AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
HeLa CellsMolecular Sequence DataBase SequenceCell NucleusTransfectionPoliovirusTranscription, GeneticCell LineAmino Acid SequenceDNA-Binding ProteinsRNA, MessengerPromoter Regions, GeneticNuclear ProteinsAdenoviruses, HumanMitosisDNAKineticsProtein BindingPlasmidsTranscription FactorsCloning, MolecularMolecular WeightRibonucleoproteinsRecombinant Fusion ProteinsApoptosisProtein BiosynthesisCell CycleCell ExtractsCytoplasmGene Expression RegulationViral ProteinsMutationDNA ReplicationPhosphorylationElectrophoresis, Polyacrylamide GelRNA, Small InterferingRecombinant ProteinsUridineMicroscopy, FluorescenceRNACell SurvivalCell MembraneCell NucleolusRNA InterferenceBacterial AdhesionFluorescent Antibody TechniqueRhinovirusCarrier ProteinsRNA SplicingVirus ReplicationRNA, Heterogeneous NuclearInterphaseRNA, ViralGreen Fluorescent ProteinsAdenovirus Early ProteinsCentrifugation, Density GradientRNA PrecursorsCell DivisionVaccinia virusProtein TransportBlotting, WesternDactinomycinLuminescent ProteinsRibonucleoproteins, Small NuclearEnterovirus B, HumanHeterogeneous-Nuclear RibonucleoproteinsRNA-Binding ProteinsDNA, ComplementaryCell-Free SystemCytotoxinsHot TemperatureTritiumGenesCycloheximideRNA Cap-Binding ProteinsChloramphenicol O-AcetyltransferaseShigella flexneriPrecipitin TestsTime FactorsMembrane ProteinsMitochondriaBiological TransportRNA, Small NuclearGene ExpressionDNA, ViralTumor Cells, CulturedTissue Culture TechniquesRegulatory Sequences, Nucleic Acid
HeLa Cells
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Transfection
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Poliovirus
A species of ENTEROVIRUS which is the causal agent of POLIOMYELITIS in humans. Three serotypes (strains) exist. Transmission is by the fecal-oral route, pharyngeal secretions, or mechanical vector (flies). Vaccines with both inactivated and live attenuated virus have proven effective in immunizing against the infection.
Transcription, Genetic
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
DNA-Binding Proteins
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Promoter Regions, Genetic
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Nuclear Proteins
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Adenoviruses, Human
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
Mitosis
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Kinetics
The rate dynamics in chemical or physical systems.
Protein Binding
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Plasmids
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Transcription Factors
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Molecular Weight
The sum of the weight of all the atoms in a molecule.
Ribonucleoproteins
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
Recombinant Fusion Proteins
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Protein Biosynthesis
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Cell Cycle
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Cell Extracts
Preparations of cell constituents or subcellular materials, isolates, or substances.
Cytoplasm
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Gene Expression Regulation
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Viral Proteins
Proteins found in any species of virus.
Mutation
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
DNA Replication
The process by which a DNA molecule is duplicated.
Phosphorylation
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Electrophoresis, Polyacrylamide Gel
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Recombinant Proteins
Proteins prepared by recombinant DNA technology.
Uridine
Microscopy, Fluorescence
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Cell Survival
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Cell Membrane
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Cell Nucleolus
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
RNA Interference
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Bacterial Adhesion
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Rhinovirus
A genus of PICORNAVIRIDAE inhabiting primarily the respiratory tract of mammalian hosts. It includes over 100 human serotypes associated with the COMMON COLD.
Carrier Proteins
Transport proteins that carry specific substances in the blood or across cell membranes.
RNA Splicing
The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.
Virus Replication
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
RNA, Heterogeneous Nuclear
Nuclear nonribosomal RNA larger than about 1000 nucleotides, the mass of which is rapidly synthesized and degraded within the cell nucleus. Some heterogeneous nuclear RNA may be a precursor to mRNA. However, the great bulk of total hnRNA hybridizes with nuclear DNA rather than with mRNA.
Interphase
The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).
RNA, Viral
Ribonucleic acid that makes up the genetic material of viruses.
Green Fluorescent Proteins
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Adenovirus Early Proteins
Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.
Centrifugation, Density Gradient
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
RNA Precursors
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
Cell Division
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Vaccinia virus
The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.
Protein Transport
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Blotting, Western
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Dactinomycin
A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)
Luminescent Proteins
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Ribonucleoproteins, Small Nuclear
Highly conserved nuclear RNA-protein complexes that function in RNA processing in the nucleus, including pre-mRNA splicing and pre-mRNA 3'-end processing in the nucleoplasm, and pre-rRNA processing in the nucleolus (see RIBONUCLEOPROTEINS, SMALL NUCLEOLAR).
Enterovirus B, Human
A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.
Heterogeneous-Nuclear Ribonucleoproteins
A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.
RNA-Binding Proteins
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
DNA, Complementary
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Cell-Free System
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Cytotoxins
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
Hot Temperature
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Tritium
Genes
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Cycloheximide
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
RNA Cap-Binding Proteins
Proteins that specifically bind to RNA CAPS and form nuclear cap binding protein complexes. In addition to stabilizing the 5' end of mRNAs, they serve a diverse array of functions such as enhancing mRNA transport out of the CELL NUCLEUS and regulating MRNA TRANSLATION in the CYTOPLASM.
Chloramphenicol O-Acetyltransferase
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Shigella flexneri
A bacterium which is one of the etiologic agents of bacillary dysentery (DYSENTERY, BACILLARY) and sometimes of infantile gastroenteritis.
Precipitin Tests
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Time Factors
Elements of limited time intervals, contributing to particular results or situations.
Membrane Proteins
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Mitochondria
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Biological Transport
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
RNA, Small Nuclear
Short chains of RNA (100-300 nucleotides long) that are abundant in the nucleus and usually complexed with proteins in snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR). Many function in the processing of messenger RNA precursors. Others, the snoRNAs (RNA, SMALL NUCLEOLAR), are involved with the processing of ribosomal RNA precursors.
Gene Expression
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
DNA, Viral
Deoxyribonucleic acid that makes up the genetic material of viruses.
Tumor Cells, Cultured
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Tissue Culture Techniques
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Regulatory Sequences, Nucleic Acid
Nucleic acid sequences involved in regulating the expression of genes.

The incorporation of 5-iodo-2'-deoxyuridine into the DNA of HeLa cells and the induction of alkaline phosphatase activity. (1/37241)

Inhibition of DNA synthesis during the period of exposure of HeLa cells to 5-iodo-2'-deoxyuridine (IUdR) inhibited the induction of alkaline phosphatase activity. This finding, taken together with previous findings that IUdR did not induce alkaline phosphatase activity in the presence of 2-fold molar excess thymidinemonstrated that IUdR incorporation into DNA is correlated with the increase in alkaline phosphatase activity. With the exception of an interim period described in the text, induction of alkaline phosphatase activity was linearly related to medium concentrations of IUdR of up to at least 3 muM. However, the extent of IUdR substitution in DNA did not appear to be related to the degree of enzyme induction. Alkaline phosphatase activity continued to increase at medium concentrations of IUdR from 1 to 3 muM, while little further substitution of DNA occurred.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (2/37241)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (3/37241)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite. (4/37241)

The reaction of HeLa-cell 5.8S rRNA with NaHSO3 under conditions in which exposed cytidine residues are deaminated to uridine was studied. It was possible to estimate the reactivities of most of the 46 cytidine residues in the nucleotide sequence by comparing 'fingerprints' of the bisulphite-treated RNA with those of untreated RNA. The findings were consistent with the main features of the secondary-structure model for mammalian 5.85S rRNA proposed by Nazar, Sitz, & Busch [J. Biol. Chem (1975) 250, 8591--8597]. Five out of six regions that are depicted in the model as single-stranded loops contain cytidine residues that are reactive towards bisulphite at 25 degrees C (the other loop contains no cytidine). The cytidine residue nearest to the 3'-terminus is also reactive. Several cytidines residues that are internally located within proposed double-helical regions show little or no reactivity towards bisulphite, but the cytidine residues of several C.G pairs at the ends of helical regions show some reactivity, and one of the proposed loops appears to contain six nucleotides, rather than the minimum of four suggested by the primary structure. Two cytidine residues that are thought to be 'looped out' by small helix imperfections also show some reactivity.  (+info)

Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. (5/37241)

Studies of the actin-based motility of pathogens have provided important insights into the events occurring at the leading edge of motile cells [1] [2] [3]. To date, several actin-cytoskeleton-associated proteins have been implicated in the motility of Listeria or Shigella: vasodilator-stimulated phosphoprotein (VASP), vinculin and the actin-related protein complex of Arp2 and Arp3 [4] [5] [6] [7]. To further investigate the underlying mechanism of actin-tail assembly, we examined the localization of components of the actin cytoskeleton including Arp3, VASP, vinculin and zyxin during vaccinia, Listeria and Shigella infections. The most striking difference between the systems was that a phosphotyrosine signal was observed only at the site of vaccinia actin-tail assembly. Micro-injection experiments demonstrated that a phosphotyrosine protein plays an important role in vaccinia actin-tail formation. In addition, we observed a phosphotyrosine signal on clathrin-coated vesicles that have associated actin-tail-like structures and on endogenous vesicles in Xenopus egg extracts which are able to nucleate actin tails [8] [9]. Our observations indicate that a host phosphotyrosine protein is required for the nucleation of actin filaments by vaccinia and suggest that this phosphoprotein might be associated with cellular membranes that can nucleate actin.  (+info)

Bcl-2 regulates amplification of caspase activation by cytochrome c. (6/37241)

Caspases, a family of specific proteases, have central roles in apoptosis [1]. Caspase activation in response to diverse apoptotic stimuli involves the relocalisation of cytochrome c from mitochondria to the cytoplasm where it stimulates the proteolytic processing of caspase precursors. Cytochrome c release is controlled by members of the Bcl-2 family of apoptosis regulators [2] [3]. The anti-apoptotic members Bcl-2 and Bcl-xL may also control caspase activation independently of cytochrome c relocalisation or may inhibit a positive feedback mechanism [4] [5] [6] [7]. Here, we investigate the role of Bcl-2 family proteins in the regulation of caspase activation using a model cell-free system. We found that Bcl-2 and Bcl-xL set a threshold in the amount of cytochrome c required to activate caspases, even in soluble extracts lacking mitochondria. Addition of dATP (which stimulates the procaspase-processing factor Apaf-1 [8] [9]) overcame inhibition of caspase activation by Bcl-2, but did not prevent the control of cytochrome c release from mitochondria by Bcl-2. Cytochrome c release was accelerated by active caspase-3 and this positive feedback was negatively regulated by Bcl-2. These results provide evidence for a mechanism to amplify caspase activation that is suppressed at several distinct steps by Bcl-2, even after cytochrome c is released from mitochondria.  (+info)

The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. (7/37241)

The cellular protein p32 was isolated originally as a protein tightly associated with the essential splicing factor ASF/SF2 during its purification from HeLa cells. ASF/SF2 is a member of the SR family of splicing factors, which stimulate constitutive splicing and regulate alternative RNA splicing in a positive or negative fashion, depending on where on the pre-mRNA they bind. Here we present evidence that p32 interacts with ASF/SF2 and SRp30c, another member of the SR protein family. We further show that p32 inhibits ASF/SF2 function as both a splicing enhancer and splicing repressor protein by preventing stable ASF/SF2 interaction with RNA, but p32 does not block SRp30c function. ASF/SF2 is highly phosphorylated in vivo, a modification required for stable RNA binding and protein-protein interaction during spliceosome formation, and this phosphorylation, either through HeLa nuclear extracts or through specific SR protein kinases, is inhibited by p32. Our results suggest that p32 functions as an ASF/SF2 inhibitory factor, regulating ASF/SF2 RNA binding and phosphorylation. These findings place p32 into a new group of proteins that control RNA splicing by sequestering an essential RNA splicing factor into an inhibitory complex.  (+info)

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (8/37241)

Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. A stable complex containing both Zta and CBP could be isolated from lytically stimulated, but not latently infected RAJI nuclear extracts. Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1ADelta2-36), which fails to bind CBP. Zta bound directly to two related cysteine- and histidine-rich domains of CBP, referred to as C/H1 and C/H3. These domains both interacted specifically with the transcriptional activation domain of Zta in an electrophoretic mobility shift assay. Interestingly, we found that the C/H3 domain was a potent dominant negative inhibitor of Zta transcriptional activation function. In contrast, an amino-terminal fragment containing the C/H1 domain was sufficient for coactivation of Zta transcription and viral reactivation function. Thus, CBP can stimulate the transcription of latent EBV in a histone acetyltransferase-independent manner mediated by the CBP amino-terminal C/H1-containing domain. We propose that CBP may regulate aspects of EBV latency and reactivation by integrating cellular signals mediated by competitive interactions between C/H1, C/H3, and the Zta activation domain.  (+info)

Hypoxia stimulates invasion and migration of human cervical cancer cell lines HeLa/SiHa through the Rab11 trafficking of...
Hypoxia plays a key role in tumour cell survival, invasion, and metastasis. An increasing number of studies have attempted to characterize the tumour response to hypoxia and to identify predictive markers of disease. Here we show that hypoxia increases tumour cell invasion and migration by the modulation of Rab11, an important molecule for vesicular trafficking. In our study, we found that Rab11, together with the activation of Rac1, could stimulate invasion and migration of cervical cancer cell lines HeLa/SiHa in hypoxia. Activation of Rac1 activity by hypoxia seems to be central to carcinoma invasion. We also found that these effects could be related to the integrin αvβ3. In addition, we studied the molecular pathway for this process. Our results showed that in cervical cancer cell... lines HeLa/SiHa, Rac1 activation in hypoxia could stimulate invasion and migration, and this process was mediated by integrin αvβ3-mediated FAK and PI3K phosphorylation. Furthermore, hypoxia induced a ...
http://d.scholar.cnki.net/detail/SSJDTEMP_U/SSJD11B5D09FC5D6C28129E1A6F9CE8167D1
AID 87350 - Compound was tested for antiviral activity in HeLa Cell cultures against herpes simplex virus type 1 (HSV-1) -...AID 87350 - Compound was tested for antiviral activity in HeLa Cell cultures against herpes simplex virus type 1 (HSV-1) -...
BioAssay record AID 87350 submitted by ChEMBL: Compound was tested for antiviral activity in HeLa Cell cultures against herpes simplex virus type 1 (HSV-1).
https://pubchem.ncbi.nlm.nih.gov/bioassay/87350
HeLa cells in culture, light microscopy - Stock Video Clip K005/6160 - Science Photo LibraryHeLa cells in culture, light microscopy - Stock Video Clip K005/6160 - Science Photo Library
HeLa cells in culture. Light microscopy footage of the movement, division and death of HeLa cervical cancer cells. HeLa cells are a continuously cultured cell line of human cancer cells. They are immortal, in that they can divide an unlimited number of times. However, they are also poorly differentiated and lack some of the characteristics of normal cells. HeLa cells are widely used in biological and medical research. They all originate from a sample taken in 1951 from cervical cancer patient Henrietta Lacks, after whom they are named. - Stock Video Clip K005/6160
http://www.sciencephoto.com/media/728732/view
HeLa cell, SEM - Stock Image C011/1346 - Science Photo LibraryHeLa cell, SEM - Stock Image C011/1346 - Science Photo Library
HeLa cell. Coloured scanning electron micrograph (SEM) of a cultured Hela cell (centre) on MDCK kidney cells (background). HeLa cells are a continuously cultured cell line of human cancer cells, which are immortal and so thrive in the laboratory. Numerous microvilli (string-like) can be seen, which cancer cells use to enable them to move around and spread (metastasise) from one area to another. MDCK cells are a strain of cultured, normal kidney epithelial cells. - Stock Image C011/1346
http://www.sciencephoto.com/media/432225/view
AID 506767 - Binding affinity to SAP145 in SAP145-targeting siRNA-treated human HeLa cells at 1 uM by fluorescent microscopy -...AID 506767 - Binding affinity to SAP145 in SAP145-targeting siRNA-treated human HeLa cells at 1 uM by fluorescent microscopy -...
BioAssay record AID 506767 submitted by ChEMBL: Binding affinity to SAP145 in SAP145-targeting siRNA-treated human HeLa cells at 1 uM by fluorescent microscopy.
https://pubchem.ncbi.nlm.nih.gov/bioassay/506767
SHSY5Y and HeLa cells were transiently transfected with scRNA (SHSY5Y/scRNA and HeLa/scRNA) or -Syn-siRNA (SHSY5Y/-Syn-siRNA...SHSY5Y and HeLa cells were transiently transfected with scRNA (SHSY5Y/scRNA and HeLa/scRNA) or -Syn-siRNA (SHSY5Y/-Syn-siRNA...
SHSY5Y and HeLa cells were transiently transfected with scRNA (SHSY5Y/scRNA and HeLa/scRNA) or -Syn-siRNA (SHSY5Y/-Syn-siRNA and HeLa/-Syn-siRNA) for 48 h. in malignancy cells. RESULTS TTP overexpression promotes an elongation of mitochondria Previously, we reported that overexpression of TTP suppresses cellular proliferation [46, 47, 50] and induces a change in cell morphology from a mesenchymal shape to an epithelial shape [51]. Here we assessed whether TTP overexpression modifies mitochondrial morphology. To test this, SHSY5Y neuroblastoma cells and HeLa cervical carcinoma cells were transiently transfected with pcDNA6/V5-TTP (SHSY5Y/TTP and HeLa/TTP) or a control pcDNA6/V5 (SHSY5Y/pcDNA and HeLa/pcDNA) vector. After confirming the overexpression of TTP by RT-PCR and western blot analysis (Number ?(Figure1A),1A), mitochondria in the cells were stained with Mitotracker. Confocal microscopic imaging of mitochondria showed that TTP overexpression advertised the elongation of the mitochondrial ...
http://pacfs.org/%EF%BB%BFshsy5y-and-hela-cells-were-transiently-transfected-with-scrna-shsy5y-scrna-and-hela-scrna-or-syn-sirna-shsy5y-syn-sirna-and-hela-syn-sirna-for-48-h/
Silencing oncogene expression in cervical cancer stem-like cells inhibits their cell growth and self-renewal ability | Cancer...Silencing oncogene expression in cervical cancer stem-like cells inhibits their cell growth and self-renewal ability | Cancer...
Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for tumor initiation and maintenance. They are also considered as an attractive target for advanced cancer therapy. Using a sphere culture method that favors the growth of self-renewal cells, we have isolated sphere-forming cells (SFCs) from cervical cancer cell lines HeLa and SiHa. HeLa-SFCs were resistant to multiple chemotherapeutic drugs and were more tumorigenic, as evidenced by the growth of tumors following injection of immunodeficient mice with 1 × 104 cells, compared with 1 × 106 parental HeLa cells required to grow tumors of similar size in the same time frame. These cells showed an expression pattern of CD44high/CD24low that resembles the CSC surface biomarker of breast cancer. We further demonstrated that HeLa-SFCs expressed a higher level (6.9-fold) of the human papillomavirus oncogene E6, compared with that of parental HeLa cells. Gene silencing of E6 with a lentiviral-short-hairpin RNA (shRNA)
https://www.nature.com/articles/cgt201158?error=cookies_not_supported&code=13503dce-0161-4109-b063-2f3db3d4d0a6
OSA | Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillatorOSA | Intracellular disruption of mitochondria in a living HeLa cell with a 76-MHz femtosecond laser oscillator
Fig. 6. Mitotic events of cell division after disruption of mitochondria in the histone EGFP-H1 expressed cell. The disruption of a single mitochondrion by femtosecond laser irradiation had no influence on cell division or cell activity. The cell nuclei and mitotic chromosomes in HeLa cells were visualized using histone EGFP-H1. Mitochondria were stained with MitoTracker Red. Confocal fluorescence image and transmission image (a) before and (b) after femtosecond laser irradiation with 0.39 nJ/pulse (exposure time: 32 ms). The yellow arrow indicates the irradiation point. (c)-(f) Time-lapse confocal images and transmission images. The mitotic events of cell division in the irradiated cells proceeded normally. Scale bar: 20 μm ...
http://proxy.osapublishing.org/oe/abstract.cfm?uri=oe-13-24-9869
So what else is new?So what else is new?
Im pretty sure the fact that HeLa cells are abnormal has been known more or less since they were first isolated. That said, how they are abnormal has not. And this is very relevant - if researchers are using HeLa cells to study something, and HeLa cells are abnormal along that axis, then their data are suspect. So knowing how they are normal, and abnormal, is important information. This is why the news that the transformation of healthy non-cancerous cervical cells into HeLa cells happened due to human papilloma virus infection - that is, Henrietta Lacks (the source of the cells) had HPV, which gave her the cancer that ultimately killed her. This is noteworthy because it establishes the mutation(s) and phenotype(s) that result when cervical cells are infected with HPV (with ramifications both for work with HeLa and other cervical cancer lines AND for care for patients).. Remember also that HeLa was the very first immortalized cell line. Studies on HeLa led to the immortalization of other cells, ...
https://freethoughtblogs.com/pharyngula/2013/03/20/so-what-else-is-new/comment-page-1/
YUAN YUANYUAN YUAN
Hypoxia plays a key role in tumour cell survival, invasion, and metastasis. An increasing number of studies have attemptedto characterize the tumour response to hypoxia and to identify predictive markers of disease. Here we show that hypoxiaincreases tumour cell invasion and migration by the modulation of Rab11, an important molecule for vesicular trafficking.In our study, we found that Rab11, together with the activation of Rac1, could stimulate invasion and migration of cervicalcancer cell lines HeLa/SiHa in hypoxia. Activation of Rac1 activity by hypoxia seems to be central to carcinoma invasion.We also found that these effects could be related to the integrin αvβ3. In addition, we studied the molecular pathway for thisprocess. Our results showed that in cervical cancer cell lines HeLa/SiHa, Rac1 activation in hypoxia could stimulateinvasion and migration, and this process was mediated by integrin αvβ3-mediated FAK and PI3K phosphorylation.Furthermore, hypoxia induced a dramatic increase ...
https://www.ias.ac.in/listing/bibliography/jbsc/YUAN_YUAN
Dividing HeLa cells,… | Wellcome CollectionDividing HeLa cells,… | Wellcome Collection
Light micrograph of dividing HeLa cells. Chromosomes in the cell nucleus (purple), microtubules in the cell cytoskeleton (tubulin; green), and actin (red) are visible here. In the cell in the centre of the image, condensed chromosomes (purple) have aligned and attached to the spindle (green). During anaphase (one of the stages of nuclear division in mitosis), the spindle pulls the condensed chromosomes to opposite poles of the cell. The cell will finish dividing its nuclear material and then cytoplasm in order to physically separate into two daughter cells. HeLa cells are an immortal human epithelial cell line derived from a cancerous tumour of the cervix (adenocarcinoma). It was established in 1951 from a biopsy taken from Henrietta Lacks and was the first human cell line to survive and grow in the laboratory. Henriettas cells were originally used in this way without permission from her or her family which raises issues about ethics and privacy. HeLa cells have been used extensively around the world
https://wellcomecollection.org/works/vjq5c26r?query=chromosomes+histones
HeLa cell legal definition of HeLa cellHeLa cell legal definition of HeLa cell
Definition of HeLa cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is HeLa cell? Meaning of HeLa cell as a legal term. What does HeLa cell mean in law?
https://legal-dictionary.thefreedictionary.com/HeLa+cell
Comprehensive circular RNA expression profile in radiation-treated HeLa cells and analysis of radioresistance-related circRNAs ...Comprehensive circular RNA expression profile in radiation-treated HeLa cells and analysis of radioresistance-related circRNAs ...
Background Cervical cancer is one of the most common cancers in women worldwide. Malignant tumors develop resistance mechanisms and are less sensitive to or do not respond to irradiation. With the development of high-throughput sequencing technologies, circular RNA (circRNA) has been identified in an increasing number of diseases, especially cancers. It has been reported that circRNA can compete with microRNAs (miRNAs) to change the stability or translation of target RNAs, thus regulating gene expression at the transcriptional level. However, the role of circRNAs in cervical cancer and the radioresistance mechanisms of HeLa cells are unknown. The objective of this study is to investigate the role of circRNAs in radioresistance in HeLa cells. Methods High-throughput sequencing and bioinformatics analysis of irradiated and sham-irradiated HeLa cells. The reliability of high-throughput RNA sequencing was validated using quantitative real-time polymerase chain reaction. The most significant circRNA
https://peerj.com/articles/5011/
Cytotoxic Activities of <i>Funalia trogii</i> (Berk.) Bond. Et. Singer ATCC 200800 Bioactive Extract on HeLa Cells and...Cytotoxic Activities of <i>Funalia trogii</i> (Berk.) Bond. Et. Singer ATCC 200800 Bioactive Extract on HeLa Cells and...
This study demonstrated the cytotoxic and cell growth inhibitory effect of a bioactive extract of |i|Funalia trogii|/i| (Berk.) Bond. et Singer, which was grown...
http://dl.begellhouse.com/fr/journals/708ae68d64b17c52,0d0f121956dd501b,36354e6b5da04bad.html
Semantic segmentation of HeLa cells: An objective comparison between one traditional algorithm and four deep-learning...Semantic segmentation of HeLa cells: An objective comparison between one traditional algorithm and four deep-learning...
The quantitative study of cell morphology is of great importance as the structure and condition of cells and their structures can be related to conditions of health or disease. The first step towards that, is the accurate segmentation of cell structures. In this work, we compare five approaches, one traditional and four deep-learning, for the semantic segmentation of the nuclear envelope of cervical cancer cells commonly known as HeLa cells. Images of a HeLa cancer cell were semantically segmented with one traditional image-processing algorithm and four three deep learning architectures: VGG16, ResNet18, Inception-ResNet-v2, and U-Net. Three hundred slices, each 2000 × 2000 pixels, of a HeLa Cell were acquired with Serial Block Face Scanning Electron Microscopy. The first three deep learning architectures were pre-trained with ImageNet and then fine-tuned with transfer learning. The U-Net architecture was trained from scratch with 36, 000 training images and labels of size 128 × 128. The ...
https://www.crick.ac.uk/research/publications/semantic-segmentation-of-hela-cells-an-objective-comparison-between-one-traditional-algorithm-and-four-deep-learning-architectures
Inhibition of Plasminogen Activator Inhibitor-1 | Arteriosclerosis, Thrombosis, and Vascular BiologyInhibition of Plasminogen Activator Inhibitor-1 | Arteriosclerosis, Thrombosis, and Vascular Biology
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
http://atvb.ahajournals.org/content/28/4/672.full
Inhibition of Plasminogen Activator Inhibitor-1 | Arteriosclerosis, Thrombosis, and Vascular BiologyInhibition of Plasminogen Activator Inhibitor-1 | Arteriosclerosis, Thrombosis, and Vascular Biology
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
http://atvb.ahajournals.org/content/28/4/672
Plus itPlus it
Cell and Culture Conditions. The PKR wild-type MEFs [MEF/PKR(+/+)] and PKR-knockout MEFs [MEF/PKR(-/-)], provided by Dr. Glen Barber (University of Miami, Miami, FL), have been described previously (Yang et al., 1995). The human cervical cancer cell line HeLa and the human lung cancer cell lines H1299, H460, H226B, and A549 were maintained in our laboratory. The human bronchial epithelial (HBE) cell line was purchased from Clonetics (Walkersville, MD). All cells except for HBE cells were routinely cultured in Dulbeccos modified Eagles medium supplemented with 10% heat-inactivated fetal calf serum, 100 units/ml penicillin, and 100 mg/ml streptomycin and maintained in the presence of 5% CO2 at 37°C. HBE cells were cultured in serum-free keratinocyte medium (Invitrogen, Carlsbad, CA).. Chemicals and Antibodies. A chemical library with 10,000 compounds, including BEPP and its analog BECC, was obtained from ChemBridge Research Laboratories, Inc.. The chemicals in the library were provided at a ...
http://jpet.aspetjournals.org/content/328/3/866
IncuCyte® HeLa NucLight Red Cells - IncuCyte eShopIncuCyte® HeLa NucLight Red Cells - IncuCyte eShop
One cryopreserved vial of HeLa NucLight Red (nuclear-labelled RFP) expressing cells at a density of 1x106 cells/vial. Each vial contains a stable population of 1 million HeLa cells expressing the NucLight Red fluorescent protein restricted to the nucleus. Parental HeLa cells were purchased from ATCC (Cat# CCL-2). HeLa
https://shop.essenbioscience.com/collections/nuclight-red-reagents/products/hela-nuclight-red-cells
Attachment of DNA to the nucleoskeleton of HeLa cells examined using physiological conditions. - Oxford Neuroscience
Although it is widely believed that eukaryotic DNA is looped by attachment to a nucleoskeleton, there is controversy about its composition and which sequences are attached to it. As most nuclear derivatives are isolated using unphysiological conditions, the criticism that attachments seen in vitro are generated artifactually has been difficult to rebut. Therefore we have re-investigated attachments of chromatin to the skeleton using physiological conditions. HeLa cells are encapsulated in agarose microbeads and lysed using Triton in a physiological buffer. Then, most chromatin can be electroeluted after treatment with a restriction enzyme to leave some at the base of the loops still attached. Analysis of the size and amounts of these residual fragments indicates that the loops are 80-90kbp long. The residual fragments are stably attached, with about 1kbp of each fragment protected from nuclease attack. This is very much longer than a typical protein-binding site of 10-20bp.
https://www.neuroscience.ox.ac.uk/publications/24848
Effects of miRNA-143 and the non-coding RNA MALAT1 on the pathogenesis and metastasis of HeLa cells. | GMR | Genetics and...Effects of miRNA-143 and the non-coding RNA MALAT1 on the pathogenesis and metastasis of HeLa cells. | GMR | Genetics and...
Cervical cancer is a common female malignancy of global dimensions. MicroRNAs (miRNAs) play crucial roles in the development, differentiation, proliferation, and apoptosis of tumors. The non-coding RNA MALAT1 participates in various physiological processes that are important for proper functioning of the body. Here, we analyzed the expression of miRNA-143 and MALAT1 in HeLa cells to evaluate their roles in the occurrence and metastasis of cervical cancer. HeLa cells were divided into five groups depending on the treatment conditions, namely, transfected with miRNA-143, MALAT1, miRNA-143 inhibitor and the MALAT1 inhibitor, and the untreated control. Reverse transcription-polymerase chain reaction was used to analyze the expression of miRNA-143 and MALAT1, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to assess proliferation, the trans-well assay to study cell invasion and migration, and western blot to analyze the levels of E-cadherin and vimentin. The proliferation of HeLa
https://geneticsmr.com/articles/effects-mirna-143-and-non-coding-rna-malat1-pathogenesis-and-metastasis-hela-cells
Isp for a dns server - Hosting Blog
While both the Rabenosyn-5 and Vps45 orthologs appear to coordinate endocytic regulatory functions in yeast and invertebrates, their relationship in mammalian cells is not well understood. Leaving out -L would be equivalent to L0, or specifying that the pool level was backup01. To assess the impact of TBC1d5 loss on VPS35 recruitment to endosomes, TBC1d5 KO cells were mixed with control HeLa cells at a ratio of 1:1 and the cells were simultaneously stained with TBC1d5, VPS35 and EEA1. 1 ). This approach, used by most system emulators, allows the emulator to run an arbitrary guest operating system without modifications because guest OS is not aware that it is not running on real hardware. This is consistent with a proposed role for the vps pathway in VP40 vesicular release. Untuk konsultasi konfigurasi server custom isp for a dns server mendapatkan diskon terbaik (untuk pembelian cloud resources dalam jumlah banyak), silahkan hubungi free terraria server download. BlueHost grew enormously in the ...
http://kelurahankemuning.info/for-server/isp-for-a-dns-server.html
On the spatial organization of ubiquitin-dependent proteolysis in HeLa cells<...On the spatial organization of ubiquitin-dependent proteolysis in HeLa cells<...
TY - JOUR. T1 - On the spatial organization of ubiquitin-dependent proteolysis in HeLa cells. AU - Wójcik, Cezary. PY - 1997/1/1. Y1 - 1997/1/1. KW - HeLa cells. KW - proteasome. KW - proteolysis. KW - ubiquitin. UR - http://www.scopus.com/inward/record.url?scp=0342941148&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0342941148&partnerID=8YFLogxK. M3 - Article. C2 - 9151102. AN - SCOPUS:0342941148. VL - 35. SP - 117. EP - 118. JO - Folia Histochemica et Cytochemica. JF - Folia Histochemica et Cytochemica. SN - 0015-5586. IS - 2. ER - ...
https://ohsu.pure.elsevier.com/en/publications/on-the-spatial-organization-of-ubiquitin-dependent-proteolysis-in-2
INA-UV induced apoptosis in Hela cells is also caspase | Open-iINA-UV induced apoptosis in Hela cells is also caspase | Open-i
INA-UV induced apoptosis in Hela cells is also caspase dependent. A. Hela cells, pre-incubated or not with 40 μM ZVADfmk were treated with indicated amounts of
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2666636_1471-2121-10-21-4&req=4
Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis | JCBUsp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis | JCB
HeLa cells were washed with cold PBS and lysed in cell lysis buffer (20-mM Tris-HCl, pH 8.0, 150-mM NaCl, 2-mM EGTA, 0.5-mM EDTA, 0.5% NP-40, 5-mM NaF, 1-mM Na3VO4, 1-mM PMSF, and 500× protease inhibitor cocktail; Calbiochem) for 20 min on ice. For Usp16/Plk1 coIP assays, cell lysates were mixed with anti-Usp16 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Usp16 1-257 aa) or mouse anti-Plk1 antibodies (06-813; EMD Millipore) and 15 µl protein A-Sepharose beads (75% slurry) and incubated at 4°C for 1 h. After washing with lysis buffer, the beads were harvested and suspended in Laemmli sample buffer. For Plk1/BubR1 coIP assays, cell lysates were mixed with anti-Plk1 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Plk1 326-end). For GST pull-down assays, HeLa cell lysates were incubated with 5 µg of soluble GST or GST-fused proteins bound to 15 µl glutathione-Sepharose beads (75% slurry) at 4°C for 1 h. After washing with lysis buffer, ...
http://jcb.rupress.org/content/210/5/727.full
Bitesize Bio: Top 5 of the most commonly used cell lines!Bitesize Bio: Top 5 of the most commonly used cell lines!
Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options. Just like model […]. The post Top 5 of the most commonly used cell lines! appeared first on Bitesize Bio.. ...
http://bitesizebiouk.blogspot.com/2017/04/top-5-of-most-commonly-used-cell-lines.html
TMX-KLH Antibody | Transcription | CapralogicsTMX-KLH Antibody | Transcription | Capralogics
AC0206: Western Blot (1:1000) of over-expressed TMX with positive and negative controls on HeLa Cells. TMX is the 34kDa band. The upper band (~38 kDa) is myc-tagged TMX. The lower band at~28 kDa (*) is a non-specific band.. ...
https://www.capralogics.com/antibody-products/transcription/tmx-klh
Purification and characterization of two forms of DNA polymerase alpha from HeLa cell nuclei. - PubMed - NCBIPurification and characterization of two forms of DNA polymerase alpha from HeLa cell nuclei. - PubMed - NCBI
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
https://www.ncbi.nlm.nih.gov/pubmed/6838843
Kinetics of B-fragment transport from EE/RE to the Golg | Open-iKinetics of B-fragment transport from EE/RE to the Golg | Open-i
Kinetics of B-fragment transport from EE/RE to the Golgi apparatus. (A) Confocal microscopy on living HeLa cells. Fluorophore-labeled B-fragment was internalize
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2132951_JCB9806018.f3a&req=4
A click-activated fluorescent probe for selective detection of hydrazoic acid and its application in biological imaging -...A click-activated fluorescent probe for selective detection of hydrazoic acid and its application in biological imaging -...
