A species of HAEMOPHILUS that appears to be the pathogen or causative agent of the sexually transmitted disease, CHANCROID.
Acute, localized autoinoculable infectious disease usually acquired through sexual contact. Caused by HAEMOPHILUS DUCREYI, it occurs endemically almost worldwide, especially in tropical and subtropical countries and more commonly in seaports and urban areas than in rural areas.
A genus of PASTEURELLACEAE that consists of several species occurring in animals and humans. Its organisms are described as gram-negative, facultatively anaerobic, coccobacillus or rod-shaped, and nonmotile.
A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII.
Infections with bacteria of the genus HAEMOPHILUS.
A lesion on the surface of the skin or a mucous surface, produced by the sloughing of inflammatory necrotic tissue.
Vaccines or candidate vaccines containing antigenic polysaccharides from Haemophilus influenzae and designed to prevent infection. The vaccine can contain the polysaccharides alone or more frequently polysaccharides conjugated to carrier molecules. It is also seen as a combined vaccine with diphtheria-tetanus-pertussis vaccine.
Infections of the nervous system caused by bacteria of the genus HAEMOPHILUS, and marked by prominent inflammation of the MENINGES. HAEMOPHILUS INFLUENZAE TYPE B is the most common causative organism. The condition primarily affects children under 6 years of age but may occur in adults.
Proteins isolated from the outer membrane of Gram-negative bacteria.
A species of gram-negative bacteria (currently incertae sedis) causing multisystem disease in CATTLE.
Pathological processes involving the male reproductive tract (GENITALIA, MALE).
A type of H. influenzae isolated most frequently from biotype I. Prior to vaccine availability, it was a leading cause of childhood meningitis.
A species of gram-negative bacteria in the genus HAEMOPHILUS found, in the normal upper respiratory tract of SWINE.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Proteins found in any species of bacterium.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic.
An enzyme that forms CMP-acylneuraminic acids, which donate the N-acylneuraminic acid residues to the terminal sugar residue of a ganglioside or glycoprotein. EC 2.7.7.43.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
The use of humans as investigational subjects.
A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Bacteriocins elaborated by mutant strains of Pseudomonas aeruginosa. They are protein or protein-lipopolysaccharide complexes lethal to other strains of the same or related species.
The functional hereditary units of BACTERIA.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST.

Experimental infection of human volunteers with Haemophilus ducreyi does not confer protection against subsequent challenge. (1/298)

Two groups of human volunteers were inoculated with 2 doses of live Haemophilus ducreyi 35000HP. The reinfection group consisted of 7 subjects who previously had participated in experimental infection with 35000HP to the pustular stage of disease. The control group consisted of 7 naive subjects. Papules developed at 92.8% (95% confidence interval [CI], 66.1%-99.8%) of sites inoculated with live bacteria, in the reinfection group, and at 85.7% (95% CI, 57.2%-98. 2%) of sites in the control group. Sixty-nine percent (95% CI, 36. 8%-90.9%) of papules evolved into pustules in the reinfection group, compared with 41% (95% CI, 15.2%-72.3%) in the control group. The recovery rates of H. ducreyi from surface cultures and the histopathology of biopsies obtained from both groups were similar. Thus, experimental infection to the pustular stage of disease does not provide protective immunity against subsequent challenge.  (+info)

Binding of Haemophilus ducreyi to extracellular matrix proteins. (2/298)

We developed an enzyme-linked immunosorbent assay-based assay to assess Haemophilus ducreyi binding to extracellular matrix (ECM) proteins. H. ducreyi 35000HP bound to fibronectin, laminin, and type I and III collagen but not to type IV, V, or VI collagen or elastin. Isogenic strains with mutations in ftpA or losB bound as well as the parent, suggesting that neither pili nor full-length lipooligosaccharide is required for H. ducreyi to bind to ECM proteins.  (+info)

Molecular characterization of Haemophilus ducreyi strains from Jackson, Mississippi, and New Orleans, Louisiana. (3/298)

Chancroid, a sexually transmitted disease caused by Haemophilus ducreyi, is one of the most common genital ulcer diseases in developing countries. In the United States, while less common, the disease has been associated with outbreaks in inner cities, particularly among persons who engage in sex for drugs or money. Two outbreaks of chancroid were recently studied in the United States, one in New Orleans (from 1990 to 1992) and one in Jackson, Mississippi (from 1994 to 1995). By use of ribotyping, plasmid content, and antibiotic susceptibility, the chancroid cases in New Orleans were found to be due to a limited number of strains, consistent with a limited introduction of H. ducreyi into this community. The H. ducreyi isolates from New Orleans and Jackson had different ribotype patterns, suggesting that the two outbreaks were probably not linked.  (+info)

Prevalence of, antibody response to, and immunity induced by Haemophilus ducreyi hemolysin. (4/298)

Haemophilus ducreyi, the etiologic agent of chancroid, a genital ulcer disease, produces a cell-associated hemolysin whose role in virulence is not well defined. Hemolysin is encoded by two genes, hhdA and hhdB, which, based on their homology to Serratia marcescens shlA and shlB genes, are believed to encode the hemolysin structural protein and a protein required for secretion and modification of this protein, respectively. In this study, we determined the prevalence and expression of the hemolysin genes in 90 H. ducreyi isolates obtained from diverse geographic locations from 1952 to 1996 and found that all strains contained DNA homologous to the hhdB and hhdA genes. In addition, all strains expressed a hemolytic activity. We also determined that hemolysin is expressed in vivo and is immunogenic, as indicated by the induction of antibodies to hemolysin in both the primate and rabbit disease models as well as in human patients with naturally acquired chancroid. Wild-type strain 35000 and isogenic hemolysin-negative mutants showed no difference in lesion development in the temperature-dependent rabbit model. However, immunization of rabbits with the purified hemolysin protein reduced the recovery of wild-type H. ducreyi, but not hemolysin-negative mutants, from lesions. Our study indicates that hemolysin is a possible candidate for vaccine development due to its immunogenicity, expression in vitro and in vivo by most, if not all, strains, and the effect of immunization on reducing the recovery of viable H. ducreyi in experimental disease in rabbits.  (+info)

Target cell range of Haemophilus ducreyi hemolysin and its involvement in invasion of human epithelial cells. (5/298)

Haemophilus ducreyi, the causative agent of chancroid, produces a hemolysin, whose role in virulence is not well defined. To assess the possible role of hemolysin in pathogenesis, we evaluated its target cell range by using wild-type H. ducreyi 35000, nonhemolytic mutants with the hemolysin structural gene deleted, and isogenic strains expressing different amounts of hemolytic activity. The cytotoxicity of the various cell types was assessed by quantitating the release of lactate dehydrogenase into culture supernatants as a measure of cell lysis. In these experiments, human foreskin fibroblasts, human foreskin epithelial cells, and, to a lesser extent, HEp-2 cells were lysed by H. ducreyi hemolysin. Hemolysin also lysed human blood mononuclear cells and immune system cell lines including U937 macrophage-like cells, T lymphocytes, and B lymphocytes. In contrast, human polymorphonuclear leukocytes were not sensitive to hemolysin under the conditions tested. We also analyzed the effect of hemolysin on invasion of human epithelial cells and found that H. ducreyi strains expressing cloned hemolysin genes showed a 10-fold increase in invasion compared to the control strain. These data support the hypothesis that the H. ducreyi hemolysin is important in the pathogenesis of chancroid and may contribute to ulcer formation, invasion of epithelial cells, and evasion of the immune response.  (+info)

Characterization of a Haemophilus ducreyi mutant deficient in expression of cytolethal distending toxin. (6/298)

Haemophilus ducreyi expresses a soluble cytolethal distending toxin (CDT) that kills HeLa, HEp-2, and other human epithelial cells in vitro. H. ducreyi CDT activity is encoded by a three-gene cluster (cdtABC), and antibody to the cdtC gene product can neutralize CDT activity in vitro (L. D. Cope, S. R. Lumbley, J. L. Latimer, J. Klesney-Tait, M. K. Stevens, L. S. Johnson, M. Purven, R. S. Munson, Jr., T. Lagergard, J. D. Radolf, and E. J. Hansen, Proc. Natl. Acad. Sci. USA 94:4056-4061, 1997). Culture supernatant fluid from a recombinant Escherichia coli strain containing the H. ducreyi cdtABC gene cluster readily killed both HeLa cells and HaCaT keratinocytes and had a modest inhibitory effect on the growth of human foreskin fibroblasts. Insertional inactivation of the cdtC gene in this recombinant E. coli strain eliminated the ability of this strain to kill HeLa cells and HaCaT keratinocytes. This mutated H. ducreyi cdtABC gene cluster was used to construct an isogenic H. ducreyi cdtC mutant. Monoclonal antibodies against the H. ducreyi CdtA, CdtB, and CdtC proteins were used to characterize protein expression by this cdtC mutant. Culture supernatant fluid from this H. ducreyi cdtC mutant did not detectably affect any of the human cells used in this study. The presence of the wild-type H. ducreyi cdtC gene in trans in this H. ducreyi mutant restored its ability to express a CDT that killed both HeLa cells and HaCaT keratinocytes. The isogenic H. ducreyi cdtC mutant was shown to be as virulent as its wild-type parent strain in the temperature-dependent rabbit model for experimental chancroid. Lack of expression of the H. ducreyi CdtC protein also did not affect the ability of this H. ducreyi mutant to survive in the skin of rabbits.  (+info)

