Histone Deacetylases: Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes.Histone Deacetylase 1: A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 2; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.Histone Deacetylase 2: A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 1; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.Histone Deacetylase Inhibitors: Compounds that inhibit HISTONE DEACETYLASES. This class of drugs may influence gene expression by increasing the level of acetylated HISTONES in specific CHROMATIN domains.Hydroxamic Acids: A class of weak acids with the general formula R-CONHOH.Acetylation: Formation of an acetyl derivative. (Stedman, 25th ed)Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.Valproic Acid: A fatty acid with anticonvulsant properties used in the treatment of epilepsy. The mechanisms of its therapeutic actions are not well understood. It may act by increasing GAMMA-AMINOBUTYRIC ACID levels in the brain or by altering the properties of voltage dependent sodium channels.MEF2 Transcription Factors: Activating transcription factors of the MADS family which bind a specific sequence element (MEF2 element) in many muscle-specific genes and are involved in skeletal and cardiac myogenesis, neuronal differentiation and survival/apoptosis.Myogenic Regulatory Factors: A family of muscle-specific transcription factors which bind to DNA in control regions and thus regulate myogenesis. All members of this family contain a conserved helix-loop-helix motif which is homologous to the myc family proteins. These factors are only found in skeletal muscle. Members include the myoD protein (MYOD PROTEIN); MYOGENIN; myf-5, and myf-6 (also called MRF4 or herculin).Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Sin3 Histone Deacetylase and Corepressor Complex: A multisubunit enzyme complex that regulates GENETIC TRANSCRIPTION by deacetylating the HISTONE residues of NUCLEOSOMES.Cell Line, Tumor: A cell line derived from cultured tumor cells.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Nuclear Receptor Co-Repressor 2: A nuclear co-repressor protein that shows specificity for RETINOIC ACID RECEPTORS and THYROID HORMONE RECEPTORS. The dissociation of this co-repressor from nuclear receptors is generally ligand-dependent, but can also occur by way of its phosphorylation by members of the MAP KINASE SIGNALING SYSTEM. The protein contains two nuclear receptor interaction domains and four repressor domains and is closely-related in structure to NUCLEAR RECEPTOR CO-REPRESSOR 1.Butyrates: Derivatives of BUTYRIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxypropane structure.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Depsipeptides: Compounds consisting of chains of AMINO ACIDS alternating with CARBOXYLIC ACIDS via ester and amide linkages. They are commonly cyclized.Histone Acetyltransferases: Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Epigenesis, Genetic: A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.p300-CBP Transcription Factors: A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.Active Transport, Cell Nucleus: Gated transport mechanisms by which proteins or RNA are moved across the NUCLEAR MEMBRANE.Mi-2 Nucleosome Remodeling and Deacetylase Complex: A enzyme complex involved in the remodeling of NUCLEOSOMES. The complex is comprised of at least seven subunits and includes both histone deacetylase and ATPase activities.Chromatin: The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Tubulin: A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.Co-Repressor Proteins: A subclass of repressor proteins that do not directly bind DNA. Instead, co-repressors generally act via their interaction with DNA-BINDING PROTEINS such as a TRANSCRIPTIONAL SILENCING FACTORS or NUCLEAR RECEPTORS.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Gene Silencing: Interruption or suppression of the expression of a gene at transcriptional or translational levels.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Cyclin-Dependent Kinase Inhibitor p21: A cyclin-dependent kinase inhibitor that mediates TUMOR SUPPRESSOR PROTEIN P53-dependent CELL CYCLE arrest. p21 interacts with a range of CYCLIN-DEPENDENT KINASES and associates with PROLIFERATING CELL NUCLEAR ANTIGEN and CASPASE 3.RNA Interference: A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.HCT116 Cells: Human COLORECTAL CARCINOMA cell line.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.AnilidesTranscriptional Activation: Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.Immunoprecipitation: The aggregation of soluble ANTIGENS with ANTIBODIES, alone or with antibody binding factors such as ANTI-ANTIBODIES or STAPHYLOCOCCAL PROTEIN A, into complexes large enough to fall out of solution.Nuclear Receptor Subfamily 4, Group A, Member 1: An orphan nuclear receptor that is closely related to members of the thyroid-steroid receptor gene family. It was originally identified in NERVE CELLS and may play a role in mediation of NERVE GROWTH FACTOR-induced CELL DIFFERENTIATION. However, several other functions have been attributed to this protein including the positive and negative regulation of APOPTOSIS.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Benzamides: BENZOIC ACID amides.HEK293 Cells: A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.Gene Knockdown Techniques: The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Chromatin Assembly and Disassembly: The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA.Sp1 Transcription Factor: Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.NIH 3T3 Cells: A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Lysine: An essential amino acid. It is often added to animal feed.Mice, Inbred C57BLRNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.MADS Domain Proteins: A superfamily of proteins that share a highly conserved MADS domain sequence motif. The term MADS refers to the first four members which were MCM1 PROTEIN; AGAMOUS 1 PROTEIN; DEFICIENS PROTEIN; and SERUM RESPONSE FACTOR. Many MADS domain proteins have been found in species from all eukaryotic kingdoms. They play an important role in development, especially in plants where they have an important role in flower development.Cytoplasm: The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Antineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.E1A-Associated p300 Protein: A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.Protein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.HSP90 Heat-Shock Proteins: A class of MOLECULAR CHAPERONES whose members act in the mechanism of SIGNAL TRANSDUCTION by STEROID RECEPTORS.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Acetyltransferases: Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.COS Cells: CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)Genes, Reporter: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.Calcium-Calmodulin-Dependent Protein Kinase Type 1: A monomeric calcium-calmodulin-dependent protein kinase subtype that is expressed in a broad variety of mammalian cell types. Its expression is regulated by the action of CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE. Several isoforms of this enzyme subtype are encoded by distinct genes.Isothiocyanates: Organic compounds with the general formula R-NCS.CREB-Binding Protein: A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.Cell Cycle Proteins: Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.DNA (Cytosine-5-)-Methyltransferase: An enzyme that catalyzes the transfer of a methyl group from S-ADENOSYLMETHIONINE to the 5-position of CYTOSINE residues in DNA.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Luciferases: Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.YY1 Transcription Factor: A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. It interacts with key regulatory proteins such as TATA-BINDING PROTEIN; TFIIB; and ADENOVIRUS E1A PROTEINS.Pimelic Acids: A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.SUMO-1 Protein: A 1.5-kDa small ubiquitin-related modifier protein that can covalently bind via an isopeptide link to a number of cellular proteins. It may play a role in intracellular protein transport and a number of other cellular processes.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Indoles: Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.Drug Synergism: The action of a drug in promoting or enhancing the effectiveness of another drug.