Here we present a new approach to aqueous hydrazoic acid detection through the synthesis and evaluation of an alkyne-based fluorescent probe, which could be applied in the monitoring of hydrazoic acid in both living Hela cells and larval zebrafish. This probe can also serve as an early warning automaton whic
http://pubs.rsc.org/en/Content/ArticleLanding/2013/CC/C3CC40698A?utm_source=feedburner&utm_medium=feed&utm_campaign=Feed%3A+rss%2FCC+%28RSC+-+Chem.+Commun.+latest+articles%29
热休克蛋白70反义脱氧寡核苷酸对宫颈癌HeLa细胞生长和凋亡及化学治疗敏感性的影响 · 临床与病理杂志热休克蛋白70反义脱氧寡核苷酸对宫颈癌HeLa细胞生长和凋亡及化学治疗敏感性的影响 · 临床与病理杂志
目的：探讨反义脱氧寡核苷酸封闭HSP70基因对体外宫颈癌 HeLa 细胞增殖、凋亡及化疗敏感性的影响。方法：1)将体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、反义寡核苷酸处理组(AS组)、正义寡核苷酸处理组(S组)、随机寡核苷酸处理组(R组)，每组各5例，分别转染体外培养的宫颈癌 HeLa 细胞，采用Western免疫印迹检测各组细胞HSP70蛋白表达。2)将顺铂处理体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、单纯顺铂处理组(Cis组)、反义寡核苷酸+顺铂处理组(AS +Cis组)、正义寡核苷酸+顺铂处理组(S +Cis组)、随机寡核苷酸+顺铂处理组 (R +Cis组)，每组各8例。采用四甲基偶氮唑蓝光吸收法(methyl thiazolyl tetrazolium，MTT) 法检测 HeLa 细胞的生长抑制率; 流式细胞术检测 HeLa 细胞的凋亡率。结果：1)Ctrl组、AS组、S组、R组HSP70灰度比值分别为1.365±0.187，0.379±0.134，1.403±0.163和1.410±0
http://lcbl.amegroups.com/articles/70
The good, the bad, and the HeLa - The Berkeley Science ReviewThe good, the bad, and the HeLa - The Berkeley Science Review
When Professor Gertrude Buehring steps into her lab in Koshland Hall, she is never met by HeLa cells. In fact, she makes a point of it. We never grow them, she says. I wouldnt want to take that risk, actually.. Buehring, a professor of virology in the School of Public Health, has a reason to be wary of HeLa. Both her PhD and postdoc careers were spent working at UC Berkeleys Cell Culture Laboratory housed in the Naval Biosciences Laboratory in Oakland, a cell repository funded by the federal government that characterized and maintained cell lines for research scientists. She happened to be working there at a time when Dr. Walter Nelson-Rees, the co-director, was working hard to expose HeLas misdeeds. The vigorous cells crime? The contamination of other, less hardy cell lines.. Nelson-Rees was not the first to suspect contamination by HeLa cells. In the 1960s, Dr. Stanley Gartler, a research geneticist, released the initial HeLa bomb. Gartler had discovered that the 18 different cell ...
https://berkeleysciencereview.com/article/good-bad-hela/
Considerations of Cell Counting Analysis when using Different Types of CellsConsiderations of Cell Counting Analysis when using Different Types of Cells
This paper reviews several different cell types and cell preparation approaches using standard cells, such as, CHO cells, EL4 cells, SF9 cells, and HELA cell cultures.
https://www.mybeckman.in/resources/reading-material/application-notes/considerations-of-cell-counting-analysis-when-using-different-types-of-cells
RanBPM shRNA HeLa cell line as a research tool - WORLDiscoveries® | Business Development Office | London, ONRanBPM shRNA HeLa cell line as a research tool - WORLDiscoveries® | Business Development Office | London, ON
Ran-Binding Protein M (RanBPM) has been previously shown to inhibit c-Raf expression, however how this was achieved remains unclear. c-Raf is the central component of the extracellular signal-regulated kinase (ERK) pathway which has been linked to many cancer types. Furthermore, RanBPM was recently identified as part of the E3 ubiquitin ligase complex and the CTLH (C-terminal to LisH) complex (McTavish et al., 2019). Lastly, RanBPM has been linked to various signaling pathways related to numerous cellular processes which include - apoptosis, cell adhesion, migration, transcription, nuclear-cytoplasmic transport and also plays a significant role during development (Salemi et al., 2017). ...
https://worldiscoveries.ca/technologies/ranbpm-shrna-hela-cell-line-as-a-research-tool-2/
IFITM1 Knockout HeLa Cell Lysate (NBP2-65153): Novus BiologicalsIFITM1 Knockout HeLa Cell Lysate (NBP2-65153): Novus Biologicals
Human IFITM1 Knockout HeLa Cell Lysate parental control lysate. CRISPR/Cas9 generated negative control for antibody validation in WB.
https://www.novusbio.com/products/ifitm1-lysate_nbp2-65153
The assembly of a rotating HeLa cell/gold rod aggregate at an acoustic nodal line in xy plane. | Penn State UniversityThe assembly of a rotating HeLa cell/gold rod aggregate at an acoustic nodal line in xy plane. | Penn State University
The assembly of a rotating HeLa cell/gold rod aggregate at an acoustic nodal line in the xy plane. The video was taken under 500X overall magnification except for 00:23 - 00:32 and 01:16 - 01:42, where a 200X overall magnification was used.
http://news.psu.edu/video/303317/2014/02/10/assembly-rotating-hela-cellgold-rod-aggregate-acoustic-nodal-line-xy-plane
Anomalous structures in the cytoplasm of HeLa cells cultured in the presence of 5-bromodeoxvuridine.<...
TY - JOUR. T1 - Anomalous structures in the cytoplasm of HeLa cells cultured in the presence of 5-bromodeoxvuridine.. AU - HENDEE, W. R.. AU - ZEBRUN, W.. AU - BONTE, F. J.. PY - 1963/6. Y1 - 1963/6. UR - http://www.scopus.com/inward/record.url?scp=84883840494&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84883840494&partnerID=8YFLogxK. M3 - Article. C2 - 13953692. AN - SCOPUS:84883840494. VL - 17. SP - 675. EP - 680. JO - Journal of Cell Biology. JF - Journal of Cell Biology. SN - 0021-9525. ER - ...
https://utsouthwestern.pure.elsevier.com/en/publications/anomalous-structures-in-the-cytoplasm-of-hela-cells-cultured-in-t
RNA in HeLa cell - Cell BiologyRNA in HeLa cell - Cell Biology
Could anyone tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment.Thanks a lot!. ...
http://www.protocol-online.org/biology-forums/posts/27955.html
Cytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated PathwayCytotoxicity of ICD-85 NPs on Human Cervical Carcinoma HeLa Cells through Caspase-8 Mediated Pathway
The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cytotoxicity against HeLa cellscompared to the free ICD-85. The IC50 value at 72 h was reduced from 25 ± 2.9 μg/mL for free ICD-85 to 15.5 ± 2.4 μg/mL for ICD-85 NPs. However, LDH assay demonstrated that ICD-85 has dose-dependent cytotoxicity on HeLa cells while ICD-85 NPs exhibited weaker cytotoxicity on same cells. The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. Moreover, caspase-8 assay analysis demonstrated that the ICD-85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. Our results demonstrated that the
http://ijpr.sbmu.ac.ir/article_1253.html
Magiran | The effect of Lactobacillus brevis on Apoptosis and casp (casp8, casp3) gene Expression in HeLa Cancer CellsMagiran | The effect of Lactobacillus brevis on Apoptosis and casp (casp8, casp3) gene Expression in HeLa Cancer Cells
Background and AimCervical and breast cancer are considered to be the most common cause of death among Iranian women. Various studies have shown that probiotics are effective fighting cancer. The aim of the present study was to investigate the effect of Lactobacillus brevis on apoptosis and casp (casp3, casp8) gene expression in HeLa cancer cells. Materials and MethodsIn this study, L. brevis bacteria were collected from the vaginal fluid of females referred to Alzahra Hospital and isolated and cultured in MRS agar medium. Inhibition of HeLa cancer cell proliferation by bacteria was evaluated by MTT assay. Apoptosis of cancer cells was measured by fluorescent microscopy using DAPI method. Finally, expression of Caspase and akt genes was measured by Real time PCR.ResultsThe results indicated that L. brevis had the same effect on HeLa cancer cells (P=0.42, P=0.26). On the other hand, there was no significant increase in akt gene expression (p> 0.05).ConclusionLactobacillus brevis bacteria can be
https://www.magiran.com/paper/2104276/?lang=en
Knock-down of NDRG2 sensitizes cervical cancer Hela cells to cisplatin through suppressing Bcl-2 expression | BMC Cancer | Full...Knock-down of NDRG2 sensitizes cervical cancer Hela cells to cisplatin through suppressing Bcl-2 expression | BMC Cancer | Full...
NDRG2, a member of N-Myc downstream regulated gene family, plays some roles in cellular stress, cell differentiation and tumor suppression. We have found that NDRG2 expression in cervical cancer Hela cells increases significantly upon stimulation with cisplatin, the most popular chemotherapeutic agent currently used for the treatment of advanced cervical cancer. This interesting phenomenon drove us to evaluate the role of NDRG2 in chemosensitivity of Hela cells. In the present study, RNA interference was employed to down-regulate NDRG2 expression in Hela cells. RT-PCR and Western blot were used to detect expression of NDRG2, Bcl-2 and Bax in cancer cells. Real-time PCR was applied to detect miR-15b and miR-16 expression levels. Drug sensitivity was determined with MTT assay. Cell cloning efficiency was evaluated by Colony-forming assay. Apoptotic cells were detected with annexin V staining and flow cytometry. In vitro drug sensitivity assay revealed that suppression of NDRG2 could sensitize Hela cells
https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-370
Archives of Clinical Infectious Diseases | Oxamflatin Induces E-cadherin Expression in HeLa Cervical Carcinoma CellsArchives of Clinical Infectious Diseases | Oxamflatin Induces E-cadherin Expression in HeLa Cervical Carcinoma Cells
Epithelial-to-mesenchymal transition (EMT) renders cervical cancer cells more susceptible to metastasis, and reduced expression levels of E-cadherin have been shown in this malignancy (9). E-cadherin is a pivotal molecule involved in epithelial cell adhesion, aberrant expression of which has been demonstrated in various human malignancies as a result of irreversible (genetic) and reversible (epigenetic) modifications of the E-cadherin gene (CDH1), such that the epigenetic ones have been more implicated in E-cadherin downregulation (10).. In this study, we evaluated the impact of oxamflatin on HeLa cell line by determining the expression level of E-cadherin. Our results demonstrated that oxamflatin treatment leads to a time- and concentration-dependent increase in E-cadherin expression in cervical cancer cell lines.. There is ample evidence reporting that HDACIs could be applied to re-express silenced genes (9, 11). For example, valproic acid (VPA), as a HDAC inhibitor, led to the re-expression ...
http://archcid.com/en/articles/66977.html
Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting<...
TY - JOUR. T1 - Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting. AU - Yamahara, Yumi. AU - Nagasawa, Naotsugu. AU - Taguchi, Mitsumasa. AU - Oshima, Akihiro. AU - Washio, Masakazu. PY - 2018/1. Y1 - 2018/1. N2 - Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is ...
https://waseda.pure.elsevier.com/en/publications/fabrication-of-thermo-responsive-pnipaam-g-etfe-for-cell-culture-
Determination of Antineoplastic Activity of Rohituka, Aphanamixis Polystachya (Wall) RN Parker in Hela Cells: Correlation with...Determination of Antineoplastic Activity of Rohituka, Aphanamixis Polystachya (Wall) RN Parker in Hela Cells: Correlation with...
The antineoplastic activity of chloroform stem bark extract of rohituka, Aphanamixis polystachya (APE) used traditionally to treat spleen and liver tumors was evaluated in cultured HeLa cells by clonogenic and micronucleus assays. Treatment of HeLa cells with 0, 5, 10, 25, 50, 75 or 100µg/ml APE for different times caused a concentration-dependent reduction in the cell survival up to 6 h post-treatment (p|0.005) followed by a non-significant decline in the cells treated with APE for 24 h. Therefore, 6h treatment time was considered as an optimum time for APE exposure and further studies were carried out using this treatment time. Exposure of HeLa cells to different concentrations of APE resulted in a concentration-dependent decline in the cell viability and 100µg/ ml APE resulted in 72% cell death when compared with the non-drug treated control group. These results were further confirmed by clonogenic assay where, APE treatment caused a concentration-dependent decline in the clonogenicity of HeLa
https://medcraveonline.com/IJCAM/IJCAM-03-00083.php
Cytotoxic and pro-apoptotic effects of selected indigenous medicinal plants on cervical cancer cell line (Hela cells)Cytotoxic and pro-apoptotic effects of selected indigenous medicinal plants on cervical cancer cell line (Hela cells)
Carcinoma of the cervix is the second most common cancer among women worldwide especially in the developing world. Due to severe reported cytotoxic tendenciesby anticancer drugs, newer therapies from natural products are desirable. This work therefore aims to partially purify pro-apoptotic agents from indigenous natural products and to investigate the mechanism of action of the selected plant fractions.Sixteen indigenous medicinal plants were collected from South West Nigeria. Plant extracts were fractionated into hexane (HF), chloroform (CF), ethylacetate (EAF), methanol (MF) and water (WF) fractions using solvent-solvent partitioning and polyamide adsorption chromatography. The resultant ninety-six test fractions were screened for lethality potential using brine shrimp lethality (BSL) assay. Fractions with LC50 less than 10 ug/ml were subjected to Water Soluble Tetrazolium (WST-1) cytotoxicity assay and apoptosis evaluations using: morphological assessment, flow cytometric analysis of ...
https://ir.unilag.edu.ng/handle/123456789/6098
The m 6 A eraser FTO facilitates proliferation and migration of human cervical cancer cells | Cancer Cell International | Full...The m 6 A eraser FTO facilitates proliferation and migration of human cervical cancer cells | Cancer Cell International | Full...
Since FTO was recognized as the first m6A demethylase, the understanding of its biological function has been widely expanded. However, the role of FTO in cervical cancer tumorigenesis remains unclear. In this study, we first analyzed the expression of FTO in two independent human cancer datasets and evaluated the correlation between FTO level and cervical cancer progression. Using small hairpin RNA technology, we explored the function of FTO in cervical cancer cell line Hela and SiHa cells, respectively. We then determined the FTO targets by performing transcriptional profile with FTO deficient and competent Hela cells, and finally validated these targets with ribosome profiling and functional rescue experiments. Our data suggested that FTO was frequently overexpressed in human cervical cancer tissues and highly correlated with cervical cancer progression. FTO serves as an oncogenic regulator for cervical cancer cells proliferation and migration which is vastly depended on its demethylase activity.
https://cancerci.biomedcentral.com/articles/10.1186/s12935-019-1045-1/email/correspondent/c2/new
Electrical characterization of single HeLa cell using 2D simulation and spectroscopy measurement<...
TY - GEN. T1 - Electrical characterization of single HeLa cell using 2D simulation and spectroscopy measurement. AU - Wang, M. H.. AU - Chen, M. K.. AU - Jang, L. S.. PY - 2008/12/1. Y1 - 2008/12/1. N2 - The electrical properties of single cells provide fundamental insights into their pathological condition and are therefore of immense interest to medical practitioners. Accordingly, this study captures single HeLa cells using a microfluidic device and then measures their impedance properties using a commercial impedance spectroscopy system. The experimental system is modeled by an equivalent electrical circuit and COMSOL simulations are then performed to establish the conductivity, permittivity and impedance of single HeLa cells under various operational frequencies and voltages. At an operational voltage of 0.2 V, the maximum deviation between the experimental and simulation results for the magnitude and phase of the HeLa cell impedance is found to be 9.5% and 4.2%, respectively. In general, ...
https://researchoutput.ncku.edu.tw/en/publications/electrical-characterization-of-single-hela-cell-using-2d-simulati
Plus itPlus it
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a marked stimulation of inorganic [32P]orthophosphate incorporation into HeLa-cell phosphatidylcholine (PC), phosphatidylethanolamine (PE), and lysophosphatidylethanolamine. The increased incorporation of inorganic [32P]orthophosphate into PE and lysophosphatidylethanolamine in the presence of TPA was not associated with an increase in PE synthesis as detected by the incorporation of [3H]serine or [3H]ethanolamine. The PC-specific exchange protein from beef liver was used to insert PC labeled with [3H]choline, inorganic [32P]orthophosphate, or [14C]arachidonic acid plus [3H]palmitic acid into the outer monolayer of intact HeLa cell membranes. Radioactivity from the latter two compounds was rapidly incorporated into PE and lysophosphatidylethanolamine; the incorporation was stimulated by TPA. It was concluded that TPA stimulated the formation of PE by base exchange between ethanolamine and PC.. ...
http://cancerres.aacrjournals.org/content/43/11/5564
Are stem cell lines cryogenically preserved or commercially available like HeLa cells are? - Biology Stack ExchangeAre stem cell lines cryogenically preserved or commercially available like HeLa cells are? - Biology Stack Exchange
This is too long for a comment, so I have to write it in here:. Mostly this is because other cell lines are more practical in the lab.. Stem cells are much more tricky to maintain - especially if you want to keep their stem cell properties. They only grow very slow, tend to differentiate when they get too much stress, cellular signals and so on and need special media (these need to be defined, so that they are not containing factors which promote differentiation for example). For everyday experiments (like expression profiles, experiments on the effects of certain substances on the growth, gene expression or whatever of cells) normal cell lines are much more suited as these are much easier to keep in the lab using standard media. Additionally,experiments can be more easily repeated and reproduced, since these cells should behave always the same.. ...
https://biology.stackexchange.com/questions/31637/are-stem-cell-lines-cryogenically-preserved-or-commercially-available-like-hela
skloot - Terra Sigillataskloot - Terra Sigillata
Twenty years ago this morning, I had to defend a body of work that contained this paragraph on page 24: HeLa cells are a human cervical carcinoma cell line having a doubling time of 24 hr and were obtained from Dr. Bert Flanegan, Dept. of Immunology and Microbiology, University of Florida. HeLa cells were maintained…. ...
http://scienceblogs.com/terrasig/tag/skloot/
Syntheses, crystal structure and cytotoxicity of diamine platinum(II) complexes containing maltol. - DROSyntheses, crystal structure and cytotoxicity of diamine platinum(II) complexes containing maltol. - DRO
The cationic complexes (1,2-diaminoethane)(maltolato)platinum(II) ([Pt(en)(ma)]+) and (1R,2R-1,2-diaminocyclohexane)(maltolato)platinum(II) ([Pt(R,R-DACH)(ma)]+) have been prepared and the structure of [Pt(R,R-DACH)(ma)]NO3 has been determined by single crystal X-ray diffraction. The geometry of the metal in [Pt(R,R-DACH)(ma)]NO3 is essentially square planar and the maltolate ligand has a geometry similar to other chelate complexes involving this ligand. The cytotoxicities of the compounds have been assessed in the human cell lines HeLa and K562 and the IC50 values are approximately 32 microM in HeLa cells and 26 microM in K562 cells. In these cell lines the cytotoxicity of cisplatin is higher than the maltolate complexes by a factor of 2 to 3 whereas the cytotoxicity of carboplatin is lower than the maltolate complexes ...
http://dro.deakin.edu.au/view/DU:30102731
Fractions of HeLa DNA differing in their content of guanine + cytosine<...Fractions of HeLa DNA differing in their content of guanine + cytosine<...
