Hematopoietic Cell Growth Factors: These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Epidermal Growth Factor: A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.Thymidine Phosphorylase: An enzyme that catalyzes the transfer of 2-deoxy-D-ribose from THYMIDINE to orthophosphate, thereby liberating thymidine.Vascular Endothelial Growth Factor A: The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.Endothelial Growth Factors: These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.Receptor, Epidermal Growth Factor: A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.Fibroblast Growth Factor 2: A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).Vascular Endothelial Growth Factors: A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Transforming Growth Factor beta: A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.Lymphokines: Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.Fibroblast Growth Factors: A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.Platelet-Derived Growth Factor: Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Transforming Growth Factors: Hormonally active polypeptides that can induce the transformed phenotype when added to normal, non-transformed cells. They have been found in culture fluids from retrovirally transformed cells and in tumor-derived cells as well as in non-neoplastic sources. Their transforming activities are due to the simultaneous action of two otherwise unrelated factors, TRANSFORMING GROWTH FACTOR ALPHA and TRANSFORMING GROWTH FACTOR BETA.Insulin-Like Growth Factor I: A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor.Hepatocyte Growth Factor: Multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. Its receptor is PROTO-ONCOGENE PROTEINS C-MET.Receptors, Growth Factor: Cell surface receptors that bind growth or trophic factors with high affinity, triggering intracellular responses which influence the growth, differentiation, or survival of cells.Transforming Growth Factor alpha: An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Nerve Growth Factors: Factors which enhance the growth potentialities of sensory and sympathetic nerve cells.Receptors, Vascular Endothelial Growth Factor: A family of closely related RECEPTOR PROTEIN-TYROSINE KINASES that bind vascular endothelial growth factors. They share a cluster of seven extracellular Ig-like domains which are important for ligand binding. They are highly expressed in vascular endothelial cells and are critical for the physiological and pathological growth, development and maintenance of blood and lymphatic vessels.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Nerve Growth Factor: NERVE GROWTH FACTOR is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth stimulating activity.Fibroblast Growth Factor 1: A 17-kDa single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. It binds to HEPARIN, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 2).Transforming Growth Factor beta1: A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.Receptors, Fibroblast Growth Factor: Specific molecular sites or structures on cell membranes that react with FIBROBLAST GROWTH FACTORS (both the basic and acidic forms), their analogs, or their antagonists to elicit or to inhibit the specific response of the cell to these factors. These receptors frequently possess tyrosine kinase activity.Intercellular Signaling Peptides and Proteins: Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.Neovascularization, Pathologic: A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Insulin-Like Growth Factor II: A well-characterized neutral peptide believed to be secreted by the LIVER and to circulate in the BLOOD. It has growth-regulating, insulin-like and mitogenic activities. The growth factor has a major, but not absolute, dependence on SOMATOTROPIN. It is believed to be a major fetal growth factor in contrast to INSULIN-LIKE GROWTH FACTOR I, which is a major growth factor in adults.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Stem Cell Factor: A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Receptors, Platelet-Derived Growth Factor: Specific receptors on cell membranes that react with PLATELET-DERIVED GROWTH FACTOR, its analogs, or antagonists. The alpha PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR ALPHA) and the beta PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA) are the two principle types of PDGF receptors. Activation of the protein-tyrosine kinase activity of the receptors occurs by ligand-induced dimerization or heterodimerization of PDGF receptor types.Receptor Protein-Tyrosine Kinases: A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Interleukin-3: A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.Fibroblast Growth Factor 7: A fibroblast growth factor that is a specific mitogen for EPITHELIAL CELLS. It binds a complex of HEPARAN SULFATE and FIBROBLAST GROWTH FACTOR RECEPTOR 2B.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Vascular Endothelial Growth Factor Receptor-2: A 200-230-kDa tyrosine kinase receptor for vascular endothelial growth factors found primarily in endothelial and hematopoietic cells and their precursors. VEGFR-2 is important for vascular and hematopoietic development, and mediates almost all endothelial cell responses to VEGF.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Hematopoiesis: The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Neovascularization, Physiologic: The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.Cell Line, Tumor: A cell line derived from cultured tumor cells.Interleukin-2: A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.Receptor, Fibroblast Growth Factor, Type 2: A fibroblast growth factor receptor that is found in two isoforms. One receptor isoform is found in the MESENCHYME and is activated by FIBROBLAST GROWTH FACTOR 2. A second isoform of fibroblast growth factor receptor 2 is found mainly in EPITHELIAL CELLS and is activated by FIBROBLAST GROWTH FACTOR 7 and FIBROBLAST GROWTH FACTOR 10. Mutation of the gene for fibroblast growth factor receptor 2 can result in craniosynostotic syndromes (e.g., APERT SYNDROME; and CROUZON SYNDROME).Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Receptor, Fibroblast Growth Factor, Type 1: A fibroblast growth factor receptor with specificity for FIBROBLAST GROWTH FACTORS; HEPARAN SULFATE PROTEOGLYCAN; and NEURONAL CELL ADHESION MOLECULES. Several variants of the receptor exist due to multiple ALTERNATIVE SPLICING of its mRNA. Fibroblast growth factor receptor 1 is a tyrosine kinase that transmits signals through the MAP KINASE SIGNALING SYSTEM.Hematopoietic System: The blood-making organs and tissues, principally the bone marrow and lymph nodes.Hematopoietic Stem Cell Transplantation: Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.Connective Tissue Growth Factor: A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It is found in hypertrophic CHONDROCYTES where it may play a role in CHONDROGENESIS and endochondral ossification.Piebaldism: Autosomal dominant, congenital disorder characterized by localized hypomelanosis of the skin and hair. The most familiar feature is a white forelock presenting in 80 to 90 percent of the patients. The underlying defect is possibly related to the differentiation and migration of melanoblasts, as well as to defective development of the neural crest (neurocristopathy). Piebaldism may be closely related to WAARDENBURG SYNDROME.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Vascular Endothelial Growth Factor Receptor-1: A 180-kDa VEGF receptor found primarily in endothelial cells that is essential for vasculogenesis and vascular maintenance. It is also known as Flt-1 (fms-like tyrosine kinase receptor-1). A soluble, alternatively spliced isoform of the receptor may serve as a binding protein that regulates the availability of various ligands for VEGF receptor binding and signal transduction.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Mice, Inbred C57BLReverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Mitogens: Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)Time Factors: Elements of limited time intervals, contributing to particular results or situations.Receptors, Transforming Growth Factor beta: Cell-surface proteins that bind transforming growth factor beta and trigger changes influencing the behavior of cells. Two types of transforming growth factor receptors have been recognized. They differ in affinity for different members of the transforming growth factor beta family and in cellular mechanisms of action.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Interleukin-4: A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Receptors, Mitogen: Glycoprotein molecules on the surface of B- and T-lymphocytes, that react with molecules of antilymphocyte sera, lectins, and other agents which induce blast transformation of lymphocytes.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Fibroblast Growth Factor 10: A fibroblast growth factor that is a mitogen for KERATINOCYTES. It activates FIBROBLAST GROWTH FACTOR RECEPTOR 2B and is involved in LUNG and limb development.Receptor, Platelet-Derived Growth Factor beta: A PDGF receptor that binds specifically to the PDGF-B chain. It contains a protein-tyrosine kinase activity that is involved in SIGNAL TRANSDUCTION.Endothelium: A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Proto-Oncogene Proteins c-kit: A protein-tyrosine kinase receptor that is specific for STEM CELL FACTOR. This interaction is crucial for the development of hematopoietic, gonadal, and pigment stem cells. Genetic mutations that disrupt the expression of PROTO-ONCOGENE PROTEINS C-KIT are associated with PIEBALDISM, while overexpression or constitutive activation of the c-kit protein-tyrosine kinase is associated with tumorigenesis.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Receptors, Nerve Growth Factor: Cell surface receptors that bind NERVE GROWTH FACTOR; (NGF) and a NGF-related family of neurotrophic factors that includes neurotrophins, BRAIN-DERIVED NEUROTROPHIC FACTOR and CILIARY NEUROTROPHIC FACTOR.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Angiogenesis Inducing Agents: Agents that induce or stimulate PHYSIOLOGIC ANGIOGENESIS or PATHOLOGIC ANGIOGENESIS.Mice, Inbred BALB CSomatomedins: Insulin-like polypeptides made by the liver and some fibroblasts and released into the blood when stimulated by SOMATOTROPIN. They cause sulfate incorporation into collagen, RNA, and DNA synthesis, which are prerequisites to cell division and growth of the organism.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Protein-Tyrosine Kinases: Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Insulin-Like Growth Factor Binding Proteins: A family of soluble proteins that bind insulin-like growth factors and modulate their biological actions at the cellular level. (Int J Gynaecol Obstet 1992;39(1):3-9)Receptor, IGF Type 1: A protein-tyrosine kinase receptor that is closely related in structure to the INSULIN RECEPTOR. Although commonly referred to as the IGF-I receptor, it binds both IGF-I and IGF-II with high affinity. It is comprised of a tetramer of two alpha and two beta subunits which are derived from cleavage of a single precursor protein. The beta subunit contains an intrinsic tyrosine kinase domain.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Kinetics: The rate dynamics in chemical or physical systems.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Receptors, Cell Surface: Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Heparin: A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.Drug Synergism: The action of a drug in promoting or enhancing the effectiveness of another drug.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Receptor, Fibroblast Growth Factor, Type 3: A fibroblast growth factor receptor that regulates CHONDROCYTE growth and CELL DIFFERENTIATION. Mutations in the gene for fibroblast growth factor receptor 3 have been associated with ACHONDROPLASIA; THANATOPHORIC DYSPLASIA and NEOPLASTIC CELL TRANSFORMATION.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Receptor, Platelet-Derived Growth Factor alpha: A PDGF receptor that binds specifically to both PDGF-A chains and PDGF-B chains. It contains a protein-tyrosine kinase activity that is involved in SIGNAL TRANSDUCTION.Antineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Proto-Oncogene Proteins c-met: Cell surface protein-tyrosine kinase receptors for HEPATOCYTE GROWTH FACTOR. They consist of an extracellular alpha chain which is disulfide-linked to the transmembrane beta chain. The cytoplasmic portion contains the catalytic domain and sites critical for the regulation of kinase activity. Mutations of the gene for PROTO-ONCOGENE PROTEINS C-MET are associated with papillary renal carcinoma and other neoplasia.Transforming Growth Factor beta2: A TGF-beta subtype that was originally identified as a GLIOBLASTOMA-derived factor which inhibits the antigen-dependent growth of both helper and CYTOTOXIC T LYMPHOCYTES. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta2 and TGF-beta2 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Umbilical Veins: Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Cell Adhesion: Adherence of cells to surfaces or to other cells.QuinazolinesPolymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.ThymidineAntigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Molecular Weight: The sum of the weight of all the atoms in a molecule.Colony-Forming Units Assay: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.Transplantation, Homologous: Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.Proto-Oncogene Proteins c-sis: Cellular DNA-binding proteins encoded by the sis gene (GENES, SIS). c-sis proteins make up the B chain of PLATELET-DERIVED GROWTH FACTOR. Overexpression of c-sis causes tumorigenesis.Tyrosine: A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.Protein-Serine-Threonine Kinases: A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.Proto-Oncogene Proteins c-akt: A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Mitogen-Activated Protein Kinases: A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Cell Growth Processes: Processes required for CELL ENLARGEMENT and CELL PROLIFERATION.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Interleukin-6: A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Hematoxylin: A dye obtained from the heartwood of logwood (Haematoxylon campechianum Linn., Leguminosae) used as a stain in microscopy and in the manufacture of ink.Receptor, Nerve Growth Factor: A low affinity receptor that binds NERVE GROWTH FACTOR; BRAIN-DERIVED NEUROTROPHIC FACTOR; NEUROTROPHIN 3; and neurotrophin 4.Breast Neoplasms: Tumors or cancer of the human BREAST.Transforming Growth Factor beta3: A TGF-beta subtype that plays role in regulating epithelial-mesenchymal interaction during embryonic development. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta3 and TGF-beta3 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Lung Neoplasms: Tumors or cancer of the LUNG.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Cell SeparationMast Cells: Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.Tetradecanoylphorbol Acetate: A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.Cell Lineage: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Culture Media, Serum-Free: CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Protein Kinase Inhibitors: Agents that inhibit PROTEIN KINASES.Interleukin-1: A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Receptor, trkA: A protein-tyrosine kinase receptor that is specific for NERVE GROWTH FACTOR; NEUROTROPHIN 3; neurotrophin 4, neurotrophin 5. It plays a crucial role in pain sensation and thermoregulation in humans. Gene mutations that cause loss of receptor function are associated with CONGENITAL INSENSITIVITY TO PAIN WITH ANHIDROSIS, while gene rearrangements that activate the protein-tyrosine kinase function are associated with tumorigenesis.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Wound Healing: Restoration of integrity to traumatized tissue.Cell Line, Transformed: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.MAP Kinase Signaling System: An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Transplantation Conditioning: Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.PC12 Cells: A CELL LINE derived from a PHEOCHROMOCYTOMA of the rat ADRENAL MEDULLA. PC12 cells stop dividing and undergo terminal differentiation when treated with NERVE GROWTH FACTOR, making the line a useful model system for NERVE CELL differentiation.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Growth Inhibitors: Endogenous or exogenous substances which inhibit the normal growth of human and animal cells or micro-organisms, as distinguished from those affecting plant growth (= PLANT GROWTH REGULATORS).Hematologic Neoplasms: Neoplasms located in the blood and blood-forming tissue (the bone marrow and lymphatic tissue). The commonest forms are the various types of LEUKEMIA, of LYMPHOMA, and of the progressive, life-threatening forms of the MYELODYSPLASTIC SYNDROMES.Spleen: An encapsulated lymphatic organ through which venous blood filters.Mice, SCID: Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.Interleukins: Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli.Insulin-Like Growth Factor Binding Protein 2: One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.Receptor, erbB-2: A cell surface protein-tyrosine kinase receptor that is overexpressed in a variety of ADENOCARCINOMAS. It has extensive homology to and heterodimerizes with the EGF RECEPTOR, the ERBB-3 RECEPTOR, and the ERBB-4 RECEPTOR. Activation of the erbB-2 receptor occurs through heterodimer formation with a ligand-bound erbB receptor family member.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Stromal Cells: Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.Receptors, Interleukin-2: Receptors present on activated T-LYMPHOCYTES and B-LYMPHOCYTES that are specific for INTERLEUKIN-2 and play an important role in LYMPHOCYTE ACTIVATION. They are heterotrimeric proteins consisting of the INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT, the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT, and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN.Insulin-Like Growth Factor Binding Protein 1: One of the six homologous proteins that specifically bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions. The function of this protein is not completely defined. However, several studies demonstrate that it inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions. (Proc Soc Exp Biol Med 1993;204(1):4-29)Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.In Situ Hybridization: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.PhosphoproteinsAdaptor Proteins, Signal Transducing: A broad category of carrier proteins that play a role in SIGNAL TRANSDUCTION. They generally contain several modular domains, each of which having its own binding activity, and act by forming complexes with other intracellular-signaling molecules. Signal-transducing adaptor proteins lack enzyme activity, however their activity can be modulated by other signal-transducing enzymesPeptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

*FLT3LG

1994). "Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells". ... and colony stimulating factor 1 (CSF-1). In synergy with other growth factors, Flt3 ligand stimulates the proliferation and ... For example, it is a major growth factor stimulating the growth of dendritic cells. GRCh38: Ensembl release 89: ENSG00000090554 ... "The membrane-bound isoform of stem cell factor synergizes with soluble flt3 ligand in supporting early hematopoietic cells in ...

