The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A species of ENTEROVIRUS which is the causal agent of POLIOMYELITIS in humans. Three serotypes (strains) exist. Transmission is by the fecal-oral route, pharyngeal secretions, or mechanical vector (flies). Vaccines with both inactivated and live attenuated virus have proven effective in immunizing against the infection.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Established cell cultures that have the potential to propagate indefinitely.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The rate dynamics in chemical or physical systems.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The sum of the weight of all the atoms in a molecule.
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Preparations of cell constituents or subcellular materials, isolates, or substances.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Proteins found in any species of virus.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The process by which a DNA molecule is duplicated.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Proteins prepared by recombinant DNA technology.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A genus of PICORNAVIRIDAE inhabiting primarily the respiratory tract of mammalian hosts. It includes over 100 human serotypes associated with the COMMON COLD.
Transport proteins that carry specific substances in the blood or across cell membranes.
The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Nuclear nonribosomal RNA larger than about 1000 nucleotides, the mass of which is rapidly synthesized and degraded within the cell nucleus. Some heterogeneous nuclear RNA may be a precursor to mRNA. However, the great bulk of total hnRNA hybridizes with nuclear DNA rather than with mRNA.
The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).
Ribonucleic acid that makes up the genetic material of viruses.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Highly conserved nuclear RNA-protein complexes that function in RNA processing in the nucleus, including pre-mRNA splicing and pre-mRNA 3'-end processing in the nucleoplasm, and pre-rRNA processing in the nucleolus (see RIBONUCLEOPROTEINS, SMALL NUCLEOLAR).
A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.
A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
Proteins that specifically bind to RNA CAPS and form nuclear cap binding protein complexes. In addition to stabilizing the 5' end of mRNAs, they serve a diverse array of functions such as enhancing mRNA transport out of the CELL NUCLEUS and regulating MRNA TRANSLATION in the CYTOPLASM.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
A bacterium which is one of the etiologic agents of bacillary dysentery (DYSENTERY, BACILLARY) and sometimes of infantile gastroenteritis.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Elements of limited time intervals, contributing to particular results or situations.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Short chains of RNA (100-300 nucleotides long) that are abundant in the nucleus and usually complexed with proteins in snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR). Many function in the processing of messenger RNA precursors. Others, the snoRNAs (RNA, SMALL NUCLEOLAR), are involved with the processing of ribosomal RNA precursors.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Nucleic acid sequences involved in regulating the expression of genes.

The incorporation of 5-iodo-2'-deoxyuridine into the DNA of HeLa cells and the induction of alkaline phosphatase activity. (1/37241)

Inhibition of DNA synthesis during the period of exposure of HeLa cells to 5-iodo-2'-deoxyuridine (IUdR) inhibited the induction of alkaline phosphatase activity. This finding, taken together with previous findings that IUdR did not induce alkaline phosphatase activity in the presence of 2-fold molar excess thymidinemonstrated that IUdR incorporation into DNA is correlated with the increase in alkaline phosphatase activity. With the exception of an interim period described in the text, induction of alkaline phosphatase activity was linearly related to medium concentrations of IUdR of up to at least 3 muM. However, the extent of IUdR substitution in DNA did not appear to be related to the degree of enzyme induction. Alkaline phosphatase activity continued to increase at medium concentrations of IUdR from 1 to 3 muM, while little further substitution of DNA occurred.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (2/37241)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (3/37241)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite. (4/37241)

The reaction of HeLa-cell 5.8S rRNA with NaHSO3 under conditions in which exposed cytidine residues are deaminated to uridine was studied. It was possible to estimate the reactivities of most of the 46 cytidine residues in the nucleotide sequence by comparing 'fingerprints' of the bisulphite-treated RNA with those of untreated RNA. The findings were consistent with the main features of the secondary-structure model for mammalian 5.85S rRNA proposed by Nazar, Sitz, & Busch [J. Biol. Chem (1975) 250, 8591--8597]. Five out of six regions that are depicted in the model as single-stranded loops contain cytidine residues that are reactive towards bisulphite at 25 degrees C (the other loop contains no cytidine). The cytidine residue nearest to the 3'-terminus is also reactive. Several cytidines residues that are internally located within proposed double-helical regions show little or no reactivity towards bisulphite, but the cytidine residues of several C.G pairs at the ends of helical regions show some reactivity, and one of the proposed loops appears to contain six nucleotides, rather than the minimum of four suggested by the primary structure. Two cytidine residues that are thought to be 'looped out' by small helix imperfections also show some reactivity.  (+info)

Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. (5/37241)

Studies of the actin-based motility of pathogens have provided important insights into the events occurring at the leading edge of motile cells [1] [2] [3]. To date, several actin-cytoskeleton-associated proteins have been implicated in the motility of Listeria or Shigella: vasodilator-stimulated phosphoprotein (VASP), vinculin and the actin-related protein complex of Arp2 and Arp3 [4] [5] [6] [7]. To further investigate the underlying mechanism of actin-tail assembly, we examined the localization of components of the actin cytoskeleton including Arp3, VASP, vinculin and zyxin during vaccinia, Listeria and Shigella infections. The most striking difference between the systems was that a phosphotyrosine signal was observed only at the site of vaccinia actin-tail assembly. Micro-injection experiments demonstrated that a phosphotyrosine protein plays an important role in vaccinia actin-tail formation. In addition, we observed a phosphotyrosine signal on clathrin-coated vesicles that have associated actin-tail-like structures and on endogenous vesicles in Xenopus egg extracts which are able to nucleate actin tails [8] [9]. Our observations indicate that a host phosphotyrosine protein is required for the nucleation of actin filaments by vaccinia and suggest that this phosphoprotein might be associated with cellular membranes that can nucleate actin.  (+info)

Bcl-2 regulates amplification of caspase activation by cytochrome c. (6/37241)

Caspases, a family of specific proteases, have central roles in apoptosis [1]. Caspase activation in response to diverse apoptotic stimuli involves the relocalisation of cytochrome c from mitochondria to the cytoplasm where it stimulates the proteolytic processing of caspase precursors. Cytochrome c release is controlled by members of the Bcl-2 family of apoptosis regulators [2] [3]. The anti-apoptotic members Bcl-2 and Bcl-xL may also control caspase activation independently of cytochrome c relocalisation or may inhibit a positive feedback mechanism [4] [5] [6] [7]. Here, we investigate the role of Bcl-2 family proteins in the regulation of caspase activation using a model cell-free system. We found that Bcl-2 and Bcl-xL set a threshold in the amount of cytochrome c required to activate caspases, even in soluble extracts lacking mitochondria. Addition of dATP (which stimulates the procaspase-processing factor Apaf-1 [8] [9]) overcame inhibition of caspase activation by Bcl-2, but did not prevent the control of cytochrome c release from mitochondria by Bcl-2. Cytochrome c release was accelerated by active caspase-3 and this positive feedback was negatively regulated by Bcl-2. These results provide evidence for a mechanism to amplify caspase activation that is suppressed at several distinct steps by Bcl-2, even after cytochrome c is released from mitochondria.  (+info)

The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. (7/37241)

The cellular protein p32 was isolated originally as a protein tightly associated with the essential splicing factor ASF/SF2 during its purification from HeLa cells. ASF/SF2 is a member of the SR family of splicing factors, which stimulate constitutive splicing and regulate alternative RNA splicing in a positive or negative fashion, depending on where on the pre-mRNA they bind. Here we present evidence that p32 interacts with ASF/SF2 and SRp30c, another member of the SR protein family. We further show that p32 inhibits ASF/SF2 function as both a splicing enhancer and splicing repressor protein by preventing stable ASF/SF2 interaction with RNA, but p32 does not block SRp30c function. ASF/SF2 is highly phosphorylated in vivo, a modification required for stable RNA binding and protein-protein interaction during spliceosome formation, and this phosphorylation, either through HeLa nuclear extracts or through specific SR protein kinases, is inhibited by p32. Our results suggest that p32 functions as an ASF/SF2 inhibitory factor, regulating ASF/SF2 RNA binding and phosphorylation. These findings place p32 into a new group of proteins that control RNA splicing by sequestering an essential RNA splicing factor into an inhibitory complex.  (+info)

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (8/37241)

Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. A stable complex containing both Zta and CBP could be isolated from lytically stimulated, but not latently infected RAJI nuclear extracts. Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1ADelta2-36), which fails to bind CBP. Zta bound directly to two related cysteine- and histidine-rich domains of CBP, referred to as C/H1 and C/H3. These domains both interacted specifically with the transcriptional activation domain of Zta in an electrophoretic mobility shift assay. Interestingly, we found that the C/H3 domain was a potent dominant negative inhibitor of Zta transcriptional activation function. In contrast, an amino-terminal fragment containing the C/H1 domain was sufficient for coactivation of Zta transcription and viral reactivation function. Thus, CBP can stimulate the transcription of latent EBV in a histone acetyltransferase-independent manner mediated by the CBP amino-terminal C/H1-containing domain. We propose that CBP may regulate aspects of EBV latency and reactivation by integrating cellular signals mediated by competitive interactions between C/H1, C/H3, and the Zta activation domain.  (+info)

Hypoxia plays a key role in tumour cell survival, invasion, and metastasis. An increasing number of studies have attempted to characterize the tumour response to hypoxia and to identify predictive markers of disease. Here we show that hypoxia increases tumour cell invasion and migration by the modulation of Rab11, an important molecule for vesicular trafficking. In our study, we found that Rab11, together with the activation of Rac1, could stimulate invasion and migration of cervical cancer cell lines HeLa/SiHa in hypoxia. Activation of Rac1 activity by hypoxia seems to be central to carcinoma invasion. We also found that these effects could be related to the integrin αvβ3. In addition, we studied the molecular pathway for this process. Our results showed that in cervical cancer cell... lines HeLa/SiHa, Rac1 activation in hypoxia could stimulate invasion and migration, and this process was mediated by integrin αvβ3-mediated FAK and PI3K phosphorylation. Furthermore, hypoxia induced a ...
BioAssay record AID 87350 submitted by ChEMBL: Compound was tested for antiviral activity in HeLa Cell cultures against herpes simplex virus type 1 (HSV-1).
HeLa cells in culture. Light microscopy footage of the movement, division and death of HeLa cervical cancer cells. HeLa cells are a continuously cultured cell line of human cancer cells. They are immortal, in that they can divide an unlimited number of times. However, they are also poorly differentiated and lack some of the characteristics of normal cells. HeLa cells are widely used in biological and medical research. They all originate from a sample taken in 1951 from cervical cancer patient Henrietta Lacks, after whom they are named. - Stock Video Clip K005/6160
HeLa cell. Coloured scanning electron micrograph (SEM) of a cultured Hela cell (centre) on MDCK kidney cells (background). HeLa cells are a continuously cultured cell line of human cancer cells, which are immortal and so thrive in the laboratory. Numerous microvilli (string-like) can be seen, which cancer cells use to enable them to move around and spread (metastasise) from one area to another. MDCK cells are a strain of cultured, normal kidney epithelial cells. - Stock Image C011/1346
BioAssay record AID 506767 submitted by ChEMBL: Binding affinity to SAP145 in SAP145-targeting siRNA-treated human HeLa cells at 1 uM by fluorescent microscopy.
SHSY5Y and HeLa cells were transiently transfected with scRNA (SHSY5Y/scRNA and HeLa/scRNA) or -Syn-siRNA (SHSY5Y/-Syn-siRNA and HeLa/-Syn-siRNA) for 48 h. in malignancy cells. RESULTS TTP overexpression promotes an elongation of mitochondria Previously, we reported that overexpression of TTP suppresses cellular proliferation [46, 47, 50] and induces a change in cell morphology from a mesenchymal shape to an epithelial shape [51]. Here we assessed whether TTP overexpression modifies mitochondrial morphology. To test this, SHSY5Y neuroblastoma cells and HeLa cervical carcinoma cells were transiently transfected with pcDNA6/V5-TTP (SHSY5Y/TTP and HeLa/TTP) or a control pcDNA6/V5 (SHSY5Y/pcDNA and HeLa/pcDNA) vector. After confirming the overexpression of TTP by RT-PCR and western blot analysis (Number ?(Figure1A),1A), mitochondria in the cells were stained with Mitotracker. Confocal microscopic imaging of mitochondria showed that TTP overexpression advertised the elongation of the mitochondrial ...
Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for tumor initiation and maintenance. They are also considered as an attractive target for advanced cancer therapy. Using a sphere culture method that favors the growth of self-renewal cells, we have isolated sphere-forming cells (SFCs) from cervical cancer cell lines HeLa and SiHa. HeLa-SFCs were resistant to multiple chemotherapeutic drugs and were more tumorigenic, as evidenced by the growth of tumors following injection of immunodeficient mice with 1 × 104 cells, compared with 1 × 106 parental HeLa cells required to grow tumors of similar size in the same time frame. These cells showed an expression pattern of CD44high/CD24low that resembles the CSC surface biomarker of breast cancer. We further demonstrated that HeLa-SFCs expressed a higher level (6.9-fold) of the human papillomavirus oncogene E6, compared with that of parental HeLa cells. Gene silencing of E6 with a lentiviral-short-hairpin RNA (shRNA)
Fig. 6. Mitotic events of cell division after disruption of mitochondria in the histone EGFP-H1 expressed cell. The disruption of a single mitochondrion by femtosecond laser irradiation had no influence on cell division or cell activity. The cell nuclei and mitotic chromosomes in HeLa cells were visualized using histone EGFP-H1. Mitochondria were stained with MitoTracker Red. Confocal fluorescence image and transmission image (a) before and (b) after femtosecond laser irradiation with 0.39 nJ/pulse (exposure time: 32 ms). The yellow arrow indicates the irradiation point. (c)-(f) Time-lapse confocal images and transmission images. The mitotic events of cell division in the irradiated cells proceeded normally. Scale bar: 20 μm ...
Im pretty sure the fact that HeLa cells are abnormal has been known more or less since they were first isolated. That said, how they are abnormal has not. And this is very relevant - if researchers are using HeLa cells to study something, and HeLa cells are abnormal along that axis, then their data are suspect. So knowing how they are normal, and abnormal, is important information. This is why the news that the transformation of healthy non-cancerous cervical cells into HeLa cells happened due to human papilloma virus infection - that is, Henrietta Lacks (the source of the cells) had HPV, which gave her the cancer that ultimately killed her. This is noteworthy because it establishes the mutation(s) and phenotype(s) that result when cervical cells are infected with HPV (with ramifications both for work with HeLa and other cervical cancer lines AND for care for patients).. Remember also that HeLa was the very first immortalized cell line. Studies on HeLa led to the immortalization of other cells, ...
Hypoxia plays a key role in tumour cell survival, invasion, and metastasis. An increasing number of studies have attemptedto characterize the tumour response to hypoxia and to identify predictive markers of disease. Here we show that hypoxiaincreases tumour cell invasion and migration by the modulation of Rab11, an important molecule for vesicular trafficking.In our study, we found that Rab11, together with the activation of Rac1, could stimulate invasion and migration of cervicalcancer cell lines HeLa/SiHa in hypoxia. Activation of Rac1 activity by hypoxia seems to be central to carcinoma invasion.We also found that these effects could be related to the integrin αvβ3. In addition, we studied the molecular pathway for thisprocess. Our results showed that in cervical cancer cell lines HeLa/SiHa, Rac1 activation in hypoxia could stimulateinvasion and migration, and this process was mediated by integrin αvβ3-mediated FAK and PI3K phosphorylation.Furthermore, hypoxia induced a dramatic increase ...
