Hek293 cells. Medical search

A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.

Established cell cultures that have the potential to propagate indefinitely.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.

An eph family receptor that is found primarily in adult BRAIN and variety of tissues in the developing embryo tissues. During embryonic development high levels of EphA3 receptor expression is seen in the nervous system and coincides with neuronal cell migration, suggesting a role for this protein in axonal pathfinding.

An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Proteins prepared by recombinant DNA technology.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

The opening and closing of ion channels due to a stimulus. The stimulus can be a change in membrane potential (voltage-gated), drugs or chemical transmitters (ligand-gated), or a mechanical deformation. Gating is thought to involve conformational changes of the ion channel which alters selective permeability.

A subgroup of TRP cation channels that contain 3-4 ANKYRIN REPEAT DOMAINS and a conserved C-terminal domain. Members are highly expressed in the CENTRAL NERVOUS SYSTEM. Selectivity for calcium over sodium ranges from 0.5 to 10.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).

Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution.

Regulatory proteins that down-regulate phosphorylated G-protein membrane receptors, including rod and cone photoreceptors and adrenergic receptors.

The relationship between the dose of an administered drug and the response of the organism to the drug.

A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.

Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.

The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.

The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.

The rate dynamics in chemical or physical systems.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).

A family of voltage-gated potassium channels that are characterized by long N-terminal and C-terminal intracellular tails. They are named from the Drosophila protein whose mutation causes abnormal leg shaking under ether anesthesia. Their activation kinetics are dependent on extracellular MAGNESIUM and PROTON concentration.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.

An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.

Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.

Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.

A subgroup of TRP cation channels named after vanilloid receptor. They are very sensitive to TEMPERATURE and hot spicy food and CAPSAICIN. They have the TRP domain and ANKYRIN repeats. Selectivity for CALCIUM over SODIUM ranges from 3 to 100 fold.

A purinergic P2X neurotransmitter receptor that plays a role in pain sensation signaling and regulation of inflammatory processes.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.

Potassium channels where the flow of K+ ions into the cell is greater than the outward flow.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)

Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.

A family of proteins involved in the transport of organic cations. They play an important role in the elimination of a variety of endogenous substances, xenobiotics, and their metabolites from the body.

A class of G-protein-coupled receptors that react to varying extracellular CALCIUM levels. Calcium-sensing receptors in the PARATHYROID GLANDS play an important role in the maintenance of calcium HOMEOSTASIS by regulating the release of PARATHYROID HORMONE. They differ from INTRACELLULAR CALCIUM-SENSING PROTEINS which sense intracellular calcium levels.

Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.

Cell membrane glycoproteins that are selectively permeable to potassium ions. At least eight major groups of K channels exist and they are made up of dozens of different subunits.

Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.

Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

Ion channels that specifically allow the passage of SODIUM ions. A variety of specific sodium channel subtypes are involved in serving specialized functions such as neuronal signaling, CARDIAC MUSCLE contraction, and KIDNEY function.

A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.

An eph family receptor found in a number of tissues including BRAIN; LUNG; KIDNEY; PANCREAS; INTESTINE; and HEART. During embryogenesis EphB3 receptor is expressed at high levels in the brain.

The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)

A subclass of serotonin receptors that form cation channels and mediate signal transduction by depolarizing the cell membrane. The cation channels are formed from 5 receptor subunits. When stimulated the receptors allow the selective passage of SODIUM; POTASSIUM; and CALCIUM.

A subgroup of TRP cation channels named after melastatin protein. They have the TRP domain but lack ANKYRIN repeats. Enzyme domains in the C-terminus leads to them being called chanzymes.

A voltage-gated sodium channel subtype that mediates the sodium ion PERMEABILITY of CARDIOMYOCYTES. Defects in the SCN5A gene, which codes for the alpha subunit of this sodium channel, are associated with a variety of CARDIAC DISEASES that result from loss of sodium channel function.

Incorporation of biotinyl groups into molecules.

Potassium channel whose permeability to ions is extremely sensitive to the transmembrane potential difference. The opening of these channels is induced by the membrane depolarization of the ACTION POTENTIAL.

A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.

The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.

A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.

A family of heterotrimeric GTP-binding protein alpha subunits that activate TYPE C PHOSPHOLIPASES dependent signaling pathways. The Gq-G11 part of the name is also spelled Gq/G11.

A secreted protein that associates with TOLL-LIKE RECEPTOR 4 and is essential for receptor recognition of LIPOPOLYSACCHARIDES.

CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.

Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.

Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.

A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed)

A delayed rectifier subtype of shaker potassium channels that conducts a delayed rectifier current. It contributes to ACTION POTENTIAL repolarization of MYOCYTES in HEART ATRIA.

A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.

A pattern recognition receptor that forms heterodimers with other TOLL-LIKE RECEPTORS. It interacts with multiple ligands including PEPTIDOGLYCAN, bacterial LIPOPROTEINS, lipoarabinomannan, and a variety of PORINS.

Transport proteins that carry specific substances in the blood or across cell membranes.

The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.

A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.

Mammalian Notum induces the release of glypicans and other GPI-anchored proteins from the cell surface. (1/10093)

Glypicans are heparan sulfate proteoglycans that are attached to the cell surface by a GPI (glycosylphosphatidylinositol)anchor. Glypicans regulate the activity of Wnts, Hedgehogs,bone morphogenetic proteins and fibroblast growth factors. In the particular case of Wnts, it has been proposed that GPI-anchored glypicans stimulate Wnt signalling by facilitating and/or stabilizing the interaction between Wnts and their cell surface receptors. On the other hand, when glypicans are secreted to the extracellular environment, they can act as competitive inhibitors of Wnt. Genetic screens in Drosophila have recently identified a novel inhibitor of Wnt signalling named Notum. The Wnt inhibiting activity of Notum was associated with its ability to release Dlp [Dally (Division abnormally delayed)-like protein; a Drosophila glypican] from the cell surface by cleaving the GPI anchor. Because these studies showed that the other Drosophila glypican Dally was not released from the cell surface by Notum,it remains unclear whether this enzyme is able to cleave glypicans from mammalian cells. Furthermore, it is also not known whether Notum cleaves GPI-anchored proteins that are not members of the glypican family. Here, we show that mammalian Notum can cleave several mammalian glypicans. Moreover, we demonstrate that Notum is able to release GPI-anchored proteins other than glypicans. Another important finding of the present study is that,unlike GPI-phospholipase D, the other mammalian enzyme that cleaves GPI-anchored proteins, Notum is active in the extracellular environment. Finally, by using a cellular system in which GPC3 (glypican-3) stimulates Wnt signalling, we show that Notum can act as a negative regulator of this growth factor. (+info)

alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clustering. (2/10093)

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Influence of brain-derived neurotrophic factor on pathfinding of dentate granule cell axons, the hippocampal mossy fibers. (3/10093)

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Effective gene-viral therapy of leukemia by a new fiber chimeric oncolytic adenovirus expressing TRAIL: in vitro and in vivo evaluation. (4/10093)

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A novel human monoclonal antibody that binds with high affinity to mesothelin-expressing cells and kills them by antibody-dependent cell-mediated cytotoxicity. (5/10093)

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Regulation of dopamine transporter activity by carboxypeptidase E. (6/10093)

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Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models. (7/10093)

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Selenoprotein P regulation by the glucocorticoid receptor. (8/10093)

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As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. HEK 293 cells have a complex ... HEK 293 Transfection and Selection Data @ Cell-culture Database A HEK293 Cell Database 293 Cells (CRL-1573) Archived 2012-06-22 ... Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a ... They are quite similar to each other but show detectable divergence from the parental HEK 293 cell line. HEK 293 cells are ...

https://en.wikipedia.org/wiki/HEK_293_cells

With Graham's contributions in transfection of the cells with the adenovirus 5 this led to the cell line of HEK 293 cells. HEK ... "HEK293 Cell Line". hek293.com. Archived from the original on 16 October 2014. "In memoriam professor Mathieu Noteborn" (in ... he created the PER.C6 cell line with enhanced traceability compared to the HEK 293 line, this time coming from retinal cells. ... In contrast to HEK 293 the PER.C6 cell line was patented by Leiden University and IntroGene (later Crucell) and used ...

https://en.wikipedia.org/wiki/Lex_van_der_Eb

HEK 293 cells - derived from human fetal cells. Jurkat cells - a human T lymphocyte cell line isolated from a case of leukemia ... A549 cells - derived from a cancer patient lung tumor. HeLa cells - a widely used human cell line isolated from cervical cancer ... Immortalised cell lines have also found uses in biotechnology. An immortalised cell line should not be confused with stem cells ... OK cells - derived from female North American opossum kidney cells Ptk2 cells - derived from male long-nosed potoroo epithelial ...

https://en.wikipedia.org/wiki/Immortalised_cell_line

This was verified using GPR18 transfected HEK-293 cells. The same migration wasn't witnessed using non-transfected and GPR55 ... NAGly has been hypothesized to induce cell migration in BV-2 microglia cells. The same research suggests that this migration ... NAGly powerfully stimulates oxygen consumption in multiple cell lines, including murine C2C12 myoblasts and human HEK293T cells ... Cell. 166 (2): 424-435. doi:10.1016/j.cell.2016.05.071. PMC 4947008. PMID 27374330. Lin H, Long JZ, Roche AM, Svensson KJ, Dou ...

