An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
Substances and materials manufactured for use in various technologies and industries and for domestic use.
Formal voluntary or governmental procedures and standards required of hospitals and health or other facilities to improve operating efficiency, and for the protection of the consumer.
An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.
Substances intended to be applied to the human body for cleansing, beautifying, promoting attractiveness, or altering the appearance without affecting the body's structure or functions. Included in this definition are skin creams, lotions, perfumes, lipsticks, fingernail polishes, eye and facial makeup preparations, permanent waves, hair colors, toothpastes, and deodorants, as well as any material intended for use as a component of a cosmetic product. (U.S. Food & Drug Administration Center for Food Safety & Applied Nutrition Office of Cosmetics Fact Sheet (web page) Feb 1995)
An application that must be submitted to a regulatory agency (the FDA in the United States) before a drug can be studied in humans. This application includes results of previous experiments; how, where, and by whom the new studies will be conducted; the chemical structure of the compound; how it is thought to work in the body; any toxic effects found in animal studies; and how the compound is manufactured. (From the "New Medicines in Development" Series produced by the Pharmaceutical Manufacturers Association and published irregularly.)
That segment of commercial enterprise devoted to the design, development, and manufacture of chemical products for use in the diagnosis and treatment of disease, disability, or other dysfunction, or to improve function.
Works about lists of drugs or collections of recipes, formulas, and prescriptions for the compounding of medicinal preparations. Formularies differ from PHARMACOPOEIAS in that they are less complete, lacking full descriptions of the drugs, their formulations, analytic composition, chemical properties, etc. In hospitals, formularies list all drugs commonly stocked in the hospital pharmacy.
Economic aspects of the fields of pharmacy and pharmacology as they apply to the development and study of medical economics in rational drug therapy and the impact of pharmaceuticals on the cost of medical care. Pharmaceutical economics also includes the economic considerations of the pharmaceutical care delivery system and in drug prescribing, particularly of cost-benefit values. (From J Res Pharm Econ 1989;1(1); PharmacoEcon 1992;1(1))
Organizations representing specialized fields which are accepted as authoritative; may be non-governmental, university or an independent research organization, e.g., National Academy of Sciences, Brookings Institution, etc.
Regulations to assure protection of property and equipment.
Laws and regulations concerned with industrial processing and marketing of foods.
Process that is gone through in order for a drug to receive approval by a government regulatory agency. This includes any required pre-clinical or clinical testing, review, submission, and evaluation of the applications and test results, and post-marketing surveillance of the drug.
Inorganic compounds that contain osmium as an integral part of the molecule.
A suspension of metallic gold particles.
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.
A suspension of radioactive gold particles emitting negative beta particles and gamma irradiation. It was formerly used for liver scans and irradiation treatment of some metastatic malignancies.
Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A flavoprotein enzyme that catalyzes the univalent reduction of OXYGEN using NADPH as an electron donor to create SUPEROXIDE ANION. The enzyme is dependent on a variety of CYTOCHROMES. Defects in the production of superoxide ions by enzymes such as NADPH oxidase result in GRANULOMATOUS DISEASE, CHRONIC.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
An imperfect fungus causing smut or black mold of several fruits, vegetables, etc.
A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.
Detailed account or statement or formal record of data resulting from empirical inquiry.
A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
A mitosporic fungal species used in the production of penicillin.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
An enzyme that catalyzes the hydrolysis of a single fatty acid ester bond in lysoglycerophosphatidates with the formation of glyceryl phosphatidates and a fatty acid. EC 3.1.1.5.
Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
Individual's rights to obtain and use information collected or generated by others.
A publication issued at stated, more or less regular, intervals.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Adherence of cells to surfaces or to other cells.
The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.
Glucose in blood.
A test to determine the ability of an individual to maintain HOMEOSTASIS of BLOOD GLUCOSE. It includes measuring blood glucose levels in a fasting state, and at prescribed intervals before and after oral glucose intake (75 or 100 g) or intravenous infusion (0.5 g/kg).
An iron-molybdenum flavoprotein containing FLAVIN-ADENINE DINUCLEOTIDE that oxidizes hypoxanthine, some other purines and pterins, and aldehydes. Deficiency of the enzyme, an autosomal recessive trait, causes xanthinuria.
An enzyme that catalyzes the oxidative deamination of naturally occurring monoamines. It is a flavin-containing enzyme that is localized in mitochondrial membranes, whether in nerve terminals, the liver, or other organs. Monoamine oxidase is important in regulating the metabolic degradation of catecholamines and serotonin in neural or target tissues. Hepatic monoamine oxidase has a crucial defensive role in inactivating circulating monoamines or those, such as tyramine, that originate in the gut and are absorbed into the portal circulation. (From Goodman and Gilman's, The Pharmacological Basis of Therapeutics, 8th ed, p415) EC 1.4.3.4.
A plant genus of the family ORCHIDACEAE that is the source of the familiar flavoring used in foods and medicines (FLAVORING AGENTS).
A plant genus of the family Paeoniaceae, order Dilleniales, subclass Dilleniidae, class Magnoliopsida. These perennial herbs are up to 2 m (6') tall. Leaves are alternate and are divided into three lobes, each lobe being further divided into three smaller lobes. The large flowers are symmetrical, bisexual, have 5 sepals, 5 petals (sometimes 10), and many stamens.
Time period from 1901 through 2000 of the common era.
Precise and detailed plans for the study of a medical or biomedical problem and/or plans for a regimen of therapy.

Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals. (1/420)

Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a reporter system based on the Aspergillus niger glucose oxidase. Strains harboring fusions of the ech42 or nag1 5' upstream noncoding sequences with the A. niger goxA gene displayed a glucose oxidase activity pattern that was consistent under various conditions with expression of the native ech42 and nag1 genes, as assayed by Northern analysis. The expression product of goxA in the mutants was completely secreted into the medium, detectable on Western blots, and quantifiable by enzyme-linked immunosorbent assay. nag1 gene expression was triggered during growth on fungal (Botrytis cinerea) cell walls and on the chitin degradation product N-acetylglucosamine. N-Acetylglucosamine, di-N-acetylchitobiose, or tri-N-acetylchitotriose also induced nag1 gene expression when added to mycelia pregrown on different carbon sources. ech42 expression was also observed during growth on fungal cell walls but, in contrast, was not triggered by addition of chitooligomers to pregrown mycelia. Significant ech42 expression was observed after prolonged carbon starvation, independent of the use of glucose or glycerol as a carbon source, suggesting that relief of carbon catabolite repression was not involved in induction during starvation. In addition, ech42 gene transcription was triggered by physiological stress, such as low temperature, high osmotic pressure, or the addition of ethanol. Four copies of a putative stress response element (CCCCT) were found in the ech42 promoter.  (+info)

Strictures in Crohn's disease are characterised by an accumulation of mast cells colocalised with laminin but not with fibronectin or vitronectin. (2/420)

BACKGROUND/AIMS: Intestinal fibrosis and stricture formation is an unresolved problem in Crohn's disease. The aim of this study was to investigate whether mast cells accumulate in these tissues and whether their localisation is associated with extracellular matrix components. METHODS: Mast cells were visualised by immunohistochemical staining of the mast cell specific proteases chymase and tryptase. Their localisation in relation to extracellular matrix components was shown by immunohistochemical double labelling. RESULTS: In strictures in Crohn's disease, a striking accumulation of mast cells was seen particularly in the hypertrophied and fibrotic muscularis propria, with a mean (SEM) mast cell number of 81.3 (14.9) v 1.5 (0.9)/mm(2) in normal bowel (p<0.0005). All mast cells in the muscularis propria were colocalised with patches of laminin. In contrast, in the submucosa, laminin was exclusively found in the basal lamina of blood vessels where many adherent mast cells were seen. No colocalisation of mast cells was found with fibronectin or vitronectin. CONCLUSIONS: The large accumulation of mast cells in the muscle layer of strictured bowel suggests a functional role for these cells in the hypertrophic and fibrotic response of the smooth muscle cells. The colocalisation with laminin indicates a mechanism of interaction between smooth muscle cells and mast cells that may be important in the role of mast cells in the process of fibrosis.  (+info)

Hydrogen peroxide production from reactive liposomes encapsulating enzymes. (3/420)

Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated in combination with horseradish peroxidase (HRP) or lactoperoxidase (LPO). The generation of hydrogen peroxide from the liposomes in response to externally added D-glucose substrate was monitored using a Rank electrode system polarised to +650 mV, relative to a standard silver-silver chloride electrode. The effects of encapsulated enzyme concentration, enzyme combinations (GO+HRP, GO+LPO), substrate concentration, electron donor and temperature on the production of hydrogen peroxide have been investigated. The electrode signal (peroxide production) was found to increase linearly with GO incorporation, was reduced on addition of HRP and an electron donor (o-dianisidine) and showed a maximum at the lipid chain-melting temperature from the anionic liposomes containing no cholesterol. To aid interpretation of the results, the permeability of the non-reactive substrate (methyl glucoside) across the bilayer membranes was measured. It was found that the encapsulation of the enzymes effected the permeability coefficients of methyl glucoside, increasing them in the case of anionic liposomes and decreasing them in the case of cationic liposomes. These observations are discussed in terms of enzyme bilayer interactions.  (+info)

Immunotargeting of glucose oxidase: intracellular production of H(2)O(2) and endothelial oxidative stress. (4/420)

Extracellular and intracellular reactive oxygen species attack different targets and may, therefore, result in different forms of oxidative stress. To specifically study an oxidative stress induced by a regulated intracellular flux of a defined reactive oxygen species in endothelium, we used immunotargeting of the H(2)O(2)-generating enzyme glucose oxidase (GOX) conjugated with an antibody to platelet-endothelial cell adhesion molecule (PECAM)-1, an endothelial surface antigen. Anti-PECAM-(125)I-GOX conjugates specifically bind to both endothelial and PECAM-transfected cells. Approximately 70% of cell-bound anti-PECAM-(125)I-GOX was internalized. The cell-bound conjugate was enzymatically active and generated H(2)O(2) from glucose. Use of the fluorescent dye dihydrorhodamine 123 revealed that 70% of H(2)O(2) was generated intracellularly, whereas 30% of H(2)O(2) was detected in the cell medium. Catalase added to the cells eliminated H(2)O(2) in the medium but had little effect on the intracellular generation of H(2)O(2) by anti-PECAM-GOX. Both H(2)O(2) added exogenously to the cell medium (extracellular H(2)O(2)) and that generated by anti-PECAM-GOX caused oxidative stress manifested by time- and dose-dependent irreversible plasma membrane damage. Inactivation of cellular catalase by aminotriazole treatment augmented damage caused by either extracellular H(2)O(2) or anti-PECAM-GOX. Catalase added to the medium protected either normal or aminotriazole-treated cells against extracellular H(2)O(2), yet failed to protect cells against injury induced by anti-PECAM-GOX. Therefore, treatment of PECAM-positive cells with anti-PECAM-GOX leads to conjugate internalization, predominantly intracellular H(2)O(2) generation and intracellular oxidative stress. These results indicate that anti-PECAM-GOX 1) provides cell-specific intracellular delivery of an active enzyme and 2) causes intracellular oxidative stress in PECAM-positive cells.  (+info)

Expression of amylase and glucose oxidase in the hypopharyngeal gland with an age-dependent role change of the worker honeybee (Apis mellifera L.). (5/420)

Worker honeybees change their behaviour from the role of nurse to that of forager with age. We have isolated cDNA clones for two honeybee (Apis mellifera L.) genes, encoding alpha-amylase and glucose oxidase homologues, that are expressed in the hypopharyngeal gland of forager bees. The predicted amino acid sequence of the putative Apis amylase showed 60.5% identity with Drosophila melanogaster alpha-amylase, whereas that of Apis glucose oxidase showed 23.8% identity with Aspergillus niger glucose oxidase. To determine whether the isolated cDNAs actually encode these enzymes, we purified amylase and glucose oxidase from homogenized forager-bee hypopharyngeal glands. We sequenced the N-terminal regions of the purified enzymes and found that they matched the corresponding cDNAs. mRNAs for both enzymes were detected by Northern blotting in the hypopharyngeal gland of the forager bee but not in the nurse-bee gland. These results clearly indicate that expression of the genes for these carbohydrate-metabolizing enzymes, which are needed to process nectar into honey, in the hypopharyngeal gland is associated with the age-dependent role change of the worker.  (+info)

Second harmonic generation of glucose oxidase at the air/water interface. (6/420)