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Proteasome Endopeptidase Complex: A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.Tumor Suppressor Protein p53: Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Pyridines: Compounds with a six membered aromatic ring containing NITROGEN. The saturated version is PIPERIDINES.Histone Demethylases: Enzymes that catalyse the removal of methyl groups from LYSINE or ARGININE residues found on HISTONES. Many histone demethylases generally function through an oxidoreductive mechanism.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Thiocyanates: Organic derivatives of thiocyanic acid which contain the general formula R-SCN.Liver Extracts: Extracts of liver tissue containing uncharacterized specific factors with specific activities; a soluble thermostable fraction of mammalian liver is used in the treatment of pernicious anemia.DNA Methylation: Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.Precipitin Tests: Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.Gene Expression Regulation, Developmental: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.Xenograft Model Antitumor Assays: In vivo methods of screening investigative anticancer drugs, biologic response modifiers or radiotherapies. Human tumor tissue or cells are transplanted into mice or rats followed by tumor treatment regimens. A variety of outcomes are monitored to assess antitumor effectiveness.Proteasome Inhibitors: Compounds that inhibit the function or proteolytic action of the PROTEASOME.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Real-Time Polymerase Chain Reaction: Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.Transcription Factor RelA: A subunit of NF-kappa B that is primarily responsible for its transactivation function. It contains a C-terminal transactivation domain and an N-terminal domain with homology to PROTO-ONCOGENE PROTEINS C-REL.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Two-Hybrid System Techniques: Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.Core Binding Factor Alpha 1 Subunit: A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.Sirtuin 1: A sirtuin family member found primarily in the CELL NUCLEUS. It is an NAD-dependent deacetylase with specificity towards HISTONES and a variety of proteins involved in gene regulation.Somatostatin-Secreting Cells: Endocrine cells found throughout the GASTROINTESTINAL TRACT and in islets of the PANCREAS. D cells secrete SOMATOSTATIN that acts in both an endocrine and paracrine manner. Somatostatin acts on a variety of tissues including the PITUITARY GLAND; gastrointestinal tract; pancreas; and KIDNEY by inhibiting the release of hormones, such as GROWTH HORMONE; GASTRIN; INSULIN; and RENIN.Proteolysis: Cleavage of proteins into smaller peptides or amino acids either by PROTEASES or non-enzymatically (e.g., Hydrolysis). It does not include Protein Processing, Post-Translational.Ubiquitin: A highly conserved 76-amino acid peptide universally found in eukaryotic cells that functions as a marker for intracellular PROTEIN TRANSPORT and degradation. Ubiquitin becomes activated through a series of complicated steps and forms an isopeptide bond to lysine residues of specific proteins within the cell. These "ubiquitinated" proteins can be recognized and degraded by proteosomes or be transported to specific compartments within the cell.Cortactin: A microfilament protein that interacts with F-ACTIN and regulates cortical actin assembly and organization. It is also an SH3 DOMAIN containing phosphoprotein, and it mediates tyrosine PHOSPHORYLATION based SIGNAL TRANSDUCTION by PROTO-ONCOGENE PROTEIN PP60(C-SRC).Cerebellar Cortex: The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Huntington Disease: A familial disorder inherited as an autosomal dominant trait and characterized by the onset of progressive CHOREA and DEMENTIA in the fourth or fifth decade of life. Common initial manifestations include paranoia; poor impulse control; DEPRESSION; HALLUCINATIONS; and DELUSIONS. Eventually intellectual impairment; loss of fine motor control; ATHETOSIS; and diffuse chorea involving axial and limb musculature develops, leading to a vegetative state within 10-15 years of disease onset. The juvenile variant has a more fulminant course including SEIZURES; ATAXIA; dementia; and chorea. (From Adams et al., Principles of Neurology, 6th ed, pp1060-4)Methylation: Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)Multiprotein Complexes: Macromolecular complexes formed from the association of defined protein subunits.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Brachydactyly: Congenital anomaly of abnormally short fingers or toes.Cardiomegaly: Enlargement of the HEART, usually indicated by a cardiothoracic ratio above 0.50. Heart enlargement may involve the right, the left, or both HEART VENTRICLES or HEART ATRIA. Cardiomegaly is a nonspecific symptom seen in patients with chronic systolic heart failure (HEART FAILURE) or several forms of CARDIOMYOPATHIES.Proto-Oncogene Proteins c-myc: Cellular DNA-binding proteins encoded by the c-myc genes. They are normally involved in nucleic acid metabolism and in mediating the cellular response to growth factors. Elevated and deregulated (constitutive) expression of c-myc proteins can cause tumorigenesis.Microtubules: Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Cell Death: The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.Matrix Metalloproteinase 10: A secreted matrix metalloproteinase that may play a role in matrix degradation during WOUND HEALING. It is expressed at high levels by KERATINOCYTES, suggesting its role in keratinocyte migration.CCAAT-Binding Factor: A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.HT29 Cells: Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.Inhibitory Concentration 50: The concentration of a compound needed to reduce population growth of organisms, including eukaryotic cells, by 50% in vitro. Though often expressed to denote in vitro antibacterial activity, it is also used as a benchmark for cytotoxicity to eukaryotic cells in culture.Immunoblotting: Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Serotonergic Neurons: Neurons whose primary neurotransmitter is SEROTONIN.Histone-Lysine N-Methyltransferase: An enzyme that catalyzes the methylation of the epsilon-amino group of lysine residues in proteins to yield epsilon mono-, di-, and trimethyllysine. EC 188.8.131.52.Memory, Long-Term: Remembrance of information from 3 or more years previously.Drug Screening Assays, Antitumor: Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals.Retinoblastoma Protein: Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.Cercopithecus aethiops: A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.Catalytic Domain: The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.Response Elements: Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.Peptides, Cyclic: Peptides whose amino and carboxy ends are linked together with a peptide bond forming a circular chain. Some of them are ANTI-INFECTIVE AGENTS. Some of them are biosynthesized non-ribosomally (PEPTIDE BIOSYNTHESIS, NON-RIBOSOMAL).Myocytes, Cardiac: Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).Embryo Loss: Early pregnancy loss during the EMBRYO, MAMMALIAN stage of development. In the human, this period comprises the second through eighth week after fertilization.K562 Cells: An ERYTHROLEUKEMIA cell line derived from a CHRONIC MYELOID LEUKEMIA patient in BLAST CRISIS.Calcium-Calmodulin-Dependent Protein Kinase Type 2: A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. It differs from other enzyme subtypes in that it lacks a phosphorylatable activation domain that can respond to CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE.Azacitidine: A pyrimidine analogue that inhibits DNA methyltransferase, impairing DNA methylation. It is also an antimetabolite of cytidine, incorporated primarily into RNA. Azacytidine has been used as an antineoplastic agent.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Autophagy: The segregation and degradation of damaged or unwanted cytoplasmic constituents by autophagic vacuoles (cytolysosomes) composed of LYSOSOMES containing cellular components in the process of digestion; it plays an important role in BIOLOGICAL METAMORPHOSIS of amphibians, in the removal of bone by osteoclasts, and in the degradation of normal cell components in nutritional deficiency states.Butyric Acid: A four carbon acid, CH3CH2CH2COOH, with an unpleasant odor that occurs in butter and animal fat as the glycerol ester.