*Interleukin 9

"Expression cloning of cDNA encoding a novel human hematopoietic growth factor: human homologue of murine T-cell growth factor ... "Il-9 stimulates release of chemotactic factors from human bronchial epithelial cells". American Journal of Respiratory Cell and ... IL-9 is produced by variety of cells like mast cells, NKT cells, Th2, Th17, Treg, ILC2, and Th9 cells in different amounts. ... "Cloning and expression of a cDNA for the human homolog of mouse T cell and mast cell growth factor P40". Cytokine. 2 (1): 9-12 ...

*HOXA6

... hematopoietic progenitor cells), under the regulation of growth factors and cell cycles. Interleukin 3 and all-trans retinoic ... This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene ... even had the capacity to transform primary hematopoietic cells into immortal cell lines. Transplantation of these cell lines ... In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C ...

*CD135

"Oncogenic signaling from the hematopoietic growth factor receptors c-Kit and Flt3". Cell. Signal. 21 (12): 1717-1726. doi: ... Drexler HG, Quentmeier H (2005). "FLT3: receptor and ligand". Growth Factors. 22 (2): 71-3. doi:10.1080/08977190410001700989. ... block FLT3-induced hematopoietic activity) are: TNF-Alpha (Tumor necrosis factor-alpha) TGF-Beta (Transforming growth factor- ... Signalling of FLT3 is important for the normal development of haematopoietic stem cells and progenitor cells. The FLT3 gene is ...

*PIM2 (gene)

"Mice Deficient for All PIM Kinases Display Reduced Body Size and Impaired Responses to Hematopoietic Growth Factors". Mol Cell ... Experiments done on nuclear factor κB (NFκB) nuclear translocation in human perineural invasion (PNI) revealed that an up- ... PIM2 or Proviral Integrations of Moloney virus 2 is serine/threonine kinase that has roles in cell growth, proliferation, ... Cell. 128 (2): 257-67. doi:10.1016/j.cell.2006.11.046. PMID 17254965. Yan B, Zemskova M, Holder S, Chin V, Kraft A, Koskinen PJ ...

*Gingival graft

A fibrin clot packed with blood-derived growth factors, extra cellular matrix and hematopoietic stem cells is fabricated and ... The limitation in quantity and the morbidity are the limiting factor of this technique. Allografting techniques (skin from ... autogenous growth factors and hematopoietic stem cells).[citation needed] A small amount of blood (10 millilitres (0.35 imp fl ... Blood-derived growth factors have been used in medicine and oral surgery for more than twenty years with an abundance of ...

*Interleuchina 3

... identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3., in Cell, vol. 47, nº 1, ... Urdal DL, Price V, Sassenfeld HM, et al., Molecular characterization of colony-stimulating factors and their receptors: human ... identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3, in Cell, vol. 47, nº 1, ... Cell. Biol., vol. 16, nº 6, UNITED STATES, giugno 1996, pp. 3035-46, ISSN 0270-7306 (WC · ACNP), PMID 8649415.. ...

*Myelofibrosis

The V617F mutation appears to make hematopoietic cells more sensitive to growth factors that need JAK2 for signal transduction ... Production of cytokines such as fibroblast growth factor by the abnormal hematopoietic cell clone (particularly by ... which is a reduction in the number of all blood cell types: red blood cells, white blood cells, and platelets. Red blood cells ... JAK2 mutations are significant because JAK2 plays a role in controlling production of blood cells from hematopoietic stem cells ...

*List of MeSH codes (D12.776)

... platelet factor 4 MeSH D12.776.467.374.200.750 - rantes MeSH D12.776.467.374.400.442 - hematopoietic cell growth factors MeSH ... vascular endothelial growth factor a MeSH D12.776.467.100.800.300 - vascular endothelial growth factor b MeSH D12.776.467.100. ... vascular endothelial growth factor c MeSH D12.776.467.100.800.500 - vascular endothelial growth factor d MeSH D12.776.467.100. ... fibroblast growth factor 4 MeSH D12.776.624.664.700.112 - fibroblast growth factor 6 MeSH D12.776.624.664.700.114 - fms-like ...

*Janus kinase 2

... appears to render hematopoietic cells more sensitive to growth factors such as erythropoietin and thrombopoietin, because the ... "Interaction of Janus kinases JAK-1 and JAK-2 with the insulin receptor and the insulin-like growth factor-1 receptor". ... is associated with Janus kinases and involved in signaling for cell growth and c-myc induction". Immunity. 6 (4): 449-57. doi: ... association with Jak2 in UT-7/Epo cells". Blood Cells, Molecules & Diseases. 26 (1): 15-24. doi:10.1006/bcmd.2000.0273. PMID ...

*Stem cell marker

... of a basic fibroblast growth factor-dependent multipotent hematopoietic cell line derived from embryonic stem cells". ... March 1997). "Factors predicting morbidity following hematopoietic stem cell transplantation". Bone Marrow Transplantation. 19 ... Hirao A, Arai F, Suda T (December 2004). "Regulation of cell cycle in hematopoietic stem cells by the niche". Cell Cycle. 3 (12 ... Stem cell markers are genes and their protein products used by scientists to isolate and identify stem cells. Stem cells can ...

*Interleukin 3

... identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell. 47 (1): 3-10. doi: ... "Molecular characterization of colony-stimulating factors and their receptors: human interleukin-3". Ann. N. Y. Acad. Sci. 554: ... He found a T cell derived factor that induced the synthesis of 20alpha-hydroxysteroid dehydrogenase in hematopoietic cells and ... identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell. 47 (1): 3-10. doi: ...

*Kenneth Kaushansky

Cell Cycle. 2006; 5:538-545 Kaushansky K. Lineage specific hematopoietic growth factors. The New England Journal of Medicine ... after acquiring the tools of blood cell colony growth, that I should purify a colony-stimulating factor (CSF), a substance that ... and emerged with a purified blood cell growth factor (GM-CSF), skills in both protein purification (separating one type of ... Kaushansky's lab at Stony Brook focuses on hematopoietic growth factors and the regulation of their gene expression. The lab ...

*Pre-metastatic niche

... vascular endothelial growth factor receptor 1) and stromal cells to these particular sites prior to the arrival of primary ... Tumor cells release factors that cause bone cells to resorb and therefore increase the rate of metastasis. Bone is conducive to ... Tumors metastasize to particular organs due to the migration of hematopoietic bone marrow cells expressing VEGFR1 ( ... Primary tumors recruit myeloid cells, which are the precursors to immune cells, in order to allow the tumor cells to avoid ...

*Transplantable organs and tissues

With the availability of the stem cell growth factors GM-CSF and G-CSF, most hematopoietic stem cell transplantation procedures ... 2003). "Pancreatic-derived factor (FAM3B), a novel islet cytokine, induces apoptosis of insulin-secreting beta-cells". Diabetes ... Hematopoietic stem cell transplantation (HSCT) is the transplantation of blood stem cells derived from the bone marrow (that is ... Hematopoietic stem cell transplantation remains a risky procedure with many possible complications; it has traditionally been ...

*DPF2

It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene ... functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. ... doi:10.1016/j.cell.2006.09.026. PMID 17081983. DPF2 protein, human at the US National Library of Medicine Medical Subject ... Cell Biol. 6 (2): 97-105. doi:10.1038/ncb1086. PMID 14743216. Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large- ...

*Oprelvekin

... growth factor that directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and ... IL-11 is a member of a family of human growth factors and is being produced in the bone marrow of healthy adults. Synonyms are ... The primary hematopoietic activity of Neumega is stimulation of megakaryocytopoiesis and thrombopoiesis. In mice and nonhuman ... Your doctors will ask you to undergo frequent blood cell counts to determine effects of therapy and the further course of ...

*Growth factor

Anti-apoptotic survival factor T-cell growth factor (TCGF) Thrombopoietin (TPO) Transforming growth factors Transforming growth ... Historically, cytokines were associated with hematopoietic (blood and lymph forming) cells and immune system cells (e.g., ... Growth factor for plasma cells. IL-7 - Growth factor for pre-B cells. Keratinocyte growth factor (KGF) Migration-stimulating ... Fibroblast growth factor 1(FGF1) Fibroblast growth factor 2(FGF2) Fibroblast growth factor 3(FGF3) Fibroblast growth factor 4( ...

*Granulocyte colony-stimulating factor

Stem cell transplantsEdit. G-CSF may also be given to the receiver in hematopoietic stem cell transplantation, to compensate ... "The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors". Proceedings of the ... growth factor activity. • granulocyte colony-stimulating factor receptor binding. Cellular component. • extracellular region. • ... hematopoietic system. G-CSF is also a potent inducer of hematopoietic stem cell (HSC) mobilization from the bone marrow into ...

*Lymph node stromal cell

In addition, lymph node stromal cells may produce growth factors which actively contribute to tumour cell metastasis. Malhotra ... B lymphocytes need a factor B cell activating factor (BAFF) for their survival, also produced by FDCs. Marginal reticular cells ... The primary B cell follicle matures in germinal centers. In the medulla are hematopoietic cells (which contribute to the ... dendritic cells move to the T cell zone or to the B cell follicle along the fibroblast reticular cell network. Dendritic cells ...

*Management of hair loss

"Induction of Hair Growth by Insulin-Like Growth Factor-1 in 1,763 MHz Radiofrequency-Irradiated Hair Follicle Cells". PLoS ONE ... along with fibroblast growth factors FGF7, FGF-2 and VEGF. β-catenin transcription increased, which promotes dermal papillae as ... The study did not show measurable systemic levels or hematopoietic side effects, suggesting potential for use in humans.[53] ... "Dietary isoflavone increases insulin-like growth factor-I production, thereby promoting hair growth in mice". The Journal of ...

*Endothelial stem cell

... vascular endothelial growth factors VEGF-C and VEGF-D function as ligands for the vascular endothelial growth factor receptor 3 ... Hematopoietic stem cells can of course undergo self-renewal, and are multipotent cells that give rise to erythrocytes (red ... which regulates cell growth and cell mobility. With uncontrolled beta-catenin, the cell loses its adhesive properties. As ECs ... It is well established that insulin-like growth factor (IGF) signaling is important for cell responses such as mitogenesis, ...

*Human genetic resistance to malaria

hematopoietic (stem cell) - the blood stem cells that give rise to all other blood cells ... Malaria remains a selective factor for the sickle cell trait.[28] Thalassemias[edit]. Main articles: Thalassemia and Alpha- ... virulence factors - enable an infectious agent to replicate and disseminate within a host in part by subverting or eluding host ... such as enhanced tolerance to disease mediated by HO-1 and reduced parasitic growth due to translocation of host micro-RNA into ...