We present a study of the adsorption of the glucose oxidase enzyme (GOx) at the air/water interface, using the nonlinear optical technique of surface second harmonic generation (SSHG). Resonant SSHG experiments were achieved by probing the pi-pi* transition of the flavin adenine dinucleotide (FAD) chromophores embedded in the GOx protein. Because of the subsequent resonance enhancement of the signal, the second harmonic (SH) wave arising from the GOx entities adsorbed at the interface was detectable for protein bulk aqueous concentrations as low as 70 nM. The protein adsorption was followed, and, at high GOx coverage, a change in the orientation of the FAD chromophore was observed, indicating either a rearrangement or a reorientation of the protein at the interface. Inasmuch as GOx is negatively charged at the biological pH of 7, its interactions with charged surfactants were also investigated. As expected, spreading positively charged surfactants onto a partial protein monolayer was found to increase the GOx surface concentration, whereas in the case of negatively charged surfactants, the GOx surface concentration decreased until the SH signal went back to the pure buffer solution response level. With the increasing GOx surface concentration, the rearrangement or reorientation of the protein was also observed.  (+info)

Imaging of biological macromolecules on mica in humid air by scanning electrochemical microscopy. (7/420)

Imaging of DNA, keyhole limpet hemocyanin, mouse monoclonal IgG, and glucose oxidase on a mica substrate has been accomplished by scanning electrochemical microscopy with a tungsten tip. The technique requires the use of a high relative humidity to form a thin film of water on the mica surface that allows electrochemical reactions to take place at the tip and produce a faradaic current (approximately 1 pA) that can be used to control tip position. The effect of relative humidity and surface pretreatment with buffer solutions on the ionic conductivity of a mica surface was investigated to find appropriate conditions for imaging. Resolution of the order of 1 nm was obtained.  (+info)

Conserved arginine-516 of Penicillium amagasakiense glucose oxidase is essential for the efficient binding of beta-D-glucose. (8/420)

The effects of mutation of key conserved active-site residues (Tyr-73, Phe-418, Trp-430, Arg-516, Asn-518, His-520 and His-563) of glucose oxidase from Penicillium amagasakiense on substrate binding were investigated. Kinetic studies on the oxidation of beta-D-glucose combined with molecular modelling showed the side chain of Arg-516, which forms two hydrogen bonds with the 3-OH group of beta-D-glucose, to be absolutely essential for the efficient binding of beta-D-glucose. The R516K variant, whose side chain forms only one hydrogen bond with the 3-OH group of beta-D-glucose, exhibits an 80-fold higher apparent K(m) (513 mM) but a V(max) only 70% lower (280 units/mg) than the wild type. The complete elimination of a hydrogen-bond interaction between residue 516 and the 3-OH group of beta-D-glucose through the substitution R516Q effected a 120-fold increase in the apparent K(m) for glucose (to 733 mM) and a decrease in the V(max) to 1/30 (33 units/mg). None of the other substitutions, with the exception of variant F418A, affected the apparent K(m) more than 6-fold. In contrast, the removal of aromatic or bulky residues at positions 73, 418 or 430 resulted in decreases in the maximum rates of glucose oxidation to less than 1/90. Variants of the potentially catalytically active His-520 and His-563 were completely, or almost completely, inactive. Thus, of the residues forming the active site of glucose oxidase, Arg-516 is the most critical amino acid for the efficient binding of beta-D-glucose by the enzyme, whereas aromatic residues at positions 73, 418 and 430 are important for the correct orientation and maximal velocity of glucose oxidation.  (+info)

