A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.
Established cell cultures that have the potential to propagate indefinitely.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
An eph family receptor that is found primarily in adult BRAIN and variety of tissues in the developing embryo tissues. During embryonic development high levels of EphA3 receptor expression is seen in the nervous system and coincides with neuronal cell migration, suggesting a role for this protein in axonal pathfinding.
An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins prepared by recombinant DNA technology.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The opening and closing of ion channels due to a stimulus. The stimulus can be a change in membrane potential (voltage-gated), drugs or chemical transmitters (ligand-gated), or a mechanical deformation. Gating is thought to involve conformational changes of the ion channel which alters selective permeability.
A subgroup of TRP cation channels that contain 3-4 ANKYRIN REPEAT DOMAINS and a conserved C-terminal domain. Members are highly expressed in the CENTRAL NERVOUS SYSTEM. Selectivity for calcium over sodium ranges from 0.5 to 10.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution.
Regulatory proteins that down-regulate phosphorylated G-protein membrane receptors, including rod and cone photoreceptors and adrenergic receptors.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
The rate dynamics in chemical or physical systems.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).
A family of voltage-gated potassium channels that are characterized by long N-terminal and C-terminal intracellular tails. They are named from the Drosophila protein whose mutation causes abnormal leg shaking under ether anesthesia. Their activation kinetics are dependent on extracellular MAGNESIUM and PROTON concentration.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
A subgroup of TRP cation channels named after vanilloid receptor. They are very sensitive to TEMPERATURE and hot spicy food and CAPSAICIN. They have the TRP domain and ANKYRIN repeats. Selectivity for CALCIUM over SODIUM ranges from 3 to 100 fold.
A purinergic P2X neurotransmitter receptor that plays a role in pain sensation signaling and regulation of inflammatory processes.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Potassium channels where the flow of K+ ions into the cell is greater than the outward flow.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.
A family of proteins involved in the transport of organic cations. They play an important role in the elimination of a variety of endogenous substances, xenobiotics, and their metabolites from the body.
A class of G-protein-coupled receptors that react to varying extracellular CALCIUM levels. Calcium-sensing receptors in the PARATHYROID GLANDS play an important role in the maintenance of calcium HOMEOSTASIS by regulating the release of PARATHYROID HORMONE. They differ from INTRACELLULAR CALCIUM-SENSING PROTEINS which sense intracellular calcium levels.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Cell membrane glycoproteins that are selectively permeable to potassium ions. At least eight major groups of K channels exist and they are made up of dozens of different subunits.
Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Ion channels that specifically allow the passage of SODIUM ions. A variety of specific sodium channel subtypes are involved in serving specialized functions such as neuronal signaling, CARDIAC MUSCLE contraction, and KIDNEY function.
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.
An eph family receptor found in a number of tissues including BRAIN; LUNG; KIDNEY; PANCREAS; INTESTINE; and HEART. During embryogenesis EphB3 receptor is expressed at high levels in the brain.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
A subclass of serotonin receptors that form cation channels and mediate signal transduction by depolarizing the cell membrane. The cation channels are formed from 5 receptor subunits. When stimulated the receptors allow the selective passage of SODIUM; POTASSIUM; and CALCIUM.
A subgroup of TRP cation channels named after melastatin protein. They have the TRP domain but lack ANKYRIN repeats. Enzyme domains in the C-terminus leads to them being called chanzymes.
A voltage-gated sodium channel subtype that mediates the sodium ion PERMEABILITY of CARDIOMYOCYTES. Defects in the SCN5A gene, which codes for the alpha subunit of this sodium channel, are associated with a variety of CARDIAC DISEASES that result from loss of sodium channel function.
Incorporation of biotinyl groups into molecules.
Potassium channel whose permeability to ions is extremely sensitive to the transmembrane potential difference. The opening of these channels is induced by the membrane depolarization of the ACTION POTENTIAL.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A family of heterotrimeric GTP-binding protein alpha subunits that activate TYPE C PHOSPHOLIPASES dependent signaling pathways. The Gq-G11 part of the name is also spelled Gq/G11.
A secreted protein that associates with TOLL-LIKE RECEPTOR 4 and is essential for receptor recognition of LIPOPOLYSACCHARIDES.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed)
A delayed rectifier subtype of shaker potassium channels that conducts a delayed rectifier current. It contributes to ACTION POTENTIAL repolarization of MYOCYTES in HEART ATRIA.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
A pattern recognition receptor that forms heterodimers with other TOLL-LIKE RECEPTORS. It interacts with multiple ligands including PEPTIDOGLYCAN, bacterial LIPOPROTEINS, lipoarabinomannan, and a variety of PORINS.
Transport proteins that carry specific substances in the blood or across cell membranes.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.

Mammalian Notum induces the release of glypicans and other GPI-anchored proteins from the cell surface. (1/10093)

Glypicans are heparan sulfate proteoglycans that are attached to the cell surface by a GPI (glycosylphosphatidylinositol)anchor. Glypicans regulate the activity of Wnts, Hedgehogs,bone morphogenetic proteins and fibroblast growth factors. In the particular case of Wnts, it has been proposed that GPI-anchored glypicans stimulate Wnt signalling by facilitating and/or stabilizing the interaction between Wnts and their cell surface receptors. On the other hand, when glypicans are secreted to the extracellular environment, they can act as competitive inhibitors of Wnt. Genetic screens in Drosophila have recently identified a novel inhibitor of Wnt signalling named Notum. The Wnt inhibiting activity of Notum was associated with its ability to release Dlp [Dally (Division abnormally delayed)-like protein; a Drosophila glypican] from the cell surface by cleaving the GPI anchor. Because these studies showed that the other Drosophila glypican Dally was not released from the cell surface by Notum,it remains unclear whether this enzyme is able to cleave glypicans from mammalian cells. Furthermore, it is also not known whether Notum cleaves GPI-anchored proteins that are not members of the glypican family. Here, we show that mammalian Notum can cleave several mammalian glypicans. Moreover, we demonstrate that Notum is able to release GPI-anchored proteins other than glypicans. Another important finding of the present study is that,unlike GPI-phospholipase D, the other mammalian enzyme that cleaves GPI-anchored proteins, Notum is active in the extracellular environment. Finally, by using a cellular system in which GPC3 (glypican-3) stimulates Wnt signalling, we show that Notum can act as a negative regulator of this growth factor.  (+info)

alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clustering. (2/10093)

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Influence of brain-derived neurotrophic factor on pathfinding of dentate granule cell axons, the hippocampal mossy fibers. (3/10093)

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Effective gene-viral therapy of leukemia by a new fiber chimeric oncolytic adenovirus expressing TRAIL: in vitro and in vivo evaluation. (4/10093)

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A novel human monoclonal antibody that binds with high affinity to mesothelin-expressing cells and kills them by antibody-dependent cell-mediated cytotoxicity. (5/10093)

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Regulation of dopamine transporter activity by carboxypeptidase E. (6/10093)

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Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models. (7/10093)

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Selenoprotein P regulation by the glucocorticoid receptor. (8/10093)

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HLA A*0201 WT 1 complex Protein Human HEK293 His best suppliers; HLA A*0201 WT 1 complex Protein Human HEK293 His best sources; HLA A*0201 WT 1 complex Protein Human HEK293 His best vendors; HLA A*0201 WT 1 complex Protein Human HEK293 His protocol; HLA A*0201 WT 1 complex Protein Human HEK293 His citations; HLA A*0201 WT 1 complex Protein Human HEK293 His publications; HLA A*0201 WT 1 complex Protein Human HEK293 His papers - Labshake
COPS2 antibody - N-terminal region (ARP38431_T100) | Application: IHC, WB | COPS2 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells | Alias: ALIEN; CSN2; SGN2; TRIP15
Flt3-Ligand Human Recombinant produced in HEK293 cells is a glycosylated, polypeptide chain, migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30kDa.
Recombinant human ENPP1 Protein is a 840 amino acid protein expressed in HEK293. Tested applications are: Cell culture and/or animal studies, Western blotting, ELISA.
How to treat HEK293 with S9 fraction? - posted in Tissue and Cell Culture: Hi, everyone In my experiment, i need to treat HEK293 cells with drug including S9 fraction. In the protocol of S9. The medium including S9 have to remove and wash with PBS after treating in 4 hours. But the adhesion ability of HEK293 cells are very week. The cells will be lost if i wash them. What can i do to improve it? Thanks a lot.
Human Nectin-2 HEK293 Cells Overexpression Lysate 10005-HCCHL is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
Human IL-8/CXCL8 HEK293 Cells Overexpression Lysate 10098-HNCH1L is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
Mouse IL3RA HEK293 Cells Overexpression Lysate 50810-M02HL is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
I was wondering if my transfections are not working because Im using the wrong cell line, I have been using HEK293, but is HEK293T better for transient and stable transfection? Also is there a difference with vendors (ie, some have better cell lines? lower passages perhaps?). Im just getting frustrated with these transfections that do not work. If anyone has any suggestions I would greatly appreciate it ...
Localization of FAM20A to the Golgi.HEK293 cells were co-transfected with two plasmids. Cells that expressed the Golgi-GFP exhibited green fluorescence in the G
BioAssay record AID 478847 submitted by ChEMBL: Antagonist activity at rat Cav1.3 expressed in HEK293 cells assessed as inhibition of voltage pulse-induced calcium current at 1000 nM by FLIPR calcium 4 assay.
Human HRG/HPRG HEK293 Overexpression Lysate | SinoBiological, 10836-H08HL is used as western blot (WB) positive control. Bulk order, Produced in house, High quality guarrantee.
Use Human IL1R1(Fc) HEK293 Cell Lysate for fast, easy, and consistent DNA/RNA Purification, Antibody/Protein Purification, Cell Isolation.
