Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Glycoproteins found on the membrane or surface of cells.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
Established cell cultures that have the potential to propagate indefinitely.
Proteins found in any species of virus.
Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)
The characteristic 3-dimensional shape of a carbohydrate.
The sum of the weight of all the atoms in a molecule.
A group of related enzymes responsible for the endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose-content glycopeptides and GLYCOPROTEINS.
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.
Proteins, usually glycoproteins, found in the viral envelopes of a variety of viruses. They promote cell membrane fusion and thereby may function in the uptake of the virus by cells.
An N-acetylglycosamine containing antiviral antibiotic obtained from Streptomyces lysosuperificus. It is also active against some bacteria and fungi, because it inhibits the glucosylation of proteins. Tunicamycin is used as tool in the study of microbial biosynthetic mechanisms.
Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
An amidohydrolase that removes intact asparagine-linked oligosaccharide chains from glycoproteins. It requires the presence of more than two amino-acid residues in the substrate for activity. This enzyme was previously listed as EC 3.2.2.18.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The N-acetyl derivative of glucosamine.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An indolizidine alkaloid from the plant Swainsona canescens that is a potent alpha-mannosidase inhibitor. Swainsonine also exhibits antimetastatic, antiproliferative, and immunomodulatory activity.
Ubiquitously expressed integral membrane glycoproteins found in the LYSOSOME.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Proteins which are found in eggs (OVA) from any species.
Antibodies produced by a single clone of cells.
Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)
The N-acetyl derivative of galactosamine.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
Sites on an antigen that interact with specific antibodies.
The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.
Glycoprotein moieties on the surfaces of cell membranes that bind concanavalin A selectively; the number and location of the sites depends on the type and condition of the cell.
Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.
Retroviral proteins, often glycosylated, coded by the envelope (env) gene. They are usually synthesized as protein precursors (POLYPROTEINS) and later cleaved into the final viral envelope glycoproteins by a viral protease.
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
A group of enzymes with the general formula CMP-N-acetylneuraminate:acceptor N-acetylneuraminyl transferase. They catalyze the transfer of N-acetylneuraminic acid from CMP-N-acetylneuraminic acid to an acceptor, which is usually the terminal sugar residue of an oligosaccharide, a glycoprotein, or a glycolipid. EC 2.4.99.-.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
SUGARS containing an amino group. GLYCOSYLATION of other compounds with these amino sugars results in AMINOGLYCOSIDES.
Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.
The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by translocation of the whole virus across the cell membrane.
A lectin found in ENDOPLASMIC RETICULUM membranes that binds to specific N-linked OLIGOSACCHARIDES found on newly synthesized proteins. It may play role in PROTEIN FOLDING or retention and degradation of misfolded proteins in the endoplasmic reticulum.
Specific hemagglutinin subtypes encoded by VIRUSES.
A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.
The adherence and merging of cell membranes, intracellular membranes, or artificial membranes to each other or to viruses, parasites, or interstitial particles through a variety of chemical and physical processes.
A species of VARICELLOVIRUS producing a respiratory infection (PSEUDORABIES) in swine, its natural host. It also produces an usually fatal ENCEPHALOMYELITIS in cattle, sheep, dogs, cats, foxes, and mink.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
External envelope protein of the human immunodeficiency virus which is encoded by the HIV env gene. It has a molecular weight of 120 kDa and contains numerous glycosylation sites. Gp120 binds to cells expressing CD4 cell-surface antigens, most notably T4-lymphocytes and monocytes/macrophages. Gp120 has been shown to interfere with the normal function of CD4 and is at least partly responsible for the cytopathic effect of HIV.
Enzymes that catalyze the transfer of galactose from a nucleoside diphosphate galactose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
Glycoproteins attached to the surface coat of the trypanosome. Many of these glycoproteins show amino acid sequence diversity expressed as antigenic variations. This continuous development of antigenically distinct variants in the course of infection ensures that some trypanosomes always survive the development of immune response to propagate the infection.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)
A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
Multinucleated masses produced by the fusion of many cells; often associated with viral infections. In AIDS, they are induced when the envelope glycoprotein of the HIV virus binds to the CD4 antigen of uninfected neighboring T4 cells. The resulting syncytium leads to cell death and thus may account for the cytopathic effect of the virus.
Glycoprotein from Sendai, para-influenza, Newcastle Disease, and other viruses that participates in binding the virus to cell-surface receptors. The HN protein possesses both hemagglutinin and neuraminidase activity.
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
A strong oxidizing agent.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.
A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A class of inorganic or organic compounds that contain the borohydride (BH4-) anion.
Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
The systematic study of the structure and function of the complete set of glycans (the glycome) produced in a single organism and identification of all the genes that encode glycoproteins.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
Substances elaborated by viruses that have antigenic activity.
A species of RESPIROVIRUS also called hemadsorption virus 2 (HA2), which causes laryngotracheitis in humans, especially children.
Cell surface receptors that bind to ACETYLGLUCOSAMINE.
The rate dynamics in chemical or physical systems.
A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A family of viruses, mainly arboviruses, consisting of a single strand of RNA. Virions are enveloped particles 90-120 nm diameter. The complete family contains over 300 members arranged in five genera: ORTHOBUNYAVIRUS; HANTAVIRUS; NAIROVIRUS; PHLEBOVIRUS; and TOSPOVIRUS.
Membrane glycoproteins from influenza viruses which are involved in hemagglutination, virus attachment, and envelope fusion. Fourteen distinct subtypes of HA glycoproteins and nine of NA glycoproteins have been identified from INFLUENZA A VIRUS; no subtypes have been identified for Influenza B or Influenza C viruses.
The type species of VESICULOVIRUS causing a disease symptomatically similar to FOOT-AND-MOUTH DISEASE in cattle, horses, and pigs. It may be transmitted to other species including humans, where it causes influenza-like symptoms.
A beta-N-Acetylhexosaminidase that catalyzes the hydrolysis of terminal, non-reducing 2-acetamido-2-deoxy-beta-glucose residues in chitobiose and higher analogs as well as in glycoproteins. Has been used widely in structural studies on bacterial cell walls and in the study of diseases such as MUCOLIPIDOSIS and various inflammatory disorders of muscle and connective tissue.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Proteins prepared by recombinant DNA technology.
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
Transmembrane envelope protein of the HUMAN IMMUNODEFICIENCY VIRUS which is encoded by the HIV env gene. It has a molecular weight of 41,000 and is glycosylated. The N-terminal part of gp41 is thought to be involved in CELL FUSION with the CD4 ANTIGENS of T4 LYMPHOCYTES, leading to syncytial formation. Gp41 is one of the most common HIV antigens detected by IMMUNOBLOTTING.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
An envelope protein of the human immunodeficiency virus that is encoded by the HIV env gene. It has a molecular weight of 160,000 kDa and contains numerous glycosylation sites. It serves as a precursor for both the HIV ENVELOPE PROTEIN GP120 and the HIV ENVELOPE PROTEIN GP41.
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
Enzymes that catalyze the exohydrolysis of 1,4-alpha-glucosidic linkages with release of alpha-glucose. Deficiency of alpha-1,4-glucosidase may cause GLYCOGEN STORAGE DISEASE TYPE II.
A proteolytic enzyme obtained from Streptomyces griseus.
A multifunctional protein that is found primarily within membrane-bound organelles. In the ENDOPLASMIC RETICULUM it binds to specific N-linked oligosaccharides found on newly-synthesized proteins and functions as a MOLECULAR CHAPERONE that may play a role in PROTEIN FOLDING or retention and degradation of misfolded proteins. In addition calreticulin is a major storage form for CALCIUM and functions as a calcium-signaling molecule that can regulate intracellular calcium HOMEOSTASIS.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
Lectin purified from peanuts (ARACHIS HYPOGAEA). It binds to poorly differentiated cells and terminally differentiated cells and is used in cell separation techniques.
Phosphoric acid esters of mannose.
Proteins encoded by the ENV GENE of the HUMAN IMMUNODEFICIENCY VIRUS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.
Enzymes that hydrolyze O-glucosyl-compounds. (Enzyme Nomenclature, 1992) EC 3.2.1.-.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
The photography of images produced on a fluorescent screen by X-rays.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
Enzymes catalyzing the transfer of fucose from a nucleoside diphosphate fucose to an acceptor molecule which is frequently another carbohydrate, a glycoprotein, or a glycolipid molecule. Elevated activity of some fucosyltransferases in human serum may serve as an indicator of malignancy. The class includes EC 2.4.1.65; EC 2.4.1.68; EC 2.4.1.69; EC 2.4.1.89.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
A genus of the family PARAMYXOVIRIDAE (subfamily PARAMYXOVIRINAE) where all the virions have both HEMAGGLUTININ and NEURAMINIDASE activities and encode a non-structural C protein. SENDAI VIRUS is the type species.
A species of HENIPAVIRUS, closely related to HENDRA VIRUS, which emerged in Peninsular Malaysia in 1998. It causes a severe febrile VIRAL ENCEPHALITIS in humans and also encephalitis and RESPIRATORY TRACT INFECTIONS in pigs. Fruit bats (PTEROPUS) are the natural host.
Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.
Inorganic salts of sulfuric acid.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Glycoprotein molecules on the surface of B- and T-lymphocytes, that react with molecules of antilymphocyte sera, lectins, and other agents which induce blast transformation of lymphocytes.
A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.
A species of ALPHAVIRUS isolated in central, eastern, and southern Africa.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Disorders caused by abnormalities in platelet count or function.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A species of RESPIROVIRUS frequently isolated from small children with pharyngitis, bronchitis, and pneumonia.
The major human blood type system which depends on the presence or absence of two antigens A and B. Type O occurs when neither A nor B is present and AB when both are present. A and B are genetic factors that determine the presence of enzymes for the synthesis of certain glycoproteins mainly in the red cell membrane.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A species of VARICELLOVIRUS that causes INFECTIOUS BOVINE RHINOTRACHEITIS and other associated syndromes in CATTLE.
A nucleoside diphosphate sugar which can be converted to the deoxy sugar GDPfucose, which provides fucose for lipopolysaccharides of bacterial cell walls. Also acts as mannose donor for glycolipid synthesis.
A subclass of lectins that are specific for CARBOHYDRATES that contain MANNOSE.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.
A genus of TOGAVIRIDAE, also known as Group A arboviruses, serologically related to each other but not to other Togaviridae. The viruses are transmitted by mosquitoes. The type species is the SINDBIS VIRUS.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The type species of MORBILLIVIRUS and the cause of the highly infectious human disease MEASLES, which affects mostly children.
Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A slightly alkaline secretion of the endocervical glands. The consistency and amount are dependent on the physiological hormone changes in the menstrual cycle. It contains the glycoprotein mucin, amino acids, sugar, enzymes, and electrolytes, with a water content up to 90%. The mucus is a useful protection against the ascent of bacteria and sperm into the uterus. (From Dictionary of Obstetrics and Gynecology, 1988)
A family of enveloped, linear, double-stranded DNA viruses infecting a wide variety of animals. Subfamilies, based on biological characteristics, include: ALPHAHERPESVIRINAE; BETAHERPESVIRINAE; and GAMMAHERPESVIRINAE.
A C-type lectin that is a cell surface receptor for ASIALOGLYCOPROTEINS. It is found primarily in the LIVER where it mediates the endocytosis of serum glycoproteins.
The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
Adherence of cells to surfaces or to other cells.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Sensitive assay using radiolabeled ANTIGENS to detect specific ANTIBODIES in SERUM. The antigens are allowed to react with the serum and then precipitated using a special reagent such as PROTEIN A sepharose beads. The bound radiolabeled immunoprecipitate is then commonly analyzed by gel electrophoresis.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
A family of RNA viruses, of the order MONONEGAVIRALES, containing filamentous virions. Although they resemble RHABDOVIRIDAE in possessing helical nucleocapsids, Filoviridae differ in the length and degree of branching in their virions. There are two genera: EBOLAVIRUS and MARBURGVIRUS.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Antibodies reactive with HIV ANTIGENS.
The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
A class of animal lectins that bind specifically to beta-galactoside in a calcium-independent manner. Members of this class are distiguished from other lectins by the presence of a conserved carbohydrate recognition domain. The majority of proteins in this class bind to sugar molecules in a sulfhydryl-dependent manner and are often referred to as S-type lectins, however this property is not required for membership in this class.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A genus in the family FILOVIRIDAE consisting of several distinct species of Ebolavirus, each containing separate strains. These viruses cause outbreaks of a contagious, hemorrhagic disease (HEMORRHAGIC FEVER, EBOLA) in humans, usually with high mortality.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
A species in the ORTHOBUNYAVIRUS genus of the family BUNYAVIRIDAE. A large number of serotypes or strains exist in many parts of the world. They are transmitted by mosquitoes and infect humans in some areas.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.