Light micrograph of dividing HeLa cells. Chromosomes in the cell nucleus (purple), microtubules in the cell cytoskeleton (tubulin; green), and actin (red) are visible here. In the cell in the centre of the image, condensed chromosomes (purple) have aligned and attached to the spindle (green). During anaphase (one of the stages of nuclear division in mitosis), the spindle pulls the condensed chromosomes to opposite poles of the cell. The cell will finish dividing its nuclear material and then cytoplasm in order to physically separate into two daughter cells. HeLa cells are an immortal human epithelial cell line derived from a cancerous tumour of the cervix (adenocarcinoma). It was established in 1951 from a biopsy taken from Henrietta Lacks and was the first human cell line to survive and grow in the laboratory. Henriettas cells were originally used in this way without permission from her or her family which raises issues about ethics and privacy. HeLa cells have been used extensively around the world
Definition of HeLa cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is HeLa cell? Meaning of HeLa cell as a legal term. What does HeLa cell mean in law?
Background Cervical cancer is one of the most common cancers in women worldwide. Malignant tumors develop resistance mechanisms and are less sensitive to or do not respond to irradiation. With the development of high-throughput sequencing technologies, circular RNA (circRNA) has been identified in an increasing number of diseases, especially cancers. It has been reported that circRNA can compete with microRNAs (miRNAs) to change the stability or translation of target RNAs, thus regulating gene expression at the transcriptional level. However, the role of circRNAs in cervical cancer and the radioresistance mechanisms of HeLa cells are unknown. The objective of this study is to investigate the role of circRNAs in radioresistance in HeLa cells. Methods High-throughput sequencing and bioinformatics analysis of irradiated and sham-irradiated HeLa cells. The reliability of high-throughput RNA sequencing was validated using quantitative real-time polymerase chain reaction. The most significant circRNA
This study demonstrated the cytotoxic and cell growth inhibitory effect of a bioactive extract of |i|Funalia trogii|/i| (Berk.) Bond. et Singer, which was grown...
The quantitative study of cell morphology is of great importance as the structure and condition of cells and their structures can be related to conditions of health or disease. The first step towards that, is the accurate segmentation of cell structures. In this work, we compare five approaches, one traditional and four deep-learning, for the semantic segmentation of the nuclear envelope of cervical cancer cells commonly known as HeLa cells. Images of a HeLa cancer cell were semantically segmented with one traditional image-processing algorithm and four three deep learning architectures: VGG16, ResNet18, Inception-ResNet-v2, and U-Net. Three hundred slices, each 2000 × 2000 pixels, of a HeLa Cell were acquired with Serial Block Face Scanning Electron Microscopy. The first three deep learning architectures were pre-trained with ImageNet and then fine-tuned with transfer learning. The U-Net architecture was trained from scratch with 36, 000 training images and labels of size 128 × 128. The ...
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
Cell and Culture Conditions. The PKR wild-type MEFs [MEF/PKR(+/+)] and PKR-knockout MEFs [MEF/PKR(-/-)], provided by Dr. Glen Barber (University of Miami, Miami, FL), have been described previously (Yang et al., 1995). The human cervical cancer cell line HeLa and the human lung cancer cell lines H1299, H460, H226B, and A549 were maintained in our laboratory. The human bronchial epithelial (HBE) cell line was purchased from Clonetics (Walkersville, MD). All cells except for HBE cells were routinely cultured in Dulbeccos modified Eagles medium supplemented with 10% heat-inactivated fetal calf serum, 100 units/ml penicillin, and 100 mg/ml streptomycin and maintained in the presence of 5% CO2 at 37°C. HBE cells were cultured in serum-free keratinocyte medium (Invitrogen, Carlsbad, CA).. Chemicals and Antibodies. A chemical library with 10,000 compounds, including BEPP and its analog BECC, was obtained from ChemBridge Research Laboratories, Inc.. The chemicals in the library were provided at a ...
One cryopreserved vial of HeLa NucLight Red (nuclear-labelled RFP) expressing cells at a density of 1x106 cells/vial. Each vial contains a stable population of 1 million HeLa cells expressing the NucLight Red fluorescent protein restricted to the nucleus. Parental HeLa cells were purchased from ATCC (Cat# CCL-2). HeLa
Although it is widely believed that eukaryotic DNA is looped by attachment to a nucleoskeleton, there is controversy about its composition and which sequences are attached to it. As most nuclear derivatives are isolated using unphysiological conditions, the criticism that attachments seen in vitro are generated artifactually has been difficult to rebut. Therefore we have re-investigated attachments of chromatin to the skeleton using physiological conditions. HeLa cells are encapsulated in agarose microbeads and lysed using Triton in a physiological buffer. Then, most chromatin can be electroeluted after treatment with a restriction enzyme to leave some at the base of the loops still attached. Analysis of the size and amounts of these residual fragments indicates that the loops are 80-90kbp long. The residual fragments are stably attached, with about 1kbp of each fragment protected from nuclease attack. This is very much longer than a typical protein-binding site of 10-20bp.
Cervical cancer is a common female malignancy of global dimensions. MicroRNAs (miRNAs) play crucial roles in the development, differentiation, proliferation, and apoptosis of tumors. The non-coding RNA MALAT1 participates in various physiological processes that are important for proper functioning of the body. Here, we analyzed the expression of miRNA-143 and MALAT1 in HeLa cells to evaluate their roles in the occurrence and metastasis of cervical cancer. HeLa cells were divided into five groups depending on the treatment conditions, namely, transfected with miRNA-143, MALAT1, miRNA-143 inhibitor and the MALAT1 inhibitor, and the untreated control. Reverse transcription-polymerase chain reaction was used to analyze the expression of miRNA-143 and MALAT1, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess proliferation, the trans-well assay to study cell invasion and migration, and western blot to analyze the levels of E-cadherin and vimentin. The proliferation of HeLa
While both the Rabenosyn-5 and Vps45 orthologs appear to coordinate endocytic regulatory functions in yeast and invertebrates, their relationship in mammalian cells is not well understood. Leaving out -L would be equivalent to L0, or specifying that the pool level was backup01. To assess the impact of TBC1d5 loss on VPS35 recruitment to endosomes, TBC1d5 KO cells were mixed with control HeLa cells at a ratio of 1:1 and the cells were simultaneously stained with TBC1d5, VPS35 and EEA1. 1 ). This approach, used by most system emulators, allows the emulator to run an arbitrary guest operating system without modifications because guest OS is not aware that it is not running on real hardware. This is consistent with a proposed role for the vps pathway in VP40 vesicular release. Untuk konsultasi konfigurasi server custom isp for a dns server mendapatkan diskon terbaik (untuk pembelian cloud resources dalam jumlah banyak), silahkan hubungi free terraria server download. BlueHost grew enormously in the ...
TY - JOUR. T1 - On the spatial organization of ubiquitin-dependent proteolysis in HeLa cells. AU - Wójcik, Cezary. PY - 1997/1/1. Y1 - 1997/1/1. KW - HeLa cells. KW - proteasome. KW - proteolysis. KW - ubiquitin. UR - http://www.scopus.com/inward/record.url?scp=0342941148&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0342941148&partnerID=8YFLogxK. M3 - Article. C2 - 9151102. AN - SCOPUS:0342941148. VL - 35. SP - 117. EP - 118. JO - Folia Histochemica et Cytochemica. JF - Folia Histochemica et Cytochemica. SN - 0015-5586. IS - 2. ER - ...
INA-UV induced apoptosis in Hela cells is also caspase dependent. A. Hela cells, pre-incubated or not with 40 μM ZVADfmk were treated with indicated amounts of
HeLa cells were washed with cold PBS and lysed in cell lysis buffer (20-mM Tris-HCl, pH 8.0, 150-mM NaCl, 2-mM EGTA, 0.5-mM EDTA, 0.5% NP-40, 5-mM NaF, 1-mM Na3VO4, 1-mM PMSF, and 500× protease inhibitor cocktail; Calbiochem) for 20 min on ice. For Usp16/Plk1 coIP assays, cell lysates were mixed with anti-Usp16 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Usp16 1-257 aa) or mouse anti-Plk1 antibodies (06-813; EMD Millipore) and 15 µl protein A-Sepharose beads (75% slurry) and incubated at 4°C for 1 h. After washing with lysis buffer, the beads were harvested and suspended in Laemmli sample buffer. For Plk1/BubR1 coIP assays, cell lysates were mixed with anti-Plk1 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Plk1 326-end). For GST pull-down assays, HeLa cell lysates were incubated with 5 µg of soluble GST or GST-fused proteins bound to 15 µl glutathione-Sepharose beads (75% slurry) at 4°C for 1 h. After washing with lysis buffer, ...
Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options. Just like model […]. The post Top 5 of the most commonly used cell lines! appeared first on Bitesize Bio.. ...
AC0206: Western Blot (1:1000) of over-expressed TMX with positive and negative controls on HeLa Cells. TMX is the 34kDa band. The upper band (~38 kDa) is myc-tagged TMX. The lower band at~28 kDa (*) is a non-specific band.. ...
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Kinetics of B-fragment transport from EE/RE to the Golgi apparatus. (A) Confocal microscopy on living HeLa cells. Fluorophore-labeled B-fragment was internalize
Here we present a new approach to aqueous hydrazoic acid detection through the synthesis and evaluation of an alkyne-based fluorescent probe, which could be applied in the monitoring of hydrazoic acid in both living Hela cells and larval zebrafish. This probe can also serve as an early warning automaton whic
目的:探讨反义脱氧寡核苷酸封闭HSP70基因对体外宫颈癌 HeLa 细胞增殖、凋亡及化疗敏感性的影响。方法:1)将体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、反义寡核苷酸处理组(AS组)、正义寡核苷酸处理组(S组)、随机寡核苷酸处理组(R组),每组各5例,分别转染体外培养的宫颈癌 HeLa 细胞,采用Western免疫印迹检测各组细胞HSP70蛋白表达。2)将顺铂处理体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、单纯顺铂处理组(Cis组)、反义寡核苷酸+顺铂处理组(AS +Cis组)、正义寡核苷酸+顺铂处理组(S +Cis组)、随机寡核苷酸+顺铂处理组 (R +Cis组),每组各8例。采用四甲基偶氮唑蓝光吸收法(methyl thiazolyl tetrazolium,MTT) 法检测 HeLa 细胞的生长抑制率; 流式细胞术检测 HeLa 细胞的凋亡率。结果:1)Ctrl组、AS组、S组、R组HSP70灰度比值分别为1.365±0.187,0.379±0.134,1.403±0.163和1.410±0
When Professor Gertrude Buehring steps into her lab in Koshland Hall, she is never met by HeLa cells. In fact, she makes a point of it. We never grow them, she says. I wouldnt want to take that risk, actually.. Buehring, a professor of virology in the School of Public Health, has a reason to be wary of HeLa. Both her PhD and postdoc careers were spent working at UC Berkeleys Cell Culture Laboratory housed in the Naval Biosciences Laboratory in Oakland, a cell repository funded by the federal government that characterized and maintained cell lines for research scientists. She happened to be working there at a time when Dr. Walter Nelson-Rees, the co-director, was working hard to expose HeLas misdeeds. The vigorous cells crime? The contamination of other, less hardy cell lines.. Nelson-Rees was not the first to suspect contamination by HeLa cells. In the 1960s, Dr. Stanley Gartler, a research geneticist, released the initial HeLa bomb. Gartler had discovered that the 18 different cell ...
This paper reviews several different cell types and cell preparation approaches using standard cells, such as, CHO cells, EL4 cells, SF9 cells, and HELA cell cultures.
Ran-Binding Protein M (RanBPM) has been previously shown to inhibit c-Raf expression, however how this was achieved remains unclear. c-Raf is the central component of the extracellular signal-regulated kinase (ERK) pathway which has been linked to many cancer types. Furthermore, RanBPM was recently identified as part of the E3 ubiquitin ligase complex and the CTLH (C-terminal to LisH) complex (McTavish et al., 2019). Lastly, RanBPM has been linked to various signaling pathways related to numerous cellular processes which include - apoptosis, cell adhesion, migration, transcription, nuclear-cytoplasmic transport and also plays a significant role during development (Salemi et al., 2017). ...
Human IFITM1 Knockout HeLa Cell Lysate parental control lysate. CRISPR/Cas9 generated negative control for antibody validation in WB.
The assembly of a rotating HeLa cell/gold rod aggregate at an acoustic nodal line in the xy plane. The video was taken under 500X overall magnification except for 00:23 - 00:32 and 01:16 - 01:42, where a 200X overall magnification was used.
TY - JOUR. T1 - Anomalous structures in the cytoplasm of HeLa cells cultured in the presence of 5-bromodeoxvuridine.. AU - HENDEE, W. R.. AU - ZEBRUN, W.. AU - BONTE, F. J.. PY - 1963/6. Y1 - 1963/6. UR - http://www.scopus.com/inward/record.url?scp=84883840494&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84883840494&partnerID=8YFLogxK. M3 - Article. C2 - 13953692. AN - SCOPUS:84883840494. VL - 17. SP - 675. EP - 680. JO - Journal of Cell Biology. JF - Journal of Cell Biology. SN - 0021-9525. ER - ...
Could anyone tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment.Thanks a lot!. ...
The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cytotoxicity against HeLa cellscompared to the free ICD-85. The IC50 value at 72 h was reduced from 25 ± 2.9 μg/mL for free ICD-85 to 15.5 ± 2.4 μg/mL for ICD-85 NPs. However, LDH assay demonstrated that ICD-85 has dose-dependent cytotoxicity on HeLa cells while ICD-85 NPs exhibited weaker cytotoxicity on same cells. The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. Moreover, caspase-8 assay analysis demonstrated that the ICD-85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. Our results demonstrated that the
Background and AimCervical and breast cancer are considered to be the most common cause of death among Iranian women. Various studies have shown that probiotics are effective fighting cancer. The aim of the present study was to investigate the effect of Lactobacillus brevis on apoptosis and casp (casp3, casp8) gene expression in HeLa cancer cells. Materials and MethodsIn this study, L. brevis bacteria were collected from the vaginal fluid of females referred to Alzahra Hospital and isolated and cultured in MRS agar medium. Inhibition of HeLa cancer cell proliferation by bacteria was evaluated by MTT assay. Apoptosis of cancer cells was measured by fluorescent microscopy using DAPI method. Finally, expression of Caspase and akt genes was measured by Real time PCR.ResultsThe results indicated that L. brevis had the same effect on HeLa cancer cells (P=0.42, P=0.26). On the other hand, there was no significant increase in akt gene expression (p> 0.05).ConclusionLactobacillus brevis bacteria can be
NDRG2, a member of N-Myc downstream regulated gene family, plays some roles in cellular stress, cell differentiation and tumor suppression. We have found that NDRG2 expression in cervical cancer Hela cells increases significantly upon stimulation with cisplatin, the most popular chemotherapeutic agent currently used for the treatment of advanced cervical cancer. This interesting phenomenon drove us to evaluate the role of NDRG2 in chemosensitivity of Hela cells. In the present study, RNA interference was employed to down-regulate NDRG2 expression in Hela cells. RT-PCR and Western blot were used to detect expression of NDRG2, Bcl-2 and Bax in cancer cells. Real-time PCR was applied to detect miR-15b and miR-16 expression levels. Drug sensitivity was determined with MTT assay. Cell cloning efficiency was evaluated by Colony-forming assay. Apoptotic cells were detected with annexin V staining and flow cytometry. In vitro drug sensitivity assay revealed that suppression of NDRG2 could sensitize Hela cells
Epithelial-to-mesenchymal transition (EMT) renders cervical cancer cells more susceptible to metastasis, and reduced expression levels of E-cadherin have been shown in this malignancy (9). E-cadherin is a pivotal molecule involved in epithelial cell adhesion, aberrant expression of which has been demonstrated in various human malignancies as a result of irreversible (genetic) and reversible (epigenetic) modifications of the E-cadherin gene (CDH1), such that the epigenetic ones have been more implicated in E-cadherin downregulation (10).. In this study, we evaluated the impact of oxamflatin on HeLa cell line by determining the expression level of E-cadherin. Our results demonstrated that oxamflatin treatment leads to a time- and concentration-dependent increase in E-cadherin expression in cervical cancer cell lines.. There is ample evidence reporting that HDACIs could be applied to re-express silenced genes (9, 11). For example, valproic acid (VPA), as a HDAC inhibitor, led to the re-expression ...