Use Human TIMP1 HEK293 Cell Lysate for fast, easy, and consistent DNA/RNA Purification, Antibody/Protein Purification, Cell Isolation.
Alimak Hek is the worlds leading provider of rack and pinion vertical access equipment for both temporary and permanent installations. We provide a number of solutions and continued services through after sales support.
购买我们的重组人ANGPTL3蛋白。Ab109135为有活性的全长蛋白,在HEK 293细胞中生产并经过Functional Studies, SDS-PAGE实验验证。中国80%以上现货。
Human embryonic kidney cells 293, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy. HEK 293 cells were generated in 1973 by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Ebs laboratory in Leiden, the Netherlands. The cells were obtained from a single, apparently healthy, legally aborted fetus under Dutch law; the identity of the parent and the reason for the abortion are unknown. The cells were cultured by van der Eb; the transformation by adenovirus was performed by Frank Graham, a post-doc in van der Ebs lab. They were published in 1977 ...
BioAssay record AID 646449 submitted by ChEMBL: Displacement of [3H]-LSD from human full length cloned 5HT6 receptor expressed in human HEK293 cells by liquid scintillation counter.
Activation of the inflammasome, a complex that processes interleukin-1β (IL-1β), occurs in response to activation of the adenosine triphosphate receptor P2X7 on macrophages. Activation of P2X7 also triggers the opening of a nonselective, large pore in the plasma membrane. Pelegrin and Surprenant found that pannexin-1 (panx1), a protein that produces hemichannel currents when ectopically expressed in oocytes, was present in monocytes, macrophages, astrocytes, and various cell lines. Panx1, when expressed in transfected HEK cells, coimmunoprecipitated with the P2X7 receptor and colocalized with the P2X7 receptors in ATP-stimulated cells. Using either an siRNA to knock down panx1 or a peptide inhibitor to block the pore function of panx1, the authors showed that dye uptake (a measure of pore opening) in response to P2X7 required panx1. When overexpressed in cells that lack P2X7 receptors, panx1 caused constitutive dye uptake, and when overexpressed in cells that contain P2X7 receptors, panx1 ...
Dear all, I have some stably transfected HEK cells, which secrete my favorite protein which is the extracellular part of a receptor. I want to produce pseudo-industrial amounts of my protein and I was wondering how to optimise production/secretion. Is it best to grow the cells to a certain density and then apply serum-free medium for a given amount of time and harvest it, or is a continuous exchange of conditioned medium from confluent cells to prefer ? Any inputs appreciated, Thanks /svend -- *************************************** Svend Kjær, Ph.D. Division of Molecular Neurobiology, Department of Neuroscience Karolinska Institute Berzelius väg 1, 3rd floor 171 77 Stockholm Sweden Tel: +46-8-7287666 Fax: +46-8-339548 Private phone: +46-8-6409962 ...
Every newb thinks that a technique they havent mastered is easy, until they actually try it. And in fact, the bare bones mechanics of patching are pretty straightforward. Ive taught a lot of novices how to patch, and by and large they can get to the point of gigaohm seals in a week or two (ok, were talking transfected HEK cells here). Hell, Im thinking any primate above lemurs could learn to get seals. (Not a bad idea actually; screw those automated patch systems, gimme an army of squirrel monkeys and an old warehouse, and Ill screen your chemical library right quick! Itd be like the nut shelling squirrels in Willy Wonka. And theyd literally be DrugMonkeys! LOLZ ...
Every newb thinks that a technique they havent mastered is easy, until they actually try it. And in fact, the bare bones mechanics of patching are pretty straightforward. Ive taught a lot of novices how to patch, and by and large they can get to the point of gigaohm seals in a week or two (ok, were talking transfected HEK cells here). Hell, Im thinking any primate above lemurs could learn to get seals. (Not a bad idea actually; screw those automated patch systems, gimme an army of squirrel monkeys and an old warehouse, and Ill screen your chemical library right quick! Itd be like the nut shelling squirrels in Willy Wonka. And theyd literally be DrugMonkeys! LOLZ ...
Thrombopoietin Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 80-85kDa due to glycosylation.
Recombinant human Adiponectin is a 237 amino acid protein expressed in HEK293 Solution. Tested applications are: Cell culture and/or animal studies, Western blotting, ELISA.
What ist the difference between Hek293 and Hek293T cells? Thank you very much Antonio -- Antonio Sarikas Institut für Klin. und Exp. Pharmakologie und Toxikologie Universität Erlangen-Nürnberg Fahrstrasse 17, D-91054 Erlangen Tel: +49 (0)9131 - 85-22783 Fax: +49 (0)9131 - 85-22774 Email: Sarikas at pharmakologie.uni-erlangen.de ...
购买我们的重组人PLGF蛋白。Ab84148为全长蛋白,在HEK 293细胞中生产并经过SDS-PAGE实验验证。Abcam提供免费的实验方案,操作技巧及专业的支持。中国80%以上现货。
HEK 293 cells were generated by transformation of human embryonic kidney cell cultures (hence HEK) with sheared adenovirus 5 DNA, and were first described in 1977 (Graham et al., J. Gen Virol 1977 Jul;36(1):59-74). The story of this cell line is quite an interesting one, getting to the very roots of modern molecular biology. Frank Graham was working with Alex Van der Eb in the University of Leiden in Holland, and developed the calcium phosphate method of transfecting eukaryotic cells, an astonishing technique described in a classic paper (Graham and Van der Eb. Virology 1973 Apr;52(2):456-67); who would have thought that you would only have to mix DNA with a bit of calcium phosphate to get the DNA incorporated and even expressed in mammalian cells? The story of this discovery was described by Graham himself for Current Contents in 1988 (link to *.pdf here). By 1977 Graham had moved to Canada, and from there produced the other classical paper describing the derivation of the HEK 293 cells. There ...
HEK 293 cells were generated by transformation of human embryonic kidney cell cultures (hence HEK) with sheared adenovirus 5 DNA, and were first described in 1977 (Graham et al., J. Gen Virol 1977 Jul;36(1):59-74). The story of this cell line is quite an interesting one, getting to the very roots of modern molecular biology. Frank Graham was working with Alex Van der Eb in the University of Leiden in Holland, and developed the calcium phosphate method of transfecting eukaryotic cells, an astonishing technique described in a classic paper (Graham and Van der Eb. Virology 1973 Apr;52(2):456-67); who would have thought that you would only have to mix DNA with a bit of calcium phosphate to get the DNA incorporated and even expressed in mammalian cells? The story of this discovery was described by Graham himself for Current Contents in 1988 (link to *.pdf here). By 1977 Graham had moved to Canada, and from there produced the other classical paper describing the derivation of the HEK 293 cells. There ...
Purified Recombinant Human KAT2A HEK293T cell lysate from Creative Biomart. Recombinant Human KAT2A HEK293T cell lysate can be used for research.
H1N1 Hemagglutinin/HA HEK293 Cells Overexpression Lysate 40394-V08H1L is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
H5N1 Hemagglutinin/HA HEK293 Cells Overexpression Lysate 11710-V08H1L is validated in western blot (WB) as positive control. Sino Biological offers bulk order for high quality cell lysates which are produced in house.
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EPCRAVFREAEVTLEAGGAEQEPGQALGKVFMGCPGQEPALFSTDNDDFTVRNGETVQERRSLKERNPLKIFPSKRILRRHKRDWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQVTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRLTVTDLDAPNSPAWRATYLIMGGDDGDHFTITTHPESNQGILTTRKGLDFEAKNQHTLYVEVTNEAPFVLKLPTSTATIVVHVEDVNEAPVFVPPSKVVEVQEGIPTGEPVCVYTAEDPDKENQKISYRILRDPAGWLAMDPDSGQVTAVGTLDREDEQFVRNNIYEVMVLAMDNGSPPTTGTGTLLLTLIDVNDHGPVPEPRQITICNQSPVRQVLNITDKDLSPHTSPFQAQLTDDSDIYWTAEVNEEGDTVVLSLKKFLKQDTYDVHLSLSDHGNKEQLTVIRATVCDCHGHVETCPGPWKGGHHHHHH ...
Looking for online definition of HEK2 or what HEK2 stands for? HEK2 is listed in the Worlds largest and most authoritative dictionary database of abbreviations and acronyms
Purified PTGER4 HEK 293 β-Arrestin GPCR Assay Kit from Creative Biomart. PTGER4 HEK 293 β-Arrestin GPCR Assay Kit can be used for research.
Transient tranfections of pMech_EGFP constructs were performed on HEK293FT cells seeded at 2 million cells/well in two identically seeded six-well plates. The constructs used were: pCOFS_EGFP, pPAI-2_EGFP, pSRE/CRE2_SV40_EGFP, pNR1/2_SV40_EGFP, and CMV_EGFP. Of these CMV_EGFP served as a control for transformation efficiency. 12 hours after transfection one of the two six well plates was designated as shear stress and placed on a shaker at 120 rpm inside the incubator. The other plate was designated control and not subjected to shaking. Fluorescent micrographs were obtained on a Zeiss microscope for each well at 100, 215, and 500 ms exposure times every 3 hours. Brightfield images were exposed for 6 ms. Images were exported from the accompanying Axiovision software at identical histogram levels for each cell line. For image analysis, accurate cell count could not be obtained because HEK cells grow at high density. Instead area occupied by cells was used as an analogous measurement for fields ...
The cGAS-STING cytosolic DNA sensing pathway mediates recognition of MHV-68 in MSCs.MSCs were infected with MHV-68 (MOI 0.1) (a) or transfected with MHV-68 DNA
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Recombinant Human Her2/ERBB2 Protein (Pro489-Cys630) ECD, domain IV 10004-H08H4 with a fusion His Tag, is expressed in HEK293 Cells. With high purity, high biological activity, high stability, and other superior features, you can use this Human Her2/ERBB2 protein for relevant bioassay and related research.