Enzyme-based sensors have emerged as important analytical tools with application in diverse fields, and biosensors for the detection of glucose using the enzyme glucose oxidase have been widely investigated. In this work, the preparation of biosensors by electrochemical polymerization of (poly)thiophenes, namely 2,2′-bithiophene (2,2′-BT) and 4,4′-bis(2-methyl-3-butyn-2-ol)-2,2′-bithiophene (4,4′-bBT), followed by immobilization of glucose oxidase on the films, is described. N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide metho-|i|p|/i|-toluenesulfonate (CMC) was used as a condensing agent, and |i|p|/i|-benzoquinone (BQ) was used as a redox mediator in solution. The glucose oxidase electrodes with films of 2,2′-BT and 4,4′-bBT were then tested for their ability in detecting glucose from synthetic and real samples (pear, apricot, and peach fruit juices).
Research on lab-on-a-chip devices fabricated by traditional microfabrication techniques are gaining importance in the field of analytical chemistry. In particular there is an increasing demand for miniaturized biosensors based on enzymes owing to their applications in clinical analysis. With respect to Capillary Electrophoresis (CE) microchips, enzymes can impart a greater degree of selectivity. The technique of electrochemical polymerization was used to immobilize glucose oxidase on the CE microchip. Polypyrrole was used an immobilization matrix. The second chapter describes the experimental procedure for the immobilization of glucose oxidase on the CE microchip device. A two-step procedure was employed, which involved a thin layer of polypyrrole followed by a thick layer of polypyrrole/glucose oxidase film. The third chapter deals with the results obtained using a CE microchip ...
This study aimed to verify the performance of xylanase and its interaction with oxidants agents (glucose oxidase and ascorbic acid) on the quality of whole wheat bread. The experiment was based on a central composite rotational design and the Response Surface Methodology was used to analyze the results. None of the xylanase, glucose oxidase or ascorbic acid concentrations within the studied range led to a significant difference in the specific volume. The highest moisture content and the lowest firmness values were reported in the bread with lower and intermediate levels of xylanase and larger amounts of glucose oxidase and ascorbic acid. This effect was observed mainly at the end of the storage period. A minimum amount of xylanase (from 33 to 63 EDX kg-1 flour) showed to be essential for obtaining best results. Levels of ascorbic acid above 63mg kg-1 and glucose oxidase above 91 SRU kg-1 proved to be necessary to offer the beneficial effect of xylanase. © 2016, Universidade Federal de Santa ...
Find right answers right now! What are the advantages and disadvantages of the glucose oxidase method used for the measurement of glucose concentration in human samples? More questions about Science & Mathematics, what
Global Glucose Oxidase Market is estimated to be valued US$ XX.X million in 2019. The report on Glucose Oxidase Market provides qualitative as well as quantitative analysis in terms of market dynamics, competition scenarios, opportunity analysis, market growth, etc. for the forecast year up to 2029. The global glucose oxidase market is segmented on the basis of Type, Application, and geography ...
Microporous glass ceramic with skeleton of CaTi4(PO¬4)6 with average pore size of 12.7 nm has been synthesized and used as a carrier of glucose oxidase. The glass ceramic was prepared by controlled heat treatment of glass samples, which causes the phase separation in their structure and creates CaTi4(PO¬4)6 and β-Ca3(PO4)2 phases. The β-Ca3(PO4)2 phase was dissolved by soaking the glass ceramics in HCl and CaTi4(PO¬4)6 built the skeleton of microporous glass ceramic. Analysis of the ability of the carrier for immobilization of glucose oxidase (GOx) was undertaken. Average amount of immobilized enzyme and percentage of enzyme activity on the carrier were 27 gr GOx/gr carrier and 60.15%, respectively. Effect of pH and temperature variations on the enzyme activity has been studied and results demonstrated that maximum activity for both free and immobilized enzyme was at T=40˚C and pH=7.0. Due to the same value of maximum activity, no serious conformational change of enzyme had taken place through
Effects of Wheat Flour Dough’s Viscoelastic Level by Adding Glucose Oxidase on its Dynamic Shear Properties whatever the Strain Modes
Abstract: Nano-carbon aerogels (CA) were prepared by the reaction between resorcinol and formaldehyde in alkaline conditions. The morphology and microstructure of the CA was investigated by scanning electron microscopy and transmission electron microscopy. The specific area and the pore size distribution were calculated from the N2 adsorption-desorption isotherm. Glucose oxidase (GOD)/CA/ glassy carbon (GC) electrodes were prepared by immobilizing GOD on the surface of nano-carbon aerogels. The electrocatalytic properties of GOD/CA/GC electrodes were characterized by cyclic voltammetry in phosphate buffer medium (0.1 mol·L-1). The results showed that GOD can be immobilized on the nano-carbon aerogels, and that the biological activation of GOD was retained. The GOD/CA/GC electrode exhibits good direct electrochemical and electro-catalytic performance without electron mediators.. Key words: Carbon aerogel, Glucose oxidase, Direct electrochemistry ...
TY - JOUR. T1 - Kinetic limitations of a bioelectrochemical electrode using carbon nanotube-attached glucose oxidase for biofuel cells. AU - Zhao, Xueyan. AU - Jia, Hongfei. AU - Kim, Jungbae. AU - Wang, Ping. PY - 2009/12/15. Y1 - 2009/12/15. N2 - Carbon nanotubes (CNTs) have been used for various bioelectrochemical applications, presumably for substantial improvement in performance. However, often only moderate results observed, with many governing factors have been considered and suggested yet without much systematic evaluation and verification. In this study, CNT-supported glucose oxidase (CNT-GOx) was examined in the presence of 1,4-benzoquinone (BQ). The intrinsic Michaelis parameters of the reaction catalyzed by CNT-GOx were found very close to those of native GOx. However, the Nafion entrapment of CNT-GOx for an electrode resulted in a much lower activity due to the limited availability of the embedded enzyme. Interestingly, kinetic studies revealed that the biofuel cell employing such ...
This article presents a study of glucose oxidase (GOx) immobilization by employing tannic acid (TA) modified-CoFe2O4 (CFO) magnetic nanoparticles which demonstrates novel aspect for enzyme immobilization. By using the strong protein and tannic acid binding, GOx immobilization was carried out via physical adsorption in a simpler way compared with the other immobilization methods which require various chemicals and complicated procedures which is difficult, expensive, time-consuming, and destructive to the enzyme structure. CFO was synthesized by hydrothermal synthesis and modified with TA to immobilize GOx. The immobilized GOx demonstrated maximum catalytic activity at pH 6.5 and 45°C. The samples were characterized by vibrating sample magnetometer (VSM), thermogravimetric analysis (TGA), zeta potential, and fourier transform infrared spectroscopy (FTIR), all of which confirm the surface modification of CFO and GOx immobilization. Also, field emission scanning electron microscope (FESEM) and ...
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It is known that regulation of plant tolerance to adverse environmental factors is connected with short term increase of the concentration of endogenous reactive oxygen species (ROS), which are signalling molecules for the induction of protective mechanisms. Introduction and expression of heterologous gox gene, which encodes glucose oxidase enzyme in plant genome, induce constantly higher content of hydrogen peroxide in plant tissues. It is not known how the introduction of native or modified gox gene affects the plant resistance to high-temperature stress, one of the most commonly used model for the study of stress response and thermal tolerance. In this study, we investigated biological effects of transformation and evaluated the resistance to temperature stress of potato plants with... altered levels of glucose oxidase expression. Transformation of potato plants by gox gene led to the more early coming out from tuber dormancy of transformed plants and slower growth rate. Transformants ...
Based on electric conductivity and wide potential window of ionic liquid (IL) and electric property of single-wall car- bon nanotubes (SWCNTs), composite material of IL-SWCNTs was prepared, glucose sensor was built with this mate-rial for immobilizing glucose oxidase (GOx). It showed good response, sensitivity and stability for long time for glu-cose detection. Linear range for the detection of glucose was from 0.5 × 10-6 M to 12 × 10-6 M while detection limit was 6.26 × 10-8 M (S/N = 3).
Title:Analyzing the Amperometric Response of a Glucose Oxidase Sensor Applying Mathematical Models. VOLUME: 12 ISSUE: 6. Author(s):Felix F. González Navarro, Margarita Stoytcheva, Lluís A. Belanche Munoz, Brenda L. Flores Rios, Jorge E. Ibarra Esquer, Livier Rentería Gutiérrez and Gabriel A. López Morteo. Affiliation:Engineering Institute, Autonomous University of Baja California; Mexicali, Mexico.. Keywords:Glucose oxidase amperometric sensor, machine learning, regression models, biosensor modeling.. Abstract:Background: The biosensors are analytical devices combining a bioreceptor and a physicochemical transducer to translate the signal resulting from the interaction of the analyte with the biological element into an electrical reading. The technological attractiveness of the biosensors resides in their high sensitivity, continuous measuring capabilities, low cost, and precision. An important and promising area of biosensors application is the associated with blood glucose levels ...
A thin-walled graphitic nanocages material with well-developed graphitic structure, large specific surface area and pronounced mesoporosity was synthesized and used to construct a sensing interface for an amperometric glucose biosensor, showing a high and reproducible sensitivity of 13.3 μA mM−1 cm−2, linear dynamic range of 0.02−6.2 mM, and fast response time of 5 s. It was successfully used to accurately detect glucose in human serum with effective discrimination to common interference species such as dopamine, ascorbic acid, acetaminophen, and uric acid ...
Antibacterial ? How long ? and what if diluted ?. Honey is claimed to be anti microbial by osmosis (high concentration on particles such as glucose and saccharose) and by producing Hydrogen peroxide (H2O2) when in contact with water. The latter is because some honeys do contain the enzyme glucose oxidase that converts glucose in glucon acid and H2O2. Hydrogen peroxide is a potent anti microbial agent that is effective in small quantities against most microorganisms. If honey is heated or irradiated the glucose oxidase will be destroyed.. To check if the glucose oxidase is active enough even when the Melladerm® Plus is diluted heavily we ordered a Zone of inhibition test in a certified independent laboratory.. Zone of Inhibition test. A zone of inhibition test was performed showing that Melladerm® Plus could be diluted 30 times before losing its antibacterial activity against Staphylococcus aureus. To execute the actual assay, we choose a LMG-reference culture, i.e. Staphylococcus aureus subsp. ...
A wearable-type glucose sensor for continuously or intermittently determining the glucose content comprises a short hollow fiber (2) to be positioned in the subcutaneous tissue. This hollow fiber is connected via tubes (3,4) with component parts (5 . . . 12) located outside the body, such as a measuring unit (11). When a perfusion fluid containing the enzyme glucose oxidase is passed through the hollow fiber, a subcutaneous dialysis will take place in which some glucose dissolves in the perfusion fluid through the wall of the hollow fiber. This glucose is completely oxidized by the oxygen dissolved in the perfusion fluid in the presence of the glucose oxidase. By means of the measuring unit the resultant amount of H2 O2 or, preferably, the employed amount of O2 is determined, both of which are a measure of the subcutaneous glucose concentration.
An interesting observation of this study is that insulin-induced Rac activation and actin remodeling appear to be more sensitive to disruption by glucose oxidase than is Akt phosphorylation: Rac activation was reduced by ,60% at 12.5 mU/ml glucose oxidase (Fig. 4), whereas Akt phosphorylation at either Thr308 or Ser 473 had only begun to be affected by twice those concentrations (Fig. 6). A slight difference was also observed with C2-ceramide, which at 12.5 μmol/l effectively reduced Rac activation, yet Akt Ser473 phosphorylation remained intact. However, Thr308 phosphorylation was as sensitive to C2-ceramide as was Rac activation.. Insulin-induced GLUT4 translocation was also somewhat more susceptible to inhibition by glucose oxidase and C2-ceramide than was Akt phosphorylation. Defects in GLUT4 translocation were apparent at 12.5 mU/ml glucose oxidase or 12.5 μmol/l C2-ceramide, and complete inhibition was achieved with 50 mU/ml glucose oxidase or 50 μmol/l C2-ceramide, concentrations that ...
An interesting observation of this study is that insulin-induced Rac activation and actin remodeling appear to be more sensitive to disruption by glucose oxidase than is Akt phosphorylation: Rac activation was reduced by ,60% at 12.5 mU/ml glucose oxidase (Fig. 4), whereas Akt phosphorylation at either Thr308 or Ser 473 had only begun to be affected by twice those concentrations (Fig. 6). A slight difference was also observed with C2-ceramide, which at 12.5 μmol/l effectively reduced Rac activation, yet Akt Ser473 phosphorylation remained intact. However, Thr308 phosphorylation was as sensitive to C2-ceramide as was Rac activation.. Insulin-induced GLUT4 translocation was also somewhat more susceptible to inhibition by glucose oxidase and C2-ceramide than was Akt phosphorylation. Defects in GLUT4 translocation were apparent at 12.5 mU/ml glucose oxidase or 12.5 μmol/l C2-ceramide, and complete inhibition was achieved with 50 mU/ml glucose oxidase or 50 μmol/l C2-ceramide, concentrations that ...
In this paper we show that hyperbranched polymers can be used as a host matrix for electrostatic entrapment of enzymes. Specifically, amine-functionalized glucose oxidase (GOx+) and horseradish peroxidase, as well as poly(amidoamine) dendrimer-modified horseradish peroxidase, reversibly sorb into polyanionic, hyperbranched poly(sodium acrylate) (PAA-) films that are on the order of a few hundred angstroms thick. The quantity of GOx+ entrapped within the PAA- films depends on the nature of film preparation but is typically on the order of 0.06 unit/cm2. The extent to which entrapped GOx+ retains its activity depends on the film history, but for PAA-/GOx+ composites not exposed to glucose and stored at 4 degrees C, the original activity is retained for up to 68 days and perhaps longer.
Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.
The sugar oxidising enzymes glucose oxidase, glucose dehydrogenases (GDH) and cellobiose dehydrogenases (CDH) were co-immobilised, in the presence of multiwalled carbon nanotubes, with osmium redox polymers. Under pseudo-physiological conditions of 5 mM glucose, 150 mM NaCl, 37?degrees C, glucose oxidation current densities above 800 mu A?cm-2 are obtained from films containing an [Os(4,4xxx-dimethyl-2,2xxx-bipyridine)2(poly-vinylimidazole)10Cl]+ redox polymer, redox potential 0.1 V vs. Ag/AgCl, and either glucose oxidase or FAD-dependant GDH. Current produced by, and stability of, glucose-oxidising half-cells is compared in 100 mM glucose, with films containing CDHs proving most stable. Such results show promise for development of glucose-oxidising enzymatic fuel cells ...
In this work, a novel chemiluminescence (CL) flow biosensor for glucose was proposed. Glucose oxidase (GOD), horseradish peroxidase (HRP) and gold nanoparticles were immobilized with sol-gel method on the inside surface of the CL flow cell. The CL de
A gene of glucose oxidase (GOD) from Aspergillus niger Z-25 was cloned and sequenced. The entire open reading frame (ORF) consisted of 1,818 bp and encoded a putative peptide of 605 amino acids. The gene was fused to the pPICZαA plasmid and overexpressed in Pichia pastoris SMD1168. The recombinant GOD (rGOD) was secreted into the culture using MF-α factor signal peptide under the control of the AOX1 promoter. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that rGOD exhibited a single band at around 94 kDa. The maximal GOD activity of approximately 40 U/mL was achieved in shake flask by induction under optimal conditions after 7 days. rGOD was purified by ammonium sulfate precipitate leading to a final specific activity of 153.46 U/mg. The optimum temperature and pH of the purified enzyme were 40 °C and 6.0, respectively. Over 88% of maximum activity was maintained below 40 °C. And the recombinant enzyme displayed a favorable stability in the pH range from 4.0 to 8.0. The ...
glucose oxidase from aspergillus niger/ACM9001370 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
4665-7604 recognises glucose oxidase from Aspergillus niger.This antibody has not been cross-adsorbed and may react with homologous proteins
A glucose oxidase (GOd) bioelectrode exhibiting high performance, direct electron transfer (DET) has been prepared. Unprecedented redox peak current densities of 1 mA cm(-2) were observed alongside a clear electrochemical response to glucose. This system shows potential as a low cost, high performance enzymatic bioelectrode.. ...
Page contains details about three-dimensional nitrogen-doped graphene/chitosan/glucose oxidase . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
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View Notes - 0825 from BME 511 at USC. Desired blood glucose level Insulin Insulin-release Glucose Glucose sensor Measured blood glucose Questions to be answered : 1. Dynamics of the system -
1CF3: 1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes.
In vivo brain glucose measurements: differential normal pulse voltammetry with enzyme-modified carbon fiber microelectrodes.: The enzyme glucose oxidase was imm
Adiy bin Hatim berkata: Ketika turun ayat kelenjar ini adalah menghasilkan hormon insulin. Diabetic Aikido Acidosis List Grocery Store but our liary can be accessed from certain countries only. This classic Jamaican dish is low in fat yet high in fiber protein and calcium. Home , Managing Your Diabetes , Kitbags and carry cases. No More Diabetes Testing Using Blood? The diabetes test strips used in currently available glucose monitoring systems contain the enzyme glucose oxidase. Diabetes Research Projects Article Its a safe forum where you can create or participate in support groups and discussions about health topics that interest you.. Young-onset type 2 diabetes mellitus (T2DM) is associated with greater mortality increased complications and unfavorable cardiovascular risk versus type 1 diabetes The ideal for your sugar before you eat in the morning is Dr. Diabetes Symptoms Tingling Feet It seems keeping you sick and marginally pain-free is all any ones really to do for you doing so means ...
i had such an overwhelming response asking for this reference im just going to post it here for all. Andrew SM, Jasani B (1987) Histochem J 19:426-30 best of luck to all who try it....i think its a great protocol for use on frozens. it does take a bit of time....you incubate for 1hr. @ 37C. carrie kyle-byrne [email protected] ...
Suggested Use: After cleansing and toning your face in the evening, apply a thin layer of Hyaluronic Acid PM Creme to face and neck. Use daily for best results. Ingredients: De-ionized Water, Stearic Acid (vegetable source), Avocado Oil, Glyceryl Stearate, Cetyl Alcohol, Vegetable Glycerin, Cetearyl Alcohol/Cetearyl Glucoside, Palmitic Acid Ester, Vegetable Squalane, Soy Lecithin, Natural Aroma, Glucose/Glucose Oxidase/Lactoperoxidase (natural preservative), Cetyl Palmitate, Shea Butter (tree nut), Manago Butter, Jojoba Oil, Sodium Hyaluronate (Hyaluronic Acid), Potassium Sorbate, MSM, L-Carnosine, Xanthan Gum, Tetrahexyldecyl Ascorbate (Vitamin C), d-alpha Tocopherol (Vitamin E), Soluble Collagen, Grapefruit Seed Extract and Green Tea Extract. NOW does not test on animals. Please Recycle ...
An immobilized enzyme electrode effective in measurement of the substrate concentration of the enzyme and in conversion from enzyme reaction energies into electric energies. The immobilized enzyme, of an oxidase system, such as glucose oxidase, amino acid oxidase, xanthine oxidase or the like and a metal oxide capable of constituting a redox system which is reduced through coupling with these enzyme reactions and is electrochemically oxidized (anodic oxidation) are combined with each other. The use of the enzyme electrode allows the determination quantity of the enzyme substrate as extremely low as approximately 10-5 to 10-6 mole/l in concentration.
OBJECTIVE: To determine the day-to-day intraindividual variability of fasting plasma glucose (FPG) in newly diagnosed Caucasian type 2 diabetic subjects. RESEARCH DESIGN AND METHODS: A total of 193 newly diagnosed, previously untreated, Caucasian type 2 diabetic subjects (135 men, 58 women) had FPG measured on two consecutive days (FPG1, FPG2). Ethical approval and subjects full informed consent were obtained. Subjects fasted for 12 h before each study day and rested for at least 30 min before blood was taken. Plasma glucose was analyzed by a glucose oxidase method with intra- and interassay coefficients of variation (CVs) , 2%. Variability of FPG was assessed by comparison of percentage differences (PDs): PD = 100 (FPG2 - FPG1)/FPG1, with averaged FPG (FPGaver = [FPG1 + FPG2]/2). Biological and analytical variability were determined by use of SD2total = SD2biological + SD2analytical, where SD2analytical approximately equal to 2 x (CVglucose measurement)2. Given normally distributed data with ...
There is a peroxidase quench that will block ALL forms of peroxidase, totally. This is the glucose oxidase method. Hsu H-M, et al Am J Pathology 188:209-217,1984 Andrew SM and Jasani G Histchem J 19:426-430, 1987 Vector provided me with the exact protocol ,Date: Mon, 10 Jul 2000 14:43:03 -0400 ,From: Jeff Crews ,[email protected], ,Subject: Re: IH on Liver Tissue ,To: [email protected], [email protected] , , With extremely bloody tissue like spleen or liver, it is sometimes , impossible to quench all of the peroxidase present in the blood. Try , an alkaline-phoshatase detection system. , , Jeffrey Crews, HTL (ASCP) , Organogenesis, Inc. , , ,______________________________ Reply Separator _________________________________ ,Subject: IH on Liver Tissue ,Author: ,[email protected], at internet ,Date: 07/10/2000 11:03 AM , , ,How can you successfully perform immunohistochemistry on slides of liver ,tissue using horse-radish peroxidase and DAB? How do you quench the ,endogenous peroxidase ...
ice for 5 min to avoid coagulation and then centrifuged at 12 0006 g for 2 min at 4uC to pellet the hemocytes. The supernatant was then collected. Glucose concentration (mg dL21) in the hemolymph was assessed using the glucose oxidase method of a commercial kit (Hospitex Diagnostics).Criteria for Choosing Experimental CrayfishOnly hard-shelled, intact, and sexually mature males were used for the experiment. A total of 80 individuals (cephalothorax length: 47.560.6 mm) were thus selected: 20 for the extraction of cHH and 60 for behavioural observations. Since purchase Salmon calcitonin dominance increases with body size in crayfish [3], the experimental pairs of fighting males were size matched (maximum difference in cephalothorax length: 62 mm) to eliminate any factor that could induce an obvious bias to our experiments. Before the beginning of the experiment, crayfish were kept in isolation in opaque plastic aquaria (25615625 cm) for at least two weeks, which is a sufficient time to reset any ...
Plasma-glucose levels have been determined by a glucose oxidase method in newborn infants of normal mothers (INM) and of diabetic mothers (IDM).
Sensitive Enzymatic Assay for Glucose Determination in Natural Waters 1: A new enzymatic method for glucose determination is described. It allows measurement of
A non-mediated enzyme electrode comprises a base substrate (2) on which is provided an electrically conductive base layer (8) comprising finely divided platinum group metal or oxide bonded together by a resin; a top layer on the base layer (8), the top layer comprising a buffer. A catalytically active quantity of an oxidoreductase enzyme is provided in at least one of the base layer and the top layer. The invention also provides a biosensor (20) which includes an enzyme electrode, and methods of manufacturing the enzyme electrode and biosensor.
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Glucose Oxidase (GO) enzyme (~200-300 u/mg) Pure Protein GO11-N-250 Glucose Oxidase (GO) enzyme (~200-300 u/mg) Pure Protein GO11-N-250
A team led by Tom Soh, a Project Leader for The Institute for Collaborative Biotechnologies has fashioned a small electronic device that can continuously track the level of medicines in an animals bloodstream. If it works in people, the device could revolutionize how medicines such as anticancer compounds and antibiotics are monitored and administered for life-threatening conditions.. Doctors always aim to give their patients the right dose of medications. But thats often not easy. Drug levels typically spike above the desired level after a medicine is administered and then fall below it as time goes on. Its been even harder to track drugs with a continuous electronic readout. A device does exist for measuring glucose in the bloodstream of diabetics. It works by tethering an enzyme called glucose oxidase next to an electrode. When a drop of blood is added to the device, the glucose oxidase splits glucose molecules, swiping electrons in the process. Those electrons then hop to the electrode, ...
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2014-12-4 · Historical assay formats for point-of-care testing are dipsticks and lateral flow devices. In the 1950s, the earliest paper-based strip test emerged for the quantification of the concentration of glucose in urine, and this product was marketed in the 1960s [].The principle of operation of the assay was based on glucose oxidase, and developed colours were compared to a reference chart …. Get Price ...
The factor was glucose oxidase. In the presence of glucose, the glucose oxidase produced hydrogen peroxide, which killed off ... "Detlev Müller discovers glucose oxidase". Tacomed.com. Archived from the original on 18 April 2018. Retrieved 13 June 2017. CS1 ... He is best known for discovering the enzyme glucose oxidase. In 1928, he was experimenting with the common fungus, Aspergillus ... He eventually found that these bacteria could only thrive adjacent to Aspergillus niger if glucose was not present. He ...
In a study by Oxenbøll et al., C. oxysporum catalyzed glucose oxidation by producing glucose oxidase, which it is mentioned in ... that the glucose oxidase helped protect against bacterial infection on the surface of fungi. The organism is also proven to be ... Oxenbøll, K; Si, J. Q; Aagaard, J (3 May 1995). "Alkaline glucose oxidase obtained from Cladosporium oxysporum". US Grant. ... It breaks down cellulose from dead herbaceous or plants, which is then further turned into glucose to Be used by the fungi ...
"Thermal stability of Penicillium adametzii glucose oxidase". Prikladnaia biokhimiia i mikrobiologiia. 37 (6): 678-86. PMID ...
"Glucose oxidase-magnetite nanoparticle bioconjugate for glucose sensing". Analytical and Bioanalytical Chemistry. 380 (4): 606- ...
... glucose oxidase (P13006), used in the design of glucose biosensors, due to its high affinity for β-D-glucose. Aspergillus niger ... "Nano-Encapsulation of Glucose Oxidase Dimer by Graphene". RSC Advances. 5 (18): 13570-78. doi:10.1039/C4RA16852F. S2CID ... Staiano, M.; Bazzicalupo, P.; Rossi, M.; d'Auria, S. (2005). "Glucose biosensors as models for the development of advanced ...
Commercially available glucose monitors rely on amperometric sensing of glucose by means of glucose oxidase, which oxidises ... which uses the enzyme glucose oxidase to break blood glucose down. In doing so it first oxidizes glucose and uses two electrons ... "Nano-Encapsulation of Glucose Oxidase Dimer by Graphene". RSC Advances. 5 (18): 13570-78. doi:10.1039/C4RA16852F. Daaboul, G.G ... Measured glucose data will be transmitted wirelessly out of the body within the MICS 402-405 MHz band as approved for medical ...
"Reengineered glucose oxidase for amperometric glucose determination in diabetes analytics". Biosens. Bioelectron. 50: 84-90. ... glucose oxidase for analytical application, phytase with increased thermostability and monooxygenase with improved catalytic ...
For example , the immobilization of glucose oxidase on calcium alginate gel beads can be used in a bioreactor. The resulting ... Examples include entrapment of enzymes such as glucose oxidase in gel column for use as a bioreactor. Important characteristic ... "Production of gluconic acid with immobilized glucose oxidase in airlift reactors". Chemical Engineering Science. 52 (21-22): ... Kinetic studies of Immobilized glucose oxidase". Biotechnology and Bioengineering. 16 (2): 159-168. doi:10.1002/bit.260160202. ...
... bees use the enzyme glucose oxidase, stored in their hypopharyngeal glands. Glucose oxidase works to ... Burgett, D. Michael (January 9, 1974). "Glucose Oxidase: A Food Protective Mechanism in Social Hymenoptera" (PDF). Annals of ...
