Pathological processes of the PANCREAS.
Electrophoresis in which cellulose acetate is the diffusion medium.
Mucopolysaccharidosis characterized by excessive dermatan and heparan sulfates in the urine and Hurler-like features. It is caused by a deficiency of beta-glucuronidase.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
The failure by the observer to measure or identify a phenomenon accurately, which results in an error. Sources for this may be due to the observer's missing an abnormality, or to faulty technique resulting in incorrect test measurement, or to misinterpretation of the data. Two varieties are inter-observer variation (the amount observers vary from one another when reporting on the same material) and intra-observer variation (the amount one observer varies between observations when reporting more than once on the same material).
A group of hydroxycorticosteroids bearing a hydroxy group at the 17-position. Urinary excretion of these compounds is used as an index of adrenal function. They are used systemically in the free alcohol form, but with esterification of the hydroxy groups, topical effectiveness is increased.
A group of corticosteroids bearing a hydroxy group at the 11-position.
A group of corticosteroids carrying hydroxy groups, usually in the 11- or 17-positions. They comprise the bulk of the corticosteroids used systemically. As they are relatively insoluble in water, salts of various esterified forms are often used for injections or solutions.
An arylsulfatase with high specificity towards sulfated steroids. Defects in this enzyme are the cause of ICHTHYOSIS, X-LINKED.
A metabolite of TESTOSTERONE or ANDROSTENEDIONE with a 3-alpha-hydroxyl group and without the double bond. The 3-beta hydroxyl isomer is epiandrosterone.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Proteins obtained from ESCHERICHIA COLI.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Infections with bacteria of the species ESCHERICHIA COLI.
The segregation and degradation of damaged or unwanted cytoplasmic constituents by autophagic vacuoles (cytolysosomes) composed of LYSOSOMES containing cellular components in the process of digestion; it plays an important role in BIOLOGICAL METAMORPHOSIS of amphibians, in the removal of bone by osteoclasts, and in the degradation of normal cell components in nutritional deficiency states.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
An enzyme that hydrolyzes iduronosidic linkages in desulfated dermatan. Deficiency of this enzyme produces Hurler's syndrome. EC 3.2.1.76.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Group of lysosomal storage diseases each caused by an inherited deficiency of an enzyme involved in the degradation of glycosaminoglycans (mucopolysaccharides). The diseases are progressive and often display a wide spectrum of clinical severity within one enzyme deficiency.
A flavonol widely distributed in plants. It is an antioxidant, like many other phenolic heterocyclic compounds. Glycosylated forms include RUTIN and quercetrin.
A publication issued at stated, more or less regular, intervals.
Glycosides of GLUCURONIC ACID formed by the reaction of URIDINE DIPHOSPHATE GLUCURONIC ACID with certain endogenous and exogenous substances. Their formation is important for the detoxification of drugs, steroid excretion and BILIRUBIN metabolism to a more water-soluble compound that can be eliminated in the URINE and BILE.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A species of GRAM-POSITIVE ENDOSPORE-FORMING BACTERIA in the family BACILLACEAE, found in soil, hot springs, Arctic waters, ocean sediments, and spoiled food products.
A family of bacteria which produce endospores. They are mostly saprophytes from soil, but a few are insect or animal parasites or pathogens.
A genus of gram-positive, endospore-forming, thermophilic bacteria in the family BACILLACEAE.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)
Fishman, William H.; Bernfeld, Peter (1955). [31] Glucuronidases. Methods in Enzymology. 1. pp. 262-9. doi:10.1016/0076-6879(55 ...
Akao, Taiko; Akao, Teruaki; Kobashi, Kyoichi (1987). "Glycyrrhizin .BETA.-D-glucuronidase of Eubacterium sp. from human ...
... beta-glucuronidase (EC 3.2.1.31). These enzymes contain a conserved glutamic acid residue which has been shown, in Escherichia ...
... (EC 3.2.1.131, 1,2-alpha-glucuronidase, alpha-(1->2)-glucuronidase, xylan alpha-D-1,2-(4-O- ... Glucuronidase in the enzymatic saccharification of hardwood xylan .1. Screening of α-glucuronidase producing fungi". Mokuzai ...
Skin cells and red blood cells of affected people will have low levels of β-glucuronidase activity. Sly syndrome can also be ... Allanson, JE; Gemmill, RM; Hecht, BK; Johnsen, S; Wenger, DA (1988). "Deletion mapping of the beta-glucuronidase gene". ... Sly WS, Quinton BA, McAlister WH, Rimoin DL (1973). "Beta glucuronidase deficiency: report of clinical, radiologic, and ... an enzyme replacement therapy which is a recombinant form of human β-glucuronidase, is approved by U.S. Food and Drug ...
"Activity-based probes for functional interrogation of retaining β-glucuronidases" (PDF). Nat. Chem. Biol. 13 (8): 867-873. doi: ...
Enzymes that cleave the glycosidic bond of a glucuronide are called glucuronidases. Miquelianin (Quercetin 3-O-glucuronide) ...
It is an inhibitor of the enzyme β-glucuronidase. Merck Index, 11th Edition, 20 Glucaron, drugs.com Iida R, Nagata S, Kakimoto ... M, Akaike H, Watanabe H, Shioya A (1965). "2,5-Di-O-acetyl-D-glucosaccharo-(1→4)(6→3)-dilactone, a new potent β-glucuronidase ...
I. Sulphatase and beta-glucuronidase activity of molluscan extracts". The Biochemical Journal. 63 (4): 705-10. doi:10.1042/ ...
Moszczyński P, Lisiewicz J (1984). "Beta-glucuronidase of lymphocytes in ontogenetic development of man". Arch. Immunol. Ther. ...
Dietary L-aspartic acid has been shown to act as an inhibitor of Beta-glucuronidase, which serves to regulate enterohepatic ... ISBN 1-57259-153-6. Kreamer, Siegel, & Gourley (Oct 2001). "A novel inhibitor of beta-glucuronidase: L-aspartic acid". ...
Kim, DH; Shim, SB; Kim, NJ; Jang, IS (1999). "Beta-glucuronidase-inhibitory activity and hepatoprotective effect of Ganoderma ...
