Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.
A genus of aerobic, gram-negative, motile, slightly curved, rod-shaped bacteria. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
Cyclopentanophenanthrenes with a 5- or 6-membered lactone ring attached at the 17-position and SUGARS attached at the 3-position. Plants they come from have long been used in congestive heart failure. They increase the force of cardiac contraction without significantly affecting other parameters, but are very toxic at larger doses. Their mechanism of action usually involves inhibition of the NA(+)-K(+)-EXCHANGING ATPASE and they are often used in cell biological studies for that purpose.
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
Polysaccharides consisting of xylose units.
A genus of gram-negative, anaerobic bacteria in the family Fibrobacteraceae, isolated from the human GASTROINTESTINAL TRACT.
A genus of gram-negative, straight or curved rods which are motile by means of a single, polar flagellum. Members of this genus are found in coastal waters and the open ocean. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
A genus of gram-positive bacteria in the family Lachnospiraceae that inhabits the RUMEN; LARGE INTESTINE; and CECUM of MAMMALS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process of cleaving a chemical compound by the addition of a molecule of water.
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
A genus of fungi in the family Neocallimasticaceae, order NEOCALLIMASTIGALES. They contain polyflagellate zoospores and grow on a range of simple and complex carbohydrates in the rumen of sheep and cattle.
A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.
The relationships of groups of organisms as reflected by their genetic makeup.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A mitosporic fungal genus frequently found in soil and on wood. It is sometimes used for controlling pathogenic fungi. Its teleomorph is HYPOCREA.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
Proteins found in any species of bacterium.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
Enzymes that catalyze reversibly the formation of an epoxide or arene oxide from a glycol or aromatic diol, respectively.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
Glycosides from plants of the genus DIGITALIS. Some of these are useful as cardiotonic and anti-arrhythmia agents. Included also are semi-synthetic derivatives of the naturally occurring glycosides. The term has sometimes been used more broadly to include all CARDIAC GLYCOSIDES, but here is restricted to those related to Digitalis.
The rate dynamics in chemical or physical systems.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
A subclass of iridoid compounds that include a glycoside moiety, usually found at the C-1 position.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Enzymes which catalyze the hydrolysis of carboxylic acid esters with the formation of an alcohol and a carboxylic acid anion.
A cardiac glycoside sometimes used in place of DIGOXIN. It has a longer half-life than digoxin; toxic effects, which are similar to those of digoxin, are longer lasting. (From Martindale, The Extra Pharmacopoeia, 30th ed, p665)
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
The genetic complement of a BACTERIA as represented in its DNA.
A type of MONOTERPENES, derived from geraniol. They have the general form of cyclopentanopyran, but in some cases, one of the rings is broken as in the case of secoiridoid. They are different from the similarly named iridals (TRITERPENES).
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The above-ground plant without the roots.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Proteins prepared by recombinant DNA technology.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A mass spectrometric technique that is used for the analysis of a wide range of biomolecules, such as glycoalkaloids, glycoproteins, polysaccharides, and peptides. Positive and negative fast atom bombardment spectra are recorded on a mass spectrometer fitted with an atom gun with xenon as the customary beam. The mass spectra obtained contain molecular weight recognition as well as sequence information.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
C(23)-steroids with methyl groups at C-10 and C-13 and a five-membered lactone at C-17. They are aglycone constituents of CARDIAC GLYCOSIDES and must have at least one double bond in the molecule. The class includes cardadienolides and cardatrienolides. Members include DIGITOXIN and DIGOXIN and their derivatives and the STROPHANTHINS.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
A group of 3-hydroxy-4-keto-FLAVONOIDS.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2.
A subclass of iridoid compounds that include a glucoside moiety, usually found at the C-1 position.
Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.
Saturated derivatives of the steroid pregnane. The 5-beta series includes PROGESTERONE and related hormones; the 5-alpha series includes forms generally excreted in the urine.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.
A plant genus of the family RANUNCULACEAE that contains triterpenoid saponins. Remifemin from C. racemosa is used to suppress LUTEINIZING HORMONE. It is reclassified by some to ACTAEA. The common name of black snakeroot is also used with ASARUM and SANICULA.
A flavonol glycoside found in many plants, including BUCKWHEAT; TOBACCO; FORSYTHIA; HYDRANGEA; VIOLA, etc. It has been used therapeutically to decrease capillary fragility.
Phosphoric acid esters of mannose.
A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.
An autolytic enzyme bound to the surface of bacterial cell walls. It catalyzes the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell wall glycopeptides, particularly peptidoglycan. EC 3.5.1.28.
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
Root-like underground horizontal stem of plants that produces shoots above and roots below. Distinguished from true roots which don't have buds and nodes. Similar to true roots in being underground and thickened by storage deposits.
A beta-N-Acetylhexosaminidase that catalyzes the hydrolysis of terminal, non-reducing 2-acetamido-2-deoxy-beta-glucose residues in chitobiose and higher analogs as well as in glycoproteins. Has been used widely in structural studies on bacterial cell walls and in the study of diseases such as MUCOLIPIDOSIS and various inflammatory disorders of muscle and connective tissue.
An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.
The buttercup plant family of the order Ranunculales, subclass Magnoliidae, class Magnoliopsida. The leaves are usually alternate and stalkless. The flowers usually have two to five free sepals and may be radially symmetrical or irregular.
A plant genus of the family ASTERACEAE. Members contain stevioside and other sweet diterpene glycosides. The leaf is used for sweetening (SWEETENING AGENTS).
A group of inherited metabolic diseases characterized by the accumulation of excessive amounts of acid mucopolysaccharides, sphingolipids, and/or glycolipids in visceral and mesenchymal cells. Abnormal amounts of sphingolipids or glycolipids are present in neural tissue. INTELLECTUAL DISABILITY and skeletal changes, most notably dysostosis multiplex, occur frequently. (From Joynt, Clinical Neurology, 1992, Ch56, pp36-7)
A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose.
Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.
A receptor that is specific for IGF-II and mannose-6-phosphate. The receptor is a 250-kDa single chain polypeptide which is unrelated in structure to the type 1 IGF receptor (RECEPTOR, IGF TYPE 1) and does not have a tyrosine kinase domain.
A group of FLAVONOLS based on kaempferol. They are derived from naringenin and can be hydroxylated to QUERCETIN or reduced to leucopelargonidin.
Cholestane derivatives containing a fused lactone ring at the 16,17-position and a spiroglycosidic linkage at C-22. Members include sarsaponin, DIOSGENIN and yamogenin.
A hexosaminidase specific for non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. It acts on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES. Two specific mammalian isoenzymes of beta-N-acetylhexoaminidase are referred to as HEXOSAMINIDASE A and HEXOSAMINIDASE B. Deficiency of the type A isoenzyme causes TAY-SACHS DISEASE, while deficiency of both A and B isozymes causes SANDHOFF DISEASE. The enzyme has also been used as a tumor marker to distinguish between malignant and benign disease.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
A flavonol widely distributed in plants. It is an antioxidant, like many other phenolic heterocyclic compounds. Glycosylated forms include RUTIN and quercetrin.
A plant genus of the family ASCLEPIADACEAE. This is the true milkweed; APOCYNUM & EUPHORBIA hirta are rarely called milkweed. Asclepias asthmatica has been changed to TYLOPHORA.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.
An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.
The characteristic 3-dimensional shape of a carbohydrate.
A group of DITERPENES cyclized into four rings.
A group of FLAVONOIDS characterized with a 4-ketone.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
The figwort plant family of the order Lamiales. The family is characterized by bisexual flowers with tubular corollas (fused petals) that are bilaterally symmetrical (two-lips) and have four stamens in most, two of which are usually shorter.
Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.
A group of enzymes that catalyze the hydrolysis of diphosphate bonds in compounds such as nucleoside di- and tri-phosphates, and sulfonyl-containing anhydrides such as adenylylsulfate. (Enzyme Nomenclature, 1992) EC 3.6.
An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.
Enzymes that catalyze the hydrolysis of a phenol sulfate to yield a phenol and sulfate. Arylsulfatase A, B, and C have been separated. A deficiency of arylsulfatases is one of the causes of metachromatic leukodystrophy (LEUKODYSTROPHY, METACHROMATIC). EC 3.1.6.1.
Thirteen-carbon butene cyclohexene degradation products formed by the cleavage of CAROTENOIDS. They contribute to the flavor of some FRUIT. Ionone should not be confused with the similarly named ionol.
A group of phenyl benzopyrans named for having structures like FLAVONES.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
A plant genus of the family CRASSULACEAE. Members contain rhodioloside. This roseroot is unrelated to the familiar rose (ROSA). Some species in this genus are called stonecrop which is also a common name for SEDUM.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A group of lysosomal proteinases or endopeptidases found in aqueous extracts of a variety of animal tissues. They function optimally within an acidic pH range. The cathepsins occur as a variety of enzyme subtypes including SERINE PROTEASES; ASPARTIC PROTEINASES; and CYSTEINE PROTEASES.
Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.
Chinese herbal or plant extracts which are used as drugs to treat diseases or promote general well-being. The concept does not include synthesized compounds manufactured in China.
The characteristic three-dimensional shape of a molecule.
A class of enzymes involved in the hydrolysis of the N-glycosidic bond of nitrogen-linked sugars.
An intracellular proteinase found in a variety of tissue. It has specificity similar to but narrower than that of pepsin A. The enzyme is involved in catabolism of cartilage and connective tissue. EC 3.4.23.5. (Formerly EC 3.4.4.23).
A genus of fungi in the family Neocallimasticaceae, order NEOCALLIMASTICALES, containing uniflagellate zoospores.
The mint plant family. They are characteristically aromatic, and many of them are cultivated for their oils. Most have square stems, opposite leaves, and two-lipped, open-mouthed, tubular corollas (united petals), with five-lobed, bell-like calyxes (united sepals).
A plant family of the order Lamiales. It is characterized by simple leaves in opposite pairs, cystoliths (enlarged cells containing crystals of calcium carbonate), and bilaterally symmetrical and bisexual flowers that are usually crowded together. The common name for Ruellia of wild petunia is easily confused with PETUNIA.
An enzyme that catalyzes the conversion of acetate esters and water to alcohols and acetate. EC 3.1.1.6.
A plant genus of the family OROBANCHACEAE. Members contain phenylethanoid glycosides.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An organophosphate insecticide that inhibits monoamine oxidase and acetylcholinesterase. It has been shown to be genotoxic.
A class of enzymes that transfers substituted phosphate groups. EC 2.7.8.
The morning glory family of flowering plants, of the order Solanales, which includes about 50 genera and at least 1,400 species. Leaves are alternate and flowers are funnel-shaped. Most are twining and erect herbs, with a few woody vines, trees, and shrubs.
Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The Madder plant family of the order Rubiales, subclass Asteridae, class Magnoliopsida includes important medicinal plants that provide QUININE; IPECAC; and COFFEE. They have opposite leaves and interpetiolar stipules.
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.
Flammable, amorphous, vegetable products of secretion or disintegration, usually formed in special cavities of plants. They are generally insoluble in water and soluble in alcohol, carbon tetrachloride, ether, or volatile oils. They are fusible and have a conchoidal fracture. They are the oxidation or polymerization products of the terpenes, and are mixtures of aromatic acids and esters. Most are soft and sticky, but harden after exposure to cold. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)
A plant genus of the family RUBIACEAE. Members contain genepin, from which geniposide is obtained for use as a crosslinking agent in ADHESIVES, and 3-caffeoyl-4-sinapoylquinic acid.
An endocellulase with specificity for the hydrolysis of 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans including laminarin, paramylon, and pachyman.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
A plant genus of the family CORNACEAE. It is widely cultivated for the attractive flowers.
A plant genus of the family MYRTACEAE that bears an edible fruit and contains guavin B and quercetin glycosides.
A plant genus of the family ASCLEPIADACEAE. Members contain steroidal glycosides and cytotoxic phenanthroindolizidine N-oxide alkaloids.
Polyacenes with four ortho-fused benzene rings in a straight linear arrangement. This group is best known for the subclass called TETRACYCLINES.