TY - JOUR. T1 - Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting. AU - Yamahara, Yumi. AU - Nagasawa, Naotsugu. AU - Taguchi, Mitsumasa. AU - Oshima, Akihiro. AU - Washio, Masakazu. PY - 2018/1. Y1 - 2018/1. N2 - Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is ...
The antineoplastic activity of chloroform stem bark extract of rohituka, Aphanamixis polystachya (APE) used traditionally to treat spleen and liver tumors was evaluated in cultured HeLa cells by clonogenic and micronucleus assays. Treatment of HeLa cells with 0, 5, 10, 25, 50, 75 or 100µg/ml APE for different times caused a concentration-dependent reduction in the cell survival up to 6 h post-treatment (p|0.005) followed by a non-significant decline in the cells treated with APE for 24 h. Therefore, 6h treatment time was considered as an optimum time for APE exposure and further studies were carried out using this treatment time. Exposure of HeLa cells to different concentrations of APE resulted in a concentration-dependent decline in the cell viability and 100µg/ ml APE resulted in 72% cell death when compared with the non-drug treated control group. These results were further confirmed by clonogenic assay where, APE treatment caused a concentration-dependent decline in the clonogenicity of HeLa
Carcinoma of the cervix is the second most common cancer among women worldwide especially in the developing world. Due to severe reported cytotoxic tendenciesby anticancer drugs, newer therapies from natural products are desirable. This work therefore aims to partially purify pro-apoptotic agents from indigenous natural products and to investigate the mechanism of action of the selected plant fractions.Sixteen indigenous medicinal plants were collected from South West Nigeria. Plant extracts were fractionated into hexane (HF), chloroform (CF), ethylacetate (EAF), methanol (MF) and water (WF) fractions using solvent-solvent partitioning and polyamide adsorption chromatography. The resultant ninety-six test fractions were screened for lethality potential using brine shrimp lethality (BSL) assay. Fractions with LC50 less than 10 ug/ml were subjected to Water Soluble Tetrazolium (WST-1) cytotoxicity assay and apoptosis evaluations using: morphological assessment, flow cytometric analysis of ...
Since FTO was recognized as the first m6A demethylase, the understanding of its biological function has been widely expanded. However, the role of FTO in cervical cancer tumorigenesis remains unclear. In this study, we first analyzed the expression of FTO in two independent human cancer datasets and evaluated the correlation between FTO level and cervical cancer progression. Using small hairpin RNA technology, we explored the function of FTO in cervical cancer cell line Hela and SiHa cells, respectively. We then determined the FTO targets by performing transcriptional profile with FTO deficient and competent Hela cells, and finally validated these targets with ribosome profiling and functional rescue experiments. Our data suggested that FTO was frequently overexpressed in human cervical cancer tissues and highly correlated with cervical cancer progression. FTO serves as an oncogenic regulator for cervical cancer cells proliferation and migration which is vastly depended on its demethylase activity.
TY - GEN. T1 - Electrical characterization of single HeLa cell using 2D simulation and spectroscopy measurement. AU - Wang, M. H.. AU - Chen, M. K.. AU - Jang, L. S.. PY - 2008/12/1. Y1 - 2008/12/1. N2 - The electrical properties of single cells provide fundamental insights into their pathological condition and are therefore of immense interest to medical practitioners. Accordingly, this study captures single HeLa cells using a microfluidic device and then measures their impedance properties using a commercial impedance spectroscopy system. The experimental system is modeled by an equivalent electrical circuit and COMSOL simulations are then performed to establish the conductivity, permittivity and impedance of single HeLa cells under various operational frequencies and voltages. At an operational voltage of 0.2 V, the maximum deviation between the experimental and simulation results for the magnitude and phase of the HeLa cell impedance is found to be 9.5% and 4.2%, respectively. In general, ...
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a marked stimulation of inorganic [32P]orthophosphate incorporation into HeLa-cell phosphatidylcholine (PC), phosphatidylethanolamine (PE), and lysophosphatidylethanolamine. The increased incorporation of inorganic [32P]orthophosphate into PE and lysophosphatidylethanolamine in the presence of TPA was not associated with an increase in PE synthesis as detected by the incorporation of [3H]serine or [3H]ethanolamine. The PC-specific exchange protein from beef liver was used to insert PC labeled with [3H]choline, inorganic [32P]orthophosphate, or [14C]arachidonic acid plus [3H]palmitic acid into the outer monolayer of intact HeLa cell membranes. Radioactivity from the latter two compounds was rapidly incorporated into PE and lysophosphatidylethanolamine; the incorporation was stimulated by TPA. It was concluded that TPA stimulated the formation of PE by base exchange between ethanolamine and PC.. ...
This is too long for a comment, so I have to write it in here:. Mostly this is because other cell lines are more practical in the lab.. Stem cells are much more tricky to maintain - especially if you want to keep their stem cell properties. They only grow very slow, tend to differentiate when they get too much stress, cellular signals and so on and need special media (these need to be defined, so that they are not containing factors which promote differentiation for example). For everyday experiments (like expression profiles, experiments on the effects of certain substances on the growth, gene expression or whatever of cells) normal cell lines are much more suited as these are much easier to keep in the lab using standard media. Additionally,experiments can be more easily repeated and reproduced, since these cells should behave always the same.. ...
Twenty years ago this morning, I had to defend a body of work that contained this paragraph on page 24: HeLa cells are a human cervical carcinoma cell line having a doubling time of 24 hr and were obtained from Dr. Bert Flanegan, Dept. of Immunology and Microbiology, University of Florida. HeLa cells were maintained…. ...
The cationic complexes (1,2-diaminoethane)(maltolato)platinum(II) ([Pt(en)(ma)]+) and (1R,2R-1,2-diaminocyclohexane)(maltolato)platinum(II) ([Pt(R,R-DACH)(ma)]+) have been prepared and the structure of [Pt(R,R-DACH)(ma)]NO3 has been determined by single crystal X-ray diffraction. The geometry of the metal in [Pt(R,R-DACH)(ma)]NO3 is essentially square planar and the maltolate ligand has a geometry similar to other chelate complexes involving this ligand. The cytotoxicities of the compounds have been assessed in the human cell lines HeLa and K562 and the IC50 values are approximately 32 microM in HeLa cells and 26 microM in K562 cells. In these cell lines the cytotoxicity of cisplatin is higher than the maltolate complexes by a factor of 2 to 3 whereas the cytotoxicity of carboplatin is lower than the maltolate complexes ...
TY - JOUR. T1 - Fractions of HeLa DNA differing in their content of guanine + cytosine. AU - Schildkraut, Carl L.. AU - Maio, Joseph J.. PY - 1969/12/14. Y1 - 1969/12/14. N2 - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.. AB - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C ...
HeLa whole cell lysate (Positive control), 0.1 mg. This whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.
HeLa whole cell lysate Lysates datasheet (ab29545). Abcam offers quality products including antibodies, assays and other reagents.
The Mab K1 was generated by immunization of mice with the human ovarian carcinoma cell line OVCAR-3 (3) . The reactivity of Mab K1 against a variety of different human tumor cell lines was tested using immunofluorescence (3) . It showed reactivity with several ovarian cancer cell lines, including OVCAR-2, OVCAR-3, OVCAR-5, A-1847, and SKOV3; cervical cancer cell lines HeLa and KB; and gastric cell lines AGS and HTB103. No reactivity was detected with breast, colon, or prostate cancer cell lines. Care must be taken with the use of Mab K1 because its reactivity is acid labile (5) .. The reactivity of Mab K1 against normal human tissues was tested by immunohistochemistry using cryostat tissue sections (3) . Most normal tissues showed no reactivity with Mab K1, with the exception of mesothelial cells that line the peritoneal, pleural, and pericardial cavities. There was also weak reactivity with the basal cells of the trachea and cells in the Fallopian tubes. A similar immunoreactivity was seen in ...
The relationships between DNA damage from UV radiation, alkylating drugs and the methylated xanthines (MX) have been studied in normal and malignant rodent and human cells. A comparison of the level of DNA excision repair (repair replication and unscheduled DNA synthesis) confirms that some forms of alkylating-agent damage (probably mono-filar DNA adducts) are less completely removed by both normal and malignant rodent cells than by their human counterparts, rendering rodent cells more susceptible to the toxic potential of unexcised lesions. The toxicity of alkylating agents can be increased by the presence of several MXs during the period of DNA replication which follows infliction of the damage. Human cells appear capable of excising more DNA damage, rendering them somewhat less susceptible to enhancement of cytotoxicity by MX. This resistance of human cells is only quantitative, however, since 2 human cancer cell lines (HeLa and HT-29) could be sensitized to a variety of alkylating agents by ...
a,b, Western blotting analysis of miR-20a target genes in response to increasing doses of miR-20a mimic (a) or antagomir (b). c,d, RT-qPCR analysis of miR-20a levels in HeLa cells transfected with miR-20a mimic (c) or antagomir (d). e,f, RT-qPCR analysis of DAPK3 mRNA levels in HeLa cells transfected with miR-20a mimic (e) or antagomir (f). g, Luciferase reporter constructs containing the miR-20a MRE from DAPK3 (native) in Renilla 5 UTR or mutated to restore base-pairing in the 5 end (seed) coupled with progressive mismatches (3MM+seed) in the 3 end. h,i, Results of the luciferase reporter assays (h) and quantification of luciferase mRNA (i). j,k, mRNA levels of luciferase reporters containing individual MREs from 4 indicated genes that function in both CDS and 3UTR (j) or those that function only in CDS (k), as shown in main Fig. 2e,f. l,m, Luciferase activities from the reporters containing individual MREs from 4 indicated genes that function only in CDS in response to co-transfected ...
We have been using HeLa cells in my laboratory since 1982, when I arrived at Columbia University Medical Center fresh from postdoctoral work with David Baltimore at MIT. I brought with me a line of HeLa cells and used them for 30 years for our research on viruses. Here is a story of how we lost the cells and then found them ten months later.. As everyone knows, the continuous HeLa cell line was derived from a cervical tumor taken from Henrietta Lacks in 1951 (if you dont know the story, you should read Rebecca Skloots The Immortal Life of Henrietta Lacks, or my shorter summary). When I arrived at the Baltimore lab in 1979, they were using cells derived from the S3 clone of HeLa cells that had been produced by Philip Marcus in the 1950s. I write derived from because someone at MIT had further cloned the S3 line and selected one that was particularly susceptible and permissive to poliovirus infection. This was the cell line that I took with me to Columbia in 1982.. Because we use so many HeLa ...
Dr. Karen Resendes, Westminster College assistant professor of biology, recently published two articles on HeLa cell research, one of which was in collaboration with Gretchen Diffendall 14.
In this study, the cell lines HeLa, 3T3-Swiss albino, CHO-Kl, and ARPE-19 were cultured and co-incubated with variable concentrations of S-phosphorylated thiols and Nacetylcysteine. Using MTT assays, the cultures were ...
An attempt was made to induce calciphylaxis and direct calcifying reaction according to Selye2in HeLa cell cultures. With our experimental conditions, we obtained neither a calciphylaxis nor a...
Derived from Henrietta Lacks in 1951, HeLa is an immortal cell line that is useful for its remarkable prolificity and durability. It was the inspiration for the national bestseller, The Immortal Life of Henrietta Lacks, written by the incredibly attractive Rebecca Skloots. Because of their high reproductive rate, HeLa cells are a good beginner transfection cell line to test the efficacy of the CPNP delivery of the origami. ...
In the second of three interviews with Bill Andrews at the 2016 AMMG, we discuss the Henrietta Lacks (or HeLa) cervical cancer cells commonly used in cell research. Dr. Andrews also discusses why telomere erosion is less a factor in mice aging.. ...
The final argument Ill advance against HeLa in cancer research is the fact that it has been in culture so long. HeLa should be seen as something like a bizarre new species found only in laboratories, not a representative of human cancer. All that time in culture has certainly enabled HeLa (or all the different HeLa) to evolve far away from the tumor which killed Henrietta Lacks. Again, this is a flaw in all in vitro propagated cell line studies, but given that HeLa is the oldest it is likely the most affected (granted, what wed really like to know is actual time in culture). Patient-derived xenografts are far more likely to be informative of tumors, but their expense and newness has meant they havent yet fully displaced in vitro lines from research programs ...
The final argument Ill advance against HeLa in cancer research is the fact that it has been in culture so long. HeLa should be seen as something like a bizarre new species found only in laboratories, not a representative of human cancer. All that time in culture has certainly enabled HeLa (or all the different HeLa) to evolve far away from the tumor which killed Henrietta Lacks. Again, this is a flaw in all in vitro propagated cell line studies, but given that HeLa is the oldest it is likely the most affected (granted, what wed really like to know is actual time in culture). Patient-derived xenografts are far more likely to be informative of tumors, but their expense and newness has meant they havent yet fully displaced in vitro lines from research programs ...
FIG. 2. PP2A mediates RPA32 dephosphorylation at T21 and S33. (A) HeLa cells were transfected with a mock siRNA oligonucleotide or with oligonucleotides against the catalytic subunits of PP1, PP2A, PP4, PP5, and PP6. Twenty-four hours posttransfection, cells were pulse treated with HU (0.2 mM, 24 h), and RPA32 dephosphorylation at T21/S33 was compared at the indicated time points. (B) HeLa cells transfected with control siRNA or siRNA against PP2A/C were pulse treated with HU (0.2 mM, 24 h). At 0 to 12 h postrelease, the cells were fixed and costained with DAPI and anti-RPA32pS33 and anti-γ-H2AX antibodies. The DNA damage-inducible foci were visualized by immunofluorescence microscopy, and the percentage of phospho-RPA32 and γ-H2AX focus-positive cells was determined, normalized, and plotted. The error bars represent the standard deviations from three independent experiments. (C) Immunoprecipitation (IP) assays were performed in unperturbed cells or cells recovering from exposure to pulses of ...
Mitochondria in HeLa cells are identified by mito-YFP (green) following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-c...
This section illustrates a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye, Dil, which targets membranes. The absorption maximum of Dil is 549 nanometers and the emission maximum occurs at 565 nanometers (in the yellow region of the visible light spectrum). Fluorescence intensity arising from labeled cytoskeletal membrane components is quite evident in the image even through the probe emission peak is 30 nanometers below the Y-2E/C filter set dichromatic mirror cut-on wavelength.
hi i,m rodrigo from chile and i have the same question of which proteins can be secreted by HeLa cells into the growth medium, if you know the answer please send to me a this mail rodrigosakuragi from gmail.com ...
However, the HeLa cells have also caused much pain, suffering, and confusion for the Lacks family. The Lackss family, for example, saw no profits despite being asked to contribute blood for subsequent research about which they were not adequately informed. Henrietta Lacks was treated during a time when medical ethics were very different.
Lab Reagents Human IgG antibody Laboratories manufactures the hela difluo invivogen reagents distributed by Genprice. The Hela Difluo Invivogen reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact InVivoGen. Other Hela products are available in stock. Specificity: Hela Category: Difluo Group: Invivogen Invivogen information ...