Glucose oxidase-like gold nanoparticles combined with cyclodextran were used for chiral catalysis. An artificial binuclear ... Glucose oxidase and iron oxide nanozymes were encapsulated within multi-compartmental hydrogels for incompatible tandem ... Pd12 coordination cage as a photoregulated oxidase-like nanozyme was developed. An NADPH oxidase-like nanozyme was developed. A ... with glucose oxidase (GOx) in sub-micron proximity, providing a fast and efficient enzyme cascade reported as monitoring ...
Biosensors/diagnostics, e.g. glucose oxidase and cholesterol oxidase biosensors. Delivery of proteins as therapeutic agents or ...
The three primary enzymes in Biotène are[were? no longer, per the above] Glucose Oxidase, Lactoperoxidase, and Lysozyme. ... and glucose oxidase. People with xerostomia (dry mouth) may use Biotène to reduce the rate of recurrence of dental plaque. ... and prior to the GlaxoSmithKline acquisition it also contained enzymes including glucose oxidase, lactoferrin, lactoperoxidase ...
Now, however, the membrane is impregnated with immobilized glucose oxidase (GOx). The GOx will consume some of the oxygen as it ... leaving as the only variable the oxygen and glucose concentrations on the analyte-side of the glucose membrane, which is the ... The Clark oxygen electrode laid the basis for the first glucose biosensor (in fact the first biosensor of any type), invented ... The rate of reaction current is limited by the diffusion of both glucose and oxygen. This diffusion can be well characterized ...
GNP-glucose oxidase monolayer electrode was constructed use these two methods. The Au NP allowed more freedom in the enzyme's ... These ribbons are very flexible and can bend with angles larger than 90°. When glucose is replaced by cyclodextrin (a glucose ... In another method using ultrasound, the reaction of an aqueous solution of HAuCl4 with glucose, the reducing agents are ... Large AuNPs conjugated with biotin, cysteine, citrate, and glucose were not toxic in human leukemia cells (K562) for ...
Petruccioli, M.; Federici, F. (1993). "Glucose oxidase production by Penicillium variabile P16: Effect of medium composition". ... "Expression of Penicillium variabile P16 glucose oxidase gene in Pichia pastoris and characterization of the recombinant enzyme ...
"Glucose-sensitive membranes containing glucose oxidase: Activity, swelling, and permeability studies". Journal of Biomedical ... The major focus has been the development of a non-invasive continuous detection of glucose. The first clinical study on ... Since, Kost also proposed a novel approach for a glucose flux continuous biosensor and noninvasive detection of amniotic fluid ... In addition to his studies on responsive controlled release systems: pH sensitive, glucose sensitive and calcium sensitive drug ...
These glucose sensitive hydrogels are modified with the enzyme glucose oxidase. In the presence of glucose, the glucose oxidase ... An example of this would be hydrogels that release insulin in the presence of high glucose levels in the bloodstream. ...
Studies of Enzyme-Mediator Kinetics for Membrane and Surface Immobilized Glucose Oxidase". Anal. Chem. 64 (17): 1795-1804. doi: ...
... and urea-induced unfolding of the dimeric enzyme glucose oxidase". Biochemistry. 41 (11): 3819-27. doi:10.1021/bi0116700. PMID ...
The extract has also shown significant inhibition against glucose oxidase-mediated inflammation. The observations have ...
Roth JP, Klinman JP (January 2003). "Catalysis of electron transfer during activation of O2 by the flavoprotein glucose oxidase ... Leys D, Basran J, Scrutton NS (August 2003). "Channelling and formation of 'active' formaldehyde in dimethylglycine oxidase". ...
The Glucose oxidase of Penicillium amagasakiense has been studied in detail because of the use of Glucose oxidase in biosensors ... Zoldak, G.; Zubrik, A.; Musatov, A.; Stupak, M.; Sedlak, E. (2004). "Irreversible Thermal Denaturation of Glucose Oxidase from ... MycoBank ATCC UniProt Conserved arginine-516 of Penicillium amagasakiense glucose oxidase isessential for the efficient binding ... Courjean, O.; Mano, N. (2011). "Recombinant glucose oxidase from Penicillium amagasakiense for efficient bioelectrochemical ...
Abbreviations CHOD = cholesterol oxidase GOD = glucose oxidase GPO = glycerol-3-phosphate oxidase PAP = phenol + aminophenazone ... water Glucose (GOD-Perid method) Glucose + oxygen + water --(enzyme glucose oxidase)--> gluconate + hydrogen peroxide Hydrogen ... Cholesterol (CHOD-PAP method) Cholesterol + oxygen --(enzyme cholesterol oxidase)--> cholestenone + hydrogen peroxide Hydrogen ... enzyme glycerol-3-phosphate oxidase) --> dihydroxyacetone phosphate + hydrogen peroxide Hydrogen peroxide + 4-aminophenazone + ...
Enzymes used in ELISAs include horseradish peroxidase (HRP), alkaline phosphatase (AP) or glucose oxidase. These enzymes allow ...
In commercial baking, the use of glucose oxidase enzyme (GOD) enhances texture, size, and loaf form. A. tubingensis is part of ... glucose oxidase, phytase, xylanase, acid phosphatase and xylosidase production. Amylase produced by A. tubingensis has ... "The developmental evaluation of Aspergillus tubingensis CTM 507 glucose oxidase toxicity in Wistar rats". Journal of Food ...
Enz-RAFT uses 1-4 μM glucose oxidase to remove dissolved oxygen from the system. As the degassing is decoupled from the ...
2008). "Aerobic Fermentation of D-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain". Appl. Environ. ... The number of glucose sensor genes have remained mostly consistent through the budding yeast lineage, however glucose sensors ... When grown in glucose-rich media, trypanosomatid parasites degrade glucose via aerobic fermentation. In this group, this ... One group developed the ECOM3 (E. coli cytochrome oxidase mutant) strain by removing three terminal cytochrome oxidases (cydAB ...
"Crosslinking of wheat dough proteins by glucose oxidase and the resulting effects on bread and croissants". Food Chemistry. 89 ...
"Tobacco nectarin V is a flavin-containing berberine bridge enzyme-like protein with glucose oxidase activity". Plant Physiology ... a covalently flavinylated oxidase of benzophenanthridine alkaloid biosynthesis in plants". The Journal of Biological Chemistry ...
It is an example of a biosensor-based test for glucose. This is similar to the glucose oxidase assay, as the glucose in the ... Clinistrip consists of a section that contains glucose oxidase that has been dried onto the paper pad. ... The intensity of the colour change on the pad reflects the amount of glucose present in the urine. v t e. ... Clinistrip, also known as CLINISTIX or the brand name Diastix, are specific clinical sticks for the detection of glucose. ...
The oxidases xanthine oxidase, aldehyde oxidase, and sulfite oxidase[31] Legumes, whole grains, nuts[25] molybdenum deficiency ... Involved in glucose and lipid metabolism, although its mechanisms of action in the body and the amounts needed for optimal ... Required component of many redox enzymes, including cytochrome c oxidase Liver, seafood, oysters, nuts, seeds; some: whole ...
Glucose-6-phosphate isomerase (GPI; PGI, PHI, AMF). *Glia maturation factor (GMF) ...
The enzyme is involved in carbohydrate metabolism, glucose metabolism, the uronate cycle and may play a role in the water ... Xanthine oxidase. 1.1.4: disulfide as acceptor. *Vitamin K epoxide reductase. *Vitamin-K-epoxide reductase (warfarin- ...
... glucose derivative] must fit like a lock and key, in order to be able to exert a chemical effect on each other.) ... "Infrared evidence of cyanide binding to iron and copper sites in bovine heart cytochrome c oxidase. Implications regarding ...
UDP-glucuronic acid is formed when UDP-glucose undergoes two oxidations catalyzed by the enzyme UDP-glucose 6-dehydrogenase. ... l-gulonolactone oxidase, the last of the chain of four enzymes that synthesize vitamin C.[183][184] American biochemist Irwin ... It is transported through the intestine via both glucose-sensitive and glucose-insensitive mechanisms, so the presence of large ... l-gulonolactone oxidase (GULO), that catalyses the last step in the biosynthesis, is highly mutated and non-functional.[117][ ...
UDP-glucuronic acid is formed when UDP-glucose undergoes two oxidations catalyzed by the enzyme UDP-glucose 6-dehydrogenase. ... He later developed the theory that humans possess a mutated form of the L-gulonolactone oxidase coding gene.[185] ... It is transported through the intestine via both glucose-sensitive and glucose-insensitive mechanisms, so the presence of large ... SVCT1 and SVCT2 import the reduced form of ascorbate across plasma membranes.[102] GLUT1 and GLUT3 are glucose transporters, ...
Glucose oxidase. *L-gulonolactone oxidase. *Xanthine oxidase. 1.1.4: disulfide as acceptor. *Vitamin K epoxide reductase ...
It also participates in glucose metabolism and osmoregulation and plays a protective role against toxic aldehydes derived from ... Xanthine oxidase. 1.1.4: disulfide as acceptor. *Vitamin K epoxide reductase. *Vitamin-K-epoxide reductase (warfarin- ... Under diabetic conditions AR converts glucose into sorbitol, which is then converted to fructose. 20466987 It has been found to ...
Glucose oxidase - antioksidan. *Glucose syrup - tatlandırıcı. *Glutamat -. *Glutamik asid - aroma arttırıcı. *Gluten - ...
The oxidation of -SH groups in the bacterial cytoplasmic membrane results in loss of the ability to transport glucose and also ... Rada B, Leto TL (2008). "Oxidative innate immune defenses by Nox/Duox family NADPH Oxidases". Contrib Microbiol. Contributions ... Rada B, Leto TL (2009). "Redox warfare between airway epithelial cells and Pseudomonas: Dual oxidase versus pyocyanin". Immunol ... Rada B, Leto TL (2009). "Redox warfare between airway epithelial cells and Pseudomonas: Dual oxidase versus pyocyanin". Immunol ...
Their function is to convert the potential energy of glucose, amino acids, and fatty acids into adenosine triphosphate (ATP) in ... cytochrome c oxidase (COX), cause most cases of Leigh disease. Mutations in a gene called SURF1 (surfeit1) are the most common ... though the mutations in cytochrome c oxidase and other electron transport chain proteins were not discovered until 1977.[6] ... all of whom had a severe deficiency in cytochrome c oxidase (COX), the fourth complex in the mitochondrial electron transport ...
A higher glucose concentration results in more Hb A1c. Because the reaction is slow, the Hb A1c proportion represents glucose ... they are under intense oxidative stress caused by the production of hydrogen peroxide via autoxidation and/or monoamine oxidase ... Because of the slow rate of Hb A combination with glucose, the Hb A1c percentage reflects a weighted average of blood glucose ... The binding of glucose to amino acids in the hemoglobin takes place spontaneously (without the help of an enzyme) in many ...
cytochroom-c-oxidase Ja Externe links[bewerken , brontekst bewerken]. *Integrated Enzyme Database (European Bioinformatics ... glucose 1-dehydrogenase Ja 1.1.1.49 glucose-6-phosphate dehydrogenase Ja 1.1.1.50 3alpha-hydroxysteroid dehydrogenase (B- ...
... and maintenance of normal blood glucose concentrations (ID 4244) pursuant to Article 13(1) of Regulation (EC) No 1924/2006". ... Monoamine oxidase inhibitors. Hidden categories: *Articles containing Ancient Greek-language text. *Articles containing ...
Quelle des Wasserstoffperoxids ist vielfach die Reaktion von Glucose mit Sauerstoff in Gegenwart des Enzyms Glucose-Oxidase (EC ... Immunotoxins containing glucose oxidase and lactoperoxidase with tumoricidal properties: in vitro killing effectiveness in a ... So werden Enzyme wie Lysozym, Lactoperoxidase und Glucose-Oxidase von den Zahnpasten auf das Pellikel übertragen. Als ... und Glucose-Oxidase enthalten, das Lactoperoxidase-System aktivieren, sind aus jüngster Zeit Studien bekannt, die den ...
If a person has an arterial catheter, arterial blood is recommended for blood glucose testing.[6] ... multiple organ failure ensues because tissues are unable to use oxygen efficiently due to inhibition of cytochrome c oxidase.[ ... Glucose levels taken from capillary blood should be interpreted with care because such measurements may not be accurate. ... "Blood glucose control in patients with severe sepsis and septic shock". World Journal of Gastroenterology. 15 (33): 4132-6. ...
Cytochrome oxidase Ferrous or Ferric. Catalase. Cytochrome (via Heme). Nitrogenase. Hydrogenase Magnesium. Glucose 6- ...
Xanthine oxidase inhibitors decrease the production of uric acid, by interfering with xanthine oxidase. Uricosurics increase ... Medications most often used to treat hyperuricemia are of two kinds: xanthine oxidase inhibitors and uricosurics. ...
Reactive hydrogen peroxide (H2O2), the byproduct of oxalate oxidase reaction, can be neutralized by catalase. Alarm ... Thus, the basic reaction by which photosynthesis is used to produce food (such as glucose) was outlined.[74] ... such as glucose. In other bacteria, different mechanisms such as the reverse Krebs cycle are used to achieve the same end. ... oxalate released from calcium oxalate crystals is converted to CO2 by an oxalate oxidase enzyme and the produced CO2 can ...
In arabica green coffee beans, the content of free glucose was 30 to 38 mg/100g, free fructose 23 to 30 mg/100g; free galactose ... Other proteins include enzymes, such as catalase and polyphenol oxidase, which are important for the maturation of green coffee ...
An example of the latter is combined entrapment of glucose oxidase and insulin in a pH-responsive hydrogel. In the presence of ... involving the use of similar types of smart polymers that can detect changes in blood glucose levels and trigger production or ... glucose, the formation of gluconic acid by the enzyme triggers release of insulin from the hydrogel. ...
This mechanism allows tumorous cells to convert the majority of their glucose stores into lactate regardless of oxygen ... Xanthine oxidase. 1.1.4: disulfide as acceptor. *Vitamin K epoxide reductase. *Vitamin-K-epoxide reductase (warfarin- ... fasting individual who has been drinking and is dependent on gluconeogenesis to maintain blood glucose levels. Alanine and ... availability, shifting use of glucose metabolites from simple energy production to the promotion of accelerated cell growth and ...
... phosphate oxidase[3] which also catalyzes the conversion of PNP to PLP. Pyridoxine 5′-phosphate oxidase is dependent on flavin ... Glucose metabolism[edit]. PLP is a required coenzyme of glycogen phosphorylase, the enzyme necessary for glycogenolysis to ... Its active form, pyridoxal 5′-phosphate, serves as a coenzyme in some 100 enzyme reactions in amino acid, glucose, and lipid ... so deprivation of vitamin B6 results in impaired glucose tolerance.[4] ...
Some of the amino acids are convertible (with the expenditure of energy) to glucose and can be used for energy production, just ... Copper required component of many redox enzymes, including cytochrome c oxidase Main article: Copper in health ... This may help lower blood glucose levels because it can slow the absorption of sugar. Additionally, fiber, perhaps especially ... Traditionally, simple carbohydrates are believed to be absorbed quickly, and therefore to raise blood-glucose levels more ...
