AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceNamed GroupsHealth Care
GranulocytesGranulocyte Colony-Stimulating FactorReceptors, Granulocyte Colony-Stimulating FactorGranulocyte-Macrophage Colony-Stimulating FactorNeutrophilsLeukocytesHematopoietic Stem CellsLeukocyte CountColony-Stimulating FactorsHematopoiesisAgranulocytosisBone Marrow CellsNeutropeniaRecombinant ProteinsBone MarrowHematopoietic Stem Cell MobilizationInterleukin-3Colony-Forming Units AssayPhagocytosisMonocytesCell DifferentiationCells, CulturedLeukemia, MyeloidRespiratory BurstAntigens, CD34Leukocyte TransfusionN-Formylmethionine Leucyl-PhenylalanineMacrophagesEosinophilsChemotaxis, LeukocyteAntigens, CDLeukocytosisMyelopoiesisBlood CellsMice, Inbred C57BLPeroxidaseStem Cell FactorMyeloid Progenitor CellsBlood Bactericidal ActivityCytokinesGranulocyte Precursor CellsLymphocytesIndiumLeukopoiesisReceptors, Granulocyte-Macrophage Colony-Stimulating FactorNitroblue TetrazoliumGrowth SubstancesCell DivisionCell SeparationCyclophosphamideNeutrophil ActivationTime FactorsLeukopeniaHematopoietic Cell Growth FactorsLeukemia, Myeloid, AcuteZymosanHL-60 CellsHematopoietic Stem Cell TransplantationCell LineMacrophage Colony-Stimulating FactorAntibodies, MonoclonalChemokine CXCL6Myeloid CellsBlood Component RemovalRNA, MessengerLactoferrinGranulomatous Disease, ChronicMice, Inbred BALB CBone Marrow TransplantationHemoglobinuria, ParoxysmalSpleenMacrophage-1 AntigenAntineoplastic Combined Chemotherapy ProtocolsChemotactic FactorsAnemia, AplasticSuperoxidesAntigens, CD15Cell AdhesionInterleukin-6LeukemiaDose-Response Relationship, DrugCell LineageGranulocyte-Macrophage Progenitor CellsLeukotriene B4Platelet CountTetradecanoylphorbol AcetateCytapheresisCell CountOpsonin ProteinsLuminescent MeasurementsReceptors, Colony-Stimulating FactorDrug SynergismTumor Necrosis Factor-alphaInflammationCytoplasmic GranulesT-LymphocytesKineticsTransplantation, AutologousLeukemoid ReactionComplement C5a
Granulocytes
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
Granulocyte Colony-Stimulating Factor
A glycoprotein of MW 25 kDa containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines.
Receptors, Granulocyte Colony-Stimulating Factor
Receptors that bind and internalize GRANULOCYTE COLONY-STIMULATING FACTOR. Their MW is believed to be 150 kD. These receptors are found mainly on a subset of myelomonocytic cells.
Granulocyte-Macrophage Colony-Stimulating Factor
An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
Neutrophils
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Leukocytes
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
Hematopoietic Stem Cells
Progenitor cells from which all blood cells derive.
Leukocyte Count
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Colony-Stimulating Factors
Glycoproteins found in a subfraction of normal mammalian plasma and urine. They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. The factors include INTERLEUKIN-3; (IL-3); GRANULOCYTE COLONY-STIMULATING FACTOR; (G-CSF); MACROPHAGE COLONY-STIMULATING FACTOR; (M-CSF); and GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; (GM-CSF).
Hematopoiesis
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
Agranulocytosis
A decrease in the number of GRANULOCYTES; (BASOPHILS; EOSINOPHILS; and NEUTROPHILS).
Bone Marrow Cells
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Neutropenia
A decrease in the number of NEUTROPHILS found in the blood.
Recombinant Proteins
Proteins prepared by recombinant DNA technology.
Bone Marrow
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Hematopoietic Stem Cell Mobilization
The release of stem cells from the bone marrow into the peripheral blood circulation for the purpose of leukapheresis, prior to stem cell transplantation. Hematopoietic growth factors or chemotherapeutic agents often are used to stimulate the mobilization.
Interleukin-3
A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.
Colony-Forming Units Assay
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Phagocytosis
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
Monocytes
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Cell Differentiation
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Leukemia, Myeloid
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Respiratory Burst
A large increase in oxygen uptake by neutrophils and most types of tissue macrophages through activation of an NADPH-cytochrome b-dependent oxidase that reduces oxygen to a superoxide. Individuals with an inherited defect in which the oxidase that reduces oxygen to superoxide is decreased or absent (GRANULOMATOUS DISEASE, CHRONIC) often die as a result of recurrent bacterial infections.
Antigens, CD34
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Leukocyte Transfusion
The transfer of leukocytes from a donor to a recipient or reinfusion to the donor.
N-Formylmethionine Leucyl-Phenylalanine
A formylated tripeptide originally isolated from bacterial filtrates that is positively chemotactic to polymorphonuclear leucocytes, and causes them to release lysosomal enzymes and become metabolically activated.
Macrophages
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Eosinophils
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Chemotaxis, Leukocyte
The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.
Antigens, CD
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Leukocytosis
A transient increase in the number of leukocytes in a body fluid.
Myelopoiesis
Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.
Blood Cells
The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.
Mice, Inbred C57BL
Peroxidase
A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.
Stem Cell Factor
A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.
Myeloid Progenitor Cells
Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.
Blood Bactericidal Activity
The natural bactericidal property of BLOOD due to normally occurring antibacterial substances such as beta lysin, leukin, etc. This activity needs to be distinguished from the bactericidal activity contained in a patient's serum as a result of antimicrobial therapy, which is measured by a SERUM BACTERICIDAL TEST.
Cytokines
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Granulocyte Precursor Cells
The cells in the granulocytic series that give rise to mature granulocytes (NEUTROPHILS; EOSINOPHILS; and BASOPHILS). These precursor cells include myeloblasts, promyelocytes, myelocytes and metamyelocytes.
Lymphocytes
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Indium
A metallic element, atomic number 49, atomic weight 114.82, symbol In. It is named from its blue line in the spectrum. (From Dorland, 28th ed)
Leukopoiesis
The process of generating white blood cells (LEUKOCYTES) from the pluripotent HEMATOPOIETIC STEM CELLS of the BONE MARROW. There are two significant pathways to generate various types of leukocytes: MYELOPOIESIS, in which leukocytes in the blood are derived from MYELOID STEM CELLS, and LYMPHOPOIESIS, in which leukocytes of the lymphatic system (LYMPHOCYTES) are generated from lymphoid stem cells.
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Receptors that bind and internalize the granulocyte-macrophage stimulating factor. Their MW is believed to be 84 kD. The most mature myelomonocytic cells, specifically human neutrophils, macrophages, and eosinophils, express the highest number of affinity receptors for this growth factor.
Nitroblue Tetrazolium
Colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.
Growth Substances
Signal molecules that are involved in the control of cell growth and differentiation.
Cell Division
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Cell Separation
Cyclophosphamide
Precursor of an alkylating nitrogen mustard antineoplastic and immunosuppressive agent that must be activated in the LIVER to form the active aldophosphamide. It has been used in the treatment of LYMPHOMA and LEUKEMIA. Its side effect, ALOPECIA, has been used for defleecing sheep. Cyclophosphamide may also cause sterility, birth defects, mutations, and cancer.
Neutrophil Activation
The process in which the neutrophil is stimulated by diverse substances, resulting in degranulation and/or generation of reactive oxygen products, and culminating in the destruction of invading pathogens. The stimulatory substances, including opsonized particles, immune complexes, and chemotactic factors, bind to specific cell-surface receptors on the neutrophil.
Time Factors
Elements of limited time intervals, contributing to particular results or situations.
Leukopenia
Hematopoietic Cell Growth Factors
These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.
Leukemia, Myeloid, Acute
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Zymosan
HL-60 Cells
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Hematopoietic Stem Cell Transplantation
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Macrophage Colony-Stimulating Factor
A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. M-CSF is a disulfide-bonded glycoprotein dimer with a MW of 70 kDa. It binds to a specific high affinity receptor (RECEPTOR, MACROPHAGE COLONY-STIMULATING FACTOR).
Antibodies, Monoclonal
Antibodies produced by a single clone of cells.
Chemokine CXCL6
A CXC chemokine that has stimulatory and chemotactic activities towards NEUTROPHILS. It has specificity for CXCR1 RECEPTORS and CXCR2 RECEPTORS.
Myeloid Cells
The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).
Blood Component Removal
Any procedure in which blood is withdrawn from a donor, a portion is separated and retained and the remainder is returned to the donor.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Lactoferrin
An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion.
Granulomatous Disease, Chronic
A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.
Mice, Inbred BALB C
Bone Marrow Transplantation
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
Hemoglobinuria, Paroxysmal
A condition characterized by the recurrence of HEMOGLOBINURIA caused by intravascular HEMOLYSIS. In cases occurring upon cold exposure (paroxysmal cold hemoglobinuria), usually after infections, there is a circulating antibody which is also a cold hemolysin. In cases occurring during or after sleep (paroxysmal nocturnal hemoglobinuria), the clonal hematopoietic stem cells exhibit a global deficiency of cell membrane proteins.
Spleen
An encapsulated lymphatic organ through which venous blood filters.
Macrophage-1 Antigen
An adhesion-promoting leukocyte surface membrane heterodimer. The alpha subunit consists of the CD11b ANTIGEN and the beta subunit the CD18 ANTIGEN. The antigen, which is an integrin, functions both as a receptor for complement 3 and in cell-cell and cell-substrate adhesive interactions.
Antineoplastic Combined Chemotherapy Protocols
The use of two or more chemicals simultaneously or sequentially in the drug therapy of neoplasms. The drugs need not be in the same dosage form.
Chemotactic Factors
Chemical substances that attract or repel cells. The concept denotes especially those factors released as a result of tissue injury, microbial invasion, or immunologic activity, that attract LEUKOCYTES; MACROPHAGES; or other cells to the site of infection or insult.
Anemia, Aplastic
A form of anemia in which the bone marrow fails to produce adequate numbers of peripheral blood elements.
Superoxides
Highly reactive compounds produced when oxygen is reduced by a single electron. In biological systems, they may be generated during the normal catalytic function of a number of enzymes and during the oxidation of hemoglobin to METHEMOGLOBIN. In living organisms, SUPEROXIDE DISMUTASE protects the cell from the deleterious effects of superoxides.
Antigens, CD15
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Cell Adhesion
Adherence of cells to surfaces or to other cells.
Interleukin-6
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Leukemia
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
Dose-Response Relationship, Drug
The relationship between the dose of an administered drug and the response of the organism to the drug.
Cell Lineage
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
Granulocyte-Macrophage Progenitor Cells
The parent cells that give rise to both cells of the GRANULOCYTE lineage and cells of the monocyte/macrophage lineage.
Leukotriene B4
The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)
Platelet Count
The number of PLATELETS per unit volume in a sample of venous BLOOD.
Tetradecanoylphorbol Acetate
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Cytapheresis
Separation of one or more kinds of cells from whole blood with the return of other blood cell constituents to the patient or donor. This is accomplished with an instrument that uses centrifugation to separate the cells into different layers based on the differences in cell density (displacement) or drag coefficients in a current (elutriation). The procedure is commonly used in adoptive transfer to isolate NK cells, lymphocytes, or monocytes.
Cell Count
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Opsonin Proteins
Proteins that bind to particles and cells to increase susceptibility to PHAGOCYTOSIS, especially ANTIBODIES bound to EPITOPES that attach to FC RECEPTORS. COMPLEMENT C3B may also participate.
Luminescent Measurements
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Receptors, Colony-Stimulating Factor
Cell surface receptors for colony stimulating factors, local mediators, and hormones that regulate the survival, proliferation, and differentiation of hemopoietic cells.
Drug Synergism
The action of a drug in promoting or enhancing the effectiveness of another drug.
Tumor Necrosis Factor-alpha
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Inflammation
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Cytoplasmic Granules
Condensed areas of cellular material that may be bounded by a membrane.
T-Lymphocytes
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Kinetics
The rate dynamics in chemical or physical systems.
Transplantation, Autologous
Transplantation of an individual's own tissue from one site to another site.
Leukemoid Reaction
A peripheral blood picture resembling that of leukemia or indistinguishable from it on the basis of morphologic appearance alone. (Dorland, 27th ed)
Complement C5a
The minor fragment formed when C5 convertase cleaves C5 into C5a and COMPLEMENT C5B. C5a is a 74-amino-acid glycopeptide with a carboxy-terminal ARGININE that is crucial for its spasmogenic activity. Of all the complement-derived anaphylatoxins, C5a is the most potent in mediating immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE), smooth MUSCLE CONTRACTION; HISTAMINE RELEASE; and migration of LEUKOCYTES to site of INFLAMMATION.
Myelodysplastic Syndromes
Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.
Interleukin-8
A member of the CXC chemokine family that plays a role in the regulation of the acute inflammatory response. It is secreted by variety of cell types and induces CHEMOTAXIS of NEUTROPHILS and other inflammatory cells.
Pancreatic Elastase
A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC 3.4.21.36.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Plasmapheresis
Procedure whereby plasma is separated and extracted from anticoagulated whole blood and the red cells retransfused to the donor. Plasmapheresis is also employed for therapeutic use.
Leukemia, Promyelocytic, Acute
An acute myeloid leukemia in which abnormal PROMYELOCYTES predominate. It is frequently associated with DISSEMINATED INTRAVASCULAR COAGULATION.
Injections, Subcutaneous
Forceful administration under the skin of liquid medication, nutrient, or other fluid through a hollow needle piercing the skin.
Phagocytes
Cells that can carry out the process of PHAGOCYTOSIS.
Whole-Body Irradiation
Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.
Phagocyte Bactericidal Dysfunction
Disorders in which phagocytic cells cannot kill ingested bacteria; characterized by frequent recurring infection with formulation of granulomas.
Receptors, Interleukin-3
High affinity receptors for INTERLEUKIN-3. They are found on early HEMATOPOIETIC PROGENITOR CELLS; progenitors of MYELOID CELLS; EOSINOPHILS; and BASOPHILS. Interleukin-3 receptors are formed by the dimerization of the INTERLEUKIN-3 RECEPTOR ALPHA SUBUNIT and the CYTOKINE RECEPTOR COMMON BETA SUBUNIT.
Cell Movement
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Immunophenotyping
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Lipopolysaccharides
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Gene Expression Regulation
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Complement C5
C5 plays a central role in both the classical and the alternative pathway of COMPLEMENT ACTIVATION. C5 is cleaved by C5 CONVERTASE into COMPLEMENT C5A and COMPLEMENT C5B. The smaller fragment C5a is an ANAPHYLATOXIN and mediator of inflammatory process. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the MEMBRANE ATTACK COMPLEX.
Myeloproliferative Disorders
Conditions which cause proliferation of hemopoietically active tissue or of tissue which has embryonic hemopoietic potential. They all involve dysregulation of multipotent MYELOID PROGENITOR CELLS, most often caused by a mutation in the JAK2 PROTEIN TYROSINE KINASE.
Blood Transfusion
The introduction of whole blood or blood component directly into the blood stream. (Dorland, 27th ed)
Tumor Cells, Cultured
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Antigens, Differentiation
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
Receptors, IgG
Specific molecular sites on the surface of various cells, including B-lymphocytes and macrophages, that combine with IMMUNOGLOBULIN Gs. Three subclasses exist: Fc gamma RI (the CD64 antigen, a low affinity receptor), Fc gamma RII (the CD32 antigen, a high affinity receptor), and Fc gamma RIII (the CD16 antigen, a low affinity receptor).
Etoposide
A semisynthetic derivative of PODOPHYLLOTOXIN that exhibits antitumor activity. Etoposide inhibits DNA synthesis by forming a complex with topoisomerase II and DNA. This complex induces breaks in double stranded DNA and prevents repair by topoisomerase II binding. Accumulated breaks in DNA prevent entry into the mitotic phase of cell division, and lead to cell death. Etoposide acts primarily in the G2 and S phases of the cell cycle.
Cell Survival
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Dimethyl Sulfoxide
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Interleukin-1
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Antigens, CD11b
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
Bone Marrow Diseases
Skin Window Technique
A technique to study CELL MIGRATION in the INFLAMMATION process or during immune reactions. After an area on the skin is abraded, the movement of cells in the area is followed via microscopic observation of the exudate through a coverslip or tissue culture chamber placed over the area.
Membrane Glycoproteins
Glycoproteins found on the membrane or surface of cells.
Clone Cells
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Receptors, Complement
Molecules on the surface of some B-lymphocytes and macrophages, that recognize and combine with the C3b, C3d, C1q, and C4b components of complement.
N-Formylmethionine
Effective in the initiation of protein synthesis. The initiating methionine residue enters the ribosome as N-formylmethionyl tRNA. This process occurs in Escherichia coli and other bacteria as well as in the mitochondria of eucaryotic cells.
Treatment Outcome
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
Dendritic Cells
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
Cell Adhesion Molecules
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Drug Administration Schedule
Time schedule for administration of a drug in order to achieve optimum effectiveness and convenience.
Tropolone
A seven-membered aromatic ring compound. It is structurally related to a number of naturally occurring antifungal compounds (ANTIFUNGAL AGENTS).
Chemotaxis
The movement of cells or organisms toward or away from a substance in response to its concentration gradient.
Erythropoietin
Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
Blood Donors
Blood Proteins
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
Megakaryocytes
Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.
Basophils
Granular leukocytes characterized by a relatively pale-staining, lobate nucleus and cytoplasm containing coarse dark-staining granules of variable size and stainable by basic dyes.
Antigens, Surface
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Polycythemia Vera
A myeloproliferative disorder of unknown etiology, characterized by abnormal proliferation of all hematopoietic bone marrow elements and an absolute increase in red cell mass and total blood volume, associated frequently with splenomegaly, leukocytosis, and thrombocythemia. Hematopoiesis is also reactive in extramedullary sites (liver and spleen). In time myelofibrosis occurs.
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Tretinoin
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
Interleukin-5
A cytokine that promotes differentiation and activation of EOSINOPHILS. It also triggers activated B-LYMPHOCYTES to differentiate into IMMUNOGLOBULIN-secreting cells.