https://en.wikipedia.org/wiki/N-Arachidonylglycine

2007). "Upregulation of PHLDA2 in Dicer knockdown HEK293 cells". Biochim. Biophys. Acta. 1770 (5): 820-5. doi:10.1016/j.bbagen. ...

https://en.wikipedia.org/wiki/PHLDA2

"Subcellular localization of human neutral ceramidase expressed in HEK293 cells". Biochemical and Biophysical Research ...

https://en.wikipedia.org/wiki/ASAH2C

2005). "Subcellular localization of human neutral ceramidase expressed in HEK293 cells". Biochem. Biophys. Res. Commun. 331 (1 ... 2006). "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P". ...

https://en.wikipedia.org/wiki/ASAH2

However, the HEK 293 cells used for vaccine manufacturing, express several adenoviral genes, including the ones required for ... To manufacture the vaccine the virus is propagated on HEK 293 cell lines and then purified multiple times to completely remove ... Thomas P, Smart TG (2005). "HEK293 cell line: a vehicle for the expression of recombinant proteins". J Pharmacol Toxicol ... Cell Research. 18 (2): 290-301. doi:10.1038/cr.2008.15. PMC 7091891. PMID 18227861. "There are no foetal cells in the ...

https://en.wikipedia.org/wiki/Oxford–AstraZeneca_COVID-19_vaccine

Both Ad26 and Ad5 were modified to remove the E1 gene to prevent replication outside the HEK 293 cells. For the production of ... Rarely, Ad5 can acquire the E1 gene from the HEK 293 cells, restoring its ability to replicate. Gamaleya has set an acceptable ... Tan, Evan; Chin, Cara Sze Hui; Lim, Zhi Feng Sherman; Ng, Say Kong (2021). "HEK293 Cell Line as a Platform to Produce ... Large quantities of both adenoviruses are produced by HEK 293 cells that have the E1 gene necessary for viral replication. ...

https://en.wikipedia.org/wiki/Sputnik_V_COVID-19_vaccine

"Functional comparison of HCN isoforms expressed in ventricular and HEK 293 cells". Pflügers Archiv. 444 (5): 597-601. doi: ...

https://en.wikipedia.org/wiki/HCN4

The virus is grown in HEK 293 cells and purified for administration. It was developed by Spark Therapeutics and Children's ... due to inherited retinal dystrophy caused by confirmed biallelic RPE65 mutations and who have sufficient viable retinal cells. ...

https://en.wikipedia.org/wiki/Voretigene_neparvovec

Knock down of SIRT7 in HEK293 cells resulted in decreased rRNA levels. This same study found that this SIRT3 knockdown ... In humans cells, SIRT7 has only been shown to interact with two other molecules: RNA polymerase I (RNA Pol I) and upstream ... Mohrin M, Shin J, Liu Y, Brown K, Luo H, Xi Y, Haynes CM, Chen D (Mar 2015). "Stem cell aging. A mitochondrial UPR-mediated ... Sun L, Dang W (July 2020). "SIRT7 slows down stem cell aging by preserving heterochromatin: a perspective on the new discovery ...

https://en.wikipedia.org/wiki/Sirtuin_7

"Functional comparison of HCN isoforms expressed in ventricular and HEK 293 cells". Pflügers Archiv. 444 (5): 597-601. doi: ... Calloe K, Elmedyb P, Olesen SP, Jorgensen NK, Grunnet M (Sep 2005). "Hypoosmotic cell swelling as a novel mechanism for ... Cell. 93 (5): 717-29. doi:10.1016/S0092-8674(00)81434-8. PMID 9630217. S2CID 10265917. Hofmann F, Biel M, Kaupp UB (Dec 2005 ... "Identification of the cyclic-nucleotide-binding domain as a conserved determinant of ion-channel cell-surface localization". ...

https://en.wikipedia.org/wiki/HCN2

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... "Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells". Mol. Med. 8 (2): 95- ...

https://en.wikipedia.org/wiki/PRDX4

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... Cell. 122 (6): 957-68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. Guo D ... "A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death". ... 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell ...

https://en.wikipedia.org/wiki/CCT7

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ...

https://en.wikipedia.org/wiki/SUMO4

2006). "Acute hypoxic regulation of recombinant THIK-1 stably expressed in HEK293 cells". Adv. Exp. Med. Biol. ADVANCES IN ... 2008). "Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1) K channels in the mammalian kidney". Cell. Physiol. ...

https://en.wikipedia.org/wiki/KCNK13

December 2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and ...

https://en.wikipedia.org/wiki/GMP_synthase

December 2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and ... in in vitro differentiation and cell cycle behavior of HT-29-M6 intestinal mucosecretory cells". Molecular and Cellular Biology ... "Up-regulation of CIR1/CROC1 expression upon cell immortalization and in tumor-derived human cell lines". Oncogene. 17 (10): ... Cell. 96 (5): 645-53. doi:10.1016/S0092-8674(00)80575-9. PMID 10089880. S2CID 17117789. Deng L, Wang C, Spencer E, Yang L, ...

https://en.wikipedia.org/wiki/Kua-UEV

https://en.wikipedia.org/wiki/PAFAH1B3

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... Cell. Biol. 20 (9): 3157-67. doi:10.1128/MCB.20.9.3157-3167.2000. PMC 85610. PMID 10757800. Zhang QH, Ye M, Wu XY, Ren SX, Zhao ... Cell. Biol. 20 (9): 3157-67. doi:10.1128/mcb.20.9.3157-3167.2000. PMC 85610. PMID 10757800. Datta PK, Chytil A, Gorska AE, ... "High-throughput mapping of a dynamic signaling network in mammalian cells". Science. 307 (5715): 1621-5. doi:10.1126/science. ...

https://en.wikipedia.org/wiki/STRAP

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... Molecular Cell. 5 (5): 779-87. doi:10.1016/S1097-2765(00)80318-4. PMID 10882114. Will CL, Schneider C, MacMillan AM, Katopodis ... "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nature Biotechnology. 23 (1): 94-101. doi:10.1038/ ...

https://en.wikipedia.org/wiki/SF3B4

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... "Specific association of estrogen receptor beta with the cell cycle spindle assembly checkpoint protein, MAD2". Proc. Natl. Acad ... "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94-101. doi:10.1038/nbt1046. ...

https://en.wikipedia.org/wiki/EIF3I

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. Commun. ... "Identification of candidate radioresistant genes in human squamous cell carcinoma cells through gene expression analysis using ... Cell Biol. 6 (2): 97-105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986. Higo M, Uzawa K, Kouzu Y, Bukawa H, Nimura Y, ... Cell. Proteomics. 4 (11): 1725-40. doi:10.1074/mcp.M500231-MCP200. PMID 16085932. Human CCT6A genome location and CCT6A gene ...

https://en.wikipedia.org/wiki/CCT6A

"Synthetic ciguatoxins selectively activate Nav1.8-derived chimeric sodium channels expressed in HEK293 cells". The Journal of ... Voltage clamp has been used to show how action potentials in DRG cells are shaped by TTX-resistant sodium channels. Nav1.8 ... Lipid rafts organise the cell membrane, which includes trafficking and localising ion channels. Removal of lipid rafts in the ... Although NaV1.8 is not normally expressed within the cerebellum, its expression is up-regulated in cerebellar Purkinje cells in ...

https://en.wikipedia.org/wiki/Nav1.8

December 2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and ... Within cells, IDH1 has been observed to localize to the cytoplasm, peroxisome, and endoplasmic reticulum. Under hypoxic ... In addition, IDH1 is key to β-oxidation of unsaturated fatty acids in the peroxisomes of liver cells. IDH1 also participates in ... Vaccination of MHC-humanized transgenic mice with mutant IDH1 peptide induced an IFN-γ CD4+ T-helper 1 cell response, ...

https://en.wikipedia.org/wiki/IDH1

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... "The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells". Molecular Biology of the Cell. 9 ... "Interaction of ZPR1 with translation elongation factor-1alpha in proliferating cells". The Journal of Cell Biology. 143 (6): ... Gangwani L (December 2006). "Deficiency of the zinc finger protein ZPR1 causes defects in transcription and cell cycle ...

https://en.wikipedia.org/wiki/ZNF259

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... IDH3 activity is regulated by the energy needs of the cell: when the cell requires energy, IDH3 is activated by ADP; and when ... Within cells, IDH3 and its subunits have been observed to localize to the mitochondria. IDH3α expression has been linked to ... HIF-1 is largely known for shifting glucose metabolism from oxidative phosphorylation to aerobic glycolysis in cancer cells ( ...

https://en.wikipedia.org/wiki/IDH3A

"Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochemical and Biophysical ... "A product of the human gene adjacent to parkin is a component of Lewy bodies and suppresses Pael receptor-induced cell death". ... 1 changes the content of a particular subunit species concomitant with substrate binding and folding activities during the cell ...

https://en.wikipedia.org/wiki/CCT2_(gene)