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
DataMed is a prototype biomedical data search engine. Its goal is to discover data sets across data repositories or data aggregators. In the future it will allow searching outside these boundaries. DataMed supports the NIH-endorsed FAIR principles of Findability, Accessibility, Interoperability and Reusability of datasets with current functionality assisting in finding datasets and providing access information about them.
Human ACE2 gene was integrated into the chromosomes of HeLa cells to form an engineered cell line, hACE2-HeLa, that stably expresses ACE2 on the cell surface.
Fluorescence images of control and Si-RNA transfected HeLa cells treated with nocodazole and allowed to recover. Mitotic cells were stained for alpha-...
Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells--taken without her knowledge--became one of the most important tools in medicine. The first immortal human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. If you could pile all HeLa cells ever grown onto a scale, theyd weigh more than 50 million metric tons--as much as a hundred Empire State Buildings. HeLa cells were vital for developing the polio vaccine; uncovered secrets of cancer, viruses, and the atom bombs effects; helped lead to important advances like in vitro fertilization, cloning, and gene mapping; and have been bought and sold by the billions ...
comes out next month, written about the woman and the cells which should have made her famous. Rebecca Skloot been researching it for something like 10 years and its got some great reviews. Im going to pre-order a copy, and if youd like to know more about HeLa cells and Henrietta Lacks, do the same! Its a story that deserves to be heard, and if there are enough pre-orders, Amazon will help promote the book. Plus its 30% off at the moment. What more could you want? Heres the blurb.. Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells-taken without her knowledge-became one of the most important tools in medicine. The first immortal human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. If you could pile all HeLa cells ever grown onto a scale, theyd weigh more than 50 million metric tons-as much as a hundred Empire State ...
Figure 1: Effects of PKR on the proliferation and translation. (a) Effects of PKR on the proliferation of HeLa cells. After being transfected with plasmids PKR, PKR siRNA, or GFP, HeLa cells were plated in multiple wells of a 96-well plate and grown for 24 hr for cell proliferation assays. Cells from the sample preparations were collected for immunoblotting. Proliferation rate of the control sample was normalized to 100%. PKR, WT PKR; si-PKR, PKR siRNA; Ctrl, GFP. Upper panel, averaged data (N=4 ...
Lysates of HeLa cells treated with UV or untreated cells for use as controls in western blotting to analyze UV-induced DNA damage and/or apoptosis.
When we work with cell lines in the lab, we often work with HeLa cells. They can live in a vial of nutrients, and from a small sample you can grow a large
Within this site you can view figures from the papers, supplementary data, and interactively explore the cell cycle dataset. In addition, raw data and processed data are available for download.. ...
In vitro knockdown of Bub1 in p53 impaired cells (e.g. HeLa cells) caused aneuploidy. Whether aneuploidy alone is a sufficient ... In humans Bub1 accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after ... Cell. 32 (3): 394-405. doi:10.1016/j.molcel.2008.09.017. PMC 2644263. PMID 18995837. UniProt: O43683 "BUB1 protein (Homo ... Jeganathan K, Malureanu L, Baker DJ, Abraham SC, van Deursen JM (Oct 2007). "Bub1 mediates cell death in response to chromosome ...
How HeLa Cells Work. HowStuffWorks. Retrieved 2016-03-17. How Underwater Tunnels Work. HowStuffWorks. Retrieved 2016-03-17. How ...
"A saga of HeLa cells". www.virology.ws. Information, Reed Business (9 June 1960). "New Scientist". Reed Business Information - ... "A Study by Fluorescence Microscopy of the Replication of Inclusion Blennorrhoea Virus in HeLa Cell Monolayers". Journal of ...
"Genetic transformation of HeLa cells by Agrobacterium". Proceedings of the National Academy of Sciences of the United States of ... By altering the hormone balance in the plant cell, the division of those cells cannot be controlled by the plant, and tumors ... The plasmid T-DNA is integrated semi-randomly into the genome of the host cell,[7] and the tumor morphology genes on the T-DNA ... This method transforms only cells in immediate contact with the bacteria, and results in transient expression of plasmid DNA.[ ...
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"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi: ... "Abrogation of signal-dependent activation of the cdk9/cyclin T2a complex in human RD rhabdomyosarcoma cells". Cell Death Differ ... Cell. 19 (4): 523-34. doi:10.1016/j.molcel.2005.06.027. PMID 16109376. Cottone G, Baldi A, Palescandolo E, Manente L, Penta R, ... Cell. Biol. 23 (14): 4859-69. doi:10.1128/MCB.23.14.4859-4869.2003. PMC 162212 . PMID 12832472. Beausoleil SA, Jedrychowski M, ...
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HeLa cells can be infected with SeV; however, incubation of these cells with IFN-beta causes inhibition of SeV replication. ... Not all cancer cells have cell entry receptors for the virus and not all cancer cells express virus processing serine proteases ... involves the fusion of infected cells with adjacent target cells and remains an important mechanism of direct cell-to-cell ... "Directed cell fusion" below). The virus can fuse up to 50-100 cells adjacent to one primary infected cell. This multi-nuclear ...
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... "Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis". Cell. 11 (2): ... This stimulates cell proliferation and gall formation. The T-DNA contains genes for encoding enzymes that cause the plant to ... The Ti plasmid integrates a segment of its DNA, known as T-DNA, into the chromosomal DNA of its host plant cells. A. ...
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... where plant cells of secondary tumours were found to lack any bacterial cells within. However, these tumour cells did possess ... Within the recipient cell, VirD2 will also work together with VirE2 to direct the transferred DNA to the recipient cell's ... These regions have features that allow the delivery of T-DNA into host plant cells, and can modify the host plant cell to cause ...
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... A cell culture that contains in its population cells with non-integrated F-plasmids usually also contains a few cells that have ... Conjugation diagram Donor cell produces pilus. Pilus attaches to recipient cell and brings the two cells together. The mobile ... Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi: ... "Blood Cells Mol. Dis. 39 (3): 336-9. doi:10.1016/j.bcmd.2007.06.009. PMC 2387274. PMID 17698380.. ... Cell. Proteomics. 7 (3): 499-508. doi:10.1074/mcp.M700325-MCP200. PMID 18029348.. ... 2009). "Meningioma 1 gene is differentially expressed in CD34 positive cells from bone marrow of patients with myelodysplastic ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi: ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ... cell nucleus. • cytoplasm. • nucleoplasm. • cytosol. Biological process. • protein K63-linked ubiquitination. • protein K48- ... Cell. Biol. 22 (4): 1266-75. doi:10.1128/MCB.22.4.1266-1275.2002. PMC 134630 . PMID 11809816.. ...
Joguchi A, Otsuka I, Minagawa S, Suzuki T, Fujii M, Ayusawa D (Apr 2002). "Overexpression of VDUP1 mRNA sensitizes HeLa cells ... negative regulation of cell division. • regulation of cell proliferation. • positive regulation of apoptotic process. • cell ... cell nucleus. Biological process. • cellular response to tumor cell. • response to estradiol. • negative regulation of ... 25-dihydorxyvitamin D3 inhibits tumor cell growth by blocking cell-cycle progression". Oncogene. 22 (26): 4035-46. doi:10.1038/ ...
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"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Rbm7 in epithelial cells plays a critical role in the development of fibrosis by regulating ncRNA decay and thereby the ... Molecular Cell. 74 (2): 254-267.e10. doi:10.1016/j.molcel.2019.01.033. PMC 6482433. PMID 30824372. Fukushima K, Satoh T, ... "Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the ...
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"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e. ...
August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of ... This reduced fertility appears to be due to germ cell attrition resulting from a combination of unrepaired DNA breaks during ... June 2006). "Control of BRCA2 cellular and clinical functions by a nuclear partner, PALB2". Molecular Cell. 22 (6): 719-29. doi ... June 2006). "Control of BRCA2 cellular and clinical functions by a nuclear partner, PALB2". Molecular Cell. 22 (6): 719-29. doi ...
... a change can be seen in HeLa cells with a TATAAAA to TATACAA which leads to a 20 fold decrease in transcription.[31] Some ... "Molecular Cell. 68 (1): 130-143.e5. doi:10.1016/j.molcel.2017.08.016. PMC 5632562. PMID 28918903.. ... In specific cell types or on specific promoters TBP can be replaced by one of several TBP-related factors (TRF1 in Drosophila, ... Compounds that trap the protein-DNA intermediate could result in it being toxic to the cell once they encounter a DNA ...
"Transcriptional regulation of squalene epoxidase by sterols and inhibitors in HeLa cells". The Journal of Biological Chemistry ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Cell. 154 (2): 452-64. doi:10.1016/j.cell.2013.06.022. PMC 3717207 . PMID 23870131. "Infection and Immunity Immunophenotyping ( ... "Identification of genes differentially expressed in human primary lung squamous cell carcinoma". Lung Cancer. 56 (3): 307-17. ...
This is done in modern cells by ribosomes, a complex of several RNA molecules known as rRNA together with many proteins. The ... In segmented RNA viruses, "mating" can occur when a host cell is infected by at least two virus particles. If these viruses ... For two, rather than one, viable daughter cells to be produced would require an extra replication of the intact RNA gene ... The existence, in extant cells, of RNAs with molecular properties predicted for RNAs of the RNA World constitutes an additional ...
... the cell line was contaminated and displaced by HeLa cells, and has now been identified as actually HeLa cells.[54] ... T-cells and B-cells) and antigen presenting cells. These cells coordinate an immune response upon the detection of foreign ... Hargraves M, Richmond H, Morton R. Presentation of two bone marrow components, the tart cell and the LE cell. Mayo Clin Proc ... Microscope slides are coated with HEp-2 cells and the serum is incubated with the cells. If the said and targeted antibodies ...
In the 1960s, virologist and cancer researcher Chester M. Southam injected HeLa cancer cells into patients at the Jewish ... or cells derived from females as well as males if they are studying cell cultures, and that the NIH would take the balance of ... Third, maintain a diverse research portfolio in order to capitalize on major discoveries in a variety of fields such as cell ... 1943 - Wilton R. Earle pioneered the cell culture process and published a paper describing the production of malignancy in ...
HeLa cells). But where 5-LOX was in active state, zileuton could effectively inhibit progression, as in case of prostate cancer ... lo Prostate Cancer Cell Population Harbors Self-Renewing Long-Term Tumor-Propagating Cells that Resist Castration". Cell Stem ... LNCaP cells express androgen receptor (AR), but PC-3 and DU-145 cells express very little or no AR. AR, an androgen-activated ... Prostate cancer cells are generally devoid of zinc. This allows prostate cancer cells to save energy not making citrate, and ...
Diamond (13 Mayıs 2020). "The challenges of vaccine development against a new virus during a pandemic". Cell Host and Microbe. ... HeLa hücrelerinde TMPRSS2'nin inhibe edilmesi, SARS-CoV ve insan koronavirüsü NL63 enfeksiyonlarının kısmen bloke edilmesine ... "Cell. 14 Mart 2020 tarihinde kaynağından arşivlendi. Erişim tarihi: 5 Mart 2020.. ... uses the SARS-coronavirus receptor ACE2 and the cellular protease TMPRSS2 for entry into target cells". bioRxiv (preprint). doi ...
All Central government offices and PSUs are to establish Hindi Cells for implementation of Hindi in their offices.[45] ... Hela Havula. *Nepal Bhasa movement. *Punjabi Language Movement. *Pure Tamil movement. *Sanskrit revival ...
Görlich, Dirk (1999). "Transport between the cell nucleus and the cytoplasm". Ann. Rev. Cell Dev. Biol. (15): 607-660. doi: ... HeLa ಸೆಲ್ಸ್ ಸ್ಟೇಯ್ನಡ್ ಫಾರ್ DNA ವಿಥ್ ದಿ ಬ್ಲೂ ಹೋಶ್ಟ್ ಡೈ. ಮಧ್ಯಂತರ ಮತ್ತು ಬಲತುದಿಯ ಜೀವಕಣವು ಇಂಟರ್‌ಫೇಸ್‌ನಲ್ಲಿರುತ್ತದೆ, ಆದುದರಿಂದ ಅವುಗಳ ... Görlich, Dirk; Kutay, U (1999). "Transport between the cell nucleus and the cytoplasm". Ann. Rev. Cell Dev. Biol. 15 (15): 607- ... Goldring, SR (1987). "Human giant cell tumors of bone identification and characterization of cell types". J Clin Invest (79(2 ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. PMC ... Cell. 125 (4): 801-14. PMID 16713569. doi:10.1016/j.cell.2006.03.032. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط ,الصفحات=. تم ... Cell. 18 (4): 1385-96. PMC 1838996. . PMID 17392519. doi:10.1091/mbc.E06-12-1120. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط , ... doi:10.1016/j.cell.2006.09.026. الوسيط ,السنة=. تم تجاهله (مساعدة); الوسيط ,الصفحات=. تم تجاهله (مساعدة); الوسيط ,العنوان=. تم ...
W. Martin, M. J. Russell: On the Origin of Cells: a Hypothesis for the Evolutionary Transitions from Abiotic Geochemistry to ... Typisch mitochondrial Nettwark (gröön) in twee Minschenzellen (HeLa-Zellen). Dat Mitochondrium (ok Mitochondrion, Mt. ... B. Alberts u. a.: Molecular Biology of the Cell; 20024; ISBN 0-8153-3218-1 ... Molecular Biology of the Cell, Garland Publishing Inc., New York, ISBN 0815332181 ...
... is required for toxin targeting into the cytosol of HeLa cells". J. Biol. Chem. 275 (47): 36869-75. PMID 10930402. doi:10.1074/ ... "The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma ... "Identification of murine T cells reactive with the bacterial superantigen Yersinia pseudotuberculosis-derived mitogen (YPM) and ... "Two mammalian cell internalization strategies used by pathogenic bacteria". Annu. Rev. Genet. 28: 395-422. PMID 7893133. doi ...
In 1953, HeLa cells were the first human cells successfully cloned[11] and demand for the HeLa cells quickly grew in the ... These cells proliferate abnormally rapidly, even compared to other cancer cells. Like many other cancer cells,[36] HeLa cells ... HeLa (/ˈheɪlɑː/; also Hela or hela) is an immortal cell line used in scientific research. It is the oldest and most commonly ... but all HeLa cells are descended from the same tumor cells removed from Lacks. The total number of HeLa cells that have been ...
AFM image of part of a Golgi apparatus isolated from HeLa cells ... cancer cells and normal cells based on a hardness of cells, and ... AFM can also be used to indent cells, to study how they regulate the stiffness or shape of the cell membrane or wall. ... ligands). Evans and Calderwood used single cell force microscopy to study cell adhesion forces, bond kinetics/dynamic bond ... and the properties of the cells has also given insight into chemical processes and mechanisms that occur through cell-cell ...
"Differential transcription of the orphan receptor RORbeta in nuclear extracts derived from Neuro2A and HeLa cells.". Nucleic ...
To explore the effects of garcinol on histone acetyltransferases, researchers used HeLa cells. The cells underwent irradiation ... doi:10.1016/j.cell.2006.03.033. PMID 16678094.. *^ a b c d e f Kimura A, Matsubara K, Horikoshi M (December 2005). "A decade of ... Cell Biol. 8 (4): 284-95. doi:10.1038/nrm2145. PMID 17380162.. *^ a b Weaver R (2007). Molecular Biology. McGraw-Hill. ISBN ... Ogryzko VV (May 2001). "Mammalian histone acetyltransferases and their complexes". Cell. Mol. Life Sci. 58 (5-6): 683-92. doi: ...