01:07, 18 November 2012 (diff , hist) . . (+1,003)‎ . . N Structural Biochemistry/DNA recombinant techniques/293T Cells ‎ (Created page with =293T Cells= 293T cells, or Human Embryonic Kidney (HEK) 293 cells, are cells that are derived from human embryonic kidney cells. These cells are very tough and can be cultu...) ...
MedChemExpress offers high purity Siglec-15 Protein, Cynomolgus (HEK 293, His) with excellent lot-to-lot consistency, superior biological activity and low endotoxin levels.
Transient overexpression of CBR3 (NM_001236) in HEK293T cells paraffin embedded 4 um sections, controls for ICC/IHC staining, 5 slides per pack
Supplementary MaterialsFigure 1source data 1: Resource data indicating that BimL requires a CTS to induce MOMP in HEK293 cells. to membranes. elife-44525-fig4-data1.xlsx (18K) GUID:?6217D04D-BD0F-4FF4-97F3-EA7D1AC332EC Figure 5source data 1: Source data demonstrating that recombinant BimL-dCTS indirectly activates Bax through inhibition of Bcl_XL. elife-44525-fig5-data1.xlsx (15K) GUID:?2FBDE8FA-CD3F-4B89-86F2-CB6EDE9F5481 Figure 6figure supplement 1source data 1: Source data with fitted curves used to calculate dissociation constants Ntn1 and EC50s for Figure 6B. elife-44525-fig6-figsupp1-data1.xlsx (52K) GUID:?39E7B383-B55E-415D-BD93-37BD0557E842 Figure 7source data 1: Source data demonstrating that BimL-dCTS-MAO binds to liposomes and Bax, but activates Bax poorly. elife-44525-fig7-data1.xlsx (18K) GUID:?47A95C70-2208-4738-8FA6-F19BF88AA00F Figure 7figure supplement 1source data 1: Source data demonstrating BimL-dCTS-MAO binds to mitochondria but binds poorly to Bax. ...
Western blot analysis in control (vector only transfected HEK293T lysate) and ALKBH3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403381 ...
Optimized DNA sequence encoding mature chain of Human B7-H4 (PHE29 - ALA258) including a C-terminal His tag was expressed in HEK293 cells.
The G-protein-coupled relaxin family receptors RXFP1 and RXFP3 are widely expressed in the cortex and are involved in stress responses and memory and emotional processing. However, the identification of these receptors in human cortex and their status in Alzheimers disease (AD), which is characterized by both cognitive impairments and neuropsychiatric behaviours, have not been reported. In this study, we characterized RXFP receptors for immunoblotting and measured RXFP1 and RXFP3 immunoreactivities in the postmortem neocortex of AD patients longitudinally assessed for depressive symptoms. RXFP1 and RXFP3 antibodies were characterized by immunoblotting with lysates from transfected HEK cells and preadsorption with RXFP3 peptides. Also, postmortem neocortical tissues from behaviourally assessed AD and age-matched controls were processed for immunoblotting with RXFP1 and RXFP3 antibodies. Compared to controls, putative RXFP1 immunoreactivity was reduced in parietal cortex of non-depressed AD ...
Hari pertama ibu dgn Aniq. Mmg totally tatau pape. Sblum bersalin ada membaca psal susu ibu, tp bila nak praktis, blur terus, tatau ape nak buat dulu. Misi pulak hantar Aniq pukul 5 pg, 12 hours after he was born. Mujur Aniq budak baik, tak menangis walau lapar susu. Bunyi hek hek hek je. Akak sebelah katil yg gtau suh susukan sbb baby mcm lapar. Cuba 2-3 kali, Aniq mcm tak boleh nak menyusu. Susu mcm takde. Latching pom xbrape betul. Give up. Byk kali give up. Tp Aniq bdk baik, dia tak rungsingkan ibu. Tak nangis, masih hek hek hek. Ibu try lg. Misi taim tu mmg tak membantu. Suh bg susu, tp tak bantu tunjukkan cara. Hari kedua, masih belum terasa ada susu. Tp bg jugak Aniq isap. Hari ketiga, baru nampak pipi die kembong minum susu. B pom terasa berat dgn susu. Alhamdulillah. Mmg 1st 2nd day, susu blum ada lg, tp try susukan jugak sbb itu yg merangsang penghasilan susu. ...
Anti-CRBN Antibody, clone 3I23 ZooMAb® Rabbit Monoclonal recombinant, expressed in HEK 293 cells; find Sigma-Aldrich-ZRB1304 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
DİCLE DİŞHEKİMLİĞİ DERGİSİ DENTAL JOURNAL OF DİCLE Cilt / Volume 12 Sayı / Number Sahibi Prof. Dr. Remzi NİGİZ Dicle Üniversitesi Dişhekimliği Fakültesi
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With Graham's contributions in transfection of the cells with the adenovirus 5 this led to the cell line of HEK 293 cells. HEK ... "HEK293 Cell Line". hek293.com. Archived from the original on 16 October 2014. "In memoriam professor Mathieu Noteborn" (in ... he created the PER.C6 cell line with enhanced traceability compared to the HEK 293 line, this time coming from retinal cells. ... In contrast to HEK 293 the PER.C6 cell line was patented by Leiden University and IntroGene (later Crucell) and used ...
This was verified using GPR18 transfected HEK-293 cells. The same migration wasn't witnessed using non-transfected and GPR55 ... NAGly has been hypothesized to induce cell migration in BV-2 microglia cells. The same research suggests that this migration ... NAGly powerfully stimulates oxygen consumption in multiple cell lines, including murine C2C12 myoblasts and human HEK293T cells ... Cell. 166 (2): 424-435. doi:10.1016/j.cell.2016.05.071. PMC 4947008. PMID 27374330. Lin H, Long JZ, Roche AM, Svensson KJ, Dou ...
2007). "Upregulation of PHLDA2 in Dicer knockdown HEK293 cells". Biochim. Biophys. Acta. 1770 (5): 820-5. doi:10.1016/j.bbagen. ...
"Subcellular localization of human neutral ceramidase expressed in HEK293 cells". Biochemical and Biophysical Research ...
2005). "Subcellular localization of human neutral ceramidase expressed in HEK293 cells". Biochem. Biophys. Res. Commun. 331 (1 ... 2006). "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P". ...
"Functional comparison of HCN isoforms expressed in ventricular and HEK 293 cells". Pflügers Archiv. 444 (5): 597-601. doi: ...
The virus is grown in HEK 293 cells and purified for administration. Married researchers Jean Bennett and Albert Maguire, among ... due to inherited retinal dystrophy caused by confirmed biallelic RPE65 mutations and who have sufficient viable retinal cells. ...
Knock down of SIRT7 in HEK293 cells resulted in decreased rRNA levels. This same study found that this SIRT3 knockdown ... In humans cells, SIRT7 has only been shown to interact with two other molecules: RNA polymerase I (RNA Pol I) and upstream ... Mohrin M, Shin J, Liu Y, Brown K, Luo H, Xi Y, Haynes CM, Chen D (Mar 2015). "Stem cell aging. A mitochondrial UPR-mediated ... These features include premature curvature of the spine, reduced weight and fat content, compromised hematopoietic stem cell ...
"Functional comparison of HCN isoforms expressed in ventricular and HEK 293 cells". Pflügers Archiv. 444 (5): 597-601. doi: ... Calloe K, Elmedyb P, Olesen SP, Jorgensen NK, Grunnet M (Sep 2005). "Hypoosmotic cell swelling as a novel mechanism for ... Cell. 93 (5): 717-29. doi:10.1016/S0092-8674(00)81434-8. PMID 9630217. S2CID 10265917. Hofmann F, Biel M, Kaupp UB (Dec 2005 ... "Identification of the cyclic-nucleotide-binding domain as a conserved determinant of ion-channel cell-surface localization". ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... "Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells". Mol. Med. 8 (2): 95- ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. Guo D ... "A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death". ... 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ...
2006). "Acute hypoxic regulation of recombinant THIK-1 stably expressed in HEK293 cells". Adv. Exp. Med. Biol. ADVANCES IN ... 2008). "Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1) K channels in the mammalian kidney". Cell. Physiol. ...
December 2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and ...
December 2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and ... in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells". Molecular and Cellular Biology ... "Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines". Oncogene. 17 (10): ... Cell. 96 (5): 645-53. doi:10.1016/S0092-8674(00)80575-9. PMID 10089880. S2CID 17117789. Deng L, Wang C, Spencer E, Yang L, ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. Rual ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... Cell. Biol. 20 (9): 3157-67. doi:10.1128/MCB.20.9.3157-3167.2000. PMC 85610. PMID 10757800. Zhang QH, Ye M, Wu XY, Ren SX, Zhao ... Cell. Biol. 20 (9): 3157-67. doi:10.1128/mcb.20.9.3157-3167.2000. PMC 85610. PMID 10757800. Datta PK, Chytil A, Gorska AE, ... "High-throughput mapping of a dynamic signaling network in mammalian cells". Science. 307 (5715): 1621-5. doi:10.1126/science. ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... Molecular Cell. 5 (5): 779-87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114. Will CL, Schneider C, MacMillan AM, Katopodis ... "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94-101. doi:10.1038/ ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... "Specific association of estrogen receptor beta with the cell cycle spindle assembly checkpoint protein, MAD2". Proc. Natl. Acad ... "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94-101. doi:10.1038/nbt1046. ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... "Identification of candidate radioresistant genes in human squamous cell carcinoma cells through gene expression analysis using ... Cell Biol. 6 (2): 97-105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986. Higo M, Uzawa K, Kouzu Y, Bukawa H, Nimura Y, ... Cell. Proteomics. 4 (11): 1725-40. doi:10.1074/mcp.M500231-MCP200. PMID 16085932. Human CCT6A genome location and CCT6A gene ...