*Osteoclast

... and growth factor interleukin 6 (IL-6). This last hormone, IL-6, is one of the factors in the disease osteoporosis, which is an ... RANKL activates NF-κβ (nuclear factor-κβ) and NFATc1 (nuclear factor of activated t cells, cytoplasmic, calcineurin-dependent 1 ... It may be important to note that while osteoclasts are derived from the hematopoietic lineage, osteoblasts are derived from ... Väänänen H, Zhao H, Mulari M, Halleen J (2000). "The cell biology of osteoclast function". J Cell Sci. 113 (3): 377-81. PMID ...
With the expression "bone marrow hematopoietic stem cell transplant" we intend a complex procedure used especially, but not only, in the treatment of leukimias and lymphomas. Stem cells can be obtained not only from bone marrow but also from peripheral blood after a specific preconditioning of the patient, or from umbilical-cord blood.. Indications for hematopoietic stem cell transplant are acute leukimias, chronic leukimias, different forms of bone marrow insufficiency, thalassemias, Hodgkin lymphoma, non Hodgkin lymphomas, myelomas, other chronic myeloproliferative diseases, numerous genetic disorders and, as a recent indication, some autoimmune illnesses.. ...
TY - JOUR. T1 - CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages. AU - Matsuoka, Sahoko. AU - Ebihara, Yasuhiro. AU - Xu, Ming Jiang. AU - Ishii, Takefumi. AU - Sugiyama, Daisuke. AU - Yoshino, Hiroshi. AU - Ueda, Takahiro. AU - Manabe, Atsushi. AU - Tanaka, Ryuhei. AU - Ikeda, Yasuo. AU - Nakahata, Tatsutoshi. AU - Tsuji, Kohichiro. PY - 2001/1/15. Y1 - 2001/1/15. N2 - The CD34 antigen serves as an important marker for primitive hematopoietic cells in therapeutic transplantation of hematopoietic stem cells (HSC) and gene therapy, but it has remained an open question as to whether or not most HSC express CD34. Using a competitive long-term reconstitution assay, the results of this study confirm developmental changes in CD34 expression on murine HSC. In fetuses and neonates, CD34 was expressed on Lin-c-Kit+ long-term repopulating HSC of bone marrow (BM), ...
HemoGenyx is a preclinical-stage biotechnology company focused on the discovery, development and commercialisation of novel therapies and treatments for blood diseases, like leukemia and lymphoma. The companys leading technologies aim to change the way in which bone marrow/hematopoietic stem cell (BM/HSC) transplants are performed and improve their efficacy.
TY - JOUR. T1 - Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. AU - North, Trista E.. AU - De Bruijn, Marella F T R. AU - Stacy, Terryl. AU - Talebian, Laleh. AU - Lind, Evan. AU - Robin, Catherine. AU - Binder, Michael. AU - Dzierzak, Elaine. AU - Speck, Nancy A.. PY - 2002. Y1 - 2002. N2 - Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1+/- embryos are heterogeneous and include CD45+ cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs ...
TY - JOUR. T1 - The repopulation potential of fetal liver hematopoietic stem cells in mice exceeds that of their adult bone marrow counterparts. AU - Rebel, Vivienne I.. AU - Miller, Cindy L.. AU - Eaves, Connie J.. AU - Lansdorp, Peter M.. PY - 1996/4/15. Y1 - 1996/4/15. N2 - Varying, limiting numbers of unseparated or purified cells (Ly-5.1), either from 14.5-day-old fetal liver (FL) or from adult bone marrow (BM) were coinjected with 105 unseparated BM cells (Ly-5.2) into lethally irradiated adult C57B1/6 recipients (Ly-5.2). The kinetics of donor cell repopulation of the lymphoid and myeloid compartments by Ly-5.1+ donor hematopoietic stem cells (ie, competitive repopulation units [CRU]) were monitored at various time points after the transplantation by Ly-5 analysis of the peripheral white blood cells (WBC). Recipients that had received on average less than 2 adult BM ...
The Wiskott Aldrich syndrome protein is an essential cytoskeleton regulator found in cells of the hematopoietic lineage and controlling the motility of leukocytes. The impact of WAS gene deficiency on the mobilization of hematopoietic progenitor/stem cells in circulation has remained unexplored but information would be pertinent in the context of autologous gene therapy of the Wiskott Aldrich syndrome disease. The response to granulocyte-colony stimulating factor mobilization was investigated in a murine WAS knock-out model of the disease, by measuring haematological parameters, circulation and engraftment of hematopoietic progenitor/stem cells. In the steady-state, adult WAS knock-out mice have B cell lymphopenia, marked neutrophilia, increased counts of circulating haematopoietic progenitor cells and a splenomegaly presumably caused by the retention of ...
TY - JOUR. T1 - Self-renewal of a purified Tie2+ hematopoietic stem cell population relies on mitochondrial clearance. AU - Ito, Kyoko. AU - Turcotte, Raphaël. AU - Cui, Jinhua. AU - Zimmerman, Samuel E.. AU - Pinho, Sandra. AU - Mizoguchi, Toshihide. AU - Arai, Fumio. AU - Runnels, Judith M.. AU - Alt, Clemens. AU - Teruya-Feldstein, Julie. AU - Mar, Jessica C.. AU - Singh, Rajat. AU - Suda, Toshio. AU - Lin, Charles P.. AU - Frenette, Paul S.. AU - Ito, Keisuke. PY - 2016/12/2. Y1 - 2016/12/2. N2 - A single hematopoietic stem cell (HSC) is capable of reconstituting hematopoiesis and maintaining homeostasis by balancing self-renewal and cell differentiation. The mechanisms of HSC division balance, however, are not yet defined. Here we demonstrate, by characterizing at the single-cell level a purified and minimally heterogeneous murine Tie2+ HSC population, that these top hierarchical HSCs preferentially ...
TY - JOUR. T1 - Functional association between expression of adhesion molecules and marrow repopulating potential of primitive murine hematopoietic progenitor cells (hpc). AU - Travcpff, C. M.. AU - Voder, M. C.. AU - Hiatt, K.. AU - Srour, Edward. PY - 1997. Y1 - 1997. N2 - A number of adhesion molecules have been implicated in the ability of transplanted hematopoietic stem cells (HSC) to engraft. We investigated whether the expression, or lack thereof, of CD11 a, CD43, CD44, CD49d, CD49e, and CD62L on Sca-1 + lin- cells augments or diminishes the bone marrow (BM) repoputatlng potential of these cells. A total of 103 Sca-l+ lin X+ or Sca-1+ lin X- cells (where X is any of the 6 molecules mentioned above) from B6.Hbbd congenic mice were competitively transplanted along with 3 x 104 C57/BI6 low density BM cells into fethally irradiated C57/BI6 recipients. In the event where ...
Hematopoietic stem/progenitor cells (HSPCs) maintain the hematopoietic system by balancing their self-renewal and differentiation events. Hematopoietic stem cells also migrate to various sites and interact with their specific microenvironment to maintain the integrity of the system. Rho GTPases have been found to control the migration of hematopoietic cells and other cell types. Although the role of RAC1, RAC2 and CDC42 has been studied, the role of RHOA in human hematopoietic stem cells is unclear. By utilizing constitutively active and dominant negative RHOA, we show that RHOA negatively regulates both in vitro and in vivo migration and dominant negative RHOA significantly increased the migration potential of human HSC/HPCs. Active RHOA expression favors the retention of hematopoietic stem/progenitor cells ...
Robust and large-scale expansion of umbilical cord blood stem cells in vitro is necessary for widening the usage of transplantation therapies for the treatment of hematological and immune diseases. The lack of understanding of the complex inter-cellular networks regulating stem cell fate in culture explains the low success met so far for the ex vivo expansion of hematopoietic stem cells. The development of a mathematical model of in vitro hematopoiesis coupled with gene expression profiling led to predictions about the secreted factors that play a crucial role in regulating hematopoietic stem cell self-renewal in culture. We tested 18 putative molecules predicted to display effects on primitive progenitor (Long-Term Culture-Initiating Cell; LTC-IC) output, functionally validating three stimulators (VEGF, PDGF, EGF) and three inhibitors (TGFβ, CCL4, CXCL10). ...
TY - JOUR. T1 - Mast cell growth factor (c-kit ligand) enhances cytokine stimulation of proliferation of the human factor-dependent cell line, M07e. AU - Hendrie, P. C.. AU - Miyazawa, K.. AU - Yang, Y. C.. AU - Langefeld, C. D.. AU - Broxmeyer, Hal. PY - 1991. Y1 - 1991. N2 - Murine mast cell growth factor (muMGF), a c-kit ligand, has additive to greater-than-additive effects on in vitro colony formation of murine and human myeloid progenitor cells stimulated with erythropoietin, granulocyte-macrophage colony-stimulating factor (GM-CSF), and/or interleukin (IL)-3. To confirm direct-acting effects on responding cells, MGF was assessed alone and in combination with other cytokines for effects on the proliferation of the human factor-dependent cell line, M07e. ...
Hematopoietic Stem Cell Biology is the newest installment in the Stem Cell Biology and Regenerative Medicine series, to which it adeptly contributes as it offers a selection of carefully chosen topics so that the readers can understand recent advances in the field of the hematopoietic stem cells and hemato/lymphopoiesis. Each chapter is not a simple review of the topic, but rather an in-depth insight, which allows the reader to attain a multifaceted knowledgebase on hematopoietic stem cells. The chapters, which are authored by the leading experts in the field, cover characteristics of the hematopoietic stem cells at the cellular and molecular levels, offers details in regulatory mechanisms of differentiation mechanisms of hematopoietic stem cells to more mature blood cell, and discusses ...
Hematopoietic Stem Cell Biology is the newest installment in the Stem Cell Biology and Regenerative Medicine series, to which it adeptly contributes as it offers a selection of carefully chosen topics so that the readers can understand recent advances in the field of the hematopoietic stem cells and hemato/lymphopoiesis. Each chapter is not a simple review of the topic, but rather an in-depth insight, which allows the reader to attain a multifaceted knowledgebase on hematopoietic stem cells. The chapters, which are authored by the leading experts in the field, cover characteristics of the hematopoietic stem cells at the cellular and molecular levels, offers details in regulatory mechanisms of differentiation mechanisms of hematopoietic stem cells to more mature blood cell, and discusses ...
Quiescent long-term hematopoietic stem cells (LT-HSCs) are efficiently activated by type I interferon (IFN-I). However, this effect remains poorly investigated in the context of IFN-I-inducing virus infections. Here we report that both vesicular stomatitis virus (VSV) and murine cytomegalovirus (MCMV) infection induce LT-HSC activation that substantially differs from the effects triggered upon injection of synthetic IFN-I-inducing agents. In both infections, inflammatory responses had to exceed local thresholds within the bone marrow to confer LT-HSC cell cycle entry, and IFN-I receptor triggering was not critical for this activation. After resolution of acute MCMV infection, LT-HSCs returned to phenotypic quiescence. However, non-acute MCMV infection induced a sustained inflammatory milieu within the bone marrow that was associated with long-lasting impairment of LT-HSC function. In conclusion, our results show that systemic virus infections fundamentally ...
The invasive fungal infection (IFI) is the most common cause of mortality related to autologous stem cell transplant. Taking into account that Saprophytic Aspergillus is usually acquired by inhalation, to protect the bronchial tree just before the tissue invasion is quite attractive. In haematologic patients, as well as those ones subjected to an Allogeneic haematopoietic progenitor cell transplant, there is another group of patients at high risk of Invasive Pulmonary Aspergillosis (IPA). These are those patients with acute myeloid leucemia (AML), submitted to induction, intensification or consolidation polychemotherapy. The IPA incidence rate in these patients, whenever during their evolution, reaches 18-20%, with usual treatments. Furthermore, unlike allogeneic haematopoietic progenitor cell transplant patients, neutropenia was the only IPA risk factor. Nowadays, pharmacologic prophylaxis against IPA, in patients with allogeneic ...
How is Stem Cell Growth Factor abbreviated? SCGF stands for Stem Cell Growth Factor. SCGF is defined as Stem Cell Growth Factor frequently.
According to a recently published report by Brisk Insights, the Global Hematopoietic Stem Cells Transplantation Market is expected to grow at the CAGR of 10.4 % during 2017-2025. The global hematopoietic stem cells transplantation market is segmented on the basis of application, transplant type, and geography. The report on global hematopoietic stem cells transplantation market (by application, transplant type, and geography) provides a detailed overview and predictive analysis of the market.. Full report available on Hematopoietic Stem Cells Transplantation Market: Global Industry Size, Growth, Share and Forecast to 2025 report at http://www.briskinsights.com/report/hematopoietic-stem-cells-transplantation-market. Market Insights. Increasing prevalence of cancer and anemia in both developed and developing ...
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a hematopoietic growth factor involved in the generation of granulocytes, macrophages, and dendritic cells from hematopoietic progenitor cells. We have recently demonstrated that GM-CSF has anti-apoptotic functions on neurons, and is neuroprotective in animal stroke models. The GM-CSF receptor α is expressed on adult neural stem cells in the rodent brain, and in culture. Addition of GM-CSF to NSCs in vitro increased neuronal differentiation in a dose-dependent manner as determined by quantitative PCR, reporter gene assays, and FACS analysis. Similar to the hematopoietic factor Granulocyte-colony stimulating factor (G-CSF), GM-CSF stimulates neuronal differentiation of adult NSCs. These data highlight the astonishingly similar functions of major ...
Acronyms and Abbreviations: AGM, aorta-gonad-mesonephros; BFU-E, burst-forming unit-erythroid; BFU-MK, burst-forming unit-megakaryocyte; CAFC, cobblestone area-forming cell; CAR, CXCL12-abundant reticular; CFC, colony-formingcell; CFU-E, colony-forming unit-erythroid; CFU-GM, colony-forming unit-granulocyte-macrophage; CFU-MK, colony-forming unit-megakaryocyte; CLP, common lymphoid progenitor; CMP, common myeloid progenitor; EBF, early B-cell factor; ECM, extracellular matrix; EGF, epidermal growth factor; EPO, erythropoietin; EPOR, erythropoietin receptor; FAK, focal adhesion kinase; FL, Flt-3 ligand; G-CSF, granulocyte colony-stimulating factor; G-CSF-R, granulocyte colony-stimulating factor receptor; GM-CSF, granulocyte-macrophage colony-stimulating factor; GM-CSF-R, granulocyte-monocyte colony-stimulating ...
