Glucose Oxidase
An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
Enzymes, Immobilized
Glucose
NADPH Oxidase
A flavoprotein enzyme that catalyzes the univalent reduction of OXYGEN using NADPH as an electron donor to create SUPEROXIDE ANION. The enzyme is dependent on a variety of CYTOCHROMES. Defects in the production of superoxide ions by enzymes such as NADPH oxidase result in GRANULOMATOUS DISEASE, CHRONIC.
Biosensing Techniques
Glucose 1-Dehydrogenase
Hydrogen Peroxide
Xanthine Oxidase
Lactoperoxidase
Electrodes
Glucose Dehydrogenases
Glucose Tolerance Test
Penicillium
Monoamine Oxidase
An enzyme that catalyzes the oxidative deamination of naturally occurring monoamines. It is a flavin-containing enzyme that is localized in mitochondrial membranes, whether in nerve terminals, the liver, or other organs. Monoamine oxidase is important in regulating the metabolic degradation of catecholamines and serotonin in neural or target tissues. Hepatic monoamine oxidase has a crucial defensive role in inactivating circulating monoamines or those, such as tyramine, that originate in the gut and are absorbed into the portal circulation. (From Goodman and Gilman's, The Pharmacological Basis of Therapeutics, 8th ed, p415) EC 1.4.3.4.
Electrochemistry
Protein-Lysine 6-Oxidase
Catalase
Oxidation-Reduction
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Glucose Intolerance
A pathological state in which BLOOD GLUCOSE level is less than approximately 140 mg/100 ml of PLASMA at fasting, and above approximately 200 mg/100 ml plasma at 30-, 60-, or 90-minute during a GLUCOSE TOLERANCE TEST. This condition is seen frequently in DIABETES MELLITUS, but also occurs with other diseases and MALNUTRITION.
Aldehyde Oxidase
An aldehyde oxidoreductase expressed predominantly in the LIVER; LUNGS; and KIDNEY. It catalyzes the oxidation of a variety of organic aldehydes and N-heterocyclic compounds to CARBOXYLIC ACIDS, and also oxidizes quinoline and pyridine derivatives. The enzyme utilizes molybdenum cofactor and FAD as cofactors.
Polarography
An electrochemical technique for measuring the current that flows in solution as a function of an applied voltage. The observed polarographic wave, resulting from the electrochemical response, depends on the way voltage is applied (linear sweep or differential pulse) and the type of electrode used. Usually a mercury drop electrode is used.
Oxidoreductases
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Reagent Strips
Flavin-Adenine Dinucleotide
Glycosuria
Horseradish Peroxidase
Indicators and Reagents
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Hexokinase
An enzyme that catalyzes the conversion of ATP and a D-hexose to ADP and a D-hexose 6-phosphate. D-Glucose, D-mannose, D-fructose, sorbitol, and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase. (From Enzyme Nomenclature, 1992) EC 2.7.1.1.
Glucose Transporter Type 1
Platinum
Platinum. A heavy, soft, whitish metal, resembling tin, atomic number 78, atomic weight 195.09, symbol Pt. (From Dorland, 28th ed) It is used in manufacturing equipment for laboratory and industrial use. It occurs as a black powder (platinum black) and as a spongy substance (spongy platinum) and may have been known in Pliny's time as "alutiae".
Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals. (1/420)
Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a reporter system based on the Aspergillus niger glucose oxidase. Strains harboring fusions of the ech42 or nag1 5' upstream noncoding sequences with the A. niger goxA gene displayed a glucose oxidase activity pattern that was consistent under various conditions with expression of the native ech42 and nag1 genes, as assayed by Northern analysis. The expression product of goxA in the mutants was completely secreted into the medium, detectable on Western blots, and quantifiable by enzyme-linked immunosorbent assay. nag1 gene expression was triggered during growth on fungal (Botrytis cinerea) cell walls and on the chitin degradation product N-acetylglucosamine. N-Acetylglucosamine, di-N-acetylchitobiose, or tri-N-acetylchitotriose also induced nag1 gene expression when added to mycelia pregrown on different carbon sources. ech42 expression was also observed during growth on fungal cell walls but, in contrast, was not triggered by addition of chitooligomers to pregrown mycelia. Significant ech42 expression was observed after prolonged carbon starvation, independent of the use of glucose or glycerol as a carbon source, suggesting that relief of carbon catabolite repression was not involved in induction during starvation. In addition, ech42 gene transcription was triggered by physiological stress, such as low temperature, high osmotic pressure, or the addition of ethanol. Four copies of a putative stress response element (CCCCT) were found in the ech42 promoter. (+info)Strictures in Crohn's disease are characterised by an accumulation of mast cells colocalised with laminin but not with fibronectin or vitronectin. (2/420)
BACKGROUND/AIMS: Intestinal fibrosis and stricture formation is an unresolved problem in Crohn's disease. The aim of this study was to investigate whether mast cells accumulate in these tissues and whether their localisation is associated with extracellular matrix components. METHODS: Mast cells were visualised by immunohistochemical staining of the mast cell specific proteases chymase and tryptase. Their localisation in relation to extracellular matrix components was shown by immunohistochemical double labelling. RESULTS: In strictures in Crohn's disease, a striking accumulation of mast cells was seen particularly in the hypertrophied and fibrotic muscularis propria, with a mean (SEM) mast cell number of 81.3 (14.9) v 1.5 (0.9)/mm(2) in normal bowel (p<0.0005). All mast cells in the muscularis propria were colocalised with patches of laminin. In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. No colocalisation of mast cells was found with fibronectin or vitronectin. CONCLUSIONS: The large accumulation of mast cells in the muscle layer of strictured bowel suggests a functional role for these cells in the hypertrophic and fibrotic response of the smooth muscle cells. The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis. (+info)Hydrogen peroxide production from reactive liposomes encapsulating enzymes. (3/420)
Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated in combination with horseradish peroxidase (HRP) or lactoperoxidase (LPO). The generation of hydrogen peroxide from the liposomes in response to externally added D-glucose substrate was monitored using a Rank electrode system polarised to +650 mV, relative to a standard silver-silver chloride electrode. The effects of encapsulated enzyme concentration, enzyme combinations (GO+HRP, GO+LPO), substrate concentration, electron donor and temperature on the production of hydrogen peroxide have been investigated. The electrode signal (peroxide production) was found to increase linearly with GO incorporation, was reduced on addition of HRP and an electron donor (o-dianisidine) and showed a maximum at the lipid chain-melting temperature from the anionic liposomes containing no cholesterol. To aid interpretation of the results, the permeability of the non-reactive substrate (methyl glucoside) across the bilayer membranes was measured. It was found that the encapsulation of the enzymes effected the permeability coefficients of methyl glucoside, increasing them in the case of anionic liposomes and decreasing them in the case of cationic liposomes. These observations are discussed in terms of enzyme bilayer interactions. (+info)Immunotargeting of glucose oxidase: intracellular production of H(2)O(2) and endothelial oxidative stress. (4/420)
Extracellular and intracellular reactive oxygen species attack different targets and may, therefore, result in different forms of oxidative stress. To specifically study an oxidative stress induced by a regulated intracellular flux of a defined reactive oxygen species in endothelium, we used immunotargeting of the H(2)O(2)-generating enzyme glucose oxidase (GOX) conjugated with an antibody to platelet-endothelial cell adhesion molecule (PECAM)-1, an endothelial surface antigen. Anti-PECAM-(125)I-GOX conjugates specifically bind to both endothelial and PECAM-transfected cells. Approximately 70% of cell-bound anti-PECAM-(125)I-GOX was internalized. The cell-bound conjugate was enzymatically active and generated H(2)O(2) from glucose. Use of the fluorescent dye dihydrorhodamine 123 revealed that 70% of H(2)O(2) was generated intracellularly, whereas 30% of H(2)O(2) was detected in the cell medium. Catalase added to the cells eliminated H(2)O(2) in the medium but had little effect on the intracellular generation of H(2)O(2) by anti-PECAM-GOX. Both H(2)O(2) added exogenously to the cell medium (extracellular H(2)O(2)) and that generated by anti-PECAM-GOX caused oxidative stress manifested by time- and dose-dependent irreversible plasma membrane damage. Inactivation of cellular catalase by aminotriazole treatment augmented damage caused by either extracellular H(2)O(2) or anti-PECAM-GOX. Catalase added to the medium protected either normal or aminotriazole-treated cells against extracellular H(2)O(2), yet failed to protect cells against injury induced by anti-PECAM-GOX. Therefore, treatment of PECAM-positive cells with anti-PECAM-GOX leads to conjugate internalization, predominantly intracellular H(2)O(2) generation and intracellular oxidative stress. These results indicate that anti-PECAM-GOX 1) provides cell-specific intracellular delivery of an active enzyme and 2) causes intracellular oxidative stress in PECAM-positive cells. (+info)Expression of amylase and glucose oxidase in the hypopharyngeal gland with an age-dependent role change of the worker honeybee (Apis mellifera L.). (5/420)