Neu differentiation factor stimulates phosphorylation and activation of the Sp1 transcription factor. (1/19351)

Neu differentiation factors (NDFs), or neuregulins, are epidermal growth factor-like growth factors which bind to two tyrosine kinase receptors, ErbB-3 and ErbB-4. The transcription of several genes is regulated by neuregulins, including genes encoding specific subunits of the acetylcholine receptor at the neuromuscular junction. Here, we have examined the promoter of the acetylcholine receptor epsilon subunit and delineated a minimal CA-rich sequence which mediates transcriptional activation by NDF (NDF-response element [NRE]). Using gel mobility shift analysis with an NRE oligonucleotide, we detected two complexes that are induced by treatment with neuregulin and other growth factors and identified Sp1, a constitutively expressed zinc finger phosphoprotein, as a component of one of these complexes. Phosphatase treatment, two-dimensional gel electrophoresis, and an in-gel kinase assay indicated that Sp1 is phosphorylated by a 60-kDa kinase in response to NDF-induced signals. Moreover, Sp1 seems to act downstream of all members of the ErbB family and thus may funnel the signaling of the ErbB network into the nucleus.  (+info)

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (2/19351)

Neurotrophins regulate survival, axonal growth, and target innervation of sensory and other neurons. Neurotrophin-3 (NT-3) is expressed specifically in cells adjacent to extending axons of dorsal root ganglia neurons, and its absence results in loss of most of these neurons before their axons reach their targets. However, axons are not required for NT-3 expression in limbs; instead, local signals from ectoderm induce NT-3 expression in adjacent mesenchyme. Wnt factors expressed in limb ectoderm induce NT-3 in the underlying mesenchyme. Thus, epithelial-mesenchymal interactions mediated by Wnt factors control NT-3 expression and may regulate axonal growth and guidance.  (+info)

A novel class of protein from wheat which inhibits xylanases. (3/19351)

We have purified a novel class of protein that can inhibit the activity of endo-beta-1,4-xylanases. The inhibitor from wheat (Triticum aestivum, var. Soisson) is a glycosylated, monomeric, basic protein with a pI of 8.7-8.9, a molecular mass of 29 kDa and a unique N-terminal sequence of AGGKTGQVTVFWGRN. We have shown that the protein can inhibit the activity of two family-11 endo-beta-1, 4-xylanases, a recombinant enzyme from Aspergillus niger and an enzyme from Trichoderma viride. The inhibitory activity is heat and protease sensitive. The kinetics of the inhibition have been characterized with the A. niger enzyme using soluble wheat arabinoxylan as a substrate. The Km for soluble arabinoxylan in the absence of inhibitor is 20+/-2 mg/ml with a kcat of 103+/-6 s-1. The kinetics of the inhibition of this reaction are competitive, with a Ki value of 0.35 microM, showing that the inhibitor binds at or close to the active site of free xylanase. This report describes the first isolation of a xylanase inhibitor from any organism.  (+info)

Structural characterization of the N-linked oligosaccharides in bile salt-stimulated lipase originated from human breast milk. (4/19351)

The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity and degree of fucosylation. Ion-exchange chromatography with pulsed amperometric detection, together with mass-spectral analysis of the esterified N- glycans, indicated the presence of monosialylated structures. The molecular mass profile of esterified N- glycans present in BSSL further permitted the more detailed studies through collision-induced dissociation (CID) and sequential exoglycosidase cleavages. The N- glycan structures were elucidated to be complex/dibranched, fucosylated/complex/dibranched, monosialylated/complex/dibranched, and monosialylated/fucosylated/dibranched entities.  (+info)

Gas-liquid chromatography of the heptafluorobutyrate derivatives of the O-methyl-glycosides on capillary columns: a method for the quantitative determination of the monosaccharide composition of glycoproteins and glycolipids. (5/19351)

We have developed a method involving the formation of hepta-fluorobutyrate derivatives of O-methyl-glycosides liberated from glycoproteins and glycolipids following methanolysis. The stable derivatives of the most common monosaccharides of these glycoconjugates (Ara, Rha, Xyl, Fuc, Gal, Man, Glc, GlcNAc, GalNAc, Neu5Ac, KDN) can be separated and quantitatively and reproducibly determined with a high degree of sensitivity level (down to 25 pmol) in the presence of lysine as an internal standard. The GlcNAc residue bound to Asn in N-glycans is quantitatively recovered as two peaks. The latter were easily distinguished from the other GlcNAc residues of N-glycans, thus allowing a considerable improvement of the data on structure of N-glycans obtained from a single carbohydrate analysis. The most common contaminants present in buffers commonly used for the isolation of soluble or membrane-bound glycoproteins (SDS, Triton X-100, DOC, TRIS, glycine, and polyacrylamide or salts, as well as monosaccharide constituents of proteoglycans or degradation products of nucleic acids) do not interfere with these determinations. A carbohydrate analysis of glycoproteins isolated from a SDS/PAGE gel or from PDVF membranes can be performed on microgram amounts without significant interferences. Since fatty acid methyl esters and sphingosine derivatives are separated from the monosaccharide peaks, the complete composition of gangliosides can be achieved in a single step starting from less than 1 microg of the initial compound purified by preparative Silicagel TLC. Using electron impact ionization mass spectrometry, reporter ions for the different classes of O-methyl-glycosides (pentoses, deoxy-hexoses, hexoses, hexosamines, uronic acids, sialic acid, and KDN) allow the identification of these compounds in very complex mixtures. The mass of each compound can be determined in the chemical ionization mode and detection of positive or negative ions. This method presents a considerable improvement compared to those using TMS derivatives. Indeed the heptafluorobutyrate derivatives are stable, and acylation of amino groups is complete. Moreover, there is no interference with contaminants and the separation between fatty acid methyl-esters and O-methyl glycosides is achieved.  (+info)

Antiphospholipid, anti-beta 2-glycoprotein-I and anti-oxidized-low-density-lipoprotein antibodies in antiphospholipid syndrome. (6/19351)

Antiphospholipid antibodies (aPL), anti-beta 2-glycoprotein I (anti-beta 2-GPI) and anti-oxidized-low-density lipoprotein (LDL) antibodies are all implicated in the pathogenesis of antiphospholipid syndrome. To investigate whether different autoantibodies or combinations thereof produced distinct effects related to their antigenic specificities, we examined the frequencies of antiphospholipid syndrome (APS)-related features in the presence of different antibodies [aPL, beta 2-GPI, anti-oxidized low density lipoprotein (LDL)] in 125 patients with APS. Median follow-up was 72 months: 58 patients were diagnosed as primary APS and 67 as APS plus systemic lupus erythematosus (SLE). Anticardiolipin antibodies (aCL), anti-beta 2-GPI and anti-oxidized LDL antibodies were determined by ELISA; lupus anticoagulant (LA) by standard coagulometric methods. Univariate analysis showed that patients positive for anti-beta 2-GPI had a higher risk of recurrent thrombotic events (OR = 3.64, 95% CI, p = 0.01) and pregnancy loss (OR = 2.99, 95% CI, p = 0.004). Patients positive for anti-oxidized LDL antibodies had a 2.24-fold increase in the risk of arterial thrombosis (2.24, 95% CI, p = 0.03) and lower risk of thrombocytopenia (OR = 0.41 95% CI, p = 0.04). Patients positive for aCL antibodies had a higher risk of pregnancy loss (OR = 4.62 95% CI, p = 0.001). When these data were tested by multivariate logistic regression, the association between anti-beta 2-GPI and pregnancy loss and the negative association between anti-oxidized LDL antibodies and thrombocytopenia disappeared.  (+info)

Associations of anti-beta2-glycoprotein I autoantibodies with HLA class II alleles in three ethnic groups. (7/19351)

OBJECTIVE: To determine any HLA associations with anti-beta2-glycoprotein I (anti-beta2GPI) antibodies in a large, retrospectively studied, multiethnic group of 262 patients with primary antiphospholipid antibody syndrome (APS), systemic lupus erythematosus (SLE), or another connective tissue disease. METHODS: Anti-beta2GPI antibodies were detected in sera using an enzyme-linked immunosorbent assay. HLA class II alleles (DRB1, DQA1, and DQB1) were determined by DNA oligotyping. RESULTS: The HLA-DQB1*0302 (DQ8) allele, typically carried on HLA-DR4 haplotypes, was associated with anti-beta2GPI when compared with both anti-beta2GPI-negative SLE patients and ethnically matched normal controls, especially in Mexican Americans and, to a lesser extent, in whites. Similarly, when ethnic groups were combined, HLA-DQB1*0302, as well as HLA-DQB1*03 alleles overall (DQB1*0301, *0302, and *0303), were strongly correlated with anti-beta2GPI antibodies. The HLA-DR6 (DR13) haplotype DRB1*1302; DQB1*0604/5 was also significantly increased, primarily in blacks. HLA-DR7 was not significantly increased in any of these 3 ethnic groups, and HLA-DR53 (DRB4*0101) was increased in Mexican Americans only. CONCLUSION: Certain HLA class II haplotypes genetically influence the expression of antibodies to beta2GPI, an important autoimmune response in the APS, but there are variations in HLA associations among different ethnic groups.  (+info)

The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution. (8/19351)

Latrophilin is a brain-specific Ca2+-independent receptor of alpha-latrotoxin, a potent presynaptic neurotoxin. We now report the finding of two novel latrophilin homologues. All three latrophilins are unusual G protein-coupled receptors. They exhibit strong similarities within their lectin, olfactomedin and transmembrane domains but possess variable C-termini. Latrophilins have up to seven sites of alternative splicing; some splice variants contain an altered third cytoplasmic loop or a truncated cytoplasmic tail. Only latrophilin-1 binds alpha-latrotoxin; it is abundant in brain and is present in endocrine cells. Latrophilin-3 is also brain-specific, whereas latrophilin-2 is ubiquitous. Together, latrophilins form a novel family of heterogeneous G protein-coupled receptors with distinct tissue distribution and functions.  (+info)