The 40-fold increase in childhood megakaryocyte-erythroid and B-cell leukemia in Down syndrome implicates trisomy 21 (T21) in perturbing fetal hematopoiesis. Here, we show that compared with primary disomic controls, primary T21 fetal liver (FL) hematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors are markedly increased, whereas granulocyte-macrophage progenitors are reduced. Commensurately, HSC and megakaryocyte-erythroid progenitors show higher clonogenicity, with increased megakaryocyte, megakaryocyte-erythroid, and replatable blast colonies. Biased megakaryocyte-erythroid-primed gene expression was detected as early as the HSC compartment. In lymphopoiesis, T21 FL lymphoid-primed multipotential progenitors and early lymphoid progenitor numbers are maintained, but there was a 10-fold reduction in committed PreproB-lymphoid progenitors and the functional B-cell potential of HSC and early lymphoid progenitor is severely ...
The regulated lysosomal degradation pathway of autophagy prevents cellular damage and thus protects from malignant transformation. Autophagy is also required for the maturation of various hematopoietic lineages, namely the erythroid and lymphoid ones, yet its role in adult hematopoietic stem cells (HSCs) remained unexplored. While normal HSCs sustain life-long hematopoiesis, malignant transformation of HSCs or early progenitors leads to leukemia. Mechanisms protecting HSCs from cellular damage are therefore essential to prevent hematopoietic malignancies. By conditionally deleting the essential autophagy gene Atg7 in the hematopoietic system, we found that autophagy is required for the maintenance of true HSCs and therefore also of downstream hematopoietic progenitors. Loss of autophagy in HSCs leads to the expansion of a progenitor cell population in the ...
TY - JOUR. T1 - Effects of stem cell factor on osteoclast-like cell formation in long-term human marrow cultures. AU - Demulder, A.. AU - Suggs, S. V.. AU - Zsebo, K. M.. AU - Scarcez, T.. AU - Roodman, G. David. PY - 1992/11. Y1 - 1992/11. N2 - Stem cell factor (SCF) is a newly described hematopoietic growth factor that stimulates the growth of primitive hematopoietic progenitors and mast cells. Since the osteoclast precursor is hematopoietic in origin, we tested SCF for its capacity to stimulate the formation of osteoclast-like multinucleated cells (MNC) in long-term human marrow cultures. These MNC express an osteoclast phenotype and form resorption lacunae on calcified matrices. Addition of SCF alone (0.1 pg/ml to 100 ng/ml) to long-term marrow cultures ...
Umbilical cord blood (UCB) is an alternative hematopoietic stem cell (HSC) source that can ameliorate several diseases through transplantation. The purpose of this project is to analyze clinical studies comparing HSCs from a single cord blood unit (CBU) to HSCs from bone marrow, and to explore methods of increasing limited amounts of HSCs. It was found that UCB transplantation in adults is a viable method when a matched bone marrow transplant cannot be identified. Further clinical studies using two CBUs suggest better engraftment and lower risk of relapse. However, double cord blood transplantation has been faced with the challenge of single unit dominance in most studies. Ex vivo expansion of UCB HSCs is another promising method to overcome limited HSC counts.
The global Hematopoietic Stem Cells Transplantation (HSCT) Market is one amongst the enormously classified markets. The global Hematopoietic Stem Cells Transplantation (HSCT) market report offers information related to import and export, along with the current business chain in the market at the global level. It also delivers a plan regarding the expansion of supply and demand of the generated products and offering services compared with the key market players Kite Pharma, Thermo Fisher Scientific, CellGenix Technologie Transfer, Cesca Therapeutics, R&D Systems of the Hematopoietic Stem Cells Transplantation (HSCT) market globally.. Apply here for the sample copy of the report @: https://www.reportsbuzz.com/request-for-sample.html?repid=71502. A deep investigation of the Hematopoietic Stem Cells Transplantation (HSCT) market depends on global patterns, which ...
PURPOSE OF REVIEW: Hematopoietic stem cells (HSCs) possess two fundamental characteristics, the capacity for self-renewal and the sustained production of all blood cell lineages. The fine balance between HSC expansion and lineage specification is dynamically regulated by the interplay between external and internal stimuli. This review introduces recent advances in the roles played by the stem cell niche, regulatory transcriptional networks, and metabolic pathways in governing HSC self-renewal, commitment, and lineage differentiation. We will further focus on discoveries made by studying hematopoiesis at single-cell resolution. RECENT FINDINGS: HSCs require the support of an interactive milieu with their physical position within the perivascular niche dynamically regulating HSC behavior. In these microenvironments, transcription factor networks and nutrient-mediated regulation of energy resources, signaling ...
article{a4771b6c-1150-427f-a70a-c2fe50a441ba, abstract = {Diamond-Blackfan anemia (DBA) is a congenital bone marrow failure syndrome characterized by a specific deficiency in erythroid progenitors. Since some patients with DBA develop a reduction in thrombocytes and granulocytes with age, we asked whether multipotent hematopoietic progenitors from DBA patients had normal proliferative capacity in liquid expansion cultures. CD34(+) cells derived from DBA patients showed deficient proliferation in liquid culture containing IL-3, IL-6, and SCF. Single CD34(+) CD38(-) cells from DBA patients exhibited deficient proliferation recruitment in a limiting dilution assay containing IL-3, IL-6, SCF, Tpo, FIL, and G-CSF or containing IL-3, IL-6, and SCF. Our findings suggest that the underlying hematopoietic defect in DBA may not be limited to the erythroid lineage. Since a fraction of DBA patients have a deficiency in ribosomal protein S19 (RPS19), we ...
Bronchiolitis Obliterans (BO) is an obstructive lung disease that can affect individuals that have undergone a lung or hematopoietic stem cell transplant. BO has been studied most extensively in lung transplant recipients, where it is considered to represent chronic lung rejection. It is the leading cause of death after lung transplant, with mortality rates up to 55%. In hematopoietic stem cell transplantation, BO is thought to be a manifestation of chronic graft-vs-host disease (GVHD). Up to 45% of patients undergoing hematopoietic stem cell transplantation at the NHLBI develop a decline in pulmonary function. Conventional therapy for patients who develop BO consists of augmentation of systemic immunosuppressants. Systemic immunosuppression has limited efficacy for BO and is associated with deleterious consequences including increased risk of infections and decreased graft-versus tumor/leukemia effects.. ...
Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) ...
The newspaper (Yandu Rong media reporter Wang Jingtian correspondent Li Yiran) I think it is okay to participate in blood donation and donate hematopoietic stem cells. It is good for the community to do more things that are good for the body and mind. On December 27, Mr. Han of an iron and steel plant in a Tangshan iron and steel company successfully donated 183 ml of hematopoietic stem cell suspension in Shijiazhuang, the provincial capital, becoming the 9318th case of the Chinese bone marrow bank, the 440th case of the Hebei branch, and the first of the Tangshan Red Cross Society. 21 non-related hematopoietic stem cell donors. Up to now, nearly 9,000 citizens in Tangshan City have joined the China Bone Marrow Bank and 21 cases have been successfully donated. Simple and kind Master Han, a farmer, is a front-line worker at an ironworks in a steel company in Tangshan. He is simple and kind and enthusiastic. ...
Recombinant Human Stem Cell Factor produced ininsect cellsis a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is fused to a C-terminal His-tag and purified by proprietary chromatograp
Hematopoietic stem cells (HSCs) are the cells that can give rise to all different lineages of differentiated/mature blood cells. HSCs are capable of self-renewal and maintaining the homeostasis of differentiated cells for specific functions. The adult type HSCs were first identified in the para-aortic splanchnopleura/Aorta-Gonad-Mesonephros (PSp/AGM) region, the vitelline and umbilical arteries, yolk sac, and placenta. The current hypothesis is that HSCs differentiate from a "hemogenic endothelium". Core binding factors (CBFs), Runx1 and CBFβ, are essential for the generation of definitive hematopoiesis. We hypothesized that Runx1-CBFβ function is specifically required in the hemogenic endothelium per se for HSC emergence. To test this hypothesis, we selectively disrupted or supplied Runx1-CBFβ function in endothelial cells. Specifically, we conditionally deleted Runx1 in endothelial ...
TY - JOUR. T1 - Infectious complications following nonmyeloablative allogeneic hematopoietic stem cell transplantation. AU - Busca, Alessandro. AU - Locatelli, F.. AU - Barbui, A.. AU - Ghisetti, V.. AU - Cirillo, D.. AU - Serra, R.. AU - Audisio, E.. AU - Falda, M.. PY - 2003/9. Y1 - 2003/9. N2 - Nonmyeloablative hematopoietic stem cell transplantation (NST) has been explored in hematological malignancies and solid tumors in an attempt to minimize treatment-related toxicity. Whether this approach is associated with reduced risk of infectious complications is unclear. The aim of the current study was to evaluate the infectious complications in a series of 32 consecutive adult patients who received NSTat our institution. Peripheral blood stem cell grafts (n = 30) or marrow grafts (n = 2) were infused from human leukocyte antibody (HLA)·matched sibling (n = 30), partially matched related (n = 1), or unrelated (n = 1) donors. ...
Telomere length analysis of donor-derived bone marrow cells (Fig. 3) and HSCs (Fig. 4) shows substantial telomere shortening during serial HSC transplantation. The difference in mean TRF length (ΔTRF) of bone marrow cells after one round of HSC transplantation is ∼1.5 kb (Fig. 3 B). This agrees reasonably well with the predicted reduction in telomere size assuming that ΔTRF is mainly due to the minimum number of extra population doublings (∼12-13) required for expansion of the fraction of the transplanted HSC population which engraft to the size of the HSC pool in adult mice (∼3-5 × 104 cells; references 3, 4), and that the rate of telomere shortening during division of the transplanted HSCs is 50-100 bp per population doubling, as observed for other mouse cells 14,39,40. However, the extent of telomere shortening during the second round of HSC transplantation (ΔTRF ≈ 5.5 kb) is considerably greater than ΔTRF during the first round of ...
0054] In important embodiments of the invention, the umbilical cord blood cells are fractionated in order to generate enriched cell populations. As used herein, an enriched cell population is a cell population that has been manipulated in order to increase the frequency of a particular cell type in the population relative to the frequency of that cell type prior to manipulation. It is to be understood that the cell type being enriched is one that existed in the population prior to manipulation, and that enrichment results from the removal of other cell types from the population rather than addition of the cell type of interest. Of particular interest according to the invention are cell populations enriched in CD34+, CD133+, or CD34+/CD133+ cells. CD34 and CD133 are cell surface protein (or markers) that have ...
TY - JOUR. T1 - Rapid immune reconstitution following autologous hematopoietic stem cell transplantation in children. T2 - A single institution experience. AU - Hoepfner, S.. AU - Haut, Paul. AU - OGorman, M.. AU - Kletzel, M.. PY - 2003/2. Y1 - 2003/2. N2 - In this retrospective study, we review the immune reconstitution of children undergoing autologous hematopoietic stem cell transplantation. A total of 125 patients underwent autologous transplantation between 1992 and 2000. The report includes data on 58 patients. Data were not available on the remaining patients who either died before testing or data were not obtained. The parameters evaluated include: (a) immunophenotype by flow cytometry to quantify lymphocyte subpopulations (b) mitogen stimulation assays, and (c) quantitative immunoglobulins. The analysis reveals that CD3+ cells did not reach the normal range during the first year post-transplant. The median ...
TY - JOUR. T1 - Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells. AU - Van Os, R. AU - Van Schie, MLJ. AU - Willemze, R. AU - Fibbe, WE. PY - 2004/7. Y1 - 2004/7. N2 - Previous studies from our laboratory indicate that functional, mature neutrophils are essential for interleukin-8 (IL-8)-induced stem cell mobilization. To study a possible role of neutrophils in granulocyte colony-stimulating factor (G-CSF) induced hematopoietic mobilization, we assessed the number of circulating CD34(+) cells in healthy allogeneic stem cell donors on days 3, 4, and 5 of mobilization for comparison with the number of peripheral blood neutrophils and the plasma levels of IL-8, Flt3 ligand (FL), matrix metalloproteinase-9 ...
Hematopoietic stem cells (HSCs) are essential for the maintenance of the hematopoietic system. However, these cells cannot be maintained or created in vitro, and very little is known about their generation during embryogenesis. Many transcription factors and signaling pathways play essential roles at various stages of HSC development. Members of the ETS (E twenty-six) family of transcription factors are recognized as key regulators within the gene regulatory networks governing hematopoiesis, including the ontogeny of HSCs. Remarkably, although all ETS transcription factors bind the same DNA consensus sequence and overlapping tissue expression is observed, individual ETS transcription factors play unique roles in the development of HSCs. Also, these transcription factors are recurrently used throughout development and their functions are context-dependent, ...
TY - JOUR. T1 - Widespread nonhematopoietic tissue distribution by transplanted human progenitor cells with high aldehyde dehydrogenase activity. AU - Hess, David A.. AU - Craft, Timothy P.. AU - Wirthlin, Louisa. AU - Hohm, Sarah. AU - Zhou, Ping. AU - Eades, William C.. AU - Creer, Michael H.. AU - Sands, Mark S.. AU - Nolta, Jan A.. PY - 2008/3/1. Y1 - 2008/3/1. N2 - Transplanted adult progenitor cells distribute to peripheral organs and can promote endogenous cellular repair in damaged tissues. However, development of cell-based regenerative therapies has been hindered by the lack of preclinical models to efficiently assess multiple organ distribution and difficulty defining human cells with regenerative function. After transplantation into β-glucuronidase (GUSB)-deficient NOD/SCID/mucopolysaccharidosis type VII mice, we characterized the distribution of lineage-depleted human umbilical cord ...
TY - JOUR. T1 - Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples. AU - D'Alessio, F.. AU - Mirabelli, P.. AU - Gorrese, M.. AU - Scalia, G.. AU - Gemei, M.. AU - Mariotti, E.. AU - Di Noto, R.. AU - Martinelli, P.. AU - Fortunato, G.. AU - Paladini, D.. AU - Del Vecchio, L.. PY - 2011/1. Y1 - 2011/1. N2 - During the last decades, extended characterizations were performed of human full-term cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. First, we observed that the percentage of CD34 PosCD45 Dim cells was higher in hEPCB compared with hTCB and that it decreased during 16th-20th week of pregnancy. Within the CD34 PosCD45 Dim population, we examined the expression of ...
