An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.
An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
A flavoprotein enzyme that catalyzes the univalent reduction of OXYGEN using NADPH as an electron donor to create SUPEROXIDE ANION. The enzyme is dependent on a variety of CYTOCHROMES. Defects in the production of superoxide ions by enzymes such as NADPH oxidase result in GRANULOMATOUS DISEASE, CHRONIC.
Glucose in blood.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
A glucose dehydrogenase that catalyzes the oxidation of beta-D-glucose to form D-glucono-1,5-lactone, using NAD as well as NADP as a coenzyme.
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
An iron-molybdenum flavoprotein containing FLAVIN-ADENINE DINUCLEOTIDE that oxidizes hypoxanthine, some other purines and pterins, and aldehydes. Deficiency of the enzyme, an autosomal recessive trait, causes xanthinuria.
An enzyme derived from cow's milk. It catalyzes the radioiodination of tyrosine and its derivatives and of peptides containing tyrosine.
Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.
Method of analyzing chemicals using automation.
D-Glucose:1-oxidoreductases. Catalyzes the oxidation of D-glucose to D-glucono-gamma-lactone and reduced acceptor. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.47; EC 1.1.1.118; EC 1.1.1.119 and EC 1.1.99.10.
A test to determine the ability of an individual to maintain HOMEOSTASIS of BLOOD GLUCOSE. It includes measuring blood glucose levels in a fasting state, and at prescribed intervals before and after oral glucose intake (75 or 100 g) or intravenous infusion (0.5 g/kg).
A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.
An enzyme that catalyzes the oxidative deamination of naturally occurring monoamines. It is a flavin-containing enzyme that is localized in mitochondrial membranes, whether in nerve terminals, the liver, or other organs. Monoamine oxidase is important in regulating the metabolic degradation of catecholamines and serotonin in neural or target tissues. Hepatic monoamine oxidase has a crucial defensive role in inactivating circulating monoamines or those, such as tyramine, that originate in the gut and are absorbed into the portal circulation. (From Goodman and Gilman's, The Pharmacological Basis of Therapeutics, 8th ed, p415) EC 1.4.3.4.
The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.
An enzyme oxidizing peptidyl-lysyl-peptide in the presence of water & molecular oxygen to yield peptidyl-allysyl-peptide plus ammonia & hydrogen peroxide. EC 1.4.3.13.
An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A pathological state in which BLOOD GLUCOSE level is less than approximately 140 mg/100 ml of PLASMA at fasting, and above approximately 200 mg/100 ml plasma at 30-, 60-, or 90-minute during a GLUCOSE TOLERANCE TEST. This condition is seen frequently in DIABETES MELLITUS, but also occurs with other diseases and MALNUTRITION.
An aldehyde oxidoreductase expressed predominantly in the LIVER; LUNGS; and KIDNEY. It catalyzes the oxidation of a variety of organic aldehydes and N-heterocyclic compounds to CARBOXYLIC ACIDS, and also oxidizes quinoline and pyridine derivatives. The enzyme utilizes molybdenum cofactor and FAD as cofactors.
An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used.
The rate dynamics in chemical or physical systems.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Narrow pieces of material impregnated or covered with a substance used to produce a chemical reaction. The strips are used in detecting, measuring, producing, etc., other substances. (From Dorland, 28th ed)
A condensation product of riboflavin and adenosine diphosphate. The coenzyme of various aerobic dehydrogenases, e.g., D-amino acid oxidase and L-amino acid oxidase. (Lehninger, Principles of Biochemistry, 1982, p972)
The appearance of an abnormally large amount of GLUCOSE in the urine, such as more than 500 mg/day in adults. It can be due to HYPERGLYCEMIA or genetic defects in renal reabsorption (RENAL GLYCOSURIA).
An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
Inorganic salts of the hypothetical acid ferrocyanic acid (H4Fe(CN)6).
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
An enzyme that catalyzes the conversion of ATP and a D-hexose to ADP and a D-hexose 6-phosphate. D-Glucose, D-mannose, D-fructose, sorbitol, and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase. (From Enzyme Nomenclature, 1992) EC 2.7.1.1.
A ubiquitously expressed glucose transporter that is important for constitutive, basal GLUCOSE transport. It is predominately expressed in ENDOTHELIAL CELLS and ERYTHROCYTES at the BLOOD-BRAIN BARRIER and is responsible for GLUCOSE entry into the BRAIN.
Platinum. A heavy, soft, whitish metal, resembling tin, atomic number 78, atomic weight 195.09, symbol Pt. (From Dorland, 28th ed) It is used in manufacturing equipment for laboratory and industrial use. It occurs as a black powder (platinum black) and as a spongy substance (spongy platinum) and may have been known in Pliny's time as "alutiae".
A generic term for a variety of compounds that contain silicon, oxygen, and magnesium, and may contain hydrogen. Examples include TALC and some kinds of ASBESTOS.

Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals. (1/420)

Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a reporter system based on the Aspergillus niger glucose oxidase. Strains harboring fusions of the ech42 or nag1 5' upstream noncoding sequences with the A. niger goxA gene displayed a glucose oxidase activity pattern that was consistent under various conditions with expression of the native ech42 and nag1 genes, as assayed by Northern analysis. The expression product of goxA in the mutants was completely secreted into the medium, detectable on Western blots, and quantifiable by enzyme-linked immunosorbent assay. nag1 gene expression was triggered during growth on fungal (Botrytis cinerea) cell walls and on the chitin degradation product N-acetylglucosamine. N-Acetylglucosamine, di-N-acetylchitobiose, or tri-N-acetylchitotriose also induced nag1 gene expression when added to mycelia pregrown on different carbon sources. ech42 expression was also observed during growth on fungal cell walls but, in contrast, was not triggered by addition of chitooligomers to pregrown mycelia. Significant ech42 expression was observed after prolonged carbon starvation, independent of the use of glucose or glycerol as a carbon source, suggesting that relief of carbon catabolite repression was not involved in induction during starvation. In addition, ech42 gene transcription was triggered by physiological stress, such as low temperature, high osmotic pressure, or the addition of ethanol. Four copies of a putative stress response element (CCCCT) were found in the ech42 promoter.  (+info)

Strictures in Crohn's disease are characterised by an accumulation of mast cells colocalised with laminin but not with fibronectin or vitronectin. (2/420)

BACKGROUND/AIMS: Intestinal fibrosis and stricture formation is an unresolved problem in Crohn's disease. The aim of this study was to investigate whether mast cells accumulate in these tissues and whether their localisation is associated with extracellular matrix components. METHODS: Mast cells were visualised by immunohistochemical staining of the mast cell specific proteases chymase and tryptase. Their localisation in relation to extracellular matrix components was shown by immunohistochemical double labelling. RESULTS: In strictures in Crohn's disease, a striking accumulation of mast cells was seen particularly in the hypertrophied and fibrotic muscularis propria, with a mean (SEM) mast cell number of 81.3 (14.9) v 1.5 (0.9)/mm(2) in normal bowel (p<0.0005). All mast cells in the muscularis propria were colocalised with patches of laminin. In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. No colocalisation of mast cells was found with fibronectin or vitronectin. CONCLUSIONS: The large accumulation of mast cells in the muscle layer of strictured bowel suggests a functional role for these cells in the hypertrophic and fibrotic response of the smooth muscle cells. The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis.  (+info)

Hydrogen peroxide production from reactive liposomes encapsulating enzymes. (3/420)

Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated in combination with horseradish peroxidase (HRP) or lactoperoxidase (LPO). The generation of hydrogen peroxide from the liposomes in response to externally added D-glucose substrate was monitored using a Rank electrode system polarised to +650 mV, relative to a standard silver-silver chloride electrode. The effects of encapsulated enzyme concentration, enzyme combinations (GO+HRP, GO+LPO), substrate concentration, electron donor and temperature on the production of hydrogen peroxide have been investigated. The electrode signal (peroxide production) was found to increase linearly with GO incorporation, was reduced on addition of HRP and an electron donor (o-dianisidine) and showed a maximum at the lipid chain-melting temperature from the anionic liposomes containing no cholesterol. To aid interpretation of the results, the permeability of the non-reactive substrate (methyl glucoside) across the bilayer membranes was measured. It was found that the encapsulation of the enzymes effected the permeability coefficients of methyl glucoside, increasing them in the case of anionic liposomes and decreasing them in the case of cationic liposomes. These observations are discussed in terms of enzyme bilayer interactions.  (+info)

Immunotargeting of glucose oxidase: intracellular production of H(2)O(2) and endothelial oxidative stress. (4/420)

Extracellular and intracellular reactive oxygen species attack different targets and may, therefore, result in different forms of oxidative stress. To specifically study an oxidative stress induced by a regulated intracellular flux of a defined reactive oxygen species in endothelium, we used immunotargeting of the H(2)O(2)-generating enzyme glucose oxidase (GOX) conjugated with an antibody to platelet-endothelial cell adhesion molecule (PECAM)-1, an endothelial surface antigen. Anti-PECAM-(125)I-GOX conjugates specifically bind to both endothelial and PECAM-transfected cells. Approximately 70% of cell-bound anti-PECAM-(125)I-GOX was internalized. The cell-bound conjugate was enzymatically active and generated H(2)O(2) from glucose. Use of the fluorescent dye dihydrorhodamine 123 revealed that 70% of H(2)O(2) was generated intracellularly, whereas 30% of H(2)O(2) was detected in the cell medium. Catalase added to the cells eliminated H(2)O(2) in the medium but had little effect on the intracellular generation of H(2)O(2) by anti-PECAM-GOX. Both H(2)O(2) added exogenously to the cell medium (extracellular H(2)O(2)) and that generated by anti-PECAM-GOX caused oxidative stress manifested by time- and dose-dependent irreversible plasma membrane damage. Inactivation of cellular catalase by aminotriazole treatment augmented damage caused by either extracellular H(2)O(2) or anti-PECAM-GOX. Catalase added to the medium protected either normal or aminotriazole-treated cells against extracellular H(2)O(2), yet failed to protect cells against injury induced by anti-PECAM-GOX. Therefore, treatment of PECAM-positive cells with anti-PECAM-GOX leads to conjugate internalization, predominantly intracellular H(2)O(2) generation and intracellular oxidative stress. These results indicate that anti-PECAM-GOX 1) provides cell-specific intracellular delivery of an active enzyme and 2) causes intracellular oxidative stress in PECAM-positive cells.  (+info)

Expression of amylase and glucose oxidase in the hypopharyngeal gland with an age-dependent role change of the worker honeybee (Apis mellifera L.). (5/420)

