HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Poliovirus: A species of ENTEROVIRUS which is the causal agent of POLIOMYELITIS in humans. Three serotypes (strains) exist. Transmission is by the fecal-oral route, pharyngeal secretions, or mechanical vector (flies). Vaccines with both inactivated and live attenuated virus have proven effective in immunizing against the infection.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.Adenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.Mitosis: A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Kinetics: The rate dynamics in chemical or physical systems.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Molecular Weight: The sum of the weight of all the atoms in a molecule.Ribonucleoproteins: Complexes of RNA-binding proteins with ribonucleic acids (RNA).Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Cell Extracts: Preparations of cell constituents or subcellular materials, isolates, or substances.Cytoplasm: The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Viral Proteins: Proteins found in any species of virus.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.DNA Replication: The process by which a DNA molecule is duplicated.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Recombinant Proteins: Proteins prepared by recombinant DNA technology.UridineMicroscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Cell Nucleolus: Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)RNA Interference: A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Rhinovirus: A genus of PICORNAVIRIDAE inhabiting primarily the respiratory tract of mammalian hosts. It includes over 100 human serotypes associated with the COMMON COLD.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.RNA Splicing: The ultimate exclusion of nonsense sequences or intervening sequences (introns) before the final RNA transcript is sent to the cytoplasm.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.RNA, Heterogeneous Nuclear: Nuclear nonribosomal RNA larger than about 1000 nucleotides, the mass of which is rapidly synthesized and degraded within the cell nucleus. Some heterogeneous nuclear RNA may be a precursor to mRNA. However, the great bulk of total hnRNA hybridizes with nuclear DNA rather than with mRNA.Interphase: The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Adenovirus Early Proteins: Proteins encoded by adenoviruses that are synthesized prior to, and in the absence of, viral DNA replication. The proteins are involved in both positive and negative regulation of expression in viral and cellular genes, and also affect the stability of viral mRNA. Some are also involved in oncogenic transformation.Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)RNA Precursors: RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Vaccinia virus: The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Dactinomycin: A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)Luminescent Proteins: Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.Ribonucleoproteins, Small Nuclear: Highly conserved nuclear RNA-protein complexes that function in RNA processing in the nucleus, including pre-mRNA splicing and pre-mRNA 3'-end processing in the nucleoplasm, and pre-rRNA processing in the nucleolus (see RIBONUCLEOPROTEINS, SMALL NUCLEOLAR).Enterovirus B, Human: A species of ENTEROVIRUS infecting humans and containing 36 serotypes. It is comprised of all the echoviruses and a few coxsackieviruses, including all of those previously named coxsackievirus B.Heterogeneous-Nuclear Ribonucleoproteins: A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.RNA-Binding Proteins: Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Cell-Free System: A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)Cytotoxins: Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.TritiumGenes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Cycloheximide: Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.RNA Cap-Binding Proteins: Proteins that specifically bind to RNA CAPS and form nuclear cap binding protein complexes. In addition to stabilizing the 5' end of mRNAs, they serve a diverse array of functions such as enhancing mRNA transport out of the CELL NUCLEUS and regulating MRNA TRANSLATION in the CYTOPLASM.Chloramphenicol O-Acetyltransferase: An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.Shigella flexneri: A bacterium which is one of the etiologic agents of bacillary dysentery (DYSENTERY, BACILLARY) and sometimes of infantile gastroenteritis.Precipitin Tests: Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Mitochondria: Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.RNA, Small Nuclear: Short chains of RNA (100-300 nucleotides long) that are abundant in the nucleus and usually complexed with proteins in snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR). Many function in the processing of messenger RNA precursors. Others, the snoRNAs (RNA, SMALL NUCLEOLAR), are involved with the processing of ribosomal RNA precursors.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Tissue Culture Techniques: A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.Regulatory Sequences, Nucleic Acid: Nucleic acid sequences involved in regulating the expression of genes.

The incorporation of 5-iodo-2'-deoxyuridine into the DNA of HeLa cells and the induction of alkaline phosphatase activity. (1/37241)

Inhibition of DNA synthesis during the period of exposure of HeLa cells to 5-iodo-2'-deoxyuridine (IUdR) inhibited the induction of alkaline phosphatase activity. This finding, taken together with previous findings that IUdR did not induce alkaline phosphatase activity in the presence of 2-fold molar excess thymidinemonstrated that IUdR incorporation into DNA is correlated with the increase in alkaline phosphatase activity. With the exception of an interim period described in the text, induction of alkaline phosphatase activity was linearly related to medium concentrations of IUdR of up to at least 3 muM. However, the extent of IUdR substitution in DNA did not appear to be related to the degree of enzyme induction. Alkaline phosphatase activity continued to increase at medium concentrations of IUdR from 1 to 3 muM, while little further substitution of DNA occurred.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (2/37241)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Diphtheria toxin effects on human cells in tissue culture. (3/37241)

HeLa cells exposed to a single sublethal concentration of diphtheria toxin were found to have diminished sensitivity when subsequently reexposed to the toxin. Three cells strains exhibiting toxin resistance were developed. In the cells that had previously been exposed to toxin at 0.015 mug/ml, 50% inhibition of protein synthesis required a toxin concentration of 0.3 mug/ml, which is more than 10 times that required in normal HeLa cells. There appears to be a threshold level of diphtheria toxin action. Concentrations of toxin greater than that required for 50% inhibition of protein synthesis (0.01 mug/ml) are associated with cytotoxicity, whereas those below this concentration may not be lethal. Several established human cell lines of both normal and neoplastic origin were tested for their sensitivity to the effects of the toxin. No special sensitivity was observed with the cells of tumor origin. Fifty % inhibition of protein synthesis of HeLa cells was achieved with diphtheria toxin (0.01 mug/ml) as compared to the normal human cell lines tested (0.03 and 0.5 mug/ml) and a cell line derived from a human pancreatic adenocarcinoma (0.2 mug/ml). A human breast carcinoma cell line showed a maximum of 45% inhibition of protein synthesis. This required a diphtheria toxin concentration of 5 mug/ml. These results suggest that different human cell lines show wide variation in their sensitivity to the toxin.  (+info)

Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite. (4/37241)

The reaction of HeLa-cell 5.8S rRNA with NaHSO3 under conditions in which exposed cytidine residues are deaminated to uridine was studied. It was possible to estimate the reactivities of most of the 46 cytidine residues in the nucleotide sequence by comparing 'fingerprints' of the bisulphite-treated RNA with those of untreated RNA. The findings were consistent with the main features of the secondary-structure model for mammalian 5.85S rRNA proposed by Nazar, Sitz, & Busch [J. Biol. Chem (1975) 250, 8591--8597]. Five out of six regions that are depicted in the model as single-stranded loops contain cytidine residues that are reactive towards bisulphite at 25 degrees C (the other loop contains no cytidine). The cytidine residue nearest to the 3'-terminus is also reactive. Several cytidines residues that are internally located within proposed double-helical regions show little or no reactivity towards bisulphite, but the cytidine residues of several C.G pairs at the ends of helical regions show some reactivity, and one of the proposed loops appears to contain six nucleotides, rather than the minimum of four suggested by the primary structure. Two cytidine residues that are thought to be 'looped out' by small helix imperfections also show some reactivity.  (+info)

Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. (5/37241)

Studies of the actin-based motility of pathogens have provided important insights into the events occurring at the leading edge of motile cells [1] [2] [3]. To date, several actin-cytoskeleton-associated proteins have been implicated in the motility of Listeria or Shigella: vasodilator-stimulated phosphoprotein (VASP), vinculin and the actin-related protein complex of Arp2 and Arp3 [4] [5] [6] [7]. To further investigate the underlying mechanism of actin-tail assembly, we examined the localization of components of the actin cytoskeleton including Arp3, VASP, vinculin and zyxin during vaccinia, Listeria and Shigella infections. The most striking difference between the systems was that a phosphotyrosine signal was observed only at the site of vaccinia actin-tail assembly. Micro-injection experiments demonstrated that a phosphotyrosine protein plays an important role in vaccinia actin-tail formation. In addition, we observed a phosphotyrosine signal on clathrin-coated vesicles that have associated actin-tail-like structures and on endogenous vesicles in Xenopus egg extracts which are able to nucleate actin tails [8] [9]. Our observations indicate that a host phosphotyrosine protein is required for the nucleation of actin filaments by vaccinia and suggest that this phosphoprotein might be associated with cellular membranes that can nucleate actin.  (+info)

Bcl-2 regulates amplification of caspase activation by cytochrome c. (6/37241)

Caspases, a family of specific proteases, have central roles in apoptosis [1]. Caspase activation in response to diverse apoptotic stimuli involves the relocalisation of cytochrome c from mitochondria to the cytoplasm where it stimulates the proteolytic processing of caspase precursors. Cytochrome c release is controlled by members of the Bcl-2 family of apoptosis regulators [2] [3]. The anti-apoptotic members Bcl-2 and Bcl-xL may also control caspase activation independently of cytochrome c relocalisation or may inhibit a positive feedback mechanism [4] [5] [6] [7]. Here, we investigate the role of Bcl-2 family proteins in the regulation of caspase activation using a model cell-free system. We found that Bcl-2 and Bcl-xL set a threshold in the amount of cytochrome c required to activate caspases, even in soluble extracts lacking mitochondria. Addition of dATP (which stimulates the procaspase-processing factor Apaf-1 [8] [9]) overcame inhibition of caspase activation by Bcl-2, but did not prevent the control of cytochrome c release from mitochondria by Bcl-2. Cytochrome c release was accelerated by active caspase-3 and this positive feedback was negatively regulated by Bcl-2. These results provide evidence for a mechanism to amplify caspase activation that is suppressed at several distinct steps by Bcl-2, even after cytochrome c is released from mitochondria.  (+info)

The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. (7/37241)

The cellular protein p32 was isolated originally as a protein tightly associated with the essential splicing factor ASF/SF2 during its purification from HeLa cells. ASF/SF2 is a member of the SR family of splicing factors, which stimulate constitutive splicing and regulate alternative RNA splicing in a positive or negative fashion, depending on where on the pre-mRNA they bind. Here we present evidence that p32 interacts with ASF/SF2 and SRp30c, another member of the SR protein family. We further show that p32 inhibits ASF/SF2 function as both a splicing enhancer and splicing repressor protein by preventing stable ASF/SF2 interaction with RNA, but p32 does not block SRp30c function. ASF/SF2 is highly phosphorylated in vivo, a modification required for stable RNA binding and protein-protein interaction during spliceosome formation, and this phosphorylation, either through HeLa nuclear extracts or through specific SR protein kinases, is inhibited by p32. Our results suggest that p32 functions as an ASF/SF2 inhibitory factor, regulating ASF/SF2 RNA binding and phosphorylation. These findings place p32 into a new group of proteins that control RNA splicing by sequestering an essential RNA splicing factor into an inhibitory complex.  (+info)

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (8/37241)

Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. A stable complex containing both Zta and CBP could be isolated from lytically stimulated, but not latently infected RAJI nuclear extracts. Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1ADelta2-36), which fails to bind CBP. Zta bound directly to two related cysteine- and histidine-rich domains of CBP, referred to as C/H1 and C/H3. These domains both interacted specifically with the transcriptional activation domain of Zta in an electrophoretic mobility shift assay. Interestingly, we found that the C/H3 domain was a potent dominant negative inhibitor of Zta transcriptional activation function. In contrast, an amino-terminal fragment containing the C/H1 domain was sufficient for coactivation of Zta transcription and viral reactivation function. Thus, CBP can stimulate the transcription of latent EBV in a histone acetyltransferase-independent manner mediated by the CBP amino-terminal C/H1-containing domain. We propose that CBP may regulate aspects of EBV latency and reactivation by integrating cellular signals mediated by competitive interactions between C/H1, C/H3, and the Zta activation domain.  (+info)

*BUB1

In vitro knockdown of Bub1 in p53 impaired cells (e.g. HeLa cells) caused aneuploidy. Whether aneuploidy alone is a sufficient ... In humans Bub1 accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after ... Cell. 32 (3): 394-405. doi:10.1016/j.molcel.2008.09.017. PMC 2644263 . PMID 18995837. UniProt: O43683 "BUB1 protein (Homo ... Jeganathan K, Malureanu L, Baker DJ, Abraham SC, van Deursen JM (Oct 2007). "Bub1 mediates cell death in response to chromosome ...