Steroids and hematopoiesis. III. The response of granulocytic and erythroid colony-forming cells to steroids of different classes. (1/3501)

Selected androgenic and nonandrogenic steroids enhance in vitro granulocytic and erythroid colony formation by mouse marrow cells, but do so by influencing either different target cells or cells in different states of cell cycle. Etiocholanolone, a naturally occurring nonandrogenic testosterone metabolite, permits cells not in active cycle to respond to colony-stimulating factor or erythropoietin. Fluoxymesterone, a synthetic androgen, appears to enhance colony growth by increasing the responsiveness of target cells to tropic stimuli. The majority of cells responding to this androgen are in active DNA synthesis. Direct comparison, however, of etiocholanolone-dependent erythroid or granulocytic colony-forming cells demonstrates nonidentity of the target cells. Thus colony-forming units responding to different classes of steroids are in different states of cell cycle and are physically separable. The enhancement of the in vitro response of colony-forming cells to regulating hormones by steroids such as etiocholanolane suggests a mechanism by which such agents may be therapeutically effective in certain cases of marrow failure in man.  (+info)

Yops of Yersinia enterocolitica inhibit receptor-dependent superoxide anion production by human granulocytes. (2/3501)

The virulence plasmid-borne genes encoding Yersinia adhesin A (YadA) and several Yersinia secreted proteins (Yops) are involved in the inhibition of phagocytosis and killing of Yersinia enterocolitica by human granulocytes. One of these Yops, YopH, dephosphorylates multiple tyrosine-phosphorylated proteins in eukaryotic cells and is involved in the inhibition of phagocytosis of Y. enterocolitica by human granulocytes. We investigated whether antibody- and complement-opsonized plasmid-bearing (pYV+) Y. enterocolitica inhibits O2- production by human granulocytes in response to various stimuli and whether YopH is involved. Granulocytes were preincubated with mutant strains unable to express YadA or to secrete Yops or YopH. O2- production by granulocytes during stimulation was assessed by measuring the reduction of ferricytochrome c. PYV+ Y. enterocolitica inhibited O2- production by granulocytes incubated with opsonized Y. enterocolitica or N-formyl-Met-Leu-Phe (f-MLP). This inhibitory effect mediated by pYV did not affect receptor-independent O2- production by granulocytes in response to phorbol myristate acetate, indicating that NADPH activity remained unaffected after activation of protein kinase C. The inhibition of f-MLP-induced O2- production by granulocytes depends on the secretion of Yops and not on the expression of YadA. Insertional inactivation of the yopH gene abrogated the inhibition of phagocytosis of antibody- and complement-opsonized Y. enterocolitica by human granulocytes but not of the f-MLP-induced O2- production by granulocytes or tyrosine phosphorylation of granulocyte proteins. These findings suggest that the specific targets for YopH are not present in f-MLP receptor-linked signal transduction and that other Yop-mediated mechanisms are involved.  (+info)

MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member. (3/3501)

Terminal cell differentiation is correlated with the extensive sequestering of previously active genes into compact transcriptionally inert heterochromatin. In vertebrate blood cells, these changes can be traced to the accumulation of a developmentally regulated heterochromatin protein, MENT. Cryoelectron microscopy of chicken granulocyte chromatin, which is highly enriched with MENT, reveals exceptionally compact polynucleosomes, which maintain a level of higher order folding above that imposed by linker histones. The amino acid sequence of MENT reveals a close structural relationship with serpins, a large family of proteins known for their ability to undergo dramatic conformational transitions. Conservation of the "hinge region" consensus in MENT indicates that this ability is retained by the protein. MENT is distinguished from the other serpins by being a basic protein, containing several positively charged surface clusters, which are likely to be involved in ionic interactions with DNA. One of the positively charged domains bears a significant similarity to the chromatin binding region of nuclear lamina proteins and with the A.T-rich DNA-binding motif, which may account for the targeting of MENT to peripheral heterochromatin. MENT ectopically expressed in a mammalian cell line is transported into nuclei and is associated with intranuclear foci of condensed chromatin.  (+info)

Transduction of primitive human marrow and cord blood-derived hematopoietic progenitor cells with adeno-associated virus vectors. (4/3501)

We evaluated the capacity of adeno-associated virus (AAV) vectors to transduce primitive human myeloid progenitor cells derived from marrow and cord blood in long-term cultures and long-term culture-initiating cell (LTC-IC) assays. Single-colony analyses showed that AAV vectors transduced CD34(+) and CD34(+)38(-) clonogenic cells in long-term culture. Gene transfer was readily observed in LTC-ICs derived from 5-, 8-, and 10-week cultures. Recombinant AAV (rAAV) transduction was observed in every donor analyzed, although a wide range of gene transfer frequencies (5% to 100%) was noted. AAV transduction of LTC-ICs was stable, with week-8 and -10 LTC-ICs showing comparable or better transduction relative to week-5 LTC-ICs. Fluorescence in situ hybridization (FISH) analyses performed to determine the fate of AAV vectors in transduced cells showed that 9% to 28% of CD34(+) and CD34(+)38(-) cells showed stable vector integration as evidenced by chromosome-associated signals in metaphase spreads. Comparisons of interphase and metaphase FISH suggested that a fraction of cells also contained episomal vector at early time points after transduction. Despite the apparent loss of the episomal forms with continued culture, the number of metaphases containing integrated vector genomes remained stable long term. Transgene transcription and placental alkaline phosphatase (PLAP) expression was observed in CD34(+), CD34(+)38(-) LTC-ICs in the absence of selective pressure. These results suggest that primitive myeloid progenitors are amenable to genetic modification with AAV vectors.  (+info)

A novel spliced form of SH2-containing inositol phosphatase is expressed during myeloid development. (5/3501)

SH2-containing Inositol Phosphatase (SHIP) is a 145 kD protein expressed in hematopoietic cells. SHIP is phosphorylated on tyrosine after receptor binding by several cytokines and has a negative role in hematopoiesis. We cloned a murine complementary DNA (cDNA) sequence for an isoform of SHIP with an internal 183 nucleotide deletion, encoding a protein 61 amino acids shorter than 145 kD SHIP. This deletion eliminates potential SH3-domain binding regions and a potential binding site for the p85 subunit of Phosphatidylinositol 3-Kinase. Using polyclonal anti-SHIP antibodies, we and others have previously observed a 135 kD SHIP isoform that is coexpressed with 145 kD SHIP. Here, we used monoclonal antibodies raised against the region deleted in the spliced form to show that the product of the novel spliced SHIP cDNA is antigenically identical to the 135 kD SHIP isoform. Like 145 kD SHIP, 135 kD SHIP expression was induced on differentiation of bone marrow cells. After macrophage colony-stimulating factor (M-CSF) stimulation of FDC-P1(Fms) myeloid cells, both 145 and 135 kD SHIP forms were tyrosine phosphorylated and could be coimmunoprecipitated with antibodies to Shc and Grb2. However, experiments showed only a weak association of 135 kD SHIP with p85. A potentially analogous 135 kD SHIP species also appears in human differentiated leukocytes.  (+info)

The intracellular serpin proteinase inhibitor 6 is expressed in monocytes and granulocytes and is a potent inhibitor of the azurophilic granule protease, cathepsin G. (6/3501)

The monocyte and granulocyte azurophilic granule proteinases elastase, proteinase 3, and cathepsin G are implicated in acute and chronic diseases thought to result from an imbalance between the secreted proteinase(s) and circulating serpins such as alpha1-proteinase inhibitor and alpha1-antichymotrypsin. We show here that the intracellular serpin, proteinase inhibitor 6 (PI-6), is present in monocytes, granulocytes, and myelomonocytic cell lines. In extracts from these cells, PI-6 bound an endogenous membrane-associated serine proteinase to form an sodium dodecyl sulfate (SDS)-stable complex. Using antibodies to urokinase, elastase, proteinase 3, or cathepsin G, we demonstrated that the complex contains cathepsin G. Native cathepsin G and recombinant PI-6 formed an SDS-stable complex in vitro similar in size to that observed in the extracts. Further kinetic analysis demonstrated that cathepsin G and PI-6 rapidly form a tight 1:1 complex (ka = 6.8 +/- 0.2 x 10(6) mol/L-1s-1 at 17 degrees C; Ki = 9.2 +/- 0.04 x 10(-10) mol/L). We propose that PI-6 complements alpha1-proteinase inhibitor and alpha1-antichymotrypsin (which control extracellular proteolysis) by neutralizing cathepsin G that leaks into the cytoplasm of monocytes or granulocytes during biosynthesis or phagocytosis. Control of intracellular cathepsin G may be particularly important, because it has recently been shown to activate the proapoptotic proteinase, caspase-7.  (+info)

Mechanisms of acute inflammatory lung injury induced by abdominal sepsis. (7/3501)

Sequestration of neutrophils and release of histotoxic mediators are considered important for the development of pathologic alterations of the lung defined as adult respiratory distress syndrome. Mechanisms of inflammatory lung injury caused by abdominal sepsis were investigated using the colon ascendens stent peritonitis (CASP) model that closely mimics the human disease. In the CASP model, a continuous leakage of intraluminal bacteria into the peritoneal cavity is induced by implantation of a stent in the ascending colon, generating a septic focus. In contrast to the cecal ligation and puncture model of peritonitis, survival of mice following CASP surgery is dependent on IFN-gamma, but independent of tumor necrosis factor (TNF). Here we show that the systemic inflammation induced by CASP surgery results in a rapid and profound increase of lung vascular permeability that was associated with the activation and recruitment of neutrophils to the lung. Activation of circulating granulocytes was characterized by increased production of serine proteinases and reactive oxygen metabolites, as well as elevated expression of cell surface Mac-1. Expression of MIP-2, KC, MIP-1alpha and E-selectin mRNA in lung was strongly increased within 3 h following CASP surgery, whereas up-regulation of IP-10, MCP-1 and P-selectin was delayed. In contrast, induction of RANTES, LIX, ICAM-1 and VCAM-1 mRNA was weak or not detectable after CASP surgery. Importantly, recruitment of leukocytes to the lung was normal in lipopolysaccharide-resistant mice, and was not affected by antibody neutralization of TNF or the chemokines MIP-2 and KC.  (+info)

Bone marrow scintigraphy using technetium-99m antigranulocyte antibody in malignant lymphomas. (8/3501)

BACKGROUND: The purpose of this study was to elucidate the clinical reliability of immunoscintigraphy (IS) to detect infiltration of the bone marrow in patients with malignant lymphoma. PATIENTS AND METHODS: Whole body IS was performed in 103 patients with Hodgkin's disease (HD) or non-Hodgkin's lymphoma (NHL) using Tc-99m labelled anti-NCA-95 which allows visualization of the granulopoietic bone marrow. Of these, 52% were studied prior to any therapy. Findings were compared to posterior iliac crest biopsy as well as MRI and/or follow-up examination. Criteria of marrow infiltration were a positive biopsy, positive follow-up, or positive results of MRI. RESULTS: Comparison of IS and biospy revealed concordant findings in 69 and discordant findings in 34 of 103 patients. Of the 34 patients with discordant results, IS showed lesions suspicious of bone marrow infiltration in 29 patients despite normal biopsy findings. When follow-up and additional examinations were taken into consideration, 10 patients remained with probably false positive and five with false negative IS findings. IS proved to be highly sensitive and specific in patients with HD (100% and 84%, respectively) and high-grade NHL (93% and 84%, respectively). Moderate sensitivity (60%) was found in low-grade NHL. This was possibly due to false negative IS in three to five patients with chemotherapy in contrast to one of five false negative results in patients without chemotherapy. CONCLUSION: Bone marrow scintigraphy using antigranulocyte antibodies is highly sensitive in HD and high-grade NHL. Positive findings in IS subsequent to a negative biopsy should be followed by guided re-biopsy or MRI.  (+info)