2005). "Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress". Biochem. Biophys. Res. ... 2008). "Direct role of nucleotide metabolism in C-MYC-dependent proliferation of melanoma cells". Cell Cycle. 7 (15): 2392-400 ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ... doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685. He Y, Mou Z, Li W, et al. (2009). "Identification of IMPDH2 as a ...

https://en.wikipedia.org/wiki/IMPDH2

Using a β2 adrenergic receptor preparation derived from transfected HEK 293 cells, Liappakis and co-workers found that in wild- ... Measurements of cAMP accumulation in intact transfected HEK 293 cells, after treatment with EEDQ to inactivate 98-99% of the ...

https://en.wikipedia.org/wiki/Halostachine

"Sodium-dependent norepinephrine-induced currents in norepinephrine-transporter-transfected HEK-293 cells blocked by cocaine and ... As much as 90% of the norepinephrine released will be taken back up in the cell by NET. NET functions by coupling the influx of ... The gradient is generated by the Na+/K+-ATPase which transports three sodium ions out and two potassium ions into the cell. ... The transporters can be found along the cell body, axons, and dendrites of the neuron. NETs are located away from the synapse, ...

https://en.wikipedia.org/wiki/Norepinephrine_transporter

HEK 293t cells transfected with either EGFP-ADAR1, EGFP-ADAR2 or untransfected HEK293 cells were used to determine which ADAR ... The editing level at the Y/C site was 16% while in tumour cells was an average of 21% in brain. It has been shown that ADAR1 ... However it is a widely believed that the protein is involved in tumour suppression by decreasing cell growth through initiating ... Inosines are recognised as guanosine by the cells translational machinery. There are three members of the ADAR family ADARs 1-3 ...

https://en.wikipedia.org/wiki/BLCAP

"Riboswitch-mediated Attenuation of Transgene Cytotoxicity Increases Adeno-associated Virus Vector Yields in HEK-293 Cells". ... Pyrithiamine pyrophosphate has been shown to bind and activate the TPP riboswitch, causing the cell to cease the synthesis and ... Because pyrithiamine pyrophosphate does not substitute for TPP as a coenzyme, the cell dies. Since riboswitches are an ... For example, when the antibiotic pyrithiamine enters the cell, it is metabolized into pyrithiamine pyrophosphate. ...

https://en.wikipedia.org/wiki/Riboswitch

treated HEK 293 cells with agents causing oxidative DNA damage, (potassium bromate (KBrO3) or potassium chromate (K2CrO4)). ... After DNA damage, cell cycle checkpoints are activated. Checkpoint activation pauses the cell cycle and gives the cell time to ... Thus, in a population of cells composing a tissue with replicating cells, mutant cells will tend to be lost. However, ... Mutations are replicated when the cell replicates. In a population of cells, mutant cells will increase or decrease in ...

https://en.wikipedia.org/wiki/DNA_repair

number of sources - referenced in - Subcellular localisation of recombinant Densin-180 clones expressed in HEK293 TSA cells ... Subcellular localisation of recombinant Densin-180 clones expressed in HEK293 TSA cells Ranatunga, J.M. (2011) Subcellular ... Lassila M, Juhila J, Heikkilä E, Holthöfer H (2007). "Densin is a novel cell membrane protein of Sertoli cells in the testis". ... 2007). "Densin and beta-catenin form a complex and co-localize in cultured podocyte cell junctions". Mol. Cell. Biochem. 305 (1 ...

https://en.wikipedia.org/wiki/LRRC7

... in HEK-293 cells". Mol. Cell. Biochem. 224 (1-2): 123-33. doi:10.1023/A:1011907328682. PMID 11693189. S2CID 26037173. Hagner S ... 2001). "Differential and cell-specific expression of calcitonin receptor-like receptor and receptor activity modifying proteins ... 1999). "The RAMP2/CRLR complex is a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells". ... 2000). "Dexamethasone increases RAMP1 and CRLR mRNA expressions in human vascular smooth muscle cells". Biochem. Biophys. Res. ...

https://en.wikipedia.org/wiki/CALCRL

2000). "LDL binds to surface-expressed human T-cadherin in transfected HEK293 cells and influences homophilic adhesive ... 2001). "Expression of cell adhesion molecule T-cadherin in the human vasculature". Histochem. Cell Biol. 115 (3): 231-42. doi: ... In confluent cultures of vascular cells, T-cadherin was distributed equally over the entire cell surface, in contrast to VE- ... 2003). "Cadherin-13, a mediator of calcium-dependent cell-cell adhesion, is silenced by methylation in chronic myeloid leukemia ...

https://en.wikipedia.org/wiki/T-cadherin

The experiment involved transfecting HEK 293 cells with a Bax-encoding plasmid, which resulted in an increase in apoptosis (~7 ... human lung cancer cell line with wild-type p53) and T47D (human breast cancer cell line with mutant p53) cells with DNA- ... This cell cycle arrest causes a dramatic decline in survivin levels in the cells, as it is known from the literature that ... Yeast cells with knock-outs of certain IAP genes did not show problems associated with cell death, but showed defects in ...

https://en.wikipedia.org/wiki/Survivin

Thereafter 1-6 µg of plasmidial DNA is transfected by electroporation into QMCF cells (CHO or HEK293 based cells) for ... QMCF cell lines express Large-T antigen and EBNA-1 proteins which bind the viral sequences on the QMCF plasmid and hence ... Compared to usage of stable cell line, QMCF technology is a rapid method leaving out time-consuming clone selection step during ... Since QMCF plasmids contain antibiotic resistance gene and are able to stably replicate and remain inside dividing cells, a ...

https://en.wikipedia.org/wiki/QMCF_Technology

... receptors in HEK293 cells and B-lymphocytes". FEBS Letters. 538 (1-3): 159-62. doi:10.1016/S0014-5793(03)00172-8. PMID 12633871 ... On T cells activation of P2X7 receptors can activate the T cells or cause T cell differentiation, can affect T cell migration ... Boison D, Chen JF, Fredholm BB (July 2010). "Adenosine signaling and function in glial cells". Cell Death and Differentiation. ... In melanocytic cells P2X7 gene expression may be regulated by MITF. Activation of the P2X7 receptor by ATP leads to recruitment ...

https://en.wikipedia.org/wiki/P2RX7

3-FPM also inhibits uptake mediated by dopamine transporters and norepinephrine transporters in HEK293 cells with potencies ...

https://en.wikipedia.org/wiki/3-Fluorophenmetrazine

African green monkey kidney cells) and in HEK 293 (human embryonic kidney cells) instead of embryonated chicken eggs, also ... Not all cancer cells have cell entry receptors for the virus and not all cancer cells express virus processing serine proteases ... involves the fusion of infected cells with adjacent target cells and remains an important mechanism of direct cell-to-cell ... "Directed cell fusion" below). The virus can fuse up to 50-100 cells adjacent to one primary infected cell. This multi-nuclear ...

https://en.wikipedia.org/wiki/Murine_respirovirus

... for example differences in glycosylation between rodent and human cells. Even within one cell line, often stabilizing a cell ... and the HEK293 human kidney line. A common protist eukaryotic expression system is the slime mold, Dictyostelium discoideum, ... In this method, the viral vector (virion) infects host cells that by directly transporting DNA into the nucleus of the cell. ... The only commercially viable way to use mammalian cells as host systems is a high value end product. Common mammalian cell ...

https://en.wikipedia.org/wiki/Heterologous_expression

"Chelerythrine chloride induces apoptosis in renal cancer HEK-293 and SW-839 cell lines". Oncology Letters. 11 (6): 3917-3924. ... This molecule has the ability to disrupt a bacteria's cell wall and cell membrane, as well as preventing bacterial growth, all ... This further contributes to the loss of calcium ion balance within the cell and eventual cell death. In triple-negative breast ... These mechanisms include inducing apoptosis, arrest of the cell cycle, promoting autophagy of cancerous cells, and the ...

https://en.wikipedia.org/wiki/Chelerythrine

In addition to cell trafficking, in vitro studies conducted on HEK293 cell revealed that MRAP enhances MC2 response to ACTH ... MRAP mutations were found to disable the movement of MC2 to the cell surface of adrenal gland cells; this would make MC2 ... and also on the cell membrane, which indicates that MC2 needs MRAP to reach the cell membrane. ... In fat cells, where MC2 is expressed, MRAP was found to facilitate MC2 activated lipolysis and therefore regulating energy ...

https://en.wikipedia.org/wiki/Melanocortin_2_receptor_accessory_protein

DP1 activation also causes the mobilization of calcium in HEK293 cells transfected with this receptor. It does so by a ... Th2 cells, and dendritic cells, and by cells contributing to these reactions, i.e. human and/or rodent airway epithelial cells ... see T helper cell#Th1/Th2 Model for helper T cells and T helper cell#Limitations to the Th1/Th2 model. DH1 activation also ... vascular endothelium, mucus-secreting goblet cells in the nasal and colonic mucosa, and serous gland cells of the nose. DP1 ...

https://en.wikipedia.org/wiki/Prostaglandin_DP1_receptor

"Functional and radioligand binding characterization of rat 5-HT6 receptors stably expressed in HEK293 cells". Neuropharmacology ...