*List of Stuff You Should Know episodes (2013)

How HeLa Cells Work. HowStuffWorks. Retrieved 2016-03-17. How Underwater Tunnels Work. HowStuffWorks. Retrieved 2016-03-17. How ...

*Katarína Horáková

Jantovà, S.; Horàkovà, K. (1993). "9-Hydroxybenfluron induced inhibition of proliferation and metabolism in hela cells". Cell ... "Degeneration of HeLa-cells induced by the cytotoxic effect of isothiocyanates or by the starvation of cells". Acta morphologica ... 2. Effect of l-alpha-ethyl ester of 2-isothiocyanate propionic acid on hela cell division and metabolism]. Biológia (in Slovak ... Horáková, K; Navarová, J; Nemec, P; Kettner, M (1974). "Effect of dactylarin of HeLa cells". The Journal of Antibiotics. 27 (6 ...

*Agrobacterium

Kunik, T.; Tzfira, T; Kapulnik, Y; Gafni, Y; Dingwall, C; Citovsky, V (2001). "Genetic transformation of HeLa cells by ... By altering the hormone balance in the plant cell, the division of those cells cannot be controlled by the plant, and tumors ... The plasmid T-DNA is integrated semi-randomly into the genome of the host cell, and the tumor morphology genes on the T-DNA are ... Agrobacterium attaches to and genetically transforms several types of human cells by integrating its T-DNA into the human cell ...

*Agrobacterium tumefaciens

"Genetic transformation of HeLa cells by Agrobacterium". Proceedings of the National Academy of Sciences. 98 (4): 1871-1876. doi ... Jun 1977). "Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis". Cell ... This stimulates cell proliferation and gall formation. The T-DNA contains genes for encoding enzymes that cause the plant to ... The Ti plasmid integrates a segment of its DNA, known as T-DNA, into the chromosomal DNA of its host plant cells. A. ...

*OSBPL11

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. ...

*Osmotic shock

Olivero P, Stutzin A (2004). "Calcium modulates osmosensitive taurine efflux in HeLa cells". Neurochem Res. 29 (1): 169-76. doi ... This also inhibits the transport of substrates and cofactors into the cell thus "shocking" the cell. Alternatively, at low ... Osmotic shock or osmotic stress is physiologic dysfunction caused by a sudden change in the solute concentration around a cell ... Current research also suggests that osmotic stress in cells and tissues may significantly contribute to many human diseases. In ...

*SNRPA1

Ohno M; Kataoka N; Shimura Y (1991). "A nuclear cap binding protein from HeLa cells". Nucleic Acids Res. 18 (23): 6989-95. doi: ...

*Chlorphenamine

Arias-Montaño JA, Young JM (1993). "Characteristics of histamine H1 receptors on HeLa cells". Eur. J. Pharmacol. 245 (3): 291-5 ... "Stable expression of human H1-histamine-receptor cDNA in Chinese hamster ovary cells. Pharmacological characterisation of the ...

*RNASEH2B

2) Ribonucleotides in mtDNA (Mouse and HeLa cells). Low levels of ribonucleotides incorporation in the nuclear genome may be ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Cell. 149 (5): 1008-22. doi:10.1016/j.cell.2012.04.011. PMC 3383994 . PMID 22579044. Chon H, Vassilev A, DePamphilis ML, Zhao Y ... 1.000.000 sites per cell, making it the most common endogenous base lesion in the mammalian genome. These lesions are best ...

*MCM6

Holthoff HP, Baack M, Richter A, Ritzi M, Knippers R (1998). "Human protein MCM6 on HeLa cell chromatin". J. Biol. Chem. 273 ( ... Cell. Biol. 19 (12): 8003-15. PMC 84885 . PMID 10567526. Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H (September 1996). " ... Cell. Biol. 19 (12): 8003-15. PMC 84885 . PMID 10567526. Ishimi Y, Komamura-Kohno Y, You Z, Omori A, Kitagawa M (2000). " ... Ohtani K, Iwanaga R, Nakamura M, Ikeda M, Yabuta N, Tsuruga H, Nojima H (1999). "Cell growth-regulated expression of mammalian ...

*LIG4

Robins P, Lindahl T (September 1996). "DNA ligase IV from HeLa cell nuclei". The Journal of Biological Chemistry. 271 (39): ... This interaction stabilizes LIG4 protein in cells; cells that are deficient for XRCC4, such as XR-1 cells, have reduced levels ... variable degrees of immunodeficiency and reduced number of blood cells. Accumulation of DNA damage leading to stem cell ... The sensitivity of haematopoietic stem cells to lig4 deficiency suggests that lig4-mediated NHEJ is a key determinant of the ...

*DBR1

Arenas J, Hurwitz J (1987). "Purification of a RNA debranching activity from HeLa cells". J. Biol. Chem. 262 (9): 4274-9. PMID ... 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635-48. doi:10.1016/ ...

*TXNIP

Joguchi A, Otsuka I, Minagawa S, Suzuki T, Fujii M, Ayusawa D (Apr 2002). "Overexpression of VDUP1 mRNA sensitizes HeLa cells ... 25-dihydorxyvitamin D3 inhibits tumor cell growth by blocking cell-cycle progression". Oncogene. 22 (26): 4035-46. doi:10.1038/ ... 25-dihydorxyvitamin D3 inhibits tumor cell growth by blocking cell-cycle progression". Oncogene. 22 (26): 4035-46. doi:10.1038/ ... "Isolation and characterization of a novel cDNA from HL-60 cells treated with 1,25-dihydroxyvitamin D-3". Biochimica et ...

*PTEN (gene)

"PTEN associates with the vault particles in HeLa cells". The Journal of Biological Chemistry. 277 (43): 40247-52. doi:10.1074/ ... The defective protein allows the cell to divide in an uncontrolled way and prevents damaged cells from dying, which can lead to ... The defective protein is unable to stop cell division or signal abnormal cells to die, which can lead to tumor growth, ... This phosphatase is involved in the regulation of the cell cycle, preventing cells from growing and dividing too rapidly. It is ...

*DNA polymerase alpha catalytic subunit

"On the association of DNA polymerase alpha activity with the nuclear matrix in HeLa cells". Cell Biol. Int. Rep. 15 (2): 131-40 ... Jackson DA, Cook PR (1987). "Different populations of DNA polymerase alpha in HeLa cells". J. Mol. Biol. 192 (1): 77-86. doi: ... sequences controlling expression in cycling and serum-stimulated cells". Mol. Cell. Biol. 11 (4): 2081-95. PMC 359896 . PMID ... Cell. Biol. 11 (4): 2108-15. PMC 359898 . PMID 1848671. Martelli AM, Cocco L, Manzoli FA (1991). " ...

*Major vault protein

"PTEN associates with the vault particles in HeLa cells". The Journal of Biological Chemistry. 277 (43): 40247-52. doi:10.1074/ ... "PTEN associates with the vault particles in HeLa cells". The Journal of Biological Chemistry. 277 (43): 40247-52. doi:10.1074/ ... "Adult T-cell leukemia cells over-express the multidrug-resistance-protein (MRP) and lung-resistance-protein (LRP) genes". ... "Interaction of vault particles with estrogen receptor in the MCF-7 breast cancer cell". The Journal of Cell Biology. 141 (6): ...

*DDX46

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi: ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. ...

*SFRS6

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Lemaire R, Winne A, Sarkissian M, Lafyatis R (March 1999). "SF2 and SRp55 regulation of CD45 exon 4 skipping during T cell ... Gui JF, Lane WS, Fu XD (June 1994). "A serine kinase regulates intracellular localization of splicing factors in the cell cycle ... Molecular Cell. 6 (2): 307-16. doi:10.1016/S1097-2765(00)00031-9. PMID 10983978. Fossey SC, Mychaleckyj JC, Pendleton JK, ...

*NOL1

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi: ... Cell Biol. 4 (7): 529-33. doi:10.1038/ncb814. PMID 12080348. Holsinger LJ, Ward K, Duffield B, Zachwieja J, Jallal B (2002). " ... Cell. 13 (11): 4100-9. doi:10.1091/mbc.E02-05-0271. PMC 133617 . PMID 12429849. Beausoleil SA, Jedrychowski M, Schwartz D, ... The protein encoded by this gene is a nucleolar antigen expressed in proliferating cells. It is not detectable in non- ...

*SF3B1

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Molecular Cell. 5 (5): 779-87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114. Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune ...

*RBMX

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Cell. 154 (2): 452-64. doi:10.1016/j.cell.2013.06.022. PMC 3717207 . PMID 23870131. "Infection and Immunity Immunophenotyping ( ... "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity ...

*NFIA

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. Lu W, Quintero-Rivera F, Fan Y, Alkuraya FS, Donovan DJ, ... Cell. 123 (5): 819-31. doi:10.1016/j.cell.2005.09.023. PMID 16325577. Lin YL, Wang YH, Lee HJ (November 2006). "Transcriptional ...

*Cyclin T2

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. doi: ... "Abrogation of signal-dependent activation of the cdk9/cyclin T2a complex in human RD rhabdomyosarcoma cells". Cell Death Differ ... Cell. 19 (4): 523-34. doi:10.1016/j.molcel.2005.06.027. PMID 16109376. Cottone G, Baldi A, Palescandolo E, Manente L, Penta R, ... Cell. Biol. 23 (14): 4859-69. doi:10.1128/MCB.23.14.4859-4869.2003. PMC 162212 . PMID 12832472. Beausoleil SA, Jedrychowski M, ...

*PALB2

"Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the ... This reduced fertility appears to be due to germ cell attrition resulting from a combination of unrepaired DNA breaks during ... Molecular Cell. 22 (6): 719-29. doi:10.1016/j.molcel.2006.05.022. PMID 16793542. Xia B, Dorsman JC, Ameziane N, de Vries Y, ... Molecular Cell. 22 (6): 719-29. doi:10.1016/j.molcel.2006.05.022. PMID 16793542. Rahman N, Seal S, Thompson D, Kelly P, Renwick ...