Worker honeybees change their behaviour from the role of nurse to that of forager with age. We have isolated cDNA clones for two honeybee (Apis mellifera L.) genes, encoding alpha-amylase and glucose oxidase homologues, that are expressed in the hypopharyngeal gland of forager bees. The predicted amino acid sequence of the putative Apis amylase showed 60.5% identity with Drosophila melanogaster alpha-amylase, whereas that of Apis glucose oxidase showed 23.8% identity with Aspergillus niger glucose oxidase. To determine whether the isolated cDNAs actually encode these enzymes, we purified amylase and glucose oxidase from homogenized forager-bee hypopharyngeal glands. We sequenced the N-terminal regions of the purified enzymes and found that they matched the corresponding cDNAs. mRNAs for both enzymes were detected by Northern blotting in the hypopharyngeal gland of the forager bee but not in the nurse-bee gland. These results clearly indicate that expression of the genes for these carbohydrate-metabolizing enzymes, which are needed to process nectar into honey, in the hypopharyngeal gland is associated with the age-dependent role change of the worker.  (+info)

Second harmonic generation of glucose oxidase at the air/water interface. (6/420)

We present a study of the adsorption of the glucose oxidase enzyme (GOx) at the air/water interface, using the nonlinear optical technique of surface second harmonic generation (SSHG). Resonant SSHG experiments were achieved by probing the pi-pi* transition of the flavin adenine dinucleotide (FAD) chromophores embedded in the GOx protein. Because of the subsequent resonance enhancement of the signal, the second harmonic (SH) wave arising from the GOx entities adsorbed at the interface was detectable for protein bulk aqueous concentrations as low as 70 nM. The protein adsorption was followed, and, at high GOx coverage, a change in the orientation of the FAD chromophore was observed, indicating either a rearrangement or a reorientation of the protein at the interface. Inasmuch as GOx is negatively charged at the biological pH of 7, its interactions with charged surfactants were also investigated. As expected, spreading positively charged surfactants onto a partial protein monolayer was found to increase the GOx surface concentration, whereas in the case of negatively charged surfactants, the GOx surface concentration decreased until the SH signal went back to the pure buffer solution response level. With the increasing GOx surface concentration, the rearrangement or reorientation of the protein was also observed.  (+info)

Imaging of biological macromolecules on mica in humid air by scanning electrochemical microscopy. (7/420)

Imaging of DNA, keyhole limpet hemocyanin, mouse monoclonal IgG, and glucose oxidase on a mica substrate has been accomplished by scanning electrochemical microscopy with a tungsten tip. The technique requires the use of a high relative humidity to form a thin film of water on the mica surface that allows electrochemical reactions to take place at the tip and produce a faradaic current (approximately 1 pA) that can be used to control tip position. The effect of relative humidity and surface pretreatment with buffer solutions on the ionic conductivity of a mica surface was investigated to find appropriate conditions for imaging. Resolution of the order of 1 nm was obtained.  (+info)

Conserved arginine-516 of Penicillium amagasakiense glucose oxidase is essential for the efficient binding of beta-D-glucose. (8/420)

The effects of mutation of key conserved active-site residues (Tyr-73, Phe-418, Trp-430, Arg-516, Asn-518, His-520 and His-563) of glucose oxidase from Penicillium amagasakiense on substrate binding were investigated. Kinetic studies on the oxidation of beta-D-glucose combined with molecular modelling showed the side chain of Arg-516, which forms two hydrogen bonds with the 3-OH group of beta-D-glucose, to be absolutely essential for the efficient binding of beta-D-glucose. The R516K variant, whose side chain forms only one hydrogen bond with the 3-OH group of beta-D-glucose, exhibits an 80-fold higher apparent K(m) (513 mM) but a V(max) only 70% lower (280 units/mg) than the wild type. The complete elimination of a hydrogen-bond interaction between residue 516 and the 3-OH group of beta-D-glucose through the substitution R516Q effected a 120-fold increase in the apparent K(m) for glucose (to 733 mM) and a decrease in the V(max) to 1/30 (33 units/mg). None of the other substitutions, with the exception of variant F418A, affected the apparent K(m) more than 6-fold. In contrast, the removal of aromatic or bulky residues at positions 73, 418 or 430 resulted in decreases in the maximum rates of glucose oxidation to less than 1/90. Variants of the potentially catalytically active His-520 and His-563 were completely, or almost completely, inactive. Thus, of the residues forming the active site of glucose oxidase, Arg-516 is the most critical amino acid for the efficient binding of beta-D-glucose by the enzyme, whereas aromatic residues at positions 73, 418 and 430 are important for the correct orientation and maximal velocity of glucose oxidation.  (+info)