Worker honeybees change their behaviour from the role of nurse to that of forager with age. We have isolated cDNA clones for two honeybee (Apis mellifera L.) genes, encoding alpha-amylase and glucose oxidase homologues, that are expressed in the hypopharyngeal gland of forager bees. The predicted amino acid sequence of the putative Apis amylase showed 60.5% identity with Drosophila melanogaster alpha-amylase, whereas that of Apis glucose oxidase showed 23.8% identity with Aspergillus niger glucose oxidase. To determine whether the isolated cDNAs actually encode these enzymes, we purified amylase and glucose oxidase from homogenized forager-bee hypopharyngeal glands. We sequenced the N-terminal regions of the purified enzymes and found that they matched the corresponding cDNAs. mRNAs for both enzymes were detected by Northern blotting in the hypopharyngeal gland of the forager bee but not in the nurse-bee gland. These results clearly indicate that expression of the genes for these carbohydrate-metabolizing enzymes, which are needed to process nectar into honey, in the hypopharyngeal gland is associated with the age-dependent role change of the worker. (+info)Second harmonic generation of glucose oxidase at the air/water interface. (6/420)
We present a study of the adsorption of the glucose oxidase enzyme (GOx) at the air/water interface, using the nonlinear optical technique of surface second harmonic generation (SSHG). Resonant SSHG experiments were achieved by probing the pi-pi* transition of the flavin adenine dinucleotide (FAD) chromophores embedded in the GOx protein. Because of the subsequent resonance enhancement of the signal, the second harmonic (SH) wave arising from the GOx entities adsorbed at the interface was detectable for protein bulk aqueous concentrations as low as 70 nM. The protein adsorption was followed, and, at high GOx coverage, a change in the orientation of the FAD chromophore was observed, indicating either a rearrangement or a reorientation of the protein at the interface. Inasmuch as GOx is negatively charged at the biological pH of 7, its interactions with charged surfactants were also investigated. As expected, spreading positively charged surfactants onto a partial protein monolayer was found to increase the GOx surface concentration, whereas in the case of negatively charged surfactants, the GOx surface concentration decreased until the SH signal went back to the pure buffer solution response level. With the increasing GOx surface concentration, the rearrangement or reorientation of the protein was also observed. (+info)Imaging of biological macromolecules on mica in humid air by scanning electrochemical microscopy. (7/420)
Imaging of DNA, keyhole limpet hemocyanin, mouse monoclonal IgG, and glucose oxidase on a mica substrate has been accomplished by scanning electrochemical microscopy with a tungsten tip. The technique requires the use of a high relative humidity to form a thin film of water on the mica surface that allows electrochemical reactions to take place at the tip and produce a faradaic current (approximately 1 pA) that can be used to control tip position. The effect of relative humidity and surface pretreatment with buffer solutions on the ionic conductivity of a mica surface was investigated to find appropriate conditions for imaging. Resolution of the order of 1 nm was obtained. (+info)Conserved arginine-516 of Penicillium amagasakiense glucose oxidase is essential for the efficient binding of beta-D-glucose. (8/420)
The effects of mutation of key conserved active-site residues (Tyr-73, Phe-418, Trp-430, Arg-516, Asn-518, His-520 and His-563) of glucose oxidase from Penicillium amagasakiense on substrate binding were investigated. Kinetic studies on the oxidation of beta-D-glucose combined with molecular modelling showed the side chain of Arg-516, which forms two hydrogen bonds with the 3-OH group of beta-D-glucose, to be absolutely essential for the efficient binding of beta-D-glucose. The R516K variant, whose side chain forms only one hydrogen bond with the 3-OH group of beta-D-glucose, exhibits an 80-fold higher apparent K(m) (513 mM) but a V(max) only 70% lower (280 units/mg) than the wild type. The complete elimination of a hydrogen-bond interaction between residue 516 and the 3-OH group of beta-D-glucose through the substitution R516Q effected a 120-fold increase in the apparent K(m) for glucose (to 733 mM) and a decrease in the V(max) to 1/30 (33 units/mg). None of the other substitutions, with the exception of variant F418A, affected the apparent K(m) more than 6-fold. In contrast, the removal of aromatic or bulky residues at positions 73, 418 or 430 resulted in decreases in the maximum rates of glucose oxidation to less than 1/90. Variants of the potentially catalytically active His-520 and His-563 were completely, or almost completely, inactive. Thus, of the residues forming the active site of glucose oxidase, Arg-516 is the most critical amino acid for the efficient binding of beta-D-glucose by the enzyme, whereas aromatic residues at positions 73, 418 and 430 are important for the correct orientation and maximal velocity of glucose oxidation. (+info)
Design of Amperometric Biosensors for the Detection of Glucose Prepared by Immobilization of Glucose Oxidase on Conducting ...
Fabrication and characterization of polypyrrole/glucose oxidase electrode on a microfabricated capillary electrophoresis chip ...
Repositorio da Producao Cientifica e Intelectual da Unicamp: Interaction Between Xylanase, Glucose Oxidase And Ascorbic Acid On...
What are the advantages and disadvantages of the glucose oxidase method used for the measurement of glucose concentration in...
Global Glucose Oxidase Market - Medical Market Report
Immobilization of Glucose oxidase on Meso-porous Glass-ceramic with the Skeleton of CaTi4(PO¬4)6
Effects of Wheat Flour Dough’s Viscoelastic Level by Adding Glucose Oxidase on its Dynamic Shear Properties whatever the...
Direct Electrochemistry and Immobilization of Glucose Oxidase on Nano-Carbon Aerogels
Kinetic limitations of a bioelectrochemical electrode using carbon nanotube-attached glucose oxidase for biofuel cells<...
A facile and effective immobilization of glucose oxidase on tannic acid modified CoFe2O4 magnetic nanoparticles
Guanidinium Chloride- and Urea-Induced Unfolding of the Dimeric Enzyme Glucose Oxidase
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Biological Effects of Potato Plants Transformation with Glucose Oxidase Gene and their Resistance to Hyperthermia - CNKI学术搜索
Simple Method for Preparing Glucose Biosensor Based on Glucose Oxidase in Nanocomposite Material of Single-Wall Carbon...
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Patent US5174291 - Process for using a measuring cell assembly for glucose determination - Google Patents
Ceramide- and Oxidant-Induced Insulin Resistance Involve Loss of Insulin-Dependent Rac-Activation and Actin Remodeling in...
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Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold:...
Glucose Oxidase
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BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Chemiluminescence flow biosensor for glucose based on gold nanoparticle-enhanced activities of glucose oxidase and horseradish...
Cloning and Heterologous Expression of Glucose Oxidase Gene from Aspergillus niger Z-25 in Pichia pastoris - DTU Orbit
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RCSB PDB
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In vivo brain glucose measurements: differential normal pulse...
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Entrifuged again, and the two supernatants were mixed together. The extract | http://adenosine-kinase.com
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Glucose oxidase
In the glucose oxidase assay, the glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen ... The glucose oxidase binds specifically to β-D-glucopyranose and does not act on α-D-glucose. It oxidises all of the glucose in ... "Glucose Oxidase: A much used and much loved enzyme in biosensors" at University of Paisley Glucose+Oxidase at the US National ... Glucose oxidase catalyzes the oxidation of β-D-glucose into D-glucono-1,5-lactone, which then hydrolyzes into gluconic acid. In ...
Detlev Müller
The factor was glucose oxidase. In the presence of glucose, the glucose oxidase produced hydrogen peroxide, which killed off ... "Detlev Müller discovers glucose oxidase". Tacomed.com. Archived from the original on 18 April 2018. Retrieved 13 June 2017. ... He is best known for discovering the enzyme glucose oxidase. In 1928, he was experimenting with the common fungus, Aspergillus ... He eventually found that these bacteria could only thrive adjacent to Aspergillus niger if glucose was not present. He ...