TY - JOUR. T1 - Fractions of HeLa DNA differing in their content of guanine + cytosine. AU - Schildkraut, Carl L.. AU - Maio, Joseph J.. PY - 1969/12/14. Y1 - 1969/12/14. N2 - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C contents according to the relation: ρ{variant} = 0.98 (G + C) + 1.659, where ρ{variant} refers to buoyant density and (G + C) to the average mole fraction of guanine + cytosine. This nucleolar DNA is significantly enriched with respect to two satellite bands: a light satellite (ρ{variant} = 1.686 g/ml.) and a heavy satellite (ρ{variant} = 1.712 g/ml.). In an alkaline CsCl density gradient, the light satellite DNA can be separated into two complementary strands, as demonstrated by base composition analysis.. AB - DNA isolated from preparations of HeLa cell nucleoli has been fractionated into six components which band in CsCl at densities corresponding to their G + C ...
https://einstein.pure.elsevier.com/en/publications/fractions-of-hela-dna-differing-in-their-content-of-guanine-cytos-2
HeLa whole cell lysate (Positive control) - NB800-PC1 | acris-antibodies.com
HeLa whole cell lysate (Positive control), 0.1 mg. This whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.
https://www.acris-antibodies.com/lysates/hela-whole-cell-lysate-positive-control-nb800-pc1.htm
HeLa whole cell lysate (ab29545) | アブカムHeLa whole cell lysate (ab29545) | アブカム
HeLa whole cell lysate Lysates datasheet (ab29545). Abcam offers quality products including antibodies, assays and other reagents.
https://www.abcam.co.jp/hela-whole-cell-lysate-ab29545.html
Plus itPlus it
The Mab K1 was generated by immunization of mice with the human ovarian carcinoma cell line OVCAR-3 (3) . The reactivity of Mab K1 against a variety of different human tumor cell lines was tested using immunofluorescence (3) . It showed reactivity with several ovarian cancer cell lines, including OVCAR-2, OVCAR-3, OVCAR-5, A-1847, and SKOV3; cervical cancer cell lines HeLa and KB; and gastric cell lines AGS and HTB103. No reactivity was detected with breast, colon, or prostate cancer cell lines. Care must be taken with the use of Mab K1 because its reactivity is acid labile (5) .. The reactivity of Mab K1 against normal human tissues was tested by immunohistochemistry using cryostat tissue sections (3) . Most normal tissues showed no reactivity with Mab K1, with the exception of mesothelial cells that line the peritoneal, pleural, and pericardial cavities. There was also weak reactivity with the basal cells of the trachea and cells in the Fallopian tubes. A similar immunoreactivity was seen in ...
https://clincancerres.aacrjournals.org/content/10/12/3937
SearchSearch
The relationships between DNA damage from UV radiation, alkylating drugs and the methylated xanthines (MX) have been studied in normal and malignant rodent and human cells. A comparison of the level of DNA excision repair (repair replication and unscheduled DNA synthesis) confirms that some forms of alkylating-agent damage (probably mono-filar DNA adducts) are less completely removed by both normal and malignant rodent cells than by their human counterparts, rendering rodent cells more susceptible to the toxic potential of unexcised lesions. The toxicity of alkylating agents can be increased by the presence of several MXs during the period of DNA replication which follows infliction of the damage. Human cells appear capable of excising more DNA damage, rendering them somewhat less susceptible to enhancement of cytotoxicity by MX. This resistance of human cells is only quantitative, however, since 2 human cancer cell lines (HeLa and HT-29) could be sensitized to a variety of alkylating agents by ...
https://escholarship.org/search/?q=author%3A%22Kulhanian%2C%20F%22
Supplementary Figure 1: MicroRNA represses target proteins without causing mRNA decay. | Nature Structural & Molecular BiologySupplementary Figure 1: MicroRNA represses target proteins without causing mRNA decay. | Nature Structural & Molecular Biology
a,b, Western blotting analysis of miR-20a target genes in response to increasing doses of miR-20a mimic (a) or antagomir (b). c,d, RT-qPCR analysis of miR-20a levels in HeLa cells transfected with miR-20a mimic (c) or antagomir (d). e,f, RT-qPCR analysis of DAPK3 mRNA levels in HeLa cells transfected with miR-20a mimic (e) or antagomir (f). g, Luciferase reporter constructs containing the miR-20a MRE from DAPK3 (native) in Renilla 5 UTR or mutated to restore base-pairing in the 5 end (seed) coupled with progressive mismatches (3MM+seed) in the 3 end. h,i, Results of the luciferase reporter assays (h) and quantification of luciferase mRNA (i). j,k, mRNA levels of luciferase reporters containing individual MREs from 4 indicated genes that function in both CDS and 3UTR (j) or those that function only in CDS (k), as shown in main Fig. 2e,f. l,m, Luciferase activities from the reporters containing individual MREs from 4 indicated genes that function only in CDS in response to co-transfected ...
https://www.nature.com/articles/s41594-018-0136-3/figures/7?error=cookies_not_supported&code=4c63d8fb-f259-43f4-afde-ad92257085c6
monolayermonolayer
We have been using HeLa cells in my laboratory since 1982, when I arrived at Columbia University Medical Center fresh from postdoctoral work with David Baltimore at MIT. I brought with me a line of HeLa cells and used them for 30 years for our research on viruses. Here is a story of how we lost the cells and then found them ten months later.. As everyone knows, the continuous HeLa cell line was derived from a cervical tumor taken from Henrietta Lacks in 1951 (if you dont know the story, you should read Rebecca Skloots The Immortal Life of Henrietta Lacks, or my shorter summary). When I arrived at the Baltimore lab in 1979, they were using cells derived from the S3 clone of HeLa cells that had been produced by Philip Marcus in the 1950s. I write derived from because someone at MIT had further cloned the S3 line and selected one that was particularly susceptible and permissive to poliovirus infection. This was the cell line that I took with me to Columbia in 1982.. Because we use so many HeLa ...
http://www.virology.ws/tag/monolayer/
Biology Professor Publishes Solo Article and One with Recent Alumna \ Westminster CollegeBiology Professor Publishes Solo Article and One with Recent Alumna \ Westminster College
Dr. Karen Resendes, Westminster College assistant professor of biology, recently published two articles on HeLa cell research, one of which was in collaboration with Gretchen Diffendall 14.
http://www.westminster.edu/about/news/release.cfm?id=8292
Browsing University Honors Capstone Projects by Subject radioprotectors
In this study, the cell lines HeLa, 3T3-Swiss albino, CHO-Kl, and ARPE-19 were cultured and co-incubated with variable concentrations of S-phosphorylated thiols and Nacetylcysteine. Using MTT assays, the cultures were ...
https://commons.lib.niu.edu/handle/10843/13631/browse?type=subject&value=radioprotectors
Versuche zum Calciphylaxie-Phänomen an HeLa-Zellkulturen | SpringerLinkVersuche zum Calciphylaxie-Phänomen an HeLa-Zellkulturen | SpringerLink
An attempt was made to induce calciphylaxis and direct calcifying reaction according to Selye2in HeLa cell cultures. With our experimental conditions, we obtained neither a calciphylaxis nor a...
https://link.springer.com/article/10.1007/BF01897419
Difference between revisions of Cell Culturing - OpenWetWareDifference between revisions of Cell Culturing - OpenWetWare
Derived from Henrietta Lacks in 1951, HeLa is an immortal cell line that is useful for its remarkable prolificity and durability. It was the inspiration for the national bestseller, The Immortal Life of Henrietta Lacks, written by the incredibly attractive Rebecca Skloots. Because of their high reproductive rate, HeLa cells are a good beginner transfection cell line to test the efficacy of the CPNP delivery of the origami. ...
https://openwetware.org/wiki/?title=Cell_Culturing&curid=134134&diff=0&oldid=696800
antioxidantsantioxidants
In the second of three interviews with Bill Andrews at the 2016 AMMG, we discuss the Henrietta Lacks (or HeLa) cervical cancer cells commonly used in cell research. Dr. Andrews also discusses why telomere erosion is less a factor in mice aging.. ...
https://www.rechargebiomedical.com/tag/antioxidants/
Omics! Omics!: Time to Retire HeLa?Omics! Omics!: Time to Retire HeLa?
The final argument Ill advance against HeLa in cancer research is the fact that it has been in culture so long. HeLa should be seen as something like a bizarre new species found only in laboratories, not a representative of human cancer. All that time in culture has certainly enabled HeLa (or all the different HeLa) to evolve far away from the tumor which killed Henrietta Lacks. Again, this is a flaw in all in vitro propagated cell line studies, but given that HeLa is the oldest it is likely the most affected (granted, what wed really like to know is actual time in culture). Patient-derived xenografts are far more likely to be informative of tumors, but their expense and newness has meant they havent yet fully displaced in vitro lines from research programs ...
https://omicsomics.blogspot.com/2017/04/time-to-retire-hela.html
Omics! Omics!: Time to Retire HeLa?
The final argument Ill advance against HeLa in cancer research is the fact that it has been in culture so long. HeLa should be seen as something like a bizarre new species found only in laboratories, not a representative of human cancer. All that time in culture has certainly enabled HeLa (or all the different HeLa) to evolve far away from the tumor which killed Henrietta Lacks. Again, this is a flaw in all in vitro propagated cell line studies, but given that HeLa is the oldest it is likely the most affected (granted, what wed really like to know is actual time in culture). Patient-derived xenografts are far more likely to be informative of tumors, but their expense and newness has meant they havent yet fully displaced in vitro lines from research programs ...
http://omicsomics.blogspot.co.uk/2017/04/time-to-retire-hela.html
Protein Phosphatase 2A-Dependent Dephosphorylation of Replication Protein A Is Required for the Repair of DNA Breaks Induced by...Protein Phosphatase 2A-Dependent Dephosphorylation of Replication Protein A Is Required for the Repair of DNA Breaks Induced by...
FIG. 2. PP2A mediates RPA32 dephosphorylation at T21 and S33. (A) HeLa cells were transfected with a mock siRNA oligonucleotide or with oligonucleotides against the catalytic subunits of PP1, PP2A, PP4, PP5, and PP6. Twenty-four hours posttransfection, cells were pulse treated with HU (0.2 mM, 24 h), and RPA32 dephosphorylation at T21/S33 was compared at the indicated time points. (B) HeLa cells transfected with control siRNA or siRNA against PP2A/C were pulse treated with HU (0.2 mM, 24 h). At 0 to 12 h postrelease, the cells were fixed and costained with DAPI and anti-RPA32pS33 and anti-γ-H2AX antibodies. The DNA damage-inducible foci were visualized by immunofluorescence microscopy, and the percentage of phospho-RPA32 and γ-H2AX focus-positive cells was determined, normalized, and plotted. The error bars represent the standard deviations from three independent experiments. (C) Immunoprecipitation (IP) assays were performed in unperturbed cells or cells recovering from exposure to pulses of ...
https://mcb.asm.org/content/29/21/5696/figures-only
CIL:13731, Homo sapiens, endothelial cell. CIL. DatasetCIL:13731, Homo sapiens, endothelial cell. CIL. Dataset
Mitochondria in HeLa cells are identified by mito-YFP (green) following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-c...
http://ccdb.ucsd.edu/images/13731
HeLa Cells with Dil | MicroscopyUHeLa Cells with Dil | MicroscopyU
This section illustrates a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye, Dil, which targets membranes. The absorption maximum of Dil is 549 nanometers and the emission maximum occurs at 565 nanometers (in the yellow region of the visible light spectrum). Fluorescence intensity arising from labeled cytoskeletal membrane components is quite evident in the image even through the probe emission peak is 30 nanometers below the Y-2E/C filter set dichromatic mirror cut-on wavelength.
https://www.microscopyu.com/gallery-images/hela-cells-with-dil
Cell-biology] Proteins secreted by HeLa cellsCell-biology] Proteins secreted by HeLa cells
hi i,m rodrigo from chile and i have the same question of which proteins can be secreted by HeLa cells into the growth medium, if you know the answer please send to me a this mail rodrigosakuragi from gmail.com ...
http://www.bio.net/bionet/mm/cellbiol/2006-October/015838.html
Hela Cells Essay
However, the HeLa cells have also caused much pain, suffering, and confusion for the Lacks family. The Lackss family, for example, saw no profits despite being asked to contribute blood for subsequent research about which they were not adequately informed. Henrietta Lacks was treated during a time when medical ethics were very different.
https://qefafacezuwizyfuq.tsfutbol.com/hela-cells-essay-11227jm.html
Hela Difluo Invivogen - GenopriceHela Difluo Invivogen - Genoprice
Lab Reagents Human IgG antibody Laboratories manufactures the hela difluo invivogen reagents distributed by Genprice. The Hela Difluo Invivogen reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact InVivoGen. Other Hela products are available in stock. Specificity: Hela Category: Difluo Group: Invivogen Invivogen information ...
https://genediag.com/hela-difluo-invivogen/
JCI - WelcomeJCI - Welcome
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
https://jci.org/tags/32
JCI - WelcomeJCI - Welcome
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
https://m.jci.org/tags/32
JCI - WelcomeJCI - Welcome
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
https://haochuduo.net.insight.mobile.jci.org/tags/32
Expression profiling of HeLa cell line treated with CDK9 inhibitor | bioCADDIE Data Discovery IndexExpression profiling of HeLa cell line treated with CDK9 inhibitor | bioCADDIE Data Discovery Index
DataMed is a prototype biomedical data search engine. Its goal is to discover data sets across data repositories or data aggregators. In the future it will allow searching outside these boundaries. DataMed supports the NIH-endorsed FAIR principles of Findability, Accessibility, Interoperability and Reusability of datasets with current functionality assisting in finding datasets and providing access information about them.
https://datamed.org/dataset/0006/5913a12b5152c62a9fc1a199/Expression-profiling-of-HeLa-cell-line-treated-with-CDK9-inhibitor
hACE2-HeLa Stable Cell Line - Creative Biolabs
Human ACE2 gene was integrated into the chromosomes of HeLa cells to form an engineered cell line, hACE2-HeLa, that stably expresses ACE2 on the cell surface.
https://sars-cov-2.creative-biolabs.com/hace2-hela-stable-cell-line-2455.htm
CIL:17448, Homo sapiens, cervical carcinoma. CIL. Dataset
Fluorescence images of control and Si-RNA transfected HeLa cells treated with nocodazole and allowed to recover. Mitotic cells were stained for alpha-...
http://cellimagelibrary.org/images/17448
Immortal Life of Henrietta Lacks (Prebound) | BookWoman
Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells--taken without her knowledge--became one of the most important tools in medicine. The first immortal human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. If you could pile all HeLa cells ever grown onto a scale, theyd weigh more than 50 million metric tons--as much as a hundred Empire State Buildings. HeLa cells were vital for developing the polio vaccine; uncovered secrets of cancer, viruses, and the atom bombs effects; helped lead to important advances like in vitro fertilization, cloning, and gene mapping; and have been bought and sold by the billions ...
http://www.ebookwoman.com/book/9781613831199
Genetics | A Schooner of Science - Part 2Genetics | A Schooner of Science - Part 2
comes out next month, written about the woman and the cells which should have made her famous. Rebecca Skloot been researching it for something like 10 years and its got some great reviews. Im going to pre-order a copy, and if youd like to know more about HeLa cells and Henrietta Lacks, do the same! Its a story that deserves to be heard, and if there are enough pre-orders, Amazon will help promote the book. Plus its 30% off at the moment. What more could you want? Heres the blurb.. Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells-taken without her knowledge-became one of the most important tools in medicine. The first immortal human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. If you could pile all HeLa cells ever grown onto a scale, theyd weigh more than 50 million metric tons-as much as a hundred Empire State ...
http://aschoonerofscience.com/tag/genetics/page/2/
Role of PKR in the Inhibition of Proliferation and Translation by Polycystin-1 : Figure 1Role of PKR in the Inhibition of Proliferation and Translation by Polycystin-1 : Figure 1
Figure 1: Effects of PKR on the proliferation and translation. (a) Effects of PKR on the proliferation of HeLa cells. After being transfected with plasmids PKR, PKR siRNA, or GFP, HeLa cells were plated in multiple wells of a 96-well plate and grown for 24 hr for cell proliferation assays. Cells from the sample preparations were collected for immunoblotting. Proliferation rate of the control sample was normalized to 100%. PKR, WT PKR; si-PKR, PKR siRNA; Ctrl, GFP. Upper panel, averaged data (N=4 ...
https://www.hindawi.com/journals/bmri/2019/5320747/fig1/
UV-treated HeLa Lysates ab157396 | AbcamUV-treated HeLa Lysates ab157396 | Abcam
Lysates of HeLa cells treated with UV or untreated cells for use as controls in western blotting to analyze UV-induced DNA damage and/or apoptosis.
http://www.abcam.com/hela-dna-damage-whole-cell-lysate-set-uv-treated-and-untreated-control-ab157396.html
HeLa, the first immortal human cells and a tale of immorality | A Schooner of ScienceHeLa, the first immortal human cells and a tale of immorality | A Schooner of Science
When we work with cell lines in the lab, we often work with HeLa cells. They can live in a vial of nutrients, and from a small sample you can grow a large
https://aschoonerofscience.com/unethics/hela-the-first-immortal-human-cells-and-a-tale-of-immorality/
Human Cell Cycle : Hela Cells | Home
Within this site you can view figures from the papers, supplementary data, and interactively explore the cell cycle dataset. In addition, raw data and processed data are available for download.. ...
http://genome-www.stanford.edu/Human-CellCycle/Hela/
BUB1BUB1
In vitro knockdown of Bub1 in p53 impaired cells (e.g. HeLa cells) caused aneuploidy. Whether aneuploidy alone is a sufficient ... Cell. 32 (3): 394-405. doi:10.1016/j.molcel.2008.09.017. PMC 2644263. PMID 18995837. UniProt: O43683 "BUB1 protein (Homo ... Jeganathan K, Malureanu L, Baker DJ, Abraham SC, van Deursen JM (Oct 2007). "Bub1 mediates cell death in response to chromosome ... Yu H (Dec 2002). "Regulation of APC-Cdc20 by the spindle checkpoint". Current Opinion in Cell Biology. 14 (6): 706-14. doi: ...
https://en.wikipedia.org/wiki/BUB1
List of Stuff You Should Know episodes (2013)List of Stuff You Should Know episodes (2013)
How HeLa Cells Work. HowStuffWorks. Retrieved 2016-03-17. How Underwater Tunnels Work. HowStuffWorks. Retrieved 2016-03-17. How ...
https://en.wikipedia.org/wiki/List_of_Stuff_You_Should_Know_episodes_(2013)
BlennorrhoeaBlennorrhoea
"A saga of HeLa cells". www.virology.ws. Information, Reed Business (9 June 1960). "New Scientist". Reed Business Information - ... "A Study by Fluorescence Microscopy of the Replication of Inclusion Blennorrhoea Virus in HeLa Cell Monolayers". Journal of ...
https://en.wikipedia.org/wiki/Blennorrhoea
Katarína HorákováKatarína Horáková
Jantovà, S.; Horàkovà, K. (1993). "9-Hydroxybenfluron induced inhibition of proliferation and metabolism in hela cells". Cell ... "Degeneration of HeLa-cells induced by the cytotoxic effect of isothiocyanates or by the starvation of cells". Acta Morphologica ... 2. Effect of l-alpha-ethyl ester of 2-isothiocyanate propionic acid on hela cell division and metabolism]. Biológia (in Slovak ... Horáková, K; Navarová, J; Nemec, P; Kettner, M (1974). "Effect of dactylarin of HeLa cells". The Journal of Antibiotics. 27 (6 ...
https://en.wikipedia.org/wiki/Katarína_Horáková
Dorothea Zucker-FranklinDorothea Zucker-Franklin
Zucker-Franklin, D.; Davidson, M.; Thomas, L. (1966). "The interaction of mycoplasmas with mammalian cells. I. HeLa cells, ... The use of electron microscopy to study blood cells would become a central theme of her research. Through this technique she ... Zucker-Franklin, D.; Grossi, C.E.; Greaves, M.F.; Marmont, A.M. (1981). Atlas of Blood Cells: Function and Pathology. Lea & ... That year she published the first edition of Atlas of Blood Cells: Function and Pathology in collaboration with Carlo Grossi; ...