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Kruppel-Like Transcription Factors: A family of zinc finger transcription factors that share homology with Kruppel protein, Drosophila. They contain a highly conserved seven amino acid spacer sequence in between their ZINC FINGER MOTIFS.NF-kappa B: Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.Chromosomal Proteins, Non-Histone: Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.Sirtuins: A homologous family of regulatory enzymes that are structurally related to the protein silent mating type information regulator 2 (Sir2) found in Saccharomyces cerevisiae. Sirtuins contain a central catalytic core region which binds NAD. Several of the sirtuins utilize NAD to deacetylate proteins such as HISTONES and are categorized as GROUP III HISTONE DEACETYLASES. Several other sirtuin members utilize NAD to transfer ADP-RIBOSE to proteins and are categorized as MONO ADP-RIBOSE TRANSFERASES, while a third group of sirtuins appears to have both deacetylase and ADP ribose transferase activities.Receptors, Glucocorticoid: Cytoplasmic proteins that specifically bind glucocorticoids and mediate their cellular effects. The glucocorticoid receptor-glucocorticoid complex acts in the nucleus to induce transcription of DNA. Glucocorticoids were named for their actions on blood glucose concentration, but they have equally important effects on protein and fat metabolism. Cortisol is the most important example.Protein Isoforms: Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.PhenylenediaminesEmbryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Receptors, Cytoplasmic and Nuclear: Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.Jurkat Cells: A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.Homeodomain Proteins: Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).Leupeptins: A group of acylated oligopeptides produced by Actinomycetes that function as protease inhibitors. They have been known to inhibit to varying degrees trypsin, plasmin, KALLIKREINS, papain and the cathepsins.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Teaching Rounds: Systematic discussions and teaching relating to patient care.Growth Differentiation Factors: A family of BONE MORPHOGENETIC PROTEIN-related proteins that are primarily involved in regulation of CELL DIFFERENTIATION.Tumor Suppressor Proteins: Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.Nerve Tissue ProteinsCarrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Cell Cycle Checkpoints: Regulatory signaling systems that control the progression through the CELL CYCLE. They ensure that the cell has completed, in the correct order and without mistakes, all the processes required to replicate the GENOME and CYTOPLASM, and divide them equally between two daughter cells. If cells sense they have not completed these processes or that the environment does not have the nutrients and growth hormones in place to proceed, then the cells are restrained (or "arrested") until the processes are completed and growth conditions are suitable.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Protein Stability: The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Friedreich Ataxia: An autosomal recessive disease, usually of childhood onset, characterized pathologically by degeneration of the spinocerebellar tracts, posterior columns, and to a lesser extent the corticospinal tracts. Clinical manifestations include GAIT ATAXIA, pes cavus, speech impairment, lateral curvature of spine, rhythmic head tremor, kyphoscoliosis, congestive heart failure (secondary to a cardiomyopathy), and lower extremity weakness. Most forms of this condition are associated with a mutation in a gene on chromosome 9, at band q13, which codes for the mitochondrial protein frataxin. (From Adams et al., Principles of Neurology, 6th ed, p1081; N Engl J Med 1996 Oct 17;335(16):1169-75) The severity of Friedreich ataxia associated with expansion of GAA repeats in the first intron of the frataxin gene correlates with the number of trinucleotide repeats. (From Durr et al, N Engl J Med 1996 Oct 17;335(16):1169-75)Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Sumoylation: A type of POST-TRANSLATIONAL PROTEIN MODIFICATION by SMALL UBIQUITIN-RELATED MODIFIER PROTEINS (also known as SUMO proteins).DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.Nuclear Receptor Subfamily 4, Group A, Member 2: An orphan nuclear receptor that is found at high levels in BRAIN tissue. The protein is believed to play a role in development and maintenance of NEURONS, particularly dopaminergic neurons.Ubiquitinated Proteins: Proteins covalently modified with UBIQUITINS or UBIQUITIN-LIKE PROTEINS.Animals, Outbred Strains: Animals that are generated from breeding two genetically dissimilar strains of the same species.Glutathione Transferase: A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.E2F Transcription Factors: A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
"SLy2 targets the nuclear SAP30/HDAC1 complex". Int. J. Biochem. Cell Biol. 42 (9): 1472-81. doi:10.1016/j.biocel.2010.05.004. ...
HDAC1 (1p35). *HES2: Hes family bHLH transcription factor 2. *HES3: Hes family bHLH transcription factor 3 ...
"MDM2-HDAC1-mediated deacetylation of p53 is required for its degradation". The EMBO Journal. 21 (22): 6236-45. doi:10.1093/ ...
... has been shown to interact with HDAC1. GRCh38: Ensembl release 89: ENSG00000198160 - Ensembl, May 2017 GRCm38: Ensembl ...
... has been shown to interact with HDAC1. GRCh38: Ensembl release 89: ENSG00000142182 - Ensembl, May 2017 GRCm38: Ensembl ...
Not even upregulation of the other Class I HDACs could compensate for the loss of HDAC1. This inability to recover from HDAC1 ... HDAC1 & HDAC2 are in the first class of HDACs are most closely related to one another. By analyzing the overall sequences of ... In HDAC1 knockout (KO) mice, mice were found to die during embryogenesis and showed a drastic reduction in the production but ... Targets of Vorinostat includes HDAC1, HDAC2, HDAC3 and HDAC6. Suggested by the idea that the structure of chromatin can be ...
LTP was not altered between HDAC1 KO and OE mice. HDAC2 suppresses neuronal gene expression. HDAC2 interacted more than HDAC1 ... mice while HDAC1 displayed similar freezing behaviors to WTs. In summary, Guan et al. have shown that: HDAC2, not HDAC1, ... Overexpression (OE) of HDAC1 and HDAC2 in mice resulted in decreased levels of acetylated lysines. After exposing these mice to ... CoREST, a co-repressor, associates with HDAC2 not HDAC1. SAHA, a HDAC inhibitor, increased freezing of HDAC2 OE mice in ...
Rb interacts with the histone deactylases HDAC1, HDAC3, and HDAC3. Rb binds to HDAC1 in its pocket domain in a region that is ... Mal A, Sturniolo M, Schiltz RL, Ghosh MK, Harter ML (April 2001). "A role for histone deacetylase HDAC1 in modulating the ... The DREAM complex exists in quiescent cells in association with MuvB (consisting of HDAC1 or HDAC2, LIN52 and L3mbtl1, L3mbtl3 ... E2F1 and HDAC1 and represses transcription from E2F-responsive promoters". Nat. Genet. 25 (3): 338-42. doi:10.1038/77124. PMID ...
and inhibition of p300/CBP induced histone acetylation (INHAT). JDP2 recruits histone deacetylases HDAC1 and HDAC2, HDAC6 and ...
... is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex ( ...
SDS3 is a subunit of the histone deacetylase (see HDAC1; MIM 601241)-dependent SIN3A (MIM 607776) corepressor complex ( ... Fleischer et al., 2003).[supplied by OMIM] SUDS3 has been shown to interact with HDAC1, Host cell factor C1, SIN3B and SIN3A. ...
HDAC1 negatively regulates the cardiovascular transcription factor Kruppel-like factor 5 through direct interaction. Estrogen ... Matsumura T, Suzuki T, Aizawa K, Munemasa Y, Muto S, Horikoshi M, Nagai R (April 2005). "The deacetylase HDAC1 negatively ... Quisinostat (JNJ-26481585) Started phase I clinical trials HBI-8000, (a benzamide HDI), inhibits Class I HDAC1, HDAC2, HDAC3, ... which includes HDAC1, -2, -3 and -8 are related to yeast RPD3 gene; Class IIA, which includes HDAC4, -5, -7 and -9; Class IIB - ...