"Synthetic ciguatoxins selectively activate Nav1.8-derived chimeric sodium channels expressed in HEK293 cells". The Journal of ... Voltage clamp has been used to show how action potentials in DRG cells are shaped by TTX-resistant sodium channels. Nav1.8 ... Lipid rafts organise the cell membrane, which includes trafficking and localising ion channels. Removal of lipid rafts in the ... Although NaV1.8 is not normally expressed within the cerebellum, its expression is up-regulated in cerebellar Purkinje cells in ...
December 2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and ... Within cells, IDH1 has been observed to localize to the cytoplasm, peroxisome, and endoplasmic reticulum. Under hypoxic ... In addition, IDH1 is key to β-oxidation of unsaturated fatty acids in the peroxisomes of liver cells. IDH1 also participates in ... Vaccination of MHC-humanized transgenic mice with mutant IDH1 peptide induced an IFN-γ CD4+ T-helper 1 cell response, ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... "The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells". Molecular Biology of the Cell. 9 ... "Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells". The Journal of Cell Biology. 143 (6): ... Gangwani L (December 2006). "Deficiency of the zinc finger protein ZPR1 causes defects in transcription and cell cycle ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... IDH3 activity is regulated by the energy needs of the cell: when the cell requires energy, IDH3 is activated by ADP; and when ... Within cells, IDH3 and its subunits have been observed to localize to the mitochondria. IDH3α expression has been linked to ... HIF-1 is largely known for shifting glucose metabolism from oxidative phosphorylation to aerobic glycolysis in cancer cells ( ...
"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... "A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death". ... 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell ...
2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. ... 2008). "Direct role of nucleotide metabolism in C-MYC-dependent proliferation of melanoma cells". Cell Cycle. 7 (15): 2392-400 ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ... doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685. He Y, Mou Z, Li W, et al. (2009). "Identification of IMPDH2 as a ...
"Chelerythrine chloride induces apoptosis in renal cancer HEK-293 and SW-839 cell lines". Oncology Letters. 11 (6): 3917-3924. ... This molecule has the ability to disrupt a bacteria's cell wall and cell membrane, as well as preventing bacterial growth, all ... This further contributes to the loss of calcium ion balance within the cell and eventual cell death. Previous studies have ... Analogues designed by molecular simplification exhibit selective growth inhibition in non-small-cell lung cancer cells". ...
When expressed in naïve HEK293 cells, synphilin 1 forms multiple small highly mobile aggregates. However, proteasome or Hsp90 ... In eukaryotic cells, an aggresome refers to an aggregation of misfolded proteins in the cell, formed when the protein ... Consequently, elaborate systems have evolved to protect cells from the deleterious effects of misfolded proteins. Cells mainly ... An aggresome forms around the microtubule organizing center in eukaryotic cells, adjacent to or enveloping the cell's ...
2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. ... Cell. 16 (11): 5445-5454. doi:10.1091/mbc.E05-04-0354. PMC 1266439. PMID 16148047. Guo D, Han J, Adam BL, et al. ( ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Frum R, Busby SA, Ramamoorthy M, et al. (2007). "HDM2-binding ... 2005). "The glutamine-rich region of the HIV-1 Tat protein is involved in T-cell apoptosis". J. Biol. Chem. 279 (46): 48197- ...
2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. ...
As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. HEK 293 cells have a complex ... HEK 293 Transfection and Selection Data @ Cell-culture Database A HEK293 Cell Database 293 Cells (CRL-1573) in the ATCC ... Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a ... HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity ...
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HEK293 whole cell lysate Lysates datasheet (ab7902). Abcam offers quality products including antibodies, assays and other ... The positive control is HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902). Please refer to the Cell Lysate ... Cell line: HEK293 (Human embryonic kidney cell line).. Growth media: RPMI and 10% FBS (Fetal bovine serum).. ... "HEK293 Human embryonic kidney cell line Whole Cell Lysate" instead of liver or pancreas tissue lysate as a positive control for ...
Top : Forum Archives: : Cell Biology. HEK293 vs HEK293T - which is better for transfection (May/13/2008 ). I was wondering if ... my transfections are not working because Im using the wrong cell line, I have been using HEK293, but is HEK293T better for ... I don`t think there would be a big difference between those two cell lines regarding transfection. It would be better if they ... Are you sure that your cells are nice plated and looking happy when you transfect?. You could give some more details so that we ...
... cells are a family of reporter cells designed to study variants of STING (stimulator of interferon genes; also known as TMEM173 ... HEK STING Reporter Cells. STING SEAP Reporter 293. 293-Dual™ STING (ISG/KI-IFNb) cells are a family of reporter cells designed ... 293-Dual™ STING (ISG/KI-IFNb) cells were generated from 293-Dual™ Null (ISG/KI-IFNb) cells, which derive from 293 cells, human ... hSTING-R232 Cells Dual IRF and IFN-β reporter 293 cells expressing R232 isoform of human STING 293-Dual™ mSTING Cells Dual IRF ...
... or HEK cells, are a cell line from human embryonic kidney. HEK293 cells are used extensively in transfection-based experiments ... Human Embryonic Kidney 293 cells, also known as HEK293, 293 cells, or even as HEK cells, are a cell line derived from human ... HEK293-GFP cells seeded at 20000, 5000, and 1250 cells per well and imaged in the green fluorescence channel of the MiniMax ... HEK293-GFP cells were imaged using the SpectraMax i3 MiniMax 300 Imaging Cytometer, and cells were identified using a custom ...
... expressed in HEK 293 cells, ≥97% (SDS-PAGE); Synonym: FNDC5 (cleaved), Fibronectin Type III Domain-containing Protein 5 ( ... recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE) Synonym: FNDC5 (cleaved), Fibronectin Type III Domain-containing ...
transfection of HEK293 cells I have been trying to transfect HEK293 cells with Transfast and Lipofectamine and have not been ... i have a question regarding confluency of HEK293 cells for transfection.Yous uggested to thaw a new vial of cells if the cells ... We use FuGene-6 with HEK293 We use FuGene-6 with HEK293 cells pretty routinely and get good results. We just use the standard ... Home/ Forums/ Cell Culture and Tissue Culture: Transfection and Assay Development/ transfection of HEK293 cells. ...
Quantitative characterization of capsaicin-induced TRPV1 ion channel activation in HEK293 cells by impedance spectroscopy. ... A pharmacological cocktail for arresting actin dynamics in living cells. Mol Biol Cell. 2011;22(21):3986-94. doi: 10.1091/mbc. ... Annu Rev Cell Dev Biol. 2013;29:355-84. doi: 10.1146/annurev-cellbio-101011-155833.CrossRefGoogle Scholar ... Cell Calcium. 2005;38(3-4):233-52. doi: 10.1016/j.ceca.2005.06.028.CrossRefGoogle Scholar ...
Virus expression and transfection of stable cell lines. 293 SFM II. No. No. Serum-free growth medium for HEK 293 suspension ... Select the Gibco® serum-free HEK 293 growth medium that is right for your research:. ...
293 cells is an immortal cell line.. The 293 T cell is the same cell lineage, in which the large T antigen from virus SV40 ... Top : Forum Archives: : Cell Biology. new memeber:choose which ,hek293 or 293t? - (May/22/2006 ). hello everyone ,i am a new ... although i have looked up some papers i donot know which kind of 293 cells i should choose for my transfection ,hek293 or 293t ... Both of the 293 (HEK293 and 293T) should work perfectly fine for transfection. Stick to one of them for all your experiments. ...
... expressed in HEK 293 cells, ≥95% (SDS-PAGE); Synonym: PP5, REF1; find Sigma-Aldrich-SRP6486 MSDS, related peer-reviewed papers ... expressed in HEK 293 cells, ≥95% (SDS-PAGE) Synonym: PP5, REF1 ... and retinal pigment epithelial cell factor 1 (REF-1), is a ... TFPI2 is highly abundant in the full-term placenta and widely expressed in umbilical vein endothelial cells, liver, placenta, ...
Isotyp-Kontrollen und Produkte wie HEK-293 Cells IgG2 isotype control bestellen auf www.antikoerper-online.de. Jetzt Produkt ... HEK-293 Cells IgG2 Isotyp-Kontrolle HEK-293 Cells IgG2 Isotyp-Kontrolle. Details zu Produkt Nr. ABIN2181275, Anbieter: Anmelden ... Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting ...
Catalog Products » Other Products » Stable Cell Lines » Human Recombinant Angiotensin Receptor 1 Stable Cell Line ... Human Recombinant Angiotensin Receptor 1 Stable Cell Line Description. Angiotensin receptor 1 (AT1 receptor) is a member of ... vascular smooth muscle cells proliferation, decreased renal blood flow, renal renin inhibition, renal tubular sodium reuptake, ...
Home/ Forums/ Neuroscience: Assay Development & Protocols/ Endogenous channels in HEK293 cells. Endogenous channels in HEK293 ... Endogenous channels in HEK293 cells Could anybody suggest any literature survey on endogenous ion channels in the plasma ... Surprisingly, HEK-293 cells were found to contain mRNA for HCN2 and HCN3 although we have not detected a significant and ... A transformed line of human embryonic kidney epithelial cells (HEK 293) is commonly used as an expression system for exogenous ...
... simple binding and functional assays using irradiated cells transfected with human adenosine A2B receptor - no cell culture ... in HEK293 host cell. We provide one vial of frozen cells (2.5 million cells/vial). Some of our Frozen cells may be restricted ... Our frozen, irradiated cAMPZen cells express a variety of GPCRs for binding and functional assays. No cell culture is required ... Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you ...
