Genetic Vectors
Insect Vectors
Disease Vectors
Gene Transfer Techniques
Transduction, Genetic
Adenoviridae
Dependovirus
Lentivirus
Arthropod Vectors
Support Vector Machines
Retroviridae
Base Sequence
Transfection
Molecular Sequence Data
Cloning, Molecular
Gene Expression
Green Fluorescent Proteins
Genetic Engineering
Arachnid Vectors
Helper Viruses
Aedes
Mosquito Control
Promoter Regions, Genetic
Adenovirus E1 Proteins
beta-Galactosidase
Insect Control
Luminescent Proteins
Culicidae
Genes, Reporter
Anopheles gambiae
Culex
Recombination, Genetic
Escherichia coli
Adenoviruses, Human
Insecticides
Recombinant Fusion Proteins
DNA, Recombinant
Malaria
Amino Acid Sequence
Virus Integration
Gene Targeting
Lac Operon
Transformation, Genetic
Virus Replication
Polymerase Chain Reaction
Psychodidae
Phlebotomus
Cells, Cultured
Mice, Inbred BALB C
Ceratopogonidae
Tumor Cells, Cultured
Vaccines, Synthetic
DNA Primers
Defective Viruses
Mice, Inbred C57BL
Kanamycin Kinase
Gene Expression Regulation
Moloney murine leukemia virus
DNA
Transcription, Genetic
Pyrethrins
Mutation
Restriction Mapping
Hemiptera
RNA, Messenger
Coxsackie and Adenovirus Receptor-Like Membrane Protein
Adenoviruses, Simian
HeLa Cells
Artificial Intelligence
Simuliidae
Leukemia Virus, Murine
DNA, Complementary
Thymidine Kinase
Terminal Repeat Sequences
Transformation, Bacterial
Spumavirus
Sequence Analysis, DNA
Larva
Gene Expression Regulation, Viral
Algorithms
Dengue
Chagas Disease
CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/23054)
Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors. (+info)C-myc overexpression and p53 loss cooperate to promote genomic instability. (2/23054)
p53 monitors genomic integrity at the G1 and G2/M cell cycle checkpoints. Cells lacking p53 may show gene amplification as well as the polyploidy or aneuploidy typical of many tumors. The pathways through which this develops, however, are not well defined. We demonstrate here that the combination of p53 inactivation and c-myc overexpression in diploid cells markedly accelerates the spontaneous development of tetraploidy. This is not seen with either N-myc or L-myc. Tetraploidy is accompanied by significantly higher levels of cyclin B and its associated cdc2 kinase activity. Mitotic spindle poisons accelerate the appearance of tetraploidy in cells either lacking functional p53 or overexpressing c-myc whereas the combination is additive. Restoration of p53 function in cells overexpressing c-myc causing rapid apoptosis, indicating that cells yet to become tetraploid have nonetheless suffered irreversible genomic and/or mitotic spindle damage. In the face of normal p53 function, such damage would either be repaired or trigger apoptotis. We propose that loss of p53 and overexpression of c-myc permits the emergence and survival of cells with increasingly severe damage and the eventual development of tetraploidy. (+info)Telomerase activity is sufficient to allow transformed cells to escape from crisis. (3/23054)
The introduction of simian virus 40 large T antigen (SVLT) into human primary cells enables them to proliferate beyond their normal replicative life span. In most cases, this temporary escape from senescence eventually ends in a second proliferative block known as "crisis," during which the cells cease growing or die. Rare immortalization events in which cells escape crisis are frequently correlated with the presence of telomerase activity. We tested the hypothesis that telomerase activation is the critical step in the immortalization process by studying the effects of telomerase activity in two mortal SVLT-Rasval12-transformed human pancreatic cell lines, TRM-6 and betalox5. The telomerase catalytic subunit, hTRT, was introduced into late-passage cells via retroviral gene transfer. Telomerase activity was successfully induced in infected cells, as demonstrated by a telomerase repeat amplification protocol assay. In each of nine independent infections, telomerase-positive cells formed rapidly dividing cell lines while control cells entered crisis. Telomere lengths initially increased, but telomeres were then maintained at their new lengths for at least 20 population doublings. These results demonstrate that telomerase activity is sufficient to enable transformed cells to escape crisis and that telomere elongation in these cells occurs in a tightly regulated manner. (+info)Stabilization of poly-L-lysine/DNA polyplexes for in vivo gene delivery to the liver. (4/23054)
We are developing a self-assembling non-viral in vivo gene delivery vehicle based on poly-l-lysine and plasmid DNA. We have characterized poly-l-lysines of different chain lengths for DNA condensation and strength of DNA binding. Poly-l-lysine chains >20 residues bound DNA efficiently in physiological saline, while shorter chains did not. Attachment of asialoorosomucoid to PLL increased the PLL chain length required for efficient DNA binding in saline and for efficient DNA condensation. By electron microscopy, poly-l-lysine/DNA polyplexes appeared as toroids 25-50 nm in diameter or rods 40-80 nm long; conjugation of asialoorosomucoid to the polylysine component increased the size of resulting polyplexes to 50-90 nm. In water, poly-l-lysine and asialoorosomucoid-PLL polyplexes have effective diameters of 46 and 87.6 nm, respectively. Polyplexes containing only poly-l-lysine and DNA aggregated in physiological saline at all charge ratios and aggregated at neutral charge ratios in water. Attachment of asialoorosomucoid lessened, but did not eliminate, the aggregation of PLL polyplexes, and did not result in efficient delivery of polyplexes to hepatocytes. Conjugation of polyethylene glycol to poly-l-lysine sterically stabilized resulting polyplexes at neutral charge ratios by shielding the surfaces. For efficient in vivo gene delivery, polyplexes will need to be sterically stabilized to prevent aggregation and interaction with serum components. (+info)Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (5/23054)
Herpes simplex virus type 1 (HSV-1) has many attractive features that suggest its utility for gene transfer to neurons. However, viral cytotoxicity and transient transgene expression limit practical applications even in the absence of viral replication. Mutant viruses deleted for the immediate early (IE) gene, ICP4, an essential transcriptional transactivator, are toxic to many cell types in culture in which only the remaining IE genes are expressed. In order to test directly the toxicity of other IE gene products in neurons and develop a mutant background capable of longterm transgene expression, we generated mutants deleted for multiple IE genes in various combinations and tested their relative cytotoxicity in 9L rat gliosarcoma cells, Vero monkey kidney cells, and primary rat cortical and dorsal root neurons in culture. Viral mutants deleted simultaneously for the IE genes encoding ICP4, ICP22 and ICP27 showed substantially reduced cytotoxicity compared with viruses deleted for ICP4 alone or ICP4 in combination with either ICP22, ICP27 or ICP47. Infection of neurons in culture with these triple IE deletion mutants substantially enhanced cell survival and permitted transgene expression for over 21 days. Such mutants may prove useful for efficient gene transfer and extended transgene expression in neurons in vitro and in vivo. (+info)Inducible long-term gene expression in brain with adeno-associated virus gene transfer. (6/23054)
Recombinant adeno-associated virus (rAAV) vectors hold promise for treating a number of neurological disorders due to the ability to deliver long-term gene expression without toxicity or immune response. Critical to these endeavors will be controlled expression of the therapeutic gene in target cells. We have constructed and tested a dual cassette rAAV vector carrying a reporter gene under the control of the tetracycline-responsive system and the tetracycline transactivator. Transduction in vitro resulted in stable expression from the vector that can be suppressed 20-fold by tetracycline treatment. In vivo experiments, carried out to 6 weeks, demonstrated that vector-transduced expression is sustained until doxycycline administration upon which reporter gene expression is reduced. Moreover, the suppression of vector-driven expression can be reversed by removal of the drug. These studies demonstrate long-term regulated gene expression from rAAV vectors. This system will provide a valuable approach for controlling vector gene expression both in vitro and in vivo. (+info)Adenoviral gene transfer into the normal and injured spinal cord: enhanced transgene stability by combined administration of temperature-sensitive virus and transient immune blockade. (7/23054)
This study characterized gene transfer into both normal and injured adult rat dorsal spinal cord using first (E1-/E3-) or second (E1-/E2A125/E3-, temperature-sensitive; ts) generation of replication-defective adenoviral (Ad) vectors. A novel immunosuppressive regimen aimed at blocking CD4/CD45 lymphocytic receptors was tested for improving transgene persistence. In addition, the effect of gene transfer on nociception was also evaluated. Seven days after treatment, numerous LacZ-positive cells were observed after transfection with either viral vector. By 21 days after transfection, beta-galactosidase staining was reduced and suggestive of ongoing cytopathology in both Ad-treated groups, despite the fact that the immunogenicity of LacZ/Adts appeared less when compared with that elicited by the LacZ/Ad vector. In contrast, immunosuppressed animals showed a significant (P < or = 0.05) increase in the number of LacZ-positive cells not displaying cytopathology. In these animals, a concomitant reduction in numbers of macrophages/microglia and CD4 and CD8 lymphocytes was observed. Only animals that received LacZ/Adts and immunosuppression showed transgene expression after 60 days. Similar results were observed in animals in which the L4-L5 dorsal roots were lesioned before transfection. Gene transfer into the dorsal spinal cord did not affect nociception, independent of the adenovirus vector. These results indicate that immune blockade of the CD4/CD45 lymphocytic receptors enhanced transgene stability in adult animals with normal or injured spinal cords and that persistent transgene expression in the spinal cord does not interfere with normal neural function. (+info)Antitumor effect of allogenic fibroblasts engineered to express Fas ligand (FasL). (8/23054)
Fas ligand is a type II transmembrane protein which can induce apoptosis in Fas-expressing cells. Recent reports indicate that expression of FasL in transplanted cells may cause graft rejection and, on the other hand, tumor cells may lose their tumorigenicity when they are engineered to express FasL. These effects could be related to recruitment of neutrophils by FasL with activation of their cytotoxic machinery. In this study we investigated the antitumor effect of allogenic fibroblasts engineered to express FasL. Fibroblasts engineered to express FasL (PA317/FasL) did not exert toxic effects on transformed liver cell line (BNL) or colon cancer cell line (CT26) in vitro, but they could abrogate their tumorigenicity in vivo. Histological examination of the site of implantation of BNL cells mixed with PA317/FasL revealed massive infiltration of polymorphonuclear neutrophils and mononuclear cells. A specific immune protective effect was observed in animals primed with a mixture of BNL or CT26 and PA317/FasL cells. Rechallenge with tumor cells 14 or 100 days after priming resulted in protection of 100 or 50% of animals, respectively. This protective effect was due to CD8+ cells since depletion of CD8+ led to tumor formation. In addition, treatment of pre-established BNL tumors with a subcutaneous injection of BNL and PA317/FasL cell mixture at a distant site caused significant inhibition of tumor growth. These data demonstrate that allogenic cells engineered with FasL are able to abolish tumor growth and induce specific protective immunity when they are mixed with neoplastic cells. (+info)A genetic vector is a vehicle, often a plasmid or a virus, that is used to introduce foreign DNA into a host cell as part of genetic engineering or gene therapy techniques. The vector contains the desired gene or genes, along with regulatory elements such as promoters and enhancers, which are needed for the expression of the gene in the target cells.
The choice of vector depends on several factors, including the size of the DNA to be inserted, the type of cell to be targeted, and the efficiency of uptake and expression required. Commonly used vectors include plasmids, adenoviruses, retroviruses, and lentiviruses.
Plasmids are small circular DNA molecules that can replicate independently in bacteria. They are often used as cloning vectors to amplify and manipulate DNA fragments. Adenoviruses are double-stranded DNA viruses that infect a wide range of host cells, including human cells. They are commonly used as gene therapy vectors because they can efficiently transfer genes into both dividing and non-dividing cells.
Retroviruses and lentiviruses are RNA viruses that integrate their genetic material into the host cell's genome. This allows for stable expression of the transgene over time. Lentiviruses, a subclass of retroviruses, have the advantage of being able to infect non-dividing cells, making them useful for gene therapy applications in post-mitotic tissues such as neurons and muscle cells.
Overall, genetic vectors play a crucial role in modern molecular biology and medicine, enabling researchers to study gene function, develop new therapies, and modify organisms for various purposes.
Insect vectors are insects that transmit disease-causing pathogens (such as viruses, bacteria, parasites) from one host to another. They do this while feeding on the host's blood or tissues. The insects themselves are not infected by the pathogen but act as mechanical carriers that pass it on during their bite. Examples of diseases spread by insect vectors include malaria (transmitted by mosquitoes), Lyme disease (transmitted by ticks), and plague (transmitted by fleas). Proper prevention measures, such as using insect repellent and reducing standing water where mosquitoes breed, can help reduce the risk of contracting these diseases.
A disease vector is a living organism that transmits infectious pathogens from one host to another. These vectors can include mosquitoes, ticks, fleas, and other arthropods that carry viruses, bacteria, parasites, or other disease-causing agents. The vector becomes infected with the pathogen after biting an infected host, and then transmits the infection to another host through its saliva or feces during a subsequent blood meal.
Disease vectors are of particular concern in public health because they can spread diseases rapidly and efficiently, often over large geographic areas. Controlling vector-borne diseases requires a multifaceted approach that includes reducing vector populations, preventing bites, and developing vaccines or treatments for the associated diseases.
Gene transfer techniques, also known as gene therapy, refer to medical procedures where genetic material is introduced into an individual's cells or tissues to treat or prevent diseases. This can be achieved through various methods:
1. **Viral Vectors**: The most common method uses modified viruses, such as adenoviruses, retroviruses, or lentiviruses, to carry the therapeutic gene into the target cells. The virus infects the cell and inserts the new gene into the cell's DNA.
2. **Non-Viral Vectors**: These include methods like electroporation (using electric fields to create pores in the cell membrane), gene guns (shooting gold particles coated with DNA into cells), or liposomes (tiny fatty bubbles that can enclose DNA).
3. **Direct Injection**: In some cases, the therapeutic gene can be directly injected into a specific tissue or organ.
The goal of gene transfer techniques is to supplement or replace a faulty gene with a healthy one, thereby correcting the genetic disorder. However, these techniques are still largely experimental and have their own set of challenges, including potential immune responses, issues with accurate targeting, and risks of mutations or cancer development.
Genetic transduction is a process in molecular biology that describes the transfer of genetic material from one bacterium to another by a viral vector called a bacteriophage (or phage). In this process, the phage infects one bacterium and incorporates a portion of the bacterial DNA into its own genetic material. When the phage then infects a second bacterium, it can transfer the incorporated bacterial DNA to the new host. This can result in the horizontal gene transfer (HGT) of traits such as antibiotic resistance or virulence factors between bacteria.