Zhang Y, Wang L, Zhou W, Wang H, Zhang J, Deng S, Li W, Li H, Mao Z, Ma D. Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervou
TY - JOUR. T1 - Effect of ethanol on the synthesis and secretion of hepatic secretory glycoproteins and albumin. AU - Tuma, D. J.. AU - Jennett, R. B.. AU - Sorrell, Michael Floyd. PY - 1981/1/1. Y1 - 1981/1/1. N2 - The effects of ethanol on the synthesis and secretion of serum glycoproteins and albumin, a nonglycosylated protein, were studied in rat liver slices. Serum glycoproteins and albumin were determined by immunoprecipitation from either the incubation medium or from the washed slices. When ethanol (10 mM) was present in the incubation medium, [14C]glucosamine incorporation in secretory glycoproteins was decreased. This inhibitory effect was, however, much greater in the secretory proteins released into the medium than in those retained in the liver slices. Similar inhibitions by ethanol were also observed when leucine or valine were used as a label for either total export proteins or albumin. Since ethanol impaired protein synthesis, an inhibitor of protein synthesis, cycloheximide, was ...
https://www.dualsystems.com/wp-content/uploads/sites/7/2018/07/2018-Dualsystems-Biotech-Logo-website.png 0 0 helene https://www.dualsystems.com/wp-content/uploads/sites/7/2018/07/2018-Dualsystems-Biotech-Logo-website.png helene2018-07-02 13:19:032018-08-13 16:58:18Leukocyte differentiation by histidine-rich glycoprotein/stanniocalcin-2 complex regulates murine glioma growth through modulation of anti-tumor immunity ...
Stanniocalcin-2 (STC2), the paralog of STC1, has been suggested as a novel target of oxidative stress response to protect cells from apoptosis. The expression of STC2 has been reported to be highly correlated with human cancer development. In this study, we reported that STC2 is a HIF-1 target gene and is involved in the regulation of cell proliferation. STC2 was shown to be up-regulated in different breast and ovarian cancer cells, following exposure to hypoxia. Using ovarian cancer cells (SKOV3), the underlying mechanism of HIF-1 mediated STC2 gene transactivation was characterized. Hypoxia-induced STC2 expression was found to be HIF-1α dependent and required the recruitment of p300 and HDAC7. Using STC2 promoter deletion constructs and site-directed mutagenesis, two authentic consensus HIF-1 binding sites were identified. Under hypoxic condition, the silencing of STC2 reduced while the overexpression of STC2 increased the levels of phosphorylated retinoblastoma and cyclin D in both SKOV3 and MCF7
The ability of PS to enhance the inhibition of FXa by plasma TFPI and by recombinant TFPIα produced in Escherichia coli was originally demonstrated by Hackeng et al.3 Subsequent studies have investigated the interaction of PS and TFPIα in plasma, finding that patients with PS deficiency have decreased plasma TFPIα, that immunodepletion of PS depletes plasma TFPIα as well, and that plasma TFPIα correlates with free PS, rather than with C4bp-bound PS.8 Thus, it seems that there is a physiologically relevant PS-TFPI anticoagulant system functioning in vivo, yet much remains unclear. The majority of intravascular TFPI is TFPIβ on the endothelium, which does not have the PS-binding K3 domain.24,25 In addition, the repercussions of FXa inhibition by PS-TFPIα are not apparent, as TFPIα is a poor inhibitor of thrombin production by the prothrombinase complex assembled with FXa and thrombin-activated FVa, and PS does not enhance this inhibitory function to physiologically relevant rates (Figure ...
Methods and Results-Concomitant with the angiogenic and proliferative responses, hVEGF-DΔNΔC enhanced the phosphorylation of VEGF receptor-2, Akt, and endothelial nitric oxide synthase. Gene arrays, quantitative reverse transcription-polymerase chain reaction, and Western blot revealed increases in VEGF-A, stanniocalcin-1 (STC1), and neuropilin (NRP) 2 expression by hVEGF-DΔNΔC stimulation, whereas induction with hVEGF-A165 altered the expression of STC1 and NRP1, another coreceptor for VEGFs. The effects of hVEGF-DΔNΔC were seen only under high-serum conditions, whereas for hVEGF-A165, the strongest response was observed under low-serum conditions. The hVEGF-DΔNΔC-induced upregulation of STC1 and NRP2 was also evident in vivo in mouse skeletal muscle treated with hVEGF-DΔNΔC by adenoviral gene delivery. The importance of NRP2 in hVEGF-DΔNΔC signaling was further studied with NRP2 small interfering RNA and NRP antagonist, which were able to block hVEGF-DΔNΔC-induced survival of ...
Sigma-Aldrich offers abstracts and full-text articles by [J Rollin, S Iochmann, C Bléchet, F Hubé, S Régina, S Guyétant, E Lemarié, P Reverdiau, Y Gruel].
We used domain-selective biotinylation/125I-streptavidin blotting (Sargiacomo, M., M. P. Lisanti, L. Graeve, A. Le Bivic, and E. Rodriguez-Boulan. 1989 J. Membr. Biol. 107:277-286), in combination with lectin precipitation, to analyze the apical and basolateral glycoprotein composition of Madin-Darby canine kidney (MDCK) cells and to explore the role of glycosylation in the targeting of membrane glycoproteins. All six lectins used recognized both apical and basolateral glycoproteins, indicating that none of the sugar moieties detected were characteristic of the particular epithelial cell surface. Pulse-chase experiments coupled with domain-selective glycoprotein recovery were designed to detect the initial appearance of newly synthesized glycoproteins at the apical or basolateral cell surface. After a short pulse with a radioactive precursor, glycoproteins reaching each surface were biotinylated, extracted, and recovered via precipitation with immobilized streptavidin. Several basolateral ...
Summary The glycoproteins of cells infected with herpes simplex virus 1 or 2 (HSV-1 or HSV-2) were solubilized with non-ionic detergent NP-40 and electrophoresed in acrylamide gels. At least 12 bands containing virus glycoproteins were formed. The HSV-1 glycoproteins correspond in electrophoretic mobility to structural proteins in the HSV virions and to the virus proteins in plasma membranes of infected cells. All virus glycoproteins were bound to immunoadsorbent columns made by cross-linking either homologous or heterologous antibody. Immunoadsorbent columns readily discriminated between host and virus glycoproteins. The differentiation between HSV-1 and HSV-2 glycoproteins varied; the degree of specificity of the immunoadsorbent gels was related to the specificity exhibited by the antisera in neutralization tests. All immunoadsorbent antibody gels tested bound homologous glycoproteins in proportions similar to those in the input extract. Immunoadsorbent antibody gels of high specificity bound
Shop Olfactomedin-like protein ELISA Kit, Recombinant Protein and Olfactomedin-like protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Stanniocalcins (STCs) represent small glycoprotein hormones, found in all vertebrates, which have been functionally implicated in Calcium homeostasis. However, recent data from mammalian systems indicated that they may be also involved in embryogenesis, tumorigenesis and in the context of the latter especially in angiogenesis. Human STC1 is a 247 amino acids protein with a predicted molecular mass of 27 kDa, but preliminary data suggested its di- or multimerization. The latter in conjunction with alternative splicing and/or post-translational modification gives rise to forms described as STC50 and big STC, which molecular weights range from 56 to 135 kDa. In this study we performed a biochemical and structural analysis of STC1 with the aim of obtaining low resolution structural information about the human STC1, since structural information in this protein family is scarce. We expressed STC1 in both E. coli and insect cells using the baculo virus system with a C-terminal 6 × His fusion tag. From the
Sigma-Aldrich offers abstracts and full-text articles by [Jingyuan Su, Bingyu Guo, Tingting Zhang, Kanwen Wang, Xiaoming Li, Guobiao Liang].
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Synonyms for Glycoproteins in Free Thesaurus. Antonyms for Glycoproteins. 10 words related to glycoprotein: mucin, compound protein, conjugated protein, erythropoietin, CD4, cluster of differentiation 4, CD8.... What are synonyms for Glycoproteins?
This is a protein identified originally in the milk fat globule but shown to be widely distributed in epithelial and mesothelial cells. , 1983). , 1983)have been shown to bind with a high degree of selectivity to tissues of neuroectodermal origin. , 1983). A new TAA, Ca 1, was discovered by Ashall et a2. (1982)and identified as a large molecular weight glycoprotein present in the majority of human tumors. , 1983). , 1983b). These workers used a panel of eight mAbs binding to neuroblastoma and five mAbs binding to leukemia and lymphoma cells to diagnose accurately bone marrow aspirates, frozen sections of tumors and cells from malignant effusions, in cases where conventional techniques yielded uncertain results. 1980). Clin. E r p . Zmmuwl. 39, 90-96. Hill, R . , Silburn, P. , Khoo, S. , and Mackay, E. V. (1983). Cynecol. Oncol. 15, 428-433. Hirohashi, S . , and Ino, Y. (1982a). Br. J. Cancer 46, 802-805. , and Ino, Y. (1982b). Gann 73, 345-347. Holmgren, J . , Rudenstam, C. , and Killander, A. ...
Resources available now: This core will provide collaborations, protocols, materials, and advice on: 1) cell surface capture technology: targeted capture and isolation of glycoproteins located on the cell surface (extracellular exposed plasma membrane proteins) of viable cells. 2) The proteomic analysis for glycoprotein enrichment: targeted capture and isolation of glycoproteins from cells, tissues, and body fluids. 3) Assistance in the development of Multiple Reaction Monitoring (MRM) assays: targeted assays for the quantification of specific glycoproteins. (MRM is a cutting edge method for accurately quantifying many different proteins or glycoproteins in a multiplexed manner). 4) Lectin microarrays and lectin-based immunosorbent assays. Additional resources are in the pipeline. ...
The presence of glycoproteins can be assessed in any material by Schiff staining and additional analysis using Western blot, fluorescent microscopy etc. More exact determination of the glycan nature can be accomplished by lectinoblotting using a range of lectins. The identified proteins can be further purified or identified by mass spectrometry. Also, glycan structures can be determined using mass spectrometry. Cells containing glycoproteins can be specifically labelled by Schiff staining or lectins and enriched using a cell sorter.. ...
Although widely distributed in eukaryotic cells glycoproteins appear to be rare in prokaryotic organisms. The prevalence of the misconception that bacteria do not glycosylate their proteins has been a subject matter of discussion for a long time. Glycoconjugates that are linked to proteins or peptid …
It was found previously that transection of one branch of the bifurcate axon of a giant serotonergic neuron of Aplysia causes a proportionate decrease in the export of transmitter storage vesicles from the cell body, despite the ability of the remaining branch to transport all of the vesicles normally exported (Aletta and Goldberg, 1982). We report here evidence that this down-regulation is achieved by a decrease in the production of vesicles, rather than a slowing of the processing of vesicles for export from the cell body. Counts of serotonergic vesicles in the cell body show that the size of the somal pool of vesicles is not increased at a time when their rate of export has been halved. Also, transection of one or both branches of the axon decreases the amount of 3H-fucose incorporated during a short period into a putative vesicle membrane glycoprotein but not into other glycoproteins. The results indicate that axotomy should be a useful technique for studying the mechanism of regulation of ...
The structure of membrane-bound glycoproteins is briefly discussed. Glycoproteins are associated with the cell plasma membrane. Their location on the cell surface has led to suggestions that the...
Glycoproteins are molecules containing sugar and protein, which permit healthy cell-to-cell communication in the human body. Babies receive glycoproteins...
The present invention relates to immunoglobulin glycoprotein compositions having predominant N-glycan structures on an immunoglobulin glycoprotein which confer a specific effector function. Additional
Hourani, B T.; Chace, N M.; and Pincus, J H., Isolation of membrane glycoproteins from l1210 cells with lectin receptor activities. Abstr. (1973). Subject Strain Bibliography 1973. 653 ...
The quantity of enzymes recommended in the protocols is sufficient to deglycosylate approximately 100 μg of an average glycoprotein in the time given. PNGase F cleavage is generally the rate limiting reaction due to the slow removal of some sterically hindered N- linked residues, even when the glycoprotein is denatured. Since all of the enzymes retain activity under reaction conditions for several days, a much larger quantity of glycoprotein may be deglycosylated if incubation is extended. Conversely, there is no need to use the recommended amounts of enzymes if quantities much less than 100 μg of glycoprotein are being cleaved. The enzymes can be diluted into 1X Reaction Buffer 7. They will remain stable in diluted form at 4°C.. Bovine Fetuin Control Protein ...
Each one of these peptides can result in a different VPL motor that can have different properties such as different weight, volume, range of motion, force and speed capabilities. However, the principle of actuation is the same. Studies have shown that the common characteristic in these viruses is the structure of a portion of the surface protein (envelope glycoprotein) and the mode of infection. The envelope glycoproteins of these viruses can be divided into two subunits, which are a result of proteolytic cleavage of a common precursor protein. The two subunits have different functions. For example, in the case of HIV 1 the precursor glycoprotein is gp160, which is proteolytically cleaved into gp120 and gp41 subunits. The gp120 is the surface subunit and the gp41 is the transmembrane (TM) subunit. The surface subunit serves to recognize the cell to be infected when it comes in the vicinity of the virus with the help of receptors located on the cell surface. The gp41 mediates membrane fusion ...