*Cervical cancer

In medical research, the most famous immortalised cell line, known as HeLa, was developed from cervical cancer cells of a woman ... First successful in-vitro cell line, HeLa, derived from biopsy of cervical cancer of Henrietta Lacks 1976 - Harald zur Hausen ... adenosquamous carcinoma small cell carcinoma neuroendocrine tumour glassy cell carcinoma villoglandular adenocarcinoma ... It is due to the abnormal growth of cells that have the ability to invade or spread to other parts of the body. Early on, ...
HeLa cancer cells. Immunofluorescent light micrograph of cultured HeLa cancer cells. The nucleus of each cell is pink, cytoplasm is stained red and green fibres of the protein tubulin are seen around each nucleus. Tubulin is used during cell division to divide chromosomes. HeLa cells are the first human cell line, established in 1952 to research cancer. They were obtained from the cervix of Henrietta Lacks, who died of cervical cancer. HeLa cancer cells are used in research worldwide. Immunofluorescence is a staining technique which uses antibodies to attach fluorescent dyes to specific molecules within cells. Magnification: x125 at 6x4.5cm size. - Stock Image G442/0071
Hypoxia plays a key role in tumour cell survival, invasion, and metastasis. An increasing number of studies have attempted to characterize the tumour response to hypoxia and to identify predictive markers of disease. Here we show that hypoxia increases tumour cell invasion and migration by the modulation of Rab11, an important molecule for vesicular trafficking. In our study, we found that Rab11, together with the activation of Rac1, could stimulate invasion and migration of cervical cancer cell lines HeLa/SiHa in hypoxia. Activation of Rac1 activity by hypoxia seems to be central to carcinoma invasion. We also found that these effects could be related to the integrin αvβ3. In addition, we studied the molecular pathway for this process. Our results showed that in cervical cancer cell... lines HeLa/SiHa, Rac1 activation in hypoxia could stimulate invasion and migration, and this process was mediated by integrin αvβ3-mediated FAK and PI3K phosphorylation. Furthermore, hypoxia induced a ...
BioAssay record AID 87350 submitted by ChEMBL: Compound was tested for antiviral activity in HeLa Cell cultures against herpes simplex virus type 1 (HSV-1).
HeLa cells in culture. Light microscopy footage of the movement, division and death of HeLa cervical cancer cells. HeLa cells are a continuously cultured cell line of human cancer cells. They are immortal, in that they can divide an unlimited number of times. However, they are also poorly differentiated and lack some of the characteristics of normal cells. HeLa cells are widely used in biological and medical research. They all originate from a sample taken in 1951 from cervical cancer patient Henrietta Lacks, after whom they are named. - Stock Video Clip K005/6160
BioAssay record AID 506767 submitted by ChEMBL: Binding affinity to SAP145 in SAP145-targeting siRNA-treated human HeLa cells at 1 uM by fluorescent microscopy.
HeLa cells are the worlds most commonly used human cell lines for research. In a study published today in G3: Genes, Genomes and Genetics online, scientists at EMBL announce the successful sequencing of the genome of a HeLa cell line. It provides a high-resolution genomic reference that reveals the striking differences between the HeLa genome and that of normal human cells. The study could improve the way HeLa cells are used to model human biology.
Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for tumor initiation and maintenance. They are also considered as an attractive target for advanced cancer therapy. Using a sphere culture method that favors the growth of self-renewal cells, we have isolated sphere-forming cells (SFCs) from cervical cancer cell lines HeLa and SiHa. HeLa-SFCs were resistant to multiple chemotherapeutic drugs and were more tumorigenic, as evidenced by the growth of tumors following injection of immunodeficient mice with 1 × 104 cells, compared with 1 × 106 parental HeLa cells required to grow tumors of similar size in the same time frame. These cells showed an expression pattern of CD44high/CD24low that resembles the CSC surface biomarker of breast cancer. We further demonstrated that HeLa-SFCs expressed a higher level (6.9-fold) of the human papillomavirus oncogene E6, compared with that of parental HeLa cells. Gene silencing of E6 with a lentiviral-short-hairpin RNA (shRNA)
Fig. 6. Mitotic events of cell division after disruption of mitochondria in the histone EGFP-H1 expressed cell. The disruption of a single mitochondrion by femtosecond laser irradiation had no influence on cell division or cell activity. The cell nuclei and mitotic chromosomes in HeLa cells were visualized using histone EGFP-H1. Mitochondria were stained with MitoTracker Red. Confocal fluorescence image and transmission image (a) before and (b) after femtosecond laser irradiation with 0.39 nJ/pulse (exposure time: 32 ms). The yellow arrow indicates the irradiation point. (c)-(f) Time-lapse confocal images and transmission images. The mitotic events of cell division in the irradiated cells proceeded normally. Scale bar: 20 μm ...
Im pretty sure the fact that HeLa cells are abnormal has been known more or less since they were first isolated. That said, how they are abnormal has not. And this is very relevant - if researchers are using HeLa cells to study something, and HeLa cells are abnormal along that axis, then their data are suspect. So knowing how they are normal, and abnormal, is important information. This is why the news that the transformation of healthy non-cancerous cervical cells into HeLa cells happened due to human papilloma virus infection - that is, Henrietta Lacks (the source of the cells) had HPV, which gave her the cancer that ultimately killed her. This is noteworthy because it establishes the mutation(s) and phenotype(s) that result when cervical cells are infected with HPV (with ramifications both for work with HeLa and other cervical cancer lines AND for care for patients).. Remember also that HeLa was the very first immortalized cell line. Studies on HeLa led to the immortalization of other cells, ...
Light micrograph of dividing HeLa cells. Chromosomes in the cell nucleus (purple), microtubules in the cell cytoskeleton (tubulin; green), and actin (red) are visible here. In the cell in the centre of the image, condensed chromosomes (purple) have aligned and attached to the spindle (green). During anaphase (one of the stages of nuclear division in mitosis), the spindle pulls the condensed chromosomes to opposite poles of the cell. The cell will finish dividing its nuclear material and then cytoplasm in order to physically separate into two daughter cells. HeLa cells are an immortal human epithelial cell line derived from a cancerous tumour of the cervix (adenocarcinoma). It was established in 1951 from a biopsy taken from Henrietta Lacks and was the first human cell line to survive and grow in the laboratory. Henriettas cells were originally used in this way without permission from her or her family which raises issues about ethics and privacy. HeLa cells have been used extensively around the world
Definition of HeLa cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is HeLa cell? Meaning of HeLa cell as a legal term. What does HeLa cell mean in law?
This study demonstrated the cytotoxic and cell growth inhibitory effect of a bioactive extract of |i|Funalia trogii|/i| (Berk.) Bond. et Singer, which was grown...
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
Cell and Culture Conditions. The PKR wild-type MEFs [MEF/PKR(+/+)] and PKR-knockout MEFs [MEF/PKR(-/-)], provided by Dr. Glen Barber (University of Miami, Miami, FL), have been described previously (Yang et al., 1995). The human cervical cancer cell line HeLa and the human lung cancer cell lines H1299, H460, H226B, and A549 were maintained in our laboratory. The human bronchial epithelial (HBE) cell line was purchased from Clonetics (Walkersville, MD). All cells except for HBE cells were routinely cultured in Dulbeccos modified Eagles medium supplemented with 10% heat-inactivated fetal calf serum, 100 units/ml penicillin, and 100 mg/ml streptomycin and maintained in the presence of 5% CO2 at 37°C. HBE cells were cultured in serum-free keratinocyte medium (Invitrogen, Carlsbad, CA).. Chemicals and Antibodies. A chemical library with 10,000 compounds, including BEPP and its analog BECC, was obtained from ChemBridge Research Laboratories, Inc.. The chemicals in the library were provided at a ...
One cryopreserved vial of HeLa NucLight Red (nuclear-labelled RFP) expressing cells at a density of 1x106 cells/vial. Each vial contains a stable population of 1 million HeLa cells expressing the NucLight Red fluorescent protein restricted to the nucleus. Parental HeLa cells were purchased from ATCC (Cat# CCL-2). HeLa
Although it is widely believed that eukaryotic DNA is looped by attachment to a nucleoskeleton, there is controversy about its composition and which sequences are attached to it. As most nuclear derivatives are isolated using unphysiological conditions, the criticism that attachments seen in vitro are generated artifactually has been difficult to rebut. Therefore we have re-investigated attachments of chromatin to the skeleton using physiological conditions. HeLa cells are encapsulated in agarose microbeads and lysed using Triton in a physiological buffer. Then, most chromatin can be electroeluted after treatment with a restriction enzyme to leave some at the base of the loops still attached. Analysis of the size and amounts of these residual fragments indicates that the loops are 80-90kbp long. The residual fragments are stably attached, with about 1kbp of each fragment protected from nuclease attack. This is very much longer than a typical protein-binding site of 10-20bp.
While both the Rabenosyn-5 and Vps45 orthologs appear to coordinate endocytic regulatory functions in yeast and invertebrates, their relationship in mammalian cells is not well understood. Leaving out -L would be equivalent to L0, or specifying that the pool level was backup01. To assess the impact of TBC1d5 loss on VPS35 recruitment to endosomes, TBC1d5 KO cells were mixed with control HeLa cells at a ratio of 1:1 and the cells were simultaneously stained with TBC1d5, VPS35 and EEA1. 1 ). This approach, used by most system emulators, allows the emulator to run an arbitrary guest operating system without modifications because guest OS is not aware that it is not running on real hardware. This is consistent with a proposed role for the vps pathway in VP40 vesicular release. Untuk konsultasi konfigurasi server custom isp for a dns server mendapatkan diskon terbaik (untuk pembelian cloud resources dalam jumlah banyak), silahkan hubungi free terraria server download. BlueHost grew enormously in the ...
INA-UV induced apoptosis in Hela cells is also caspase dependent. A. Hela cells, pre-incubated or not with 40 μM ZVADfmk were treated with indicated amounts of
HeLa cells were washed with cold PBS and lysed in cell lysis buffer (20-mM Tris-HCl, pH 8.0, 150-mM NaCl, 2-mM EGTA, 0.5-mM EDTA, 0.5% NP-40, 5-mM NaF, 1-mM Na3VO4, 1-mM PMSF, and 500× protease inhibitor cocktail; Calbiochem) for 20 min on ice. For Usp16/Plk1 coIP assays, cell lysates were mixed with anti-Usp16 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Usp16 1-257 aa) or mouse anti-Plk1 antibodies (06-813; EMD Millipore) and 15 µl protein A-Sepharose beads (75% slurry) and incubated at 4°C for 1 h. After washing with lysis buffer, the beads were harvested and suspended in Laemmli sample buffer. For Plk1/BubR1 coIP assays, cell lysates were mixed with anti-Plk1 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Plk1 326-end). For GST pull-down assays, HeLa cell lysates were incubated with 5 µg of soluble GST or GST-fused proteins bound to 15 µl glutathione-Sepharose beads (75% slurry) at 4°C for 1 h. After washing with lysis buffer, ...
Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options. Just like model […]. The post Top 5 of the most commonly used cell lines! appeared first on Bitesize Bio.. ...
AC0206: Western Blot (1:1000) of over-expressed TMX with positive and negative controls on HeLa Cells. TMX is the 34kDa band. The upper band (~38 kDa) is myc-tagged TMX. The lower band at~28 kDa (*) is a non-specific band.. ...
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Kinetics of B-fragment transport from EE/RE to the Golgi apparatus. (A) Confocal microscopy on living HeLa cells. Fluorophore-labeled B-fragment was internalize
Here we present a new approach to aqueous hydrazoic acid detection through the synthesis and evaluation of an alkyne-based fluorescent probe, which could be applied in the monitoring of hydrazoic acid in both living Hela cells and larval zebrafish. This probe can also serve as an early warning automaton whic
If you have a question about this talk, please contact Florian Markowetz.. Engineered biological systems that integrate multi-input sensing, sophisticated information processing, and precisely regulated actuation in living cells could be useful in a variety of applications. For example, anticancer therapies could be engineered to detect and respond to complex cellular conditions in individual cells with high specificity.. A few years ago we proposed a geenral-purpose approach to logic signal integration in mammalian cells using RNA interference. More recently, we applied these ideas to design a scalable transcriptional/posttranscriptional synthetic regulatory circuit-a cell-type "classifier"-that senses expression levels of a customizable set of endogenous microRNAs and triggers a cellular response only if the expression levels match a predetermined profile of interest. We demonstrate that a HeLa cancer cell classifier selectively identifies HeLa cells and triggers apoptosis without affecting ...