Enzyme-based sensors have emerged as important analytical tools with application in diverse fields, and biosensors for the detection of glucose using the enzyme glucose oxidase have been widely investigated. In this work, the preparation of biosensors by electrochemical polymerization of (poly)thiophenes, namely 2,2′-bithiophene (2,2′-BT) and 4,4′-bis(2-methyl-3-butyn-2-ol)-2,2′-bithiophene (4,4′-bBT), followed by immobilization of glucose oxidase on the films, is described. N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide metho-|i|p|/i|-toluenesulfonate (CMC) was used as a condensing agent, and |i|p|/i|-benzoquinone (BQ) was used as a redox mediator in solution. The glucose oxidase electrodes with films of 2,2′-BT and 4,4′-bBT were then tested for their ability in detecting glucose from synthetic and real samples (pear, apricot, and peach fruit juices).
Research on lab-on-a-chip devices fabricated by traditional microfabrication techniques are gaining importance in the field of analytical chemistry. In particular there is an increasing demand for miniaturized biosensors based on enzymes owing to their applications in clinical analysis. With respect to Capillary Electrophoresis (CE) microchips, enzymes can impart a greater degree of selectivity. The technique of electrochemical polymerization was used to immobilize glucose oxidase on the CE microchip. Polypyrrole was used an immobilization matrix. The second chapter describes the experimental procedure for the immobilization of glucose oxidase on the CE microchip device. A two-step procedure was employed, which involved a thin layer of polypyrrole followed by a thick layer of polypyrrole/glucose oxidase film. The third chapter deals with the results obtained using a CE microchip ...
This study aimed to verify the performance of xylanase and its interaction with oxidants agents (glucose oxidase and ascorbic acid) on the quality of whole wheat bread. The experiment was based on a central composite rotational design and the Response Surface Methodology was used to analyze the results. None of the xylanase, glucose oxidase or ascorbic acid concentrations within the studied range led to a significant difference in the specific volume. The highest moisture content and the lowest firmness values were reported in the bread with lower and intermediate levels of xylanase and larger amounts of glucose oxidase and ascorbic acid. This effect was observed mainly at the end of the storage period. A minimum amount of xylanase (from 33 to 63 EDX kg-1 flour) showed to be essential for obtaining best results. Levels of ascorbic acid above 63mg kg-1 and glucose oxidase above 91 SRU kg-1 proved to be necessary to offer the beneficial effect of xylanase. © 2016, Universidade Federal de Santa ...
Find right answers right now! What are the advantages and disadvantages of the glucose oxidase method used for the measurement of glucose concentration in human samples? More questions about Science & Mathematics, what
Global Glucose Oxidase Market is estimated to be valued US$ XX.X million in 2019. The report on Glucose Oxidase Market provides qualitative as well as quantitative analysis in terms of market dynamics, competition scenarios, opportunity analysis, market growth, etc. for the forecast year up to 2029. The global glucose oxidase market is segmented on the basis of Type, Application, and geography ...
Microporous glass ceramic with skeleton of CaTi4(PO¬4)6 with average pore size of 12.7 nm has been synthesized and used as a carrier of glucose oxidase. The glass ceramic was prepared by controlled heat treatment of glass samples, which causes the phase separation in their structure and creates CaTi4(PO¬4)6 and β-Ca3(PO4)2 phases. The β-Ca3(PO4)2 phase was dissolved by soaking the glass ceramics in HCl and CaTi4(PO¬4)6 built the skeleton of microporous glass ceramic. Analysis of the ability of the carrier for immobilization of glucose oxidase (GOx) was undertaken. Average amount of immobilized enzyme and percentage of enzyme activity on the carrier were 27 gr GOx/gr carrier and 60.15%, respectively. Effect of pH and temperature variations on the enzyme activity has been studied and results demonstrated that maximum activity for both free and immobilized enzyme was at T=40˚C and pH=7.0. Due to the same value of maximum activity, no serious conformational change of enzyme had taken place through
Effects of Wheat Flour Dough’s Viscoelastic Level by Adding Glucose Oxidase on its Dynamic Shear Properties whatever the Strain Modes
Abstract: Nano-carbon aerogels (CA) were prepared by the reaction between resorcinol and formaldehyde in alkaline conditions. The morphology and microstructure of the CA was investigated by scanning electron microscopy and transmission electron microscopy. The specific area and the pore size distribution were calculated from the N2 adsorption-desorption isotherm. Glucose oxidase (GOD)/CA/ glassy carbon (GC) electrodes were prepared by immobilizing GOD on the surface of nano-carbon aerogels. The electrocatalytic properties of GOD/CA/GC electrodes were characterized by cyclic voltammetry in phosphate buffer medium (0.1 mol·L-1). The results showed that GOD can be immobilized on the nano-carbon aerogels, and that the biological activation of GOD was retained. The GOD/CA/GC electrode exhibits good direct electrochemical and electro-catalytic performance without electron mediators.. Key words: Carbon aerogel, Glucose oxidase, Direct electrochemistry ...
TY - JOUR. T1 - Kinetic limitations of a bioelectrochemical electrode using carbon nanotube-attached glucose oxidase for biofuel cells. AU - Zhao, Xueyan. AU - Jia, Hongfei. AU - Kim, Jungbae. AU - Wang, Ping. PY - 2009/12/15. Y1 - 2009/12/15. N2 - Carbon nanotubes (CNTs) have been used for various bioelectrochemical applications, presumably for substantial improvement in performance. However, often only moderate results observed, with many governing factors have been considered and suggested yet without much systematic evaluation and verification. In this study, CNT-supported glucose oxidase (CNT-GOx) was examined in the presence of 1,4-benzoquinone (BQ). The intrinsic Michaelis parameters of the reaction catalyzed by CNT-GOx were found very close to those of native GOx. However, the Nafion entrapment of CNT-GOx for an electrode resulted in a much lower activity due to the limited availability of the embedded enzyme. Interestingly, kinetic studies revealed that the biofuel cell employing such ...
This article presents a study of glucose oxidase (GOx) immobilization by employing tannic acid (TA) modified-CoFe2O4 (CFO) magnetic nanoparticles which demonstrates novel aspect for enzyme immobilization. By using the strong protein and tannic acid binding, GOx immobilization was carried out via physical adsorption in a simpler way compared with the other immobilization methods which require various chemicals and complicated procedures which is difficult, expensive, time-consuming, and destructive to the enzyme structure. CFO was synthesized by hydrothermal synthesis and modified with TA to immobilize GOx. The immobilized GOx demonstrated maximum catalytic activity at pH 6.5 and 45°C. The samples were characterized by vibrating sample magnetometer (VSM), thermogravimetric analysis (TGA), zeta potential, and fourier transform infrared spectroscopy (FTIR), all of which confirm the surface modification of CFO and GOx immobilization. Also, field emission scanning electron microscope (FESEM) and ...
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It is known that regulation of plant tolerance to adverse environmental factors is connected with short term increase of the concentration of endogenous reactive oxygen species (ROS), which are signalling molecules for the induction of protective mechanisms. Introduction and expression of heterologous gox gene, which encodes glucose oxidase enzyme in plant genome, induce constantly higher content of hydrogen peroxide in plant tissues. It is not known how the introduction of native or modified gox gene affects the plant resistance to high-temperature stress, one of the most commonly used model for the study of stress response and thermal tolerance. In this study, we investigated biological effects of transformation and evaluated the resistance to temperature stress of potato plants with... altered levels of glucose oxidase expression. Transformation of potato plants by gox gene led to the more early coming out from tuber dormancy of transformed plants and slower growth rate. Transformants ...
Based on electric conductivity and wide potential window of ionic liquid (IL) and electric property of single-wall car- bon nanotubes (SWCNTs), composite material of IL-SWCNTs was prepared, glucose sensor was built with this mate-rial for immobilizing glucose oxidase (GOx). It showed good response, sensitivity and stability for long time for glu-cose detection. Linear range for the detection of glucose was from 0.5 × 10-6 M to 12 × 10-6 M while detection limit was 6.26 × 10-8 M (S/N = 3).
Title:Analyzing the Amperometric Response of a Glucose Oxidase Sensor Applying Mathematical Models. VOLUME: 12 ISSUE: 6. Author(s):Felix F. González Navarro, Margarita Stoytcheva, Lluís A. Belanche Munoz, Brenda L. Flores Rios, Jorge E. Ibarra Esquer, Livier Rentería Gutiérrez and Gabriel A. López Morteo. Affiliation:Engineering Institute, Autonomous University of Baja California; Mexicali, Mexico.. Keywords:Glucose oxidase amperometric sensor, machine learning, regression models, biosensor modeling.. Abstract:Background: The biosensors are analytical devices combining a bioreceptor and a physicochemical transducer to translate the signal resulting from the interaction of the analyte with the biological element into an electrical reading. The technological attractiveness of the biosensors resides in their high sensitivity, continuous measuring capabilities, low cost, and precision. An important and promising area of biosensors application is the associated with blood glucose levels ...
A thin-walled graphitic nanocages material with well-developed graphitic structure, large specific surface area and pronounced mesoporosity was synthesized and used to construct a sensing interface for an amperometric glucose biosensor, showing a high and reproducible sensitivity of 13.3 μA mM−1 cm−2, linear dynamic range of 0.02−6.2 mM, and fast response time of 5 s. It was successfully used to accurately detect glucose in human serum with effective discrimination to common interference species such as dopamine, ascorbic acid, acetaminophen, and uric acid ...
Antibacterial ? How long ? and what if diluted ?. Honey is claimed to be anti microbial by osmosis (high concentration on particles such as glucose and saccharose) and by producing Hydrogen peroxide (H2O2) when in contact with water. The latter is because some honeys do contain the enzyme glucose oxidase that converts glucose in glucon acid and H2O2. Hydrogen peroxide is a potent anti microbial agent that is effective in small quantities against most microorganisms. If honey is heated or irradiated the glucose oxidase will be destroyed.. To check if the glucose oxidase is active enough even when the Melladerm® Plus is diluted heavily we ordered a Zone of inhibition test in a certified independent laboratory.. Zone of Inhibition test. A zone of inhibition test was performed showing that Melladerm® Plus could be diluted 30 times before losing its antibacterial activity against Staphylococcus aureus. To execute the actual assay, we choose a LMG-reference culture, i.e. Staphylococcus aureus subsp. ...
A wearable-type glucose sensor for continuously or intermittently determining the glucose content comprises a short hollow fiber (2) to be positioned in the subcutaneous tissue. This hollow fiber is connected via tubes (3,4) with component parts (5 . . . 12) located outside the body, such as a measuring unit (11). When a perfusion fluid containing the enzyme glucose oxidase is passed through the hollow fiber, a subcutaneous dialysis will take place in which some glucose dissolves in the perfusion fluid through the wall of the hollow fiber. This glucose is completely oxidized by the oxygen dissolved in the perfusion fluid in the presence of the glucose oxidase. By means of the measuring unit the resultant amount of H2 O2 or, preferably, the employed amount of O2 is determined, both of which are a measure of the subcutaneous glucose concentration.
