A Continuation Study of TH-CR-406/SARC021 for the Remaining Subjects Enrolled in TH-CR-406/SARC021 in the US - AdisInsight

To verify the assignment of performance of the unique viral gene, | Smad Pathway

Map Coordinates (APK+Mod) - APKNxt

Reference raster cells to map coordinates - MATLAB

ESTRUCTURA SARCOMERO PDF

Ichnovirus - Wikipedia

Kozak, Christine

Experimentally introduced defective endogenous proviruses are...

Virus Genes - incl. option to publish open access

Virus Genes - incl. option to publish open access

Regulators Discover a Hidden Viral Gene in Commercial GMO Crops : Conscious Life News

Virus Answers | Wyzant Resources

Function of herpes simplex virus gene products<...

UL30 - Uncharacterized protein UL30 - Human cytomegalovirus (strain Merlin) (HHV-5) - UL30 gene & protein

DtIV - Diadegma Terebrans Ichnovirus (virology) | AcronymFinder

The DNA Sequences of the Long Repeat Region and Adjoining Parts of the Long Unique Region in the Genome of Herpes Simplex Virus...

Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication

UL146 - Chemokine vCXCL1 - Human cytomegalovirus (strain Merlin) (HHV-5) - UL146 gene & protein

Publications | the Department of Immunobiology

mathematics - Staggered Isometric Map: Calculate map coordinates for point on screen - Game Development Stack Exchange

Researchers identify mechanism that could help old m... ( [Boston MA June 17 2014]─ Sarc...)

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The expression of the Autographa californica nuclear polyhedrosis virus genome in insect cells - Fingerprint - KAUST...

Identification and characterization of the virion-induced host shutoff product of herpes simplex virus gene UL41<...

High-efficiency nonhomologous insertion of a foreign gene into the herpes simplex virus genome | Microbiology Society

herpes simplex virus gene | The Source | Washington University in St. Louis

Characterization of single stranded viral DNA sequences present during replication of adenovirus types 2 and 5 - Fingerprint ...

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Are endogenous virus genes evidence for common descent? | WINTERY KNIGHT

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CAZy - Viruses

Monitoring of ganciclovir sensitivity of multiple human cytomegalovirus strains coinfecting blood of an AIDS patient by an...

5 viral and human cellular sequences corresponding to the transforming gene of simian sarcoma virus | Science

Linkage of Mls genes to endogenous mammary tumour viruses of inbred m by W N. Frankel, C Rudy et al.

CRISPR-Cas9 system-driven site-specific selection pressure on Herpes simplex virus genomes. | Sigma-Aldrich

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Presence of a novel recombinant murine leukemia virus-like glycoprotei by P J. Fischinger, H J. Thiel et al.

siRNA Treatment Slashes Viral Gene Expression by 80%

Hump day Hilarity - Chinas wind powered car | Watts Up With That?

Coordination of late gene transcription of human cytomegalovirus with viral DNA synthesis: recombinant viruses as potential...

High level expression of DNA polymerases from herpesviruses. - Semantic Scholar

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Synthesis of Long, Representative DNA Copies of the Murine RNA Tumor Virus Genome | Journal of Virology

Adenovirus early region 4 encodes functions required for efficient DNA replication, late gene expression, and host cell shutoff...

Dissociative electron capture of H2+ into H− fragments - Lancaster EPrints

Structural and functional characterization of the Fujinami sarcoma virus transforming protein - UBC Library Open Collections

REGULATION OF A BACTERIOPHAGE T4 LATE GENE, soc, WHICH MAPS IN AN EARLY REGION | Genetics

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Kenwood TK-3200 Compatible Accessories and Equipment

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Epitranscriptome

Murine norovirus

Cell fusion

Watchmen (film)