N-Linked glycosylation and sialylation of the acid-labile subunit. Role in complex formation with insulin-like growth factor (IGF)-binding protein-3 and the IGFs. (1/4049)

Over 75% of the circulating insulin-like growth factors (IGF-I and -II) are bound in 140-kDa ternary complexes with IGF-binding protein-3 (IGFBP-3) and the 84-86-kDa acid-labile subunit (ALS), a glycoprotein containing 20 kDa of carbohydrate. The ternary complexes regulate IGF availability to the tissues. Since interactions of glycoproteins can be influenced by their glycan moieties, this study aimed to determine the role of ALS glycosylation in ternary complex formation. Complete deglycosylation abolished the ability of ALS to associate with IGFBP-3. To examine this further, seven recombinant ALS mutants each lacking one of the seven glycan attachment sites were expressed in CHO cells. All the mutants bound IGFBP-3, demonstrating that this interaction is not dependent on any single glycan chain. Enzymatic desialylation of ALS caused a shift in isoelectric point from 4.5 toward 7, demonstrating a substantial contribution of anionic charge by sialic acid. Ionic interactions are known to be involved in the association between ALS and IGFBP-3. Desialylation reduced the affinity of ALS for IGFBP-3. IGF complexes by 50-80%. Since serum protein glycosylation is often modified in disease states, the dependence of IGF ternary complex formation on the glycosylation state of ALS suggests a novel mechanism for regulation of IGF bioavailability.  (+info)

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. (2/4049)

Mild alkaline treatment of glycopeptide (GP-II) resulted in the loss of 1 mole of serine and 5 moles of threonine per mole of GP-II, suggesting the presence of O-glycosyl bonds between 1 serine and 5 threonine residues and carbohydrate chains. Treatment of GP-II with alkaline borohydride released only disaccharide. Methylation studies of the carbohydrate moiety gave 2,3,4,6-tetra-O-methyl and 2,4,6-tri-O-methyl derivatives of mannose in a ratio of approximately 1:1. In addition, one step of Smith degradation resulted in the loss of about 6 residues of mannose per mole of GP-II. Moreover, alpha-mannosidase [EC 3.2.1.24] liberated about 6 residles of mannose per mole of GP-II. On the basis of these data, the structure of the carbohydrate moiety of GP-II was confirmed to be 3-O-alpha-mannosylmannose. The amino- and carboxyl-terminal amino acids of GP-II were determined to be threonine and serine, respectively. On reductive cleavage of N-proline bonds with metallic sodium in liquid ammonia, 2 moles of alanine per mole of GP-II were lost. From the compositions of three fragments isolated from the reductive cleavage products, the amino acid sequence of the peptide portion of GP-II was determined. Based on these data, a probable structure was proposed for GP-II.  (+info)

Relationship between glycosyl hydrolase inventory and growth physiology of the hyperthermophile Pyrococcus furiosus on carbohydrate-based media. (3/4049)

Utilization of a range of carbohydrates for growth by the hyperthermophile Pyrococcus furiosus was investigated by examining the spectrum of glycosyl hydrolases produced by this microorganism and the thermal labilities of various saccharides. Previously, P. furiosus had been found to grow in batch cultures on several alpha-linked carbohydrates and cellobiose but not on glucose or other beta-linked sugars. Although P. furiosus was not able to grow on any nonglucan carbohydrate or any form of cellulose in this study (growth on oat spelt arabinoxylan was attributed to glucan contamination of this substrate), significant growth at 98 degrees C occurred on beta-1,3- and beta-1,3-beta-1,4-linked glucans. Oligosaccharides generated by digestion with a recombinant laminarinase derived from P. furiosus were the compounds that were most effective in stimulating growth of the microorganism. In several cases, periodic addition of beta-glucan substrates to fed-batch cultures limited adverse thermochemical modifications of the carbohydrates (i.e., Maillard reactions and caramelization) and led to significant increases (as much as two- to threefold) in the cell yields. While glucose had only a marginally positive effect on growth in batch culture, the final cell densities nearly tripled when glucose was added by the fed-batch procedure. Nonenzymatic browning reactions were found to be significant at 98 degrees C for saccharides with degrees of polymerization (DP) ranging from 1 to 6; glucose was the most labile compound on a mass basis and the least labile compound on a molar basis. This suggests that for DP of 2 or greater protection of the nonreducing monosaccharide component may be a factor in substrate availability. For P. furiosus, carbohydrate utilization patterns were found to reflect the distribution of the glycosyl hydrolases which are known to be produced by this microorganism.  (+info)

Rapid identification of Actinomycetaceae and related bacteria. (4/4049)

Identification of new isolates belonging to the family Actinomycetaceae requires extensive numbers of biochemical tests, supplemented with gas-liquid chromatography determination of fermentation end products and, often, analysis of cell wall composition. This paper describes the results of the testing of 162 strains of Actinomycetaceae and related taxa for 20 different enzymatic activities including phosphatases, esterases, aminopeptidases, and glycosidases. The results of all tests were read after 4 h of incubation. The results obtained in the study provide significant new information on the biochemical properties of these groups of bacteria. An identification scheme based upon 13 selected tests, which allow the identification of these groups of bacteria within 4 h, is proposed.  (+info)

Purification and characterization of Aspergillus ficuum endoinulinase. (5/4049)

Endoinulinase from Aspergillus ficuum, which catalyzes the hydrolysis of inulin via an endo-cleavage mode, was purified by chromatography from Novozym 230 as a starting commercial enzyme mixture on CM-Sephadex and DEAE-Sepharose, and by preparative electrophoresis under native conditions. The enzyme was estimated to be pure on the basis of its I/S ratio, whose value was infinite in our assay conditions. Two forms separated by using this method. SDS gel electrophoresis showed the two purified forms to respectively exhibit molecular weights of 64,000 +/- 500 and 66,000 +/- 1,000. The results of deglycosylation indicated that the two forms were originally the same protein but with different sugar contents. A molecular weight of 54,800 +/- 1,500 was found by gel filtration of the native enzyme, indicating the native functional protein to be a monomer. The enzyme showed nearly absolute substrate specificity towards inulin and inulooligosaccharides, and acted via an endo-attack to produce mainly inulotriose during the late stage of the reaction. The apparent Km and Vmax values for inulin hydrolysis were 8.1 +/- 1.0 mM and 773 +/- 60 U/mg, respectively. The internal peptides of the enzyme showed sequence homology to the endoinulinase of Penicillium purpurogenum.  (+info)

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus nidulans amdS-lacZ fusion gene system. (6/4049)

Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved sequences, designated Regions I, II, and III, compared with promoter regions of the A. oryzae glaA encoding glucoamylase and the agdA encoding alpha-glucosidase. To identify the function of these sequences within the amyB promoter, various fragments containing conserved sequences in the amyB promoter were introduced into the upstream region of the heterologous A. nidulans amdS gene (encoding acetamidase) fused to the Escherichia coli lacZ gene as a reporter. Introduction of the sequence between -290 to -233 (the number indicates the distance in base pairs from the translation initiation point (+1)) containing Region III significantly increased the expression of the lacZ reporter gene in the presence of maltose. The sequence between -377 to -290 containing Region I also increased the lacZ activity, but its maltose inducibility was less than that of Region III. The sequence between -233 to -181 containing Region II had no effect on the expression. These results indicated that Region III is most likely involved in the maltose induction of the amyB gene expression.  (+info)

A single limit dextrinase gene is expressed both in the developing endosperm and in germinated grains of barley. (7/4049)

The single gene encoding limit dextrinase (pullulan 6-glucanohydrolase; EC 3.2.1.41) in barley (Hordeum vulgare) has 26 introns that range in size from 93 to 822 base pairs. The mature polypeptide encoded by the gene has 884 amino acid residues and a calculated molecular mass of 97,417 D. Limit dextrinase mRNA is abundant in gibberellic acid-treated aleurone layers and in germinated grain. Gibberellic acid response elements were found in the promoter region of the gene. These observations suggest that the enzyme participates in starch hydrolysis during endosperm mobilization in germinated grain. The mRNA encoding the enzyme is present at lower levels in the developing endosperm of immature grain, a location consistent with a role for limit dextrinase in starch synthesis. Enzyme activity was also detected in developing grain. The limit dextrinase has a presequence typical of transit peptides that target nascent polypeptides to amyloplasts, but this would not be expected to direct secretion of the mature enzyme from aleurone cells in germinated grain. It remains to be discovered how the enzyme is released from the aleurone and whether another enzyme, possibly of the isoamylase group, might be equally important for starch hydrolysis in germinated grain.  (+info)

Characterization of the divergent sacBK and sacAR operons, involved in sucrose utilization by Lactococcus lactis. (8/4049)

The divergently transcribed sacBK and sacAR operons, which are involved in the utilization of sucrose by Lactococcus lactis NZ9800, were examined by transcriptional and gene inactivation studies. Northern analyses of RNA isolated from cells grown at the expense of different carbon sources revealed three sucrose-inducible transcripts: one of 3.2 kb containing sacB and sacK, a second of 3.4 kb containing sacA and sacR, and a third of 1.8 kb containing only sacR. The inactivation of the sacR gene by replacement recombination resulted in the constitutive transcription of the sacBK and sacAR operons in the presence of different carbon sources, indicating that SacR acts as a repressor of transcription.  (+info)