EV Morozova, YuYu Vlasova, MV Barabanshchikova, NN Mamaev, IM Barkhatov, AL Alyanskii, EI Darskaya, MV Vladovskaya, SN Bondarenko, IS Moiseev, BV Afanasyev RM Gorbacheva Scientific Research Institute of Pediatric Oncology, Hematology and Transplantation; IP Pavlov First Saint Petersburg State Medical University, 6/8 Lva Tolstogo str., Saint Petersburg, Russian Federation, 197022 For correspondence: Elena Vladislavovna Morozova, MD, PhD, 6/8 Lva Tolstogo str., Saint Petersburg, Russian Federation, 197022; e-mail: [email protected] For citation: Morozova EV, Vlasova YuYu, Barabanshchikova MV, et al. Chronic Myeloid Leukemia: Role of Allogeneic Hematopoietic Stem Cell Transplantation in the Era of Tyrosine Kinase Inhibitors. Clinical oncohematology. 2020;13(2):193-8 (In Russ). DOI: 10.21320/2500-2139-2020-13-2-193-198 ABSTRACT Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a radical method of chronic myeloid leukemia (CML) ...
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VA Balashova, VI Rugal, SS Bessmeltsev, SV Gritsaev, NYu Semenova, SV Voloshin, ZhV Chubukina, AV Shmidt, AD Garifullin, IM Zapreeva, AA Kuzyaeva, II Kostroma, AYu Kuvshinov, AV Chechetkin Russian Research Institute of Hematology and Transfusiology, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024 For correspondence: Valentina Andreevna Balashova, MD, PhD, 16 2-ya Sovetskaya str., Saint Petersburg, Russian Federation, 191024; Tel.: +7(812)717-19-37; e-mail: [email protected] For citation: Balashova VA, Rugal VI, Bessmeltsev SS, et al. Correlation of CD34+ Hematopoietic Stem Cells and CFU in Peripheral Blood Apheresis Products in Patients with Malignant Lymphoproliferative Diseases Before and After Cryopreservation Prior to auto-HSCT. Clinical oncohematology. 2018;11(4):368-77. DOI: 10.21320/2500-2139-2018-11-4-368-377 ABSTRACT Aim. To establish correlation between CD34+ autologous hematopoietic stem cell (HSC) count and ...
Osteoblasts are a key component in the regulation of the hematopoietic stem cell (HSC) niche. Manipulating osteoblast numbers results in a parallel change in HSC numbers. We tested the activity of strontium (Sr), a bone anabolic agent that enhances osteoblast function and inhibits osteoclast activity, on hematopoiesis. In vitro treatment of primary murine osteoblasts with Sr increased their ability to form bone nodules, and in vivo it increased osteoblast number, bone volume, and trabecular thickness and decreased trabecular pattern factor. However, the administration of Sr had no influence on primitive HSCs, although the number of hematopoietic progenitors was higher than in control cells. When Sr-treated mice were used as donors for HSC transplantation, no difference in the engraftment ability was observed, whereas hematopoietic recovery was delayed when they were used as recipients. Despite the changes in ...
Excluded therapies and medications, previous and concomitant:Anticancer chemotherapy or immunotherapy during the study or within 4 weeks prior to the first dose of the study drug; Radiotherapy for the treatment of a symptomatic (e.g. bone metastasis) as clinically indicated is allowed as long as it is not evidence of progressive disease (see 4.5.2); -Biological response modifiers, such as G-CSF, within 3 week prior to study entry. (G-CSF and other hematopoietic growth factors may be used in the management of acute toxicity such as febrile neutropenia when clinically indicated or at the discretion of the investigator; however they may not be substituted for a required dose reduction); Patients taking chronic erythropoietin are permitted provided no dose adjustment is undertaken within 2 months prior to the study or during the study; Investigational drug therapy outside of this trial during or within 4 weeks prior the screening assessment; Use ...
Use of biologic response modifiers, such as G-CSF, within 3 week of study entry. [G-CSF and other hematopoietic growth factors may be used in the management of acute toxicity such as febrile neutropenia when clinically indicated or at the discretion of the investigator, however they may not be substituted for a required dose reduction.] [Patients taking chronic erythropoietin are permitted provided no dose adjustment is undertaken within 2 months prior to the study or during the study ...
Infusion reactions are expected to occur and include nausea, vomiting, fever, rigors or chills, flushing, dyspnea, hypoxemia, chest tightness, hypertension, tachycardia, bradycardia, dysgeusia, hematuria, and mild headache. Premedication with antipyretics, histamine antagonists, and corticosteroids may reduce the incidence and intensity of infusion reactions. Severe reactions, including respiratory distress, severe bronchospasm, severe bradycardia with heart block or other arrhythmias, cardiac arrest, hypotension, hemolysis, elevated liver enzymes, renal compromise, encephalopathy, loss of consciousness, and seizure also may occur. Many of these reactions are related to the amount of DMSO administered. Minimizing the amount of DMSO administered may reduce the risk of such reactions, although idiosyncratic responses may occur even at DMSO doses thought to be tolerated. The actual amount of DMSO depends on the method of preparation of the product for infusion. Limiting the amount of DMSO infused ...
Infusion reactions are expected to occur and include nausea, vomiting, fever, rigors or chills, flushing, dyspnea, hypoxemia, chest tightness, hypertension, tachycardia, bradycardia, dysgeusia, hematuria, and mild headache. Premedication with antipyretic, histamine antagonists, and corticosteroids may reduce the incidence and intensity of infusion reactions.. Severe reactions, including respiratory distress, severe bronchospasm, severe bradycardia with heart block or other arrhythmias, cardiac arrest, hypotension, hemolysis, elevated liver enzymes, renal compromise, encephalopathy, loss of consciousness, and seizure also may occur. Many of these reactions are related to the amount of DMSO administered. Minimizing the amount of DMSO administered may reduce the risk of such reactions, although idiosyncratic responses may occur even at DMSO doses thought to be tolerated. The actual amount of DMSO depends on the method of preparation of the product for infusion. Limiting the amount of DMSO infused ...
TY - JOUR. T1 - Retention of stemness and vasculogenic potential of human umbilical cord blood stem cells after repeated expansions on PES-nanofiber matrices. AU - Joseph, Matthew. AU - Das, Manjusri. AU - Kanji, Suman. AU - Lu, Jingwei. AU - Aggarwal, Reeva. AU - Chakroborty, Debanjan. AU - Sarkar, Chandrani. AU - Yu, Hongmei. AU - Mao, Hai Quan. AU - Basu, Sujit. AU - Pompili, Vincent J.. AU - Das, Hiranmoy. PY - 2014/10. Y1 - 2014/10. N2 - Despite recent advances in cardiovascular medicine, ischemic diseases remain a major cause of morbidity and mortality. Although stem cell-based therapies for the treatment of ischemic diseases show great promise, limited availability of biologically functional stem cells mired the application of stem cell-based therapies. Previously, we reported a PES-nanofiber based ex vivo stem cell expansion technology, which supports expansion of human umbilical cord blood (UCB)-derived CD133+/CD34+ ...
TY - JOUR. T1 - Treatment of thrombotic microangiopathy after hematopoietic stem cell transplantation with recombinant human soluble thrombomodulin. AU - Fujiwara, Hideaki. AU - Maeda, Yoshinobu. AU - Sando, Yasuhisa. AU - Nakamura, Makoto. AU - Tani, Katsuma. AU - Ishikawa, Tatsunori. AU - Nishimori, Hisakazu. AU - Matsuoka, Ken-ichi. AU - Fujii, Nobuharu. AU - Kondo, Eisei. AU - Tanimoto, Mitsune. PY - 2015. Y1 - 2015. N2 - BACKGROUND: Transplant-associated thrombotic microangiopathy (TA-TMA) after hematopoietic stem cell transplantation (HSCT) remains a severe complication associated with underlying endothelial damage. TMA has a high mortality rate with no definite treatments and effective treatments are needed. STUDY DESIGN AND METHODS: The study objective was to retrospectively analyze the outcome of patients receiving recombinant human soluble thrombomodulin (rTM), which has cytoprotective effects against calcineurin inhibitor-induced ...
Blood, 2001; 98 (12) doi:. Authors: Zaucha J M, Gooley T, Bensinger W I, Heimfeld S, Chauncey T R Zaucha J M, Gooley T, Bensinger W I, Heimfeld S, Chauncey T R, Zaucha R, Martin P J, Flowers M E, Storek J, Georges G, Storb R, Torok-Storb B et al.(7) Affiliation: Fred Hutchinson Cancer Research Center, United States Abstract: A retrospective analysis of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cell (G-PBMC) products harvested from healthy donors indicates significant variability in both the absolute number and relative proportion of CD34, CD3, and CD14 cells obtained. This report examined whether variations in the cellular composition of G-PBMC products correlated with clinical outcomes after myeloablative allogeneic transplantation. The numbers of CD34, CD3, and CD14 cells infused into 181 human leukocyte antigen (HLA)-identical sibling recipients were analyzed with respect to ...
387558135 - EP 1127577 A3 2002-11-27 - Ligand for the c-kit receptor and methods of use thereof - [origin: US6159461A] Compositions comprising c-kit ligand, TNF- alpha , and a hematopoietic factor are useful to promote the expansion of peripheral blood cells or the expansion and differentiation of hematopoietic progenitor cells.[origin: US6159461A] Compositions comprising c-kit ligand, TNF- alpha , and a hematopoietic factor are useful to promote the expansion of peripheral blood cells or the expansion and differentiation of hematopoietic progenitor cells.
Murine acquired immunodeficiency syndrome (MAIDS) induced by defective LP-BM5 murine leukemia virus is a disease with many similarities to human AIDS. Previous studies indicated that the depressed hematopoiesis observed in LP-BM5-infected marrow cultures may be attributable to a defect of hematopoietic stroma. We report here the generation of permanent stromal cell lines from noninfected and LP-BM5-infected marrow cultures. Retrovirus infection was confirmed by polymerase chain reaction for viral genome. The ability of these cell lines to support in vitro hematopoiesis was studied. Results indicated that, when cocultured with normal or infected nonadherent mononuclear cells, noninfected cell lines efficiently supported the production of hematopoietic precursors, whereas viral-infected cell lines induced suppression of both normal and viral-infected progenitors. Expression of cytokine genes ...
Cytokine regulation of prethymic T-lymphoid progenitor-cell proliferation and/or differentiation has not been well-defined, although much is known of cytokine regulation of hemopoietic stem- and progenitor-cell development. Here we use a recently identified hemopoietic growth factor, stem-cell factor (SCF) (a form of the c-kit ligand), and a transplant model of thymocyte regeneration to assess the effect of SCF on the in vivo generation of prethymic, thymocyte progenitor-cell activity. We show that recombinant rat SCF (rrSCF164) administered to weanling rats selectively induces an increase in thymocyte progenitor activity in the spleens of treated rats as compared to rats treated with vehicle, polyethylene glycol (PEG)-conjugated rat albumin, or recombinant human granulocyte colony-stimulating factor (rhG-CSF). These data demonstrate that administration of ...
The murine stem cell virus (MSCV) promoter exhibits activity in mouse hematopoietic cells and embryonic stem cells. respectively. The strength of the GFP fluorescence in the body was comparable to the proportion of GFP-positive leukocytes. Moreover, the rate of recurrence of the GFP-expressing leukocytes was significantly correlated with the frequency of GFP-expressing Purkinje cells. These results suggest that the MSCV promoter is useful for preferentially expressing a transgene in Purkinje cells. In addition, the proportion of transduced leukocytes in the peripheral circulation reflects the expression level of the transgene in Purkinje Rabbit Polyclonal to PPP2R3B cells, which can be used as a way to monitor transgene expression properties in the cerebellum without invasive techniques. Introduction The Moloney murine leukemia virus (MoMLV)-based retrovirus vector has been widely used to ...
Hemopoietic stem cell factor (SCF), which is the ligand for the proto-oncogene c-kit receptor (allelic with W locus) and the product of Sl locus of the mouse, has recently been cloned. The human homologue has also been cloned, and recombinant protein (human rSCF) expressed and purified to homogeneity. To determine the effect of human rSCF in the presence or absence of human rIL-3 on human bone marrow-derived mast cells and basophils, human CD34+ pluripotent progenitor cells, highly enriched (greater than 99%) from bone marrow mononuclear cells, were cultured over agarose surfaces (interphase cultures) in the presence of human rIL-3, human rIL-3 and increasing concentrations of human rSCF, or human rSCF alone. Over 3 to 4 wk, human rSCF acted synergistically with human rIL-3 at all concentrations, producing a three- to fivefold increase in total, mast cell, and basophil numbers over human rIL-3 alone when ...
Oocyte control of granulosa and theca cell function may be mediated by several growth factors via a local feedback loop(s) between these cell types. This study examined both the role of oocyte-secreted factors on granulosa and thecal cells, cultured independently and in co-culture, and the effect of stem cell factor (SCF); a granulosa cell derived peptide that appears to have multiple roles in follicle development. Granulosa and theca cells were isolated from 2-6 mm healthy follicles of mature porcine ovaries and cultured under serum-free conditions, supplemented with: 100 ng/ml LR3 IGF-1, 10 ng/ml insulin, 100 ng/ml testosterone, 0-10 ng/ml SCF, 1 ng/ml FSH (granulosa), 0.01 ng/ml LH (theca) or 1 ng/ml FSH and 0.01 ng/ml LH (co-culture) and with/without oocyte conditioned medium (OCM) or 5 oocytes. Cells ...
TY - JOUR. T1 - Function, molecular structure and gene expression of stem cell factor (SCF). AU - Okada, S.. AU - Suda, T.. PY - 1992/8. Y1 - 1992/8. N2 - Mice of genotype W/Wv and Sl/Sld have been considered as a model of instinct hemopoietic disorders. W/Wv mice have a defect in hemopoietic stem cells and Sl/Sld mice have a defect in the microenvironment. The W locus in murine chromosome 5 encodes the c-kit proto-oncogene and the Sl locus in chromosome 10 encodes the ligand for c-kit, which has been named stem cell factor (SCF), mast cell growth factor (MGF), kit ligand (KL) and steel factor (SL). The cDNA sequence of SCF suggest that it is synthesized as an integral transmembrane protein and that it has common tertiary structure with M-CSF. SCF enhances the proliferation of hemopoietic stem cells and progenitor ...
colony-stimulating factor, focal cerebral-ischemia, transient global-ischemia, endothelial growth-factor, newly generated neurons, central-nervous-system, long-term survival, neural stem-cells, rat dentate gyrus, hippocampal-neurons ...