Each one of these peptides can result in a different VPL motor that can have different properties such as different weight, volume, range of motion, force and speed capabilities. However, the principle of actuation is the same. Studies have shown that the common characteristic in these viruses is the structure of a portion of the surface protein (envelope glycoprotein) and the mode of infection. The envelope glycoproteins of these viruses can be divided into two subunits, which are a result of proteolytic cleavage of a common precursor protein. The two subunits have different functions. For example, in the case of HIV 1 the precursor glycoprotein is gp160, which is proteolytically cleaved into gp120 and gp41 subunits. The gp120 is the surface subunit and the gp41 is the transmembrane (TM) subunit. The surface subunit serves to recognize the cell to be infected when it comes in the vicinity of the virus with the help of receptors located on the cell surface. The gp41 mediates membrane fusion ...
GlycoMatrix™ is a new division of bioWORLD. Using our U.S. based manufacturing capability, GlycoMatrix™ has introduced a broad range of the most relevant lectins. Our extensive portfolio contains lectin conjugates for the detection of specific glycoproteins & cancer biomarkers in histological applications, as well
LEGENDplex™ Mouse Cytokine Panel 2 (13-plex) - Cytokines are small soluble glycoproteins that act as extracellular signaling molecuels and meidate communications between cells.
As far as glycoproteins are concerned, at present there is no common international nomenclature. Many investigators have examined them by using various methods of analysis and used more or less...
The carbohydrate protein linkage in glycoproteins. Part I. The synthesis of some model substituted amides and an L -seryl- D -glucosaminide
Semantic Scholar extracted view of [Metabolic disorders of glycoproteins in congenital glycosidase deficiencies]. by K Yamashita
Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chem
Glycoproteins. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
Conventional sample prep techniques for N-glycans can be time-consuming or require compromises in sensitivity. This limits the throughput or detail in which glycoproteins can be characterized or monitored. An innovative label solves those limitations.
1 front of precipitation against rabbit antisera to halpha2-M. Scientific note: Serum glycoprotein of MW approx. 800,000 daltons, containing 38 histidine residues. Two protomers of MW aprox. 370,000 daltons. Each protomer is formed by two sub-units of MW 185,000 daltons, linked by disulfide bridges. N-linked complex carbohydrate side chains ...
Polyclonal antibody for Stanniocalcin 2/STC2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. Stanniocalcin 2/STC2 information: Molecular Weight: 33249 MW; Subcellular Localization: Secreted ; Tissue Specificity:
Mouse Monoclonal Anti-Stanniocalcin 1/STC-1 Antibody (1A3). Validated: WB, ELISA, ICC/IF. Tested Reactivity: Human. 100% Guaranteed.
Dairpoosh wrote: , Im looking for a method for isolation of Glycoproteins , from plant cells.I would be very grateful if someone can provide , me with a method and a refrence. There is no such thing as *the* method to purify proteins, each protein is different and needs its own combination of methods. Now since you are talking about glycoproteins I presume that you are dealing with a transmembrane protein. To isolate those, you homogenise your cells and isolate the compartment where your protein lives (ER, Golgi, PM, lysosome...). There are standard methods for that, check e.g. Meth. Enzymol. Then you solubilise the membranes with a suitable detergent. There are hundreds of detergents out there, dozens are in common use for membrane protein isolation and may be one or two will solubilise your particular protein without destroying its activity. You have to find out which ones. The following is still the first paper to read, despite being 30 years old: @article{Hel-75, AUTHOR= {A. Helenius and K. ...
There is no size limitation for protein that can be purified with the QProteome Glycoprotein Kits as long as the epitope (the glycan structure) of the glycoprotein is accessible for binding to the immobilized lectin in the purification columns ...
Integrins are transmembrane glycoproteins that belong to the family of adhesion molecules. They promote interactions between cells and their…
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Sugar (glucose) is a key molecule used by all cells as fuel to produce bio-energy and it forms the structural part of glycoproteins and other structur...
a glycoprotein on the outer envelope of HIV that binds to the CD4 protein on the surface of certain cells. The V3 loop of gp120 is the principle neutralizing determinant of HIV and is used in some experimental anti-HIV vaccines ...
1997-2006 Healthboard.com. Healthboard.com is a purely informational website, and should not be used as a substitute for professional legal, medical or technical advice. ...
div class=citation vocab=http://schema.org/,,i class=fa fa-external-link-square fa-fw,,/i, Data from ,span resource=http://link.library.missouri.edu/portal/Structural-glycoproteins-in-cell-matrix/lnjJKyQaPhw/ typeof=Book http://bibfra.me/vocab/lite/Item,,span property=name http://bibfra.me/vocab/lite/label,,a href=http://link.library.missouri.edu/portal/Structural-glycoproteins-in-cell-matrix/lnjJKyQaPhw/,Structural glycoproteins in cell-matrix interactions, volume editors, J. Labat-Robert, R. Timpl, L. Robert,/a,,/span, - ,span property=potentialAction typeOf=OrganizeAction,,span property=agent typeof=LibrarySystem http://library.link/vocab/LibrarySystem resource=http://link.library.missouri.edu/,,span property=name http://bibfra.me/vocab/lite/label,,a property=url href=http://link.library.missouri.edu/,University of Missouri Libraries,/a,,/span,,/span,,/span,,/span,,/div ...
TY - JOUR. T1 - Herpes simplex virus glycoproteins gD and gE/gI serve essential but redundant functions during acquisition of the virion envelope in the cytoplasm. AU - Farnsworth, Aaron. AU - Goldsmith, Kimberly. AU - Johnson, David C.. PY - 2003/8/1. Y1 - 2003/8/1. N2 - The late stages of assembly of herpes simplex virus (HSV) and other herpesviruses are not well understood. Acquisition of the final virion envelope apparently involves interactions between viral nucleocapsids coated with tegument proteins and the cytoplasmic domains of membrane glycoproteins. This promotes budding of virus particles into cytoplasmic vesicles derived from the trans-Golgi network or endosomes. The identities of viral membrane glycoproteins and tegument proteins involved in these processes are not well known. Here, we report that HSV mutants lacking two viral glycoproteins, gD and gE, accumulated large numbers of unenveloped nucleocapsids in the cytoplasm. These aggregated capsids were immersed in an ...
SDS-PAGE studies of EBO virion glycoproteins (Fig. 1 and 3A) and N-terminal sequencing have provided definitive evidence that GP is cleaved (from GP0 form) into two molecules, GP1 and GP2. Cleavage of the structural GP gene product of EBO virus was expected for the N-terminal signal sequence and occurs at the predicted site. Cleavage of GP by furin had been predicted from the RRTRR sequence, but identification of the C-terminal fragment in virions was complicated by its comigration with VP24 in SDS-PAGE. However, prior studies detected GP2 in EBO-infected tissue culture fluids (17, 21), and it was suggested that this uncharacterized glycoprotein might be derived from a cleavage event involving GP. Indirect evidence for furin cleavage of EBO virus GP was recently demonstrated through the use of specific inhibitors of furin activity (21).. When GP sequences of all known EBO viruses are aligned, one finds that the furin cleavage site is conserved but differs slightly from one EBO species to ...
Teramoto, Y A. and Schlom, J, Radioimmunoassays for the 36,000-dalton glycoprotein of murine mammary tumor viruses demonstrate type, group, and interspecies determinants. (1979). Subject Strain Bibliography 1979. 4658 ...
Glycoproteins are a very large and biologically relevant class of proteins that comprise more than 50 % of proteins in the human body. The glycosylation present on proteins, specifically N-linked glycosylation has been shown to be important for a variety of processes including protein folding, protein stability, and cell-cell interactions. Many glycoproteins are currently being considered as therapeutic drug targets. Glycosylation on proteins has also been shown to be altered with the onset of diseases, such as cancer, which has opened up the field of glycoproteomics, which aims to detect glycosylation changes for earlier detection of disease states. Mass spectrometry is a versatile technique that is frequently utilized for the analysis of glycoproteins, and it is particularly useful in the detection of glycosylation present on proteins. Most glycoproteins are prepared for mass spectrometric analysis by performing a protease digestion, followed by either a separation by HPLC or some other ...
Fig. 6 OLFML2A and OLFML2B immunoreactivity in human retina. a Confocal image of stained retina sections to identify cells expressing OLFML2A (red, 1st Ab: rabbit polyclonal anti-human OLFML2A 1:500; 2nd Ab: goat anti-rabbit IgG-Cy3® 1:4000). b Confocal image of stained retina seccions to identify cells expressing OLFML2B (red, 1st Ab: rabbit polyclonal anti-human OLFML2B 1:500; 2nd Ab: goat anti-rabbit IgG-Cy3® 1:4000). Cells nuclei were labeled with DAPI (blue). GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer, PL photoreceptor layer. Discussion. Olfactomedin was originally identified as the major component of the mucus layer that surrounds the chemosen-sory dendrites of olfactory neurons [15]. Subsequently, a vast numbers of proteins that share a ~250 amino acid domain homologous to olfactomedin were discovered in animals ranging from nematodes to humans [4]. One of these proteins was the olfactomedin like 2 proteins. (OLFML2A and OLFML2B), also known as photomedins ...
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
gastric mucus glycoproteins: from glandular region of gastric mucosa; high molecular wt. glycoproteins consisting of large numbers of carbohydrate side chains attached to protein core through O-glycosidic linkages; possibly protects stomach from ulceration
The objective of this investigation was primarily to establish that the apparent increase in serum fucose levels in patients with malignant tumors is real and to obtain data as to its source. Carbohydrate residues (L-fucose, D-mannose, D-galactose) were estimated by borate ion-exchange chromatography after mild acid hydrolysis of serum proteins. Data are presented which confirm reported increases in levels of serum fucose in certain malignancies and indicate that these increases may be attributed to quantitative alterations in specific serum glycoproteins observed in the glycoprotein profile associated with cancer. Values for serum fucose levels in pathological and nonpathological sera obtained by various analytical methods are presented and discussed. (Author)
Structure and expression of SPARC (osteonectin, BM-40): a secreted Ca++-binding glycoprotein associated with extracellular matrix production. In: Morphoregulatory Molecules. Eds. Edelman, G.M., Cunnningham, B.A. and Thiery, J.-P ...
Saha, N.,Kamboh, M.I.,Kelly, L.J.,Ferrell, R.E.,Tay, J.S. (1992). Apolipoprotein H (beta-2-glycoprotein I) polymorphism in Asians.. Human Biology 64 (4) : 617-621. [email protected] Repository ...
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Bronner F., Kleinzeller A. Current Topics in Membranes and Transport. Volume 11: Cell Surface Glycoproteins: Structure, Biosynthesis, and Biological Functions (v. 11 ...
INTRODUCTION. In 1999 the Nobel Prize in Physiology or Medicine was awarded to Gunter Blobel for the discovery that proteins have intrinsic signals that govern their transport and localisation in the cell. It was based on this research into how cells use these signals that the functions of glycoproteins were discovered.. Glycoproteins are molecules that have a sugars and protein bonded together. The sugar chains of glycoproteins play a role in determining their destination in the cell or body. The bonding of sugars to proteins to form glycoproteins significantly alters the properties of these proteins.. There are eight sugars that predominantly bond with proteins to form glycoproteins, these are: xylose, galactose, glucose, mannose, fucose, N-acetylgalactosamine, N-acetylglucosamine and N-acetylneuraminic acid.. WHAT ARE THE FUNCTIONS OF GLYCOPROTEINS?. Glycans contain biologic information and are the specific sequence of sugars in a glycoprotein that determine its recognition and interaction ...
Sodium/potassium-transporting ATPase subunit beta-1 is an enzyme that in humans is encoded by the ATP1B1 gene.[1] The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+-ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants ...
Complete information for CETP gene (Protein Coding), Cholesteryl Ester Transfer Protein, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Das APOH Gen kodiert das Apolipoprotein H, welches die Brücke zwischen Lipidstoffwechsel, Koagulation und Infektabwehr schlägt. Es wurde gefunden, dass einige SNPs eine Bedeutung für die Plasmakonzentration besitzen. Damit könnten sie auch einen Effekt auf die prothombotische Wirkung insbesondere bei Patienten mit Phospholipid-Antikörpern besitzen.. ...
View mouse Olfml3 Chr3:103735394-103738001 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
read more] This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is ...
Robert L, Jungua S, Moczar M Structural glycoproteins of the intercellular matrix. The pattern includes a cen- ter circular clear zone and a variable number of incision lines radiating out from the clear zone. ACA ratio The ACA ratio should be measured at 6 m in all patients with intermittent exotropia using the gradi- ent method (see Buy herbal Forzest 5 ). Trans Ophthalmol Buy Sildigra free shipping UK 1985;104146-151.
Buy our Recombinant Stanniocalcin 2 protein. Ab63281 is a full length protein produced in HEK 293 cells and has been validated in WB, ELISA. Abcam provides…
Cross-suppression of AREG/EREG expression may explain the tight co-expression of AREG and EREG, as well as their tendency to be more highly expressed than other EGFR ligands to determine Ctx efficacy. The positive selection for Ctx-resistant tumour cells exhibiting AREG/EREG cross-suppression may ha …
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Glycoproteins are a type of peptide naturally present in skins surface that works with other ingredients to reinforce skins barrier strength.