"The p65 (RelA) subunit of NF-kappaB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively ... "A role for histone deacetylase activity in HDAC1-mediated transcriptional repression". Proc. Natl. Acad. Sci. U.S.A. 95 (7): ... HDAC1, HMG20B, HSPA4, Host cell factor C1, MTA1, MTA2, MXD1, Mad1, Methyl-CpG-binding domain protein 2, PHF21A, PPP1R8, RBBP4, ... "A role for histone deacetylase activity in HDAC1-mediated transcriptional repression". Proc. Natl. Acad. Sci. U.S.A. 95 (7): ...
Ito A, Kawaguchi Y, Lai CH, Kovacs JJ, Higashimoto Y, Appella E, Yao TP (November 2002). "MDM2-HDAC1-mediated deacetylation of ...
... has been shown to interact with: BRCA1, CREBBP, GATAD2B, HDAC1, HDAC2, HDAC3, MTA2, RB, SAP30, and SIN3A. GRCh38: Ensembl ... 1998). "A role for histone deacetylase activity in HDAC1-mediated transcriptional repression". Proc. Natl. Acad. Sci. U.S.A. 95 ... "A role for histone deacetylase activity in HDAC1-mediated transcriptional repression". Proc. Natl. Acad. Sci. U.S.A. 95 (7): ...
Ito A, Kawaguchi Y, Lai CH, Kovacs JJ, Higashimoto Y, Appella E, Yao TP (November 2002). "MDM2-HDAC1-mediated deacetylation of ... "The phosphorylation status of nuclear NF-kappa B determines its association with CBP/p300 or HDAC-1". Molecular Cell. 9 (3): ...
... has been shown to interact with: CABIN1 HBP1, HDAC1, HDAC9, Histone deacetylase 2, Host cell factor C1, IKZF1, ING1, ... "A role for histone deacetylase activity in HDAC1-mediated transcriptional repression". Proceedings of the National Academy of ...
"Quantitation of HDAC1 mRNA expression in invasive carcinoma of the breast*". Breast Cancer Research and Treatment. 94 (1): 11-6 ...
Embryonic stem cell proliferation was unaffected by the loss of either HDAC1 or HDAC2 but the differentiation of embryonic stem ... 2010) used HDAC knockout mice to demonstrate whether HDAC1 or HDAC2 was important for the embryonic stem cell differentiation. ... The continued culture of HDAC1 knockout embryonic stem cells showed that the embryoid bodies formed became irregular and ... It showed that just before or during gastrulation, embryonic stem cells lacking HDAC1 acquired visible developmental defects. ...
... has been shown to interact with: HDAC1, Histone deacetylase 2 and SERBP1. GRCh38: Ensembl release 89: ENSG00000170004 - ...
... has been shown to interact with HDAC1, HDAC2, HMG20B, REST and PHF21A. GRCh38: Ensembl release 89: ENSG00000089902 - ...
... has been shown to interact with BUB1B, HDAC1 and Histone deacetylase 2. Bub3 has been shown to form complexes with Mad1- ...
... has been shown to interact with: BRD4, HDAC1, PCNA, RELA and RFC3. GRCh38: Ensembl release 89: ENSG00000035928 - Ensembl, ... Anderson LA, Perkins ND (Aug 2002). "The large subunit of replication factor C interacts with the histone deacetylase, HDAC1". ... Anderson LA, Perkins ND (2002). "The large subunit of replication factor C interacts with the histone deacetylase, HDAC1". J. ...
Sakai H, Urano T, Ookata K, Kim MH, Hirai Y, Saito M, Nojima Y, Ishikawa F (2003). "MBD3 and HDAC1, two components of the NuRD ... MBD3 has been shown to interact with: AURKA, GATAD2B, HDAC1, MTA2, and MBD2. GRCh38: Ensembl release 89: ENSG00000071655 - ... "MBD3 and HDAC1, two components of the NuRD complex, are localized at Aurora-A-positive centrosomes in M phase". J. Biol. Chem. ... "The mCpG-binding domain of human MBD3 does not bind to mCpG but interacts with NuRD/Mi2 components HDAC1 and MTA2". J. Biol. ...
Anderson LA, Perkins ND (Aug 2002). "The large subunit of replication factor C interacts with the histone deacetylase, HDAC1". ...
Histone deacetylase 1 (HDAC1) is an enzyme that in humans is encoded by the HDAC1 gene. Histone acetylation and deacetylation, ... "Entrez Gene: HDAC1 histone deacetylase 1". "Salmonella infection data for Hdac1". Wellcome Trust Sanger Institute. "Citrobacter ... HDAC1 has been shown to interact with: Androgen receptor, BCL6, BTG2, BUB1B, BUB1, BUB3, CBFA2T3, CDC20, CDH1, CHD3, CHD4, COUP ... Sakai H, Urano T, Ookata K, Kim MH, Hirai Y, Saito M, Nojima Y, Ishikawa F (December 2002). "MBD3 and HDAC1, two components of ...
Anti-HDAC1 antibody (ab19845) has been cited in 38 publications. References for Human, Mouse, Rat in ChIP, ICC/IF, IHC, IHC-Fr ...
Active Recombinant human HDAC1 protein is a Baculovirus Full length protein 1 to 482 aa range, , 70% purity and validated in WB ... Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional ... The specific activity of HDAC1 (ab101661) was determined to be 10230 RLU/min/mg as per activity assay protocol ... Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. ...
Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Part of a complex containing at least CDYL, MIER1, MIER2, HDAC1 ... Component of a mSin3A corepressor complex that contains SIN3A, SAP130, SUDS3/SAP45, ARID4B/SAP180, HDAC1 and HDAC2. Found in a ... Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80 ... The large PER complex involved in the histone deacetylation is composed of at least HDAC1, PER2, SFPQ and SIN3A. Associates ...
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Rabbit Polyclonal Anti-HDAC1 Antibody cited in 11 publications. Validated: WB, Simple Western, IHC, IHC-Fr, IHC-P. Tested ... Blogs on HDAC1. Check out the latest blog posts on HDAC1.. The role of DNMT3B in the co-incidence of methyltransferase and ... Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 ... Bioinformatics Tool for HDAC1 Antibody (NB100-56340). Discover related pathways, diseases and genes to HDAC1 Antibody (NB100- ...
The antibody reacts with HDAC1 of human, rat and mouse origin. Application Anti-Histone Deacetylase 1 (HDAC1) antibody can be ... Anti-Histone Deacetylase 1 (HDAC1) antibody specifically recognizes histone deacetylase 1and reacts specifically with HDAC1 of ... Anti-Histone Deacetylase 1 (HDAC1) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution * MDL ... HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. ...