Glycoside hydrolases (O-Glycosyl hydrolases) EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of numerous different families. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in clans. As of October 2011, CAZy includes 128 families of glycosyl hydrolases and 14 clans. Glycoside hydrolase family 1 Glycoside hydrolase family 2 Glycoside hydrolase family 3 Glycoside hydrolase family 4 Glycoside hydrolase family 5 Glycoside hydrolase family 6 Glycoside hydrolase family 7 Glycoside hydrolase family 8 Glycoside hydrolase family 9 Glycoside hydrolase family 10 Glycoside hydrolase family 11 Glycoside hydrolase family 12 Glycoside ...
Carbohydrase is an enzyme that catalyzes the breakdown of carbohydrates into simple sugars. Carbohydrases are produced in the pancreas but act in the stomach breaking down carbohydrates, hence the name. Carbohydrate is usually a compound consisting of carbon, hydrogen and oxygen. Carbohydrase is a protein/enzyme that catalyses carbohydrates to break down the compound Carbohydrate so the products can be simple sugar which is a Monosaccharide. Maltase reduces maltose into glucose: C12H22O11 + H2O → 2C6H12O6Maltose + Water → α-Glucose http://www.bbc.co.uk/schools/gcsebitesize/science/add_aqa_pre_2011/enzymes/enzymes_and_digestion4. ...
Food enzymes act as highly selective catalyst and function in the metabolic reactions and in the inter conversion of complex molecules to smaller ones. Enzymes form an integral part of the global food additives industry. These are primarily involved in conversion of carbohydrate to carbohydrase, protein to protease, and lipid to lipase. Food enzymes such as carbohydrase, protease, and lipase have anti-staling, digestive, and softening properties, which increase the shelf life of foods. The advent of many such versatile functions has escalated their usage in application-specific ingredients and new upscale end-use products.
article{9b276fde-3141-41ff-ba06-611da1a392ac, abstract = {,p,Lipases and glycoside hydrolases have large similarities concerning reaction mechanisms. Acyl-enzyme intermediates are formed during lipase-catalyzed reactions and in an analogous way, retaining glycoside hydrolases form glycosyl-enzyme intermediates during catalysis. In both cases, the covalent enzyme intermediates can react with water or other nucleophiles containing hydroxyl groups. Simple alcohols are accepted as nucleophiles by both types of enzymes. Lipases are used very successfully in synthesis applications due to their efficiency in catalyzing reversed hydrolysis and transesterification reactions. On the other hand, synthesis applications of glycoside hydrolases are much less developed. Here, important similarities and differences between the enzyme groups are reviewed and approaches to reach high synthesis yields are discussed. Useful strategies include the use of low-water media, high nucleophile concentrations, as well as ...
A major challenge for the pig industry is to formulate starter diets that primarily fit the digestive capacity, maintain GIT health and promote growth without recourse to in-feed antimicrobials. Experiments were conducted to evaluate the efficacy of carbohydrase enzymes (CE) targeting non-starch polysaccharides (NSP) in enhancing gut health and function in piglets. First, an experiment was conducted to evaluate the effects of adding CE in piglet diets on growth performance, GIT bacterial activity and nutrient digestibility. Pigs fed diets containing CE had a higher ileal lactobacilli count, total organic acids concentrations, NSP digestibility and low ammonia compared with control. The effectiveness of CE targeting NSP was further evaluated using enterotoxigenic E. coli (ETEC) in a challenge model to evaluate the impact on gut health and function. Two approaches for the ETEC challenge were adopted; an in situ small intestine segments perfusion model and an in vivo model. Initially, a pilot study ...
Genome sequencing of Catenovulum agarivorans YM01T reveals 15 open-reading frames (ORFs) encoding various agarases. In this study, extracellular proteins of YM01T were precipitated by ammonium sulfate and separated by one-dimensional gel electrophoresis. The results of in-gel agarase activity assay and mass spectrometry analysis revealed that the protein, YM01-3, was an agarase with the most evident agarolytic activity. Agarase YM01-3, encoded by the YM01-3 gene, consisted of 420 amino acids with a calculated molecular mass of 46.9 kDa and contained a glycoside hydrolase family 16 β-agarase module followed by a RICIN superfamily in the C-terminal region. The YM01-3 gene was cloned and expressed in Escherichia coli. The recombinant agarase, YM01-3, showed optimum activity at pH 6.0 and 60 °C and had a Km of 3.78 mg mL−1 for agarose and a Vmax of 1.14 × 104 U mg−1. YM01-3 hydrolyzed the β-1,4-glycosidic linkages of agarose, yielding neoagarotetraose and neoagarohexaose as the main products.
Using a combination of comparison algorithms the glycoside hydrolases have been classified into more than 100 GH families [2]. This classification is permanently available through the Carbohydrate Active enZyme database [4]. Classification of glycoside hydrolases into families allows many useful predictions to be made since it has long been noted that the catalytic machinery and molecular mechanism is conserved for the vast majority of the GH families [6] as well as the geometry around the glycosidic bond (irrespective of naming conventions) [7]. Usually, the mechanism used (ie retaining or inverting) is conserved within a GH family. One notable exception is the glycoside hydrolases of family GH97, which contains both retaining and inverting enzymes; a glutamate acts as a general base in inverting members, whereas an aspartate likely acts as a catalytic nucleophile in retaining members [8]. Another mechanistic curiosity are the glycoside hydrolases of familes GH4 and GH109 which operate through ...
As a rule, about 1% of genes in a given genome encode glycoside hydrolases and their homologues. On the basis of sequence similarity they have been grouped into more than ninety GH families during the last 15 years. The GH97 family has been established very recently and initially included only 18 bacterial proteins. However, the evolutionary relationship of the genes encoding proteins of this family remains unclear, as well as their distribution among main groups of the living organisms. The extensive search of the current databases allowed us to double the number of GH97 family proteins. Five subfamilies were distinguished on the basis of pairwise sequence comparison and phylogenetic analysis. Iterative sequence analysis revealed the relationship of the GH97 family with the GH27, GH31, and GH36 families of glycosidases, which belong to the α-galactosidase superfamily, as well as a more distant relationship with some other glycosidase families (GH13 and GH20). The results of this study show an
Some glycoside hydrolases have broad specificity for hydrolysis of glycosidic bonds, potentially increasing their functional utility and flexibility in physiological and industrial applications. To deepen the understanding of the structural and evolutionary driving forces underlying specificity patterns in glycoside hydrolase family 5, we quantitatively screened the activity of the catalytic core domains from subfamily 4 (GH5_4) and closely related enzymes on four substrates: lichenan, xylan, mannan, and xyloglucan. Phylogenetic analysis revealed that GH5_4 consists of three major clades, and one of these clades, referred to here as clade 3, displayed average specific activities of 4.2 and 1.2U/mg on lichenan and xylan, approximately 1 order of magnitude larger than the average for active enzymes in clades 1 and 2. Enzymes in clade 3 also more consistently met assay detection thresholds for reaction with all four substrates. We also identified a subfamily-wide positive correlation between ...
TY - JOUR. T1 - Gaining insight into the inhibition of glycoside hydrolase family 20 exo-β-N-acetylhexosaminidases using a structural approach.. AU - Sumida, Tomomi. AU - Stubbs, Keith A.. AU - Ito, Makoto. AU - Yokoyama, Shigeyuki. PY - 2012/4/7. Y1 - 2012/4/7. N2 - One useful methodology that has been used to give insight into how chemically synthesized inhibitors bind to enzymes and the reasons underlying their potency is crystallographic studies of inhibitor-enzyme complexes. Presented here is the X-ray structural analysis of a representative family 20 exo-β-N-acetylhexosaminidase in complex with various known classes of inhibitor of these types of enzymes, which highlights how different inhibitor classes can inhibit the same enzyme. This study will aid in the future development of inhibitors of not only exo-β-N-acetylhexosaminidases but also other types of glycoside hydrolases.. AB - One useful methodology that has been used to give insight into how chemically synthesized inhibitors bind ...
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The global market for enzymes is expected to reach USD 7,652.0 million by 2020, according to a new study by Grand View Research, Inc. Increased demand for ...
Robb, CS, Mystkowska, AA and Hehemann, JH (2017) Crystal structure of a marine glycoside hydrolase family 99-related protein lacking catalytic machinery. Protein Science, 26(12). 2445-2450. doi:10.1002/pro.3291 ...
Content is to be added here. Authors may get an idea of what to put in each field from Curator Approved Glycoside Hydrolase Families. (TIP: Right click with your mouse and open this link in a new browser window...) In the meantime, please see these references for an essential introduction to the CAZy classification system: [1, 2]. ...
糖質に対して活性をもつ酵素群 (Carbohydrate-Active enZymes, CAZymes) の構造と機能に関する研究は,ここ10年ほどの間に大幅な発展を遂げてきた.特に,糖質加水分解酵素 (Glycoside Hydrolase, GH) ファミリーは100を超え,その立体構造 (フォールド) は非常にバラエティに富んでいることが明らかになってきている.このレヴューでは,われわれのグループが新規に構造を決定した4種類のGHファミリー (GH42, GH57, GH54, GH94) の立体構造を中心に,一見無関係にみえるGHファミリー間でみつかった構造と反応機構の類似点から,それらの進化的な関連の可能性について議論する.. Studies of the structure and function of Carbohydrate-Active enZymes (CAZymes) have made a great deal of progress over the last decade. The glycoside hydrolase (GH) family is a prominent class of CAZymes. There are more than 100 GH families, with wide ...
Phenyl Glycofuranosides. 1. Synthesis of Phenyl beta-D-Xylofuranoside and Phenyl alpha-L-Arabinofuranoside.. B rjeson, Hans; Jerkeman, Per; Lindberg, Bengt ...
Marine bacterium Catenovulum agarivorans YM01(T) can produce highly thermostable agarases. The draft genome of YM01(T) is about 5.36 Mb and harbors approximately 4,913 genes, including 15 agarase (2 α-agarase and 13 β-agarase)-encoding genes, which will provide references to functional characterization of various agarases from marine bacteria.
Global Agricultural Enzymes Market. WiseGuyRerports.com Presents Global Agricultural Enzymes Market 2017-2021 New Document to its Studies Database. The Report Contain 93 Pages With Detailed Analysis.. Description. Agricultural enzymes are complex protein molecules that are generally derived from sources such as plant tissues, animal tissues, and microbes. They are known to play a major role in preserving the land, water, and biodiversity. Enzymes accelerate chemical reactions and catalyze biochemical reactions that do not occur naturally. Agricultural enzymes are categorized into carbohydrases, proteases, lipases, and others. They are used in fertility, growth-enhancing, and control products.. The analysts forecast the global agricultural enzymes market to grow at a CAGR of 7.36% during the period 2017-2021.. Covered in this report ...
Glycoside Hydrolase Family 31, 978-620-0-10221-8, Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In molecular biology, glycoside hydrolase family 31 is a family of glycoside hydrolases. Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of |100 different families. This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site, and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.
article{dd239673-ae87-4b21-bd30-83e6d058111e, abstract = {In mature seeds of Brassica napus three major and three minor myrosinase isoenzymes were identified earlier. These myrosinases are known to be encoded by at least two different families of myrosinase genes, denoted MA and MB. In the work described in this paper the presence of different myrosinase isoenzymes in embryos, seedlings, and vegetative mature tissues of B. napus was studied and related to the expression of myrosinase MA and MB genes in the same tissues to facilitate future functional studies of these enzymes. In developing seeds, myrosinases of 75, 73, 70, 68, 66, and 65 kD were present. During seedling development there was a turnover of the myrosinase pool such that in 5-d-old seedlings the 75-, 70-, 66-, and 65-kD myrosinases were present, with the 70- and 75-kD myrosinases predominating. In 21-d-old seedlings the same myrosinases were present, but the 66- and 65-kD myrosinase species were most abundant. At flowering the ...
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glycoside hydrolase family 2 protein; FUNCTIONS IN: carbohydrate binding, cation binding, beta-galactosidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds, catalytic activity; INVOLVED IN: carbohydrate metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: male gametophyte, guard cell; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase family 2, immunoglobulin-like beta-sandwich (InterPro:IPR006102), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, family 42, domain 5 (InterPro:IPR004199), Glycoside hydrolase family 2, TIM barrel (InterPro:IPR006103), Glycoside hydrolase, family 2 (InterPro:IPR006101), Glycoside hydrolase-type carbohydrate-binding (InterPro:IPR011013), Glycoside hydrolase-type carbohydrate-binding, subgroup (InterPro:IPR014718), Glycoside hydrolase family 2, carbohydrate-binding (InterPro:IPR006104), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycoside hydrolase, families 2 and 20, immunoglobulin-like ...
An enzyme is a protein molecule that catalyzes the rate of a chemical reaction. Enzymes are obtained from micro-organisms, plants, animals, etc. and aid in conversion of reactants into products. Industrial enzymes are primarily used in food processing, alcohol production, manufacturing of chemicals used for cleaning of fabrics, etc. On the basis of functionality, industrial enzymes are categorized into carbohydrases, proteases, lipases, and others. Among these, the demand for carbohydrases is growing at the fastest pace in India as they are widely used in various industries such as baking, brewing, dairy, etc. Increasing demand for packaged food, dairy products, detergents and pharmaceuticals that aid in digestion is driving the countrys industrial enzymes market. Moreover, an increasing number of detergent manufacturersare shifting their focus towards adopting enzymes for detergent formulations due to environmental concerns associated with the usage of phosphorus in detergents.. According to ...