There are two main types of transduction: generalized and specialized. In generalized transduction, any portion of the bacterial genome can be packaged into the phage particle, leading to a random assortment of genetic material being transferred. In specialized transduction, only specific genes near the site where the phage integrates into the bacterial chromosome are consistently transferred.
It's important to note that genetic transduction is not to be confused with transformation or conjugation, which are other mechanisms of HGT in bacteria.
Adenoviridae is a family of viruses that includes many species that can cause various types of illnesses in humans and animals. These viruses are non-enveloped, meaning they do not have a lipid membrane, and have an icosahedral symmetry with a diameter of approximately 70-90 nanometers.
The genome of Adenoviridae is composed of double-stranded DNA, which contains linear chromosomes ranging from 26 to 45 kilobases in length. The family is divided into five genera: Mastadenovirus, Aviadenovirus, Atadenovirus, Siadenovirus, and Ichtadenovirus.
Human adenoviruses are classified under the genus Mastadenovirus and can cause a wide range of illnesses, including respiratory infections, conjunctivitis, gastroenteritis, and upper respiratory tract infections. Some serotypes have also been associated with more severe diseases such as hemorrhagic cystitis, hepatitis, and meningoencephalitis.
Adenoviruses are highly contagious and can be transmitted through respiratory droplets, fecal-oral route, or by contact with contaminated surfaces. They can also be spread through contaminated water sources. Infections caused by adenoviruses are usually self-limiting, but severe cases may require hospitalization and supportive care.
A dependovirus, also known as a dependent adenovirus or satellite adenovirus, is a type of virus that requires the presence of another virus, specifically an adenovirus, to replicate. Dependoviruses are small, non-enveloped viruses with a double-stranded DNA genome. They cannot complete their replication cycle without the help of an adenovirus, which provides necessary functions for the dependovirus to replicate.
Dependoviruses are clinically significant because they can cause disease in humans, particularly in individuals with weakened immune systems. In some cases, dependoviruses may also affect the severity and outcome of adenovirus infections. However, it is important to note that not all adenovirus infections are associated with dependovirus co-infections.
A lentivirus is a type of slow-acting retrovirus that can cause chronic diseases and cancers. The term "lentivirus" comes from the Latin word "lentus," which means slow. Lentiviruses are characterized by their ability to establish a persistent infection, during which they continuously produce new viral particles.
Lentiviruses have a complex genome that includes several accessory genes, in addition to the typical gag, pol, and env genes found in all retroviruses. These accessory genes play important roles in regulating the virus's replication cycle and evading the host's immune response.
One of the most well-known lentiviruses is the human immunodeficiency virus (HIV), which causes AIDS. Other examples include the feline immunodeficiency virus (FIV) and the simian immunodeficiency virus (SIV). Lentiviruses have also been used as vectors for gene therapy, as they can efficiently introduce new genes into both dividing and non-dividing cells.
Arthropod vectors are living organisms, specifically arthropods such as mosquitoes, ticks, fleas, and lice, that can transmit infectious agents (such as viruses, bacteria, or parasites) from one host to another. This process is called vector-borne transmission. The arthropod vectors become infected with the pathogen while taking a blood meal from an infected host, then transmit the pathogen to another host during subsequent feedings. The transmission can occur through various means, including biting, stinging, or even mechanical contact. It's important to note that not all arthropods are vectors, and only certain species within each group are capable of transmitting diseases.
Support Vector Machines (SVM) is not a medical term, but a concept in machine learning, a branch of artificial intelligence. SVM is used in various fields including medicine for data analysis and pattern recognition. Here's a brief explanation of SVM:
Support Vector Machines is a supervised learning algorithm which analyzes data and recognizes patterns, used for classification and regression analysis. The goal of SVM is to find the optimal boundary or hyperplane that separates data into different classes with the maximum margin. This margin is the distance between the hyperplane and the nearest data points, also known as support vectors. By finding this optimal boundary, SVM can effectively classify new data points.
In the context of medical research, SVM has been used for various applications such as:
* Classifying medical images (e.g., distinguishing between cancerous and non-cancerous tissues)
* Predicting patient outcomes based on clinical or genetic data
* Identifying biomarkers associated with diseases
* Analyzing electronic health records to predict disease risk or treatment response
Therefore, while SVM is not a medical term per se, it is an important tool in the field of medical informatics and bioinformatics.
A transgene is a segment of DNA that has been artificially transferred from one organism to another, typically between different species, to introduce a new trait or characteristic. The term "transgene" specifically refers to the genetic material that has been transferred and has become integrated into the host organism's genome. This technology is often used in genetic engineering and biomedical research, including the development of genetically modified organisms (GMOs) for agricultural purposes or the creation of animal models for studying human diseases.
Transgenes can be created using various techniques, such as molecular cloning, where a desired gene is isolated, manipulated, and then inserted into a vector (a small DNA molecule, such as a plasmid) that can efficiently enter the host organism's cells. Once inside the cell, the transgene can integrate into the host genome, allowing for the expression of the new trait in the resulting transgenic organism.
It is important to note that while transgenes can provide valuable insights and benefits in research and agriculture, their use and release into the environment are subjects of ongoing debate due to concerns about potential ecological impacts and human health risks.
'Anopheles' is a genus of mosquitoes that are known for their role in transmitting malaria parasites to humans. These mosquitoes have a distinctive resting posture, with their abdomens raised and heads down, and they typically feed on human hosts at night. Only female Anopheles mosquitoes transmit the malaria parasite, as they require blood meals to lay eggs.
There are over 400 species of Anopheles mosquitoes worldwide, but only about 30-40 of these are considered significant vectors of human malaria. The distribution and behavior of these mosquitoes can vary widely depending on the specific species and geographic location.
Preventing and controlling the spread of malaria involves a variety of strategies, including the use of insecticide-treated bed nets, indoor residual spraying, antimalarial drugs, and vaccines. Public health efforts to reduce the burden of malaria have made significant progress in recent decades, but the disease remains a major global health challenge, particularly in sub-Saharan Africa.
Retroviridae is a family of viruses that includes human immunodeficiency virus (HIV) and other viruses that primarily use RNA as their genetic material. The name "retrovirus" comes from the fact that these viruses reverse transcribe their RNA genome into DNA, which then becomes integrated into the host cell's genome. This is a unique characteristic of retroviruses, as most other viruses use DNA as their genetic material.
Retroviruses can cause a variety of diseases in animals and humans, including cancer, neurological disorders, and immunodeficiency syndromes like AIDS. They have a lipid membrane envelope that contains glycoprotein spikes, which allow them to attach to and enter host cells. Once inside the host cell, the viral RNA is reverse transcribed into DNA by the enzyme reverse transcriptase, which is then integrated into the host genome by the enzyme integrase.
Retroviruses can remain dormant in the host genome for extended periods of time, and may be reactivated under certain conditions to produce new viral particles. This ability to integrate into the host genome has also made retroviruses useful tools in molecular biology, where they are used as vectors for gene therapy and other genetic manipulations.
A base sequence in the context of molecular biology refers to the specific order of nucleotides in a DNA or RNA molecule. In DNA, these nucleotides are adenine (A), guanine (G), cytosine (C), and thymine (T). In RNA, uracil (U) takes the place of thymine. The base sequence contains genetic information that is transcribed into RNA and ultimately translated into proteins. It is the exact order of these bases that determines the genetic code and thus the function of the DNA or RNA molecule.
Transfection is a term used in molecular biology that refers to the process of deliberately introducing foreign genetic material (DNA, RNA or artificial gene constructs) into cells. This is typically done using chemical or physical methods, such as lipofection or electroporation. Transfection is widely used in research and medical settings for various purposes, including studying gene function, producing proteins, developing gene therapies, and creating genetically modified organisms. It's important to note that transfection is different from transduction, which is the process of introducing genetic material into cells using viruses as vectors.
Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.
Molecular cloning is a laboratory technique used to create multiple copies of a specific DNA sequence. This process involves several steps:
1. Isolation: The first step in molecular cloning is to isolate the DNA sequence of interest from the rest of the genomic DNA. This can be done using various methods such as PCR (polymerase chain reaction), restriction enzymes, or hybridization.
2. Vector construction: Once the DNA sequence of interest has been isolated, it must be inserted into a vector, which is a small circular DNA molecule that can replicate independently in a host cell. Common vectors used in molecular cloning include plasmids and phages.
3. Transformation: The constructed vector is then introduced into a host cell, usually a bacterial or yeast cell, through a process called transformation. This can be done using various methods such as electroporation or chemical transformation.
4. Selection: After transformation, the host cells are grown in selective media that allow only those cells containing the vector to grow. This ensures that the DNA sequence of interest has been successfully cloned into the vector.
5. Amplification: Once the host cells have been selected, they can be grown in large quantities to amplify the number of copies of the cloned DNA sequence.
Molecular cloning is a powerful tool in molecular biology and has numerous applications, including the production of recombinant proteins, gene therapy, functional analysis of genes, and genetic engineering.
Gene expression is the process by which the information encoded in a gene is used to synthesize a functional gene product, such as a protein or RNA molecule. This process involves several steps: transcription, RNA processing, and translation. During transcription, the genetic information in DNA is copied into a complementary RNA molecule, known as messenger RNA (mRNA). The mRNA then undergoes RNA processing, which includes adding a cap and tail to the mRNA and splicing out non-coding regions called introns. The resulting mature mRNA is then translated into a protein on ribosomes in the cytoplasm through the process of translation.
The regulation of gene expression is a complex and highly controlled process that allows cells to respond to changes in their environment, such as growth factors, hormones, and stress signals. This regulation can occur at various stages of gene expression, including transcriptional activation or repression, RNA processing, mRNA stability, and translation. Dysregulation of gene expression has been implicated in many diseases, including cancer, genetic disorders, and neurological conditions.
A cell line is a culture of cells that are grown in a laboratory for use in research. These cells are usually taken from a single cell or group of cells, and they are able to divide and grow continuously in the lab. Cell lines can come from many different sources, including animals, plants, and humans. They are often used in scientific research to study cellular processes, disease mechanisms, and to test new drugs or treatments. Some common types of human cell lines include HeLa cells (which come from a cancer patient named Henrietta Lacks), HEK293 cells (which come from embryonic kidney cells), and HUVEC cells (which come from umbilical vein endothelial cells). It is important to note that cell lines are not the same as primary cells, which are cells that are taken directly from a living organism and have not been grown in the lab.
Green Fluorescent Protein (GFP) is not a medical term per se, but a scientific term used in the field of molecular biology. GFP is a protein that exhibits bright green fluorescence when exposed to light, particularly blue or ultraviolet light. It was originally discovered in the jellyfish Aequorea victoria.
In medical and biological research, scientists often use recombinant DNA technology to introduce the gene for GFP into other organisms, including bacteria, plants, and animals, including humans. This allows them to track the expression and localization of specific genes or proteins of interest in living cells, tissues, or even whole organisms.
The ability to visualize specific cellular structures or processes in real-time has proven invaluable for a wide range of research areas, from studying the development and function of organs and organ systems to understanding the mechanisms of diseases and the effects of therapeutic interventions.
Genetic engineering, also known as genetic modification, is a scientific process where the DNA or genetic material of an organism is manipulated to bring about a change in its characteristics. This is typically done by inserting specific genes into the organism's genome using various molecular biology techniques. These new genes may come from the same species (cisgenesis) or a different species (transgenesis). The goal is to produce a desired trait, such as resistance to pests, improved nutritional content, or increased productivity. It's widely used in research, medicine, and agriculture. However, it's important to note that the use of genetically engineered organisms can raise ethical, environmental, and health concerns.
Arachnid vectors are arthropods belonging to the class Arachnida that are capable of transmitting infectious diseases to humans and other animals. Arachnids include spiders, scorpions, mites, and ticks. Among these, ticks and some mites are the most significant as disease vectors.
Ticks can transmit a variety of bacterial, viral, and protozoan pathogens, causing diseases such as Lyme disease, Rocky Mountain spotted fever, anaplasmosis, ehrlichiosis, babesiosis, tularemia, and several types of encephalitis. They attach to the host's skin and feed on their blood, during which they can transmit pathogens from their saliva.
Mites, particularly chiggers and some species of birds and rodents mites, can also act as vectors for certain diseases, such as scrub typhus and rickettsialpox. Mites are tiny arachnids that live on the skin or in the nests of their hosts and feed on their skin cells, fluids, or blood.
It is important to note that not all arachnids are disease vectors, and only a small percentage of them can transmit infectious diseases. However, those that do pose a significant public health risk and require proper prevention measures, such as using insect repellents, wearing protective clothing, and checking for and promptly removing attached ticks.
Helper viruses, also known as "auxiliary" or "satellite" viruses, are defective viruses that depend on the assistance of a second virus, called a helper virus, to complete their replication cycle. They lack certain genes that are essential for replication, and therefore require the helper virus to provide these functions.
Helper viruses are often found in cases of dual infection, where both the helper virus and the dependent virus infect the same cell. The helper virus provides the necessary enzymes and proteins for the helper virus to replicate, package its genome into new virions, and bud off from the host cell.
One example of a helper virus is the hepatitis B virus (HBV), which can serve as a helper virus for hepatitis D virus (HDV) infection. HDV is a defective RNA virus that requires the HBV surface antigen to form an envelope around its nucleocapsid and be transmitted to other cells. In the absence of HBV, HDV cannot replicate or cause disease.
Understanding the role of helper viruses in viral infections is important for developing effective treatments and vaccines against viral diseases.
"Aedes" is a genus of mosquitoes that are known to transmit various diseases, including Zika virus, dengue fever, chikungunya, and yellow fever. These mosquitoes are typically found in tropical and subtropical regions around the world. They are distinguished by their black and white striped legs and thorax. Aedes aegypti is the most common species associated with disease transmission, although other species such as Aedes albopictus can also transmit diseases. It's important to note that only female mosquitoes bite and feed on blood, while males feed solely on nectar and plant juices.
'Mosquito Control' is not a medical term per se, but it is a public health concept that refers to the systematic reduction or elimination of mosquito populations through various methods to prevent or minimize the transmission of mosquito-borne diseases. This multidisciplinary field involves entomologists, ecologists, engineers, and public health professionals working together to manage mosquito habitats, apply insecticides, and educate communities about personal protection measures. By controlling mosquito populations, we can significantly reduce the risk of contracting vector-borne illnesses such as malaria, dengue fever, yellow fever, Zika virus, and West Nile virus, among others.