Fast delivery of HDAC1 knockout Human Cell Lines for the study of gene function. Created by CRISPR/Cas9 genome editing. ... Human HDAC1 knockout cell line 7bp deletion. Genotype: HDAC1 HAP1 knockout HGNC Symbol: HDAC1 Price: $5,950.00 ... Human HDAC1 knockout cell line 8bp deletion. Genotype: HDAC1 HAP1 knockout HGNC Symbol: HDAC1 Price: $5,950.00 ... HDAC1 HAP1 knockout Cell Culture. Biosafety Level. 1 Media Type. IMDM, 10% FCS Freeze Medium. IMDM, 20% FCS, 10% DMSO Revival. ...
HDAC1 Protein Protein Interaction Antibody Pair, Abnova 1 Set, Unlabeled Life Sciences:Antibodies:Primary Antibodies:Protein ... Antibody pair set content: 1. RUNX1T1 rabbit purified polyclonal antibody (20 ug) 2. HDAC1 mouse monoclonal antibody (40 ug) * ... Includes: RUNX1T1 rabbit purified polyclonal antibody (20µg); HDAC1 mouse monoclonal antibody (40µg); Reagents are sufficient ... and the other against the HDAC1 protein for use in in situ Proximity Ligation Assay. ...
Inhibition of HDAC1 activity by p25/Cdk5 was identified as an underlying mechanism for these events, and HDAC1 gain of function ... HDAC1 siRNA or control (random sequence) siRNA transfected neurons are indicated by arrows. The HDAC1 siRNA transfected neurons ... Loss of HDAC1 or pharmacological inhibition of HDAC1 results in DNA damage, cell cycle reentry, and neurotoxicity ... Primary cortical were transfected with p25-GFP plus blank vector, flag-HDAC1, or catalytic dead flag-HDAC1-H141A mutant. At 24h ...
410 verschiedene HDAC1 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar! ... hdac-1, HDAC1, hdac1-b, Hdac1-ps, hdac1b, HISTONE DEACETYLASE, histone deacetylase 1, HISTONE DEACETYLASE 19, HISTONE ... Hdac1 is required for expression of erm (zeige ETV5 Antikörper) and fgf20a in rhombomeres; Hdac1-dependent expression of these ... in vivo role of HDAC1 in regulating cell cycle progression is region-specific, as HDAC1 promotes cell cycle exit in the retina ...
Phospho-HDAC1 (Ser421, Ser423) Polyclonal Antibody from Invitrogen for Western Blot, Immunofluorescence, Immunocytochemistry ... Cite Phospho-HDAC1 (Ser421, Ser423) Polyclonal Antibody. The following antibody was used in this experiment: Phospho-HDAC1 ( ... Western blot analysis of Phospho-HDAC1 (Ser421, Ser423) using Anti-Phospho-HDAC1 (Ser421, Ser423) Polyclonal Antibody (Product ... Immunohistochemical analysis of Phospho-HDAC1 pSer421+pSer423 in paraffin-embedded human liver carcinoma using Phospho-HDAC1 ...
Finally, DNMT1 knock down also decreases HDAC1 binding to the RGS10 promoter in chemoresistant cells, suggesting HDAC1 ... Inhibition of HDAC1 and DNMT1 modulate RGS10 expression and decrease ovarian cancer chemoresistance PLoS One. 2014 Jan 27;9(1): ... Knockdown of HDAC1 or DNMT1 expression, and pharmacological inhibition of DNMT or HDAC enzymatic activity, significantly ... Our results suggest that HDAC1 and DNMT1 contribute to the suppression of RGS10 during acquired chemoresistance and support ...
Associations between HDAC1 mRNA expression and different clinicopathological factors were sought. It was found that HDAC1 mRNA ... Quantitation of HDAC1 mRNA expression in invasive carcinoma of the breast* Breast Cancer Res Treat. 2005 Nov;94(1):11-6. doi: ... The aim of this study was to determine the expression of the HDAC1 gene in malignant human breast tissue and to correlate our ... Patients with tumors displaying high levels of HDAC1 mRNA expression tended to have a better prognosis in terms of both disease ...
Histone deacetylase 1 (HDAC1) was found to bind to the Fas promoter in T cells, and butyrate inhibits HDAC1 activity to induce ... Right: ChIP analysis of HDAC1, HDAC2, and acetyl-H3K9 association with the mouse Fas promoter. The HDAC1-, HDAC2-, and acetyl- ... D: inhibition of HDAC1 activity by butyrate. Recombinant human HDAC1 was assayed for its activity using a commercially ... C: HDAC1 association with the Fas promoter and butyrate-induced Fas promoter hyperacetylation. Left: mouse Fas promoter ...
HDAC1 (histone deacetylase 1) is a 65 kD member of the Class I histone deacetylase family, which is similar to the yeast RPD3 ... This product has been discontinued, please use alternate product: Clone 10E2/HDAC1 and Poly6074.. Please contact Sales ...
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Orally active histone deacetylase (HDAC) inhibitor (IC50 = 0.9, 0.9, 1.2, and >20 µM for HDAC1, HDAC2, HDAC3 and HDAC8 ...
Invitrogen Anti-HDAC1 Polyclonal, Catalog # PA1-860-A647. Tested in Immunofluorescence (IF) applications. This antibody reacts ... Cite HDAC1 Polyclonal Antibody, Alexa Fluor 647. The following antibody was used in this experiment: HDAC1 Polyclonal Antibody ... HDAC1 is a class I histone deacetylase containing 482 amino acid residues. HDAC1 has been shown to interact directly with ... Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1 ...
The HDAC1 polyclonal antibody ( Cat # PAB2325 ) is used in Western blot to detect HDAC1 in ZR-75-1 cell lysate.. *PAB2325 ... HDAC1 monoclonal antibody (M02), clone 3E1. Western Blot analysis of HDAC1 expression in NIH/3T3 ( Cat # L018V1 ).. *H00003065- ... HDAC1 monoclonal antibody (M02), clone 3E1 Western Blot analysis of HDAC1 expression in Hela NE ( Cat # L013V3 ).. *H00003065- ... HDAC1 monoclonal antibody (M06), clone 1D6. Western Blot analysis of HDAC1 expression in NIH/3T3 ( Cat # L018V1 ).. *H00003065- ...
PrimePCR™ Probe Assay: HDAC1, Rhesus Monkey Histone deacetylase Assay Type: Probe Assay Design: Exonic Application: Gene ... PrimePCR™ Probe Assay: HDAC1, Rhesus Monkey Histone deacetylase 1 Assay Type: Probe Assay Design: Exonic Application: Gene ... PrimePCR™ PreAmp for SYBR® Green Assay: HDAC1, Rhesus Monkey Reaction: 400 reactions Gene-specific PCR primers for the unbiased ... PrimePCR™ PreAmp for SYBR® Green Assay: HDAC1, Rhesus Monkey Reaction: 400 reactions Gene-specific PCR primers for the unbiased ...
HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and ... Equal HDAC1 loading was confirmed by probing membranes with anti-HDAC1 antibody, clone Poly6074 (Cat. No. 607401) at a 1:1000 ... Equal HDAC1 loading was confirmed by probing membranes with anti-HDAC1 antibody, clone Poly6074 (Cat. No. 607401) at a 1:1000 ... This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.. This clone recognizes zebrafish HDAC1 ...