Hemopoietic growth factors regulate the differentiation and proliferation of particular progenitor cells. Made available through recombinant DNA technology, they hold tremendous potential for medical uses when a persons natural ability to form blood cells is diminished or defective. Recombinant erythropoietin (EPO) is very effective in treating the diminished red blood cell production that accompanies end-stage kidney disease. Erythropoietin is a sialoglycoprotein hormone produced by peritubular cells of kidney Granulocyte-macrophage colony-stimulating factor and granulocyte CSF are given to stimulate white blood cell formation in cancer patients who are receiving chemotherapy, which tends to kill their red bone marrow cells as well as the cancer cells. Thrombopoietin shows great promise for preventing platelet depletion ...
Interactions between products of the mouse W locus, which encodes the c-kit tyrosine kinase receptor, and the Sl locus, which encodes a ligand for c-kit receptor, which we have designated stem cell factor (SCF), have a critical role in the development of mast cells. Mice homozygous for mutations at either locus exhibit several phenotypic abnormalities including a virtual absence of mast cells. Moreover, the c-kit ligand SCF can induce the proliferation and maturation of normal mast cells in vitro or in vivo, and also can result in repair of the mast cell deficiency of Sl/Sld mice in vivo. We now report that administration of SCF intradermally in vivo results in dermal mast cell activation and a mast cell-dependent acute inflammatory response. This effect is c-kit receptor dependent, in that it is not observed when SCF is administered to mice containing dermal mast ...
Growth factors and transcription factors are well known to regulate pluripotent stem cells, but less is known about translational control in stem cells. Here, we use embryonic stem cells (ESCs) to investigate a connection between ESC growth factors and eIF2α-mediated translational control (eIF2α phosphorylation promotes protein expression from mRNAs with upstream open-reading frames, or uORFs). We find abundant phosphorylated P-eIF2α (P-eIF2α) in both pluripotent mouse and human ESCs, but little P-eIF2α in ESCs triggered to differentiate. We show that the growth factors LIF (leukemia inhibitory factor) and BMP4 (bone morphogenic protein 4) both maintain P-eIF2α in mESCs, but use distinct mechanisms: LIF inhibits an eIF2α phosphatase whereas BMP4 activates an eIF2α kinase. ...
Journal of Immunology. 178(6):3511-3520. PubMed , PDF , ABSTRACT. The hemopoietic microenvironment consists of a diverse repertoire of cells capable of providing signals that influence hemopoietic stem cell function. Although the role of osteoblasts and vascular endothelial cells has recently been characterized, the function of the most abundant cell type in the bone marrow, the adipocyte, is less defined. Given the emergence of a growing number of adipokines, it is possible that these factors may also play a role in regulating hematopoiesis. Here, we investigated the role of adiponectin, a secreted molecule derived from adipocytes, in hemopoietic stem cell (HSC) function. We show that adiponectin is expressed by components of the HSC niche and its receptors AdipoR1 and AdipoR2 are expressed by HSCs. At a functional level, adiponectin influences HSCs by increasing their proliferation, while retaining the ...
In legal setback for FDA, In landmark step judge does not defer to the agency in key fat stem cell clinic chain court case, orders full trial https://ipscell.com/2020/01/in-landmark-step-judge-does-not-defer-to-fda-in-stem-cell-clinic-chain-case-orders-full-trial/ ...
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TY - JOUR. T1 - Intrinsic Hematopoietic Stem Cell/Progenitor Plasticity. T2 - Inversions. AU - Colvin, Gerald A.. AU - Lambert, Jean François. AU - Moore, Brian E.. AU - Carlson, Jane E.. AU - Dooner, Mark S.. AU - Abedi, Mehrdad. AU - Cerny, Jan. AU - Quesenberry, Peter J.. PY - 2004/4. Y1 - 2004/4. N2 - Traditional concepts indicate that stem cells give rise to progenitor cells in a hierarchical system. We studied murine engraftable stem cells (ESCs) and progenitors in in vitro and found that ESC and progenitors exist in a reversible continuum, rather then a hierarchy. B6.SJL and BALB/c marrow cells were serially cultured with thrombopoietin (TPO), FLT-3 ligand (FLT-3L), and steel factor through cell cycle. Progenitors (high-proliferative potential colony-forming cells (HPP-CFC) and colony-forming unit culture (CFU-c)) and ESC capacity was determined. The ...
To investigate the functional properties of IL-2 and TNF alpha on leukemic B cells, we evaluated (1) the regulation of expression of TNF receptors (TNF-R) and IL-2 receptors on leukemic B cells after culture with TNF alpha and IL-2; (2) the effect of the combination of TNF alpha and IL-2 in a proliferative in vitro assay; and (3) the expression and regulation by these cytokines of receptors for hematopoietic factors, including IL-3, granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF ...
Expression increases with inflammation or iron overload, and decreases with anemia or hypoxia.. Hepcidin exerts its effects by binding to the iron efflux protein, ferroportin, and inducing its internalization and degradation. This interaction inhibits absorption of dietary iron and inhibits release of storage iron, thus resulting functional iron deficiency.. Inflammation can also contribute to anemia via other mechanisms. For example, inflammation has been shown to cause decreased erythrocyte survival in some species; some studies have implicated inflammatory cytokines as inhibitors of erythropoiesis via direct toxic effects on erythroid precursors, decreased expression of hematopoietic factors, or decreased expression of erythropoitin receptors; and some investigators have suggested that oxidants produced by activated neutrophils cause altered erythrocyte surface antigenicity, in turn lead to accelerated immunological destruction of erythrocytes.. Absolute Iron Deficiency. ...
Macrophage Colony Stimulating Factor (M-CSF) ,also known as CSF1,is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The active form of the protein is found extracellularly as a disulfide-link...
CiteWeb id: 19900000258. CiteWeb score: 2446. DOI: 10.1073/pnas.87.18.6934. A family of cytokine receptors comprising molecules specific for a diverse group of hematopoietic factors and growth hormones has been principally defined by a striking homology of binding domains. This work proposes that the approximately 200-residue binding segment of the canonical cytokine receptor is composed of two discrete folding domains that share a significant sequence and structural resemblance. Analogous motifs are found in tandem approximately 100-amino acid domains in the extracellular segments of a receptor family formed by the interferon-alpha/beta and -gamma receptors and tissue factor, a membrane tether for a coagulation protease. Domains from the receptor supergroup reveal clear evolutionary links to fibronectin type III structures, approximately 90-amino acid modules that are typically found in ...
A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished. Type 1 T helper cells (TH1) produced IL 2, interferon-gamma, GM-CSF, and IL 3 in response to antigen + presenting cells or to Con A, whereas type 2 helper T cells (TH2) produced IL 3, BSF1, and two other activities unique to the TH2 subset, a mast cell growth factor distinct from IL 3 and a T cell growth factor distinct from IL 2. Clones representing each type of T cell were characterized, and the pattern of lymphokine activities was consistent within each set. The secreted proteins induced by Con A were analyzed by biosynthetic labeling and SDS gel electrophoresis, and significant differences were seen ...
Yung, Y and Moore, M A., "Long-term in vitro culture of murine mast cells. Iii. Discrimination of mast cell growth factor and granulocyte-csf." (1982). Subject Strain Bibliography 1982. 4470 ...
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KIT encodes the mast/stem cell growth factor tyrosine kinase receptor. The heterozygous W mouse phenotype is similar to the human piebald trait, also caused by a KIT mutation, which is characterized by a congenital white hair forelock and ventral and extremity depigmentation (Fleischman et al. 1991). Mutations in coding or regulation of KIT have been characterized in additional species as causative of defects in pigmentation and hearing (Haase et al. 2007; Ruan et al. 2005; Spritz and Beighton 1998). Cable et al. (1995) have demonstrated that mutations in Kit do not prevent early melanoblast migration or differentiation in mice white spotting mutants but severely affect melanoblast survival during embryonic development.. Cairns et al. (2003) described murine cell type-specific DNase I hypersensitive sites that delineated Kit regulatory regions in primordial germ cells, hematopoietic stem ...
Alternative Name. KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT, piebald trait; mast/stem cell growth factor receptor Kit; C Kit; CD117; SCFR; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; PBT; C-Kit;. ...
RCC treatment has been classically derived from clinical trials that incorporated all histologies comprising clear cell, papillary, chromophobe, and other rarer subtypes. Most recently, novel therapies (sunitinib and sorafenib) have shown significant clinical activity in advanced RCC and have changed the standard of care in this disease [1-3]. However, pivotal studies with these drugs were performed exclusively in patients with clear cell histology. Therefore, the optimal therapy for papillary and chromophobe histologies remains unknown. Overall, ChRCC is considered to portend a good prognosis and is associated with earlier-stage tumors and longer overall survival in comparison with CCRCC. There are conflicting reports on prognosis in metastatic disease, and few data on response to treatment exist. Increased VEGF-6 and c-Kit (i.e., mast/stem cell growth factor receptor; proto-oncogene c-Kit; tyrosine-protein ...
Receptor faktora rasta mastocita/matičnih ćelija (engl. Mast/stem cell growth factor receptor - SCFR), takođe poznat kao proto-onkogen c-Kit ili tirozinska proteinska kinaza Kit ili CD117, je protein koji je kod ljudi kodiran KIT genom.[1] Višestruke transkriptne varijante koje kodiraju različite izoforme ovog gena su poznate.[2] KIT prvi opisao nemački biohemičar Aksel Ulrič 1987. kao ćelijski homolog mačijeg sarkomnog viralnog onkogena v-kit.[3]. ...
The human stem cell factor (SCF) is a crucial growth factor for mast cells in the dermis and for the melanocytes in the basal layers of the epidermis. SCF is produced, among others, by keratinocytes. This study examines the possible regulation of the expression of SCF from keratinocytes by all-trans retinoic acid (RA) and dexamethasone in vitro by the keratinocyte cell line HaCaT. The HaCaT-cells were incubated for 24 hours or 11 days, respectively, with one of the above mentioned substances (10 to the power of -5 M to 10 to the power of -9 M). The analysis of the number of HaCaT-cells, of the total SCF protein, its splice variants (mSCF, sSCF), the receptors of RA (RAR-alpha, -beta, -gamma), and of the dexamethasone (GR-alpha, -beta) was done by ELISA and RT-PCR. The following results were found: RA induces an increase of SCF, dexamethasone at a short ...
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Stem cell factor (SCF, also called Steel factor or Kit ligand) is a growth factor promoting proliferation, migration, survival, and differentiation of hematopoietic progenitors, melanocytes, and germ cells.
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1812661DNAHomo sapiens 1cggcacgagg gcccagagga ataagttaac cttggtgcct gcgtccgtga gaattcagca 60tggaatgtct ctactatttc ctgggatttc tgctcctggc tgcaagattg ccacttgatg 120ccgccaaacg atttcatgat gtgctgggca atgaaagacc ttctgcttac atgagggagc 180acaatcaatt aaatggctgg tcttctgatg aaaatgactg gaatgaaaaa ctctacccag 240tgtggaagcg gggagacatg aggtggaaaa actcctggaa gggaggccgt gtgcaggcgg 300tcctgaccag tgactcacca gccctcgtgg gctcaaatat aacatttgcg gtgaacctga 360tattccctag atgccaaaag gaagatgcca atggcaacat agtctatgag aagaactgca 420gaaatgaggc tggtttatct gctgatccat atgtttacaa ctggacagca tggtcagagg 480acagtgacgg ggaaaatggc accggccaaa gccatcataa cgtcttccct gatgggaaac 540cttttcctca ccaccccgga tggagaagat ggaatttcat ctacgtcttc cacacacttg 600gtcagtattt ccagaaattg ggacgatgtt cagtgagagt ttctgtgaac acagccaatg 660tgacacttgg gcctcaactc atggaagtga ctgtctacag aagacatgga cgggcatatg 720ttcccatcgc acaagtgaaa gatgtgtacg tggtaacaga tcagattcct gtgtttgtga 780ctatgttcca gaagaacgat cgaaattcat ccgacgaaac cttcccaaag atctccccat 840tatgtttgat gtcctgattc ...
Transcription of stem cell factor (SCF) is potentiated by glucocorticoids and interleukin-1ß through concerted regulation of a GRE-like and an NF-κB response ...
Cancer stem cells (CSC) are a rare subpopulation of undifferentiated cells that are responsible for tumor initiation and tumor regeneration after chemotherapy. Although a universal marker for CSCs has not been identified, previous studies showed that c-kit (CD117) functioned as a stem cell factor (SCF) receptor, and SCF-ckit signaling axis was essential for self-renewal and proliferation of lung CSCs. During tumorigenesis, subsets of tumor cells disseminate from primary tumors by undergoing phenotypic changes that allow the cells to penetrate blood vessels. These changes are accompanied by a process described as epithelial-mesenchymal transition (EMT). EMT endows epithelial cells with enhanced invasive potential by the loss of their epithelial characteristics and the acquisition of a mesenchymal phenotype. The aim of this study is to correlate c-kit-positive CSCs and EMT-positive, ...
Kit Component:- KN303427G1, Clec11a gRNA vector 1 in pCas-Guide vector- KN303427G2, Clec11a gRNA vector 2 in pCas-Guide vector- KN303427D, donor…
Mouse SCF is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells.
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the…
Filgastrim (granulocyte colony stimulating factor or G-CSF) is a multi-modal hematopoietic growth factor used routinely in the hematology/oncology setting but r...
SCGF antibody [6D5] (C-type lectin domain family 11, member A) for IHC, WB. Anti-SCGF mAb (GTX52987) is tested in Human samples. 100% Ab-Assurance.
Investigation of the role of target cell factors in retrovirus transduction. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
10 20 30 40 , , , , 1 .....MTQETALGAALKSAV...QT.MS.KKKQTEMIADHIYGKYD.VFKR.FKPL....ALGI. 2 .....------------MTE...KL.KN.SKEVIAYIAECFPKCFT.LEGE.AKPL....KIGI. 3 .....------------MTE...KL.KN.SKEVIAYIAECFPKCFT.LEGE.AKPL....KIGI. 4 .....----------MENTE...KL.KN.SKEVIAYIAECFPKCFT.LEGE.AKPL....KIGI. 5 .....----------MENTE...KL.KN.SKEVIAYVAECFPKCFT.LEGE.AKPL....KIGI. 6 ml...AGRPLKPGQLMTE--...KL.KN.SKEVIAYIAECFPKCFT.LEGE.AKPL....KIGI. 7 .....----------MENTE...KL.KN.SKEVIAYIAECFPNCFT.LEGE.AKPL....KIGI. 8 .....----------MENQP...KL.NS.SKEVIAFLAERFPLCFT.TEGE.TRPL....KIGI. 9 .....----------MENQP...KL.NS.SKEVIAFLAERFPHCFS.AEGE.ARPL....KIGI. 10 .....----------MENSE...KL.AN.SKEVIAYIAERFPKCFI.LEGE.AKPL....KIGI. 11 .....----------MENQP...KL.NS.SKEVIAFLAERFPLCFT.AEGE.ARPL....KIGI. 12 .....----------MENQP...KL.NS.SKEVIAFLAERFPLCFT.AEGE.ARPL....KIGI. 13 .....----------MENQP...KL.NS.SKEVIAFLAERFPLCFT.AEGE.ARPL....KIGI. 14 .....----------MENQP...KL.NS.SKEVIAFLAERFPLCFT.AEGE.ARPL....KIGI. 15 ...
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
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Hi! For a long time Ive got problems with heart palpitations, so I started to ingest magnesium, because the family doctor suggested that it may be magnesium deficiency, so I take a very long time th...