Beta-glucuronidase can function through a wide range of pH values, and is fairly resistant to thermal inactivation. However, ... An organism is suitable for a GUS assay if it lacks naturally occurring β-glucuronidase activity or if the activity is very low ... There are different possible glucuronides that can be used as substrates for the β-glucuronidase, depending on the type of ... Jefferson, R. A.; Kavanagh, T. A.; Bevan, M. W. (1987). "GUS fusions: Beta-glucuronidase as a sensitive and versatile gene ...
Jefferson, R. A.; Kavanagh, T. A.; Bevan, M. W. (1987). "GUS fusions: Beta-glucuronidase as a sensitive and versatile gene ... "Beta-Glucuronidase from Escherichia Coli as a Gene-Fusion Marker". Proceedings of the National Academy of Sciences. 83 (22): ... including the role of glucuronidases, sulfatases and other enzymes in modulating and effecting enterohepatic circulation of ... sequencing and characterizing the first microbial glucuronidase, and creating transgenic technology for Caenorhabditis elegans ...
... ichthyosiform dermatosis and elevated serum beta-glucuronidase". Dermatologica. 177 (6): 341-7. doi:10.1159/000248604. PMID ...
The enzyme encoded by the gene UGT1A8 has glucuronidase activity with many substrates including anthraquinones.[14] ...
The C-terminal region of alpha-glucuronidase is mainly alpha-helical. It wraps around the catalytic domain, making additional ... Glycoside hydrolase family 67 includes alpha-glucuronidases, these are components of an ensemble of enzymes central to the ... Deletion mutants of alpha-glucuronidase from Bacillus stearothermophilus have indicated that the central region is responsible ... "The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A". Structure. 10 (4 ...
The disorder is caused by deficiency of the enzyme beta-glucuronidase. In its rarest form, Sly syndrome causes children to be ... is a recombinant human lysosomal beta glucuronidase for MPS VII (Sly syndrome) approved in the United States in November 2017 ( ...
It is a recombinant form of the human enzyme beta-glucuronidase. It was approved in the United States in November 2017, to ...
Baicalin-beta-D-glucuronidase (EC 3.2.1.167, baicalinase) is an enzyme with systematic name 5,6,7-trihydroxyflavone-7-O-beta-D- ... Baicalin-beta-D-glucuronidase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Zhang C, Zhang Y, Chen J, Liang X (2005). "Purification and characterization of baicalin-β-D-glucuronidase hydrolyzing baicalin ... Sasaki K, Taura F, Shoyama Y, Morimoto S (September 2000). "Molecular characterization of a novel beta-glucuronidase from ...
The KL gene encodes a type-I membrane protein that is related to β-glucuronidases. Reduced production of this protein has been ... The klotho protein is a novel β-glucuronidase (EC number 3.2.1.31) capable of hydrolyzing steroid β-glucuronides. Genetic ...
"Structure and Inhibition of Microbiome β-Glucuronidases Essential to the Alleviation of Cancer Drug Toxicity". Chemistry & ... gene to alleviate gastrointestinal cancer drug toxicity by observing structures and inhibition of the β-Glucuronidases micro- ...
Hess M, Stritzker J, Härtl B, Sturm JB, Gentschev I, Szalay AA (2011). "Bacterial glucuronidase as general marker for oncolytic ...
The enzyme encoded by this gene has moderate glucuronidase activity with phenols. [provided by RefSeq, Jul 2008]. GRCh38: ...
To do so, it produces a range of enzymes such as lyases, glucuronidases, sulfoesterases, and sulfamidases. The lyases have ...
Lu P, Boros S, Chang Q, Bindels RJ, Hoenderop JG (November 2008). "The beta-glucuronidase klotho exclusively activates the ... and Glucuronidase Klotho have been most extensively characterized [36, 37, 41, 42]. Functional consequences of TRPV6 channel ...
In the absence of sufficient bacteria, the bilirubin is de-conjugated by brush border β-glucuronidase and reabsorbed. This ...
Sly WS, Quinton BA, McAlister WH, Rimoin DL (1973). "Beta glucuronidase deficiency: report of clinical, radiologic, and ...
The enzyme encoded by the gene UGT1A8 has glucuronidase activity with many substrates including coumarins, anthraquinones and ...
Wilson KJ, Giller KE, Jefferson RA (1991), Hennecke H, Verma DP (eds.), "β-Glucuronidase (GUS) Operon Fusions as a Tool for ... Following on work done isolating numerous and diverse glucuronidases from microbial samples of African animal feces, and their ... required activation by skin-associated microbial glucuronidases and sulfatases. In the absence of microbial populations in the ... Jefferson described how the modulation of steroid and other hormone levels by microbial glucuronidases and arylsulfatase ...
Glucuronidase may refer to several enzymes: Alpha-glucuronidase Beta-glucuronidase Glycyrrhizinate beta-glucuronidase ... Glucuronosyl-disulfoglucosamine glucuronidase This set index page lists enzyme articles associated with the same name. If an ...
Alpha-glucuronidase Glucuronosyl-disulfoglucosamine glucuronidase Glycyrrhizinate beta-glucuronidase PDB: 1BHG​; Jain S, ... Human β-glucuronidase is a type of glucuronidase (a member of glycosidase Family 2) that catalyzes hydrolysis of β-D-glucuronic ... Human β-glucuronidase is located in the lysosome. In the gut, brush border β-glucuronidase converts conjugated bilirubin to the ... Glucuronidase at the US National Library of Medicine Medical Subject Headings (MeSH) Updated research on reporter glucuronidase ...
... rmewing at vax.oxford.ac.uk rmewing at vax.oxford.ac.uk Fri Aug 27 09:57:05 EST ... I am interested in obtaining an antibody to the Beta - glucuronidase enzyme (GUS) for use in maize tissue. Any offers / ...
Serum β-glucuronidase activity was assayed in 1134 patients with hepatobiliary or pancreatic diseases. 2. This test proved to ... Conversely, the presence of this disease is unlikely if the serum β-glucuronidase is normal. Consequently, the β-glucuronidase ... Premenopausal vaginal fluid β-glucuronidase values in relation to in situ cancer of the cervix.Cancer 7: 729, 1954.PubMedGoogle ... Fishman, W. H., Odell, L. D., Gill, J. E., andChristensen, R. A. The influence of stilbestrol on serum β-glucuronidase in women ...