... and Hdac1−/− embryonic stem cells. We also use microarray analysis of retrotransposon expression to show that the pluripotency- ... and identifies the histone deacetylase Hdac1 as a regulator of retrotransposons in this cell type. These computational ...
This review will discuss evidence supporting the involvement of HDAC1 and HDAC3 in polyglutamine disorders, including ... Huntingtons disease, and the use of HDAC1- and HDAC3-selective HDAC inhibitors as therapeutic intervention for these disorders ... HDAC1. HDAC1 is perhaps the most widely studied of all the HDAC enzymes. HDAC1 exhibits a high degree of homology to HDAC2 (85 ... HDAC1 expression has been measured in human brain samples, with inconsistent results [20,21,62]. HDAC1 mRNA was found to be ...
To knock down rat HDAC1 and HDAC2,MISSION siRNAs from Sigma were used. The sequences of siRNAs were as follows: for HDAC1 ... Re-ChIP assay revealed that Sp3, REST, HDAC1, and HDAC2 (Fig. 6e,g,h, lanes 4), and Sp1, HIF-1, and p300 (Fig. 6f,i, lanes 5) ... Primary ChIP products for anti-Sp3 (I Ab) were subjected to re-ChIP with anti-REST, anti-HDAC1, and anti-HDAC2 (II Ab) (e, g, h ... NCX3 is not modulated by the Sp3/REST/HDAC1/2 complex in tMCAO and by Sp1/HIF-1/p300 in PC+tMCAO. a, b, qRT-PCR and ...
AntibodyHistone DeacetylasesHDACHDACsInhibitorsChromatinMonoclonalPromoterRpd3InhibitorImmunogenZebrafishOverexpressionMaintaining HDAC1 activityAssociated with transcriptional repressionProteinsTranscriptional repressionGenesInhibition of HDAC1HDAC2 and HDAC3KnockoutSelective HDAC1RepressionTumorsInhibitsHumanTumorImmunohistochemistryLysateDifferentiationCloneAntibodiesDeacetylase activityApoptosisVivoNeuronsRecruitsPharmacologicalTranscriptionComplexes
- Western Blot: HDAC1 Antibody [NB100-- Analysis of HDAC1 in HEK293 cell lysate with this antibody. (novusbio.com)
- Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-- Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). (novusbio.com)
- Simple Western: HDAC1 Antibody [NB100-- Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. (novusbio.com)
- This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 1-5, 433-448 and 467-482 of human HDAC1 (Genbank Accession no. (novusbio.com)
- Anti-Histone Deacetylase 1 (HDAC1) antibody can be used in chromatin immunoprecipitation. (sigmaaldrich.com)
- Anti-Histone Deacetylase 1 (HDAC1) antibody specifically recognizes histone deacetylase 1and reacts specifically with HDAC1 of human, rat and mouse. (sigmaaldrich.com)
- The antibody is also useful for the detection of HDAC1 by immunohistochemistry. (sigmaaldrich.com)
- The antibody reacts with HDAC1 of human, rat and mouse origin. (sigmaaldrich.com)
- This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the RUNX1T1 protein, and the other against the HDAC1 protein for use in in situ Proximity Ligation Assay. (fishersci.com)
- HDAC1 mouse monoclonal antibody (40 ug) *Reagents are sufficient for at least 30-50 assays using recommended protocols. (fishersci.com)
- The following antibody was used in this experiment: Phospho-HDAC1 (Ser421, Ser423) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-36911, RRID AB_2553810. (thermofisher.com)
- The following antibody was used in this experiment: HDAC1 Polyclonal Antibody, Alexa Fluor 647 from Thermo Fisher Scientific, catalog # PA1-860-A647, RRID AB_2662018. (thermofisher.com)
- Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human testis. (acris-antibodies.com)
- Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. (acris-antibodies.com)
- HDAC1 monoclonal antibody (M02), clone 3E1. (acris-antibodies.com)
- Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody. (acris-antibodies.com)
- Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6.Lane 1: HDAC1 transfected lysate(55.1 KDa).Lane 2: Non-transfected lysate. (acris-antibodies.com)
- HDAC1 monoclonal antibody (M06), clone 1D6. (acris-antibodies.com)
- Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole for 16 hours were resolved on a 4-20% Tris-glycine gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-HDAC1 Phospho (Ser406) Antibody, clone BT-15, for 2 hours at room temperature. (biolegend.com)
- Equal HDAC1 loading was confirmed by probing membranes with anti-HDAC1 antibody, clone Poly6074 (Cat. (biolegend.com)
- Untreated HELA cells (left) and HELA cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with Purified anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) followed by PE Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) and DAPI. (biolegend.com)
- When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. (biolegend.com)
- Mouse monoclonal antibody raised against a full length recombinant HDAC1. (abnova.com)
- HDAC1 monoclonal antibody - Classic to my shopping cart. (diagenode.com)
- Request a quote for a bulk order for HDAC1 monoclonal antibody - Classic . (diagenode.com)
- Monoclonal antibody raised in mouse against human HDAC1 (Histone deacetylase 1), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal region of the protein. (diagenode.com)
- ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against HDAC1 (Cat. (diagenode.com)
- Nuclear extracts from HeLa cells (40 μg) were analysed by Western blot using the Diagenode monoclonal antibody against HDAC1 (Cat. (diagenode.com)
- Whole cell extracts (40 μg) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Diagenode antibody against HDAC1 (Cat. (diagenode.com)
- HeLa cells were stained with the Diagenode antibody against HDAC1 (Cat. (diagenode.com)
- The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. (diagenode.com)
- HDAC1 Antibody - ChIP-seq Grade to my shopping cart. (diagenode.com)
- Request a quote for a bulk order for HDAC1 Antibody - ChIP-seq Grade . (diagenode.com)
- ChIP was performed with the Diagenode antibody against HDAC1 (Cat. (diagenode.com)
- ChIP was performed on sheared chromatin from 4,000,000 HeLa cells using 2 μg of the Diagenode antibody against HDAC1 (Cat. (diagenode.com)
- To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against HDAC1 (Cat. (diagenode.com)
- The specificity of the Diagenode antibody against HDAC1 (Cat. (diagenode.com)
- This figure clearly shows the high specificity of the antibody for HDAC1. (diagenode.com)
- Ab2-hdac1 from the ZFIN antibody database . (zfin.org)
- Western blot analysis of extracts of various cell lines, using HDAC1 antibody (A0238) at 1:1000 dilution. (abclonal.com)
- Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (A0238) at 1:1000 dilution. (abclonal.com)
- Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (A0238) at dilution of 1:100 (40x lens). (abclonal.com)
- Immunofluorescence analysis of A549 cells using HDAC1 antibody (A0238). (abclonal.com)
- Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (A0238). (abclonal.com)
- Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilition of 1:1000. (abclonal.com)
- Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. (thermofisher.com)