https://en.wikipedia.org/wiki/5-Methoxytryptamine

... later studies indicate that Naa11 is not expressed in the human cell lines HeLa and HEK293 or in cancerous tissues, and NAA11 ... including cell migration, cell cycle control, DNA damage control, caspase-dependent cell death, p53 dependent apoptosis, cell ... Cell. 146 (4): 607-20. doi:10.1016/j.cell.2011.06.050. PMC 3182480. PMID 21854985. Kuo HP, Lee DF, Chen CT, Liu M, Chou CK, Lee ... Two alternative splicing products of mouse Naa10, mNaa10235 and mNaa10225, were reported in NIH-3T3 and JB6 cells that may have ...

https://en.wikipedia.org/wiki/N-alpha-acetyltransferase_10

When expressed in naïve HEK293 cells, synphilin 1 forms multiple small highly mobile aggregates. However, proteasome or Hsp90 ... In eukaryotic cells, an aggresome refers to an aggregation of misfolded proteins in the cell, formed when the protein ... Consequently, elaborate systems have evolved to protect cells from the deleterious effects of misfolded proteins. Cells mainly ... An aggresome forms around the microtubule organizing center in eukaryotic cells, adjacent to or enveloping the cell's ...

https://en.wikipedia.org/wiki/Aggresome

The inositol phosphate (IP3) accumulation, aequorin, and 35S isotope binding assays in overexpressing HEK293 cells have ... GPR97 is transcribed in immune cells. Gene-deficient mice revealed that Gpr97 is crucial for maintaining B-cell population via ... Human lymphatic endothelial cells (LECs) abundantly express GPR97. Silencing GPR97 in human LECs indicated that GPR97 modulates ... GPR97 was found to be differently methylated at CpG sites in the genome of blood cells from patient under systemic steroid ...

https://en.wikipedia.org/wiki/GPR97

... receptor currents in cultured HEK-293 and N1E-115 cell systems in a non-competitive manner". Neuroscience Letters. 306 (1-2): ...

https://en.wikipedia.org/wiki/Memantine

The cell line can be cultured in an in-house media and in commercially-available serum-free media but weak growth profiles are ... This study aimed to evaluate the effect of insulin on HEK293SF-3F6 cell growth in two types of media while increasing influenza ... HEK293SF-3F6 is a suspension-adapted cell line that has successfully been used for the production of the influenza virus, virus ...

https://www.pharmaceutical-business-review.com/casestudies/insulin-increases-influenza-virus-yield-in-hek293-cells-for-vaccine-production/

Programmed Cell Death 1 Ligand 1; find Sigma-Aldrich-SRP0632 MSDS, related peer-reviewed papers, technical documents, similar ... expressed in HEK 293 cells; Synonyms: B7 homolog 1,B7-H1,CD274,PD-L1,PDCD1L1, ... expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avitag™ technology. MW = 54 kDa. ... Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7-H1) with a ...

https://www.sigmaaldrich.com/MX/es/product/sigma/srp0632

For flow cytometry or cell fusion. Functionally validated. Mycoplasma free. ... HEK293 cells engineered to overexpress the original Wuhan‑Hu-1 SARS‑CoV-2 Spike (S) protein. ... 293-SARS2-S Cells: with a functional furin cleavage site and recommended for cell-cell fusion assays with ACE2-expressing cells ... HEK293 ACE2-(TMPRSS2)-expressing Cell Line. • A549 ACE2-TMPRSS2-expressing Cell Line. • SARS-CoV-2 Spike Antibodies ...

https://www.invivogen.com/293-sars2-spike

Blocking clathrin-mediated endocytosis (CME) in HEK cells expressing the Kv4.2 TC mimicked and occluded the effect of SUMO on ... We previously demonstrated that when expressed in human embryonic kidney (HEK) cells, Kv4.2 can be SUMOylated at two K residues ... We previously demonstrated that when expressed in human embryonic kidney (HEK) cells, Kv4.2 can be SUMOylated at two K residues ... of the Kv4.2 Ternary Complex Increases Surface Expression and Current Amplitude by Reducing Internalization in HEK 293 Cells. ...

https://www.frontiersin.org/articles/10.3389/fnmol.2021.757278/full

Allocryptopine and benzyltetrahydropalmatine block hERG potassium channels expressed in HEK293 cells ... current expressed in HEK293 cells.. Methods: Cultured HEK293 cells were transiently transfected with hERG channel cDNA plasmid ... Allocryptopine and benzyltetrahydropalmatine block hERG potassium channels expressed in HEK293 cells Kun Lin, Yu-qi Liu, Bin Xu ... The whole-cell current IHERG was evoked and recorded using Axon MultiClamp 700B amplifier. The drugs were applied via ...

http://www.chinaphar.com/article/view/5917

... cells were investigated using inside-out excised membrane patches. The relative permeability of these rOCNC1 channels to ... Ion permeation and selectivity of wild-type recombinant rat CNG (rOCNC1) channels expressed in HEK293 cells. Abstract. The ... Ion permeation and selectivity of wild-type recombinant rat CNG (rOCNC1) channels expressed in HEK293 cells ... Ion permeation and selectivity of wild-type recombinant rat CNG (rOCNC1) channels expressed in HEK293 cells ...

https://www.garvan.org.au/research/publications/1396

https://www.mayconsulting.cz/tag/recombinant-phenotype/

Plasmids for overexpression of a protein of interest in human HEK 293 cells using the HIV-1 TAR-Tat system. ... Vectors for ultra-high protein expression in HEK 293 cells using TAR-Tat transactivation. The pHEK293 Ultra Expression Vectors ... Ultra-high expression of recombinant proteins in HEK 293 cells. Additional product information. Please see the products ... The pHEK293 Ultra Expression Vectors are plasmids for transient overexpression of a protein of interest in human HEK 293 cells ...

https://www.takarabio.com/products/protein-research/expression-vectors-and-systems/mammalian-expression/phek293-ultra-expression-vectors

Suspension HEK293F cells (Invitrogen) grown in serum-free 293 Freestyle medium (Invitrogen). ...

https://gptwiki.glyomics.org/gptwiki/SA000002

Overexpression of the Heterochromatinization Factor BAHD1 in HEK293 Cells Differentially Reshapes the DNA Methylome on ... Overexpression of the Heterochromatinization Factor BAHD1 in HEK293 Cells Differentially Reshapes the DNA Methylome on ...

https://www.france-genomique.org/publication/overexpression-of-the-heterochromatinization-factor-bahd1-in-hek293-cells-differentially-reshapes-the-dna-methylome-on-autosomes-and-x-chromosome/?lang=en

HEK293 cell line expresses the Renilla Luciferase, containing Puromycin selection. ... GFP & RFP HEK293 stable cells. $1,400.00. Add to cart. * HEK293-KCNN4 cell line with RFP and Blasticidin marker. $3,000.00. Add ... Home / Stable cell lines / Luciferase cells / Luciferase (Renilla), HEK293 stable cells, (Puromycin). View cart "Luciferase ( ... Luciferase (Renilla), HEK293 stable cells, (Puromycin). $650.00. Luciferase (Renilla), HEK293 stable cells, (Puromycin) ...

https://www.gentarget.com/product/luciferase-renilla-hek293-stable-cells-puromycin/?add-to-cart=9273

Bioauxilium specializes in the design, development and manufacturing of ready-to-use TR-FRET assay kits that simplify laboratory workflow and accelerate drug discovery and life sciences research ...

https://bioauxilium.com/bioauxilium-z-factor-determination-hek293-cells-2/

This HEK293-hNRP1-hACE2 stable cell line expresses a recombinant human Neuropilin-1 (NRP-1) and a human Angiotensin-Converting ... This HEK293-hNRP1-hACE2 stable cell line expresses a recombinant human Neuropilin-1 (NRP-1) and a human Angiotensin-Converting ... Home , Pseudovirus and Related Cell Lines , Human Neuropilin-1 (NRP-1) Stable Cell Line ... Human Neuropilin-1 (NRP-1) Stable Cell Line. Catalog Number: CL-NRP1-001 ...

https://www.eenzyme.com/CL-NRP1.aspx

We found that in HEK293 cells, the SCN5A-p.Y1977N mutation abolished the interaction between Nav1.5 and Nedd4-2, suppressed PY- ... both in HEK293 cells as well as in newly generated mice harboring the mouse homolog mutation Scn5a-p.Y1981N. ... 4.2.1. Cell Culture. The HEK293 cell line has been purchased from A.T.C.C (ATCC-CRL-1573, Manassas, VA, USA). HEK293 cells were ... Life Technologies, Basel, Switzerland). HEK293 cells were maintained at 37 °C in a 5%/95% CO2/O2 incubator. HEK293 cells were ...

https://www.mdpi.com/1422-0067/20/20/5033

Review clonality and improved outgrowth for HEK 293 cells when developing stable producer cell lines for viral vector ... Gene and cell therapy workflows frequently utilize HEK293/293T cells in the production of viral vectors. ... Improved Outgrowth For HEK 293 Cell Line Development. Source: Advanced Instruments, Inc. ... Read how the use of VIPS and InstiGRO HEK were found to be a strong combination for developing HEK based cell lines whilst ...