Oncolytic virus

DNA construct

Reoviridae

Magnet-assisted transfection

Ras GTPase

Pelareorep

P73

Frizzled-9

Lissencephaly

Podoviridae

Antigenic variation

Transfection

Polydnavirus

Suipoxvirus

RNA editing

Occult macular dystrophy

Apoptosis

Titer

Nanorobotics

TPSB2

Herpes simplex research

Human foamy virus

EVI2B

EVI5

EIF4A1

Agroinfiltration

Haemophilia A

Microcephaly

Interleukin 6

Protein-protein interaction

Species

Adaptive immune system

போலியோ வைரஸ் - தமிழ் விக்கிப்பீடியா

Angiotensin-converting enzyme 2

Neutropenia

Pseudofolliculitis barbae

Deoksiribonukleinska kislina - Wikipedija, prosta enciklopedija

Interferon alfa-2b

Capitol Hill Occupied Protest

Chitinase

普通感冒 - 维基百科，自由的百科全书

Variants del SARS-CoV-2 - Viquipèdia, l'enciclopèdia lliure

Substance dependence

Ferritin

Deep learning

Common cold - Simple English Wikipedia, the free encyclopedia

Cryptococcosis

DNA repair

Amylase

বংশাণু - উইকিপিডিয়া

Organ-on-a-chip

Symbiogenesis

Human skin color

Mutation

Sinopharm BIBP COVID-19 vaccine

Ati - Wikipédia

Proteins20

• Eyevensys is also investigating the potential to deliver genes that encode for other proteins with utility in a variety of ophthalmic diseases, such as glaucoma and retinitis pigmentosa. (medgadget.com)
• We do this with non-viral vector plasmids, which can encode a variety of therapeutic proteins with a low risk of immunogenicity, so it's less likely to generate an immune response. (medgadget.com)
• Since the 1990s, the development of anti-retroviral therapies means that viral proteins can be inhibited and viral load reduced to undetectable levels. (biologists.com)
• The proteins produced from these three genes are GTPases. (medlineplus.gov)
• A computer model created by researchers at Oregon Health and Science University suggested that variations in this gene from one person to the next could result in proteins that are better or worse at recognizing and flagging the coronavirus in the body. (medscape.com)
• For example, modifying a chemical reaction called methylation can change the function of a gene, causing it to increase or decrease production of certain proteins or to produce different kinds of proteins. (merckmanuals.com)
• To work out what these genes are doing the scientists needed to know where they are active, so they looked for RTL5/6 proteins, which are only produced when genes are switched on. (thetokyonews.net)
• Even now, some of its proviruses within the human genome still retain the ability to make viral proteins. (the-scientist.com)
• Furthermore, each tissue sample also contained genetic material from at least one provirus that could still produce viral proteins. (the-scientist.com)
• Importantly, it also means that HERV genes or proteins may no longer be good targets for drugs. (the-scientist.com)
• Synthetic vaccinology uses information from viral gene sequencing to create DNA and mRNA molecules encoding viral proteins. (nih.gov)
• In this strain, 1 putative cleavage site of the viral polyprotein responsible for processing of structural proteins was changed. (cdc.gov)
• The scope of this study encompasses an investigation of the markets cell and gene therapy tools such as GMP proteins, media, cell separation and activation reagents, viral and non-viral, cytokine release syndrome monitoring products, GMP antibodies, leukapheresis instrumentation, immunoassays (multiplex and singleplex) and bioreactors. (medgadget.com)
• Proteins coded by the retroviral gag gene. (clustermed.info)
• Cytoplasmic domains of cellular and viral integral membrane proteins substitute for the cytoplasmic domain of the vesicular stomatitis virus glycoprotein in transport to the plasma membrane. (rupress.org)
• Biochemical analyses and immunofluorescence microscopy demonstrated that these hybrid genes were correctly expressed in eukaryotic cells and that the hybrid proteins were transported to the plasma membrane. (rupress.org)
• The method of constructing precise hybrid proteins described here will be useful in defining functions of specific domains of viral and cellular integral membrane proteins. (rupress.org)
• May recruit ribosome to viral RNA thereby promoting viral proteins translation (By similarity). (icr.ac.uk)
• The largest, ORF-1, codes for the nonstructural proteins responsible for viral replication. (medscape.com)
• COVID-19 can be diagnosed by detection of RNA gene targets (e.g., spike protein (S), an envelope protein (E), nucleocapsid protein (N), RNA-dependent RNA polymerase enzyme, and ORF1 gene) (4-6) either by nucleic acid amplification testing or detection of virus-specific proteins by antigen testing (7,8). (who.int)

Genome16

• This work adapted clinically relevant high-capacity adenoviral vectors devoid of all viral genes for the delivery of the CRISPR/Cas9 machinery using a single viral vector to enable fast transfer of the Cas9 gene and gRNA expression units into the HCAdV genome. (semanticscholar.org)
• Here we present the X-ray crystal structure of 2A and show that it selectively binds to a pseudoknot-like conformation of the PRF stimulatory RNA element in the viral genome. (cam.ac.uk)
• Indeed, genes within a genome have not all followed the same evolutionary path due to events such as incomplete lineage sorting, horizontal gene transfer, gene duplication and deletion, or recombination. (upv.es)
• To illustrate the applicability of the method, we have chosen two viral datasets, namely papillomaviruses and Turnip mosaic virus (TuMV) isolates, largely dissimilar in genome, evolutionary distance and biology. (upv.es)
• RORC2 inhibition did not decrease the reverse transcription of the viral RNA, nuclear entry nor integration of viral DNA into the genome. (biologists.com)
• RORC2 binds specifically in a region of the viral genome that is characterized to bind other retinoic acid receptors. (biologists.com)
• As researchers begin to comb the human genome - that's all your estimated 25,000 genes - for genes that might play a role in COVID-19 infection, they have a few suspects in mind. (medscape.com)
• Viral products made during productive infection, perhaps in combination with signals present in an intact viral genome, possess the ability to override the signals recognized when segments of the viral genome become integrated. (princeton.edu)
• The researchers were convinced that the genes must be doing something important, as despite coming from viruses, these inherited viral genes have been preserved in the mammalian genome for at least the last 120 million years. (thetokyonews.net)
• According to Dr Ishino, "virus-acquired genes are essential parts of our genome, playing various - but essential - roles in mammalian and human development. (thetokyonews.net)
• Viral remnants make up 8 percent of the human genome, and a new study finds that these sequences are still active in healthy people. (the-scientist.com)
• The Alliance for Regenerative Medicine's (ARM) Gene Editing Task Force on Tuesday released a set of principles for human genome editing endorsed by thirteen of its members who are involved in the development of gene therapies or gene-editing technology. (genetherapynet.com)
• Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
• Adenovirus DNA : the viral genome and its expression / edited by Walter Doerfler. (who.int)
• Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. (icr.ac.uk)
• In this project I performed experimental evolution of a small RNA virus to asses the stability of changes in its genome architecture (relocation, duplication and insertion of foreign genes). (weebly.com)