... Joseph Walker jmwalke1 at FACSTAFF.WISC.EDU Thu Jul 13 07:41:41 EST 1995 *Previous ... If anyone knows of a supply of good anti B-glucuronidase antibodies please contact me at: jmwalke1 at facstaff.wisc.edu * ... I am looking for antibodies against B-glucuronidase to be used in an immunoprecipitation assay. We ran out of our lot and have ...
β-Glucuronidase from limpets (Patella vulgata) aqueous solution, 100,000 - 200,000 U/mL. ...
ß-glucuronidase and sulfatase activities in almost equal amounts, the preparation of ß-glucuronidase from E. coli is ... ß-Glucuronidase is a ~290 kDa tetrameric protein with an isoelectric point of 4.8.1 Unlike the enzyme preparations ... I>E. coli Source ß-Glucuronidases
... E. coli Source ß-Glucuronidases ß-Glucuronidase is a ~290 kDa ... ß-glucuronidase and sulfatase activities in almost equal amounts, the preparation of ß-glucuronidase from E. coli is ...
Furthermore, the pattern of glucuronidase gene expression in somatic hybrids qualitatively resembles that in livers of animal ...
Using a forward genetics approach, we identified the lysosomal enzyme β-glucuronidase (GUSB), a member of a large family of ...
... beta-glucuronidase activity, carbohydrate binding, identical protein binding, glucuronoside catabolic process ... beta-glucuronidase activity Source: EcoCyc ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for the ... Beta-glucuronidase (EC:3.2.1.31*Search proteins in UniProtKB for this EC number. ... "One step purification of Escherichia coli beta-glucuronidase.". Blanco C., Nemoz G.. Biochimie 69:157-161(1987) [PubMed] [ ...
Paradoxical Action of Solvents on Bacterial and Liver β-Glucuronidases. By MATTIE C. GAUTNEY, S. B. BARKER, S. RICHARDSON HILL ... Paradoxical Action of Solvents on Bacterial and Liver β-Glucuronidases. By MATTIE C. GAUTNEY, S. B. BARKER, S. RICHARDSON HILL ... Paradoxical Action of Solvents on Bacterial and Liver β-Glucuronidases Message Subject. (Your Name) has forwarded a page to you ... Dichloromethane and chloroform have been found to inhibit the action of liver β-glucuronidase on phenolphthalein glucuronic ...
Rabbit polyclonal Beta Glucuronidase (GUS) antibody validated for WB, IHC. Referenced in 2 publications. Immunogen ... Synthetic peptide corresponding to Escherichia coli Beta Glucuronidase (GUS) aa 1-16 (N terminal) conjugated to Keyhole Limpet ... Staining of the beta Glucuronidase band in immunoblotting is specifically inhibited by the immunizing peptide.. ...
Shop a large selection of products and learn more about Sigma Aldrich Fine Chemicals Biosciences BETA-GLUCURONIDASE FROM ESCHER ...
What is beta-glucuronidase? Meaning of beta-glucuronidase medical term. What does beta-glucuronidase mean? ... Looking for online definition of beta-glucuronidase in the Medical Dictionary? beta-glucuronidase explanation free. ... beta-glucuronidase. Also found in: Dictionary, Encyclopedia. β-glucuronidase. /β-glu·cu·ron·i·dase/ (gloo″ku-ron´ĭ-dās) an ... beta-glucuronidase. , β-glucuronidase (bāt′ă-gloo″kū-ron′ĭ-dās″) An enzyme found in lysosomes. It is involved in the breakdown ...
Here, we investigated if local expression of β-glucuronidase (βG) on cancer cells to catalytically convert SN38G to SN38 could ... Surface β-glucuronidase-expressing cells were 20 to 80-fold more sensitive to SN-38G than the parental cells. Intravenous CPT- ... Intestinal bacterial β-glucuronidase as a possible predictive biomarker of irinotecan-induced diarrhea severity *Ali N. ... The importance of tumor glucuronidase in the activation of irinotecan in a mouse xenograft model. J Pharmacol Exp Ther 2002; ...
Fripp, P. J. (‎1966)‎. Inapplicability of urinary β-glucuronidase determination as a field test of cure of urinary bilharziasis ... Inapplicability of urinary β-glucuronidase determination as a field test of cure of urinary bilharziasis. ...
... mice of the low β-glucuronidase strain, C3H/HeJ, showed no differences in hepatic levels of glycosaminoglycans (GAGs) when ... V. In vivo fate of erythrocyte-trapped β-glucuronidase in β-glucuronidase-deficient mice. Pediat. Res. 9918.Google Scholar ... Studies of β-glucuronidase, β-galactosidase, and N-acetyl-β-glucosaminidase in four tissues of the two types of mice at the two ... β-glucuronidase lysosomal enzymes mucopolysaccharidosis glycosaminoglycans mice This work was supported by NIH Clinical ...
Browse our beta-Glucuronidase/GUSB Peptides and Proteins all backed by our Guarantee+. ... beta-Glucuronidase/GUSB Peptides and Proteins available through Novus Biologicals. ... beta-Glucuronidase/GUSB Proteins. We offer beta-Glucuronidase/GUSB Peptides and beta-Glucuronidase/GUSB Proteins for use in ... beta-Glucuronidase/GUSB protein, GUSB protein, beta-D-glucuronidase protein, beta-G1 protein, beta-glucuronidase protein, BG ...
The hydrolysis of glucuronides can be catalysed by beta-glucuronidase, an enzyme expressed in many tissues and body fluids in ... The possible contribution of beta-glucuronidase to drug disposition in humans has not been assessed i … ... The role of beta-glucuronidase in drug disposition and drug targeting in humans Clin Pharmacokinet. 1997 Jul;33(1):18-31. doi: ... Moreover, as beta-glucuronidase has already been proven to be useful in tumour specific bioactivation of glucuronide prodrugs ...
Mouse Monoclonal Anti-beta-Glucuronidase/GUSB Antibody (105). Validated: WB, ELISA, IHC, IHC-Fr. Tested Reactivity: Human. 100 ... Additional beta-Glucuronidase/GUSB Products. beta-Glucuronidase/GUSB NBP1-42247 * beta-Glucuronidase/GUSB Antibodies ... Home » beta-Glucuronidase/GUSB » beta-Glucuronidase/GUSB Antibodies » beta-Glucuronidase/GUSB Antibody (105) ... Blogs on beta-Glucuronidase/GUSB. There are no specific blogs for beta-Glucuronidase/GUSB, but you can read our latest blog ...