... and HeLa cells to determine substrate specificity. Plant ABCB transporters have shown to transport the phytohormone indole-3- ... In multidrug-resistant cells, the MDR1 gene is frequently amplified in multidrug-resistant cells. This results in a large ... The higher the MDR activity is in the cell membrane, the less Calcein is accumulated in the cytoplasm. In MDR-expressing cells ... In addition to conferring MDR in tumor cells, ABC transporters are also expressed in the membranes of healthy cells, where they ...
... along with Endoplasmic-reticulum-associated degradation in stressed HeLa cells. AB5 Toxins Biochemistry Cholera toxin Pertussis ... After their B subunit binds to receptors on the cell surface, the toxin is enveloped by the cell and transported inside either ... Once StxB targets a cancerous cell, it delivers the A subunit of the toxin which eventually kills the cancerous cell. Play ... SubAB's target is in the endoplasmic reticulum of the cell and is brought into the cell through clathrin-mediated endocytosis. ...
Statins also inhibit PPARγ in human macrophages, vascular endothelial cells, and smooth muscle cells; this action may ... Kühn, Hartmut; Barnett, Jim; Grunberger, Dorit; Baecker, Preston; Chow, Joan; Nguyen, Binh; Bursztyn-Pettegrew, Hela; Chan, ... and export of the 13-OXO-glutathione conjugate in HT-29 cells". Biochimica et Biophysica Acta (BBA) - Molecular and Cell ... this receptor is highly expressed in mouse and human airway epithelial cells and in Beas 2B human airway epithelial cells and, ...
HeLa cell[edit]. Apoptosis in HeLa[b] cells is inhibited by proteins produced by the cell; these inhibitory proteins target ... HeLa cells are an immortalized cancer cell line used frequently in research. The cell line was established by removing cells ... It can be interpreted by counting, measuring, and analyzing the cells of the Sub/G1 cell population.[100] When HeLA cells are ... cells that are cultivated in distinct and specific conditions. An example of this can be seen in HeLa cells, whereby the cells ...
"Genetic transformation of HeLa cells by Agrobacterium". Proc. Natl. Acad. Sci. U.S.A. 98 (4): 1871-6. doi:10.1073/pnas. ... Conjugation diagram 1- Donor cell produces pilus. 2- Pilus attaches to recipient cell and brings the two cells together. 3- The ... Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge ... These genes turn plant cells into factories producing chemicals used by the bacteria for nitrogen and energy. Infected cells ...
The migration ability of HeLa cells transfected with Cdc42 was higher than that of non-transfected cells.[9] It was proposed ... single organismal cell-cell adhesion. • cell differentiation. • positive regulation of epithelial cell proliferation involved ... Normal cancer cells and Cdc42-deficient cancer cells have also been compared in vivo. When both types of cells were injected ... cell projection. • myelin. • extracellular exosome. • filopodium. • plasma membrane. • spindle. • apical part of cell. • ...
"Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM ... Solubilization of cell membrane bilayers requires a detergent that can enter the inner membrane monolayer.[7] Advancements in ... grade detergents are employed for the isolation and purification of integral membrane proteins found in biological cells.[6] ...
... scientists used cat cells, mouse cells, cat and mouse hybrid cells, mink cells, dog cells, human cells, and HeLa cells.[10] ... Use of HeLa cells in parvovirus testing[edit]. Testing for how feline parvovirus and canine parvovirus infect cells and what ... Testing of HeLa cells and human cells to exposure of both feline parvovirus and canine parvovirus resulted in infections of the ... Transcription only initiates when the host cell enters S-phase under its own cell cycle control, at which time the cell's ...
... instead showing that the predominant complex including BRCA1 in HeLa cells is a 2 megadalton complex containing SWI/SNF.[51] ... Durant ST, Nickoloff JA (2005). "Good timing in the cell cycle for precise DNA repair by BRCA1". Cell Cycle. 4 (9): 1216-22. ... Cell Biol. 13 (11): 1376-82. doi:10.1038/ncb2367. PMID 22020440.. *^ Zhang H, Wang Q, Kajino K, Greene MI (2000). "VCP, a weak ... cell signaling, oxidative metabolism, and cellular transport". Exp. Cell Res. 289 (2): 211-21. doi:10.1016/S0014-4827(03)00261- ...
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi: ... "Mol Cell Biol. 12 (12): 5673-82. doi:10.1128/mcb.12.12.5673. PMC 360507. PMID 1448097.. الوسيط ,CitationClass=. تم تجاهله ( ... regulation of myeloid cell differentiation. • ضبط نسخ الدنا. • negative regulation of transcription from RNA polymerase II ... doi:10.1016/j.cell.2006.09.026. PMID 17081983.. الوسيط ,CitationClass=. تم تجاهله (مساعدة). ...
HeLa (nan); HeLa, תאי HeLa (he); HeLa-Zelle, HeLa-Zellen (de); He-la (pt); Células HeLa (gl); HeLa (ml); HELA, He La, He-La, ... HeLa (kk); HeLa (lfn); HeLa (cs); எலா உயிரணுக்கள் (ta); HeLa (it); HeLa (fr); HeLa (et); HeLa (pt); HeLa (gl); HeLa (uk); HeLa ... HeLa sè-pau (nan); ഹിലാ (ml); HeLa (nl); HeLa (eo); HeLa (pl); HeLa (ru); HeLa (nn); HeLa (en); خلايا هيلا (ar); HeLa (id); ... HeLa (es); HeLa (eu); HeLa (ca); HeLa (de); هلا (fa); 海拉细胞 (zh); HeLa (da); HeLa (tr); HeLa細胞 (ja); HeLa (sv); תאי הילה (he); 海 ...
Learn about the discovery and ethics of HeLa cells. ... HeLa cells have been star players in medical research for ... Under the right conditions, HeLa cells form an immortal cell line; they divide indefinitely. Remember that HeLa cells were ... But whats a HeLa cell? Its a line, or population, of cells, taken from a person and used in scientific research. Cell lines ... HeLa cells have even been sent to outer space, proving that cancer cells can grow there. Almost since its creation, the HeLa ...
These results were interpreted as reflecting widespread contamination of cultures by the first established human cell line HeLa ... These findings were presented at the Second Decennial Review Conference on Cell Tissue and Organ Culture4 and have since been ... and in view of the widespread use of cell lines in a variety of investigations it seems important to make these findings ... but polymorphic variants detectable at the cell culture level can be very useful for this purpose, A survey of twenty ...
HeLa Cell Lysate and Synthetic Phosphopeptide Library. Cell lysate was prepared by using unsynchronized HeLa cells lysed by ... HeLa Cell Nuclear Preparation, Preparative SDS/PAGE Separation, and in-Gel Proteolysis. HeLa cell nuclear preparation was as ... Cell Signaling Technology, Beverly, MA) for synthetic peptides, and S. Gerber (Harvard Medical School) for HeLa cell lysate. ... Large-scale characterization of HeLa cell nuclear phosphoproteins. Sean A. Beausoleil, Mark Jedrychowski, Daniel Schwartz, ...
HeLa line) cells that were transfected with a pEYFP-Tubulin plasmid subcellular localization vector. ... HeLa Cell Tubulin. YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set. Fluorescence emission intensity from a ... cells that were transfected with a pEYFP-Tubulin plasmid subcellular localization vector. Cells were transiently transfected ... culture of human cervical carcinoma epithelial (HeLa line) ...
Mathematical modeling of the heat-shock response in HeLa cells.. Scheff JD1, Stallings JD2, Reifman J3, Rakesh V1. ... Our model was also in agreement with experiments showing that the number of HSF molecules in a HeLa cell is roughly 100 times ... the literature to obtain a compendium of in vitro hyperthermia experiments investigating the heat-shock response in HeLa cells ...
Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately ... HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells ... HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa ... Cell lines and Lysates. Multiplex miRNA assays. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. ...
HeLa Cell Dying, SEM Photographic Print by Thomas Deerinck. Find art you love and shop high-quality art prints, photographs, ... phagocytosed engulfed and digested by specialist cells HeLa cells are a continuously cultured cell line of human cancer cells ... HeLa cell dying coloured scanning electron micrograph SEM This cell appears spherical because it is undergoing apoptosis or ... programmed cell death Apoptosis occurs when a cell becomes old or damaged Blebs vesicles called apoptotic bodies form on its ...
In this paper we address this topic by constant observation of nanoviscosity of HeLa cells cytoplasm during S, G2 and G1 phases ... Nanoviscosity is an outcome of variable chemical and structural factors, which can temporarily change with cell-cycle ... Thus, the question arises, whether rates of biochemical reactions depend on the point of cell cycle. ... a counter-intuitive stability of cytoplasmic viscosity was observed during the cell cycle. Our results hint at possible ...
... applications for access to the genetic sequence of cells derived from a tumor that killed Henrietta Lacks. The cells are among ... More than 70,000 published scientific papers mention these cells.. These HeLa cells are so important that several laboratories ... HeLa Cells: NIH And Lacks Family Agree On DNA Access : Shots - Health News A special committee that includes two members of the ... HeLa cells have been mentioned in more than 70,000 published scientific papers. Tom Deerinck/NCMIR hide caption ...
ZMYM3 KO Cell Line. Mutation description: Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2. KO ... Human Wild Type HeLa cell line (ab255448). Please note a wild type cell line is not automatically included with a KO cell line ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, supplemented with 10% (v/v) DMSO ...
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.. SignalSlide is a registered trademark of Cell ... isoforms correlates with cell type, ligand and cell maturation stage (8).. * Ihle, J.N. (2001) Curr Opin Cell Biol. 13, 211-7. ... Each control slide contains formalin fixed, paraffin-embedded HeLa cells, untreated, treated with Human Interferon-α1 (hIFN-α1 ... Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or IFNa-treated (right), using Phospho- ...
As a result, scientific studies about certain cells are actually discussing other cells. Willem Halffman and Serge Horbach, ... researchers at Radboud University, found more than 30,000 publications on the wrong cells. Scientific journal PLOS ONE will ... immortal cells such as the famous HeLa cells have been contaminating other cell cultures in the lab. ... For decades, immortal cells such as the famous HeLa cells have been contaminating other cell cultures in the lab. As a result, ...
... which significantly reduces the amount of time taken to analyse the cell line named after Henrietta Lacks, an African-American ... The HeLa cell line was developed in the 1950s from a particularly aggressive strain of cervical cancer cells taken during a ... cell and background of three hundred slices of a HeLa cell observed with electron microscopy. *The open source code for all the ... City, University of London academics develop algorithm to analyse HeLa cancer cells Dr Constantino Carlos Reyes-Aldasoro and Dr ...
... and a cell is used to obtain the impedance of a single HeLa cell. Simulations indicated that the circle and parallel electrodes ... Effect of Electrode Shape on Impedance of Single HeLa Cell: A COMSOL Simulation. Min-Haw Wang and Wen-Hao Chang ... Additionally, increasing the operating voltage reduces the impedance magnitude of a single HeLa cell in all electrode shapes. ... Decreasing impedance magnitude of the single HeLa cell increases measurement sensitivity, but higher operational voltage will ...
Top : Forum Archives: : Cell Biology. RNA in HeLa cell - amount of RNA in a HeLa cell (Jun/08/2007 ). Hey all,. Could anyone ... From a T75 flask of subconfluent HeLa cells, I extracted about: 3ug/ul. I used the TRI Reagent.. Hope this helps.. L ... Could anyone tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment. ... tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment.Thanks a lot! ...
Purification and characterization of two forms of DNA polymerase alpha from HeLa cell nuclei.. Enomoto T, Tanuma S, Yamada M. ...
Here is the blurb from Amazon: From a single, abbreviated life grew a seemingly immortal line of cells that made some of the ... human cells that could survive--even thrive--in the lab. Known as HeLa cells, their stunning potency gave scientists a building ... Perhaps we could find a reflective laboratory biologist who has used HeLa cells (or something similar) and would be willing to ... I have a great article on HeLa too -- by Hannah Landecker. I have the PDF if you want me to email it to you! Its a really ...
Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ... matter how you are using your cells, you can count on these products to help keep them healthy. ...
What is HeLa cell? Meaning of HeLa cell as a legal term. What does HeLa cell mean in law? ... Definition of HeLa cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. ... HeLa cell legal definition of HeLa cell https://legal-dictionary.thefreedictionary.com/HeLa+cell ... cell. (redirected from HeLa cell). Also found in: Dictionary, Thesaurus, Medical, Financial, Encyclopedia, Wikipedia. cell. ( ...
HeLa Cell Program: Exploring Microscopy and Cancer. By Tom Gralinski, STEM Coordinator. Smith College Jandon Center for ... 4 - Another group of students takes a look at the HeLa cells. ... as one of the students takes a closer look at the cancer cell ...
... Biochemistry. 1993 Apr 20;32(15):4083-9. ... we performed replication reactions using a HeLa cell extract and the SV40 large T antigen. Frameshifts that restore the reading ...
Shop a large selection of Cell Line Lysates products and learn more about Novus BiologicalsiNOS Knockout HeLa Cell Lysate. iNOS ... Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... The iNOS Knockout HeLa Cell Lysate is derived from human. It has been validated for the following applications: Western Blot. ... 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines. ...
Novus Biologicals PTGFRN Knockout HeLa Cell Lysate PTGFRN Knockout HeLa Cell Lysate,Quantity: 2 Vials Life Sciences:Protein ... Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Novus Biologicals PTGFRN Knockout HeLa Cell Lysate This product is for research use only and is not approved for use in humans ... The FPRP Knockout HeLa Cell Lysate is derived from human. It has been validated for the following applications: Western Blot. ...
... of a cultured Hela cell (centre) on MDCK kidney cells (background). HeLa cells are a continuously cultured cell line of human ... MDCK cells are a strain of cultured, normal kidney epithelial cells. - Stock Image C011/1346 ... Numerous microvilli (string-like) can be seen, which cancer cells use to enable them to move around and spread (metastasise) ... cancer cells, which are immortal and so thrive in the laboratory. ...
... human cervical cancer cell), showing the cytoskeletal microfilaments (actin, red), and nuclei stain with Hoechst (DNA, blue), ... Caption: Fluorescence micrograph of a HeLa cell (human cervical cancer cell), showing the cytoskeletal microfilaments (actin, ... Keywords: actin, black background, blue, cancer, cell, cervical, color image, cytoskeleton, dna, fluorescence, hela, hoechst, ...
EXOC2 KO cell line available now. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp ... Wild-type HeLa cell lysate. Lane 2 : EXOC2 knockout HeLa cell lysate. Lane 3 : SH-SY5Y cell lysate. Lane 4 : Daudi cell lysate ... Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x104 cells/cm2. Seeding density is given ... Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell ...
... have been made as attractive molecular probes for labelling cells in biological research. In this study high-quality CdSe QDs ... Hela Cells. Humans. Immunoassay / methods*. Molecular Probe Techniques*. Quantum Dots*. Selenium Compounds. ... 972176 - Ultrastructural and enzymic modulation of hela cells induced by sodium butyrate and the.... 9788816 - Protein kinase c ... a cancer marker expressed on the surface of HeLa cells. The primary antibody (Ab) (rabbit anti-CEA8) and secondary Ab (goat ...
The electrical properties of single cells provide fundamental insights into their pathological condition and are therefore of ...