Analysis of the Global Weather Forecasting Equipment Market. The presented global Weather Forecasting Equipment market report provides reliable and credible insights related to the various segments and sub-segments of the market. The market study throws light on the various factors that are projected to impact the overall dynamics of the global Weather Forecasting Equipment market over the forecast period (20XX-20XX).. According to the report, the value of the Weather Forecasting Equipment market was estimated to reach ~US$ XX in 2019 and attain a market value of ~US$ XX by the end of 2029. Further, the study reveals that the market is set to grow at a CAGR of XX% during the forecast period owing to a plethora of factors.. Exciting offers for first-time buyers!. Make An Enquiry About This Report @ https://www.marketresearchhub.com/enquiry.php?type=E&repid=2532441&source=atm The market study aims to provide answers to the following questions related to the Weather Forecasting Equipment ...
Purchase high Purity Wheat Arabinoxylan (enzyme debranched 30% Ara) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
A. tumefaciens is used in the construction of genetically engineered plants, as it is able to transfer DNA to plant hosts. Knowledge of the mechanisms of DNA transfer and the genes required will aid in the understanding of this process. Manipulation of glycoside hydrolases may increase transformation and widen the host range of the bacterium.... ...
The recent years have witnessed considerable developments in the interpretation of the three-dimensional structures of plant polysaccharide-degrading enzymes in the context of their functional specificity. A plethora of new structures of catalytic, carbohydrate-binding and protein-scaffolding modules involved in (hemi)cellulose catabolism has emerged in harness with sophisticated biochemical analysis. Despite significant advances, a full understanding of the intricacies of substrate recognition and catalysis by these diverse and specialised enzymes remains an important goal, especially if the application potential of these biocatalysts is to be fully realised.. ...
Purchase high purity dyed crosslinked Xylazyme AF for the measurement of enzyme activity for research, enzyme assays and in vitro diagnostic analysis.
Glycosidase activities in principal cells, basal cells, fibroblasts and spermatozoa isolated from the rat epididymis.: The activity levels of beta-N-acetylgluco
Gaucher disease (GD) is the most common lysosomal disorder and is caused by an inherited autosomal recessive deficiency in β-glucocerebrosidase. This enzyme, like other glycohydrolases involved in glycosphingolipid (GSL) metabolism, is present in bo
O-Glycosyl hydrolases ( EC 3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ (PUBMED:7624375) (PUBMED:8535779) ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website. Glycoside hydrolase family 31 comprises enzymes with several known activities; alpha-glucosidase ( EC 3.2.1.20 ), alpha-galactosidase ( EC 3.2.1.22 ); glucoamylase ( EC 3.2.1.3 ), sucrase-isomaltase ( EC 3.2.1.48 ); isomaltase ( EC 3.2.1.10 ); alpha-xylosidase ( EC 3.2.1 ); alpha-glucan lyase ( EC 4.2.2.13 ). Glycoside hydrolase family 31 groups a number of glycosyl hydrolases on the basis of sequence similarities [ (PUBMED:1747104) (PUBMED:1761061) (PUBMED:1743281) ]. An aspartic acid has been implicated [ (PUBMED:1856189) ] in the catalytic activity of ...
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Carbohydrate-binding modules (CBMs) are found within multi-modular polysaccharide degrading enzymes [glycoside hydrolases (GHs)]. CBMs play a critical role in the recognition of plant cell-wall polysaccharides and enhance the hydrolase activity of their cognate catalytic domains by increasing enzyme substrate proximity. Mimicking their role in Nature, we, in the present study, propose that CBMs may assist in vitro glycosynthase-catalysed polymerization reactions to produce artificial polysaccharides. Glycosynthases are GHs that have been engineered to catalyse glycoside bond formation for the synthesis of oligosaccharides, glycoconjugates and glycans. The degree of polymerization (DP) of the glycans generated is limited by the solubility of the polymeric product. In the present study, we have targeted the synthesis of artificial 1,3-1,4-β-glucans with a regular sequence using the glycosynthase E134S derived from a Bacillus licheniformis lichenase. We show that the addition of CBM11, which binds ...
glycanase: catalyzes hydrolysis of beta-D-glucosido-(1-73)-D-glucuronic acid link in capsular glycan of Klebsiella bacteriophage No. 11.
This tissue pattern shows the pancreatic duct ( the central hole) surrounded by a cuboidal epithelium. The pancreas produces and sets free digestive enzymes into the bile duct, through the pancreatic duct. Pancreatic enzymes are being classified into: lipase: which decay lipids carbohydrases: which decay sugars and starch nuclease: which decay nucleic acids
With an interest to enhance the aroma of palm kernel oil (PKO), Viscozyme L, an enzyme complex containing a wide range of carbohydrases, was applied to alter the carbohydrates in palm kernels (PK) to modulate the formation of volatiles upon kernel roasting. After Viscozyme treatment, the content of simple sugars and free amino acids in PK increased by 4.4-fold and 4.5-fold, respectively. After ker ...
Sow performance has increased in recent years. Improvements in genetics, nutrition and health status have driven these changes. Some nutritional solutions to deal with the increased demands for sows are still underutilised, carbohydrases being one of them.
The focus of our Carbohydrate Enzymology Research Group is to understand the way in which particular enzymes act to alter the structure of polysaccharides found in biomass (especially plant cell walls and wood fibers), and to harness these enzymes for applications. We are primarily concerned with the carbohydrate-active enzymes (CAZymes) which synthesize (eg., glycosyl transferases, transglycosidases) or degrade (eg., glycoside hydrolases) glycosidic bonds. Fundamental studies and applications of these enzymes are especially topical for improving the use of renewable biomass resources in the biofuels/bioenergy, wood fiber, textile, agricultural, and food industries. ...
The focus of our Carbohydrate Enzymology Research Group is to understand the way in which particular enzymes act to alter the structure of polysaccharides found in biomass (especially plant cell walls and wood fibers), and to harness these enzymes for applications. We are primarily concerned with the carbohydrate-active enzymes (CAZymes) which synthesize (eg., glycosyl transferases, transglycosidases) or degrade (eg., glycoside hydrolases) glycosidic bonds. Fundamental studies and applications of these enzymes are especially topical for improving the use of renewable biomass resources in the biofuels/bioenergy, wood fiber, textile, agricultural, and food industries. ...
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Many amounts of microorganisms are grown in the industry to be used as enzymes which are passed out of the cell. They are grown relatively cheaply and quickly in vats, fermenters, and no expensive equipment is needed. The fermenters are kept at 27° C to keep cost low.. Enzymes are used as catylists in enzyme technology. They are efficient as only small amounts are required. They are also used in the cooking industry in baby foods, as they are pre-digested foods, making the food softer and easier for the babies to digest.. The can also be used to sweeten foods, this is done by breaking done starch to sugar syrup using carbohydrase to catalyse the reaction. Isomerase is an enzymme used to convert glucose syrup to fructose syrup which is sweeter than glucose so less needs to be added to foods, this is useful for slimming foods and low calorie drinks.. ...
the proteins in baby food are pre-digested using protein-digesting enzymes (protease) so they become easier to digest. Carbohydrate-digesting enzymes (carbohydrase) can be used to turn starch syrup into sugar syrup. Glucose syrup can be turned into fructose syrup using isomerase, fructose is sweeter so less is needed ...
One litter of 12 piglets was used in a completely random design to evaluate the effects of hydrocortisone acetate injections on organ weight and carbohydrase activities. Dams milk was the only food source available to the ...
Formerly known as glycosyltransferase family GT36; Assigned to a GH family following a paper by Hidaka, Honda, Kitaoka, Nirasawa, Hayashi, Wakagi, Shoun and Fushinobu (Structure (Camb). 2004 Jun;12(6):937-947) (PMID: 15274915) that revealed the evolutionary, structural and mechanistic relationship of these phosphorylases with glycoside hydrolases of clan GH-L ...
Formerly known as glycosyltransferase family GT36; Assigned to a GH family following a paper by Hidaka, Honda, Kitaoka, Nirasawa, Hayashi, Wakagi, Shoun and Fushinobu (Structure (Camb). 2004 Jun;12(6):937-947) (PMID: 15274915) that revealed the evolutionary, structural and mechanistic relationship of these phosphorylases with glycoside hydrolases of clan GH-L ...
The microbial degradation of the plant cell wall is a pivotal biological process that is of increasing industrial significance. One of the major plant structural polysaccharides is mannan, a beta-1,4-linked d-mannose polymer, which is hydrolyzed by endo- and exo-acting mannanases. The mechanisms by which the exo-acting enzymes target the chain ends of mannan and how galactose decorations influence activity are poorly understood. Here we report the crystal structure and biochemical properties of CjMan26C, a Cellvibrio japonicus GH26 mannanase. The exo-acting enzyme releases the disaccharide mannobiose from the nonreducing end of mannan and mannooligosaccharides, harnessing four mannose-binding subsites extending from -2 to +2. The structure of CjMan26C is very similar to that of the endo-acting C. japonicus mannanase CjMan26A. The exo-activity displayed by CjMan26C, however, reflects a subtle change in surface topography in which a four-residue extension of surface loop creates a steric block at ...
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β-Mannosidases are exo-acting glycoside hydrolases (GHs) that catalyse the removal of the nonreducing end β-D-mannose from manno-oligosaccharides or mannoside-substituted molecules. They play important roles in fundamental biological processes and also have potential applications in various industries. In this study, the first fungal GH family 5 β-mannosidase (RmMan5B) from Rhizomucor miehei was functionally and structurally characterized. RmMan5B exhibited a much higher activity against manno-oligosaccharides than against p-nitrophenyl β-D-mannopyranoside (pNPM) and had a transglycosylation activity which transferred mannosyl residues to sugars such as fructose. To investigate its substrate specificity and transglycosylation activity, crystal structures of RmMan5B and of its inactive E202A mutant in complex with mannobiose, mannotriose and mannosyl-fructose were determined at resolutions of 1.3, 2.6, 2.0 and 2.4 Å, respectively. In addition, the crystal structure of R. miehei β-mannanase (RmMan5A
TY - JOUR. T1 - PARG activity mediates intestinal injury induced by splanchnic artery occlusion and reperfusion. AU - Cuzzocrea, Salvatore. AU - Di Paola, Rosanna. AU - Mazzon, Emanuela. AU - Cortes, Ulrich. AU - Genovese, Tiziana. AU - Muià, Carmelo. AU - Li, Weixing. AU - Xu, Weizheng. AU - Li, Jia He. AU - Zhang, Jie. AU - Wang, Zhao Qi. PY - 2005/4. Y1 - 2005/4. N2 - Poly (ABP-ribosyl)ation, an early post-translational modification in response to DNA damage, is catalyzed by poly (ADP-ribose) polymerase (PARP-1) and caiabolized by poly(ABP-ribose) glycohydrolase (PARG). The aim of this study was to investigate the role of PARG on the modulation of the inflammatory response caused by splanchnic ischemia and reperfusion. SAO shock in rats and wild-type (WT) mice was associated with a significant neutrophil infiltration in the ileum and production of tumor necrosis factor-α (TNF-α). Reperfused ileum tissue sections from SAO-shocked WT mice and rats showed positive staining for P-selectin and ...
The predominant characteristic feature of C. pinensis is the ability to degrade chitin, a β-1,4-glycosidic linked homopolymer of N-acetyl-D-glucosamine and one of the most abundant polysaccharides in nature. It is a component of fungal cell walls and of arthropod exoskeletons. Chitin is degraded by chitinases (EC 3.2.1.14); endochitinases randomly cleave within the chitin molecule and exochitinases hydrolyze diacetylchitobiose from the end of a chitin chain. Diacetylchitobiose is further degraded to N-acetylglucosamine by the action of N-acetylglucosaminidases (EC 3.2.1.52).. These glycosidic bond hydrolyzing enzymes were grouped in glycoside hydrolase (GH) families based on amino acid sequence similarities (Web Site) [22]. For the C. pinensis genome 169 glycoside hydrolases belonging to 49 different GH families are predicted; 18 of the predicted glycoside hydrolases belong to GH family 43 which contains xylosidases, xylanases, arabinanases, arabinofuranosidases and galactosidases. Because of ...
Hemicellulase is used to clarify the juice of mandarin oranges. Although pectinase is used to clarify the juices of apples and grapes, the juice of mandarin oranges are clarified by the simultaneous use of pectinase and hemicellulase. In food processing, it is rare that the desired result can be obtained with the use of hemicellulase alone. Hemicellulases work in conjunction with cellulases and pectinases ...
Buy high purity recombinant enzyme endo-1-4-beta-Mannanase Cellvibrio japonicus for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Caldicellulosiruptor glycoside hydrolase inventory.As mentioned above, only two (C. bescii and C. saccharolyticus) of the nine described Caldicellulosiruptor species currently have publically available genome sequences (29, 51). Due to renewed interest in biomass-degrading microbes, five additional genome sequencing projects are under way, three of which are finished (for C. hydrothermalis, C. kronotsyensis, and C. owensensis), to help pinpoint what makes some species more cellulolytic than others in the genus. Draft and finished versions of these genome sequences were examined for GH family diversity.. Previous studies looking into Caldicellulosiruptor diversity have identified glycoside hydrolases, mostly cellulases and xylanases, produced by selected species (4, 19). Here, the biomass deconstruction-related GH inventory, inferred from each draft and finished genome sequence, was examined to provide insights into the observed differential responses to microcrystalline cellulose. Based on the ...