Selective reduction of human tumor cell populations by human granulocytes in vitro<...Selective reduction of human tumor cell populations by human granulocytes in vitro<...
TY - JOUR. T1 - Selective reduction of human tumor cell populations by human granulocytes in vitro. AU - Townsend, Courtney. AU - Townsend, C. M.. AU - Galbraith, M. A.. AU - Eilber, F. R.. AU - Morton, D. L.. PY - 1978. Y1 - 1978. N2 - Human peripheral blood granulocytes at a 10:1 effector cell:target cell ratio were shown by an in vitro semiquantitative staining procedure to reduce the number of human tumor cells, but not that of normal cells. Microscopic observations revealed that this selective reduction of tumor cells by granulocytes was a function of both detachment and cytolysis. The cytotoxic effect of granulocytes on the tumor cells was confirmed by a quantitative 5-[ 125I]iodo-2-deoxyuridine release assay. The data indicate that human granulocytes at a relatively low effector:target cell ratio (10:1) have the capacity to recognize and destroy human tumor cells in vitro.. AB - Human peripheral blood granulocytes at a 10:1 effector cell:target cell ratio were shown by an in vitro ...
https://researchexperts.utmb.edu/en/publications/selective-reduction-of-human-tumor-cell-populations-by-human-gran
Granulocytes are the major type of phagocytes constituting the front line of innate immune defense against bacterial infection ...Granulocytes are the major type of phagocytes constituting the front line of innate immune defense against bacterial infection ...
Granulocytes are the major type of phagocytes constituting the front line of innate immune defense against bacterial infection. to treat serious infection in alcoholic patients with the disorder of granulopoieis. This article reviews the recent development in understanding alcohol-induced disruption of marrow granulopoiesis and the granulopoietic response with the focus on progress in delineating cell signaling mechanisms underlying the alcohol-induced injury to hematopoietic tissue. Attempts in exploring effective therapy to boost individual treatment Larotaxel with this field shall also end up being discussed. tradition of marrow cells from regular individuals in nutritional medium containing alcoholic beverages can induce cytoplasmic vacuolization (Yeung, et al., 1988). The essential alcoholic beverages focus for inducing vacuolization can be 62.5 mg/dl. The percentage of cells developing vacuoles shows up correlating using the focus of alcoholic beverages. In the center, vacuolization in ...
https://gbic2012.org/%EF%BB%BFgranulocytes-are-the-major-type-of-phagocytes-constituting-the-front-line-of-innate-immune-defense-against-bacterial-infection/
Development of Oxidase activity by human bone marrow granulocytes | Blood | American Society of HematologyDevelopment of Oxidase activity by human bone marrow granulocytes | Blood | American Society of Hematology
B Zakhireh, RK Root; Development of Oxidase activity by human bone marrow granulocytes, Blood, Volume 54, Issue 2, 1 August 1979, Pages 429-439, https://doi.org
https://ashpublications.org/blood/article/54/2/429/161530/Development-of-Oxidase-activity-by-human-bone
Dose in Granulocyte Transfusion | Annals of Internal Medicine | American College of PhysiciansDose in Granulocyte Transfusion | Annals of Internal Medicine | American College of Physicians
To the editor: In a recent article, Winston and associates (1) presented data that led them to conclude that therapeutic granulocyte transfusions offered no substantial benefit over optimal antimicrobial therapy alone in managing infected patients with granulocytopenia. We have difficulty extrapolating their observations to granulocyte transfusion therapy as given at our institution (2) and others (3) where doses of three to six times as many granulocytes are administered routinely. The authors have criticized other randomized trials of granulocyte transfusions in neutropenic patients for having too few patients. We find the major weakness of this study to be too few granulocytes ...
https://annals.org/aim/article-abstract/696275/dose-granulocyte-transfusion
and peripheral blood granulocytes. CD10 is a regulator of B cell growth and proliferation. CD10 is used in conjunction with...and peripheral blood granulocytes. CD10 is a regulator of B cell growth and proliferation. CD10 is used in conjunction with...
Background LINK-A lncRNA acts as an oncogene in triple-negative breast cancer, but its involvement in other diseases is unknown. levels of LINK-A lncRNA and survivin were positively correlated in mantle cell lymphoma patients however, not in healthful controls. Conclusions LINK-A overexpression marketed cell proliferation lncRNA, inhibited cell apoptosis, and upregulated survivin appearance, while LINK-A lncRNA knockdown got the opposite impact. cell proliferation assay After transfection, CCK-8 assay was performed to identify cell proliferation. Cells had been gathered and cell suspensions using a cell thickness of 6104 cells per ml had been ready. Each well of the 96-well dish was filled up with a 0.1-ml cell suspension containing 6103 cells and cells were cultured within an incubator (37C, 5% CO2). CCK-8 option (10 ul, Sigma-Aldrich) was added 24, 48, 72, and 96 h afterwards. After that, cells had been cultured for yet another 4 h and OD beliefs at 450 nM had been assessed utilizing a ...
https://aksumuniversity.org/tag/and-peripheral-blood-granulocytes-cd10-is-a-regulator-of-b-cell-growth-and-proliferation-cd10-is-used-in-conjunction-with-other-reagents-in-the-phenotyping-of-leukemia/
The Effects of Oxidizing Species Derived from Molecular Oxygen on the Proliferation In Vitro of Human Granulocyte‐Macrophage...
TY - JOUR. T1 - The Effects of Oxidizing Species Derived from Molecular Oxygen on the Proliferation In Vitro of Human Granulocyte‐Macrophage Progenitor Cells. AU - BROXMEYER, HAL E.. AU - COOPER, SCOTT. AU - GABIC, THEODORE. PY - 1989/5. Y1 - 1989/5. N2 - In order to better understand the enhancing effects of lowered oxygen (O2) tension on the growth in vitro of granulocyte-macrophage progenitor cells (CFU-GM), the effects of oxidizing species derived from molecular O2 were assessed on CFU-GM. Low density or nonadherent low density normal human bone marrow cells were plated at ambient (20%) or lowered (5%) O2 tension in the presence of a source of colony stimulating factors, and in the absence or presence of superoxide dismutase, catalase, glucose oxidase or horseradish peroxidase, alone or in various combinations. Enhanced colony and cluster formation of CFU-GM was noted when low density cells were grown at 5% O2, or when cells were grown at 20% O2 in the presence of superoxide dismutase or ...
https://indiana.pure.elsevier.com/en/publications/the-effects-of-oxidizing-species-derived-from-molecular-oxygen-on
CD11/CD18 Antibody Review
The anti-CD11/CD18 monoclonal antibodies (mAbs) submitted in the Second International Workshop on Ruminant Leukocyte Differentiation Antigens, were analysed for their reactivity with the ovine homologue of CD11/CD18. Their reactivity was tested on healthy sheep tissues, and alveolar macrophages, afferent dendritic cells, peripheral blood granulocytes and monocytes. The CD11a/CD18 mAbs found positive in the sheep were reactive with all the cell populations tested. The CD11b mAbs reacted with all the cells except afferent dendritic cells, whereas CD11c were non-reactive to blood granulocytes. This is in contrast to humans and cattle where blood granulocytes express CD11c. PMID: 8310662. ...
http://antibodybeyond.com/reviews/antigen-c/cd11.cd18.htm
Phoebe Rose Rocks: ProgressPhoebe Rose Rocks: Progress
We are at the hospital every day, sometimes two or three times a day if the day includes labs and doctors visits in addition to the daily two hour infusion of anti-fungal medicine. This medicine can cause a drop in potassium, so each morning we arrive to check Phoebes potassium and then return hours later for the medicine. We try to time this around meal times and quality time so that it doesnt feel like we are always at the hospital. The days that include infusions of granulocytes are especially busy and include an overnight stay at the hospital. The granulocytes need to be given 12 hours after the antifungal medicine, so on these days, Jon brings Phoebe in to the medicine room at 4 am and I arrive at the hospital at 8:30am to donate granulocytes. The donation process takes about two and a half hours and the granulocytes are started once we are admitted to the in patient unit in the early evening. Its busy. Right now we are giving granulocytes once a week on Fridays and Im hoping that I can ...
http://phoeberoserocks.blogspot.com/2015/05/progress.html
Genevisible - Top 10 tissues for 1552667 a at
Top 10 tissues for 1552667_a_at (Homo sapiens, Affymetrix Probeset): peripheral blood granulocyte, blood neutrophil granulocyte, granulocyte (polymorphonuclear leukocyte), double positive thymocyte, peripheral blood lymphocyte, splenic CD8 activated T-cell (unspecified), thymocyte, peripheral blood CD8 cytotoxic T-cell (unspecified), peripheral blood plasmacytoid dendritic cell, umbilical cord blood neutrophil granulocyte
https://genevisible.com/tissues/HS/Affymetrix%20Probeset/1552667_a_at
Low-Density Granulocytes Activate T Cells in SLE - The RheumatologistLow-Density Granulocytes Activate T Cells in SLE - The Rheumatologist
Recent research indicates low-density granulocytes from SLE patients exert proinflammatory effects on T cells. Saifur Rahman of the Allan Institute of Immunology, Seattle, and colleagues note the SLE low-density granulocytes dont exhibit myeloid-derived suppressor cell function. The results, published online April 30 in the Annals of Rheumatic Diseases, are consistent with the idea that low-density granulocytes represent a distinct proinflammatory subset with pathogenic potential in SLE patients. This may be, at least in part, because the granulocytes can activate type 1 helper responses.1. In the study, the investigators compared low-density granulocytes from SLE patients with low-density granulocytes from age-matched healthy controls. They confirmed low-density granulocyte prevalence was elevated in SLE relative to healthy controls. They found low-density granulocytes in SLE patients displayed an activated phenotype and were associated with the interferon 21-gene signature and disease ...
https://www.the-rheumatologist.org/article/low-density-granulocytes-activate-t-cells-in-sle/
JCI - Citations to Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesisJCI - Citations to Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis
Granulocytes are pivotal regulators of tissue injury. However, the transcriptional mechanisms that regulate granulopoiesis under inflammatory conditions are poorly understood. Here we show that the transcriptional coregulator B cell leukemia/lymphoma 3 (Bcl3) limits granulopoiesis under emergency (i.e., inflammatory) conditions, but not homeostatic conditions. Treatment of mouse myeloid progenitors with G-CSF - serum concentrations of which rise under inflammatory conditions - rapidly increased Bcl3 transcript accumulation in a STAT3-dependent manner. Bcl3-deficient myeloid progenitors demonstrated an enhanced capacity to proliferate and differentiate into granulocytes following G-CSF stimulation, whereas the accumulation of Bcl3 protein attenuated granulopoiesis in an NF-κB p50-dependent manner. In a clinically relevant model of transplant-mediated lung ischemia reperfusion injury, expression of Bcl3 in recipients inhibited emergency granulopoiesis and limited acute graft damage. These data ...
https://www.jci.org/articles/view/42596/citations
Genevisible - Top 10 tissues for 1552275 s at
Top 10 tissues for 1552275_s_at (Homo sapiens, Affymetrix Probeset): umbilical cord blood neutrophil granulocyte, foreskin-derived neonatal fibroblast, trabecular meshwork cell, tonsillar centrocyte, eye cell, tonsillar centroblast, germinal center B-cell, peripheral blood granulocyte, corpus callosum, subthalamic nucleus
https://genevisible.com/tissues/HS/Affymetrix%20Probeset/1552275_s_at
granulocyte [Operative Neurosurgery]granulocyte [Operative Neurosurgery]
Granulocytes are a category of white blood cells characterized by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN, PML, or PMNL) because of the varying shapes of the nucleus, which is usually lobed into three segments. This distinguishes them from the mononuclear agranulocytes. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes; the other types (…
https://operativeneurosurgery.com/doku.php?id=granulocyte
Granulocytopoiesis definitionGranulocytopoiesis definition
Granulocytopoiesis definition. granulocytopoiesis gran·u·lo·cy·to·poi·e·sis (grānyə-lō-sītə-poi-ēsĭs) n. See granulopoiesis. granu·lo·cyto·poi·etic (-ětĭk) adj.
https://definithing.com/define-dictionary/granulocytopoiesis/
Handbook of Granulocytes: Classification, Toxic Materials Produced and PathologyHandbook of Granulocytes: Classification, Toxic Materials Produced and Pathology
This book also reviews the role of granulocytes on the onset of tissue-destructive diseases when exposed to stress. In addition to the cases of disease, some physiological phenomena are also responsible for the stress-induced granulocytosis. Thus this book will also expands upon the role of granulocytes associated with various diseases and some physiological responses. Other topics discussed in this book include the role of basophil, the minute group of granulocyte, in tropical infections and in immune regulations; polymorphonuclear neutrophilic granulocytes (neutrophils), which are the most dangerous cells in the organism and their role in transient states of autoimmunity, and the effects of nucleoli change during differentiation and maturation of granulocytes ...
http://www.novapublishers.org/catalog/product_info.php?cPath=23_29&products_id=10022
JCI - WelcomeJCI - Welcome
Granulocytes are pivotal regulators of tissue injury. However, the transcriptional mechanisms that regulate granulopoiesis under inflammatory conditions are poorly understood. Here we show that the transcriptional coregulator B cell leukemia/lymphoma 3 (Bcl3) limits granulopoiesis under emergency (i.e., inflammatory) conditions, but not homeostatic conditions. Treatment of mouse myeloid progenitors with G-CSF - serum concentrations of which rise under inflammatory conditions - rapidly increased Bcl3 transcript accumulation in a STAT3-dependent manner. Bcl3-deficient myeloid progenitors demonstrated an enhanced capacity to proliferate and differentiate into granulocytes following G-CSF stimulation, whereas the accumulation of Bcl3 protein attenuated granulopoiesis in an NF-κB p50-dependent manner. In a clinically relevant model of transplant-mediated lung ischemia reperfusion injury, expression of Bcl3 in recipients inhibited emergency granulopoiesis and limited acute graft damage. These data ...
https://www.jci.org/articles/view/42596/cite