We have characterized a new mutant mouse that has virtually no beta-glucuronidase activity. This biochemical defect causes a ... Characterization of a mouse with beta-glucuronidase deficiency J Clin Invest. 1989 Apr;83(4):1258-66. doi: 10.1172/JCI114010. ... We have characterized a new mutant mouse that has virtually no beta-glucuronidase activity. This biochemical defect causes a ... beta-glucuronidase deficiency). Genetic analysis showed that the mutation is inherited as an autosomal recessive that maps to ...
At pH 4, the Km ranged 44-fold from 22 µM for quercetin-4-glucuronide with Helix pomatia ß-glucuronidase, to 981 µM for para- ... Mammalian ß-glucuronidase released from neutrophils at inflammatory sites may be able to deconjugate and thus activate ... In conclusion, we show that ß-glucuronidase from four different sources, including human neutrophils, is able to deconjugate ... We have studied deconjugation kinetics and pH optimum for four sources of ß-glucuronidase (human neutrophil, human recombinant ...
Metabolism of quercetin-7- and quercetin-3-glucuronides by an in vitro hepatic model: the role of human beta-glucuronidase, ... These results show that HepG2 cells can absorb and turnover quercetin glucuronides and that human endogenous beta-glucuronidase ... hydrolysis of the glucuronide by endogenous beta-glucuronidase followed by sulfation to quercetin-3-sulfate (7% of quercetin-7 ... and quercetin-3-glucuronide to the mono-sulfate conjugate shows intracellular deglucuronidation by beta-glucuronidase activity ...
Beta-d-glucuronidase (Science: enzyme) An enzyme catalyzing the hydrolysis of various beta-d-glucuronides, liberating free d- ... Retrieved from "https://www.biology-online.org/dictionary/index.php?title=Beta-d-glucuronidase&oldid=66563" ...
X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely ... For researchers wishing to investigate the suitability of our various chromogenic β-glucuronidase substrates, we offer this ...
Glucuronidase Complexed with Its Substrate and Products: MECHANISTIC IMPLICATIONS. ... alpha-D-glucuronidase A 679 Geobacillus stearothermophilus EC#: 3.2.1.131 IUBMB Gene Name(s): aguA ... The 1.7 A crystal structure of alpha-D-glucuronidase, a family-67 glycoside hydrolase from Bacillus stearothermophilus T-1. * ...
Glucuronidase Complexed with Its Substrate and Products: MECHANISTIC IMPLICATIONS. ... Description: alpha-D-glucuronidase protein , Length: 679 No structure alignment results are available for 1K9E.A explicitly.. ... Crystal structure of a mutated family-67 alpha-D-glucuronidase (E285N) from Bacillus stearothermophilus T-6, complexed with 4-O ...
Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from ... Major virulence genes are shared between typical (β-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ... The uidA gene, which encodes β-glucuronidase, was intact in all the GP STEC O157:H7 strains sequenced in this study, as well as ... General genomic features of the β-glucuronidase-positive STEC O157:H7 strain PV15-279 from Japan and the typical STEC O157:H7 ...
Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from ... Major virulence genes are shared between typical (β-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ... Genomic Characterization of β-Glucuronidase-Positive Escherichia coli O157:H7 Producing Stx2a Yoshitoshi Ogura. , Kazuko Seto, ... Isolation and characterization of a beta-D-glucuronidase-producing strain of Escherichia coli serotype O157:H7 in the United ...
Analysis of enzymes, such as β-D-glucuronidase in indicator bacteria have been used for many years to quantify feces-associated ... In this work we are introducing a novel parameter: enzymatic activity of beta-D-glucuronidase expressed in Modified Fishman ... E. coli, fecal contamination, water monitoring, enzymatic activity, glucuronidase, modified fishman units, MFU/100ml, ...
  • Using a forward genetics approach, we identified the lysosomal enzyme β-glucuronidase (GUSB), a member of a large family of coregulated lysosomal enzymes, as a key regulator of Lyme-associated arthritis severity. (jci.org)
  • Because children with the disease lack a functional form of an enzyme called beta-glucuronidase (GUSB), Sands and associates tried to prevent the disorder by using AAV as a viral vector to deliver the GUSB to the mice. (thefreedictionary.com)
  • We offer beta-Glucuronidase/GUSB Peptides and beta-Glucuronidase/GUSB Proteins for use in common research applications: Blocking/Neutralizing, Control, ELISA, Protein Array, Western Blot. (novusbio.com)
  • Each beta-Glucuronidase/GUSB Peptide and beta-Glucuronidase/GUSB Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Our beta-Glucuronidase/GUSB Peptides and beta-Glucuronidase/GUSB Proteins can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our beta-Glucuronidase/GUSB Peptides and Proteins. (novusbio.com)
  • There are currently no images for beta-Glucuronidase/GUSB Antibody (NBP1-42247). (novusbio.com)
  • The GUSB gene encodes beta-glucuronidase (EC 3.2.1.31), a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al. (novusbio.com)
  • GUSB / Beta Glucuronidase is a hydrolase that degrades glycosaminoglycans, including heparan sulfate, dermatan sulfate, and chondroitin-4,6-sulfate. (lsbio.com)
  • Rabbit IgG polyclonal antibody for Beta-glucuronidase(GUSB) detection. (bosterbio.com)
  • A synthetic peptide corresponding to a sequence at the N-terminus of human beta glucuronidase(GUSB)(50-70aa DFSDNRRRGFEEQWYRRPLWE). (bosterbio.com)
  • GUSB(Beta-Glucuronidase) is an enzyme that in humans is encoded by the GUSB gene. (bosterbio.com)
  • GUSB (Glucuronidase Beta) is a Protein Coding gene. (genecards.org)
  • Human β-glucuronidase is homologous to the Escherichia coli enzyme β-galactosidase. (wikipedia.org)
  • E. coli Source ß-Glucuronidases ß-Glucuronidase is a ~290 kDa tetrameric protein with an isoelectric point of 4.8. (sigmaaldrich.com)
  • 1 Unlike the enzyme preparations from mollusks that naturally contain ß-glucuronidase and sulfatase activities in almost equal amounts, the preparation of ß-glucuronidase from E. coli is essentially free of sulfatase activity. (sigmaaldrich.com)
  • Synthetic peptide corresponding to Escherichia coli Beta Glucuronidase (GUS) aa 1-16 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). (abcam.com)