シビアな構造異常糖タンパク質が出現すると手順を踏まずに強制分解 -酵母にはない高等動物特有の小胞体糖タンパク質分解システムの解明-. 京都大学プレスリリース. 2015-11-20.
Reference: Kuzmak N.I., Panasiuk E.N., Effect of stress on concentration of blood glycoproteins in rats with different functional changes in the thyroid gland, Voprosy meditsinskoi khimii, 1982, vol: 28(3), 89-94 ...
Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.
In structural biology, a protomer is the structural unit of an oligomeric protein. It is the smallest unit composed of at least two different protein chains that form a larger hetero-oligomer by association of two or more copies of this unit. The term was introduced by Chetverin [1] to make nomenclature in the Na/K-ATPase enzyme unambiguous. This enzyme is composed of two subunits, a large catalytic subunit, alpha and a smaller glycoprotein subunit, beta (plus a proteolipid, called γ-subunit). At the time it was unclear how many of each work together. In addition, when people spoke of a dimer, did they refer to αβ or to (αβ)2? Chetverin suggested to call αβ a protomer and (αβ)2 a diprotomer. Protomers usually arrange in cyclic symmetry to form closed point group symmetries. In chemistry, a so-called protomer is a molecule which displays tautomerism due to position of a proton.[2][3] ...
Our research focuses on the chemical synthesis and the biological. investigation of signaling molecules for cells. We are developing. and improving syntheses for three classes of biomolecules:. oligosaccharides, peptides and glycoproteins. Oligosaccharides. are playing a major role in cell-surface binding events, protein. stability and immunology and can be considered an emerging field. in bioscience. Oligosaccharides have potential as diagnostic markers. and vaccines and can be synthesized by chemical and enzymatic. methods. Proteins and glycoproteins are of high interest for biotechnology. since their recombinant production provides many pharmaceutical. products such as cellular growth factors or monoclonal antibodies.. ...
Gentaur molecular products has all kinds of products like :search , FabGennix \ Olfactomedin 1, WB control \ PC-Olf for more molecular products just contact us
Description: CDCP1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 646 amino acids (30-667a.a.) and having a molecular mass of 72.8kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). CDCP1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques ...
The goal of the proposed research is to develop a general and highly efficient chemo enzymatic method for making diverse homogeneous glycopeptides and glycoprot...
CD33 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain(18-259 a.a)fused to a 6 aa His Tag at C-terminus containing a total of 484 aa.
Scientists have developed a system which can pinpoint potential biomarkers of early forms of cancer, by looking at structures of specific sugar molecules which are attached either to proteins of cancerous cells or to proteins involved in the host response. The availability of such cancer biomarkers could also allow disease progression and response to therapy to be monitored more easily and accurately than is currently possible.
SLAMF7 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 212 amino acids (23-226a.a.) and having a molecular mass of 23.4kDa.
Biology of Reproduction contains original scientific research on a broad range of topics in the field of reproductive biology, as well as minireviews.
Background The MHC molecules are glycoproteins encoded in a large cluster of genes located on chromosome 6. They were first identified by their potent
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Gamblin, David P.; Scanlan, Eoin M.; Davis, Benjamin G. (2009). "Glycoprotein Synthesis: An Update". Chemical Reviews. 109 (1 ... Davis, B. G. (2002). "Synthesis of glycoproteins". Chemical Reviews. 102 (2): 579-602. doi:10.1021/cr0004310. PMID 11841255. ...
The major glycoproteins of the egg coat responsible, are known as sperm-binding proteins. The four major sperm-binding proteins ... ZP module-containing glycoproteins ZP1, ZP2, ZP3 and ZP4 are targets for immunocontraception in mammals. In non-mammals, the ... The zona pellucida (plural zonae pellucidae, also egg coat or pellucid zone) is a glycoprotein layer surrounding the plasma ... The zona pellucida is a translucent matrix of glycoproteins that surrounds the mammalian oocyte, and its formation is critical ...
They are glycoproteins. Then you have the signal. They stimulate release of pituitary hormones. They stimulate synthesis of ...
Zosquidir inhibits P-glycoproteins. Other drugs with this mechanism include tariquidar and laniquidar. P-glycoproteins are ... Cancers overexpressing P-glycoproteins are able to pump out therapeutic molecules before they are able to reach their target, ... Zosuquidar inhibits P-glycoproteins, inhibiting the efflux pump and restoring sensitivity to chemotherapeutic agents. Zosuqidar ...
Glycoproteins are integral proteins. They play an important role in the immune response and protection. The phospholipid ... they can be covalently bound to lipids to form glycolipids or covalently bound to proteins to form glycoproteins. Membranes ...
Perez-Vilar J, Hill RL (2004). "Mucin Family of Glycoproteins". Encyclopedia of Biological Chemistry (Lennarz & Lane, EDs.). ... Western Blotting for High-Molecular-Weight Glycoproteins". J Vis Exp. 112: 54153. doi:10.3791/54153. PMC 4927784. PMID 27341489 ...
... p-glycoproteins MeSH D12.776.395.550.020.610.610 - p-glycoprotein MeSH D12.776.395.550.200.065 - antigens, cd22 MeSH D12.776. ... platelet glycoprotein gpib-ix complex MeSH D12.776.395.550.625.785 - platelet glycoprotein gpiib-iiia complex MeSH D12.776. ... This list covers glycoproteins. For other protein-related codes, see List of MeSH codes (D12.776). Codes before these are found ... 395.550.625.800 - receptors, thrombin MeSH D12.776.395.550.625.800.780 - platelet glycoprotein gpib-ix complex MeSH D12.776. ...
One mechanism to explain this may come from its ability to bind to the extracellular matrix glycoprotein fibronectin and block ... Jones F, Jones P (2000). "The tenascin family of ECM glycoproteins: structure, function, and regulation during embryonic ... Tenascins are extracellular matrix glycoproteins. They are abundant in the extracellular matrix of developing vertebrate ...
Harding, S. E.; Creeth, J. M. (1983-07-28). "Polyelectrolyte behaviour in mucus glycoproteins". Biochimica et Biophysica Acta ( ... and glycoproteins such as lactoferrin and mucins, which are produced by goblet cells in the mucous membranes and submucosal ... glycoproteins), proteoglycans (0.1% -0.5%), lipids (0.3% - 0.5%), proteins, and DNA. The major mucins secreted - MUC5AC, and ...
There are also 11 glycoproteins. These are gB, gC, gD, gE, gG, gH, gI, gJ, gK, gL and gM. Tegument contains 26 proteins. They ... There are spikes made of glycoprotein protruding from each virion. These can expand the diameter of the virus to 225 nm. The ... The structural components of a typical HSV virion are the Lipid bilayer envelope, Tegument, DNA, Glycoprotein spikes and ... Following binding of viral envelope glycoproteins to cell membrane receptors, the virion is internalized and dismantled, ...
Glycoproteins[edit]. Glycoproteins have distinct Oligosaccharide structures which have significant effects on many of their ... Glycoproteins are relevant as cell-surface receptors, cell-adhesion molecules, immunoglobulins, and tumor antigens.[12] ... Glycoproteins and glycolipids are by definition covalently bonded to carbohydrates. They are very abundant on the surface of ... 1 All cells are coated in either glycoproteins or glycolipids, both of which help determine cell types.[7] Lectins, or proteins ...
He developed the synthesis of glycoproteins. In addition, Brik was involved in projects related to the discovery of inhibitors ... "Sugar-Assisted Ligation in Glycoprotein Synthesis", Journal of the American Chemical Society, American Chemical Society, 129 ( ...
Their glycoproteins are rich in mannose. The cell wall might have evolved to deter viral infections. Proteins embedded in cell ... Algae possess cell walls made of glycoproteins and polysaccharides such as carrageenan and agar that are absent from land ... A third type of wall among the Archaea consists of glycoprotein, and occurs in the hyperthermophiles, Halobacterium, and some ... The cell walls of archaea have various compositions, and may be formed of glycoprotein S-layers, pseudopeptidoglycan, or ...
Both these receptors are transmembrane glycoproteins. TfR1 is a high affinity ubiquitously expressed receptor while expression ...
When glycoproteins are not broken down, aspartylglucosaminidase backs up in the lysosomes along with other substances. This ... Aspartylglucosaminidase functions to break down glycoproteins. These proteins are most abundant in the tissues of the body and ... glycoproteins). Aspartylglucosaminuria itself is characterized as a lysosomal disease because it does deal with inadequate ...
ISG: inversion-specific glycoprotein. FLA: fasciclin like AGP. ENODL: earlt nodulation like. MF: male fertility. TTS: ... Hallmann, A.; Kirk, D. L. (December 2000). "The developmentally regulated ECM glycoprotein ISG plays an essential role in ... Cheung, Alice Y; Wang, Hong; Wu, Hen-ming (August 1995). "A floral transmitting tissue-specific glycoprotein attracts pollen ... Arabinogalactan-proteins (AGPs) are highly glycosylated proteins (glycoproteins) found in the cell walls of plants. Each one ...
... of different chemical nature have been obtained, including polysaccharides, glycoproteins, peptides and ... "Chemoenzymatic synthesis of oligosaccharides and glycoproteins". Trends in Biochemical Sciences. 29 (12): 656-663. doi:10.1016/ ...
"Glycoprotein glycan profiling". academic.oup.com. Retrieved November 22, 2019. CS1 maint: discouraged parameter (link) Mechref ... Comparison of the methods for profiling glycoprotein glycans-HUPO Human Disease Glycomics/Proteome Initiative multi- ...
"Myelin-associated Glycoprotein". Molecule of the Month. RCSB PDB. July 2020. Nelson M, Chazin W. "Home Page for Calmodulin". EF ...
Witte, Krista; Sears, Pamela; Martin, Richard; Wong, Chi-Huey (1997-03-01). "Enzymatic Glycoprotein Synthesis: Preparation of ... Sears, P. (2001-03-23). "Toward Automated Synthesis of Oligosaccharides and Glycoproteins". Science. 291 (5512): 2344-2350. ... Sears, P. (2001-03-23). "Toward Automated Synthesis of Oligosaccharides and Glycoproteins". Science. 291 (5512): 2344-2350. ... Sears, P. (2001-03-23). "Toward Automated Synthesis of Oligosaccharides and Glycoproteins". Science. 291 (5512): 2344-2350. ...
The cadherins are homophilic Ca2+ -dependent glycoproteins. The classic cadherins (E-, N- and P-) are concentrated at the ... which is a mucin-type glycoprotein expressed on all white blood cells. Selectins have been implicated in several roles but they ... The best-characterized ligand for the three selectins is P-selectin glycoprotein ligand-1 (PSGL-1), ...
Glycoproteins have differential levels of glycosylations and adsorb SDS more unevenly at the glycosylations, resulting in ... Buxbaum, Engelbert (2003). "Cationic electrophoresis and electrotransfer of membrane glycoproteins". Analytical Biochemistry. ...
The nature of N-linked glycans attached to a glycoprotein is determined by the protein and the cell in which it is expressed. ... The synthesis of glycoproteins is thus spatially separated in different cellular compartments. Therefore, the type of N-glycan ... The removal of the final third glucose residue signals that the glycoprotein is ready for transit from the ER to the cis-Golgi ... There are two types of bonds involved in a glycoprotein: bonds between the saccharides residues in the glycan and the linkage ...
Each strain of the influenza virus has a different pattern of glycoproteins; the glycoproteins themselves have variability as ... It has a protein membrane containing the glycoproteins hemagglutinin (HA) and neuraminidase (NA) which are used by the virus to ... Krammer F (May 2017). "Strategies to induce broadly protective antibody responses to viral glycoproteins". Expert Review of ... School of Medicine in St.Louis and the Icahn School of Medicine in Mount Sinai in New York are using the glycoprotein ...
In particular, he believed that testing glycoproteins could detect early-stage cancer. In 1959, Paleček received a PhD in ... Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics". Chemical Reviews. ...
Online Mendelian Inheritance in Man (OMIM): 609241 Cantz M, Ulrich-Bott B (1990). "Disorders of glycoprotein degradation". J. ... "Schindler disease". International Advocate for Glycoprotein Storage Diseases. (International Society for Mannosidosis & Related ... which leads to excessive lysosomal accumulation of glycoproteins. A deficiency of the alpha-NAGA enzyme leads to an ... as they have been successful in curing other glycoprotein disorders.[citation needed] Schindler disease was named after Detlev ...
AT is a glycoprotein. It has 86% carbohydrate content. It has been reported that the maturation of AT is a stepwise process ...
Harpaz, N; Schachter, H (1980). "Control of glycoprotein synthesis. Processing of asparagine-linked oligosaccharides by one or ... Schachter also helped characterize the first Carbohydrate-Deficient Glycoprotein Syndrome (CDG-IIa; now known as Congenital ... "Mutations in the MGAT2 gene controlling complex N-glycan synthesis cause carbohydrate-deficient glycoprotein syndrome type II, ... thereby defining processes crucial for the formation of branched oligosaccharides on glycoproteins that include cell surface ...
It is a glycoprotein. Although it is not enzymatically active, it is structurally related to several serine proteases of the ... a vitamin K-dependent plasma glycoprotein". Biochem. Biophys. Res. Commun. 172 (3): 1139-44. doi:10.1016/0006-291X(90)91566-B. ...
... is a database of experimentally verified glycosites and glycoproteins of the prokaryotes. glycoprotein Bhat, Aadil ... a repository of experimentally characterized prokaryotic glycoproteins". Nucleic Acids Res. England. 40 (Database issue): D388- ...
Membrane glycoproteins are membrane proteins which play important roles in cell recognition. Examples include: Fibronectin ... Laminin Osteonectin Glycocalyx Media related to Membrane glycoproteins at Wikimedia Commons Membrane+glycoproteins at the US ...
Glycoprotein-41 (gp41) and glycoprotein-120 (gp120) are HIV viral coat proteins. Soluble glycoproteins often show a high ... Glycoproteins are important for white blood cell recognition.[citation needed] Examples of glycoproteins in the immune system ... Other examples of glycoproteins include: gonadotropins (luteinizing hormone a follicle-stimulating hormone) glycoprotein IIb/ ... One example of glycoproteins found in the body is mucins, which are secreted in the mucus of the respiratory and digestive ...