... reporter gene into HEK293 cells.The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to ... hNOD2 cells were obtained by co-transfection of the human NOD2 gene and an optimized secreted embryonic alkaline phosphatase ( ... Human NOD2 / NF-kB / SEAP reporter HEK293 cells. HEK-Blue™-hNOD2 cells were obtained by co-transfection of the human NOD2 gene ... The parental cell line of HEK-Blue™ hNOD2 cells is HEK-Blue™ Null2 cells. ...
... and uptake by HEK293 epithelial cells using live confocal microscopy and flow cytometry. In media pH 7.4 with or without 10% ... Serum stimulated cells to acidify the media, partly by lactate generation. Media acidified to pH 6.5 by 7 mM lactate maintained ... and serum influence the rate of cell uptake. Chitosans with 80% and 95% degree of deacetylation (medium and low viscosity) were ... Keywords: chitosan; HEK293 cells; lactate; EGF; serum; confocal microscopy; microparticle chitosan; HEK293 cells; lactate; EGF ...
Gene Name P2RX3 Abbr HEK293-HuP2RX2/P2RX3 Alias P2RX3,P2X3,MGC129956 ... acroscell.creative-bioarray.com/human-p2rx2-p2rx3-stable-cell-line-hek293-acc-ri0184-item-146.htm. ...
Find and order Isotype Controls and products like HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag) on www.antibodies- ... HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag) HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag). Details ... Images for product: HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag) ... Images for product: HEK-293 Cells IgG isotype control (AVI tag,Biotin,His tag) ...
"Time-Dependent Effects of Anesthetic Isoflurane on Reactive Oxygen Species Levels in HEK-293 Cells." Brain Sciences 4 (2): 311- ... Time-Dependent Effects of Anesthetic Isoflurane on Reactive Oxygen Species Levels in HEK-293 Cells. ... The cells were treated with 2% isoflurane plus 21% O2 and 5% CO2 for 15, 30, 60, or 90 min. We then used fluorescence imaging ... performed a pilot study to determine the effects of treatment with isoflurane for various times on ROS levels in HEK-293 cells ...
... to compare the DNA methylation profile of HEK293 cells expressing low levels of BAHD1 (HEK-CT) to that of isogenic cells stably ... to compare the DNA methylation profile of HEK293 cells expressing low levels of BAHD1 (HEK-CT) to that of isogenic cells stably ... We identified 91,358 regions that have different methylation patterns in HEK-BAHD1 compared to HEK-CT cells (termed ... We identified 91,358 regions that have different methylation patterns in HEK-BAHD1 compared to HEK-CT cells (termed ...
Human - from HEK293+cells - Proteins. tag: His - Proteins. from HEK293+cells - tag: His - Proteins. from HEK293+cells - ... BMP-6 - from HEK293+cells - Proteins. Recombinant+protein - Proteins. Human - Recombinant+protein - Proteins. Recombinant+ ... Recombinant+protein - from HEK293+cells - Proteins. Human - Proteins. Human - tag: His - Proteins. ... You are here: Home Products and Services Proteins BMP-6 Human HEK293 cells ...
... exosomes isolated from HEK293 cells are carefully manufactured in SBIs Palo Alto facilities. Starting with cells grown in Exo- ... MDA-MB-231 Human breast cancer, aggressive/invasive/metastatic cell line. EXOP-115A-1. PC-3 Human prostate cancer cells derived ... Accelerate your exosome studies with these ready-to-use purified exosomes from HEK293 cells. ... Frozen exosomes (,1×10^10) from HEK293 Human embryonic kidney cell line ...
... inhibition of E217betaG uptake in membrane vesicles from MRP4-expressing HEK-293 cells. ...
It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system. ... HEK293-HuTRPC1 cell line is a hypotriploid human cell line, which has been transfected with a human transient receptor ... Cell Line Description:. HEK293-HuTRPC1 cell line is a hypotriploid human cell line, which has been transfected with a human ... Host Cell:. HEK293. Cell Line Validation:. 1. Gene expression: qPCR experiments determined specific expression of human TRPC1.2 ...
It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system. ... HEK293-HuHCN4 cell line is a hypotriploid human cell line, which has been transfected with a human hyperpolarization activated ... Cell Line Description:. HEK293-HuHCN4 cell line is a hypotriploid human cell line, which has been transfected with a human ... Host Cell:. HEK293. Cell Line Validation:. 1. Gene expression: qPCR experiments determined specific expression of human HCN4.2 ...
Antagonist activity in capsaicin-induced FLIPR assays in HEK293 cells expressing human TRPV1. ...
... a human hepatoma cell line HepG2, a human astrocytoma cell line 1321N1, and a human embryonic kidney cell HEK 293. The results ... Significant decreases in ATP production were also observed at all Cd concentrations in HepG2 and HEK 293 cell lines. ROS levels ... In this the present study, the toxic effect of 5, 10 and 50 μM of Cd chloride was compared in three human cell lines; ... The endogenous levels of protective enzymes as well as their responsiveness are likely to determine a cells susceptibility to ...
  • ACE2/HEK293 Stable Cell Line is a stably transfected HEK293 cell line which expresses human angiotensin-converting enzyme 2 (ACE2). (abeomics.com)
  • Right here we display that two unique PKA inhibitors drastically reduce the constitutive production of both A40 and A42 in stably transfected HEK293 cells expressing crazy type (wt) and Swedish mutated (Sw) APP751. (healthandwellnesssource.org)
  • Cell membranes prepared from HEK293 cells stably-transfected with human serotonin 5HT2c receptor, 400 units. (perkinelmer.co.uk)
  • Here, we report that BER could not only significantly decrease the production of beta-amyloid 40/42 and the expression of beta-secretase (BACE), but was also able to activate the extracellular signal-regulated kinase1/2 (ERK1/2) pathway in a dose- and time-dependent manner in HEK293 cells stably transfected with APP695 containing the Swedish mutation. (biomedcentral.com)
  • In this study, we therefore focused on the mechanism of BER on BACE and Aβ 40/42 inhibition, using HEK293 cells stably transfected with APP695 containing the Swedish mutation. (biomedcentral.com)
  • Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture taken from a female fetus. (wikipedia.org)
  • HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
  • Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
  • A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue. (wikipedia.org)
  • Given the location of the adrenal gland (adrenal means "next to the kidney"), a few adrenal cells could plausibly have appeared in an embryonic kidney derived culture, and could be preferentially transformed by adenovirus. (wikipedia.org)
  • Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. (wikipedia.org)
  • As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. (wikipedia.org)
  • Cell line: HEK293 (Human embryonic kidney cell line). (abcam.com)
  • Human Embryonic Kidney (HEK) 293 cells were originally derived from human embryonic kidney and subsequently demonstrated to be a useful cell type to produce adenovirus, other viral vectors, and effectively glycosylated human recombinant proteins. (abcam.com)
  • The positive control is HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902). (abcam.com)
  • 293-Dual™ STING (ISG/KI-IFNb) cells were generated from 293-Dual™ Null (ISG/KI-IFNb) cells, which derive from 293 cells, human embryonic kidney 293-derived cells. (invivogen.com)
  • Human Embryonic Kidney 293 cells, also known as HEK293, 293 cells, or even as HEK cells, are a cell line derived from human embryonic kidney. (moleculardevices.com)
  • We manufacture a variety of Gibco® serum-free media formulations that are designed to maximize the growth and expansion of suspension human embryonic kidney (HEK) 293 cells. (thermofisher.com)
  • A transformed line of human embryonic kidney epithelial cells (HEK 293) is commonly used as an expression system for exogenous ion channel genes. (scientistsolutions.com)
  • a human hepatoma cell line HepG2, a human astrocytoma cell line 1321N1, and a human embryonic kidney cell HEK 293. (go.jp)
  • As an initial approach to address whether TWEAK might act as a tumor angiogenesis factor, we established several human embryonic kidney cell lines that constitutively secrete a soluble TWEAK protein and examined their growth properties in vitro and in vivo . (aacrjournals.org)
  • In the present study we used voltage-clamp electrophysiology to characterize excitatory glycine-activated receptors assembled from NR1, NR3A, and NR3B subunits expressed in human embryonic kidney (HEK) 293 cells. (aspetjournals.org)
  • subunits modulate the kinetics of channel opening as well as increase the number of functional channels expressed in heterologous expression systems such as Xenopus oocytes and human embryonic kidney cells (HEK-293). (asahq.org)
  • To clarify the function of CNNM2, Human Embryonic Kidney (HEK293) cells were transiently transfected with mouse Cnnm2 or mock constructs and examined for Mg2+ transport capacity using the stable 25Mg2+ isotope. (nih.gov)
  • I was particularly fascinated that he actually speculated that these cells may have originated from a rare neuronal cell in the kidney cell cultures, since at about the same time but completely independently we had come to a similar conclusion ( link to *.pdf here - Dr. Van der Eb's part starts on page 77, and the specific comment about the potential neuronal origin of 293 cells is on page 85). (novoprolabs.com)
  • So I reached into the fridge for a monoclonal antibody which would have no business staining a kidney cell, and since one of the things I work on is neurofilaments, I got out one of our monoclonals to NF-M, one of the major neurofilament subunits. (novoprolabs.com)
  • Further studies revealed that several other cell lines derived from human embryonic kidney cultures by adenovirus transformation also express neurofilaments, as did adenovirus derived cell lines from human embryonic retina, and the basic pattern of neurofilament expression was similar to that of 293 cells. (novoprolabs.com)
  • Adenovirus‐transformed human embryonic kidney cell clonal line. (ch3biosystems.com)
  • ACE2 is a type I transmembrane metalloenzyme located on the outer surface of endothelial cells in the lung, arteries, heart, kidney and intestines. (abeomics.com)