  • X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely used for monitoring E. coli contamination in food (such as meat, dairy products and shellfish, etc) and water, and for the detection of GUS gene expression in plants. (biotium.com)
  • Hayes PS , Blom K , Feng P , Lewis J , Strockbine NA , Swaminathan B . Isolation and characterization of a beta-D-glucuronidase-producing strain of Escherichia coli serotype O157:H7 in the United States. (cdc.gov)
  • One of them was biochemically typical of E. coli O157:H7, that is, neither fermented sorbitol nor exhibited β-glucuronidase (GUD) activity, and carried genes encoding Shiga toxins (Stx) 1 and 2, the intimin subtype γ1, the enterohemorrhagic E. coli (EHEC)-hemolysin, and the ter gene cluster. (unboundmedicine.com)
  • A GH67 α-glucuronidase gene derived from Bacillus halodurans C-125 was expressed in E. coli to obtain a recombinant enzyme ( Bh GlcA67). (go.jp)
  • This is the first report of a β-glucuronidase gene cloned from a bacterial source other than Escherichia coli . (asm.org)
  • Bacterial β-glucuronidase activity has been considered for many years to be almost unique to Escherichia coli and closely related Enterobacteriaceae ( 53 ). (asm.org)
  • Despite reports of enzyme activity associated with other bacteria and the physiological importance of β-glucuronidase to human health, only the genetic elements for the E. coli enzyme have been identified and studied ( 26 , 53 ). (asm.org)
  • Efficacy of beta-glucuronidase assay for identification of Escherichia coli by the defined-substrate technology. (asm.org)
  • All subjects carried beta-glucuronidase-positive E. coli. (asm.org)
  • This part of ISO 16649 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli in products intended for human consumption or for the feeding of animals. (complianceonline.com)
  • β-D-Galactosidase and β-D-glucuronidase are 2 marker enzymes used in the rapid detection of total coliforms and E. coli , respectively. (scielo.org.za)
  • A study of the activity of Escherichia coli -D-glucuronidase (GUS) in polluted stagnant and running water samples was performed with an objective of assessing the viability of a direct marker enzyme assay as a suitable alternative to membrane filtration for the indication of faecal pollution in water intended for drinking purposes. (ajol.info)
  • Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli. (bsigroup.com)
  • Méthode horizontale pour le dénombrement des Escherichia coli bêta-glucuronidase positive. (bsigroup.com)
  • Horizontales Verfahren zur Zählung von ß-Glucuronidase-positiven Escherichia coli. (bsigroup.com)
  • High purity recombinant β-Glucuronidase ( Escherichia coli ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • A medium containing a chromogenic substrate was developed for the enumeration of Escherichia coli on the basis of beta-glucuronidase activity. (surrey.ac.uk)
  • This relationship applied even when the E. coli population contained 5% beta-glucuronidase-negative cells. (surrey.ac.uk)
  • It is used to detect E. coli contamination in food and drinking water due to their β-glucuronidase activity. (p212121.com)
  • The human b-Glucuronidase is found in lysosomes and is homologe to the Gene from E.coli (uidA) which is used as reporter. (pjk-gmbh.de)
  • (2) Using this test system, glucuronidase-positive, indole-positive, gram-negative organisms are presumptively identified as E. coli . (hardydiagnostics.com)
  • According to Thompson, approximately 96% of all E. coli strains are glucuronidase-positive. (hardydiagnostics.com)
  • Presumptive identification of E. coli is indicated when both glucuronidase and indole tests are positive and the organism is gram-negative. (hardydiagnostics.com)
  • If a colony of typical morphology has been determined to be gram-negative, both glucuronidase and indole must be positive in order to presumptively identify it as E. coli . (hardydiagnostics.com)
  • Although, glucuronidase- and indole-positive, some atypical strains of E. coli ( E. coli group AD) are non-lactose-fermenters. (hardydiagnostics.com)
  • E. coli O157 appear as lactose-fermenters but are glucuronidase-negative and indole-positive. (hardydiagnostics.com)
  • The anti-pan carcinoma monoclonal antibody (MAb) 323/A3, linked to E. coli-derived β-glucuronidase (GUS) was used to study the tumour-site-selective activation of the prodrug Epirubicin-glucuronide (Epi-glu). (elsevier.com)
  • Fo4 was transformed by GUS gene fusion system (Escherichia coli β-D-glucuronidase gene) and GFP (Green Fluorescent Proteins). (upm.edu.my)
  • Concentration of Escherichia coli can be quantified based on a digestive enzyme produced by 97% of E. coli strains called β-glucuronidase (β-GUS). (macewan.ca)
  • Using analysis of labeled β-glucuronidase peptides after hydrolysis of a substrate that enters a very stable intermediate stage, researchers have determined that Glu540 is the nucleophilic residue. (wikipedia.org)
  • A study was undertaken to determine the efficacy and specificity of the defined-substrate technology beta-glucuronidase (Colilert) assay for the identification of this species from fecal samples. (asm.org)
  • PNPG (Gus) is a chromogenic β-glucuronidase substrate yielding a yellow solution upon cleavage. (p212121.com)
  • I am interested in obtaining an antibody to the Beta - glucuronidase enzyme (GUS) for use in maize tissue. (bio.net)
  • The antibody reacts with the Beta-Glucuronidase antigen. (novusbio.com)
  • This antibody reacts with an non-active center epitope on human placental and liver beta-Glucuronidase. (novusbio.com)
  • This antibody is useful for staining of human Beta-Glucuronidase in ELISA, Western blotting and frozen sections. (novusbio.com)
  • The antibody can also be used for affinity purification of human Beta-Glucuronidase. (novusbio.com)
  • Pinedo, H. M. / A monoclonal antibody-β-glucuronidase conjugate as activator of the prodrug epirubicin-glucuronide for specific treatment of cancer . (elsevier.com)
  • Human β-glucuronidase is a type of glucuronidase (a member of glycosidase Family 2) that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans) such as heparan sulfate. (wikipedia.org)
  • The hydrolysis of glucuronides can be catalysed by beta-glucuronidase, an enzyme expressed in many tissues and body fluids in humans. (nih.gov)