Nanoreactor
... trapping the enzyme glucose oxidase. The surrounding solution would end up containing the enzyme lipase B with the glucose ... The resulting glucose would cross the membrane, become oxidized, and the horseradish peroxidase would convert the sample ...
Cladosporium oxysporum
In a study by Oxenbøll et al., C. oxysporum catalyzed glucose oxidation by producing glucose oxidase, which it is mentioned in ... that the glucose oxidase helped protect against bacterial infection on the surface of fungi. The organism is also proven to be ... Oxenbøll, K; Si, J. Q; Aagaard, J (3 May 1995). "Alkaline glucose oxidase obtained from Cladosporium oxysporum". US Grant. ... It breaks down cellulose from dead herbaceous or plants, which is then further turned into glucose to Be used by the fungi ...
Penicillium adametzii
"Thermal stability of Penicillium adametzii glucose oxidase". Prikladnaia biokhimiia i mikrobiologiia. 37 (6): 678-86. PMID ...
Glucose meter
Many glucose meters employ the oxidation of glucose to gluconolactone catalyzed by glucose oxidase (sometimes known as GOx). ... Glucose vs. plasma glucose: Glucose levels in plasma (one of the components of blood) are higher than glucose measurements in ... The glucose in the blood reacts with an enzyme electrode containing glucose oxidase (or dehydrogenase). The enzyme is ... This is important because home blood glucose meters measure the glucose in whole blood while most lab tests measure the glucose ...
Quentin Gibson
Other work concerned enzymes such as "diaphorase", glucose oxidase, cytochrome oxidase and peroxidase. Much of Gibson's work ... Gibson, Q H; Massey, V; Swoboda, B E P J (1964). "Kinetics and mechanism of action of glucose oxidase". J. Biol. Chem. 239 (11 ... Gibson, Q H; Greenwood, C; Wharton, D C; Palmer, G (1965). "Reaction of cytochrome oxidase with cytochrome C". J. Biol. Chem. ... Brunori, M.; Gibson, Q.H. (1983). "Oxygen activation by cytochrome oxidase: A new spectral intermediate observed by flow-flash ...
Liane Marcia Rossi
"Glucose oxidase-magnetite nanoparticle bioconjugate for glucose sensing". Analytical and Bioanalytical Chemistry. 380 (4): 606- ...
Aspergillus niger
... glucose oxidase (P13006), used in the design of glucose biosensors, due to its high affinity for β-D-glucose. In the food ... "Nano-Encapsulation of Glucose Oxidase Dimer by Graphene". RSC Advances. 5 (18): 13570-78. doi:10.1039/C4RA16852F. S2CID ... Staiano M, Bazzicalupo P, Rossi M, D'Auria S (December 2005). "Glucose biosensors as models for the development of advanced ...
Biosensor
Commercially available glucose monitors rely on amperometric sensing of glucose by means of glucose oxidase, which oxidises ... which uses the enzyme glucose oxidase to break blood glucose down. In doing so it first oxidizes glucose and uses two electrons ... "Nano-Encapsulation of Glucose Oxidase Dimer by Graphene". RSC Advances. 5 (18): 13570-78. doi:10.1039/C4RA16852F. Daaboul, G.G ... Measured glucose data will be transmitted wirelessly out of the body within the MICS 402-405 MHz band as approved for medical ...
Pyranose oxidase
Recently, pyranose oxidase has been gaining on popularity within biosensors. Unlike glucose oxidase, it can produce higher ... Other names in common use include glucose 2-oxidase, and pyranose-2-oxidase. This enzyme participates in pentose phosphate ... In enzymology, a pyranose oxidase (EC 1.1.3.10) is an enzyme that catalyzes the chemical reaction D-glucose + O2 ⇌ {\ ... whereas its two products are 2-dehydro-D-glucose and H2O2. Pyranose oxidase is able to oxidize D-xylose, L-sorbose, D-galactose ...
Colorimetric analysis
CHOD = cholesterol oxidase GOD = glucose oxidase GPO = glycerol-3-phosphate oxidase PAP = phenol + aminophenazone (in some ... water Glucose + oxygen + water --(enzyme glucose oxidase)--> gluconate + hydrogen peroxide Hydrogen peroxide + ABTS --(enzyme ... Cholesterol + oxygen --(enzyme cholesterol oxidase)--> cholestenone + hydrogen peroxide Hydrogen peroxide + 4-aminophenazone + ... enzyme glycerol-3-phosphate oxidase) --> dihydroxyacetone phosphate + hydrogen peroxide Hydrogen peroxide + 4-aminophenazone + ...
Artificial enzyme
Glucose oxidase-like gold nanoparticles combined with cyclodextran were used for chiral catalysis. An artificial binuclear ... Glucose oxidase and iron oxide nanozymes were encapsulated within multi-compartmental hydrogels for incompatible tandem ... A Pd12 coordination cage as a photoregulated oxidase-like nanozyme was developed. An NADPH oxidase-like nanozyme was developed ... with glucose oxidase (GOx) in sub-micron proximity, providing a fast and efficient enzyme cascade reported as monitoring ...
Sequence saturation mutagenesis
"Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics". Biosens. Bioelectron. 50: 84-90. ... glucose oxidase for analytical application, phytase with increased thermostability and monooxygenase with improved catalytic ...
Biomolecular engineering
For example , the immobilization of glucose oxidase on calcium alginate gel beads can be used in a bioreactor. The resulting ... Examples include entrapment of enzymes such as glucose oxidase in gel column for use as a bioreactor. Important characteristic ... "Production of gluconic acid with immobilized glucose oxidase in airlift reactors". Chemical Engineering Science. 52 (21-22): ... Kinetic studies of Immobilized glucose oxidase". Biotechnology and Bioengineering. 16 (2): 159-168. doi:10.1002/bit.260160202. ...
Trigona fuscipennis
... bees use the enzyme glucose oxidase, stored in their hypopharyngeal glands. Glucose oxidase works to ... Burgett, D. Michael (January 9, 1974). "Glucose Oxidase: A Food Protective Mechanism in Social Hymenoptera" (PDF). Annals of ...
Cross-linked enzyme aggregate
Biosensors/diagnostics, e.g. glucose oxidase and cholesterol oxidase biosensors. Delivery of proteins as therapeutic agents or ...
Biotene
The three primary enzymes in Biotène are[were? no longer, per the above] Glucose Oxidase, Lactoperoxidase, and Lysozyme. ... and glucose oxidase. The PBF products have been discontinued. People with xerostomia (dry mouth) may use Biotène to reduce the ... and prior to the GlaxoSmithKline acquisition it also contained enzymes including glucose oxidase, lactoferrin, lactoperoxidase ...
Clark electrode
Now, however, the membrane is impregnated with immobilized glucose oxidase (GOx). The GOx will consume some of the oxygen as it ... leaving as the only variable the oxygen and glucose concentrations on the analyte-side of the glucose membrane, which is the ... The Clark oxygen electrode laid the basis for the first glucose biosensor (in fact the first biosensor of any type), invented ... The rate of reaction current is limited by the diffusion of both glucose and oxygen. This diffusion can be well characterized ...
Colloidal gold
GNP-glucose oxidase monolayer electrode was constructed use these two methods. The Au NP allowed more freedom in the enzyme's ... These ribbons are very flexible and can bend with angles larger than 90°. When glucose is replaced by cyclodextrin (a glucose ... In another method using ultrasound, the reaction of an aqueous solution of HAuCl4 with glucose, the reducing agents are ... Large AuNPs conjugated with biotin, cysteine, citrate, and glucose were not toxic in human leukemia cells (K562) for ...
Penicillium variabile
Petruccioli, M.; Federici, F. (1993). "Glucose oxidase production by Penicillium variabile P16: Effect of medium composition". ... "Expression of Penicillium variabile P16 glucose oxidase gene in Pichia pastoris and characterization of the recombinant enzyme ...
Self-healing hydrogels
These glucose sensitive hydrogels are modified with the enzyme glucose oxidase. In the presence of glucose, the glucose oxidase ... An example of this would be hydrogels that release insulin in the presence of high glucose levels in the bloodstream. ...
Joseph Kost
"Glucose-sensitive membranes containing glucose oxidase: Activity, swelling, and permeability studies". Journal of Biomedical ... The major focus has been the development of a non-invasive continuous detection of glucose. The first clinical study on ... Since, Kost also proposed a novel approach for a glucose flux continuous biosensor and noninvasive detection of amniotic fluid ... In addition to his studies on responsive controlled release systems: pH sensitive, glucose sensitive and calcium sensitive drug ...