- Here we found that Sp1 is a transcriptional activator, whereas Sp3 is a transcriptional repressor of ncx1, and that both bind ncx1-Br in a sequence-specific manner, modulating ncx1 transcription through the Sp1 sites C-E. Furthermore, by transient middle cerebral artery occlusion (tMCAO) in rats, the transcriptional repressors Sp3 and REST colocalized with the two histone-deacetylases (HDACs) HDAC1 and HDAC2 on the ncx1-Br, with a consequent hypoacetylation. (jneurosci.org)
- Entinostat (also known as MS-275 or SNDX-275), a derivative of 2-aminophenyl benzamides, is a potent and orally-available inhibitor of histone deacetylases (HDACs), a family of enzymes associated with a variety of well-characterized cellular oncogenes and tumor suppressors, that potently inhibits class I HDACs, including HDAC1, HDAC3 and HDAC8, with values of 50% inhibition concentration IC 50 of 0.368 μM, 0.501 μM and 63.4 μM respectively. (apexbt.com)
- We show here that histone deacetylases 1 and 2 (HDAC1/2) are essential for the specification of neural crest cells into Schwann cell precursors and satellite glia, which express the early determinants of their lineage myelin protein zero (P0) and/or fatty acid binding protein 7 (Fabp7). (elsevier.com)
- The KDAC family consists of 11 members, namely histone deacetylases HDAC1 to HDAC11, but it is not known which KDAC members play a role in cytokine-mediated beta cell death. (springer.com)
- Part of the core histone deacetylase (HDAC) complex composed of HDAC1 , HDAC2 , RBBP4 and RBBP7 . (rcsb.org)
- Knockdown of HDAC1 or DNMT1 expression, and pharmacological inhibition of DNMT or HDAC enzymatic activity, significantly increases RGS10 expression and cisplatin-mediated cell death. (nih.gov)
- Orally active histone deacetylase (HDAC) inhibitor (IC 50 = 0.9, 0.9, 1.2, and >20 µM for HDAC1, HDAC2, HDAC3 and HDAC8 respectively) (ref1). (activemotif.com)
- This review will discuss evidence supporting the involvement of HDAC1 and HDAC3 in polyglutamine disorders, including Huntington's disease, and the use of HDAC1- and HDAC3-selective HDAC inhibitors as therapeutic intervention for these disorders. (mdpi.com)
- Provided herein are compounds, pharmaceutical compositions comprising such compounds, and methods of using such compounds to treat or prevent diseases or disorders associated with HDAC activity, particularly diseases or disorders that involve activity of HDAC1 and/or HDAC2. (patents.com)
- We also demonstrate that clinically available HDAC inhibitors (HDACi) targeting HDAC1 and HDAC7 can be used to preferentially target CSCs. (pubmedcentralcanada.ca)
- Histone deacetylase (HDAC) inhibitor (ID50 values are 0.25 and 0.3 μM for HDAC1 and HDAC3 respectively). (apexbt.com)
- HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes. (sigmaaldrich.com)
- Utilizing a novel CSC model, we discovered that the HDACs, HDAC1 and HDAC7, are specifically over-expressed in CSCs when compared to non-stem-tumour-cells (nsTCs). (pubmedcentralcanada.ca)
- Class I HDACs, which include HDAC1 and HDAC2, are expressed in the CNS, although their specific role in neuronal function has not been studied. (jneurosci.org)
- To delineate the contribution of HDAC1 and HDAC2 in the brain, we have used pharmacological inhibitors of HDACs and mice with conditional alleles to HDAC1 and HDAC2. (jneurosci.org)
- with HDAC1 and HDAC2 expressed in the hippocampus ( MacDonald and Roskams, 2008 ), although the role of individual HDACs in the CNS is largely unknown. (jneurosci.org)
- With respect to carcinogenesis, class 1 HDACs (HDAC1,2,3 and 8) seem to be the most important ones. (beds.ac.uk)
- Selective HDAC1/HDAC2 inhibitors induce neuroblastoma differentiation. (cdc.gov)
- In addition, understanding the structural and functional differences between HDAC1 and HDAC2 might shed light on new strategies for the development of isoform specific inhibitors for HDAC1 or 2 that can be used in the treatment of leukemia and other cancer. (grantome.com)
- These results imply that HDAC1 is an important component of the CBFb-SMMHC transcriptional complex, and that leukemia cells expressing the fusion protein may be sensitive to treatment with HDAC1 inhibitors. (elsevier.com)
- Gfi1 knockdown in MC4 cells blocked multiple myeloma-induced recruitment of HDAC1 and EZH2 to Runx2 , acquisition of repressive chromatin architecture, and suppression of osteoblast differentiation. (aacrjournals.org)
- Importantly, inhibition of EZH2 or HDAC1 activity in pre-osteoblasts after multiple myeloma exposure in vitro or in osteoblast precursors from patients with multiple myeloma reversed the repressive chromatin architecture at Runx2 and rescued osteoblast differentiation. (aacrjournals.org)
- A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells. (everestbiotech.com)
- These results show that methylation of Sp1 causes it to act as a negative regulator by recruiting Suv39H1 and HDAC1 to induce chromatin remodeling. (elsevier.com)
- Transcripts of the chromatin remodeler chd4a /Mi-2, the histone deacetylase hdac1 /HDAC1/2, the retinoblastoma-binding protein rbb4 /RBBP4/7, and the metastasis-associated antigen mta2 /MTA were specifically co-induced in the blastema during adult and embryonic fin regeneration, and these transcripts displayed a similar spatial and temporal expression patterns. (biomedcentral.com)
- this interaction affects HDAC1 activity on MYOG promoter and thus inhibits MYOD1 transcriptional activity. (rcsb.org)
- Finally, DNMT1 knock down also decreases HDAC1 binding to the RGS10 promoter in chemoresistant cells, suggesting HDAC1 recruitment to RGS10 promoters requires DNMT1 activity. (nih.gov)
- Histone deacetylase 1 (HDAC1) was found to bind to the Fas promoter in T cells, and butyrate inhibits HDAC1 activity to induce Fas promoter hyperacetylation and Fas upregulation in T cells. (nih.gov)
- HDAC1 and c-Myc were decreased and RNA polymerase II increased in proteins bound to the p21WAF1 promoter. (aacrjournals.org)
- In neural crest cells, HDAC1/2 induced expression of the transcription factor Pax3 by binding and activating the Pax3 promoter. (elsevier.com)
- In addition, HDAC1/2 were bound to the P0 promoter and activated P0 transcription. (elsevier.com)
- A distinction between HDAC1 and 3 was also apparent with respect to crh promoter occupancy. (unthsc.edu)
- Investigation of the mechanism by which methylation decreased Sp1 activity found that methylation of Sp1 increased the recruitment of Su(var) 3-9 homologue 1(Suv39H1) and histone deacetylase 1 (HDAC1) to the cyclin B1 promoter, resulting in deacetylation and methylation of histone H3 and subsequent downregulation of cyclin B1. (elsevier.com)
- Our data suggests overexpression of HDAC1 leads to p15/INK4b suppression, a cell cycle inhibitor, potentially explaining the mechanism behind these observed effects. (byu.edu)
- CI994 is a selective inhibitor of HDAC1 with IC50 value of 0.57 μM . (apexbt.com)
- Intriguingly, the neuroprotective effects of hUC-MSCs with HDAC1 silence on behavioral performance of TBI mice was markedly attenuated by LY294002, an inhibitor of the PI3K/AKT pathway. (elsevier.com)
- Using a knock-in mouse model expressing CBFb- SMMHC, we found that in vivo treatment with the HDAC1 inhibitor entinostat decreased leukemic burden, and induced differentiation and apoptosis of leukemia cells. (elsevier.com)