  • Therefore, in commercial enzyme cocktails α-glucuronidases are crucial in addition to endo-xylanases and β-xylosidases, to achieve a complete hydrolysis of monosaccharides. (biomedcentral.com)
  • Chemical method for the determination of 17-hydroxycorticosteroids and 17-ketosteroids in urine following hydrolysis with beta-glucuronidase. (semanticscholar.org)
  • CovaChem's β-Glucuronidase is prepared to provide optimal hydrolysis of Glucuroconjugates in urine samples. (covachem.com)
  • Hydrolysis of the additive nitrophenyl-beta-glucopyranosiduronic acid denotes the production of glucuronidase and is detected by the development of a yellow color in the medium. (hardydiagnostics.com)
  • Glucuronidase may refer to several enzymes: Alpha-glucuronidase Beta-glucuronidase Glycyrrhizinate beta-glucuronidase Glucuronosyl-disulfoglucosamine glucuronidase This set index page lists enzyme articles associated with the same name. (wikipedia.org)
  • Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. (wikipedia.org)
  • Analysis of enzymes, such as β-D-glucuronidase in indicator bacteria have been used for many years to quantify feces-associated contamination, but the measurement parameter which allows the comparison of the obtained results is not clearly defined. (witpress.com)
  • In this study, the effect of 11 US EPA priority pollutant phenols (PPP) on these enzyme assays were investigated and it was shown that over- and under-estimation of β-D-galactosidase and β-D-glucuronidase activities may occur due to inhibition or activation of these enzymes in the presence of these phenolic compounds. (scielo.org.za)
  • The characterisation of α-glucuronidases is a necessity to find the right enzymes to improve degradation of recalcitrant UA me xylan structures. (biomedcentral.com)
  • It contains a mixture of enzymes including ß-glucuronidase and sulfatase. (perkinelmer.co.uk)
  • β-galactosidase and β-glucuronidase enzymes are commonly used as reporters for gene expression from gene promoter-lacZ or uidA fusions (respectively). (edu.au)
  • Taryn L. O'Loughlin and Ichiro Matsumura, " HIV Protease-Activated Molecular Switches Based on Beta-Glucuronidase and Alkaline Phosphatase", Combinatorial Chemistry & High Throughput Screening (2006) 9: 313. (eurekaselect.com)
  • It is a lysosomal storage disorder caused by deficiency of an enzyme called beta-glucuronidase , which causes an abnormal buildup of toxic materials in the body's cells. (thefreedictionary.com)
  • Variation in the phenotypic expression of β-glucuronidase deficiency. (springer.com)
  • Mucopolysaccharidosis VII: β-Glucuronidase deficiency. (springer.com)
  • Beta glucuronidase deficiency: Report of clinical radiologic, and biochemical features of a new mucopolysaccharidosis. (springer.com)
  • beta-glucuronidase deficiency). (nih.gov)
  • MPS VII is caused by the deficiency of beta-glucuronidase, an enzyme required for the breakdown of the glycosaminoglycans (GAGs) dermatan sulfate, chondroitin sulfate and heparan sulfate. (salesandmarketingnetwork.com)
  • Human beta-glucuronidase deficiency mucopolysaccharidosis: ide. (mysciencework.com)
  • Human beta-glucuronidase deficiency mucopolysaccharidosis: identification of cross-reactive antigen in cultured fibroblasts of deficient patients by enzyme immunoassay. (mysciencework.com)
  • An enzyme immunoassay for human beta-glucuronidase was developed to determine the presence or absence of antigenically cross-reactive material (CRM) in patients with beta-glucuronidase deficiency mucopolysaccharidosis. (mysciencework.com)
  • Feline mucopolysaccharidosis VII due to beta-glucuronidase deficiency. (unil.ch)
  • Enzyme potentiated hyposensitization II: Effect of glucose, glucosamine, N-acetylaminosugars and gelatin on the ability of beta-glucuronidase to block the anamnestic response to antigen in mice. (thefreedictionary.com)
  • Among other types there appeared to be some inverse relation between production of glucuronidase and of the M antigen. (microbiologyresearch.org)
  • 4. The serum β-glucuronidase test is relatively nonspecific, since elevations were observed in a variety of diseases affecting the liver as well as during pregnancy and after the administration of estrogens. (springer.com)
  • Dichloromethane and chloroform have been found to inhibit the action of liver β-glucuronidase on phenolphthalein glucuronic acid at concentrations which produce a considerable enhancement of bacterial β-glucuronidase. (sciencemag.org)
  • Liver-glucuronidase activity of inbred mouse strains. (springer.com)
  • Goat antiserum to partially purified human placenta beta-glucuronidase did not recognize differences in normal enzyme from human placenta, liver, fibroblasts, or blood platelets. (mysciencework.com)
  • The sugar chains of microsomal and lysosomal β-glucuronidases of rat liver were studied by endo-β-N-acetylglucosaminidase H digestion and by hydrazinolysis. (elsevier.com)
  • High purity recombinant α-Glucuronidase ( Geobacillus stearothermophilus ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • 2013) or 4-Methylumbelliferyl β-D-glucuronide (for β-glucuronidase assays) (Ramsay et al. (edu.au)
  • Through comparison to the structural data of the homologous enzyme xylanase, it has been suggested that Tyr504 of β-glucuronidase might stabilize the leaving nucleophile (Glu540) or modulate its activity. (wikipedia.org)
  • More recently researchers have discovered a feline family that exhibits deficiencies in β-glucuronidase activity. (wikipedia.org)
  • 1. Serum β-glucuronidase activity was assayed in 1134 patients with hepatobiliary or pancreatic diseases. (springer.com)
  • 3. Increased serum β-glucuronidase activity was as sensitive as index of hepatic dysfunction in patients with acute hepatitis or drug-induced jaundice as the serum glutamic oxalacetic transaminase test and was more sensitive than the other hepatic tests commonly employed in the confirmation of these diseases. (springer.com)
  • 5. The pathophysiologic alterations underlying variations of serum β-glucuronidase activity in hepatobiliary disease are discussed. (springer.com)
  • Rutenburg, S. H. , and Seligman, A. M. Colorimetric estimation of β-D-glucuronidase activity with 8-benzoylamino-2-naphthyl β-D-glucuronide. (springer.com)
  • V. The menstrual cycle and serum β-glucuronidase activity in estrogen treated postmenopausal subjects. (springer.com)