Promoter regions in genetics refer to specific DNA sequences located near the transcription start site of a gene. They serve as binding sites for RNA polymerase and various transcription factors that regulate the initiation of gene transcription. These regulatory elements help control the rate of transcription and, therefore, the level of gene expression. Promoter regions can be composed of different types of sequences, such as the TATA box and CAAT box, and their organization and composition can vary between different genes and species.
Adenovirus E1 proteins are the earliest expressed and most critical proteins in the replication cycle of adenoviruses. The "E" stands for "early," indicating that these proteins are produced before the viral DNA begins to replicate. The E1 proteins play a crucial role in regulating the viral life cycle, altering cellular processes to support efficient viral replication, and inhibiting the host's antiviral responses.
The adenovirus E1 proteins are divided into two groups: E1A and E1B.
1. E1A proteins: These proteins are involved in transactivating various viral and cellular promoters, which leads to the expression of early and late viral genes. They also interact with several cellular proteins to alter the host cell cycle, promote cell growth, and inhibit apoptosis (programmed cell death). E1A proteins are essential for efficient viral replication and can transform cells in culture, contributing to adenovirus-induced tumorigenesis in certain animal models.
2. E1B proteins: These proteins have multiple functions during the viral life cycle. E1B 55kDa protein is a potent inhibitor of apoptosis and contributes to efficient viral replication by preventing premature cell death. It also interacts with several cellular proteins, including tumor suppressor p53, to modulate their functions. The E1B 19kDa protein, on the other hand, is a DNA-binding protein that plays a role in viral mRNA processing and export from the nucleus.
Together, adenovirus E1 proteins are essential for successful viral replication and manipulate host cellular processes to create a favorable environment for viral propagation. Understanding their functions has contributed significantly to our knowledge of viral pathogenesis and cancer biology.
Beta-galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. It is found in various organisms, including bacteria, yeast, and mammals. In humans, it plays a role in the breakdown and absorption of certain complex carbohydrates, such as lactose, in the small intestine. Deficiency of this enzyme in humans can lead to a disorder called lactose intolerance. In scientific research, beta-galactosidase is often used as a marker for gene expression and protein localization studies.
'Insect control' is not a term typically used in medical definitions. However, it generally refers to the methods and practices used to manage or reduce the population of insects that can be harmful or disruptive to human health, food supply, or property. This can include various strategies such as chemical pesticides, biological control agents, habitat modification, and other integrated pest management techniques.
In medical terms, 'vector control' is a more relevant concept, which refers to the specific practices used to reduce or prevent the transmission of infectious diseases by insects and other arthropods that act as disease vectors (such as mosquitoes, ticks, and fleas). Vector control measures may include the use of insecticides, larvicides, biological control agents, environmental management, personal protection methods, and other integrated vector management strategies.
Luminescent proteins are a type of protein that emit light through a chemical reaction, rather than by absorbing and re-emitting light like fluorescent proteins. This process is called bioluminescence. The light emitted by luminescent proteins is often used in scientific research as a way to visualize and track biological processes within cells and organisms.
One of the most well-known luminescent proteins is Green Fluorescent Protein (GFP), which was originally isolated from jellyfish. However, GFP is actually a fluorescent protein, not a luminescent one. A true example of a luminescent protein is the enzyme luciferase, which is found in fireflies and other bioluminescent organisms. When luciferase reacts with its substrate, luciferin, it produces light through a process called oxidation.
Luminescent proteins have many applications in research, including as reporters for gene expression, as markers for protein-protein interactions, and as tools for studying the dynamics of cellular processes. They are also used in medical imaging and diagnostics, as well as in the development of new therapies.
'Culicidae' is the biological family that includes all species of mosquitoes. It consists of three subfamilies: Anophelinae, Culicinae, and Toxorhynchitinae. Mosquitoes are small, midge-like flies that are known for their ability to transmit various diseases to humans and other animals, such as malaria, yellow fever, dengue fever, and Zika virus. The medical importance of Culicidae comes from the fact that only female mosquitoes require blood meals to lay eggs, and during this process, they can transmit pathogens between hosts.
A "reporter gene" is a type of gene that is linked to a gene of interest in order to make the expression or activity of that gene detectable. The reporter gene encodes for a protein that can be easily measured and serves as an indicator of the presence and activity of the gene of interest. Commonly used reporter genes include those that encode for fluorescent proteins, enzymes that catalyze colorimetric reactions, or proteins that bind to specific molecules.
In the context of genetics and genomics research, a reporter gene is often used in studies involving gene expression, regulation, and function. By introducing the reporter gene into an organism or cell, researchers can monitor the activity of the gene of interest in real-time or after various experimental treatments. The information obtained from these studies can help elucidate the role of specific genes in biological processes and diseases, providing valuable insights for basic research and therapeutic development.
'Anopheles gambiae' is a species of mosquito that is a major vector for the transmission of malaria. The female Anopheles gambiae mosquito bites primarily during the nighttime hours and preferentially feeds on human blood, which allows it to transmit the Plasmodium parasite that causes malaria. This species is widely distributed throughout much of Africa and is responsible for transmitting a significant proportion of the world's malaria cases.
The Anopheles gambiae complex actually consists of several closely related species or forms, which can be difficult to distinguish based on morphological characteristics alone. However, advances in molecular techniques have allowed for more accurate identification and differentiation of these species. Understanding the biology and behavior of Anopheles gambiae is crucial for developing effective strategies to control malaria transmission.
'Culex' is a genus of mosquitoes that includes many species that are vectors for various diseases, such as West Nile virus, filariasis, and avian malaria. They are often referred to as "house mosquitoes" because they are commonly found in urban environments. These mosquitoes typically lay their eggs in standing water and have a cosmopolitan distribution, being found on all continents except Antarctica. The life cycle of Culex mosquitoes includes four stages: egg, larva, pupa, and adult. Both male and female adults feed on nectar, but only females require blood meals to lay eggs.
Genetic recombination is the process by which genetic material is exchanged between two similar or identical molecules of DNA during meiosis, resulting in new combinations of genes on each chromosome. This exchange occurs during crossover, where segments of DNA are swapped between non-sister homologous chromatids, creating genetic diversity among the offspring. It is a crucial mechanism for generating genetic variability and facilitating evolutionary change within populations. Additionally, recombination also plays an essential role in DNA repair processes through mechanisms such as homologous recombinational repair (HRR) and non-homologous end joining (NHEJ).
'Escherichia coli' (E. coli) is a type of gram-negative, facultatively anaerobic, rod-shaped bacterium that commonly inhabits the intestinal tract of humans and warm-blooded animals. It is a member of the family Enterobacteriaceae and one of the most well-studied prokaryotic model organisms in molecular biology.
While most E. coli strains are harmless and even beneficial to their hosts, some serotypes can cause various forms of gastrointestinal and extraintestinal illnesses in humans and animals. These pathogenic strains possess virulence factors that enable them to colonize and damage host tissues, leading to diseases such as diarrhea, urinary tract infections, pneumonia, and sepsis.
E. coli is a versatile organism with remarkable genetic diversity, which allows it to adapt to various environmental niches. It can be found in water, soil, food, and various man-made environments, making it an essential indicator of fecal contamination and a common cause of foodborne illnesses. The study of E. coli has contributed significantly to our understanding of fundamental biological processes, including DNA replication, gene regulation, and protein synthesis.
Adenoviruses, Human: A group of viruses that commonly cause respiratory illnesses, such as bronchitis, pneumonia, and croup, in humans. They can also cause conjunctivitis (pink eye), cystitis (bladder infection), and gastroenteritis (stomach and intestinal infection).
Human adenoviruses are non-enveloped, double-stranded DNA viruses that belong to the family Adenoviridae. There are more than 50 different types of human adenoviruses, which can be classified into seven species (A-G). Different types of adenoviruses tend to cause specific illnesses, such as respiratory or gastrointestinal infections.
Human adenoviruses are highly contagious and can spread through close personal contact, respiratory droplets, or contaminated surfaces. They can also be transmitted through contaminated water sources. Some people may become carriers of the virus and experience no symptoms but still spread the virus to others.
Most human adenovirus infections are mild and resolve on their own within a few days to a week. However, some types of adenoviruses can cause severe illness, particularly in people with weakened immune systems, such as infants, young children, older adults, and individuals with HIV/AIDS or organ transplants.
There are no specific antiviral treatments for human adenovirus infections, but supportive care, such as hydration, rest, and fever reduction, can help manage symptoms. Preventive measures include practicing good hygiene, such as washing hands frequently, avoiding close contact with sick individuals, and not sharing personal items like towels or utensils.
Insecticides are substances or mixtures of substances intended for preventing, destroying, or mitigating any pest, including insects, arachnids, or other related pests. They can be chemical or biological agents that disrupt the growth, development, or behavior of these organisms, leading to their death or incapacitation. Insecticides are widely used in agriculture, public health, and residential settings for pest control. However, they must be used with caution due to potential risks to non-target organisms and the environment.
Recombinant fusion proteins are artificially created biomolecules that combine the functional domains or properties of two or more different proteins into a single protein entity. They are generated through recombinant DNA technology, where the genes encoding the desired protein domains are linked together and expressed as a single, chimeric gene in a host organism, such as bacteria, yeast, or mammalian cells.
The resulting fusion protein retains the functional properties of its individual constituent proteins, allowing for novel applications in research, diagnostics, and therapeutics. For instance, recombinant fusion proteins can be designed to enhance protein stability, solubility, or immunogenicity, making them valuable tools for studying protein-protein interactions, developing targeted therapies, or generating vaccines against infectious diseases or cancer.
Examples of recombinant fusion proteins include:
1. Etaglunatide (ABT-523): A soluble Fc fusion protein that combines the heavy chain fragment crystallizable region (Fc) of an immunoglobulin with the extracellular domain of the human interleukin-6 receptor (IL-6R). This fusion protein functions as a decoy receptor, neutralizing IL-6 and its downstream signaling pathways in rheumatoid arthritis.
2. Etanercept (Enbrel): A soluble TNF receptor p75 Fc fusion protein that binds to tumor necrosis factor-alpha (TNF-α) and inhibits its proinflammatory activity, making it a valuable therapeutic option for treating autoimmune diseases like rheumatoid arthritis, ankylosing spondylitis, and psoriasis.
3. Abatacept (Orencia): A fusion protein consisting of the extracellular domain of cytotoxic T-lymphocyte antigen 4 (CTLA-4) linked to the Fc region of an immunoglobulin, which downregulates T-cell activation and proliferation in autoimmune diseases like rheumatoid arthritis.
4. Belimumab (Benlysta): A monoclonal antibody that targets B-lymphocyte stimulator (BLyS) protein, preventing its interaction with the B-cell surface receptor and inhibiting B-cell activation in systemic lupus erythematosus (SLE).
5. Romiplostim (Nplate): A fusion protein consisting of a thrombopoietin receptor agonist peptide linked to an immunoglobulin Fc region, which stimulates platelet production in patients with chronic immune thrombocytopenia (ITP).
6. Darbepoetin alfa (Aranesp): A hyperglycosylated erythropoiesis-stimulating protein that functions as a longer-acting form of recombinant human erythropoietin, used to treat anemia in patients with chronic kidney disease or cancer.
7. Palivizumab (Synagis): A monoclonal antibody directed against the F protein of respiratory syncytial virus (RSV), which prevents RSV infection and is administered prophylactically to high-risk infants during the RSV season.
8. Ranibizumab (Lucentis): A recombinant humanized monoclonal antibody fragment that binds and inhibits vascular endothelial growth factor A (VEGF-A), used in the treatment of age-related macular degeneration, diabetic retinopathy, and other ocular disorders.
9. Cetuximab (Erbitux): A chimeric monoclonal antibody that binds to epidermal growth factor receptor (EGFR), used in the treatment of colorectal cancer and head and neck squamous cell carcinoma.
10. Adalimumab (Humira): A fully humanized monoclonal antibody that targets tumor necrosis factor-alpha (TNF-α), used in the treatment of various inflammatory diseases, including rheumatoid arthritis, psoriasis, and Crohn's disease.
11. Bevacizumab (Avastin): A recombinant humanized monoclonal antibody that binds to VEGF-A, used in the treatment of various cancers, including colorectal, lung, breast, and kidney cancer.
12. Trastuzumab (Herceptin): A humanized monoclonal antibody that targets HER2/neu receptor, used in the treatment of breast cancer.
13. Rituximab (Rituxan): A chimeric monoclonal antibody that binds to CD20 antigen on B cells, used in the treatment of non-Hodgkin's lymphoma and rheumatoid arthritis.
14. Palivizumab (Synagis): A humanized monoclonal antibody that binds to the F protein of respiratory syncytial virus, used in the prevention of respiratory syncytial virus infection in high-risk infants.
15. Infliximab (Remicade): A chimeric monoclonal antibody that targets TNF-α, used in the treatment of various inflammatory diseases, including Crohn's disease, ulcerative colitis, rheumatoid arthritis, and ankylosing spondylitis.
16. Natalizumab (Tysabri): A humanized monoclonal antibody that binds to α4β1 integrin, used in the treatment of multiple sclerosis and Crohn's disease.
17. Adalimumab (Humira): A fully human monoclonal antibody that targets TNF-α, used in the treatment of various inflammatory diseases, including rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, and ulcerative colitis.
18. Golimumab (Simponi): A fully human monoclonal antibody that targets TNF-α, used in the treatment of rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, and ulcerative colitis.
19. Certolizumab pegol (Cimzia): A PEGylated Fab' fragment of a humanized monoclonal antibody that targets TNF-α, used in the treatment of rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, and Crohn's disease.
20. Ustekinumab (Stelara): A fully human monoclonal antibody that targets IL-12 and IL-23, used in the treatment of psoriasis, psoriatic arthritis, and Crohn's disease.
21. Secukinumab (Cosentyx): A fully human monoclonal antibody that targets IL-17A, used in the treatment of psoriasis, psoriatic arthritis, and ankylosing spondylitis.
22. Ixekizumab (Taltz): A fully human monoclonal antibody that targets IL-17A, used in the treatment of psoriasis and psoriatic arthritis.
23. Brodalumab (Siliq): A fully human monoclonal antibody that targets IL-17 receptor A, used in the treatment of psoriasis.