  • Cell lines are often named after the people from whom they were originally derived, and HeLa comes from the first two letters in the name Henrietta Lacks. (howstuffworks.com)
  • Henrietta Lacks had no idea that her cells were taken and used in this way, and neither did her family. (howstuffworks.com)
  • The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. (abcam.com)
  • A special committee that includes two members of the Lacks family will review scientists' applications for access to the genetic sequence of cells derived from a tumor that killed Henrietta Lacks. (npr.org)
  • The family of the late Henrietta Lacks finally got the chance to weigh in on how scientists use cells taken from her - without consent - more than 60 years ago. (npr.org)
  • The same was true back in 1951 when Henrietta Lacks unwittingly made available to scientists one of the most useful tools in research: cells that replicate and grew indefinitely in the lab. (npr.org)
  • It was established in 1951 from a biopsy taken from Henrietta Lacks and was the first human cell line to survive and grow in the laboratory. (wellcomecollection.org)
  • HeLa was named after Henrietta Lacks, the woman whose cervical cancer cells were biopsied and used for medical research. (eurekalert.org)
  • HeLa cells were thrust into the public eye in recent years thanks to the book and the made-for-TV film of the same name The Immortal Life of Henrietta Lacks. (eurekalert.org)
  • The HeLa cell line was developed in the 1950s from a particularly aggressive strain of cervical cancer cells taken during a routine biopsy from a 30-year-old African-American mother of five named Henrietta Lacks. (eurekalert.org)
  • And from that same life, and those cells, Rebecca Skloot has fashioned in The Immortal Life of Henrietta Lacks a fascinating and moving story of medicine and family, of how life is sustained in laboratories and in memory. (hastac.org)
  • They were derived from cervical cancer cells taken in 1951 from Henrietta Lacks, a patient who later died from the disease. (moleculardevices.com)
  • The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. (umassmed.edu)
  • The cells were taken from Henrietta Lacks, an African American woman who died of cervical cancer in 1951. (tanzforumberlin.de)
  • This cell line can betraced back to a woman named Henrietta Lacks, who died of cervical cancer in the early 1950s. (patentgenius.com)
  • Health Editor's Note: Back in 1951, cancer cells from a black woman, Henrietta Lacks, began the HeLa cell line which supports a multibillion-dollar biotechnology industry. (veteranstoday.com)
  • In The Immortal Life of Henrietta Lacks by Rebecca Skloot, according to Defler, why was the discovery of HeLa cells important? (enotes.com)
  • Rebecca Skloot describes in her book, The Immortal Life of Henrietta Lacks , how a lab mix up in Texas in 1953 accidentally enabled researchers to see and count each chromosome clearly in the HeLa cells they were working with. (technologynetworks.com)
  • Based on transformed epithelial cells derived from a cervical cancer tumor biopsy of a patient named Henrietta Lacks, HeLa was the first human cell line established in culture. (ku.dk)
  • The cell line originates from a cervical cancer tumor of a patient named Henrietta Lacks, who later died of her cancer in 1951 ( Skloot 2010 ). (g3journal.org)
  • The line is derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951. (wikipedia.org)
  • By using a strategy based on strong cation exchange chromatography, phosphopeptides were enriched from the nuclear fraction of HeLa cell lysate. (pnas.org)
  • The iNOS Knockout HeLa Cell Lysate is derived from human. (fishersci.com)
  • You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. (novusbio.com)
  • Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies. (novusbio.com)
  • The lysate was prepared by first washing the cells in PBS. (antikoerper-online.de)
  • This whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. (acris-antibodies.com)
  • Fluorescence emission intensity from a culture of human cervical carcinoma epithelial ( HeLa line) cells that were transfected with a pEYFP-Tubulin plasmid subcellular localization vector. (microscopyu.com)
  • HeLa cells are human epithelial cells from a fatal cervical carcinoma. (abcam.com)
  • HeLa cells are an immortal human epithelial cell line derived from a cancerous tumour of the cervix (adenocarcinoma). (wellcomecollection.org)
  • Since we had determined that TLR4 was present in cervical tissues and could therefore mediate inflammatory signalling in the cervical mucosa, we next investigated the impact of LPS stimulation on HIV receptor expression in cervical epithelial cells, using HeLa cells as a model system. (thefreedictionary.com)
  • MDCK cells are a strain of cultured, normal kidney epithelial cells. (sciencephoto.com)
  • Enteropathogenic Escherichia coli markedly decreases the resting membrane potential of Caco-2 and HeLa human epithelial cells. (harvard.edu)
  • Abstract: 'In this study [investigators] used the whole-cell, current clamp variant of the patch clamp technique to demonstrate that EPEC infection of HeLa and Caco-2 human epithelial cells reduces cell resting membrane potential. (harvard.edu)
  • P.4822 left column 2nd paragraph: 'EPEC [Enteropathogenic Escherichia coli] depolarizes HeLa and Caco-2 [human epithelial cells] cell membranes. (harvard.edu)
  • Keratins are cytoskeletal proteins that are the major components of intermediate filaments in epithelial cells however, their role in bacterial infection has been disregarded. (frontiersin.org)
  • We sought to identify genes involved in the control of adherence of EHEC O157:H7 to cultured epithelial cells. (asm.org)
  • EHEC O157:H7 also harbor a large pathogenicity island, termed the locus for enterocyte effacement (LEE), which is associated with the intimate adherence to epithelial cells, initiation of host signal transduction pathways, and the formation of attaching-and-effacing intestinal lesions (reviewed in references 16 and 40 ). (asm.org)
  • These data and previous reports led us to hypothesize that other unidentified factors are involved in the adhesion to epithelial cells, and their functions have been difficult to establish because their expression is tightly regulated during conditions of in vitro adherence. (asm.org)
  • To investigate IAV tropism in human cells, we evaluated the replication of IAV strains in a diverse subset of epithelial cell lines. (asm.org)
  • Coloured scanning electron micrograph (SEM) of a cultured Hela cell (centre) on MDCK kidney cells (background). (sciencephoto.com)
  • Coloured scanning electron micrograph (SEM) of HeLa cells undergoing cytokinesis (cell division). (fineartamerica.com)
  • Six recently synthesized cyano-substituted heteroaryles, which do not bind to DNA but are highly cytotoxic against the human tumor cell line HeLa, were analyzed for their antitumor mechanisms of action (MOA). (springer.com)
  • A computational analysis was performed that compared the antiproliferative activities of our compounds in 13 different tumor cell lines with those of compounds listed in the National Cancer Institute database. (springer.com)
  • Furthermore, a second in silico investigation revealed that the tumor cells that are sensitive to the cyano-substituted compounds show differences in their expression of locomotion genes compared with that of insensitive cell lines, thus corroborating the involvement of the cytoskeleton. (springer.com)
  • Background: Fusion between malignant and primary, normal human cells results in the suppression of malignancy as measured by tumor formation of cells when inoculated into nude mice. (aacrjournals.org)
  • Induction of apoptosis in tumor cells has become the major focus of anti-tumor therapeutics development. (sigmaaldrich.com)
  • In the months before her death on October 4, 1951, cells derived from her tumor had been successfully cultured in the Hopkins laboratory of Dr. George Gey. (scienceblogs.com)
  • We measured temporal global gene expression in HeLa Hep2 tumor cells using bead chip arrays (Illumina) following exposure to 5 Gy of ionizing radiation (60Co). (diva-portal.org)
  • Therapeutic strategies employing these alterations might potentiate future therapy and enhance tumor cell killing. (diva-portal.org)
  • Radiation therapy uses ionizing radiation to induce DNA damage in tumor cells in order to kill them. (diva-portal.org)
  • Tumor cells are more sensitive than normal cells, since they have an increased proliferation rate and often lack the ability to properly repair the induced damage. (diva-portal.org)
  • During treatment against the tumor, the cells were extracted and passed on to a laboratory without her knowledge. (tanzforumberlin.de)
  • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)
  • Smart multifunctional drug delivery systems (DDSs) based on cytophilic fluorescent polyurethane copolymer microcapsules with high tumor cell internalization, triggered release, quick cancer cell death and real time fluorescent monitoring abilities is developed as a facile and versatile approach for precision cancer therapy. (rsc.org)
  • The cells from Lacks's cancerous cervical tumor were taken without her knowledge or consent, which was common practice at the time. (wikipedia.org)
  • Cells from Lacks's tumor behaved differently. (wikipedia.org)
  • There are many strains of HeLa cells as they continue to mutate in cell cultures, but all HeLa cells are descended from the same tumor cells removed from Lacks. (wikipedia.org)
  • The cell cycle allows growth and proliferation of cells, development of tissues and whole organisms. (nature.com)
  • As evidenced by MTT assay, GNPs inhibit proliferation of HeLa cells in dose-dependent GNPs and cytotoxicity of GNPs in Bone Marrow Mesenchymal Stem Cell (BMSCs) was lower than cancerous cells. (thefreedictionary.com)
  • Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. (novusbio.com)
  • The proliferation inhibition of juglone on HeLa cells was tested by the MTT assay. (sigmaaldrich.com)
  • Here, we describe the synthesis of procyanidin B 1 acetylated analogs and discuss their inhibition activities against HeLa S3 cell proliferation. (mdpi.com)
  • Surprisingly, the lower-unit acetylated procyanidin B 1 strongly inhibited the proliferation of HeLa S3 cells. (mdpi.com)
  • This result suggests that the phenolic hydroxyl groups of the upper-units in flavan-3-ols are important for their inhibitory activity against cancer cell proliferation and that a hydrophobic lower unit dimer enhances this activity. (mdpi.com)
  • Inhibition of HeLa cancer cell proliferation by bacteria was evaluated by MTT assay. (magiran.com)
  • However, it has become clear that ROS delicately regulate many cellular functions such as gene expression, differentiation and cell proliferation ( 2 ). (spandidos-publications.com)
  • Nevertheless, in both cases, H 2 O 2 may amend essential cellular functions of cell growth, proliferation and differentiation via altering signaling cascades and gene expression, or its higher level may lead to outcomes such as apoptosis or necrosis. (spandidos-publications.com)
  • It's a line , or population, of cells, taken from a person and used in scientific research. (howstuffworks.com)
  • But HeLa cells were the first -- the first line of human cells to survive in vitro (in a test tube). (howstuffworks.com)
  • It's the most commonly used cell line, and it's known to be extremely resilient. (howstuffworks.com)
  • He named the line HeLa. (howstuffworks.com)
  • These results were interpreted as reflecting widespread contamination of cultures by the first established human cell line HeLa. (nature.com)
  • Those cells were found to reproduce indefinitely in the lab, making them the world's first immortalised cell line and one of the most important and commonly used cell lines in medical research to this day. (eurekalert.org)
  • One solution would be to put a disclaimer on all 30,000 publications explaining that they report on the wrong cell line. (eurekalert.org)
  • The City/Francis Crick Institute team prepared and observed the HeLa cell line using Electron Microscopy (EM), which can acquire tens of thousands of data sets that can easily exceed several gigabytes of data per month. (eurekalert.org)
  • From a single, abbreviated life grew a seemingly immortal line of cells that made some of the most crucial innovations in modern science possible. (hastac.org)
  • After application of five different iminothiazolidinone derivatives (Compound A-E) to HeLa cell line for 24, 48 and 72 h, antimitotic effects of all treatments were evaluated with mitotic index analysis. (thefreedictionary.com)
  • However, the obtained results of the cytotoxicity testing of methanol extracts on Hela cell line unraveled that the highest cytotoxicity relates to Anabaena 55 at the dose of 30 mg / mL. (thefreedictionary.com)
  • these 3 three findings were thought sufficient to define a cell line as HeLa. (thefreedictionary.com)
  • DNA extracted from a SiHa cell line was used as the positive control for HPV16, whereas DNA extracted from a HeLa cell line was used as the positive control for consensus primers and for HPV18-specific primers. (thefreedictionary.com)
  • HeLa cells are a continuously cultured cell line of human cancer cells, which are immortal and so thrive in the laboratory. (sciencephoto.com)
  • These cells were called HeLa, so named for the first two letters of Henrietta Lacks's names, and took a place in history as the first human cell line to be continually propagated in culture. (scienceblogs.com)
  • The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line. (sigmaaldrich.com)
  • Efficiently upconverting, spherical ZnAl 2 O 4 nanoparticles (NPs), doped with erbium and ytterbium, were synthesized by a combustion aerosol method (CAM) and transported to cytosol of carcinoma cell line (HeLa) for the first time. (rsc.org)
  • The project confronts the history of the HeLa cells, the first human cell line to be cultivated in a laboratory. (tanzforumberlin.de)
  • Scientists were able to grow the first human cell line out of them, and later made numerous medical discoveries such as polio vaccination, as well as advances in cancer and HIV/ AIDS research. (tanzforumberlin.de)
  • The cell line HeLa is transformed with the chromosomal insertion of the plasmid pSV.sub.2 neoNR10.sub.1, ATCC No. 69617. (patentgenius.com)
  • 1. The cell line HeLaNR1. (patentgenius.com)
  • No. 5,464,768,"Enhanced nitrite production in transfected murine cells", issued Nov. 7, 1995 to Kiel et al, describes mammalian cells line capable of enhanced nitrite production prepared by transfecting a murine macrophage or murinethymoma with barley nitrate reductase gene (NR). The cell lines can be used for the production of diazomelanin (DM) and diazoluminomelanin (DALM). (patentgenius.com)
  • We then successfully modified EMT-6 cells (spontaneous Balb/c mammary adenocarcinoma cell line) with the chromosomal insertion of the plasmid pSV.sub.2 neoNR10.sub.1, ATCC No. 69617. (patentgenius.com)
  • We have now transfected a human cell line, HeLa, with a barley nitrate reductase gene fragment. (patentgenius.com)
  • Now descendants of her family will begin to receive financial compensation for the use of the HeLa cell line which have been used in research. (veteranstoday.com)
  • The HeLa line of cells were the first human cell line that reproduced successfully outside the body and thus enabled scientists to do breakthrough research especially in the area of studying cancer. (enotes.com)
  • These cells went on to become the immortal cell line known as HeLa. (technologynetworks.com)
  • The world's most famous human cell line has had its complete set of expressed proteins, its proteome, determined by mass spectrometry-based proteomics (ref. (ku.dk)
  • HeLa cells are by far the most widely-used cell line in biomedical research and it contributed significantly to the development of the vaccine against the polio virus. (ku.dk)
  • Rhododendron arboreum, traditionally used as a folk remedy by the people of North-East India was investigated for its effects on apoptotic induction and cell cycle arrest in Human Cervical Cancer (HeLa) cell line. (omicsonline.org)
  • Interestingly, a human isolate of the highly pathogenic avian influenza (HPAI) virus H5N1 successfully propagated in HeLa cells to levels comparable to a human lung cell line. (asm.org)
  • Those cells would go on to become the first immortal line of human cells, something of enormous benefit to science and humanity as a whole. (iheart.com)
  • But while the line, called HeLa cells, became a multi-billion-dollar industry, her family languished without health care insurance. (iheart.com)
  • HeLa is the most widely used model cell line for studying human cellular and molecular biology. (g3journal.org)
  • To date, no genomic reference for this cell line has been released, and experiments have relied on the human reference genome. (g3journal.org)
  • Here we present a detailed genomic and transcriptomic characterization of a HeLa cell line. (g3journal.org)
  • We performed DNA and RNA sequencing of a HeLa Kyoto cell line and analyzed its mutational portfolio and gene expression profile. (g3journal.org)
  • and has since become the most widely used human cell line in biological research. (g3journal.org)
  • also Hela or hela) is an immortal cell line used in scientific research. (wikipedia.org)
  • It is the oldest and most commonly used human cell line. (wikipedia.org)
