Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
The characteristic 3-dimensional shape of a carbohydrate.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
A proteolytic enzyme obtained from Streptomyces griseus.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
Glycopeptide antibiotic complex from Actinoplanes teichomyceticus active against gram-positive bacteria. It consists of five major components each with a different fatty acid moiety.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
Antibacterial obtained from Streptomyces orientalis. It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
SUGARS containing an amino group. GLYCOSYLATION of other compounds with these amino sugars results in AMINOGLYCOSIDES.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
The N-acetyl derivative of galactosamine.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Substances that reduce the growth or reproduction of BACTERIA.
Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
The N-acetyl derivative of glucosamine.
A beta-N-Acetylhexosaminidase that catalyzes the hydrolysis of terminal, non-reducing 2-acetamido-2-deoxy-beta-glucose residues in chitobiose and higher analogs as well as in glycoproteins. Has been used widely in structural studies on bacterial cell walls and in the study of diseases such as MUCOLIPIDOSIS and various inflammatory disorders of muscle and connective tissue.
A heterogeneous mixture of glycoproteins responsible for the gel structure of egg white. It has trypsin-inhibiting activity.
An amidohydrolase that removes intact asparagine-linked oligosaccharide chains from glycoproteins. It requires the presence of more than two amino-acid residues in the substrate for activity. This enzyme was previously listed as EC 3.2.2.18.
A group of related enzymes responsible for the endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose-content glycopeptides and GLYCOPROTEINS.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A family of calcium-binding alpha-globulins that are synthesized in the LIVER and play an essential role in maintaining the solubility of CALCIUM in the BLOOD. In addition the fetuins contain aminoterminal cystatin domains and are classified as type 3 cystatins.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.
Enzymes that catalyze the joining of two molecules by the formation of a carbon-oxygen bond. EC 6.1.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
A species of STAPHYLOCOCCUS found on the skin of humans (and non-human primates), often causing hospital-acquired infections (CROSS INFECTION).
A strong oxidizing agent.
The sum of the weight of all the atoms in a molecule.
Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with an alcohol to form an acetal. They include both alpha- and beta-mannosides.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Enzymes that catalyze the hydrolysis of N-acylhexosamine residues in N-acylhexosamides. Hexosaminidases also act on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES.
Infections caused by bacteria that retain the crystal violet stain (positive) when treated by the gram-staining method.
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
The systematic study of the structure and function of the complete set of glycans (the glycome) produced in a single organism and identification of all the genes that encode glycoproteins.
Nonsusceptibility of bacteria to the action of VANCOMYCIN, an inhibitor of cell wall synthesis.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
Infections with bacteria of the genus STAPHYLOCOCCUS.
Carbohydrate antigen elevated in patients with tumors of the breast, ovary, lung, and prostate as well as other disorders. The mucin is expressed normally by most glandular epithelia but shows particularly increased expression in the breast at lactation and in malignancy. It is thus an established serum marker for breast cancer.
An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)
Endogenous glycoproteins from which SIALIC ACID has been removed by the action of sialidases. They bind tightly to the ASIALOGLYCOPROTEIN RECEPTOR which is located on hepatocyte plasma membranes. After internalization by adsorptive ENDOCYTOSIS they are delivered to LYSOSOMES for degradation. Therefore receptor-mediated clearance of asialoglycoproteins is an important aspect of the turnover of plasma glycoproteins. They are elevated in serum of patients with HEPATIC CIRRHOSIS or HEPATITIS.
A hydroxylated derivative of the amino acid LYSINE that is present in certain collagens.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
An enzyme that oxidizes galactose in the presence of molecular oxygen to D-galacto-hexodialdose. It is a copper protein. EC 1.1.3.9.
Derivatives of acetamide that are used as solvents, as mild irritants, and in organic synthesis.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A system of universal human blood group isoantigens with many associated subgroups. The M and N traits are codominant and the S and s traits are probably very closely linked alleles, including the U antigen. This system is most frequently used in paternity studies.
A species of gram-positive, coccoid bacteria whose organisms are normal flora of the intestinal tract. Unlike ENTEROCOCCUS FAECALIS, this species may produce an alpha-hemolytic reaction on blood agar and is unable to utilize pyruvic acid as an energy source.
A subclass of anhydrides with the general structure of dihydrofurandione. They can be substituted on any carbon atom. They modify and inhibit proteins and enzymes and are used in the acylation of amino- and hydroxyl groups.
Derivatives of oxazolidin-2-one. They represent an important class of synthetic antibiotic agents.
Non-susceptibility of a microbe to the action of METHICILLIN, a semi-synthetic penicillin derivative.
An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Inorganic and organic derivatives of sulfuric acid (H2SO4). The salts and esters of sulfuric acid are known as SULFATES and SULFURIC ACID ESTERS respectively.
Proteins that bind to ice and modify the growth of ice crystals. They perform a cryoprotective role in a variety of organisms.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Bacteria which retain the crystal violet stain when treated by Gram's method.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The first alpha-globulins to appear in mammalian sera during FETAL DEVELOPMENT and the dominant serum proteins in early embryonic life.

Glycopeptides from the surgace of human neuroblastoma cells. (1/2148)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Crystal structure of an MHC class I presented glycopeptide that generates carbohydrate-specific CTL. (2/2148)

T cell receptor (TCR) recognition of nonpeptidic and modified peptide antigens has been recently uncovered but is still poorly understood. Immunization with an H-2Kb-restricted glycopeptide RGY8-6H-Gal2 generates a population of cytotoxic T cells that express both alpha/beta TCR, specific for glycopeptide, and gamma/delta TCR, specific for the disaccharide, even on glycolipids. The crystal structure of Kb/RGY8-6H-Gal2 now demonstrates that the peptide and H-2Kb structures are unaffected by the peptide glycosylation, but the central region of the putative TCR binding site is dominated by the extensive exposure of the tethered carbohydrate. These features of the Kb/RGY8-6H-Gal2 structure are consistent with the individual ligand binding preferences identified for the alpha/beta and gamma/delta TCRs and thus explain the generation of a carbohydrate-specific T cell response.  (+info)

Crystal structures of two H-2Db/glycopeptide complexes suggest a molecular basis for CTL cross-reactivity. (3/2148)

Two synthetic O-GlcNAc-bearing peptides that elicit H-2Db-restricted glycopeptide-specific cytotoxic T cells (CTL) have been shown to display nonreciprocal patterns of cross-reactivity. Here, we present the crystal structures of the H-2Db glycopeptide complexes to 2.85 A resolution or better. In both cases, the glycan is solvent exposed and available for direct recognition by the T cell receptor (TCR). We have modeled the complex formed between the MHC-glycopeptide complexes and their respective TCRs, showing that a single saccharide residue can be accommodated in the standard TCR-MHC geometry. The models also reveal a possible molecular basis for the observed cross-reactivity patterns of the CTL clones, which appear to be influenced by the length of the CDR3 loop and the nature of the immunizing ligand.  (+info)

The structure of a glycopeptide (GP-II) isolated from Rhizopus saccharogenic amylase. (4/2148)

Mild alkaline treatment of glycopeptide (GP-II) resulted in the loss of 1 mole of serine and 5 moles of threonine per mole of GP-II, suggesting the presence of O-glycosyl bonds between 1 serine and 5 threonine residues and carbohydrate chains. Treatment of GP-II with alkaline borohydride released only disaccharide. Methylation studies of the carbohydrate moiety gave 2,3,4,6-tetra-O-methyl and 2,4,6-tri-O-methyl derivatives of mannose in a ratio of approximately 1:1. In addition, one step of Smith degradation resulted in the loss of about 6 residues of mannose per mole of GP-II. Moreover, alpha-mannosidase [EC 3.2.1.24] liberated about 6 residles of mannose per mole of GP-II. On the basis of these data, the structure of the carbohydrate moiety of GP-II was confirmed to be 3-O-alpha-mannosylmannose. The amino- and carboxyl-terminal amino acids of GP-II were determined to be threonine and serine, respectively. On reductive cleavage of N-proline bonds with metallic sodium in liquid ammonia, 2 moles of alanine per mole of GP-II were lost. From the compositions of three fragments isolated from the reductive cleavage products, the amino acid sequence of the peptide portion of GP-II was determined. Based on these data, a probable structure was proposed for GP-II.  (+info)

Participation of a trisaccharide-lipid in glycosylation of oviduct membrane glycoproteins. (5/2148)

Preincubation of a hen oviduct membrane preparation with UDP-Nactyl[14C]glucosamine and bacitracin, followed by incubation with GDP-mannose, leads to formation of a chloroform/methanol (2/1)-extractable glycolipid. Treatment of the lipid with mild acid results in the release of a trisaccharide shown to have the structure beta-mannosyl-N-acetylglucosamineyl-N-acetylglucosamine. Incubation of purified trisaccharide-lipid with oviduct membranes in the presence of sodium deoxycholate, Mn2+, and GDP-mannose leads to formation of a labeled glycoprotein with an apparent molecular weight of 25,000...  (+info)

Synergy of an investigational glycopeptide, LY333328, with once-daily gentamicin against vancomycin-resistant Enterococcus faecium in a multiple-dose, in vitro pharmacodynamic model. (6/2148)

The pharmacodynamics of an investigational glycopeptide, LY333328 (LY), alone and in combination with gentamicin, against one vancomycin-susceptible and two vancomycin-resistant Enterococcus faecium strains were studied with a multiple-dose, in vitro pharmacodynamic model (PDM). Dose-range data for the PDM studies were obtained from static time-kill curve studies. In PDM experiments conducted over 48 h, peak LY concentrations of 0.1x and 1x the MIC every 24 h and peak gentamicin concentrations of 18 micrograms/ml every 24 h (Gq24 h) and 6 micrograms/ml every 8 h (Gq8 h) were studied alone and in the four possible LY-gentamicin combinations. Compared to either antibiotic alone, LY-gentamicin combination regimens produced significantly higher apparent killing rates (KRs) calculated during the initial 2 h postdosing. The mean KRs for LY or gentamicin alone versus those for the LY-gentamicin combination regimens were 0.35 +/- 0.55 log10 CFU/ml/h (95% confidence interval [CI95%], 0 to 0.70) and 1.46 +/- 0.71 log10 CFU/ml/h (CI95%, 1.01 to 1.91), respectively (P < 0.0001). Bacterial killing at 48 h (BK48), which was calculated by subtracting the bacterial counts at 48 h from the initial inoculum, with a negative value indicating net growth, was also significantly greater. The mean BK48S were -0.69 +/- 0.44 log10 CFU/ml (CI95%, -0.41 to -0.97) and 3.72 +/- 2.28 log10 CFU/ml (CI95%, 2.28 to 5.17) for LY or gentamicin alone versus LY-gentamicin combination regimens, respectively (P < 0.0001). None of the 12 regimens with LY or gentamicin alone but 75% (9 of 12) of the LY-gentamicin combination regimens were bactericidal. Eighty-three percent (10 of 12) of the LY-gentamicin combination regimens also demonstrated synergy. No significant differences between the pharmacodynamics of LY-gentamicin combination regimens containing Gq24 h versus those containing Gq8h were detected.  (+info)

Evaluation of bactericidal activities of LY333328, vancomycin, teicoplanin, ampicillin-sulbactam, trovafloxacin, and RP59500 alone or in combination with rifampin or gentamicin against different strains of vancomycin-intermediate Staphylococcus aureus by time-kill curve methods. (7/2148)

This in vitro study evaluated the activities of vancomycin, LY333328, and teicoplanin alone and in combination with gentamicin, rifampin, and RP59500 against Staphylococcus aureus isolates with intermediate susceptibilities to vancomycin. Ampicillin-sulbactam and trovafloxacin were also evaluated. LY333328 and ampicillin-sulbactam resulted in bactericidal activity against all isolates. The combination of gentamicin with glycopeptides showed synergistic activity, while rifampin had no added benefit.  (+info)

Evidence for an adenovirus type 2-coded early glycoprotein. (8/2148)

We have identified an adenovirus type 2 (Ad2)-induced early glycopolypeptide with an apparent molecular weight of 20,000 to 21,000 (20/21K), as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The 20/21K polypeptide could be labeled in vivo with [(3)H]glucosamine. [(35)S]methionine- and [(3)H]-glucosamine-labeled 20/21K polypeptides bound to concanavalin A-Sepharose columns and were eluted with 0.2 M methyl-alpha-d-mannoside. The pulse-labeled polypeptide appeared as a sharp band with an apparent molecular weight of 21K, but after a chase it converted to multiple bands with an average molecular weight of 20K. This variability in electrophoretic mobility is consistent with glycosylation or deglycosylation of the 20/21K polypeptide. Analysis of the pulse and pulse-chase-labeled forms by using partial proteolysis indicated that the polypeptides were highly related chemically, but not identical. Most of the 20/21K polypeptide is localized in the cytoplasm fraction of infected cells lysed by Nonidet P-40. The 20/21K polypeptide and a 44K polypeptide, labeled with [(35)S]methionine or [(3)H]glucosamine in Ad2-infected human cells, were precipitated by a rat antiserum against an Ad2-transformed rat cell line (T2C4), but not by antisera against three other Ad2-transformed rat cell lines, or by serum from nonimmune rats. The partial proteolysis patterns of the 20/21K and the 44K polypeptides were indistinguishable, indicating that the two polypeptides are highly related, and suggesting that the 44K polypeptide might be a dimer of the 20/21K polypeptide. The 20/21K polypeptide was also induced in Ad2-early infected monkey and hamster cells. These results imply that the 20/21K polypeptide is synthesized in Ad2-infected human, monkey, and hamster cells, and in one but not all Ad2-transformed rat cells. Thus, the 20/21K polypeptide is probably viral coded rather than cell coded and viral induced.  (+info)