Scanning electrochemical microscopy
Studies of Enzyme-Mediator Kinetics for Membrane and Surface Immobilized Glucose Oxidase". Anal. Chem. 64 (17): 1795-1804. doi: ...
Vinod Bhakuni
... and urea-induced unfolding of the dimeric enzyme glucose oxidase". Biochemistry. 41 (11): 3819-27. doi:10.1021/bi0116700. PMID ...
Sikkim tea
The extract has also shown significant inhibition against glucose oxidase-mediated inflammation. The observations have ...
Oxidase test
Calibration of glucose oxidase-based test strips for capillary blood measurement with oxygen saturated venous blood samples. ... Oxidase-positive bacteria possess cytochrome oxidase or indophenol oxidase (an iron-containing hemoprotein). These both ... The oxidase test is used to determine if an organism possesses the cytochrome c oxidase enzyme. The test is used as an aid for ... Oxidase Test Protocol - Library. American Society for Microbiology, ASM MicrobeLibrary, 1-5. Steel K J. 1961. The Oxidase ...
Adam Heller
2. Methods for bonding electron-transfer relays to glucose oxidase and D-amino-acid oxidase. J. Am. Chem. Soc. Vol. 110. pp. ... Using electrically wired glucose oxidase he and his team engineered prototypes of subcutaneously implanted continuously glucose ... "Design and Optimization of a Selective Subcutaneously Implantable Glucose Electrode Based on "Wired" Glucose Oxidase". ... Amperometric glucose microelectrodes prepared through immobilization of glucose oxidase in redox hydrogels. Anal. Chem. Vol. 63 ...
Sarcosine dehydrogenase
Roth JP, Klinman JP (January 2003). "Catalysis of electron transfer during activation of O2 by the flavoprotein glucose oxidase ... Leys D, Basran J, Scrutton NS (August 2003). "Channelling and formation of 'active' formaldehyde in dimethylglycine oxidase". ...
Penicillium amagasakiense
The Glucose oxidase of Penicillium amagasakiense has been studied in detail because of the use of Glucose oxidase in biosensors ... Zoldak, G.; Zubrik, A.; Musatov, A.; Stupak, M.; Sedlak, E. (2004). "Irreversible Thermal Denaturation of Glucose Oxidase from ... MycoBank ATCC UniProt Conserved arginine-516 of Penicillium amagasakiense glucose oxidase isessential for the efficient binding ... Courjean, O.; Mano, N. (2011). "Recombinant glucose oxidase from Penicillium amagasakiense for efficient bioelectrochemical ...
List of food additives
... acidity regulator Glucose oxidase - antioxidant Glucose syrup - sweetener Glutamate - Glutamic acid - flavor enhancer Gluten - ...
Prostaglandin-endoperoxide synthase 2
Legan M (August 2010). "Cyclooxygenase-2, p53 and glucose transporter-1 as predictors of malignancy in the development of ... PTGS (COX, which can be confused with "cytochrome oxidase") enzymes are monotopic membrane proteins; the membrane-binding ...
Metabolism
... where it can be used for the production of glucose. Other than fat, glucose is stored in most tissues, as an energy resource ... In humans, these include cytochrome P450 oxidases, UDP-glucuronosyltransferases, and glutathione S-transferases. This system of ... The generation of glucose from compounds like pyruvate, lactate, glycerol, glycerate 3-phosphate and amino acids is called ... Insulin is produced in response to rises in blood glucose levels. Binding of the hormone to insulin receptors on cells then ...
Ficus yoponensis
... since they do not possess the gene for L-gulonolactone oxidase, the enzyme required to convert glucose to ascorbic acid. The ...
Rhodobacter sphaeroides
Several pathways for glucose catabolism are present in its genome, such as the Embden-Meyerhof-Parnas pathway (EMP), the Entner ... R. sphaeroides encodes several terminal oxidases which allow electron transfer to oxygen and other electron acceptors (e.g. ... Tao Y, Liu D, Yan X, Zhou Z, Lee JK, Yang C (2012). "Network Identification and Flux Quantification of Glucose Metabolism in ... Moreover, partitioning of intracellular fluxes has been studied in detail, also with the help of 13C-glucose isotopomers. ...
Nanomaterial-based catalyst
Many nanomaterials have been used to mimic varieties of natural enzymes, such as oxidase, peroxidase, catalase, SOD, nuclease, ... and glucose oxidation. Metallic nanoparticles can catalyze C-C coupling reactions such as the hydroformylation of olefins, the ...
Homovanillic acid
... (HVA) is a major catecholamine metabolite that is produced by a consecutive action of monoamine oxidase and ... brain and cerebrospinal fluid levels of HVA are measured as a marker of metabolic stress caused by 2-deoxy-D-glucose. HVA ...
Juliet Gerrard
"Crosslinking of wheat dough proteins by glucose oxidase and the resulting effects on bread and croissants". Food Chemistry. 89 ...
Enzyme inhibitor
This catabolic pathway consumes glucose and produces ATP, NADH and pyruvate. A key step for the regulation of glycolysis is an ... Pick FM, McGartoll MA, Bray RC (January 1971). "Reaction of formaldehyde and of methanol with xanthine oxidase". European ...
Penicillium chrysogenum
... and to produce the enzymes polyamine oxidase, phosphogluconate dehydrogenase, and glucose oxidase. The discovery of penicillin ...
Index of biochemistry articles
... cytochrome C oxidase - cytokine receptor - cytoplasm - cytoplasmic and nuclear receptor - cytosine - cytoskeletal protein - ... glucose - glutamate - glutamate receptor - glutamic acid - glutamine - glycerine - glycine - glycine receptor - glycolipid - ...
Histophilus somni
Tests consistent throughout Histophilus colonies are oxidase and catalase tests, with oxidase having positive results and ... Histophilus has the ability to ferment glucose, has variable results with lactose and mannitol and are unable to ferment ...
Pheochromocytoma
... a hormone responsible for lowering the body's blood glucose (sugar). Blood glucose levels should be checked frequently in the ... Monoamine Oxidase Inhibitors, Clonidine Withdrawal Including but not limited to cocaine use Misuse of over-the-counter ... Hypoglycemia: After the tumor is removed, insulin is no longer inhibited, which can bring the blood glucose dangerously low. ... monoamine oxidase inhibitors, serotonin norepinephrine reuptake inhibitors (SNRI), and methyldopa.[non-primary source needed] ...
Heme
Thus cytochrome oxidase, which has two A hemes (heme a and heme a3) in its structure, contains two moles of heme A per mole ... This mechanism is of therapeutic importance: infusion of heme arginate or hematin and glucose can abort attacks of acute ... Caughey, W. S.; Smythe, G. E.; O'Keeffe, D. H.; Maskasky, J. E.; Smith, M. L. (1975). "Heme A of Cytochrome c Oxidase: ... Cytochrome a refers to the heme A in specific combination with membrane protein forming a portion of cytochrome c oxidase. The ...
Smart inorganic polymer
Multiple polymers with ferrocene side-chains cross-linked with glucose oxidase have shown oxidation activity which results in ... making them useful as glucose biosensors. This sort of activity is not limited to glucose, as other enzymes can be crosslinked ... electrical potential in the presence of glucose, ...
Hypochlorous acid
The question of loss of glucose oxidation has been further explored in terms of loss of respiration. Venkobachar et al. found ... Later studies revealed that Ubiquinol oxidase activity ceases first, and the still-active cytochromes reduce the remaining ... proposed the idea that inhibition of glucose oxidation is a major factor in the bacteriocidal nature of chlorine solutions. ... That leaves the question concerning what causes inhibition of glucose oxidation. The discovery that HClO blocks induction of β- ...
Vibrio aerogenes
The bacteria is able to use glucose to yield gas and is negative for the oxidase test, which are both quite unique ... V. aerogenes can ferment glucose and a few other carbohydrates to yield organic acids. When this bacteria is cultured, the ... Nov., a Facultatively Anaerobic Marine Bacterium That Ferments Glucose with Gas Production." International Journal of ...