  • The cell line was found to be remarkably durable and prolific, which allows it to be used extensively in scientific study. (wikipedia.org)
  • Cell biologist George Otto Gey found that they could be kept alive, and developed a cell line. (wikipedia.org)
  • this became the name of the cell line. (wikipedia.org)
  • This was the first human cell line to prove successful in vitro, which was a scientific achievement with profound future benefit to medical research. (wikipedia.org)
  • The cell line was named "HeLa" after the first two letters in Lacks's name. (wikipedia.org)
  • Lacks was not credited as the origin of the cell line until her real name leaked to the public in the 1970s. (wikipedia.org)
  • Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. (abcam.com)
  • Entry into HeLa cells required actin polymerization and an intact microtubule cytoskeleton and was only moderately inhibited by the presence of 100 mM glucose or galactose. (nih.gov)
  • In particular, pathogens usurp the actin cytoskeleton function to strongly adhere to the host cell surface, to induce plasma membrane remodeling allowing invasion and to spread from cell to cell and disseminate to the whole organism. (frontiersin.org)
  • They did not interfere with the expression of human papillomavirus oncogenes integrated in the HeLa cell genome, but they did induce strong G1 arrest and result in the activation of caspase-3 and apoptosis. (springer.com)
  • Last March, German scientists published the HeLa genome - again without consent from Henrietta's family - and controversy erupted . (npr.org)
  • The NIH then set up a committee that included the Lacks family to figure out a way to publish the HeLa genome, while still respecting the family's privacy. (npr.org)
  • This is article VII in the series Expression of the Mitochondrial Genome in HeLa Cells. (caltech.edu)
  • A portion of the HeLa genome has also been published without familial consent. (labroots.com)
  • Our results provide the first detailed account of genomic variants in the HeLa genome, yielding insight into their impact on gene expression and cellular function as well as their origins. (g3journal.org)
  • Chronically infected cultures of HeLa cells were also established that may be a useful tool for studying long-term interactions between virulent L. pneumophila and mammalian cells. (nih.gov)
  • One of the earliest variants, known as enhanced green fluorescence protein ( EGFP ), contains codon substitutions (commonly referred to as the S65T mutation) that alleviates the temperature sensitivity and increases the efficiency of GFP expression in mammalian cells. (fsu.edu)
  • Cherepanova NA, Gilmore R. Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation. (umassmed.edu)
  • This baculovirus was expected to express EBO NP under the control of the CMV-IE promoter in mammalian cells upon abortive infection with the virus ( 5 , 17 ). (asm.org)
  • These experiments firmly established the capability of this proteomics technology to enable comprehensively analysis of any mammalian cell or tissue proteome in a quantitative manner with sufficient throughput for clinical applications with larger patient cohorts. (ku.dk)
  • These reviews covered methods used for the infection of cells by viral nucleic acids as well as interaction of mammalian cells with non-viral nucleic acids. (springer.com)
  • Uptake of nonviral nucleic acids by mammalian cells. (springer.com)
  • A streamlined workflow for cell counting and phenotypic characterization is critical to many experiments. (moleculardevices.com)
  • Despite decades of tremendous developments in the proteomics technologies, complete proteome characterization of human cells have remained a formidable task. (ku.dk)
  • In this study, we have applied FLIM to characterize the metabolic changes in HeLa cells upon bacterial infection and made comparison with the results from the cells treated with staurosporine (STS), a well-known apoptosis inducer. (spiedigitallibrary.org)
  • Being associated with mitochondria, FAD lifetimes and redox ratio could indicate heterogeneous mitochondrial function, microenvironment with bacterial infection, and further pathway to cell death. (spiedigitallibrary.org)
  • The redox ratios for both EHEC-infected and STS-treated HeLa cells have been observed and these observations also indicate possible apoptosis induced by bacterial infection. (spiedigitallibrary.org)
  • HeLa cells were infected with AcCMV-EBO-NP at a multiplicity of infection of 20 PFU/cell. (asm.org)
  • Viral determinants in H5N1 influenza A virus enable productive infection of HeLa cells. (asm.org)
  • Competence of suspended HeLa Cells for infection by inactivated poliovirus particles and by isolated viral RNA. (springer.com)
  • The results showed that juglone inhibits the growth of HeLa cells in dose-dependent manner. (sigmaaldrich.com)
  • Based on MTT assays, H2O2 inhibited the growth of HeLa cells with an IC50 value of ~75 µM at 24 h. (spandidos-publications.com)
  • In conclusion, H2O2 inhibited the growth of HeLa cells via apoptosis and/or necrosis, which was accompanied by intracellular increases in ROS levels and GSH depletion. (spandidos-publications.com)
  • Under UV light irradiation, the TiO 2 nanofibers, particularly the mixed phase nanofibers, displayed much higher cell-killing efficiency than Pirarubicin (THP), which is a common drug to induce the apoptosis of HeLa cells. (springer.com)
  • HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro. (abcam.com)
  • It is expected, that aphidicolin inhibition does not affect DNA repair nor biosynthesis of RNA, and synchronized cells remain in physiological state and restart DNA replication shortly after removal of the compound 19 . (nature.com)
  • This MOA was also confirmed experimentally: the cyano-substituted heteroaryles disrupted the actin and the tubulin networks in HeLa cells and inhibited cellular migration. (springer.com)
  • Fluorescence micrograph of a HeLa cell (human cervical cancer cell), showing the cytoskeletal microfilaments (actin, red), and nuclei stain with Hoechst (DNA, blue), X1500 (at 13 x 17 in. (sciencephoto.com)
  • In addition, the cells were labeled for filamentous actin with Alexa Fluor 568 conjugated to phalloidin, and for nuclear DNA with the ultraviolet-absorbing probe DAPI. (fsu.edu)
  • Multiphoton fluorescence image of HeLa cells stained with the actin binding toxin phalloidin (red), microtubules (cyan) and cell nuclei (blue). (technologynetworks.com)
  • An equivalent circuit model incorporating the PBS solution, a pair of electrodes, and a cell is used to obtain the impedance of a single HeLa cell. (hindawi.com)
  • Additionally, increasing the operating voltage reduces the impedance magnitude of a single HeLa cell in all electrode shapes. (hindawi.com)
  • Decreasing impedance magnitude of the single HeLa cell increases measurement sensitivity, but higher operational voltage will damage single HeLa cell. (hindawi.com)
  • Kemnitzer W, Kuemmerle J, Jiang S, Sirisoma N, Kasibhatla S, Crogan-Grundy C, Tseng B, Drewe J, Cai SX (2009) Discovery of 1-benzoyl-3-cyanopyrrolo[1, 2-a]quinolines as a new series of apoptosis inducers using a cell- and caspase-based high-throughput screening assay. (springer.com)
  • MTT assay and flow cytometry (FCM) were carried out to evaluate the biocompatibility, percentage of apoptotic cells, and cell viability. (springer.com)
  • Calculating cell confluence can also be helpful for assay development. (moleculardevices.com)
  • For example, you can assay your cells at several different densities and determine which one yields the best assay results. (moleculardevices.com)
  • MTT assay was used to evaluate cell viability and cytotoxic effect of aqueous-methanol extract of R. arboreum was determined. (omicsonline.org)
  • There is ever growing number of evidence supporting thesis, that deregulation of cell cycle machinery can induce disastrous changes in organism. (nature.com)
  • The cell cycle has been extensively studied for years which led to greater understanding of life of a cell and organism. (nature.com)
  • All of the body's normal cells experience the effects of aging over time, known as cellular senescence . (howstuffworks.com)
  • This is called programmed cell death ( PCD ), apoptosis or even cellular suicide . (howstuffworks.com)
  • The cell cycle is a universal process during which, through a series of cellular events, single cell gives rise to two daughter individuals. (nature.com)
  • At the individual cell level, the electrical properties of the cell are helpful for understanding the effects of cellular behavior. (hindawi.com)
  • Decreased expression of unknown genes in tumorigenic compared to non-tumorigenic cells, including EFEMP2, a cellular gene located on chromosome 11q13. (aacrjournals.org)
  • Macroautophagy (called just autophagy hereafter) is an intracellular degradation machinery essential for cell survival under stress conditions and for the maintenance of cellular homeostasis. (csic.es)
  • During a lecture, Defler talked about how biologists had spent many years trying to make human cells reproduce outside the body so that it would be possible to study human cellular biology in the laboratory. (enotes.com)
  • These findings suggest that the increased NADH fluorescence lifetime in STS-induced cell death occurred before the depletion of ΔΨ and ATP and activation of caspase 3, and was not simply caused by cellular metabolic change. (spiedigitallibrary.org)
  • Overall, this study suggests the absence of one or more cellular factors in HeLa cells that results in abortive replication of H1N1, H3N2, and LPAI viruses, but can be circumvented upon introduction of H5N1 NP and HA. (asm.org)
  • This study is also very similar to our method of study in that the methanol and aqueous extracts of the samples which were investigated in cytotoxicity way revealed that among the samples, Anabaena ISC 90 and Anabaena ISC 55 samples of the methanol extract and Anabaena 88 sample of the aqueous extract had a greater impact on HeLa cell lines. (thefreedictionary.com)
  • Bottom row: HeLa cells were treated with low (left) or high (right) concentrations of staurosporine and assayed for cytotoxicity with the EarlyTox Cell Integrity Kit. (moleculardevices.com)
  • Differently charged coatings demonstrated dissimilar behavior in terms of agglomeration in media, serum protein adsorption, nanoparticle cytotoxicity and cell internalization. (wiley.com)
  • Compound was tested for its cytotoxicity against HeLa epithelioid cervix carcinoma cells. (nih.gov)
  • While using very effective anti-cancers such as Doxorubicin and cisplatin, cells overcome the cytotoxicity of these drugs through promotion of what so-called cytoprotective autophagy. (medworm.com)
  • Oxidative stress-induced cytotoxicity in cervical cancer cells may be of toxicological interest. (spandidos-publications.com)
  • Receptor activation promotes tyrosine phosphorylation of Stat proteins, resulting in Stat dimerization and translocation to the nucleus where they regulate expression of numerous proteins that control cell growth, survival, differentiation and pathogen resistance (1). (cellsignal.com)
  • The blood-brain barrier is a collection of specialized cells and proteins that control the movement of molecules from the blood to the central nervous system. (the-scientist.com)
  • I lyse my cells, I quantify my proteins and load around 50ug sample on 8% gel and after transfer I stain my nitrocellulose membrane with Ponceau. (protocol-online.org)
  • All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins. (antikoerper-online.de)
  • Western blot analysis of apoptotic related proteins in HeLa cells treated with alsterpaullone. (hindawi.com)
  • Cells were also exposed to 0.3% DMSO and showed stable basal levels of the various proteins at the different time points. (hindawi.com)
  • We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
  • These cells are a convenient tool to determine the infl uence of the downregulation or overexpression of specifi c proteins in the autophagic fl ux as well as the analysis of autophagy-modulating compounds. (csic.es)
  • Study of the gene expression of proteins involved in apoptosis and cell cycle regulation was carried out using real time PCR technique. (omicsonline.org)
  • There was significant down-regulation in the gene expression of G0/G1 phase-related proteins CDK 1, cyclin B1, CDK 4 and cyclin D1 in comparison to untreated HeLa cells, inferring an inhibitory action of the plant extract on the cyclin dependent kinases and activation of cell cycle checkpoints. (omicsonline.org)
  • Here, we employ stable isotope labeling with amino acids in cell culture (SILAC)-based proteomics to quantify the response of over 6200 proteins to increasing concentrations of tunicamycin in HeLa cells. (biologists.org)
  • Unexpectedly, there are few strongly downregulated proteins, despite expression of the pro-apoptotic transcription factor CHOP, suggesting that IRE1-dependent mRNA decay (RIDD) has a limited contribution to ER stress-mediated cell death in our system. (biologists.org)
  • The modified cells can be used to study mechanisms for radiofrequency and light radiation interactions with carcinoma of the cervix. (patentgenius.com)
  • The effects of drugs, hormones, and cytokines that affect the expression of nitric oxide synthase and its activity can also be studied to understand the effects of these materials on cervix cells. (patentgenius.com)
  • These cells are treated as cancer cells, as they are descended from a biopsy taken from a visible lesion on the cervix as part of Lacks's diagnosis of cancer. (wikipedia.org)
  • Fluorescence lifetime imaging microscopy (FLIM) is a sensitive technique in monitoring functional and conformational states of nicotinamide adenine dinucleotide reduced (NADH) and flavin adenine dinucleotide (FAD),main compounds participating in oxidative phosphorylation in cells. (spiedigitallibrary.org)
  • The HeLa carcinoma cell culture featured in the digital image above was transfected with an EGFP-peroxisomal targeting signal 1 (PTS1) fusion protein and stained with MitoTracker Red CMXRos. (fsu.edu)
  • Accordingly, it is an object of the present invention to provide a human carcinoma cell modified with a nitrate reductase gene fragment. (patentgenius.com)
  • Ready-to-use whole cell lysates produced are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. (antikoerper-online.de)
  • Part of the team's research requires the identification of the nuclei of these cells, which is a complicated task that can take an expert around a week to accomplish. (eurekalert.org)
  • So, in the presence of serum, anionic liposomes effectively transport precondensed ON into the cell nuclei of HeLa cells in the present work. (thefreedictionary.com)
  • only the nuclei of the drug-sensitive cells incorporated the tritium. (sciencemag.org)
  • For decades, immortal cells such as the famous HeLa cells have been contaminating other cell cultures in the lab. (eurekalert.org)
  • Unfortunately, HeLa cells have been contaminating other cell cultures for decades. (eurekalert.org)
  • Due to, for instance, carelessness in the lab these rapidly reproducing cells began taking over other cell cultures. (eurekalert.org)
  • Large amounts of cell cultures have therefore been mislabelled. (eurekalert.org)
  • Various initiatives have been developed over the years to prevent the exchange of cell cultures, such as better protocols and hygienic fume cupboards. (eurekalert.org)
  • When investigated with double whole-cell patch-clamp measurements, HeLa cells in monolayer cultures were electrically coupled in 39% of the cases with very low transjunctional conductances (average one to five open channels). (uni-stuttgart.de)
  • A comparison was made of the polyamine content and S-phase DNA synthesis in cells from control cultures and cultures to which an inhibitor of polyamine biosynthesis, alpha-difluoromethylornithine, was added to the synchronization medium. (biochemj.org)
  • Almost complete restoration of DNA synthesis in cells depleted of polyamines was achieved by the addition of a polyamine to cultures at least 10 h before release of the second thymidine block. (biochemj.org)
  • Incubation of HeLa cells with the supernatant from preinduced EPEC cultures failed to elicit a drop in RMP relative to control values. (harvard.edu)
  • A HeLa cell culture production laboratory was set up at Tuskegee University, which at its peak was shipping in the region of 20,000 tube cultures per week. (technologynetworks.com)
  • After Gartler's discovery that HeLa cells could travel through the air and contaminate other cultures, vast improvements were made to cell culture practices, to prevent further costly cross-contaminations. (technologynetworks.com)
  • Perhaps we could find a reflective laboratory biologist who has used HeLa cells (or something similar) and would be willing to talk about the intellectual and property rights associated with knowledge derived from human specimens. (hastac.org)
  • 1 Laboratory of Molecular and Cell Genetics, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China. (nih.gov)
  • Those unique and important cells were the first to be cultured and reproduced in a laboratory. (labroots.com)
  • She has strong domain knowledge with experiences in a wide range of laboratory techniques including enzymology, molecular biology, bioassay development, cell culture and handling of modern advanced scientific instruments. (omicsonline.org)