This page is Related Information of Characterization of Glycan Binding Site of O-Linked Glycopeptides Using MALDI-7090 High-Resolution MALDI-TOF MS.
A meta-analysis was performed to investigate whether a switch from β-lactams to glycopeptides for cardiac surgery prophylaxis should be advised. Results of 7 randomized trials (5761 procedures) that compared surgical site infections (SSIs) in subjects receiving glycopeptide prophylaxis with SSIs in those who received β-lactam prophylaxis were pooled. Neither agent proved to be superior for prevention of the primary outcome, occurrence of SSI at 30 days (risk ratio [RR], 1.14; 95% confidence interval [CI], 0.91-1.42). In subanalyses, β-lactams were superior to glycopeptides for prevention of chest SSIs (RR, 1.47; 95% CI, 1.11-1.95) and approached superiority for prevention of deep-chest SSIs (RR, 1.33; 95% CI, 0.91-1.94) and SSIs caused by gram-positive bacteria (RR, 1.36; 95% CI, 0.98-1.91). Glycopeptides approached superiority to β-lactams for prevention of leg SSIs (RR, 0.77; 95% CI, 0.58-1.01) and were superior for prevention of SSIs caused by methicillin-resistant gram-positive bacteria ...
TY - JOUR. T1 - One-Step Enrichment of Intact Glycopeptides From Glycoengineered Chinese Hamster Ovary Cells. AU - Yang, Ganglong. AU - Höti, Naseruddin. AU - Chen, Shao Yung. AU - Zhou, Yangying. AU - Wang, Qiong. AU - Betenbaugh, Michael. AU - Zhang, Hui. PY - 2020/4/17. Y1 - 2020/4/17. N2 - Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level. A rapid method to enrich intact glycopeptides is essential for the analysis of glycoproteins, especially for biopharmaceutical proteins. In this study, we established a one-step method for the rapid capture of intact glycopeptides for analysis by mass spectrometry. Compared to the conventional sequential enrichment method, the one-step intact glycopeptide enrichment method reduced the sample preparation time and improved the detection of intact glycopeptides with long sequences or non-polar amino acids. Moreover, an increased ...
TY - JOUR. T1 - Molecular basis of antibody binding to mucin glycopeptides in lung cancer. AU - Qu, Jin. AU - Yu, Hongtao. AU - Li, Fenge. AU - Zhang, Chunlei. AU - Trad, Ahmad. AU - Brooks, Cory. AU - Zhang, Bin. AU - Gong, Ting. AU - Guo, Zhi. AU - Li, Yunsen. AU - Ragupathi, Govind. AU - Lou, Yanyan. AU - Hwu, Patrick. AU - Huang, Wei. AU - Zhou, Dapeng. PY - 2016/2. Y1 - 2016/2. N2 - Glycopeptides bearing Tn epitopes are emerging targets for cancer diagnosis and immunotherapy. In this study, we analyzed membrane proteins containing O-glycosylated tandem repeat (TR) sequences in lung cancer patients of different types and stages, using gene microarray data in public domain. The expression of Tn and glycopeptide epitopes on the surface of lung cancer cell lines were studied by monoclonal IgG antibodies 14A, 16A, and B72.3. The binding of mAbs to synthetic glycopeptides were studied by surface plasmon resonance. Nine mucin mRNAs were found to be expressed in lung cancer patients but at similar ...
TY - JOUR. T1 - Formation of homogeneous cross-linked lattices between oligomannose type glycopeptides and concanavalin A. AU - Islam Khan, M.. AU - Bhattacharyya, Lokesh. AU - Brewer, Curtis F.. PY - 1988/5/16. Y1 - 1988/5/16. N2 - Certain oligomannose type glycopeptides have previously been shown to be bivalent for binding to concanavalin A, and to give quantitative precipitation profiles with the protein that consist of single peaks which correspond to the binding stoichiometry of glycopeptide to protein monomer (1:2) (Bhattacharyya, L., Ceccarini, C., Lorenzoni, P., and Brewer, C.F. (1987) J. Biol. Chem. 262, 1288-1293). In the present study, equimolar mixtures of two oligomannose type glycopeptides, a Man-6 and a Man-9 glycopeptide, gives a quantitative precipitation profile which shows two protein peaks. Each glycopeptide was radiolabelled with 3H or 14C, and the the precipitation profiles of the individual glycopeptides in the mixture determined. The results show that the radioactivity ...
immune Uncategorized Mouse monoclonal to IGFBP2, Ziprasidone manufacture Copeptin, the C-terminal section of provasopressin, has emerged as a novel prognostic marker after hemorrhagic or ischemic stroke. the positive association of copeptin with poor prognosis after stroke was consistent in each stratified analysis. The present meta-analysis suggests that early measurement of plasma copeptin could provide better prognostic information about functional outcome and mortality in Ziprasidone manufacture Mouse monoclonal to IGFBP2 patients with acute stroke. Stroke is a disastrous disease; it is one of the leading causes of death and serious disability worldwide1. Mortality after 1 year ranges between 21% and 27%; approximately 15% to 30% of stroke survivors are permanently disabled1. It is necessary to evaluate prognostic factors to predict functional outcomes and mortality after stroke, which could be effective in creating therapeutic strategies and improving survival rates. Patient age and stroke ...
Four glycopeptides (I, IIA, IIB, III) with different oligosaccharide structures were isolated from purified mouse thymocyte Thy-1 glycoprotein. The glycoprotein was digested with Pronase, and the glycopeptide fraction was isolated by gel filtration and acetylated with [3H]acetic anhydride. The different glycan structures were separated by affinity chromatography on concanavalin A-Sepharose 4B and lentil lectin-Sepharose 4B. Size determinations of intact and exoglycosidase- and endoglycosidase-digested glycopeptides were performed by gel filtration on Bio-Gel P-6, calibrated with glycopeptides of known structure. On the basis of these experiments and on the behaviour of the glycopeptides on the lectin columns, the following structures of the oligosaccharide chains were proposed: I, triantennary complex-type with terminal fucose; IIA, biantennary complex-type without fucose; IIB, biantennary complex-type with fucose; III, a mixture of high-mannose chains containing either five or six ...
article{e00c4d89-ef12-4d42-9fc1-b94975575fa4, abstract = {,p,OBJECTIVE: Because elevated copeptin, a marker of vasopressin, is linked to low water intake and high diabetes risk, we tested the effect of water supplementation on copeptin and fasting glucose.,/p,,p,DESIGN, SETTING, AND PARTICIPANTS: Thirty-one healthy adults with high copeptin (>10.7 pmol · L-1 in men and >6.1 pmol·L-1 in women) identified in a population-based survey from 2013 to 2015 and with a current 24-hour urine osmolality of >600 mOsm · kg-1 were included.,/p,,p,INTERVENTION: Addition of 1.5 L water daily on top of habitual fluid intake for 6 weeks.,/p,,p,MAIN OUTCOME MEASURE: Pre- and postintervention fasting plasma copeptin concentrations.,/p,,p,RESULTS: Reported mean water intake increased from 0.43 to 1.35 L · d-1 (P < 0.001), with no other observed changes in diet. Median (interquartile range) urine osmolality was reduced from 879 (705, 996) to 384 (319, 502) mOsm · kg-1 (P < 0.001); urine volume ...
Correlation between plasma copeptin levels and other markers used in management of diabatic ketoacidosis in the first 36 hours after diagnosis- Plasma copeptin levels at diagnosis of diabate mellitus type 1 without ketoacidosis in ...
August 19th, 2016 - Creative Peptides, a professional supplier of peptides manufacturing upon academic, clinical, commercial and government laboratories in diverse applications, has released its efficient Glycopeptide Synthesis service, to help speed up the advance in solid phase methods. Nowadays, glycopeptides have played a pivotal role in a myriad of organisms and systems, such as biology, physiology, medicine, bioengineering and technology, etc. As is known, synthetic glycopeptides are able to offer an unique frontier for research in glycobiology and proteomics as well as for drug discovery & development, drug delivery & targeting, diagnostics development and biotechnological applications, which also promotes the development of modern biomarker discovery process. Based on rapid achievements in peptides research, increasing number of scientists are trying to discover more effective methods in modern scientific research, such as deslorelin acetate, aviptadil acetate, Chimeric Peptides, and so on.
Selective enrichment of glycopeptides from complicated biological samples is essential for MS-based glycoproteomics, but still remains a great challenge. In this study, we report an unprecedented ligand-free strategy for the selective enrichment of glycopeptides by simply utilizing the multivalent interactio
A number of recent advances in the chemical synthesis of glycopeptides and glycoproteins are described, with particular focus on the development of peptide ligation strategies and their implementation in the convergent assembly of complex glycopeptides. Recent applications in the synthesis of full length homogeneou RACI100: Celebrating Australian Chemistry
TY - JOUR. T1 - Edible Birds Nest. T2 - Physicochemical Properties, Production, and Application of Bioactive Extracts and Glycopeptides. AU - Daud, NurAliah. AU - Mohamad Yusop, Salma. AU - Babji, Abdul Salam. AU - Lim, Seng Joe. AU - Sarbini, Shahrul Razid. AU - Hui Yan, Tan. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Edible birds nest (EBN), the swiftlets nest, has been consumed for centuries as a tonic or health food. The information upon the usage and benefits of the EBN are largely based on historical and observational reports since its discovery, until some were proven scientifically at recent times. Studies have been conducted on the major components of the EBN, i.e. glycoprotein, in attempts to elucidate the roles of the glycoprotein in terms of functionality toward health improvement. This article presents a review of the physicochemical and nutritional composition of EBN from different harvested locations and its functional properties. The conversion of EBN glycoproteins to glycopeptides ...
TY - JOUR. T1 - Identification of O-glycosylated decapeptides within the MUC1 repeat domain as potential MHC class I (A2) binding epitopes. AU - Ninkovic, Tanja. AU - Kinarsky, Leo. AU - Engelmann, Katja. AU - Pisarev, Vladimir. AU - Sherman, Simon. AU - Finn, Olivera J.. AU - Hanisch, Franz Georg. N1 - Funding Information: The work was supported by NIH grant 1RO1 CA84106 and the Köln-Fortune Programme (to F.G.H.) and by NIH grant 2PO CA73743 (to O.J.F.).. PY - 2009/11. Y1 - 2009/11. N2 - The MUC1 glycoprotein is considered a tumor antigen due to its over expression and aberrant glycosylation in cancer tissues. The latter results in appearance of new antigenic tumor specific glycopeptides not found on normal glycoforms of the mucin. MUC1 glycopeptides can be presented by APCs on MHC class II molecules to activate glycopeptide specific helper T-cells. No study has yet reported presentation of MUC1 glycopeptides on MHC class I molecules as stimulators of cytotoxic T-cells. In this study we show ...
RESULTS: Multivariable linear regression analysis revealed stressful delivery (regression coefficient β = .569, P ,0.001), acidosis (β = -.347, P , .001), and male gender (β = .132, P , .01) as independent determinants of copeptin at birth. In infants born without stress, that is by primary cesarean section (n = 81), male gender was the sole variable associated with copeptin (β = .286, P , .05), copeptin concentrations being higher in boys [median 5.5 pmol/L (interquartile range 4.4-10.2)] than in girls [4.8 pmol/L (interquartile range 3.6-5.8), P , .05]. At day 3 of life, copeptin was determined independently by postnatal physiological dehydration (β = .485; P , .001) and birth weight (β = .279; P , .01 ...
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The goal of the proposed research is to develop a general and highly efficient chemo enzymatic method for making diverse homogeneous glycopeptides and glycoprot...
We report here the use of TEV protease cleavable fusion proteins to produce glycosylated bioactive peptides and proteins. Bacterial expression was utilized to produce two fusion proteins, GPRT-C37-H6 and His-tagged interleukin-2 (amino acids 6-133), which when cleaved by the tobacco etch virus NIa protease (TEV protease) to generate HIV entry inhibitor peptide C37-H6 and a truncated version of the cytokine interleukin-2, both containing N-terminal cysteines. The N-terminal cysteine containing C37-H6 and truncated interleukin-2 were then joined to a synthetic glycopeptide thioester utilizing native chemical ligation under nondenaturing and denaturing conditions, respectively. The ligations of the glycopeptide to the C37-H6 peptide and the truncated interleukin-2 protein both proceeded in high yield, though the size, and physical properties of the two polypeptides differ greatly ...
Synthesis of glycopeptides containing carbohydrate and peptide recognition motifs. Novel enzymatic mechanisms in carbohydrate metabolism
Antimicrobial glycopeptides of innate immune origin : The resistance of insects to bacterial infection is explained by their ability to synthesize a diverse range of antibacterial peptides. A remarkable feature of some proline rich antibacterial peptides such as drosocin, pyrrhocoricin, formaecins, diptericin and lebocins which have been isolated from insects, is the presence of conserved O-glycosylated threonine residue and the integrity of the carbohydrate side chain is necessary for their maximum activity. The exact role of the sugar remains an enigma. We are addressing the problem of role of carbohydrate domains in the activity of antimicrobial glycopeptides by studying their structure-function relationship. However, the mode of action of this class of cationic antimicrobial glycopeptides is not known completely and they are thought to kill bacteria by entering cells and binding to target/targets. The studies addressing mechanistic aspects are also undertaken and are in progress for the ...