Endogenous Retrovirus Insertion in the KIT Oncogene Determines White and White spotting in Domestic Cats | G3: Genes | Genomes ...Endogenous Retrovirus Insertion in the KIT Oncogene Determines White and White spotting in Domestic Cats | G3: Genes | Genomes ...

KIT encodes the mast/stem cell growth factor tyrosine kinase receptor. The heterozygous W mouse phenotype is similar to the ... Hematopoietic and mast cell analysis. Hematopoietic profiles of two pigmented and two white deaf cats were generated by Antech ... suggestive of additional genetic modifying factors. ... or hepatocyte growth factor (HGF) than by KIT (Aoki et al. 2009 ... 2003 Kit regulatory elements required for expression in developing hematopoietic and germ cell lineages. Blood 102: 3954-3962. ...
more infohttp://www.g3journal.org/content/4/10/1881.full

B-Cell Lymphoma Medication: Hematopoietic Growth Factors, Monoclonal Antibodies, Corticosteroids, Antineoplastic Agents,...B-Cell Lymphoma Medication: Hematopoietic Growth Factors, Monoclonal Antibodies, Corticosteroids, Antineoplastic Agents,...

Most (ie, 80-90%) NHLs are of B-cell origin. ... by the international follicular lymphoma prognostic factor ... Hematopoietic Growth Factors. Class Summary. These agents can induce an increase in reticulocyte counts, with a subsequent ... T cell therapy is a form of adoptive T-cell therapy in which T cells are genetically engineered to express a CAR. CAR T cells ... As with filgrastim, it acts on hematopoietic cells by binding to specific cell surface receptors, thereby activating and ...
more infohttps://emedicine.medscape.com/article/202677-medication

Ex Vivo Expansion of Primitive Hematopoietic Cells by Using Hematopoietic Growth FactorsEx Vivo Expansion of Primitive Hematopoietic Cells by Using Hematopoietic Growth Factors

... granulocyte-colony stimulating factor (G-CSF). To find optimal combination of growth factors, CD34 cells were cultured for 9 ... CONCLUSION: Primitive hematopoietic cells can be successfully expanded ex vivo by using hematopoietic growth factors. Duration ... Ex Vivo Expansion of Primitive Hematopoietic Cells by Using Hematopoietic Growth Factors. ... In this study, CD34 cells were cultured with various hematopoietic growth factors which are known to stimulate proliferation of ...
more infohttps://koreamed.org/article/1093KJPHO/2001.8.1.110

Vascular Endothelial Growth Factor Inhibits the Development of Dendritic Cells and Dramatically Affects the Differentiation of...Vascular Endothelial Growth Factor Inhibits the Development of Dendritic Cells and Dramatically Affects the Differentiation of...

Endothelial cells produce several hematopoietic growth factors such as SCF, granulocyte-macrophage colony-stimulating factor ( ... Myeloid progenitor cells regulatory effects of vascular endothelial cell growth factor. Int J Hematol 1995;62:203. ... BM cells were cultured for 14 days in semisolid methylcellulose medium supplemented with growth factors supporting growth of ... Vascular endothelial growth factor affects dendritic cell maturation through the inhibition of nuclear factor-kB activation in ...
more infohttp://www.bloodjournal.org/content/92/11/4150?ijkey=bbecb0adf177b009e8dc10639b87ca4fe47d5fdc&keytype2=tf_ipsecsha&sso-checked=true

Vascular Endothelial Growth Factor Inhibits the Development of Dendritic Cells and Dramatically Affects the Differentiation of...Vascular Endothelial Growth Factor Inhibits the Development of Dendritic Cells and Dramatically Affects the Differentiation of...

Endothelial cells produce several hematopoietic growth factors such as SCF, granulocyte-macrophage colony-stimulating factor ( ... Myeloid progenitor cells regulatory effects of vascular endothelial cell growth factor. Int J Hematol 1995;62:203. ... BM cells were cultured for 14 days in semisolid methylcellulose medium supplemented with growth factors supporting growth of ... Vascular endothelial growth factor affects dendritic cell maturation through the inhibition of nuclear factor-kB activation in ...
more infohttp://www.bloodjournal.org/content/92/11/4150?ijkey=c67ab754584dd5c4029e722daf3f7b30d4d97144&keytype2=tf_ipsecsha&sso-checked=true

Transduction of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand into Hematopoietic Cells Leads to Inhibition of...Transduction of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand into Hematopoietic Cells Leads to Inhibition of...

The growth factors murine interleukin (IL)-3, murine IL-6, and murine stem cell factor were supplied by PeproTech, Inc. (Rocky ... The cells were replaced with RPMI 1640 growth medium for 3 days. The cells were detected by measuring the sub-G1 apoptotic cell ... NK cells, B cells, macrophages, monocytes, and dendritic cells. However, among those cell types, only murine NK cells ... These cells include natural killer (NK) cells, dendritic cells, monocytes, and T cells that have been stimulated by cellular ...
more infohttp://pubmedcentralcanada.ca/pmcc/articles/PMC2941891/

Expression and secretion of vascular endothelial growth factor-A by cytokine-stimulated hematopoietic progenitor cells:...Expression and secretion of vascular endothelial growth factor-A by cytokine-stimulated hematopoietic progenitor cells:...

In addition, we show that VEGF induced the release of hematopoietic growth factors (GM-CSF) by bone marrow endothelial cells ... Expression and secretion of vascular endothelial growth factor-A by cytokine-stimulated hematopoietic progenitor cells: ... maintenance of progenitor and stem cells due to adhesive interactions and paracrine secretion of hematopoietic growth factors. ... and release of vascular endothelial growth factor-A (VEGF-A), which specifically acts on endothelial cells, by cytokine- ...
more infohttp://vivo.med.cornell.edu/display/pubid0034085455

Combination of hematopoietic growth factors containing IL-3 induce acute myeloid leukemia cell sensitization to cycle specific...Combination of hematopoietic growth factors containing IL-3 induce acute myeloid leukemia cell sensitization to cycle specific...

... cells. While clinical trials based on this growth factor/chemotherapy combination (GF/CT) are progressing with discordant ... Combination of hematopoietic growth factors containing IL-3 induce acute myeloid leukemia cell sensitization to cycle specific ... Laboratory studies have suggested that hematopoietic growth factors (GF), combined with cytosine-arabinoside (Ara-C) can ... We report that in AML clonogenic cell growth can be stimulated by cytokines in 50% of the samples (4/8), and that Ara-C ...
more infohttp://vivo.med.cornell.edu/display/pubid0028231057

How long after neutrophil recovery should myeloid growth factors be continued in autologous hematopoietic stem cell transplant...How long after neutrophil recovery should myeloid growth factors be continued in autologous hematopoietic stem cell transplant...

Since ANC often increases to very high levels with this strategy, we discontinued growth factor on the first day ANC reached ... How long after neutrophil recovery should myeloid growth factors be continued in autologous hematopoietic stem cell transplant ... How long after neutrophil recovery should myeloid growth factors be continued in autologous hematopoietic stem cell transplant ... How long after neutrophil recovery should myeloid growth factors be continued in autologous hematopoietic stem cell transplant ...
more infohttps://einstein.pure.elsevier.com/en/publications/how-long-after-neutrophil-recovery-should-myeloid-growth-factors--2

Systematic investigation of oxygen and growth factors in clinically valid ex vivo expansion of cord blood CD34+ hematopoietic...Systematic investigation of oxygen and growth factors in clinically valid ex vivo expansion of cord blood CD34+ hematopoietic...

... of growth factor combinations across multiple oxygen levels on the ex vivo expansion of cord blood CD34 hematopoietic cells ... growth factors. hematopoiesis. hematopoietic progenitor cells. oxygen. stem cell transplantation Summary Background aims. Cord ... of oxygen and growth factors in clinically valid ex vivo expansion of cord blood CD34+ hematopoietic progenitor cells. Tursky, ... of oxygen and growth factors in clinically valid ex vivo expansion of cord blood CD34+ hematopoietic progenitor cells, ...
more infohttp://dro.deakin.edu.au/view/DU:30050497

Hematopoietic growth factor production by cultured cells of human nasal polyp epithelial scrapings: kinetics, cell source, and...Hematopoietic growth factor production by cultured cells of human nasal polyp epithelial scrapings: kinetics, cell source, and...

These studies confirm an epithelial cell origin of BMC and EO growth and differentiation factors derived from nasal polyps and ... Hematopoietic growth factor production by cultured cells of human nasal polyp epithelial scrapings: kinetics, cell source, and ... Growth kinetics of nasal polyp epithelial cells (NPECs) were determined, and CSA in NPEC conditioned medium (CM) was assessed ... The conditions and cell sources for colony stimulating activity (CSA) production by nasal polyp epithelial scrapings were ...
more infohttps://read.qxmd.com/read/2659645/hematopoietic-growth-factor-production-by-cultured-cells-of-human-nasal-polyp-epithelial-scrapings-kinetics-cell-source-and-relationship-to-clinical-status

Reya Lab |   Identification of Adiponectin As a Novel Hematopoietic Stem Cell Growth FactorReya Lab | Identification of Adiponectin As a Novel Hematopoietic Stem Cell Growth Factor

Given the emergence of a growing number of adipokines, it is possible that these factors may also play a role in regulating ... Identification of Adiponectin As a Novel Hematopoietic Stem Cell Growth Factor 2007. /0 Comments/in Publications /by awp-admin ... Identification of Adiponectin As a Novel Hematopoietic Stem Cell Growth Factor.. DiMascio LN, Voermans C, Uqoezwa M, Duncan AW ... 21Identification of Adiponectin As a Novel Hematopoietic Stem Cell Growth Factor. ...
more infohttps://reya-lab.org/identification-of-adiponectin-as-a-novel-hematopoietic-stem-cell-growth-factor/

온라인 Ebook 다운로드 Blood Cell Biochemistry: Hematopoietic Cell Growth Factors and Their Receptors PDF온라인 Ebook 다운로드 Blood Cell Biochemistry: Hematopoietic Cell Growth Factors and Their Receptors PDF

... the field of hematopoietic growth factor research began with the work of Carnot... ... Hematopoietic Cell Growth Factors and Their Receptors PDF - Anne-Marie OFarrell, Taisei Kinoshita, Atsushi Miyajima (auth.), ... Hematopoietic Cell Growth Factors and Their Receptors 으니다. *다음 후 즉시다운로드,그전자책 Blood Cell Biochemistry: Hematopoietic Cell Growth ... Hematopoietic Cell Growth Factors and Their Receptors 브컴팩트한 약간living니다. get Blood Cell Biochemistry: Hematopoietic Cell Growth ...
more infohttp://perfectdating.eu/133007-how-to-download-e-books-blood-cell-biochemistry.php

Transforming growth factor-β1 functional polymorphisms in myeloablative sibling hematopoietic stem cell transplantation.  -...Transforming growth factor-β1 functional polymorphisms in myeloablative sibling hematopoietic stem cell transplantation. -...

Numerous genetic factors have a role in transplant outcome. Several functional polymorphisms have been identified in TGF-β1 ... Transforming growth factor-β1 functional polymorphisms in myeloablative sibling hematopoietic stem cell transplantation.. Berro ... Hematopoietic stem cell transplantation (HSCT) with sibling donors (s.d.) is a life-saving intervention for patients with ... Hematopoietic Stem Cell Transplantation/methods*. *Hematopoietic Stem Cell Transplantation/mortality. *Hematopoietic Stem Cell ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=145289

Infection, neutrophils, and hematopoietic growth factors in the pathogenesis of neonatal chronic lung disease.Infection, neutrophils, and hematopoietic growth factors in the pathogenesis of neonatal chronic lung disease.

... has persuaded investigators that inflammatory cells and mediators are key factors in the pathophysiology of the disease. High ... The role of granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor is stressed as a pivotal ... Hematopoietic Cell Growth Factors / physiology*. Humans. Infant, Newborn. Infection / complications*. Lung Diseases / etiology ... has persuaded investigators that inflammatory cells and mediators are key factors in the pathophysiology of the disease. High ...
more infohttp://www.biomedsearch.com/nih/Infection-neutrophils-hematopoietic-growth-factors/10986637.html

Institute of Cancer Research Repository -  Transforming growth factor-ss 1 polymorphisms and the outcome of hematopoietic stem...Institute of Cancer Research Repository - Transforming growth factor-ss 1 polymorphisms and the outcome of hematopoietic stem...

Transforming growth factor-ss 1 polymorphisms and the outcome of hematopoietic stem cell transplantation ... Apart from clinical factors well known to affect the outcome of HSCT, patient and donor genetics have been shown to play an ... Transforming growth factor-ss 1 polymorphisms and the outcome of hematopoietic stem cell transplantation. INTERNATIONAL JOURNAL ... versus-host-disease cytokine gene polymorphisms bone-marrow transplant regulatory t-cells tgf-beta growth-factor-beta-1 gene ...
more infohttp://publications.icr.ac.uk/11671/

Activation of hematopoietic growth factor signal transduction pathways by the human oncogene BCR/ABL.  - PubMed - NCBIActivation of hematopoietic growth factor signal transduction pathways by the human oncogene BCR/ABL. - PubMed - NCBI

Cytokine Growth Factor Rev. 1997 Mar;8(1):63-79. Research Support, Non-U.S. Govt; Research Support, U.S. Govt, P.H.S.; Review ... the cells are dependent on hematopoietic growth factors. On a molecular level, the BCR/ABL translocation is well characterized ... and transformation of hematopoietic growth factor-dependent cell lines leads to growth factor independence. In this review, we ... Cytokine Growth Factor Rev. 1997 Mar;8(1):63-79.. Activation of hematopoietic growth factor signal transduction pathways by the ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/9174663?dopt=Abstract

Differentiation and functional activity of human eosinophilic cells from an eosinophil HL-60 subline: response to recombinant...Differentiation and functional activity of human eosinophilic cells from an eosinophil HL-60 subline: response to recombinant...