Viruses23

• A jumping gene first identified in a cabbage-eating moth may one day provide a safer, target-specific alternative to viruses for gene therapy, researchers say. (technologynetworks.com)
• Scientists have used viruses as a gene delivery mechanism for 20 years because of their adeptness at getting inside cells and inserting themselves in DNA. (technologynetworks.com)
• Thus, this anti-viral mechanism has evolved to work against many different viruses. (benaroyaresearch.org)
• Viruses can be used as vectors (vehicles) for gene delivery to treat several congenital and acquired diseases in humans (Figure 1). (mpg.de)
• For example, sometimes the genes of human and plant viruses are interchangeable, while on other occasions inserting plant viral fragments as transgenes has caused the genetically altered plant to become susceptible to an animal virus (Dasgupta et al. (greenmedinfo.com)
• Influenza A(H3N2) virus HA, neuraminidase (NA), and matrix (M) gene sequences from 5 specimens collected from patients in November 2014 were directly sequenced from the specimens, and corresponding first-passage viruses were isolated in MDCK cells by using previously described primers ( 6 , 7 ). (cdc.gov)
• those changes are characteristic of HA gene clade 3C.2a viruses ( 4 ). (cdc.gov)
• An H3N2 virus isolated in February 2014 in Gainesville, designated A/GNVL/01/2014 (H3N2), was from a different HA gene clade ( Table 1 ), suggesting that the clade 3C.2a viruses were introduced locally later. (cdc.gov)
• Two independent teams of scientists from the University of Utah and the University of Massachusetts Medical School have discovered that a gene crucial for learning, called Arc, can send its genetic material from one neuron to another by employing a strategy commonly used by viruses. (evesdrift.com)
• In addition, Dr. Shepherd believes it may be possible to use Arc capsids for genetic engineering and gene therapy, which currently use viruses to introduce new genetic instructions into cells. (evesdrift.com)
• The idea that viruses have had such a positive impact on our lives may be surprising, but examples like Rtl5 and Rtl6 demonstrate that viral invaders can, in the long run, benefit their host. (thetokyonews.net)
• Viruses have a natural ability to deliver genetic material into cells, and therefore some gene therapy products are derived from viruses. (fda.gov)
• Once viruses have been modified to remove their ability to cause infectious disease, these modified viruses can be used as vectors (vehicles) to carry therapeutic genes into human cells. (fda.gov)
• A study in the journal Science shows that staph bacteria are able to use viruses to insert their dangerous genes into the genomes of other species of bacteria. (scientificamerican.com)
• Because it often uses repurposed viruses to deliver therapeutic genes, gene therapy has been caught in a vicious cycle for nearly two decades owing to immune response, insertional mutagenesis, viral tropism, off-target activity, unwanted clinical outcomes (ranging from illness to death of participants in clinical trials), and patchy regulations (23, 28-31). (swfinstitute.org)
• Historically, vaccines against viral diseases have used live-attenuated (weakened) viruses or inactivated whole viruses to induce protective immune responses. (nih.gov)
• There is little information on their evolution in Jordan so this study investigated the genetic and antigenic variability of A(H1N1)pdm09 and H3N2 viruses in Jordan by performing phylogenetic and genetic analyses of the HA and NA genes of A(H1N1)pdm09 and H3N2 viruses between 2011 and 2013. (who.int)
• The full HA and NA genes of 16 H1N1-positive samples obtained in our study and 21 published HA sequences and 20 published NA sequences from Jordanian viruses that were available on online gene databases were analysed. (who.int)
• Humans and viruses have co-evolved for millennia resulting in genetic polymorphisms that affect response to viral infection. (medrxiv.org)
• We conducted a comprehensive study in the UK biobank linking germline genetic variation and gene expression with 28 antigens for 16 viruses in 7924 subjects. (medrxiv.org)
• Giant viruses have blurred the classic definition of viruses, as their recent discovery eliminated the separation of cellular and viral genomes by size. (weebly.com)
• Scientists have known for about a decade that some more aggressive types of cancer express high levels of interferon-stimulated genes (ISGs), an action usually triggered by the presence of viruses. (sciencealert.com)
• While mutant viruses lacking the Us9 gene have no obvious growth or plaque size defects in tissue culture, these mutants are defective for anterograde directional spread in a subset of retinal ganglion neurons that make up visual circuitry after infection of the rat eye. (vetres.org)