An interesting observation of this study is that insulin-induced Rac activation and actin remodeling appear to be more sensitive to disruption by glucose oxidase than is Akt phosphorylation: Rac activation was reduced by ,60% at 12.5 mU/ml glucose oxidase (Fig. 4), whereas Akt phosphorylation at either Thr308 or Ser 473 had only begun to be affected by twice those concentrations (Fig. 6). A slight difference was also observed with C2-ceramide, which at 12.5 μmol/l effectively reduced Rac activation, yet Akt Ser473 phosphorylation remained intact. However, Thr308 phosphorylation was as sensitive to C2-ceramide as was Rac activation.. Insulin-induced GLUT4 translocation was also somewhat more susceptible to inhibition by glucose oxidase and C2-ceramide than was Akt phosphorylation. Defects in GLUT4 translocation were apparent at 12.5 mU/ml glucose oxidase or 12.5 μmol/l C2-ceramide, and complete inhibition was achieved with 50 mU/ml glucose oxidase or 50 μmol/l C2-ceramide, concentrations that ...
An interesting observation of this study is that insulin-induced Rac activation and actin remodeling appear to be more sensitive to disruption by glucose oxidase than is Akt phosphorylation: Rac activation was reduced by ,60% at 12.5 mU/ml glucose oxidase (Fig. 4), whereas Akt phosphorylation at either Thr308 or Ser 473 had only begun to be affected by twice those concentrations (Fig. 6). A slight difference was also observed with C2-ceramide, which at 12.5 μmol/l effectively reduced Rac activation, yet Akt Ser473 phosphorylation remained intact. However, Thr308 phosphorylation was as sensitive to C2-ceramide as was Rac activation.. Insulin-induced GLUT4 translocation was also somewhat more susceptible to inhibition by glucose oxidase and C2-ceramide than was Akt phosphorylation. Defects in GLUT4 translocation were apparent at 12.5 mU/ml glucose oxidase or 12.5 μmol/l C2-ceramide, and complete inhibition was achieved with 50 mU/ml glucose oxidase or 50 μmol/l C2-ceramide, concentrations that ...
In this paper we show that hyperbranched polymers can be used as a host matrix for electrostatic entrapment of enzymes. Specifically, amine-functionalized glucose oxidase (GOx+) and horseradish peroxidase, as well as poly(amidoamine) dendrimer-modified horseradish peroxidase, reversibly sorb into polyanionic, hyperbranched poly(sodium acrylate) (PAA-) films that are on the order of a few hundred angstroms thick. The quantity of GOx+ entrapped within the PAA- films depends on the nature of film preparation but is typically on the order of 0.06 unit/cm2. The extent to which entrapped GOx+ retains its activity depends on the film history, but for PAA-/GOx+ composites not exposed to glucose and stored at 4 degrees C, the original activity is retained for up to 68 days and perhaps longer.
Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
The sugar oxidising enzymes glucose oxidase, glucose dehydrogenases (GDH) and cellobiose dehydrogenases (CDH) were co-immobilised, in the presence of multiwalled carbon nanotubes, with osmium redox polymers. Under pseudo-physiological conditions of 5 mM glucose, 150 mM NaCl, 37?degrees C, glucose oxidation current densities above 800 mu A?cm-2 are obtained from films containing an [Os(4,4xxx-dimethyl-2,2xxx-bipyridine)2(poly-vinylimidazole)10Cl]+ redox polymer, redox potential 0.1 V vs. Ag/AgCl, and either glucose oxidase or FAD-dependant GDH. Current produced by, and stability of, glucose-oxidising half-cells is compared in 100 mM glucose, with films containing CDHs proving most stable. Such results show promise for development of glucose-oxidising enzymatic fuel cells ...
In this work, a novel chemiluminescence (CL) flow biosensor for glucose was proposed. Glucose oxidase (GOD), horseradish peroxidase (HRP) and gold nanoparticles were immobilized with sol-gel method on the inside surface of the CL flow cell. The CL de
A gene of glucose oxidase (GOD) from Aspergillus niger Z-25 was cloned and sequenced. The entire open reading frame (ORF) consisted of 1,818 bp and encoded a putative peptide of 605 amino acids. The gene was fused to the pPICZαA plasmid and overexpressed in Pichia pastoris SMD1168. The recombinant GOD (rGOD) was secreted into the culture using MF-α factor signal peptide under the control of the AOX1 promoter. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that rGOD exhibited a single band at around 94 kDa. The maximal GOD activity of approximately 40 U/mL was achieved in shake flask by induction under optimal conditions after 7 days. rGOD was purified by ammonium sulfate precipitate leading to a final specific activity of 153.46 U/mg. The optimum temperature and pH of the purified enzyme were 40 °C and 6.0, respectively. Over 88% of maximum activity was maintained below 40 °C. And the recombinant enzyme displayed a favorable stability in the pH range from 4.0 to 8.0. The ...
glucose oxidase from aspergillus niger/ACM9001370 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
4665-7604 recognises glucose oxidase from Aspergillus niger.This antibody has not been cross-adsorbed and may react with homologous proteins
A glucose oxidase (GOd) bioelectrode exhibiting high performance, direct electron transfer (DET) has been prepared. Unprecedented redox peak current densities of 1 mA cm(-2) were observed alongside a clear electrochemical response to glucose. This system shows potential as a low cost, high performance enzymatic bioelectrode.. ...
Page contains details about three-dimensional nitrogen-doped graphene/chitosan/glucose oxidase . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
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View Notes - 0825 from BME 511 at USC. Desired blood glucose level Insulin Insulin-release Glucose Glucose sensor Measured blood glucose Questions to be answered : 1. Dynamics of the system -
1CF3: 1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes.
In vivo brain glucose measurements: differential normal pulse voltammetry with enzyme-modified carbon fiber microelectrodes.: The enzyme glucose oxidase was imm
Adiy bin Hatim berkata: Ketika turun ayat kelenjar ini adalah menghasilkan hormon insulin. Diabetic Aikido Acidosis List Grocery Store but our liary can be accessed from certain countries only. This classic Jamaican dish is low in fat yet high in fiber protein and calcium. Home , Managing Your Diabetes , Kitbags and carry cases. No More Diabetes Testing Using Blood? The diabetes test strips used in currently available glucose monitoring systems contain the enzyme glucose oxidase. Diabetes Research Projects Article Its a safe forum where you can create or participate in support groups and discussions about health topics that interest you.. Young-onset type 2 diabetes mellitus (T2DM) is associated with greater mortality increased complications and unfavorable cardiovascular risk versus type 1 diabetes The ideal for your sugar before you eat in the morning is Dr. Diabetes Symptoms Tingling Feet It seems keeping you sick and marginally pain-free is all any ones really to do for you doing so means ...
i had such an overwhelming response asking for this reference im just going to post it here for all. Andrew SM, Jasani B (1987) Histochem J 19:426-30 best of luck to all who try it....i think its a great protocol for use on frozens. it does take a bit of time....you incubate for 1hr. @ 37C. carrie kyle-byrne [email protected] ...
Suggested Use: After cleansing and toning your face in the evening, apply a thin layer of Hyaluronic Acid PM Creme to face and neck. Use daily for best results. Ingredients: De-ionized Water, Stearic Acid (vegetable source), Avocado Oil, Glyceryl Stearate, Cetyl Alcohol, Vegetable Glycerin, Cetearyl Alcohol/Cetearyl Glucoside, Palmitic Acid Ester, Vegetable Squalane, Soy Lecithin, Natural Aroma, Glucose/Glucose Oxidase/Lactoperoxidase (natural preservative), Cetyl Palmitate, Shea Butter (tree nut), Manago Butter, Jojoba Oil, Sodium Hyaluronate (Hyaluronic Acid), Potassium Sorbate, MSM, L-Carnosine, Xanthan Gum, Tetrahexyldecyl Ascorbate (Vitamin C), d-alpha Tocopherol (Vitamin E), Soluble Collagen, Grapefruit Seed Extract and Green Tea Extract. NOW does not test on animals. Please Recycle ...
An immobilized enzyme electrode effective in measurement of the substrate concentration of the enzyme and in conversion from enzyme reaction energies into electric energies. The immobilized enzyme, of an oxidase system, such as glucose oxidase, amino acid oxidase, xanthine oxidase or the like and a metal oxide capable of constituting a redox system which is reduced through coupling with these enzyme reactions and is electrochemically oxidized (anodic oxidation) are combined with each other. The use of the enzyme electrode allows the determination quantity of the enzyme substrate as extremely low as approximately 10-5 to 10-6 mole/l in concentration.
OBJECTIVE: To determine the day-to-day intraindividual variability of fasting plasma glucose (FPG) in newly diagnosed Caucasian type 2 diabetic subjects. RESEARCH DESIGN AND METHODS: A total of 193 newly diagnosed, previously untreated, Caucasian type 2 diabetic subjects (135 men, 58 women) had FPG measured on two consecutive days (FPG1, FPG2). Ethical approval and subjects full informed consent were obtained. Subjects fasted for 12 h before each study day and rested for at least 30 min before blood was taken. Plasma glucose was analyzed by a glucose oxidase method with intra- and interassay coefficients of variation (CVs) , 2%. Variability of FPG was assessed by comparison of percentage differences (PDs): PD = 100 (FPG2 - FPG1)/FPG1, with averaged FPG (FPGaver = [FPG1 + FPG2]/2). Biological and analytical variability were determined by use of SD2total = SD2biological + SD2analytical, where SD2analytical approximately equal to 2 x (CVglucose measurement)2. Given normally distributed data with ...
There is a peroxidase quench that will block ALL forms of peroxidase, totally. This is the glucose oxidase method. Hsu H-M, et al Am J Pathology 188:209-217,1984 Andrew SM and Jasani G Histchem J 19:426-430, 1987 Vector provided me with the exact protocol ,Date: Mon, 10 Jul 2000 14:43:03 -0400 ,From: Jeff Crews ,[email protected], ,Subject: Re: IH on Liver Tissue ,To: [email protected], [email protected] , , With extremely bloody tissue like spleen or liver, it is sometimes , impossible to quench all of the peroxidase present in the blood. Try , an alkaline-phoshatase detection system. , , Jeffrey Crews, HTL (ASCP) , Organogenesis, Inc. , , ,______________________________ Reply Separator _________________________________ ,Subject: IH on Liver Tissue ,Author: ,[email protected], at internet ,Date: 07/10/2000 11:03 AM , , ,How can you successfully perform immunohistochemistry on slides of liver ,tissue using horse-radish peroxidase and DAB? How do you quench the ,endogenous peroxidase ...
ice for 5 min to avoid coagulation and then centrifuged at 12 0006 g for 2 min at 4uC to pellet the hemocytes. The supernatant was then collected. Glucose concentration (mg dL21) in the hemolymph was assessed using the glucose oxidase method of a commercial kit (Hospitex Diagnostics).Criteria for Choosing Experimental CrayfishOnly hard-shelled, intact, and sexually mature males were used for the experiment. A total of 80 individuals (cephalothorax length: 47.560.6 mm) were thus selected: 20 for the extraction of cHH and 60 for behavioural observations. Since purchase Salmon calcitonin dominance increases with body size in crayfish [3], the experimental pairs of fighting males were size matched (maximum difference in cephalothorax length: 62 mm) to eliminate any factor that could induce an obvious bias to our experiments. Before the beginning of the experiment, crayfish were kept in isolation in opaque plastic aquaria (25615625 cm) for at least two weeks, which is a sufficient time to reset any ...
Plasma-glucose levels have been determined by a glucose oxidase method in newborn infants of normal mothers (INM) and of diabetic mothers (IDM).
Sensitive Enzymatic Assay for Glucose Determination in Natural Waters 1: A new enzymatic method for glucose determination is described. It allows measurement of
A non-mediated enzyme electrode comprises a base substrate (2) on which is provided an electrically conductive base layer (8) comprising finely divided platinum group metal or oxide bonded together by a resin; a top layer on the base layer (8), the top layer comprising a buffer. A catalytically active quantity of an oxidoreductase enzyme is provided in at least one of the base layer and the top layer. The invention also provides a biosensor (20) which includes an enzyme electrode, and methods of manufacturing the enzyme electrode and biosensor.