To control for potential effects on mortality because of reverse causation, we did a sensitivity analysis excluding patients with major stroke, but results remained qualitatively unchanged (Table 2). In addition, analysis of copeptin by tertiles or by using the 90th percentile of copeptin levels in controls (8.11 pmol/L) in patients revealed similar results (all vascular events: top tertile versus lowest tertile: adjusted HR, 1.91; 95% CI, 1.43-2.53; P,0.0001 and 90th percentile of controls in patients: adjusted HR, 1.76; 1.41-2.20; P,0.0001; Table I in the online-only Data Supplement). Use of the 95th percentile of controls (10.67 pmol/L) in patients revealed practically identical results (all vascular events: adjusted HR, 1.76; 1.40-2.21). The absolute 5-year risk for recurrent vascular events was 0.38 (95% CI, 0.33-0.42) for patients with copeptin levels ≥90th percentile in controls and 0.21 (0.18-0.25) for patients with levels below (Table II in the online-only Data Supplement).. Levels of ...
This page is Related Information of Analysis of Glycopeptides of Monoclonal Antibody Using MALDI-7090 High-Resolution MALDI-TOF MS.
Glycopeptides and Lipoglycopeptides - Learn about the causes, symptoms, diagnosis & treatment from the MSD Manuals - Medical Consumer Version.
Fig. 3. Isolation and characterization of N332-dependent antibodies isolated using native-like SOSIP trimers and synthetic Man9-V3 glycopeptide.. (A) ELISA binding analyses of plasma from the HIV-infected donor CH765 to Man9-V3 and aglycone V3 peptides. Donor plasma was screened in duplicate assays, and binding is represented as mean values. (B) Memory B cells from donor CH765 were decorated with either fluorophore-conjugated BG505.T332N.SOSIP and DU156.12.SOSIP in phycoerythrin (PE) and allophycocyanin (APC), or Man9-V3 tetramers tagged to SA-AF647 (Alexa Fluor 647) and SA-BV421 (Brilliant Violet 421). The B cells from which CH765-VRC41.01, VRC41.02, and DH563 were cloned are indicated as green, red, and blue dots, respectively. (C) Immunogenetics and phylogeny of the DH563-VRC41 clonal lineage were inferred using Clonanalyst (33, 34). See also fig. S3. nt, nucleotide. (D) Reactivity of CH765-VRC41.01, CH765-VRC41.02, and DH563 to Man5, Man6, Man7-D1, Man7-D3, Man8-D1D3, and Man9 glycans, ...
A mass spectrometry-based glycoproteomic characterization of zebrafish embryos was performed to identify the N-linked glycoproteins and N-linked glycosylation sites. To increase the number of glycopeptides, proteins from zebrafish were digested with two different proteases--chymotrypsin and trypsin--into peptides of different length. The N-glycosylated peptides of zebrafish were then captured by the solid-phase extraction of N-linked glycopeptides (SPEG) method and the peptides were identified with an LTQ OrbiTrap Velos mass spectrometer.
Waters Glycoprotein BEH Amide 300A, 1.7um columns were primarily designed for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides.. Each batch of Glycoprotein BEH Amide 300A 1.7um material is specifically quality control tested with the Waters Glycoprotein Performance Test Standard (P/N 86008010 that includes a mixture of Ribonuclease A as well as Ribonulease B glycoforms) to help ensure batch to batch consistency. This kit includes one Glycoprotein BEH Amide 300A, 1.7um column and one vial of Waters Glycoprotein Performance Test Standard. The standard is included to be able to immediately benchmark the column upon receipt and can be reordered as a separate part for continued monitoring. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis.
Waters Glycoprotein BEH Amide 300A, 1.7um columns were primarily designed for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides.. Each batch of Glycoprotein BEH Amide 300A 1.7um material is specifically quality control tested with the Waters Glycoprotein Performance Test Standard (P/N 86008010 that includes a mixture of Ribonuclease A as well as Ribonulease B glycoforms) to help ensure batch to batch consistency. This kit includes one Glycoprotein BEH Amide 300A, 1.7um column and one vial of Waters Glycoprotein Performance Test Standard. The standard is included to be able to immediately benchmark the column upon receipt and can be reordered as a separate part for continued monitoring. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis.
Chest pain is a clinical problem which often results in emergency department visits and is characterized by difficult differential diagnosis. Although..
Only 1 in 10 patients with acute chest pain in the emergency department turn out to have an acute myocardial infarction, yet many are not released from the hospital until after 6 to 12 hours of cardiac monitoring and multiple ECG and troponin tests. The search for a test that can rule out MI early in the process has proved elusive.. The Biomarkers in Cardiology-8 (BIC-8) trial, presented at the European Society of Cardiology meeting in Amsterdam, was designed to determine the utility of the combination of troponin and copeptin testing. Copeptin is a marker of severe hemodynamic stress. After an acute MI, copeptin levels increase rapidly. In earlier observational studies, the combination test was found to have a negative predictive value of 99%.. Some 902 patients with suspected ACS who were troponin-negative were randomized to standard treatment or an experimental strategy in which those with a negative copeptin test were discharged early. At 30 days, there were no significant differences in the ...
This method describes the conjugation of a synthetic glycopeptide to the N-terminus of a recombinant human interleukin-2 (IL-2) protein fragment. The IL-2 protein fragment is produced as an...
Nilsson J, Rüetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G. Enrichment of glycopeptides for glycan structure and attachment site identification ...
Bubendorf/Basel, October 7, 2014 - Bachem the leading peptide manufacturer, and GlyTech Inc. (Kyoto, Japan), the world-leading developer of glycosylated drugs, announced today that they have entered into a co-promotion agreement for GlyTechs glycosylation technology. As part of the agreement, Bachem will leverage its sales and marketing resources to enhance GlyTechs partnering efforts and GlyTech will promote Bachem as the partner of choice for manufacture of its glycopeptides and glycoproteins. Press release. ...
Cancers Vaccine - Synthetically generated glycopeptides from tumor cells may stimulate the formation of specific antibodies Tumor cells and healthy somatic cells differ in the structures on their surfaces. Because these distinctions between surface area antigens are minimal frequently, all initiatives in this direction have far failed thus; it is not feasible to elicit a targeted immune response directed specifically against the tumor cells. A united group of researchers headed by Horst Kunz in Mainz, Germany, has now come a step further. They have successfully developed a artificial vaccine that induces a solid and particular immune response against the desired tumor antigen in mice.. ...
The Medicines Company (NASDAQ: MDCO) today announced that the U.S. Food and Drug Administration (FDA) has designated oritavancin as a Qualified Infectious Disease Product (QIDP)
Dr. Blanc is a postdoc supervised by Fraden. Blancs role on this IRG1 project was calculation of the liquid crystalline phase behavior. This work was partially supported by the MRSEC). Zhou, J., Du, X., Chen, X. and Xu, B., Adaptive Multifunctional Supramolecular Assemblies of Glycopeptides Rapidly Enable Morphogenesis, Biochemistry 57, 4867-4879 (2018) doi: 10.1021/acs.biochem.8b00125.. Feng, Z., Wang, H., Wang, S., Zhang, Q., Zhang, X., Rodal, A. A. and Xu, B., Enzymatic Assemblies Disrupt the Membrane and Target Endoplasmic Reticulum for Selective Cancer Cell Death, J Am Chem Soc 140, 9566-9573 (2018) doi: 10.1021/jacs.8b04641.. Lazaro, G. R., Dragnea, B. and Hagan, M. F., Self-assembly of convex particles on spherocylindrical surfaces, Soft Matter 14, 5728-5740 (2018) doi: 10.1039/c8sm00129d.. Zeng, C, Rodriguez Lazaro, G, Tsvetkova, IB, Hagan, MF, & Dragnea, B. Defects and Chirality in the Nanoparticle-Directed Assembly of Spherocylindrical Shells of Virus Coat Proteins. ACS Nano, ...
Video articles in JoVE about galectin 1 include Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow, Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells, Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve, Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry.
TY - JOUR. T1 - Antibacterial activity of the new glycopeptide antibiotic SKF104662. AU - Jorgensen, J. H.. AU - Redding, J. S.. AU - Maher, L. A.. PY - 1989/1/1. Y1 - 1989/1/1. N2 - The inhibitory activity of the new glycopeptide antibiotic SKF104662 was generally equivalent (±1 concentration increment) to the activities of vancomycin, teicoplanin, and daptomycin against selected gram-positive bacteria. However, SKF104662 demonstrated greater activity against Staphylococcus epidermidis and S. haemolyticus than did teicoplanin and was more active than the other drugs against Clostridium difficile isolates. SKF104662 possessed bactericidal activity quite similar to that of vancomycin against selected isolates of Staphylococcus, Streptococcus, and Enterococcus species.. AB - The inhibitory activity of the new glycopeptide antibiotic SKF104662 was generally equivalent (±1 concentration increment) to the activities of vancomycin, teicoplanin, and daptomycin against selected gram-positive bacteria. ...
Aim: To explore the association of plasma copeptin, the C-terminal portion of provasopressin and a stable surrogate marker for arginine vasopressin secretion, with plasma glucagon in obese men and men of normal weight. Methods: We measured fasting blood concentrations of copeptin and glucagon in 102 healthy obese men (mean ± sd age 49.4 ± 10.2 years) and a control group 27 healthy men of normal weight (mean ± sd age 51.5 ± 8.4 years). Differences between groups were evaluated using t-tests, and multiple linear regression analysis, adjusting for age and weight status (normal weight vs obese), was used to calculate unstandardized regression coefficients (β) with 95% CIs between copeptin and glucagon. Copeptin was (natural) log-transformed. Results: The obese men had higher [median (interquartile range)] plasma copeptin concentrations [6.6 (4.6-9.5) vs 4.9 (3.5-6.8) pmol/l; P = 0.040] and higher mean ± sd plasma glucagon concentrations (8.5 ± 3.8 vs 5.3 ± 1.4 pmol/l; P , 0.001) than the ...
Background. Copeptin has been known to predict heart failure and cardiovascular death in patients with acute coronary syndrome.. Methods. We collected coronary arterial blood samples from the infarct artery during primary percutaneous coronary intervention (PCI) in 80 STEMI pts and 28 controls. We assessed commonly used cardiac biomarkers (CK, CK-MB, troponin-I, CRP) and additionally measured recently introduced biomarkers (Copeptin [C-terminal Provasopressin] and N-terminal pro-B-type natriuretic peptide [NT-proBNP]).. Results. Pt age was 58±12 yrs and 93% were males. Intravascular ultrasound (IVUS) of the culprit lesion in 67 pts showed ruptured plaques in 28 pts. STEMI pts had a higher Copeptin level (265.89±183.11 pmol/L) vs193.67±60.16 pmol/L in normal controls, p=0.005. Especially, Copeptin levels were higher in ruptured plaque compared to non-ruptured plaques: 318.83±209.34 pmol/L vs 221.99±151.49 pmol/L, p=0.034. While troponin I, CK-MB, and CRP were not correlated with Copeptin, ...
Regardless of cardiac surgical procedure and perioperative course, all patients had an early significant rise of copeptin concentrations, generally peaking at weaning from CBP or upon arrival in the intensive care unit. Among patients with normal copeptin concentration preoperatively and uneventful …
The median concentration of copeptin at admission was lower in AECOPD compared to acute HF (8.8 [5.2-19.7] vs. 22.2 [10.2-47.9]) pmol/L, p < 0.001), but NT-proBNP discriminated acute HF from non-HF related dyspnea more accurately than copeptin (ROC-AUC 0.85 [0.81-0.89] vs. 0.71 [0.66-0.77], p < 0.0001). Adjusted for basic risk factors, increased copeptin concentrations predicted mortality in AECOPD (HR per log (ln) unit 1.72 [95% CI 1.21-2.45], p = 0.003) and acute HF (1.61 [1.25-2.09], p < 0.001), whereas NT-proBNP concentrations predicted mortality only in acute HF (1.62 [1.27-2.06], p < 0.001). On top of a basic model copeptin reclassified a significant proportion of patients into a more accurate risk strata in AECOPD (NRI 0.60 [0.19-1.02], p = 0.004) and acute HF (0.39 [0.06-0.71], p = 0.020). CONCLUSION ...
Crude extracts of Vicia graminea seeds contain an agglutinin that is blood group N specific. The lectin exhibits a complex carbohydrate specificity. It is not inhibited by any mono- or disaccharides, although the O-linked blood group N antigen is a disaccharide with the structure Gal β(1,3)GalNAc. This carbohydrate structure is also recognized by the peanut lectin, but PNA binds to other galactose containing glycoconjugates as well. The peptide portion of the blood group N antigen is critical for binding of VGA. The extended binding site of the lectin reacts with clusters of O-linked Gal β(1,3)GalNAc adjacent to an N-terminal leucine residue 1,2 . Disaccharide clusters located on the first eight amino acids of O-linked glycopeptides react more strongly than these same clusters located on other amino acid residues 2 . VGA will bind to glycoproteins containing sialic acid, but removal of terminal sialic acid residues from these glycoproteins increases the reactivity of VGA. Both VGA and PNA can ...
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.. The study of protein glycosylation has lagged far behind the progress of current proteomics because of the enormous complexity, wide dynamic range distribution and low stoichiometric modification of glycoprotein. Solid phase extraction of tryptic N-glycopeptides by hydrazide chemistry is becoming a popular protocol for the analysis of N-glycoproteome. However, in silico digestion of proteins in human proteome database by trypsin indicates that a significant percentage of tryptic N-glycopeptides is not in the preferred detection mass range of shotgun proteomics approach, that is, from 800 to 3500 Da. And the quite big size of glycan groups may block trypsin to access the K, R residues near N-glycosites for digestion, which will result in generation of big glycopeptides. Thus many N-glycosites could not be localized if only trypsin was used to digest proteins. Herein, we describe a comprehensive way to analyze the N-glycoproteome ...