Activated granulocyte, SEM - Stock Image C011/1303 - Science Photo LibraryActivated granulocyte, SEM - Stock Image C011/1303 - Science Photo Library
Activated granulocyte. Coloured scanning electron micrograph (SEM) of an activated granulocyte, or polymorphonucleocyte (PMN), white blood cell. Granulocytes are part of the immune system and are characterized by the presence of granules in their cytoplasm. They are released from the bone marrow in response to foreign bodies and infection. Magnification x4000 when printed 10cm wide. - Stock Image C011/1303
http://www.sciencephoto.com/media/432183/view
Granulocyte, SEM - Stock Image C007/4044 - Science Photo LibraryGranulocyte, SEM - Stock Image C007/4044 - Science Photo Library
Granulocyte. Coloured scanning electron micrograph (SEM) of a granulocyte in bone marrow. Granulocytes are a category of white blood cell characterized by the presence of granules in their cytoplasm. Magnification: x8000 when printed at 10 centimetres wide. - Stock Image C007/4044
http://www.sciencephoto.com/media/137027/view
Anti-Granulocyte stimulatin factor (G-CSF) (human) labelled with biotin antibodiesAnti-Granulocyte stimulatin factor (G-CSF) (human) labelled with biotin antibodies
G-CSF; Colonystimulating factor 3 (granulocyte); CSF beta; CSF3;Csfg; Filgrastim; GCSA; GCSF; Lenograstim;Macrophage granulocyte inducer 2; MGI 2;Pluripoietin;Anti-Granulocyte stimulatin factor (G-CSF) (human) labelled with biotin antibodies, P09919
http://www.agrisera.com/en/artiklar/g-csf-granulocyte-colony-stimulating-factor-biotinylated.html
Plus itPlus it
Fucosyltransferase (FT) activity of normal lymphocytes, normal granulocytes, and various types of human leukemic cells and electrofocusing pattern of FT activity in human leukemic cells and normal lymphocytes were examined using asialofetuin as an acceptor. Levels of FT activity in normal lymphocytes were higher than those of normal granulocytes in which FT activity was almost undetectable. The FT activity was higher in blast cells of acute myeloblastic leukemia and chronic myelogenous leukemia in blast crisis than in blast cells of acute lymphoblastic leukemia and the chronic phase of chronic myelogenous leukemia. The level of FT activity was lower in cells of chronic lymphocytic leukemia than that of normal lymphocytes, but it was higher than that of normal granulocytes. Three main isoelectric forms of FT in leukemic blast cells were identified by isoelectrofocusing, and they each had a characteristic focusing point: around pH 4.5 (peak 1); pH 4.9 (peak 2); and pH 5.2 (peak 3). In blast cells ...
http://cancerres.aacrjournals.org/content/47/11/2782
glossary:granulocyte [Lymphedema People]glossary:granulocyte [Lymphedema People]
A leukocyte that has granules in its cytoplasm. A type of white Blood cell that attacks and destroys foreign substances. These are leukocytes which have specific granules. The three different types of granulocytes have different types of specific granules. Granulocytes are spherical in shape, contain nuclei and include neutrophils, eosinophils, and basophils. These cells are part of the formed elements of whole Blood. ...
http://lymphedemapeople.com/wiki/doku.php?id=glossary:granulocyte
Granulocyte products - Products by topic - Products - Miltenyi Biotec - Nederland
Granulocytes are released from the bone marrow and make up the major group of leukocytes in the blood. They represent a heterogeneous population of myeloid innate immune cells characterized by the presence of intracellular granules as well as segmented and lobed nuclei of various shapes. Therefore, they are also referred to as polymorphonuclear cells (PMNC). Recently, along with their well described involvement in inflammation, they have also been shown capable of antigen presentation. Miltenyi Biotec provides numerous tools for granulocyte research, including standardized and automated enrichment of viable and fully functional cells, a pre-requisite to obtain high quality and reproducible data ...
https://www.miltenyibiotec.com/NL-en/products/products-by-topic/granulocytes-products.html
Granulocytes vs. Agranulocytes: What is The Difference? | DiffziGranulocytes vs. Agranulocytes: What is The Difference? | Diffzi
Granulocytes are a type of white blood cells that are present in the cytoplasm in the form of granules. They have many other names in which the most common one is Polymorphonuclear leukocytes; this name became constant because of the different structures of the nucleus that are present in this and connected in three different parts. There are three main types of these. The first one of which is known as neutrophils, that are mostly found in the blood that is flowing in the body and are the most abundant one present among all of them. They make up around 60% of total granulocytes and in one are present in billions just in one liter of blood. The next ones are the eosinophils, they are shaped just like human kidney and have around two to four lobes. They do not have a fixed number in the body because they keep on changing with the blood steam movement. They are essential for the body since they help in killing several kinds of parasites in the body. The last ones are called Basophil; these are the ...
https://diffzi.com/granulocytes-vs-agranulocytes/
Current data on granulocytes donations]. - MyScienceWorkCurrent data on granulocytes donations]. - MyScienceWork
[Current data on granulocytes donations].: Granulocyte transfusion is uncommon but essential for some aplastic patients with major infection and those with sept
https://www.mysciencework.com/publication/show/current-data-granulocytes-donations-94e7d3ea
New neutrophil models for studying host-fungal pathogen interactions | The Journal of ImmunologyNew neutrophil models for studying host-fungal pathogen interactions | The Journal of Immunology
Neutrophils are terminally differentiated phagocytic cells that represent the first line of defense against pathogens. The study of neutrophils in vitro is difficult owing to a short life span. Immortalized cell line models fail to recapitulate all the effector functions of primary cells. Furthermore, the study of murine neutrophil function in vivo is limited by the lack of a model system that can be genetically manipulated (e.g. gene knockout or overexpression) ex vivo. We describe here the development and use of conditionally immortalized granulocyte/macrophage progenitor cells (GMP) capable of unlimited expansion, genetically tractable, and that can be transplanted into mice to study neutrophil function in vivo. These GMP cell lines were derived from UBC-GFP mice by the transduction of an MSCVneo ER-HoxB8 retrovirus such that factor-dependent cell lines emerge in the presence of stem cell factor and b-estradiol (E2). Upon removal of E2 in vitro or transplantation into irradiated recipients, ...
http://www.jimmunol.org/content/196/1_Supplement/205.11
Ligand Announces Positive Preclinical Data on Small-Molecule GCSF Receptor Agonist at the 53rd Annual Meeting of the American...Ligand Announces Positive Preclinical Data on Small-Molecule GCSF Receptor Agonist at the 53rd Annual Meeting of the American...
Further optimization of the LG7455 structure series could lead to a new generation of orally-available small-molecule human GCSFR agonists for the treatment of neutropenia. We are pleased to feature exciting data from one of our novel programs in Ligands internal un-partnered pipeline, said Matthew W. Foehr, Chief Operating Officer of Ligand Pharmaceuticals. Our R&D team continues Ligands rich heritage of being at the forefront of new areas of drug discovery and development. This first-in-class program may provide the most significant innovation in the treatment of neutropenia since the approval of the first GCSF biologic in the early 1990s. About Ligands Small- Molecule GCSF Program GCSF is a glycoprotein growth factor and cytokine produced by different tissues to stimulate the bone marrow to produce granulocytes and stem cells. GCSF acts on its homodimeric receptor (GCSFR) to stimulate proliferation of granulocytic progenitor cells and induce their survival and differentiation into ...
https://www.ligand.com/news-events/press-releases/detail/92/ligand-announces-positive-preclinical-data-on
Effect of beta adrenergic agents on granulocyte deformability - IOS PressEffect of beta adrenergic agents on granulocyte deformability - IOS Press
The effect of beta adrenergic agents on granulocyte deformability was investigated using the filtration technique. Three groups of granulocytes were studied: 1) cells treated with isoproterenol (10−6 to 10−3 M); 2) cells treated with propranolol (10−
https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch7-5-06
In vivo analysis of the role of aberrant histone deacetylase recruitment and RARα blockade in the pathogenesis of acute...In vivo analysis of the role of aberrant histone deacetylase recruitment and RARα blockade in the pathogenesis of acute...
The RARα gene is invariably involved in the APL-associated chromosomal translocations (1, 2). Therefore, alteration of RARα pathway has been thought to play a central role in APL pathogenesis. Indeed, RA inhibits the proliferation of hematopoietic precursors and promotes the terminal granulocytic differentiation of granulocyte/monocyte progenitors and multipotent erythroid/monocytic cells. Vitamin A deficiency, unligated RARα, RARα antagonist, or DN RARα can block myeloid differentiation (23). Moreover, the X-RARα fusion proteins can block differentiation when overexpressed in myeloid leukemia cell lines such as U937 cells and interference with PML function seems not to be required for this function (20, 21). These observations support the notion that DN blockade of the RARα pathway is crucial for APL leukemogenesis. Our in vivo genetic analysis challenges this notion, allowing us to reach three major conclusions.. The first major conclusion is that HDAC1-dependent DN blockade of RARα ...
http://jem.rupress.org/content/203/4/821
Frontiers | Granulocytes: New Members of the Antigen-Presenting Cell Family | ImmunologyFrontiers | Granulocytes: New Members of the Antigen-Presenting Cell Family | Immunology
Granulocytes, the most abundant types of leukocytes, are the first line of defense against pathogen invasion. However, the plasticity and diversity of granulocytes have been increasingly revealed, especially with regards to their versatile functions in orchestrating adaptive immune responses. A substantial body of recent evidence demonstrates that granulocytes can acquire the function as antigen-presenting cells (APC) under pathological or inflammatory conditions. In addition, they can acquire surface expression of MHC class II and co-stimulatory molecules as well as T cell stimulatory behavior when cultured with selected cytokines. The classic view of granulocytes as terminally differentiated, short-lived phagocytes, is therefore changing to phenotypically and functionally heterogeneous cells that are engaged in cross-talk with other leucocyte populations and provide an additional link between innate and adaptive immunity. In this brief review, we summarize the current knowledge on the antigen
https://www.frontiersin.org/articles/10.3389/fimmu.2017.01781/full
DMOZ - Health: Conditions and Diseases: Cancer: Hematologic: Leukemia: Chronic Myelogenous
Chronic myeloid leukaemia (CML) is a rare type of cancer affecting mostly adults. It is a cancer of granulocytes (one of the main types of white blood cells). In CML too many granulocytes are produced and they are released into the blood when they are immature and unable to work properly. The immature white blood cells are known as blasts. The production of other types of blood cells is also disrupted. Normally, white blood cells repair and reproduce themselves in an orderly and controlled manner, but in chronic myeloid leukaemia the process gets out of control and the cells continue to divide and mature abnormally. The disease usually develops very slowly, which is why it is called chronic myeloid leukaemia.
http://dmoztools.net/Health/Conditions_and_Diseases/Cancer/Hematologic/Leukemia/Chronic_Myelogenous/
Inhibition of growth of normal murine granulocytes by cocultured acute by A M. Miller, P L. Page et al.Inhibition of growth of normal murine granulocytes by cocultured acute by A M. Miller, P L. Page et al.
Miller, A M.; Page, P L.; Hartwell, B L.; and Robinson, S H., Inhibition of growth of normal murine granulocytes by cocultured acute leukemic cells. (1977). Subject Strain Bibliography 1977. 31 ...
https://mouseion.jax.org/ssbb1977/31/
MLL-AF9 transforms committed progenitors to leukemia stem cells (Part 2: GSE3722) | Stem Cell CommonsMLL-AF9 transforms committed progenitors to leukemia stem cells (Part 2: GSE3722) | Stem Cell Commons
Leukaemias and other cancers possess a rare population of cells capable of the limitless self-renewal necessary for cancer initiation and maintenance. Here we show that leukaemia stem cells (LSC) can maintain the global identity of the progenitor from which they arose while activating a limited stem-cell- or self-renewal-associated programme. We isolated LSC from leukaemias initiated in committed granulocyte macrophage progenitors through introduction of the MLL-AF9 fusion protein encoded by the t(9;11)(p22;q23). The LSC were capable of transferring leukaemia to secondary recipient mice when only four cells were transferred, and possessed an immunophenotype and global gene expression profile very similar to that of normal granulocyte macrophage progenitors. However, a subset of genes highly expressed in normal haematopoietic stem cells was re-activated in LSC. LSC can thus be generated from committed progenitors without widespread reprogramming of gene expression, and a leukaemia ...
http://stemcellcommons.org/node/7068
Healing Maya
Leukemia affects two types of white blood cells that are responsible for protecting the body from disease: lymphocytes and granulocytes. Leukemias that affect lymphocytes are known as lymphocytic or lymphoblastic, and those that affect granulocytes are called myeloid or myelogenous leukemias ...
http://healingmaya.org
Untitled
A cell type of the immune system. Granulocytes are filled with granules of toxic chemicals that enable them to digest microorganisms. Basophils, neutrophils, and eosinophils are examples of granulocytes. See also Basophil; Eosinophil; Neutrophil ...
http://treasuresoftheinternet.org/dictionary/g/gr/granulocyte.shtml
Pays-Bas | FR<i class= sysmexAtomIcon sysi-globe-wireframe sysmexAtomIcon icon ...Pays-Bas | FR<i class= sysmexAtomIcon sysi-globe-wireframe sysmexAtomIcon icon ...