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Glucose Oxidase (GO) enzyme (~200-300 u/mg) Pure Protein GO11-N-250 Glucose Oxidase (GO) enzyme (~200-300 u/mg) Pure Protein GO11-N-250
A team led by Tom Soh, a Project Leader for The Institute for Collaborative Biotechnologies has fashioned a small electronic device that can continuously track the level of medicines in an animals bloodstream. If it works in people, the device could revolutionize how medicines such as anticancer compounds and antibiotics are monitored and administered for life-threatening conditions.. Doctors always aim to give their patients the right dose of medications. But thats often not easy. Drug levels typically spike above the desired level after a medicine is administered and then fall below it as time goes on. Its been even harder to track drugs with a continuous electronic readout. A device does exist for measuring glucose in the bloodstream of diabetics. It works by tethering an enzyme called glucose oxidase next to an electrode. When a drop of blood is added to the device, the glucose oxidase splits glucose molecules, swiping electrons in the process. Those electrons then hop to the electrode, ...
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2014-12-4 · Historical assay formats for point-of-care testing are dipsticks and lateral flow devices. In the 1950s, the earliest paper-based strip test emerged for the quantification of the concentration of glucose in urine, and this product was marketed in the 1960s [].The principle of operation of the assay was based on glucose oxidase, and developed colours were compared to a reference chart …. Get Price ...
In the glucose oxidase assay, the glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen ... The glucose oxidase binds specifically to β-D-glucopyranose and does not act on α-D-glucose. It oxidises all of the glucose in ... "Glucose Oxidase: A much used and much loved enzyme in biosensors" at University of Paisley Glucose+Oxidase at the US National ... Glucose oxidase catalyzes the oxidation of β-D-glucose into D-glucono-1,5-lactone, which then hydrolyzes into gluconic acid. In ...
The factor was glucose oxidase. In the presence of glucose, the glucose oxidase produced hydrogen peroxide, which killed off ... "Detlev Müller discovers glucose oxidase". Tacomed.com. Archived from the original on 18 April 2018. Retrieved 13 June 2017. ... He is best known for discovering the enzyme glucose oxidase. In 1928, he was experimenting with the common fungus, Aspergillus ... He eventually found that these bacteria could only thrive adjacent to Aspergillus niger if glucose was not present. He ...
... trapping the enzyme glucose oxidase. The surrounding solution would end up containing the enzyme lipase B with the glucose ... The resulting glucose would cross the membrane, become oxidized, and the horseradish peroxidase would convert the sample ...
In a study by Oxenbøll et al., C. oxysporum catalyzed glucose oxidation by producing glucose oxidase, which it is mentioned in ... that the glucose oxidase helped protect against bacterial infection on the surface of fungi. The organism is also proven to be ... Oxenbøll, K; Si, J. Q; Aagaard, J (3 May 1995). "Alkaline glucose oxidase obtained from Cladosporium oxysporum". US Grant. ... It breaks down cellulose from dead herbaceous or plants, which is then further turned into glucose to Be used by the fungi ...
"Thermal stability of Penicillium adametzii glucose oxidase". Prikladnaia biokhimiia i mikrobiologiia. 37 (6): 678-86. PMID ...
Many glucose meters employ the oxidation of glucose to gluconolactone catalyzed by glucose oxidase (sometimes known as GOx). ... Glucose vs. plasma glucose: Glucose levels in plasma (one of the components of blood) are higher than glucose measurements in ... The glucose in the blood reacts with an enzyme electrode containing glucose oxidase (or dehydrogenase). The enzyme is ... This is important because home blood glucose meters measure the glucose in whole blood while most lab tests measure the glucose ...
Other work concerned enzymes such as "diaphorase", glucose oxidase, cytochrome oxidase and peroxidase. Much of Gibson's work ... Gibson, Q H; Massey, V; Swoboda, B E P J (1964). "Kinetics and mechanism of action of glucose oxidase". J. Biol. Chem. 239 (11 ... Gibson, Q H; Greenwood, C; Wharton, D C; Palmer, G (1965). "Reaction of cytochrome oxidase with cytochrome C". J. Biol. Chem. ... Brunori, M.; Gibson, Q.H. (1983). "Oxygen activation by cytochrome oxidase: A new spectral intermediate observed by flow-flash ...
"Glucose oxidase-magnetite nanoparticle bioconjugate for glucose sensing". Analytical and Bioanalytical Chemistry. 380 (4): 606- ...
... glucose oxidase (P13006), used in the design of glucose biosensors, due to its high affinity for β-D-glucose. In the food ... "Nano-Encapsulation of Glucose Oxidase Dimer by Graphene". RSC Advances. 5 (18): 13570-78. doi:10.1039/C4RA16852F. S2CID ... Staiano M, Bazzicalupo P, Rossi M, D'Auria S (December 2005). "Glucose biosensors as models for the development of advanced ...
Commercially available glucose monitors rely on amperometric sensing of glucose by means of glucose oxidase, which oxidises ... which uses the enzyme glucose oxidase to break blood glucose down. In doing so it first oxidizes glucose and uses two electrons ... "Nano-Encapsulation of Glucose Oxidase Dimer by Graphene". RSC Advances. 5 (18): 13570-78. doi:10.1039/C4RA16852F. Daaboul, G.G ... Measured glucose data will be transmitted wirelessly out of the body within the MICS 402-405 MHz band as approved for medical ...
Recently, pyranose oxidase has been gaining on popularity within biosensors. Unlike glucose oxidase, it can produce higher ... Other names in common use include glucose 2-oxidase, and pyranose-2-oxidase. This enzyme participates in pentose phosphate ... In enzymology, a pyranose oxidase (EC 1.1.3.10) is an enzyme that catalyzes the chemical reaction D-glucose + O2 ⇌ {\ ... whereas its two products are 2-dehydro-D-glucose and H2O2. Pyranose oxidase is able to oxidize D-xylose, L-sorbose, D-galactose ...
CHOD = cholesterol oxidase GOD = glucose oxidase GPO = glycerol-3-phosphate oxidase PAP = phenol + aminophenazone (in some ... water Glucose + oxygen + water --(enzyme glucose oxidase)--> gluconate + hydrogen peroxide Hydrogen peroxide + ABTS --(enzyme ... Cholesterol + oxygen --(enzyme cholesterol oxidase)--> cholestenone + hydrogen peroxide Hydrogen peroxide + 4-aminophenazone + ... enzyme glycerol-3-phosphate oxidase) --> dihydroxyacetone phosphate + hydrogen peroxide Hydrogen peroxide + 4-aminophenazone + ...
Glucose oxidase-like gold nanoparticles combined with cyclodextran were used for chiral catalysis. An artificial binuclear ... Glucose oxidase and iron oxide nanozymes were encapsulated within multi-compartmental hydrogels for incompatible tandem ... A Pd12 coordination cage as a photoregulated oxidase-like nanozyme was developed. An NADPH oxidase-like nanozyme was developed ... with glucose oxidase (GOx) in sub-micron proximity, providing a fast and efficient enzyme cascade reported as monitoring ...
"Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics". Biosens. Bioelectron. 50: 84-90. ... glucose oxidase for analytical application, phytase with increased thermostability and monooxygenase with improved catalytic ...
For example , the immobilization of glucose oxidase on calcium alginate gel beads can be used in a bioreactor. The resulting ... Examples include entrapment of enzymes such as glucose oxidase in gel column for use as a bioreactor. Important characteristic ... "Production of gluconic acid with immobilized glucose oxidase in airlift reactors". Chemical Engineering Science. 52 (21-22): ... Kinetic studies of Immobilized glucose oxidase". Biotechnology and Bioengineering. 16 (2): 159-168. doi:10.1002/bit.260160202. ...
... bees use the enzyme glucose oxidase, stored in their hypopharyngeal glands. Glucose oxidase works to ... Burgett, D. Michael (January 9, 1974). "Glucose Oxidase: A Food Protective Mechanism in Social Hymenoptera" (PDF). Annals of ...
Biosensors/diagnostics, e.g. glucose oxidase and cholesterol oxidase biosensors. Delivery of proteins as therapeutic agents or ...
The three primary enzymes in Biotène are[were? no longer, per the above] Glucose Oxidase, Lactoperoxidase, and Lysozyme. ... and glucose oxidase. The PBF products have been discontinued. People with xerostomia (dry mouth) may use Biotène to reduce the ... and prior to the GlaxoSmithKline acquisition it also contained enzymes including glucose oxidase, lactoferrin, lactoperoxidase ...
Now, however, the membrane is impregnated with immobilized glucose oxidase (GOx). The GOx will consume some of the oxygen as it ... leaving as the only variable the oxygen and glucose concentrations on the analyte-side of the glucose membrane, which is the ... The Clark oxygen electrode laid the basis for the first glucose biosensor (in fact the first biosensor of any type), invented ... The rate of reaction current is limited by the diffusion of both glucose and oxygen. This diffusion can be well characterized ...
GNP-glucose oxidase monolayer electrode was constructed use these two methods. The Au NP allowed more freedom in the enzyme's ... These ribbons are very flexible and can bend with angles larger than 90°. When glucose is replaced by cyclodextrin (a glucose ... In another method using ultrasound, the reaction of an aqueous solution of HAuCl4 with glucose, the reducing agents are ... Large AuNPs conjugated with biotin, cysteine, citrate, and glucose were not toxic in human leukemia cells (K562) for ...
Petruccioli, M.; Federici, F. (1993). "Glucose oxidase production by Penicillium variabile P16: Effect of medium composition". ... "Expression of Penicillium variabile P16 glucose oxidase gene in Pichia pastoris and characterization of the recombinant enzyme ...
These glucose sensitive hydrogels are modified with the enzyme glucose oxidase. In the presence of glucose, the glucose oxidase ... An example of this would be hydrogels that release insulin in the presence of high glucose levels in the bloodstream. ...
"Glucose-sensitive membranes containing glucose oxidase: Activity, swelling, and permeability studies". Journal of Biomedical ... The major focus has been the development of a non-invasive continuous detection of glucose. The first clinical study on ... Since, Kost also proposed a novel approach for a glucose flux continuous biosensor and noninvasive detection of amniotic fluid ... In addition to his studies on responsive controlled release systems: pH sensitive, glucose sensitive and calcium sensitive drug ...
Studies of Enzyme-Mediator Kinetics for Membrane and Surface Immobilized Glucose Oxidase". Anal. Chem. 64 (17): 1795-1804. doi: ...
... and urea-induced unfolding of the dimeric enzyme glucose oxidase". Biochemistry. 41 (11): 3819-27. doi:10.1021/bi0116700. PMID ...
The extract has also shown significant inhibition against glucose oxidase-mediated inflammation. The observations have ...
Calibration of glucose oxidase-based test strips for capillary blood measurement with oxygen saturated venous blood samples. ... Oxidase-positive bacteria possess cytochrome oxidase or indophenol oxidase (an iron-containing hemoprotein). These both ... The oxidase test is used to determine if an organism possesses the cytochrome c oxidase enzyme. The test is used as an aid for ... Oxidase Test Protocol - Library. American Society for Microbiology, ASM MicrobeLibrary, 1-5. Steel K J. 1961. The Oxidase ...
2. Methods for bonding electron-transfer relays to glucose oxidase and D-amino-acid oxidase. J. Am. Chem. Soc. Vol. 110. pp. ... Using electrically wired glucose oxidase he and his team engineered prototypes of subcutaneously implanted continuously glucose ... "Design and Optimization of a Selective Subcutaneously Implantable Glucose Electrode Based on "Wired" Glucose Oxidase". ... Amperometric glucose microelectrodes prepared through immobilization of glucose oxidase in redox hydrogels. Anal. Chem. Vol. 63 ...
Roth JP, Klinman JP (January 2003). "Catalysis of electron transfer during activation of O2 by the flavoprotein glucose oxidase ... Leys D, Basran J, Scrutton NS (August 2003). "Channelling and formation of 'active' formaldehyde in dimethylglycine oxidase". ...