Kathrin Stavenhagen: Glycopeptide analysis remains challenging because of its sample heterogeneity resulting from the degree of glycosylation site occupancy (macroheterogeneity) and the different glycoforms attached to individual glycosylation sites (microheterogeneity).. With respect to the latter one, qualitative site-specific glycosylation information of glycoproteins can be obtained by unspecific protease treatment resulting in small amino acid stretches carrying the glycan. This improves determination of the glycosylation sites. However, detecting these glycopeptides by 1D-LC-ESI-MS/MS is challenging due to insufficient or irreversible retention on the stationary phase and thus multiple analyses with different LC-setups are required. Since biological sample amounts are usually limited, methods for acquiring comprehensive information in a single run are necessary.. To obtain qualitative information of the glycosylation site we set up an integrated C18-porous graphitized carbon ...
Although it typically evades the immune system, HIV does have sites of vulnerability that can be targeted in vaccine design. One such site is a glycan near the V3 loop of the envelope protein, but antibodies recognizing this epitope are often not detected in people infected with HIV. Alam et al. designed a synthetic glycopeptide that can identify B cells targeting this epitope and also used it to immunize macaques. Bonsignori et al. used this synthetic glycopeptide and other baits to study the V3-glycan antibody responses of an HIV-infected individual that developed broadly neutralizing antibodies. They also examined viral evolution over time and found clues as to why these types of antibodies do not develop more often. These tools and findings could pave the way for a vaccine that protects against diverse strains of HIV. ...
Kailemia MJ, Wei W, Nguyen K, Beals E, Sawrey-Kubicek L, Rhodes C, Zhu C, Sacchi R, Zivkovic AM, Lebrilla CB. Targeted Measurements of O- and N-Glycopeptides Show That Proteins in High Density Lipoprotein Particles Are Enriched with Specific Glycosylation Compared to Plasma. J Proteome Res. 2018 02 02; 17(2):834-845 ...
1) Kaji H, Saito H, Yamauchi Y, Shinkawa T, Taoka M, Hirabayashi J, Kasai K, Takahashi N, Isobe T. Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins. Nat Biotechnol. 2003 Jun;21(6):667-72. [PMID: 12754521]. 2) Kaji H, Yamauchi Y, Takahashi N, Isobe T. Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging. Nat Protoc. 2006;1(6):3019-27. [PMID: 17406563]. 3) Shinkawa T, Taoka M, Yamauchi Y, Ichimura T, Kaji H, Takahashi N, Isobe T. STEM: a software tool for large-scale proteomic data analyses. J Proteome Res. 2005 Sep-Oct;4(5):1826-31. [PMID: 16212438]. 4) Kaji H, Kamiie J, Kawakami H, Kido K, Yamauchi Y, Shinkawa T, Taoka M, Takahashi N, Isobe T. Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins. Mol Cell Proteomics. 2007 Dec;6(12):2100-9. ...
AMI was the final diagnosis in 156 patients (7.9%). A negative copeptin and cTnI at baseline ruled out AMI for 58% of patients, with a negative predictive value (NPV) of 99.2%. AMIs not detected by the initial cTnI alone were picked up with copeptin >14 pmol/L in 23/32 patients (72%). Non−ST-segment elevation MIs (NSTEMIs) undetected by cTnI at 0 hours were detected with copeptin >14 pmol/L in 10/19 patients (53%). Projected average time-to-decision could be reduced by 43% (from 3.0 hours to 1.8 hours) by the early rule out of 58% of patients. Both abnormal copeptin and cTnI were predictors of death at 180 days (p < 0.0001 for both, c-index 0.784 and 0.800, respectively). Both were independent of age and each other and provided additional predictive value (p < 0.0001).. ...
Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MS n Shiaw-Lin Wu Pavel Bondarenko Tom Shaler Paul Shieh and William S. Hancock Protein Glycosylation The data presented here can be acquired using a,Structural,Analysis,of,Glycosylated,Peptides,in,Complex,Mixtures,with,,,,,,,,,,,,Ion,Trap,MSn,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
There is a very strong correlation between the affinity of peptide binding to MHC molecules and the capacity of the peptide to induce an immune response (31, 32). This information is critical to the strategy used in this study. The size of a peptide determines its capacity to bind class I MHC. Whereas the optimal peptide length for most murine MHC molecules appears to be 9-amino acid residues, the Kb and Kk molecules prefer 8-residue peptides (33). Kb molecules were initially chosen to define a glycopeptide that: (a) binds with high affinity; and (b) will be of a structure that skews the T cell response toward recognition of the carbohydrate moiety rather than toward either the peptide or the MHC molecule. The critical anchor residues for Kb binding have been canonically defined as phenylalanine (F) or tyrosine (Y) in position 5 and leucine (L) or methionine (M) at position 8 of an octamer peptide (8). The maintenance of critical hydrogen bonding between main chain atoms in the peptide and atoms ...
Perform Glycan and Glycopeptide Qualitative Analysis, Glycan Structure Prediction & Multi stage Mass Spectrometry (MSn) data analysis using accurate ranking mechanism.
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Seven in one blow: The efficient formation of mixed disulfides on the thiol-rich fusion protein A followed by subsequent intein cleavage gave the fragment B with all seven cysteines protected against oxidation. The native chemical ligation of B with synthetic glycopeptide thioesters provides glycoproteins ...
FDA Accepts Filing of NDA for IV Antibiotic Oritavancin with Priority Review Oritavancin ( INN, also known as LY333328) is a novel semi-synthetic glycopeptide antibiotic being developed for the treatment of serious Gram-positive infections. Originally discovered and developed by Eli Lilly, oritavancin was acquired by InterMune in 2001 and then by Targanta Therapeutics in late 2005. In Dec 2008 the FDA declined to approve […]. ...
FDA Accepts Filing of NDA for IV Antibiotic Oritavancin with Priority Review Oritavancin ( INN, also known as LY333328) is a novel semi-synthetic glycopeptide antibiotic being developed for the treatment of serious Gram-positive infections. Originally discovered and developed by Eli Lilly, oritavancin was acquired by InterMune in 2001 and then by Targanta Therapeutics in late 2005. In Dec 2008 the FDA declined to approve […]. ...
1GAC: Conformation of A82846B, a Glycopeptide Antibiotic, Complexed with its Cell Wall Fragment: An Asymmetric Homodimer Determined Using NMR Spectroscopy.
An estimate of the relative abundance of the various O-glycans was determined from nanoLC-ESI-IT-MS analysis of trypsin-generated (glyco)peptides. It should be noted that because the signal of the monosialylated O-glycopeptide in triply charged state overlapped with the doubly charged peak of the hinge repeat peptide with a putative acetylation modification in all samples, only the doubly charged signal of the monosialylated O-glycopeptide was quantified, thereby leading to an underestimation of this O-glycoform. Furthermore, it is known that glycopeptides with different glycan structures can have different response factors (35), and thus the relative abundances we measured may not accurately reflect the real ratios. In order to obtain a more reliable estimate of the percentage of the hinge repeat motif bearing an O-glycan, tryptic IgG peptides were incubated with exoglycosidases, trimming all O-glycans down to a single N-acetylhexosamine. A previous study of quantitative measurements of a ...
Danishefsky, S. J.; Hu, S.; Cirillo, P. F.; Eckhardt, M.; Seeberger, P. H.: A highly convergent total synthetic route to glycopeptides carrying a high-mannose core pentasaccharide domain N-linked to a natural peptide motif. Chemistry - A European Journal 3 (10), pp. 1617 - 1628 (1997 ...
Despite a known biological importance, the glycoconjugate complexity has been an obstacle to their systematic analysis. Bruker is now delivering the tools to successfully address the challenges of glyco analysis. This includes glycopeptides, glycans, and glycoproteins and thus supports all common strategies.. Glyco Analysis ...
Stavenhagen, K.; Hinneburg, H.; Thaysen-Andersen, M.; Hartmann, L.; Varón Silva, D.; Fuchser, J.; Kaspar, S.; Rapp, E.; Seeberger, P. H.; Kolarich, D.: Quantitative mapping of glycoprotein micro-heterogeneity and macro-heterogeneity: an evaluation of mass spectrometry signal strengths using synthetic peptides and glycopeptides. Journal of Mass Spectrometry 48 (6), pp. 627 - 639 (2013 ...
Teicoplanin Teicoplanin Systematic (IUPAC) name  ? Identifiers CAS number 61036-62-2 ATC code J01XA02 PubChem  ? Chemical data Formula Variable Mol.
The ovary produces (1) gametes-germ cells in the ovary have undergone the first meiotic division to become oocytes in primordial follicles by the time of birth, with about 400 of these ovulating during reproductive life; and (2) hormones-oestradiol, progesterone, androgens, and two nonsteroidal glycopeptides, inhibin A and B....
Copeptin predicts prognosis in patients with heart failure, according to research presented at the ESC Congress today, August 25, by Professor Stefan Störk from Germany.
192126-76-4 - MQBCDKMPXVYCGO-FQBKTPCVSA-N - Mycothiol - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Tammy Lau - The Importance of Protocol Design for RNA-Seq in the Ongoing Development of a Novel Technology for Tissue Specific Gene Expression Profiling in C. elegans. Arjun Sharma - The Glycopeptide Resistance Predictor. ...
by Ramakrishnan (1994). Glycopeptide antibiotics. New York u.a.: Dekker. ISBN 0-8247-9193-2.CS1 maint: extra text: authors list ...
It is a type of glycopeptide antibiotic and works by blocking the construction of a cell wall. Vancomycin was first sold in ... These P450s are recruited by the X domain present in the 7th NRPS module, which is unqiue to glycopeptide antibiotic ... Glycopeptide-Intermediate Staphylococcus aureus Working Group". The New England Journal of Medicine. 340 (7): 493-501. doi: ... Pootoolal J, Neu J, Wright GD (2002). "Glycopeptide antibiotic resistance". Annual Review of Pharmacology and Toxicology. 42: ...
O-linked glycopeptides recently have been shown to exhibit excellent CNS permeability and efficacy in multiple animal models ... The peculiar nature of O-linked glycopeptides is that there are numerous examples which are CNS penetrant. The fundamental ... "Biousian glycopeptides penetrate the blood-brain barrier". Tetrahedron: Asymmetry. Carbohydrate Science. Part 1. 16 (1): 65-75 ...
... glycopeptides, bacitracin, and fosfomycin.". In Giguère S, Prescott JF, Dowling PM (eds.). Antimicrobial Therapy in Veterinary ...
Bruch RC, White HB (October 1982). "Compositional and structural heterogeneity of avidin glycopeptides". Biochemistry. 21 (21 ...
Thirty-five of these ORFs are similar to those found in other glycopeptide gene clusters. The function of each of these genes ... It is a semisynthetic glycopeptide antibiotic with a spectrum of activity similar to vancomycin. Its mechanism of action is to ... Heydorn A, Petersen BO, Duus JO, Bergmann S, Suhr-Jessen T, Nielsen J (March 2000). "Biosynthetic studies of the glycopeptide ... Peschke M, Brieke C, Cryle MJ (October 2016). "F-O-G Ring Formation in Glycopeptide Antibiotic Biosynthesis is Catalysed by ...
... s are a class of antibiotic that have lipophilic side-chains linked to glycopeptides. The class includes ... Van Bambeke F. (August 2006). "Glycopeptides and glycodepsipeptides in clinical development: a comparative review of their ... Telavancin#Mechanism of action Kahne, Dan; Leimkuhler, Catherine; Lu, Wei; Walsh, Christopher (2005). "Glycopeptide and ...
The systematic name of this enzyme class is N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase. ... Plummer TH, Tarentino AL (Oct 1981). "Facile cleavage of complex oligosaccharides from glycopeptides by almond emulsin peptide ... Takahashi N (Jun 1977). "Demonstration of a new amidase acting on glycopeptides". Biochemical and Biophysical Research ... Other names in common use include: glycopeptide N-glycosidase, glycopeptidase, N-oligosaccharide glycopeptidase, N-glycanase, ...
"Nomenclature of glycoproteins, glycopeptides and peptidoglycans, Recommendations 1985". www.qmul.ac.uk. Retrieved 16 March 2021 ...
"Nomenclature of glycoproteins, glycopeptides and peptidoglycans, Recommendations 1985". www.qmul.ac.uk. Retrieved 16 March 2021 ... and structural analysis of glycoproteins are Ero1 Female sperm storage Glycocalyx Glycome Glycopeptide Gp120 Gp41 Miraculin P- ... a new post-translational modification found in glycopeptide bacteriocins". FEBS Letters. 585 (4): 645-650. doi:10.1016/j. ...
In year 2006, LaPlante focused her study on a glycopeptide antibiotic "Oritavancin", its development, usage and efficacy in ... Ward, Kristina E.; Mersfelder, Tracey L.; LaPlante, Kerry L. (April 1, 2006). "Oritavancin - an investigational glycopeptide ...
... is uniformly susceptible to glycopeptides and oxazolidones. Diernhofer first used the name ...
... glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides.[citation needed] Much of the chemistry of glycosides is ...
Walsh I.; Zhao S.; Campbell M.; Taron C.H.; Rudd P.M. (2016). "Quantitative profiling of glycans and glycopeptides: an ...
DHGP is also found in vancomycin and related glycopeptides. Although the (S) stereoisomer is synthesized by the DpgA-D enzymes ... Chen, H., Tseng, C. C., Hubbard, B. K., Walsh, C. T. "Glycopeptide antibiotic biosyntehsis: Enzymatic assembly of the dedicated ... Yim, G., Thaker, M. N., Koteva, K., Wright, G. "Glycopeptide antibiotic biosynthesis." The Journal of Antibiotics, 2017, 67, 31 ... "A Polyketide Synthase in glycopeptide Biosynthesis: the Biosynthesis of the Non-Proteogenic Amino Acid (S)-3,5- ...
... is a macrolide glycopeptide antibiotic. Dirithromycin (Dynabac) is a more lipid-soluble prodrug derivative of 9S- ...