  • Cohen, S. L. , and Huseby, R. A. The effect of estrogen on serum glucuronidase activity of patients with breast cancer. (springer.com)
  • Both groups of strains showed higher enzymatic activity for alpha-galactosidase, alpha-glucosidase, beta-glucuronidase , beta-glucosidase, chymotrypsin, and naphthyl phosphohydrolase. (thefreedictionary.com)
  • lysozyme, beta-glucuronidase and N-acetyl beta-glucosaminidase in adult leprosy correlating the levels with activity of the disease. (thefreedictionary.com)
  • 1 nmol), and the lowest activity of beta-glucuronidase (an average of 0. (thefreedictionary.com)
  • Here, we investigated if local expression of β-glucuronidase (βG) on cancer cells to catalytically convert SN38G to SN38 could enhance the antitumor activity of CPT-11. (nature.com)
  • Studies of β-glucuronidase, β-galactosidase, and N -acetyl-β-glucosaminidase in four tissues of the two types of mice at the two ages revealed that glucuronidase was the only enzyme with lower activity in the C3H/HeJ strain. (springer.com)
  • We have characterized a new mutant mouse that has virtually no beta-glucuronidase activity. (nih.gov)
  • The conversion of the quercetin-7- and quercetin-3-glucuronide to the mono-sulfate conjugate shows intracellular deglucuronidation by beta-glucuronidase activity, allowing transient contact of the free aglycone with the cellular environment. (nih.gov)
  • These results show that HepG2 cells can absorb and turnover quercetin glucuronides and that human endogenous beta-glucuronidase activity could modulate the intracellular biological activities of dietary antioxidant flavonoids. (nih.gov)
  • In this work we are introducing a novel parameter: enzymatic activity of beta-D-glucuronidase expressed in Modified Fishman Units per 100 ml of water sample (MFU/100ml). (witpress.com)
  • These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. (openwetware.org)
  • Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols . (openwetware.org)
  • Two overlapping clones that restored β-glucuronidase activity in the mutant strain were sequenced and revealed three complete and two partial open reading frames. (asm.org)
  • An analysis of other lactobacilli identified β-glucuronidase activity and gusA homologs in other L. gasseri isolates but not in other Lactobacillus species tested. (asm.org)
  • However, reduction of these β- d -glucuronides in the GI tract by bacterial β-glucuronidase activity frees the aglycone components, allowing them to reenter the bloodstream. (asm.org)
  • Additionally, bacterial β-glucuronidase activity has been implicated in the generation of toxic and carcinogenic metabolites which may be important precursors to tumor initiation and large bowel cancer in the gut ( 6 , 36 ). (asm.org)
  • However, evidence has slowly been accumulating to indicate that β-glucuronidase activity can also be found in a limited number of other bacteria, particularly gram-positive inhabitants of the GI tract ( 3 , 4 , 23 , 36 ). (asm.org)
  • Studies with various Lactobacillus species, including L. gasseri , have consistently shown their ability to reduce the amount of fecal β-glucuronidase activity and lower the occurrence of cancer indicators present in the GI tract ( 15 , 27 , 33 , 37 , 41 ). (asm.org)
  • The 2767-bp 5[prime] upstream sequence of the tpi gene was fused translationally with the [beta]-glucuronidase (gusA) gene, and the resulting construct, TPI-GUS, was found to express constitutive, high levels of GUS activity in transgenic tobacco (Nicotiana tabacum) plants. (plantphysiol.org)
  • The purpose of this study was to investigate the effect of heat treatment on the β-glucuronidase inhibition activity of DOPA. (go.jp)
  • AIM Beta-glucuronidase activity is increased in the cerebrospinal fluid (CSF) of patients with bacterial meningitis. (semanticscholar.org)
  • The aim of this study was to investigate the beta-glucuronidase activity in the cell-free CSF of bacterial meningitis and its course during treatment, and compare it with other CSF parameters. (semanticscholar.org)
  • One Unit of α-D-glucuronidase activity is defined as the amount of enzyme required to release one µmole of α-D-glucuronic acid per minute from aldouronic acid in MOPS buffer (100 mM), pH 7.0 at 70 o C. (megazyme.com)
  • Beta-glucuronidase-inhibitory activity and hepatoprotective effect of Ganoderma lucidum . (medchemexpress.com)
  • that the urine from rats on the basic diet contained an inhibitor of beta-glucuronidase activity in a quantity greater than that from sucrose-fed animals. (medicalnewstoday.com)
  • During transit of the glucuronidated product through the gastrointestinal tract, an induced upregulation of gut microbial β-glucuronidase (GUS) activity may cause severe diarrhea and thus force many patients to stop treatment. (cdc.gov)
  • To demonstrate the functional effects of fecal enzymatic activity, we examined β-glucuronidase-mediated cleavage of serotonin β-D-glucuronide (5-HT-GLU) and the resultant production of free 5-HT by HPLC. (ucc.ie)
  • As expected, β-glucuronidase and β-glucosidase activity were absent in germ-free mice. (ucc.ie)
  • β-glucuronidase and β-glucosidase activity remained at reduced levels for nearly two weeks after cessation of antibiotic administration. (ucc.ie)
  • Dietary targeting of the microbiota using prebiotics did not alter β-glucuronidase or β-glucosidase activity. (ucc.ie)
  • In conclusion, we show that ß-glucuronidase from four different sources, including human neutrophils, is able to deconjugate flavonoid glucuronides and non-flavonoid substrates at fairly similar kinetic rates. (wur.nl)
  • For researchers wishing to investigate the suitability of our various chromogenic β-glucuronidase substrates, we offer this sampler kit, which contains 5 mg each of the following three different substrates: X-GlcU, CHA ( 10016 ), Red-β-D-GlcU, CHA ( 10018 ), and Rose-β-D-GlcU, CHA ( 10019 ). (biotium.com)
  • Consequently, the β-glucuronidase assay is a valuable screening test for this disease. (springer.com)
  • I am looking for antibodies against B-glucuronidase to be used in an immunoprecipitation assay. (bio.net)
  • In 1976, Kilian and Bulow described the association of beta-glucuronidase with the genus Escherichia (97% positive) and suggested that a beta-glucuronidase assay would be a useful identification test. (asm.org)