目的:探讨反义脱氧寡核苷酸封闭HSP70基因对体外宫颈癌 HeLa 细胞增殖、凋亡及化疗敏感性的影响。方法:1)将体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、反义寡核苷酸处理组(AS组)、正义寡核苷酸处理组(S组)、随机寡核苷酸处理组(R组),每组各5例,分别转染体外培养的宫颈癌 HeLa 细胞,采用Western免疫印迹检测各组细胞HSP70蛋白表达。2)将顺铂处理体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、单纯顺铂处理组(Cis组)、反义寡核苷酸+顺铂处理组(AS +Cis组)、正义寡核苷酸+顺铂处理组(S +Cis组)、随机寡核苷酸+顺铂处理组 (R +Cis组),每组各8例。采用四甲基偶氮唑蓝光吸收法(methyl thiazolyl tetrazolium,MTT) 法检测 HeLa 细胞的生长抑制率; 流式细胞术检测 HeLa 细胞的凋亡率。结果:1)Ctrl组、AS组、S组、R组HSP70灰度比值分别为1.365±0.187,0.379±0.134,1.403±0.163和1.410±0
When Professor Gertrude Buehring steps into her lab in Koshland Hall, she is never met by HeLa cells. In fact, she makes a point of it. "We never grow them," she says. "I wouldnt want to take that risk, actually.". Buehring, a professor of virology in the School of Public Health, has a reason to be wary of HeLa. Both her PhD and postdoc careers were spent working at UC Berkeleys Cell Culture Laboratory housed in the Naval Biosciences Laboratory in Oakland, a cell repository funded by the federal government that characterized and maintained cell lines for research scientists. She happened to be working there at a time when Dr. Walter Nelson-Rees, the co-director, was working hard to expose HeLas misdeeds. The vigorous cells crime? The contamination of other, less hardy cell lines.. Nelson-Rees was not the first to suspect contamination by HeLa cells. In the 1960s, Dr. Stanley Gartler, a research geneticist, released the initial "HeLa bomb". Gartler had discovered that the 18 different cell ...
No proteins after HeLa cell lysis - posted in Cell Biology: Hello, Ive been having a weird problem with my cell lysis lately. I lyse my cells, I quantify my proteins and load around 50ug sample on 8% gel and after transfer I stain my nitrocellulose membrane with Ponceau.... I dont see any bands on my membrane and I reveal the same membrane and see no band... to elaborate, I use the following lysis buffer Tris - 50mM NaCl - 100mM EDTA - 5mM NP40 - 1% I used regular HeLa...
In the present study we showed that suppression of the SBDS gene by shRNA caused a pronounced decrease in cell growth and an increase in apoptosis. The reduced cell growth and the accelerated apoptosis in SBDS-deficient cells were mediated predominantly through the Fas pathway, since blocking the Fas-signaling pathway at the Fas and caspase 8 levels significantly improved the defective cell growth phenotype of the SBDS-knockdown HeLa cells to levels close to those of control cells. Furthermore, SBDS-knockdown cells were hypersensitive to Fas stimulation and over-expressed Fas on their surface. In contrast to Fas, the expression ratios of the pro-apoptosis protein, Bax, to the anti-apoptosis proteins, BCL-2 and BCL-XL in the SBDS-deficient cells were not in favor of apoptosis, and inhibition of this pathway by a caspase 9 inhibitor did not improve cell growth. Since the current data are in agreement with those on primary SDS cells, and since the scrambled RNA control HeLa cells had similar ...
Human IFITM1 Knockout HeLa Cell Lysate parental control lysate. CRISPR/Cas9 generated negative control for antibody validation in WB.
The assembly of a rotating HeLa cell/gold rod aggregate at an acoustic nodal line in the xy plane. The video was taken under 500X overall magnification except for 00:23 - 00:32 and 01:16 - 01:42, where a 200X overall magnification was used.
Could anyone tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment.Thanks a lot!. ...
Page contains details about gold nanoparticle-loaded HeLa cells . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
HeLa S3 is a clonal derivative of the parent HeLa line (see ATCC CCL-2). S3 was cloned in 1955 by T.T. Puck, P.I. Marcus, and S.J. Cieciura.
Henriettas cells were the first immortal human cells ever grown in culture. They were essential to developing the polio vaccine. Many scientific landmarks since then have used her cells, including cloning, gene mapping and in vitro fertilization. Continue reading →. ...
A new study uses blood samples from pregnant women to track changes in the immune system leading up to birth, and predicts gestational age from the mothers immune signatures.. 0 Comments. ...
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The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cytotoxicity against HeLa cellscompared to the free ICD-85. The IC50 value at 72 h was reduced from 25 ± 2.9 μg/mL for free ICD-85 to 15.5 ± 2.4 μg/mL for ICD-85 NPs. However, LDH assay demonstrated that ICD-85 has dose-dependent cytotoxicity on HeLa cells while ICD-85 NPs exhibited weaker cytotoxicity on same cells. The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. Moreover, caspase-8 assay analysis demonstrated that the ICD-85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. Our results demonstrated that the
Epithelial-to-mesenchymal transition (EMT) renders cervical cancer cells more susceptible to metastasis, and reduced expression levels of E-cadherin have been shown in this malignancy (9). E-cadherin is a pivotal molecule involved in epithelial cell adhesion, aberrant expression of which has been demonstrated in various human malignancies as a result of irreversible (genetic) and reversible (epigenetic) modifications of the E-cadherin gene (CDH1), such that the epigenetic ones have been more implicated in E-cadherin downregulation (10).. In this study, we evaluated the impact of oxamflatin on HeLa cell line by determining the expression level of E-cadherin. Our results demonstrated that oxamflatin treatment leads to a time- and concentration-dependent increase in E-cadherin expression in cervical cancer cell lines.. There is ample evidence reporting that HDACIs could be applied to re-express silenced genes (9, 11). For example, valproic acid (VPA), as a HDAC inhibitor, led to the re-expression ...
371 Background: Fusion between malignant and primary, normal human cells results in the suppression of malignancy as measured by tumor formation of cells when inoculated into nude mice. Here we focus on hybrid cells after fusion of tumorigenic HPV18-positive HeLa cells and normal human fibroblasts. From these suppressed non-tumorigenic hybrid cells, rare tumorigenic segregant subpopulations can arise which have regained their tumorigenic capacity in vivo. Loss of a so far unidentified tumor suppressor gene(s) located on chromosome 11q13 plays a central role in this process. Aim of study: Analysis of in vitro and in vivo growth properties, differential chromosomal aberrations and differential gene expression patterns of non-tumorigenic hybrid cells compared to tumorigenic segregants and tumorigenic parental HeLa cells by cDNA microarray analysis. Materials and Methods: Differential gene expression patterns were analysed in non-tumorigenic and tumorigenic hybrid cells, parental HeLa cells and ...
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a marked stimulation of inorganic [32P]orthophosphate incorporation into HeLa-cell phosphatidylcholine (PC), phosphatidylethanolamine (PE), and lysophosphatidylethanolamine. The increased incorporation of inorganic [32P]orthophosphate into PE and lysophosphatidylethanolamine in the presence of TPA was not associated with an increase in PE synthesis as detected by the incorporation of [3H]serine or [3H]ethanolamine. The PC-specific exchange protein from beef liver was used to insert PC labeled with [3H]choline, inorganic [32P]orthophosphate, or [14C]arachidonic acid plus [3H]palmitic acid into the outer monolayer of intact HeLa cell membranes. Radioactivity from the latter two compounds was rapidly incorporated into PE and lysophosphatidylethanolamine; the incorporation was stimulated by TPA. It was concluded that TPA stimulated the formation of PE by base exchange between ethanolamine and PC.. ...
This is too long for a comment, so I have to write it in here:. Mostly this is because other cell lines are more practical in the lab.. Stem cells are much more tricky to maintain - especially if you want to keep their stem cell properties. They only grow very slow, tend to differentiate when they get too much stress, cellular signals and so on and need special media (these need to be defined, so that they are not containing factors which promote differentiation for example). For everyday experiments (like expression profiles, experiments on the effects of certain substances on the growth, gene expression or whatever of cells) "normal" cell lines are much more suited as these are much easier to keep in the lab using standard media. Additionally,experiments can be more easily repeated and reproduced, since these cells should behave always the same.. ...
en] Involvement of the AP-1 (activator protein-1) transcription factor has been demonstrated previously in the regulation of cell proliferation and cell-cycle progression, in the control of cell migration, invasion and metastasis, and in signal transduction, stress responsiveness, DNA replication and DNA repair. YB-1 (Y-box-binding protein-1) has also been implicated in many of these processes. However, the mechanism by which YB-1 mediates these processes is poorly understood. In the present study, we report that overexpression of a transfected gene encoding YB-1 in human HeLa cervical carcinoma cells significantly represses the transactivation of a minimal AP-1 reporter construct in response to the tumour promoter PMA. YB-1 also represses mRNA expression and PMA-induced promoter transactivation of the endogenous AP-1 target gene encoding matrix metalloproteinase-12 (metalloelastase). YB-1 transrepression of both the minimal and matrix metalloproteinase-12 promoter reporter constructs is ...
One of the characteristics of picornavirus infection of cells in tissue culture is a specific inhibition of utilization of host cell mRNA for protein synthesis. In this study we show that human rhinovirus 14 is similar to poliovirus in that the inhib
Twenty years ago this morning, I had to defend a body of work that contained this paragraph on page 24: HeLa cells are a human cervical carcinoma cell line having a doubling time of 24 hr and were obtained from Dr. Bert Flanegan, Dept. of Immunology and Microbiology, University of Florida. HeLa cells were maintained…. ...
The cationic complexes (1,2-diaminoethane)(maltolato)platinum(II) ([Pt(en)(ma)]+) and (1R,2R-1,2-diaminocyclohexane)(maltolato)platinum(II) ([Pt(R,R-DACH)(ma)]+) have been prepared and the structure of [Pt(R,R-DACH)(ma)]NO3 has been determined by single crystal X-ray diffraction. The geometry of the metal in [Pt(R,R-DACH)(ma)]NO3 is essentially square planar and the maltolate ligand has a geometry similar to other chelate complexes involving this ligand. The cytotoxicities of the compounds have been assessed in the human cell lines HeLa and K562 and the IC50 values are approximately 32 microM in HeLa cells and 26 microM in K562 cells. In these cell lines the cytotoxicity of cisplatin is higher than the maltolate complexes by a factor of 2 to 3 whereas the cytotoxicity of carboplatin is lower than the maltolate complexes ...
HeLa whole cell lysate (Positive control), 0.1 mg. This whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.
AT cells are extremely sensitive to ionizing radiation. Since the AT gene has homology to phosphatidylinositol 3 kinases (PI 3-kinases), wortmannin, a specific inhibitor of PI 3-kinase, was used to determine if PI 3-kinase activity regulates radiation sensitivity. Human and murine cells exposed to wortmannin alone did not display significant cytotoxicity. Wortmannin in combination with radiation was an effective radiosensitizer of murine NIH-3T3 fibroblasts, with a sensitizer enhancement ratio of 1.8 at 10% survival, and had a similar effect on the human tumor cell lines HeLa, SW480, and MCF-7. Wortmannin inhibited the induction of p53 DNA-binding activity by actinomycin D and radiation and blocked the transcriptional activation of a p53 CAT reporter gene by actinomycin D. Wortmannin radiosensitized both wild-type (NIH-3T3 and MCF-7) and mutant (SW480 and HeLa) p53 cells, indicating that p53 induction was not required for radiosensitization by worthmannin. The results suggest that a ...
The Mab K1 was generated by immunization of mice with the human ovarian carcinoma cell line OVCAR-3 (3) . The reactivity of Mab K1 against a variety of different human tumor cell lines was tested using immunofluorescence (3) . It showed reactivity with several ovarian cancer cell lines, including OVCAR-2, OVCAR-3, OVCAR-5, A-1847, and SKOV3; cervical cancer cell lines HeLa and KB; and gastric cell lines AGS and HTB103. No reactivity was detected with breast, colon, or prostate cancer cell lines. Care must be taken with the use of Mab K1 because its reactivity is acid labile (5) .. The reactivity of Mab K1 against normal human tissues was tested by immunohistochemistry using cryostat tissue sections (3) . Most normal tissues showed no reactivity with Mab K1, with the exception of mesothelial cells that line the peritoneal, pleural, and pericardial cavities. There was also weak reactivity with the basal cells of the trachea and cells in the Fallopian tubes. A similar immunoreactivity was seen in ...
We have been using HeLa cells in my laboratory since 1982, when I arrived at Columbia University Medical Center fresh from postdoctoral work with David Baltimore at MIT. I brought with me a line of HeLa cells and used them for 30 years for our research on viruses. Here is a story of how we lost the cells and then found them ten months later.. As everyone knows, the continuous HeLa cell line was derived from a cervical tumor taken from Henrietta Lacks in 1951 (if you dont know the story, you should read Rebecca Skloots The Immortal Life of Henrietta Lacks, or my shorter summary). When I arrived at the Baltimore lab in 1979, they were using cells derived from the S3 clone of HeLa cells that had been produced by Philip Marcus in the 1950s. I write derived from because someone at MIT had further cloned the S3 line and selected one that was particularly susceptible and permissive to poliovirus infection. This was the cell line that I took with me to Columbia in 1982.. Because we use so many HeLa ...