The Glucose oxidase of Penicillium amagasakiense has been studied in detail because of the use of Glucose oxidase in biosensors ... Zoldak, G.; Zubrik, A.; Musatov, A.; Stupak, M.; Sedlak, E. (2004). "Irreversible Thermal Denaturation of Glucose Oxidase from ... MycoBank ATCC UniProt Conserved arginine-516 of Penicillium amagasakiense glucose oxidase isessential for the efficient binding ... Courjean, O.; Mano, N. (2011). "Recombinant glucose oxidase from Penicillium amagasakiense for efficient bioelectrochemical ...
... acidity regulator Glucose oxidase - antioxidant Glucose syrup - sweetener Glutamate - Glutamic acid - flavor enhancer Gluten - ...
Legan M (August 2010). "Cyclooxygenase-2, p53 and glucose transporter-1 as predictors of malignancy in the development of ... PTGS (COX, which can be confused with "cytochrome oxidase") enzymes are monotopic membrane proteins; the membrane-binding ...
... where it can be used for the production of glucose. Other than fat, glucose is stored in most tissues, as an energy resource ... In humans, these include cytochrome P450 oxidases, UDP-glucuronosyltransferases, and glutathione S-transferases. This system of ... The generation of glucose from compounds like pyruvate, lactate, glycerol, glycerate 3-phosphate and amino acids is called ... Insulin is produced in response to rises in blood glucose levels. Binding of the hormone to insulin receptors on cells then ...
... since they do not possess the gene for L-gulonolactone oxidase, the enzyme required to convert glucose to ascorbic acid. The ...
Several pathways for glucose catabolism are present in its genome, such as the Embden-Meyerhof-Parnas pathway (EMP), the Entner ... R. sphaeroides encodes several terminal oxidases which allow electron transfer to oxygen and other electron acceptors (e.g. ... Tao Y, Liu D, Yan X, Zhou Z, Lee JK, Yang C (2012). "Network Identification and Flux Quantification of Glucose Metabolism in ... Moreover, partitioning of intracellular fluxes has been studied in detail, also with the help of 13C-glucose isotopomers. ...
Many nanomaterials have been used to mimic varieties of natural enzymes, such as oxidase, peroxidase, catalase, SOD, nuclease, ... and glucose oxidation. Metallic nanoparticles can catalyze C-C coupling reactions such as the hydroformylation of olefins, the ...
... (HVA) is a major catecholamine metabolite that is produced by a consecutive action of monoamine oxidase and ... brain and cerebrospinal fluid levels of HVA are measured as a marker of metabolic stress caused by 2-deoxy-D-glucose. HVA ...
"Crosslinking of wheat dough proteins by glucose oxidase and the resulting effects on bread and croissants". Food Chemistry. 89 ...
This catabolic pathway consumes glucose and produces ATP, NADH and pyruvate. A key step for the regulation of glycolysis is an ... Pick FM, McGartoll MA, Bray RC (January 1971). "Reaction of formaldehyde and of methanol with xanthine oxidase". European ...
... and to produce the enzymes polyamine oxidase, phosphogluconate dehydrogenase, and glucose oxidase. The discovery of penicillin ...
... cytochrome C oxidase - cytokine receptor - cytoplasm - cytoplasmic and nuclear receptor - cytosine - cytoskeletal protein - ... glucose - glutamate - glutamate receptor - glutamic acid - glutamine - glycerine - glycine - glycine receptor - glycolipid - ...
Tests consistent throughout Histophilus colonies are oxidase and catalase tests, with oxidase having positive results and ... Histophilus has the ability to ferment glucose, has variable results with lactose and mannitol and are unable to ferment ...
... a hormone responsible for lowering the body's blood glucose (sugar). Blood glucose levels should be checked frequently in the ... Monoamine Oxidase Inhibitors, Clonidine Withdrawal Including but not limited to cocaine use Misuse of over-the-counter ... Hypoglycemia: After the tumor is removed, insulin is no longer inhibited, which can bring the blood glucose dangerously low. ... monoamine oxidase inhibitors, serotonin norepinephrine reuptake inhibitors (SNRI), and methyldopa.[non-primary source needed] ...
Thus cytochrome oxidase, which has two A hemes (heme a and heme a3) in its structure, contains two moles of heme A per mole ... This mechanism is of therapeutic importance: infusion of heme arginate or hematin and glucose can abort attacks of acute ... Caughey, W. S.; Smythe, G. E.; O'Keeffe, D. H.; Maskasky, J. E.; Smith, M. L. (1975). "Heme A of Cytochrome c Oxidase: ... Cytochrome a refers to the heme A in specific combination with membrane protein forming a portion of cytochrome c oxidase. The ...
Multiple polymers with ferrocene side-chains cross-linked with glucose oxidase have shown oxidation activity which results in ... making them useful as glucose biosensors. This sort of activity is not limited to glucose, as other enzymes can be crosslinked ... electrical potential in the presence of glucose, ...
The question of loss of glucose oxidation has been further explored in terms of loss of respiration. Venkobachar et al. found ... Later studies revealed that Ubiquinol oxidase activity ceases first, and the still-active cytochromes reduce the remaining ... proposed the idea that inhibition of glucose oxidation is a major factor in the bacteriocidal nature of chlorine solutions. ... That leaves the question concerning what causes inhibition of glucose oxidation. The discovery that HClO blocks induction of β- ...
The bacteria is able to use glucose to yield gas and is negative for the oxidase test, which are both quite unique ... V. aerogenes can ferment glucose and a few other carbohydrates to yield organic acids. When this bacteria is cultured, the ... Nov., a Facultatively Anaerobic Marine Bacterium That Ferments Glucose with Gas Production." International Journal of ...
It is an example of a biosensor-based test for glucose. This is similar to the glucose oxidase assay, as the glucose in the ... Clinistrip consists of a section that contains glucose oxidase that has been dried onto the paper pad. ... The intensity of the colour change on the pad reflects the amount of glucose present in the urine. v t e (Articles lacking ... Clinistrip, also known as CLINISTIX or the brand name Diastix, are specific clinical sticks for the detection of glucose. ...
Human who have been poisoned show increased levels of serum amylase and glucose as well as elevated urinary diastase levels ... and likely are catalyzed nonspecifically by the same mixed function oxidase. Diazoxon is further broken down by hydrolases in ... the detoxification of diazoxon is processed through the microsomal mixed function oxidase system. Although not fully understood ...
... such as D-amino acid oxidase and uric acid oxidase. However the last enzyme is absent in humans, explaining the disease known ... For example, in baker's yeast (S. cerevisiae), it has been observed that, with good glucose supply, only a few, small ... De Duve and co-workers discovered that peroxisomes contain several oxidases involved in the production of hydrogen peroxide ( ... They also contain approximately 10% of the total activity of two enzymes (Glucose-6-phosphate dehydrogenase and 6- ...
... glucose oxidase, glucose, and nanofibrillated cellulose. The nanoclay is a rheological aid that improves liquid flow, and the ... glucose prevents oxygen inhibition when the material is cured with ultraviolet light. Experimenting with this ink, the team ...
S. hyicus is catalase positive and oxidase negative. It also normally shows DNase activity. Staphylococcus hyicus produces a ... Most strains are capable of fermentation of glucose, fructose, mannose, lactose, and trehalose but not maltose. Differentiation ...
A. phytoseiuli tests negative for the Oxidase reaction, but catalase activity is detectable. Trypticase soy agar (TSA) or ... Cell wall sugars consist of galactose and glucose. The main fatty acids determined in this organism include 12- ... CSC uses glucose-1-phosphate and L-glutamic acid, and its colonies are more yellow in appearance as compared to its ...
For example, it is known that hypoglycaemia (low blood glucose) in an organism produces a release of catecholamines, which ... One notable system involved in metabolic drug interactions is the enzyme system comprising the cytochrome P450 oxidases. ... like glucose or vancomycin), in which case they may or may not have specific and non-specific transporters distributed on the ... trigger compensation mechanisms thereby increasing blood glucose levels. The release of catecholamines also triggers a series ...
NADPH oxidase. Activation of the enzyme leading to H2O2 production. Agmatine sulfate injection can increase food intake with ... hypoglycemic effects are the result of simultaneous modulation of several molecular mechanisms involved in blood glucose ... Demady DR, Jianmongkol S, Vuletich JL, Bender AT, Osawa Y (January 2001). "Agmatine enhances the NADPH oxidase activity of ... An alternative pathway, mainly in peripheral tissues, is by diamine oxidase-catalyzed oxidation into agmatine-aldehyde, which ...
It should not be used in people taking monoamine oxidase inhibitors, nor in people who have certain adrenal cancers, low ... Like other ß2-agonists, dopexamine lowers potassium levels and raises glucose levels, so there is a risk of exacerbating ...
It is thermophilic, xylanolytic, glucose-fermenting, strictly anaerobic and rod-shaped. The type strain of T. hypogea is SEBR ... Yang, Xianqin; Ma, Kesen (2005). "Purification and characterization of an NADH oxidase from extremely thermophilic anaerobic ...
... is hepatically metabolized by aldehyde oxidase; minor metabolism occurs via cytochrome P450 3A4 (CYP3A4). ... August 22, 2007). "Effects of Olanzapine and Ziprasidone on Glucose Tolerance in Healthy Volunteers". Neuropsychopharmacology. ...
... ... 2018). Amperometric Biosensor and Front-End Electronics for Remote Glucose Monitoring by Crosslinked PEDOT-Glucose Oxidase. ... film onto a polyethylene terephthalate/gold electrode followed by immobilization of cross-linked glucose oxidase by ... we show a novel low-cost and flexible biosensing platform for continuous glucose monitoring. The amperometric biosensing system ...
Glucose Oxidase Microplate Assay Kit. Antibodies, Recombinant proteins, ELISA kits, RNAi, cDNA clones, Antibody Array, Luminex ... Products for research , Metabolism assay kits , Assay kits - Intermediary metabolism , Assay kit - Glucose oxidase > Glucose ...
Anti-guinea pig IgG (H+L) antibody [Glucose oxydase] Product code : *lab0405 ...
How can Glucose Oxidase be used? Glucose Oxidase (GO) is used in liquid and powder glucose laboratory reagents, urine test ... BBIs Glucose Oxidase is tried, tested and proven to perform in over 5 billion test strips every year. The high activity and ... colorimetric blood glucose strips and biosensors for blood glucose monitoring. What makes BBI a leading manufacturer of Glucose ... providing sensor and reagent manufacturers with a range of Glucose Oxidase products with proven performance. ...
Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with ... IMMOBILIZATION OF GLUCOSE-OXIDASE - A COMPARISON OF ENTRAPMENT AND COVALENT BONDING. ARICA, MY; Hasırcı, Vasıf Nejat (1993-01- ... GLUCOSE-OXIDASE SANDWICHED BETWEEN PHEMA LAYERS - A CONTINUOUS-FLOW REACTOR APPLICATION. Date. ... Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl ...
Glucose oxidase Puro Pó de Glicose Natural Oxidase CAS 9001-37-0 Enzima de Glicose Oxidase de Grau Alimentício. Rated 0 out of ... Glucose oxidase Fosfolipase Glucose Oxidase Enzimas Pó:10000u/g CAS 9001-37-0. Rated 0 out of 5 ... Glucose oxidase Glucose Oxidase Enzyme For Animal Feed Additives. Rated 0 out of 5 ... Glucose oxidase Glucose Oxidase Enzyme For Bakery Business. Rated 0 out of 5 ...
Glucose oxidase-induced lysis of erythrocytes. Indian Journal of Experimental Biology. 1995 Jan; 33(1): 75-6. ...
Glucose Oxidase, Xylanase Enzyme, Transglutaminase offered by Infinita Biotech Private Limited, Vadodara, Gujarat. ... Pioneers in the industry, we offer glucose oxidase, xylanase enzyme and transglutaminase from India. ... It oxidizing glucose and producing gluconic acid and hydrogen peroxide.. To make Dough stronger and more elastic.. Strengthens ... It is used to stabilize liquid egg- white by converting glucose to D-gluconic acid.. Additional Information: ...
This enzyme is widely used in making biosensors to detect the glucose levels in blood or serum. It is a flavoprotein, which ... Glucose Oxidase. Also known as GOD. This enzyme is widely used in making biosensors to detect the glucose levels in blood or ...
The EnzyChrom Glucose Oxidase Assay Kit is designed for Research Use Only (RUO). Manufactured in USA ... For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. Method: OD570nm, ...