What is an IG count? With the exception of blood from neonates or pregnant women, the appearance of immature granulocytes in the peripheral blood indicates an early-stage response to infection, inflammation or other stimuli of the bone marrow. Being able to detect them quickly and reliably opens doors to new diagnostic possibilities for related disorders. Current areas of research regarding the diagnostic significance of circulating immature granulocytes focus on the early and rapid discrimination of bacterial from viral infections, particularly in children, recognising bacterial infection in neonates, and the early recognition of bacterial infection and sepsis in adults, which is of vital importance in particular for intensive care patients. Valuable information for immediate action - at no additional cost IG counts are relevant especially for patients who are highly susceptible to infections because of a suppressed immune system and because the increased IG count indicates the severity of the ...
https://www.sysmex.nl/nl-fr/academie/knowledge-centre/parameter/immature-granulocyte-ig-count.html
GastroHep News StoryGastroHep News Story
The team used intervention, granulocyte/monocyte apheresis with the Adacolumn Apheresis System or sham apheresis in a 2:1 ratio for 9 weeks of treatment in a North American pivotal study.. The research team also conducted a smaller, companion study of identical design in Europe and Japan including 47 patients.. The researchers found in the pivotal study, that clinical remission rates were 17% and 11% for the granulocyte/monocyte apheresis- and sham-treatment groups, respectively.. The team observed clinical response in 44% and 39% of the granulocyte/monocyte apheresis- and sham-treatment groups, respectively.. Similar changes were observed for the apheresis- and sham-treatment groups for endoscopic remission and response, and changes in Mayo and quality-of-life scores.. The researchers noted that the companion study and pooled data from both studies also yielded similar results.. Dr Sands team concluded, In this study, granulocyte/monocyte apheresis was well tolerated but did not demonstrate ...
https://www.gastrohep.com/news/news.asp?id=105938
KAKEN - Research Projects | Gene Expression of Myeloperoxidase during Granulocyte Differentiation (KAKENHI-PROJECT-05680549)KAKEN - Research Projects | Gene Expression of Myeloperoxidase during Granulocyte Differentiation (KAKENHI-PROJECT-05680549)
Principal Investigator：YAMADA Michiyuki, Project Period (FY)：1993 - 1994, Research Category：Grant-in-Aid for General Scientific Research (C), Research Field：Functional biochemistry
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05680549/
Overexpression of DR-nm23, a protein encoded by a member of the nm23 gene family, inhibits granulocyte differentiation and...Overexpression of DR-nm23, a protein encoded by a member of the nm23 gene family, inhibits granulocyte differentiation and...
Sigma-Aldrich offers abstracts and full-text articles by [D Venturelli, R Martinez, P Melotti, I Casella, C Peschle, C Cucco, G Spampinato, Z Darzynkiewicz, B Calabretta].
https://www.sigmaaldrich.com/catalog/papers/7638209
Recovery, Structure and Function of Dog Granulocytes After Freeze Preservation with Dimethylsulfoxide.
The recovery, structure and function of dog granulocytes were determined before and after freeze preservation. Leucocytes were isolated from defibrinated or anticoagulated whole blood and subsequent erythrocyte sedimentation on a column of 2:1 dextran (6%)-Isopaque (33.9%). Granulocytes isolated by these procedures were examined for changes in O2 consumption associated with phagocytosis, in vitro directed migration (chemotaxis), bactericidal activity, and ultrastructure before and after freezing. Granulocytes were frozen in DMSO (7.5%) and autologous serum of HBSS and 20% autologous serum at the rate of -1 C/min to -80 C and stored in liquid N2 vapor. It is concluded that dog granulocytes freeze preserved by these methods are functional in vitro, but that phagocytic, directed migration, and bactericidal functions and ultrastructure are impaired to different degrees, according to the method of isolation and preparation for storage. These results indicate the need for continued investigation on the
http://www.dtic.mil/docs/citations/ADA033508
Difference Between CML and CLL | CML vs CLLDifference Between CML and CLL | CML vs CLL
What is the difference between CML and CLL? Granulocytes are the cancerous cells in CML whereas lymphocytes are the cancerous cells in CLL. CML is a member of..
http://www.differencebetween.com/difference-between-cml-and-vs-cll/
Band-form granulocyte | Article about band-form granulocyte by The Free DictionaryBand-form granulocyte | Article about band-form granulocyte by The Free Dictionary
Looking for band-form granulocyte? Find out information about band-form granulocyte. A leukocyte containing granules in the cytoplasm. Also known as granular leukocyte; polymorph; polymorphonuclear leukocyte Explanation of band-form granulocyte
https://encyclopedia2.thefreedictionary.com/band-form+granulocyte
Rapid and persistent monocyte and granulocyte infiltrat | Open-iRapid and persistent monocyte and granulocyte infiltrat | Open-i
Rapid and persistent monocyte and granulocyte infiltration is detected at the treatment site following EP-enhanced plasmid delivery. Hematoxylin and eosin (H&am
https://openi.nlm.nih.gov/detailedresult.php?img=PMC4494224_vaccines-01-00384-g004&req=4
Anti-Siglec-F MicroBeads, mouse | Granulocytes and myeloid cells | MicroBeads and Isolation Kits | Cell separation reagents |...
The Anti-Siglec-F MicroBeads, mouse have been developed for the positive selection or depletion of cells expressing mouse Siglec-F antigen from mouse peripheral blood (eosinophils, minor fraction of neutrophils) or mouse lung single-cell suspensions (eosinophils and alveolar macrophages). | Canada
https://www.miltenyibiotec.com/CA-en/products/anti-siglec-f-microbeads-mouse.html
Monocyte/Granulocyte Antibodies: Novus BiologicalsMonocyte/Granulocyte Antibodies: Novus Biologicals
Monocyte/Granulocyte Antibodies available through Novus Biologicals. Browse our Monocyte/Granulocyte Antibody catalog backed by our Guarantee+.
https://www.novusbio.com/primary-antibodies/monocyte-granulocyte
How do granulocytes affect my ATAC data? - 10X GenomicsHow do granulocytes affect my ATAC data? - 10X Genomics
Question: How do granulocytes affect my ATAC data? Answer: Granulocytes, specifically neutrophils, can be present in PBMC and BMMC...
https://kb.10xgenomics.com/hc/en-us/articles/360046631331
Surekha M. Zingde, Ph.D. profile in India Cancer Research Database
Profiling of oral tumors by proteomics and immunoproteomics towards discovery of biomarkers and therapeutic targets, Biochemistry & Molecular Biology of granulocyte membrane proteins, Signal transduction in Granulocytes, Protein Kinase C in erythrocytes,
http://incredb.org/investigator.php?incredb_id=9
Reticular dysgenesis-associated AK2 protects hematopoietic stem and progenitor cell development from oxidative stress | JEMReticular dysgenesis-associated AK2 protects hematopoietic stem and progenitor cell development from oxidative stress | JEM
RD is a rare and life-threatening disease affecting infants in the immediate newborn period. Although previous studies have shown that mutations in the AK2 gene are responsible for RD, the rarity of this condition and the lack of suitable animal and cellular models have represented significant obstacles to defining its molecular and cellular pathophysiology. Here we describe ak2 mutant zebrafish and patient-derived iPSCs as platforms to investigate the effects of AK2 mutations on hematopoiesis and to explore novel therapeutic approaches.. Manipulations of AK activity in different organisms results in impaired adenine nucleotide homeostasis, block of ATP export from mitochondria, and reduced cell growth and metabolism, as well as increased susceptibility to metabolic stress (Bandlow et al., 1988; Janssen et al., 2000; Pucar et al., 2000, 2002). Impairment of AK2 activity in humans leads to the severe lymphocyte and granulocyte differentiation defects and sensorineural deafness characteristic for ...
http://jem.rupress.org/content/early/2015/06/30/jem.20141286
DIFF ChannelDIFF Channel
A surfactant in Stromatolyser-4DL causes lysis of red blood cells. At the same time, the cell membranes of white blood cells are slightly perforated.. The polymethine fluorescence marker Stromatolyser-4DS migrates into the WBC and binds in particular to nucleic acids and cell organelles. Additionally an organic acid in the Stromatolyser-4DL binds specifically to the granules in eosinophils as a result of which they can be differentiated from neutrophils by means of a higher side scatter signal.. Our XE- and XT-Series analysers provide five populations of white blood cells in the DIFF scattergram, including the Immature Granulocyte population.. The XS analysers scattergrams displays the following five populations: lymphocytes, monocytes, basophils, eosinophils and neutrophils. ...
https://www.sysmex-europe.com/n/academy/clinic-laboratory/analyser-channels/diff-channel.html
Plus itPlus it
We investigated interactions between breast cancer cells and CD45-CD34+ WAT progenitors because the latter promote tumor growth, metastasis, and angiogenesis in preclinical breast cancer models (15, 16). We found that the proteins GM-CSF and MMP9 were significantly upregulated in murine and human WAT progenitors on exposure to a variety of breast cancer cell types in vitro and in vivo. These proteins were not upregulated in other WAT cells (e.g., hematopoietic cells). Both ASCs and ECs-constituents of the CD45-CD34+ WAT fraction-produced GM-CSF and MMP9 when cocultured with breast cancer cells, consistent with previous findings that ASCs and ECs cooperate to support breast cancer growth, angiogenesis, and metastatic spread (16).. GM-CSF is a growth factor for hematopoietic and immune cells, which mobilizes stem cells and induces macrophage/granulocyte differentiation (33). Other roles include the regulation of inflammation and autoimmunity (34). MMP9 is a type IV collagenase whose increased ...
http://cancerres.aacrjournals.org/content/77/18/5169
NecrosisNecrosis
Necrosis no symptoms malaise Monocyte % Monocyte Absolute Immature Granulocyte % Neutrophil % Neutrophil Absolute WBC ESR - Sed Rate LDH Lactate dehydrogenase
http://smrtx.com/00144_Necrosis.htm
GranulocyteGranulocyte
... granulocyte levels less than 5% of normal) and neutropenia (deficiency of neutrophil granulocytes). Granulocytes live only one ... polymorphonuclear granulocyte refers specifically to "neutrophil granulocytes", the most abundant of the granulocytes; the ... Granulocytes are produced via granulopoiesis in the bone marrow. There are four types of granulocytes (full name ... the most abundant granulocyte is the neutrophil granulocyte, which has neutrally staining cytoplasmic granules.[citation needed ...
https://en.wikipedia.org/wiki/Granulocyte
Granulocyte transfusionGranulocyte transfusion
A granulocyte transfusion is a medical procedure in which granulocytes (a type of white blood cell) are infused into a person's ... Donors may be given corticosteroids or granulocyte colony-stimulating factor to increase their granulocyte count prior to the ... led to a renewal of interest in granulocyte transfusions. If an ABO-compatible donor cannot be found, the granulocytes can ... Granulocyte transfusion is still used in some cases, such as when a person has severe neutropenia and an infection that is life ...
https://en.wikipedia.org/wiki/Granulocyte_transfusion
Granulocyte colony-stimulating factorGranulocyte colony-stimulating factor
Sallerfors B, Olofsson T (October 1992). "Granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony- ... Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein ... Mouse granulocyte-colony stimulating factor (G-CSF) was first recognised and purified in Walter and Eliza Hall Institute, ... Granulocyte+Colony-Stimulating+Factor at the US National Library of Medicine Medical Subject Headings (MeSH) Overview of all ...
https://en.wikipedia.org/wiki/Granulocyte_colony-stimulating_factor
Granulocyte colony-stimulating factor receptorGranulocyte colony-stimulating factor receptor
The granulocyte colony-stimulating factor receptor (G-CSF-R) also known as CD114 (Cluster of Differentiation 114) is a protein ... The granulocyte colony-stimulating factor receptor is present on precursor cells in the bone marrow, and, in response to ... G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). The G-CSF receptors belongs to a ... Granulocyte colony-stimulating factor receptor has been shown to interact with Grb2, HCK and SHC1. Cluster of differentiation ...
https://en.wikipedia.org/wiki/Granulocyte_colony-stimulating_factor_receptor
Granulocyte-macrophage colony-stimulating factorGranulocyte-macrophage colony-stimulating factor
CFU-GM Filgrastim (Neupogen, a granulocyte colony-stimulating factor (G-CSF) analog) Granulocyte-macrophage colony-stimulating ... Root RK, Dale DC (March 1999). "Granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor: ... Human granulocyte-macrophage colony-stimulating factor is glycosylated in its mature form. GM-CSF was first cloned in 1985, and ... Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric ...
https://en.wikipedia.org/wiki/Granulocyte-macrophage_colony-stimulating_factor
Granulocyte-macrophage colony-stimulating factor receptorGranulocyte-macrophage colony-stimulating factor receptor
The granulocyte-macrophage colony-stimulating factor receptor also known as CD116 (Cluster of Differentiation 116), is a ... Williams WV, VonFeldt JM, Rosenbaum H, Ugen KE, Weiner DB (Oct 1994). "Molecular cloning of a soluble form of the granulocyte- ... Crosier KE, Wong GG, Mathey-Prevot B, Nathan DG, Sieff CA (Sep 1991). "A functional isoform of the human granulocyte/macrophage ... Gearing DP, King JA, Gough NM, Nicola NA (Dec 1989). "Expression cloning of a receptor for human granulocyte-macrophage colony- ...
https://en.wikipedia.org/wiki/Granulocyte-macrophage_colony-stimulating_factor_receptor
Thomas P. StosselThomas P. Stossel
Functions of granulocytes. In: Williams WJ, Beutler E, Erslev AJ, Rundles RW, eds. Hematology. New York: McGraw-Hill, 1977:685- ... The Granulocyte: Function and Clinical Utilization. New York: Alan R. Liss, 1977; 13: 87-102. 20. Stossel TP, Hartwig JH, Boxer ... Qualitative abnormalities of granulocytes. In: Williams WJ, Beutler E, Erslev AJ, Rundles RW, eds. Hematology. New York: McGraw ... Granulocyte Physiology Function and Dysfunction. Washington D.C.: American Association of Blood Banks, 1979:49-61. 38. Stossel ...
https://en.wikipedia.org/wiki/Thomas_P._Stossel
Estradiol propoxyphenylpropionateEstradiol propoxyphenylpropionate
ISBN 978-1-4757-2085-3. Baker AP, Bergman F, Paul KG (April 1967). "Studies of eosinophil granulocytes. 3. Steroid hormones and ...
https://en.wikipedia.org/wiki/Estradiol_propoxyphenylpropionate
ThymolphthaleinThymolphthalein
Localization of Pyrogen in Granulocytes". J. Exp. Med. 131 (4): 701-9. doi:10.1084/jem.131.4.701. PMC 2138774. PMID 5430784. ...
https://en.wikipedia.org/wiki/Thymolphthalein
Glitter cellGlitter cell
Cell biology, Granulocytes, Phagocytes, Human cells). ...
https://en.wikipedia.org/wiki/Glitter_cell
AgranulocytosisAgranulocytosis