https://en.wikipedia.org/wiki/Dorothea_Zucker-Franklin
MCM6MCM6
Holthoff HP, Baack M, Richter A, Ritzi M, Knippers R (1998). "Human protein MCM6 on HeLa cell chromatin". J. Biol. Chem. 273 ( ... Cell. Biol. 19 (12): 8003-15. doi:10.1128/MCB.19.12.8003. PMC 84885. PMID 10567526. Ishimi Y, Komamura-Kohno Y, You Z, Omori A ... Cell. Biol. 19 (12): 8003-15. doi:10.1128/MCB.19.12.8003. PMC 84885. PMID 10567526. Ishimi Y, Ichinose S, Omori A, Sato K, ... Ohtani K, Iwanaga R, Nakamura M, Ikeda M, Yabuta N, Tsuruga H, Nojima H (1999). "Cell growth-regulated expression of mammalian ...
https://en.wikipedia.org/wiki/MCM6
Murine respirovirusMurine respirovirus
HeLa cells can be infected with SeV; however, incubation of these cells with IFN-beta causes inhibition of SeV replication. ... Not all cancer cells have cell entry receptors for the virus and not all cancer cells express virus processing serine proteases ... involves the fusion of infected cells with adjacent target cells and remains an important mechanism of direct cell-to-cell ... "Directed cell fusion" below). The virus can fuse up to 50-100 cells adjacent to one primary infected cell. This multi-nuclear ...
https://en.wikipedia.org/wiki/Murine_respirovirus
AgrobacteriumAgrobacterium
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... By altering the hormone balance in the plant cell, the division of those cells cannot be controlled by the plant, and tumors ... The plasmid T-DNA is integrated semi-randomly into the genome of the host cell, and the tumor morphology genes on the T-DNA are ... This method transforms only cells in immediate contact with the bacteria, and results in transient expression of plasmid DNA. ...
https://en.wikipedia.org/wiki/Agrobacterium
Agrobacterium tumefaciensAgrobacterium tumefaciens
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... "Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis". Cell. 11 (2): ... This stimulates cell proliferation and gall formation. The T-DNA contains genes for encoding enzymes that cause the plant to ... The Ti plasmid integrates a segment of its DNA, known as T-DNA, into the chromosomal DNA of its host plant cells. A. ...
https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens
LIG4LIG4
Robins P, Lindahl T (September 1996). "DNA ligase IV from HeLa cell nuclei". The Journal of Biological Chemistry. 271 (39): ... This interaction stabilizes LIG4 protein in cells; cells that are deficient for XRCC4, such as XR-1 cells, have reduced levels ... variable degrees of immunodeficiency and reduced number of blood cells. Accumulation of DNA damage leading to stem cell ... The sensitivity of haematopoietic stem cells to lig4 deficiency suggests that lig4-mediated NHEJ is a key determinant of the ...
https://en.wikipedia.org/wiki/LIG4
Extrachromosomal circular DNAExtrachromosomal circular DNA
Smith CA, Vinograd J (August 1972). "Small polydisperse circular DNA of HeLa cells". Journal of Molecular Biology. 69 (2): 163- ... eccDNA are circular DNA that have been found in human, plant, and animal cells and are present in the cell nucleus in addition ... The researchers found tens of thousands of microDNAs in mouse tissues and cell lines, as well as human cell lines. In 2017, ... To test whether eccDNAs occur in non-cancer cells, mouse embryonic stem cells and Southern Blot analysis were used; the results ...
https://en.wikipedia.org/wiki/Extrachromosomal_circular_DNA
Origin of transferOrigin of transfer
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... At the start of bacterial conjugation, a donor cell will elaborate a pilus and signal to a nearby recipient cell to get in ... MGIs provide resistance to their host cells, and through bacterial conjugation, spread this advantage to other cells. With ... A 2017 study on MGIs revealed that they are able to integrate themselves into the genome of the receiving bacterial cells by ...
https://en.wikipedia.org/wiki/Origin_of_transfer
Ti plasmidTi plasmid
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... where plant cells of secondary tumours were found to lack any bacterial cells within. However, these tumour cells did possess ... Within the recipient cell, VirD2 will also work together with VirE2 to direct the transferred DNA to the recipient cell's ... These regions have features that allow the delivery of T-DNA into host plant cells, and can modify the host plant cell to cause ...
https://en.wikipedia.org/wiki/Ti_plasmid
Outline of cell biologyOutline of cell biology
Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (February 2001). "Genetic transformation of HeLa cells by ... Meristemic cell - Undifferentiated plants cells analogous to animal stem cells. Stem cell - Undifferentiated cells found in ... especially animal cells. Cell disruption, and cell unroofing - Methods for releasing molecules from cells. Cell fractionation ... Cell type - Distinct morphological or functional form of cell. When a cell switches state from one cell type to another, it ...
https://en.wikipedia.org/wiki/Outline_of_cell_biology
DDX46DDX46
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Articles with short description, ...
https://en.wikipedia.org/wiki/DDX46
SFRS6SFRS6
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Lemaire R, Winne A, Sarkissian M, Lafyatis R (March 1999). "SF2 and SRp55 regulation of CD45 exon 4 skipping during T cell ... Gui JF, Lane WS, Fu XD (June 1994). "A serine kinase regulates intracellular localization of splicing factors in the cell cycle ... Molecular Cell. 6 (2): 307-16. doi:10.1016/S1097-2765(00)00031-9. PMID 10983978. Fossey SC, Mychaleckyj JC, Pendleton JK, ...
https://en.wikipedia.org/wiki/SFRS6
NOL1NOL1
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 12, All ... Cell Biol. 4 (7): 529-33. doi:10.1038/ncb814. PMID 12080348. S2CID 24923289. Holsinger LJ, Ward K, Duffield B, Zachwieja J, ... Cell. 13 (11): 4100-9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849. Beausoleil SA, Jedrychowski M, Schwartz D, Elias ...
https://en.wikipedia.org/wiki/NOL1
SF3B1SF3B1
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Molecular Cell. 5 (5): 779-87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114. Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune ...
https://en.wikipedia.org/wiki/SF3B1
RBMXRBMX
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Cell. 154 (2): 452-64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131. "Infection and Immunity Immunophenotyping ( ... "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ...
https://en.wikipedia.org/wiki/RBMX
NFIANFIA
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Lu W, Quintero-Rivera F, Fan Y, Alkuraya ... Cell. 123 (5): 819-31. doi:10.1016/j.cell.2005.09.023. PMID 16325577. S2CID 6003724. Lin YL, Wang YH, Lee HJ (November 2006). " ...
https://en.wikipedia.org/wiki/NFIA
RBM7RBM7
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Rbm7 in epithelial cells plays a critical role in the development of fibrosis by regulating ncRNA decay and thereby the ... Molecular Cell. 74 (2): 254-267.e10. doi:10.1016/j.molcel.2019.01.033. PMC 6482433. PMID 30824372. Fukushima K, Satoh T, ... "Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the ...
https://en.wikipedia.org/wiki/RBM7
BAZ1BBAZ1B
August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of ... Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. BAZ1B+protein,+human at the US National ... Nature Cell Biology. 6 (12): 1236-44. doi:10.1038/ncb1196. PMID 15543136. S2CID 23842462. Andersen JS, Lam YW, Leung AK, Ong SE ...
https://en.wikipedia.org/wiki/BAZ1B
OSBPL11OSBPL11
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 3, All ...
https://en.wikipedia.org/wiki/OSBPL11
Cyclin T2Cyclin T2
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ... "Abrogation of signal-dependent activation of the cdk9/cyclin T2a complex in human RD rhabdomyosarcoma cells". Cell Death Differ ... Cell. 19 (4): 523-34. doi:10.1016/j.molcel.2005.06.027. PMID 16109376. Cottone G, Baldi A, Palescandolo E, Manente L, Penta R, ... Cell. Biol. 27 (13): 4641-51. doi:10.1128/MCB.00857-06. PMC 1951478. PMID 17452463. FactorBook CCNT2 v t e (Genes on human ...
https://en.wikipedia.org/wiki/Cyclin_T2
PALB2PALB2
August 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of ... This reduced fertility appears to be due to germ cell attrition resulting from a combination of unrepaired DNA breaks during ... June 2006). "Control of BRCA2 cellular and clinical functions by a nuclear partner, PALB2". Molecular Cell. 22 (6): 719-29. doi ... June 2006). "Control of BRCA2 cellular and clinical functions by a nuclear partner, PALB2". Molecular Cell. 22 (6): 719-29. doi ...
https://en.wikipedia.org/wiki/PALB2
STRN3STRN3
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. Bibcode: ... "A cell-cycle nuclear autoantigen containing WD-40 motifs expressed mainly in S and G2 phase cells". Biochem. Biophys. Res. ... Cell. Proteomics. 8 (1): 157-71. doi:10.1074/mcp.M800266-MCP200. PMC 2621004. PMID 18782753. Ewing RM, Chu P, Elisma F, Li H, ... Cell Res. 212 (2): 255-61. doi:10.1006/excr.1994.1141. PMID 7910562. Castets F, Rakitina T, Gaillard S, Moqrich A, Mattei MG, ...
https://en.wikipedia.org/wiki/STRN3
SNRPA1SNRPA1
Ohno M, Kataoka N, Shimura Y (December 1990). "A nuclear cap binding protein from HeLa cells". Nucleic Acids Research. 18 (23 ...
https://en.wikipedia.org/wiki/SNRPA1
MINK1MINK1
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Articles with short description, ...
https://en.wikipedia.org/wiki/MINK1
ChlorphenamineChlorphenamine
Arias-Montaño JA, Young JM (May 1993). "Characteristics of histamine H1 receptors on HeLa cells". European Journal of ... September 1994). "Stable expression of human H1-histamine-receptor cDNA in Chinese hamster ovary cells. Pharmacological ...
https://en.wikipedia.org/wiki/Chlorphenamine
SENP6SENP6
"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 6, All ... The Journal of Cell Biology. 174 (7): 939-49. doi:10.1083/jcb.200510103. PMC 2064386. PMID 17000875. Olsen JV, Blagoev B, Gnad ...
https://en.wikipedia.org/wiki/SENP6
Cervical cancerCervical cancer
In medical research, the most famous immortalized cell line, known as HeLa, was developed from cervical cancer cells of a woman ... 1951: First successful in-vitro cell line, HeLa, derived from biopsy of cervical cancer of Henrietta Lacks. 1976: Harald zur ... carcinoma Small cell carcinoma Neuroendocrine tumour Glassy cell carcinoma Villoglandular adenocarcinoma Though squamous cell ... It is due to the abnormal growth of cells that have the ability to invade or spread to other parts of the body. Early on, ...
https://en.wikipedia.org/wiki/Cervical_cancer
PANO1PANO1
When there is an abnormal expression of PANO1 in HeLa cells, scientists have seen a decrease in tumorigenicity in nude mice. ... PANO1 has been identified to modulate and stabilize p14ARF by stabilizing it and protecting it from degradation in HeLa cells. ... PANO1 is biased to being expressed in androgen sensitive cells compared to androgen insensitive cells. A predicted 3' UTR ... p14ARF is a protein that is a known tumor suppressor.It does this by controlling cell proliferation and cell survival, however ...
https://en.wikipedia.org/wiki/PANO1
MAPK8IP3MAPK8IP3
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... and c-Src are activated in human aortic smooth muscle cells by pressure stress". Mol. Cell. Biochem. 262 (1-2): 71-8. doi: ... 2006). "JSAP1/JIP3 cooperates with focal adhesion kinase to regulate c-Jun N-terminal kinase and cell migration". J. Biol. Chem ... 2001). "Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein". Cell. 103 (4): 583-94. doi:10.1016/ ...
https://en.wikipedia.org/wiki/MAPK8IP3
WIPI2WIPI2
... mRNA is readily detectable in several commonly used laboratory cell lines (HEK293A, HeLa, A431) and several cancer cell ... Curr Opin Cell Biol. 22 (2): 150-6. doi:10.1016/j.ceb.2009.11.013. PMID 20036114. * Polson HE, de Lartigue J, Rigden DJ, ... which is a lysosomal degradation pathway required for maintaining cell health, surviving periods of nutrient deprivation and ... lines, while WIPI1 expression is limited to cancer cells[citation needed] (but is also detected in many human tissues). The Atg ...
https://en.wikipedia.org/wiki/WIPI2
InterferonInterferon
... polymerase and protein kinase of interferon-treated HeLa cells". The Journal of Biological Chemistry. 254 (20): 10180-3. doi: ... A virus-infected cell releases viral particles that can infect nearby cells. However, the infected cell can protect neighboring ... and its expression is restricted to immune cells such as T cells and NK cells. All interferons share several common effects: ... Type II interferons are also released by cytotoxic T cells and type-1 T helper cells. However, they block the proliferation of ...
https://en.wikipedia.org/wiki/Interferon
Anterograde tracingAnterograde tracing
"Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells". Journal of Virology ... WGA enters the cell by binding to oligosaccharides, and is then taken up via endocytosis via a caveolae-dependent pathway. ... It was shown that pH and endocytosis are crucial for the HSV to infect a cell. Transport of the viral particles along the axon ... There is also a group of tracers that consist of protein products that can be taken up by the cell and transported across the ...
https://en.wikipedia.org/wiki/Anterograde_tracing
SFRS2IPSFRS2IP
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 12, All stub articles, Human ... Zhang WJ, Wu JY (Feb 1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing". Mol Cell Biol. 18 (2 ... 1999). "Antigens recognized by autologous antibody in patients with renal-cell carcinoma". Int. J. Cancer. 83 (4): 456-64. doi: ...
https://en.wikipedia.org/wiki/SFRS2IP
TACC3TACC3
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... The function of this gene has not yet been determined; however, it is speculated that it may be involved in cell growth and ... Expression of this gene is up-regulated in some cancer cell lines, and in embryonic day 15 in mice. GRCh38: Ensembl release 89 ... 2002). "The centrosomal protein TACC3 is essential for hematopoietic stem cell function and genetically interfaces with p53- ...
https://en.wikipedia.org/wiki/TACC3
Brucella suisBrucella suis
2-glucan mutants have reduced virulence in mice and are defective in intracellular replication in HeLa cells." Infectious ... capable of growing and reproducing inside of host cells, specifically phagocytic cells. They are also not spore-forming, ... B. suis, in return, has developed ways to counteract the host cell defense to survive in the macrophage and to deter host ... In addition, this furtive entry into macrophages does not affect the cell's normal trafficking. The smooth LPS also inhibits ...
https://en.wikipedia.org/wiki/Brucella_suis
Mitochondrial ferritinMitochondrial ferritin
2008). "The effects of frataxin silencing in HeLa cells are rescued by the expression of human mitochondrial ferritin" (PDF). ... Cell Biol. 40 (10): 2110-9. doi:10.1016/j.biocel.2008.02.010. hdl:10447/35800. PMID 18372207. Snyder AM, Wang X, Patton SM, et ... Cell. Biol. 26 (7): 2845-56. doi:10.1128/MCB.26.7.2845-2856.2006. PMC 1430308. PMID 16537925. Hasan MR, Tosha T, Theil EC (2008 ... J. Physiol., Cell Physiol. 293 (2): C641-9. doi:10.1152/ajpcell.00599.2006. PMID 17459943. S2CID 2673703. Campanella A, Rovelli ...
https://en.wikipedia.org/wiki/Mitochondrial_ferritin
YopE protein domainYopE protein domain
On the role of SycE in targeting YopE into HeLa cells". J. Biol. Chem. 274 (31): 22102-8. doi:10.1074/jbc.274.31.22102. PMID ... dendritic cells, and neutrophils. Evidence also suggests that CD8 T lymphocyte cells mediate protection against Yersinia by ... It not only infects the host cell but also protects the bacteria. It undergoes several mechanisms to evade the host's immune ... Additionally, it is thought that YopE targets the following immune cells, in particular: B lymphocytes, macrophages, ...
https://en.wikipedia.org/wiki/YopE_protein_domain
WDR44WDR44
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. v t e (Articles with short description, Short description matches Wikidata, ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
https://en.wikipedia.org/wiki/WDR44
CBLCCBLC
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... Cell. Biol. 22 (12): 4241-55. doi:10.1128/MCB.22.12.4241-4255.2002. PMC 133880. PMID 12024036. Szymkiewicz I, Kowanetz K, ... 2002). "C-Cbl is involved in Met signaling in B cells and mediates hepatocyte growth factor-induced receptor ubiquitination". J ... CBL proteins modulate downstream cell signaling (Keane et al., 1999).[supplied by OMIM] CBLC has been shown to interact with ...
https://en.wikipedia.org/wiki/CBLC
CPSF1CPSF1
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... Cell. Biol. 22 (1): 148-60. doi:10.1128/MCB.22.1.148-160.2002. PMC 134228. PMID 11739730. Maeda Y, Ito M, Harashima N, et al. ( ... Cell. Biol. 20 (5): 1515-25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326. PMID 10669729. de Vries H, Rüegsegger U, Hübner W ... Cell. 33 (3): 365-76. doi:10.1016/j.molcel.2008.12.028. PMC 2946185. PMID 19217410. Keller W, Bienroth S, Lang KM, Christofori ...
https://en.wikipedia.org/wiki/CPSF1
MYD88MYD88
"Ultrastructural distribution of the death-domain-containing MyD88 protein in HeLa cells". Experimental Cell Research. 243 (1): ... Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. ... connecting proteins that receive signals from outside the cell to the proteins that relay signals inside the cell. In innate ... since the immunological phenotype of human cells deficient in MYD88 is similar to cells from MyD88 deficient mice. However, ...
https://en.wikipedia.org/wiki/MYD88
DBR1DBR1
Arenas J, Hurwitz J (1987). "Purification of a RNA debranching activity from HeLa cells". J. Biol. Chem. 262 (9): 4274-9. doi: ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. v t e (Genes on human chromosome 3, All stub articles, Human ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. ...
https://en.wikipedia.org/wiki/DBR1
HSPA8HSPA8
"Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells". Cell Structure ... Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. Imai Y ... an apoptotic cell undergoes structural changes including cell shrinkage, plasma membrane blebbing, nuclear condensation, and ... Cell Biol. 36 (12): 2435-44. doi:10.1016/j.biocel.2004.02.013. PMID 15325583. Xie W, Zhang L, Jiao H, Guan L, Zha J, Li X, Wu M ...
https://en.wikipedia.org/wiki/HSPA8
Prostate cancerProstate cancer
HeLa cells). But where 5-LOX was in active state, zileuton could effectively inhibit progression, as in case of prostate cancer ... prostate cancer cell population harbors self-renewing long-term tumor-propagating cells that resist castration". Cell Stem Cell ... LNCaP cells express AR, but PC-3 and DU-145 cells express very little or no AR. The proliferation of LNCaP cells is androgen- ... Prostate cancer cells are generally devoid of zinc. Prostate cancer cells save energy by not making citrate, and use the ...