  • We previously established that the forming of both - and /-secretase-derived items generated by human being embryonic kidney 293 cells (HEK293) expressing either crazy type or mutant APP could possibly be stimulated by agonists from the cyclic AMP/proteins kinase A pathways. (healthandwellnesssource.org)
  • In order to elucidate a putative direct cytotoxic action of Echis carinatus venom we used an in vitro system, using cultured Human embryonic kidney (HEK 293) cells monolayers as a model. (clinmedjournals.org)
  • Here we show that GA reduces the level of endogenous c-Jun in human embryonic kidney 293 (HEK293) cells in a time and dose dependent manner, and that this decrease can be reversed by transfection of HSP90 plasmids. (koreascience.or.kr)
  • In this study transient expression of scFv-Fc antibodies in human embryonic kidney (HEK) 293 cells was optimized. (biomedcentral.com)
  • In 1972 van der Eb obtained and managed to culture embryonic kidney cells from a legally aborted foetus. (wikipedia.org)
  • Examples of such experiments include: Effects of a drug on sodium channels Inducible RNA interference system Isoform-selective protein kinase C agonist Interaction between two proteins Nuclear export signal in a protein HEK 293 cells were adapted to grow in suspension culture, as opposed to proliferation on plastic plates, in 1985. (wikipedia.org)
  • We began with existing commercial media and supplemented them with specific proteins to improve cell growth. (bioprocessintl.com)
  • This fluid is rich in proteins and cells, such as: cancer cells, lymphocytes and mesothelial cells. (termedia.pl)
  • dSTORM in hypotonic cells revealed a surface density of membrane-associated SLC5A3 proteins of 200-2000 localizations/μm2. (uni-wuerzburg.de)
  • In contrast, cells expressing mutated CNNM2 proteins did not show increased Mg(2+) uptake. (nih.gov)
  • hTRPC3_HEK293 Recombinant Cell line recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. (antibody-antibodies.com)
  • Cell signalling ensues in the absence of transmembrane peptidoglycan recognition proteins and the adaptor molecules Fas-associated protein with a death domain (FADD) and IMD. (nature.com)
  • This analysis was stimulated by the observation that 293 cells stain strongly and specifically with antibodies to several neurofilament proteins, which are generally thought of as excellent markers for neuronal lineage cells. (novoprolabs.com)
  • Effectors from the proteins kinase C have already been shown to reduce A creation and boost secretion from the -secretase-derived physiological item APP in a variety of cell lines (Caporaso et al . (healthandwellnesssource.org)
  • 1998). We lately showed how the maturation of APP is apparently under control from the proteins kinase A (PKA) pathway 152658-17-8 manufacture in human being cells and neurons overexpressing regular and FAD-linked APP (Marambaud et al . (healthandwellnesssource.org)
  • These small molecular weight Ca2+-binding proteins typically increase cell surface expression of the channel complex, accelerate the rate of decay of the current at depolarized potentials, and increase the rate of recovery from inactivation at hyperpolarized potentials, i.e. the kinetics then more closely resembles native Ito than if Kv4.3 subunits were expressed alone. (creative-bioarray.com)
  • Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. (sinobiological.com)
  • The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. (scilifelab.se)
  • Leave the T25 flask in the incubator for 1~2 days without disturbing or changing the medium until cells completely recover viability and become adherent. (abeomics.com)
  • Once cells are over 90% adherent, remove growth medium and passage the cells through trypsinization and centrifugation. (abeomics.com)
  • Production levels of 10-20 mg/L scFv-Fc antibody were achieved in adherent HEK293T cells. (biomedcentral.com)
  • Evolution from adherent to suspension: systems biology of HEK293 cell line development. (scilifelab.se)
  • Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin. (scilifelab.se)
  • This H5N1 Hemagglutinin/HA overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. (sinobiological.com)
  • Recombinant Sus scrofa (pig) C-C motif Chemokine 2 (MCP-1) Protein, Transfected HEK293 Cell Lysate by RayBiotech, Cat. (lucerna-chem.ch)
  • Providing our customers with innovative bioanalytical solutions for protein and cell biology for over 30 years. (moleculardevices.com)
  • HEK293 cells are easy to grow and transfect, so they have been used extensively in transfection-based experiments, protein expression, and vaccine production. (moleculardevices.com)
  • The 293 T cell is the same cell lineage, in which the large T antigen from virus SV40 which stabilizes the p53 protein. (protocol-online.org)
  • Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). (sigmaaldrich.com)
  • BAH domain-containing protein 1 (BAHD1) is involved in heterochromatin formation and gene repression in human cells. (frontiersin.org)
  • Aliquots of purified exosomes from the cell lines and from human serum were lysed with either RIPA or M-PER buffer to make exosome protein lysates. (systembio.com)
  • Protein expression: TRPC1 expression in this cell line has been validated by WB.3. (creativebiomart.net)
  • Protein expression: HCN4 expression in this cell line has been validated by WB.3. (creativebiomart.net)
  • Therefore, we propose to use insulin, a protein currently used and approved in several processes and production platforms, as an additive to boost cell growth and viral production. (bioprocessintl.com)
  • In addition, heterologous protein expression used small cells from mammalian cell lines that provided for high resolution analysis of current time courses. (asahq.org)
  • The use of HEK293 cells overexpressing OATP1B1 and OATP1B3 in protein-free buffer and 100% human plasma incubations was explored for the uptake of four OATP substrates, pravastatin, rosuvastatin, repaglinide and pitavastatin. (aspetjournals.org)
  • Differences were observed for each parameter (Km,u, Vmax, CLint, and Pdif,u) obtained from the buffer and human plasma incubations in both cells, and in general, the fold differences increased as plasma protein binding increased. (aspetjournals.org)
  • As a result, the CLint values generated in the plasma incubations were 1.66-51.9 fold higher than the values generated in protein-free buffer in both cells. (aspetjournals.org)
  • The present study explores the SLC5A3 protein as a possible transporter of myo-inositol in hyponically swollen HEK293 cells. (uni-wuerzburg.de)
  • Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. (sinobiological.com)
  • The propensity of syntaxin1A to promote voltage-dependent steady-state inactivation of N-type Ca(2+) channels and accelerate their entry into inactivation was used in a heterologous cell expression system to elucidate regulation of syntaxin1A protein-protein interactions. (nih.gov)
  • Furthermore, Aβ-treated cells displayed a microtubule-associated protein 1 light chain 3 (LC3) punctate pattern and elevated mitochondrial LC3-II levels, suggesting the upregulation of mitophagy. (usda.gov)
  • Etoposide (ETP) treatment of ataxia telangiectasia mutated (ATM) and Rad3-related protein (ATR)-, topoisomerase-binding protein-1 (TopBP1) and human MutY homolog (hMYH)-depleted cells results in a significant reduction in apoptotic signaling. (semanticscholar.org)
  • This study outlines the comprehensive mRNA and protein analysis of 293 and 293T cells in the context of LECs using standard and real-time RT-PCR and flow cytometry analysis. (pitt.edu)
  • L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. (bpsbioscience.com)
  • Fig-1: Binding of biotinylated SARS-Cov-2 Spike RBD protein to human ACE2 in the ACE2/HEK293 stable cell line. (abeomics.com)
  • ACE2/HEK293 cells were probed with different amounts of biotinylated SARS-Cov-2 Spike RBD protein (Abeomics, Cat. (abeomics.com)
  • Stable recombinant HEK293 cell line expressing human Nav1.7 (Genbank # Q15858) fused at the C-terminus to Green Fluorescent Protein [Ex. (bpsbioscience.com)
  • the proteasome inhibitor N-benzoyloxycarbonyl (Z)-Leu-Leu-leucinal (MG132) blocks the degradation of c-Jun promoted by GA. Transfection of HSP90 plasmids did not obviously alter phosphorylation of c-Jun, and a Jun-2 luciferase activity assay indicated that over-expression of HSP90 elevated the total protein activity of c-Jun in HEK293 cells. (koreascience.or.kr)
  • All our evidence indicates that HSP90 stabilizes c-Jun protein, and so increases the total activity of c-Jun in HEK293 cells. (koreascience.or.kr)
  • Vinculin (VCL) is a cytoskeletal protein that is closely related to both cell-matrix interactions and cell-cell junctions. (sinobiological.com)
  • 2001) Leukotriene D(4) affects localisation of vinculin in intestinal epithelial cells via distinct tyrosine kinase and protein kinase C controlled events. (sinobiological.com)
  • Chitosans with 80% and 95% degree of deacetylation (medium and low viscosity) were tagged with rhodamine and analyzed for particle size, media solubility, and uptake by HEK293 epithelial cells using live confocal microscopy and flow cytometry. (mdpi.com)
  • Accelerate your exosome studies with these ready-to-use purified exosomes from HEK293 cells. (systembio.com)
  • Hi Ivan i have a question regarding confluency of HEK293 cells for transfection.Yous uggested to thaw a new vial of cells if the cells had reached confluency( even if it was few passages before). (scientistsolutions.com)
  • We provide one vial of frozen cells (2.5 million cells/vial). (perkinelmer.com)
  • Remove the cells from the vial and add slowly into a 15ml conical tube containing 10 ml pre-warmed media. (creativebiomart.net)
  • Including one vial of HEK293 cell lysate transfected with empty expression vector. (raybiotech.com)
  • The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. (raybiotech.com)
  • Also is there a difference with vendors (ie, some have better cell lines? (protocol-online.org)
  • Hey, I don`t think there would be a big difference between those two cell lines regarding transfection. (protocol-online.org)
  • Or probably more importantly, what if the cell lines stored in our liquid nitrogen had once became confluent. (scientistsolutions.com)
  • 1. Remove the packaging cell lines from liquid nitrogen and carry out a quick thaw. (creativebiomart.net)
  • The results indicate a decrease in the viability of all three cell lines following exposure to Cd with HepG2 cells (IC 50 = 13.96 μM) showing the most sensitivity when measured using the MTT assay. (go.jp)