24. Sarilumab (Kevzara): A fully human monoclonal antibody that targets the IL-6 receptor, used in the treatment of rheumatoid arthritis.
25. Tocilizumab (Actemra): A humanized monoclonal antibody that targets the IL-6 receptor, used in the treatment of rheumatoid arthritis, systemic juvenile idiopathic arthritis, polyarticular juvenile idiopathic arthritis, giant cell arteritis, and chimeric antigen receptor T-cell-induced cytokine release syndrome.
26. Siltuximab (Sylvant): A chimeric monoclonal antibody that targets IL-6, used in the treatment of multicentric Castleman disease.
27. Satralizumab (Enspryng): A humanized monoclonal antibody that targets IL-6 receptor alpha, used in the treatment of neuromyelitis optica spectrum disorder.
28. Sirukumab (Plivensia): A human monoclonal antibody that targets IL-6, used in the treatment
Recombinant DNA is a term used in molecular biology to describe DNA that has been created by combining genetic material from more than one source. This is typically done through the use of laboratory techniques such as molecular cloning, in which fragments of DNA are inserted into vectors (such as plasmids or viruses) and then introduced into a host organism where they can replicate and produce many copies of the recombinant DNA molecule.
Recombinant DNA technology has numerous applications in research, medicine, and industry, including the production of recombinant proteins for use as therapeutics, the creation of genetically modified organisms (GMOs) for agricultural or industrial purposes, and the development of new tools for genetic analysis and manipulation.
It's important to note that while recombinant DNA technology has many potential benefits, it also raises ethical and safety concerns, and its use is subject to regulation and oversight in many countries.
Malaria is not a medical definition itself, but it is a disease caused by parasites that are transmitted to people through the bites of infected female Anopheles mosquitoes. Here's a simple definition:
Malaria: A mosquito-borne infectious disease caused by Plasmodium parasites, characterized by cycles of fever, chills, and anemia. It can be fatal if not promptly diagnosed and treated. The five Plasmodium species known to cause malaria in humans are P. falciparum, P. vivax, P. ovale, P. malariae, and P. knowlesi.
An amino acid sequence is the specific order of amino acids in a protein or peptide molecule, formed by the linking of the amino group (-NH2) of one amino acid to the carboxyl group (-COOH) of another amino acid through a peptide bond. The sequence is determined by the genetic code and is unique to each type of protein or peptide. It plays a crucial role in determining the three-dimensional structure and function of proteins.
Virus integration, in the context of molecular biology and virology, refers to the insertion of viral genetic material into the host cell's genome. This process is most commonly associated with retroviruses, such as HIV (Human Immunodeficiency Virus), which have an enzyme called reverse transcriptase that converts their RNA genome into DNA. This DNA can then integrate into the host's chromosomal DNA, becoming a permanent part of the host's genetic material.
This integration is a crucial step in the retroviral life cycle, allowing the virus to persist within the host cell and evade detection by the immune system. It also means that the viral genome can be passed on to daughter cells when the host cell divides.
However, it's important to note that not all viruses integrate their genetic material into the host's genome. Some viruses, like influenza, exist as separate entities within the host cell and do not become part of the host's DNA.
Gene targeting is a research technique in molecular biology used to precisely modify specific genes within the genome of an organism. This technique allows scientists to study gene function by creating targeted genetic changes, such as insertions, deletions, or mutations, in a specific gene of interest. The process typically involves the use of engineered nucleases, such as CRISPR-Cas9 or TALENs, to introduce double-stranded breaks at desired locations within the genome. These breaks are then repaired by the cell's own DNA repair machinery, often leading to the incorporation of designed changes in the targeted gene. Gene targeting is a powerful tool for understanding gene function and has wide-ranging applications in basic research, agriculture, and therapeutic development.
Recombinant proteins are artificially created proteins produced through the use of recombinant DNA technology. This process involves combining DNA molecules from different sources to create a new set of genes that encode for a specific protein. The resulting recombinant protein can then be expressed, purified, and used for various applications in research, medicine, and industry.
Recombinant proteins are widely used in biomedical research to study protein function, structure, and interactions. They are also used in the development of diagnostic tests, vaccines, and therapeutic drugs. For example, recombinant insulin is a common treatment for diabetes, while recombinant human growth hormone is used to treat growth disorders.
The production of recombinant proteins typically involves the use of host cells, such as bacteria, yeast, or mammalian cells, which are engineered to express the desired protein. The host cells are transformed with a plasmid vector containing the gene of interest, along with regulatory elements that control its expression. Once the host cells are cultured and the protein is expressed, it can be purified using various chromatography techniques.
Overall, recombinant proteins have revolutionized many areas of biology and medicine, enabling researchers to study and manipulate proteins in ways that were previously impossible.
The lac operon is a genetic regulatory system found in the bacteria Escherichia coli that controls the expression of genes responsible for the metabolism of lactose as a source of energy. It consists of three structural genes (lacZ, lacY, and lacA) that code for enzymes involved in lactose metabolism, as well as two regulatory elements: the lac promoter and the lac operator.
The lac repressor protein, produced by the lacI gene, binds to the lac operator sequence when lactose is not present, preventing RNA polymerase from transcribing the structural genes. When lactose is available, it is converted into allolactose, which acts as an inducer and binds to the lac repressor protein, causing a conformational change that prevents it from binding to the operator sequence. This allows RNA polymerase to bind to the promoter and transcribe the structural genes, leading to the production of enzymes necessary for lactose metabolism.
In summary, the lac operon is a genetic regulatory system in E. coli that controls the expression of genes involved in lactose metabolism based on the availability of lactose as a substrate.
Genetic transformation is the process by which an organism's genetic material is altered or modified, typically through the introduction of foreign DNA. This can be achieved through various techniques such as:
* Gene transfer using vectors like plasmids, phages, or artificial chromosomes
* Direct uptake of naked DNA using methods like electroporation or chemically-mediated transfection
* Use of genome editing tools like CRISPR-Cas9 to introduce precise changes into the organism's genome.
The introduced DNA may come from another individual of the same species (cisgenic), from a different species (transgenic), or even be synthetically designed. The goal of genetic transformation is often to introduce new traits, functions, or characteristics that do not exist naturally in the organism, or to correct genetic defects.
This technique has broad applications in various fields, including molecular biology, biotechnology, and medical research, where it can be used to study gene function, develop genetically modified organisms (GMOs), create cell lines for drug screening, and even potentially treat genetic diseases through gene therapy.
Virus replication is the process by which a virus produces copies or reproduces itself inside a host cell. This involves several steps:
1. Attachment: The virus attaches to a specific receptor on the surface of the host cell.
2. Penetration: The viral genetic material enters the host cell, either by invagination of the cell membrane or endocytosis.
3. Uncoating: The viral genetic material is released from its protective coat (capsid) inside the host cell.
4. Replication: The viral genetic material uses the host cell's machinery to produce new viral components, such as proteins and nucleic acids.
5. Assembly: The newly synthesized viral components are assembled into new virus particles.
6. Release: The newly formed viruses are released from the host cell, often through lysis (breaking) of the cell membrane or by budding off the cell membrane.
The specific mechanisms and details of virus replication can vary depending on the type of virus. Some viruses, such as DNA viruses, use the host cell's DNA polymerase to replicate their genetic material, while others, such as RNA viruses, use their own RNA-dependent RNA polymerase or reverse transcriptase enzymes. Understanding the process of virus replication is important for developing antiviral therapies and vaccines.
Polymerase Chain Reaction (PCR) is a laboratory technique used to amplify specific regions of DNA. It enables the production of thousands to millions of copies of a particular DNA sequence in a rapid and efficient manner, making it an essential tool in various fields such as molecular biology, medical diagnostics, forensic science, and research.
The PCR process involves repeated cycles of heating and cooling to separate the DNA strands, allow primers (short sequences of single-stranded DNA) to attach to the target regions, and extend these primers using an enzyme called Taq polymerase, resulting in the exponential amplification of the desired DNA segment.
In a medical context, PCR is often used for detecting and quantifying specific pathogens (viruses, bacteria, fungi, or parasites) in clinical samples, identifying genetic mutations or polymorphisms associated with diseases, monitoring disease progression, and evaluating treatment effectiveness.
Psychodidae is a family of small, delicate flies known as psychodids or moth flies. The term "psychodidae" itself is the taxonomic name for this group of insects, and it does not have a specific medical definition. However, some species within this family are known to be vectors of various diseases, such as Leishmaniasis, which is transmitted through the bites of infected sandflies (a type of psychodid).
Therefore, in a broader medical context, "psychodidae" may refer to the group of flies that includes potential disease-carrying species. It's important to note that not all psychodids are vectors of diseases, and many species are harmless to humans.
"Phlebotomus" is a genus of sandflies, which are small flies that are known to transmit various diseases such as leishmaniasis. These flies are typically found in warm and humid regions around the world, particularly in the Mediterranean, Middle East, Africa, and Asia. The females of this genus feed on the blood of mammals, including humans, for egg production. It is important to note that not all species of Phlebotomus are vectors of disease, but those that are can cause significant public health concerns in affected areas.
Triatoma is a genus of insects in the family Reduviidae, also known as "kissing bugs" or "conenose bugs." These insects are called "kissing bugs" because they often bite humans around the mouth and face. They are found primarily in the Americas, ranging from the southern United States to Argentina.
Triatoma species are of medical importance because they can transmit a parasitic infection called Chagas disease (American trypanosomiasis) to humans through their feces. The parasite that causes Chagas disease, Trypanosoma cruzi, is found in the bug's feces and can enter the human body through mucous membranes or breaks in the skin.
Chagas disease can cause serious health problems, including heart damage and digestive system complications, if left untreated. Therefore, it is important to take precautions to prevent Triatoma bites and seek medical attention promptly if bitten by one of these insects.
"Cells, cultured" is a medical term that refers to cells that have been removed from an organism and grown in controlled laboratory conditions outside of the body. This process is called cell culture and it allows scientists to study cells in a more controlled and accessible environment than they would have inside the body. Cultured cells can be derived from a variety of sources, including tissues, organs, or fluids from humans, animals, or cell lines that have been previously established in the laboratory.
Cell culture involves several steps, including isolation of the cells from the tissue, purification and characterization of the cells, and maintenance of the cells in appropriate growth conditions. The cells are typically grown in specialized media that contain nutrients, growth factors, and other components necessary for their survival and proliferation. Cultured cells can be used for a variety of purposes, including basic research, drug development and testing, and production of biological products such as vaccines and gene therapies.
It is important to note that cultured cells may behave differently than they do in the body, and results obtained from cell culture studies may not always translate directly to human physiology or disease. Therefore, it is essential to validate findings from cell culture experiments using additional models and ultimately in clinical trials involving human subjects.
BALB/c is an inbred strain of laboratory mouse that is widely used in biomedical research. The strain was developed at the Institute of Cancer Research in London by Henry Baldwin and his colleagues in the 1920s, and it has since become one of the most commonly used inbred strains in the world.
BALB/c mice are characterized by their black coat color, which is determined by a recessive allele at the tyrosinase locus. They are also known for their docile and friendly temperament, making them easy to handle and work with in the laboratory.
One of the key features of BALB/c mice that makes them useful for research is their susceptibility to certain types of tumors and immune responses. For example, they are highly susceptible to developing mammary tumors, which can be induced by chemical carcinogens or viral infection. They also have a strong Th2-biased immune response, which makes them useful models for studying allergic diseases and asthma.
BALB/c mice are also commonly used in studies of genetics, neuroscience, behavior, and infectious diseases. Because they are an inbred strain, they have a uniform genetic background, which makes it easier to control for genetic factors in experiments. Additionally, because they have been bred in the laboratory for many generations, they are highly standardized and reproducible, making them ideal subjects for scientific research.
Ceratopogonidae is a family of small flies, also known as biting midges or no-see-ums. They are characterized by their slender segmented bodies, feathery antennae, and wings with extensive venation. Some species in this family are known to be vectors of various diseases, such as human and animal forms of filariasis, blue tongue virus in sheep, and several viral diseases in horses. The larvae of these flies are aquatic or semi-aquatic and can be found in a variety of habitats including wet soil, decaying vegetation, and freshwater bodies.
'Tumor cells, cultured' refers to the process of removing cancerous cells from a tumor and growing them in controlled laboratory conditions. This is typically done by isolating the tumor cells from a patient's tissue sample, then placing them in a nutrient-rich environment that promotes their growth and multiplication.
The resulting cultured tumor cells can be used for various research purposes, including the study of cancer biology, drug development, and toxicity testing. They provide a valuable tool for researchers to better understand the behavior and characteristics of cancer cells outside of the human body, which can lead to the development of more effective cancer treatments.
It is important to note that cultured tumor cells may not always behave exactly the same way as they do in the human body, so findings from cell culture studies must be validated through further research, such as animal models or clinical trials.
Synthetic vaccines are artificially produced, designed to stimulate an immune response and provide protection against specific diseases. Unlike traditional vaccines that are derived from weakened or killed pathogens, synthetic vaccines are created using synthetic components, such as synthesized viral proteins, DNA, or RNA. These components mimic the disease-causing agent and trigger an immune response without causing the actual disease. The use of synthetic vaccines offers advantages in terms of safety, consistency, and scalability in production, making them valuable tools for preventing infectious diseases.
DNA primers are short single-stranded DNA molecules that serve as a starting point for DNA synthesis. They are typically used in laboratory techniques such as the polymerase chain reaction (PCR) and DNA sequencing. The primer binds to a complementary sequence on the DNA template through base pairing, providing a free 3'-hydroxyl group for the DNA polymerase enzyme to add nucleotides and synthesize a new strand of DNA. This allows for specific and targeted amplification or analysis of a particular region of interest within a larger DNA molecule.
Insecticide resistance is a genetic selection process in insect populations that allows them to survive and reproduce despite exposure to insecticides. It's the result of changes in the genetic makeup of insects, which can be caused by natural selection when insecticides are used repeatedly. Over time, this leads to the prevalence of genes that provide resistance to the insecticide, making the pest control methods less effective. Insecticide resistance is a significant challenge in public health and agriculture, as it can reduce the efficacy of interventions aimed at controlling disease-carrying insects or protecting crops from pests.
Defective viruses are viruses that have lost the ability to complete a full replication cycle and produce progeny virions independently. These viruses require the assistance of a helper virus, which provides the necessary functions for replication. Defective viruses can arise due to mutations, deletions, or other genetic changes that result in the loss of essential genes. They are often non-infectious and cannot cause disease on their own, but they may interfere with the replication of the helper virus and modulate the course of infection. Defective viruses can be found in various types of viruses, including retroviruses, bacteriophages, and DNA viruses.