Clinistrip
It is an example of a biosensor-based test for glucose. This is similar to the glucose oxidase assay, as the glucose in the ... Clinistrip consists of a section that contains glucose oxidase that has been dried onto the paper pad. ... The intensity of the colour change on the pad reflects the amount of glucose present in the urine. v t e (Articles lacking ... Clinistrip, also known as CLINISTIX or the brand name Diastix, are specific clinical sticks for the detection of glucose. ...
Diazinon
Human who have been poisoned show increased levels of serum amylase and glucose as well as elevated urinary diastase levels ... and likely are catalyzed nonspecifically by the same mixed function oxidase. Diazoxon is further broken down by hydrolases in ... the detoxification of diazoxon is processed through the microsomal mixed function oxidase system. Although not fully understood ...
Peroxisome
... such as D-amino acid oxidase and uric acid oxidase. However the last enzyme is absent in humans, explaining the disease known ... For example, in baker's yeast (S. cerevisiae), it has been observed that, with good glucose supply, only a few, small ... De Duve and co-workers discovered that peroxisomes contain several oxidases involved in the production of hydrogen peroxide ( ... They also contain approximately 10% of the total activity of two enzymes (Glucose-6-phosphate dehydrogenase and 6- ...
4D printing
... glucose oxidase, glucose, and nanofibrillated cellulose. The nanoclay is a rheological aid that improves liquid flow, and the ... glucose prevents oxygen inhibition when the material is cured with ultraviolet light. Experimenting with this ink, the team ...
Staphylococcus hyicus
S. hyicus is catalase positive and oxidase negative. It also normally shows DNase activity. Staphylococcus hyicus produces a ... Most strains are capable of fermentation of glucose, fructose, mannose, lactose, and trehalose but not maltose. Differentiation ...
Acaricomes phytoseiuli
A. phytoseiuli tests negative for the Oxidase reaction, but catalase activity is detectable. Trypticase soy agar (TSA) or ... Cell wall sugars consist of galactose and glucose. The main fatty acids determined in this organism include 12- ... CSC uses glucose-1-phosphate and L-glutamic acid, and its colonies are more yellow in appearance as compared to its ...
Drug interaction
For example, it is known that hypoglycaemia (low blood glucose) in an organism produces a release of catecholamines, which ... One notable system involved in metabolic drug interactions is the enzyme system comprising the cytochrome P450 oxidases. ... like glucose or vancomycin), in which case they may or may not have specific and non-specific transporters distributed on the ... trigger compensation mechanisms thereby increasing blood glucose levels. The release of catecholamines also triggers a series ...
Agmatine
NADPH oxidase. Activation of the enzyme leading to H2O2 production. Agmatine sulfate injection can increase food intake with ... hypoglycemic effects are the result of simultaneous modulation of several molecular mechanisms involved in blood glucose ... Demady DR, Jianmongkol S, Vuletich JL, Bender AT, Osawa Y (January 2001). "Agmatine enhances the NADPH oxidase activity of ... An alternative pathway, mainly in peripheral tissues, is by diamine oxidase-catalyzed oxidation into agmatine-aldehyde, which ...
Dopexamine
It should not be used in people taking monoamine oxidase inhibitors, nor in people who have certain adrenal cancers, low ... Like other ß2-agonists, dopexamine lowers potassium levels and raises glucose levels, so there is a risk of exacerbating ...
Thermotoga hypogea
It is thermophilic, xylanolytic, glucose-fermenting, strictly anaerobic and rod-shaped. The type strain of T. hypogea is SEBR ... Yang, Xianqin; Ma, Kesen (2005). "Purification and characterization of an NADH oxidase from extremely thermophilic anaerobic ...
Ziprasidone
... is hepatically metabolized by aldehyde oxidase; minor metabolism occurs via cytochrome P450 3A4 (CYP3A4). ... August 22, 2007). "Effects of Olanzapine and Ziprasidone on Glucose Tolerance in Healthy Volunteers". Neuropsychopharmacology. ...
Amperometric Biosensor and Front-End Electronics for Remote Glucose Monitoring by Crosslinked PEDOT-Glucose Oxidase
... ... 2018). Amperometric Biosensor and Front-End Electronics for Remote Glucose Monitoring by Crosslinked PEDOT-Glucose Oxidase. ... film onto a polyethylene terephthalate/gold electrode followed by immobilization of cross-linked glucose oxidase by ... we show a novel low-cost and flexible biosensing platform for continuous glucose monitoring. The amperometric biosensing system ...
CAK1057 | Glucose Oxidase Microplate Assay Kit Clinisciences
GO2BS-Glucose oxidase
How can Glucose Oxidase be used? Glucose Oxidase (GO) is used in liquid and powder glucose laboratory reagents, urine test ... BBIs Glucose Oxidase is tried, tested and proven to perform in over 5 billion test strips every year. The high activity and ... colorimetric blood glucose strips and biosensors for blood glucose monitoring. What makes BBI a leading manufacturer of Glucose ... providing sensor and reagent manufacturers with a range of Glucose Oxidase products with proven performance. ...
GLUCOSE-OXIDASE SANDWICHED BETWEEN PHEMA LAYERS - A CONTINUOUS-FLOW REACTOR APPLICATION
Immobilization of glucose oxidase in conducting graft copolymers and determination of glucose amount in orange juices with ... IMMOBILIZATION OF GLUCOSE-OXIDASE - A COMPARISON OF ENTRAPMENT AND COVALENT BONDING. ARICA, MY; Hasırcı, Vasıf Nejat (1993-01- ... GLUCOSE-OXIDASE SANDWICHED BETWEEN PHEMA LAYERS - A CONTINUOUS-FLOW REACTOR APPLICATION. Date. ... Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl ...
Glucose oxidase - enzymes.bio
Glucose oxidase Puro Pó de Glicose Natural Oxidase CAS 9001-37-0 Enzima de Glicose Oxidase de Grau Alimentício. Rated 0 out of ... Glucose oxidase Fosfolipase Glucose Oxidase Enzimas Pó:10000u/g CAS 9001-37-0. Rated 0 out of 5 ... Glucose oxidase Glucose Oxidase Enzyme For Animal Feed Additives. Rated 0 out of 5 ... Glucose oxidase Glucose Oxidase Enzyme For Bakery Business. Rated 0 out of 5 ...
IMSEAR at SEARO: Glucose oxidase-induced lysis of erythrocytes.
Food Enzymes - Glucose Oxidase Manufacturer from Vadodara
Glucose Oxidase, Xylanase Enzyme, Transglutaminase offered by Infinita Biotech Private Limited, Vadodara, Gujarat. ... Pioneers in the industry, we offer glucose oxidase, xylanase enzyme and transglutaminase from India. ... It oxidizing glucose and producing gluconic acid and hydrogen peroxide.. To make Dough stronger and more elastic.. Strengthens ... It is used to stabilize liquid egg- white by converting glucose to D-gluconic acid.. Additional Information: ...
Glucose Oxidase| Leading Enzyme Manufacturer in India-Bioven Ingredients
EnzyChrom Glucose Oxidase Assay Kit | Technique alternative | 01014297288 - Enzychrome
Synthesis and Activity Results of Glucose Oxidase-Dextran Conjugates | AVESİS
Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases<...
Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases. / Moon, T. S.; Nielsen, D. R.; Prather ... Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases. AIChE J. 2012;58:2303-2308. ... title = "Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases", ... Sensitivity Analysis of a Proposed Model Mechanism for Newly Created Glucose-6-Oxidases. ...
Molecular effects of encapsulation of glucose oxidase dimer by graphene [email protected]
Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing<...
Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. In: Biosensors and ... Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. / Kang, Xinhuang; Wang, ... title = "Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing", ... Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing. ...
Isolation and screening of glucose oxidase producing Aspergillus oryzaestrain MF13 from marine source | Abstract
Glucose Oxidase(GOx) received numerous applicationsin the food industry and clinical fields. Marine fungi extensively ... Glucose Oxidase(GOx) received numerous applicationsin the food industry and clinical fields. Marine fungi extensively ... Isolation and screening of glucose oxidase producing Aspergillus oryzaestrain MF13 from marine source. Abstract. Author(s): ...
Baking Enzymes Manufacturers, Suppliers, Dealers & Prices
Agronomy | Free Full-Text | In-Vitro Inhibition of Pythium ultimum, Fusarium graminearum, and Rhizoctonia solani by a...