This Antarctic marine fish is one of several in the region that produce antifreeze glycopeptides as an adaptation against the ... A. P. Wohrmann (1995). "Antifreeze glycopeptides in the high-Antarctic Silverfish Pleurogramma antarcticum (Notothenioidei)". ...
June 2020). "Parallel Glyco-SPOT Synthesis of Glycopeptide Libraries". Cell Chemical Biology. 27 (9): 1207-1219.e9. doi:10.1016 ...
Perichon B, Reynolds P, Courvalin P (September 1997). "VanD-type glycopeptide-resistant Enterococcus faecium BM4339". ...
Using a bait glycopeptide based roughly on a consensus sequence for O-GlcNAc, α-enolase, EBP1, and 14-3-3 were identified as ... Teo, CF; Ingale, S; Wolfert, MA; Elsayed, GA; Nöt, LG; Chatham, JC; Wells, L; Boons, GJ (May 2010). "Glycopeptide-specific ... Directed tandem MS and targeted glycopeptide assignment allow for identification of O-GlcNAcylated peptide sequences. One ... recoded glycopeptides from beads using mild acid Obtain a full-scan mass spectrum from isotopically recoded glycopeptides Apply ...
Sublancin may be an S-linked glycopeptide. They are synthesised with a leader polypeptide sequence that is removed only during ... "Sublancin is not a lantibiotic but an S-linked glycopeptide". Nat. Chem. Biol. 7 (2): 78-80. doi:10.1038/nchembio.509. PMC ...
Pigrau C, Almirante B (April 2009). "[Oxazolidinones, glycopeptides and cyclic lipopeptides]" [Oxazolidinones, glycopeptides ... A large meta-analysis of randomized controlled trials found linezolid to be more effective than glycopeptide antibiotics (such ... Falagas ME, Siempos II, Vardakas KZ (January 2008). "Linezolid versus glycopeptide or beta-lactam for treatment of Gram- ... Linezolid's spectrum of activity against Gram-positive bacteria is similar to that of the glycopeptide antibiotic vancomycin, ...
... new glycopeptide antibiotics produced by Actinoplanes missouriensis. The isolation and preliminary chemical characterization of ... new glycopeptide antibiotics produced by Actinoplanes missouriensis. The isolation and preliminary chemical characterization of ...
Glycopeptide resistance is mediated by acquisition of the vanA gene, which originates from the Tn1546 transposon found in a ... Also, glycopeptide antibiotics do not penetrate very well into infected tissues (this is a particular concern with infections ... Vancomycin-resistant S. aureus (VRSA) is a strain of S. aureus that has become resistant to the glycopeptides. The first case ... For this reason, vancomycin, a glycopeptide antibiotic, is commonly used to combat MRSA. Vancomycin inhibits the synthesis of ...
A glycopeptide containing two sites of oligosaccharide attachment". Biochimica et Biophysica Acta (BBA) - Protein Structure. ...
"Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides". Proteomics. 8 (14): 2858-2871. ...
These PBPs crosslink glycopeptides (D-alanyl-alanine), forming the peptidoglycan cell wall. Methicillin and other β-lactam ...
MOGS also acts to lesser extent on the corresponding glycolipids and glycopeptides. Elting JJ, Chen WW, Lennarz WJ (March 1980 ...
Brik, Ashraf; Ficht, Simon; Yang, Yu-Ying; Wong, Chi-Huey (2006-11-01). "Sugar-Assisted Ligation of N-Linked Glycopeptides with ... Payne, Richard J.; Wong, Chi-Huey (2010-01-07). "Advances in chemical ligation strategies for the synthesis of glycopeptides ... Preparation of Ribonuclease Glycoforms via Enzymatic Glycopeptide Condensation and Glycosylation". Journal of the American ...
"Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides". Proteomics. 8 (14): 2858-2871. ...
The glycopeptides are produced by spotting FMOC protected amino acids allowing the synthesis to be performed at microgram ( ... Glycopeptides are peptides that contain carbohydrate moieties (glycans) covalently attached to the side chains of the amino ... The synthesis of glycopeptides provides biological probes for researchers to elucidate glycan function in nature and products ... In this method, libraries of glycopeptides are produced on a cellulose surface (e.g. filter paper) which acts as the solid ...
Mannopeptimycin glycopeptide is a lead antibiotic peptide. Sum, Phaik-Eng; How, David; Torres, Nancy; Petersen, Peter J.; Lenoy ... "Novel ether derivatives of mannopeptimycin glycopeptide antibiotic". Bioorganic & Medicinal Chemistry Letters. 13 (6): 1151- ...
Marker ion detection during LC-ESMS facilitated collection of glycopeptide-containing fractions. Analysis of the glycopeptides ... Selective detection of glycopeptides on ion trap mass spectrometers.. Sullivan B1, Addona TA, Carr SA. ... Using these optimized conditions, marker ion detection and glycopeptide analysis could be achieved with as little as 10 pmol of ... of digested glycoproteins has been demonstrated to be a highly selective and sensitive approach for detection of glycopeptides ...
... Rapid Commun ... In this work, MRM was adopted for quantification of glycopeptides derived from both model glycoproteins and depleted human ... This approach can be applied to comparative and quantitative studies of glycopeptides in biological samples as illustrated for ... The results indicate that oxonium ions could be adopted for the characterization and quantification of glycopeptides in general ...
... hence glycopeptides are selectively toxic. Glycopeptides interact with peptides of this conformation by hydrogen bonding, ... As a result of binding to L-aa-D-Ala-D-Ala groups in wall intermediates, glycopeptides inhibit, apparently by steric hindrance ... Glycopeptide antibiotics, including vancomycin and teicoplanin, are large, rigid molecules that inhibit a late stage in ... Structure, biochemistry and mechanism of action of glycopeptide antibiotics.. Reynolds PE1. ...
We describe the various types of glycopeptide hypersensitivity reactions associated with glycopeptides and lipoglycopeptides, ... Parenteral glycopeptides are first-line therapy for severe MRSA infections; however, oral vancomycin is used as a first-line ... Although hypersensitivity to glycopeptides is rare, both immune-mediated and delayed reactions have been reported in the ... Common adverse effects associated with glycopeptide use include nephrotoxicity, ototoxicity, and Redman Syndrome (RMS). The RMS ...
White Paper: Orbitrap Fusion MS for Glycan and Glycopeptide Analysis. *Poster Note: Comparison and Optimization of First and ... Application Note: HILIC - An Alternative Separation Technique for Glycopeptides. *Brochure: Glycan Analysis for Biotherapeutics ... types and MSn capabilities that enable the user to push the boundaries of what is possible in glycan and glycopeptides analyses ... Second Generation Quadrupole Dual Cell Linear Ion Trap Orbitrap MS for Glycopeptide Analysis ...
... By Amanda Maxwell 03.18.2015 Wu and colleagues (2014) have published an ... pGlyco: An Intact Glycopeptide Identification Pipeline. Despite the promise of liquid chromatography-mass spec... by Melissa J ... Accelerating ScienceAccelerating Proteomics / Post-Translational Modifications / Advances in LC-MS Glycopeptide ... Novel Glycopeptides Identified Through Retrospective Analysis. The study of extracellular O-linked glycosylation is restric... ...
Amphipathic glycopeptides, the amino acid sequence of which comprises an N-terminal opioid message sequence, a C-terminal ... 2. The glycopeptide of claim 1, wherein the N-terminal opioid message sequence is Y-t-G-F- or Y-a-G-F-. 3. The glycopeptide of ... 5. The glycopeptide of claim 1, wherein the N-terminal opioid message sequence is Y-t-G-F-L-G-G-. 6. The glycopeptide of claim ... 8. The glycopeptide of claim 1, which adopts a helical conformation in the presence of a lipid bilayer. 9. The glycopeptide of ...
What CoNS species with decreased susceptibility to glycopeptides have been reported? Isolates of S. haemolyticus and S. ... The term glycopeptide refers to a group of antimicrobial agents that includes vancomycin, teicoplanin, and several other ... Why is the term "glycopeptide" used, instead of vancomycin, to describe the decreased susceptibility of these CoNS isolates? ... Because of its broader scope, the term glycopeptide is used instead of vancomycin to describe these isolates. CoNS isolates ...
Nowadays, glycopeptides have played a pivotal role in a myriad of organisms and systems, such as biology, physiology, medicine ... As is known, synthetic glycopeptides are able to offer an unique frontier for research in glycobiology and proteomics as well ... Technically, the Glycan chains of glycopeptides are involved in numerous biological recognition events, including protein ... has released its efficient Glycopeptide Synthesis service, to help speed up the advance in solid phase methods. ...
... Nat Methods. 2009 Nov;6(11):809-11. doi: ...
Mass spectrometric analysis of intact glycopeptides can reveal detailed information about glycosite, glycan structural features ... On the other hand, haptoglobin has both linked sialic acids on its glycopeptide, S1(2,6)S1(2,3)H5N4; similarly, glycopeptide ... e) The derivatized glycopeptides are enriched by HILIC SPE chromatography, and flow-through contains non-glycopeptides. (f) ... Other methods center on glycopeptide capture. Because each glycopeptide carries at least one glycan with vicinal hydroxyls, the ...
Selective enrichment of glycopeptides from complex samples prior to MS analysis is essential for successf ... However, the analysis of glycopeptides through MS remains challenging due to the microheterogeneity and macroheterogeneity of ... Study on behaviors and performances of universal N-glycopeptide enrichment methods Y. Xue, J. Xie, P. Fang, J. Yao, G. Yan, H. ... Selective enrichment of glycopeptides from complex samples prior to MS analysis is essential for successful glycoproteome ...
... a tool for the biosynthesis of modified glycopeptide antibiotics. ... Structure and function of the glycopeptide N-methyltransferase ...
35S-Glycosaminoglycan and 35S-Glycopeptide Metabolism by Diabetic Glomeruli and Aorta Message Subject (Your Name) has forwarded ... These studies suggest that synthesis of 35S-glycosaminoglycans and 35S-glycopeptides by diabetic glomeruli are altered by ... Both control and diabetic glomeruli synthesize 35S-labeled glycopeptides, the quantity from diabetic glomeruli being reduced. ... 35S-Glycosaminoglycan and 35S-Glycopeptide Metabolism by Diabetic Glomeruli and Aorta. ...
Glycopeptides and Lipoglycopeptides - Learn about the causes, symptoms, diagnosis & treatment from the Merck Manuals - Medical ... Use of Glycopeptides and Lipoglycopeptides During Pregnancy and Breastfeeding Use of glycopeptides and lipoglycopeptides during ... Glycopeptides and lipoglycopeptides are antibiotics used to treat complicated and/or serious infections caused by gram-positive ... Glycopeptides and lipoglycopeptides work by preventing gram-positive bacteria from forming this cell wall, resulting in the ...
UNIT 17.15B β-Elimination for Release of O-GalNAc-Linked Oligosaccharides from Glycoproteins and Glycopeptides. ... β-Elimination for Release of O-GalNAc-Linked Oligosaccharides from Glycoproteins and Glycopeptides. Current Protocols in ...
Scientists have found that cancer patients produce antibodies that target abnormal glycoproteins (proteins with sugar molecules attached) made by their tumors. The result of this work suggests that antitumor antibodies in the blood may provide a fruitful source of sensitive biomarkers for cancer detection. The study, supported in part by the National Cancer Institute (NCI), part of the National Institutes of Health, appears in the Feb. 15, 2010 issue of the journal Cancer Research.. ...
... Glycolipids are limited in fractions but known to be major and important contributors of ... Glycopeptides are basically peptides that contain carbohydrate moieties generally glycans which are covalently attached to the ...
Partial Deglycosylation of O-Glycopeptides (14). Sialic acid and β-galactose units of glycopeptides were removed by incubation ... and sialylated glycopeptides are retained longer. As a result, sialylated glycopeptides can be selectively isolated from ... HCD data of glycopeptides representing this sequence stretch revealed the presence of HexNAc2, i.e. not only mucin core-1, but ... Identification of glycopeptides first was carried out by database searching of HCD and ETD MS/MS data (acquired on the isolated ...
Table II Glycopeptides representing the same region, Ser154-Lys175 with variable glycosylation, identified from insulin-like ... How to Dig Deeper? Improved Enrichment Methods for Mucin Core-1 Type Glycopeptides. Z. Darula, J. Sherman and K. F. ... Distribution of glycopeptides from nine abundant proteins in the mixed-bed ion exchange separation fractions. Different site ... HCD (upper panel) and ETD (lower panel) spectra of the E1BCW0 glycopeptide, IQPPPT(HexNAc2)EALLTLPGPT(HexNAc)AAGPAGR. The ...
A number of recent advances in the chemical synthesis of glycopeptides and glycoproteins are described, with particular focus ... on the development of peptide ligation strategies and their implementation in the convergent assembly of complex glycopeptides ... glycopeptides. and glycoproteins R. J. Payne and C. Wong, Chem. Commun., 2010, 46, 21 DOI: 10.1039/B913845E ... glycopeptides. and glycoproteins are described, with particular focus on the development of peptide. ligation strategies and ...
Two viable approaches for optimizing known glycopeptide scaffolds have emerged--the chemical modification of existing scaffolds ... The present invention provides a strategy for glycopeptide optimization that involves chemoselective "neoglycosylation" ... 4 The lack of alternative therapeutics for VRE has increased interest in developing new glycopeptide derivatives to circumvent ... chemistry and uses this methodology to rapidly optimize teicoplanin-vancomycin hybrid glycopeptide antibiotics by exploringboth ...
... Author(s). Jovetic, S.; Yang Zhu, Yang; Marcone, G.L ... many of which are ever-evolving and resistant to nearly all available antibiotics beta-Lactams and glycopeptides are used to ...