https://en.wikipedia.org/wiki/Prostate_cancer
ELF2ELF2
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... Cell. Biol. 16 (9): 5091-106. doi:10.1128/MCB.16.9.5091. PMC 231510. PMID 8756667. "Entrez Gene: ELF2 E74-like factor 2 (ets ... Cell. Biol. 19 (5): 3635-44. doi:10.1128/mcb.19.5.3635. PMC 84165. PMID 10207087. Christensen RA, Fujikawa K, Madore R, et al ... 1997). "Elf-2, a rhombotin-2 binding ets transcription factor: discovery and potential role in T cell leukemia". Leukemia. 11 ( ...
https://en.wikipedia.org/wiki/ELF2
Index of biochemistry articlesIndex of biochemistry articles
Hela cell - helminth protein - helper T cell - hemopexin - hemoglobin - herpes simplex virus protein vmw65 - heterocyclic ... cell - cell adhesion molecule - cell biology - cell cycle protein - cell membrane - cell membrane transport - cell nucleus - ... systems biology T cell - T-cell antigen receptors - tachykinin - tachykinin receptor - talin protein - tandem repeat sequence ... memory B cell - memory T cell - Mendelian inheritance - metabolic pathway - metabolism - metabotropic glutamate receptor - ...
https://en.wikipedia.org/wiki/Index_of_biochemistry_articles
Glauco Tocchini-ValentiniGlauco Tocchini-Valentini
Perkins, K. K., Furneaux, H. and Hurwitz, J., "Isolation and characterization of an RNA ligase from HeLa cells," Proc. Natl. ... and kidney cells; the characteristics of these amphibian cells and their enzymes were related to mammalian cells and their ... He currently resides in Rome, Italy as director at the Institute of Cell Biology. He is also the coordinator for European Mouse ... Much of his work was characterized using various Xenopus laevis cell types, including oocytes, unfertilized eggs, ...
https://en.wikipedia.org/wiki/Glauco_Tocchini-Valentini
ANKRD17ANKRD17
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... 2002). "Hepatic differentiation of murine embryonic stem cells". Exp. Cell Res. 272 (1): 15-22. doi:10.1006/excr.2001.5396. ... Cell Biol. 6 (2): 97-105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986. Beausoleil SA, Jedrychowski M, Schwartz D, et al ...
https://en.wikipedia.org/wiki/ANKRD17
Howard W. JonesHoward W. Jones
The cells, later known as HeLa cells, grew at an astonishing rate in the lab and were shipped and sold to researchers for ... Research with the cells helped to facilitate medical breakthroughs, including the vaccines for polio and human papillomavirus, ... though controversy later arose because the cells were being used without the knowledge of Lacks or her family. Jones's role in ...
https://en.wikipedia.org/wiki/Howard_W._Jones
Human HeLa cancer cells, cytokinesis | Wellcome CollectionHuman HeLa cancer cells, cytokinesis | Wellcome Collection
... cell division). The chromosomes are red (DAPI stain for DNA). The microtubules of the cytoskeleton/spindle are purple, and the ... A deconvolved wide-field fluorescence microscope image of human HeLa cancer cells undergoing cytokinesis ( ... A deconvolved wide-field fluorescence microscope image of human HeLa cancer cells undergoing cytokinesis (cell division). The ... Human HeLa cancer cells, cytokinesis. .. Paul Andrews/Univ. Dundee.. Attribution 4.0 International (CC BY 4.0) ...
https://wellcomecollection.org/works/e22q6dmh
HeLa tissue culture cells | 1996 Photomicrography Competition | Nikons Small WorldHeLa tissue culture cells | 1996 Photomicrography Competition | Nikon's Small World
HeLa tissue culture cells. Dr. Kuruganti G. Murti Affiliation. St. Jude Childrens Research Hospital. Memphis, Tennessee, USA. ...
https://www.nikonsmallworld.com/galleries/1996-photomicrography-competition/hela-tissue-culture-cells
Pre-made Human GBA knockout HeLa cell lysate (ab256929) | AbcamPre-made Human GBA knockout HeLa cell lysate (ab256929) | Abcam
GBA KO cell lysate available now. KO validated by Western Blot (WB). Free of charge wild type control available. Knockout ... Lane 1: Wild-type HeLa cell lysate (20µg). Lane 2: GBA knockout HeLa cell lysate (20µg). Lanes 1- 2: Merged signal (red and ... Lane 1: Wild-type HeLa cell lysate (20µg). Lane 2: GBA knockout HeLa cell lysate (20µg). Lanes 1- 2: Merged signal (red and ... Cell lines and Lysates. Multiplex miRNA assays. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. ...
https://www.abcam.com/human-gba-knockout-hela-cell-lysate-ab256929.html
MBS3248705 | Hela Cell Lysate | MyBiosourceMBS3248705 | Hela Cell Lysate | MyBiosource
Hela Cell Lysate. Cat# MBS3248705. Supplier: MyBiosource. Europe Distribution. ... HeLa Cell Lysate (Whole Cell) , MBS1750956 , MybiosourceProduct Short Name: [HeLa Cell]Product Name Synonyme: N/AOther Names: N ... Hela Whole Cell Lysate , MBS435034 , MyBiosourceProduct Short Name: [Hela Whole Cell Lysate]Product Name Synonyme: N/AOther ... HeLa Cell Lysate, Heat Shocked , MBS657048 , MyBiosourceProduct Short Name: [HeLa Cell Lysate, Heat Shocked]Product Name ...
https://biocheminfo.org/mbs3248705-hela-cell-lysate/
Estradiol-induced mitotic delay in HeLa cells: reversal by calcium chloride and putrescine. | Semantic ScholarEstradiol-induced mitotic delay in HeLa cells: reversal by calcium chloride and putrescine. | Semantic Scholar
Estradiol-induced mitotic delay in HeLa cells: reversal by calcium chloride and putrescine. by P. Rao ... Division delay in HeLa cells in Chinese hamster cells. A time-lapse study.. *G. Froese ... Structural specificity of estrogens in the induction of mitotic chromatid non-disjunction in HeLa cells.. *P. Rao, J. Engelberg ... HeLa Cells: Effects of Temperature on the Life Cycle. *P. Rao, J. Engelberg ...
https://www.semanticscholar.org/paper/Estradiol-induced-mitotic-delay-in-HeLa-cells%3A-by-Rao/b55dea00b04122784cea124d84e1a30a26b6649f
AID 597373 - Cytotoxicity against human HeLa cells at 12.5 to 100 uM after 2 hrs - PubChemAID 597373 - Cytotoxicity against human HeLa cells at 12.5 to 100 uM after 2 hrs - PubChem
Cytotoxicity against human HeLa cells at 12.5 to 100 uM after 2 hrs. ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/597373
Have you ever heard of research cells called HeLa? - Cancer Survivors NetworkHave you ever heard of research cells called HeLa? - Cancer Survivors Network
I understand that HeLa cells have actually contaminated other cell cultures in labs because cells can float through the air and ... Her cells have even been sent into space! Google HeLa cells for more information. There ought to be a statue erected in her ... Ha, Moli, maybe we can all get rich selling our cells! Instead of HeLa, mine would be called CaVi. Kind of has a nice ring to ... So many of HeLa cells have been reproduced -the designation comes from the first two letters of her first and last names- that ...
https://csn.cancer.org/discussion/comment/1512286/
HeLa cell volume - Human Homo sapiens - BNID 103719HeLa cell volume - Human Homo sapiens - BNID 103719
Correlation between cell enlargement and nucleic acid and protein content of HeLa cells in unbalanced growth produced by ... Abstract: HeLa cells in monolayer cultures were treated with the following inhibitors of DNA synthesis: mitomycin C, nitrogen ... Please note-BNID 103725 gives HeLa cell volume range of 1,200-4,290µm^3. See BNID 105879. ... Cell number was obtained with a Coulter counter, while cell sizing was performed with the automatic particle size distribution ...
https://bionumbers.hms.harvard.edu/bionumber.aspx?s=n&v=20&id=103719
Functional Characterization of an Organic Cation Transporter (hOCT1) in a Transiently Transfected Human Cell Line (HeLa) |...Functional Characterization of an Organic Cation Transporter (hOCT1) in a Transiently Transfected Human Cell Line (HeLa) |...
HeLa cell culture and transfection.. HeLa cells were obtained from the UCSF Cell Culture Facility. Original stocks were from ... of the given compounds in pTargeT-hOCT1-transfected HeLa cells. Controls represent uptake of 14C-TEA in the HeLa cells ... Transient expression of hOCT1 in HeLa cells over time. After transfection of pTargeT-hOCT1 (•) and empty vector (○) in HeLa ... The cells were maintained in Nunc cell culture flasks (Nalge Nunc International, Naperville, IL). The cells were seeded at a ...
https://jpet.aspetjournals.org/content/286/1/354?ijkey=49edd0e9734a143255236750c98562c21bf7ffa6&keytype2=tf_ipsecsha
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: | Thermo Fisher Scientific - USImaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: | Thermo Fisher Scientific - US
Knock-down of NDRG2 sensitizes cervical cancer Hela cells to cisplatin through suppressing Bcl-2 expression | BMC Cancer | Full...Knock-down of NDRG2 sensitizes cervical cancer Hela cells to cisplatin through suppressing Bcl-2 expression | BMC Cancer | Full...
... cell differentiation and tumor suppression. We have found that NDRG2 expression in cervical cancer Hela cells increases ... However, Bcl-2 mRNA level was not changed in Hela cells with down-regulation of NDRG2. Further study indicated that miR-15b and ... In the present study, RNA interference was employed to down-regulate NDRG2 expression in Hela cells. RT-PCR and Western blot ... In vitro drug sensitivity assay revealed that suppression of NDRG2 could sensitize Hela cells to cisplatin. Down-regulation of ...
https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-12-370/figures/3
Dr. George Gey: The Researcher Who Immortalized HeLa Cells | Shortform BooksDr. George Gey: The Researcher Who Immortalized HeLa Cells | Shortform Books
He used her tissue to make the immortal HeLa cells. Learn more. ... HeLa cells were energizing cell research. Dr. George Gey had ... That company would become the first for-profit cell distribution firm, shipping out HeLa cells for less than 50 dollars a vial ... He patented neither the HeLa cell line nor the roller drum he created to culture cells-either of which would have made him a ... in order for the cells to replicate. With the roller drum created by George Gey, HeLa cells could be grown was possible. ...
https://www.shortform.com/blog/dr-george-gey-hela-cells/
EMF-Portal | White light-electromagnetic field interaction causes a localized destruction of cultured HeLa cellsEMF-Portal | White light-electromagnetic field interaction causes a localized destruction of cultured HeLa cells
White light-electromagnetic field interaction causes a localized destruction of cultured HeLa cells med./bio. By: Alvarez- ...
https://www.emf-portal.org/en/article/879
The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the...The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the...
The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the ... The available evidence indicates that the levels of mtRNase P detected in HeLa cells should be fully adequate to satisfy the ... The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the ... The mitochondrion-associated RNase P activity (mtRNase P) was extensively purified from HeLa cells and shown to reside in ...
https://scholars.duke.edu/display/pub794250
HeLa cells - A Little Bit of All of It
div align="center">,a href="http://naturallifemom.com/category/natural-living-series/" title="Natural Living Series at A Little Bit of All of It",,img src="http://naturallifemom.com/wp-content/uploads/2013/04/NLS250x250.jpg.jpg" alt="Natural Living Series at A Little Bit of All of It" style="border:none;" /,,/a,,/div ...
https://naturallifemom.com/tag/hela-cells/
Weekly stem cell reads: parabiosis, UC Davis trial, HeLa lawsuit, gray hair - The NicheWeekly stem cell reads: parabiosis, UC Davis trial, HeLa lawsuit, gray hair - The Niche
Professor Knoepfler notes several milestones recently here for the stem cell blog and discusses parabiosis. ... Heterochronic parabiosis induces stem cell revitalization and systemic rejuvenation across aged tissues, Cell Stem Cell. Were ... Weekly stem cell reads: parabiosis, UC Davis trial, HeLa lawsuit, gray hair. By Professor Paul Knoepfler, Ph.D. / 3 Comments / ... May 29, 2022 May 28, 2022 / parabiosis, stem cell blog, Stem Cells / Parabiosis, Stem cell, Young blood ...
https://ipscell.com/2022/05/weekly-stem-cell-reads-parabiosis-uc-davis-trial-hela-lawsuit-gray-hair/
Retinoicacid-inducible gene-I is induced by interferon-γ and regulates CXCL11expression in HeLa cells | CiNii Research
Retinoicacid-inducible gene-I is induced by interferon-γ and regulates CXCL11expression in HeLa cells. Author Yuzawa E, ... Retinoicacid-inducible gene-I is induced by interferon-γ and regulates CXCL11expression in HeLa cells ...
https://cir.nii.ac.jp/crid/1010000782438540548
Evidence for RNA linked to nascent DNA in HeLa cells. | Journal of Cell Biology | Rockefeller University PressEvidence for RNA linked to nascent DNA in HeLa cells. | Journal of Cell Biology | Rockefeller University Press
DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled ... D D Olgiati, B G Pogo, S Dales; Evidence for RNA linked to nascent DNA in HeLa cells.. J Cell Biol 1 March 1976; 68 (3): 557- ... Evidence for RNA linked to nascent DNA in HeLa cells. D D Olgiati, D D Olgiati ... Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further ...
https://rupress.org/jcb/article/68/3/557/18653/Evidence-for-RNA-linked-to-nascent-DNA-in-HeLa
Nutritional and metabolic requirements for the infection of HeLa cells by Salmonella enterica serovar Typhimurium. - Oxford...
Typhimurium in HeLa cells. We also deleted genes encoding enzymes involved in the utilization of gluconeogenic substrates and ... Typhimurium utilizes glycolysis for replication within HeLa cells; however, glycolysis was not absolutely essential for ... borne pathogen and infects via ingestion followed by invasion of intestinal epithelial cells and phagocytic cells. In this ... HeLa). We deleted the key glycolytic genes, pfkA and pfkB to show that S. ...
https://www.neuroscience.ox.ac.uk/publications/466305?d355c2b2-753e-11ed-a565-061ab7f93640
Automatic Time-lapse Cell Membrane Imaging: C2C12, Hela cellAutomatic Time-lapse Cell Membrane Imaging: C2C12, Hela cell
Cell Membrane of Live Hela Conditions:. Cell line: Hela cell, Scan size: 30 μm x 15 μm. Glass pipette, ARS mode, Medium: 1x PBS ... Automatic Time-lapse Cell Membrane Imaging: C2C12, Hela cell Brian Choi, Bio-application scientist. For more information, ... In this study, the cell membrane morphology of living C2C12 (myoblast) and HeLa cells, as well as the dynamics of those ... The continuous imaging of cell membranes unveils the detail morphological physiology of C2C12 and HeLa cells by showing outward ...
https://www.parksystems.com/learning-center/nano-academy/analyze-cells/analyze-cells-sicm/523-automatic-time-lapse-cell-membrane-imaging
HeLa cells
A series of oil paintings that explore the emerging identity of the immortal cell line, HeLa. ... HeLa cells searching no. 2 Two panels 30 x 10 in, one panel 30 x 30 in Oil on canvas 2012 ... HeLa cells searching no. 22 Two panels, each 20 x 10 in Oil on wood 2012 Sold. ... HeLa cells searching no. 4 Each panel 36 x 36 in Oil on canavs 2012 ...
https://www.joanaricou.com/hela
AmBisome inhibits the adherence of Candida albicans to HeLa cells by Krystyna Konopka, Barbara Dorocka-Bobkowska et al."AmBisome inhibits the adherence of Candida albicans to HeLa cells" by Krystyna Konopka, Barbara Dorocka-Bobkowska et al.
Candidal adherence to epithelial cells is significantly reduced when antifungal polyenes are present during the ... albicans to HeLa cells. Methods: The adherence of C. albicans to HeLa cells was determined as described by Samaranayake et al ... The cytotoxic effect of the drugs on HeLa cells was determined by an Alamar Blue assay. Results: AmBisome was not toxic in the ... Konopka, K., Dorocka-Bobkowska, B., & Düzgüneş, N. (2004). AmBisome inhibits the adherence of Candida albicans to HeLa cells. ...
https://scholarlycommons.pacific.edu/dugoni-facpres/348/
HeLa Cells - Trend FoolHeLa Cells - Trend Fool
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in Wuhan, China, in late December 2019, and subsequently led to the coronavirus disease 2019... ...
https://trendfool.com/topic/hela-cells/?order=most_viewed
HeLa cellsHeLa cells
Tag Archives: HeLa cells February 21, 2011 · 12:42 pm Simple Twist of Fate. The Immortal Life of Henrietta Lacks by Rebecca ... They are the so-called HeLa cells that are used by researchers throughout the world to advance the knowledge of how to fight ... They are the so-called HeLa cells that are used by researchers throughout the world to advance the knowledge of how to fight ... They have not received any money, and although HeLa cells are sold for medical research, the family does not have health care. ...
https://josephsreviews.wordpress.com/tag/hela-cells/
Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal...Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal...
