albumin From Cicer Arietinum (Chickpea) Possessing Hemopexin Fold and Hemagglutination Activity Planta 2015 . (atomistry.com)
Protection1
- From 4 equine groups, the antibody protection levels against influenza were evaluted through the hemagglutination inhibition and single radial haemolysis. (usp.br)
Comparison2
- Comparison of complement dependent lytic, hemagglutination inhibition and microneutralization antibody responses in influenza vaccinated individuals. (umassmed.edu)
- Co MD, Cruz J, Takeda A, Ennis FA, Terajima M. Comparison of complement dependent lytic, hemagglutination inhibition and microneutralization antibody responses in influenza vaccinated individuals. (umassmed.edu)
Cell2
- tanned red cell hemagglutination inhibition immunoassay is a topic covered in the Taber's Medical Dictionary . (unboundmedicine.com)
- Nursing Central Redesign , nursing.unboundmedicine.com/nursingcentral/view/Tabers-Dictionary/761102/all/tanned_red_cell_hemagglutination_inhibition_immunoassay. (unboundmedicine.com)
Data1
- Hemagglutination inhibition data was used to visualize the antigenic relatedness of the isolates. (usda.gov)
Site1
- Sequencing of the haemagglutination gene (HA) in nine representative H5N1 isolates revealed a multi-basic amino acid motif at the cleavage site (321-PQGEKRRKKR/GLF-333), which is characteristic of highly pathogenic AIV, in five of our isolates, while the other four isolates showed an amino acid substitution (Q322K) at this cleavage site to make it (321-P K GEKRRKKR/GLF-333). (peerj.com)
Topic1
- This graph shows the total number of publications written about "Hemagglutination" by people in this website by year, and whether "Hemagglutination" was a major or minor topic of these publications. (ouhsc.edu)
Assays8
- CypherOne reads, analyzes, and creates a digital record of hemagglutination and hemagglutination inhibition assays. (gatewaybiosciences.com)
- Hemagglutination inhibition (HI) and microneutralization (MN) assays were performed following standard procedures * using A/Minnesota/11/2010 (H3N2)v and seasonal influenza viruses, A/Wisconsin/67/2005 (H3N2), and A/Perth/16/2009 (H3N2). (cdc.gov)
- Methods: Firstly, we developed a receptor-binding domain-specific haemagglutination test (HAT) to Wuhan and VOC (alpha, beta, gamma and delta) and compared to pseudotype, microneutralisation and virus neutralisation assays in 835 convalescent sera. (gob.pe)
- With the expanded number of longitudinal studies examining natural influenza infections in different population groups, as well as the expanded number of clinical trials investigating the efficacy of next-generation influenza vaccines, the development of improved immunological assays to measure correlates other than hemagglutination inhibition (HAI), microneutralization (MN) or neuraminidase inhibition (NAI) is timely and needed. (nih.gov)
- This is the hemagglutination inhibition (HI) or neutralization buy cosopt eye drops assays. (communigator.co.nz)
- Laboratory-based methods assessing antibody function (e.g. haemagglutination inhibition assays, serum neutralisation assays) are considered the gold standard. (ava.com.au)
- The mAbs were characterized in ELISA, hemagglutination inhibition, western and neutralization assays. (nih.gov)
- These proteins, which can be detected through hemagglutination assays, interact with different carbohydrates present in cell and viral surfaces. (getnews.info)
Treponema2
- What are the benefits of TREPONEMA PALLIDUM HAEMAGGLUTINATION Full Body Checkup? (hellodox.com)
- They found a positive Treponema pallidum haemagglutination test in 6.4% of girls and 1.1% of boys. (bmj.com)
Hemolysis4
- Ectopic pth secretion may due to hemagglutination and hemolysis. (aaan.org)
- Glycophorins A carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. (beckman.com)
- By 7 d post vaccination, the vaccine fulfilled the licensing criteria for antibody responses to the HA detected by haemagglutination inhibition and single radial hemolysis. (scoop.it)
- A positive result may be in the form of agglutinating or lysing red cells referred to as hemagglutination and/or hemolysis, respectively. (vin.com)
Antibody titers2
- Hemagglutination inhibition antibody titers were measured against influenza A and B strains included in the formulation of the vaccines and against mismatched strains. (lww.com)
- Both, the adenoviral vector AdHACD and the recombinant protein rHACD, induced hemagglutination-inhibiting antibody titers significantly higher than their counterparts carrying HA alone. (sld.cu)
Serum1