https://www.cellandgene.com/doc/improved-outgrowth-for-hek-cell-line-development-0001

Adaptor Protein Ruk1 Forms Protein-Protein Complexes with Endonuclease Activity in HEK293 Cells. / Kit, Yu Ya; Drel, V. R.; ... Adaptor Protein Ruk1 Forms Protein-Protein Complexes with Endonuclease Activity in HEK293 Cells. In: Biochemistry (Moscow). ... Such complexes were isolated from lysates of HEK293 cells by chromatography on heparin-Sepharose. By elution with 0.5 and 1.0 M ... Adaptor Protein Ruk1 Forms Protein-Protein Complexes with Endonuclease Activity in HEK293 Cells. Biochemistry (Moscow). 2003 ...

https://scholars.uthscsa.edu/en/publications/adaptor-protein-ruksub1sub-forms-protein-protein-complexes-with-e

The results demonstrate Dynamis Medium to be effective for suspension HEK293 cell growth and adenovirus production. ... Simple fed-batch HEK293 cell growth in Dynamis Medium. Glucose was fed to 6 g/L when levels dropped below 3.5 g/L. HEK293 cells ... Suspension HEK293 cells cultured in Dynamis Medium showed increasing VCD for more than 7 days. On day 8 post cell seeding at ... Cell culture conditions. Growth performance was evaluated in simple fed-batch conditions with suspension HEK293 cells in ...

https://cellculturedish.com/optimization-conditions-support-efficient-hek293-cell-growth-adenovirus-production/

CHO-K1/HEK293 Stable Cells Lines, P2RY13 (Protein accession # NM_023914), High Quality-Price Ratio Products and Services. ... GPCR Cell Lines > CHO-K1/HEK293 Cells Stable expressing GPCR protein P2RY13 (Protein accession # NM_023914) CHO-K1/HEK293 Cells ... CHO-K1/HEK293 Cells Stable expressing GPCR protein P2RY13 (Protein accession # NM_023914) ...

https://www.angioproteomie.com/commerce/ccp6504-cho-k1-hek293-cells-stable-expressing-gpcr-protei-cap-1336.htm

Human embryonic kidney cell line, cryovial ... Cell Culture * BioConcept Products * Customized Cell Culture ...

https://www.bioconcept.ch/de/Products/Cell-Culture/Cells/HEK293_-Human-embryonic-kidney-56161-56161.html

Whole-cell voltage-clamp recordings in human embryonic kidney (HEK) 293 cells demonstrated that R1279P significantly ...

https://pubmed.ncbi.nlm.nih.gov/25209274/

HEK293 Cells. C-His. 90294-C08H. Cynomolgus. TGF-β. HEK293 Cells. Active. C-His. ... HEK293 Cells. C-His. 80116-RNAH. Mouse, Rat. TGF-β. HEK293 Cells. Active. Native. ... CHO Stable Cells. Active. Native. 50698-M08H-B. Mouse. TGF-β. ... HEK293 Cells. C-His. 70003-DNAE. Canine. TNF-α. E. coli. Active ... HEK293 Cells. Active. C-His. 10804-HNAC. Human, Rhesus, Cynomolgus, Canine. TGF-β. ...

https://www.news-medical.net/Pro-and-anti-inflammatory-cytokines-in-chronic-inflammation

Cell Culture Immune System Inflammation Neurobiology Stem Cells & Differentiation TGF-β Superfamily Wound Healing Allergy ... Biological Activity: Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. The ED50 ... They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. The ... Synonyms: Transforming Growth Factor-β2, Glioblastoma-derived T cell suppressor factor, BSC-1, Cetermin, Polyergin ...

https://www.peprotech.com/en/recombinant-human-tgf-2-hek293-derived

A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization. / Ooi, Amanda Siok Lee; ... A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization. Frontiers in Physiology ... A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization. In: Frontiers in ... The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins ...

https://academia.kaust.edu.sa/en/publications/a-guide-to-transient-expression-of-membrane-proteins-in-hek-293-c

... expressed in HEK 293 cells; find Sigma-Aldrich-ZMS1017 MSDS, related peer-reviewed papers, technical documents, similar ... and NIH3T3 cells.. Flow Cytometry Analysis: 1 μg from a representative lot detected DNA-RNA hybrid in one million A431 cells.. ... Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected DNA-RNA hybrid in HeLa cells.. Tested applications. ... Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected DNA-RNA hybrid in HeLa cells. Note: Actual optimal ...

https://wwwqws.sigmaaldrich.com/US/en/product/sigma/zms1017

Stably transfected HEK 293 cells expressing the a variety of hMOR-one variants have been attained right after around two months ... Live HEK 293 human embryonic kidney cells (American Kind Tradition Collection) had been seeded on 35 mm glass base cirradiated ... uncategorized , Stably transfected HEK 293 cells expressing the a variety of hMOR-one variants have been attained right after ... 20 4 several hours soon after seeding, HEK 293 cells were transfected making use of FuGene High definition (Roche) and GFP- ...

https://dhfrinhibitor.com/2016/05/24/stably-transfected-hek-293-cells-expressing-the-a-variety-of-hmor-one-variants-have-been-attained-right-after-around-two-months-of-selection-with-g418-five-hundred-mgml/

... coimmunoprecipitation experiments were performed in HEK293T cells transfected with GAPDH and Mst1 expression vectors. ... To determine whether or not the interaction among Mst1 and GAPDH happens in mammalian cells, ... B, Mst1 immunoprecipitated from HEK293 cells transfected with possibly Myc-Mst1 or Myc-Mst1 as well as Flag-GAPDH expression ... To more map the conversation domains of GAPDH and Mst1, we carried out immunoprecipitation in HEK293 cells cotransfected with ...

http://www.adenosine-receptor.com/2016/01/04/to-more-map-the-conversation-domains-of-gapdh-and-mst1-we-carried-out-immunoprecipitation-in-hek293-cells-cotransfected-with-diverse-deletion-mutants-of-mst1-and-gapdh/

Protective effects of emodin against cisplatin-induced oxidative stress in cultured human kidney (HEK 293) cells. ... HEK 293) cells. Together they form a unique fingerprint. ...

https://research.uaeu.ac.ae/en/publications/protective-effects-of-emodin-against-cisplatin-induced-oxidative-/fingerprints/

HEK293) from Native Antigen. Cat Number: REC32008. UK & Europe Distribution. Order Online. ... HEK293 N SARS-CoV-2 Nucleocapsid Overexpression Stable Cell Line , CVD19-220A-1 SBI System Biosciences ... SARS-CoV-2 (B.1.1.529/Omicron) Stabilized Spike Glycoprotein (Trimeric), His-Strep-Tag (HEK293). (No reviews yet) Write a ... PRODUCT DETAILS - SARS-COV-2 (B.1.1.529/OMICRON) STABILIZED SPIKE GLYCOPROTEIN (TRIMERIC), HIS-STREP-TAG (HEK293). *SARS-CoV-2 ...

https://proteomecommons.org/sars-cov-2-b-1-1-529-omicron-stabilized-spike-glycoprotein-trimeric-his-strep-tag-hek293/

Recombinant Protein Western Blot/HEK293 Cells Overexpression Assay. Amphiphysin, MAP1B, and CRMP5 antibody specificities were ... median CSF cell count 8 cells/mm3, range 0-74 cells/mm3 [lymphocytic predominant] and median CSF protein 73 mg/dL, range 31-188 ... 25 KLHL11 IgG detected on tissue immunofluorescence was confirmed by KLHL11 HEK293 overexpression cell-based assay.22,26 ... Purkinje cell cytoplasmic antibody type 1 [PCA1] [anti-Yo], n = 1; Purkinje cell cytoplasmic antibody type 2 [PCA2], n = 2; ...

https://n.neurology.org/content/96/4/e632

Compound screening in cell-based models of tau inclusion formation: Comparison of primary neuron and HEK293 cell assays. ... we performed a gene microarray-based transcriptome analysis of neuronal cells derived from human induced pluripotent stem cells ... Using a cell-based inclusion seeding assay, we recently reported that only a few tau variants are intrinsically prone to this ... Clearance of intracellular tau protein from neuronal cells via VAMP8-induced secretion. Journal of Biological Chemistry ...

https://www.jbc.org/Molecular-Bases-Disease?startPage=&KeywordRaw=Alzheimer%20disease