... cells in vitro and their subsequent transplantation into the subretinal space is expected to improve the outcome of cell ... iRPE cells exhibited RPE specific properties, including phagocytic ability, epithelial polarity, and gene expression profile. ... In addition, high expression of PTPN13 in iRPE cells endows them with an epithelial-to-mesenchymal transition (EMT)-resistant ... After grafting into the subretinal space of the sodium iodate-induced rat AMD model, iRPE cells demonstrated a better ...
https://www.nature.com/articles/s41419-022-05199-5?error=cookies_not_supported&code=b11dc825-6928-452c-80f4-a99b44f581cb
HeLa cells | Incredible Panic RulesHeLa cells | Incredible Panic Rules
... for the cell line named after her unusual, fast-growing cells. Over the years, HeLa cells have been used for cancer research, ... Tag Archives: HeLa cells Henrietta Lacks grandkids: "How can you judge the 1950s by the ethics of today?" Leave a reply ... "I first heard about the HeLa cells when I was 16, at community college taking a class for high school credit," said Skloot. "My ... During the years that HeLa had become increasingly renowned-the first cells to go into space, the first whose genes were mapped ...
https://chrislombardi.me/tag/hela-cells/
HeLa Cells - Asylum Research Learning CentreHeLa Cells - Asylum Research Learning Centre
HeLa Cells. TIRF data overlaid on rendered AFM topography of HeLa Cells, 40 ?m scan. Image courtesy of M. Kellermayer, ...
https://afm.oxinst.com/learning/view/image/hela-cells
ZEISS Axio Vert A1 Bio inverted microscopeZEISS Axio Vert A1 Bio inverted microscope
With DIC you visualize even the finest structures in your cells. And the new IVF contrast system is particularly impressive in ... You are working with fluorescence-labeled cells or specify transfection rates? With Axio Vert.A1 your samples remain safe in ... You examine embryos and assess cell nuclei iHMC shows nucleus shape and nucleoli brilliantly. Structures such as the Zona ... The Growth of Cells in Culture Media Outside the Organism in an Artificial Environment ...
https://www.zeiss.com/microscopy/en/products/light-microscopes/widefield-microscopes/axio-vert-a1-for-biology.html
HeLa cells: A privacy dilemma - Porterhouse MedicalHeLa cells: A privacy dilemma - Porterhouse Medical
... including how her cells influenced medical research and the controversy surrounding the unconsented use of her cells. ... Use of HeLa cells in medical research. HeLa cells have become one of the most frequently used cell lines, holding a pivotal ... which resulted in the formation of the first human cell line [2,4]. These infamous cells, coined HeLa cells after the first ... Henrietta Lacks, HeLa cells, and cell culture contamination. Arch Pathol Lab Med 2009; 133 (9): 1463-1467. ...
https://www.porterhousemedical.com/news/hela-cells-a-privacy-dilemma/
Henrietta LacksEpithelial cells1951Growth of HeLa cellsLive HeLa cellsWhole Cell LysateVitroApoptosisProliferationLysateCytotoxicityHenrietta'sProteinLineAssayHuman cervical cancer cellMitochondriaAntibodyGenomeAbstractTransfectionPlasmidAdherenceChromosomesTumor cellsMorphologyCulturesGeorge GeyMonolayerTissue cultureChromatinControl siRNAMembraneSignificantlyStrainGenesLacksBacterialLysatesMammalianHumansCompoundsCytoplasmLinesBiomedical2020FibroblastsFluorescenceApoptotic0.05StableCancer cellsOrganismInfectionDifferentiationExposureMutationToxicityDonor
Henrietta Lacks20
  • Who was the doctor that turned the tissue from Henrietta Lacks into the HeLa cells? (shortform.com)
  • Court to consider whether Henrietta Lacks' family's 'unprecedented' lawsuit over use of her cells can continue , Baltimore Sun. We should all recall that the cell lines we use in the lab often come from real people. (ipscell.com)
  • Henrietta Lacks died in 1951 of cervical cancer but her cells are still alive. (wordpress.com)
  • The descendents of Henrietta Lacks have never benefited from the use of her cells, leaving aside the issue of whether they were properly appropriated. (wordpress.com)
  • At the Kimmel Cancer Center (part of Thomas Jefferson University), the crowd was made up of scientists and physicians, many of whom knew Henrietta Lacks only as HeLa, for the cell line named after her unusual, fast-growing cells. (chrislombardi.me)
  • My teacher said what all teachers said in those days: 'There are these cells, there was this woman, her name was Henrietta Lacks, and she was black. (chrislombardi.me)
  • HeLa cells, now ubiquitous in medical research, originated from an aggressive cervical cancer that killed Henrietta Lacks, who was being treated at the Johns Hopkins Hospital in Baltimore. (medscape.com)
  • The story of how HeLa cells came to be used in medical research was recounted most recently in The Immortal Life of Henrietta Lacks by Rebecca Skloot, which was published in 2010. (medscape.com)
  • This article explores the extraordinary story of Henrietta Lacks, including how her cells influenced medical research and the controversy surrounding the unconsented use of her cells. (porterhousemedical.com)
  • The cancerous cells obtained from Henrietta Lacks without her permission were later used for research by Dr George Otto Gey, who was, at the time, Director of the Tissue Culture Laboratory at the Johns Hopkins Hospital. (porterhousemedical.com)
  • These infamous cells, coined 'HeLa' cells after the first and last names of Henrietta Lacks, have now been used countless times over the years, contributing towards a wide array of scientific discoveries [4]. (porterhousemedical.com)
  • The HeLa cell line was established in 1951 from cervical cancer cells taken from a patient, Henrietta Lacks. (elsevier.com)
  • Pratt's research inquiry essay focuses on the medical ethics issues involved in the case of Henrietta Lacks and her cancer cells (known as HeLa,) which have been used and reused in medical research without hers or her family's consent. (cortland.edu)
  • The HeLa cell line was the first immortal human cell line that George Otto Gey, Margaret Gey, and Mary Kucibek first isolated from Henrietta Lacks and developed at The Johns Hopkins Hospital in Baltimore, Maryland, in 1951. (asu.edu)
  • Henrietta Lacks, HeLa cells, and cell culture contamination. (wikidata.org)
  • However, the discovery of these cells, by then known as the HeLa cells (named for the first letters of Henrietta Lacks' name), had given rise to a multimillion-dollar industry. (inthewaitingroom.org)
  • According to the Associated Press , Johns Hopkins University and the family of Henrietta Lacks announced a new building on the school's campus in East Baltimore will be named after the woman whose cells were taken without her consent and widely used in revolutionary cell research. (blackthen.com)
  • Last year, the city of Baltimore designated October 4 as Henrietta Lacks Day to recognize the contributions of the woman behind the HeLa cells. (blackthen.com)
  • Her name was Henrietta Lacks, but scientists know her as HeLa. (who.int)
  • To evaluate the ability of A3 enzymes in editing the genomes of MPXV, HeLa (Henrietta Lacks, human epithelial cell line) cells were transfected with the six aforementioned cytidine deaminases, followed by the 2022 MPXV strain infection. (socialworker-findoffice.com)
Epithelial cells6
  • SummaryThe proliferation of ER+ malignant mammary epithelial cells (MMEC), MCF-7 and T-47D, was markedly inhibited by 10µM 17β-estradiol (E2), while the ER− line, MDA-MB-231, was minimally affected. (semanticscholar.org)
  • It is a food--and water--borne pathogen and infects via ingestion followed by invasion of intestinal epithelial cells and phagocytic cells. (ox.ac.uk)
  • Objectives: Candidal adherence to epithelial cells is significantly reduced when antifungal polyenes are present during the "adherence phase", but the treatment does not result in detachment of cell-associated yeasts. (pacific.edu)
  • It is characterized by visual function damage caused by apoptosis and loss of function of retinal pigment epithelial cells (RPE) and retinal photoreceptor cells [ 2 ]. (nature.com)
  • The human body constantly sheds cheek, or squamous epithelial, cells. (microscopyu.com)
  • Materials and Methods Cell Culture and RNA Extraction Two subclones of W12 cervical epithelial cells with HPV16 in differing physical states were a gift from Dr. Paul Lambert (University of Wisconsin, Madison, WI). (irjs.info)
19513
  • In 1951, the cancer cells were removed and used without her knowledge or consent, a practice that was not unusual at that time. (medscape.com)
  • In its press release, the GSA notes that, since their isolation in 1951, HeLa cells have been the most widely used human cell line in research, and have enabled momentous scientific breakthroughs, including the development of the polio vaccine, the understanding of the role telomerase plays in aging, and research that established human papillomavirus as a causative agent in many types of cervical cancer. (medscape.com)
  • Lacks died of cervical cancer in 1951 at the university where researchers soon discovered her cells reproduced indefinitely in test tubes. (blackthen.com)
Growth of HeLa cells2
  • Apoptotic effect of iron-free bovine lactoferrin inhibited the growth of HeLa cells after 48 hours of treatment while the diferric-bLf was ineffective in the concentration range tested (from 1 to 12.5 μM). (univaq.it)
  • Classes were generated using rank-sum technique and the top five hits (Olmesartan, Verteporfin, Saprisartan, Atorvastatin and Lapatinib) were tested experimentally to determine the inhibitory effects on growth of HeLa cells in vitro . (medicinelakex1.com)
Live HeLa cells1
  • A cell based/cell context system has been designed to allow researchers to examine and interrogate the HR process in live HeLa cells. (topogen.com)
Whole Cell Lysate1
  • MyBiosourceProduct Short Name: [Hela Whole Cell Lysate]Product Name Synonyme: N/AOther Names: N/AProduct Gene Name: N/AProduct Gene Name Synonyme: N/AOther Gene. (biocheminfo.org)
Vitro8
  • Mitotic inhibition and aneuploidy induction by naturally occurring and synthetic estrogens in Chinese hamster cells in vitro. (semanticscholar.org)
  • Effects of pharmacological concentrations of estrogens on proliferation and cell cycle kinetics of human breast cancer cell lines in vitro. (semanticscholar.org)
  • The cell cycle changes associated with estrogen-induced growth inhibition in vitro are identical to those observed during regression of ER positive but not ER negative human tumor xenografts in nude mice. (semanticscholar.org)
  • Therefore, the differentiation of MSCs into retinal pigment epithelial (RPE) cells in vitro and their subsequent transplantation into the subretinal space is expected to improve the outcome of cell therapy. (nature.com)
  • This was the first successful attempt to immortalize human-derived cells in vitro. (elsevier.com)
  • In vitro response of cervical cancer cell lines CaSki, HeLa, and ME-180 to the antiestrogen tamoxifen. (wikidata.org)
  • HeLa cells are the backbone of modern medicine, being cited in over 110,000 scientific articles and giving rise to major medical treatments, such as the polio vaccine, cancer treatments, in vitro fertilization, and more. (inthewaitingroom.org)
  • In addition, the in vitro growth inhibitory activity of these compounds against human cancer cell lines Hela, A549, HepG-2 and MCF-7, were also tested. (chinaxiv.org)
Apoptosis9
  • Down-regulation of NDRG2 enhanced cisplatin-induced apoptosis of Hela cells. (biomedcentral.com)
  • On the whole, apo-bLf triggered apoptosis of HeLa cells upon oxygen radicals burst and GSH decrease. (univaq.it)
  • We investigated the role ofMeV-P protein of Edmonston vaccine strain as modulator of apoptosis in cervical cancer cell line (HeLa) and found thatMeV-P protein is anti-apoptotic and enhances cell proliferation. (who.int)
  • Lira & F. Chiang) Induces Apoptosis in the Human Cervical Cancer HeLa Cell Line. (bvsalud.org)
  • Fruit Extract of Sechium chinantlense (Lira & F. Chiang) Induces Apoptosis in the Human Cervical Cancer HeLa Cell Line. (bvsalud.org)
  • Additionally, SCH arrested cell cycle progression but only in HeLa cells and induced apoptosis , as shown by phosphatidylserine translocation and caspase-3 activation, while Cis did so in both cell lines . (bvsalud.org)
  • Exploration of the mechanism of action of SCH in HeLa cells suggests that apoptosis was mediated by the intrinsic signaling pathway since there was no activation of caspase-8 , but there was a release of cytochrome -c. (bvsalud.org)
  • These findings suggest that the SCH extract has the potential to selectively kill tumor cells by promoting apoptosis , without harming nontumor cells . (bvsalud.org)
  • Apoptosis is the most well known processes by compounds were prepared as 3mg/ml concentration which cell death is mediated by at least 14 members of stock solutions in dimethyl sulfoxide (DMSO). (medicinelakex1.com)
Proliferation7
  • The represented image shows cell proliferation and growth of clusters after 21 days in incubation. (ismicroscopy.org.il)
  • Sechium edule ( Cucurbitaceae ) is a commercial species of chayote and is just one of several species in the genus Sechium, whose extracts inhibit proliferation in tumor cell lines . (bvsalud.org)
  • In the present study, HeLa cervical cancer and HaCaT normal cell lines were treated with SCH and cell proliferation was inhibited in both cell lines in a dose -dependent manner similar to the effect of the antineoplastic agent cisplatin (Cis). (bvsalud.org)
  • It was observed that proliferation of HeLa cells could be significantly inhibited by Atorvastatin in a concentration dependent manner. (medicinelakex1.com)
  • Wound healing is an extremely complex process consists of hemostasis, inflammation, cell proliferation and scar tissue remodeling phases. (fabad.org.tr)
  • Bioinks, bioprinting parameters, and post-bioprinting processing all impact viability and functionality of cells, which in turn affect subsequent cellular events, such as proliferation, differentiation, and tissue formation. (sigmaaldrich.com)
  • The bioink should not only provide structural, physical, and mechanical support to the embedded cells, but also supply them with the essential biological and chemical cues for cell survival, proliferation, and differentiation required for tissue morphogenesis and homeostasis. (sigmaaldrich.com)
Lysate3
  • Knockout cell lysate achieved by CRISPR/Cas9. (abcam.com)
  • Heat reconstituted cell/tissue lysate at 100C for 10min, cool down and load on to gel. (biocheminfo.org)
  • WB: THP-1, HAP1, PANC-1 and HeLa cell lysate. (abcam.com)
Cytotoxicity7
  • The compound was isolated and evaluated for their molecular docking studies using BIO-VIA Discovery studio and cytotoxicity activity towards human cervical cancer cell line (HeLa) by using MTT colorimetric assay techniques. (derpharmachemica.com)
  • Exposure of HeLa cells to 6.0 uM potassium dichromate (Cr) resulted in cytotoxicity, cell death and a significant reduction in the cellular level of eukaryotic translation initiation factor 4E - the rate-limiting factor required for mRNA translation. (cdc.gov)
  • Specific silencing of the eIF4E gene's expression with a small interfering RNA (siRNA) also resulted in significant cytotoxicity and cell death. (cdc.gov)
  • Furthermore, the eIF4E silenced cells were significantly more susceptible to the Cr-induced cytotoxicity compared with the control cells suggesting that the Cr-induced toxicity in HeLa cells was, at least in part, due to the decreased cellular level of eIF4E protein. (cdc.gov)
  • In summary, our results demonstrate that the Cr-induced ubiquitination and degradation of the phosphorylated eIF4E protein in HeLa cells resulted in a decreased cellular level of cyclin D1 protein leading to cytotoxicity and cell death. (cdc.gov)
  • A luminescence cell viability assay (ATPLite®, Waltham, MA) to determine cytotoxicity was used. (cdc.gov)
  • Curves consisting of compound and cells only were compared to cell only wells to determine cytotoxicity. (cdc.gov)
Henrietta's5
  • Henrietta's cells were incredibly prolific, so much so that now trillions of her cells have been growing in labs all over the world and deviding now for 64 years! (cancer.org)
  • In a cruel twist, as Henrietta's health declined, HeLa cells were energizing cell research . (shortform.com)
  • That's because the case is made that Henrietta's cells were, in effect, stolen from her by Johns Hopkins Hospital. (wordpress.com)
  • So he collected them, but until he got Henrietta's, the cells just always died. (chrislombardi.me)
  • It was not until 2013 that the Lacks family were given any authority over the use of the HeLa cell line - over half a century after Henrietta's cells were first used [2]. (porterhousemedical.com)
Protein14
  • If you require a native form of the protein please use the live cell version - found here . (abcam.com)
  • Correlation between cell enlargement and nucleic acid and protein content of HeLa cells in unbalanced growth produced by inhibitors of DNA synthesis. (harvard.edu)
  • The concentration of each inhibitor used was, in most cases, just sufficient to arrest cell multiplication and all produced unbalanced growth in the sense that the synthesis of RNA and protein were only partially inhibited while DNA synthesis stopped. (harvard.edu)
  • These active compounds were also effective in colon carcinoma Caco-2 cells, and some of them increased the expression of p62 protein, a typical substrate of autophagic proteolysis, indicating that phytochemicals impact p62 levels in autophagy-dependent and/or -independent manners. (tokushima-u.ac.jp)
  • MeV-P protein recruits the largeprotein (L), i.e. viral RNA-depended RNA polymerase (RdRp), to ensure viral replication in host cell. (who.int)
  • Steady-state protein levels of the TFIIA tau, alphabeta, and gamma subunits were significantly reduced when human embryonal (ec) and hepatic carcinoma cell lines were stimulated to differentiate with either all-trans-retinoic acid (ATRA) or sodium butyrate. (nih.gov)
  • The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). (cellsignal.com)
  • The Cr-induced reduction in the eIF4E protein level in HeLa cells was independent of the gene's transcription. (cdc.gov)
  • Pre-exposure of the cells to ALLN and MG132 - inhibitors of proteasome activity, blocked the Cr-induced degradation of eIF4E protein. (cdc.gov)
  • Pretreatment of HeLa cells with insulin enhanced the phosphorylation as well as the Cr-induced degradation of the eIF4E protein. (cdc.gov)
  • In addition, site-directed mutation at serine 209 - the phosphorylation site required for activation of the eIF4E protein, abolished its degradation induced by Cr in HeLa cells. (cdc.gov)
  • These results confirmed that the phosphorylation of eIF4E protein is required for its ubiquitination and degradation in HeLa cells treated with Cr. (cdc.gov)
  • Similarly, the siRNA-mediated silencing of the eIF4E gene's expression in HeLa cells also resulted in a significant reduction in the cellular level of cyclin D1 protein. (cdc.gov)
  • X-ray crystallographic structures have become an important tool in modern drug experimental analysis of few drugs was carried out to test discovery process and insights can be obtained on the the inhibitory activity against carcinoma of cervix, HeLa protein-ligand interactions and biological function of cells. (medicinelakex1.com)
Line17
  • Lipofection was used to transiently transfect the hOCT1 plasmid DNA in a human cell line, HeLa. (aspetjournals.org)
  • He patented neither the HeLa cell line nor the roller drum he created to culture cells-either of which would have made him a multimillionaire-and at times had trouble making payments on his modest house. (shortform.com)
  • In this study we employed a mutational approach to define the nutrients and metabolic pathways required by Salmonella enterica serovar Typhimurium during infection of a human epithelial cell line (HeLa). (ox.ac.uk)
  • But her cells now had their own rooms at Johns Hopkins, and Gey was beginning to publish the fact that he'd found and perfected the line he called HeLa. (chrislombardi.me)
  • The National Institutes of Health (NIH) has reached a "unique" agreement with the descendants of a woman who gave the world HeLa cells, the most widely used cell line in the world. (medscape.com)
  • Dr Gey found that the cancerous cells had a remarkable innate ability to proliferate extensively within a laboratory environment, which resulted in the formation of the first human cell line [2,4]. (porterhousemedical.com)
  • The cancer cell line that biomedical researchers refer to as "HeLa" is so named because it was derived from the cervical cancer of a woman whose first and last names began with those letters. (jhu.edu)
  • Human cell line derived from cervical cancer. (riken.jp)
  • We also can send live cells to customers wishing to start working immediately with the line. (topogen.com)