Dr. Karen Resendes, Westminster College assistant professor of biology, recently published two articles on HeLa cell research, one of which was in collaboration with Gretchen Diffendall 14.
An attempt was made to induce calciphylaxis and direct calcifying reaction according to Selye2in HeLa cell cultures. With our experimental conditions, we obtained neither a calciphylaxis nor a...
Derived from Henrietta Lacks in 1951, HeLa is an immortal cell line that is useful for its remarkable prolificity and durability. It was the inspiration for the national bestseller, The Immortal Life of Henrietta Lacks, written by the incredibly attractive Rebecca Skloots. Because of their high reproductive rate, HeLa cells are a good beginner transfection cell line to test the efficacy of the CPNP delivery of the origami. ...
Derived from Henrietta Lacks in 1951, HeLa is an immortal cell line that is useful for its remarkable prolificity and durability. It was the inspiration for the national bestseller, The Immortal Life of Henrietta Lacks, written by the incredibly attractive Rebecca Skloots. Because of their high reproductive rate, HeLa cells are a good beginner transfection cell line to test the efficacy of the CPNP delivery of the origami. ...
The final argument Ill advance against HeLa in cancer research is the fact that it has been in culture so long. HeLa should be seen as something like a bizarre new species found only in laboratories, not a representative of human cancer. All that time in culture has certainly enabled HeLa (or all the different HeLa) to evolve far away from the tumor which killed Henrietta Lacks. Again, this is a flaw in all in vitro propagated cell line studies, but given that HeLa is the oldest it is likely the most affected (granted, what wed really like to know is actual time in culture). Patient-derived xenografts are far more likely to be informative of tumors, but their expense and newness has meant they havent yet fully displaced in vitro lines from research programs ...
The final argument Ill advance against HeLa in cancer research is the fact that it has been in culture so long. HeLa should be seen as something like a bizarre new species found only in laboratories, not a representative of human cancer. All that time in culture has certainly enabled HeLa (or all the different HeLa) to evolve far away from the tumor which killed Henrietta Lacks. Again, this is a flaw in all in vitro propagated cell line studies, but given that HeLa is the oldest it is likely the most affected (granted, what wed really like to know is actual time in culture). Patient-derived xenografts are far more likely to be informative of tumors, but their expense and newness has meant they havent yet fully displaced in vitro lines from research programs ...
FIG. 2. PP2A mediates RPA32 dephosphorylation at T21 and S33. (A) HeLa cells were transfected with a mock siRNA oligonucleotide or with oligonucleotides against the catalytic subunits of PP1, PP2A, PP4, PP5, and PP6. Twenty-four hours posttransfection, cells were pulse treated with HU (0.2 mM, 24 h), and RPA32 dephosphorylation at T21/S33 was compared at the indicated time points. (B) HeLa cells transfected with control siRNA or siRNA against PP2A/C were pulse treated with HU (0.2 mM, 24 h). At 0 to 12 h postrelease, the cells were fixed and costained with DAPI and anti-RPA32pS33 and anti-γ-H2AX antibodies. The DNA damage-inducible foci were visualized by immunofluorescence microscopy, and the percentage of phospho-RPA32 and γ-H2AX focus-positive cells was determined, normalized, and plotted. The error bars represent the standard deviations from three independent experiments. (C) Immunoprecipitation (IP) assays were performed in unperturbed cells or cells recovering from exposure to pulses of ...
This section illustrates a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye, Dil, which targets membranes. The absorption maximum of Dil is 549 nanometers and the emission maximum occurs at 565 nanometers (in the yellow region of the visible light spectrum). Fluorescence intensity arising from labeled cytoskeletal membrane components is quite evident in the image even through the probe emission peak is 30 nanometers below the Y-2E/C filter set dichromatic mirror cut-on wavelength.
Get an answer for Why has the discovery of the existence of HeLa cells been so difficult for the Lacks family? Do you think that her childrens lives would have turned out significantly different had Henrietta not died so young? and find homework help for other Science questions at eNotes
hi i,m rodrigo from chile and i have the same question of which proteins can be secreted by HeLa cells into the growth medium, if you know the answer please send to me a this mail rodrigosakuragi from gmail.com ...
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
Netrin-1 is a secreted protein that was first identified 20 years ago as an axon guidance molecule that regulates midline crossing in the CNS. It plays critical roles in various tissues throughout development and is implicated in tumorigenesis and inflammation in adulthood. Despite extensive studies, no inherited human disease has been directly associated with mutations in NTN1, the gene coding for netrin-1. Here, we have identified 3 mutations in exon 7 of NTN1 in 2 unrelated families and 1 sporadic case with isolated congenital mirror movements (CMM), a disorder characterized by involuntary movements of one hand that mirror intentional movements of the opposite hand. Given the diverse roles of netrin-1, the absence of manifestations other than CMM in NTN1 mutation carriers was unexpected. Using multimodal approaches, we discovered that the anatomy of the corticospinal tract (CST) is abnormal in patients with NTN1-mutant CMM. When expressed in HEK293 or stable HeLa cells, the 3 mutated netrin-1 ...
DataMed is a prototype biomedical data search engine. Its goal is to discover data sets across data repositories or data aggregators. In the future it will allow searching outside these boundaries. DataMed supports the NIH-endorsed FAIR principles of Findability, Accessibility, Interoperability and Reusability of datasets with current functionality assisting in finding datasets and providing access information about them.
Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells--taken without her knowledge--became one of the most important tools in medicine. The first "immortal" human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. If you could pile all HeLa cells ever grown onto a scale, theyd weigh more than 50 million metric tons--as much as a hundred Empire State Buildings. HeLa cells were vital for developing the polio vaccine; uncovered secrets of cancer, viruses, and the atom bombs effects; helped lead to important advances like in vitro fertilization, cloning, and gene mapping; and have been bought and sold by the billions ...
comes out next month, written about the woman and the cells which should have made her famous. Rebecca Skloot been researching it for something like 10 years and its got some great reviews. Im going to pre-order a copy, and if youd like to know more about HeLa cells and Henrietta Lacks, do the same! Its a story that deserves to be heard, and if there are enough pre-orders, Amazon will help promote the book. Plus its 30% off at the moment. What more could you want? Heres the blurb.. Her name was Henrietta Lacks, but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells-taken without her knowledge-became one of the most important tools in medicine. The first "immortal" human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. If you could pile all HeLa cells ever grown onto a scale, theyd weigh more than 50 million metric tons-as much as a hundred Empire State ...
Figure 1: Effects of PKR on the proliferation and translation. (a) Effects of PKR on the proliferation of HeLa cells. After being transfected with plasmids PKR, PKR siRNA, or GFP, HeLa cells were plated in multiple wells of a 96-well plate and grown for 24 hr for cell proliferation assays. Cells from the sample preparations were collected for immunoblotting. Proliferation rate of the control sample was normalized to 100%. PKR, WT PKR; si-PKR, PKR siRNA; Ctrl, GFP. Upper panel, averaged data (N=4 ...
Lysates of HeLa cells treated with UV or untreated cells for use as controls in western blotting to analyze UV-induced DNA damage and/or apoptosis.
When we work with cell lines in the lab, we often work with HeLa cells. They can live in a vial of nutrients, and from a small sample you can grow a large
Within this site you can view figures from the papers, supplementary data, and interactively explore the cell cycle dataset. In addition, raw data and processed data are available for download.. ...
AccuTarget™ Validated Real-Time PCR Primer Library for Human is designed by Bioneers bioinformatics tool and targeting for human genome. cDNA was synthesized using Human PTGS2 target primer of those and Human total RNA identically quantified from Hela cell and blood cell with AccuPower® CycleScript RT PreMix(K-2044, Bioneer). Gene analysis was carried out both Hela cell and blood cell by operating Real-time PCR reaction(95°C 10 min, 1 cycle and 95°C 10 sec, 58°C 25 sec, 72°C 30 sec, 41 cycles) using the cDNA, AccuPower® 2X Greenstar qPCR Master Mix and Exicycler™ 96 Real-Time Thermal Block(Cat. No. A-2060).. ...
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The SMURF2 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The L3MBTL2 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
The research world’s most famous human cell has had its genome decoded, and it’s a mess. German researchers this week report the genome sequence of the HeLa cell line, which originates from a deadly cervical tumor taken from a patient named Henrietta Lacks (Slashdot has previously noted ...
News & Opinion Telomere Length and Childhood Stress Dont Always Correlate Shorter telomere length is widely considered a manifestation of stress in young children, but the results of a new study find its more complicated than that. ...
One of the first things a biomedical researcher learns is that its very hard to grow most human cells in the lab for an extended period. In fact, once removed from the human body, most cells will either die immediately or reproduce only a limited number of times. Thats why it was so significant in…
She was poor, black and terminally ill with cervical cancer. Henrietta Lacks was a 31-year-old mother of five when she died in 1951, but her name and memory live on in the form of a remarkable lineage of continually dividing cells that have achieved, to all intents and purposes,
of palladium(II) ions and the corresponding ligands in different relations of concentration, and Pd-L-A system in order to investigate the effect of binary complexes [PdL] to nucleosides entering in DNA. The fluorescent and absorption spectroscopy were used to see interactions of the synthesized compounds and HSA and DNA molecules. The results showed that there is a good binding of our system with biologically significant molecules, which led us to the next phase, involving the in vitro experiments. The in vitro biological activity of these compounds was examined by the MTT cytotoxicity assay and methods for detecting apoptosis. Experimental results correspond with theoretical and indicate that the biological activity of the palladium(II) compounds is highly dependent on the structure of the nitrogen-oxygen donor ligands. HeLa cell line has been shown the highest sensitivity, especially in the case of K2[Pd(mdp)] · H2O and H4obbz compounds. Recent studies have shown that some palladium(II) ...
Typical parameter values for a bacterial E. coli cell, the single-celled eukaryote S. cerevisiae (budding yeast), and a mammalian HeLa cell line. Note that these are crude characteristic values for happily dividing cells of the common lab strains. Adapted with changes from U. Alon, "Introduction to Systems Biology", CRC Press 2006 ...
HeLa nuclear extract lysate Lysates datasheet (ab150036). Abcam offers quality products including antibodies, assays and other reagents.
Still, it would be another six months before anyone but Nelson-Rees, Akeson, Cailleau, and Bailar had any inkling that MAC-21 and 2563 were not what they were advertised to be. Scientists who had read Akesons report on lung tumor-specific antigens would have had an entire year to follow his lead without any indication it might be a dead end. Finally Nelson-Reess letter appeared in the journals June 1978 issue. It was followed by a letter from Akeson who said that based on Nelson-Reess work it seems reasonable to presume the cells he had thought were from a lung tumor were actually HeLa cells. He added, however, that some of the antigens in these cells did seem to resemble those seen in genuine lung tissue, concluding that further studies were needed to sort through the confusion. Following Akesons letter was a one-paragraph statement from Cailleau conceding that the single culture of MAC-21 she had checked was HeLa. I am certain that the original MAC-21 was obtained from a mucoid ...
This is the complete description of the cell line that you selected. By clicking on a generic term (e.g., SPECIES) you will go to the corresponding index (e.g., index of species), while by clicking on a specific term (e.g., retinoblastoma) you will go to the list of corresponding cell lines (e.g., retionoblastoma cell lines ...
Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.. ...