Genital ulcers: etiology, clinical diagnosis, and associated human immunodeficiency virus infection in Kingston, Jamaica. (7/298)

Individuals presenting consecutively with genital ulcers in Kingston, Jamaica, underwent serological testing for human immunodeficiency virus (HIV) infection, chlamydial infection, and syphilis. Ulcer material was analyzed by multiplex polymerase chain reaction (M-PCR) analysis. DNA from herpes simplex virus (HSV), Haemophilus ducreyi, and Treponema pallidum was detected in 158 (52.0%), 72 (23.7%), and 31 (10.2%) of 304 ulcer specimens. Of the 304 subjects, 67 (22%) were HIV-seropositive and 64 (21%) were T. pallidum-seroreactive. Granuloma inguinale was clinically diagnosed in nine (13.4%) of 67 ulcers negative by M-PCR analysis and in 12 (5.1%) of 237 ulcers positive by M-PCR analysis (P = .03). Lymphogranuloma venereum was clinically diagnosed in eight patients. Compared with M-PCR analysis, the sensitivity and specificity of a clinical diagnosis of syphilis, herpes, and chancroid were 67.7%, 53.8%, and 75% and 91.2%, 83.6%, and 75.4%, respectively. Reactive syphilis serology was 74% sensitive and 85% specific compared with M-PCR analysis. Reported contact with a prostitute in the preceding 3 months was associated with chancroid (P = .009), reactive syphilis serology (P = .011), and HIV infection (P = .007). The relatively poor accuracy of clinical and locally available laboratory diagnoses pleads for syndromic management of genital ulcers in Jamaica. Prevention efforts should be intensified.  (+info)

Immune cells are required for cutaneous ulceration in a swine model of chancroid. (8/298)

Cutaneous lesions of the human sexually transmitted genital ulcer disease chancroid are characterized by the presence of intraepidermal pustules, keratinocyte cytopathology, and epidermal and dermal erosion. These lesions are replete with neutrophils, macrophages, and CD4(+) T cells and contain very low numbers of cells of Haemophilus ducreyi, the bacterial agent of chancroid. We examined lesion formation by H. ducreyi in a pig model by using cyclophosphamide (CPA)-induced immune cell deficiency to distinguish between host and bacterial contributions to chancroid ulcer formation. Histologic presentation of H. ducreyi-induced lesions in CPA-treated pigs differed from ulcers that developed in immune-competent animals in that pustules did not form and surface epithelia remained intact. However, these lesions had significant suprabasal keratinocyte cytotoxicity. These results demonstrate that the host immune response was required for chancroid ulceration, while bacterial products were at least partially responsible for the keratinocyte cytopathology associated with chancroid lesions in the pig. The low numbers of H. ducreyi present in lesions in humans and immune-competent pigs have prevented localization of these organisms within skin. However, H. ducreyi organisms were readily visualized in lesion biopsies from infected CPA-treated pigs by immunoelectron microscopy. These bacteria were extracellular and associated with necrotic host cells in the epidermis and dermis. The relative abundance of H. ducreyi in inoculated CPA-treated pig skin suggests control of bacterial replication by host immune cells during natural human infection.  (+info)