In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that ... Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who ... Case Studies in Mass Spectrometry of Glycoproteins. * Front Matter Pages 277-277 ... Characterizing the Glycosylation State of Therapeutic Recombinant Glycoproteins Nicole Samuels, David Kates, Jun Liu, Joanne ...
glycoprotein. glycoprotein glī˝kōprō´tēn [key], organic compound composed of both a protein and a carbohydrate joined together ... One set of glycoproteins also carry the blood group determinants. The carbohydrate portion of a glycoprotein is usually a small ... Salivary mucus contains the glycoprotein called mucin. Among other glycoproteins, one particularly interesting example is ... Solutions of glycoproteins usually exhibit high viscosity, an observation explaining the highly viscous character of egg white ...
Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. It was discovered by ... Human Immunodeficiency Virus Glycoprotein 120 Vashistha H, Husain M, Kumar D, Singhal PC (2009). "Tubular cell HIV-1 gp120 ... A conserved region in the gp120 glycoprotein that is involved in the metastable attachment of gp120 to CD4 has been identified ... Gp120 is anchored to the viral membrane, or envelope, via non-covalent bonds with the transmembrane glycoprotein, gp41. Three ...
... Mauricio Cesar Palmieri mcpalmie at FOX.CCE.USP.BR Fri Apr 8 07:26:47 EST 1994 *Previous message: How do you ...
Words related to glycoproteins:. = 48 && event.charCode <= 57 value="Num letters..." onfocus="inputFocus(this)" onblur=" ...
Structure of the chlorovirus PBCV-1 major capsid glycoprotein determined by combining crystallographic and carbohydrate ...
Although glycoproteins and proteoglycans have been a subject of re- search for many years, it is only during the last five or ... Although glycoproteins and proteoglycans have been a subject of re- search for many years, it is only during the last five or ... Cross section Mammalia biological functions biosynthesis cell chemistry development glycoprotein immunoglobulin interferon ... The reason for this expanded interest in these molecules is simple: not only are glycoproteins and proteoglycans ubiq- uitous, ...
Although widely distributed in eukaryotic cells glycoproteins appear to be rare in prokaryotic organisms. The prevalence of the ... Most studied prokaryotic glycoproteins are the S-layer glycoproteins of Archeabacteria. Apart from these, membrane-associated, ... surface-associated, secreted glycoproteins and exoenzymes glycoproteins are also well documented in both, Archea and Eubacteria ... Bacterial glycoproteins: functions, biosynthesis and applications Proteomics. 2003 Apr;3(4):363-79. doi: 10.1002/pmic.200390052 ...
Glycoproteins are usually found at the surface of cells and assist with important processes ... Glycoproteins are a form of protein that contain sugar residue. ... N-linked Glycoproteins. These types of glycoproteins are ... It then attaches other sugars so that it forms new glycoproteins.. O-linked Glycoproteins. These types of glycoproteins are ... This extracellular matrix surrounds the O-linked glycoproteins.. Non-enzymatic Glycoproteins. This creates glycoproteins when ...
Glycoprotein VI deficiency is a bleeding disorder associated with a decreased ability to form blood clots. Explore symptoms, ... Glycoprotein VI deficiency can be caused by mutations in the GP6 gene, which provides instructions for making a protein called ... Some cases of glycoprotein VI deficiency are not caused by GP6 gene mutations; instead these cases are acquired, which means ... The prevalence of glycoprotein VI deficiency is unknown. At least 15 cases have been described in the scientific literature. ...
Dairpoosh wrote: , Im looking for a method for isolation of Glycoproteins , from plant cells.I would be very grateful if ... Glycoprotein. Dr Engelbert Buxbaum via methods%40net.bio.net (by engelbert_buxbaum from hotmail.com). Fri Apr 6 10:17:07 EST ... Now since you are talking about glycoproteins I presume that you are dealing with a transmembrane protein. To isolate those, ...
Structure and biosynthesis of prokaryotic glycoproteins.. Lechner J1, Wieland F.. Author information. 1. Department of ...
The HIV envelope glycoprotein is a challenging protein to study by X-ray crystallography as it sits in a membrane, it is ... Because the envelope glycoprotein is critical for infection, it is an obvious target for HIV therapy and thus the subject of ... Ebola glycoprotein, a previous featured structure, serves the same function to recognise host cells and internalise the virus. ... If the body can produce antibodies to the envelope glycoprotein, the virus can be prevented from binding to, and infecting ...
Amyloidogenic glycoprotein, extracellular (IPR008154). Short name: Amyloid_glyco_extra Overlapping homologous superfamilies * ... This entry represents an extracellular domain that is usually found at the N-terminal of amyloidogenic glycoproteins such as ...
... so it is unclear which glycan is associated with the function of the glycoprotein. Moreover, because glycoproteins are ... homogenous glycoprotein has been prepared by using a chemoenzymatic approach to investigate the function of glycoproteins. ... To solve this problem, transgenic cells have been developed to produce homogeneous glycoproteins through control of the ... Because antibody-dependent cell-mediated cytotoxicity (ADCC) exists as an example of glycan function in glycoproteins, there ...
Glycoproteins are molecules containing sugar and protein, which permit healthy cell-to-cell communication in the human body. ... You can find glycoproteins in many fruits, from apples and pears to oranges and other citrus fruits. Pectins, often found in ... Glycoproteins are also found in aloe vera, bran, oatmeal, barley, brown rice, wheat, red wine and meat. Echinacea and turmeric ... Babies receive glycoproteins from their mothers milk that hinder bacteria, viruses and even cancer cells. For those who want ...
Therefore we have developed a cell-free expression system for the synthesis of glycoproteins. Using a Pichia pastoris cell ... Our results indicate the successful in vitro synthesis of a glycoprotein with a GlcNAc2Man5 glycan. The GlcNAc2Man5 core ...
Purchase Glycoprotein and Proteoglycan Techniques, Volume 16 - 1st Edition. Print Book & E-Book. ISBN 9780444806529, ... Glycoprotein and Proteoglycan Techniques, Volume 16 1st Edition. 0.0 star rating Write a review ... 2. Glycoproteins and proteoglycans. 3. Isolation and fractionation. 4. Physico-chemical characterisation. 5. Analysis of ... The past two decades have seen an expansion of interest in glycoproteins. From being a borderline area between carbohydrate and ...
Recombinant Glycoprotein Expression. Product Listing Application Overview Recombinant expression of glycoproteins is of great ... Recombinant Glycoprotein Expression includes these areas of focus: Expression Systems. FAQs for Recombinant Glycoprotein ... Yeast primarily modifies glycoproteins with only high mannose N-glycans.. *Plant and insect cell lines can modify N-glycans ... Some mammalian cell lines can add the non-human epitopes Gal α1-3 Gal or N-glycolylneuraminic acid (NGNA) to glycoproteins. ...
... , P-Glycoprotein Inhibitor, P-Glycoprotein Substrate, P-Glycoprotein Inducer, P-gp. ... P Glycoproteins, P-Glycoproteins, P GLYCOPROTEIN Z, P-Glycoproteins [Chemical/Ingredient], p glycoproteins, p-glycoprotein, p- ... P-Glycoprotein. Aka: P-Glycoprotein, P-Glycoprotein Inhibitor, P-Glycoprotein Substrate, P-Glycoprotein Inducer, P-gp ... P-Glycoprotein [Chemical/Ingredient], p-glycoprotein, p glycoprotein, p-gp, glycoprotein p, ATP-Binding Cassette, Sub-Family B ...
Glycoprotein Ib-IX-V complex. The GPIb-IX-V complex is an abundant membrane receptor complex originating in megakaryocytes and ... University of NottinghamResearchGroupsStructural BiologyResearch projectsGlycoprotein GP Ib IX-V ...
The monoclonal antibody has a continuous amino acid sequence, and a binding affinity for the P-glycoprotein which manifests in ... A monoclonal antibody that recognises a structurally continuous and extracellularly-located epitope of human P-glycoprotein is ... Glycoprotein P is a plasma membrane protein which is over-expressed on the surfaces of cell lines or human tumour cells that ... The monoclonal antibody has a continuous amino acid sequence, and a binding affinity for the P-glycoprotein which manifests in ...
... column packed with amide 1.7 lm sorbent was applied to the characterization of glycoprotein digests. Due to the impact of the ... Characterization of glycoprotein digests with hydrophilic interaction chromatography and mass spectrometry. *Gilar M ... column packed with amide 1.7 lm sorbent was applied to the characterization of glycoprotein digests. Due to the impact of the ...
What is P-Glycoprotein?. P-glycoprotein (P-gp) is a plasma membrane protein which acts as a localized drug transport mechanism ...
Glycoprotein glycosylation and cancer progression.. Dennis JW1, Granovsky M, Warren CE. ... Glycosylation of glycoproteins and glycolipids is one of many molecular changes that accompany malignant transformation. GlcNAc ... beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase. *alpha-1,6-mannosylglycoprotein beta 1,6-N- ...
... are a type of peptide naturally present in skins surface that works with other ingredients to reinforce skins ... Glycoproteins help other peptides be more soluble in water and within skin, so some products add them along with specific ... On skin, glycoproteins work with ingredients like glycerin, ceramides, and hyaluronic acids to keep skin intact, smooth, and ... "Glyco" means "sugar", so glycoproteins are a type of protein linked to a sugar. Theyre considered a type of peptide and are ...
patterns, provoking an immune response when treating humans with GPs produced in plants. State of the art approaches are based either on glycosylation mutants or overproduction of the desired protein product (intending to override the cellular glycosylation machinery). Though the mutants either show impaired yield or loss of vitality in comparison to wild type, or the proteins do not terminate in mannose residues, which is essential for the biological uptake via mannose receptors in patients with lysosomal storage diseases. This invention provides a new device to generate GPs with hypo-allergenic properties in a cost-effective way: In contrast to current methods, the new technique enables effective production of heterologous GPs by vital plants without impairments. The technology is based on a genetic modification of the N-glycosylation pathway. It is applicable to Solanaceous and other plants of agronomical interest as transgenic producers of GPs. The suppression of a specific enzyme leads to a ...
  • Membrane glycoproteins are membrane proteins which play important roles in cell recognition. (wikipedia.org)
  • Glycoproteins are proteins which contain oligosaccharide chains (glycans) covalently attached to amino acid side-chains. (wikipedia.org)
  • Carbohydrates are attached to some proteins to form glycoproteins. (wikipedia.org)
  • Glycoproteins are also often important integral membrane proteins, where they play a role in cell-cell interactions. (wikipedia.org)
  • Monosaccharides commonly found in eukaryotic glycoproteins include: The sugar group(s) can assist in protein folding, improve proteins' stability and are involved in cell signalling. (wikipedia.org)
  • Glycoprotein-41 (gp41) and glycoprotein-120 (gp120) are HIV viral coat proteins. (wikipedia.org)
  • Many proteins released by cells to the blood and other fluids are glycoproteins. (infoplease.com)
  • P-glycoproteins are plasma membrane proteins containing two ATP-binding sites and twelve putative transmembrane segments. (nih.gov)
  • Electron microscopy is better placed to study proteins with these characteristics and the image in our calendar is based on the cryoEM map of the envelope glycoprotein solved by Sriram Subramaniam's group. (ebi.ac.uk)
  • A subfamily of transmembrane proteins from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS that are closely related in sequence to P-GLYCOPROTEIN. (fpnotebook.com)
  • Although P-Glycoproteins share functional similarities to MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS they are two distinct subclasses of ATP-BINDING CASSETTE TRANSPORTERS, and have little sequence homology. (fpnotebook.com)
  • Arenaviruses are comprised of two RNA genome segments and four proteins, the polymerase L, the envelope glycoprotein GP, the matrix protein Z, and the nucleoprotein NP. (mdpi.com)
  • Beta-2 glycoprotein antibody is considered one of the primary autoantibodies called antiphospholipid antibodies that mistakenly target the body's own lipid-proteins (phospholipids) found in the outermost layer of cells (cell membranes) and platelets. (labtestsonline.org)
  • Glycoproteins are proteins that have covalently attached sugar residues. (blogspot.com)
  • Glycoproteins are usually secreted proteins that normally function outside the cell. (blogspot.com)
  • The first 295 amino acids of both proteins are identical in the Zaire strain, but secreted glycoprotein (sGP) contains an additional 69 and the transmembrane glycoprotein (GP) another 381 COOH-terminal amino acid residues ( 6 ). (sciencemag.org)
  • Myelin Oligodendrocyte Glycoprotein (MOG) is one of the proteins in myelin , a fatty insulating substance found in the white matter of the central nervous system . (mult-sclerosis.org)
  • Here, we will review our knowledge on the glycoprotein biogenesis and the role of Gn and Gc proteins in the phlebovirus replication cycle. (mdpi.com)
  • Glycoproteins are proteins that contain oligosaccharide chains ( glycans ) covalently attached to their polypeptide backbones. (wikidoc.org)
  • CandyCane glycoprotein molecular weight standards separate into alternating bands of glycosylated and nonglycosylated proteins during polyacrylamide gel electrophoresis. (thermofisher.com)
  • These cells become visible around d 19-20 of gestation in cattle and have been shown to secret a plethora of hormones and proteins, including placental lactogen (PL) and pregnancy associated glycoproteins (PAGs). (ivis.org)
  • A conserved region in the gp120 glycoprotein that is involved in the metastable attachment of gp120 to CD4 has been identified and targeting of invariant region has been achieved with a broadly neutralising antibody, IgG1-b12. (wikipedia.org)