We previously demonstrated that when expressed in human embryonic kidney (HEK) cells, Kv4.2 can be SUMOylated at two K residues, K437 and K579. (frontiersin.org)
The permeation properties of adenosine 3', 5'-cyclic monophosphate (cAMP)-activated recombinant rat olfactory cyclic nucleotide-gated channels (rOCNC1) in human embryonic kidney (HEK 293) cells were investigated using inside-out excised membrane patches. (garvan.org.au)
Whole-cell voltage-clamp recordings in human embryonic kidney (HEK) 293 cells demonstrated that R1279P significantly depolarizes steady-state fast-, slow-, and closed-state inactivation. (nih.gov)
The human embryonic kidney 293 (HEK-293) cells are commonly used as host for the heterologous expression of membrane proteins not least because they have a high transfection efficiency and faithfully translate and process proteins. (edu.sa)
Live HEK 293 human embryonic kidney cells (American Kind Tradition Collection) had been seeded on 35 mm glass base cirradiated tissue culture dishes (MatTek Corp.), and transfected with hMOR/pcDNA3 constructs following 24 h utilizing the FuGENE 6 transfection reagent (Roche). (dhfrinhibitor.com)
To extend our knowledge of the pharmacological profile of human α4β2 neuronal nicotinic receptors, we investigated the action of hexamethonium on the major brain human nicotinic acetylcholine receptor (nAChR) stably expressed in human embryonic kidney 293 cells. (semanticscholar.org)
We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatography of recombinant antibodies expressed transiently in mammalian human embryonic kidney 293T-cells at the 1-L scale. (cyberleninka.org)
A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5. (bvsalud.org)
Human embryonic kidney (HEK293) cells expressing the human isoforms of SERT and DAT were used for the uptake inhibition and release assays. (iqs.edu)
DC - The news circulating wildly that President Trump's medical treatment for COVID-19 included therapies developed using embryonic stem cells and thereby destroying human life in the process are false, two medical scholars are saying on record. (illinoisreview.com)
Instead, the experimentation was done on mouse embryonic cell lines and blood from recovered SARS-COV-2 patients. (illinoisreview.com)
Uninformed commentary has emerged this morning stating that President Trump has received a medication created with the use of human embryonic stem cells. (illinoisreview.com)
No human embryonic stem cells or human fetal tissue were used to produce the treatments President Trump received-period. (illinoisreview.com)
The Regeneron therapy given to the president was made in Velocimmune humanized mice, a novel platform that uses genetically modified mouse embryonic stem (ES) cells to generate antibodies described here and here . (illinoisreview.com)
Mouse embryonic stem cells and genetic modifications to make such mice date back to 1981, have been extensively studied, and were instrumental in the discovery of induced pluripotent stem (iPS) cells, another fully ethical alternative to fetal material, as discussed in this Nature review. (illinoisreview.com)
iPSCs are much easier to use than human embryonic stem cells, more flexible in their uses, and are not ethically controversial. (illinoisreview.com)
No one has ever advocated against using mouse embryonic stem cells for development of therapies - only against destruction of human lives. (illinoisreview.com)

Human secreted Programmed Cell Death 1 Ligand 1 (PD-L1)-Fc fusion protein, also known as CD274 and B7 homolog 1 (B7-H1) with a T20Q mutation,GenBank Accession No. NM_014143,amino acids 19-239, fused at the Cterminus with the Fc portion of human IgG1, with C-terminal Avitag ™ expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avitag ™ technology. (sigmaaldrich.com)
The pHEK293 Ultra Expression Vectors are plasmids for transient overexpression of a protein of interest in human HEK 293 cells. (takarabio.com)
To study the nature and biological role of such complexes, the recombinant protein Ruk 1 with a Glu-epitope at the C-terminus (Ruk 1 Glu-tagged) was purified from transfected HEK293 cells by affinity chromatography on protein G-Sepharose with covalently conjugated anti-Glu-tag antibodies. (uthscsa.edu)
These findings suggest the presence of DNases associated with the Ruk 1 protein in HEK293 cells. (uthscsa.edu)
It was concluded that the adaptor protein Ruk 1 forms complexes with endonucleases in HEK293 cells. (uthscsa.edu)
For example, Rosetta™ cells are used for protein constructs containing a high number of rare E. coli codons, and Origami™ cells are used to encourage proper disulfide bond formation. (bcm.edu)
We provide an efficient Ligation-Independent Cloning (LIC) system (Figure 1), which allows simultaneous cloning of genes into a large set of customized expression vectors (Figure 2) for protein expression in E. coli, insect cells and mammalian cells. (nki.nl)
Later, they transduced the CRISPR-competent iPSCs en masse with a library of sgRNAs targeting each protein-coding gene in the genome. (alzforum.org)
ORMDL3 V1 open reading frame was subcloned into pEGFP-C1 vector and it was found that the protein synthesis had been followed in transfected Hela cells. (curehunter.com)
Providing our customers with innovative bioanalytical solutions for protein and cell biology for over 30 years. (moleculardevices.com)
Recombinant human Anthrax toxin receptor 2 / ANTXR2 (transcript variant 2) protein expressed in HEK293 cells. (antikoerper-online.de)
Description: Host cell factor 2(HCFC2) is a cytoplasmic protein. (gentaurtop.com)
Whilst the purification of the full-length huntingtin protein from the insect cell system we have working in the lab, gives high yields so plenty of protein to do experiments with, the protein is not made in a way which 100 % represents how it is made in humans. (labscribbles.com)
SARS-CoV-2, Recombinant Protein, Spike RBD (YP_009724390.1) (A348T) mutant, expressed from HEK293 cells, with a polyhistidine tag at the C-terminus. (creative-biolabs.com)
These results were disheartening so I began exploring alternative means of expressing HTT in cells, including protein transfection and protein electroporation. (openlabnotebooks.org)
Using the methods from PMC6656429 , and conditions from the manufacturer , I began with electroporating some HEK293 cells with GFP protein to identify conditions where I got the highest level of protein electroporation and sufficiently high viability post-electroporation. (openlabnotebooks.org)
I noted that the protein electroporation is most successful with higher levels of protein concentration and with post-electroporation treatment of cells with trypsin. (openlabnotebooks.org)
Antibodies can also be introduced into cells in this manner, as well as sgRNAs/cas9 protein for cell engineering. (openlabnotebooks.org)
GFP protein electroporation in HEK293 cells. (openlabnotebooks.org)
BacMAM HTT protein expression in SH-SY5Y cells. (openlabnotebooks.org)
Predicting carrier-mediated hepatic disposition of rosuvastatin in man by scaling from individual transfected cell-lines in vitro using absolute transporter protein quantification and PBPK modeling. (tno.nl)
The most direct way to correct for differences in transporter abundance between cell-lines and tissue is by using absolute protein quantification. (tno.nl)
The abundance of these transporter proteins in the outer membranes of HEK293-cells, in human primary hepatocytes and in human liver tissue was determined by LC-MS/MS. The measured activity, corrected for protein abundance and scaled to the whole liver, gave a very accurate prediction of the hepatic intrinsic clearance observed in vivo. (tno.nl)
Semack, A, Malik, RU & Sivaramakrishnan, S 2016, ' G protein-selective GPCR conformations measured using FRET sensors in a live cell suspension fluorometer assay ', Journal of Visualized Experiments , vol. 2016, no. 115, e54696. (umn.edu)
Evaluation of protein-protein interactions in residing cells by protein micropatterning is at present restricted to the spatial association of transmembrane proteins and their corresponding downstream molecules. (aabioetica.org)

The pHEK293 Ultra Expression Vectors are plasmid vectors developed for transient overexpression of recombinant proteins in human HEK293 cells. (takarabio.com)
Nevertheless, the heterologous expression of complex membrane proteins such as receptors and ion channels for biological characterization and in particular for single-cell applications such as electrophysiology remains a challenge. (edu.sa)
Here, we use the heterologous expression of a plant potassium channel, the Arabidopsis thaliana guard cell outward-rectifying K+ channel, AtGORK (At5G37500) in HEK-293 cells as an example, to evaluate commonly used transfection reagents and fluorescent detection methods, and provide a detailed methodology for optimized transient transfection and expression of membrane proteins for in vivo studies in general and for single-cell applications in particular. (edu.sa)
The infected Sf9 cells (for intracellular proteins) or culture supernatant (for secreted proteins) are provided to the investigator for expression screening and identification of the isolate with the highest level of expression. (bcm.edu)
We express proteins of interest(POIs) in insect cells using recombinant baculovirus. (bcm.edu)
Insect cell (Sf9): Baculovirus constructs are prepared to infect Sf9 insect cells for expression of both intracellular and secreted proteins. (nki.nl)
Using RNA sequencing, ribosome profiling, and proteomics data from human tissues and cell lines, we provide evidence for the translational activities of Alu exons and the presence of Alu exon derived peptides in human proteins. (biomedcentral.com)
Electroporation has also been reported to successfully deliver proteins into cells. (openlabnotebooks.org)
While the method did not work for this specific application, it does offer a useful approach to introduce difficult to express proteins into cells and examine how they behave within cells. (openlabnotebooks.org)

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected DNA-RNA hybrid in HeLa cells. (sigmaaldrich.com)
Principal antibody against calnexin (AbCam, ab 13504, an endoplasmic reticulum marker) was diluted 1:a hundred in blocking resolution (as earlier mentioned) and additional to the cells for 45 min at RT. (dhfrinhibitor.com)
Cells had been washed 3 moments in PBS and incubated with secondary antibody (Alexa Fluor 647 goat anti-rabbit, A21244, Invitrogen, one:a thousand in blocking remedy) for thirty min at RT, then washed three instances in PBS and saved in PBS at 4uC right up until examined by confocal laser scanning microscopy (usually the following working day). (dhfrinhibitor.com)
Lysates from transfected HEK293T cells have been immunoprecipitated with anti-myc antibody and analyzed by Western blot examination making use of anti-Flag and anti-Myc antibodies. (adenosine-receptor.com)
Lysates from transfected HEK293T cells were immunoprecipitated with anti-Myc antibody and analyzed by Western blotting examination using anti-Flag and anti?Myc-antibodies. (adenosine-receptor.com)
Cells are cultured in roller bottles and - depending on expression levels - several milligrams of antibody can be produced and purified. (nki.nl)
We observed a similar frequency (80%) of T-cell immunity in convalescent donors with strong antibody responses but did not detect immunity in negative controls. (cdc.gov)
We decided to follow up with these volunteers by repeating antibody tests and by assessing T-cell immunity by enzyme-linked immunospot (ELISpot) assay for interferon-γ (IFN-γ). (cdc.gov)
The final antibody pair used in the REGN-COV2 therapy cocktail was then produced in Chinese hamster ovary (CHO) cells . (illinoisreview.com)