Haemophilus ducreyi previously has been shown to inhibit the phagocytosis of both secondary targets and itself by certain cells in vitro. Wild-type H. ducreyi strain 35000HP contains two genes, lspA1 and lspA2, whose encoded protein products are predicted to be 456 and 543 kDa, respectively. An isogenic mutant of H. ducreyi 35000HP with inactivated lspA1 and lspA2 genes has been shown to exhibit substantially decreased virulence in the temperature-dependent rabbit model for chancroid. This lspA1 lspA2 mutant was tested for its ability to inhibit phagocytosis of immunoglobulin G-opsonized particles by differentiated HL-60 and U-937 cells and by J774A.1 cells. The wild-type strain H. ducreyi 35000HP readily inhibited phagocytosis, whereas the lspA1 lspA2 mutant was unable to inhibit phagocytosis. Similarly, the wild-type strain was resistant to phagocytosis, whereas the lspA1 lspA2 mutant was readily engulfed by phagocytes. This inhibitory effect of wild-type H. ducreyi on phagocytic activity was ...
TY - JOUR. T1 - Experimental infection of human volunteers with Haemophilus ducreyi. T2 - Fifteen years of clinical data and experience. AU - Janowicz, Diane M.. AU - Ofner, Susan. AU - Katz, Barry P.. AU - Spinola, Stanley M.. PY - 2009/6/1. Y1 - 2009/6/1. N2 - Haemophilus ducreyi causes chancroid, which facilitates transmission of human immunodeficiency virus type 1. To better understand the biology of H. ducreyi, we developed a human inoculation model. In the present article, we describe clinical outcomes for 267 volunteers who were infected with H. ducreyi. There was a relationship between papule formation and estimated delivered dose. The outcome (either pustule formation or resolution) of infected sites for a given subject was not independent; the most important determinants of pustule formation were sex and host effects. When 41 subjects were infected a second time, their outcomes segregated toward their initial outcome, confirming the host effect. Subjects with pustules developed local ...
TY - JOUR. T1 - Haemophilus ducreyi targets Src family protein tyrosine kinases to inhibit phagocytic signaling. AU - Mock, Jason R.. AU - Vakevainen, Merja. AU - Deng, Kaiping. AU - Latimer, Jo L.. AU - Young, Jennifer A.. AU - Van Oers, Nicolai S C. AU - Greenberg, Steven. AU - Hansen, Eric J.. PY - 2005/12. Y1 - 2005/12. N2 - Haemophilus ducreyi, the etiologic agent of the sexually transmitted disease chancroid, has been shown to inhibit phagocytosis of both itself and secondary targets in vitro. Immunodepletion of LspA proteins from H. ducreyi culture supernatant fluid abolished this inhibitory effect, indicating that the LspA proteins are necessary for the inhibition of phagocytosis by H. ducreyi. Fluorescence microscopy revealed that macrophages incubated with wild-type H. ducreyi, but not with a lspA1 lspA2 mutant, were unable to complete development of the phagocytic cup around immunoglobulin G-opsonized targets. Examination of the phosphotyrosine protein profiles of these two sets of ...
TY - JOUR. T1 - Activation of CpxRA in Haemophilus ducreyi primarily inhibits the expression of Its targets, including major virulence determinants. AU - Gangaiah, Dharanesh. AU - Zhang, Xinjun. AU - Fortney, Kate R.. AU - Baker, Beth. AU - Liu, Yunlong. AU - Munson, Robert S.. AU - Spinola, Stanley. PY - 2013/8. Y1 - 2013/8. N2 - Haemophilus ducreyi causes chancroid, a genital ulcer disease that facilitates the transmission of human immunodeficiency virus type 1. In humans, H. ducreyi is surrounded by phagocytes and must adapt to a hostile environment to survive. To sense and respond to environmental cues, bacteria frequently use two-component signal transduction (2CST) systems. The only obvious 2CST system in H. ducreyi is CpxRA; CpxR is a response regulator, and CpxA is a sensor kinase. Previous studies by Hansen and coworkers showed that CpxR directly represses the expression of dsrA, the lspB-lspA2 operon, and the flp operon, which are required for virulence in humans. They further showed ...
1. Ballard RC, Duncan MO, Fehler HG, Dangor Y, Exposto FL, Latif AS. Treating chancroid: summary of studies in southern Africa. Genitourin Med 1989;65:54-7. [PubMed]. 2. Bauer ME, Townsend CA, Ronald AR, Spinola SM. Localization of Haemophilus ducreyi in naturally acquired chancroidal ulcers. Microbes Infect 2006;8(9-10):2465-2468. [PubMed]. 3. Behets FM, Liomba G, Lule G, Dallabetta G, Hoffman IF, Hamilton HA, Moeng S, Cohen MS. Sexually transmitted diseases and human immunodeficiency virus control in Malawi: a field study of genital ulcer disease. J Infect Dis 1995;171:451-5.[PubMed]. 4. Bogaerts J, Kestens L, Martinez Tello W, Akingeneye J, Mukantabana V, Verhaegen J, Van Dyck E, Piot P. Failure of treatment for chancroid in Rwanda is not related to human immunodeficiency virus infection: in vitro resistance to Haemophilus ducreyi to trimethoprim-sulphamethoxazole. Clin Infect Dis 1995;20:924-30. [PubMed]. 5 Centers for Disease Control and Prevention. Sexually transmitted disease ...
Haemophilus ducreyi is a fastidious gram-negative coccobacillus bacteria, which causes the sexually transmitted disease chancroid, a major cause of genital ulceration in developing countries characterized by painful sores on the genitalia. Chancroid starts as an erythematous papular lesion that breaks down into a painful bleeding ulcer with a necrotic base and ragged edge. H. ducreyi can be cultured on chocolate agar. It is best treated with a macrolide like azithromycin and a third-generation cephalosporin like ceftriaxone. H. ducreyi gram stain appears as school of fish. H. ducreyi is an opportunistic microorganism that infects its host by way of breaks in the skin or epidermis. Inflammation then takes place as the area of infection is inundated with lymphocytes, macrophages, and granulocytes. This pyrogenic inflammation causes regional lymphadenitis in the sexually transmitted bacillus chancroid. Although antigen detection, serology, and genetic amplification methods are sometimes used to ...
TY - JOUR. T1 - Identification of tandem genes involved in lipooligosaccharide expression by Haemophilus ducreyi. AU - Stevens, Marla K.. AU - Klesney-Tait, Julia. AU - Lumbley, Sheryl. AU - Walters, K. A.. AU - Joffe, A. Mark. AU - Radolf, Justin D.. AU - Hansen, Eric J.. PY - 1997/2/1. Y1 - 1997/2/1. N2 - A transposon insertion mutant of Haemophilus ducreyi 35000 possessing a truncated lipooligosaccharide (LOS) failed to hind the LOS-specific monoclonal antibody 3E6 (M. K. Stevens, L. D. Cope, J. D. Radolf and E. J. Hansen, Infect. Immun. 63:2976-2982, 1995). This transposon was found to have inserted into the first of two tandem genes and also caused a deletion of chromosomal DNA upstream of this gene. These two genes, designated lbgA and lbgB, encoded predicted proteins with molecular masses of 25,788 and 40,236 Da which showed homology with proteins which function in lipopolysaccharide biosynthetic in other gram-negative bacteria. The tandem arrangement of the lbgA and lbgB genes was found ...
A monoclonal antibody raised against Haemophilus ducreyi was tested for its sensitivity and specificity as an immunofluorescence (IF) reagent using simulated vaginal smears containing H. ducreyi, smears taken from skin lesions of mice infected with H. ducreyi and patients from South Africa, Thailand and Malaysia with clinically diagnosed chancroid. The IF test was more sensitive than culture or Gram staining in the simulated smears, theoretically detecting less than 4 organisms/sample. It detected H. ducreyi in 95% of the animal lesions compared with 14% detected by culture. Immunofluorescence testing identified over 90% of culture-positive cases of chancroid but also detected organisms in some culture-negative cases where clinical evidence for the diagnosis was strong. These results suggest that this antibody may provide a simple, rapid and sensitive means of detecting H. ducreyi in cases of chancroid.
TY - JOUR. T1 - Standardization of an enzyme immunoassay for human antibody to Haemophilus ducreyi. AU - Desjardins, M. AU - Thompson, CE. AU - Filion, LG. AU - Ndinya-Achola, JO. AU - Plummer, FA. AU - Ronald, AR. AU - Piot, P. AU - Cameron, DW. PY - 1992. Y1 - 1992. KW - B780-tropical-medicine. KW - Bacteriology. KW - Haemophilus ducreyi. KW - Antibody detection. KW - Immunoenzyme techniques. KW - Immunoglobulin G. KW - Immunoglobulin M. KW - Screening. KW - STD. KW - Sexually transmitted diseases. KW - Laboratory techniques and procedures. M3 - A1: Web of Science-article. VL - 30. SP - 2019. EP - 2024. JO - Journal of Clinical Microbiology. JF - Journal of Clinical Microbiology. SN - 0095-1137. ER - ...
Haemophilus ducreyi ATCC ® 700724D-5™ Designation: Genomic DNA from Haemophilus ducreyi 35000HP TypeStrain=False Application:
† Ulcerative genital disease in the United States is commonly due to herpes simplex or syphilis. Until recently, chancroid, an infection caused by the gram-nega
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
To investigate if temperature affects the interaction of Haemophilus ducreyi with human epithelial cells, nine strains were used to evaluate the adhesion kinetics of the organism at 33°C and 37°C. The effect of the free toxin on the epithelial cells at those temperatures was also assessed. The cyto-adherence kinetics of H. ducreyi to the epithelial cells was significantly greater at 33°C (10 times more) than at 37°C in all seven clinical isolates tested. There was a significant difference in cell-associated H. ducreyi at 33°C as compared with 37°C. Control strains showed similar adhesion properties at both temperatures. However, the virulent strain CIP542 adhered in larger amounts than the avirulent strain A77. Electron microscopy revealed that there was more tissue necrosis at the lower than the higher temperature. The effect of the free toxin was the same at each temperature. However, strain A77 had significantly lower toxicity than strain CIP542 and the clinical isolates. These results suggest