  • HeLa cells, like other cell lines, are termed "immortal" in that they can divide an unlimited number of times in a laboratory cell culture plate as long as fundamental cell survival conditions are met (i.e. being maintained and sustained in a suitable environment). (wikipedia.org)
  • The cell cycle of somatic cells is commonly divided into two periods: interphase and mitosis. (nature.com)
  • It was expected, that significant structural changes in cells during biosynthesis or mitosis would have an impact on nanoviscosity, and further - diffusive motion of biomolecules. (nature.com)
  • During anaphase (one of the stages of nuclear division in mitosis), the spindle pulls the condensed chromosomes to opposite poles of the cell. (wellcomecollection.org)
  • This arrest was transient and cells entered mitosis before DNA damage was repaired causing anaphase bridge formation. (diva-portal.org)
  • P.660 left column top paragraph: 'Nup96 Levels Regulate Cell Cycle Progression-To determine the functional significance of Nup96 downregulation in mitosis, [investigators] investigated whether Nup96, if present at high levels, has effects on mitotic timing. (harvard.edu)
  • They] generated stably transfected HeLa cell lines expressing high levels of FLAG-tagged-Nup96 (data not shown) and coexpressed histone H2B-YFP to follow live cells as they transitioned through mitosis. (harvard.edu)
  • In humans Bub1 accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. (wikipedia.org)
  • Disruption of the ompA gene in the tdcA mutant strain abolished the hyperadherent phenotype, and anti-OmpA serum inhibited adhesion of wild-type and tdcA mutant strains to HeLa cells. (asm.org)
  • Enhanced adhesion mediated by OmpA was also observed with Caco-2 cells, and anti-OmpA serum blocked adherence to HeLa cells of other EHEC O157:H7 strains. (asm.org)
  • We recently characterized a fimbrial operon in EHEC O157:H7 that mediates adherence when introduced into a nonfimbriated E. coli K-12 strain, but EHEC strains mutated in this operon were only modestly reduced in adherence to HeLa cells ( 39 ). (asm.org)
  • The ImageXpress® Pico system does more than imaging-it offers unparalleled analysis capabilities that simplifies image analysis for cell-based assays. (moleculardevices.com)
  • By complement fixation assays, the antisera reacted with HeLa cell chromatin but only marginally with human placenta chromatin. (aacrjournals.org)
  • These cells are used for VIRUS CULTIVATION and antitumor drug screening assays. (umassmed.edu)
  • A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parson's lab at King's College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinson's. (biologists.org)
  • HeLa cells dividing under electron microscopy. (howstuffworks.com)
  • These strategies were compared through the semantic segmentation of the nucleus, nuclear envelope, cell and background of three hundred slices of a HeLa cell observed with electron microscopy. (eurekalert.org)
  • In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. (sigmaaldrich.com)
  • But her cells lived on and have helped scientists develop the polio vaccine, in vitro fertilization and an array of treatments for cancer and other diseases. (npr.org)
  • Known as HeLa cells, their stunning potency gave scientists a building block for countless breakthroughs, beginning with the cure for polio. (hastac.org)
  • Readers learn not just about HeLa cells and John Moore but also about the development of polio vaccine, HIPAA, the Jewish Chronic Disease Hospital cancer studies, and the history of Johns Hopkins hospital. (thefreedictionary.com)
  • Jonas Salk had developed a polio vaccine in the early 1950's but was struggling to find a way to test it in field trials as traditionally used rhesus monkey cells were too expensive for such a large-scale study. (technologynetworks.com)
  • In 1952, HeLa cells were found to be both susceptible to, but not killed by polio, making them an ideal source of host cells. (technologynetworks.com)
  • During the mass production and distribution of HeLa cells for polio vaccine testing at Tuskegee University, lead researchers Brown and Henderson pioneered new cell culture protocols , such as the use of rubber-lined screw-capped bottles and tubes and strict QC measures. (technologynetworks.com)
  • Named after a cancer patient, the cells were taken from Lacks' tissue samples and grown by a researcher named Dr. George Gey in 1951. (howstuffworks.com)
  • The cells were propagated by Gey shortly before Lacks died of her cancer in 1951. (wikipedia.org)
  • The bacteria contained in the isolated mature vesicles had a unique envelope structure and were highly adherent to HeLa cells, characteristics that correlated with a bright red appearance after the Giménez stain (Giménez positive). (nih.gov)
  • Increased expression of unknown genes in tumorigenic compared to non-tumorigenic hybrid cells: eg. (aacrjournals.org)
  • From 12-96 hours post irradiation a significant fraction of the genes with altered expression were found to be involved in cell cycle progression and its regulation. (diva-portal.org)
  • 8, 9] These findings suggest that cytoplasmic genes may have a wider role in the cell than hitherto suspected and may direct a considerable fraction of mRNA and protein synthesis. (caltech.edu)
  • The authors established the protein copy numbers of 12,209 protein-coding genes in HeLa cells, which is comparable to the coverage of the achieved by next-generation RNA-Seq technology. (ku.dk)
  • It is challenging to determine why, from the multiple regions in the EHEC O157:H7 chromosome with a putative role in adherence, only a limited number of genes within the LEE and other loci have been suggested to be involved in the adherence of these organisms to host cells. (asm.org)
  • Treatment with the plant extract showed distribution of cells between late apoptotic and necrotic phases and caused cell cycle arrest at the G0/G1 phase. (omicsonline.org)
  • Results demonstrated that R. arboreum could be a potential anti-cancer agent by its mechanism of inducing apoptotic cell death and cell cycle arrest in HeLa cells. (omicsonline.org)
  • In the present study, the effects of exogenous H2O2 on cell growth and death in HeLa cervical cancer cells were investigated, and the anti-apoptotic effects of various caspase (pan-caspase, caspase-3, -8 or -9) inhibitors on H2O2-treated HeLa cells were also evaluated with regard to reactive oxygen species (ROS) and glutathione (GSH) levels. (spandidos-publications.com)
  • This leads to the publication of scientific articles in which the authors report what they believe to be their findings on skin cancer in humans, whereas their assumptions were based on studying mouse cells. (eurekalert.org)
  • Western blot analysis was performed on extracts derived from the same cells to verify the efficacy of the hIFN-α1 treatment. (cellsignal.com)
  • The effect of the different extracts on HeLa cells was studied over a 3-h period. (thefreedictionary.com)
  • This wasn't limited to HeLa alone, but was also observed in other immortal cell lines. (eurekalert.org)
  • There it was discovered that the cells were "immortal", that they keep dividing infinitely. (tanzforumberlin.de)
  • These findings were presented at the Second Decennial Review Conference on Cell Tissue and Organ Culture 4 and have since been extended to include other American Type Culture Collection lines. (nature.com)
  • A sample of her cancerous tissue, taken without her knowledge or consent, as was the custom then, turned out to provide one of the holy grails of mid-century biology: human cells that could survive--even thrive--in the lab. (hastac.org)
  • 2 When tissue cells are resistant to apoptosis, it may promote cancer growth and impede the effectiveness of cancer therapies. (spiedigitallibrary.org)
  • The sequential cytological changes which develop in tissue culture cells infected with adenovirus types 5 and 7 are described and compared with those produced by adenovirus types 1, 2, 3, and 4. (rupress.org)
  • Exogenous H 2 O 2 is often applied as the representative ROS in modeling oxidative stress in the cell and tissue. (spandidos-publications.com)
  • Communication between tissue donors and doctors was virtually nonexistent (i.e. cells were taken without patient consent, nor were they told what the cells would be used for). (wikipedia.org)
  • The court ruled that a person's discarded tissue and cells are not his or her property and can be commercialized. (wikipedia.org)
  • HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. (abcam.com)
  • Conclusion: Differential gene expression of a so far unknown gene on chromosome 11q13 may play a central role in determination for in vivo growth potential of non-tumorigenic and tumorigenic HeLa x fibroblast hybrid cells. (aacrjournals.org)
  • Recently, two Nobel prizes have been awarded for discoveries where HeLa cells played a central role, namely the link between human papilloma virus and cervical cancer (2008, Harald zur Hausen) and the role of telomerase in preventing chromosome degradation (2011, Elizabeth Blackburn, Carol Greider, and Jack Szostak). (g3journal.org)
  • The protein kinase Bub1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase. (wikipedia.org)
  • Analysis of human nuclear phosphorylation sites by multidimensional LC coupled to MS/MS/MS. ( A ) Eight milligrams of nuclear extract from asynchronous HeLa cells were separated by SDS/PAGE. (pnas.org)
  • The cell will finish dividing its nuclear material and then cytoplasm in order to physically separate into two daughter cells. (wellcomecollection.org)
  • HeLa cells counted using StainFree™ Cell Detection Technology (blue circles) and red nuclear stain (red circles). (moleculardevices.com)
  • Coommassie stained SDS-PAGE of 25 ?g of Doxorubicin stimulated Human Derived Hela Cell Nuclear Extract (Ready-to-Use) separated in a 4-20% gradient gel under non-reducing conditions. (antikoerper-online.de)
  • Fractionation of HeLa cells into three cytoplasmic and several nuclear fractions showed that almost all the antigens recognized by antisera to dehistonized chromatin were nuclear. (aacrjournals.org)
  • Procedures for the isolation of HeLa S3 nuclear matrices were re-examined with special emphasis on the use of various nucleases and detergents as well as on the ionic strength of the final salt extraction. (biologists.org)
  • As a result, nanoviscosity affects all diffusion-based interactions in living cell, including biosynthesis, signalling pathways or even active transport 5 . (nature.com)
  • It is also found that functionalized disc-shaped zeolite L particles enter the cancer cells via different, partly not yet characterized, pathways. (wiley.com)
  • Our analysis of the HeLa gene expression profile revealed that several pathways, including cell cycle and DNA repair, exhibit significantly different expression patterns from those in normal human tissues. (g3journal.org)
  • The mechanism of apoptosis generally involves two signaling pathways, the mitochondrial pathway and the cell death receptor pathway ( 14 - 16 ). (spandidos-publications.com)
  • To address this limitation, we mined the literature to obtain a compendium of in vitro hyperthermia experiments investigating the heat-shock response in HeLa cells. (nih.gov)
  • Triterpenoids from Schisandra henryi with cytotoxic effect on leukemia and Hela cells in vitro," Archives ofPharmacal Research, vol. (thefreedictionary.com)
  • Aim of study: Analysis of in vitro and in vivo growth properties, differential chromosomal aberrations and differential gene expression patterns of non-tumorigenic hybrid cells compared to tumorigenic segregants and tumorigenic parental HeLa cells by cDNA microarray analysis. (aacrjournals.org)
  • Results: Confirmation of similar expression levels of HPV18 viral oncogenes and in vitro and in vivo growth properties in non-tumorigenic and tumorigenic cells. (aacrjournals.org)
  • Interactions are studied in terms of their fates under diverse in vitro cell culture conditions. (wiley.com)
  • These in vitro results provide additional insight about low-aspect ratio anisotropic nanoparticle interactions with cancer cells and demonstrate the possibility to manipulate the interactions of nanoparticles and cells by surface coating for the use of nanoparticles in medical applications. (wiley.com)
  • and Efa-1, an adhesin from non-O157 Shiga toxin-producing E. coli serotypes necessary for in vitro adhesion to CHO cells and for colonization of the bovine intestine ( 17 , 33 ). (asm.org)
  • 3. Resuspend the cell pellet with 1 mL pre-warmed complete medium DMEM+10% FBS and dispense into a 25 cm 2 culture flask containing 10 mL pre-warmed complete complete medium DMEM+10% FBS. (abcam.com)
  • Hello, From a T75 flask of subconfluent HeLa cells, I extracted about: 3ug/ul. (protocol-online.org)
  • Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10 4 cells/cm 2 . (abcam.com)
  • I used regular HeLa cells (75cm2 flask) and scrape them in 500ul LB (+Protease inhibitors) after letting them on ice for 20 min. (protocol-online.org)
  • It consists of several steps of processing in which features are highlighted and used to ultimately identify the nucleus of the cell and the membrane surrounding it. (eurekalert.org)
  • Therefore, we used H2 ER [alpha]-GFP transfected into HeLa cells to test D3 binding by visualizing that D3 increases the translocation of ER [alpha] to the nucleus. (thefreedictionary.com)
  • The most important organelle in the cell is the nucleus. (artscolumbia.org)
  • The nucleus holds all of the blueprint information for the cell. (artscolumbia.org)
  • The DNA of a cell is found in the nucleus along with RNA. (artscolumbia.org)
  • In between the membrane of the nucleus and the cell membrane is cytoplasm. (artscolumbia.org)
  • That the host cell nucleus is the principal site of adenovirus synthesis has been confirmed by fluorescent antibody studies. (rupress.org)
  • Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. (abcam.com)
  • The cells were grown in DMEM supplemented with 10% FBS (Fetal Bovine Serum). (antikoerper-online.de)
  • The cells were incubated for 48 h in Dulbecco's minimal essential medium supplemented with 10% heat-inactivated fetal bovine serum and antibiotics. (asm.org)
  • Cell-biology] Markers, detection and significance of disseminated breast cancer cells. (bio.net)
  • HeLa cells as a model to study the invasiveness and biology of Legionella pneumophila. (nih.gov)
  • To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. (thermofisher.com)
  • Scientists' expanding knowledge and understanding of stem cells is transforming the way we research human biology and medicine. (moleculardevices.com)
  • This study underscores the importance of accounting for the strikingly aberrant characteristics of HeLa cells when designing and interpreting experiments, and has implications for the use of HeLa as a model of human biology. (g3journal.org)
  • We are now accepting submissions for our upcoming special issue on 'Reconstituting cell biology', guest edited by Manuel Théry. (biologists.org)
  • RNA·DNA hybridization experiments utilizing separated strands of HeLa mitochondrial DNA and mit-RNA from HeLa cells exposed to short pulses of [5-3H]uridine have shown that the labeled RNA hybridizes with both the light (L) and the heavy (H) strand, though to a different relative extent depending upon the labeling time. (caltech.edu)
  • These observations and the previous evidence of complete transcription of the H strand strongly suggest that mit-DNA is transcribed in HeLa cells symmetrically over a considerable portion of its length, with the transcript of the L strand being rapidly degraded or otherwise removed from the mitochondrial fraction. (caltech.edu)
  • Find tissues and cell lines supported by DNA array analysis to express PAPSS2 . (avivasysbio.com)
  • The technology has been streamlined for one day mass spectrometric measurements of human proteomes from cells and tissues. (ku.dk)
  • Description For cells, cell lines and tissues in culture till half confluency. (gentaur.com)
  • Here, we describe an optimized protocol for the analysis of relevant parameters of autophagic fl ux using HeLa cells stably expressing EGFP-LC3. (csic.es)
  • Materials and Methods: Differential gene expression patterns were analysed in non-tumorigenic and tumorigenic hybrid cells, parental HeLa cells and normal human fibroblasts by microarray analysis using Hu133Plus2.0 chips by Affymetrix. (aacrjournals.org)
  • Furthermore, biological responses linked to mitotic catastrophe including cell cycle arrests, anaphase bridge formation and centrosome amplification were analyzed and correlation to gene expression changes evaluated. (diva-portal.org)
  • This study elucidates specific characteristics in the altered gene expression pattern induced by irradiation, which can be linked to the sequential steps observed in HeLa Hep2 cells during mitotic catastrophes. (diva-portal.org)
  • Nucleoporin levels regulate cell cycle progression and phase-specific gene expression. (harvard.edu)
  • Cells were treated with 0.5 µg/ml Doxorubicin for 2 h. (antikoerper-online.de)
  • Autophagy was then promoted in HeLa cells pharmacologically by Doxorubicin and genetically by siRNA IL-10. (medworm.com)
  • This promotes the charge separation and transfer processes and can produce more reactive oxygen species (ROS) that are responsible for the killing of HeLa cells. (springer.com)
  • The latter interaction is essential for kinetochore localization of Bub1 and its function in cell cycle arrest induced by spindle assembly checkpoint (SAC) activation. (wikipedia.org)