  • Customers may request receiving a reporter cell line ready for culture (shipped at ambient temperature). (topogen.com)
  • the present investigation concluded that the extracted compound has shown to be a potent invitro anticancer activity against the human cervical cancer Hela cell line and docking studies. (derpharmachemica.com)
  • An immortal human cell line is a cluster of cells that continuously multiply on their own outside of the human from which they originated. (asu.edu)
  • Description: GFP expression stable cell line in Hela cells with Blasticidin marker. (choleratox.com)
  • Description: Luciferase (firefry) stable cell line with Blasticidin marker. (choleratox.com)
  • Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. (choleratox.com)
  • Data for analysis of YIF1B mRNA expression were downloaded from the website portals: The Cancer Genome Atlas (TCGA), GTEx, Cancer Cell Line Encyclopedia (CCLE) and International Cancer Genome Consortium (ICGC), including clinical and mutational information. (silverchair.com)
  • The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. (ucsd.edu)
Assay4
  • The cytotoxic effect of the drugs on HeLa cells was determined by an Alamar Blue assay. (pacific.edu)
  • In HeLa cell adherence assay, 48 [‎71%]‎ isolates exhibited mannose-resistant adherence to HeLa cells. (who.int)
  • Cell number needs to be optimized for each assay. (bdbiosciences.com)
  • Optimal assay conditions require a confluent monolayer of cells prior to the assay. (bdbiosciences.com)
Human cervical cancer cell1
  • 11 CaSki, a human cervical cancer cell collection, was obtained from American Type INCB018424 biological activity Culture Collection (Manassas, VA). CaSki monolayers were grown in RPMI-1640 medium with 10% FBS and 2.5 mmol/L L-glutamine. (irjs.info)
Mitochondria4
  • The RNase P associated with HeLa cell mitochondria contains an essential RNA component identical in sequence to that of the nuclear RNase P. (duke.edu)
  • In particular, these experiments revealed that a small fraction of the cell H1 RNA (of the order of 0.1 to 0.5%), calculated to correspond to approximately 33 to approximately 175 intact molecules per cell, is intrinsically associated with mitochondria and can be removed only by treatments which destroy the integrity of the organelles. (duke.edu)
  • In the same experiments, the use of a probe specific for the RNA component of RNase MRP showed the presence in mitochondria of 6 to 15 molecules of this RNA per cell. (duke.edu)
  • YFP-Parkin (green) does not translocate to mitochondria (red) when HeLa cells are transfected with control siRNA (siCtrl). (cellimagelibrary.org)
Antibody1
  • Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or SignalSilence ® mTOR siRNA I (+), using mTOR Antibody #2972 and eIF4B Antibody #3592. (cellsignal.com)
Genome5
  • The agreement was reached after months of talks, sparked by concerns about privacy after the entire HeLa genome was published online. (medscape.com)
  • The talks began in March 2013, after the Lacks family raised privacy concerns when the entire HeLa genome was published in G3: Genes, Genomes, Genetics . (medscape.com)
  • When they heard about the family's concerns, the German scientists who published the genome removed the HeLa sequence data from public access, and offered to work with the family on a mutually acceptable solution, according to a press release from the Genetics Society of America (GSA). (medscape.com)
  • We carried out haplotype-resolved whole-genome sequencing of the HeLa CCL-2 strain, examined point- and indel-mutation variations, mapped copy-number variations and loss of heterozygosity regions, and phased variants across full chromosome arms. (elsevier.com)
  • These data provide an extensively phased, high-quality reference genome for past and future experiments relying on HeLa, and demonstrate the value of haplotype resolution for characterizing cancer genomes and epigenomes. (elsevier.com)
Abstract1
  • Abstract: 'HeLa cells in monolayer cultures were treated with the following inhibitors of DNA synthesis: mitomycin C, nitrogen mustard, fluorodeoxyuridine, hydroxyurea, arabinofuranosylcytosine and high concentrations of thymidine. (harvard.edu)
Transfection4
  • You are working with fluorescence-labeled cells or specify transfection rates? (zeiss.com)
  • Fluorescent detection of SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency. (cellsignal.com)
  • CST recommends transfection with 100 nM mTOR siRNA I 48 hours prior to cell lysis. (cellsignal.com)
  • At 3 h after transfection of HeLa cells with the plasmid-type probes, fluorescence emission was detected at the nuclei by fluorescence microscopy only when the probe contained the (6-4) photoproduct, and the results were confirmed by flow cytometry. (osaka-u.ac.jp)
Plasmid3
  • 14 C-TEA uptake in hOCT1 plasmid DNA-transfected HeLa cells was trans -stimulated by unlabeled TEA and 1-methyl-4-phenyl-pyridinium. (aspetjournals.org)
  • HR is triggered by a DS break which is achieved by transfecting cells with an expression plasmid for I-Sce1 (Fig. 2). (topogen.com)
  • Erratum in Emerg Infect the EPEC adherence factor plasmid strain as well as in HEp-2 cells. (cdc.gov)
Adherence4
  • AmBisome inhibits the adherence of Candida albicans to HeLa cells" by Krystyna Konopka, Barbara Dorocka-Bobkowska et al. (pacific.edu)
  • Here we examined the effect of AmBisome, the liposomal AMB formulation, and free AMB on the adherence of C. albicans to HeLa cells. (pacific.edu)
  • Methods: The adherence of C. albicans to HeLa cells was determined as described by Samaranayake et al. (pacific.edu)
  • Conclusions: The liposomal AMB formulation, AmBisome, which is not toxic in a wide range of concentrations, inhibits candidal colonization when present during the "adherence phase", while the cell-associated Candida yeasts are highly resistant to antifungals in terms of adherence. (pacific.edu)
Chromosomes2
  • Integration sites of human papillomavirus 18 DNA sequences on HeLa cell chromosomes. (wikidata.org)
  • The DNA that makes up the chromosomes is duplicated (replicated) so that each new cell will get a complete set of chromosomes. (medlineplus.gov)
Tumor cells2
  • The comparisons of RCC1 levels in cell cycleCsynchronized HeLa and HFF-1 cells were obtained from data shown in Fig. HFF-1 cells created cells with steep mitotic RanGTP gradients much like HeLa cells, indicating that chromosomal gain can promote mitosis in aneuploid tumor cells via Went. (globaltechbiz.com)
  • Viral and mutant viral vectors expressing chemokines, genetically modified dendritic cells with chemokine or chemokine receptors, engineered chemokine-expressing tumor cells and pDNA encoding chemokines are among these methods. (medscape.com)
Morphology3
  • Microtubule experiments showed that DES inhibited spindleAssembly and disassembled the CMTC, whereas estradiol, at similar concentrations, arrested mitosis in a manner that allowed spindle assembly, and changes in cell morphology were observed. (semanticscholar.org)
  • In this study, the cell membrane morphology of living C2C12 (myoblast) and HeLa cells, as well as the dynamics of those membranes, were captured by SICM over time. (parksystems.com)
  • Morphology of HeLa cells in PF (7.5 mg/ml) microcarrier suspended in a bioreactor was examined by confocal microscopy (scale bar = 50μm). (ismicroscopy.org.il)
Cultures7
  • Prevention of postirradiation mitotic delay in cultures of L mouse cells by calcium salts. (semanticscholar.org)
  • I understand that HeLa cells have actually contaminated other cell cultures in labs because cells can float through the air and end up in another cell medium. (cancer.org)
  • The thinking was that cell cultures needed to be in constant motion, like blood and other fluids in the human body, in order for the cells to replicate. (shortform.com)
  • In its effort to standardize the recipe for culture medium and instructions for handling cell cultures, George Otto Gey's lab partnered with a small biological supply company named Microbiological Associates. (shortform.com)
  • Choose from all standard contrasting techniques, including DIC, to investigate your cell cultures. (zeiss.com)
  • Cultures were washed with phosphate-buffered saline, followed by 0.02% EDTA to remove the feeder cells. (irjs.info)
  • Syncytium formation shown by Nariva virus in BHK-21 and Vero cell cultures. (cdc.gov)
George Gey7
  • Learn more about Dr. George Gey, HeLa, and how his discovery impacted his life. (shortform.com)
  • After getting the tissue to George Gey, HeLa was made. (shortform.com)
  • With the roller drum created by George Gey, HeLa cells could be grown was possible. (shortform.com)
  • With the partnership with Dr. George Gey, HeLa distribution was underway. (shortform.com)
  • Dr. George Gey had developed effective and reproducible cell culturing and preservation techniques, and because of George Gey, HeLa cells were being sent all over the world. (shortform.com)
  • One colleague of Gey, however, remembers Dr. George Gey visiting Henrietta and telling Henrietta that her cells would live on after she died and help save countless lives. (shortform.com)
  • That specimen, Skloot explained, was sent somewhere having nothing to do with treatment: to cell biologist George Gey, inventor of the "roller drum" used in labs worldwide, who was in the process of gathering all the cervical-cancer cells he could find. (chrislombardi.me)
Monolayer2
  • Radiation-induced division delay in synchronized Chinese hamster ovary cells in monolayer culture. (semanticscholar.org)
  • Chinese hamster ovary cells in monolayer culture, synchronized by mechanically selecting mitotic cells in the absence of drugs, were x-irradiated with 150, 300, or 600 rads at frequent intervals du. (semanticscholar.org)
Tissue culture1
  • Even though RanGTP-regulated or RanGTP gradients had been recognized in meiotic egg components, maturing mouse oocytes, and tissue-culture cell lines (Kalb et al. (globaltechbiz.com)
Chromatin1
  • We conclude that TFIIA tau is associated with undifferentiated cells during development, yet is down-regulated at the chromatin level upon cellular differentiation. (nih.gov)
Control siRNA1
  • HeLa cells stably expressing YFP-Parkin were transfected with control siRNA f. (cellimagelibrary.org)
Membrane5
  • Especially importantly, imaging a cell membrane in high resolution and monitoring its dynamics can reveal a variety of cellular processes and cell signaling behavior. (parksystems.com)
  • To capture the real nature of cell membrane dynamics, it is important that the cell's physiology is not interrupted by imaging technique in use. (parksystems.com)
  • The continuous imaging of cell membranes unveils the detail morphological physiology of C2C12 and HeLa cells by showing outward cellular extensions, such as microviili, covering the plasma membrane. (parksystems.com)
  • It is clearly observed that the microvilli pops down crossing the cell membrane (C2C12), and those cellular extensions are transforming its structure and moving the position dynamically in the lateral direction as time passes (HeLa cell). (parksystems.com)
  • Physical Studies of Cell Surface and Cell Membrane Structure. (illinois.edu)
Significantly2
  • The susceptibility of cell-associated Candida to AMB and AmBisome was significantly lower. (pacific.edu)
  • They are also less sensitive to light than rod cells and are significantly less numerous. (microscopyu.com)
Strain1
  • The strain was produced in Vero E6 cells, and the passage 2 stock was obtained using plaque-forming assays. (socialworker-findoffice.com)
Genes3
  • Using S. Typhimurium strains deleted for genes encoding components of the phosphotransferase system and glucose transport, we show that glucose is a major substrate required for the intracellular replication of S. Typhimurium in HeLa cells. (ox.ac.uk)
  • We also deleted genes encoding enzymes involved in the utilization of gluconeogenic substrates and the glyoxylate shunt and show that neither of these pathways were required for intracellular replication of S. Typhimurium within HeLa cells. (ox.ac.uk)
  • This is a HeLa cell-based reporter kit designed to allow the customer to screen or identify agents (drugs, natural products, small molecules, synthetics, miRNAs, and genes) that affect or impact the process of HR DNA repair. (topogen.com)
Lacks3
  • He made a deal with Lacks' surgeon to use her tissue to make lasting cells. (shortform.com)
  • The Lacks family has taken an important step toward ensuring that HeLa cells continue to catalyze important advances in biomedical research," said Adam Fagen, PhD, executive director of GSA. (medscape.com)
  • In 2013, the National Institutes of Health (NIH) worked with the Lacks family to facilitate better relations and obtain consent to use the HeLa cells' genetic data. (inthewaitingroom.org)
Bacterial1
  • bacterial clusters on HeLa and HEp-2 is lacking. (cdc.gov)
Lysates3
  • Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
  • See here for more information on knockout cell lysates. (abcam.com)
  • Cell and tissue lysates come lyophilized for maximum stability and utility. (biocheminfo.org)
Mammalian4
  • Molecular growth requirements of single mammalian cells. (riken.jp)
  • The levels of General Transcription Factor (TF) IIA were examined during mammalian brain development and in rat embryo fibroblasts and transformed cell lines. (nih.gov)
  • They range from 0.1um to 1um in size and exist in most of mammalian cell types. (ucsd.edu)
  • The medium is specially designed by Harry Eagle to meet the specific nutritional requirements of certain subtypes of HeLa cells and normal mammalian fibroblasts. (biomall.in)
Humans2
  • Composed of many layers, the retina of humans and most vertebrates contains a stratum of rods and cones, the photosensitive cells of the eye. (microscopyu.com)
  • Scientists use immortal human cell lines in their research to investigate how cells function in humans. (asu.edu)
Compounds2
  • We tested the interaction of an array of organic cations and other compounds with hOCT1 by determining K i values in inhibiting 14 C-tetraethylammonium (TEA) transport in the transfected cells. (aspetjournals.org)
  • Compounds at 1 M were incubated with virus (Sabin 1) for 2 hours at 37°C. The virus-compound mixture was diluted 100-fold and added to HeLa cells at conditions previously described. (cdc.gov)
Cytoplasm1
  • Studzinski, GP 1964, ' Nucleolus-like inclusions in the cytoplasm of HeLa cells treated with puromycin [31] ', Nature , vol. 203, no. 4947, pp. 883-884. (researchwithnj.com)
Lines9
  • You know, I used to do a lot of science with these cell lines," Freedman said to this reporter just before the lecture. (chrislombardi.me)
  • HeLa cells have become one of the most frequently used cell lines, holding a pivotal role in many medical advances. (porterhousemedical.com)
  • HeLa cell lines have also been used to understand diseases such as those caused by HIV and the Ebola virus, where mechanisms of disease entry and transmission have been analysed to further develop understanding of pathogen modes of action [8,9]. (porterhousemedical.com)
  • Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. (wikidata.org)
  • Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines. (wikidata.org)
  • Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines. (wikidata.org)
  • Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins. (wikidata.org)
  • The large TFIIA subunit paralogues alphabeta and tau are largely produced in unsynchronized cell lines, yet only TFIIA alphabeta is observed in a number of differentiated tissue extracts. (nih.gov)
  • Probenecid, a standard inhibitor of nonspecific anion transport, is not required for dye-loading into CHO, Hela and BD™ ACTOne cell lines with this kit, but can improve retention of the dye in cells. (bdbiosciences.com)
Biomedical1
  • Additionally, the production of HeLa cells on an industrial scale for global distribution led to them inadvertently contribute towards the development of routine maintenance procedures and equipment for use in vaccination design, and also provided the foundation of the biomedical sample circulation industry [7]. (porterhousemedical.com)
20201
  • De Souza CEA, Andrade Pires AR, Cardoso CR, Carlos RM, Cadena SMSC, Acco A (2020) Antineoplastic activity of a novel ruthenium complex against human hepatocellular carcinoma (HepG2) and human cervical adenocarcinoma (HeLa) cells. (mitophysiology.org)
Fibroblasts1
  • Finally, XPA fibroblasts and the same cells expressing the XPA gene were transfected with the photoproduct-containing probe. (osaka-u.ac.jp)
Fluorescence3
  • A deconvolved wide-field fluorescence microscope image of human HeLa cancer cells undergoing cytokinesis (cell division). (wellcomecollection.org)
  • This method works on the principle that when a damaged strand is incised by NER, it is broken down by nucleolytic enzyme nuclease in cells and fluorophores separate from the quencher, yielding fluorescence. (osaka-u.ac.jp)
  • Although the transfer of the probe into the cells was slow, fluorescence was detected depending on the NER ability of the cells. (osaka-u.ac.jp)
Apoptotic5
  • The apoptotic cells were either stained with annexin V-FITC and propidium iodide (PI) and examined with flow cytometry, or subjected to Western blot. (biomedcentral.com)
  • A . The percentage of apoptotic cells was presented. (biomedcentral.com)
  • In the present study, the apoptotic effect of iron-free bovine lactoferrin (apo-bLf) on human epithelial cancer (HeLa) cells was examined in association with reactive oxygen species and glutathione (GSH) levels. (univaq.it)
  • Some experimental evidence shows that, methoxy substituted stilbenes are having good cancer chemo-preventive agent and most of methoxy resveratrol derivatives exhibit potent cytotoxic and pro-apoptotic activity against cancer cells [8-11]. (derpharmachemica.com)
  • against cell death mediated apoptotic protease enzyme volume of 100 µl and then cultured for 48 hr. (medicinelakex1.com)
0.052
  • 0.05 vs Hela and scramble control. (biomedcentral.com)
  • After 72 hour incubation incubation at 37°C, cells were stained with crystal violet (0.05% crystal violet, 0.5% Tween-20, 50% ethanol, in deionized H2O) and washed three times with deionized H2O. (cdc.gov)
Stable2
  • Hela cells and the stable variants were exposed to different concentration of cisplatin (CDDP) for 24 h. (biomedcentral.com)
  • We find that HeLa is relatively stable in terms of point variation, with few new mutations accumulating after early passaging. (elsevier.com)
Cancer cells4
  • It was the first successful attempt -after many such attempts- by cancer researchers to grow human cancer cells in a test tube. (cancer.org)
  • I wonder if other types of cancer cells are being grown in the research labs all over the world. (cancer.org)
  • To be exact, only her cancer cells continue to live but they may live for up to 100 years if frozen. (wordpress.com)
  • Physical properties of estrogen receptor complexes in MCF-7 human breast cancer cells. (illinois.edu)
Organism1
  • The robust growth and unrestricted distribution of HeLa cells resulted in its broad adoption - both intentionally and through widespread cross-contamination - and for the past 60 years it has served a role analogous to that of a model organism. (elsevier.com)
Infection1
  • Nutritional and metabolic requirements for the infection of HeLa cells by Salmonella enterica serovar Typhimurium. (ox.ac.uk)
Differentiation3
  • Although mesenchymal stem cells (MSCs) exhibit beneficial neural protective effects, their limited differentiation capacity in vivo attenuates their therapeutic function. (nature.com)
  • Hematopoiesis and Leukemia Laboratory, Research Unit on Cell Differentiation and Cancer, Faculty of High Studies Zaragoza, National Autonomous University of Mexico, Mexico City CP 09230, Mexico. (bvsalud.org)
  • After cultured in differentiation medium for 3 days, these cells differentiated i. (ucsd.edu)
Exposure2
  • My first exposure to HeLa cells occurred when I was a young student, and was told not to contaminate anything," Mitchell explained, "because, and I quote, 'Those HeLa cells will grow all over the place. (chrislombardi.me)
  • Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)
Mutation1
  • FDA Approval of Gefitinib for the Treatment of Patients with Metastatic EGFR Mutation-Positive Non-Small Cell Lung Cancer. (cdc.gov)
Toxicity2
  • Ubiquitination and degradation of eukaryotic translation initiation factor 4E result in toxicity and death in HeLa cells exposed to potassium dichromate. (cdc.gov)
  • The melittin-modified polymers transfected both HeLa and neuron-like PC-12 cells more efficiently than melittin-free polymers although toxicity associated with the melittin peptide was observed. (mst.edu)
Donor1
  • 2011), where the donor REF = 2,519 ps may be the mean donor of mTFP-1 indicated in cells within the lack of the acceptor (Fig. S1, F) and E. Open in another window Shape 1. (globaltechbiz.com)