Pyocin lysis has previously been used as a strategy to identify LOS mutants of H. ducreyi and N. gonorrhoeae(8, 14). Although the actual mechanism of lysis is poorly understood, a recent report by Lee et al. suggests that these particles contain nucleic acid (27). In this study, we used pyocin to select for LOS mutants of H. ducreyi strain 35000. One such mutant, HD35000R, produced a LOS molecule that lacked the MAb 3F11 epitope and migrated with an increased mobility on SDS-PAGE. Complementation of this mutant with a plasmid library containingH. ducreyi 35000 chromosomal DNA resulted in the identification of a clone expressing wild-type LOS. Western blot analysis confirmed that this transformant, HD35000R(pLS88.8), expressed the LOS epitope reactive with MAb 3F11. The sequence analysis of the complementing plasmid revealed a 3.2-kb DNA insert containing four complete ORFs. The putative protein product of ORF1 shared similarity (37%) with the E. coli WaaQ, the heptosyltransferase responsible for ...
Summary Routine procedures used to isolate Haemophilus ducreyi in a busy laboratory are reported. Identification was based on colony morphology and nutritional and biochemical properties of 120 fresh isolates of H. ducreyi. These isolates grew very well on Gonococcal Agar and Mueller-Hinton Agar incubated at 34°C in candle extinction jars containing moistened filter paper. Colonies varied in size, giving a polymorphic appearance. They were smooth, dome-shaped, and buff-yellow to grey in colour, and measured 2 mm in diameter. They could be pushed intact across the agar surface. By microscopic examination of gram-stained smears the isolates were gram-negative coccobacilli arranged in short chains, clumps or whorls and occasionally in typical
Sigma-Aldrich offers abstracts and full-text articles by [Dana A Dodd, Randall G Worth, Michael K Rosen, Sergio Grinstein, Nicolai S C van Oers, Eric J Hansen].
SOP: Cloning of PCR Product. Add 1 ml room-temperature LB broth (without ampicillin) to the tube. A-9518) agar plate pre-spread with 20 µl X-Gal (50 mg/ml.haemophilus ducreyi. FAQ. Haemophilus ducreyi; Haemophilus;. and then to plate it on blood agar and chocolate agar.thoroughly and pour plates. YEPD (YPD) PLATES: Agar 20 g. Peptone 10 g. Yeast Extract 900 ml distilled water: 5 ml of 1 M HCl (do not mouth pipette) 20 g. Agar Autoclave.1 Genomic tools and cDNA derived markers for butterflies. the library was plated out on LB agar plates with Xgal blue/white 116 screening and 0.1% ampicillin,.. ...
Thank you for sharing this Journal of Bacteriology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
Comprehensive instructions for specimen collection, special requirements, specimen handling, testing methods and turnaround times.
|p|Azithral is used for infections caused by susceptible organisms in-|/p||p|Upper respiratory tract infections including sinusitis, pharyngitis and tonsillitis|/p||p|Lower respiratory tract infections including bronchitis, acute bacterial exacerbations of chronic obstructive pulmonary|/p||p|disease (COPD)|/p||p|Otitis media|/p||p|Skin and soft tissue infections including cellulitis, pyoderma, erysipelas, wound infections|/p||p|Diarrhea, Shigellosis|/p||p|Sexually transmitted diseases, especially in the treatment of non-gonococcal urethritis and cervicitis due to Chlamydia trachomatis|/p||p|Genital ulcer disease in men due to Haemophilus ducreyi (chancroid)|/p||p|Mild or moderate typhoid due to multiple-antibacterial resistant organisms|/p||p|Prophylaxis against a-hemolytic (viridans group) streptococcal bacterial endocarditis|/p||p|Other infections including odontogenic infections, bartonella infections, toxoplasmosis, babesiosis|/p|
Abdullah, M.T., Nepliounev, I., Alfonina, G., Ram, S., Rice, P., Elkins, C. (2005). Killing of dsrA mutants of haemophilus ducreyi by normal human serum occurs via the classical complement pathway and is initiated by immunoglobulin m binding. Infection and Immunity. 73: 3431-3439 ...
bacteria HupA protein: HupA - Hemoglobin Utilization Protein A; a hemoglobin-binding protein; MW 108 kDa; GenBank U34048 (Haemophilus ducreyi)
Week 1 started fairly slowly, which gave me a chance to read up on several papers about research already carried on PcaK and the AAHS family. I began by culturing E. Coli which had been transformed with a plasmid containing the PcaK gene of the extremophile Haemophilus ducreyi, and then induced these via addition of arabinose (to turn on T7 RNA polymerase expression) and IPTG (to turn on protein expression). Unfortunately, the cells started to die on induction of protein expression - a possible explanation for this being that the protein is toxic to E. coli growth. This may lead to a low final concentration once purified, as its possible that the cells that survived were those that were not expressing the protein! I proceeded to harvest the cells via centrifugation and then resuspend the pellets in PBS buffer and TCEP (reduces disulphide bonds). Next I got to use a cell disrupter to lyse the cells and, after an inital centrifugation to remove debris and unlysed cells, I pelleted the membranes ...
Hemophilus Ducreyi Symptom Checker: Possible causes include Sexually Transmitted Disease. Check the full list of possible causes and conditions now! Talk to our Chatbot to narrow down your search.
Chancroid is a sexually transmitted disease (STD) caused by a bacterium called Haemophilus Ducreyi. The disease is completely curable but causes ulcers, generally in the genital area.. Chancroid is mainly passed from person to person with genital contact, but can also be spread via oral intercourse, anal intercourse, and through areas of broken skin. In addition, the infection can be transmitted non-sexually when fluid from Chancroid ulcers are transferred from an infected person to another individual.. The disease is more common in men that in women, and as a result, Chancroid sores (usually referred to as soft chancres) often develop in the groin area with painful and swollen lymph glands.. History of Chancroid. It is understood that Chancroid had its origins from Africa, and it is quite a common disease in the Caribbean and Southwest Asia. However, the disease is becoming more and more prevalent in the United States.. Symptoms of Chancroid. If you develop symptoms of Chancroid, they will most ...
Chancroid is a sexually transmitted disease caused by Haemophilus ducreyi infection leading to genital ulcers and painful lymphadenopathy, from the online textbook of urology by D. Manski
U08MM1072. Gonorrhoea, Chlamydia, Genital herpes are common sexually transmitted diseases that people are aware of, but are you among many to be coming across Chancroid for the first time?. Chancroid is a bacterial infection that can be spread through sexual contact. this bacteria is called Haemophilus Ducreyi and it is most common in Africa and southwest Asia. Chancroid can occur in both men and women and in 1 day to 2 weeks after becoming infected, a small bump appears on the genitals which becomes an ulcer within a day after it appears. For men, only 1 ulcer may appear in the foreskin, shaft of the penis, head of the penis or the scrotum. For women, 4 or more ulcers may appear in the outer lips of the vagina (Labia Majora), inner vagina lips and inner thighs.. Chancroid can cause urethral fistulas (a scar that develops in the urethra) and scars on the foreskin of the penis. People with Chancroid should be checked for other STDs like Syphillis, HIV or Genital Herpes. For people with HIV, ...
Dear Reader,. It seems youre ready to sharpen your chancroid (starts with a sh sound) knowledge! On to it then: Chancroid is a bacterial infection transmitted through contact with sores on an infected individuals skin. The bacteria, Haemophilus ducreyi, can lead to painful, open sores and swollen lymph nodes, usually in the groin area. Its relatively rare in the United States (including in infants and children), and appears to be more prevalent in some regions of Africa and the Caribbean. Its also more common in those assigned male at birth that those assigned female at birth (but those assigned female can be asymptomatic carriers). A proper diagnosis is key to appropriate treatment, as symptoms of chancroid can be confused with primary syphilis. But, fear not, because its treatable; antibiotics are typically used to speed up the healing process, but the sores may also clear up on their own. If left untreated though, chancroid can lead to skin damage. Keep reading for even more detailed ...
Tetracyclines and sulfonamides are no longer effective for the treatment of chancroid in many parts of the world. Rifampin and trimethoprim both possess in vitro activity against Haemophilus ducreyi, the causative agent of chancroid. In a randomized, controlled study, 22 patients with H. ducreyi-positive genital ulcers received 600 mg of rifampin once daily for three days, and 32 patients received 600 mg of rifampin plus 160 mg of trimethoprim once daily for three days. Both regimens rapidly eradicated H. ducreyi from ulcers, with subsequent healing of ulcers and buboes. Two relapses of ulcers and one therapeutic failure were observed in the rifampin-trimethoprim group, whereas no relapses or failures were noted in the rifampin group. In addition, all of 16 H. ducreyi-negative ulcers responded rapidly to treatment with either regimen. In an uncontrolled, open study, 22 H. ducreyi-positive ulcers were treated with a single dose of rifampin (600 mg) plus trimethoprim (160 mg). Ulcers and buboes ...