Transfusion of granulocytes would be a solution to the problem. However, granulocytes live only ~10 hours in the circulation ( ... Neutropenia indicates a deficiency of neutrophils (the most common granulocyte cell) only. To be precise, neutropenia is the ... The following terms can be used to specify the type of granulocyte referenced: Inadequate numbers of neutrophils: neutropenia ( ... There can also be isolated neutropenias where only differentiated granulocyte precursors are affected as in the case of ...
https://en.wikipedia.org/wiki/Agranulocytosis
CCL17CCL17
It does not interact with granulocytes. It acts as a powerful chemoattractant to T-helper cells and T-regulatory cells because ... Granulocyte-macrophage colony-stimulating factor (GM-CSF) upregulates CCL17 production in monocytes and macrophages. Dendritic ...
https://en.wikipedia.org/wiki/CCL17
Hemocyte (invertebrate immune system cell)Hemocyte (invertebrate immune system cell)
Granulocytes are the most abundant cell type. They rapidly attach to foreign surfaces and readily engage in phagocytosis. ... Prohemocytes are small cells of unknown function, which may result from the asymmetric mitosis of granulocytes. Schlenke TA, ... In mosquitoes, hemocytes are functionally divided into three populations: granulocytes, oenocytoids and prohemocytes. ...
https://en.wikipedia.org/wiki/Hemocyte_(invertebrate_immune_system_cell)
Zanvil A. CohnZanvil A. Cohn
Extracellular cytolysis by activated macrophages and granulocytes. II . Hydrogen peroxide as a mediator of cytotoxicity. J. Exp ...
https://en.wikipedia.org/wiki/Zanvil_A._Cohn
Cathepsin GCathepsin G
Klickstein LB, Kaempfer CE, Wintroub BU (December 1982). "The granulocyte-angiotensin system. Angiotensin I-converting activity ...
https://en.wikipedia.org/wiki/Cathepsin_G
EosinophiliaEosinophilia
Eosinophils, like other granulocytes, contain granules (or sacs) filled with digestive enzymes and cytotoxic proteins which ... Eosinophils are one form of terminally differentiated granulocytes; they function to neutralize invading microbes, primarily ... granulocyte macrophage colony stimulating factor, interleukin 3, interleukin 5) that: a) cause bone marrow precursor cells, i.e ...
https://en.wikipedia.org/wiki/Eosinophilia
Megaloblastic anemiaMegaloblastic anemia
Neutrophil granulocytes may show multisegmented nuclei ("senile neutrophil"). This is thought to be due to decreased production ...
https://en.wikipedia.org/wiki/Megaloblastic_anemia
Chronic myelomonocytic leukemiaChronic myelomonocytic leukemia
Other features may include; leukocytosis (50% of cases); left shift and dysplasia of monocytes and granulocytes; presence of ...
https://en.wikipedia.org/wiki/Chronic_myelomonocytic_leukemia
Baccharis genistelloidesBaccharis genistelloides
ISBN 978-3-7347-3299-7. Granulocytes: Advances in Research and Application: 2011 Edition. ScholarlyEditions. 2012-01-09. p. 83 ...
https://en.wikipedia.org/wiki/Baccharis_genistelloides
Metorchis conjunctusMetorchis conjunctus
High concentrations of eosinophil granulocytes are in blood. Also, higher concentrations of liver enzymes are seen. When ...
https://en.wikipedia.org/wiki/Metorchis_conjunctus
Blood cellBlood cell
White blood cells are divided into granulocytes and agranulocytes, distinguished by the presence or absence of granules in the ... Granulocytes include basophils, eosinophils, neutrophils, and mast cells. Agranulocytes include lymphocytes and monocytes. The ...
https://en.wikipedia.org/wiki/Blood_cell
LeishmaniaLeishmania
2003). "Neutrophil granulocytes - Trojan horses for Leishmania major and other intracellular microbes?". Trends in Microbiology ... Activated PMN secrete chemokines, IL-8 particularly, to attract further granulocytes and stimulate phagocytosis. Further, L. ... The lifespan of neutrophil granulocytes is quite short. They circulate in bloodstream for about 6 to 10 hours after leaving ... They use the willingly phagocytosing polymorphonuclear neutrophil granulocytes (PMNs) rigorously as a tricky hideout, where ...
https://en.wikipedia.org/wiki/Leishmania
Eosinophil-derived neurotoxinEosinophil-derived neurotoxin
The protein encoded by this gene is found in eosinophil granulocytes. It is closely related to the eosinophil cationic protein ... 1993). "Eosinophil granule proteins in peripheral blood granulocytes". J. Leukoc. Biol. 52 (6): 611-8. doi:10.1002/jlb.52.6.611 ...
https://en.wikipedia.org/wiki/Eosinophil-derived_neurotoxin
CD109CD109
". "IPD - HPA Sequence Database, EBI". Lucas GF, Metcalfe P (2000). "Platelet and granulocyte glycoprotein polymorphisms". ...
https://en.wikipedia.org/wiki/CD109
Ehrlichia ewingiiEhrlichia ewingii
Ehrlichia canis is usually found in lymphocytes and monocytes, but this new strain was found in granulocytes. Due to the ... on June 18, 1987 using a canine diagnosed with granulocytic ehrlichiosis: an infection of the granulocytes by a member of the ... This bacterium then infects granulocytes in canines and humans. Currently, Ehrlichia ewingii cannot be cultivated in a number ...
https://en.wikipedia.org/wiki/Ehrlichia_ewingii
Mir-190 microRNA precursor familyMir-190 microRNA precursor family
"MicroRNA expression profile in granulocytes from primary myelofibrosis patients". Experimental Hematology. 35 (11): 1708-18. ...
https://en.wikipedia.org/wiki/Mir-190_microRNA_precursor_family
Myelophthisic anemiaMyelophthisic anemia
These immature granulocytes are seen in peripheral blood smears. Diagnosis is confirmed when a bone marrow biopsy demonstrates ... or immature granulocyte precursor cells which indicates leukoerythroblastosis is occurring because the displaced hematopoietic ...
https://en.wikipedia.org/wiki/Myelophthisic_anemia
TRPC6TRPC6
Heiner I, Eisfeld J, Lückhoff A (2004). "Role and regulation of TRP channels in neutrophil granulocytes". Cell Calcium. 33 (5-6 ...
https://en.wikipedia.org/wiki/TRPC6
TRPV6TRPV6
Heiner I, Eisfeld J, Lückhoff A (2004). "Role and regulation of TRP channels in neutrophil granulocytes". Cell Calcium. 33 (5-6 ...
https://en.wikipedia.org/wiki/TRPV6
Toxic vacuolationToxic vacuolation
The finding is also associated with bacterial infection, alcohol toxicity, liver failure, and treatment with granulocyte colony ... Hematology and Clinical Microscopy Committee (2019). "Blood cell identification: Granulocytes and Monocytes: Neutrophil, toxic ... with neutral lipid storage disease may exhibit persistent lipid-filled vacuoles in neutrophils and other granulocytes, which is ...
https://en.wikipedia.org/wiki/Toxic_vacuolation
Granulocyte: MedlinePlus Medical EncyclopediaGranulocyte: MedlinePlus Medical Encyclopedia
The specific types of granulocytes are neutrophils, eosinophils, and basophils. ... Granulocytes are a type of white blood cell that has small granules. These granules contain proteins. ... Granulocytes, specifically neutrophils, help the body fight bacterial infections. The number of granulocytes in the body ... Granulocytes are a type of white blood cell that has small granules. These granules contain proteins. The specific types of ...
https://medlineplus.gov/ency/article/003440.htm
Browsing by Subject Granulocyte Colony Stimulating Factor, RecombinantBrowsing by Subject "Granulocyte Colony Stimulating Factor, Recombinant"
Report on a collaborative study for proposed 2nd international standard for granulocyte colony stimulating factor (‎G-CSF)‎  ... Browsing by Subject "Granulocyte Colony Stimulating Factor, Recombinant". 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. ...
https://apps.who.int/iris/browse?authority=Granulocyte+Colony+Stimulating+Factor%2C+Recombinant&type=mesh
Ver por tema Granulocyte Colony Stimulating Factor, RecombinantVer por tema "Granulocyte Colony Stimulating Factor, Recombinant"
Report on a collaborative study for proposed 2nd international standard for granulocyte colony stimulating factor (‎G-CSF)‎  ... Ver por tema "Granulocyte Colony Stimulating Factor, Recombinant". 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R. S ...
https://extranet.who.int/iris/restricted/browse?locale-attribute=pt&type=mesh&authority=Granulocyte+Colony+Stimulating+Factor,+Recombinant
Granulocyte colony stimulating factor treatment following a heart attack | CochraneGranulocyte colony stimulating factor treatment following a heart attack | Cochrane
Granulocyte colony stimulating factor treatment following a heart attack. People who suffer a heart attack (due to a blockage ... These cells can be mobilized from the bone marrow by factors such as granulocyte colony stimulating factor (G-CSF). ... These cells could be mobilized to the heart with agents such as granulocyte colony stimulating factor (G-CSF). ... To assess the effects of stem cell mobilization following granulocyte colony stimulating factor therapy in patients with acute ...
https://www.cochrane.org/CD008844/VASC_granulocyte-colony-stimulating-factor-treatment-following-heart-attack
StemSpan™ Human Myeloid (Granulocyte) Cytokine Supplement | STEMCELL TechnologiesStemSpan™ Human Myeloid (Granulocyte) Cytokine Supplement | STEMCELL Technologies
Recombinant human granulocyte colony-stimulating factor (G-CSF). • Recombinant human granulocyte/macrophage colony-stimulating ... Serum-free culture supplement for expansion and differentiation of human granulocytes. StemSpan™ Myeloid Expansion Supplement ( ... Promotes the production of thousands of CD15+ granulocytes per input CD34+ human CB cell in 14-day liquid cultures. ... StemSpan™ Media and Supplements for the Expansion and Differentiation of Granulocytes and Monocyte... ...
https://www.stemcell.com/products/stemspan-myeloid-expansion-supplement-100x.html
Hairy Cell Leukemia Medication: Purine Analogues, Interferons, Recombinant Granulocyte Colony-Stimulating Factors (G-CSFs),...Hairy Cell Leukemia Medication: Purine Analogues, Interferons, Recombinant Granulocyte Colony-Stimulating Factors (G-CSFs),...
Recombinant Granulocyte Colony-Stimulating Factors (G-CSFs). Class Summary. Because treatment of hairy cell leukemia is ...
https://emedicine.medscape.com/article/200580-medication
Granulocyte Colony-Stimulating Factor | Harvard Catalyst Profiles | Harvard CatalystGranulocyte Colony-Stimulating Factor | Harvard Catalyst Profiles | Harvard Catalyst
Effect of granulocyte colony-stimulating factor on toxicities after CAR T cell therapy for lymphoma and myeloma. Blood Cancer J ... "Granulocyte Colony-Stimulating Factor" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, ... Granulocyte colony-stimulating factor attenuates liver damage by M2 macrophage polarization and hepatocyte proliferation in ... Outcomes of granulocyte colony-stimulating factor use in pediatric kidney transplant recipients: A Pediatric Nephrology ...
https://connects.catalyst.harvard.edu/Profiles/display/Concept/Granulocyte%20Colony-Stimulating%20Factor
Collection of Granulocytes by Apheresis of Healthy Donors Stimulated With Filgrastim (G-CSF) and Dexamethasone | Clinical...Collection of Granulocytes by Apheresis of Healthy Donors Stimulated With Filgrastim (G-CSF) and Dexamethasone | Clinical...
Collection of Granulocytes by Apheresis of Healthy Donors Stimulated With Filgrastim (G-CSF) and Dexamethasone ... Collection of Granulocytes by Apheresis of Healthy Donors Stimulated With Filgrastim (G-CSF) and Dexamethasone * ... Participants will donate granulocytes by apheresis a maximum of 12 times in 1 year. Donations will not usually be requested ... Granulocyte components are not recognized as a licensed blood component by the Food and Drug Administration (FDA), and neither ...
https://www.centerwatch.com/clinical-trials/listings/162791/collection-of-granulocytes-by-apheresis-of-healthy-donors-stimulated-with-filgrastim-g-csf-and-dexamethasone/
What does it mean if your immature granulocytes are high? - Bankruptingamerica.orgWhat does it mean if your immature granulocytes are high? - Bankruptingamerica.org
Immature granulocytes (IG) level in peripheral blood is used as early sign of infection. On the other hand, IG could be ... Background: Immature granulocytes (IG) level in peripheral blood is used as early sign of infection. On the other hand, IG ... What does high Gran mean in a blood test? The White Blood Count (WBC) in a blood test result is broken down into Granulocytes ( ... What is grans absolute in blood test? Granulocytes, absolute (GR, abs) or. percentage (GR, pct) Measures the number or ...
https://bankruptingamerica.org/what-does-it-mean-if-your-immature-granulocytes-are-high
View source for Granulocyte macrophage colony stimulating factor - wikidocView source for Granulocyte macrophage colony stimulating factor - wikidoc
View source for Granulocyte macrophage colony stimulating factor. ← Granulocyte macrophage colony stimulating factor ... distinguish,granulocyte colony-stimulating factor}} {{drugbox , IUPAC_name = Human granulocyte macrophage colony stimulating ... Return to Granulocyte macrophage colony stimulating factor.. Retrieved from "https://www.wikidoc.org/index.php/Granulocyte_ ... granulocyte]]s ([[neutrophil]]s, [[eosinophil]]s, and [[basophil]]s) and [[monocyte]]s. Monocytes exit the circulation and ...
https://wikidoc.org/index.php?title=Granulocyte_macrophage_colony_stimulating_factor&action=edit
Whole Lung Lavage (WLL)/Inhaled Granulocyte-macrophage Colony-stimulating Factor (GM-CSF) in Autoimmune Pulmonary Alveolar...Whole Lung Lavage (WLL)/Inhaled Granulocyte-macrophage Colony-stimulating Factor (GM-CSF) in Autoimmune Pulmonary Alveolar...
Aerosol granulocyte-macrophage colony-stimulating factor for pulmonary alveolar proteinosis. Eur Respir J. 2006 Mar;27(3):585- ... Whole Lung Lavage (WLL)/Inhaled Granulocyte-macrophage Colony-stimulating Factor (GM-CSF) in Autoimmune Pulmonary Alveolar ...
https://clinicaltrials.gov/ct2/show/NCT00901511
CHANGES IN THE PROGRAMMED CELL DEATH OF POLYMORPHONUCLEAR GRANULOCYTES WITH AGING UNDER VARIOUS STIMULATIONS | Biochemical...CHANGES IN THE PROGRAMMED CELL DEATH OF POLYMORPHONUCLEAR GRANULOCYTES WITH AGING UNDER VARIOUS STIMULATIONS | Biochemical...
CHANGES IN THE PROGRAMMED CELL DEATH OF POLYMORPHONUCLEAR GRANULOCYTES WITH AGING UNDER VARIOUS STIMULATIONS T. Fülöp, Jr; T. ... T. Fülöp, C. Leblanc, C. Fouquet, G. Lacombe; CHANGES IN THE PROGRAMMED CELL DEATH OF POLYMORPHONUCLEAR GRANULOCYTES WITH AGING ...
https://portlandpress.com/biochemsoctrans/article-abstract/24/4/620S/93834/CHANGES-IN-THE-PROGRAMMED-CELL-DEATH-OF?redirectedFrom=PDF
Use of recombinant human granulocyte-macrophage colony stimulating factor in an infant with reticular dysgenesisUse of recombinant human granulocyte-macrophage colony stimulating factor in an infant with reticular dysgenesis
... Mar 1, 1994 ... We present the case of a 2-month-old infant with reticular dysgenesis who was treated with recombinant granulocyte-macrophage ...
https://www.cun.es/en/research/scientific-publications/use-of-recombinant-human-granulocyte-macrophage-colony-stimulating-factor-in-an-infant-with-reticular-dysgenesis
Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy. | Scholars@DukeGranulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy. | [email protected]
Granulocyte deformability and F-actin content: relationship to diabetes mellitus and retinopathy. ... Diabetic granulocytes were only 9% +/- 22% less deformable than normal granulocytes (p = 0.16). Deformability was increased in ... To determine whether subjects with diabetes mellitus have less deformable granulocytes than healthy subjects, granulocyte ... Although granulocytes may be important in the pathogenesis of diabetic retinopathy, granulocyte deformability (measured by mean ...
https://scholars.duke.edu/display/pub681048
Sonnet Biotherapeutics Announces Positive Preclinical Results from an Initial Efficacy Study Exploring the Combination of...Sonnet Biotherapeutics Announces Positive Preclinical Results from an Initial Efficacy Study Exploring the Combination of...
Biotherapeutics Announces Positive Preclinical Results from an Initial Efficacy Study Exploring the Combination of Granulocyte- ...
https://www.accesswire.com/598073/online-newsroom
WHO EMRO | Study of aerobic granulocyte functional activity in the presence of a radiosensitizer (metronidazole) | Volume 15,...
Study of aerobic granulocyte functional activity in the presence of a radiosensitizer (metronidazole) ... Study of aerobic granulocyte functional activity in the presence of a radiosensitizer (metronidazole) ... A study of the redox and kinetic functional activity of respiratory bursts in normal granulocytes of human whole blood by means ... Eastern Mediterranean Health Journal , All issues , Volume 15, 2009 , Volume 15, issue 1 , Study of aerobic granulocyte ...
http://www.emro.who.int/emhj-volume-15-2009/volume-15-issue-1/article26.html
The chemoattractants, IL-8 and formyl-methionyl-leucyl-phenylalanine, regulate granulocyte colony-stimulating factor signaling...
Dive into the research topics of The chemoattractants, IL-8 and formyl-methionyl-leucyl-phenylalanine, regulate granulocyte ... The chemoattractants, IL-8 and formyl-methionyl-leucyl-phenylalanine, regulate granulocyte colony-stimulating factor signaling ... The chemoattractants, IL-8 and formyl-methionyl-leucyl-phenylalanine, regulate granulocyte colony-stimulating factor signaling ... title = "The chemoattractants, IL-8 and formyl-methionyl-leucyl-phenylalanine, regulate granulocyte colony-stimulating factor ...
https://pure.qub.ac.uk/en/publications/the-chemoattractants-il-8-and-formyl-methionyl-leucyl-phenylalani
A randomised trial of granulocyte-macrophage colony-stimulating factor for neonatal sepsis: outcomes at 2 years | ADC Fetal &...A randomised trial of granulocyte-macrophage colony-stimulating factor for neonatal sepsis: outcomes at 2 years | ADC Fetal &...
A randomised trial of granulocyte-macrophage colony-stimulating factor for neonatal sepsis: outcomes at 2 years ... A randomised trial of granulocyte-macrophage colony-stimulating factor for neonatal sepsis: outcomes at 2 years ... A randomised trial of granulocyte-macrophage colony-stimulating factor for neonatal sepsis: outcomes at 2 years ...
https://fn.bmj.com/content/98/1/F46.share
Granulocyte-Macrophage Colony-Stimulating Factor in the Management of Severe Ecthyma Gangrenosum Related to Myelodysplastic...Granulocyte-Macrophage Colony-Stimulating Factor in the Management of Severe Ecthyma Gangrenosum Related to Myelodysplastic...
Granulocyte-macrophage colony stimulating factor for myelodysplastic syndrome . Blood . 1990;76( (suppl 1) ):318. 12. ... 1992;27:415-418.Crossref 4. Metcalf D. Control of granulocytes and macrophages . Science . 1991;254:529-533.Crossref 5. Ruef C ... 1989;110:976-984.Crossref 8. Monroy R, Davis T, MacVittie T. Granulocyte-macrophage colony stimulating factor . Clin Immunol ... Treatment of myelodysplastic syndromes with recombinant human granulocyte colony stimulating factor . Ann Intern Med. ...
https://www.deepdyve.com/lp/american-medical-association/granulocyte-macrophage-colony-stimulating-factor-in-the-management-of-M3vwCurj7z
Esophageal Expression of Active IκB Kinase-β in Mice Up-Regulates Tumor Necrosis Factor and Granulocyte-Macrophage Colony...
T1 - Esophageal Expression of Active IκB Kinase-β in Mice Up-Regulates Tumor Necrosis Factor and Granulocyte-Macrophage Colony- ... Esophageal Expression of Active IκB Kinase-β in Mice Up-Regulates Tumor Necrosis Factor and Granulocyte-Macrophage Colony- ... Esophageal Expression of Active IκB Kinase-β in Mice Up-Regulates Tumor Necrosis Factor and Granulocyte-Macrophage Colony- ... title = "Esophageal Expression of Active IκB Kinase-β in Mice Up-Regulates Tumor Necrosis Factor and Granulocyte-Macrophage ...
https://www.scholars.northwestern.edu/en/publications/esophageal-expression-of-active-i%CE%BAb-kinase-%CE%B2-in-mice-up-regulates
Tumor-infiltrating lymphocytes derived from select B-cell lymphomas secrete granulocyte-macrophage colony-stimulating factor...
Dive into the research topics of Tumor-infiltrating lymphocytes derived from select B-cell lymphomas secrete granulocyte- ... Tumor-infiltrating lymphocytes derived from select B-cell lymphomas secrete granulocyte-macrophage colony-stimulating factor ... Tumor-infiltrating lymphocytes derived from select B-cell lymphomas secrete granulocyte-macrophage colony-stimulating factor ... Tumor-infiltrating lymphocytes derived from select B-cell lymphomas secrete granulocyte-macrophage colony-stimulating factor ...
https://jhu.pure.elsevier.com/en/publications/tumor-infiltrating-lymphocytes-derived-from-select-b-cell-lymphom-6
Studies on intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes. II. Adhesive interaction between...Studies on intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes. II. Adhesive interaction between...
J.M. Lackie, P.B. Armstrong; Studies on intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes. II. ... Studies on intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes. II. Adhesive interaction between ... the rabbit peritoneal neutrophil granulocyte) and a host cell type (the chick embryo heart fibroblast) in shaken suspension ...
https://journals.biologists.com/jcs/article/19/3/645/57742/Studies-on-intercellular-invasion-in-vitro-using
THE REQUIREMENTS FOR TRANSFERRIN-DEPENDENT ADHERENCE OF HUMAN-GRANULOCYTES TO POLLEN GRAINS<...THE REQUIREMENTS FOR TRANSFERRIN-DEPENDENT ADHERENCE OF HUMAN-GRANULOCYTES TO POLLEN GRAINS<...
THE REQUIREMENTS FOR TRANSFERRIN-DEPENDENT ADHERENCE OF HUMAN-GRANULOCYTES TO POLLEN GRAINS. In: Allergy. 1986 ; Vol. 41, No. 3 ... THE REQUIREMENTS FOR TRANSFERRIN-DEPENDENT ADHERENCE OF HUMAN-GRANULOCYTES TO POLLEN GRAINS. Allergy. 1986 Apr;41(3):169-178. ... THE REQUIREMENTS FOR TRANSFERRIN-DEPENDENT ADHERENCE OF HUMAN-GRANULOCYTES TO POLLEN GRAINS. / MACKAY, J A ; SASSKUHN, S ; ... title = "THE REQUIREMENTS FOR TRANSFERRIN-DEPENDENT ADHERENCE OF HUMAN-GRANULOCYTES TO POLLEN GRAINS", ...
https://abdn.pure.elsevier.com/en/publications/the-requirements-for-transferrin-dependent-adherence-of-human-gra
CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and...CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and...
Granulocytes generally exert protective roles in the central nervous system (CNS), but recent studies suggest that they can be ... Granulocytes were counted in the brain vasculature after treatment with a neutralizing anti-CXCL1 antibody using stereological ... CXCL1 was the most highly expressed ligand of the granulocyte receptor CXCR2 in the CNS of mice subjected to EAE or infused ... This study identifies CXCL1 not only as a key regulator of granulocyte recruitment into the CNS, but also as a new potential ...
https://listlabs.com/citations_products/cxcl1-can-be-regulated-by-il-6-and-promotes-granulocyte-adhesion-to-brain-capillaries-during-bacterial-toxin-exposure-and-encephalomyelitis/
Development of Simple and Fast Method for Preparation and Purification of Monopegylated Recombinant Human Granulocyte Colony...Development of Simple and Fast Method for Preparation and Purification of Monopegylated Recombinant Human Granulocyte Colony...
PEGylation is a valuable strategy for enhancing the pharmacokinetic properties of recombinant methionyl human granulocyte ... Development of Simple and Fast Method for Preparation and Purification of Monopegylated Recombinant Human Granulocyte Colony- ... "Development of Simple and Fast Method for Preparation and Purification of Monopegylated Recombinant Human Granulocyte Colony- ... Development of Simple and Fast Method for Preparation and Purification of Monopegylated Recombinant Human Granulocyte Colony- ...
http://www.bmmj.org/article_34765.html
Granulocytes - Atlas of Human Anatomy - CentralxGranulocytes - Atlas of Human Anatomy - Centralx
Granulocytes. Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining ...
http://atlas.centralx.com/p/image/hemic-and-immune-systems/immune-system/leukocytes/granulocytes/
Granulocyte and monocyte/macrophage apheresis for the treatment of immune-mediated inflammatory arthropathies: case reports -...Granulocyte and monocyte/macrophage apheresis for the treatment of immune-mediated inflammatory arthropathies: case reports -...
Granulocyte and monocyte/macrophage apheresis for the treatment of immune-mediated inflammatory arthropathies: case reports Ana ... Granulocyte and monocyte/macrophage apheresis (GMA) is a non-pharmacological therapeutic option that is beneficial and very ... Home Articles Granulocyte and monocyte/macrophage apheresis for the treatment of immune-mediated inflammatory arthropathies: ... Carro Martínez AV, Montolio Chiva L, Robustillo Villarino M. Granulocyte and monocyte/macrophage apheresis for the treatment of ...
https://www.drugsincontext.com/granulocyte-and-monocyte-macrophage-apheresis-for-the-treatment-of-immune-mediated-inflammatory-arthropathies-case-reports/
Granulocyte Immunology - ESNEFT PathologyGranulocyte Immunology - ESNEFT Pathology
From 1st September Hologic Aptima collection tubes for Chlamydia and Gonorrhoea no longer accepted. Replaced by Roche 6800 sample collection containers available to order from consumables store. Please follow the link for all details. https://esneftpathology.nhs.uk/wp-content/uploads/2022/08/ESNEFT-Microbiology-Service-Improvement-2022.8.1-1.docx. Read more ...
https://esneftpathology.nhs.uk/test/granulocyte-immunology/
IMSEAR at SEARO: Granulocyte disorders.
Singh H. Granulocyte disorders. Indian Pediatrics. 1985 Apr; 22(4): 259-65. ...
https://imsear.searo.who.int/handle/123456789/6747
MonocytesMacrophageApheresisNeutrophilReduced granulocyte adhesionRecombinant human granulocyteBasophilsNeutrophilic granulocytesEosinophilMyeloid progeDifferentiationNeutropeniaLymphocytesInflammationStimulatesDonorLeukocytesEosinophilicMobilizationMonoclonalPeripheralBasalBoneInfectionBacterialCellsGranulesDoseDonorsTransfusionCytokinePatientsHumanDifferentialCountsInflammatoryInfectionsWhite bloo
Monocytes5
  • Usual care immune parameters used in patients with RA and amino acid serum levels as well as granulocytes and monocytes colony stimulating factor (GM-CSF) serum levels were secondary parameters. (nih.gov)
  • G-CSF is cytokine that acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. (watson-bio.com)
  • The lack of formation of superoxide and hydrogen-peroxide following treatment with unopsonized zymosan indicated that the granulocyte preparation was relatively uncontaminated by monocytes. (cdc.gov)
  • Results and Dialogue Following the exclusion of useless Mitoxantrone distributor cells and/or particles (D) on ahead (FSC) versus (vs.) part scatter (SSC) plots, lymphocytes (L), monocytes (M) and granulocytes (G) had been selected by Compact disc45-APC vs. SSC plots (Fig. 1). (healthweblognews.info)
  • Filstim containing recombinant G-CSF significantly increases the number of neutrophilic granulocytes in the peripheral blood within the first 24 hours after administration and at the same time causes a slight increase in the number of monocytes. (mysalve.com)
Macrophage18
  • Resistance of the body to infection: I. leukocytes, granulocytes, the monocyte-macrophage system, and inflammation. (medlineplus.gov)
  • Granulocyte colony-stimulating factor attenuates liver damage by M2 macrophage polarization and hepatocyte proliferation in alcoholic hepatitis in mice. (harvard.edu)
  • Glycosylation== Human granulocyte macrophage colony-stimulating factor is glycosylated in its mature form. (wikidoc.org)
  • We present the case of a 2-month-old infant with reticular dysgenesis who was treated with recombinant granulocyte-macrophage colony stimulating factor with the aim of stimulating granulopoiesis while awaiting bone marrow transplant. (cun.es)
  • Some mice were given intraperitoneal injections of neutralizing antibodies against granulocyte-macrophage colony-stimulating factor (GM-CSF) or tumor necrosis factor (TNF), or immunoglobulin G1 (control), starting at 1 month of age. (northwestern.edu)
  • Granulocyte and monocyte/macrophage apheresis (GMA) is a non-pharmacological therapeutic option that is beneficial and very well tolerated. (drugsincontext.com)
  • Carro Martínez AV, Montolio Chiva L, Robustillo Villarino M. Granulocyte and monocyte/macrophage apheresis for the treatment of immune-mediated inflammatory arthropathies: case reports. (drugsincontext.com)
  • Granulocyte-Macrophage Progenitor Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (jefferson.edu)
  • The parent cells that give rise to both cells of the GRANULOCYTE lineage and cells of the monocyte/macrophage lineage. (jefferson.edu)
  • This graph shows the total number of publications written about "Granulocyte-Macrophage Progenitor Cells" by people in this website by year, and whether "Granulocyte-Macrophage Progenitor Cells" was a major or minor topic of these publications. (jefferson.edu)
  • Below are the most recent publications written about "Granulocyte-Macrophage Progenitor Cells" by people in Profiles. (jefferson.edu)
  • Human bone marrow mononuclear cells were also cultured in the presence or absence of NE to determine its effects on the proliferation of granulocyte-macrophage colony forming units (CFU-GM). (omeka.net)
  • Treatment of progenitor cells with NE prior to culture inhibited the growth of granulocyte-macrophage colony forming units. (omeka.net)
  • Interleukin-2 (IL-2) and granulocyte-macrophage-colony stimulating factor (GM-CSF) are cytokines with nonoverlapping pleiotropic effects. (elsevier.com)
  • We investigated whether the anti-granulocyte-macrophage-colony stimulating factor (GM-CSF) antibody can suppress microglial activity and decrease Aβ production in Alzheimer's disease transgenic mice (Tg2576 line). (elsevier.com)
  • Humanigen is working with the Department of Defense (DOD) to advance its lenzilumab, an anti-human granulocyte macrophage-colony stimulating factor monoclonal. (scienceboard.net)
  • The current research focuses on typical PKC (cPKC) expression and its regulation in major cultures of bone marrow cells induced to endure macrophage/granulocyte differentiation by macrophage colony-stimulating issue (M-CSF) or granular colony-stimulating issue (G-CSF). (aabioetica.org)