Annotation: The results show that Myt1-mediated suppression of Cdc2 activity is not indispensable for the regulation of a broad range of mitotic events but is specifically required for the control of intracellular membrane dynamics during mitosis in Hela cells.Imported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/manual/evidences#ECO:0000313">More…,/a>,/p> Automatic assertion inferred from database entriesi. ...
PLK2 Antibody 15956-1-AP has been identified with ELISA, WB, IP. 15956-1-AP detected 75-80 kDa band in HeLa cells with 1:500-1:1000 dilution...
Cell, Cells, Ligands, Memory, Role, Survival, T Cells, Bfl, Cell Lines, Family, Hela Cells, Adaptive Immune Response, Concentration, Infection, Innate Immune Response, Mammals, Plays, Tissue, Autoimmune Diseases, B Cells
KLF5 Antibody 21017-1-AP has been identified with ELISA, IF, IHC, IP, WB. 21017-1-AP detected 51-55 kDa band in HeLa cells with 1:500-1:2000 dilution...
AKT Antibody 60203-2-Ig has been identified with ELISA, IF, IHC, IP, WB. 60203-2-Ig detected 62 kDa band in HeLa cells with 1:2000-1:10000 dilution...
Background The Securinega-type alkaloids occur in plants belonging to Euphorbiaceae family. One of the most widely distributed alkaloid of this group is securinine, which was identified next to allosecurinine in Phyllanthus glaucus (leafflower). Recently, some Securinega-type alkaloids have paid attention to its antiproliferative potency towards different cancer cells. However, the cytotoxic properties of allosecurinine have not yet been evaluated. Methods The cytotoxicity of the extract, alkaloid fraction obtained from P. glaucus, isolated securinine and allosecurinine against HeLa cells was evaluated by real-time xCELLigence system and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was detected by annexin V and 7-amino-actinomycin (7-AAD) staining and confirmed with fluorescent Hoechst 33342 dye. The assessment of mitochondrial membrane potential (MMP), reactive oxygen species (ROS) generation, the level of extracellular signal-regulated protein kinases 1 and 2
Objective: Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of Kelussia odoratissima (K. odoratissima) extract associated with radiotherapy in cervical cancer cells (HeLa cell line).Materials and Methods: Different concentration of the extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min)-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.Result: K. odoratissima decreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control,K. odoratissima induced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved in K. odoratissima-induced toxicity. It was also shown that K. odoratissima sensitizes cells to radiation-induced toxicity.Conclusion: Our result showed
Abstract: Cancer is one of the leading causes of death worldwide, therefore struggles to find more effective treatment and prevention is needed. Several studies has been performed using natural ingredients, one of which is Temu Kunci (B. pandurata). Temu Kunci extract contains flavonoid pinostrobin that has been showed as having cytotoxicity effects. Cytotoxicity tests of pinostrobin have been performed on several tumor cell lines, but its cytotoxicity effect on HeLa cell line has never been reported. ...
Mammalian cell invasion assays, using metacyclic trypomastigotes of Trypanosoma cruzi G and CL strains, showed that the CL strain enters target cells in several-fold higher numbers as compared with the G strain. Analysis of expression of surface glycoproteins in metacyclic forms of the two strains by iodination, immunoprecipitation and FACS, revealed that gp90, undetectable in the CL strain, is one of the major surface molecules in the G strain, that expression of gp82 is comparable in both strains and that gp35/50 is expressed at lower levels in the CL strain. Purified gp90 and gp35/50 bound more efficiently than gp82 to cultured HeLa cells. However, the intensity of the Ca2+ response triggered in HeLa cells by gp82 was significantly higher than that induced by gp35/50 or gp90. Most of the Ca2+ signalling activity of the metacyclic extract towards HeLa cells was due to gp82 and was inhibitable by gp82-specific monoclonal antibody 3F6. Ca2+ mobilization was also triggered in metacyclic ...
We propose the collinear balanced detection (CBD) technique for noise suppression in fiber laser (FL)-based stimulated Raman scattering (SRS) microscopy. This technique reduces the effect of laser intensity noise at a specific frequency by means of pulse splitting and recombination with a time delay difference. We experimentally confirm that CBD can suppress the intensity noise of second harmonic (SH) of Er-FL pulses by 13dB.The measured noise level including the thermal noise is higher by only ~1.4 dB than the shot noise limit. To demonstrate SRS imaging, we use 4-ps SH pulses and 3-ps Yb-FL pulses, which are synchronized subharmonically with a jitter of 227 fs. The effectiveness of the CBD technique is confirmed through SRS imaging of a cultured HeLa cell.. ©2012 Optical Society of America. Full Article , PDF Article ...
The effect of gadolinium chloride (Gd) on the proliferation of HeLa cells was investigated at lower concentration. The results obtained by MTT and cell cycle analysis showed that Gd promoted proliferation by inducing S phase entry in HeLa cells at th
Li J.J., Kelly T.J.. We recently described a soluble cell-free system derived from monkey cells that is capable of replicating exogenous plasmid DNA molecules containing the simian virus 40 (SV40) origin of replication (J.J. Li, and T.J. Kelly, Proc. Natl. Acad. Sci. U.S.A. 81:6973-6977, 1984). Replication in the system is completely dependent upon the addition of the SV40 large T antigen. In this report we describe additional properties of the in vitro replication reaction. Extracts prepared from cells of several nonsimian species were tested for the ability to support origin-dependent replication in the presence of T antigen. The activities of extracts derived from human cell lines HeLa and 293 were approximately the same as those of monkey cell extracts. Chinese hamster ovary cell extracts also supported SV40 DNA replication in vitro, but the extent of replication was approximately 1% of that observed with human or monkey cell extracts. No replication activity was detectable in extracts ...
Figure 2. Quercetin-3-o-glucosida and Sianidin-3-o-glukosida. 6. Mechanism of Anticancer Research. Quercetin and anthocyanin is a substance found in many plants, especially mulberry (Morus alba L.) which has potential as a chemopreventive agent. Anthosianin type that has an effect as a chemopreventive agent is sianidin-3-O-glukosida. Secara in vitro, sianidin-3-O-glukosida known to reduce the invasion of lung cancer cells A549 and can reduce cell motility (Chen et al., 2006) and quercetin is known to inhibit the growth of HL-60 cells were significan and can induce differentiation of HL-60 cells expressing antigen 66B CD and CD 14 (Kim et al., 2000). Quersetin also known to inhibit the growth, adhesion and migration of HeLa cells and is able to trigger apoptosis in HeLa cell cultures. At a dose of 20-80 micromol / l quersetin, the inhibition of adhesion, migration and invasion of HeLa cells increased 37-83% (Zhang, 2008). In addition, quercetin is also known to enhance the inhibitory effect of ...
Dioscin, a natural product, has activity against glioblastoma multiforme, lung cancer and colon cancer. In this study, the effects of dioscin against human cervical carcinoma HeLa and SiHa cells were further confirmed, and the possible mechanism(s) were investigated. A transmission electron microscopy (TEM) assay and DAPI staining were used to detect the cellular morphology. Flow cytometry was used to assay cell apoptosis, ROS and Ca2+ levels. Single cell gel electrophoresis and immunofluorescence assays were used to test DNA damage and cytochrome C release. The results showed that dioscin significantly inhibited cell proliferation and caused DNA damage in HeLa and SiHa cells. The mechanistic investigation showed that dioscin caused the release of cytochrome C from mitochondria into the cytosol. In addition, dioscin significantly up-regulated the protein levels of Bak, Bax, Bid, p53, caspase-3, caspase-9, and down-regulated the protein levels of Bcl-2 and Bcl-xl. Our work thus demonstrated that dioscin
Yersinia pseudotuberculosis YopB and YopD proteins are essential for translocation of Yop effector proteins into the target cell cytosol. YopB is suggested to mediate pore formation in the target cell plasma membrane, allowing translocation of Yop effector proteins, although the function of YopD is unclear. To investigate the role in translocation for YopD, a mutant strain in Y. pseudotuberculosis was constructed containing an in frame deletion of essentially the entire yopD gene. As shown recently for the Y. pestis YopD protein, we found that the in vitro low calcium response controlling virulence gene expression was negatively regulated by YopD. This yopD null mutant (YPIII/pIB621) was also non-cytotoxic towards HeLa cell monolayers, supporting the role for YopD in the translocation process. Although other constituents of the Yersinia translocase apparatus (YopB, YopK and YopN) are not translocated into the host cell cytosol, fractionation of infected HeLa cells allowed us to identify the ...
Primer recognition proteins (PRP) are cofactors for DNA polymerase alpha and may have a role in lagging-strand DNA replication. PRP is composed of two subunits, which we have previously identified as the protein-tyrosine kinase substrate annexin II and phosphoglycerate kinase (PGK). In this study, we have examined the physiological involvement of these proteins in DNA synthesis and cell proliferation. When exponentially growing human HeLa cells are exposed to antisense phosphorothioate oligodeoxynucleotides to annexin II, ongoing DNA synthesis is reduced. The extent of reduction with antisense oligodeoxynucleotide to PGK was much less than with the antisense annexin II oligodeoxynucleotide. Reductions in the labeling and mitotic indices of HeLa cell cultures are seen after exposure to antisense oligodeoxynucleotides. Flow cytometric analyses indicate that progression from S phase to G2 phase of the cycle is retarded by exposure of cells to the antisense oligodeoxynucleotides. Corresponding sense ...
The effects of addition of 1 microM-dexamethasone on the rate of transfer of mannose from GDP-mannose into mannolipid have been studied in HeLa cell cultures. Concurrent with an increase in incorporation of mannose into glycoproteins, the incorporation of mannose from GDP-mannose in vitro into mannolipid and dolichol-linked oligosaccharides was increased after dexamethasone treatment. Stimulation of mannolipid synthesis showed a correlation with the 11 beta, 17 alpha, 21-trihydroxy structure of C21 steroids. Dexamethasone treatment also resulted in an increased incorporation of acetate into dolichol and dolichyl phosphate. The results suggest that the effect of dexamethasone on the cell-surface glycoprotein accumulation is related to increased sugar-linked dolichol synthesis. ...
We are using the BPV E2 protein to characterize the status of cell cycle regulatory pathways in human cervical carcinoma cell lines and to identify cellular components that regulate proliferation of these cells. Our results indicate that the E2 protein can mobilize dormant growth-regulatory programs in HPV-positive cervical carcinoma cells, resulting in growth inhibition. Presumably, these malignant cells can tolerate the existence of these growth-inhibitory programs because they are normally neutralized by expression of the endogenous HPV E6 and E7 proteins. HT-3 cells contain HPV30 DNA, and the E2 protein represses HPV E6/E7 expression in these cells, as it does in HPV18-containing HeLa cells. Therefore, the growth-inhibitory effects of the E2 protein in cervical carcinoma cell lines may be solely a consequence of E6/E7 repression. Although we have not carried out an exhaustive survey of various cell lines, we have not identified HPV-negative cells that are growth-inhibited by infection with ...
Levels of the elF-2a-specific protein kinase, PkR, are higher in several breast carcinoma cell lines including the estrogen-responsive cell lines, MOF-7 and T-47D, as well as estrogen- independent cell lines, BT-20 and MDA-MB-468, compared with the normal breast cell line, Hs578 Bst, or the human HeLa cell line. In contrast, the phosphorylation state of elF-2a is very low in the breast carcinoma cell lines compared to the normal human breast cell line Hs578 Bst and HeLa cells, even at high cell densities, suggesting an inhibition of PkR activity in the breast oarninoma cell lines. In support of this hypothesis, treatment of cells with either alpha- or Beta-interferon, although increasing PKR levels slightly, do not result in higher steady state levels of elF-2a phosphorylation. These results suggest that deregulation of PKR activity is occurring at some level in breast carcinoma cell lines. This deregulation could result from increased levels/activities of cellular PKR inhibitory proteins, or from
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a promising candidate for the treatment of cancer, because it preferentially induces apoptosis in numerous cancer cells with little or no effect on normal cells. 5,7-Dihydroxyflavone is a dietary flavonoid commonly found in many plants. Here we show that the combined treatment with 5,7-dihydroxyflavone and TRAIL at subtoxic concentrations induced strong apoptotic response in human hepatocarcinoma HepG2 cells, acute leukemia Jurkat T cells, and cervical carcinoma HeLa cells. We further investigated the mechanisms by which 5,7-dihydroxyflavone augments TRAIL-induced apoptosis in HepG2 cells. 5,7-Dihydroxyflavone up-regulated the expression of pro-apoptotic protein Bax, attenuated the expression of anti-apoptotic proteins Bcl-2, Mcl-1, and IAPs, and reduced the phosphorylation levels of Akt and STAT3, weakening the anti-apoptotic signals thus facilitating the process of apoptosis. Moreover, 5,7-dihydroxyflavone and TRAIL were well tolerated

Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells | Journal of Cell ScienceSite of transcription of ribosomal RNA and intranucleolar structure in HeLa cells | Journal of Cell Science

Sites of transcription of ribosomal RNA in HeLa cells were visualized by electron microscopy. Cells were either incubated with ... Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells ... Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells ... Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells ...
more infohttp://jcs.biologists.org/content/107/2/639

Hypoxia stimulates invasion and migration of human cervical cancer cell lines HeLa/SiHa through the Rab11 trafficking of...Hypoxia stimulates invasion and migration of human cervical cancer cell lines HeLa/SiHa through the Rab11 trafficking of...

Our results showed that in cervical cancer cell... lines HeLa/SiHa, Rac1 activation in hypoxia could stimulate invasion and ... Our results showed that in cervical cancer cell... lines HeLa/SiHa, Rac1 activation in hypoxia could stimulate invasion and ... Hypoxia stimulates invasion and migration of human cervical cancer cell lines HeLa/SiHa through the Rab11 trafficking of ... could stimulate invasion and migration of cervical cancer cell lines HeLa/SiHa in hypoxia. Activation of Rac1 activity by ...
more infohttp://d.scholar.cnki.net/detail/SSJDTEMP_U/SSJD11B5D09FC5D6C28129E1A6F9CE8167D1

How HeLa Cells Works | HowStuffWorksHow HeLa Cells Works | HowStuffWorks

Learn about the discovery and ethics of HeLa cells. ... HeLa cells have been star players in medical research for ... Under the right conditions, HeLa cells form an immortal cell line; they divide indefinitely. Remember that HeLa cells were ... But whats a HeLa cell? Its a line, or population, of cells, taken from a person and used in scientific research. Cell lines ... HeLa cells have even been sent to outer space, proving that cancer cells can grow there. Almost since its creation, the HeLa ...
more infohttps://science.howstuffworks.com/life/cellular-microscopic/hela-cell.htm/printable

HeLa Cells with Dil | MicroscopyUHeLa Cells with Dil | MicroscopyU

This section illustrates a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye, Dil, which ... Fluorescence emission intensity from a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye ... HeLa Cells with Dil. Y-2E/C Bandpass Emission (Medium Bandwidth Excitation) Yellow Set. ...
more infohttps://www.microscopyu.com/gallery-images/hela-cells-with-dil

Cell-biology] Proteins secreted by HeLa cellsCell-biology] Proteins secreted by HeLa cells

... Rodrigo Mancilla via cellbiol%40net.bio.net (by rodrigosakuragi from gmail.com) ... hi i,m rodrigo from chile and i have the same question of which proteins can be secreted by HeLa cells into the growth medium, ... Previous message: [Cell-biology] antibodies, assays, antigens sources *Next message: [Cell-biology] Markers, detection and ... Previous message: [Cell-biology] antibodies, assays, antigens sources *Next message: [Cell-biology] Markers, detection and ...
more infohttp://www.bio.net/bionet/mm/cellbiol/2006-October/015838.html

Dividing HeLa cells,… | Wellcome CollectionDividing HeLa cells,… | Wellcome Collection

HeLa cells are an immortal human epithelial cell line derived from a cancerous tumour of the cervix (adenocarcinoma). It was ... HeLa cells have been used extensively around the world in many different fields of research including cancer research, ... The cell will finish dividing its nuclear material and then cytoplasm in order to physically separate into two daughter cells. ... Chromosomes in the cell nucleus (purple), microtubules in the cell cytoskeleton (tubulin; green), and actin (red) are visible ...
more infohttps://wellcomecollection.org/works/vjq5c26r?query=chromosomes+histones

Large-scale characterization of HeLa cell nuclear phosphoproteins | PNASLarge-scale characterization of HeLa cell nuclear phosphoproteins | PNAS

HeLa Cell Lysate and Synthetic Phosphopeptide Library. Cell lysate was prepared by using unsynchronized HeLa cells lysed by ... HeLa Cell Nuclear Preparation, Preparative SDS/PAGE Separation, and in-Gel Proteolysis. HeLa cell nuclear preparation was as ... Cell Signaling Technology, Beverly, MA) for synthetic peptides, and S. Gerber (Harvard Medical School) for HeLa cell lysate. ... Large-scale characterization of HeLa cell nuclear phosphoproteins. Sean A. Beausoleil, Mark Jedrychowski, Daniel Schwartz, ...
more infohttps://www.pnas.org/content/101/33/12130?ijkey=036008c9c6f2402ac240555de3621be50293cc30&keytype2=tf_ipsecsha

Large-scale characterization of HeLa cell nuclear phosphoproteins | PNASLarge-scale characterization of HeLa cell nuclear phosphoproteins | PNAS

HeLa Cell Lysate and Synthetic Phosphopeptide Library. Cell lysate was prepared by using unsynchronized HeLa cells lysed by ... HeLa Cell Nuclear Preparation, Preparative SDS/PAGE Separation, and in-Gel Proteolysis. HeLa cell nuclear preparation was as ... Cell Signaling Technology, Beverly, MA) for synthetic peptides, and S. Gerber (Harvard Medical School) for HeLa cell lysate. ... Large-scale characterization of HeLa cell nuclear phosphoproteins. Sean A. Beausoleil, Mark Jedrychowski, Daniel Schwartz, ...
more infohttps://www.pnas.org/content/101/33/12130?ijkey=fcd5588d211faff9daa9d66885a663b1dc88c717&keytype2=tf_ipsecsha

Human Cell Cycle : Hela Cells | HomeHuman Cell Cycle : Hela Cells | Home

Within this site you can view figures from the papers, supplementary data, and interactively explore the cell cycle dataset. In ... "Identification of Genes Periodically Expressed in the Human Cell Cycle and Their Expression in Tumors." ...
more infohttp://genome-www.stanford.edu/Human-CellCycle/Hela/

HeLa cell Archives - Popular MechanicsHeLa cell Archives - Popular Mechanics

Video of live HeLa cell: 3rd place, Small World in Motion competition. 29 April 2014 ...
more infohttp://www.popularmechanics.co.za/tag/hela-cell/

Mathematical modeling of the heat-shock response in HeLa cells.  - PubMed - NCBIMathematical modeling of the heat-shock response in HeLa cells. - PubMed - NCBI

Mathematical modeling of the heat-shock response in HeLa cells.. Scheff JD1, Stallings JD2, Reifman J3, Rakesh V1. ... Our model was also in agreement with experiments showing that the number of HSF molecules in a HeLa cell is roughly 100 times ... the literature to obtain a compendium of in vitro hyperthermia experiments investigating the heat-shock response in HeLa cells ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/26200855

RNA in HeLa cell - Cell BiologyRNA in HeLa cell - Cell Biology

Top : Forum Archives: : Cell Biology. RNA in HeLa cell - amount of RNA in a HeLa cell (Jun/08/2007 ). Hey all,. Could anyone ... From a T75 flask of subconfluent HeLa cells, I extracted about: 3ug/ul. I used the TRI Reagent.. Hope this helps.. L ... Could anyone tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment. ... tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment.Thanks a lot! ...
more infohttp://www.protocol-online.org/biology-forums/posts/27955.html

HeLa cells, SEM - Stock Image - C003/6008 - Science Photo LibraryHeLa cells, SEM - Stock Image - C003/6008 - Science Photo Library

HeLa cells are a continuously cultured cell line of human cancer cells, which are immortal and so thrive in the laboratory. ... HeLa cells, coloured scanning electron micrograph (SEM). HeLa cells are a continuously cultured cell line of human cancer cells ... The spherical cell at centre is undergoing apoptosis (programmed cell death). Apoptosis occurs when a cell becomes old or ... HeLa cells, coloured scanning electron micrograph (SEM). ...
more infohttps://www.sciencephoto.com/media/100234/view/hela-cells-sem

At The Cell Counter : HeLa Cells | Molecular DevicesAt The Cell Counter : HeLa Cells | Molecular Devices

HeLa cells is the oldest and most commonly used human cell line. ... HeLa cell is an immortal cell lines to be grown in the lab and ... HeLa cells were the first cell lines to be grown in the lab and are still used in countless biomedical research projects today ... HeLa cells were seeded at 1000 cells per well and allowed to grow overnight. Then they were treated with anisomycin (red ... HeLa cells were seeded at 4 initial densities in a 96-well plate: 250 (purple), 500 (blue), 1000 (green), and 2000 (red) cells ...
more infohttps://www.moleculardevices.com/cell-counter-hela-cells

Dividing Hela Cells, Sem Poster by Steve GschmeissnerDividing Hela Cells, Sem Poster by Steve Gschmeissner

... All posters are professionally printed, packaged, and shipped within 3 ... Dividing HeLa cells. Coloured scanning electron micrograph (SEM) of HeLa cells undergoing cytokinesis (cell division). ... HeLa cells are a continuously cultured cell line of human cancer cells, which are immortal and so thrive in the laboratory. ... photographs hela cell cancer cell culture dividing replicating biology genetics medicine oncology coloured sem scanning ...
more infohttps://fineartamerica.com/featured/3-dividing-hela-cells-sem-steve-gschmeissner.html?product=poster

Putative mechanisms of antitumor activity of cyano-substituted heteroaryles in HeLa cells | SpringerLinkPutative mechanisms of antitumor activity of cyano-substituted heteroaryles in HeLa cells | SpringerLink

... which do not bind to DNA but are highly cytotoxic against the human tumor cell line HeLa, were analyzed for their antitumor ... Cyano-substituted heteroaryles HeLa cells Random forest Apoptosis Cytoskeleton disruption Antioxidants This is a preview of ... Putative mechanisms of antitumor activity of cyano-substituted heteroaryles in HeLa cells. ... and apoptosis in HeLa cervical carcinoma cells. J Virol 77:1551-1163PubMedCrossRefGoogle Scholar ...
more infohttps://link.springer.com/article/10.1007%2Fs10637-010-9571-7

HeLa cells, SEM - Stock Image G442/0294 - Science Photo LibraryHeLa cells, SEM - Stock Image G442/0294 - Science Photo Library

... of HeLa cells moving through a pore in a filter. HeLa cells were the first continuously cultured human cell line. They were ... However, that are also poorly differentiated and lack some of the characteristics of normal cells, such as the proteins that ... Like almost all cancerous cells they are immortal and so thrive in the laboratory. ... cultivated in 1952 from a cancer of the cervix of Henrietta Lacks (after whom the cells are named) who later died of cervical ...
more infohttp://www.sciencephoto.com/media/214495/view

HeLa cell legal definition of HeLa cellHeLa cell legal definition of HeLa cell

What is HeLa cell? Meaning of HeLa cell as a legal term. What does HeLa cell mean in law? ... Definition of HeLa cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. ... HeLa cell legal definition of HeLa cell https://legal-dictionary.thefreedictionary.com/HeLa+cell ... cell. (redirected from HeLa cell). Also found in: Dictionary, Thesaurus, Medical, Financial, Encyclopedia, Wikipedia. cell. ( ...
more infohttp://legal-dictionary.thefreedictionary.com/HeLa+cell

Direct and indirect immunolabelling of HeLa cells with quantum dots.Direct and indirect immunolabelling of HeLa cells with quantum dots.