Molecular Dynamics Studies of Biomass Degradation in Biofuel Production. We are developing a joint computational and experimental approach where we will take advantage of molecular modeling and dynamics tools, together with some of the fastest supercomputers available, to tackle two major problems of the second-generation biofuel industry, namely: the product inhibition and the effects of pretreatment with EBI developed polymers on hydrolysis of cellulose fibers by glycoside hydrolases. In the first, in collaboration with Dr. Doug Clarks group, we plan to address the problem of product inhibition of glycoside hydrolases, by studying mutants identified by the experimental group. In the second, in collaboration with Dr. Matt Franciss group, we will model the effects of a synthetic polymer on the cellulose fiber and also the interaction of this polymer with enzymes of the glycoside hydrolase family.. ...
Arabinoxylan gels exhibiting different rheological and lycopene transport properties were obtained by modifying the polysaccharide concentration from 3 to 4% (w/v). The apparent lycopene diffusion coefficient decreased from 2.7 × 10−7 to 2.4 × 10−7 cm2/s as the arabinoxylan concentration in the gel changed from 3 to 4% (w/v). A low amount of lycopene is released by diffusion from arabinoxylan gels. These results indicate that arabinoxylan gels could be carriers for lycopene delivery in specific sites after network degradation. The possibility to modulate lycopene release from arabinoxylan gels makes these biomaterials potential candidates for the controlled delivery of biomolecules.
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A series of commercially available carbohydrases as well as a freeze dried aqueous malt extract were examined for their specific activity and for stability after pasteurisation. It was found that most of the enzymes (except malt extract) retained their activity after pasteurisation. The changes occurring in the pattern of some carbohydrates in wort and beer brought about by the presence of these enzymes in the fermenter was also examined. It was found that each enzyme preparation characteristically changed the carbohydrate spectrum in the wort and even minute amounts of the freeze dried aqueous malt extract added to the fermenter increased the final attenuation. The use of these enzymes in the fermenter makes it possible to use materials as kettle adjuncts which contain relatively small amounts of fermentable matter.
These results have direct application to production and therefore pork producers will find this information to be valuable as they make management decisions that affect productivity and profitability. The webinar will be presented by the principle investigator from each project and webinar participants will have the opportunity to interact with the investigator following the presentation.. August 1, 12:00 P.M., CST: Dr. Dean Boyd - How Dietary Enzymes Work. View Summary View Webinar. August 8, 12:00 P.M., CST: Dr. Pedro Urriola - Gut Physiology of Pigs Fed Diets with Carbohydrases Enzymes. View Summary View Webinar. August 15, 12:00 P.M., CST: Dr. Eric van Heugten - Applying Enzyme Technology to Optimize the Utilization of Fibrous Feed Ingredients. View Webinar. August 22, 12:00 P.M., CST: Dr. Merlin Lindemann - Evaluation of the Nutrient Uplift Provided by Xylanase in Finishing Diets. View Summary View Webinar. ...
Chitosanase is able to specifically cleave β-1,4-glycosidic bond linkages in chitosan to produce a chito-oligomer product, which has found a variety of applications in many areas, including functional food and cancer therapy. Although several structures for chitosanase have been determined, the substrate-binding mechanism for this enzyme has not been fully elucidated because of the lack of a high-resolution structure of the chitosanase-substrate complex. In the present study we show the crystal structure of a novel chitosanase OU01 from Microbacterium sp. in complex with its substrate hexa-glucosamine (GlcN)6, which belongs to the GH46 (glycoside hydrolyase 46) family in the Carbohydrate Active Enzymes database (http://www.cazy.org/). This structure allows precise determination of the substrate-binding mechanism for the first time. The chitosanase-(GlcN)6 complex structure demonstrates that, from the −2 to +1 position of the (GlcN)6 substrate, the pyranose rings form extensive interactions ...
α-L-Arabinofuranosidases play an important role in the degradation of hemicellulosic and pectinaceous plant polysaccharides. Reflecting their importance, arabinofuranosidases are found in several distinct phylogenetic lineages, including glycoside hydrolase (GH) families 43, 51, 54, and 62. Though many α-L-arabinofuranosidase structures have been solved, there remained a significant gap in our understanding of GH51 enzymes; no structure of an industrially relevant fungal GH51 enzyme was known. A lack of sequence similarity between fungal and bacterial enzymes precluded any inference of key function-determining structural features. Of particular interest is the fact that, during the degradation of arabinoxylan, some fungal GH51 enzymes show weak activity towards doubly substituted xylose residues, while others do not. Missing activity towards doubly substituted positions necessitates the inclusion of another enzyme to fully degrade this substrate. Structural understanding of a fungal GH51 ...
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Please choose your preferred method of contact. Registered mail or parcel services offering tracking. Nutri D-DAY - Premium Fish Collagen 3-Month Set $ US 49.90. 14 enzymes pour une digestion facile. NOUVEAUTÉS. HERBS is a Hong Kong brand founded in 1998. NOUVEAUTÉS. 3) 薬剤師からのQpostにご返信ください。 Find calories, carbs, and nutritional contents for umeya and over 2,000,000 other foods at MyFitnessPal.com. 0. One piece per day can aid you regular bowel movements, detoxifications and a healthy life with golden feces. ALL PRODUCTS Read more. A carbohydrase can be used to convert starch syrup into sugar syrup. 該当の注文番号のご注文がキャンセルとなります。 Its function is helping to reduce the absorption rate of fat, sugar, and carbohydrate while people having meals. Please consult your doctor if you have any problems. PHY276 - Enzymup 120 gélules > Par mail > FAQ. Add more items and get a discount. Cashback Reward: US$0.97. Cet article est une liste ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Poly(ADP-ribose) polymerase 1 (PARP-1) and poly(ADP-ribose) glycohydrolase (PARG) regulate transcriptional activity by modifying target nuclear proteins with the addition and removal of ADP-ribose polymers, respectively. While the role of PARP-1 has been established, the exact function of PARG in the nucleus is less clear, although its assumed that PARP-1 and PARG have opposing functions in gene regulation. In this article, however, the authors show that this is not the case. Combining short hairpin RNA (shRNA) knockdown with microarray analysis in MCF-7 cells, they determined that these two enzymes often act in a similar, rather than antagonistic, manner. PARP-1 and PARG generally localized to similar target promoters, most notably in genes for stress response and metabolism, and, in about half of the genes tested, PARP-1 binding was dependent on PARG. In addition, studies using shRNA-resistant catalytic mutants revealed that enzymatic activity was not required in some target genes. So, rather ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
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Endo-β-d-glucosaminidases from family 85 of glycoside hydrolases (GH85 endohexosaminidases) act to cleave the glycosidic linkage between...
The IUPHAR/BPS Guide to Pharmacology. amylase, alpha 2A (pancreatic) - 3.2.1.- Glycosidases. Detailed annotation on the structure, function, physiology, pharmacology and clinical relevance of drug targets.
"Glycoside hydrolases". CAZypedia. Retrieved 2021-04-30. Frandsen TP, Svensson B (May 1998). "Plant alpha-glucosidases of the ... glycoside hydrolase family 31. Molecular properties, substrate specificity, reaction mechanism, and comparison with family ...
However, all three isoforms exhibit glycoside hydrolase activity. Protein O-GlcNAcases belong to glycoside hydrolase family 84 ... Protein O-GlcNAcase (EC 3.2.1.169, OGA, glycoside hydrolase O-GlcNAcase, O-GlcNAcase, BtGH84, O-GlcNAc hydrolase) is an enzyme ... "Glycoside Hydrolase Family 84". Cazypedia. Retrieved 28 March 2017. Gao Y, Wells L, Comer FI, Parker GJ, Hart GW (March 2001 ... with systematic name (protein)-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase. OGA is encoded ...
Henrissat B, Davies GJ (December 2000). "Glycoside hydrolases and glycosyltransferases. Families, modules, and implications for ... The domain is also found in an Olive pollen allergen as well as at the C terminus of family 17 glycosyl hydrolases. Simpson C, ...
Thus, glycoside hydrolases are gaining great interest in biorefining applications in the future bioeconomy. Some examples are ... Another important group of thermostable enzymes are glycoside hydrolases. These enzymes are responsible of the degradation of ... Linares-Pastén, J. A.; Andersson, M; Nordberg karlsson, E (2014). "Thermostable glycoside hydrolases in biorefinery ... hence give thermostable variants of glycoside hydrolases an important role in this context. Protein engineering can be used to ...
Glycoside hydrolases are the enzymes involved in the degradation of the major fraction of biomass, such as polysaccharides ... Linares-Pastén, J. A.; Andersson, M; Nordberg karlsson, E (2014). "Thermostable glycoside hydrolases in biorefinery ...
"Glycoside Hydrolase Family 61". CAZypedia.org. Retrieved 2018-03-06. CAZypedia Consortium (December 2018). "Ten years of ... The family was previously incorrectly classified as glycoside hydrolase family 61, however it was re-classified in March 2013. ...
Henrissat, B.; Davies, G. (1997). "Structural and sequence-based classification of glycoside hydrolases". Current Opinion in ... Davies, G.; Henrissat, B. (1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853-9. doi:10.1016/ ...
Glycoside hydrolases catalyze the breakage of glycosidic bonds. They are used to modify the oligosaccharide structure of the ... normally due to a defect in a glycoside hydrolase enzyme. Sphingolipidoses are typically inherited, and their effects depend on ...
... inhibits glycoside hydrolases, specifically N-linked glycosylation. Disruption of Golgi alpha-mannosidase II with ...
Henrissat, B.; Davies, G. (1997). "Structural and sequence-based classification of glycoside hydrolases". Current Opinion in ... Included in the database are families of glycoside hydrolases, glycosyltransferases, polysaccharide lyases, carbohydrate ... Current statistics are available on each Glycoside Hydrolase, Glycosyltransferase, Polysaccharide Lyase, Carbohydrate Esterase ... which was originally developed in early 1990s to classify the glycoside hydrolases. New entries are added shortly after they ...
It is a member of glycoside hydrolase family 13. Although found in many tissues, amylase is most prominent in pancreatic juice ... Fleming D, Chahin L, Rumbaugh K (February 2017). "Glycoside Hydrolases Degrade Polymicrobial Bacterial Biofilms in Wounds". ...
"Glycoside Hydrolase Family 13 - CAZypedia". www.cazypedia.org. Retrieved 2021-03-06. Nichols BL, Avery S, Sen P, Swallow DM, ... Maltase is part of a group of intestinal enzymes called FamilyGH13 (Glycoside hydrolase family 13) that are responsible for ... Fifty years later entering the genomic age, cloning and sequencing of the mucosal starch hydrolase confirmed Dahlqvist and ... alpha-glucoside hydrolase, alpha-1,4-glucosidase, alpha-D-glucoside glucohydrolase) is one type of alpha-glucosidase enzymes ...
This enzyme belongs to GH (glycoside hydrolases) family 94. The systematic name of this enzyme class is 1,4-beta-D-oligo-D- ...
This enzyme is part of glycoside hydrolase family 16. Xyloglucan endo-transglycosylase (XET) is thought to be highly important ...
Glycoside hydrolases (or glycosidases), are enzymes that break glycosidic bonds. Glycoside hydrolases typically can act either ... N-glycosides), or even -CR1R2R3 (C-glycosides). Particularly in naturally occurring glycosides, the compound ROH from which the ... A substance containing a glycosidic bond is a glycoside. The term 'glycoside' is now extended to also cover compounds with ... "glycoside hydrolases" are among the most common catalysis. The former often needs expensive materials and the later often shows ...
doi:10.1016/0003-9861(90)90346-Z. Henrissat, B.; Davies, J. G. (2000). "Glycoside Hydrolases and Glycosyltransferases: Families ... A member of the glycoside hydrolase family, myrosinase possesses several similarities with the more ubiquitous O-glycosidases. ...
... such as glycoside hydrolase family 15 in fungi, glycoside hydrolase family 31 of human MGAM, and glycoside hydrolase family 97 ... They belong to glycoside hydrolase family 14. Both α-amylase and β-amylase are present in seeds; β-amylase is present in an ... They belong to glycoside hydrolase family 13. Because it can act anywhere on the substrate, α-amylase tends to be faster-acting ... All amylases are glycoside hydrolases and act on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1 ) (CAS 9014-71-5) ( ...
March 2010). "Crystal structure of a glycoside hydrolase family 6 enzyme, CcCel6C, a cellulase constitutively produced by ... "Glycoside Hydrolases Degrade Polymicrobial Bacterial Biofilms in Wounds". Antimicrobial Agents and Chemotherapy. 61 (2): AAC. ... "Engineering of family-5 glycoside hydrolase (Cel5A) from an uncultured bacterium for efficient hydrolysis of cellulosic ... "Characterization of all family-9 glycoside hydrolases synthesized by the cellulosome-producing bacterium Clostridium ...
... but forms a β 1-3 glycoside with Xylose as the acceptor. Glucosidase Glycoside hydrolase family 1 PDB entry 1ODZ Hancock, S. M ... The first glycosynthase was a retaining exoglycosidase that catalyzed the formation of β 1-4 linked glycosides of glucose and ... The enzymes underwent a transglycosidation reaction to form a disarccharide, which was then a substrate for hydrolase activity ... β-glucosythase, which forms a β 1-4 glycoside with Glucose as the acceptor, ...
Enzymes that hydrolyze glycosidic bonds are called "glycoside hydrolases" or "glycosidases". The best-known disaccharide is ...
Glycopeptide α-N-acetylgalactosaminidases belong to family GH101 of glycoside hydrolases. Ashida H, Maki R, Ozawa H, Tani Y, ... Naumoff DG (June 2010). "GH101 family of glycoside hydrolases: subfamily structure and evolutionary connections with other ... "Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-alpha-N- ... crystallization and preliminary X-ray diffraction analysis of a family 101 glycoside hydrolase from Streptococcus pneumoniae". ...