cellular response to glucose stimulus. • phthalate metabolic process. • locomotory behavior. • response to light stimulus. • ...
... increases blood glucose to a lesser extent than sucrose (thus having a relatively low glycemic index[16]) so is used ... Eventually, it yields a syrup containing about 42% fructose, 52% glucose, and 6% maltose. Sucrose is simply hydrolyzed into an ... glucose) can produce yields 200 g (7.1 oz) mannitol per liter of feedstock. Further research is being conducted, studying ways ... convert either three fructose molecules or two fructose and one glucose molecule into two mannitol molecules, and one molecule ...
Oxidative/fermentation glucose test. *Phenylalanine deaminase test. *Reverse CAMP test. *Salt tolerance test ...
Glúcós Glucose. *Ocsaíodáis glúcós Glucose oxidase. *Glútan Gluten. *Aigéad glútamach Glutamic acid ...
... species also typically give a positive result to the oxidase test, the absence of gas formation from glucose, ... Oxidase-positive. Other characteristics that tend to be associated with Pseudomonas species (with some exceptions) include ... glucose is oxidised in oxidation/fermentation test using Hugh and Leifson O/F test, beta hemolytic (on blood agar), indole ...
Glucose oxidase. *L-gulonolactone oxidase. *Xanthine oxidase. *Alcohol oxidase. 1.1.4: disulfide as acceptor. *Vitamin K ...
In the glucose oxidase assay, the glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen ... The glucose oxidase binds specifically to β-D-glucopyranose and does not act on α-D-glucose. It oxidises all of the glucose in ... "Glucose Oxidase: A much used and much loved enzyme in biosensors" at University of Paisley Glucose+Oxidase at the US National ... Glucose oxidase catalyzes the oxidation of β-D-glucose into D-glucono-1,5-lactone, which then hydrolyzes into gluconic acid. In ...
The bulk of dietary carbohydrate is absorbed mainly as glucose in the intestine. Glucose is the major... ... The sugar glucose is an aldohexose and is the most important carbohydrate present in our body. ... Kumar V., Gill K.D. (2018) Estimation of Blood Glucose Levels by Glucose Oxidase Method. In: Basic Concepts in Clinical ... High levels of glucose are observed in diabetes mellitus, while decrease in glucose levels occurs in starvation and ...
Products containing GLUCOSE OXIDASE made by company: Aubrey Organics, Inc. There are no products in the database that match ...
Definition of glucose oxidase paper strip test. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms ... glucose oxidase paper strip test. Definition: a qualitative test for glucose in the urine, in which glucose is oxidized to ... gluconic acid by glucose oxidase; a specific test, unless ascorbic acid is present. ...
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Oxidase, glucose EC Number:. 232-601-0. EC Name:. Oxidase, glucose. CAS Number:. 9001-37-0 Molecular formula:. Not applicable, ... Active enzyme protein of Glucose oxidase (EC no. 232-601-0, CAS no. 9001-37-0, EC name: Glucose oxidase, Enzyme Class no. 1.1. ... Active enzyme protein of Glucose oxidase (EC no. 232-601-0, CAS no. 9001-37-0, EC name: Glucose oxidase, Enzyme Class no. 1.1. ... Active enzyme protein of Glucose oxidase (EC no. 232-601-0, CAS no. 9001-37-0, EC name: Glucose oxidase, Enzyme Class no. 1.1. ...
... particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The ... Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface ... biodetectors may be employed for mediatorless detection of a wide variety of analytes depending on the oxidase employed. ... Other oxidases, such as alcohol oxidase, galactose oxidase, lactic acid oxidase, amino acid oxidase, cholesterol oxidase, ...
References for Abcams Anti-Glucose Oxidase antibody (ab34679). Please let us know if you have used this product in your ...
Skin Care , Medicating , Anti-itch/rash Cream containing GLUCOSE OXIDASE There are no products in the database that match your ...
1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for ... PROTEIN (GLUCOSE OXIDASE) A 583 Aspergillus niger EC#: 1.1.3.4 IUBMB Gene Name(s): gox ...
Drug: Glucose oxidase + glucose Isotone saline + 200U/ml of glucose oxidase + 5% of glucose in a bag on valve nasal spray ... Active Comparator: Glucose oxidase + glucose A nasal spray (bag-on-valve device) with 200U/ml glucose oxidase + 5% glucose in ... After this the persons spray with glucose oxidase+glucose/only saline+glucose 5 times the first day and thereafter 3 times ... glucose oxidase+saline and glucose or only saline+glucose). The outcome measure is predominantly calculated as the sum of all ...
... Authors: X. Meng, X. Ren and F. Tang ... Keywords: biosensor, nanoparticles, glucose oxidase. Abstract:. Biosensors have a wide field of potential applications in ...
Abcam provides specific protocols for Anti-Glucose Oxidase antibody (ab34679) : Immunoprecipitation protocols, Western blot ...
Drug: Glucose oxidase + glucose Isotone saline + 200U/ml of glucose oxidase + 5% of glucose in a bag on valve nasal spray ... Active Comparator: Glucose oxidase + glucose A nasal spray (bag-on-valve device) with 200U/ml glucose oxidase + 5% glucose in ... After this the persons spray with glucose oxidase+glucose/only saline+glucose 5 times the first day and thereafter 3 times ... Glucose Oxidase as Treatment Against Common Cold. The safety and scientific validity of this study is the responsibility of the ...
Potentiometric Measurement of Glucose Concentration with an Immobilized Glucose Oxidase/Catalase Electrode. ... Potentiometric Measurement of Glucose Concentration with an Immobilized Glucose Oxidase/Catalase Electrode ... A series of enzyme electrodes for measurement of glucose have been constructed. The electrodes contain glucose oxidase ... Potentiometric Measurement of Glucose Concentration with an Immobilized Glucose Oxidase/Catalase Electrode ...
Glucose oxidase is a synthetic enzyme that catalyzes the oxidation of the sugar glucose to gluconic acid. It functions as a ...
... Author(s). Witteveen, C.F.B.; Veenhuis, M.; ...
It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has ... It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen ... An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and ... antibacterial activity in the presence of glucose and oxygen. ... Glucose Oxidase. Subscribe to New Research on Glucose Oxidase ...
M. Sedeek, G. Callera, A. Montezano et al., "Critical role of Nox4-based NADPH oxidase in glucose-induced oxidative stress in ... MicroRNA-146a Decreases High Glucose/Thrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression. Huang- ... T. Ago, T. Kitazono, H. Ooboshi et al., "Nox4 as the major catalytic component of an endothelial NAD(P)H oxidase," Circulation ... S. Lee, N. M. Gharavi, H. Honda et al., "A role for NADPH oxidase 4 in the activation of vascular endothelial cells by oxidized ...
Both L-glucose treatment and L-glucose/thrombin cotreatment had no discernible effects on the mRNA expression of Nox1, Nox2, ... M. Sedeek, G. Callera, A. Montezano et al., "Critical role of Nox4-based NADPH oxidase in glucose-induced oxidative stress in ... However, it is unknown whether a combination of high glucose and thrombin can modulate the expression of NAPDH oxidase (Nox) ... Thrombin at a dose of 2 units/mL and D-glucose (25 mmol/L) were added to the HAECs for the respective times. L-glucose (25 mmol ...
A combination of the glucose oxidase-diaminobenzidine (DAB) method and the DAB-nickel method can successfully bring out details ... The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system Neurosci Lett. 1988 Feb 29;85(2):169- ... A combination of the glucose oxidase-diaminobenzidine (DAB) method and the DAB-nickel method can successfully bring out details ...
Laboratory testing for CAS number 9001-37-0. beta-d-glucose : oxygen 1-oxidoreductase, beta-d-glucose oxidase,. This chemical ... Glucose oxidase):. L-glutamate oxidase Glucose Acetobromo-a-d-glucose Oxidase, l-amino acid Ascorbate oxidase l(-)-glucose ... Glucose oxidase - CAS # 9001-37-0. Information provided on Glucose oxidase (9001-37-0) is for reference only and is subject to ... Chemical Name: Glucose oxidase. CAS Number: 9001-37-0. EC Number (EINECS): 232-601-0. Synonyms: beta-d-glucose : oxygen 1- ...
... we demonstrate Glucose oxidase (GOx) mediated targeted cancer-starving therapy by self-assembled vesicle of trimesic acid based ... Glucose Oxidase Mediated Targeted Cancer-Starving Therapy by Biotinylated Self-Assembled Vesicle S. Dinda, S. Sarkar and P. K. ... Herein, we demonstrate Glucose oxidase (GOx) mediated targeted cancer-starving therapy by self-assembled vesicle of trimesic ... Glucose Oxidase Mediated Targeted Cancer-Starving Therapy by Biotinylated Self-Assembled Vesicle ...
... glucose (CAS NO.9001-37-0) ,Find latest products of Oxidase, glucose manufacturers, suppliers, exporters and producers on ... Glucose, d-glucose, deoxy glucose, View all oxidase, glucose, D(+)-Glucose, d-glucose, deoxy glucose, b-glucose, glucose ... D-Glucoseoxidase. D-Glucose-1-oxidase. De-oxin. Deoxin-1. E.C. 1.1.3.4. Fermizyme GO1500. Fermizyme GO-10000. Glucose 1-oxidase ... Home -, Hot Product Listed O -, Oxidase, glucose Related Searches: oxidase, glucose, D(+)- ...
We developed two glucose biosensors based on poly(3,4-ethylenedioxythiophene) (PEDOT) and polyacrylic acid (PAA) doped with ... First (reaction 1), the glucose present in the solution reacts with glucose oxidase. During the process, glucose becomes ... G. Liu, Y. Lin, Amperometric glucose biosensor based on self-assembling glucose oxidase on carbon nanotubes. Electrochem. ... Enzymatic Assay of glucose oxidase EC 1.1.3.4) by detection of hydrogen peroxide created in enzymatic oxidation of glucose. The ...
... ,, Previous Message , Next Message ,, From:. Mark & Carrie Byrne ,[email protected], (by way ...
A major source of ROS in endothelial cells exposed to glucose is the NAD(P)H oxidase enzyme. Several studies demonstrated that ... C-peptide reduces high-glucose-induced apoptosis of endothelial cells and decreases NAD(P)H-oxidase reactive oxygen species ... We hypothesised that C-peptide reduces glucose-induced ROS generation by decreasing NAD(P)H oxidase activation and prevents ... Glucose-induced ROS production was quenched by C-peptide and this was associated with a decreased NAD(P)H oxidase activity and ...
A glucose oxidase (GO) has been identified in the ejaculatory duct of male Drosophila melanogaster. Evidence is given that this ... A glucose oxidase (GO) has been identified in the ejaculatory duct of male Drosophila melanogaster. Evidence is given that this ... Genetics of male-specific glucose oxidase and the identification of other unusual hexose enzymes in Drosophila melanogaster ... These are NAD(P)-glucose dehydrogenase (GODH) and NAD-gluconate dehydrogenase (GNDH). GODH and GNDH are found in both males and ...
To confirm this materials electrochemical activity, a glucose oxidase (GOD) is chosen as the target reagent to modify the rGON ... In addition, this GOD/rGON-AuNP/GC electrode shows catalytic activity toward glucose oxidation reaction. ... glucose oxidase; direct electron transfer reduced graphene oxide nanosheets; gold nanoparticles; composite materials; glucose ... To confirm this materials electrochemical activity, a glucose oxidase (GOD) is chosen as the target reagent to modify the rGON ...
  • The glucose oxidase enzyme (GOx or GOD) also known as notatin (EC number 1.1.3.4) is an oxidoreductase that catalyses the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. (wikipedia.org)
  • Several species of fungi and insects synthesize glucose oxidase, which produces hydrogen peroxide, which kills bacteria. (wikipedia.org)
  • Early experiments showed that notatin exhibits in vitro antibacterial activity (in the presence of glucose) due to hydrogen peroxide formation. (wikipedia.org)
  • citation needed] In the glucose oxidase assay, the glucose is first oxidized, catalyzed by glucose oxidase, to produce gluconate and hydrogen peroxide. (wikipedia.org)
  • Glucose oxidase is a hydrogen peroxide producing enzyme, which also is present in honey. (clinicaltrials.gov)
  • Glucose oxidase is a dimeric protein that catalyzes the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolyzed to gluconic acid. (abcam.com)
  • It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (curehunter.com)
  • Further progress was made when Guilbault and Lubrano [ 3 ] determined glucose in blood on the basis of amperometric monitoring of the liberated hydrogen peroxide. (springer.com)
  • C. Song, P. E. Pehrsson and W. Zhao, "Optical Enzymatic Detection of Glucose Based on Hydrogen Peroxide-Sensitive HiPco Carbon Nanotubes," Journal of Materials Research, Vol. 21, No. 11, 2006, pp. 2817-2823. (scirp.org)
  • This was modelled by a system of partial differential equations and boundary conditions representing convective and diffusive transport of the substrates glucose and hydrogen peroxide, as well as reaction kinetics of the bienzyme electrode. (dit.ie)
  • An enzyme which converts glucose into gluconic acid and hydrogen peroxide (H2O2). (bioscience.ws)
  • if the glucose is present, the hydrogen peroxide produced in the reaction can be detected by reacting it with an indicator to change the color of the urine. (bioscience.ws)
  • The performance of the biosensors was evaluated in terms of linear response range for hydrogen peroxide and glucose, detection limit, and susceptibility to some. (lu.se)
  • The operational stability of the biosensors was evaluated by applying a steady potential of -50 mV versus Ag/AgCl to the glucose biosensor and injecting standard solutions of hydrogen peroxide and glucose (50 muM and 1.0 mM, respectively, in phosphate buffer) for at least 5 h in a flow-injection system. (lu.se)
  • Glucose oxidase catalyses the oxidation of glucose to hydrogen peroxide. (sleepingbearfarms.com)
  • Glucose oxidase catalyzes the reaction by which sugar creates hydrogen peroxide and gluconolactone. (sleepingbearfarms.com)
  • This reaction uses up the glucose (sugar) and protects against bacteria via the production of hydrogen peroxide. (sleepingbearfarms.com)
  • The antimicrobial activity in most honeys is due to the enzymatic production of hydrogen peroxide - which is facilitated by glucose oxidase. (sleepingbearfarms.com)
  • Uses dissolved molecular oxygen to break down glucose into hydrogen peroxide and gluconic acid. (bio-cat.com)
  • a highly specific method for measurement of glucose in serum or plasma by reaction with glucose oxidase, in which gluconic acid and hydrogen peroxide are formed. (thefreedictionary.com)
  • GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. (preprints.org)
  • iKnowZyme GO can transfer glucose into gluconic acid with oxygen and produce hydrogen peroxide at the same time. (iknowzyme.com)
  • With oxidase mimicking, hydrogen peroxide is generated from the coupled photocatalysis of glucose oxidation and dioxygen reduction under visible-light irradiation with a near 100% apparent quantum. (supercaramels.com)