  • The environment for recognizing the 4- O -methyl group of glucuronic acid was observed in all the crystal structures of reported GH67 glucuronidases, and the amino acids for discriminating the 4- O -methyl group of glucuronic acid were widely conserved in the primary sequences of the GH67 family, suggesting that the 4- O -methyl group is critical for the activities of the GH67 family. (go.jp)
  • SUMMARY: Seven hundred and thirty-six strains of Streptococcus pyogenes were examined for production of glucuronidase. (microbiologyresearch.org)
  • ABBOO, Sagaran y PLETSCHKE, Brett I . Effect of phenolic compounds on the rapid direct enzymatic detection of β -D-galactosidase and β -D-glucuronidase . (scielo.org.za)
  • The action of bacterial β-glucuronidase in the gastrointestinal (GI) tract is an important component in the enterohepatic circulation of many hydrophobic xenobiotics and endogenous waste compounds. (asm.org)
  • This study aimed to develop a mathematical model for the extraction of Pyrus elaeagrifolia Pallas leaves using the Box-Behnken design, to investigate bioactivity and bacterial β-glucuronidase (GUS) inhibitory potential of the extracts obtained under optimum conditions. (springer.com)
  • Beta-glucuronidase in the diagnosis of bacterial meningitis and response to treatment. (semanticscholar.org)
  • Non-sorbitol-fermenting (NSF) and β-glucuronidase-negative (GN) STEC O157:H7 (hereafter referred to as typical STEC O157:H7), the major clone among currently circulating STEC O157:H7 strains, frequently causes large outbreaks of severe enteric infections, including diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome. (cdc.gov)
  • We performed a kinetic study on β-glucuronidase inhibition by hDOPA using Lineweaver-Burk analysis. (go.jp)
  • Results showed 95.5% beta-glucuronidase-positive isolates in 24 h and 99.5% positive after 28 h of incubation. (asm.org)
  • It uses a colony-count technique after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase. (complianceonline.com)
  • Incubation of human beta-glucuronidase with 2-deoxy-2-fluoro-beta-D-glucuronyl fluoride resulted in time-dependent inactivation of the enzyme through the accumulation of a covalent 2-deoxy-2-fluoro-alpha-D-glucuronyl-enzyme, as observed by electrospray mass spectrometry. (ubc.ca)
  • CRM was identified in fibroblast extracts from all four of the unrelated beta-glucuronidase-deficient patients studied, but titration patterns indicated genetic heterogeneity among these four mutant proteins. (mysciencework.com)
  • Glucuronidase enzyme or other proteins. (pjk-gmbh.de)
  • Moreover, as beta-glucuronidase has already been proven to be useful in tumour specific bioactivation of glucuronide prodrugs of anticancer agents, we also focus on anticancer prodrug approaches utilising beta-glucuronidase. (nih.gov)
  • The possible contribution of beta-glucuronidase to drug disposition in humans has not been assessed in a systematic manner, but this enzyme may be able to release, locally or systemically, the active or inactive parent compound from drug glucuronides, thereby modifying the disposition and action of these drugs. (nih.gov)
  • Since some issues surrounding the beta-glucuronidase-mediated deconjugation of drug glucuronides still need to be clarified in humans, additional data from animal models supporting this concept have been included. (nih.gov)
  • Mammalian ß-glucuronidase released from neutrophils at inflammatory sites may be able to deconjugate and thus activate flavonoid glucuronides. (wur.nl)
  • We have studied deconjugation kinetics and pH optimum for four sources of ß-glucuronidase (human neutrophil, human recombinant, myeloid PLB-985 cells, Helix pomatia) with five flavonoid glucuronides (quercetin-3-glucuronide, quercetin-3'-glucuronide, quercetin-4'-glucuronide, quercetin-7-glucuronide, 3'-methylquercetin-3-glucuronide), 4-methylumbelliferyl-ß-D-glucuronide, and para-nitrophenol-glucuronide. (wur.nl)
  • Metabolism of quercetin-7- and quercetin-3-glucuronides by an in vitro hepatic model: the role of human beta-glucuronidase, sulfotransferase, catec. (nih.gov)
  • Human β-glucuronidase is located in the lysosome. (wikipedia.org)
  • Human β-glucuronidase is synthesized as an 80 kDa monomer (653 amino acids) before proteolysis removes 18 amino acids from the C-terminal end to form a 78 kDa monomer. (wikipedia.org)
  • There were no significant differences in the percentage of beta-glucuronidase-positive isolates among the human or animal isolates. (asm.org)
  • Human beta-glucuronidase is a member of the Family 2 glycosylhydrolases that cleaves beta-D-glucuronic acid residues from the nonreducing termini of glycosaminoglycans. (ubc.ca)
  • We conclude that despite certain limitations, the enzyme immunoassay for human beta-glucuronidase is useful and that all four beta-glucuronidase-deficient patients studied possess CRM. (mysciencework.com)
  • Random Elongation Mutagenesis [1] was used to fuse the p6 peptide, which is recognized and cleaved by HIV protease, and twelve random sequence amino acids to the C-termini of beta-glucuronidase (GUS) and alkaline phosphatase (AP). (eurekaselect.com)
  • The gusA gene, encoding a new β-glucuronidase enzyme, has been cloned from Lactobacillus gasseri ADH. (asm.org)
  • Overexpression of GusA was achieved in a β-glucuronidase-negative L. gasseri strain by expressing the gusA gene, subcloned onto a low-copy-number shuttle vector, from the strong Lactobacillus P6 promoter. (asm.org)
  • Genetic analysis showed that the mutation is inherited as an autosomal recessive that maps to the beta-glucuronidase gene complex, [Gus], on the distal end of chromosome 5. (nih.gov)
  • The properties of genetic variants of beta-glucuronidase falling into each of these classes are described. (jax.org)
  • Genetic chimeras were induced in embryos doubly heterozygous for a heat shock regulated Cre recombinase and a constitutively expressed beta-glucuronidase (GUS) gene flanked by the loxP binding sites for Cre. (nih.gov)
  • The considerable overlap of values in obstructive and primary hepatic jaundice renders the β-glucuronidase test of limited value in the differential diagnoisis of jaundice. (springer.com)
  • Young (60-80 days) mice of the low β-glucuronidase strain, C3H/HeJ, showed no differences in hepatic levels of glycosaminoglycans (GAGs) when compared to the randombred, "normal" Swiss-Webster mice of the same age. (springer.com)