C57BL/6 (C57 Black 6) is an inbred strain of laboratory mouse that is widely used in biomedical research. The term "inbred" refers to a strain of animals where matings have been carried out between siblings or other closely related individuals for many generations, resulting in a population that is highly homozygous at most genetic loci.
The C57BL/6 strain was established in 1920 by crossing a female mouse from the dilute brown (DBA) strain with a male mouse from the black strain. The resulting offspring were then interbred for many generations to create the inbred C57BL/6 strain.
C57BL/6 mice are known for their robust health, longevity, and ease of handling, making them a popular choice for researchers. They have been used in a wide range of biomedical research areas, including studies of cancer, immunology, neuroscience, cardiovascular disease, and metabolism.
One of the most notable features of the C57BL/6 strain is its sensitivity to certain genetic modifications, such as the introduction of mutations that lead to obesity or impaired glucose tolerance. This has made it a valuable tool for studying the genetic basis of complex diseases and traits.
Overall, the C57BL/6 inbred mouse strain is an important model organism in biomedical research, providing a valuable resource for understanding the genetic and molecular mechanisms underlying human health and disease.
Kanamycin Kinase is not a widely recognized medical term, but it is a concept from the field of microbiology. It refers to an enzyme produced by certain bacteria that catalyzes the phosphorylation of kanamycin, an aminoglycoside antibiotic. The phosphorylation of kanamycin inactivates its antibacterial activity, making it less effective against those bacteria that produce this kinase. This is one mechanism by which some bacteria develop resistance to antibiotics.
'Gene expression regulation' refers to the processes that control whether, when, and where a particular gene is expressed, meaning the production of a specific protein or functional RNA encoded by that gene. This complex mechanism can be influenced by various factors such as transcription factors, chromatin remodeling, DNA methylation, non-coding RNAs, and post-transcriptional modifications, among others. Proper regulation of gene expression is crucial for normal cellular function, development, and maintaining homeostasis in living organisms. Dysregulation of gene expression can lead to various diseases, including cancer and genetic disorders.
The Moloney murine leukemia virus (Mo-MLV) is a type of retrovirus, specifically a gammaretrovirus, that is commonly found in mice. It was first discovered and isolated by John Moloney in 1960. Mo-MLV is known to cause various types of cancerous conditions, particularly leukemia, in susceptible mouse strains.
Mo-MLV has a single-stranded RNA genome that is reverse transcribed into double-stranded DNA upon infection of the host cell. This viral DNA then integrates into the host's genome and utilizes the host's cellular machinery to produce new virus particles. The Mo-MLV genome encodes for several viral proteins, including gag (group-specific antigen), pol (polymerase), and env (envelope) proteins, which are essential for the replication cycle of the virus.
Mo-MLV is widely used in laboratory research as a model retrovirus to study various aspects of viral replication, gene therapy, and oncogenesis. It has also been engineered as a vector for gene delivery applications due to its ability to efficiently integrate into the host genome and deliver large DNA sequences. However, it is important to note that Mo-MLV and other retroviruses have the potential to cause insertional mutagenesis, which can lead to unintended genetic alterations and adverse effects in some cases.
Deoxyribonucleic acid (DNA) is the genetic material present in the cells of organisms where it is responsible for the storage and transmission of hereditary information. DNA is a long molecule that consists of two strands coiled together to form a double helix. Each strand is made up of a series of four nucleotide bases - adenine (A), guanine (G), cytosine (C), and thymine (T) - that are linked together by phosphate and sugar groups. The sequence of these bases along the length of the molecule encodes genetic information, with A always pairing with T and C always pairing with G. This base-pairing allows for the replication and transcription of DNA, which are essential processes in the functioning and reproduction of all living organisms.
Genetic transcription is the process by which the information in a strand of DNA is used to create a complementary RNA molecule. This process is the first step in gene expression, where the genetic code in DNA is converted into a form that can be used to produce proteins or functional RNAs.
During transcription, an enzyme called RNA polymerase binds to the DNA template strand and reads the sequence of nucleotide bases. As it moves along the template, it adds complementary RNA nucleotides to the growing RNA chain, creating a single-stranded RNA molecule that is complementary to the DNA template strand. Once transcription is complete, the RNA molecule may undergo further processing before it can be translated into protein or perform its functional role in the cell.
Transcription can be either "constitutive" or "regulated." Constitutive transcription occurs at a relatively constant rate and produces essential proteins that are required for basic cellular functions. Regulated transcription, on the other hand, is subject to control by various intracellular and extracellular signals, allowing cells to respond to changing environmental conditions or developmental cues.
Viral DNA refers to the genetic material present in viruses that consist of DNA as their core component. Deoxyribonucleic acid (DNA) is one of the two types of nucleic acids that are responsible for storing and transmitting genetic information in living organisms. Viruses are infectious agents much smaller than bacteria that can only replicate inside the cells of other organisms, called hosts.
Viral DNA can be double-stranded (dsDNA) or single-stranded (ssDNA), depending on the type of virus. Double-stranded DNA viruses have a genome made up of two complementary strands of DNA, while single-stranded DNA viruses contain only one strand of DNA.
Examples of dsDNA viruses include Adenoviruses, Herpesviruses, and Poxviruses, while ssDNA viruses include Parvoviruses and Circoviruses. Viral DNA plays a crucial role in the replication cycle of the virus, encoding for various proteins necessary for its multiplication and survival within the host cell.
Capsid proteins are the structural proteins that make up the capsid, which is the protective shell of a virus. The capsid encloses the viral genome and helps to protect it from degradation and detection by the host's immune system. Capsid proteins are typically arranged in a symmetrical pattern and can self-assemble into the capsid structure when exposed to the viral genome.
The specific arrangement and composition of capsid proteins vary between different types of viruses, and they play important roles in the virus's life cycle, including recognition and binding to host cells, entry into the cell, and release of the viral genome into the host cytoplasm. Capsid proteins can also serve as targets for antiviral therapies and vaccines.
Pyrethrins are a group of naturally occurring organic compounds extracted from the flowers of Chrysanthemum cinerariaefolium and Chrysanthemum coccineum. They have been used for centuries as insecticides due to their ability to disrupt the nervous system of insects, leading to paralysis and death. Pyrethrins are composed of six esters, pyrethrin I and II, cinerin I and II, and jasmolin I and II, which have different insecticidal properties but share a similar mode of action. They are commonly used in household insect sprays, pet shampoos, and agricultural applications to control a wide range of pests. However, pyrethrins can be toxic to fish and some beneficial insects, so they must be used with caution.
A mutation is a permanent change in the DNA sequence of an organism's genome. Mutations can occur spontaneously or be caused by environmental factors such as exposure to radiation, chemicals, or viruses. They may have various effects on the organism, ranging from benign to harmful, depending on where they occur and whether they alter the function of essential proteins. In some cases, mutations can increase an individual's susceptibility to certain diseases or disorders, while in others, they may confer a survival advantage. Mutations are the driving force behind evolution, as they introduce new genetic variability into populations, which can then be acted upon by natural selection.
Restriction mapping is a technique used in molecular biology to identify the location and arrangement of specific restriction endonuclease recognition sites within a DNA molecule. Restriction endonucleases are enzymes that cut double-stranded DNA at specific sequences, producing fragments of various lengths. By digesting the DNA with different combinations of these enzymes and analyzing the resulting fragment sizes through techniques such as agarose gel electrophoresis, researchers can generate a restriction map - a visual representation of the locations and distances between recognition sites on the DNA molecule. This information is crucial for various applications, including cloning, genome analysis, and genetic engineering.
A bacterial gene is a segment of DNA (or RNA in some viruses) that contains the genetic information necessary for the synthesis of a functional bacterial protein or RNA molecule. These genes are responsible for encoding various characteristics and functions of bacteria such as metabolism, reproduction, and resistance to antibiotics. They can be transmitted between bacteria through horizontal gene transfer mechanisms like conjugation, transformation, and transduction. Bacterial genes are often organized into operons, which are clusters of genes that are transcribed together as a single mRNA molecule.
It's important to note that the term "bacterial gene" is used to describe genetic elements found in bacteria, but not all genetic elements in bacteria are considered genes. For example, some DNA sequences may not encode functional products and are therefore not considered genes. Additionally, some bacterial genes may be plasmid-borne or phage-borne, rather than being located on the bacterial chromosome.
I'm sorry for any confusion, but "Hemiptera" is not a medical term. It is a taxonomic order in the classification of living things, also known as "true bugs." This group includes species such as cicadas, aphids, and bedbugs. If you have a medical term in mind, please provide it so I can give you an accurate definition.
Messenger RNA (mRNA) is a type of RNA (ribonucleic acid) that carries genetic information copied from DNA in the form of a series of three-base code "words," each of which specifies a particular amino acid. This information is used by the cell's machinery to construct proteins, a process known as translation. After being transcribed from DNA, mRNA travels out of the nucleus to the ribosomes in the cytoplasm where protein synthesis occurs. Once the protein has been synthesized, the mRNA may be degraded and recycled. Post-transcriptional modifications can also occur to mRNA, such as alternative splicing and addition of a 5' cap and a poly(A) tail, which can affect its stability, localization, and translation efficiency.
The Coxsackie and Adenovirus Receptor (CAR) is a transmembrane protein that serves as a receptor for several viruses, including Coxsackieviruses and certain types of Adenoviruses. The "Coxsackie and Adenovirus Receptor-Like Membrane Protein" likely refers to a membrane protein that shares structural or functional similarities with the CAR protein.
The CAR protein is a member of the immunoglobulin superfamily and is widely expressed in various tissues, including the heart, lungs, and nervous system. It plays important roles in cell adhesion, tissue development, and repair, as well as serving as an entry point for certain viruses to infect cells.
The CAR-like membrane protein may have similar functions or structures to the CAR protein, but its specific identity and role are not clearly defined in the medical literature. It is possible that it could be a target for viral infection or play a role in cellular processes, but further research is needed to confirm these possibilities.
Simian adenoviruses are a group of viruses that primarily infect non-human primates, such as monkeys and apes. They belong to the family Adenoviridae and are closely related to human adenoviruses. Like human adenoviruses, simian adenoviruses can cause a wide range of respiratory, gastrointestinal, and ocular diseases in their hosts.
There are several different species of simian adenoviruses, including species A to G, and each species contains multiple serotypes. Some simian adenoviruses have been associated with severe disease outbreaks in captive primates, while others appear to cause only mild or asymptomatic infections.
Simian adenoviruses are not known to commonly infect humans, but there have been a few reported cases of human infection, usually in individuals who have close contact with non-human primates. In recent years, simian adenoviruses have gained attention as potential vectors for gene therapy and vaccine development. Researchers have engineered simian adenovirus vectors to deliver therapeutic genes or vaccines against various diseases, including HIV, tuberculosis, and COVID-19. However, the use of these vectors in humans is still being studied and requires careful evaluation to ensure safety and efficacy.
HeLa cells are a type of immortalized cell line used in scientific research. They are derived from a cancer that developed in the cervical tissue of Henrietta Lacks, an African-American woman, in 1951. After her death, cells taken from her tumor were found to be capable of continuous division and growth in a laboratory setting, making them an invaluable resource for medical research.
HeLa cells have been used in a wide range of scientific studies, including research on cancer, viruses, genetics, and drug development. They were the first human cell line to be successfully cloned and are able to grow rapidly in culture, doubling their population every 20-24 hours. This has made them an essential tool for many areas of biomedical research.
It is important to note that while HeLa cells have been instrumental in numerous scientific breakthroughs, the story of their origin raises ethical questions about informed consent and the use of human tissue in research.
I'm sorry for any confusion, but "population density" is actually a term used in population geography and epidemiology, rather than medical terminology. It refers to the number of people living in a specific area or region, usually measured as the number of people per square mile or square kilometer.
However, understanding population density can be important in public health and medicine because it can influence various factors related to health outcomes and healthcare delivery, such as:
1. Disease transmission rates: Higher population densities can facilitate the spread of infectious diseases, particularly those that are transmitted through close contact between individuals.
2. Access to healthcare services: Areas with lower population density might have fewer healthcare resources and providers available, making it more challenging for residents to access necessary medical care.
3. Health disparities: Population density can contribute to health inequities, as urban areas often have better access to healthcare, education, and economic opportunities than rural areas, leading to differences in health outcomes between these populations.
4. Environmental factors: Higher population densities might lead to increased pollution, noise, and other environmental hazards that can negatively impact health.
Therefore, while "population density" is not a medical definition per se, it remains an essential concept for understanding various public health and healthcare issues.
Artificial Intelligence (AI) in the medical context refers to the simulation of human intelligence processes by machines, particularly computer systems. These processes include learning (the acquisition of information and rules for using the information), reasoning (using the rules to reach approximate or definite conclusions), and self-correction.
In healthcare, AI is increasingly being used to analyze large amounts of data, identify patterns, make decisions, and perform tasks that would normally require human intelligence. This can include tasks such as diagnosing diseases, recommending treatments, personalizing patient care, and improving clinical workflows.
Examples of AI in medicine include machine learning algorithms that analyze medical images to detect signs of disease, natural language processing tools that extract relevant information from electronic health records, and robot-assisted surgery systems that enable more precise and minimally invasive procedures.
Simuliidae is a family of small, robust two-winged flies known as black flies or buffalo gnats. The term "Simuliidae" itself is the taxonomic name for this group of insects. They are called black flies because many species are dark in color, and they are often referred to as gnats or buffalo gnats due to their small size and annoying biting behavior.
Black flies are well-known for their medical significance, as they can transmit several diseases to humans and animals, including onchocerciasis (river blindness), leucocytozoonosis, and various forms of bacterial infections. The female black flies feed on blood from hosts, while males primarily feed on nectar.
These insects are typically found near bodies of water, where their larvae develop in flowing or standing waters with high oxygen levels. They have aquatic habits and undergo a complete metamorphosis during their life cycle, transforming from an egg to larva, then pupa, and finally into an adult fly.
In summary, Simuliidae is the medical term for black flies or buffalo gnats, which are small, robust two-winged flies with a medical significance due to their ability to transmit diseases to humans and animals.
Medical Definition:
Murine leukemia virus (MLV) is a type of retrovirus that primarily infects and causes various types of malignancies such as leukemias and lymphomas in mice. It is a complex genus of viruses, with many strains showing different pathogenic properties.