  • There was significant increase in lactate dehydrogenase leakage, DNA damage, malondialdeyde and antioxidant enzymes activities in all three cell lines especially at 50 μM Cd. (go.jp)
  • Significant decreases in ATP production were also observed at all Cd concentrations in HepG2 and HEK 293 cell lines. (go.jp)
  • ROS levels significantly increase and GSH/GSSG ratio significantly decrease in all the three cell lines after Cd exposure, but these effects were attenuated by the presence of N-acetylcysteine (NAC). (go.jp)
  • The present study therefore shows that ROS production and glutathione (GSH) depletion may play a role in Cd-induced toxicity in all the three cell lines. (go.jp)
  • Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)
  • The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines. (amsbio.com)
  • Compared to vehicle, clofibric acid, benoxaprofen, and probenecid produced significant DNA damage in all three UGT-transfected HEK293 cell lines, detectable from the lowest concentration tested. (nih.gov)
  • Supplementation of commercial media with specific compounds was studied using a design of experiments (DoE) approach to screen the most efficient compounds for two cell lines and then determine their optimal conditions. (bioprocessintl.com)
  • We used these approaches systematically with two different cell lines: HEK293 and Chinese hamster ovary (CHO) cells. (bioprocessintl.com)
  • Two types of cells used within cell culture include primary and established cell lines. (pitt.edu)
  • Furthermore, this study evaluates the influence of culture conditions on LEC marker expression by 293 and 293T cells in an attempt to find optimal LEC growth conditions for these cell lines. (pitt.edu)
  • So the 293 cells were similar in several respects to early differentiating neurons and to two well defined neuronal lineage cell lines. (novoprolabs.com)
  • or (4) resale of the cell line whether or not such cell lines are resold for use in research. (abeomics.com)
  • In addition, we thoroughly test our cell lines for freeze-thaw recovery. (abmgood.com)
  • 7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. (abmgood.com)
  • CRISPR-U™ has been successfully worked with more than 100 types of cell lines . (ubigene.us)
  • It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. (sinobiological.com)
  • Extracellular K+ concentration controls cell surface density of IKr in rabbit hearts and of the HERG channel in human cell lines. (brad.ac.uk)
  • Several cell lines undergo massive mitochondrial damage and apoptosis upon acute intracellular GD3 accumulation, a condition which can be experimentally achieved by GD3 synthase overexpression. (nih.gov)
  • Selected cell lines, however, while still sensitive to exogenous GD3 exposure, fail to undergo apoptosis upon GD3 synthase overexpression and endogenous GD3 accumulation. (nih.gov)
  • Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. (scilifelab.se)
  • Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. (scilifelab.se)
  • He has performed research in adenoviruses and was fundamental in the creation of the technique of calcium phosphate transfection and the founding of the HEK 293 and PER.C6 cell lines. (wikipedia.org)
  • HEK 293 became one of the most used cell lines worldwide, coming close to the use of the HeLa cell line. (wikipedia.org)
  • citation needed] HEK 293F HEK 293FT HEK 293T HEK 293S HEK 293FTM HEK 293SG HEK 293SGGD HEK 293H HEK 293E HEK EBNA1-6E HEK 293MSR HEK 293A HEK 293 cells are straightforward to grow in culture and to transfect. (wikipedia.org)
  • Are you sure that your cells are nice plated and looking happy when you transfect? (protocol-online.org)
  • I have been trying to transfect HEK293 cells with Transfast and Lipofectamine and have not been able to confirm successful transfection. (scientistsolutions.com)
  • Simply detach healthy log phase cells, count and plate at high density and transfect with GP3K an your plasmid of choice. (scientistsolutions.com)
  • You can definitely transfect cells that have reached 100% confluency, that is perfectly fine. (scientistsolutions.com)
  • Retrieving chromosomal sequences flanking the transposon selleck Z-VAD-FMK targets by plasmid rescue The identical transfection procedure comprehensive previously was utilized to transfect the piggyBac donor, pXLBacII cassette, and Tol2 donor, Tol2ends cassette, coupled with their cor responding helper, pPRIG piggyBac and pPRIG Tol2, respectively, into HEK 293 cells employing Fugene HD. (phosphorylase-signal.com)
  • who would have thought that you would only have to mix DNA with a bit of calcium phosphate to get the DNA incorporated and even expressed in mammalian cells? (novoprolabs.com)
  • The purpose of this study was to test the hypothesis that chitosan structure, solubility state, and serum influence the rate of cell uptake. (mdpi.com)
  • CNNM2 increases Mg2+ uptake in HEK293 cells. (nih.gov)
  • Using stable Mg(2+) isotopes, we demonstrated that CNNM2 increases cellular Mg2+ uptake in HEK293 cells and that this process occurs through regulation of the Mg(2+)-permeable cation channel TRPM7. (nih.gov)
  • A) Time curve of 25Mg2+ uptake in mock (circles) and CNNM2 (squares) transfected cells. (nih.gov)
  • C) 25Mg2+ uptake in the presence of inhibitors of ion transporters, black bars represent mock cells and white bars represent CNNM2-transfected cells. (nih.gov)
  • E) The effect of Na+ and Cl− availability on 25Mg2+ uptake in mock (black bars) and CNNM2 (white bars) transfected cells. (nih.gov)
  • F) 25Mg2+ uptake as a function of extracellular 25Mg2+ availability in mock (circles) and CNNM2 (squares) transfected cells. (nih.gov)
  • The effect of Echis Carinatus snake venom on HEK 293 cells viability was determined by MTT assay and neutral red uptake assay. (clinmedjournals.org)
  • KCC2 transfected cells displayed a significant 1.5 fold increase in 86 Rb + uptake compared to mock-transfected control cells (Figure 1a , left part), when preincubated at 37°C prior flux measurements. (biomedcentral.com)
  • It might not feasible to find a 293T cell line in a lab that might not have become 100% confluent at least once during the passages. (scientistsolutions.com)
  • new memeber:choose which ,hek293 or 293t? (protocol-online.org)
  • Both of the 293 (HEK293 and 293T) should work perfectly fine for transfection. (protocol-online.org)
  • Just be careful that transfected plasmids may replicate in 293t because they have a SV40 promoter (typically contains a functional SV40 replication origin) and that may give you false 'amplification' results that you will not get in other cell types. (protocol-online.org)
  • Among established cells HEK 293 and 293T cells are among the most widely used in the technique of cell culture. (pitt.edu)
  • Previous studies have characterized 293 and 293T cells as neuronal in origin although phenotypic analyses have not been reported. (pitt.edu)
  • Similarly, studies dealing with the binding characteristics of HIV have revealed the expression of podoplanin, a typical LEC marker, by 293T cells. (pitt.edu)
  • These observations of podoplanin expression suggest that the phenotype of 293 and 293T cells needs to be further explored and that they might resemble LECs. (pitt.edu)
  • Evaluation of mRNA expression levels revealed that 293 and 293T cells express multiple LEC markers including Prox-1, Lyve-1, PDPN, and VEGFR-3. (pitt.edu)
  • Studies investigating the manipulation of culture conditions revealed that 293 and 293T cells do not significantly change in LEC marker expression. (pitt.edu)
  • Taken together, these studies suggest that 293 and 293T cells phenotypically resemble LECs and should be defined as LEC-like. (pitt.edu)
  • Defining 293 and 293T cells as LECs is highly relevant to the field of public health as it provides a new model by which to study LEC function in several different contexts including vaccine interaction and cancer metastasis. (pitt.edu)
  • TFPI2 is highly abundant in the full-term placenta and widely expressed in umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. (sigmaaldrich.com)
  • Lymphatic endothelial cells (LECs) are often represented as primary cells, which have been isolated from tissues. (pitt.edu)
  • Morphological changes of endothelial cells were also evaluated using a phase contrast microscope. (clinmedjournals.org)
  • HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. (wikipedia.org)
  • Graham performed the transfection a total of eight times, obtaining just one clone of cells that were cultured for several months. (wikipedia.org)
  • I was wondering if my transfections are not working because I'm using the wrong cell line, I have been using HEK293, but is HEK293T better for transient and stable transfection? (protocol-online.org)
  • If this is the case, you may simply want to thaw a new tube and start your cells again because you will likely come across the same problem with other transfection reagents. (scientistsolutions.com)
  • Does it mean that once HEK293 cells reached 100% confluency, it CANNOT be used at all for transfection expts? (scientistsolutions.com)
  • My point was that if you use cells that have reached 100% confluency your transfection efficiency will not be as good as when you use cells that have not reached 100% confluency. (scientistsolutions.com)
  • HEK-Blue™-NOD2 cells were obtained by co-transfection of the human or murine NOD2 gene and an optimized secreted embryonic alkaline phosphatase (SEAP) reporter gene into HEK293 cells. (invivogen.com)
  • However, as reported in the present study, simultaneous transfection of cDNAs encoding NR1, NR3A, and NR3B subunits into HEK 293 cells produces robust glycine-activated currents. (aspetjournals.org)
  • For HEK293 cells producing Gag-GFP virus-like particles (VLPs), we used polyethylenimine transient transfection. (bioprocessintl.com)
  • The best options for cell growth are not necessarily the best for transient transfection. (bioprocessintl.com)
  • The optimal media for cell growth are not best for transient transfection, and vice versa. (bioprocessintl.com)
  • Three of the four we tested had a more positive effect on cell growth and transient transfection, and we retained those. (bioprocessintl.com)
  • B) GD3 and 9-O-acetyl GD3 content of HEK-293 cells was analyzed by TLC and immunostaining 40 h after transfection with the indicated constructs. (nih.gov)