... enzymes were combined with glucose, potassium iodide, and ammonium thiocyanate to create an anti-fungal formulation, which ... Stabilized LPO and glucose oxidase (GOx) enzymes were combined with glucose, potassium iodide, and ammonium thiocyanate to ... glucose, lactoperoxidase (LPO, ≥150 U/mg), and glucose oxidase (GOx, 177 U/mg) were obtained from Sigma-Aldrich. ... Glucose Oxidase. Shoot Weight (g). SD 3. Root Weight (g). SD 3. Plant Height (cm). SD 3. ...
Glucose Oxidase Method Was Used to Remove Glucose from Egg Liquid - BESTZYME BIO-ENGINEERING CO., LTD.
The glucose oxidase method to remove the glucose, a type of industrial enzyme, in the egg white can be completely applied to ... After the egg white is desugared by the glucose oxidase method, the Maillard reaction can be effectively prevented from ... Enzymatic desugar is a method of using glucose oxidase to oxidize glucose in egg liquid to gluconic acid to desugar. It is ... 1. Desugaring effect of glucose oxidase method. Enzymatic desugar is a method of using glucose oxidase to oxidize glucose in ...
Tumor metabolism destruction via metformin-based glycolysis inhibition and glucose oxidase-mediated glucose deprivation for...
Glucose; Glucose Oxidase/farmacologia; Glucose Oxidase/uso terapêutico; Glicólise/efeitos dos fármacos; Humanos; Metformina/ ... Tumor metabolism destruction via metformin-based glycolysis inhibition and glucose oxidase-mediated glucose deprivation for ... Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Glucose Oxidase / ... Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Glucose Oxidase / ...
Glucose oxidase - Catalase Mixture (eukaryote)-Megazyme试剂盒 | Megazyme试剂盒|生物酶|糖标准品
Glucose oxidase: 9001-37-0, Catalase: 9001-05-2 Synonyms: Glucose oxidase: glucose oxidase; beta-D-glucose:oxygen 1- ... Glucose oxidase - Catalase Mixture (eukaryote)-Megazyme试剂盒. 发表于. 2022年1月11日. 由上海金畔生物科技有限公司 ... Glucose oxidase: 1.1.3.4, Catalase: 1.11.1.16 CAS Number: ... Glucose oxidase (12,000 U) plus Catalase (300,000 U) per vial ... High purity Glucose oxidase/Catalase Mixture (eukaryote) for use in research, biochemical enzyme assays and in vitro diagnostic ...
Kinetic evaluation and preformulation studies on Beta-D(+) glucose-glucose oxidase-catalase as an antioxidant system in...
The use of B-D(+) glucose-glucose oxidase (E.C. 1.1.3.4)- catalase (E. C. 1. 11. 1. 6) (BDG-GO-CAT) as a new antioxidant system ... Kinetic evaluation and preformulation studies on Beta-D(+) glucose-glucose oxidase-catalase as an antioxidant system in ... Post a Comment for Kinetic evaluation and preformulation studies on Beta-D(+) glucose-glucose oxidase-catalase as an ... Add tags for Kinetic evaluation and preformulation studies on Beta-D(+) glucose-glucose oxidase-catalase as an antioxidant ...
A layer-by-layer deposition of Concanavalin A and native glucose oxidase to form multilayer thin films for biosensor...
Anzai J, Kobayashi Y, Hoshi T, Saiki H. A layer-by-layer deposition of Concanavalin A and native glucose oxidase to form ... Anzai, J, Kobayashi, Y, Hoshi, T & Saiki, H 1999, A layer-by-layer deposition of Concanavalin A and native glucose oxidase to ... N2 - Glucose oxidase (GOx) can be assembled into multilayer thin films without any chemical modification, by an alternate ... AB - Glucose oxidase (GOx) can be assembled into multilayer thin films without any chemical modification, by an alternate ...
Effect of the modification of phenothiazine-labeled poly(ethylene oxide) on the solubility and enzymatic electrocatalytic...
keywords = "1-Butyl-3-methylimidazolium tetrafluoroborate, Electrocatalytic reaction, Glucose oxidase, Glucose oxidase hybrid, ... on the solubility and enzymatic electrocatalytic reaction of glucose oxidase in water/1-butyl-3-methylimidazolium ... the solubility and electrocatalytic properties are compared between native glucose oxidase (GOx) mixed with phenothiazine- ... on the solubility and enzymatic electrocatalytic reaction of glucose oxidase in water/1-butyl-3-methylimidazolium ...
Development of Crack-Free Alumina Sol-gel/Poly(vinyl Alcohol) Membranes for Glucose Oxidase Immobilization | Wong | ASEAN...
Journal of Chemical Sciences | Indian Academy of Sciences
The sensor uses benzoquinone as a mediator for electron transfer between immobilized glucose oxidase and a pyrolytic graphite ... A linear current response, proportional to glucose concentration in the range 1-50mM, is observed. The influence of oxygen,pH ... An amperometric enzyme sensor for the analysis of glucose is described. ...
Graphene in Biomedicine: Opportunities and Challenges
Enzimas microbianas na conversão da sacarose em frutose e ácido glicônico usando...
... and the oxidation of glucose to gluconic acid by glucose oxidase and catalase. Catalase was added in order to decompose the ... hydrogen peroxide an inhibitor of glucose oxidase formed as by-product of the oxidation. Two processes were employed. Fed-batch ... The best fed-batch performance was attained through the decreasing linear addition of sucrose (64g/L) and glucose (32g/L). ... in which the hydrolysis and oxidation reactions were carried out separately by adding invertase followed by glucose oxidase and ...
Peroxidase Type II, essentially salt-free, lyophilized powder, main 150-250units/mg solid using pyrogallol 9003-99-0
CatalaseDetermination of glucoseAssay KitEnzymaticNative glucose oxidaseEnzyme glucose oxidasePlasma glucoseImmobilizationGluconic acidBiosensorsMonoamine oxidase iTehran Lipid and Glucose StudyNADPHHydrogenEnzimaCholesterolImmobilized in conductingConcentrationGrapheneElectrodeSucroseConcentrationsGlicoseFructoseBloodDehydrogenaseMotileOxidationMembranesInhibitorsUptakeElectrochemistryPostprandialReactionMetabolismAmperometricOxygenIndiaDetect0.08InhibitionOptimum pHTemperature
Catalase9
- The currently used enzyme preparations from the enzyme factory contain not only glucose oxidase, but also catalase, which can decompose hydrogen peroxide in the egg liquid to form oxygen, but hydrogen peroxide needs to be continuously added to the egg liquid to avoid foaming and relieve the suppression of the reaction by the low concentration of the reactant. (bestzyme.com)
- High purity Glucose oxidase/Catalase Mixture (eukaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (jinpanbio.com)
- The use of B-D(+) glucose-glucose oxidase (E.C . 1.1.3.4)- catalase (E . C . 1 . (musc.edu)
- These studies indicate that B-D{+) glucoseglucose oxidase-catalase may be used as an effective antioxidant system in pharmaceutical solution formulations . (musc.edu)
- Mediante os resultados dos testes com os dois substratos, realizou-se teste de conversão direta da sacarose em frutose e ácido glicônico, utilizando-se invertase, glicose oxidase e catalase em regime descontínuo-alimentado, com alimentação linear decrescente (melhor resultado para ambos os substratos). (usp.br)
- This work dealt with the hydrolysis of sucrose by invertase into fructose and glucose, and the oxidation of glucose to gluconic acid by glucose oxidase and catalase. (usp.br)
- Catalase was added in order to decompose the hydrogen peroxide an inhibitor of glucose oxidase formed as by-product of the oxidation. (usp.br)
- Fed-batch in which the hydrolysis and oxidation reactions were carried out separately by adding invertase followed by glucose oxidase and catalase was conducted by adding the solution of substrate according to a constant, increasing linear, decreasing linear, increasing exponential or decreasing exponential mode. (usp.br)
- The isolate was motile, catalase positive, oxidase negative, and it fermented glucose and lactose. (cdc.gov)
Determination of glucose2
- For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism. (enzychrome.com)
- It is also used for the determination of glucose and peroxides in solution. (sigmaaldrich.com)
Assay Kit2
- The EnzyChrom Glucose Oxidase Assay Kit is designed for Research Use Only (RUO). (enzychrome.com)
- For use in removal of excess glucose in conjunction with the Sucrose/Glucose Assay Kit (K-SUCGL) and the Fructan HK Assay Kit (K-FRUCHK). (jinpanbio.com)
Enzymatic5
- Enzymatic desugar is a method of using glucose oxidase to oxidize glucose in egg liquid to gluconic acid to desugar. (bestzyme.com)