MLV contains two identical single-stranded RNA genomes and has the ability to reverse transcribe its RNA into DNA upon infection, integrating this proviral DNA into the host cell's genome. This is facilitated by an enzyme called reverse transcriptase, which MLV carries within its viral particle.
The virus can be horizontally transmitted between mice through close contact with infected saliva, urine, or milk. Vertical transmission from mother to offspring can also occur either in-utero or through the ingestion of infected breast milk.
MLV has been extensively studied as a model system for retroviral pathogenesis and tumorigenesis, contributing significantly to our understanding of oncogenes and their role in cancer development. It's important to note that Murine Leukemia Virus does not infect humans.
Complementary DNA (cDNA) is a type of DNA that is synthesized from a single-stranded RNA molecule through the process of reverse transcription. In this process, the enzyme reverse transcriptase uses an RNA molecule as a template to synthesize a complementary DNA strand. The resulting cDNA is therefore complementary to the original RNA molecule and is a copy of its coding sequence, but it does not contain non-coding regions such as introns that are present in genomic DNA.
Complementary DNA is often used in molecular biology research to study gene expression, protein function, and other genetic phenomena. For example, cDNA can be used to create cDNA libraries, which are collections of cloned cDNA fragments that represent the expressed genes in a particular cell type or tissue. These libraries can then be screened for specific genes or gene products of interest. Additionally, cDNA can be used to produce recombinant proteins in heterologous expression systems, allowing researchers to study the structure and function of proteins that may be difficult to express or purify from their native sources.
A viral genome is the genetic material (DNA or RNA) that is present in a virus. It contains all the genetic information that a virus needs to replicate itself and infect its host. The size and complexity of viral genomes can vary greatly, ranging from a few thousand bases to hundreds of thousands of bases. Some viruses have linear genomes, while others have circular genomes. The genome of a virus also contains the information necessary for the virus to hijack the host cell's machinery and use it to produce new copies of the virus. Understanding the genetic makeup of viruses is important for developing vaccines and antiviral treatments.
Viral proteins are the proteins that are encoded by the viral genome and are essential for the viral life cycle. These proteins can be structural or non-structural and play various roles in the virus's replication, infection, and assembly process. Structural proteins make up the physical structure of the virus, including the capsid (the protein shell that surrounds the viral genome) and any envelope proteins (that may be present on enveloped viruses). Non-structural proteins are involved in the replication of the viral genome and modulation of the host cell environment to favor viral replication. Overall, a thorough understanding of viral proteins is crucial for developing antiviral therapies and vaccines.
Thymidine kinase (TK) is an enzyme that plays a crucial role in the synthesis of thymidine triphosphate (dTMP), a nucleotide required for DNA replication and repair. It catalyzes the phosphorylation of thymidine to thymidine monophosphate (dTMP) by transferring a phosphate group from adenosine triphosphate (ATP).
There are two major isoforms of thymidine kinase in humans: TK1 and TK2. TK1 is primarily found in the cytoplasm of proliferating cells, such as those involved in the cell cycle, while TK2 is located mainly in the mitochondria and is responsible for maintaining the dNTP pool required for mtDNA replication and repair.
Thymidine kinase activity has been used as a marker for cell proliferation, particularly in cancer cells, which often exhibit elevated levels of TK1 due to their high turnover rates. Additionally, measuring TK1 levels can help monitor the effectiveness of certain anticancer therapies that target DNA replication.
Terminal repeat sequences (TRS) are repetitive DNA sequences that are located at the termini or ends of chromosomes, plasmids, and viral genomes. They play a significant role in various biological processes such as genome replication, packaging, and integration. In eukaryotic cells, telomeres are the most well-known TRS, which protect the chromosome ends from degradation, fusion, and other forms of DNA damage.
Telomeres consist of repetitive DNA sequences (5'-TTAGGG-3' in vertebrates) that are several kilobases long, associated with a set of shelterin proteins that protect them from being recognized as double-strand breaks by the DNA repair machinery. With each cell division, telomeres progressively shorten due to the end replication problem, which can ultimately lead to cellular senescence or apoptosis.
In contrast, prokaryotic TRS are often found at the ends of plasmids and phages and are involved in DNA replication, packaging, and integration into host genomes. For example, the attP and attB sites in bacteriophage lambda are TRS that facilitate site-specific recombination during integration and excision from the host genome.
Overall, terminal repeat sequences are essential for maintaining genome stability and integrity in various organisms, and their dysfunction can lead to genomic instability, disease, and aging.
Bacterial transformation is a natural process by which exogenous DNA is taken up and incorporated into the genome of a bacterial cell. This process was first discovered in 1928 by Frederick Griffith, who observed that dead virulent bacteria could transfer genetic material to live avirulent bacteria, thereby conferring new properties such as virulence to the recipient cells.
The uptake of DNA by bacterial cells typically occurs through a process called "competence," which can be either naturally induced under certain environmental conditions or artificially induced in the laboratory using various methods. Once inside the cell, the exogenous DNA may undergo recombination with the host genome, resulting in the acquisition of new genes or the alteration of existing ones.
Bacterial transformation has important implications for both basic research and biotechnology. It is a powerful tool for studying gene function and for engineering bacteria with novel properties, such as the ability to produce valuable proteins or degrade environmental pollutants. However, it also poses potential risks in the context of genetic engineering and biocontainment, as transformed bacteria may be able to transfer their newly acquired genes to other organisms in the environment.
Spumavirus is actually referred to as " foamy virus" in medical terminology. It's a type of retrovirus, which means it uses RNA as its genetic material and has the ability to integrate its genetic material into the DNA of the host cell.
Spumaviruses are unique among retroviruses because they don't cause the same kind of diseases that other retroviruses do, like HIV. Instead, they're associated with a slow-growing, non-cancerous infection in various animal species, including cats and non-human primates. They're called "foamy viruses" because of the foamy or bubbly appearance of the infected cells when viewed under a microscope.
It's important to note that while spumaviruses can infect human cells in laboratory experiments, there's no evidence that they cause disease in humans.
DNA Sequence Analysis is the systematic determination of the order of nucleotides in a DNA molecule. It is a critical component of modern molecular biology, genetics, and genetic engineering. The process involves determining the exact order of the four nucleotide bases - adenine (A), guanine (G), cytosine (C), and thymine (T) - in a DNA molecule or fragment. This information is used in various applications such as identifying gene mutations, studying evolutionary relationships, developing molecular markers for breeding, and diagnosing genetic diseases.
The process of DNA Sequence Analysis typically involves several steps, including DNA extraction, PCR amplification (if necessary), purification, sequencing reaction, and electrophoresis. The resulting data is then analyzed using specialized software to determine the exact sequence of nucleotides.
In recent years, high-throughput DNA sequencing technologies have revolutionized the field of genomics, enabling the rapid and cost-effective sequencing of entire genomes. This has led to an explosion of genomic data and new insights into the genetic basis of many diseases and traits.
A larva is a distinct stage in the life cycle of various insects, mites, and other arthropods during which they undergo significant metamorphosis before becoming adults. In a medical context, larvae are known for their role in certain parasitic infections. Specifically, some helminth (parasitic worm) species use larval forms to infect human hosts. These invasions may lead to conditions such as cutaneous larva migrans, visceral larva migrans, or gnathostomiasis, depending on the specific parasite involved and the location of the infection within the body.
The larval stage is characterized by its markedly different morphology and behavior compared to the adult form. Larvae often have a distinct appearance, featuring unsegmented bodies, simple sense organs, and undeveloped digestive systems. They are typically adapted for a specific mode of life, such as free-living or parasitic existence, and rely on external sources of nutrition for their development.
In the context of helminth infections, larvae may be transmitted to humans through various routes, including ingestion of contaminated food or water, direct skin contact with infective stages, or transmission via an intermediate host (such as a vector). Once inside the human body, these parasitic larvae can cause tissue damage and provoke immune responses, leading to the clinical manifestations of disease.
It is essential to distinguish between the medical definition of 'larva' and its broader usage in biology and zoology. In those fields, 'larva' refers to any juvenile form that undergoes metamorphosis before reaching adulthood, regardless of whether it is parasitic or not.
Gene expression regulation, viral, refers to the processes that control the production of viral gene products, such as proteins and nucleic acids, during the viral life cycle. This can involve both viral and host cell factors that regulate transcription, RNA processing, translation, and post-translational modifications of viral genes.
Viral gene expression regulation is critical for the virus to replicate and produce progeny virions. Different types of viruses have evolved diverse mechanisms to regulate their gene expression, including the use of promoters, enhancers, transcription factors, RNA silencing, and epigenetic modifications. Understanding these regulatory processes can provide insights into viral pathogenesis and help in the development of antiviral therapies.
A capsid is the protein shell that encloses and protects the genetic material of a virus. It is composed of multiple copies of one or more proteins that are arranged in a specific structure, which can vary in shape and symmetry depending on the type of virus. The capsid plays a crucial role in the viral life cycle, including protecting the viral genome from host cell defenses, mediating attachment to and entry into host cells, and assisting with the assembly of new virus particles during replication.
An algorithm is not a medical term, but rather a concept from computer science and mathematics. In the context of medicine, algorithms are often used to describe step-by-step procedures for diagnosing or managing medical conditions. These procedures typically involve a series of rules or decision points that help healthcare professionals make informed decisions about patient care.
For example, an algorithm for diagnosing a particular type of heart disease might involve taking a patient's medical history, performing a physical exam, ordering certain diagnostic tests, and interpreting the results in a specific way. By following this algorithm, healthcare professionals can ensure that they are using a consistent and evidence-based approach to making a diagnosis.
Algorithms can also be used to guide treatment decisions. For instance, an algorithm for managing diabetes might involve setting target blood sugar levels, recommending certain medications or lifestyle changes based on the patient's individual needs, and monitoring the patient's response to treatment over time.
Overall, algorithms are valuable tools in medicine because they help standardize clinical decision-making and ensure that patients receive high-quality care based on the latest scientific evidence.
In the field of medicine, "time factors" refer to the duration of symptoms or time elapsed since the onset of a medical condition, which can have significant implications for diagnosis and treatment. Understanding time factors is crucial in determining the progression of a disease, evaluating the effectiveness of treatments, and making critical decisions regarding patient care.
For example, in stroke management, "time is brain," meaning that rapid intervention within a specific time frame (usually within 4.5 hours) is essential to administering tissue plasminogen activator (tPA), a clot-busting drug that can minimize brain damage and improve patient outcomes. Similarly, in trauma care, the "golden hour" concept emphasizes the importance of providing definitive care within the first 60 minutes after injury to increase survival rates and reduce morbidity.
Time factors also play a role in monitoring the progression of chronic conditions like diabetes or heart disease, where regular follow-ups and assessments help determine appropriate treatment adjustments and prevent complications. In infectious diseases, time factors are crucial for initiating antibiotic therapy and identifying potential outbreaks to control their spread.
Overall, "time factors" encompass the significance of recognizing and acting promptly in various medical scenarios to optimize patient outcomes and provide effective care.
Dengue is a mosquito-borne viral infection that is primarily transmitted by the Aedes aegypti and Aedes albopictus species of mosquitoes. It is caused by one of four closely related dengue viruses (DENV 1, DENV 2, DENV 3, or DENV 4). The infection can cause a wide range of symptoms, ranging from mild fever and headache to severe flu-like illness, which is often characterized by the sudden onset of high fever, severe headache, muscle and joint pain, nausea, vomiting, and skin rash. In some cases, dengue can progress to more severe forms, such as dengue hemorrhagic fever or dengue shock syndrome, which can be life-threatening if not treated promptly and appropriately.
Dengue is prevalent in many tropical and subtropical regions around the world, particularly in urban and semi-urban areas with poor sanitation and inadequate mosquito control. There is no specific treatment for dengue, and prevention efforts focus on reducing mosquito populations and avoiding mosquito bites. Vaccines are available in some countries to prevent dengue infection, but they are not widely used due to limitations in their effectiveness and safety.
Viral genes refer to the genetic material present in viruses that contains the information necessary for their replication and the production of viral proteins. In DNA viruses, the genetic material is composed of double-stranded or single-stranded DNA, while in RNA viruses, it is composed of single-stranded or double-stranded RNA.
Viral genes can be classified into three categories: early, late, and structural. Early genes encode proteins involved in the replication of the viral genome, modulation of host cell processes, and regulation of viral gene expression. Late genes encode structural proteins that make up the viral capsid or envelope. Some viruses also have structural genes that are expressed throughout their replication cycle.
Understanding the genetic makeup of viruses is crucial for developing antiviral therapies and vaccines. By targeting specific viral genes, researchers can develop drugs that inhibit viral replication and reduce the severity of viral infections. Additionally, knowledge of viral gene sequences can inform the development of vaccines that stimulate an immune response to specific viral proteins.
A cell line that is derived from tumor cells and has been adapted to grow in culture. These cell lines are often used in research to study the characteristics of cancer cells, including their growth patterns, genetic changes, and responses to various treatments. They can be established from many different types of tumors, such as carcinomas, sarcomas, and leukemias. Once established, these cell lines can be grown and maintained indefinitely in the laboratory, allowing researchers to conduct experiments and studies that would not be feasible using primary tumor cells. It is important to note that tumor cell lines may not always accurately represent the behavior of the original tumor, as they can undergo genetic changes during their time in culture.
Chagas disease, also known as American trypanosomiasis, is a tropical parasitic disease caused by the protozoan *Trypanosoma cruzi*. It is primarily transmitted to humans through the feces of triatomine bugs (also called "kissing bugs"), which defecate on the skin of people while they are sleeping. The disease can also be spread through contaminated food or drink, during blood transfusions, from mother to baby during pregnancy or childbirth, and through organ transplantation.
The acute phase of Chagas disease can cause symptoms such as fever, fatigue, body aches, headache, rash, loss of appetite, diarrhea, and vomiting. However, many people do not experience any symptoms during the acute phase. After several weeks or months, most people enter the chronic phase of the disease, which can last for decades or even a lifetime. During this phase, many people do not have any symptoms, but about 20-30% of infected individuals will develop serious cardiac or digestive complications, such as heart failure, arrhythmias, or difficulty swallowing.
Chagas disease is primarily found in Latin America, where it is estimated that around 6-7 million people are infected with the parasite. However, due to increased travel and migration, cases of Chagas disease have been reported in other parts of the world, including North America, Europe, and Asia. There is no vaccine for Chagas disease, but medications are available to treat the infection during the acute phase and to manage symptoms during the chronic phase.