Asp32~Lys103 (Accession # D3ZI84)) fused with N-terminal His tag was produced in E. coli cells. (creativebiomart.net)

Expressed in HEK293 and purified by affinity chromatography. (proteomecommons.org)

Gene and cell therapy workflows frequently utilize HEK293/293T cells in the production of viral vectors. (cellandgene.com)

Evaluated by Immunocytochemistry in HeLa cells. (sigmaaldrich.com)
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected DNA-RNA hybrid in HeLa cells. (sigmaaldrich.com)
To provide insight into our understanding of the expression of the ORMDL3 gene, we isolated and characterized a splicing isoform of ORMDL3, ORMDL3 V1, from Hela cells by 5'and 3'-rapid amplification of cDNA end analysis (RACE) and RT-PCR. (curehunter.com)
Description: GFP expression stable cell line in Hela cells with Blasticidin marker. (choleratox.com)

Such complexes were isolated from lysates of HEK293 cells by chromatography on heparin-Sepharose. (uthscsa.edu)
Total cell lysates after three freeze-thaw cycles were 10-fold serially diluted in Gibco™ DMEM medium (Thermo Fisher Scientific, Cat. (cellculturedish.com)
Diluted cell lysates (200 µL) were inoculated into a 96-well plate seeded with AD-293 cells (Agilent, Cat. (cellculturedish.com)

Overall extracts from stably transfected HEK 293 cells expressing four different 7TM-variants of hMOR-1 have been made from 50? (dhfrinhibitor.com)
The Alt-R CRISPR-Cas9 RNA oligonucleotides (30 nM) were introduced by lipofection into HEK-293 cells that stably express Streptococcus pyogenes Cas9. (idtdna.com)
The uptake activity was measured in HEK293 cell-lines stably overexpressing OATP1B1⁄1a, OATP1B3 or OATP2B1, the major transporters involved in human hepatic uptake of rosuvastatin, or expressing OATP1B1⁄15, associated with reduced hepatic uptake of rosuvastatin. (tno.nl)

We generated a collection of Mst1 deletion mutants subcloned into pCS26MT vector with 66 Myc tag and transfected these mutants into HEK293 cells along with FlagGAPDH. (adenosine-receptor.com)

Luciferase from the sea pansy Renilla reniformis is often used in multiplexed luciferase assays as a second reporter to normalize for transfection efficiency, cell viability, and other factors that may vary among assay samples. (moleculardevices.com)
The HEPES transfection did not work in HEK293, Neuro-2a, N38 and SH-SH5Y cells, with no discernible amount of GFP detected on a fluorescent microscope in all four cell lines (data not shown). (openlabnotebooks.org)

View cart "Luciferase (firefly) & CRE Expression stable cell line (Puro)" has been added to your cart. (gentarget.com)
Description: Luciferase (firefry) stable cell line with Blasticidin marker. (choleratox.com)
Down-regulation of UL138 expression by hcmv-miR-UL36 was validated using luciferase reporter assays and Western blot analysis in HEK293 cells. (who.int)

They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility, as well as synthesis and deposition of the extracellular matrix. (peprotech.com)
Determined by its ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells. (peprotech.com)
[2] Nebivolol reduces cell proliferation of human coronary smooth muscle cells (haCSMCs) and endothelial cells (haECs) in a concentration- and time-dependent maner. (selleckchem.com)
Nebivolol treatment for 7 days causes significant reduction in cell growth of haCSMCs with IC50 of 6.1 μM, and inhibits accelerated haCSMC proliferation stimulated by growth factors PDGF-BB, bFGF, and TGFβ with IC50 values of 6.8 μM, 6.4 μM and 7.7 μM, repectively. (selleckchem.com)
B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. (prosci-inc.com)
Epithelial cells in tumor tissue expressed VEGFR2 and responded to VEGF stimulation with augmented VEGFR2-mediated proliferation. (perkinelmer.com)
Blockade of VEGF function via soluble decoy receptors suppressed tumor development, inhibited tumor angiogenesis, and blocked tumor cell proliferation. (perkinelmer.com)
Studies in conditional STAT3 mutant mice showed that VEGFR signaling requires STAT3 to promote epithelial cell proliferation and tumor growth in vivo. (perkinelmer.com)

CD100 stimulates the migration of cerebellar granule cells and of endothelial cells, regulates dendrite and axon branching and morphogenesis. (prospecbio.com)

Are aborted fetal cells added to artificial flavors in food products? (newsfactsnetwork.com)

B, Mst1 immunoprecipitated from HEK293 cells transfected with possibly Myc-Mst1 or Myc-Mst1 as well as Flag-GAPDH expression vectors was incubated with two mg MBP for the in vitro kinase assay. (adenosine-receptor.com)
Luciferases are the most common reporter genes, as they are easily detectable using luminometers or luminescence microplate readers, and the low luminescent background present in cells enables high assay sensitivity. (moleculardevices.com)
The T7 endonuclease I (T7EI) mismatch cleavage assay detects on-target genome editing and estimates genome editing efficiency in CRISPR-treated cells. (idtdna.com)
A cell-based assay (CBA) with GluK2-expressing HEK293 cells was used to assess 596 patients with different neurological disorders, and 23 healthy controls. (bvsalud.org)

HEK293SF-3F6 is a suspension-adapted cell line that has successfully been used for the production of the influenza virus, virus-like particles, lentivirus vectors, adenovirus and adeno-associated virus. (pharmaceutical-business-review.com)

Figure 2 - Validation of the expression of SARS-CoV-2 spike by 293-SARS2-S‑dfur cells. (invivogen.com)
In addition, their cell size, morphology and division rate, and low expression of native channels are traits that are particularly attractive for current-voltage measurements. (edu.sa)
To determine whether or not the interaction among Mst1 and GAPDH happens in mammalian cells, coimmunoprecipitation experiments were performed in HEK293T cells transfected with GAPDH and Mst1 expression vectors. (adenosine-receptor.com)
CD100 Human Recombinant produced by mammalian expression system in human cells is a single polypeptide chain containing 721 amino acids (22-734). (prospecbio.com)
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. (choleratox.com)
In the end, I decided to go back to the BacMAM system in SH-SY5Y cells and spend some time tweaking the cell culture media and timing of the transduction to improve BacMAM HTT expression. (openlabnotebooks.org)
Overall, I observe that there is a trade-off between increased HTT expression and cell death with higher v/v BacMAM virus levels or longer transduction times. (openlabnotebooks.org)
Moving forward, I will need to optimize these parameters to get the highest HTT expression with lowest SH-SY5Y cell death. (openlabnotebooks.org)

The cell-type-specific effect of gene perturbation is extremely interesting and explains why the neuron is the cell most vulnerable to oxidative stress during neurodegeneration. (alzforum.org)

with a functional furin cleavage site and recommended for cell-cell fusion assays with ACE2-expressing cells. (invivogen.com)
To this end, cell-based assays using Spike-expressing cells provide a more relevant in vivo biological context than biochemical assays (e.g. (invivogen.com)
Functionally validated in cell fusion assays with HEK-Blue™ hACE2(-TMPRSS2) Cells (293-SARS2-S Cells) and by flow cytometry (293-SARS2-S-dfur Cells). (invivogen.com)
Cell fusion assays for screening novel inhibitors of the SARS-CoV-2 Spike-host interaction using InvivoGen's permissive reporter cell lines ( HEK-Blue™ hACE2(-TMPRSS2) and A549-Dual™ hACE2-TMPRSS2 ). (invivogen.com)
To estimate editing efficiency, genomic DNA samples from the transfected cells were tested using the Alt-R Genome Editing Detection Kit (dark blue bars), which provides reagents needed to run T7EI assays. (idtdna.com)

The Blasticidin Selection J774Cells reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (choleratox.com)

The facility has ample experience with production of monoclonal antibodies from hybridoma cell lines. (nki.nl)
The system was validated with a random panel of 45 whole-cell culture broths containing recombinant antibodies in the early profiling phase. (cyberleninka.org)
Using interferon-γ ELISpot, we observed that 78% of PCR-positive volunteers with undetectable antibodies showed T cell immunity against SARS-CoV-2. (cdc.gov)