These Pathogen Safety Data Sheets, regulated under Workplace Hazardous Materials Information System (WHMIS) legislation, are produced for personnel working in the life sciences as quick safety reference material relating to infectious micro-organisms.
Chancroid is a type of sexually transmitted disease. Chancroid is a bacterial (Hemophilus ducreyi) infection with an incubation period of 3-14 days. The incubation period is the time between exposure to the infection (sexual event) and the development of symptoms associated with this infection. Common presentation includes one or more painful lumps, or swellings, to the groin area (usually on both sides). Females may also have a vaginal discharge and lower pelvic pain that is more severe during intercourse. Males and females may have multiple ragged ulcers to the penis or vulva.. Treatment is with orally administered antibiotics. Abscess formation (pockets of pus under the skin) is not uncommon, and incision and drainage of the abscess (by a physician) will be necessary in these cases. Regular application of warm wet compresses to the swollen inguinal area, in addition to treatment with antibiotics, can help eradicate this infection. Sexual intercourse should be avoided until the infection as ...
Chancroid is a sexually transmitted infection caused by the bacteria Haemophilus ducreyi. It causes painful open sores in the genital area and may cause swollen lymph nodes in the groin. It is rare in North America and Europe and is sometimes difficult to diagnose. Find out about treatment and prevention.
en] Anti-Bacterial Agents/therapeutic use ; Chancre/complications/pathology ; Chancroid/complications/pathology ; Erythromycin/therapeutic use ; Haemophilus Infections/complications/diagnosis/drug therapy/pathology ; Haemophilus ducreyi/isolation & purification ; Humans ; Male ; Syphilis/complications/diagnosis/drug therapy/pathology ; Treatment Outcome ; Treponema pallidum/isolation & purification ; Young ...
Treatment for Chancroid. Find Doctors Near You, Book Appointment, Consult Online, View Doctor Fees, Address, Phone Numbers and Reviews. Doctors for Chancroid | Lybrate
The cytolethal distending toxins (CDTs) are a family of exotoxins produced by a wide range of Gram-negative bacteria. They are known for causing genotoxic stress to the cell, resulting in growth arrest and eventually apoptotic cell death. Nevertheless, there is evidence that CDTs can also perturb the innate immune responses, by regulating inflammatory cytokine production and molecular mediators of bone remodeling in various cell types. These cellular and molecular events may in turn have an effect in enhancing local inflammation in diseases where CDT-producing bacteria are involved, such as Aggregatibacter actinomycetemcomitans, Haemophilus ducreyi, Campylobacter jejuni and Helicobacter hepaticus. One special example is the induction of pathological bone destruction in periodontitis. The opportunistic oral pathogen Aggregatibatcer actinoycemetemcomitans, which is involved in the aggressive form of the disease, can regulate the molecular mechanisms of bone remodeling in a manner that favors bone
2015 Strategies in the Chancroid Diagnostics Testing Market The report presents a detailed analysis of the Chancroid diagnostics market in the US, Europe (France, Germany, - Market research report and industry analysis - 9079292
[220 Pages Report] Check for Discount on Chancroid: US, Europe, Japan--Market Analysis, Competitive Intelligence, Technology Trends, Opportunities for Suppliers report by Venture Planning Group. The report presents a detailed analysis of the Chancroid diagnostics...
Public Health Labs. In addition to test volume and sales projections, the report presents sales and market share estimates for major suppliers of Chancroid tests.. Also, the report examines the market applications of DNA Probes, Monoclonal Antibodies, Immunoassays, IT and other technologies; reviews features and operating characteristics of automated analyzers; profiles leading suppliers and recent market entrants developing innovative technologies and products; and identifies emerging business expansion opportunities, alternative market penetration strategies, market entry barriers and risks, and strategic planning issues and concerns.. Contains 220 pages and 8 tables. ...
Chancroid is a bacterial infection that causes open sores on the genitals. We explain how its spread, the symptoms to expect, and how its treated.
Chancroid is a type of sexually transmitted bacterial infection causing painful sores on the genitals. It is a rare type of infection affecting men and women
Compare risks and benefits of common medications used for Chancroid. Find the most popular drugs, view ratings, user reviews, and more...
OK horn dogs (yea this includes myself) heres the facts. STD Fact Sheets Information on 11 common sexually transmitted diseases CHANCROID WHAT IT IS: A...
All of our articles are screened by our News Screening Department to the best of their ability to be sure that all of our articles are true and accurate ...
All of our articles are screened by our News Screening Department to the best of their ability to be sure that all of our articles are true and accurate ...
Learn about the causes, symptoms, diagnosis & treatment of Sexually Transmitted Diseases (STDs) from the Home Version of the Merck Manuals.
The STD Project Established in April of 2012 during STD Awareness Month, The STD Project is a multi-award-winning independent website and progressive movement eradicating STD stigma by facilitating and encouraging awareness, education, and acceptance through story-telling and resource recommendations. We are taking steps toward modern-day sexual health and prevention by advocating for conscientious and informed decisions.. ...
Acne pustules and blackheads occur when oil blocks a hair follicle and forms a plug. Blackheads are open at the surface of the skin, causing the dirt and oil in the pore to oxidize and turn dark. Pustules are closed, hidden beneath the skin. They appear a
NAA, serology, microscopy and culture, cytology, (Genital swab).. See also Human papillomavirus infection, Vaginal discharge, Trichomonas vaginalis infection, Toxic shock syndrome, Candidiasis, Syphilis, Pelvic inflammatory disease, Orchitis, Mycoplasma infection, Lymphogranuloma venereum, Human papillomavirus infection, Herpes simplex virus infection, HIV infection, Chancroid (Haemophilus ducreyi infection), Granuloma inguinale (donovanosis), Gonococcal infection, Gonorrhoea, Bartholins abcess.. ...
The goal was to estimate the cofactor effect of genital ulcer disease (GUD) on the risk of HIV transmission during a single heterosexual exposure. The relation between the risk ratio observed in an epidemiological study and the per-exposure cofactor effect was investigated. Given simple assumptions, …
Ceftriaxone in a dose of 250 mg given intramuscularly is currently recommended for the treatment of chancroid. Among 133 men in Nairobi, Kenya, with culture-proven chancroid, who were treated with ceftriaxone, treatment ...
Australian STI Management Guidelines for Use in Primary Care are nationally endorsed guidelines on the testing, diagnosis, management and treatment of STIs.
The physician poked a pustule on the shin with a needle and there was a small amount of purulent drainage. I want to use 10060 and another coder says
SEXUALLY TRANSMITTED INFECTIONS Laminated 20 x 26, Describes and illustrates the following STIs: genital warts, genital herpes, chancroid, syphi
Male : Indurated testes. Nocturnal emissions. Impotency. Onanism (in sycotic children). Handling (Stra.). Chancroids, metastasing into warts. Gleets. Enlarged prostate.
A. L. Hansen Mfg. Co. is a respected and trusted manufacturer of commercial, vehicle and industrial hardware. Our products have long been recognized as the industry standard for quality and dependability. Established in 1920 A. L. Hansen has grown to be a full service manufacturer.. ...
Svalard Parka by Helly Hansen at Zappos.com. Read Helly Hansen Svalard Parka product reviews, or select the size, width, and color of your choice.
Den globale bioscience virksomhed udvider og styrker forskning- & udviklingsfaciliteterne i Hørsholm for at imødekomme den stigende efterspørgsel efter naturlige og bæredygtige produkter.. Chr. Hansen har indgået samarbejde med PensionDanmark om en markant udvidelse af sit hovedkontor og forsknings- og udviklingsfaciliteter i Hørsholm nord for København, mens Catella Corporate Finance A/S har været finansiel rådgiver. Projektet omfatter modernisering og etablering af et innovation campus med laboratorier til yderligere 250 forskere. Formålet er at skabe fremtidssikrede faciliteter til forskning og udvikling og sikre Chr. Hansens stærke position som foretrukken leverandør af naturlige ingredienser og mikrobielle løsninger til den globale fødevareindustri, kosttilskudsbranchen og landbrugssektoren.. Ud over laboratorier opføres også et nyt, stort applikationscenter, hvor Chr. Hansen i samarbejde med sine kunder fra hele verden kan afprøve og skræddersy nye, innovative produkter i ...