This application note introduces a new method allowing for automatic identification of unknown glycopeptides in one step ... Protein glycosylation can effectively be analyzed on the glycopeptide level where site-specific modifications remain preserved ... MALDI-specific fragmentation patterns are used to successfully find the glycopeptide spectra in a complete LC-MALDI dataset and ... This application note introduces a new method allowing for automatic identification of unknown glycopeptides in one step ...
Timing of Initiating Glycopeptide Therapy for Methicillin-Resistant Staphylococcus aureus Bacteremia: The Impact on Clinical ... C. T. Fang, W. Y. Shau, P. R. Hsueh et al., "Early empirical glycopeptide therapy for patients with methicillin-resistant ... R. Leclercq and P. Courvalin, "Resistance to glycopeptides in enterococci," Clinical Infectious Diseases, vol. 24, no. 4, pp. ...
Timing of Initiating Glycopeptide Therapy for Methicillin-Resistant Staphylococcus aureus Bacteremia: The Impact on Clinical ... Chen-Hsiang Lee, Chun-Chih Chien, and Jien-Wei Liu, "Timing of Initiating Glycopeptide Therapy for Methicillin-Resistant ...
... peptides and glycopeptides, improving the current level of accuracy of intact glycopeptide identification. The N-glycoproteome ... Here the authors describe pGlyco 2.0, a workflow for the precise one step identification of intact N-glycopeptides at the ... Here, we propose a workflow for the precise high-throughput identification of intact N-glycopeptides at the proteome scale ... data quality and accessibility of intact glycopeptide identification lack behind those in routine proteomic analyses. ...
Glycopeptide chromatographic retention times were determined from a UPLC-MSE experiment. Since the common oxonium ions, e.g., m ... Improves the overall glycopeptide fragmentation efficiency. *SYNAPT G2-S HDMS System equipped with an ETD source proves to be ... Figure 4. Two additional ETD spectra from 4+ precursors of G1F (A) and G2F (B) containing glycopeptides. Super base m-NBA is ... CID fragmentation of glycopeptides is known to favor neutral losses (cleavage of regions within the attached sugar); ...
  • Generation of carbohydrate-specific marker ions during LC-ESMS of digested glycoproteins has been demonstrated to be a highly selective and sensitive approach for detection of glycopeptides. (nih.gov)
  • In this work, MRM was adopted for quantification of glycopeptides derived from both model glycoproteins and depleted human blood serum using glycan oxonium ions as transitions. (nih.gov)
  • A number of recent advances in the chemical synthesis of glycopeptides and glycoproteins are described, with particular focus on the development of peptide ligation strategies and their implementation in the convergent assembly of complex glycopeptides . (rsc.org)
  • SimGlycan also includes comprehensive support for resolving glycopeptides obtained from a LC-MS/MS run of proteolytically digested purified glycoproteins. (premierbiosoft.com)
  • Optimizing the recently developed Solid-Phase Extraction of (N-linked) Glycopeptides (SPEG) procedure for blood plasma analysis by spiking in [14C]-labeled human glycoproteins significantly enhanced the specificity and yield of the isolated N-linked glycopeptides. (slideserve.com)
  • After that, we applied the composite microspheres to profile the glycol-proteome of a normal human serum sample, 95 unique glycopeptides and 64 glycoproteins were identified with these enrichment substrates in a 5 μL of serum sample. (mdpi.com)
  • Using commercial glycoproteins, we demonstrated that in the presence of m-NBA the charge state of sialylated glycopeptides increased and the chromatographic separation of neutral and acidic glycopeptides revealed a remarkable improvement. (uzh.ch)
  • As to human serum samples, 41 glycoproteins and 66 glycopeptides corresponding to 68 N-glycosylation sites were finally detected, which showed the good ability of the nanosheets material to enrich N-linked glycopeptides from complex human serum samples. (ubc.ca)
  • Glycopeptides and Glycoproteins / Wittmann, Valentin (Hrsg. (uni-konstanz.de)
  • Glycopeptides and Glycoproteins : synthesis, structure, and application / vol. ed. (uni-konstanz.de)
  • Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level. (elsevier.com)
  • A rapid method to enrich intact glycopeptides is essential for the analysis of glycoproteins, especially for biopharmaceutical proteins. (elsevier.com)
  • These findings indicate that the site-specific characterization of glycoproteins from glycoengineered CHO-K1 cells can be achieved rapidly using the one-step intact glycopeptide enrichment method, which could provide insights for bio-analysts and biotechnologists to better tailor therapeutic drugs. (elsevier.com)
  • 1981) Characterization of the microheterogeneity in glycoproteins by 500 MHz [sup.1]H-NMR spectroscopy of glycopeptide preparations. (thefreedictionary.com)
  • Carbohydrate chemistry Glycopeptide antibiotic Glycosylation Peptide synthesis Talbot P. (wikipedia.org)
  • Mannopeptimycin glycopeptide is a lead antibiotic peptide. (wikipedia.org)
  • This unique mechanism of action, involving binding of the bulky inhibitor to the substrate outside the membrane so that the active sites of two enzymes cannot align themselves correctly, renders the acquisition of resistance to the glycopeptide antibiotics more difficult than that to the majority of the other antibiotic groups. (nih.gov)
  • VANCOMYCIN is a glycopeptide antibiotic. (goodrx.com)
  • Valine influences production and complex composition of glycopeptide antibiotic A40926 in fermentations of Nonomuraea sp. (biomedsearch.com)
  • ATCC 39727 the effect of valine on production of glycopeptide antibiotic A40926, which is a complex of factors structurally differing in fatty acid moieties. (biomedsearch.com)
  • The aim of this investigation was to explore the prevalence of MRSA and heterogeneous Glycopeptide Intermediate Staphylococcus Aureus (hGISA) in various clinical samples, to investigate the various antibiotic resistant determinant genes among these strains collected from north and west Indian hospitals and to evaluate the response of various drugs to these strains. (thescipub.com)
  • One hundred thirty-two glycopeptide-resistant Enterococcus faecium (GREF) isolates from different hospitals and pig and poultry farms in Belgium were compared on the basis of (i) their antibiotic susceptibilities, (ii) their SmaI pulsed-field gel electrophoresis (PFGE) patterns, and (iii) the organization of their Tn1546 or related elements in order to detect possible phenotypic and genotypic relationships among both groups of isolates. (ugent.be)
  • A new chiral stationary phase (CSP) was prepared by attachment of macrocyclic glycopeptide antibiotic eremomycin to the epoxy-activated silica under mild conditions. (edu.au)
  • The Mu50 glycopeptide-resistant S.aureus strain has reduced susceptibility to vancomycin, a glycopeptide antibiotic that is heavily relied on for the treatment of infections caused by S.aureus. (uncg.edu)
  • Avoparcin is a glycopeptide antibiotic with a gram positive spectrum of activity produced by fermentation of a strain of Streptomyces candidus. (thefreedictionary.com)
  • Vancomycin (Lilly's Vancocin, generics) is a narrow-spectrum, bactericidal glycopeptide antibiotic. (antiinfectivemeds.com)
  • Teicoplanin (Sanofi-Aventis' Targocid) is a narrow-spectrum, bactericidal glycopeptide antibiotic similar in structure to vancomycin. (antiinfectivemeds.com)
  • Wu and colleagues (2014) have published an improved mass spectrometry (MS) workflow with parallel data acquisition for efficient, sensitive identification and sequencing of intact glycopeptides. (thermofisher.com)
  • Currently with MS-based strategies, tandem MS (MS/MS) fragmentation and data analysis problems hamper efficient characterization of intact glycopeptides. (thermofisher.com)
  • Using Sweet-Heart, a suite of software tools allowing for automated MS/MS and MS 3 characterization of glycopeptides, the authors examined the CID spectra to establish glycoforms for intact glycopeptides. (thermofisher.com)
  • Mass spectrometric analysis of intact glycopeptides can reveal detailed information about glycosite, glycan structural features, and their heterogeneity. (springer.com)
  • The precise and large-scale identification of intact glycopeptides is a critical step in glycoproteomics. (nature.com)
  • In this study, we established a one-step method for the rapid capture of intact glycopeptides for analysis by mass spectrometry. (elsevier.com)
  • Compared to the conventional sequential enrichment method, the one-step intact glycopeptide enrichment method reduced the sample preparation time and improved the detection of intact glycopeptides with long sequences or non-polar amino acids. (elsevier.com)
  • Resolving this conundrum requires efficient and specific enrichment of intact glycopeptides for identification and quantitation. (nih.gov)
  • Mayampurath A, Yu CY, Song E, Balan J, Mechref Y, Tang H. Computational framework for identification of intact glycopeptides in complex samples. (uchicago.edu)
  • Analysis of the glycopeptides in these fractions by MS identified the specific glycosylation sites and enabled the prediction of the family of glycoforms at each attachment site. (nih.gov)
  • Analysis of protein glycosylation primarily includes identification of (1) glycosite, (2) glycopeptide sequence, and (3) resident glycans. (springer.com)
  • Protein glycosylation can effectively be analyzed on the glycopeptide level where site-specific modifications remain preserved. (selectscience.net)
  • Owing to the complexity of glycosylation, the current overall throughput, data quality and accessibility of intact glycopeptide identification lack behind those in routine proteomic analyses. (nature.com)
  • Furthermore, comprehensive support for resolving glycopeptides using LC-MS/MS glycopeptide data facilitates glycosylation studies. (premierbiosoft.com)
  • In consideration of IgA nephropathy in which the hinge region is incompletely glycosylated, we used this hinge sequence that encodes five potential glycosylation sites as the ideal template for the synthesis of a Tn antigen-expressing glycopeptide. (uva.nl)
  • Here, a chemoenzymatic method is introduced for the site‐specific extraction of O‐linked glycopeptides (EXoO), which enabled the mapping of over 3,000 O‐linked glycosylation sites and definition of their glycans on over 1,000 proteins in human kidney tissues, T cells, and serum. (embopress.org)
  • Mapping of these epitopes relies on a novel three-step procedure that identifies any reactive viral O-glycosyl peptide epitope with respect to (i) relevant peptide sequence, (ii) the reactive glycoform out of several possible glycopeptide isomers of that peptide sequence, and (iii) possibly tolerated carbohydrate or peptide structural variations at glycosylation sites. (ku.dk)
  • The fact that a single mutation in the amino acid sequence of biosimilar can cause a significant change in the resulting glycosylation patterns and/or physiochemical properties emphasizes the importance of detailed glycopeptide mapping methods, such as LC-tandem mass spectrometry-based method as described our experiment, which can distinguish even slight variations on the minor glycoform species as an added accuracy in biosimilar development. (thefreedictionary.com)
  • Targeted Measurements of O- and N-Glycopeptides Show That Proteins in High Density Lipoprotein Particles Are Enriched with Specific Glycosylation Compared to Plasma. (uchicago.edu)
  • Mass spectrometric analysis of glycopeptides is an emerging strategy for analysis of glycosylation patterns. (elsevier.com)
  • We show that glycosylation does not affect peptide binding to MHC class I and that glycopeptides can elicit a strong CTL response that is glycopeptide specific. (ox.ac.uk)
  • Glycopeptide antibiotics, including vancomycin and teicoplanin, are large, rigid molecules that inhibit a late stage in bacterial cell wall peptidoglycan synthesis. (nih.gov)
  • Glycopeptides, such as vancomycin and teicoplanin, are primarily used in the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections, such as cellulitis, endocarditis, meningitis, pneumonia, and septicemia, and are some of the most commonly prescribed parenteral antimicrobials. (mdpi.com)
  • The antibacterial efficacies of the beta-lactam antibiotics nafcillin and cefazolin were compared with those of the glycopeptide antibiotics vancomycin and teicoplanin in rats with subcutaneous abscesses infected with methicillin-susceptible Staphylococcus aureus. (asm.org)
  • The vanA gene contributes higher resistance to vancomycin and teicoplanin both glycopeptides while VanB gene which is also an effective resistant gene encodes resistance to vancomycin only. (thefreedictionary.com)
  • Structure, biochemistry and mechanism of action of glycopeptide antibiotics. (nih.gov)
  • Glycopeptides and lipoglycopeptides are antibiotics used to treat complicated and/or serious infections caused by gram-positive bacteria . (merckmanuals.com)
  • beta-Lactam and glycopeptide antibiotics: first and last line of defense? (wur.nl)
  • The beta-lactam antibiotics were more effective therapy than the glycopeptide antibiotics in rats with subcutaneous abscesses infected with methicillin-susceptible S. aureus. (asm.org)
  • Glycopeptide Antibiotics Market Undertakes Strapping Grow. (pharmiweb.com)
  • Global Glycopeptide Antibiotics Market report brings into light key market dynamics of the sector. (pharmiweb.com)
  • While formulating this Glycopeptide Antibiotics Market analysis report, client business competence is understood adeptly to identify tangible growth opportunities. (pharmiweb.com)
  • The wide ranging Glycopeptide Antibiotics Market report evaluates CAGR value fluctuation during the forecast period of 2021-2028 for the market. (pharmiweb.com)
  • Base year for calculation in the report is considered as 2020 while the historic year is 2019 which will tell how the Glycopeptide Antibiotics Market is going to act upon in the forecast years by giving information about the several market insights. (pharmiweb.com)