... is a glycoside hydrolase that catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl- ... ISBN 978-0-8493-8935-1. Williams S, Vocadlo D. "Glycoside hydrolase family 22". Cazypedia. Retrieved 11 April 2017. Yoshimura K ... making them part of the same glycoside hydrolase family 22. In humans, the C-type lysozyme enzyme is encoded by the LYZ gene. ... Glycoside hydrolysis by lysozyme and beta-glucosidase". Biochemistry. 8 (10): 4214-21. doi:10.1021/bi00838a045. PMID 5388150. ...
These flagellate protists, including Trichonympha, convert cellulose into sugar using glycoside hydrolases. The sugar is then ...
Its neuraminidase domain has the CAZy designation glycoside hydrolase family 83 (GH83). It does show a structural similarity to ...
β-glucosidases belong to GH3, another glycoside hydrolase family. C. hutchinsonii contains four β-glucosidases located in ... which are known glycoside hydrolase families. Eight of the genes coding for endoglucanases are cel5A, cel5B, cel5C, cel9A, ...
... such as glycoside hydrolase family 15 in fungi, glycoside hydrolase family 31 of human MGAM, and glycoside hydrolase family 97 ... They belong to glycoside hydrolase family 14. Both α-amylase and β-amylase are present in seeds; β-amylase is present in an ... They belong to glycoside hydrolase family 13. Because it can act anywhere on the substrate, α-amylase tends to be faster-acting ... All amylases are glycoside hydrolases and act on α-1,4-glycosidic bonds. ...
... , and other structurally-related iminosugars, inhibit glycoside hydrolase enzymes called alpha-glucosidases. Since ...
... are enzymes (glycoside hydrolases) that catalyze the hydrolysis of galactosides into monosaccharides. ...
"Glycoside hydrolase, family 1, beta-glucosidase (IPR017736) < InterPro < EMBL-EBI". www.ebi.ac.uk. Retrieved 2015-12-11. ... β-galactosidase, also called lactase, beta-gal or β-gal, is a family of glycoside hydrolase enzymes that catalyzes the ... and lactase-phlorizin hydrolase. Although they may be structurally similar, they all have different functions. Beta-gal is ... which refers to the hydrolases. β-gal belongs to a sub-class of glycosylases with an oxygen substrate nature. β-galactosidase ...
His fundamental includes investigation of structure-activity relationships of enzymes, (particularly glycoside hydrolases and ... 2006). "Understanding the polymerization mechanism of glycoside-hydrolase family 70 glucansucrases". Journal of Biological ... 2013). "Role of glycoside phosphorylases in mannose foraging by human gut bacteria". The Journal of Biological Chemistry. 288 ( ...
Hydrolase: sugar hydrolases (EC 3.2). 3.2.1: Glycoside hydrolases. Disaccharidase. *Sucrase/Sucrase-isomaltase/Invertase ... hydrolase activity, acting on glycosyl bonds. • maltose alpha-glucosidase activity. • catalytic activity. • hydrolase activity ...
Hydrolase: sugar hydrolases (EC 3.2). 3.2.1: Glycoside hydrolases. Disaccharidase. *Sucrase/Sucrase-isomaltase/Invertase ...
"Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases". Proc. ...
Enzymes that hydrolyse glycosidic bonds are called "glycoside hydrolases" or "glycosidases". The best-known disaccharide is ... The glycoside bond is represented by the central oxygen atom, which holds the two monosaccharide units together. ...
glycoside metabolic process. • aromatic amino acid family metabolic process. • response to lipopolysaccharide. • cerebral ... EC3 Hydrolases (list). *EC4 Lyases (list). *EC5 Isomerases (list). *EC6 Ligases (list) ...
... is a cyanogenic glycoside related to amygdalin. Chemically, it is the glucoside of (R)-mandelonitrile. ... "Prunasin Hydrolases during Fruit Development in Sweet and Bitter Almonds". Plant Physiology. 158 (4): 1916-32. doi:10.1104/pp. ... Peter R. Cheeke (1989). Toxicants of Plant Origin: Glycosides. 2. CRC Press. p. 137.. ...
The sweet taste of the fruit comes mainly from mogrosides, a group of triterpene glycosides that make up about 1% of the flesh ... epoxide hydrolases, cytochrome P450s, and UDP-glucosyltransferases.[6] The metabolic pathway for mogroside biosynthesis ... The sweetness of the fruit is increased by the mogrosides, a group of triterpene glycosides (saponins). The five different ...
Glycoside hydrolases are classified into EC 3.2.1 as enzymes catalyzing the hydrolysis of O- or S-glycosides. Glycoside ... Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) catalyze the hydrolysis of glycosidic bonds in complex ... Mutant glycoside hydrolases termed glycosynthases have been developed that can achieve the synthesis of glycosides in high ... "Glycoside hydrolase" - news · newspapers · books · scholar · JSTOR (December 2010) (Learn how and when to remove this template ...
"Cyanogenic glycosides - Information Sheet" (PDF). New Zealand Food Safety Authority. 2006. Archived (PDF) from the original on ... "Prunasin hydrolases during fruit development in sweet and bitter almonds". Plant Physiology. 158 (4): 1916-32. doi:10.1104/pp. ...
hydrolase activity. • ATP binding. • xenobiotic-transporting ATPase activity. • phosphatidylethanolamine-translocating ATPase ... Cardiac glycosides like digoxin. *Immunosuppressive agents. *Glucocorticoids like dexamethasone. *HIV-type 1 antiretroviral ...
"Cyanogenic glycosides - Information Sheet" (PDF). New Zealand Food Safety Authority. 2006. Archived (PDF) from the original on ... "Prunasin hydrolases during fruit development in sweet and bitter almonds". Plant Physiology. 158 (4): 1916-32. doi:10.1104/pp. ... The fruit of the wild forms contains the glycoside amygdalin, "which becomes transformed into deadly prussic acid (hydrogen ...
... by sucrase or isomaltase glycoside hydrolases, which are located in the membrane of the microvilli lining the duodenum.[41][42] ...
Glycoside hydrolases are the enzymes involved in the degradation of the major fraction of biomass, such as polysaccharides ... "Thermostable glycoside hydrolases in biorefinery technologies" (PDF). Current Biotechnology. 3 (1): 26-44. doi:10.2174/ ...
Aryldialkylphosphatase (also known as organophosphorus hydrolase, phosphotriesterase, and paraoxon hydrolase) uses an aryl ... a known bitter-tasting flavonoid glycoside, can be isolated from the rhizomes of Selliguea feei.[57] Hypogallic acid, caffeic ... which means that the natural phenols are mostly found in the glycoside form instead of the aglycone form. ...
McCarter JD, Withers SG (decembar 1994). "Mechanisms of enzymatic glycoside hydrolysis". Curr. Opin. Struct. Biol. 4 (6): 885- ...
Hydrolase (EC 3). *3.1 Phosphodiesterase. *Acetylcholinesterase. *Ribonuclease. *3.2 Polygalacturonase. *Neuraminidase. *Alpha- ...
"A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a ... Itoh, T., Ochiai, A., Mikami, B., Hashimoto, W. and Murata, K. (2006). "Structure of unsaturated rhamnogalacturonyl hydrolase ...
More specifically it has been placed in the retaining glycoside hydrolases family although it catalyzes a transglycosidation ...
... enzymes are glycoside hydrolase enzymes that cleave the glycosidic linkages of neuraminic acids. Neuraminidase ...
... a glycoside called salicin, was first isolated by Johann Andreas Buchner in 1827. By 1829, French chemist Henri Leroux had ... enhance endocannabinoid signaling by blocking the anandamide-degrading membrane enzyme fatty acid amide hydrolase (FAAH).[78] ... "A binding site for nonsteroidal anti-inflammatory drugs in fatty acid amide hydrolase". J Am Chem Soc. 135 (1): 22-25. doi ...
Sucrases and invertases are types of glycoside hydrolase enzymes. Acid, such as lemon juice or cream of tartar, can also ...
Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases». Proc. ...
In glycolysis, oligo- and polysaccharides are cleaved first to smaller monosaccharides by enzymes called glycoside hydrolases. ...
Hydrolase: sugar hydrolases (EC 3.2). 3.2.1: Glycoside hydrolases. Disaccharidase. *Sucrase/Sucrase-isomaltase/Invertase ...
... s, an aa kent as muramidase or N-acetylmuramide glycanhydrolase, are glycoside hydrolases. These are enzymes (EC 3.2. ...
Glycoside hydrolase enzymes hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a ... This gene encodes a member of glycosyl hydrolases family 31. This enzyme hydrolyses terminal, non-reducing 1,4-linked alpha-D- ... a new paralog in the glycosyl hydrolase gene family 31". Proceedings of the National Academy of Sciences of the United States ...
These digestive enzymes include proteases that digest proteins into amino acids, as well as glycoside hydrolases that digest ...
Neuraminidase is a type of glycoside hydrolase enzyme which helps to move the virus particles through the infected cell and ...
Glycoside hydrolases are classified into EC 3.2.1 as enzymes catalyzing the hydrolysis of O- or S-glycosides. Glycoside ... Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) catalyze the hydrolysis of glycosidic bonds in complex ... Mutant glycoside hydrolases termed glycosynthases have been developed that can achieve the synthesis of glycosides in high ... "Glycoside hydrolase" - news · newspapers · books · scholar · JSTOR (December 2010) (Learn how and when to remove this template ...
Glycoside hydrolase superfamily (IPR017853). Short name: Glycoside_hydrolase_SF Overlapping entries *Glycoside hydrolase family ... Glycoside hydrolase, family 2, active site (IPR023232). *Uncharacterised protein family, glycosyl hydrolase catalytic domain ( ... Glycoside hydrolase, family 3, N-terminal domain superfamily (IPR036962). *Alpha-glucuronidase, C-terminal domain superfamily ( ... The mechanisms by which family 10 glycoside hydrolases bind decorated substrates.. J. Biol. Chem. 279 9597-605 2004 ...
Treatment of A. fumigatus with Glycoside Hydrolases.. To visualize the effects of hydrolases on cell wall-associated GAG, ... Microbial glycoside hydrolases as antibiofilm agents with cross-kingdom activity. Brendan D. Snarr, Perrin Baker, Natalie C. ... Microbial glycoside hydrolases as antibiofilm agents with cross-kingdom activity Message Subject (Your Name) has sent you a ... Effects of Glycoside Hydrolases on A. fumigatus-Induced Epithelial Cell Damage.. A549 pulmonary epithelial cell damage by A. ...
Glycoside hydrolase, family 45 (IPR000334). Short name: Glyco_hydro_45 Domain relationships *RlpA-like protein, double-psi beta ... Glycoside hydrolase family 45 GH45 comprises enzymes with only one known activity; endoglucanase (EC:3.2.1.4). ... O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more ... One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [PMID: 8352747]. The best ...
In molecular biology, glycoside hydrolase family 70 is a family of glycoside hydrolases. Glycoside hydrolases EC 3.2.1. are a ... "Glycoside Hydrolase Family 70". CAZypedia.org. Retrieved 2018-03-06. CAZypedia Consortium (December 2018). "Ten years of ... A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different ... Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853-9. doi: ...
Glycoside hydrolases and glycosyltransferases: families and functional modules.. Bourne Y1, Henrissat B. ... The past year has witnessed the expected increase in the number of solved structures of glycoside hydrolases and ... These structures show that, while glycoside hydrolases display an extraordinary variety of folds, glycosyltransferases and ...
Glycoside Hydrolases. Accession Number. DBCAT000166. Description. Not Available. Drugs. Drug. Drug Description. ...
Glycoside Hydrolase Genes Are Required for Virulence of Agrobacterium tumefaciens on Bryophyllum daigremontiana and Tomato A. ... Manipulation of glycoside hydrolases may increase transformation and widen the host range of the bacterium.... ...
Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases. Jennifer ... In contrast, metagenomic analysis of a bovine rumen expression library (17) identified 22 glycoside hydrolase (GH) clones of ... A comparison of the glycoside hydrolase and cellulosome functional genes revealed that in the rumen microbiome, initial ... Furthermore, when compared with the termite hindgut microbiome, there are fundamental differences in the glycoside hydrolase ...
Strategies to reduce end-product inhibition in family 48 glycoside hydrolases Journal Article Chen, Mo ; Bu, Lintao ; Alahuhta ... 09 BIOMASS FUELS; Caldicellulosiruptor; lignocellulose; glycoside hydrolase; cellulase. OSTI Identifier:. 1475518. Alternate ... Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species Journal ... We also suggested a series of single mutants of the four family 48 glycoside hydrolases with theoretically reduced level of ...
Compounds that inhibit or block the activity of GLYCOSIDE HYDROLASES such as ALPHA-AMYLASES and APHA-GLUCOSIDASES.. *Drugs ...
As part of a program to discover improved glycoside hydrolase family 12 (GH 12) endoglucanases, we have studied the biochemical ... Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability Protein Sci. 2003 Apr; ... As part of a program to discover improved glycoside hydrolase family 12 (GH 12) endoglucanases, we have studied the biochemical ...
... in a bioreactor to select for a switchgrass-adapted community and to facilitate targeted discovery of glycoside hydrolases. ... of 454-titanium pyrosequence data from the SAC community and conservatively identified 800 genes encoding glycoside hydrolase ... Glycoside hydrolases Is the Subject Area "Glycoside hydrolases" applicable to this article? Yes. No. ...
Navbox , name = Sugar hydrolases , title = [[Hydrolase]]: sugar hydrolases ([[EC number,EC]] 3.2) , titlestyle = background:# ... Glycoside hydrolase]]s , list1 = [[Amylase]] ([[Alpha-Amylase]]) - [[Chitinase]] - [[Lysozyme]] - [[Neuraminidase]] - [[ ... View source for Template:Glycoside hydrolases. ← Template:Glycoside hydrolases. Jump to: navigation, search ...
Glycoside hydrolase family 61Imported. Automatic assertion inferred from database entriesi ... tr,G0R6T8,G0R6T8_HYPJQ Glycoside hydrolase family 61 OS=Hypocrea jecorina (strain QM6a) OX=431241 GN=cel61a PE=1 SV=1 ... HydrolaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/ ...
Metagenomic mining of glycoside hydrolases from the hindgut bacterial symbionts of a termite (Trinervitermes trinervoides) and ...
... Eriksson, Torny LU (2003) *Mark ... The majority of fungal glycoside hydrolases are glycoproteins. In Paper VII the N- glycosylation of T. reesei Cel7B was ... In this thesis, fungal glycoside hydrolases, cellulases and hemicellulases were studied with the aim of increasing our ... In this thesis, fungal glycoside hydrolases, cellulases and hemicellulases were studied with the aim of increasing our ...
Glycoside hydrolase family 1Imported. ,p>Information which has been imported from another database using automatic procedures ... tr,C6CAA0,C6CAA0_DICP7 Glycoside hydrolase family 1 OS=Dickeya paradisiaca (strain Ech703) GN=Dd703_0764 PE=3 SV=1 ... HydrolaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/ ... Belongs to the glycosyl hydrolase 1 family.UniRule annotation. ,p>Information which has been generated by the UniProtKB ...