The cell line can be cultured in an in-house media and in commercially-available serum-free media but weak growth profiles are obtained in chemically defined media. (pharmaceutical-business-review.com)
Please note: Each cell line is sold separately. (invivogen.com)
See TDS for the exact contents of each cell line. (invivogen.com)
This HEK293-hNRP1-hACE2 stable cell line expresses a recombinant human Neuropilin-1 (NRP-1) and a human Angiotensin-Converting Enzyme 2 (ACE2). (eenzyme.com)
In this paper we evaluated two technologies, both already well accepted in CHO-based cell line development workflows, for their suitability with HEK cells. (cellandgene.com)

Together, these findings show that GAPDH and Mst1 exist in the same complicated in mammalian cells both at baseline situations and during apoptotic pressure. (adenosine-receptor.com)
Mammalian cells (HEK293 and derivatives). (nki.nl)

Methods: Cultured HEK293 cells were transiently transfected with hERG channel cDNA plasmid pcDNA3.1 using Lipofectamine. (chinaphar.com)

Furthermore, HEK293 reporter cell lines were employed to evaluate the role of Toll-like receptor activation. (cdc.gov)

Individual recombinant viral isolates are plucked from agarose and used to infect Sf9 insect cells. (bcm.edu)

The data show that, at concentrations currently employed as Ca2+ channel blockers, some of these compounds also inhibit certain subtypes of Nicotinic AChR currents, and calls for caution when interpreting many of the results obtained in neurons and other cell types, where nicotinic receptor and Ca2- channels coexist. (semanticscholar.org)
CD100 is the cell surface receptor forPLXN1B and PLXNB2 which plays a central role in cell-cell signaling. (prospecbio.com)
B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. (prosci-inc.com)
Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. (dimabio.com)
Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. (dimabio.com)

Using THP-1 knockdown cell lines, we found that IL-1beta secretion was caspase-1-, ASC-, and NLRP3- dependent. (cdc.gov)

Ryan splits his time between teaching medical genomics and diagnostic technologies, researching cell biology and its changes in disease, commercial spinout and building links in medical research across Oxford. (brookes.ac.uk)
Ryan enjoys teaching cell and molecular biology, disease pathology, genomic technologies and the use of digital technologies in diagnostics. (brookes.ac.uk)

20 4 several hours soon after seeding, HEK 293 cells were transfected making use of FuGene High definition (Roche) and GFP-tagged hMOR1AD (m3-like) (one mg plasmid DNA/dish). (dhfrinhibitor.com)

The stability for 20 passages, following thawing, has been verified for both cell lines. (invivogen.com)
Read how the use of VIPS and InstiGRO HEK were found to be a strong combination for developing HEK based cell lines whilst delivering on the regulatory demands for clonally-derived Master Cell Banks. (cellandgene.com)
Hybridoma cell lines have to be provided by the applicant (external projects) or should be accessible from the NKI hybridoma bank (For NKI researchers only! (nki.nl)
Since then, I have attempted to use the BacMAM system in two other neuronal cell lines, Neuro-2a and N38. (openlabnotebooks.org)
In contrast to primary hepatocytes, estimating carrier-mediated hepatic disposition by using a panel of single transfected cell-lines provides direct information on the contribution of the individual transporters to the net disposition. (tno.nl)
The ABL1 inhibitors imatinib and nilotinib cluster collectively because they selectively inhibit the cell lines A-204 and K-562 that are reliant on ABL1 for development (Number 2C). (conferencedequebec.org)
However, additional kinase medicines inhibit the development of multiple cell lines, such axitinib, ponatinib, bosutinib, sunitinib and crizotinib, which cluster collectively in heat map (Number 2C), the mTOR inhibitors temsirolimus and everolimus, as well as the MEK inhibitor trametinib (Number 2C). (conferencedequebec.org)

An excisional wound on the mouse back was contaminated with one of two bioluminescent Gram-negative species, Proteus mirabilis (2.5 x 10(7) cells) and Pseudomonas aeruginosa (5 x 10(6) cells). (perkinelmer.com)

Insect cells are co-transfected with wild type Autographa californica nuclear polyhedrosis virus DNA (AcNPV) and baculovirus transfer plasmids and isolates recombinant baculoviruses that arise by homologous recombination. (bcm.edu)

Cell and tissue research (Cell Tissue Res) Vol. 346 Issue 2 Pg. (curehunter.com)
B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. (prosci-inc.com)

In my last blog post , I described how I am trying to overexpress HTT in neuronal cells. (openlabnotebooks.org)
While the BacMAM system was easy to implement in cells such as HEK293, it proved far more challenging to express in SH-SH5Y neuronal cells. (openlabnotebooks.org)

The aim of this study was to investigate the effects of ALL and BTHP on the human ether-a-go-go related gene (hERG) current expressed in HEK293 cells. (chinaphar.com)
Not yet a member of Cell & Gene? (cellandgene.com)

Description: CHO Host Cell DNA Detection Kit in tubes by Cygnus Technologies is available in Europe via Gentaur. (gentaurtop.com)

Thus, VEGFR-signaling acts as a direct growth factor for tumor cells in CAC, providing a molecular link between inflammation and the development of colon cancer. (perkinelmer.com)

Stimulates perforin-mediated elimination of ligand-expressing tumor cells. (dimabio.com)

May play a regulatory role in differentiation and survival of NK cells. (dimabio.com)

Suspension HEK293F cells (Invitrogen) grown in serum-free 293 Freestyle medium (Invitrogen). (glyomics.org)
As a model system, we infected suspension HEK293 cells with wildtype AdV serotype 5 to assess AdV production using Gibco™ Dynamis™ Medium with Gibco™ EfficientFeed™ C+ (EFC+). (cellculturedish.com)
Growth performance was evaluated in simple fed-batch conditions with suspension HEK293 cells in Dynamis Medium (Thermo Fisher Scientific, Cat. (cellculturedish.com)
Suspension HEK293 cells were adapted to Dynamis Medium or Medium A (alternative commercially available medium) with 10 mg/mL insulin for a minimum of 3 passages. (cellculturedish.com)
Suspension HEK293 cells cultured in Dynamis Medium showed increasing VCD for more than 7 days. (cellculturedish.com)

They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. (peprotech.com)

Anatomy8

Organisms1

Chemicals and Drugs39

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes26

Disciplines and Occupations1

Information Science3

Mammalian Notum induces the release of glypicans and other GPI-anchored proteins from the cell surface. (1/10093)

alpha-Actinin interacts with rapsyn in agrin-stimulated AChR clustering. (2/10093)

Influence of brain-derived neurotrophic factor on pathfinding of dentate granule cell axons, the hippocampal mossy fibers. (3/10093)

Effective gene-viral therapy of leukemia by a new fiber chimeric oncolytic adenovirus expressing TRAIL: in vitro and in vivo evaluation. (4/10093)

A novel human monoclonal antibody that binds with high affinity to mesothelin-expressing cells and kills them by antibody-dependent cell-mediated cytotoxicity. (5/10093)

Regulation of dopamine transporter activity by carboxypeptidase E. (6/10093)

Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models. (7/10093)

Selenoprotein P regulation by the glucocorticoid receptor. (8/10093)

HEK293 Cells

Cell Line

Transfection

Kidney

Receptor, EphA3

Patch-Clamp Techniques

Molecular Sequence Data

Protein Binding

Recombinant Proteins

Amino Acid Sequence

Cell Membrane

Protein Structure, Tertiary

Calcium

Cells, Cultured

Protein Transport

Signal Transduction

Mutation

Ion Channel Gating

TRPC Cation Channels

Phosphorylation

Recombinant Fusion Proteins

DNA, Complementary

Membrane Potentials

Blotting, Western

Immunoprecipitation

Arrestins

Dose-Response Relationship, Drug

Microscopy, Confocal

Green Fluorescent Proteins

Electrophysiology

Receptors, G-Protein-Coupled

Cloning, Molecular

Protein Isoforms

Kinetics

Membrane Proteins

Radioligand Assay

Ether-A-Go-Go Potassium Channels

Gene Expression Regulation

Cyclic AMP

Mutagenesis, Site-Directed

Base Sequence

Enzyme Activation

Calcium Channels

TRPV Cation Channels

Receptors, Purinergic P2X7

Gene Expression

RNA, Small Interfering

Potassium Channels, Inwardly Rectifying

Binding Sites

Luminescent Proteins

RNA, Messenger

Ligands

Endocytosis

Organic Cation Transport Proteins

Receptors, Calcium-Sensing

Two-Hybrid System Techniques

Potassium Channels

Protein Subunits

Microscopy, Fluorescence

Sodium Channels

Fluorescence Resonance Energy Transfer

Receptor, EphB3

Glycosylation

Sequence Homology, Amino Acid

COS Cells

Receptors, Serotonin, 5-HT3

TRPM Cation Channels

NAV1.5 Voltage-Gated Sodium Channel

Biotinylation

Potassium Channels, Voltage-Gated

Cyclic AMP-Dependent Protein Kinases

Amino Acid Substitution

Reverse Transcriptase Polymerase Chain Reaction

GTP-Binding Protein alpha Subunits, Gq-G11

Lymphocyte Antigen 96

CHO Cells

Calcium Signaling

GTP-Binding Proteins

Mutation, Missense

Kv1.5 Potassium Channel

$How to treat HEK293 with S9 fraction?: post #1$ How to treat HEK293 with S9 fraction?: post #1