Copy For Citation Altıkatoğlu Yapaöz M. , Elalmış Y. , Arıöz C. , Kuzu H. 4th European Symposium on Biopolymers, İzmir, Turkey, 02 October 2007, pp.75 ...
Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases. / Moon, T. S.; Nielsen, D. R.; Prather ... Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases. AIChE J. 2012;58:2303-2308. ... title = "Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases", ... Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases. ...
We have performed molecular dynamics simulations to obtain an insight on many forms of contact between glucose oxidase dimer ... Molecular effects of encapsulation of glucose oxidase dimer by graphene. In: RSC ADVANCES, 5 (18). pp. 13570-13578. ...
Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. In: Biosensors and ... Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. / Kang, Xinhuang; Wang, ... title = "Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing", ... Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. ...
Glucose Oxidase(GOx) received numerous applicationsin the food industry and clinical fields. Marine fungi extensively ... Glucose Oxidase(GOx) received numerous applicationsin the food industry and clinical fields. Marine fungi extensively ... Isolation and screening of glucose oxidase producing Aspergillus oryzaestrain MF13 from marine source. Abstract. Author(s): ...
Glucose Oxidase By Infinita Biotech Pvt Ltd. Get latest price. It oxidizing glucose and producing gluconic acid and hydrogen ...
... enzymes were combined with glucose, potassium iodide, and ammonium thiocyanate to create an anti-fungal formulation, which ... Stabilized LPO and glucose oxidase (GOx) enzymes were combined with glucose, potassium iodide, and ammonium thiocyanate to ... glucose, lactoperoxidase (LPO, ≥150 U/mg), and glucose oxidase (GOx, 177 U/mg) were obtained from Sigma-Aldrich. ... Glucose Oxidase. Shoot Weight (g). SD 3. Root Weight (g). SD 3. Plant Height (cm). SD 3. ...
The glucose oxidase method to remove the glucose, a type of industrial enzyme, in the egg white can be completely applied to ... After the egg white is desugared by the glucose oxidase method, the Maillard reaction can be effectively prevented from ... Enzymatic desugar is a method of using glucose oxidase to oxidize glucose in egg liquid to gluconic acid to desugar. It is ... 1. Desugaring effect of glucose oxidase method. Enzymatic desugar is a method of using glucose oxidase to oxidize glucose in ...
Glucose; Glucose Oxidase/farmacologia; Glucose Oxidase/uso terapêutico; Glicólise/efeitos dos fármacos; Humanos; Metformina/ ... Tumor metabolism destruction via metformin-based glycolysis inhibition and glucose oxidase-mediated glucose deprivation for ... Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Glucose Oxidase / ... Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Glucose Oxidase / ...
Glucose oxidase: 9001-37-0, Catalase: 9001-05-2 Synonyms: Glucose oxidase: glucose oxidase; beta-D-glucose:oxygen 1- ... Glucose oxidase - Catalase Mixture (eukaryote)-Megazyme试剂盒. 发表于. 2022年1月11日. 由上海金畔生物科技有限公司 ... Glucose oxidase: 1.1.3.4, Catalase: 1.11.1.16 CAS Number: ... Glucose oxidase (12,000 U) plus Catalase (300,000 U) per vial ... High purity Glucose oxidase/Catalase Mixture (eukaryote) for use in research, biochemical enzyme assays and in vitro diagnostic ...
Glucose oxidase. 0 out of 5. $0.00. Read more Add to wishlist ...
The use of B-D(+) glucose-glucose oxidase (E.C. 1.1.3.4)- catalase (E. C. 1. 11. 1. 6) (BDG-GO-CAT) as a new antioxidant system ... Kinetic evaluation and preformulation studies on Beta-D(+) glucose-glucose oxidase-catalase as an antioxidant system in ... Post a Comment for Kinetic evaluation and preformulation studies on Beta-D(+) glucose-glucose oxidase-catalase as an ... Add tags for Kinetic evaluation and preformulation studies on Beta-D(+) glucose-glucose oxidase-catalase as an antioxidant ...
Anzai J, Kobayashi Y, Hoshi T, Saiki H. A layer-by-layer deposition of Concanavalin A and native glucose oxidase to form ... Anzai, J, Kobayashi, Y, Hoshi, T & Saiki, H 1999, A layer-by-layer deposition of Concanavalin A and native glucose oxidase to ... N2 - Glucose oxidase (GOx) can be assembled into multilayer thin films without any chemical modification, by an alternate ... AB - Glucose oxidase (GOx) can be assembled into multilayer thin films without any chemical modification, by an alternate ...
keywords = "1-Butyl-3-methylimidazolium tetrafluoroborate, Electrocatalytic reaction, Glucose oxidase, Glucose oxidase hybrid, ... on the solubility and enzymatic electrocatalytic reaction of glucose oxidase in water/1-butyl-3-methylimidazolium ... the solubility and electrocatalytic properties are compared between native glucose oxidase (GOx) mixed with phenothiazine- ... on the solubility and enzymatic electrocatalytic reaction of glucose oxidase in water/1-butyl-3-methylimidazolium ...
Development of Crack-Free Alumina Sol-gel/Poly(vinyl Alcohol) Membranes for Glucose Oxidase Immobilization
The sensor uses benzoquinone as a mediator for electron transfer between immobilized glucose oxidase and a pyrolytic graphite ... A linear current response, proportional to glucose concentration in the range 1-50mM, is observed. The influence of oxygen,pH ... An amperometric enzyme sensor for the analysis of glucose is described. ...
Enzymes (e.g., horseradish peroxidase and glucose oxidase). [10,40,49,73]. ... Small molecules (e.g., hydrogen peroxide, β-nicotinamide adenine dinucleotide, dopamine and glucose). [11,50,74,75]. ...
... and the oxidation of glucose to gluconic acid by glucose oxidase and catalase. Catalase was added in order to decompose the ... hydrogen peroxide an inhibitor of glucose oxidase formed as by-product of the oxidation. Two processes were employed. Fed-batch ... The best fed-batch performance was attained through the decreasing linear addition of sucrose (64g/L) and glucose (32g/L). ... in which the hydrolysis and oxidation reactions were carried out separately by adding invertase followed by glucose oxidase and ...
Glucose Oxidase from Aspergillus niger. Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen) ... Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in ...
  • The currently used enzyme preparations from the enzyme factory contain not only glucose oxidase, but also catalase, which can decompose hydrogen peroxide in the egg liquid to form oxygen, but hydrogen peroxide needs to be continuously added to the egg liquid to avoid foaming and relieve the suppression of the reaction by the low concentration of the reactant. (bestzyme.com)
  • High purity Glucose oxidase/Catalase Mixture (eukaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (jinpanbio.com)
  • The use of B-D(+) glucose-glucose oxidase (E.C . 1.1.3.4)- catalase (E . C . 1 . (musc.edu)
  • These studies indicate that B-D{+) glucoseglucose oxidase-catalase may be used as an effective antioxidant system in pharmaceutical solution formulations . (musc.edu)
  • Mediante os resultados dos testes com os dois substratos, realizou-se teste de conversão direta da sacarose em frutose e ácido glicônico, utilizando-se invertase, glicose oxidase e catalase em regime descontínuo-alimentado, com alimentação linear decrescente (melhor resultado para ambos os substratos). (usp.br)
  • This work dealt with the hydrolysis of sucrose by invertase into fructose and glucose, and the oxidation of glucose to gluconic acid by glucose oxidase and catalase. (usp.br)
  • Catalase was added in order to decompose the hydrogen peroxide an inhibitor of glucose oxidase formed as by-product of the oxidation. (usp.br)
  • Fed-batch in which the hydrolysis and oxidation reactions were carried out separately by adding invertase followed by glucose oxidase and catalase was conducted by adding the solution of substrate according to a constant, increasing linear, decreasing linear, increasing exponential or decreasing exponential mode. (usp.br)
  • The isolate was motile, catalase positive, oxidase negative, and it fermented glucose and lactose. (cdc.gov)
  • For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. (enzychrome.com)
  • It is also used for the determination of glucose and peroxides in solution. (sigmaaldrich.com)
  • The EnzyChrom Glucose Oxidase Assay Kit is designed for Research Use Only (RUO). (enzychrome.com)
  • For use in removal of excess glucose in conjunction with the Sucrose/Glucose Assay Kit (K-SUCGL) and the Fructan HK Assay Kit (K-FRUCHK). (jinpanbio.com)
  • Enzymatic desugar is a method of using glucose oxidase to oxidize glucose in egg liquid to gluconic acid to desugar. (bestzyme.com)
  • The method can be used to determine the enzymatic activity of the system at glucose oxidase concentrations of 0.005 to 0.030 unit/ml , with an r2 of more than 0.995 for the linearity of the standard curve , and can be adapted for analysis in any pharmaceutical solution . (musc.edu)
  • Imabayashi, SI , Ishii, K & Watanabe, M 2006, ' Effect of the modification of phenothiazine-labeled poly(ethylene oxide) on the solubility and enzymatic electrocatalytic reaction of glucose oxidase in water/1-butyl-3-methylimidazolium tetrafluoroborate mixtures ', Electrochemistry Communications , vol. 8, no. 1, pp. 45-50. (elsevier.com)
  • The enzymatic glucose-oxidase method was employed in glucose estimation. (academicjournals.org)
  • As starch cannot be fermented to ethanol by the traditional fermentation yeast Saccharomyces cerevisiae , the starch in the grains must first be degraded to fermentable sugars, glucose and maltose, by enzymatic degradation before fermentation. (biomedcentral.com)
  • In water/1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF 4 ]) mixtures, the solubility and electrocatalytic properties are compared between native glucose oxidase (GOx) mixed with phenothiazine-labeled poly(ethylene oxide) (PT-PEO) mediator and GOx-(PT-PEO) hybrid, in which PT-PEO is covalently attached to the surface lysine residues, under a substrate-saturated and diffusion-limited condition. (elsevier.com)
  • In this study, matrix entrapment of the enzyme glucose oxidase was achieved through gamma-irradiation of monomers N-vinyl pyrrolidone, 2-hydroxyethyl methacrylate and their mixture. (metu.edu.tr)
  • Oxidative stress in skin of B6C3F1 mice of both sexes was generated by intradermal injection of the hydrogen peroxide (H2O2) -producing enzyme, glucose oxidase with polyethylene glycol (GOD+PEG). (cdc.gov)
  • This is because of the presence of the enzyme glucose oxidase in honey. (traya.health)
  • Fasting and postprandial plasma glucose screening can replace gestational diabetes mellitus screening in women who refuse to have the glucose load test. (who.int)
  • The prevalence of GDM postprandial plasma glucose screening could replace is as high as 9.2%, according to a 2014 analysis ( 2 ). (who.int)
  • Some hours later, the laboratory plasma glucose result (taken at the same time as the Medisense capillary sample) is available. (bestbets.org)
  • plasma glucose: glucose oxidase method). (bestbets.org)
  • Anthropometric parameters (height, weight and waist circumference), blood pressure, fasting plasma glucose, and serum lipids (HDL-cholesterol and triglycerides) were measured. (biomedcentral.com)
  • Mean plasma glucose was found to be highest in the HbSSc state (84.80 ± 4.10 mg/dl), followed by HbSS (78.59 ± 4.20 mg/dl), HbAS (74.80 ± 6.20 mg/dl) and lowest in HbAA (70.10 ± 0.05 mg/dl). (academicjournals.org)
  • The working electrode is made of electropolymerized poly (3,4-ethylenedioxythiophene) film onto a polyethylene terephthalate/gold electrode followed by immobilization of cross-linked glucose oxidase by glutaraldehyde. (cam.ac.uk)
  • Defects and functional groups of the GO are beneficial to immobilization of glucose oxidase (GOD), but sacrificing electron-transfer capability for highly-sensitive detection. (imr.ac.cn)
  • In this work was developed a platform with a conductor material made of silica, titania and graphite carbon to be used as base for immobilization of gold nanoparticles and glucose oxidase enzyme. (ufrgs.br)
  • Immobilization of glucose oxidase and urease in hydrogels of 2-hydroxyethyl methacrylae, and N-vinyl pyrrolidone (NVP) was achieved by irradiation (using UV kand gamma-rays). (metu.edu.tr)
  • It oxidizing glucose and producing gluconic acid and hydrogen peroxide. (infinitabiotech.in)
  • It is used to stabilize liquid egg- white by converting glucose to D-gluconic acid. (infinitabiotech.in)
  • Glucose Oxidase (GO) is used in liquid and powder glucose laboratory reagents, urine test strips, colorimetric blood glucose strips and biosensors for blood glucose monitoring. (bbisolutions.com)