CATALYSIS AND SPECIFICITY IN ENZYMATIC GLYCOSIDE HYDROLASES: A 2,5B CONFORMATION FOR THE GLYCOSYL-ENZYME INTERMIDIATE REVEALED ... CATALYSIS AND SPECIFICITY IN ENZYMATIC GLYCOSIDE HYDROLASES: A 2,5B CONFORMATION FOR THE GLYCOSYL-ENZYME INTERMIDIATE REVEALED ...
The glycoside hydrolase (GH) family 61 is a long-recognized, but still recondite, class of proteins, with little known about ... The glycoside hydrolase (GH) family 61 is a long-recognized, but still recondite, class of proteins, with little known about ... The structure of a glycoside hydrolase family 61 member, Cel61B from the Hypocrea jecorina.. *DOI: 10.2210/pdb2VTC/pdb ... The First Structure of a Glycoside Hydrolase Family 61 Member, Cel61B from the Hypocrea Jecorina, at 1.6 A Resolution.. ...
The SCOP classification for the Glycoside hydrolase/deacetylase superfamily including the families contained in it. Additional ... Glycoside hydrolase/deacetylase superfamily. SCOP classification Root: SCOP hierarchy in SUPERFAMILY [. 0] (11) ... This entry represents a structural motif found in glycoside hydrolase families 38 (. , e.g. alpha-mannosidase) [. 12960159] and ... There are 23 hidden Markov models representing the Glycoside hydrolase/deacetylase superfamily. Information on how the models ...
... Adlercreutz, Patrick LU (2017) In Applied ... Glycoside hydrolase, Lipase, Transesterification, Transglycosylation. in Applied Microbiology and Biotechnology. volume. 101. ... Lipases and glycoside hydrolases have large similarities concerning reaction mechanisms. Acyl-enzyme intermediates are formed ... Lipases and glycoside hydrolases have large similarities concerning reaction mechanisms. Acyl-enzyme intermediates are formed ...
C. perfringens produces a battery of secreted carbohydrate-active enzymes called glycoside hydrolases whose activities suggest ... perfringens glycoside hydrolases. Various X-ray and NMR structures of CpGH31 and CpGH84A CBM32s, both in apo form and in ...
A chitinase gene belonging to the glycoside hydrolase family 19 from Vibrio proteolyticus (chi19) was cloned. The recombinant ... A Reducing-End-Acting Chitinase From Vibrio Proteolyticus Belonging to Glycoside Hydrolase Family 19 Appl Microbiol Biotechnol ... A chitinase gene belonging to the glycoside hydrolase family 19 from Vibrio proteolyticus (chi19) was cloned. The recombinant ...
Keywords: glycoside hydrolase family 3; β-glucosidase; biodegradation; crystal structure; Neurospora crassa; NcCel3A. ... We have determined the structure of a glycoside hydrolase family 3 β-glucosidase, Cel3A from N. crassa, at 2.2 Å resolution and ... The glycoside hydrolase family 3 (GH3) β-glucosidases are a structurally diverse family of enzymes. Cel3A from Neurospora ... Glycoside hydrolase family 3 (GH3) is one of the larger families in the CAZy classification and currently contains over 13 600 ...
Glycoside hydrolases (GHs) are key enzymes in the depolymerization of plant-derived cellulose, a process central to the global ... Title: Comparative Community Proteomics Demonstrates the Unexpected Importance of Actinobacterial Glycoside Hydrolase Family 12 ... Comparative Community Proteomics Demonstrates the Unexpected Importance of Actinobacterial Glycoside Hydrolase Family 12 ...
3 The glycoside hydrolases PelAh and PslGh are noncytotoxic.. (A) IMR-90 cellomics assay to measure the length-to-width ratio ( ... 4 Glycoside hydrolases potentiate antibiotics and increase human neutrophil killing.. (A) CFUs for PAO1 ΔwspF Δpsl PBADpel ( ... 2 The glycoside hydrolases PelAh and PslGh catalyze the inhibition and disruption of P. aeruginosa biofilms.. (A) Crystal ... 1 The glycoside hydrolases PslGh and PelAh hydrolyze the exopolysaccharides Pel and Psl in a biofilm.. Representative confocal ...
Glycoside hydrolases (GHs) are enzymes that are able to rearrange the plant cell wall polysaccharides, being developmental- and ... Glycoside hydrolases (GHs) are enzymes that are able to rearrange the plant cell wall polysaccharides, being developmental- and ... Glycoside Hydrolases in Plant Cell Wall Proteomes: Predicting Functions That Could Be Relevant for Improving Biomass ... Glycoside Hydrolases in Plant Cell Wall Proteomes: Predicting Functions That Could Be Relevant for Improving Biomass ...
In molecular biology, glycoside hydrolase family 31 is a family of glycoside hydrolases. Glycoside hydrolases EC 3.2.1. are a ... A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of ,100 different ... Glycoside Hydrolase Family 31, 978-620-0-10221-8, Please note that the content of this book primarily consists of articles ... In molecular biology, glycoside hydrolase family 31 is a family of glycoside hydrolases. Glycoside hydrolases EC 3.2.1. are a ...
  • In higher organisms glycoside hydrolases are found within the endoplasmic reticulum and Golgi apparatus where they are involved in processing of N-linked glycoproteins , and in the lysosome as enzymes involved in the degradation of carbohydrate structures. (wikipedia.org)
  • Glycoside hydrolases are classified into EC 3.2.1 as enzymes catalyzing the hydrolysis of O- or S-glycosides. (wikipedia.org)
  • Glycoside hydrolases can also be classified according to the stereochemical outcome of the hydrolysis reaction: thus they can be classified as either retaining or inverting enzymes. (wikipedia.org)
  • A comparison of the glycoside hydrolase and cellulosome functional genes revealed that in the rumen microbiome, initial colonization of fiber appears to be by organisms possessing enzymes that attack the easily available side chains of complex plant polysaccharides and not the more recalcitrant main chains, especially cellulose. (pnas.org)
  • C. perfringens produces a battery of secreted carbohydrate-active enzymes called glycoside hydrolases whose activities suggest that their substrates are complex eukaryotic glycans, such as those found in the mucosal layer of the gut. (queensu.ca)
  • The glycoside hydrolase family 3 (GH3) β -glucosidases are a structurally diverse family of enzymes. (iucr.org)
  • Glycoside hydrolases (GHs) are key enzymes in the depolymerization of plant-derived cellulose, a process central to the global carbon cycle and the conversion of plant biomass to fuels and chemicals. (osti.gov)
  • Glycoside hydrolases (GHs) are enzymes that are able to rearrange the plant cell wall polysaccharides, being developmental- and stress-regulated. (intechopen.com)
  • The goal of this project was to circumvent the need to cultivate cellulolytic thermophiles by applying a metatranscriptomic approach to discover new thermostable cellulases commonly referred to as glycoside hydrolases (GH-enzymes that hydrolyze the glycosidic linkages between sugar molecules). (hawaii.edu)
  • Background: The large Glycoside Hydrolase family 5 (GH5) groups together a wide range of enzymes acting on beta-linked oligo- and polysaccharides, and glycoconjugates from a large spectrum of organisms. (diva-portal.org)
  • One of the most versatile and the largest of these GH families, containing enzymes with numerous biomass-deconstructing activities, is glycoside hydrolase family 5 (GH5). (diva-portal.org)
  • To investigate whether there are fundamental differences in the molecular architecture and biochemical properties of GH5 and GH26 mannanases, four genes encoding these enzymes were isolated from Cellvibrio japonicus and the encoded glycoside hydrolases were characterized. (portlandpress.com)
  • Structural and functional analyses of glycoside hydrolase 138 enzymes targeting chain A galacturonic acid in the complex pectin rhamnogalacturonan II. (jic.ac.uk)
  • As classified by the Carbohydrate-Active Enzymes (CAZy) database, enzymes in glycoside hydrolase (GH) family 10 (GH10) are all monospecific or bifunctional xylanases (except a tomatinase), and no endo-β-1,4-glucanase has been reported in the family. (asm.org)
  • Glycoside hydrolases (GH) are enzymes that hydrolyze glycosidic bonds between carbohydrates. (iastate.edu)
  • This furnished highly selective diamagnetic CEST agents that detected and quantified enzyme activities of glycoside hydrolase enzymes. (confex.com)
  • Glycoside hydrolases (GHs) are enzymes that catalyze the hydrolysis of the glycosidic bond between two carbohydrate residues or a carbohydrate unit linked to a non-carbohydrate aglycon unit. (iastate.edu)
  • QM/MM metadynamics simulations of two glycoside hydrolases, GH8 endoglucanase and GH43 β-xylosidase, give insight into the enzymatic deconstruction of the plant cell wall, and the detailed reaction mechanism of both enzymes. (iastate.edu)
  • Using cluster analysis, 319 human glycoside hydrolases were classified into four clusters based on their similarity to enzymes conserved in chordates or metazoans (Class 1), metazoans (Class 2), metazoans and plants (Class 3), and eukaryotes (Class 4). (preprints.org)
  • Photosynthate within plants is transported from source to sink tissues as sucrose, which is hydrolyzed by plant glycosyl hydrolase family 32 enzymes (GH32) into its constituent monosaccharides to meet plant cellular demands. (biomedcentral.com)
  • Invertase enzymes belong to the glycoside hydrolase family 32 (GH32) group of carbohydrate active enzymes, where family membership is designated based upon amino acid sequence conservation [ 5 ]. (biomedcentral.com)
  • As recently published in the Proceedings of the National Academy of Sciences (PNAS) journal, a team led by GlycoNet investigators Drs. Donald Sheppard and Lynne Howell have identified glycosyl hydrolases, a class of enzymes that modulate the removal of sugar residues, as prime targets in combating biofilm formation. (canadianglycomics.ca)
  • Excitingly, bacterial hydrolase enzymes were equally active against fungal biofilms, suggesting that some of these enzymes may be useful therapeutic agents in cases of co-colonization, such as in cystic fibrosis patients, as well as for a wide range of microbes. (canadianglycomics.ca)
  • To properly colonize their human hosts both bacteria have developed an extensive repertoire of glycoside hydrolases (GHs) which are enzymes responsible for the breakdown of carbohydrates. (uvic.ca)
  • Glycoside hydrolases (GHs) are enzymes hydrolyzing the glycosidic bond between carbohydrates or between a carbohydrate and a non-carbohydrate moiety [ 1 ]. (biomedcentral.com)
  • Glycoside hydrolases (GHs) are very important enzymes that can catalyze the breakdown of the glycosidic bonds between carbohydrates and non-carbohydrates to synthesize GOS prebiotic sugars and hydrolyze lactose in the dairy industry. (sciepub.com)
  • Among of them, enzymes involved in carbohydrate metabolismare designated carbohydrate-active enzymes (CAZy), and include glycosyltransferases (GTs), glycoside hydrolases (GHs), polysaccharide lyases (PLs), and carbohydrate esterases (CEs). (springer.com)
  • They belong to glycoside hydrolase families GH13 and GH57 and several of these enzymes are industrially important. (dtu.dk)
  • Glycoside hydrolases, the enzymes in charge of hydrolysis from the glycosidic relationship in di-, oligo- and polysaccharides, and glycoconjugates, are ubiquitous in Character and fundamental to existence. (lifescienceexec.com)
  • In this thesis, the structure-function relationship of three different microbial endo -xyloglucanases from glycoside hydrolase families 5, 12 and 44 are probed and reveal details of the natural diversity found in xyloglucanases. (diva-portal.org)
  • The observation that XXXG- β -Res is also hydrolyzed by diverse microbial XEHs indicates that this substrate, and resorufin glycosides in general, may find broad applicability for the analysis of wall restructuring by polysaccharide hydrolases during morphogenesis and plant-microbe interactions. (plantphysiol.org)
  • Glycoside hydrolases (GHs) comprise a significant proportion of the genes encoded by microbial genomes within the human distal gut microbiome and contribute to the depolymerization of recalcitrant dietary polysaccharides. (grantome.com)
  • Although a number of species of cultured bacteria are known to degrade crystalline cellulose, little is known of the polysaccharide hydrolases expressed by cellulose-degrading microbial communities, particularly in the marine environment. (mdpi.com)
  • Triticum aestivum xylanase inhibitor (TAXI)-type inhibitors are active against microbial xylanases from glycoside hydrolase family 11, but the inhibition strength and the specificity towards different xylanases differ between TAXI isoforms. (wiley.com)
  • Glycoside hydrolases (also called glycosidases or glycosyl hydrolases ) catalyze the hydrolysis of glycosidic bonds in complex sugars . (wikipedia.org)
  • In this thesis, fungal glycoside hydrolases, cellulases and hemicellulases were studied with the aim of increasing our knowledge of the mechanisms involved in the enzymatic hydrolysis of cellulose and lignocellulose. (lu.se)
  • Esen, Asim 2004-12-30 00:00:00 In plants, Glycoside Hydrolase (GH) Family 1 β-glycosidases are believed to play important roles in many diverse processes including chemical defense against herbivory, lignification, hydrolysis of cell wall-derived oligosaccharides during germination, and control of active phytohormone levels. (deepdyve.com)
  • Glycoside hydrolases (also called glycosidases ) catalyze the hydrolysis of the glycosidic linkage to generate two smaller sugars. (chemeurope.com)
  • Glycoside hydrolases (GH) catalyze the hydrolysis of glycosidic bonds in cell wall polymers and may have major results on cell wall structure architecture. (isvhld2012.org)
  • The classical systems for glycoside hydrolysis had been first suggested by Koshland in 195330 and, today more than 50 years afterwards, have got stood the check of period and a huge quantity of biochemical analysis and remain generally unchanged (for testimonials discover Ref. 31C33). (lifescienceexec.com)
  • In the next (deglycosylation) stage the acidity/bottom residue deprotonates 57-22-7 manufacture a drinking water molecule which gives a nucleophilic types to attack on the anomeric carbon and displace the glycoside.34,35 Open up in another window Fig. 1 Glycosidase systems for hydrolysis. (lifescienceexec.com)
  • One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [ PMID: 8352747 ]. (ebi.ac.uk)
  • We showed the functions of PhLP1, GNB1 and GNG1 in the same pathway, with one major output being the regulation of transcription of glycoside hydrolase genes including cellulase genes in T. reesei . (biomedcentral.com)
  • The research reported here is the first designed to specifically address the bacterial communities that colonize and degrade cellulose in the marine environment and to evaluate the glycoside hydrolase (cellulase and chitinase) gene repertoire of that community, in the absence of the biases associated with PCR-based molecular techniques. (mdpi.com)
  • Glycoside hydrolase (GH) family 48 is an understudied and increasingly important exoglucanase family found in the majority of bacterial cellulase systems. (biomedcentral.com)