... macrophage colony-stimulating factor [GM-CSF], granulocyte [G]-CSF, interleukin ( ... We studied the effect of hematopoietic growth factors (granulocyte- ... We studied the effect of hematopoietic growth factors (granulocyte- macrophage colony-stimulating factor [GM-CSF], granulocyte ... response to recombinant hematopoietic growth factors [published erratum appears in Blood 1992 Dec 1;80(11):2952] I Fabian I ...
more infohttps://ashpublications.org/blood/article/80/3/788/169260/Differentiation-and-functional-activity-of-human

Hematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice | PNASHematopoietic cells differentiate into both microglia and macroglia in the brains of adult mice | PNAS

... and stem cell factor (100 ng/ml). Growth factors were used to maintain early hematopoietic cells in cycle (13). All were ... Besides the cells of the vasculature, the brain comprises two general cell types: neurons and glial cells. Glial cells provide ... with the addition of stem cell factor to optimize transduction of reconstituting hematopoietic stem cells (13). C57BL/6J mice ( ... After 48 h in culture with growth factors, marrow cells were collected and added to tissue culture dishes containing the F5B ...
more infohttps://www.pnas.org/content/94/8/4080?ijkey=3283e481ecacfcfb775ddaf393b6b19f837a4483&keytype2=tf_ipsecsha

The Soluble Notch Ligand, Jagged-1, Inhibits Proliferation of CD34+ Macrophage Progenitors | SpringerLinkThe Soluble Notch Ligand, Jagged-1, Inhibits Proliferation of CD34+ Macrophage Progenitors | SpringerLink

Accelerated cell-cycling of hematopoietic progenitor cells by growth factors.Blood. 1995;86:73-79.PubMedGoogle Scholar ... Accelerated cell-cycling of hematopoietic progenitors by theflt3 ligand that is modulated by transforming growth factor-β.Blood ... The Notch ligand Jagged-1 represents a novel growth factor of human hematopoietic stem cells.J Exp Med. 2000;192:1365-1372. ... Transforming growth factor β selectively inhibits normal and leukemic human bone marrow cell growth in vitro.Blood. 1988;72: ...
more infohttps://link.springer.com/article/10.1007/BF02982040

Hematopoietic growth factor | biology | Britannica.comHematopoietic growth factor | biology | Britannica.com

Hematopoietic growth factors: The hematopoietic growth factors are potent regulators of blood cell proliferation and ... Other articles where Hematopoietic growth factor is discussed: therapeutics: ... In therapeutics: Hematopoietic growth factors. The hematopoietic growth factors are potent regulators of blood cell ...
more infohttps://www.britannica.com/science/hematopoietic-growth-factor

β-Catenin-Independent Activation of TCF1/LEF1 in Human Hematopoietic Tumor Cells through Interaction with ATF2 Transcription...β-Catenin-Independent Activation of TCF1/LEF1 in Human Hematopoietic Tumor Cells through Interaction with ATF2 Transcription...

ATF2 transcription factors cooperate with TCF1/LEF1 to promote the growth of hematopoietic tumor cells. We used a candidate ... GradlD, KönigA, WedlichD (2002) Functional diversity of Xenopus lymphoid enhancer factor/T-cell factor transcription factors ... Growth of hematopoietic tumor cells in soft agarose was determined by seeding 2×103 cells per 60-mm dish in 0.5% sea plaque ... 6. ATF2 and ATF7 knockdown in Ramos cells decreases TCF/LEF activity, Axin 2 expression and cell growth. (A-C) Ramos cells ...
more infohttps://www.prolekare.cz/casopisy/plos-genetics/2013-8/b-catenin-independent-activation-of-tcf1-lef1-in-human-hematopoietic-tumor-cells-through-interaction-with-atf2-transcription-factors-43311

Genetics Institute Inc. | SBIR.govGenetics Institute Inc. | SBIR.gov

THE OBJECTIVE OF THE PROJECT IS TO ESTABLISH HUMAN HEMATO- POIETIC PROGENITOR GROWTH FACTOR-DEPENDENT CELL LINES IN CULTURE.. ... ATTACHMENT TO SOLUBLE POLYMERS ARE TO BE TESTED IN DETAIL TO ISOLATE THE EFFECT OF MULTI-POINT ATTACHMENT FROM OTHER FACTORS ... THE OBJECTIVE OF THE PROJECT IS TO ESTABLISH HUMAN HEMATO- POIETIC PROGENITOR GROWTH FACTOR-DEPENDENT CELL LINES IN CULTURE. TO ... GENETIC ENGINEERING OF AN IMPROVED FACTOR VIII PRODUCT. Amount: $500,000.00 FACTOR VIII IS THE PLASMA PROTEIN THAT IS ...
more infohttps://www.sbir.gov/sbirsearch/detail/173726

In vitro trans-differentiation of human umbilical cord derived hematopoietic stem cells into hepatocyte like cells using...In vitro trans-differentiation of human umbilical cord derived hematopoietic stem cells into hepatocyte like cells using...

... derived from UCB into hepatocyte like cells and also to estimate the effects of combination... ... The aim of the study was to develop a new strategy for the differentiation of hematopoietic stem cell (HSC) ... human umbilical cord derived hematopoietic stem cells into hepatocyte like cells using combination of growth factors for cell ... and hepatocyte growth factor (HGF) on hematopoietic stem cell differentiation. HSCs were isolated and purified by magnetic ...
more infohttps://link.springer.com/article/10.1007/s10616-011-9337-x

Granulosa-Theca Cell Tumors Follow-up: Further Outpatient Care, Inpatient & Outpatient Medications, TransferGranulosa-Theca Cell Tumors Follow-up: Further Outpatient Care, Inpatient & Outpatient Medications, Transfer

... with epithelial cell tumors (>70%) comprising the largest group of tumors. Germ cell tumors occur less frequently (20%), while ... Hematopoietic growth factors. A host of growth factors now can be given in place of antibiotics and transfusion of blood ... Available agents include granulocyte colony-stimulating factor (neutrophil-specific), granulocyte-macrophage colony-stimulating ... cell viability and apoptosis in ovarian granulosa cell tumor cells. PLoS One. 2014. 9(1):e85545. [Medline]. [Full Text]. ...
more infohttps://emedicine.medscape.com/article/254489-followup
  • Continuous VEGF infusion, at rates as low as 50 ng/h (resulting in serum VEGF concentrations of 120 to 160 pg/mL), resulted in a dramatic inhibition of dendritic cell development, associated with an increase in the production of B cells and immature Gr-1 + myeloid cells. (bloodjournal.org)
  • Pegfilgrastim is a long-acting filgrastim created by the covalent conjugate of recombinant granulocyte colony-stimulating factor (ie, filgrastim) and monomethoxypolyethylene glycol. (medscape.com)
  • We ask in this study whether recombinant VEGF alone at relevant concentrations would cause significant alterations in DC development in vivo in otherwise healthy animals, which cell populations would be most affected, and what role other factors may play in the effects mediated by VEGF. (bloodjournal.org)
  • Unlike TNF and Fas ligand (FasL), which can cause life-threatening toxic effects when used systemically ( 7 , 8 ), recombinant TRAIL protein has been shown to induce cell death in many types of tumor and transformed cells, but not in normal cells, in culture and xenograft animal models ( 5 , 6 , 9 , 10 ). (pubmedcentralcanada.ca)
  • In addition, initial xenograft studies using recombinant human soluble forms of the TRAIL protein showed TRAIL-induced apoptosis of human cancer cells transplanted into immunodeficient mice without toxicity to the normal tissues of the mice bearing these xenograft tumors ( 9 , 10 ). (pubmedcentralcanada.ca)
  • Upon ligand-mediated activation, Wnt receptors promote the stabilization of β-catenin, which translocates to the nucleus and binds to the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcription factors to regulate the expression of Wnt target genes. (prolekare.cz)
  • Ligands that binds with receptors on the cell-membrane of immune systems to trigger reactions, signal transductions. (wikibooks.org)
  • This agent is a purified glycoprotein produced from mammalian cells modified with gene coding for human erythropoietin (EPO). (medscape.com)
  • F5B cells shed the N2 retroviral vector, packaged with the ecotropic envelope and carrying the bacterial gene for neomycin resistance (neo R ) ( 14 ). (pnas.org)
  • Fiegel HC, Lioznov MV (2003) Cortes-Dericks liver-specific gene expression in cultured human hematopoietic stem cells. (springer.com)
  • Presented in part at the Keystone Symposium "Cellular and Molecular Biology of Dendritic Cells," Santa Fe, NM, March 3-9, 1998, and at the annual meeting of the American Association for Cancer Research, March 28-April 1, 1998. (bloodjournal.org)
  • Similarly, NPEC CM from allergic and nonallergic donors had equivalent CSA for basophil/mast cell (BMC) and eosinophil (EO) lineages, respectively. (qxmd.com)
  • GM-CSF-, IL-3-, and IL-5-treated Eos-HL-60 cells showed increased O2- production in response to phorbol esters (PMA), enhanced phagocytosis of Candida albicans, and release of the enzymes arylsulfatase, beta-glucuronidase and eosinophil peroxidase (EPO). (ashpublications.org)
  • Di Campli C, Piscaglia AC, Pierelli L et al (2004) A human umbilical cord stem cell rescue therapy in a murine model of toxic liver injury. (springer.com)
  • Feng G, De-Quan W, Yah-Hua H et al (2008) Extracellular matrix gel is necessary for in vitro cultivation of insulin producing cells from human umbilical cord blood derived mesenchymal stem cells. (springer.com)
  • Ishikawa F, Drake CJ, Yang S et al (2003) Transplanted human cord blood cells give rise to hepatocytes in engrafted mice. (springer.com)
  • Apart from clinical factors well known to affect the outcome of HSCT, patient and donor genetics have been shown to play an important role in the susceptibility to post-transplant complications. (icr.ac.uk)
  • One of the genes that may play an important role on the outcome of allogeneic HSCT is TGFB1, which encodes transforming growth factor, beta eta 1 (TGF-beta 1). (icr.ac.uk)
  • Ofatumumab is an anti-CD20 human monoclonal antibody that inhibits B-cell activation in early stages. (medscape.com)
  • In immune system, the signal for cells leads to activation of different cell specific immune activities. (wikibooks.org)
  • The prognosis for granulosa-theca cell tumors generally is very favorable. (medscape.com)
  • Growth factors are routinely used after autotransplantation to accelerate hematopoietic recovery, and are continued until the absolute neutrophil count (ANC) is ≥0.5 × 10 9 /l on 3 consecutive days. (elsevier.com)
  • We conclude that growth factors can be discontinued after autotransplantation the day the ANC reaches 0.5 × 10 9 /l, without compromising neutrophil recovery. (elsevier.com)
  • This article reviews the mechanisms underlying neutrophil recruitment in the lungs of ventilated preterm infants with respiratory distress syndrome and the injurious effects that these cells can produce on lung parenchyma with special emphasis on the development of CLD. (biomedsearch.com)
  • Plouet, J., Schilling, J. & Gospodarowicz, D. Isolation and characterization of a newly identified endothelial cell mitogen produced by AtT20 cells. (nature.com)
  • To test the ability of hematopoietic cells to contribute to the central nervous system, we have transplanted adult female mice with donor bone marrow cells genetically marked either with a retroviral tag or by using male donor cells. (pnas.org)
  • Marrow-derived cells were already detected in the brains of mice 3 days after transplant, and their numbers increased over the next several weeks, exceeding 14,000 cells per brain in several animals. (pnas.org)
  • When in situ hybridization histochemistry was combined with immunohistochemical staining using lineage-specific markers, some bone marrow-derived cells were positive for the microglial antigenic marker F4/80. (pnas.org)
  • Other marrow-derived cells surprisingly expressed the astroglial marker glial fibrillary acidic protein. (pnas.org)
  • We combined ISHH and immunohistochemistry, and performed double-ISHH with digoxigenin and radioactively labeled probes to analyze which cell types might be derived from bone marrow stem cells. (pnas.org)
  • After 48 h in culture with growth factors, marrow cells were collected and added to tissue culture dishes containing the F5B producer cell line at subconfluent density. (pnas.org)
  • After 48 h coculture with F5B cells, bone marrow cells were collected by gentle aspiration, suspended to 1 × 10 7 cells per ml in PBS (in all cases 0.1 M phosphate/140 mM NaCl, pH 7.6) and injected intravenously (2-3 × 10 6 cells per mouse) via the tail vein into sublethally irradiated (4.5 Gy) female WBB6F1/J- Kit W /Kit W-v mice. (pnas.org)
  • TCF1 and LEF1 physically interacted and functionally synergized with members of the activating transcription factor 2 (ATF2) family of transcription factors. (prolekare.cz)
  • Delayed-addition experiments and the analysis of colony sizes demonstrated that soluble hJagged-1 inhibited the growth of macrophage progenitors by acting in the early stage of macrophage development. (springer.com)
  • This study has identified optimized fold expansion conditions, with the potential for direct clinical translation to increase transplantable cell dose and as a baseline methodology against which future factors can be tested. (edu.au)
  • The conditions and cell sources for colony stimulating activity (CSA) production by nasal polyp epithelial scrapings were examined. (qxmd.com)
  • Given the emergence of a growing number of adipokines, it is possible that these factors may also play a role in regulating hematopoiesis. (reya-lab.org)
  • Milner LA, Bigas A. Notch as a mediator of cell fate determination in hematopoiesis: evidence and speculation. (springer.com)
  • There were comparable metachromatic cell counts in polyp epithelial scrappings from allergic and nonallergic donors. (qxmd.com)