... have been made as attractive molecular probes for labelling cells in biological research. In this study high-quality CdSe QDs ... Hela Cells. Humans. Immunoassay / methods*. Molecular Probe Techniques*. Quantum Dots*. Selenium Compounds. ... 972176 - Ultrastructural and enzymic modulation of hela cells induced by sodium butyrate and the.... 9788816 - Protein kinase c ... a cancer marker expressed on the surface of HeLa cells. The primary antibody (Ab) (rabbit anti-CEA8) and secondary Ab (goat ...
more infohttp://www.biomedsearch.com/nih/Direct-indirect-immunolabelling-HeLa-cells/18452136.html

HeLa Cell Dying, SEM Photographic Print by Thomas Deerinck | Art.comHeLa Cell Dying, SEM Photographic Print by Thomas Deerinck | Art.com

HeLa Cell Dying, SEM Photographic Print by Thomas Deerinck. Find art you love and shop high-quality art prints, photographs, ... phagocytosed engulfed and digested by specialist cells HeLa cells are a continuously cultured cell line of human cancer cells ... HeLa cell dying coloured scanning electron micrograph SEM This cell appears spherical because it is undergoing apoptosis or ... programmed cell death Apoptosis occurs when a cell becomes old or damaged Blebs vesicles called apoptotic bodies form on its ...
more infohttps://www.art.com/products/p22105959979-sa-i7520741/thomas-deerinck-hela-cell-dying-sem.htm

Recent Articles | Hela Cells And Neuroscience | The Scientist Magazine®| Page 2Recent Articles | Hela Cells And Neuroscience | The Scientist Magazine®| Page 2

The blood-brain barrier is a collection of specialized cells and proteins that control the movement of molecules from the blood ... The 10-micrometer-long flagellate cell might have a big story to tell about the evolution of eukaryotes. ... Recent advances in single-cell omics and other techniques are revealing variation at genomic, epigenomic, transcriptomic, and ... tags: HeLa cells x neuroscience x The Scientist. » HeLa cells and neuroscience ...
more infohttps://www.the-scientist.com/?articles.list/tagNo/3544,11/tags/HeLa-cells,neuroscience/pageNo/2/

Synthesis of 5S and 4S RNA in Metaphase-Arrested HeLa Cells | ScienceSynthesis of 5S and 4S RNA in Metaphase-Arrested HeLa Cells | Science

Synthesis of 5S and 4S RNA in Metaphase-Arrested HeLa Cells Message Subject. (Your Name) has forwarded a page to you from ... The continued synthesis of both 5S and 4S RNA in metaphase-arrested HeLa cells is demonstrated; 5S RNA is apparently ...
more infohttp://science.sciencemag.org/content/172/3986/947

Stable Trasfection into Hek 293and Hela cells - Cell BiologyStable Trasfection into Hek 293and Hela cells - Cell Biology

Top : Forum Archives: : Cell Biology. Stable Trasfection into Hek 293and Hela cells - (Oct/14/2001 ). Hi, I would like to know ... It is advisable to perform a death curve on the cells to be transfected, for 2 major reasons (even if results for your cell ... Check cells under microscope. The concentration of G418 which just kills all the cells is suitable for use in subsequent ... the right amount of g418 (neomycin) and Hygromycin I have to use, to select stable cell line :Hek 293 and Hela.Thx. ...
more infohttp://www.protocol-online.org/biology-forums/posts/2028.html

HeLa Cells: NIH And Lacks Family Agree On DNA Access : Shots - Health News : NPRHeLa Cells: NIH And Lacks Family Agree On DNA Access : Shots - Health News : NPR

... applications for access to the genetic sequence of cells derived from a tumor that killed Henrietta Lacks. The cells are among ... More than 70,000 published scientific papers mention these cells.. These HeLa cells are so important that several laboratories ... HeLa Cells: NIH And Lacks Family Agree On DNA Access : Shots - Health News A special committee that includes two members of the ... HeLa cells have been mentioned in more than 70,000 published scientific papers. Tom Deerinck/NCMIR hide caption ...
more infohttps://www.npr.org/sections/health-shots/2013/08/07/209807857/decades-after-lacks-death-family-gets-a-say-on-her-cells?ft=1&f=1001

HeLa Cells: NIH And Lacks Family Agree On DNA Access : Shots - Health News : NPRHeLa Cells: NIH And Lacks Family Agree On DNA Access : Shots - Health News : NPR

... applications for access to the genetic sequence of cells derived from a tumor that killed Henrietta Lacks. The cells are among ... More than 70,000 published scientific papers mention these cells.. These HeLa cells are so important that several laboratories ... HeLa Cells: NIH And Lacks Family Agree On DNA Access : Shots - Health News A special committee that includes two members of the ... HeLa cells have been mentioned in more than 70,000 published scientific papers. Tom Deerinck/NCMIR hide caption ...
more infohttps://www.npr.org/sections/health-shots/2013/08/07/209807857/decades-after-lacks-death-family-gets-a-say-on-her-cells
  • They did not interfere with the expression of human papillomavirus oncogenes integrated in the HeLa cell genome, but they did induce strong G1 arrest and result in the activation of caspase-3 and apoptosis. (springer.com)
  • Last March, German scientists published the HeLa genome - again without consent from Henrietta's family - and controversy erupted . (npr.org)
  • The NIH then set up a committee that included the Lacks family to figure out a way to publish the HeLa genome, while still respecting the family's privacy. (npr.org)
  • A portion of the HeLa genome has also been published without familial consent. (labroots.com)
  • This MOA was also confirmed experimentally: the cyano-substituted heteroaryles disrupted the actin and the tubulin networks in HeLa cells and inhibited cellular migration. (springer.com)
  • Bottom row: HeLa cells were treated with low (left) or high (right) concentrations of staurosporine and assayed for cytotoxicity with the EarlyTox Cell Integrity Kit. (moleculardevices.com)
  • Our model was also in agreement with experiments showing that the number of HSF molecules in a HeLa cell is roughly 100 times greater than the number of stress-activated heat-shock element sites, further confirming the model's ability to reproduce experimental results not used in model calibration. (nih.gov)
  • The National Institutes of Health and the Lacks family have agreed to give scientists access to the genetic sequence of the cells, with some restrictions to safeguard her relatives' privacy. (npr.org)
  • The scientists set of a revolution in biomedical research with Lacks' cells, which took their name, HeLa, from hers. (labroots.com)
  • HeLa cells were seeded at 4 initial densities in a 96-well plate: 250 (purple), 500 (blue), 1000 (green), and 2000 (red) cells per well. (moleculardevices.com)
  • HeLa cells were seeded at 1000 cells per well and allowed to grow overnight. (moleculardevices.com)
  • To determine a suitable concentration for selection:Plate non-transfected control cells at a low density (1E4/ml for example)The next day, add G418 at 0, 100, 200, 400, 600, 800, 1000, 1200 ug/mlIncubate the cells for 6 days (may need to refeed)Check cells under microscope. (protocol-online.org)
  • Furthermore, a second in silico investigation revealed that the tumor cells that are sensitive to the cyano-substituted compounds show differences in their expression of locomotion genes compared with that of insensitive cell lines, thus corroborating the involvement of the cytoskeleton. (springer.com)
  • A computational analysis was performed that compared the antiproliferative activities of our compounds in 13 different tumor cell lines with those of compounds listed in the National Cancer Institute database. (springer.com)
  • Working with members of the Lacks family, the University of Washington is the first to publish information on the HeLa cells under the new guidelines. (thefreedictionary.com)
  • HeLa cells have been used extensively around the world in many different fields of research including cancer research, immunology and vaccine development. (wellcomecollection.org)
  • The cell cycle has been extensively studied for years which led to greater understanding of life of a cell and organism. (nature.com)
  • EGFR is a cell surface protein that binds to epidermal growth factor. (novusbio.com)
  • After more than 50 years, there are now billions and billions of HeLa cells in laboratories all over the world. (howstuffworks.com)
  • These HeLa cells are so important that several laboratories have spent years trying to sequence their DNA. (npr.org)
  • Recent advances in single-cell omics and other techniques are revealing variation at genomic, epigenomic, transcriptomic, and posttranscriptomic levels. (the-scientist.com)
  • And while the cells became commercialized (researchers can buy a vial of them for $250) Lacks' family has lived without healthcare and in poverty. (howstuffworks.com)
  • For a long time, researchers who were curious enough to ask were told that HeLa cells were named after 'Helen Lane' or 'Helen Larson. (howstuffworks.com)
  • None of the researchers working on HeLa cells ever took the time to educate the family about what was really going (RadioLab, 2010). (thefreedictionary.com)
  • A biopsy of her cells that was taken during her clinical consultations was later used, without her knowledge, by researchers. (labroots.com)
  • Therefore, we used H2 ER [alpha]-GFP transfected into HeLa cells to test D3 binding by visualizing that D3 increases the translocation of ER [alpha] to the nucleus. (thefreedictionary.com)
  • Decreasing impedance magnitude of the single HeLa cell increases measurement sensitivity, but higher operational voltage will damage single HeLa cell. (hindawi.com)
  • The latter interaction is essential for kinetochore localization of Bub1 and its function in cell cycle arrest induced by spindle assembly checkpoint (SAC) activation. (wikipedia.org)
  • All of the body's normal cells experience the effects of aging over time, known as cellular senescence . (howstuffworks.com)
  • The cell cycle is a universal process during which, through a series of cellular events, single cell gives rise to two daughter individuals. (nature.com)
  • At the individual cell level, the electrical properties of the cell are helpful for understanding the effects of cellular behavior. (hindawi.com)
  • C )MS/MS/MS spectrum of the neutral loss precursor ion from B . Abundant peptide bond fragmentation permitted the unambiguous identification of this peptide from the protein cell division cycle 2-related protein kinase 7, with a phosphorylated Ser residue marked by an asterisk. (pnas.org)
  • The present study demonstrates the practicability of CdSe QDs as an attractive type of fluorescent labels for biological applications such as protein probes and cell imaging. (biomedsearch.com)
  • The protein kinase Bub1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase. (wikipedia.org)
  • The concentration of G418 which just kills all the cells is suitable for use in subsequent selection procedures. (protocol-online.org)
  • Once the killer cell has killed some of the HeLa cells, the killer cell is gently detached from the HeLa layer with the CellEctor microcapillary for subsequent molecular analysis. (selectscience.net)
  • Nanoviscosity of the cytoplasm is a key factor affecting diffusion of biomolecules and - as a consequence - rates of biochemical reactions in a cell. (nature.com)
  • In this paper we address this topic by constant observation of nanoviscosity of HeLa cells cytoplasm during S, G2 and G1 phases after Aphidicolin synchronization. (nature.com)
  • Our results hint at possible existence of robust mechanism maintaining stable physiological viscosity of the cytoplasm, despite huge structural changes during cell cycle. (nature.com)
  • Cell lines are used in all kinds of ways, such as studying the effects of diseases or developing medications and vaccines, and play an invaluable role in medicine today. (howstuffworks.com)
  • 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines. (novusbio.com)
  • The cell cycle of somatic cells is commonly divided into two periods: interphase and mitosis. (nature.com)
  • It was expected, that significant structural changes in cells during biosynthesis or mitosis would have an impact on nanoviscosity, and further - diffusive motion of biomolecules. (nature.com)
  • In humans Bub1 accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. (wikipedia.org)
  • In the cell in the centre of the image, condensed chromosomes (purple) have aligned and attached to the spindle (green). (wellcomecollection.org)
  • HeLa cells are incredibly hardy, which makes them very useful for medical research. (howstuffworks.com)
  • The fact that HeLa cells have been used in some very important, groundbreaking medical research is interesting enough, but there's another part of the story -- and that part is why Oprah might be making a movie about HeLa. (howstuffworks.com)
  • With excellent optical properties, quantum dots (QDs) have been made as attractive molecular probes for labelling cells in biological research. (biomedsearch.com)
  • The cells are among the most widely used in research. (npr.org)
  • These cells are among the most widely used in biomedical research worldwide. (npr.org)
  • Those unique and important cells were the first to be cultured and reproduced in a laboratory. (labroots.com)
  • For example I found out that the test my daughter had to confirm a diagnosis of Williams Syndrome was made possible by HeLa cells ," she says. (thefreedictionary.com)
  • Dr. Gey had been trying to grow human cells in the lab for decades, but they always died within a few days. (howstuffworks.com)