- The method can be used to determine the enzymatic activity of the system at glucose oxidase concentrations of 0.005 to 0.030 unit/ml , with an r2 of more than 0.995 for the linearity of the standard curve , and can be adapted for analysis in any pharmaceutical solution . (musc.edu)
- Imabayashi, SI , Ishii, K & Watanabe, M 2006, ' Effect of the modification of phenothiazine-labeled poly(ethylene oxide) on the solubility and enzymatic electrocatalytic reaction of glucose oxidase in water/1-butyl-3-methylimidazolium tetrafluoroborate mixtures ', Electrochemistry Communications , vol. 8, no. 1, pp. 45-50. (elsevier.com)
- The enzymatic glucose-oxidase method was employed in glucose estimation. (academicjournals.org)
- As starch cannot be fermented to ethanol by the traditional fermentation yeast Saccharomyces cerevisiae , the starch in the grains must first be degraded to fermentable sugars, glucose and maltose, by enzymatic degradation before fermentation. (biomedcentral.com)
Native glucose oxidase1
- In water/1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF 4 ]) mixtures, the solubility and electrocatalytic properties are compared between native glucose oxidase (GOx) mixed with phenothiazine-labeled poly(ethylene oxide) (PT-PEO) mediator and GOx-(PT-PEO) hybrid, in which PT-PEO is covalently attached to the surface lysine residues, under a substrate-saturated and diffusion-limited condition. (elsevier.com)
Enzyme glucose oxidase3
- In this study, matrix entrapment of the enzyme glucose oxidase was achieved through gamma-irradiation of monomers N-vinyl pyrrolidone, 2-hydroxyethyl methacrylate and their mixture. (metu.edu.tr)
- Oxidative stress in skin of B6C3F1 mice of both sexes was generated by intradermal injection of the hydrogen peroxide (H2O2) -producing enzyme, glucose oxidase with polyethylene glycol (GOD+PEG). (cdc.gov)
- This is because of the presence of the enzyme glucose oxidase in honey. (traya.health)
Plasma glucose6
- Fasting and postprandial plasma glucose screening can replace gestational diabetes mellitus screening in women who refuse to have the glucose load test. (who.int)
- The prevalence of GDM postprandial plasma glucose screening could replace is as high as 9.2%, according to a 2014 analysis ( 2 ). (who.int)
- Some hours later, the laboratory plasma glucose result (taken at the same time as the Medisense capillary sample) is available. (bestbets.org)
- plasma glucose: glucose oxidase method). (bestbets.org)
- Anthropometric parameters (height, weight and waist circumference), blood pressure, fasting plasma glucose, and serum lipids (HDL-cholesterol and triglycerides) were measured. (biomedcentral.com)
- Mean plasma glucose was found to be highest in the HbSSc state (84.80 ± 4.10 mg/dl), followed by HbSS (78.59 ± 4.20 mg/dl), HbAS (74.80 ± 6.20 mg/dl) and lowest in HbAA (70.10 ± 0.05 mg/dl). (academicjournals.org)
Immobilization4
- The working electrode is made of electropolymerized poly (3,4-ethylenedioxythiophene) film onto a polyethylene terephthalate/gold electrode followed by immobilization of cross-linked glucose oxidase by glutaraldehyde. (cam.ac.uk)
- Defects and functional groups of the GO are beneficial to immobilization of glucose oxidase (GOD), but sacrificing electron-transfer capability for highly-sensitive detection. (imr.ac.cn)
- In this work was developed a platform with a conductor material made of silica, titania and graphite carbon to be used as base for immobilization of gold nanoparticles and glucose oxidase enzyme. (ufrgs.br)
- Immobilization of glucose oxidase and urease in hydrogels of 2-hydroxyethyl methacrylae, and N-vinyl pyrrolidone (NVP) was achieved by irradiation (using UV kand gamma-rays). (metu.edu.tr)
Gluconic acid2
- It oxidizing glucose and producing gluconic acid and hydrogen peroxide. (infinitabiotech.in)
- It is used to stabilize liquid egg- white by converting glucose to D-gluconic acid. (infinitabiotech.in)
Biosensors3
- Glucose Oxidase (GO) is used in liquid and powder glucose laboratory reagents, urine test strips, colorimetric blood glucose strips and biosensors for blood glucose monitoring. (bbisolutions.com)
- This enzyme is widely used in making biosensors to detect the glucose levels in blood or serum. (bioveningredients.com)
- The biosensors based on glucose oxidase are of great importance for monitoring the glucose in blood, besides helping in diagnostic and treatment of diseases like diabetes. (ufrgs.br)
Monoamine oxidase i1
- Patients taking any monoamine oxidase inhibitor (MAOI) or within two weeks of taking an MAOI. (ebmconsult.com)
Tehran Lipid and Glucose Study1
- This study, part of the Tehran Lipid and Glucose Study, determined and compared the prevalence of overweight and its associated risk factors in 2 555, 1 329 and 1 158 Tehran children and adolescents in 2000, 2003 and 2006 respectively. (who.int)
NADPH2
- Febuxostat also suppressed NADPH oxidase expressions and enhanced SOD activity in HG-treated GEnCs. (asn-online.org)
- XO inhibition attenuates HG-induced oxidative stress through the activation of AMPK-PGC-1α-FoxO3a-NADPH oxidase signaling via purine salvage pathway. (asn-online.org)
Hydrogen4
- Glucose oxidase method for sugar removal should firstly use 10% organic acid to modulate protein solution (the yolk or whole egg solution does not need to add acid) to make pH reach about 7.0, then add 0.01% to 0.04% glucose oxidase, slowly stir and add at the same time 0.35% of 7% hydrogen peroxide. (bestzyme.com)
- Honey often contains significant quantities of glucose oxidase, an enzyme that promotes the production of hydrogen peroxide as honey ages. (alive.com)
- It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (bvsalud.org)
- Glucose oxidase can break down glucose sugars to produce hydrogen peroxide which can lighten the colour of your hair. (traya.health)
Enzima3
- Neste trabalho foi desenvolvida uma plataforma com um material condutor a base de sílica, titânia e carbono grafite para servir como matriz de imobilização de nanopartículas de ouro e enzima glicose oxidase. (ufrgs.br)
- Enzima de la clase de las oxidorreductasas, que cataliza la conversión de beta-D-glucosa y oxígeno a D-glucono-1,5-lactona y peróxido. (bvsalud.org)
- La enzima es producida por el Penicillium notatum y otros hongos y tiene actividad antibacteriana en presencia de glucosa y oxígeno. (bvsalud.org)
Cholesterol3
- Body mass index measurements were taken and blood pressure, fasting blood glucose, cholesterol and triglycerides measured. (who.int)
- Cholesterol oxidase has been immobilized in conducting copolymers of thiophene-3-yl acetic acid cholesteryl ester with pyrrole (CM/PPy) and polypyrrole (PPy) via electropolymerization. (metu.edu.tr)
- These are already recommended for reducing the blood pressure control and cholesterol levels of oxidase and burn serum potassium or a way to reduce blood pressure. (centrumlucina.cz)
Immobilized in conducting1
- Glucose oxidase was immobilized in conducting copolymers of three different types of poly(methyl methacrylate-co-thienyl methacrylate). (metu.edu.tr)
Concentration2
Graphene6
- We have performed molecular dynamics simulations to obtain an insight on many forms of contact between glucose oxidase dimer and the single-layer graphene nano-sheets. (iisc.ac.in)
- Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. (princeton.edu)
- This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. (princeton.edu)
- Graphene oxide (GO) has received considerable attention for glucose detection because of high surface area, abundant functional groups, and good biocompatibility. (imr.ac.cn)
- A graphene-laminated electrode with high glucose oxidase loading for highly-sensitive glucose detection[J]. JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY,2021,66:57-63. (imr.ac.cn)
- 2021).A graphene-laminated electrode with high glucose oxidase loading for highly-sensitive glucose detection. (imr.ac.cn)
Electrode2
- Glucose oxidase (GOx) can be assembled into multilayer thin films without any chemical modification, by an alternate deposition of Concanavalin A (Con A) and native GOx on the surface of electrode. (elsevier.com)
- The sensor uses benzoquinone as a mediator for electron transfer between immobilized glucose oxidase and a pyrolytic graphite electrode. (ias.ac.in)
Sucrose1
- Why do humans transport glucose but not sucrose like plants? (stackexchange.com)
Concentrations3
- BestBets: What is the normal range of blood glucose concentrations in healthy term newborns? (bestbets.org)