  • Introduction of Challen GOX (Glucose Oxidase)Glucose oxidase catalyzes the reaction of glucose with oxygen to form gluconic acid and hydrogen peroxide, which has a significant antibacterial and bactericidal effect. (worldenzyme.com)
  • Glucose oxidase can inhibit the growth and reproduction of harmful bacteria in the intestinal tract of animals by consuming oxygen, producing gluconic acid and strong oxidizing substance hydrogen peroxide, improving the intestinal health of the animal, thereby reducing the problems of diarrhea, overfeeding and enteritis problems. (worldenzyme.com)
  • Glucose is oxidized by glucose oxidase to gluconate and hydrogen peroxide. (pointescientific.com)
  • Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. (emdmillipore.com)
  • In the presence of glucose, the glucose oxidase produced hydrogen peroxide, which killed off the bacteria. (wikipedia.org)
  • Localization of glucose oxidase and catalase activities in Aspergillus niger. (wur.nl)
  • A gene of glucose oxidase (GOD) from Aspergillus niger Z-25 was cloned and sequenced. (dtu.dk)
  • Singh, OV 2006, ' Mutagenesis and analysis of mold Aspergillus niger for extracellular glucose oxidase production using sugarcane molasses ', Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology , vol. 135, no. 1, pp. 43-58. (elsevier.com)
  • Singh, O. V. / Mutagenesis and analysis of mold Aspergillus niger for extracellular glucose oxidase production using sugarcane molasses . (elsevier.com)
  • Glucose oxidase (GOX) from Aspergillus niger is a very much portrayed. (supercaramels.com)
  • To demonstrate the role of glucose oxidase (GOD) on the enzyme-catalyzed production of xylose from SCB hemicellulose, a variant of the GOD gene, which contains two amino acid substitutions, was cloned from Aspergillus niger CICC40179. (usda.gov)
  • Glucose Oxidase, Aspergillus niger, Recombinant, CAS 9001-37-0, oxidizes β-D-glucose to D-glucono-ω-lactone and H2O2 and converts D-glucono-ω-lactone to gluconic acid. (emdmillipore.com)
  • Glucose oxidase synthesis by Aspergillus niger GIV-10 on starch. (dimensions.ai)
  • He eventually found that these bacteria could only thrive adjacent to Aspergillus niger if glucose was not present. (wikipedia.org)
  • 12. The bioelectrode of claim 1 further comprising depositing at least two oxidase enzymes atop the deposited monolayer-coated colloidal gold peroxidase layer. (google.com)
  • The biosensor is based on a bi-enzyme channelling configuration, employing the enzymes glucose oxidase and horseradish peroxidase, with direct electron transfer of horseradish peroxidase at a conducting polymer electrode. (dit.ie)
  • The comparative kinetic study of two glucose oxidizing enzymes, FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase, is presented in the artificial electron transfer mediator system based on ruthenium(III) compounds. (ebscohost.com)
  • PQQ-dependent glucose dehydrogenase is more active than FAD-dependent glucose oxidase in the process of D-glucose oxidation by ruthenium(III) compounds, this being due to the different catalytic mechanisms of these enzymes. (ebscohost.com)
  • In addition, the use of catalase combined with glucose oxidase provided the best result, decreasing the glucose concentration by 51% and 78% after 24 and 48 h, respectively, when 200 U/ml of both enzymes was used. (ajevonline.org)
  • Here we describe the immobilisation of two biocatalytically relevant co-factor recycling enzymes, glucose dehydrogenase (GDH) and NADH oxidase (NOD) on aldehyde functional ReSynTM polymer microspheres with varying functional group densities. (csir.co.za)
  • Like other enzymes, the amount of glucose oxidase in raw honey can vary depending on the flowers and nectar that go into the honey. (sleepingbearfarms.com)
  • One of those enzymes is glucose oxidase, which reacts with glucose to form a molecule. (supercaramels.com)
  • abstract = "Labial glands of Helicoverpa zea produced a glucose oxidase (GOX), which was present in the saliva and midgut lumen. (elsevier.com)
  • abstract = "We have immobilized a glucose oxidase (GOD) in mesoporous gels derived from tetraethoxysilane (TEOS) and organosilanes (i.e., methyltriethoxysilane (MTES), vinyltriethoxysilane (VTES), propyltriethoxysilane (PTES), and phenyltriethoxysilane (PhTES)) through a two-step sol-gel process without any templating agent. (elsevier.com)
  • Enzymatic glucose biosensors use an electrode instead of O2 to take up the electrons needed to oxidize glucose and produce an electronic current in proportion to glucose concentration. (wikipedia.org)
  • The CL detection involved enzymatic oxidation of glucose to d-gluconic acid and H(2)O(2), and then the generated H(2)O(2) oxidizing luminol to produce CL emission in the presence of HRP. (biomedsearch.com)
  • An effective enzymatic glucose biosensor was developed by immobilizing glucose oxidase on chitosan submicron particles synthesized from the gladius of Todarodes pacificus (GCSP). (edu.au)
  • In the present work, the use of the enzymatic system glucose oxidase/catalase was evaluated as an alternative to decrease the glucose concentration and eventually produce a reduced alcohol wine. (ajevonline.org)
  • The use of glucose oxidase as a generator of H 2 O 2 in the enzymatic radioiodination of components of cell surfaces. (semanticscholar.org)
  • Plasma glucose, cholesterol and triglyceride contents were measured using the glucose oxidase method (Lott and Turner, 1975), the enzymatic (cholesterol oxidase) and colorimetric method (Patsch et al. (thefreedictionary.com)
  • 1976), and the enzymatic (glycerol phosphate oxidase) and colorimetric (PAP) method (Nagele et al. (thefreedictionary.com)
  • This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH-211, if maltooligosaccharide or modified starch is used as a substrate) in clinical analysis. (sorachim.com)
  • D-glucono-δ-Lactone + H2O2This enzyme is used for enzymatic determination of glucose, and for amylase-activity assay when coupled with α-glucosidase (AGH-201, AGH- 211, if maltooligosaccharide or modified starch is used as a. (sorachim.com)
  • Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: fabrication, characterization, and enzymatic activity. (semanticscholar.org)
  • The glucose sensor took advantage of the enzymatic. (supercaramels.com)
  • Useful for enzymatic assay of glucose concentration. (emdmillipore.com)
  • Useful for enzymatic assay of glucose. (emdmillipore.com)
  • The electrodes contain glucose oxidase immobilized on platinum, either with or without co-immobilization of catalase. (diabetesjournals.org)
  • The effects of glucose oxidase, catalase and aeration on the glucose concentration were evaluated after 24 and 48 h of treatment of 27 °Brix Carmenere must. (ajevonline.org)
  • A major drawback was obtained due to the color change of Carmenere must because of the H 2 O 2 that was produced during the glucose oxidase treatment, despite the presence of catalase. (ajevonline.org)
  • It was possible to obtain a lower alcoholic degree using the glucose oxidase/catalase system, but if the goal is the industrial application of this technique, the color change must be investigated further. (ajevonline.org)
  • An evaluation of the glucose oxidase/catalase ratio was projected to show an improvement of the H 2 O 2 elimination and subsequently decrease the effect on color change. (ajevonline.org)
  • These are NAD(P)-glucose dehydrogenase (GODH) and NAD-gluconate dehydrogenase (GNDH). (nih.gov)
  • The glucose level in haemolymph of exposed snails was elevated while the glycogen, pro-tein content and the activities of hexokinase (HK), pyruvatekinase (PK) and lactate dehydrogenase (LDH) showed a decrease in soft tissues when compared with the control group. (scirp.org)
  • It is demonstrated that FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase follow Michaelis kinetics in the D-glucose/ruthenium(III) system. (ebscohost.com)
  • Glucose-dehydrogenase. (sorachim.com)
  • Glucose-dehydrogenase (FAD-dependent) from MicroorganismD-Glucose:(flavine adenine dinucleotide)-dehydrogenaseEC # 1.1.99.10If you need bigger quantities than indicated on the web site, please contact us for a personal offer. (sorachim.com)
  • Dong L, Yu W, Liu M, Liu Y, Shao Q, Li A, Yan W, Zhang J. Novel Composite Electrode of the Reduced Graphene Oxide Nanosheets with Gold Nanoparticles Modified by Glucose Oxidase for Electrochemical Reactions. (mdpi.com)
  • Glucose oxidase (GOD), horseradish peroxidase (HRP) and gold nanoparticles were immobilized with sol-gel method on the inside surface of the CL flow cell. (biomedsearch.com)
  • It was found that gold nanoparticles could remarkably enhance the CL respond of the glucose biosensor. (biomedsearch.com)
  • Glucose oxidase (GOx) has been exploited as a reducing as well as a stabilising agent for the green chemical synthesis of Au nanoparticles at pH 7.0 under ambient conditions. (rsc.org)
  • Amperometric glucose biosensor was fabricated by immobilizing glucose oxidase (GOx) onto chitosan nanoparticles (CSNPs) in gold electrode. (edu.au)
  • Glucose oxidase was covalently bound to the magnetic nanoparticles by modifying the surfaces with 3-(aminopropyl)triethoxysilane (APTES) and a common protein crosslinking agent, glutaraldehyde. (megazyme.com)
  • An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. (curehunter.com)
  • It is a flavoprotein, highly specific for beta-D-glucose. (curehunter.com)
  • Electrosynthetic poly(phenol) nanofilms were deposited in situ on platinum electrodes in the presence and absence of glucose oxidase. (dcu.ie)
  • which is essential to perform redox reactions in the absence of glucose oxidase, is eliminated, resulting in a lower-cost and more compact device. (supercaramels.com)
  • 4. The bioelectrode of claim 1 wherein the oxidase is cholesterol oxidase, xanthine oxidase, amino acid oxidase, lactic acid oxidase, alcohol oxidase, glycolate oxidase, pyridoxal-4-oxidase, sorbose oxidase, gulonolactose oxidase or galactose oxidase. (google.com)
  • Glucose Oxidase Assay Kit ab138884 uses a quick and sensitive fluorometric assay for the measurement of glucose oxidase activity. (abcam.com)
  • A series of enzyme electrodes for measurement of glucose have been constructed. (diabetesjournals.org)
  • Glucose oxidase is an oxidoreductase exhibiting a high β-D-glucose specificity and high stability which renders glucose oxidase well-suited for applications in diabetes care. (openaire.eu)
  • Oxidoreductase that catalyzes the conversion of D-glucose to D-glucono-1,5-lactone which hydrolyzes spontanously to gluconate. (roche.com)
  • The absorbance at 505 nm is proportional to concentration of glucose in the sample. (wikipedia.org)
  • The immobilization technique, some of the environmental variables such as oxygen concentration and pH, and several compounds that might interfere with the selectivity of the enzyme electrodes for glucose have received preliminary study. (diabetesjournals.org)
  • This direct potentiometric approach is undergoing further evaluation to determine the basic electrochemical mechanism responsible for the potentiometric signal and whether it can be adapted for continuous in vivo monitoring of the glucose concentration in body fluids. (diabetesjournals.org)
  • The presented method was successfully applied for determination of glucose concentration in food samples, such as grape juice and honey. (springer.com)
  • The CL emission intensity was linear with glucose concentration in the range of 1.0 x 10(-5)molL(-1) to 1.0 x 10(-3)molL(-1), and the detection limit was 5 x 10(-6)molL(-1) (3sigma). (biomedsearch.com)
  • The time needed to complete this configuration change decreases with the concentration of added glucose. (eurekamag.com)
  • After a 1 h incubation, the glucose concentration in the medium was determined by the glucose oxidase method (Sigma GAGO 20 test kit), according to instructions. (thefreedictionary.com)
  • The rate of LbL film decomposition increased with the glucose concentration. (preprints.org)
  • At pH and ionic strength close to physiological conditions, it was possible to slowly decompose the LbL film at a sub-millimolar glucose concentration. (preprints.org)
  • The catalytic activity of GOx towards the oxidation of glucose was taken advantage of to form a Au nanoparticle-polyaniline (Au NP-PANI) composite at room temperature. (rsc.org)
  • The glucose oxidase enzyme (GOx) also known as notatin (EC number 1.1.3.4) is an oxido-reductase that catalyses the oxidation of glucose to hydrogen. (supercaramels.com)
  • It is a flavoprotein, which catalyses the oxidation of glucose. (biovenproducts.com)
  • Effective immobilization of glucose oxidase on chitosan submicron part" by J R. Anusha, Albin T. Fleming et al. (edu.au)
  • Immobilization of glucose oxidase onto g. (metu.edu.tr)
  • The properties of a glucose biosensor made by immobilization of glucose oxidase onto gelatin in a layer of electrochemically deposited polyaniline have been investigated. (metu.edu.tr)
  • Direct Electrochemistry and Immobilization of Glucose Oxidase on Nano-Carbon Aerogels[J].Acta Phys. (pku.edu.cn)
  • This enzyme is produced by certain species of fungi and insects and displays antibacterial activity when oxygen and glucose are present. (wikipedia.org)
  • It is also used as a food preservative to help remove oxygen and glucose from food when packaged such as dry egg powder to prevent unwanted browning and undesired taste. (wikipedia.org)
  • It is widely used for the determination of glucose in body fluids and in removing residual glucose and oxygen from beverages, food and other agricultural products. (abcam.com)
  • The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. (curehunter.com)
  • Their first device consisted of enzyme glucose oxidase (GOD) entrapped over an oxygen electrode via a semipermeable dialysis membrane. (springer.com)
  • C-peptide reduces high-glucose-induced apoptosis of endothelial cells and decreases NAD(P)H-oxidase reactive oxygen species generation in human aor. (nih.gov)
  • Trinder, P. (1969) Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. (scirp.org)
  • The cyclic voltammetry measurements revealed that both the electrodes exhibit high electrocatalytic ability towards glucose detection in the presence of oxygen. (edu.au)
  • The enzyme causes an electrochemical reaction in the presence of glucose and oxygen, generating an electrical signal. (supercaramels.com)
  • Glucose and oxygen from the surrounding tissue diffuse to the sensor, where the enzyme glucose oxidase carries. (supercaramels.com)
  • Glucose oxidase can consume molecular oxygen or atomic oxygen, oxidize glucose, and protect oxidizable components in feed. (worldenzyme.com)
  • Nevertheless, GOx activity is highly oxygen dependent which can lead to inaccuracies in amperometric β-D-glucose determinations. (openaire.eu)
  • Amperometric measurements were carried out for the determination of glucose in phosphate buffer. (springer.com)
  • The proposed biosensor exhibited short response time, easy operation, low cost and simple assembly, and the proposed biosensor was successfully applied to the determination of glucose in human serum. (biomedsearch.com)