  • The chosen cell line was used safely in cancer vaccination programmes and is well defined (clinical trials utilising the colony-stimulating factor K562 expressing granulocyte-macrophage were used as a tumour vaccine). (pharma-industry-review.com)
Apheresis8
  • To improve the amount and quality of granulocytes (white blood cells) collected by apheresis for donation. (centerwatch.com)
  • Participants will donate granulocytes by apheresis a maximum of 12 times in 1 year. (centerwatch.com)
  • In the 1970-80 s, collection of granulocyte concentrates by apheresis of healthy donors stimulated with corticosteroids alone yielded products with an insufficient number of granulocytes to substantially raise the circulating counts in neutropenic patients. (centerwatch.com)
  • More recently, the ability to give donors recombinant human granulocyte colony-stimulating factor (G-CSF) in combination with corticosteroids (dexamethasone) dramatically increases the circulating neutrophil count prior to apheresis and results in the collection of granulocyte concentrates containing 2 to 6 times as many cells as those collected using steroids alone. (centerwatch.com)
  • Transfusion of daily or every other day granulocytes derived by apheresis of G-CSF and dexamethasone-stimulated donors has been associated in observational and retrospective studies with clearance of life-threatening infections in neutropenic patients, but a single small randomized prospective study did not demonstrate improved survival in neutropenic infected patients who received granulocytes. (centerwatch.com)
  • Clinical Effectiveness of Selective Granulocyte, Monocyte Adsorptive Apheresis with the Adacolumn(®) Device in Ulcerative Colitis. (adacyte.com)
  • The purpose of this meta-analysis was to determine whether selective adsorptive granulocyte and monocyte apheresis (GMA apheresis) using the Adacolumn device can effectively reduce clinical symptoms and endoscopic signs of inflammation in patients with ulcerative colitis (UC). (adacyte.com)
  • Impetigo Herpetiformis Complicated with Intrauterine Growth Restriction Treated Successfully with Granulocyte and Monocyte Apheresis. (nih.gov)
Neutrophil7
  • Studies on intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes. (biologists.com)
  • This hypothesis has been examined by a study of the kinetics of aggregation of dispersed populations of an invasive cell type (the rabbit peritoneal neutrophil granulocyte) and a host cell type (the chick embryo heart fibroblast) in shaken suspension culture. (biologists.com)
  • Granulocytes are typically transfused daily for 5 or more consecutive days and should be continued until infection resolves or absolute neutrophil count remains >500/uL for 48 hours. (medscape.com)
  • Congenital neutrophil defects (i.e., chronic granulomatous disease) and documented refractory bacterial or fungal infection failing to respond to appropriate antimicrobial therapy for more than 24 to 48 hours may be considered for granulocyte transfusion. (medscape.com)
  • Neutrophil and low-density granulocyte (LDG) populations were analyzed by flow cytometry . (bvsalud.org)
  • 1800/μL) is the result of an increase in the marginated granulocyte pool, a portion of which is located in the spleen (see the Absolute Neutrophil Count calculator). (medscape.com)
  • Recombinant human granulocyte colony-stimulating factor is a safe and effective therapeutic modality in management of autoimmune neutropenia associated with Felty's syndrome and systemic lupus erythematosus, which stimulates neutrophil production. (nebraska.edu)
Reduced granulocyte adhesion1
  • The anti-CXCL1 antibody reduced granulocyte adhesion to brain capillaries in the three conditions under study. (listlabs.com)
Recombinant human granulocyte1
  • There is a slight increased risk of exacerbation of the underlying autoimmune disorder, and recombinant human granulocyte colony-stimulating factor dose and frequency should be adjusted at the lowest effective dose. (nebraska.edu)
Basophils1
  • The specific types of granulocytes are neutrophils, eosinophils, and basophils. (medlineplus.gov)
Neutrophilic granulocytes7
  • Human G-CSF regulates the formation of functionally active neutrophilic granulocytes and their entry into the blood from the bone marrow. (mysalve.com)
  • An increase in the number of neutrophilic granulocytes, their functional characteristics depend on the dose. (mysalve.com)
  • In children and adults with severe chronic neutropenia (severe congenital, periodic and malignant neutropenia), the drug stably increases the number of peripheral blood neutrophilic granulocytes and reduces the incidence of infectious complications. (mysalve.com)
  • After treatment with the drug, the number of neutrophilic granulocytes in the peripheral blood decreases by 50% within 1-2 days and returns to normal levels within 1-7 days. (mysalve.com)
  • With prolonged therapy aimed at increasing the number of neutrophilic granulocytes, to reduce the frequency and duration of infectious complications in children and adults with severe congenital, periodic or malignant neutropenia (the absolute number of neutrophilic granulocytes is 500 in 1 mm 3 ) and with severe or recurring infections in the anamnesis. (mysalve.com)
  • Filstim is administered daily until the number of neutrophilic granulocytes reaches the expected minimum, and then normal values. (mysalve.com)
  • Stimulates bactericidal activity of neutrophilic granulocytes, enhancing phagocytosis and increasing unspecific resistance of the organism to infectious diseases. (lekarstwo.ru)
Eosinophil1
  • Eosinophil granulocytes have been implicated in anticancer immunity but recent data indicate that eosinophils can also promote cancer. (regsj.dk)
Myeloid proge1
  • StemSpan™ Myeloid Expansion Supplement (100X) contains a combination of recombinant human cytokines formulated to selectively promote the expansion and differentiation of human myeloid progenitor cells, generating large numbers of CD15+ granulocytes from CD34+ cells isolated from human cord blood (CB) or bone marrow (BM) samples. (stemcell.com)
Differentiation2
  • It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. (harvard.edu)
  • Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. (harvard.edu)
Neutropenia3
  • Outcomes for patients with severe chronic neutropenia treated with granulocyte colony-stimulating factor. (harvard.edu)
  • Any modality which increases the granulocyte count during periods of profound neutropenia and severe infection is thus likely to be of clinical benefit. (centerwatch.com)
  • Background: PEGylation is a valuable strategy for enhancing the pharmacokinetic properties of recombinant methionyl human granulocyte colony-stimulating factor (rh-Met-G-CSF, filgrastim) which is used to treat chemotrapy induced neutropenia. (bmmj.org)
Lymphocytes1
  • The White Blood Count (WBC) in a blood test result is broken down into Granulocytes (GRAN) and Lymphocytes (LYM). (bankruptingamerica.org)
Inflammation3
  • Isolation of basal guinea pig granulocytes for the study of pulmonary inflammation. (cdc.gov)
  • A technique for isolating basal (control) guinea-pig granulocytes for use in studies of pulmonary inflammation was developed. (cdc.gov)
  • The authors conclude that this procedure can isolate purified granulocyte fractions useful for studying the role of granulocytes in pulmonary inflammation. (cdc.gov)
Stimulates2
  • Granulocyte colony-stimulating factor that activates and stimulates production, maturation, migration, and cytotoxicity of neutrophils. (medscape.com)
  • Filgrastim (Granulocyte colony-stimulating factor (G-CSF or GSCF)), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream. (affinityimmuno.com)
Donor5
  • Transfusion of granulocyte concentrates collected after G-CSF and dexamethasone stimulation of the donor typically increases the recipient s granulocyte count by 1,000 cells/uL, and the increase in counts is generally sustained for 24 to 48 hours. (centerwatch.com)
  • The amount of granulocytes per dose can vary greatly (from 1-8 x 1010 granulocytes/collection) based on the donor and mobilization regimen given to the donor. (medscape.com)
  • The volume will vary based on granulocyte concentration of donor but usually ranges from 10-20 mL/kg. (medscape.com)
  • Often times granulocyte donors are friends and family of the recipient because of the commitment needed to be a granulocyte donor. (medscape.com)
  • The donor had scheduled a trip to Sri Lanka, and was to return 3 days before the scheduled start of granulocyte-colony-stimulating factor, or G-CSF, application. (cdc.gov)
Leukocytes1
  • GRANULOCYTES Polymorphonuclear leukocytes (PMNs) Neutrophils: Predominant type of white blood cell. (kupdf.net)
Eosinophilic2
  • Diagnosing eosinophilic asthma using a multivariate prediction model based on blood granulocyte responsiveness. (ox.ac.uk)
  • Despite the absence of unequivocal changes in breathing patterns upon MDI challenge, MDI-sensitized animals displayed elevated anti-MDI immuno- globulin G1 (IgG1) antibodies, and a significant influx of eosinophilic granulocytes in the bronchial wall and lung-associated lymph nodes. (cdc.gov)
Mobilization2
  • To assess the effects of stem cell mobilization following granulocyte colony stimulating factor therapy in patients with acute myocardial infarction. (cochrane.org)
  • Plerixafor, a reversible inhibitor of the binding of stromal cell-derived factor 1 to its cognate receptor CXCR4, has demonstrated a higher capacity for the mobilization of peripheral blood stem cells in combination with granulocyte colony stimulating factor (G-CSF) compared with G-CSF alone. (bvsalud.org)
Monoclonal1
  • Four different monoclonal antibodies (mAbs) reactive with rat CD11b (ED7, ED8, OX-42 and 1B6c) have been characterized for their ability to induce homotypic aggregation of granulocytes or to modify granulocyte adhesiveness triggered by phorbol myristate acetate (PMA) or N-formyl- methionyl-leucyl-phenylalanine (fMLP). (eur.nl)
Peripheral1
  • Background: Immature granulocytes (IG) level in peripheral blood is used as early sign of infection . (bankruptingamerica.org)
Basal1
  • Granulocyte F-(filamentous) actin content between groups was compared, under basal conditions and after cellular stimulation. (duke.edu)
Bone2
  • These cells can be mobilized from the bone marrow by factors such as granulocyte colony stimulating factor (G-CSF). (cochrane.org)
  • The bone marrow creates atypical white blood cells called granulocytes or blasts in people with CML. (medicalnewstoday.com)
Infection4
  • The number of granulocytes in the body usually increases when there is a serious infection. (medlineplus.gov)
  • White blood cells called granulocytes help the body fight infection. (centerwatch.com)
  • Granulocyte transfusion is a supportive adjuvant therapy to manage infections uncontrolled by standard medical therapy and thus should not be used as first line treatment or as the sole treatment of infection. (medscape.com)
  • Granulocytes , which are white blood cells that help fight infection and disease. (cancer.gov)
Bacterial1
  • Granulocytes, specifically neutrophils, help the body fight bacterial infections. (medlineplus.gov)
Cells5
  • These cells could be mobilized to the heart with agents such as granulocyte colony stimulating factor (G-CSF). (cochrane.org)
  • When added to serum-free medium, StemSpan™ Myeloid Expansion Supplement typically promotes the production of thousands of CD15+ granulocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. (stemcell.com)
  • Granulocyte products typically contain a large amount of red blood cells and thus should be ABO and Rh compatible and require RBC crossmatching to be issued. (medscape.com)
  • We analyzed whether granulocyte-colony stimulating factor (G-CSF), widely used in adjuvant cancer therapy to alleviate chemotherapy-induced myelotoxicity, could influence ErbB2 expression in BC cells and patients. (elsevier.com)
  • 6 Granulocyte colony-stimulating factor (G-CSF) mobilizes CD34+ cells and improves the survival of ACLF patients. (ejohg.com)
Granules1
  • Granulocytes are a type of white blood cell that has small granules. (medlineplus.gov)
Dose1
  • Studies at the NIH Department of Transfusion Medicine (DTM) have defined the optimal timing and dose of these drugs in granulocyte donors, and these components have been used for clinical care since 1996. (centerwatch.com)
Donors2
  • Granulocyte components are not recognized as a licensed blood component by the Food and Drug Administration (FDA), and neither G-CSF nor dexamethasone is approved by the FDA for use in allogeneic granulocytapheresis donors. (centerwatch.com)
  • Granulocyte donors are almost always given medication regimens to increase the circulating granulocyte counts to improve collection. (medscape.com)
Transfusion2
  • Please read entire monograph if you are considering granulocyte transfusion. (medscape.com)
  • Decision to transfuse granulocytes should be made in consultation with the transfusion medicine physician because of a lack of established efficacy in randomized controlled trials and the coordination required to provide granulocytes as they must be collected the day of transfusion. (medscape.com)
Cytokine1
  • Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine. (watson-bio.com)
Patients2
  • Although granulocytes may be important in the pathogenesis of diabetic retinopathy, granulocyte deformability (measured by mean entry time) and F-actin content are not significantly different between healthy patients and those with diabetes. (duke.edu)
  • METHODS: Clinical parameters, activation of blood granulocytes and sputum characteristics were assessed in 115 adult asthma patients (training cohort/Utrecht) and 34 patients (validation cohort/Oxford). (ox.ac.uk)
Human2
  • Promotes the production of thousands of CD15+ granulocytes per input CD34+ human CB cell in 14-day liquid cultures. (stemcell.com)
  • It is produced by a genetically modified culture of Escherichia coli BL21 (DE3) / pES3-7 containing the gene for human granulocyte colony stimulating factor (G-CSF). (mysalve.com)
Differential1
  • Granulocytes are counted as part of a white blood cell differential test . (medlineplus.gov)
Counts1
  • Doctors may conduct blood cell counts to check for abnormally high granulocyte levels. (medicalnewstoday.com)
Inflammatory1
  • The purpose of the experiments was to develop an animal model suitable for examining the role of granulocytes in organic dust toxic syndrome and other pulmonary inflammatory disorders. (cdc.gov)
Infections1
  • People with a lower number of granulocytes are more likely to develop bad infections more often. (medlineplus.gov)
White bloo1
  • And I bring that up because I kind of like to understand what words mean, and a granulocyte is a type of white blood cell. (cdc.gov)
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