- Breast fed full term babies with low blood glucose concentrations produce ketones and other fuels as an adaptive mechanism. (bestbets.org)
- Sociodemographic and diagnosis variables (presence of dental calculus and periodontal status) were considered to measure salivary concentrations of glucose (by the glucose oxidase/peroxidase method, amylase (by the colorimetric test), urea (by the amount of indophenol), total protein (by the Bradford method) and albumin (by the nephelometric method). (bvsalud.org)
Glicose2
- Neste estudo, avaliou-se a hidrólise da sacarose pela invertase assim como a conversão da glicose em ácido glicônico, pela ação da glicose oxidase, ambas em processo descontínuo-alimentado. (usp.br)
- Os biossensores a base de glicose oxidase são de grande importância para monitorar a glicose no sangue, além de auxiliar no diagnóstico e tratamento de doenças como a diabetes. (ufrgs.br)
Fructose1
- Theoretical suggestion that the metabolism and plasma levels of some hexoses (glucose and fructose) in sickle cell anaemia could be altered was investigated. (academicjournals.org)
Blood15
- High blood glucose was defined as a fasting glucose of 126 mg/dL retinopathy, could worsen in Mexico, and the study of screening or more or, if fasting glucose was unavailable, as random glucose systems for diabetic retinopathy is important. (cdc.gov)
- A capillary heel prick blood test (Medisense) done on the postnatal ward has given a blood glucose reading of 2.6 mmol. (bestbets.org)
- Range of blood glucose was 1.4 to 5.3 mmol/l. (bestbets.org)
- The normal range of blood glucose is around 1.5-6 mmol/l in the first days of life, depending on the age of the baby, type of feed, assay method used, and possibly the mode of delivery. (bestbets.org)
- Up to 14% of healthy term babies may have blood glucose less than 2.6 mmol/l in the first three days of life. (bestbets.org)
- There is no reason to routinely measure blood glucose in appropriately grown term babies who are otherwise well. (bestbets.org)
- According to senior author Giuseppe Battaglia, there's a continuing increase in the gradient of glucose within blood vessels into the brain which had helped in guiding their pods through the barrier. (wallstreetpit.com)
- There was a general improvement in mean values of blood lipids, fasting blood glucose and HbA1c in all the groups, with NEP showing the greatest improvements, followed by NE, except for triglycerides and HDL where the control group had better improvement than the NE. (biomedcentral.com)
- The value for hypoglycemia is a blood glucose level of less than 50 mg/dL. (medscape.com)
- as the heart to be a clot or starting meditation of glucose, and why does sepsis lower blood pressure blood pressure control. (centrumlucina.cz)
- herbal supplements to lower blood sugar diabetes cure diet medical diabetes treatment and drugs Roche 2022 blood sugar balance Ozempic diabetes medications type 2 diabetes medications brands type 2 diabetes drugs side effects normal glucose levels for type 2 diabetes. (smartkitty.eu)
- In addition, infants were fitted with a device that monitored their blood glucose level continuously, charting their levels every 5 minutes. (smartkitty.eu)
- Gliclazide increases the release of insulin from the functioning of pancreatic beta cells to lower the blood glucose. (netmeds.com)
- GLEZNOR M 60 controls blood glucose levels, where gliclazide works by increasing the amount of insulin released by the pancreas in order to lower the blood glucose. (netmeds.com)
- Concomitant use of CETAL SINUS with tricyclic antidepressants, sympathomimetic agents (such as decongestants, appetite suppressants and amfetamine-like psychostimulants) or with monoamine oxidase inhibitors, which interfere with the catabolism of sympathomimetic amines, may occasionally cause a rise in blood pressure (see Contraindications ). (eipico.com.eg)
Dehydrogenase1
- Hemoglobinuria amongst adult Nigerians as a end result of glucose-6-phosphate dehydrogenase deficiency with drug sensitivity. (edu.hk)
Motile2
- Y enterocolitica is non-lactose-fermenting, glucose-fermenting, and oxidase-negative facultative anaerobe that is motile at 25°C and nonmotile at 37°C. Most, but not all, Y enterocolitica isolates reduce nitrates. (medscape.com)
- It is non-motile, non-sporeforming, and ferments glucose with the production of lactic acid. (quintessay.com)
Oxidation2
- That amount of enzyme causing the oxidation of one micromole of glucose per minute at 25C and pH 7.0. (bbisolutions.com)
- It is a flavoprotein, which catalyses the oxidation of glucose. (bioveningredients.com)
Membranes3
- Glucose oxidase was entrapped between poly(2-hydroxyethyl methacrylate) membranes and conditions were optimized for high enzyme activity and high levels of entrapment. (metu.edu.tr)
- Glucose oxidase was immobilized onto poly(2-hydroxyethyl methacrylate) membranes by covalent bonding through epichlorohydrin. (metu.edu.tr)
- Glucose oxidase was immobilized onto poly(2-hydroxyethyl methacrylate) (pHEMA) membranes by two methods: by covalent bonding through epichlorohydrin and by entrapment between pHEMA membranes. (metu.edu.tr)
Inhibitors3
- Xanthine oxidase (XO) contribute to reactive oxygen species (ROS) production, and XO inhibitors have been reported to the protection of kidney diseases. (asn-online.org)
- The efficiency of glipizide is likely to be altered by the influence of Monoamine Oxidase Inhibitors (MAOI's), diuretics and cimetidine. (petcarerx.com)
- Patients who are taking or have taken monoamine oxidase inhibitors within the preceding two weeks. (eipico.com.eg)
Uptake2
- of note, insulin is not required for the uptake of glucose by the intestinal cells. (medscape.com)
- Glucose is transported into the cells by an active, energy-requiring process that involves a specific transport protein and requires a concurrent uptake of sodium ions. (medscape.com)
Electrochemistry1
- Focusing on the interplay between interface chemistry, electrochemistry, and integrated electronics, we show a novel low-cost and flexible biosensing platform for continuous glucose monitoring. (cam.ac.uk)
Postprandial1
- 2-stage oral glucose tolerance test (OGTT) between 24 dL or the 2-hour postprandial or glucose challenge test and 28 weeks of pregnancy ( 6 ). (who.int)
Reaction2
- The reaction was first order with respect to glucose and to oxygen. (metu.edu.tr)
- After the egg white is desugared by the glucose oxidase method, the Maillard reaction can be effectively prevented from occurring during dry-heat treatment. (bestzyme.com)
Metabolism2
- Tumor metabolism destruction via metformin-based glycolysis inhibition and glucose oxidase-mediated glucose deprivation for enhanced cancer therapy. (bvsalud.org)
- Glucose measurements are used in the diagnosis and treatment of pancreatic islet cell carcinoma and of carbohydrate metabolism disorders, including diabetes mellitus, neonatal hypoglycemia, and idiopathic hypoglycemia. (cdc.gov)
Amperometric1
- An amperometric enzyme sensor for the analysis of glucose is described. (ias.ac.in)
Oxygen2
- An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. (bvsalud.org)
- The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. (bvsalud.org)
India1
- Pioneers in the industry, we offer glucose oxidase, xylanase enzyme and transglutaminase from India. (infinitabiotech.in)
Detect1
- ZnO nanorod-gated AlGaN/GaN HEMTs were used to detect glucose in EBC. (tamu.edu)
0.081
- The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 μA mM -1 cm -2 ) as compared with other nanostructured supports. (princeton.edu)
Inhibition2
- Herein, an intelligent nanomedicine based on glucose deprivation and glycolysis inhibition is creatively constructed for enhanced cancer synergistic treatment . (bvsalud.org)
- We investigated the cytoprotective mechanism associated with activated AMP-activated protein kinase (AMPK) by XO inhibition in high glucose (HG) condition. (asn-online.org)
Optimum pH2
- The optimum pH of glucose oxidase method for sugar removal is 3-8, generally, 6.7-7.2 is the best. (bestzyme.com)
- The optimum pH and temperature of free and immobilized glucose isomerase were found to be the same values as 7.5 and 60C, respectively. (metu.edu.tr)
Temperature1
- A ZnO nanorod array was selectively grown on the gate area using low temperature hydrothermal decomposition to immobilize glucose oxidase (GOx). (tamu.edu)