  • This enzyme is widely used in making biosensors to detect the glucose levels in blood or serum. (bioveningredients.com)
  • The biosensors based on glucose oxidase are of great importance for monitoring the glucose in blood, besides helping in diagnostic and treatment of diseases like diabetes. (ufrgs.br)
  • Patients taking any monoamine oxidase inhibitor (MAOI) or within two weeks of taking an MAOI. (ebmconsult.com)
  • This study, part of the Tehran Lipid and Glucose Study, determined and compared the prevalence of overweight and its associated risk factors in 2 555, 1 329 and 1 158 Tehran children and adolescents in 2000, 2003 and 2006 respectively. (who.int)
  • Febuxostat also suppressed NADPH oxidase expressions and enhanced SOD activity in HG-treated GEnCs. (asn-online.org)
  • XO inhibition attenuates HG-induced oxidative stress through the activation of AMPK-PGC-1α-FoxO3a-NADPH oxidase signaling via purine salvage pathway. (asn-online.org)
  • Glucose oxidase method for sugar removal should firstly use 10% organic acid to modulate protein solution (the yolk or whole egg solution does not need to add acid) to make pH reach about 7.0, then add 0.01% to 0.04% glucose oxidase, slowly stir and add at the same time 0.35% of 7% hydrogen peroxide. (bestzyme.com)
  • Honey often contains significant quantities of glucose oxidase, an enzyme that promotes the production of hydrogen peroxide as honey ages. (alive.com)
  • It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (bvsalud.org)
  • Glucose oxidase can break down glucose sugars to produce hydrogen peroxide which can lighten the colour of your hair. (traya.health)
  • Neste trabalho foi desenvolvida uma plataforma com um material condutor a base de sílica, titânia e carbono grafite para servir como matriz de imobilização de nanopartículas de ouro e enzima glicose oxidase. (ufrgs.br)
  • Enzima de la clase de las oxidorreductasas, que cataliza la conversión de beta-D-glucosa y oxígeno a D-glucono-1,5-lactona y peróxido. (bvsalud.org)
  • La enzima es producida por el Penicillium notatum y otros hongos y tiene actividad antibacteriana en presencia de glucosa y oxígeno. (bvsalud.org)
  • Body mass index measurements were taken and blood pressure, fasting blood glucose, cholesterol and triglycerides measured. (who.int)
  • Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. (metu.edu.tr)
  • These are already recommended for reducing the blood pressure control and cholesterol levels of oxidase and burn serum potassium or a way to reduce blood pressure. (centrumlucina.cz)
  • Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl methacrylate). (metu.edu.tr)
  • A linear current response, proportional to glucose concentration in the range 1-50mM, is observed. (ias.ac.in)
  • AlGaN/ GaN High Electron Mobility Transistors (HEMTS) were used to detect pH value and glucose concentration in the exhaled breath condensate (EBC). (tamu.edu)
  • We have performed molecular dynamics simulations to obtain an insight on many forms of contact between glucose oxidase dimer and the single-layer graphene nano-sheets. (iisc.ac.in)
  • Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. (princeton.edu)
  • This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. (princeton.edu)
  • Graphene oxide (GO) has received considerable attention for glucose detection because of high surface area, abundant functional groups, and good biocompatibility. (imr.ac.cn)
  • A graphene-laminated electrode with high glucose oxidase loading for highly-sensitive glucose detection[J]. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,2021,66:57-63. (imr.ac.cn)
  • 2021).A graphene-laminated electrode with high glucose oxidase loading for highly-sensitive glucose detection. (imr.ac.cn)
  • Glucose oxidase (GOx) can be assembled into multilayer thin films without any chemical modification, by an alternate deposition of Concanavalin A (Con A) and native GOx on the surface of electrode. (elsevier.com)
  • The sensor uses benzoquinone as a mediator for electron transfer between immobilized glucose oxidase and a pyrolytic graphite electrode. (ias.ac.in)
  • BestBets: What is the normal range of blood glucose concentrations in healthy term newborns? (bestbets.org)
  • Breast fed full term babies with low blood glucose concentrations produce ketones and other fuels as an adaptive mechanism. (bestbets.org)
  • Sociodemographic and diagnosis variables (presence of dental calculus and periodontal status) were considered to measure salivary concentrations of glucose (by the glucose oxidase/peroxidase method, amylase (by the colorimetric test), urea (by the amount of indophenol), total protein (by the Bradford method) and albumin (by the nephelometric method). (bvsalud.org)
  • Neste estudo, avaliou-se a hidrólise da sacarose pela invertase assim como a conversão da glicose em ácido glicônico, pela ação da glicose oxidase, ambas em processo descontínuo-alimentado. (usp.br)
  • Os biossensores a base de glicose oxidase são de grande importância para monitorar a glicose no sangue, além de auxiliar no diagnóstico e tratamento de doenças como a diabetes. (ufrgs.br)
  • Theoretical suggestion that the metabolism and plasma levels of some hexoses (glucose and fructose) in sickle cell anaemia could be altered was investigated. (academicjournals.org)
  • High blood glucose was defined as a fasting glucose of 126 mg/dL retinopathy, could worsen in Mexico, and the study of screening or more or, if fasting glucose was unavailable, as random glucose systems for diabetic retinopathy is important. (cdc.gov)
  • A capillary heel prick blood test (Medisense) done on the postnatal ward has given a blood glucose reading of 2.6 mmol. (bestbets.org)
  • Range of blood glucose was 1.4 to 5.3 mmol/l. (bestbets.org)
  • The normal range of blood glucose is around 1.5-6 mmol/l in the first days of life, depending on the age of the baby, type of feed, assay method used, and possibly the mode of delivery. (bestbets.org)
  • Up to 14% of healthy term babies may have blood glucose less than 2.6 mmol/l in the first three days of life. (bestbets.org)
  • There is no reason to routinely measure blood glucose in appropriately grown term babies who are otherwise well. (bestbets.org)
  • According to senior author Giuseppe Battaglia, there's a continuing increase in the gradient of glucose within blood vessels into the brain which had helped in guiding their pods through the barrier. (wallstreetpit.com)
  • There was a general improvement in mean values of blood lipids, fasting blood glucose and HbA1c in all the groups, with NEP showing the greatest improvements, followed by NE, except for triglycerides and HDL where the control group had better improvement than the NE. (biomedcentral.com)
  • The value for hypoglycemia is a blood glucose level of less than 50 mg/dL. (medscape.com)
  • as the heart to be a clot or starting meditation of glucose, and why does sepsis lower blood pressure blood pressure control. (centrumlucina.cz)
  • herbal supplements to lower blood sugar diabetes cure diet medical diabetes treatment and drugs Roche 2022 blood sugar balance Ozempic diabetes medications type 2 diabetes medications brands type 2 diabetes drugs side effects normal glucose levels for type 2 diabetes. (smartkitty.eu)
  • In addition, infants were fitted with a device that monitored their blood glucose level continuously, charting their levels every 5 minutes. (smartkitty.eu)
  • Gliclazide increases the release of insulin from the functioning of pancreatic beta cells to lower the blood glucose. (netmeds.com)
  • GLEZNOR M 60 controls blood glucose levels, where gliclazide works by increasing the amount of insulin released by the pancreas in order to lower the blood glucose. (netmeds.com)
  • Concomitant use of CETAL SINUS with tricyclic antidepressants, sympathomimetic agents (such as decongestants, appetite suppressants and amfetamine-like psychostimulants) or with monoamine oxidase inhibitors, which interfere with the catabolism of sympathomimetic amines, may occasionally cause a rise in blood pressure (see Contraindications ). (eipico.com.eg)
  • Hemoglobinuria amongst adult Nigerians as a end result of glucose-6-phosphate dehydrogenase deficiency with drug sensitivity. (edu.hk)
  • Y enterocolitica is non-lactose-fermenting, glucose-fermenting, and oxidase-negative facultative anaerobe that is motile at 25°C and nonmotile at 37°C. Most, but not all, Y enterocolitica isolates reduce nitrates. (medscape.com)
  • It is non-motile, non-sporeforming, and ferments glucose with the production of lactic acid. (quintessay.com)
  • That amount of enzyme causing the oxidation of one micromole of glucose per minute at 25C and pH 7.0. (bbisolutions.com)
  • It is a flavoprotein, which catalyses the oxidation of glucose. (bioveningredients.com)
  • Glucose oxidase was entrapped between poly(2-hydroxyethyl methacrylate) membranes and conditions were optimized for high enzyme activity and high levels of entrapment. (metu.edu.tr)
  • Glucose oxidase was immobilized onto poly(2-hydroxyethyl methacrylate) membranes by covalent bonding through epichlorohydrin. (metu.edu.tr)
  • Glucose oxidase was immobilized onto poly(2-hydroxyethyl methacrylate) (pHEMA) membranes by two methods: by covalent bonding through epichlorohydrin and by entrapment between pHEMA membranes. (metu.edu.tr)
  • Xanthine oxidase (XO) contribute to reactive oxygen species (ROS) production, and XO inhibitors have been reported to the protection of kidney diseases. (asn-online.org)
  • The efficiency of glipizide is likely to be altered by the influence of Monoamine Oxidase Inhibitors (MAOI's), diuretics and cimetidine. (petcarerx.com)
  • Patients who are taking or have taken monoamine oxidase inhibitors within the preceding two weeks. (eipico.com.eg)
  • of note, insulin is not required for the uptake of glucose by the intestinal cells. (medscape.com)
  • Glucose is transported into the cells by an active, energy-requiring process that involves a specific transport protein and requires a concurrent uptake of sodium ions. (medscape.com)
  • Focusing on the interplay between interface chemistry, electrochemistry, and integrated electronics, we show a novel low-cost and flexible biosensing platform for continuous glucose monitoring. (cam.ac.uk)
  • 2-stage oral glucose tolerance test (OGTT) between 24 dL or the 2-hour postprandial or glucose challenge test and 28 weeks of pregnancy ( 6 ). (who.int)
  • The reaction was first order with respect to glucose and to oxygen. (metu.edu.tr)
  • After the egg white is desugared by the glucose oxidase method, the Maillard reaction can be effectively prevented from occurring during dry-heat treatment. (bestzyme.com)
  • Tumor metabolism destruction via metformin-based glycolysis inhibition and glucose oxidase-mediated glucose deprivation for enhanced cancer therapy. (bvsalud.org)
  • Glucose measurements are used in the diagnosis and treatment of pancreatic islet cell carcinoma and of carbohydrate metabolism disorders, including diabetes mellitus, neonatal hypoglycemia, and idiopathic hypoglycemia. (cdc.gov)
  • An amperometric enzyme sensor for the analysis of glucose is described. (ias.ac.in)
  • An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. (bvsalud.org)
  • The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. (bvsalud.org)
  • Pioneers in the industry, we offer glucose oxidase, xylanase enzyme and transglutaminase from India. (infinitabiotech.in)
  • ZnO nanorod-gated AlGaN/GaN HEMTs were used to detect glucose in EBC. (tamu.edu)
  • The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 μA mM -1 cm -2 ) as compared with other nanostructured supports. (princeton.edu)
  • Herein, an intelligent nanomedicine based on glucose deprivation and glycolysis inhibition is creatively constructed for enhanced cancer synergistic treatment . (bvsalud.org)
  • We investigated the cytoprotective mechanism associated with activated AMP-activated protein kinase (AMPK) by XO inhibition in high glucose (HG) condition. (asn-online.org)
  • The optimum pH of glucose oxidase method for sugar removal is 3-8, generally, 6.7-7.2 is the best. (bestzyme.com)
  • The optimum pH and temperature of free and immobilized glucose isomerase were found to be the same values as 7.5 and 60C, respectively. (metu.edu.tr)
  • A ZnO nanorod array was selectively grown on the gate area using low temperature hydrothermal decomposition to immobilize glucose oxidase (GOx). (tamu.edu)