  • Temperature-dependence of KCC2 and KCC4 transport activity in HEK-293 cells was determined after transient transfection by 86 Rb + flux measurements. (biomedcentral.com)
  • Twenty 4 hours just after transfection, one fifth of transfected cells were subjected to transposition assay. (phosphorylase-signal.com)
  • With Graham's contributions in transfection of the cells with the adenovirus 5 this led to the cell line of HEK 293 cells. (wikipedia.org)
  • Whether these species induce comparable genetic damage in human cells is unknown. (nih.gov)
  • The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species. (bpsbioscience.com)
  • However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. (wikipedia.org)
  • In the early 1970's, Alex van der Eb isolated these cells in his laboratory at the University of Leiden, Holland, and another member of his lab transformed them by adenovirus, giving rise to the cell line used today. (moleculardevices.com)
  • A more specific use of HEK 293 cells is in the propagation of adenoviral vectors. (wikipedia.org)
  • HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. (raybiotech.com)
  • Transient production of recombinant scFv-Fc antibodies in HEK293-6E in combination with optimized vectors and fed batch shake flasks cultivation is efficient and robust, and integrates well into a high-throughput recombinant antibody generation pipeline. (biomedcentral.com)
  • 293-Dual™ STING and their parental cell line stably express an ISRE-inducible SEAP (secreted embryonic alkaline phosphatase) reporter construct. (invivogen.com)
  • Methods: HEK293 Cell line stably expressing hERG channel were cultured in DMEM supplemented with 10% of fetal bovine serum.Whole-cell patch-clamp method was applied for ionic current recordings. (brad.ac.uk)
  • I was making monoclonal antibodies to the beta-adrenergic receptor kinase 1 (beta-ARK), which is supposed to be endogenously heavily expressed in 293 cells. (novoprolabs.com)
  • The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control. (raybiotech.com)
  • Today, almost all therapeutic antibodies are produced in mammalian cells because their advanced folding, secretion and post-translational apparatus is best suited to produce antibodies indistinguishable from those produced in the human body with least concerns for immunogenic modifications. (biomedcentral.com)
  • TGF-β family members are key modulators of cell proliferation, differentiation, matrix synthesis and apoptosis. (biovendor.com)
  • Recent studies have demonstrated that TWEAK can stimulate numerous cellular responses including cell proliferation, migration, and proinflammatory molecule production. (aacrjournals.org)
  • Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. (sinobiological.com)
  • Starting with cells grown in Exo-FBS (our exosome-depleted FBS) to maximize purity, exosomes are isolated using ExoQuick-TC® and then quality checked by Western blot for the presence of CD63, and NanoSight for particle size and intactness. (systembio.com)
  • Could anybody suggest any literature survey on endogenous ion channels in the plasma membrane of HEK cells? (scientistsolutions.com)
  • The increase in Pino resulted most likely from the hypotonicity-mediated incorporation of cytosolic SLC5A3 into the plasma membrane, as revealed by confocal fluorescence microscopy of cells expressing EGFP-tagged SLC5A3 and super-resolution imaging of immunostained SLC5A3 by direct stochastic optical reconstruction microscopy (dSTORM). (uni-wuerzburg.de)
  • Membrane Target Systems: Serotonin 5HT2c (human) membrane preparation, in HEK-293 cells. (perkinelmer.co.uk)
  • Membrane Target Systems are quality assured frozen membranes from cells that express recombinant or endogenous receptors. (perkinelmer.co.uk)
  • The integrity of cell membrane through LDH release was measured with the LDH Kit. (clinmedjournals.org)
  • Crude venom caused changes in the integrity of cell membrane determined by rise in LDH release too. (clinmedjournals.org)
  • nAChR are transiently expressed in HEK293 cells membrane . (bvsalud.org)
  • Additionally, our lab has observed the expression of podoplanin by 293 cells through flow cytometry analysis. (pitt.edu)
  • The HEK293SF-3F6 cell line is a GMP cell line that has been adapted to growth in suspension in serum-free condition. (bioprocessintl.com)
  • To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. (abeomics.com)
  • Employment of HEK293-6E suspension cells expressing a truncated variant of the Epstein Barr virus (EBV) nuclear antigen (EBNA) 1 in combination with production under serum free conditions increased the volumetric yield up to 10-fold to more than 140 mg/L scFv-Fc antibody. (biomedcentral.com)
  • Some of the previous studies demonstrated the ability of ascites to modulate the gene expression, which is associated with changes in the cellular behavior of ovarian cancer cell [5, 6]. (termedia.pl)
  • Moreover the Rebecca D Pierce (2011) used polyethyleneimine (PEI) to enhance cellular adherence, and to determine whether the substrate attachment influenced the survival of cells treated with crude E. carinatus venom. (clinmedjournals.org)
  • The difficulty of targeting dyes to specific cell types presents a challenge for cellular-resolution voltage imaging in vivo . (frontiersin.org)
  • GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. (sinobiological.com)
  • 20E2 cells (HEK293-APPswe cells) are a cellular model of AD. (europeanreview.org)
  • The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer). (bioon.com.cn)
  • D) Dose-response curve of 25Mg2+ transport inhibition by 2-APB in mock (circles) and CNNM2 (squares) transfected cells. (nih.gov)
  • We show that both non-transfected HEK cells and HEK cells transfected with hyperpolarization-activated cyclic-nucleotide gated (HCN) channels have a significant amount of voltage-gated potassium (K(V)) current when certain tail current voltage-clamp protocols are used to assay HCN current activation. (scientistsolutions.com)
  • In MTT assay, crude venom induced dose dependent cytotoxic effects on HEK 293 cells which were confirmed by neutral red assay. (clinmedjournals.org)
  • See cell line data sheet for detailed culturing and assay protocol. (bpsbioscience.com)
  • Standard Luciferase Assay System for mammalian cells should be followed according to Assay kit manufacturer protocol. (bonopusbio.com)
  • Cell culture and transposition assay HEK 293 cells had been maintained in MEMa medium supplemented with 10% FBS, 100 units ml penicillin, and a hundred ug mL streptomycin. (phosphorylase-signal.com)
  • Nevertheless, it is likely that other tumor-derived angiogenic factors also function as key regulators of tumor neovascularization, and the identification and characterization of these factors, their cell surface receptors, and their intracellular signaling pathways may result in the development of additional cancer drugs. (aacrjournals.org)
  • This increase in production was accompanied by an activation of signaling pathways associated with cell survival. (bioprocessintl.com)
  • Swelling-activated pathways for myo-inositol, one of the most abundant organic osmolytes in mammalian cells, have not yet been identified. (uni-wuerzburg.de)
  • Pino values were determined by cell volumetry over a wide tonicity range (100-275 mOsm) in Swelling-activated pathways for myo-inositol, one of the most abundant organic osmolytes in mammalian cells, have not yet been identified. (uni-wuerzburg.de)
  • It forms cell surface-associated, non-covalent, disulfide-linked homodimers, and can bind and activate both VEGFR-2 (flk1) and VEGFR-3 (flt4) receptors. (biovendor.com)
  • The results of this study indicate that coexpression of NR1, NR3A, and NR3B subunits in HEK 293 cells results in glycineactivated receptors with novel functional and pharmacological properties. (aspetjournals.org)
  • Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. (sinobiological.com)
  • Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. (sinobiological.com)
  • Aim Expressing muscle acetylcholin e receptors in mammallian cells using receptor clone technique. (bvsalud.org)
  • The license does not permit use of this cell line in humans or for therapeutic or drug use. (bpsbioscience.com)
  • LinkLight™ HEK293 PTH1R GPCR/14-3-3 Cell Line is a stable monoclonal cell line in HEK293 cells, targeting Human PTH1R pathway via 14-3-3. (bonopusbio.com)
  • 293 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). (abcam.com)
  • Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). (sinobiological.com)
  • HEK293-HuTRPC1 cell line is a hypotriploid human cell line, which has been transfected with a human transient receptor potential cation channel, subfamily C, member 1 (TRPC1) to allow stably express of the human TRPC1. (creativebiomart.net)
  • In summary, we developed a quantitative impedimetric monitoring system for the analysis of TRPV1 channel activity as well as downstream-induced biological activity in living cells. (springer.com)
  • Viruses offer an efficient means of delivering genes into cells, which they evolved to do, and are thus of great use as experimental tools. (wikipedia.org)
  • In this work, we show that endogenous voltage-gated potassium channels in HEK cells are a significant source of outward current at positive potentials. (scientistsolutions.com)
  • Surprisingly, HEK-293 cells were found to contain mRNA for HCN2 and HCN3 although we have not detected a significant and consistent endogenous I(f)-like current in these cells. (scientistsolutions.com)
  • HEK-293 cells are resistant to endogenous GD3 accumulation and synthesize 9-O-acetyl GD3. (nih.gov)
  • The bile acid lithocolic acid acts as an agonist on the GPBA GPCR, also known as GPR131 or TGR5, to increase intracellular cAMP in cells that express the receptor. (foreign-trade.com)
  • Our data show that PKA inhibitors selectively impact the /-secretase pathway in human being cells and are potential pharmacological which may be able to reduce A formation in both sporadic and FAD-linked Alzheimer's disease. (healthandwellnesssource.org)
  • Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells. (semanticscholar.org)
  • As shown in Fig. 3 A, HEK-293 cells, which undergo apoptosis upon exposure to exogenous GD3 (Fig. 2 B), are completely resistant to GD3 synthase overexpression. (nih.gov)
  • Frozen Cells validated for cAMP testing: Adenosine A2B receptor, human recombinant, in HEK293 host cell. (perkinelmer.com)