  • Importantly, this new in situ XEH assay provides an essential complement to the in situ xyloglucan endotransglycosylase assay, thus allowing delineation of the disparate activities encoded by xyloglucan endotransglycosylase / hydrolase genes directly in plant tissues. (plantphysiol.org)
  • Furthermore, the identification of 211 gene sequences matched to a custom-made database comprising the members of nine glycoside hydrolase families revealed an extensive repertoire of functional genes predicted to be involved in cellulose utilization. (mdpi.com)
  • Results and dialogue Classification of 437 glycoside hydrolase genes in grain into 34 family members When accessed because of this research, the CAZy data source (http://www.cazy.org/Glycoside-Hydrolases.html) contained 430 genes annotated while coding for glycoside hydrolases in grain. (isvhld2012.org)
  • Population dynamics of library clones revealed Bt genes conferring significant fitness advantages in E. coli over time, including carbohydrate utilization genes, with a Bt galactokinase central to early colonization, and subsequent dominance by a Bt glycoside hydrolase enabling sucrose metabolism coupled with co-evolution of the plasmid library and E. coli genome driving increased galactose utilization. (nih.gov)
  • Glycoside hydrolase family 3 (GH3) is one of the larger families in the CAZy classification and currently contains over 13 600 annotated protein sequences. (iucr.org)
  • The heterotrimeric G-protein pathway is crucial for the interconnection of nutrient signaling and light response of T. reesei , with the class I phosducin-like protein PhLP1, GNB1 and GNG1 acting as important nodes, which influence light responsiveness, glycoside hydrolase gene transcription and sexual development. (biomedcentral.com)
  • We identified a glycoside hydrolase family 12 (GH12) protein, XEG1, produced by the soybean pathogen Phytophthora sojae that exhibits xyloglucanase and β-glucanase activity. (oregonstate.edu)
  • Crystallographic studies of ReCel3A revealed a highly N-glycosylated three-domain dimeric protein, as has been observed previously for glycoside hydrolase family 3 beta-glucosidases. (diva-portal.org)
  • 1 proteins predicted to have the Glycoside hydrolase, family 37 protein domain from the InterPro Predicted Protein Domain Annotations dataset. (mssm.edu)
  • Protein sequence network for the chitinase domains from the Glycoside Hydrolase 19 Engineering Database. (uni-stuttgart.de)
  • Protein sequence network for the endolysin domains from the Glycoside Hydrolase 19 Engineering Database. (uni-stuttgart.de)
  • Protein sequence network for representative GH19 domains (corresponding to Pfam's GH19 profile HMM: PF00182) from the Glycoside Hydrolase 19 Engineering Database. (uni-stuttgart.de)
  • GraphML files for undirected weighted graphs with nodes that represent protein sequences of glycoside hydrolase 19 homologues. (uni-stuttgart.de)
  • Robb, CS, Mystkowska, AA and Hehemann, JH (2017) Crystal structure of a marine glycoside hydrolase family 99-related protein lacking catalytic machinery. (marum.de)
  • In this doctoral study, we focussed on three main subjects dealing withthe differences in substrate specificity between the different types ofGH32 family hydrolases. (kuleuven.be)
  • To determine the biological function of all family members, we intend to investigate the substrate specificity of each mature hydrolase after its heterologous expression in the Pichia pastoris expression system. (deepdyve.com)
  • A chitinase gene belonging to the glycoside hydrolase family 19 from Vibrio proteolyticus (chi19) was cloned. (nih.gov)
  • The ability to degrade polymeric α -glucans is confined to few strains encoding extracellular amylolytic activities of glycoside hydrolase (GH) family 13. (springer.com)
  • On the other hand, synthesis applications of glycoside hydrolases are much less developed. (lu.se)
  • Glycoside hydrolases and glycosyltransferases: families and functional modules. (nih.gov)
  • Consequently, the identified proteins belonged to the functional categories of carbohydrate transport & metabolism and hydrolase activity. (hoajonline.com)
  • Carbohydrate processing by bacterial pathogens: structural and functional analyses of glycoside hydrolases. (uvic.ca)
  • The glycoside hydrolases are involved in the biosynthesis and degradation of glycogen in the body. (wikipedia.org)
  • We found no direct correlation between the growth rate and global regulation of glycoside hydrolases, which suggests that regulation of growth does not occur only at the level of substrate degradation efficiency. (biomedcentral.com)
  • Lab results confirmed that glycosyl hydrolase treatment led to degradation and prevention of biofilms, reduced virulence, and increased response to antimicrobial therapeutics. (canadianglycomics.ca)
  • Here, we report the biochemical characterization of the endo-beta-1,4-(xylo)glucan hydrolase from Paenibacillus polymyxa with polymeric, oligomeric, and defined chromogenic aryl-oligosaccharide substrates. (diva-portal.org)
  • Molecular cloning and characterization of Bifidobacterium bifidum 1,2-alpha-L-fucosidase (AfcA), a novel inverting glycosidase (glycoside hydrolase family 95). (semanticscholar.org)
  • Two forms of Ruminococcus flavefaciens FD-1 endoglucanase B, a member of glycoside hydrolase family 44, one with only a catalytic domain and the other with a catalytic domain and a carbohydrate binding domain (CBM), were produced. (naver.com)
  • The team cloned and expressed glycosyl hydrolase domains from both P. aeruginosa and A. fumigatus in order to test their ability to disrupt pre-formed biofilms and prevent biofilm formation. (canadianglycomics.ca)
  • Biotransformation pathways of ginsenosides Rb1, Rd, Rb2, Rb3, and Rc by recombinant BglPm and incorporated pathways for F2 production by two glycoside hydrolases. (figshare.com)
  • Discovery of a Bacterial Glycoside Hydrolase Family 3 (GH3) β-Glucosidase with Myrosinase Activity from a Citrobacter Strain Isolated from Soil. (semanticscholar.org)
  • Plant FEHs are closely related to cell wallinvertases at the molecular and structural level and are grouped in theGlycoside Hydrolase Family 32 (GH32). (kuleuven.be)
  • The mostly happening structural fold in glycoside hydrolases in grain may be the (/)8 barrel, quality of 17 GH family members owned by the GH-A, GH-D, GH-K or GH-H clans aswell by the GH14, GH29 and GH89 family members (http://www.cazy.org/Glycoside-Hydrolases.html). (isvhld2012.org)
  • Crystallographic analysis of the structures of TAXI-IA and TAXI-IIA in complex with glycoside hydrolase family 11 B. subtilis xylanase A now provides a substantial explanation for these observations and a detailed insight into the structural determinants for inhibition strength and specificity. (wiley.com)
  • Henrissat B, Bairoch A. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. (ebi.ac.uk)
  • Structures and mechanisms of glycosyl hydrolases. (ebi.ac.uk)
  • Lipases and glycoside hydrolases have large similarities concerning reaction mechanisms. (lu.se)
  • The multifunctional glycoside hydrolase CoGH1A has high capabilities in saccharification of lignocellulosic biomass, decomposition of lactose, and synthesis of galactooligosaccharides. (biomedcentral.com)
  • Les Glycoside hydrolases (ou glycosidases) catalysent l'hydrolyse de liaisons glycosidiques et libèrent au moins un composé osidique. (dbpedia.org)
  • Given that the glycoside hydrolases PelA h and PslG h encoded in the pel and psl biosynthetic operons, respectively, are utilized for in vivo exopolysaccharide processing, we reasoned that these would provide specificity to target P. aeruginosa biofilms. (sciencemag.org)
  • Unraveling the subtleties of ß-(1?3)-glucan phosphorylase specificity in the GH94, GH149 and GH161 glycoside hydrolase families. (jic.ac.uk)
  • 2VTC: The structure of a glycoside hydrolase family 61 member, Cel61B from the Hypocrea jecorina. (rcsb.org)
  • The structure of a glycoside hydrolase family 61 member, Cel61B from the Hypocrea jecorina. (rcsb.org)
  • The glycoside hydrolase (GH) family 61 is a long-recognized, but still recondite, class of proteins, with little known about the activity, mechanism or function of its more than 70 members. (rcsb.org)
  • In plants, xyloglucans are modified in muro by the xyloglucan transglycosylase/hydrolase ( XTH ) gene products. (diva-portal.org)
  • The gene product (154 kDa) is unrelated to Beta-agarases and instead belongs to a new family of glycoside hydrolases (GH96). (ifremer.fr)
  • The past year has witnessed the expected increase in the number of solved structures of glycoside hydrolases and glycosyltransferases, and their constitutive modules. (nih.gov)
  • These structures show that, while glycoside hydrolases display an extraordinary variety of folds, glycosyltransferases and carbohydrate-binding modules appear to belong to a much smaller number of folding families. (nih.gov)
  • Glycans are involved in various metabolic processes via the functions of glycosyltransferases and glycoside hydrolases. (preprints.org)
  • Based on our previous study of glycosyltransferases, we performed a genome-wide analysis of whole human glycoside hydrolases using the UniProt, BRENDA, CAZy, and KEGG databases. (preprints.org)
  • In contrast, other potential virulence factors, such as Glycoside Hydrolases family 20 (hexosaminidase) and 37 (trehalase) proteins (GH20 and GH37), contained identifiable signal peptides. (altmetric.com)
  • The iodothyronine selenodeiodinases are thioredoxin-fold family proteins containing a glycoside hydrolase clan GH-A-like structure. (rush.edu)
  • The glycoside hydrolase (GH) proteins are found in a wide range of organisms viz. (hoajonline.com)
  • Glycoside hydrolases (GHs) have attracted considerable attention as targets for therapeutic agents, and thus mechanism-based inhibitors are of great interest. (st-andrews.ac.uk)
  • We have employed a multi-disciplinary "dissect-and-build" approach to characterize the molecular determinants of carbohydrate binding by CBM32s, and global glycan recognition by full-length C. perfringens glycoside hydrolases. (queensu.ca)
  • In molecular biology, glycoside hydrolase family 70 is a family of glycoside hydrolases. (wikipedia.org)
  • Representative confocal images of Psl biofilms grown statically for 24 hours ( top ) and Pel biofilms cultivated for 48 hours ( bottom ) under flow conditions and treated with wild-type hydrolases or hydrolases that have point mutations to catalytic residues. (sciencemag.org)
  • The first crystal structure of a family 129 glycoside hydrolase from a probiotic bacterium reveals critical residues and metal cofactors. (semanticscholar.org)
  • Kinetic analysis on defined oligosaccharides and on aryl-glycosides suggests that both the -4 and +1 subsites show discrimination against xylose-appended glucosides. (diva-portal.org)
  • Crystallographic structures of both the apo enzyme and the trapped covalent 5-fluoro-β-xylosyl-enzyme intermediate, together with docking studies with the XXXG heptasaccharide, revealed, for the first time in GH31 (glycoside hydrolase family 31), the importance of a PA14 domain insert in the recognition of longer oligosaccharides by extension of the active-site pocket. (portlandpress.com)
  • In addition, some GHs have the capability of transglycosylation and are used for synthesis of oligosaccharides and glycosides, such as galactooligosaccharides (GalOS) (synthesized by β-galactosidase) [ 3 ] and octyl glucoside (synthesized by β-glucosidase) [ 4 ]. (biomedcentral.com)
  • Glycoside hydrolases are found in the intestinal tract and in saliva where they degrade complex carbohydrates such as lactose , starch , sucrose and trehalose . (wikipedia.org)
  • Structure-function analysis of Glycoside Hydrolase Family 32 (GH32) plant invertases and fructan exohydrolases Fructans are sucrose based polymers of fructose occuring in about 15% of the flowering plants. (kuleuven.be)
  • The majority of fungal glycoside hydrolases are glycoproteins. (lu.se)
  • Fourteen clans called from GH-A to GH-N, have already been described for glycoside hydrolases in the CAZy data source (http://www.cazy.org/Glycoside-Hydrolases.html). (isvhld2012.org)
  • One of the important occurrences of glycoside hydrolases in bacteria is the enzyme beta-galactosidase (LacZ), which is involved in regulation of expression of the lac operon in E. coli . (wikipedia.org)
  • Previously, we characterized ß-(1?3)-glucan GPs from bacteria and E. gracilis, leading to their classification in glycoside hydrolase family GH149. (jic.ac.uk)
  • In addition, the results of this study indicate that the extremely thermophilic bacteria are potential resources for screening highly efficient glycoside hydrolases for the production of biofuels and biochemicals. (biomedcentral.com)
  • In addition, as each organism produces similar glycan structures to form biofilms, the team hypothesized that the glycosyl hydrolases would exhibit cross-species activity. (canadianglycomics.ca)
  • Fig. 1 The glycoside hydrolases PslG h and PelA h hydrolyze the exopolysaccharides Pel and Psl in a biofilm. (sciencemag.org)
  • Exploring efficient thermophilic glycoside hydrolases will accelerate the industrialization of biofuels and biochemicals. (biomedcentral.com)
  • A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of more than 100 different families. (wikipedia.org)
  • Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. (ebi.ac.uk)
  • One interesting class of CAZymes is the group of glycoside hydrolases (GHs) containing more than 138000 modules divided into 131 families as of February 2013. (diva-portal.org)
  • β-1,4-Mannanases (mannanases), which hydrolyse mannans and glucomannans, are located in glycoside hydrolase families (GHs) 5 and 26. (portlandpress.com)
  • Although both the α-amylase super-family, i.e. the glycoside hydrolase (GH) clan GH-H (the GH families 13, 70 and 77), and family GH31 share some characteristics, their different catalytic machinery prevents classification of GH31 in clan GH-H. A significant but remote evolutionary relatedness is, however, proposed for clan GH-H with GH31. (dtu.dk)
  • The open Caldicellulosiruptor pangenome encoded 106 glycoside hydrolases (GHs) from 43 GH families [ 7 ]. (biomedcentral.com)
  • As part of a program to discover improved glycoside hydrolase family 12 (GH 12) endoglucanases, we have studied the biochemical diversity of several GH 12 homologs. (nih.gov)
  • The transglycosylases, hydrolases which naturally catalyze mainly transfer reactions, are of special interest and might be useful guides for engineering of other hydrolases. (lu.se)
  • Fig. 2 The glycoside hydrolases PelA h and PslG h catalyze the inhibition and disruption of P. aeruginosa biofilms. (sciencemag.org)
  • Glycoside phosphorylases (GPs) catalyze the phosphorolysis of glycans into the corresponding sugar 1-phosphates and shortened glycan chains. (jic.ac.uk)