  • Global glycopeptide antibiotics market is expected to gain market growth in the forecast period of 2020 to 2027. (pharmiweb.com)
  • The growth of glycopeptide antibiotics market enhanced by the growing cases of infectious diseases and rise in research and development activities conducted by many pharmaceuticals' companies. (pharmiweb.com)
  • In addition, increase patient awareness level, advances in the treatment options and adoption of sedentary life-style are some of the impacting factors for the growth of glycopeptide antibiotics market. (pharmiweb.com)
  • Glycopeptide antibiotics are the class of antibiotics that inhibits the bacterial cell wall formation by blocking the peptidoglycan synthesis. (pharmiweb.com)
  • Glycopeptide antibiotics are widely used in the treatment of infectious diseases. (pharmiweb.com)
  • Glycopeptide antibiotics are act as a bacteriostatic and greatly effective against drug resistant bacteria. (pharmiweb.com)
  • Glycopeptide antibiotics market provides details of market share, new developments and product pipeline analysis, impact of domestic and localized market players, analyses opportunities in terms of emerging revenue pockets, changes in market regulations, product approvals, strategic decisions, product launches, geographic expansions and technological innovations in the market. (pharmiweb.com)
  • Glycopeptide antibiotics market is segmented on the basis of drugs, indication, route of administration end-users and distribution channel. (pharmiweb.com)
  • Based on drugs, the global glycopeptide antibiotics market is segmented into vancomycin, dalbavancin, oritavancin and others. (pharmiweb.com)
  • Indication section of global glycopeptide antibiotics market is segmented into skin and structure infection, meningitis, clostridioides difficile Infection and others. (pharmiweb.com)
  • Route of administration segment for global glycopeptide antibiotics market is categorized into oral, parenteral and others. (pharmiweb.com)
  • On the basis of end-users, the global glycopeptide antibiotics market is segmented into hospitals, homecare, specialty clinics and others. (pharmiweb.com)
  • It was demonstrated by the example of ristocetin A CSP that method of the immobilization of macrocyclic glycopeptide antibiotics affects remarkably the resulting enantioselectivity. (edu.au)
  • The two component system GraSR genes from Mu50 glycopeptide-resistant S.aureus have been identified as a mediator in a rapid signal transduction cascade that occurs when the bacteria cell wall is stressed by antibiotics. (uncg.edu)
  • Glycopeptide drugs that bind to nascent PG (incompletely cross-linked PG that is just entering the lattice) interfere with the ordering of the structural motif and are therefore potent antibiotics. (umich.edu)
  • Agarwal R, Bartsch SM, Kelly BJ, Prewitt M, Liu Y, Chen Y, Umscheid CA. Newer glycopeptide antibiotics for treatment of complicated skin and soft tissue infections: systematic review, network meta-analysis and cost analysis. (uchicago.edu)
  • This course examines the medication family known as glycopeptide antibiotics, and the various classes of drugs within this family. (pedagogyeducation.com)
  • 3. Identify the classifications of intravenous glycopeptide antibiotics and medications belonging to each class. (pedagogyeducation.com)
  • The term glycopeptide refers to a group of antimicrobial agents that includes vancomycin, teicoplanin, and several other antimicrobial agents currently in U. S. clinical trials. (cdc.gov)
  • No vancomycin-resistant S. aureus or glycopeptide-intermediate S. aureus (GISA) were detected but 178 isolates were confirmed by vancomycin population analysis profile (PAP)-area under the curve (vPAP-AUC) ratio determination and/or teicoplanin PAP (tPAP) methods as hetero-GISA (hGISA). (tcd.ie)
  • Heterogeneous resistance to glycopeptide in CoNS strains was observed to be significantly more emergent than those of S. aureus strains (vancomycin P 0.001, teicoplanin, P 0.007). (biomedcentral.com)
  • Early empirical glycopeptide therapy for patients with methicillin-resistant Staphylococcus aureus bacteraemia: impact on the outcome," Journal of Antimicrobial Chemotherapy , vol. 57, no. 3, pp. 511-519, 2006. (hindawi.com)
  • Chen-Hsiang Lee, Chun-Chih Chien, and Jien-Wei Liu, "Timing of Initiating Glycopeptide Therapy for Methicillin-Resistant Staphylococcus aureus Bacteremia: The Impact on Clinical Outcome," The Scientific World Journal , vol. 2013, Article ID 457435, 7 pages, 2013. (hindawi.com)
  • Strains of Staphylococcus aureus with reduced susceptibility to glycopeptides have been reported from Japan, the United States, Europe, and the Far East. (cdc.gov)
  • Beta-lactams versus glycopeptides in treatment of subcutaneous abscesses infected with Staphylococcus aureus. (asm.org)
  • Meticillin-resistant Staphylococcus aureus (MRSA) isolates (n=3,189) from 2990 patients were studied by agar screening and the E-test™ macromethod to investigate reduced susceptibility to glycopeptides among six collections of MRSA recovered between 1998 and 2004. (tcd.ie)
  • The hetero-glycopeptide intermediate staphylococci is considered to be the precursor of glycopeptide intermediate staphylococci especially vancomycin intermediate Staphylococcus aureus (VISA). (biomedcentral.com)
  • For this purpose, we aimed to investigate the heterogeneous resistance to glycopeptide and their frequencies in 135 Staphylococcus strains. (biomedcentral.com)
  • Eight (6%) out of 135 Staphylococcus strains were exhibited heterogeneous resistance to at least one glycopeptide. (biomedcentral.com)
  • The results indicate that oxonium ions could be adopted for the characterization and quantification of glycopeptides in general, eliminating the need to select specific transitions for individual precursor ions. (nih.gov)
  • Both methods led to at least 65% of the identified products being glycopeptides, in comparison to ∼25% without the additional chromatography steps [Darula, Z., and Medzihradszky, K. F. (2009) Affinity enrichment and characterization of mucin core-1 type glycopeptides from bovine serum. (mcponline.org)
  • In order to improve not only the isolation but also the characterization of the glycopeptides exoglycosidases were used to eliminate carbohydrate extensions from the directly peptide-bound GalNAc units. (mcponline.org)
  • This application note offers details of glycopeptide characterization using ETD fragmentation. (waters.com)
  • Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147. (asm.org)
  • Characterization of glycopeptides, aminoglycosides and macrolide resistance among Enterococcus faecalis and Enterococcus faecium isolates from hospitals in Tehran. (semanticscholar.org)
  • The synthesis of glycopeptides provides biological probes for researchers to elucidate glycan function in nature and products that have useful therapeutic and biotechnological applications. (wikipedia.org)
  • Several methods have been reported in the literature for the synthesis of glycopeptides. (wikipedia.org)
  • Within solid phase peptide synthesis (SPPS) there exist two strategies for the synthesis of glycopeptides, linear and convergent assembly. (wikipedia.org)
  • Linear assembly remains a popular strategy for the synthesis of glycopeptides with many examples in the literature. (wikipedia.org)
  • All clinical isolates with decreased susceptibility to glycopeptides have been oxacillin resistant and resistant to many other commonly used therapeutic agents. (cdc.gov)
  • Like oxacillin-resistant CoNS, isolates with decreased susceptibility to glycopeptides can show heteroresistance. (cdc.gov)
  • What CoNS species with decreased susceptibility to glycopeptides have been reported? (cdc.gov)
  • Isolates of S. haemolyticus and S. epidermidis with decreased susceptibility to glycopeptides have been reported. (cdc.gov)
  • Not all routine susceptibility test methods can detect CoNS isolates with decreased susceptibility to glycopeptides. (cdc.gov)
  • Should CoNS with decreased susceptibility to glycopeptides be reported to infection control? (cdc.gov)
  • Many of the isolates with reduced susceptibility to glycopeptides have been associated with therapeutic failures with vancomycin. (cdc.gov)
  • As a consequence, selective pressure was established that eventually led to the emergence of strains of S. aureus and other species of staphylococci with decreased susceptibility to vancomycin and other glycopeptides. (cdc.gov)
  • In vitro, animal, and clinical data comparing beta-lactam and glycopeptide treatment of S. aureus infections were reviewed along with the resulting treatment recommendations. (asm.org)
  • aureus isolates and glycopeptide resistance was not found. (thefreedictionary.com)
  • We suggested to screen clinical S. aureus and CoNS strains, systematically, for the presence of heterogeneously resistance to glycopeptide. (biomedcentral.com)
  • Distribution of glycopeptides from nine abundant proteins in the mixed-bed ion exchange separation fractions. (mcponline.org)
  • This assay was based on specific amplification of internal fragments of genes encoding D-alanine:D-alanine ligases and related glycopeptide resistance proteins. (asm.org)
  • Oxygen-linked glycopeptides and proteins are quite prevalent. (novoprolabs.com)
  • Here, the coupling efficiency for different NaIO4 oxidation conditions were determined by either direct [14C]-radioactivity assay of uncoupled fractions post coupling (A) or captured N-linked glycopeptides quantified by LC-MS/MS and isotopic-labeling (B). (slideserve.com)
  • A PCR assay that allows simultaneous detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci (Enterococcus faecium, E. faecalis, E. gallinarum, and E. casseliflavus) was developed. (asm.org)
  • Inducible and constitutive expression of resistance to glycopeptides and vancomycin dependence in glycopeptide-resistant Enterococcus avium. (asm.org)
  • Descheemaeker P, Chapelle S, Devriese L, Butaye P, Vandamme P, Goossens H. Comparison of glycopeptide-resistant Enterococcus faecium isolates and glycopeptide resistance genes of human and animal origins. (ugent.be)
  • The prevalence of glycopeptides, aminoglycosides and erythromycin resistance among Enterococcus faecalis and Enterococcus faecium was investigated. (semanticscholar.org)
  • The team acquired data for analysis from both the CID spectra and the ETD MS/MS data, using the latter for direct glycopeptide identification. (thermofisher.com)
  • HCD ( upper panel) and ETD ( lower panel) spectra of the E1BCW0 glycopeptide, IQPPPT(HexNAc 2 )EALLTLPGPT(HexNAc)AAGPAGR. (mcponline.org)
  • MALDI-specific fragmentation patterns are used to successfully find the glycopeptide spectra in a complete LC-MALDI dataset and identify glycan and peptide moieties. (selectscience.net)
  • Only glycopeptide spectra are submitted to the GlycoQuest search for glycan identification. (bruker.com)
  • Nonetheless, data analysis is a major bottleneck to high throughput glycopeptide identification based on ETD data, due to the complexity and diversity of ETD mass spectra compared to CID counterparts. (ku.edu)
  • It filters out noise peaks that interfere with glycopeptide sequencing, correlates input glycopeptide compositions with the ETD spectra, and assigns a score for each candidate. (ku.edu)
  • Here we present an approach using energy resolved collision induced dissociation (CID) spectra to determine structural features of glycopeptides. (elsevier.com)
  • Glycopeptides are peptides that contain carbohydrate moieties (glycans) covalently attached to the side chains of the amino acid residues that constitute the peptide. (wikipedia.org)
  • Minyong and Jim summarize their recent Nature Communications publication describing selective capture of N-glycans and N-glycopeptides by an engineered high affinity Fbs1 carbohydrate binding protein. (neb.com)
  • Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. (jic.ac.uk)
  • Glycopeptide has been widely recognized as peptide that contains carbohydrate moieties (glycans). (novoprolabs.com)
  • Recognition of carbohydrate by major histocompatibility complex class I-restricted, glycopeptide-specific cytotoxic T lymphocytes. (ox.ac.uk)
  • Using these optimized conditions, marker ion detection and glycopeptide analysis could be achieved with as little as 10 pmol of a glycoprotein. (nih.gov)
  • However, the analysis of glycopeptides through MS remains challenging due to the microheterogeneity and macroheterogeneity of the glycoprotein. (rsc.org)
  • 1 Pulling together the enhanced specifications of the new Orbitrap Fusion Tribrid mass spectrometer from Thermo Scientific, the researchers present a product-dependent, parallel data-acquisition and data-analysis workflow that reduces false positives, in addition to accurately identifying the glycopeptide backbone. (thermofisher.com)
  • 2 They modified the software for HCD analysis to uncover the Y1-based glycopeptide backbone for classification. (thermofisher.com)
  • They found that HCD was better for matching glycopeptide identities than ETD, because the technique produced greater quantities of glyco-oxonium ions for onward analysis in the CID/ETD MS/MS step. (thermofisher.com)
  • propose that linking the preceding HCD acquisition step to restrict onward CID analysis to glycopeptides only was a key advantage offered by the Orbitrap Fusion Tribrid instrumentation. (thermofisher.com)
  • They suggest that integration of ETD data with improvements in scoring algorithms would enhance data output as a step forward in MS-based proteomic analysis of glycopeptides. (thermofisher.com)
  • Physiochemical analysis and CNS bioavailability of opioid amphipathic glycopeptides. (freepatentsonline.com)
  • Alternatively, the sialyl glycopeptides can be identified using positive-ion mode analysis by doping a high concentration of sodium salts to the analytes. (springer.com)
  • Selective enrichment of glycopeptides from complex samples prior to MS analysis is essential for successful glycoproteome research. (rsc.org)
  • The intact N -glycopeptides were interpreted using the in-house analysis software pGlyco 2.0. (rsc.org)