Infection26

• Moreover, many patients are precluded from treatment because they have developed neutralizing antibodies (NAbs) for AAV, either through natural infection or from a previous gene therapy treatment. (bioprocessintl.com)
• Researchers used a transposon-mediated gene-activation screen to search for new genes that can prevent infection by Ebola virus. (benaroyaresearch.org)
• By testing well-characterised RORC2 inhibitors created by the pharmaceutical company GlaxoSmithKline, the authors identify RORC2 as a host co-factor for HIV-1 infection that activates HIV-1 gene expression. (biologists.com)
• Such a factor may integrate the organismal response to virus infection, encompassing both immune and nonimmune anti-viral responses. (uab.edu)
• COVID-19 has highlighted a need for novel diagnostic approaches to indicate likelihood of viral infection in a pandemic setting. (jenner.ac.uk)
• We aimed to derive and validate a blood transcriptional signature to detect viral infections, including COVID-19, among adults with suspected infection who presented to the emergency department.MethodsIndividuals (aged ≥18 years) presenting with suspected infection to an emergency department at a major teaching hospital in the UK were prospectively recruited as part of the Bioresource in Adult Infectious Diseases (BioAID) discovery cohort. (jenner.ac.uk)
• Whole-blood RNA sequencing was done on samples from participants with subsequently confirmed viral, bacterial, or no infection diagnoses. (jenner.ac.uk)
• This gene, also involved in flu infection, helps create a protein that the coronavirus uses to get inside human cells. (medscape.com)
• When Goldstein and his colleagues looked for genes that play a part in hepatitis C infection years ago, he says, "Everyone had their favorite candidate genes they were looking at. (medscape.com)
• It turned out, a mutation in that unsuspected gene raises your risk for infection and tells whether the usual treatment at the time would work. (medscape.com)
• They found that microglia containing RTL6 protein responded to the bacteria-like mimic, whereas the microglia with RTL5 reacted to the simulated viral infection. (thetokyonews.net)
• In addition, when the researchers removed the Rtl6 gene, they found that the mice could not eliminate the fake bacterial infections, while the mice without Rtl5 could not clear the viral mimics, meaning that together Rtl5 and Rtl6 protect the brain against two of the most common types of infection. (thetokyonews.net)
• These results provide the first example of viral-derived genes that have been re-purposed to protect mammalian brains against infection. (thetokyonews.net)
• Finally, transfection of cells with miRNA4276 inhibitor and subsequent infection with influenza A led to reduced influenza viral copy number. (cdc.gov)
• Viral Infection-Induced Differential Expression of LncRNAs Associated with Collagen in Mouse Placentas and Amniotic Sacs. (nih.gov)
• Two years later across the world in China, two girls, also as embryos, had their genes edited using CRISPR to make them (theoretically) resistant to HIV infection. (genetherapynet.com)
• Mechanistic studies were undertaken to assess viability, replication efficacy, viral infection enhancement and cell death pathway induction in a selected panel of drugs. (eur.nl)
• The viral sensitizers interact with infection, replication and cell death pathways to enhance efficacy of the OV. (eur.nl)
• Control measures for outbreaks of viral gastroenteritis should focus on the removal of an ongoing common source of infection (e.g., an ill food handler or the contamination of a water supply) and on the interruption of person-to-person transmission that can perpetuate an outbreak in a population after the common source has been removed. (cdc.gov)
• The viral S gene is important as it codes for the Spike protein which is the molecule that makes contact with, and allows entry of the virus into susceptible host cells, causing infection. (who.int)
• To date, WHO has designated 4 SARS CoV-2 genotypes that meet the working definition of a VOC: i) contain mutations divergent from the Wuhan strain and ii) that the mutations impact one or more of the aspects of viral infection that have public health significance. (who.int)
• Our findings elucidate the genetic architecture of host response to viral infection, with potential implications for complex diseases and COVID-19. (medrxiv.org)
• For example, treatment of hepatitis C, a common viral infection and a cause of liver cirrhosis, with pegylated interferon plus ribavirin is complicated by hemolytic anemia induced by ribavirin. (cdc.gov)
• The U.S. National Institute of Allergy and Infectious Diseases (NIAID), which is part of the National Institutes of Health (NIH), is leading the Antiviral Program for Pandemics (APP ) to stimulate discovery and development of oral or intranasal antiviral medicines that could reduce viral burden early in the infection. (faegredrinker.com)
• The conundrum was that in most cases, there was no viral infection in these tumours," says the lead researcher Andy J. Minn from University of Pennsylvania. (sciencealert.com)
• Detection of viral-specific antibodies enables a more accurate and precise diagnosis and the progression of infection and treatment responses against COVID-19 to be monitored (11,12). (who.int)