- Eradication of metastatic melanoma through cooperative expression of RNA-based HDAC1 inhibitor and p73 by oncolytic adenovirus. (iegt-rostock.de)
- This clone recognizes zebrafish HDAC1 phosphorylated at Ser406 2 . (biolegend.com)
- Cunliffe ( 2004 ) observed that in zebrafish mutations missing hdac1 map, encephalon constructions were smaller than in its wild type siblings. (yogurtlandsweettreats.com)
- Further working in this research lab reveals that in the same zebrafish hdac1 mutations, the degrees of extra proneural cistrons such as ascl1a and neurod4 show a dramatic lessening from 12 hours post fertilisation onwards as compared with wild type embryos. (yogurtlandsweettreats.com)
- Taken together, these surveies suggest that Hdac1 regulates proneural cistron look during cardinal nervous system development in zebrafish from the earliest phases of neurogenesis onwards. (yogurtlandsweettreats.com)
- The lessening look of proneural cistrons asl1a, asl1b and neurod4 might be the ground of the decrease in neurogenesis in zebrafish hdac1 mutations. (yogurtlandsweettreats.com)
- Here we demonstrate that HDAC1 overexpression is sufficient to induce β-cell proliferation, enhance β-cell survival upon exposure to apoptotic stimuli and maintains glucose stimulated insulin secretion (GSIS). (byu.edu)
- These data demonstrate that HDAC1 overexpression is sufficient to induce β-cell proliferation and enhance cell survival while maintaining glucose stimulated insulin secretion. (byu.edu)
- HDAC1 overexpression led to a significant increase in proliferation and a shift towards the undifferentiated cytokeratin (CK) profile in a PC3M derivative clone constitutively expressing HDAC1. (ox.ac.uk)
- HDAC1 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4. (thermofisher.com)
- Moreover, it suggests that the association of NF-κB with the HDAC1 and HDAC2 corepressor proteins functions to repress expression of NF-κB-regulated genes as well as to control the induced level of expression of these genes. (asm.org)
- HDAC1 is also required for active transcription of certain proteins. (wikibooks.org)
- Inhibition of HDAC1 activity by p25/Cdk5 was identified as an underlying mechanism for these events, and HDAC1 gain of function provided potent protection against DNA damage and neurotoxicity in cultured neurons and an in vivo model for ischemia. (nih.gov)
- Our results suggest that HDAC1 and DNMT1 contribute to the suppression of RGS10 during acquired chemoresistance and support inhibition of HDAC1 and DNMT1 as an adjuvant therapeutic approach to overcome ovarian cancer chemoresistance. (nih.gov)
- In addition, chemical inhibition of Hdac1 and morpholino-mediated knockdown of chd4a , mta2 , and rbb4 impaired regenerative outgrowth, resulting in reduction in blastema cell proliferation and in differentiation defects. (biomedcentral.com)
- 3). Further investigation demonstrated that selective HDAC1/HDAC2 inhibition using compounds or RNA interference induced differentiation and decreased viability in neuroblastoma cell lines. (cdc.gov)
- Therefore, by applying a chemical genomic screening approach, we identified selective HDAC1/HDAC2 inhibition as a strategy to induce neuroblastoma differentiation. (cdc.gov)
- It was found that HDAC1 mRNA was expressed at significantly higher levels in tumors from patients over 50 years of age and in those tumors without axillary lymph node involvement, that are less than 2 cm, that are of a non-high histological grade, that are HER2 negative and that are ERalpha/PgR positive. (nih.gov)
- Patients with tumors displaying high levels of HDAC1 mRNA expression tended to have a better prognosis in terms of both disease-free and overall survival. (nih.gov)
- In human tumors, we identified somatic mutations catalytically inactivating HDAC1. (uva.nl)
- synthetic peptide corresponding to amino acids 466-482 of human and mouse HDAC1. (sigmaaldrich.com)
- PA1860A647 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells. (thermofisher.com)
- ab94165 is a 293T cell transfected lysate in which Human HDAC1 has been transiently over-expressed using a pCMV-HDAC1 plasmid. (abcam.cn)
- Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2). (abclonal.com)
- Synthesized peptide derived from the Internal region of human HDAC1. (biotecnika.org)
- METHODS: By immunohistochemistry, HDAC1 protein expression was investigated in human prostate specimens and the CWR22 mouse xenograft model. (ox.ac.uk)
- Sequential ChIP assays demonstrated that ERα represses p53-mediated transcriptional activation in human breast cancer cells by recruiting nuclear receptor corepressors (NCoR and SMRT) and histone deacetylase 1 (HDAC1). (pnas.org)
- HDAC1 , - 2 , - 6 and - 11 were found to be expressed and regulated by cytokines in human islets. (springer.com)
- However, little is known about the effects of HDAC1 on the survival and efficacy of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in vivo. (elsevier.com)
- Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. (biolegend.com)
- Therefore, our data provide support for the potential important roles of HDAC1 in cell proliferation and HDAC2 in migration and differentiation. (mysciencework.com)
- However, GATA-1 remains associated with HDAC1 containing corepressor complexes during differentiation of erythroid cells. (grantome.com)
- BACKGROUND: Histone deacetylase 1 (HDAC1) is a co-repressor involved in differentiation and proliferation control. (ox.ac.uk)
- Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. (biolegend.com)
- We previously found that HDAC1 can be acetylated and that acetylated HDAC1 completely lost deacetylase activity (Qiu et al, 2006). (grantome.com)
- Butyrate suppresses colonic inflammation through HDAC1-dependent Fas upregulation and Fas-mediated apoptosis of T cells. (nih.gov)
- In vivo dual inactivation of Hdac1 and Hdac2 in hematopoietic cells resulted in apoptosis of megakaryocytes with concomitant anemia and thrombocytopenia, suggesting that similar toxicities observed in patients treated with HDACi are caused by on-target inhibition. (uva.nl)
- In this study, our results showed that HDAC1 silence promoted hUC-MSCs engraftment in the hippocampus and increased the neuroprotective effects of hUC-MSCs in TBI mouse model, which was accompanied by improved neurological function, enhanced neurogenesis, decreased neural apoptosis, and reduced oxidative stress in the hippocampus. (elsevier.com)
- Consistently, in vivo genetic deletion of HDAC1/2 in mouse neural crest cells led to strongly decreased Sox10 expression, no detectable Pax3, virtually no satellite glia, and no Schwann cell precursors in dorsal root ganglia and peripheral nerves. (elsevier.com)
- In vivo studies revealed that CRH-immunoreactive (-ir) neurons contained HDAC1- and HDAC3-ir. (unthsc.edu)
- Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. (abcam.com)
- Deletion of both HDAC1 and HDAC2 in floxed mouse neurons during early synaptic development resulted in a similar facilitation of excitatory synapse maturation and a modest increase in synapse numbers. (jneurosci.org)