- Serum hemagglutination inhibition titers were measured on days 0 (prevaccination), 8, 21, 29 and 42. (elsevier.com)
Agglutination2
- Whereas with viral hemagglutination there is a clumping ( agglutination ) of red blood cells ( hemagglutination ), and this occurs specifically as a consequence of binding of individual virions to more than one red blood cell, with viral hemagglutination inhibition that virus- cell interaction is blocked by antibody binding. (biologyaspoetry.com)
- Rapoza classified monkey adenoviruses into 4 hemagglutination subgroups on the basis of their differential agglutination properties with respect to their ability to agglutinate rat, rhesus, and guinea pig erythrocytes ( 6 ). (cdc.gov)
Inhibition of hemagglutination1
- Specificity mapping of bacterial lectins by inhibition of hemagglutination using deoxy and deoxyfluoro analogs of receptor-active saccharides. (wustl.edu)
Vaccine1
- All participants had hemagglutination-inhibition (HAI) titers measured to all four vaccine- specific influenza antigens (A/H1N1, A/H3N2, B/Victoria, and B/Yamagata) before each vaccine dose and 28 to 42 days after the second dose. (medscape.com)
Tests4
- Hemagglutination tests were used at doctor's offices to diagnose pregnancy in the 1960s and 1970s. (nih.gov)
- Hemagglutination inhibition and neutralization tests indicated that 8KS0813 was antigenically related to a prototype strain, APMV-6/duck/Hong Kong/18/199/77, but with an 8- and 16-fold difference, respectively, in their titers. (nih.gov)
- Tests of Hemagglutination, Hemagglutination Inhibition, Complement Fixation, Neutralization in suckling mice and sensitivity to Sodium Deoxycholate were employed for identification of the isolate. (eurekamag.com)
- According to tests of Complement Fixation, Hemagglutination Inhibition and Neutralization in mice, the virus was identified as the same as Iguape virus. (eurekamag.com)
Adenoviruses2
- Hemagglutination by adenoviruses. (nih.gov)
- Since hemagglutination (HA) by human adenoviruses was first demonstrated by Rosén in 1958 (34), it has been shown that members of the six subgenera (A to F) display different HA properties (2, 26). (moviecultists.com)
Titres2
- Prevalence of pandemic (H1N1) 2009 haemagglutination inhibition (HAI) antibody titres ≥ 1:40 (putative protective level) in pre-pandemic sera. (mja.com.au)
- Few children but a greater proportion of adults showed pre-immunisation haemagglutination inhibition titres ≥40 to either virus. (eurosurveillance.org)
Passive1
- HBsAg and anti-HBs were tested by reversed passive hemagglutination and passive hemagglutination, respectively. (e-epih.org)
Erythrocytes1
- Lipids extracted from both inagglutinable and agglutinable erythrocytes inhibited arbovirus haemagglutination. (microbiologyresearch.org)
Inhibitor1
- Mode of action of an inhibitor from agar on growth and hemagglutination of group A arboviruses. (microbiologyresearch.org)
Interaction1
- Results SRF231 binds CD47 and disrupts the CD47/SIRPα interaction without causing hemagglutination or RBC phagocytosis. (bmj.com)
Sensitivity1
- Further laboratory investigation is required with indirect hemagglutination (sensitivity of 85%), which remains positive for several years, or immunoelectrophoresis (sensitivity of 95%), which remains positive for only 1 year after eradication. (medscape.com)
Dose1
- The 15 μg dosage elicited a seroprotective hemagglutination inhibition (≥1:40) in 20%, 47% and 93% of children in the 6-35 month, 3-9 year and 10-17 year age strata 21 days after dose 1 and in 78%, 82% and 98% of children 21 days after dose 2, respectively. (elsevier.com)
Interactions1
- The protein was found to be an endogenous platelet participant in the haemagglutination process through its interactions with platelet bound fibrinogen. (bmj.com)
Blood1
- Use these 96-well microplates for micro-volume applications such as storage, sample or reagent transfer, serial dilution, hemagglutination, blood typing and compliment fixation. (fishersci.com)
Activity3
- BFDV VLPs also possessed haemagglutinating activity which provides further evidence that self-assembled BFDV VLPs retain receptor mediated biological activity and that the determinants for BFDV haemagglutination activity rely solely on the capsid protein. (edu.au)
- E. ochreata treatment showed increased haemagglutination titre compared to negative control, thereby providing evidence that D. bulbifera and E. ochreata possessed immunomodulatory activity. (ijpsonline.com)
- 65 kDa) that causes hemagglutination and has acetylesterase activity. (mhmedical.com)