Therapy74

• Eyevensys , a clinical-stage biotechnology company based in France, has developed a method to perform non-viral gene therapy in the eye, with the aim of treating ocular diseases. (medgadget.com)
• On October 10th, Eyevensys presented results from its phase I/II clinical study of its gene therapy in non-infectious uveitis patients at the Annual Ophthalmology Innovation Summit in San Francisco . (medgadget.com)
• The trial included patients with late-stage non-infectious uveitis, and those treated using the gene therapy showed improvements in vision and macular edema up to 6 months after just one treatment. (medgadget.com)
• Please give us some background on the Eyevensys non-viral gene therapy system. (medgadget.com)
• What is it like for patients to undergo gene therapy using the system? (medgadget.com)
• Efficient gene transfer with extended gene expression is essential for successful treatment of skin diseases using gene therapy. (duke.edu)
• This article explores potential advantages of annelloviruses as gene therapy vectors. (bioprocessintl.com)
• It can take years or even decades to bring a novel gene therapy from drug discovery through regulatory approval. (bioprocessintl.com)
• They compared the ability of the four best-characterized jumping genes, or transposons, to insert themselves into a cell's DNA and produce a desired change, such as making the cell resistant to damage from radiation therapy. (technologynetworks.com)
• Gene therapy trials have been halted because of major complications, including deaths. (technologynetworks.com)
• A unique, improved viral vector for use in treating sickle cell disease with gene therapy has recently been created by researchers from the National Institutes of Health (NIH). (docwirenews.com)
• The use of this new technique could potentially make gene therapy more effective and prevalent in treating sickle cell disease, which affects roughly 100,000 people in the US and millions globally. (docwirenews.com)
• Our new vector is an important breakthrough in the field of gene therapy for sickle cell disease," said senior author John Tisdale, MD, chief of the Cellular and Molecular Therapeutic Branch at the National Heart, Lung, and Blood Institute (NHLBI). (docwirenews.com)
• In gene therapy, the viral vector serves as a delivery vehicle that leverages the virus's innate ability to infiltrate host cells and administer genetic material. (docwirenews.com)
• Gene therapy for sickle cell involves the modification of bone marrow hematopoietic stem cells. (docwirenews.com)
• Gene therapy studies that incorporate these reverse-oriented vectors for sickle cell disease and beta-thalassemia , a similar inherited blood disease, have been encouraging thus far. (docwirenews.com)
• The universal purification method was reported for several variants of adeno-associated virus (AAV), widely used in human gene therapy. (mpg.de)
• ContiVir , a spin-off project at the Max Planck Institute for Dynamics of Complex Technical Systems Magdeburg, has published results in the scientific journal Human Gene Therapy detailing the purification of Adeno-associated virus (AAV) using membrane-based steric exclusion chromatography (SXC), a technology developed by Dr.-Ing. (mpg.de)
• Dr. Julian Lopez, responsible for Business Development of the ContiVir project, commented: "Our SXC technology has many of the attributes that the gene therapy industry is after when looking at new ways to truly tackle current and future challenges in manufacturing of virus particles, such as higher speed, increased yield, and plug-and-play & single-use operation. (mpg.de)
• In some instances, we were able to recover with the smallest SXC device an amount of AAV particles equivalent to 20 retinal gene therapy treatments in less than one hour from a cell fraction that is commonly discarded in many laboratories because they lack the capacity to process larger volumes. (mpg.de)
• SXC is crucial for the availability of universal and scalable purification tools and their successful application in human gene therapy. (mpg.de)
• The smallest device can recover an amount of AAV particles equivalent to about 20 retinal gene therapy treatments. (mpg.de)
• Gene therapy is the approach that designed to introduce a genetic material (DNA or RNA) as a medicament to the patient. (foxchase.org)
• Thus, the administration of RNA virus vectors containing these genes can either be part of cancer therapy, or the treatment of a viral influenzainfection. (foxchase.org)
• IMPORTANCE OF THE FIELD: Gene therapy is regarded as one of the most promising therapeutic approaches, as it has the potential to treat disorders by correcting malformations at the nucleic acids. (ox.ac.uk)
• Highlighting requirements of the 'Laboratory Evaluation' stage in order for successful cell and gene therapy translation. (ucl.ac.uk)
• In order to develop a research grade cell and gene therapy vector, there are a number of factors to consider in order to ensure the vector is stable, sufficient and optimised for preclinical evaluation. (ucl.ac.uk)
• Effective cell therapy eradication via suicide gene activation in vitro? (ucl.ac.uk)
• The Translational Research Group (TRG) within the Translational Research Office, have extensive knowledge of the cell and gene therapy translational pathway and are experienced on working on projects in this field. (ucl.ac.uk)
• Although gene therapy is defined as any treatment that changes gene function, it is often thought of as the insertion of normal genes into the cells of a person who lacks such normal genes because of a specific genetic disorder. (merckmanuals.com)
• This technique is called gene insertion therapy or insertion gene therapy. (merckmanuals.com)
• Currently, such gene insertion therapy is most likely to be effective in the prevention or cure of single-gene defects, such as cystic fibrosis. (merckmanuals.com)
• A different method of gene therapy uses antisense technology. (merckmanuals.com)
• However, it seems to have the potential to be more effective and safer than gene insertion therapy. (merckmanuals.com)
• Another approach to gene therapy is to increase or decrease the activities of certain genes by modifying chemical reactions in the cell that control gene expression. (merckmanuals.com)
• Gene therapy is also being studied experimentally in transplantation surgery. (merckmanuals.com)