Viral vector
Shuttle vector
Expression vector
Lentiviral vector in gene therapy
Taenia asiatica
Taeniasis
Poskam County, Xinjiang
Onchocerca lupi
Viral vector vaccine
Diplostomum pseudospathaceum
Pelagia noctiluca
Transfer DNA binary system
Monica Asman
Promoter (genetics)
Plasmid
Peter Palese
Lentivirus
Duffy antigen system
Gerald M. Rubin
Allan C. Spradling
Gene drive
Viral diseases of potato
Insertional mutagenesis
Genetically modified animal
Biomphalaria choanomphala
Biomphalaria sudanica
Cloning vector
Phaeodactylum tricornutum
Sterile insect technique
Centre for Neuroscience and Cell Biology
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Viral24
- Is the Subject Area "Viral vectors" applicable to this article? (plos.org)
- Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells. (wikipedia.org)
- In addition to their use in molecular biology research, viral vectors are used for gene therapy and the development of vaccines. (wikipedia.org)
- 50 Viral Vectors are tailored to their specific applications but generally share a few key properties. (wikipedia.org)
- Safety: Although viral vectors are occasionally created from pathogenic viruses, they are modified in such a way as to minimize the risk of handling them. (wikipedia.org)
- Low toxicity: The viral vector should have a minimal effect on the physiology of the cell it infects. (wikipedia.org)
- This is detrimental to predictability and reproducibility of the work conducted using a viral vector and is avoided in their design. (wikipedia.org)
- Cell type specificity: Most viral vectors are engineered to infect as wide a range of cell types as possible. (wikipedia.org)
- The viral vector can be modified to target the virus to a specific kind of cell. (wikipedia.org)
- Identification: Viral vectors are often given certain genes that help identify which cells took up the viral genes. (wikipedia.org)
- The cells can then be isolated easily, as those that have not taken up the viral vector genes do not have antibiotic resistance, and so cannot grow in a culture with the relevant antibiotic present. (wikipedia.org)
- Viral vectors were originally developed as an alternative to transfection of naked DNA for molecular genetics experiments. (wikipedia.org)
- Protein coding genes can be expressed using viral vectors, commonly to study the function of the particular protein. (wikipedia.org)
- Viral vectors, especially retroviruses, stably expressing marker genes such as GFP are widely used to permanently label cells to track them and their progeny, for example in xenotransplantation experiments, when cells infected in vitro are implanted into a host animal. (wikipedia.org)
- Gene insertion, which can be done with viral vectors, is cheaper to carry out than gene knockout. (wikipedia.org)
- Some viral vectors, for instance gamma-retroviruses, insert their genomes at a seemingly random location on one of the host chromosomes, which can disturb the function of cellular genes and lead to cancer. (wikipedia.org)
- A synthetic viral vector and a novel surgical approach developed at Mass Eye and Ear has enabled efficient gene transfer into the inner ear of nonhuman primates, a research team working towards the development of effective treatments for hearing loss and vestibular disorders has reported. (scienmag.com)
- Once inside the inner ear, the gene therapy is delivered via a viral vector. (scienmag.com)
- What is particularly interesting about AAVHSCs, is that they all map to Clade F (same viral family) but the genetic differences they have lead to different biodistribution profiles. (homologymedicines.com)
- There are a number of alternate vaccination strategies in current development which may circumvent the need for annual re-vaccination, including new platform technologies such as viral-vectored vaccines. (mdpi.com)
- We discuss the different vectored vaccines that have been or are currently in clinical trials, with a forward-looking focus on immunogens that may be protective against seasonal and pandemic influenza infection, in the context of viral-vectored vaccines. (mdpi.com)
- Adenovirus (Ad) vectors are among the most commonly used viral vectors in gene therapy clinical trials because they have the following advantages. (hindawi.com)
- i) They can achieve a 1000-fold higher-titer vector stock compared with other viral vectors, such as adeno-associated virus, retrovirus, and lentivirus vectors, at an equivalent scale. (hindawi.com)
- Viral vector vaccines use genetic material, which gives your cells instructions for making a protein of the germ. (medlineplus.gov)
Aedes6
- In February 2004, the discovery of an incursion of the dengue vector Aedes aegypti into the town of Tennant Creek in the Northern Territory caused concern for the Northern Territory health authorities who proceeded to implement a Commonwealth-funded eradication program. (health.gov.au)
- Aedes aegypti is the primary vector of dengue virus. (health.gov.au)
- Aedes albopictus is a mosquito that has taken advantage of human activities to extend its territory and be linked to the emergence of arbovirus of which it is the vector. (pasteur.fr)
- Vector Competence of Aedes caspius and Ae. (cdc.gov)
- 2 The most important dengue vector in the Western Pacific Region is Aedes aegypti ( Stegomyia aegypti ), which is predominately found in densely populated urban areas. (who.int)
- Dengue outbreaks have also been associated with Aedes albopictus , particularly in China, 3 and several other Aedes species found in South Pacific countries and areas may also be competent dengue vectors. (who.int)
Parasites3
- Here, we review the arrival of genetic screens in malaria parasites to analyse parasite gene function at a genome-scale and their impact on understanding parasite biology. (portlandpress.com)
- CRISPR/Cas9 screens, which have revolutionised human and model organism research, have not yet been implemented in malaria parasites due to the need for more complex CRISPR/Cas9 gene targeting vector libraries. (portlandpress.com)
- We therefore introduce the reader to CRISPR-based screens in the related apicomplexan Toxoplasma gondii and discuss how these approaches could be adapted to develop CRISPR/Cas9 based genome-scale genetic screens in malaria parasites. (portlandpress.com)
Populations14
- This genetic marker was also useful in identifying a separate Townsville haplotype population and another population on Thursday Island in the Torres Strait that is genetically distant to the mainland populations. (health.gov.au)
- Identifying differences in mosquito strains or populations requires a DNA-based genetic marker that will be informative, will deliver an unambiguous result, will be relatively straightforward to use, and ideally, be useful in later studies of evolution or population genetics. (health.gov.au)
- argued that the populations of C. parvum in the UK were panmictic, based on genetic diversity whereas those of C. hominis are more clonal [ 9 ]. (biomedcentral.com)
- albopictus has shaped the vector competence of these populations. (pasteur.fr)
- albopictus, which results from successive events of invasion, diversification and divergence from ancestral lines, has shaped the vector competence of these populations. (pasteur.fr)
- mosquito populations from Europe are screening legs, and transmission rates by screening saliva, competent vectors for Zika virus ( 3,4 ). (cdc.gov)
- Commonly, control strategies for vector-borne plant pathogens focus on suppression of vector populations. (utrgv.edu)
- Genetic variation studies in pest populations provide useful information for biological control, understanding pesticide resistance, and inferring global movement patterns. (utrgv.edu)
- In chapter I, genetic variation of worldwide populations of Diaphorina citri , the vector of Citrus greening disease, was examined to assess potential sites of origin of invasive populations. (utrgv.edu)
- To better understand the basis for this increase, more data on the geographic distribution of the vector populations are needed. (biomedcentral.com)
- To our knowledge, Lent-On-Plus is the first all-in-one vector system that tightly regulates transgene expression in bulk populations of human pluripotent stem cells and its progeny. (nih.gov)
- The haplotype analyses showed higher intrapopulation genetic divergence in northern Peruvian Andes populations and less divergence in the southern Peru and Ecuador populations, suggesting that a population bottleneck occurred in the latter populations, but not in former ones. (edu.pe)
- Our data suggests that targeting the genetic viability of rat populations may be another important component for integrated pest management (IPM) strategies, designed to reduce urban rats. (frontiersin.org)
- Several findings promoted experimental BLU research (3,10): the showing of the plurality of strains (23), the successful use of the propagation of BLU inembryonated chicken eggs(1), the development of cell culture as a neutralization assay system (8), and the demonstration for wild and colonized populations of C. variipennis that this species was a biological vector of bluetongue virus(7). (cdc.gov)
Lentiviral vectors3
- In the present study, we aim the generation of Tet-On all-in-one lentiviral vectors (LVs) that tightly regulate transgene expression in human stem cells using the original TetR repressor. (nih.gov)
- Lentiviral vectors (LVs) are considered one of the most promising vehicles to efficiently deliver genetic information for basic research and gene therapy approaches. (nih.gov)
- In the present manuscript we have constructed a dual Tet-ON system based on two lentiviral vectors, one expressing the TetR through the spleen focus forming virus (SFFV) promoter (STetR) and a second expressing eGFP through the regulatable CMV-TetO promoter (CTetOE). (nih.gov)
Adeno-assoc2
- Adeno-associated virus-based vectors are much safer in this respect as they always integrate at the same site in the human genome, with applications in various disorders, such as Alzheimer's disease. (wikipedia.org)
- The new findings, published March 15 in Nature Communications , suggest that an adeno-associated virus (AAV) vector called Anc80L65 can be combined with a novel surgical approach, consisting of a transmastoid posterior tympanotomy, to deliver genes into the inner ear, which could provide a promising pathway for translating this research into humans. (scienmag.com)
Arthropod Vectors2
- Arthropod vectors are agriculturally and ecologically important because they transmit numerous plant pathogens. (utrgv.edu)
- Among arthropod vectors , ticks transmit the most diverse human and animal pathogens, leading to an increasing number of new challenges worldwide. (bvsalud.org)
Genomes4
- We have also showed that this dual vector system can generate regulatable bulk cell lines (expressing high levels of TetR) that are able to modulate transgene expression either by varying doxycycline concentration and/or by varying the amount of CTetOE vector genomes per cell. (nih.gov)
- Large-Scale Comparative Analyses of Tick Genomes Elucidate Their Genetic Diversity and Vector Capacities. (bvsalud.org)
- Here we sequenced and assembled high-quality genomes of six ixodid tick species and further resequenced 678 tick specimens to understand three key aspects of ticks genetic diversity , population structure, and pathogen distribution. (bvsalud.org)
- New classes of diagnostics, vaccines and therapeutic agents, and new approaches to vector control can derive from the study of human, pathogen and vector genomes. (who.int)
Malaria vectors2
- Malaria control in Somaliland depends on the effective identification of potential malaria vectors, particularly those that may be invasive. (biomedcentral.com)
- Resistance in malaria vectors to pyrethroids, the most widely used class of insecticides for malaria vector control, threatens the continued efficacy of vector control tools. (lstmed.ac.uk)
Algorithm3
- A novel variable-rate vector quantizer (VQ) design algorithm, which is a hybrid approach combining a genetic algorithm with the entropy-constrained VQ (ECVQ) algorithm is presented. (ntnu.edu.tw)
- Thus, a hybrid model comprising least squares support vector machine (LSSVM) and genetic algorithm (GA) was developed in this work to predict electricity prices with higher accuracy. (utm.my)
- Because SVM has a weakness in feature selection during classification, the SVM algorithm optimization feature is implemented using feature selection, with Particle Swarm Optimization (PSO) and Genetic Algorithm (GA). Tweets taken were 839 data tweets, then divided into 640 data for the training process and 199 data for the testing process. (uksw.edu)
Major malaria vector1
- We develop a Bayesian statistical spatiotemporal model to interpret species-specific trends in the frequency of the most common resistance mutations, Vgsc-995S and Vgsc- 995F, in three major malaria vector species Anopheles gambiae, An. (lstmed.ac.uk)
Arboviruses1
- 220 897 dengue fever cases, and 25 064 Rift Valley fever cases among arboviruses or vector-borne diseases reported in 2020. (who.int)
Humans2
- Together, these observations suggest that genetic inhibition of ANGPTL4 function might have a favorable impact on glucose homeostasis in humans and reduce risk of type 2 diabetes. (nature.com)
- Genetic changes in microbes, animal vectors, and humans were included. (cdc.gov)
Diversity8
- The hypothesis that genetic diversity at GP60 locus mirrors notification rates of human cryptosporidiosis was not rejected based on the data presented. (biomedcentral.com)
- Genetic diversity of C. hominis and C. parvum may therefore be an independent and complementary measure for quantifying disease incidence, for which only a moderate number of stool samples from each nation are sufficient data input. (biomedcentral.com)
- Here, we test the hypothesis that the genetic diversity of Cryptosporidium at the GP60 locus from human isolates positively correlates with the population risk. (biomedcentral.com)
- These communities showed low genetic diversity, lack of association with Citrus species, and a high proportion of disease vectors. (utrgv.edu)
- The malaria vector Anopheles stephensi has been detected in multiple countries in the Horn of Africa (HOA), but data on its geographic distribution and population genetic diversity are incomplete. (biomedcentral.com)
- genetic diversity revealed three cytochrome oxidase I ( COI ) haplotypes, all of which have been observed in other countries in East Africa and one also observed in South Asia. (biomedcentral.com)
- Genetic Diversity among Lathyrus ssp. (mdpi.com)
- Epidemiology and genetic diversity of Taenia asiatica: a systematic review. (msdmanuals.com)
Vaccines1
- These vaccines also contain a different, harmless virus that helps get the genetic material into your cells. (medlineplus.gov)
Dengue8
- It is the only dengue vector in mainland Australia and has been responsible for outbreaks of dengue fever that reappeared in northern Queensland in the early 1980s and have continued until the present. (health.gov.au)
- In the absence of a vaccine, vector control is the mainstay for dengue prevention and control. (who.int)
- These include Release of Insects Carrying a Dominant Lethal system and Wolbachia , an endosymbiotic bacterium, to inhibit dengue virus in the vector. (who.int)
- Given the lack of a dengue vaccine, control of dengue depends on vector control. (who.int)
- The primary goal of vector control activities is to reduce vector population density to levels that are believed to correlate with a lower dengue transmission risk. (who.int)
- In this paper we describe dengue vector surveillance and control practices in the Western Pacific Region and provide a perspective for future dengue control. (who.int)
- While insufficient to accurately predict the risk of human infection, dengue vector surveillance employs several entomological indicators that have been developed to assess the risk of outbreaks occurring. (who.int)
- 5,6 A model employing all three parameters of dengue transmission, vector density, human cases and vector infection rate, would probably be the most accurate in determining outbreak thresholds for early outbreak prediction. (who.int)
Transduction4
- Delivery of genes or other genetic material by a vector is termed transduction and the infected cells are described as transduced. (wikipedia.org)
- however, the transduction properties of Ad vectors in neonates and infants have not been fully examined. (hindawi.com)