  • Because antibody-dependent cell-mediated cytotoxicity (ADCC) exists as an example of glycan function in glycoproteins, there have been many studies of glycan remodeling of antibodies. (go.jp)
  • A monoclonal antibody that recognises a structurally continuous and extracellularly-located epitope of human P-glycoprotein is described. (google.com)
  • 1. A monoclonal antibody that recognizes a structurally continuous and extracellularly-located epitope of human P-glycoprotein consisting of a continuous amino acid sequence, and which has a binding affinity for P-glycoprotein such that it is capable of staining greater than 90% of live CEM-VBL10 cells when tested in a flow cytometry experiment. (google.com)
  • 2. A monoclonal antibody of claim 1 in which the said amino acid sequence is located on the fourth extracellular loop of human P-glycoprotein. (google.com)
  • 9. A method of claim 8 in which a peptide corresponding to a structurally continuous extracellular domain of human P-glycoprotein or a part thereof is used to screen for the desired monoclonal antibody. (google.com)
  • 11. An immunological diagnostic kit containing as specific reagent a monoclonal antibody according to either of claims 1, 2, 3, 4, 5, 6, or 7 for the detection of human multidrug-resistant cells or human P-glycoprotein. (google.com)
  • Beta-2 glycoprotein 1 antibody is an autoantibody that is associated with inappropriate blood clotting. (labtestsonline.org)
  • A Fab fragment of the chimeric monoclonal antibody 7E3 and inhibitor of the glycoprotein (GP) IIb/IIIa receptor, that blocks platelet aggregation. (fpnotebook.com)
  • 2. The method of claim 1, wherein the glycoprotein comprises an antibody or an Fc-fusion protein. (freepatentsonline.com)
  • citation needed] Examples of glycoproteins in the immune system are: molecules such as antibodies (immunoglobulins), which interact directly with antigens. (wikipedia.org)
  • If the body can produce antibodies to the envelope glycoprotein, the virus can be prevented from binding to, and infecting human cells. (ebi.ac.uk)
  • Vaccines could aid in raising antibodies, but unfortunately, the envelope glycoprotein is extremely variable due to the high mutation rate of the virus, and also masked by extensive glycosylation, both features which make raising antibodies difficult. (ebi.ac.uk)
  • There are many structures in the PDB of fragments of glycoprotein bound to antibodies, in efforts to understand the their interaction. (ebi.ac.uk)
  • A recombinant glycoprotein E (gE) from varicella-zoster virus (VZV) was generated and produced in Chinese Hamster Ovary (CHO) cells, in the development of a specific antigen for analysis of IgG antibodies to VZV. (gu.se)
  • The present invention relates to monoclonal antibodies which have specificity for P-glycoprotein (Pgp). (google.com)
  • This test detects and measures beta-2 glycoprotein 1 antibodies in the blood. (labtestsonline.org)
  • Listed below are anti-Envelope glycoprotein antibodies from multiple suppliers. (biocompare.com)
  • Your search returned 223 envelope glycoprotein Antibodies across 13 suppliers. (biocompare.com)
  • Therefore, secondly, we aim to generate murine monoclonal antibodies to define the immunological hierarchy of the antigenic sites of phylogroup 2 glycoproteins. (jcvi.org)
  • Intended for the in vitro quantitative measurement of antibodies directed to β2-Glycoprotein I in human serum and plasma to aid in the diagnosis of antiphospholipid syndrome (APS) and to evaluate the thrombotic risk in patients with systemic lupus erythematosus (SLE). (fishersci.com)
  • If present in the patient's specimen, antibodies to β2-Glycoprotein I bind to their specific antigen. (fishersci.com)
  • One of the three structural glycoproteins of classical swine fever virus (CSFV) is E0, a disulfide-bonded homodimer that induces virus-neutralizing antibodies and occurs in a virion-bound as well as a secreted form. (sciencemag.org)
  • Elevated intrathecal myelin oligodendrocyte glycoprotein antibodies in multiple sclerosis. (biomedsearch.com)
  • OBJECTIVE: To evaluate antibodies to myelin oligodendrocyte glycoprotein (MOG) in the serum and cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) and control individuals. (biomedsearch.com)
  • Gp120 is anchored to the viral membrane, or envelope, via non-covalent bonds with the transmembrane glycoprotein, gp41. (wikipedia.org)
  • Now since you are talking about glycoproteins I presume that you are dealing with a transmembrane protein. (bio.net)
  • The protein is an ATP-dependent, transmembrane transport/efflux pump glycoprotein that is expressed in liver, kidney, small intestine and brain. (fpnotebook.com)
  • A 170-kDa transmembrane glycoprotein from the superfamily of ATP-BINDING CASSETTE TRANSPORTERS. (fpnotebook.com)
  • P-gp is a 170 kDa transmembrane glycoprotein , which includes 10-15 kDa of N-terminal glycosylation. (wikipedia.org)
  • The mechanisms by which Ebola virus evades detection and infects cells to cause hemorrhagic fever have not been defined, though its glycoprotein, synthesized in either a secreted or transmembrane form, is likely involved. (sciencemag.org)
  • Here the secreted glycoprotein was found to interact with neutrophils through CD16b, the neutrophil-specific form of the Fc γ receptor III, whereas the transmembrane glycoprotein was found to interact with endothelial cells but not neutrophils. (sciencemag.org)
  • A murine retroviral vector pseudotyped with the transmembrane glycoprotein preferentially infected endothelial cells. (sciencemag.org)
  • Thus, the secreted glycoprotein inhibits early neutrophil activation, which likely affects the host response to infection, whereas binding of the transmembrane glycoprotein to endothelial cells may contribute to the hemorrhagic symptoms of this disease. (sciencemag.org)
  • Among the viral gene products, the glycoprotein is found in two forms: a secreted form, 50 to 70 kD ( 6 ), synthesized in large amounts early in the course of infection, and an alternative transmembrane form, which arises from RNA editing to encode a 120- to 150-kD glycoprotein that is incorporated into the virion ( 6 , 7 ). (sciencemag.org)
  • Ebola glycoprotein, a previous featured structure , serves the same function to recognise host cells and internalise the virus. (ebi.ac.uk)
  • Jeremy Luban, Aaron Lin, and Ted Diehl join the TWiV team to discuss their work on identifying a single amino acid change i n the Ebola virus glycoprotein from the West African outbreak that increases infectivity in human cells. (virology.ws)
  • Ebola virus glycoprotein Fc fusion protein confers protection against lethal challenge in vaccinated mice" Vaccine 2011 Apr. (fda.gov)
  • To determine the specificity of Ebola virus glycoproteins, we transfected expression vectors encoding either sGP, GP, or a plasmid control ( 8 ) into human 293 cells, and cell culture supernatants were used as a source of relevant recombinant glycoproteins. (sciencemag.org)
  • Ebola virus (EBOV) entry requires the surface glycoprotein (GP) to initiate attachment and fusion of viral and host membranes. (shapeways.com)
  • Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. (wikipedia.org)
  • Because the envelope glycoprotein is critical for infection, it is an obvious target for HIV therapy and thus the subject of much research. (ebi.ac.uk)
  • The HIV envelope glycoprotein is a challenging protein to study by X-ray crystallography as it sits in a membrane, it is heavily glycosylated and conformationally heterogenous. (ebi.ac.uk)
  • At the centre of the envelope glycoprotein are the three important and conserved helices. (ebi.ac.uk)
  • Pasquato, A. Envelope Glycoprotein of Arenaviruses. (mdpi.com)
  • The envelope glycoproteins of herpes simplex virus 1 (HSV-1) and HSV-2, with the exception of glycoprotein G, elicit cross-reactive B- and T-cell responses. (gu.se)
  • Macrophages express low levels of CD4, and macrophage-tropic HIV strains express envelope glycoproteins (Envs) adapted to overcome this restriction to virus entry by mechanisms that are not well characterized. (harvard.edu)
  • Membrane glycoproteins from influenza viruses which are involved in hemagglutination, virus attachment, and envelope fusion. (harvard.edu)
  • N-linked, glycosylation can prevent proper glycoprotein folding and full inhibition can be toxic to an individual cell. (wikipedia.org)
  • N-linked protein glycosylation (N-glycosylation of N-glycans) at Asn residues (Asn-x-Ser/Thr motifs) in glycoproteins. (wikipedia.org)
  • This creates glycoproteins when sugar is added to polypeptides in a process called glycosylation. (reference.com)
  • On the other hand, glycosylation is a pre-requisite for proper protein folding, which in case of diagnostic and therapeutic glycoproteins should ideally not include immu-nogenic residues. (innovations-report.com)
  • Perturbation of ER-associated functions such as disturbed ER glycoprotein quality control, protein glycosylation and protein folding results in activation of an ER stress coping response. (mdpi.com)
  • In addition, glycoproteins of viruses such as the human immunodeficiency virus (HIV) contain various N-glycosylation sites, which may aid in shielding the virus from immune system recognition. (medium.com)
  • The exposure of peptide backbone structures of mucin glycoproteins in malignancies appears to be due to abnormal glycosylation during biosynthesis. (springer.com)
  • Plants have shown to be an excellent production host for a variety of glycoproteins with the added benefit of possibilities to engineer the N-glycosylation pathway. (wur.nl)
  • It is a glycoprotein with a 42-kDa core protein with extensive N-linked glycosylation. (atlasgeneticsoncology.org)
  • and informatics tools used for identifying glycoproteins and characterizing the associated glycans. (springer.com)
  • Glycoproteins produced by cultured cells generally have heterogeneous glycans, so it is unclear which glycan is associated with the function of the glycoprotein. (go.jp)
  • Yeast primarily modifies glycoproteins with only high mannose N-glycans. (neb.com)
  • Structural characteristics of glycoproteins, in general, and the correlation between certain compositional patterns of their glycans and the nature of the tissue elements with which they are associated are first discussed and demonstrated in a certain number of cases. (arvojournals.org)
  • On the other hand, it is pointed out that there appears to be a correlation between the ratio of the end groups of these glycans to each other and to the backbone constituent and functional properties of the glycoproteins. (arvojournals.org)
  • There were also significant differences between the three regions of lenses of adult rabbits as far as the ratio of neuraminic acid to fucose in the glycans of their glycoproteins is concerned. (arvojournals.org)
  • In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. (springer.com)
  • Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry. (springer.com)
  • Then we washed the dendrimer off with a low pH solution, and we had the glycoproteins analyzed with things like mass spectrometry,' Wu said. (news-medical.net)
  • The study showed that CD205, a trans membrane glycoprotein , is robustly expressed in a variety of solid malignancies, such as pancreatic and bladder tumors, as well as triple negative breast cancer (TNBC). (thefreedictionary.com)
  • The IHC study of CD-99 (a cell membrane glycoprotein ) and intranuclear FLI-1 (a DNA-binding transcription factor) is important to diagnose ES. (thefreedictionary.com)
  • NASDAQ: RCKT), is developing its first adeno-associated viral vector -based gene therapy, RP-A501, that is designed to restore the lysosome-associated membrane glycoprotein 2 (LAMP-2) gene which is defective in patients afflicted with Danon disease, the company said. (thefreedictionary.com)
  • Lattice structures forming between LacNAc repeats on N-glycan branches, and galectins determine plasma membrane glycoprotein residency time by inhibiting endocytosis of them (31,34). (thefreedictionary.com)
  • At the beginning of this century, a number of researchers conducted systematic reviews on the association of platelet membrane glycoprotein polymorphisms and risk of CAD. (thefreedictionary.com)
  • Platelet glycoprotein 4 (CD36) (or fatty acyl translocase [FAT], or scavenger receptor class B, member 3 [SCARB3]) is an essential cell surface and skeletal muscle outer mitochondrial membrane glycoprotein involved in multiple functions in the body. (mdpi.com)
  • Other examples of glycoproteins include: gonadotropins (luteinizing hormone a follicle-stimulating hormone) glycoprotein IIb/IIIa, an integrin found on platelets that is required for normal platelet aggregation and adherence to the endothelium. (wikipedia.org)
  • and the evolution of the versatile and prolific P-glycoprotein gene family. (nih.gov)
  • Glycoprotein VI deficiency can be caused by mutations in the GP6 gene, which provides instructions for making a protein called glycoprotein VI (GPVI). (medlineplus.gov)
  • Human p-glycoprotein-1 (1280 aa, 141 kDa) is encoded by the human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1) gene. (fpnotebook.com)
  • P-gp is a glycoprotein that in humans is encoded by the ABCB1 gene. (wikipedia.org)
  • glycoprotein glī˝kōprō´tēn [ key ] , organic compound composed of both a protein and a carbohydrate joined together in covalent chemical linkage. (infoplease.com)
  • The carbohydrate portion of a glycoprotein is usually a small sugar or no more than 8 to 10 individual monosaccharide units. (infoplease.com)
  • A new family of cognate glycoproteins with chitinase-sensitive carbohydrate moiety, chitinoproteins (ChiP) has been detected in Drosophila melanogaster and other insects. (europa.eu)
  • A series of chaperones, folding enzymes, glucosidases, and carbohydrate transferases support glycoprotein synthesis and processing. (mdpi.com)
  • The Wittmann Group uses metabolic glycoengineering techniques to accomplish the integration of functional groups into the carbohydrate portion - glycan - of a glycoprotein. (medium.com)
  • In epithelial cancers, many of the phenotypic markers for pre-malignant and malignant cells have been found on the carbohydrate and peptide moieties of mucin glycoproteins. (springer.com)
  • Basically, glycoprotein is a biomolecule composed of a protein and a carbohydrate (an oligosaccharide). (wikidoc.org)