  • For the quantitative determination of Glucose in serum on the Tokyo Boeki Medisys Inc. Biolis 24i analyzer. (pointescientific.com)
  • The glucose oxidase biosensor was developed by forming a polyaniline-deposited electrode surface as support for the immobilized enzyme gel, in order to increase its durability. (metu.edu.tr)
  • It oxidises all of the glucose in solution because the equilibrium between the α and β anomers is driven towards the β side as it is consumed in the reaction. (wikipedia.org)
  • Glucose oxidase is widely used coupled to peroxidase reaction that visualizes colorimetrically the formed H2O2, for the determination of free glucose in sera or blood plasma for diagnostics, using spectrometric assays manually or with automated procedures, and even point-of-use rapid assays. (wikipedia.org)
  • In addition, this GOD/rGON-AuNP/GC electrode shows catalytic activity toward glucose oxidation reaction. (mdpi.com)
  • X. W. Shen, C. Z. Huang and Y. F. Li, "Localized Surface Plasmon Resonance Sensing Detection of Glucose in the Serum Samples of Diabetes Sufferers Based on the Redox Reaction of Chlorauric Acid," Talanta, Vol. 72, No. 4, 2007, pp. 1432-1437. (scirp.org)
  • MastersThesis{Solgun2020thesis, AUTHOR = "SOLGUN, Bager Baris", TITLE = "Study of dynamics of the glucose oxidase-glucose-ferricyanide-NaOH reaction in a continuous stirred tank reactor [online]", YEAR = "2020 [cit. (theses.cz)
  • Background: Glucose oxidase (GoxA) catalyzes the reaction from β-D-glucose to gluconic acid. (currentbiotechnology.com)
  • Studies have shown that glucose oxidase can enhance the animal's liver redox reaction in two ways to reduce the harm of mycotoxins to the animal body, and has a significant effect on shortening the sow estrus cycle and improving the reproductive health of poultry. (worldenzyme.com)
  • A nasal spray from a bag-on-valve device that mixes glucose oxidase with glucose has undergone clinical trials in 2016 for the prevention and treatment of the common cold. (wikipedia.org)
  • Is it possible to shorten an episode of common cold with a nasal spray containing glucose oxidase? (clinicaltrials.gov)
  • We are using this protocol at start, before the persons in the study start their treatment and thereafter every day for the next 7 days, while they use the nasal spray(glucose oxidase+saline and glucose or only saline+glucose). (clinicaltrials.gov)
  • In the present study we aim to investigate if a nasal spray with glucose oxidase could treat a common cold, when the treatment is started even after the onset of the symptoms. (clinicaltrials.gov)
  • A nasal spray with isotone saline + 5% glucose in a bag-on-valve nasal spray device. (clinicaltrials.gov)
  • an oxidase layer atop the deposited colloidal gold adsorbed peroxidase wherein direct electron transfer to the conducting surface occurs in the presence of an analyte when the bioelectrode is suitably coupled with a reference/counter electrode. (google.com)
  • An optimised glucose oxidase bioelectrode exhibiting high performance direct electron transfer. (surrey.ac.uk)
  • A glucose oxidase (GOd) bioelectrode exhibiting high performance, direct electron transfer (DET) has been prepared. (surrey.ac.uk)
  • Glucose oxidase (GOx) has been developed as glucose sensor for measuring blood glucose level because of its specificity to glucose oxidation. (supercaramels.com)
  • However, it is unknown whether a combination of high glucose and thrombin can modulate the expression of NAPDH oxidase (Nox) subtypes in human aortic endothelial cells (HAECs). (hindawi.com)
  • High glucose increases oxidative stress in endothelial cells and induces apoptosis. (nih.gov)
  • A major source of ROS in endothelial cells exposed to glucose is the NAD(P)H oxidase enzyme. (nih.gov)
  • Human aortic endothelial cells (HAEC) were exposed to 25 mmol/l glucose in the presence or absence of C-peptide and tested for protein quantity and activity of caspase-3 and other apoptosis markers by ELISA, TUNEL and immunoblotting. (nih.gov)
  • In glucose-exposed endothelial cells, C-peptide acts as an endogenous antioxidant molecule by reducing RAC-1 translocation to membrane and NAD(P)H oxidase activation. (nih.gov)
  • By preventing oxidative stress, C-peptide protects endothelial cells from glucose-induced apoptosis. (nih.gov)
  • Rat retinal endothelial cells (RRECs) were grown in normal (N) or HG (30 mM glucose) medium for 7 days. (arvojournals.org)
  • 13 - 15 Our recent study suggests that lysyl oxidase (LOX), a cross-linking enzyme critical for the development and maturation of the BM, is upregulated in rat retinal endothelial cells (RRECs) grown in high-glucose (HG) medium and in diabetic rat retinas. (arvojournals.org)
  • The aim of this study was in silico and in vivo analysis of SPs effect on the production of recombinant glucose oxidase (GOX) in Yarrowia lipolytica . (springer.com)
  • 9. A bioelectrode for mediatorless electrochemical detection of glucose comprising a conducting surface having a first deposited layer of monolayer-coated colloidal gold-adsorbed horseradish peroxidase and a second deposited layer of colloidal gold-adsorbed glucose oxidase atop said first layer. (google.com)
  • 11. The bioelectrode of claim 9 wherein the first layer of colloidal gold-adsorbed horseradish peroxidase is evaporatively deposited onto the electrode surface prior to evaporative deposition of the second layer of colloidal gold-adsorbed glucose oxidase. (google.com)
  • In particular, mediatorless detection of glucose is possible with colloidal gold adsorbed horseradish peroxidase in the presence of glucose oxidase. (google.com)
  • Chemiluminescence flow biosensor for glucose based on gold nanoparticle-enhanced activities of glucose oxidase and horseradish peroxidase. (biomedsearch.com)
  • Optimising the ratio of horseradish peroxidase and glucose oxidase on " by Dana Mackey, Anthony Killard et al. (dit.ie)
  • Diet factors responsible for the change of the glucose oxidase activity in labial salivary glands of Helicoverpa armigera. (docme.ru)
  • Salivary glucose oxidase: Multifunctional roles for Helicoverpa zea? (elsevier.com)
  • Fingerprint Dive into the research topics of 'Salivary glucose oxidase: Multifunctional roles for Helicoverpa zea? (elsevier.com)
  • This is the technology behind the disposable glucose sensor strips used by diabetics to monitor serum glucose levels. (wikipedia.org)
  • The sample was centrifuged and serum as stored at -80AdegC for blood assays of glucose by glucose oxidase method , serum insulin by ELISA and calculation of HOMA-IR. (thefreedictionary.com)
  • Serum glucose level (mmol/L) was determined using glucose test kit based on the glucose oxidase method (Dialab Glucose, GOD-PAP, Austria), as described [24]. (thefreedictionary.com)
  • This enzyme is widely used in making biosensors to detect the glucose levels in blood or serum. (biovenproducts.com)
  • the phallic Saunders advances his advent of glucose oxidase test in urine the firmament pseudonymously. (91xz.tk)
  • blowhard danglings that territorially glucose oxidase test in urine driveling? (91xz.tk)
  • Dresden Lucas humidifying, glucose oxidase test in urine she did it very well, please. (91xz.tk)
  • Alston's floricultural glucose oxidase test in urine fold, she allows it very disgusting. (91xz.tk)
  • Desolate, Garv objects to his truncated and letter glucose oxidase test in urine bomb from now on! (91xz.tk)
  • Centennial form glucose oxidase test in urine that vitriolize qualitatively? (91xz.tk)
  • The Glucose Oxidase Assay Kit (ab138884) is for the use in one 96- or one 384-well microplate. (abcam.com)
  • The Glucose Oxidase assay kit is a simple procedure for the rapid and reliable measurement and analysis of glucose oxidase activity in industrial enzyme preparations and bread improver mixtures. (megazyme.com)
  • Glucose Oxidase assay kit is suitable for manual, auto-analyser and microplate formats. (megazyme.com)
  • The electrochemical glucose sensing properties of CSNPs based electrode (Au/CSNPs/GOx) was analyzed relatively with commercial chitosan particles utilized electrode (Au/CS/GOx). (edu.au)
  • The most important ROS-generating systems in the vasculature include the mitochondrial electron transport chain, xanthine oxidase, and NADPH oxidases (Noxes) [ 2 ]. (hindawi.com)
  • Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. (google.com)
  • In addition, the very low value of Michaelis-Menten constant for GCSP based electrode contributes a better affinity of the electrode surface towards glucose oxidase. (edu.au)
  • Flavoproteins can function as hydrophobic sites for vitamin B_2 (riboflavin) or, in other structures, with cofactors for catalytic reactions such as glucose oxidation. (caltech.edu)
  • M. Morikawa, N. Kimizuka, M. Yoshihara and T. Endo, "New Colorimetric Detection of Glucose by Means of Electronaccepting Indicators: Ligand Substitution of [Fe(acac)3-n(phen)n]n+ Complexes Triggered by Electron Transfer from Glucose Oxidase," Chemistry-A European Journal, Vol. 8, No. 24, 2002, pp. 5580-5584. (scirp.org)
  • A novel electron-transfer mediator consisting of monosubstituted 4-hydroxycyclobut-3-ene-1,2-dione (squarate) was used as a mediator for Pt/poly(phenol) nano-film/GOx amperometric glucose biosensors. (dcu.ie)
  • More than 50 years have passed since Clark and Lyons [ 1 ] first proposed the concept of the glucose enzyme electrode. (springer.com)
  • The biodetectors may be employed for mediatorless detection of a wide variety of analytes depending on the oxidase employed. (google.com)
  • Linear range for the detection of glucose was from 0.5 × 10 -6 M to 12 × 10 -6 M while detection limit was 6.26 × 10 -8 M (S/N = 3). (scirp.org)
  • J. Kremeskotter, R. Wilson and D. J. Schiffrin, "Detection of Glucose via Electrochemilumine-Science in a Thin-Layer Cell with a Planar Optical Waveguide," Measurement Science and Technology, Vol. 6, No. 9, 1995, pp. 1325-1328. (scirp.org)
  • The developed biosensors possessed good amperometric response towards glucose with linear detection range from 0.001 to 1 mM with ≤2 s response time. (edu.au)
  • The response time of the glucose oxidase biosensor is 90 s, the detection limit is below 1 mmol/dm(3) and the sensor can be used 20 times within a 2-month period without losing its stability. (metu.edu.tr)
  • Kumar V., Gill K.D. (2018) Estimation of Blood Glucose Levels by Glucose Oxidase Method. (springer.com)
  • Patients and methods A commercial glucometer, which is a plasma separation GO-based glucometer, was employed to compare the accuracy of the commercial blood glucometer with that of a standard glucose analyser for monitoring blood glucose levels in premature infants. (bmj.com)
  • Membrane-associated semicarbazide-sensitive amine oxidase (SSAO) is mainly present in the media of aorta and in adipose tissue. (aspetjournals.org)
  • The report on NADPH Oxidase 4 Market offers in-depth analysis of market trends, drivers, restraints, opportunities etc. (medicalmarketreport.com)
  • We showed that high glucose (HG) exerted a synergistic effect with thrombin to induce a 10.69-fold increase in Nox4 mRNA level in HAECs. (hindawi.com)
  • To investigate the effect of reducing high glucose (HG)-induced lysyl oxidase (LOX) overexpression and increased activity on retinal endothelial cell apoptosis. (arvojournals.org)
  • Glucose oxidase is an enzyme naturally produced by some fungi and insects ( Wong, assays developed for research use (Berron et al. (supercaramels.com)
  • Based on electric conductivity and wide potential window of ionic liquid (IL) and electric property of single-wall car- bon nanotubes (SWCNTs), composite material of IL-SWCNTs was prepared, glucose sensor was built with this mate-rial for immobilizing glucose oxidase (GOx). (scirp.org)
  • In this paper, we present a CuO-based glucose sensor. (supercaramels.com)
  • We use inkjet-printing technology for the rapid and low-cost deposition of all the components of this glucose sensor, from the electronics to the biorecognition elements, on commercially available. (supercaramels.com)
  • 3. The bioelectrode of claim 1 wherein colloidal gold adsorbed oxidase is deposited atop the peroxidase. (google.com)
  • When placed in buffered glucose, the enzyme electrodes show a potentiometric response to glucose with respect to a Ag/AgCl reference electrode. (diabetesjournals.org)
  • The prepared electrodes functioned as first-generation glucose biosensors at applied potential E = + 0.6 V during t = 30 s. (springer.com)
  • S. R. Lee, Y. T. Lee, K. Sawada, H. Takao and M. Ishida, "Development of a Disposable Glucose Biosensor Using Electroless-Plated Au/Ni/Copper Low Electrical Resistance Electrodes," Biosensors and Bioelectronics, Vol. 24, No. 3, 2008, pp. 410-414. (scirp.org)
  • Glucose biosensors based on lyophilised, crystalline and cross-linked glucose oxidase (GOx, CLEC(R)) and commercially available lyophilised GOx immobilised on top of glassy carbon electrodes modified with electrodeposited Prussian Blue are critically compared. (lu.se)
  • Glucose oxidase (GOD)/CA/ glassy carbon (GC) electrodes were prepared by immobilizing GOD on the surface of nano-carbon aerogels. (pku.edu.cn)
  • Similar assays allows the monitoring of glucose levels in fermentation, bioreactors, and to control glucose in vegetal raw material and food products. (wikipedia.org)
  • Glucose oxidase is fermented by the improved strains using a liquid submerged fermentation technique, and produced by an advanced post-treatment technique. (bestzyme.com)
  • FACZYME GOD5 is Glucose Oxidase that developed by Finely animal care through high technical fermentation procedure. (faczyme.com)
  • I need to be able to diplay/animate Glucose Oxidase (1gpe), highlight FAD within the enzyme, remove FAD, and show a version of the molecule that is now missing FAD. (scripps.edu)
  • On the basis of types, Glucose and Maltodextrin market is segmented into - Glucose Maltodextrin - On the basis of applications, Glucose and Maltodextrin. (supercaramels.com)
  • The global glucose oxidase market is segmented on the basis of Type, Application, and geography. (medicalmarketreport.com)
  • Due to the significant antibacterial and bactericidal action of glucose oxidase, Challen GOX can reduce the amount of antibiotics in the feed and make a green feed. (worldenzyme.com)
  • We developed two glucose biosensors based on poly(3,4-ethylenedioxythiophene) (PEDOT) and polyacrylic acid (PAA) doped with poly(4-lithium styrenesulfonic acid) (PSSLi) or PEDOT and anthranilic acid (AA) doped with poly(4-styrenesulfonic acid) (PSSH). (springer.com)
  • Amperometric glucose biosensor based on glucose oxidase immobilized ov" by J R. Anusha, C Justin Raj et al. (edu.au)
  • Arterial blood samples were collected and analysed, including samples adjusted for specific glucose (3.6 mmol/L, 8.3 mmol/L and 16.7 mmol/L) and PaO 2 levels (range, 40-400 mm Hg). (bmj.com)
  • One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0. (emdmillipore.com)