  • Glycopeptide analysis allows the site-specific analysis of glycans. (bruker.com)
  • ProteinScape supports the analysis of glycopeptide data from other instruments as well. (bruker.com)
  • MS/MS fragmentation patterns of glycans and glycopeptides (based on different instrument settings) can be specified in order to enable the program to reduce false positives from the data analysis results. (premierbiosoft.com)
  • Recently, we have developed a method for Solid-Phase Extraction of N-linked Glycopeptides (SPEG), and we have shown that analysis of plasma using SPEG improves dynamic range and sensitivity. (slideserve.com)
  • Biophysical analysis of the ability of both "Click" and native glycopeptides peptides with recombinant MGL confirmed the ability of both these peptides to bind MGL. (auckland.ac.nz)
  • In this study, we demonstrate that the addition of supercharging reagent, m-nitrobenzyl alcohol (m-NBA), into mobile phases greatly facilitates the analysis of acidic and hMW glycopeptides. (uzh.ch)
  • It also performed a high sensitivity (1 × 10−10 M HRP), a good enrichment capacity (250 mg/g) for glycopeptides enrichment and a high enrichment recovery of 78%, suggesting a great application prospect for the enrichment of N-linked glycopeptides and the analysis glycoproteomic research. (ubc.ca)
  • Agar dilution method was used for determining MICs of glycopeptides and population analysis profile was performed for detecting frequency of heterogeneous resistance for the parents of selected strains on BHA-4. (biomedcentral.com)
  • As a result of binding to L-aa-D-Ala-D-Ala groups in wall intermediates, glycopeptides inhibit, apparently by steric hindrance, the formation of the backbone glycan chains (catalysed by peptidoglycan polymerase) from the simple wall subunits as they are extruded through the cytoplasmic membrane. (nih.gov)
  • While vancomycin therapy appeared to have failed in the patients infected with these organisms, debate was considerable about whether such strains should be designated as resistant to glycopeptides, since the levels of vancomycin required to inhibit the growth of the strains remained low (vancomycin MIC = 8 µg/mL). (cdc.gov)
  • Glycopeptides inhibit bacterial cell-wall biosynthesis by blocking glycopeptide polymerization. (antiinfectivemeds.com)
  • Glycopeptides also alter the permeability of the cytoplasmic membrane and directly inhibit RNA synthesis. (antiinfectivemeds.com)
  • SimGlycan predicts the structure of glycans and glycopeptides using mass spectrometry data. (premierbiosoft.com)
  • pGlyco 2.0 conducts comprehensive quality control including false discovery rate evaluation at all three levels of matches to glycans, peptides and glycopeptides, improving the current level of accuracy of intact glycopeptide identification. (nature.com)
  • Second, comprehensive quality control has not been developed and integrated into search engines for intact glycopeptide identification, with a false discovery rate (FDR) evaluation needed on all three levels of matches to glycans, peptides and glycopeptides. (nature.com)
  • Antifreeze peptides and glycopeptides, and their derivatives: potential uses in biotechnology. (uni-bielefeld.de)
  • Synthesis of peptides and glycopeptides with polyproline II helical topology as potential antifreeze molecules. (uni-bielefeld.de)
  • Native chemical ligation (NCL) is a convergent synthetic strategy based on the linear coupling of glycopeptide fragments. (wikipedia.org)
  • To address these limitations, we performed extensive analyses and developed novel methods, including a high-throughput MS acquisition method based on optimized MS/MS collision parameters, which generates comprehensive fragments of an intact glycopeptide in a single spectrum. (nature.com)
  • Spectrum viewer showing the annotation of glycan and peptide fragments of a glycopeptide (click to enlarge). (bruker.com)
  • Tolbert T.J., Wong CH. (2004) Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments. (springer.com)
  • 2013) " Sweet-Heart: An integrated suite of enabling computational tools for automated MS2/MS3 sequencing and identification of glycopeptides ," Journal of Proteomics, 84 (pp. 1-16). (thermofisher.com)
  • As is known, synthetic glycopeptides are able to offer an unique frontier for research in glycobiology and proteomics as well as for drug discovery & development, drug delivery & targeting, diagnostics development and biotechnological applications, which also promotes the development of modern biomarker discovery process. (lifeboat.com)
  • Why is the term "glycopeptide" used, instead of vancomycin, to describe the decreased susceptibility of these CoNS isolates? (cdc.gov)
  • Because of its broader scope, the term glycopeptide is used instead of vancomycin to describe these isolates. (cdc.gov)
  • This method describes the conjugation of a synthetic glycopeptide to the N-terminus of a recombinant human interleukin-2 (IL-2) protein fragment. (springer.com)
  • These include the following: solid phase derivatization, enhanced ionization of sialoglycopeptides, differentiation of sialic acid linkage, and enrichment of the modified glycopeptides by hydrophilic interaction liquid chromatography. (springer.com)
  • Comparison of Enrichment Methods for Intact N- and O-Linked Glycopeptides Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography. (nih.gov)
  • Seventeen N-linked glycopeptides from horseradish peroxidase (HRP) tryptic digests were readily identified and the results showed highly improved signal intensities. (ubc.ca)
  • In this work, we systematically investigated the behaviors and performances of boronic acid chemistry, ZIC-HILIC, and PGC of glycopeptide enrichment to promote understanding of these methods. (rsc.org)
  • We also optimized boronic acid chemistry and ZIC-HILIC enrichment methods and applied them to enrich glycopeptides from mouse liver. (rsc.org)
  • We found that boronic acid chemistry in this study preferred to capture glycopeptides with high mannose glycans, ZIC-HILIC enriched most N -glycopeptides and did not show significant preference during enrichment and PGC was not suitable for separating glycopeptides with a long amino acid sequence. (rsc.org)
  • Synthetic glycopeptides were produced incorporating either native O-linked GalNAc or GalNAc residues linked to the peptide chain via non-native "Click" chemistry. (auckland.ac.nz)
  • Mizuno, M., Haneda, K., and Iguchi, R. (1999) Synthesis of a glycopeptide containing oligosaccharides: chemoenzymic synthesis of eel calcitonin analogs having natural N-linked oligosaccharides. (springer.com)
  • Glycopeptides and lipoglycopeptides work by preventing gram-positive bacteria from forming this cell wall, resulting in the death of the bacteria. (merckmanuals.com)
  • Bovine serum samples were subjected to lectin affinity-chromatography both at the protein- and peptide-level in order to selectively isolate glycopeptides with the most common, mucin core-1 sugar. (mcponline.org)
  • Solid-phase synthesis of mucin glycopeptides is an effective way to prepare constructs in order to investigate their biological and biophysical properties. (umn.edu)
  • Mucin-type glycopeptide structure in solution: past, present, and future. (uni-bielefeld.de)
  • MRM experiments were optimized by evaluating different parameters that have a major influence on quantification of glycopeptides, which include MRM time segments, number of transitions, and normalized collision energies. (nih.gov)
  • Antifreeze glycopeptide analogues: microwave-enhanced synthesis and functional studies", Amino Acids , vol. 38, 2010, pp. 213-222. (uni-bielefeld.de)
  • Make use of the high glycopeptide sensitivity using the Captive Spray nanoBooster for high glycopeptide sensitivity, which improves ionization efficiency. (bruker.com)
  • 14C]-Radioactivity assay offers very similar pattern of coupling efficiency comparing with N-linked glycopeptides quantified with LC-MS/MS and isotopic labeling. (slideserve.com)
  • A hydrophilic two-dimensional titanium-based metal-organic framework (MOF) nanosheets were prepared and applied to improve the glycopeptides enrichment efficiency. (ubc.ca)
  • GlycoPep Grader (GPG) is a freely-available software tool designed to accelerate the process of accurately determining glycopeptide composition from tandem mass spectrometric data. (ku.edu)
  • Here, we propose a workflow for the precise high-throughput identification of intact N-glycopeptides at the proteome scale using stepped-energy fragmentation and a dedicated search engine. (nature.com)
  • The specificity of the assay was tested on 5 well-characterized glycopeptide-resistant strains and on 15 susceptible enterococcal type strains. (asm.org)
  • Southern hybridization and DNA amplification revealed the presence of Tn1546-related elements in enterococci highly resistant to glycopeptides. (asm.org)
  • Phenotypic detection of Glycopeptide resistance among Methicillin Resistant Staphylococci by various methods showed one isolate to be Vancomycin Intermediate S.epidermidis (VISE) from blood sample. (thefreedictionary.com)
  • The three-dimensional structure contains a cleft into which peptides of highly specific configuration (L-aa-D-aa-D-aa) can fit: such sequences are found only in bacterial cell walls, hence glycopeptides are selectively toxic. (nih.gov)
  • Technically, the Glycan chains of glycopeptides are involved in numerous biological recognition events, including protein folding, cell-cell communication and adhesion, cell growth and differentiation, as well as bacterial and viral infection. (lifeboat.com)
  • MS-based glycoproteomic studies accurately characterize the glycopeptides under examination. (thermofisher.com)
  • Selective detection of glycopeptides on ion trap mass spectrometers. (nih.gov)
  • The Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer offers unique acquisition strategies and workflows through its flexibility in fragmentation types and MS n capabilities that enable the user to push the boundaries of what is possible in glycan and glycopeptides analyses. (thermofisher.com)
  • To detect sialoglycopeptides, a negative-ion mode mass spectrometry may be applied with a minimal loss of sialic acids, although the positively charged or neutral glycopeptides may be excluded. (springer.com)
  • SimGlycan assists in predicting the structure of a glycan from the released glycan MS/MS data or directly a glycopeptide (peptide chain modified with a single or two glycan structures) from glycopeptide MS/MS data acquired using various ionization techniques of mass spectrometry. (premierbiosoft.com)
  • Electron transfer dissociation (ETD) is commonly used in fragmenting N-linked glycopeptides in their mass spectral analyses to complement collision induced dissociation (CID) experiments. (ku.edu)
  • It was found that removal of N-glycans prior to enrichment of O-linked glycopeptides by HILIC improved identification of O-linked glycopeptides by mass spectrometry. (nih.gov)
  • Fragmentation of multiply protonated glycopeptides proceeds by a series of competing charge separation processes by cleavage of a glycosidic bond, each producing two charged products: a singly charged, "B" type sugar (oxonium) ion, and a complementary high mass fragment. (elsevier.com)
  • Such as, the identification and location of glycopeptides. (thermofisher.com)
  • including site-specific glycopeptide identification. (thermofisher.com)
  • This method utilizes several components to facilitate glycopeptide identification. (springer.com)
  • This application note introduces a new method allowing for automatic identification of unknown glycopeptides in one step without prior release of the glycans. (selectscience.net)
  • The N-glycoproteome of samples metabolically labeled with 15 N/ 13 C were analyzed quantitatively and utilized to validate the glycopeptide identification, which could be used as a novel benchmark pipeline to compare different search engines. (nature.com)
  • Therefore, the overall throughput, the data quality and accessibility of intact glycopeptide identification are considerably lower than those of routine proteomic studies. (nature.com)
  • This approach provides and complete and simple solution for glycopeptide identification. (bruker.com)
  • Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. (asm.org)
  • We also compared the enrichment of intact N- and O-linked glycopeptides using other chromatography methods and found that using cartridges containing materials for strong anion exchange (SAX) chromatography increased yield and identification of N- and O-linked glycopeptides. (nih.gov)
  • Amphipathic glycopeptides, the amino acid sequence of which comprises an N-terminal opioid message sequence, a C-terminal helical address sequence, and a linker sequence between the message sequence and the helical address sequence, where the C-terminal helical address sequence has a length of nine amino acids, and at least one of the amino acid residues of the peptide is glycosylated. (freepatentsonline.com)
  • The Protein ID, Protein sequence or peptide sequences identified by a third party tool will be used as initial input in SimGlycan in order to identify the glycopeptides from data dependent MS/MS data. (premierbiosoft.com)
  • The glycan remains intact through ETD, while the peptide backbone is cleaved, providing the sequence of amino acids for a glycopeptide. (ku.edu)
  • To this end, hydrophilic interaction chromatography (HILIC) has been commonly used to enrich intact N- and O-linked glycopeptides. (nih.gov)
  • The increase MICs of glycopeptide resistance for subpopulations of staphylococci comparing with their parents could be an important clue for recognizing the early steps in the appearance of VISA strains. (biomedcentral.com)
  • The main difference between the VISA and VRSA strains is that the later could transfer the glycopeptide resistance among the strains by plasmids. (biomedcentral.com)
  • The glycopeptides are produced by spotting FMOC protected amino acids allowing the synthesis to be performed at microgram (nanomole) scale using very small amounts of glycoamino acids. (wikipedia.org)