Dear Valued Coworkers and Clients, (Rev-1.1) Date 4-1-2020 CJ Hansen Co., Inc. is committed to the safety and well-being of our community. This enduring
The Rin Chair from Fritz Hansen was designed by Hiromichi Konno in 2008. Rin has a nest-shaped shell made from moulded plastic, sitting atop a 4 star polished or lacquered aluminium base. The Fritz Hansen Rin Chair is a multi-functional tub chair designed to sit in perfect harmony with both Japanese and Danish design traditions.
The best pimples to pop are small whiteheads, blackheads, and pustules (looks like a whitehead, but the skin around is red and inflamed). Whiteheads appear when dead skin cells, oil, and bacteria are trapped in your pores. Blackheads are similar in that they are caused by a clogged pore, but the fundamental difference is that with whiteheads, the pore remains closed as compared to blackheads in which the pore is open. Pustules are small bumps on the skin that contain pus or fluid. The safest type to extract at home are the ones which have collected a small amount of pus or keratin in the middle and are very close to the surface of skin, and those that are not very red or deep: these are usually simple blackheads, whiteheads, or pustules, says Dr. Nazarian. Its also important to note that you should never touch deep cysts. While it may be very tempting to pop deep cysts, these should ideally be reserved to professionals as they can be difficult to treat due to the depth of the active lesion, ...
Browse Hannafords Sally Hansen to begin building your online shopping list or grocery cart. Browse 1000s of fresh, local, quality products.
Helly Hansen Jacke, Hose und mehr +++ bis zu 40% sparen ✔100 Tage Rückgaberecht ✚ Kauf auf Rechnung llll➤ VERSAND HEUTE bei Bestellung Mo-Fr bis 16h!
Helly Hansen Jacke, Hose und mehr +++ bis zu 40% sparen ✔100 Tage Rückgaberecht ✚ Kauf auf Rechnung llll➤ VERSAND HEUTE bei Bestellung Mo-Fr bis 16h!
Fans have begged Deadliest Catch star Sig Hansen to stop smoking, after this weeks episode showed him suffering chest pains a year after his heart attack.
A pustule on the skin often heals after it drains. However, a pustule in your coronary artery results in a different outcome. When it releases its contents into the bloodstream, a blood clot forms around it and often leads to a heart attack. Reducing inflammation is a key component in the strategy of preventing heart attacks. ...
pock definition: A pustule brought on by smallpox or a similar eruptive disease.; A mark or scar remaining within the epidermis by such a pustule; a pockmark.; To mark with pocks; pit.; A pus filled inflammation…
Olsen, J. and Cowell, G. M. and Konigshofer, E. and Danielsen, E. M. and Moller, J. and Laustsen, L. and Hansen, O. C. and Welinder, K. G. and Engberg, J. and Hunziker, W. and Spiess, Martin and Sjostrom, H. and Noren, O. ...
"Haemophilus ducreyi (Chancroid) - Infectious Disease and Antimicrobial Agents". Haemophilus ducreyi at the NCBI Taxonomy ... Haemophilus ducreyi is a fastidious gram-negative coccobacillus bacteria. It causes the sexually transmitted disease chancroid ... H. ducreyi gram stain resembles a "school of fish." H. ducreyi is an opportunistic microorganism that infects its host by way ... Sexually transmitted disease Lewis, DA; Mitjà, O (February 2016). "Haemophilus ducreyi: from sexually transmitted infection to ...
Cole, L. E.; Kawula, T. H.; Toffer, K. L.; Elkins, C. (2002). "The Haemophilus ducreyi Serum Resistance Antigen DsrA Confers ... White, C. D.; Leduc, I.; Olsen, B.; Jeter, C.; Harris, C.; Elkins, C. (2005). "Haemophilus ducreyi Outer Membrane Determinants ... Elkins, C.; Morrow Jr, K. J.; Olsen, B. (2000). "Serum Resistance in Haemophilus ducreyi Requires Outer Membrane Protein DsrA ... Leduc, I.; Olsen, B.; Elkins, C. (2008). "Localization of the Domains of the Haemophilus ducreyi Trimeric Autotransporter DsrA ...
Haemophilus ducreyi infections can cause skin conditions that mimic primary yaws. People infected with Haemophilus ducreyi ... It seems that a recently diverged strain of Haemophilus ducreyi has evolved from being a sexually transmitted infection to ... Lewis, David A.; Mitjà, Oriol (February 2016). "Haemophilus ducreyi: from sexually transmitted infection to skin ulcer pathogen ...
... the SiaPQM TRAP transporter from Haemophilus influenzae and the SatABCD ABC transporter from Haemophilus ducreyi. Neuraminic ... Post DM, Mungur R, Gibson BW, Munson RS Jr (2005). "Identification of a novel sialic acid transporter in Haemophilus ducreyi". ... "Sialic acid transport in Haemophilus influenzae is essential for lipopolysaccharide sialylation and serum resistance and is ...
Lewis, David A (2014). "Epidemiology, clinical features, diagnosis and treatment of Haemophilus ducreyi - a disappearing ...
Martin PR, Shea RJ, Mulks MH (February 2001). "Identification of a plasmid-encoded gene from Haemophilus ducreyi which confers ... was first isolated in Haemophilus ducreyi, it was found to exhibit significant homology to the mammalian PBEF gene. Rongvaux et ...
Many of these bacteria, including Shigella dysenteriae, Haemophilus ducreyi, and Escherichia coli, infect humans. Bacteria that ... Medically important CDT producers include: Haemophilus ducreyi (chancroids) Aggregatibacter actinomycetemcomitans ( ... This paper was followed by a 1999 publication in Infectious Immunity, which demonstrated that H. ducreyi CDT causes cell death ... HducCDT for Haemaphilus ducreyi CDT). CDT toxins are genotoxins capable of directly damaging DNA in target cells. They are the ...
Main focus was on such bacteria as Haemophilus influenzae, H. ducreyi, and Streptococcus sp. Bordetella pertussis 1. She was a ... "Antibodies to Haemophilus influenzae and their bactericidal activity" (Ph.D. in Microbiology). She was 1988-1989 a post-doc at ...
He is particularly known for his studies of the chancroid and the coccobacillus haemophilus ducreyi. He was the father of ...
Murphy TF (2020). "Haemophilus Species, Including H. influenzae and H. ducreyi (Chancroid)". Mandell, Douglas, and Bennett's ... "Pinkbook: Haemophilus influenzae (Hib) , CDC". www.cdc.gov. 2022-09-22. Retrieved 2022-10-13. "Symptoms of Haemophilus ... "Haemophilus influenzae". NCBI Taxonomy Browser. 727. Type strain of Haemophilus influenzae at BacDive - the Bacterial Diversity ... However, there is no effective vaccine for the other types of capsulated Haemophilus inflenzae or Haemophilus inflenzae ...
Haemophilus ducreyi, Mannheimia haemolytica and Haemophilus parasuis, was supported by 9 CSIs. Based on these results, it was ... Haemophilus influenzae and Haemophilus somnus, was supported by 13 CSIs. Clade II, encompassing Actinobacillus pleuropneumoniae ...
Haemophilus ducreyi, another medically important Gram-negative coccobacillus, is observed in sexually transmitted disease, ... Haemophilus influenzae, Gardnerella vaginalis, and Chlamydia trachomatis are coccobacilli. Aggregatibacter ...
The Eib immunoglobulin-binding proteins from Escherichia coli were third, followed by the DsrA proteins of Haemophilus ducreyi ...
Other homologues have been found in Burkholderia species, Escherichia coli, Haemophilus ducreyi, Mannheimia species, ... Haemophilus ducreyi and Kingella kingae). N-linked glycosylation is an important process, especially in eukaryotes where over ... Haemophilus influenzae has an additional HMW1C homologue HMW2C, which together with the adhesin HMW2 forms a similar substrate- ... in Haemophilus influenzae and identified as a novel type of glycosyltransferase in 2010. The Actinobacillus pleuropneumoniae N- ...
Skin ulcers can resemble those caused by leishmaniasis, yaws, squamous cell carcinoma, Haemophilus ducreyi infection, and ...
... area Inner thighs Chancroid is a bacterial infection caused by the fastidious Gram-negative streptobacillus Haemophilus ducreyi ... H. ducreyi enters skin through microabrasions incurred during sexual intercourse. The incubation period of H. ducreyi infection ... Chancroid, caused by H. ducreyi has infrequently been associated with cases of Genital Ulcer Disease in the US but has been ... From bubo pus or ulcer secretions, H. ducreyi can be identified using special culture media; however, there is a ...
... the infection caused by a combination of Treponema pallidum and Haemophilus ducreyi, and true syphilis. In 1877 he was ...
Chancroid (Haemophilus ducreyi) Chlamydia (Chlamydia trachomatis) Gonorrhea (Neisseria gonorrhoeae) Granuloma inguinale or ( ...
... and haemophilus ducreyi (culture on special media currently not widely available in most hospital systems) if the individual is ... While rates of H. ducreyi infection seem to be declining according to a 2017 report, it is possible this may be due to lack of ... testing since H. ducreyi requires a very specialized culture medium which is not commonly available. Less common causes include ...
... urethritis caused by Ureaplasma urealyticum Relapsing fever due to Borrelia recurrentis Chancroid caused by Haemophilus ducreyi ... while some Haemophilus spp., Mycoplasma hominis, and Pseudomonas aeruginosa have developed resistance to varying degrees. It is ... infections Respiratory tract infections caused by Haemophilus influenzae Respiratory tract and urinary tract infections caused ...
Streptococcus pyogenes Aerobic and facultative anaerobic Gram-negative microorganisms Haemophilus ducreyi Haemophilus ...