• We support your cell and gene product, viral vaccine or oncolytic virus therapy development from pre-clinical to cGMP manufacturing. (exothera.world)
• The Director, Business Development, Viral Gene Therapy (internally known as Commercial Development Manager) sells FUJIFILM Diosynth Biotechnologies. (biospace.com)
• Gene therapy covers a broad spectrum of applications, from gene replacement and knockdown for genetic or acquired diseases such as cancer, to vaccination, each with different requirements for gene delivery. (ox.ac.uk)
• Viral vectors and synthetic liposomes have emerged as the vehicles of choice for many applications today, but both have limitations and risks, including complexity of production, limited packaging capacity, and unfavorable immunological features, which restrict gene therapy applications and hold back the potential for preventive gene therapy. (ox.ac.uk)
• Exploiting the natural properties of these biological entities for specific gene delivery applications will expand the repertoire of gene therapy vectors available for clinical use. (ox.ac.uk)
• Here, we review the prospects for nonviral biological delivery vehicles as gene therapy agents with focus on their unique evolved biological properties and respective limitations and potential applications. (ox.ac.uk)
• The potential of these nonviral biological entities to act as clinical gene therapy delivery vehicles has already been shown in clinical trials using bacteria-mediated gene transfer and with sufficient development, these entities will complement the established delivery techniques for gene therapy applications. (ox.ac.uk)
• What is Gene Therapy? (fda.gov)
• Human gene therapy seeks to modify or manipulate the expression of a gene or to alter the biological properties of living cells for therapeutic use 1 . (fda.gov)
• Gene therapy is a technique that modifies a person's genes to treat or cure disease. (fda.gov)
• Gene therapy products are being studied to treat diseases including cancer, genetic diseases, and infectious diseases. (fda.gov)
• Gene therapy products are biological products regulated by the FDA's Center for Biologics Evaluation and Research (CBER). (fda.gov)
• Marketing a gene therapy product requires submission and approval of a biologics license application (BLA). (fda.gov)
• Improving Efficiency of Non-viral Gene Therapy by InceasingDelivery of Transcriptionally active DNA to the Nucleus. (birmingham.ac.uk)
• Novartis dismissed the top two scientists at its gene therapy division shortly after CEO Vas Narasimhan learned of internal data falsification that has since snowballed into a damaging scandal, a person familiar with the situation said Wednesday. (genetherapynet.com)
• The person familiar with the situation, who spoke on condition of anonymity, confirmed that the departure of the Kaspars, who are brothers, was connected to the disclosure of data manipulation related to the gene therapy Zolgensma. (genetherapynet.com)
• The progression of gene therapy through clinical trials is contributing to millions of lives extended as well as more patients going into remission. (genetherapynet.com)
• The success gene therapy is playing towards these common cancers are making waves in the rare space as well. (genetherapynet.com)
• Among these are gene therapy products. (who.int)
• In January 2005, an informal consultation was convened by WHO to consider use of INNs for gene therapy products and to agree the outline of a possible nomenclature system. (who.int)
• The meeting involved participation of experts in nomenclature as well as those in biologicals, biotechnology and gene therapy. (who.int)
• It was not the intention, at this stage, to develop a complete and detailed INN system for gene therapy medicinal products but to establish a basis for further discussion and activities, with an emphasis on wider consultation. (who.int)
• Gene therapy involves the delivery of exogenous DNA into the target cells in order to produce therapeutic protein or to correct a genetic defect. (helsinki.fi)
• Gene therapy is a potential treatment for age related macular degeneration (AMD). (helsinki.fi)
• The mRNA vaccinations are a form of gene therapy, according to its definition in many parts of the world, including Europe. (swfinstitute.org)
• According to the Mayo Clinic website, "Gene therapy involves altering the genes inside your body's cells in an effort to treat or stop disease. (swfinstitute.org)
• Gene therapy replaces a faulty gene or adds a new gene in an attempt to cure disease or improve your body's ability to fight disease. (swfinstitute.org)
• Gene therapy holds promise for treating a wide range of diseases, such as cancer, cystic fibrosis, heart disease, diabetes, hemophilia and AIDS. (swfinstitute.org)
• The American Society of Gene Therapy has taken lead in fixing this fragmented funding method by making many recommendations including the elimination of redundant regulatory processes and establishment of the National Gene Vector Laboratories (NGVL) to review vector production and toxicology. (swfinstitute.org)
• Examples include live viral vaccines, stem cells, and gene therapy viral vectors. (bioreliance.com)
• Gene and cell therapy are emerging as important tools to treat human health. (medgadget.com)
• The promise of gene therapy using technologies such as CRISPR is starting to be realized in clinical trials, and markets are scaling up to treat other diseases as well, particularly rare gene- based diseases. (medgadget.com)
• Our GMP plasmid DNA may also be used to develop novel DNA or mRNA vaccines and non-viral gene therapy applications. (biovian.com)
• The Horae Gene Therapy Center (HGTC) of UMMS is a critical component of the UMass Advanced Therapeutics Cluster. (aavaatx.com)
• The GTC is a world leader in AAV discovery, vector development, production, quality control testing and applications for pre-clinical gene therapy studies. (aavaatx.com)
• The Horae Viral Vector Core (HVVC) is an indispensable unit of HGTC, serving to elevate our reputation among academic and industrial users throughout the world, driving gene therapy research at UMMS, and serving many gene therapy researchers around the world. (aavaatx.com)
• Chemoinducible gene therapy: a strategy to enhance doxorubicin antitumor activity. (uchicago.edu)
• Transcriptional control of viral gene therapy by cisplatin. (uchicago.edu)