- Accordingly, this study examined the properties of Ad vector-mediated transduction in neonatal mice. (hindawi.com)
- On the other hand, Ad vectors also have a number of disadvantages, including their low transduction efficiency in cells lacking coxsackievirus-adenovirus receptor (CAR) and their induction of innate immune responses. (hindawi.com)
Particle swarm optimi2
- R. Darmawan, I. Indra, and A. Surahmat, "Optimalisasi Support Vector Machine (SVM) Berbasis Particle Swarm Optimization (PSO) Pada Analisis Sentimen Terhadap Official Account Ruang Guru di Twitter," J. Kaji. (uksw.edu)
- V. K. S. Que, A. Iriani, and H. D. Purnomo, "Analisis Sentimen Transportasi Online Menggunakan Support Vector Machine Berbasis Particle Swarm Optimization," J. Nas. (uksw.edu)
Mosquitoes3
- Image of mosquito from http://bloodinurineandpainofca.wordpress.com/ Until now I thought I had come up with enough reasons to dislike mosquitoes, those tiny little blood sucking vectors of disease. (scienceblogs.com)
- Conclusions/Significance Our results highlight the importance of genetic research of invasive mosquitoes to understand general dispersal patterns, reveal main dispersal routes and form the baseline of future mitigation actions. (fmach.it)
- The use of insecticides targeting larvae and adult mosquitoes remains the mainstay of vector control programmes. (who.int)
Modification3
- More than half (23 of 39) of the articles represent epidemiologic studies including candidate gene and genome-wide association studies (GWAS) examining genetic effect modification on disease risk. (cdc.gov)
- Genetic modification of plants offers the potential to influence plant growth, fertility and disease resistance - thus impacting on food security and nutritional status. (who.int)
- The lab consists of molecular tools that can be used for genetic modification of neurons. (lu.se)
Liposomes2
- The second step is to insert the insulin gene into the vector.Viruses, liposomes (hollow spheres of fat molecules formed in solution), andplasmids (circular forms of DNA) are common vectors. (faqs.org)
- Viruses, liposomes, and other vectors of all kinds are being used to usher new genes into cells, correcting faulty copies or equipping a cell to fight disease. (medscape.com)
Genomic2
- Considering its public health importance, it is crucial to further investigate the species' population genetic dynamic, including a larger sampling and additional genomic markers. (fmach.it)
- Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning[J].Proc Natl Acad Sci USA,1999,96(11):6535-6540. (nefu.edu.cn)
Species8
- is an exotic mosquito that probably arrived in Australia during the mid-19th century, 10 a rapidly evolving genetic marker would be required to identify population variation within this species. (health.gov.au)
- In chapter II, I explored the community of potential Citrus leprosis vectors, Brevipalpus species, on Citrus plants. (utrgv.edu)
- Finally, a genetic expansion signal was identified, suggesting the species might be present in more locations than currently detected. (fmach.it)
- Understanding the geographic distribution of target-site resistance, and temporal trends across different vector species, can inform strategic deployment of vector control tools. (lstmed.ac.uk)
- The results show associations, as well as stark differences, in spread dynamics of the two mutations across the three vector species. (lstmed.ac.uk)
- gambiae vary across individual vector species and geographic regions. (lstmed.ac.uk)
- We explored the genetic basis common to ticks , including heme and hemoglobin digestion , iron metabolism , and reactive oxygen species , and unveiled for the first time that genetic structure and pathogen composition in different tick species are mainly shaped by ecological and geographic factors . (bvsalud.org)
- This is the first finding of natural infection of P. kandelakii by L. infantum in this region suggesting P. kandelakii may be the vector of L. infantum in the area although it is the second most prevalent phlebotomine species. (who.int)
Transmit1
- In the 1950s, largely as the result of the pioneering work of James Watson (1928-) and Francis Crick (1916-), scientists discovered the structure of deoxyribonucleic acid (DNA) molecules and how DNA stores and transmit genetic information. (faqs.org)
Competent vector1
- They can consolidate elimination gains by providing barriers to parasite and competent vector reintroduction, and allow resources to be focused on new sites while providing confidence that treated areas will remain malaria-free. (ox.ac.uk)
Chikungunya virus2
- albopictus vector competence for chikungunya virus through an adaptive co-evolution process. (pasteur.fr)
- We potential vector of chikungunya virus [ 10 ] and Rift Valley conducted 3 independent trials using different Zika virus fever virus [ 11 ]), indicate a risk for Zika virus transmission concentrations at different time points (7, 14, or 21 dpi) for in Spain. (cdc.gov)
Transgene expression1
- The highest Ad vector genome copy numbers and transgene expression were found in the neonatal liver. (hindawi.com)
Disease7
- When used therapeutically, the AAVs do not cause disease, but instead efficiently carry genetic material to tissues and organs inside the body. (homologymedicines.com)
- Huntington's disease is an example of an autosomal dominant genetic disorder. (genome.gov)
- Over the next month, a highly effective collaboration ensued between the Indian Health Service, the University of New Mexico School of Medicine, and the Centers for Disease Control and Prevention, leading not only to the successful isolation of the virus, but also to the identification of the reservoir and vector for the disease, Peromyscus maniculatus (deer mouse). (medscape.com)
- Occupational exposures involving crystalline silica and multiwalled carbon nanotubes were also studied in relation to genetic damage and initiation of disease processes. (cdc.gov)
- and strengthen prevention of tropical and vector-borne disease outbreaks in epidemic-prone Member States by 2030. (who.int)
- She has prepped 24 different pediatricians and family doctors to deliver the news: A seemingly perfect newborn likely has a lethal genetic disease. (medscape.com)
- The vectors are used in the production of disease models, experimental gene therapy and basic experimental research of brain functions. (lu.se)
Anopheles6
- Background Anopheles darlingi is the main malaria mosquito vector in the Amazonia region. (iscte-iul.pt)
- Anopheles gambiae , one of the primary vectors of malaria in Africa, breeds in numerous small pools of water that form due to rainfall. (cdc.gov)
- We implemented a vector surveillance program and performed molecular analysis of Anopheles in three urban areas in Somaliland. (biomedcentral.com)
- and the long-established HOA malaria vector Anopheles arabiensis . (biomedcentral.com)
- While significant progress has been made to reduce malaria cases and mortality over the last decade, the recent detection of a new vector, Anopheles stephensi, in the Horn of Africa (HOA) threatens to reverse this progress. (biomedcentral.com)
- Anopheles stephensi , a vector found in South Asia and the Middle East, including large parts of the Arabian Peninsula, was first detected in Djibouti in 2012 [ 2 ]. (biomedcentral.com)
Genome4
- Vectors can either integrate into a cell's genome or transiently express a gene with non-integrative vectors. (wikipedia.org)
- For gene editing, our AAVHSC vectors are designed with long stretches of DNA, or homology arms, which guide a gene to the exact location in the genome where it is needed to integrate. (homologymedicines.com)
- Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic engineering tool in various biological systems. (mdpi.com)
- iii) They do not exhibit genetic toxicity because they do not integrate the transgene into the host genome. (hindawi.com)
Genes3
- In the future, gene therapy may provide a way to cure genetic disorders, such as severe combined immunodeficiency, cystic fibrosis or even haemophilia A. Because these diseases result from mutations in the DNA sequence for specific genes, gene therapy trials have used viruses to deliver unmutated copies of these genes to the cells of the patient's body. (wikipedia.org)
- More than half of all childhood deafness cases are genetic and caused by mutations in over 120 different genes, fueling the need for a gene therapy solution. (scienmag.com)
- When the genetic material within the living cells, i.e. genes are working properly, the human body can develop and function smoothly. (faqs.org)
Mutations1
- Target-site resistance is an important genetic resistance mechanism caused by mutations in the voltage-gated sodium channel (Vgsc) gene that encodes the pyrethroid target-site. (lstmed.ac.uk)
Promoter4
- The effects of a maize knotted1 ( kn1) gene under the control of the cauliflower mosaic virus 35S promoter on genetic transformation efficiencies of six citrus genotypes were tested. (springer.com)
- Based on these results we have developed a new all-in-one lentiviral vector (CEST) driving the expression of TetR through the SFFV promoter and the expression of eGFP through the doxycycline-responsive CMV-TetO operon. (nih.gov)
- Our gene therapy candidates utilize our AAVHSC vectors to deliver a functional gene and promoter to a cell where there is a missing or non-functional gene. (homologymedicines.com)
- A first-generation Ad vector containing a cytomegalovirus (CMV) promoter-driven luciferase expression cassette was administered to neonatal mice on the second day of life via retro-orbital sinus. (hindawi.com)
Parasite1
- La présente étude visait à identifier le(s) vecteur(s), le parasite et la composition des espèces des phlébotomes dans le district, de juillet à septembre 2009, à l'aide de techniques d'amplification en chaîne par polymérase. (who.int)
Surveillance3
- In this paper we describe vector surveillance and vector control in the Western Pacific countries and areas. (who.int)
- Vector surveillance and control strategies used by countries and areas of the Western Pacific Region vary. (who.int)
- Vector surveillance conducted using these and other indicators in several Western Pacific Region countries and areas ( Table 1 ). (who.int)
Disorders3
- At Homology, we have a lofty goal, which is to deliver to patients one-time, i n vivo (in the body) treatments for genetic disorders. (homologymedicines.com)
- Autosomal dominant is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
- Autosomal recessive is a pattern of inheritance characteristic of some genetic disorders. (genome.gov)
20221
- M. I. Petiwi, A. Triayudi, and I. D. Sholihati, "Analisis Sentimen Gofood Berdasarkan Twitter Menggunakan Metode Naïve Bayes dan Support Vector Machine," vol. 6, pp. 542-550, 2022, doi: 10.30865/mib.v6i1.3530. (uksw.edu)
Sequences4
- Based on 296 C. hominis and 195 C. parvum GP60 sequences from human origin, the genetic diversities of Cryptosporidium was estimated for several nations. (biomedcentral.com)
- In other words, the exposure to infectious oocysts in a human population could be indirectly measured by quantifying the genetic diversities of C. hominis and C. parvum DNA sequences, as found in human fecal samples. (biomedcentral.com)
- Mobile genetic elements (MGEs) constitute much of the repetitive component, particularly the interspersed sequences. (taylorfrancis.com)
- Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. (harvard.edu)
Functional3
- Genetic vectors possess a functional replicator site and contain genetic markers to facilitate their selective recognition. (usda.gov)
- We also evaluate the functional consequences of rare and novel genetic variants identified by exome sequencing, as well as the effect of Angptl4 deletion on insulin sensitivity and glucose homeostasis. (nature.com)
- This is due to a paucity of functional annotation from sequence homology, which is compounded by low genetic tractability compared with many model organisms. (portlandpress.com)
Gene transfer1
- The vector that transfers the gene therapy into the inner ear is called Anc80L65, which is an AAV first created in the laboratory of Dr. Vandenberghe and was previously shown in mouse studies to be a potent gene transfer vector to several organs. (scienmag.com)
Infect1
- This means that sexual recombination is likely to be common if strains from different genetic heritages infect the same animal or person. (biomedcentral.com)
Expression vector3
- The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. (lu.se)
- In order to explore the function of PI gene in the development of petals and stamens, the PI gene in Arabidopsis thaliana was used as the research object, and the AtPI gene was amplified from cDNA by PCR, and the plant expression vector(pROKⅡ- AtPI ) was constructed and transformed into tobacco. (nefu.edu.cn)
- Construction of Plant Expression Vector and Genetic Transformation Analysis of Arabidopsis thaliana AtPI Gene in Nicotiana tabacum [J]. Bulletin of Botanical Research, 2016, 36(3): 388-394. (nefu.edu.cn)
Mosquito1
- This information is essential to better estimate the risks of chikungunya emergence, specifying the strong genetic proximity of the mosquito in South-East France with that of La Reunion Island, where the largest chikungunya epidemic ever occurred in France, and more generally in Europe. (pasteur.fr)
Control8
- Our results demonstrate that expression of the kn1 gene enhances transformation efficiencies from 3 to 15 fold compared to a control vector, 3-11 fold relative to the highest transformation efficiencies reported for these citrus genotypes. (springer.com)
- We discuss the public health and ecological implications of these rapid genetic impacts of urban control efforts. (frontiersin.org)
- The integrated framework builds on progress made in the last two decades in the control, elimination and/or eradication of tropical and vector-borne diseases and addresses major programme deficiencies that drive the persistently high burden of these diseases and the lost momentum towards the 2030 targets. (who.int)
- Vector control strategies include chemical, biological and environmental management that mainly target larval breeding sites. (who.int)
- Existing vector control tools have several limitations in terms of cost, delivery and long-term sustainability. (who.int)
- Any vector control strategy should be selected based on evidence and appropriateness for the entomological and epidemiological setting and carried out in both inter-epidemic and epidemic periods. (who.int)
- Vector control is best achieved through management of breeding sites. (who.int)
- Vector control methods and strategies differ across countries and areas in the Western Pacific Region ( Table 2 ). (who.int)
Efficient2
- An efficient genetic transformation protocol is a key step for using transgenic technologies to improve citrus quality, productivity, and resistance to abiotic and biotic stresses such as HLB. (springer.com)
- ii) Neonates and infants have lower body weight than adults, and thus efficient therapeutic effects can be achieved at lower vector doses. (hindawi.com)
Support vector2
- Support Vector Machine (SVM) can classify sentiment analysis into positive or negative sentiment. (uksw.edu)
- H. C. Husada and A. S. Paramita, "Analisis Sentimen Pada Maskapai Penerbangan di Platform Twitter Menggunakan Algoritma Support Vector Machine (SVM)," Teknika, vol. 10, no. 1, pp. 18-26, 2021, doi: 10.34148/teknika.v10i1.311. (uksw.edu)
Physics1
- Maybe you forgot some of that stuff, so here are the bare essentials you will need to get by (this is assuming you are in the algebra-based second semester of physics) Vectors Really, just about the entire semester course is about the electric and magnetic field. (scienceblogs.com)
Clinical trials1
- Ad vectors have been used in gene therapy clinical trials of adult patients with various diseases, including several types of cancers and cardiac diseases, and promising results have been reported [ 9 , 10 ]. (hindawi.com)
Population genetic2
- Population genetic analysis with Mexican and Brazilian mites revealed no population structure suggesting that these vectors can migrate unhindered between these areas. (utrgv.edu)
- While the primary goal of these interventions is to reduce rat numbers and their detrimental activities, there are important, yet unexplored, population genetic implications for these rapid population declines. (frontiersin.org)