Mycoplasma hominis Mycoplasma pneumoniae Pseudomonas aeruginosa Haemophilus ducreyi Sulfatide acts as a glycolipid receptor ... Haemophilus influenzae, Bordetella pertussis, Mycoplasma pneumoniae, Moraxella catarrhalis, and Pseudomonas aeruginosa cause ... Enterotoxigenic Escherichia coli TOP10 strain Campylobacter jejuni Mycoplasma hyopneumoniae Haemophilus influenzae ...
Haemophilus ducreyi) Chlamydia (Chlamydia trachomatis) Gonorrhea (Neisseria gonorrhoeae) Granuloma inguinale or (Klebsiella ...
... vaginalis Haemophilus Haemophilus ducreyi Haemophilus influenzae Haemophilus parainfluenzae Haemophilus pertussis Haemophilus ...
Treponema pallidum Chancroid is a lesion typical of infection with the bacterium Haemophilus ducreyi Chancres are typically ...
Haemophilus MeSH B03.440.450.600.450.125 - Haemophilus ducreyi MeSH B03.440.450.600.450.330 - Haemophilus influenzae MeSH ... Haemophilus MeSH B03.660.250.550.290.125 - Haemophilus ducreyi MeSH B03.660.250.550.290.330 - Haemophilus influenzae MeSH ... Haemophilus paraphrophilus MeSH B03.440.450.600.450.700 - Haemophilus parasuis MeSH B03.440.450.600.450.750 - Haemophilus ... Haemophilus paraphrophilus MeSH B03.660.250.550.290.700 - Haemophilus parasuis MeSH B03.660.250.550.290.750 - Haemophilus ...
... and Haemophilus ducreyi which causes chancroid. Certain C. jejuni LPS serotypes (attributed to certain tetra- and ... and Haemophilus spp. LOS plays a central role in maintaining the integrity and functionality of the outer membrane of the Gram ... Haemophilus somnus, a pathogen of cattle, has also been shown to display LOS phase variation, a characteristic which may help ... Howard MD, Cox AD, Weiser JN, Schurig GG, Inzana TJ (2000). "Antigenic diversity of Haemophilus somnus lipooligosaccharide: ...
... ducreyi, the causative agent of chancroid. All members are either aerobic or facultatively anaerobic. This genus has been found ... Alternatively, Haemophilus is sometimes cultured using the "Staph streak" technique: both Staphylococcus and Haemophilus ... Members of the genus Haemophilus will not grow on blood agar plates, as all species require at least one of these blood factors ... While Haemophilus bacteria are typically small coccobacilli, they are categorized as pleomorphic bacteria because of the wide ...
Haemophilus ducreyi, Haemophilus parasuis, and Mannheimia haemolytica. Molecular signatures in the form of CSIs have also been ... Haemophilus influenzae, Haemophilus somnus, and Mannheimia succiniciproducens, while the other includes Actinobacillus minor, ... Haemophilus influenzae was the first organism to have its genome sequenced and has been studied intensively by genetic and ... The genus Haemophilus is a notorious human pathogen associated with bacteremia, pneumonia, meningitis and chancroid. Other ...
The 2014 Ju-Jitsu World Championship were the 12th edition of the Ju-Jitsu World Championships, and were held in Paris, France from November 28 to November 30, 2014. 28.11.2014 - Men's and Women's Fighting System, Men's and Women's Jiu-Jitsu (ne-waza), Men's Duo System - Classic 29.11.2014 - Men's and Women's Fighting System, Men's and Women's Jiu-Jitsu (ne-waza), Women's Duo System - Classic 30.11.2014 - Men's Jiu-Jitsu (ne-waza), Mixed Duo System - Classic, Team event Vincent MATCZAK (2014-09-30). "4TH INVITAION TO WORLD CHAMPIONSHIP 2014" (PDF). Retrieved 2019-11-28.[dead link] Online results Official results (PDF) Mixed team event results (PDF) (All articles with dead external links, Articles with dead external links from April 2022, Ju-Jitsu World Championships, 2014 in French sport ...
DNA Replication Proteins - Haemophilus ...
Haemophilus ducreyi) case definitions; uniform criteria used to define a disease for public health surveillance. ... Chancroid (Haemophilus ducreyi) , 1996 Case Definition. *Chancroid (Haemophilus ducreyi) , 1990 Case Definition ...
All 3 oils demonstrated excellent activity against H. ducreyi, with MICs of 0.05 to 0.52 mg/mL and MLCs of 0.1-0.5 mg ... Antibiotic-resistant strains of H. ducreyi were equally susceptible to these 3 essential oils relative to non-resistant strains ... ducreyi using the agar dilution method. We also determined the minimum lethal concentration for each oil by subculturing from ... ducreyi. We determine the minimum inhibitory concentration (MIC) of Cinnamomum verum (cinnamon), Eugenia caryophyllus (clove) ...
... primarily Haemophilus ducreyi. We developed a novel molecular test to simultaneously detect T. pallidum and H. ducreyi based on ... Haemophilus Ducreyi LAMP Loop-mediated Isothermal Amplification Multiplex Mediator Displacement Loop-Mediated Isothermal ... Amplification For Detection Of Treponema Pallidum And Haemophilus Ducreyi Neglected Tropical Diseases PCR Research Treponema ... We validated the T. pallidum and H. ducreyi LAMP (TPHD-LAMP) by testing 293 clinical samples from patients with yaws-like ...
... ducreyi, smears taken from skin lesions of mice infected with H. ducreyi and patients from South Africa, Thailand and Malaysia ... It detected H. ducreyi in 95% of the animal lesions compared with 14% detected by culture. Immunofluorescence testing ... These results suggest that this antibody may provide a simple, rapid and sensitive means of detecting H. ducreyi in cases of ... A monoclonal antibody raised against Haemophilus ducreyi was tested for its sensitivity and specificity as an ...
Haemophilus ducreyi Cutaneous Ulcer Strains Are Nearly Identical to Class I Genital Ulcer Strains. PLoS Negl Trop Dis. 2015. 9 ... Haemophilus ducreyi inhibits phagocytosis by U-937 cells, a human macrophage-like cell line. Infect Immun. 2001 Aug. 69 (8): ... Pathophysiological concept of Haemophilus ducreyi infection (chancroid). Int J STD AIDS. 1992 Sep-Oct. 3 (5):319-23. [QxMD ... Chancroid and Haemophilus ducreyi: an update. Clin Microbiol Rev. 1995 Jul. 8 (3):357-75. [QxMD MEDLINE Link]. ...
Haemophilus ducreyi Cutaneous Ulcer Strains Are Nearly Identical to Class I Genital Ulcer Strains. PLoS Negl Trop Dis. 2015. 9 ... Haemophilus ducreyi inhibits phagocytosis by U-937 cells, a human macrophage-like cell line. Infect Immun. 2001 Aug. 69 (8): ... Pathophysiological concept of Haemophilus ducreyi infection (chancroid). Int J STD AIDS. 1992 Sep-Oct. 3 (5):319-23. [QxMD ... Chancroid and Haemophilus ducreyi: an update. Clin Microbiol Rev. 1995 Jul. 8 (3):357-75. [QxMD MEDLINE Link]. ...
Haemophilus species including H. influenzae and H. ducreyi (chancroid). In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, ... Chancroid is caused by a bacterium called Haemophilus ducreyi.. The infection is found in many parts of the world, such as ...
Haemophilus ducreyi. Haemophilus influenzae. Klebsiella granulomatis. Klebsiella species. Neisseria gonorrhoeae. Shigella ... Respiratory tract infections caused by Haemophilus influenzae.. -. Respiratory tract infections caused by Klebsiella species.. ...
Categories: Haemophilus ducreyi Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, CopyrightRestricted ...
The disease is caused by infection with Haemophilus ducreyi. Laboratory criteria for diagnosis --Isolation of H. ducreyi from a ... Haemophilus influenzae (Invasive Disease). Clinical description Invasive disease caused by Haemophilus influenzae may produce ... Cases of bacterial meningitis caused by Haemophilus influenzae, Neisseria meningitidis, group A Streptococcus, and Listeria ... Haemophilus influenzae, invasive drug-resistant invasive disease disease Streptococcal toxic-shock syndrome Hansen disease ( ...
See: Haemophilus ducreyi Test Name Chancre See: Treponema pallidum. Test Name Chicken Pox ...
Haemophilus ducreyi requires the flp gene cluster for microcolony formation in vitro. ...
Categories: Haemophilus ducreyi Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, CopyrightRestricted ...
What is chancroid? Chancroid is cause by the bacteria Haemophilus ducreyi. This bacteria is transmitted during sexual ...
Lipooligosaccharide and Cytolethal distending toxin of Haemophilus ducreyi and antibody responses.. Supervisor: Teresa ... The cytolethal distending toxin of Haemophilus ducreyi - purification and biological activity. 2005. Surpervisor: Teresa ...
HgbA of Haemophilus ducreyi. 1.B.14.2.14. Heme/hemoglobin receptor of 660 aas and 22 C-terminal β-strands with an N-terminal " ...
The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid Rudi ... Part of a ribosomal ribonucleic acid (rRNA) cistron of Haemophilus ducreyi was enzymically amplified using conserved primers ... Hybridization experiments with at least 41 H. ducreyi strains and 13 or 14 non-H. ducreyi strains revealed that all eight ... Comparisons of 16S rRNA sequences confirm that H. ducreyi is a member of the Pasteurellaceae though not closely related to ...
A sexually transmitted infection caused by the fastidious, gram-negative coccobacillus Haemophilus ducreyi, which is most ... Epidemiology of Haemophilus ducreyi Infections. Emerg Infect Dis. 2016 Jan;22(1):1-8. https://www.doi.org/10.3201/ ...
These include human papilloma virus (HPV), herpes simplex virus (HSV), Treponema pallidum, Haemophilus ducreyi, and donovanosis ... The differential diagnosis of these ulcers includes HSV-2 and Hemophilus ducreyi. Diagnosis is best made using a dark-field ...
Haemophilus ducreyi as a cause of skin ulcers in children from a yaws-endemic area of Papua New Guinea.. Lancet Global Health: ... extensive ulcerative yaws and ulcers caused by Haemophilus ducreyi1.. Early nodular lesions are occasionally confused with ...
Chancroid is an infection caused by the Streptobacillus Haemophilus ducreyi and transmitted by sexual activity. This Gram- ... Most Haemophilus ducreyi strains are tolerant to tetracycline, amoxicillin and sulfamethoxazole/trimetoprim. ...