Protein21

• A topical review of recent advances to in vivo delivery of the CRISPR/Cas9 system using various packaging formats, including viral, mRNA, plasmid, and protein-based approaches, focusing on the challenges to efficacy for various delivery formats. (semanticscholar.org)
• The data we just presented are from part 1 of our phase I/II clinical safety study for EYS606, which is a non-viral vector that encodes an anti-TNFα protein. (medgadget.com)
• Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch. (cam.ac.uk)
• The HRAS gene provides instructions for making a protein called H-Ras that is involved primarily in regulating cell division. (medlineplus.gov)
• The HRAS gene mutations that cause Costello syndrome lead to the production of an H-Ras protein that is abnormally turned on (active) in cells throughout the body. (medlineplus.gov)
• The somatic HRAS gene mutations involved in epidermal nevi, change single amino acids in the H-Ras protein. (medlineplus.gov)
• Genes and Chromosomes Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body. (merckmanuals.com)
• To their surprise, when the cells made the Arc protein, it clumped together into a form that resembled a viral capsid, the shell that contains a virus' genetic information. (evesdrift.com)
• Both drugs are absorbed well orally, block the viral M2 protein ion channel, and inhibit the uncoating of the virus. (medscape.com)
• Pregnancy in all mammals is dependent on the virus-derived protein coded in this gene. (the-scientist.com)
• IMSEAR at SEARO: Generation of transgenic mesenchymal stem cells expressing green fluorescent protein as reporter gene using no viral vector in caprine. (who.int)
• While there are many ways to label and track cells each with strengths and weakness, the green fluorescent protein (GFP) is a reporter gene commonly employed. (who.int)
• Zolgensma is designed to address the genetic root cause of SMA by providing a functional copy of the human SMN gene to halt disease progression through sustained SMN protein expression with a single, one-time intravenous (IV) infusion. (genetherapynet.com)
• Some mutations in the S gene may lead to changes in the spike protein which result in inhibition of contact and entry of the virus into human cells, however in the case of the VOC, they contain mutations in the S gene that enhance the process of contact and entry into human cells, increasing transmissibility of the virus. (who.int)
• The S gene is one of the structural genes of the virus which encodes for a protein that sits on the surface of the SARS CoV-2 virus. (who.int)
• The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. (clustermed.info)
• Adenoviruses, which exist in the wild in humans and typically cause mild infections such as the common cold, have been genetically engineered to express viral antigens found in SARS-CoV-2, usually those of the infamous spike protein that the coronavirus uses to break into human cells. (allianceforscience.org)
• Viral protein 1 amino acids were 67.9%-77.7% identical and had a distance of at least 39.4% from known cardioviruses. (cdc.gov)
• Protein p48 may play a role in viral replication by interacting with host VAPA, a vesicle-associated membrane protein that plays a role in SNARE-mediated vesicle fusion. (icr.ac.uk)
• By fusing the jellyfish enhanced green fluorescent protein reporter molecule (EGFP) to the carboxy-terminus of Us9, we demonstrated that Us9 not only is capable of targeting a Us9-EGFP fusion protein to the Golgi compartment, it also is able to direct efficient incorporation of such chimeric molecules into infectious viral particles. (vetres.org)
• and viral protein 1 (VP1) and viral protein 2 (VP2) genes, complete cds. (cdc.gov)

Expression18

• A set of exciting new strategies that aim at further increasing the therapeutic index of CRISPR technologies, by reducing the size of Cas9 expression cassettes and thus enhancing their compatibility with viral gene delivery vectors are highlighted. (semanticscholar.org)
• Quantitative analysis of reporter lacZ gene expression showed that the viral vector-mediated gene transfers were superior to gene-gun delivery of plasmid DNA. (duke.edu)
• We found that aDMA-modified EBNA 2 preferentially bound promoters to regulate gene expression, implicating this modification as a key regulator of EBNA 2 activity. (sussex.ac.uk)
• This new screening strategy-that serves as a blueprint for uncovering resistance mechanism against other dangerous pathogens- found that the gene MHC class II transactivator (CIITA) induces resistance in human cell lines by activating the expression of a second gene, CD74. (benaroyaresearch.org)
• Since RORC2 is a transcription factor, it can activate viral gene expression by directly binding to the integrated viral DNA. (biologists.com)
• CTL-mediated selection for loss of expression of Mta by H-2-heterozygous SV40-transformed mouse fibroblasts (line 24SV) produced an unusual phenotypic class of maternally transmitted Ag negative mutants defective in both MHC expression and in anti-viral activity. (uab.edu)
• Efficient gene transfer by electroporation of chick embryos in ovo has allowed the development of new approaches to the analysis of gene regulation, function and expression, creating an exciting opportunity to build upon the classical manipulative advantages of the chick embryonic system. (nature.com)
• Figure 1: Electroporation-mediated gene expression in cultured chick and mouse embryos. (nature.com)
• Petropoulos, C. & Hughes, S. Replication-competent retrovirus vectors for the transfer and expression of gene cassettes in avian cells. (nature.com)
• Comparison of three non-viral transfection methods for foreign gene expression in early chicken embryos in ovo . (nature.com)
• This chapter discusses the organization and expression of viral genes in adenovirus-transformed cells. (princeton.edu)
• Flint, SJ 1982, ' Organization and expression of viral genes in adenovirus-transformed cells ', International Review of Cytology , vol. 76, no. (princeton.edu)
• These "antibody-selectable" vectors are a promising alternative to conventional expression systems for using antibodies to clone genes, though the ability to isolate rare clones from actual libraries remains to be demonstrated. (nih.gov)
• Further experiments demonstrated that resveratrol had little effect on VACV early gene expression, while it suppressed VACV DNA synthesis, and subsequently post-replicative gene expression. (researchgate.net)
• We also link viral response genes with complex diseases, such as C4A expression in varicella zoster virus and schizophrenia. (medrxiv.org)
• Quantitative polymerase-chain-reaction (qPCR) was used to investigate gene expression of ICAM-1 and MUC5 A/C. The viral/bacterial load was investigated in lung homogenate or BAL fluid. (uni-wuerzburg.de)
• Expression in Escherichia coli of cDNA fragments encoding the gene for the host-protective antigen of infectious bursal disease virus. (nchu.edu.tw)
• Expression of p63 is almost exclusively restricted to epithelial cells, mutations in this gene are infrequent, and its expression is increased in a variety of solid tumors, particularly those of the head and neck area 12,13 . (bvsalud.org)