  • The first chapter deals primarily with structural work on the oligosaccharide chains of glycoproteins, but it will be apparent in it and in the succeeding two chapters on biosynthesis that not only do structural studies aid biosynthetic investigations, but that studies on biosynthesis often playa major role in elucidation of structure. (springer.com)
  • Structure and biosynthesis of prokaryotic glycoproteins. (nih.gov)
  • Overexpression of P-glycoprotein genes is a well-established cause of one form of multidrug resistance. (nih.gov)
  • Overexpression of this glycoprotein is associated with multidrug resistance (see DRUG RESISTANCE, MULTIPLE). (fpnotebook.com)
  • this obstacle is mainly due to the overexpression of P-glycoprotein (P-gp). (ingentaconnect.com)
  • From the aggressive prostate cancer tissues, we identified the overexpression of three glycoproteins involved in an extracellular matrix remodeling and further examined two glycoproteins, cathepsin L and periostin, using Western blot and immunohistochemistry analyses. (nih.gov)
  • it gives a good, broad perspective on the variable and yet ordered structures shown by glycoproteins and proteoglycans, and a thorough chemical overview of what can and cannot be done to the intact glycoproteins and their isolated glycopeptides. (elsevier.com)
  • Once the dendrimer caught a glycoprotein, the researchers used a magnet to grab the bead and pull out their chemical octopus along with its ensnared glycopeptides (e.g. glycoproteins). (news-medical.net)
  • To purify glycoproteins or glycopeptides that bind the particular lectin used. (wikidoc.org)
  • glycoprotein IIb/IIIa , an integrin found on platelets that is required for normal platelet aggregation and adherence to the endothelium . (wikipedia.org)
  • Platelet glycoprotein VI-related clinical defects. (medlineplus.gov)
  • Eptifibatide selectively and reversibly binds to and blocks the platelet glycoprotein IIb/IIIa receptor. (fpnotebook.com)
  • Holmes, R.S. Comparative Studies of Vertebrate Platelet Glycoprotein 4 (CD36). (mdpi.com)
  • In addition to these specific glycoprotein standards, Sigma offers a broad variety of glycoproteins for analytical and biological use as controls and bioactive components. (sigmaaldrich.com)
  • P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp ) also known as multidrug resistance protein 1 ( MDR1 ) or ATP-binding cassette sub-family B member 1 ( ABCB1 ) or cluster of differentiation 243 ( CD243 ) is an important protein of the cell membrane that pumps many foreign substances out of cells. (wikipedia.org)
  • A 2015 review of polymorphisms in ABCB1 found that "the effect of ABCB1 variation on P-glycoprotein expression (messenger RNA and protein expression) and/or activity in various tissues (e.g. the liver, gut and heart) appears to be small. (wikipedia.org)
  • ATP-binding cassette (ABC) transporters, in particular P-glycoprotein (encoded by ABCB1), are important and selective elements of the blood-brain barrier (BBB), and they actively contribute to brain homeostasis. (hindawi.com)
  • P-glycoprotein (P-gp/Abcb1), Abcc2, and Abcc3 determine the pharmacokinetics of etoposide. (webmd.com)
  • Babies receive glycoproteins from their mothers' milk that hinder bacteria, viruses and even cancer cells. (livestrong.com)
  • Found in dengue virus and many related viruses, dimers of these viral glycoproteins lie flat on the viral membrane, and are comprised largely of beta-strands. (virology.ws)
  • Furthermore, vesicular stomatitis viruses with HAP2 in place of the viral glycoprotein cannot enter cells. (virology.ws)
  • Salivary mucus contains the glycoprotein called mucin. (infoplease.com)
  • At present, the role of mucin glycoproteins in various stages of epithelial cell carcinogenesis (including the preneoplastic state and metastasis), in cancer diagnosis and immunotherapy is under investigation. (springer.com)
  • The aim of this study was to evaluate the effect of gefarnate on mucin-like glycoprotein secretion in isolated rabbit conjunctival tissue, and on corneal epithelial damage in rabbit and cat dry-eye models. (dovepress.com)
  • Mucin-like glycoprotein was detected in the culture supernatant by an enzyme-linked lectin assay. (dovepress.com)
  • Gefarnate stimulated mucin-like glycoprotein secretion in conjunctival tissue in a dose-dependent manner. (dovepress.com)
  • These results suggest that gefarnate stimulates in vitro secretion of mucin-like glycoprotein in conjunctival tissue and ameliorates corneal epithelial damage in animal dry-eye models. (dovepress.com)
  • To solve this problem, transgenic cells have been developed to produce homogeneous glycoproteins through control of the biosynthetic pathway, but it is difficult to prepare glycoproteins with various kinds of homogeneous glycan by using this approach. (go.jp)
  • Our results indicate the successful in vitro synthesis of a glycoprotein with a GlcNAc2Man5 glycan. (aiche.org)
  • Data are presented which confirm reported increases in levels of serum fucose in certain malignancies and indicate that these increases may be attributed to quantitative alterations in specific serum glycoproteins observed in the glycoprotein profile associated with cancer. (dtic.mil)
  • Synthetic peptide within Human P Glycoprotein (C terminal). (abcam.com)
  • The preparation and analysis of glycoproteins of the lens of cattle and rabbits is then described, in three parts of the lens, namely, the equatorial, the polar, and the nuclear region. (arvojournals.org)
  • Therefore we have developed a cell-free expression system for the synthesis of glycoproteins. (aiche.org)
  • Multidrug resistance mediated by P-glycoproteins. (nih.gov)
  • Reversal of P-glycoprotein-mediated multidrug resistance and phar. (ingentaconnect.com)
  • The removal of one or more alpha 1,2-linked mannose residues from a mannosylated protein that occurs as part of glycoprotein ER-associated glycoprotein degradation (gpERAD). (yeastgenome.org)
  • Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore. (freepatentsonline.com)
  • In comparison tests with existing methods, the chemical trap, a complex molecular construction reminiscent of an octopus, captured exponentially more glycoproteins, especially more of those trace glycoproteins. (news-medical.net)
  • How to trace glycoproteins in living cells? (medium.com)
  • Juliano R.L., Ling V.A. A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. (wikidoc.org)
  • Many people make sure to include glycoproteins in their diet as a way to boost their immune systems and prevent or delay the spread of cancer. (livestrong.com)
  • It is helpful and often necessary to include glycoproteins as standards and/or molecular weight markers. (sigmaaldrich.com)
  • Evolutionary disparate Antarctic notothenioids and Arctic gadids have adapted to their freezing environments through the elaboration of essentially identical antifreeze glycoproteins (AFGPs). (cambridge.org)
  • Antifreeze glycoproteins not lost with the faeces are resorbed from the gut via the rectal epithelium, transported to the blood and ultimately secreted into the bile, from where they re-enter the gastrointestinal tract. (cambridge.org)
  • Antarctic notothenioid fishes and several northern cods are phylogenetically distant (in different orders and superorders), yet produce near-identical antifreeze glycoproteins (AFGPs) to survive in their respective freezing environments. (pnas.org)
  • The most striking example of evolution of the same type of antifreeze protein in unrelated fishes is the near-identical antifreeze glycoproteins (AFGPs) in the Antarctic notothenioid fishes and several northern cods of the family Gadidae ( 8 , 9 ). (pnas.org)
  • structural glycoproteins, which occur in connective tissue. (wikipedia.org)
  • Most studied prokaryotic glycoproteins are the S-layer glycoproteins of Archeabacteria. (nih.gov)
  • The knowledge pertaining to the functional aspects of prokaryotic glycoproteins is rather scarce. (nih.gov)
  • This review summarizes developments and understanding relating to characteristics, synthesis, and functions of prokaryotic glycoproteins. (nih.gov)
  • One of these, M1536-B3, synthesizes and deposits copious quantities of extracellular matrix glycoproteins, which led to the initial discovery and characterization of laminin and entactin. (biomedsearch.com)
  • Some mammalian cell lines can add the non-human epitopes Gal α1-3 Gal or N-glycolylneuraminic acid (NGNA) to glycoproteins. (neb.com)
  • Recombinant glycoprotein E produced in mammalian cells in large-scale as an antigen for varicella-zoster-virus serology. (gu.se)
  • [8] In 2009, the first structure of a mammalian P-glycoprotein was solved (3G5U). (wikipedia.org)
  • Glycoproteins change the plasma membrane permeability making it easier for the attraction of eggs to the sperm cells. (reference.com)
  • P-glycoprotein (permeability glycoprotein , abbreviated as P-gp or Pgp ) is a well-characterized ABC-transporter of the MDR / TAP subfamily. (thefullwiki.org)
  • Induction of lipolysis in vitro and loss of body fat in vivo by zinc-alpha2-glycoprotein. (anabolicminds.com)
  • Although widely distributed in eukaryotic cells glycoproteins appear to be rare in prokaryotic organisms. (nih.gov)
  • The TWiVirions reveal bacteriophage genes that control eukaryotic reproduction, and the biochemical basis for increased Ebolavirus glycoprotein activity during the recent outbreak. (virology.ws)
  • Mucins are high molecular weight glycoproteins that are heavily glycosylated with many oligosaccharide side chains linked O-glycosidically to the protein backbone. (springer.com)
  • Recombinant expression of glycoproteins is of great interest, particularly in the production of therapeutic glycoproteins such as immunoglobulins and hormones for the biopharmaceutical market. (neb.com)
  • P-glycoprotein (abbreviated as P-gp or Pgp ) is a well characterized human ABC-transporter of the MDR / TAP subfamily. (wikidoc.org)
  • The reason for this expanded interest in these molecules is simple: not only are glycoproteins and proteoglycans ubiq- uitous, but many are molecules with well-defined and important biological functions. (springer.com)
  • Glycoproteins are molecules containing sugar and protein, which permit healthy cell-to-cell communication in the human body. (livestrong.com)
  • Glycoproteins are protein molecules bonded with sugar molecules, and they're very common in all living things. (news-medical.net)
  • Existing methods to reel in glycoproteins for laboratory examination are relatively new and have had big holes in their nets, so many of these molecules, especially those very rare ones produced by cancer, have tended to slip by. (news-medical.net)
  • Soluble glycoproteins often show a high viscosity, for example, in egg white and blood plasma. (wikipedia.org)
  • Solutions of glycoproteins usually exhibit high viscosity, an observation explaining the highly viscous character of egg white, which is composed largely of the glycoprotein ovalbumin. (infoplease.com)
  • Many secreted glycoproteins are inserted into the outer membrane of the cell. (blogspot.com)
  • The protein part of the glycoprotein is created on the surface by other amino acids. (reference.com)
  • Synthetic peptide corresponding to a region within amino acids 249 - 311 of Human Oligodendrocyte myelin glycoprotein. (abcam.com)
  • Eight sugars commonly found in glycoproteins. (wikipedia.org)
  • The carbohydrates that are inside the glycoprotein are modified by enzymes that also takes away some of the sugars. (reference.com)
  • It then attaches other sugars so that it forms new glycoproteins. (reference.com)
  • As a general rule, a bunch of these sugars are strung together to form an oligosaccharide chain (see below) and it's this long chain that's attached to the protein to form a glycoprotein. (blogspot.com)
  • The eight sugars contained in glycoproteins. (wikidoc.org)
  • Identifies sugars that the glycoprotein contains and their stoichiometry. (wikidoc.org)
  • P-glycoprotein has also recently been designated CD243 ( cluster of differentiation 243). (thefullwiki.org)
  • There are many methods utilized in glycoprotein detection and identification, with the most common being SDS-PAGE, mass spectroscopy, HPLC, NMR and Western blotting. (sigmaaldrich.com)
  • Researchers at the Georgia Institute of Technology have engineered a chemical trap that exhaustively catches what are called glycoproteins , including minuscule traces that have previously escaped detection. (news-medical.net)
  • Antifreeze glycoprotein recirculation conserves energy expenditure and explains how high levels of AFGPs reach the blood in notothenioids since, unlike Arctic gadids which also synthesize AFGP in the liver, AFGP secretion in notothenioids is directed exclusively towards the gastrointestinal lumen. (cambridge.org)
  • Among other glycoproteins, one particularly interesting example is isolated from certain antarctic fishes who survive near-freezing water temperatures as a result of freezing-point depression of their blood serum by a globular glycoprotein. (infoplease.com)
  • This entry represents an extracellular domain that is usually found at the N-terminal of amyloidogenic glycoproteins such as amyloid-beta precursor protein (APP, or A4). (ebi.ac.uk)
  • Researchers at FDA developed an efficacious Filovirus subunit vaccine based on a recombinant protein consisting of the extracellular domain of the Filovirus glycoprotein fused to an Fc Fragment of human immunoglobulin (FiloGP-Fc). (fda.gov)
  • One example of glycoproteins found in the body is mucins, which are secreted in the mucus of the respiratory and digestive tracts. (wikipedia.org)
  • Quantitative glycoproteomic analysis of optimal cutting temperature-embedded frozen tissues identifying glycoproteins associated with aggressive pr. (nih.gov)
  • Glycoproteins help other peptides be more soluble in water and within skin, so some products add them along with specific peptides as a way to enhance delivery and stability of the formula. (paulaschoice.com)
  • Glycoproteins are usually found at the surface of cells and assist with important processes in the body. (reference.com)
  • Glycoproteins are usually found at the surface of cells. (reference.com)
  • Glycoproteins aid in the development of tissue function when they adhere to the cells found in the body. (reference.com)
  • Cancer cells that have too much p-glycoprotein may not be killed by anticancer drugs. (fpnotebook.com)
  • The FiloGP-Fc fusion protein resembles the native glycoprotein expressed at the surface of cells and viral particles. (fda.gov)
  • Myelin and myelin oligodendrocyte glycoprotein are produced by maintenance cells called oligodendrocytes . (mult-sclerosis.org)