Studies on the bacteriology of haemophilus ducreyi with special reference to the diagnosis of cancroid. Kbh. 1951. 27C. Book. ...
  • Haemophilus ducreyi , a fastidious gram-negative bac- Methods terium, is the causative agent of chancroid, a genital ulcer disease (GUD). (cdc.gov)
  • We searched the National the painful nature of the lesions, patients usually seek im- Library of Medicine through PubMed for " H. ducreyi ," mediate treatment, and asymptomatic carriage is therefore "chancroid," "genital ulcer," OR "skin ulceration" AND uncommon ( 2 ). (cdc.gov)
  • Haemophilus ducreyi is the bacterium responsible for the genital ulcer disease chancroid, a cofactor for the transmission of HIV, and it is resistant to many antibiotics. (biomedcentral.com)
  • Haemophilus ducreyi , a Gram-negative coccobacillus, is a strict human pathogen responsible for the development of chancroid, which is a sexually transmitted infection (STI) that causes the formation of genital ulcers 4-10 days after acquisition of the bacteria and persists in some cases for 1-3 months. (biomedcentral.com)
  • The development of antibiotic resistance in H. ducreyi is of particular concern given the connection between HIV-1 and chancroid[ 5 ]. (biomedcentral.com)
  • With the increasing resistance of strains of H. ducreyi to antibiotics currently in use and the threat that chancroid contributes to the spread of HIV, it seems prudent to search for alternatives to the current methods of treatment for chancroid. (biomedcentral.com)
  • A monoclonal antibody raised against Haemophilus ducreyi was tested for its sensitivity and specificity as an immunofluorescence (IF) reagent using simulated vaginal smears containing H. ducreyi, smears taken from skin lesions of mice infected with H. ducreyi and patients from South Africa, Thailand and Malaysia with clinically diagnosed chancroid. (ox.ac.uk)
  • These results suggest that this antibody may provide a simple, rapid and sensitive means of detecting H. ducreyi in cases of chancroid. (ox.ac.uk)
  • Chancroid is a bacterial sexually transmitted infection (STI) caused by infection with Haemophilus ducreyi . (medscape.com)
  • Recently, the etiologic agent of chancroid, H ducreyi , has been isolated among chronic limb ulcers in the Asia Pacific region. (medscape.com)
  • Chancroid is caused by H ducreyi , a small, gram-negative, facultative anaerobic bacillus that is highly infective. (medscape.com)
  • Pathophysiological concept of Haemophilus ducreyi infection (chancroid). (medscape.com)
  • Chancroid is caused by a bacterium called Haemophilus ducreyi . (medlineplus.gov)
  • Haemophilus species including H. influenzae and H. ducreyi (chancroid). (medlineplus.gov)
  • Chancroid is cause by the bacteria Haemophilus ducreyi. (healthynewage.com)
  • Chancroid is an infection caused by the Streptobacillus Haemophilus ducreyi and transmitted by sexual activity. (blogarama.com)
  • Chancroid (soft chancre, ulcus molle) is a sexually transmitted disease caused by Haemophilus ducreyi. (symptoma.com)
  • 1 In contrast to these infections, many countries have achieved successful control of other STIs like chancroid (etiologic agent Haemophilus ducreyi) and lymphogranuloma venereum or LGV (etiologic agent Chlamydia trachomatis serovars L1, L2, and L3). (mhmedical.com)
  • A species of HAEMOPHILUS that appears to be the pathogen or causative agent of the sexually transmitted disease, CHANCROID . (bvsalud.org)
  • Chancroid is a sexually transmitted infection (STI) caused by the Haemophilus ducreyi bacteria. (condombreak.in)
  • Expression and structural diversity of the lipopolysaccharide of Haemophilus influenzae: implication in virulence. (semanticscholar.org)
  • Characterization of genetic and phenotypic diversity of invasive nontypeable Haemophilus influenzae. (ox.ac.uk)
  • The ability of unencapsulated (nontypeable) Haemophilus influenzae (NTHi) to cause systemic disease in healthy children has been recognized only in the past decade. (ox.ac.uk)
  • 24 Haemophilus influenzae. (ebookst.com)
  • Respiratory tract infections Pneumonia and other lower respiratory tract infections due to susceptible strains of Streptococcus pneumoniae , Haemophilus influenzae , Klebsiella pneumoniae and other organisms. (pillintrip.com)
  • Ophthalmic infections Due to susceptible strains of gonococci, staphylococci and Haemophilus influenzae. (pillintrip.com)
  • Pneumonia and other lower respiratory tract infections including those caused by susceptible strains of Streptococcus pneumoniae , Haemophilus influenzae , Klebsiella pneumoniae and other organisms. (pillintrip.com)
  • The global epidemiology of Haemophilus ducreyi infections microbiological diagnoses. (cdc.gov)
  • We determine the minimum inhibitory concentration (MIC) of Cinnamomum verum (cinnamon), Eugenia caryophyllus (clove) and Thymus satureioides (thyme) oil against 9 strains of H. ducreyi using the agar dilution method. (biomedcentral.com)
  • Antibiotic-resistant strains of H. ducreyi were equally susceptible to these 3 essential oils relative to non-resistant strains (p = 0.409). (biomedcentral.com)
  • Although no one strain of H. ducreyi demonstrates the wide range of and high degree of antibiotic resistance that is found in some bacteria, an increasing number of strains of H. ducreyi have developed some degree of antibiotic resistance[ 2 ]. (biomedcentral.com)
  • Haemophilus ducreyi Cutaneous Ulcer Strains Are Nearly Identical to Class I Genital Ulcer Strains. (medscape.com)
  • Most Haemophilus ducreyi strains are tolerant to tetracycline, amoxicillin and sulfamethoxazole/trimetoprim. (blogarama.com)
  • Structural studies of the cell envelope lipopolysaccharides from Haemophilus ducreyi strains ITM 2665 and ITM 4747. (semanticscholar.org)
  • Direct Whole-Genome Sequencing of Cutaneous Strains of Haemophilus ducreyi. (cdc.gov)
  • Haemophilus ducreyi is a gram negative coccobaccilus bacteria. (stepwards.com)
  • One such alternative that has shown promise in the treatment of other bacteria but has never been tested on H. ducreyi is the use of essential oils, a chemically diverse group of plant-derived compounds, many of which have antibacterial properties[ 6 ]. (biomedcentral.com)
  • L'ouvrage aborde âegalement les probláemes associâes áa l'apparition de souches de Neisseria gonorrhoeae et Haemophilus ducreyi râesistantes aux antimicrobiens, l'apparition de tendances dâemographiques et de nouveaux comportements sexuels propices áa la propagation des maladies sexuellement transmissibles et l'augmentation alarmante de la frâequence et de la gravitâe des complications qui peuvent avoir des râepercussions majeures sur la morbiditâe maternelle et infantile. (who.int)
  • Experimental Infection of Human Volunteers with Haemophilus ducreyi: Fifteen Years of Clinical Data and Experience. (medscape.com)
  • Outer membrane protein P4 is not required for virulence in the human challenge model of Haemophilus ducreyi infection. (medscape.com)
  • As a result of skin ulcers involving H. ducreyi . (cdc.gov)
  • Haemophilus ducreyi associated with skin ulcers among children, Solomon Islands. (symptoma.com)
  • Rapid detection of Haemophilus ducreyi in clinical and experimental infections using monoclonal antibody: a preliminary evaluation. (ox.ac.uk)
  • 2012, https://ecdc.europa.eu/en/publications-data/sexually-transmitted-infections-europe-1990-2010 (2012, accessed 9 March 2017). (who.int)
  • A fibrinogen-binding lipoprotein contributes to the virulence of Haemophilus ducreyi in humans. (medscape.com)
  • Evaluation of the repertoire of the TonB-dependent receptors of Haemophilus ducreyi for their role in virulence in humans. (medscape.com)
  • The enterobacterial common antigen-like gene cluster of Haemophilus ducreyi contributes to virulence in humans. (medscape.com)
  • With the goal of exploring possible alternative treatments, we tested essential oils (EOs) for their efficacy as antimicrobial agents against H. ducreyi . (biomedcentral.com)
  • Lundqvist A, Fernandez-Rodrigues J, Ahlman K, Lagergård T. Detoxified Haemophilus ducreyi cytolethal distending toxin and induction of toxin specific antibodies in the genital tract. (medscape.com)
  • H ducreyi is transmitted sexually by direct contact with purulent lesions and by autoinoculation to nonsexual sites, such as the eye and skin. (medscape.com)
  • Cole LE, Kawula TH, Toffer KL, Elkins C. The Haemophilus ducreyi serum resistance antigen DsrA confers attachment to human keratinocytes. (medscape.com)
  • The Haemophilus ducreyi LspA1 protein inhibits phagocytosis by using a new mechanism involving activation of C-terminal Src kinase. (medscape.com)
  • Host-pathogen interplay of Haemophilus ducreyi. (medscape.com)
  • It detected H. ducreyi in 95% of the animal lesions compared with 14% detected by culture. (ox.ac.uk)
  • Especie de HAEMOPHILUS que parece ser el patógeno o agente causal del CHANCROIDE, una enfermedad de transmisión sexual. (bvsalud.org)
  • H ducreyi penetrates the skin through breaks in the mucosal barriers and microabrasions on the skin. (medscape.com)
  • A are because the experience: Treatment removing although spots by to for Haemophilus ducreyi. (louis-adams.com)
  • Caused by HAEMOPHILUS DUCREYI , it occurs endemically almost worldwide, especially in tropical and subtropical countries and more commonly in seaports and urban areas than in rural areas. (nih.gov)
  • Haemophilus ducreyi inhibits phagocytosis by U-937 cells, a human macrophage-like cell line. (medscape.com)