Therapies13

• Read the articles below to learn more about increasing the scalability and efficiency of existing platform processes, making the most of vector-production facilities, minimizing immunogenicity risks with traditional vectors, and even developing alternative viral systems for delivery of gene therapies. (bioprocessintl.com)
• Immunogenicity remains a primary concern for developers of gene therapies based on adenoassociated virus (AAV) vectors. (bioprocessintl.com)
• The writers of this article show why addressing both preexisting and de novo immunogenicity hurdles will be critical to unlocking the potential of gene therapies. (bioprocessintl.com)
• They review current strategies for mitigating immune responses, highlighting recent developments involving coadministration of gene therapies and targeted immunosuppressive agents. (bioprocessintl.com)
• Published today in Science , these new findings provide a better understanding of cellular mechanisms involved in viral resistance that can inform future treatments and therapies for viral infectious diseases. (benaroyaresearch.org)
• The research illuminates a completely new role for the two genes identified and a unique approach to inhibiting virus fusion and entry into human cells-getting us one step closer to the next generation of antiviral therapies. (benaroyaresearch.org)
• Our work demonstrates one way in which cells can be modified to do this, and we hope that our insights will open up new avenues for scientists developing therapies and interventions to treat viral infectious diseases that impact millions of lives around the world. (benaroyaresearch.org)
• Although the approval of the first cell and gene therapies has occurred only in recent years, the industrial manufacturing for these products still relies on technologies that are more than 30 years old and often display low product yields and even the risk of batch failures. (mpg.de)
• With a growing number of gene therapies approved in the next years, a still increasing world population, and the emergence of serious public health threats such as COVID-19 that highly disrupt the biopharmaceutical supply chain, experts agree that incremental changes in production capacity with current technologies will not be enough to cope with the huge demand for virus manufacturing. (mpg.de)
• But the typical method for delivering gene therapies to specific tissues in the body can be complicated and may cause troubling side effects. (genetherapynet.com)
• As the vaccinations begin to spread among the world population, the growth of other gene therapies as a type of vaccination could increase. (swfinstitute.org)
• Gene therapies remain under strict regulation and few gene therapeutics have been approved by health authorities because of safety concerns. (swfinstitute.org)
• One of our main focus areas is viral vector manufacturing for those gene therapies, where plasmid DNA constructs serve as key raw material. (biovian.com)

MRNA1

• The viral C promoter (Cp), regulated by EBNA 2, drives transcription of an ~120 kb pre-mRNA that is differentially spliced to generate messages encoding all the other EBNAs required for immortalisation. (sussex.ac.uk)

Therapeutic8

• The system uses ocular electrotransfection to deliver therapeutic genes into the eye. (medgadget.com)
• Adenoassociated virus (AAV) and lentivirus (LV) remain the biopharmaceutical industry's preferred systems for in vivo and ex vivo delivery of therapeutic genes, respectively. (bioprocessintl.com)
• If we want to add a therapeutic gene, we can put it within the transposon and use it to deliver the gene into the cell," says Dr. Joseph M. Kaminski, radiation oncologist at the Medical College of Georgia Cancer Center and a corresponding author on research published Sept. 25 in the online Proceedings of the National Academy of Sciences Early Edition. (technologynetworks.com)
• The vector is modified to carry a beneficial gene that will induce therapeutic effects in the patient, often by counteracting a genetic mutation. (docwirenews.com)
• The NIH team found that their vector delivered a viral load of up to six times more therapeutic beta-globin genes than the traditional vector and had four to ten times higher transduction efficiency (ability to incorporate therapeutic genes into repopulating bone marrow cells). (docwirenews.com)
• Consequently, the approach formed on viral-based targeted gene delivery of DAI, RIPK3 and MLKL, can accomplish the therapeutic response and reduce the volume of tumor or virally-infected cells. (foxchase.org)
• Circular DNA molecules can be genetically engineered to carry therapeutic genes into human cells. (fda.gov)
• Bacteria can be modified to prevent them from causing infectious disease and then used as vectors (vehicles) to carry therapeutic genes into human tissues. (fda.gov)

Replication3

• M2 ion channel blockade inhibits HA a cleavage beta block RNA encoding, which reduces early viral replication. (medscape.com)
• As a result, these drugs did not prevent viral replication in infected persons and, moreover, were potentially toxic. (faegredrinker.com)
• Corticosteroids have no impact on cold symptoms and may actually increase viral replication. (medscape.com)

Infections8

• We really don't understand the cellular mechanisms that block viral infections which has limited our ability to effectively respond to pandemics, including this year's coronavirus," said Lynda M. Stuart, MD, PhD, Deputy Director, Bill & Melinda Gates Foundation, BRI Affiliate Investigator and co-author on the study. (benaroyaresearch.org)
• Discovery and validation of a three-gene signature to distinguish COVID-19 and other viral infections in emergency infectious disease presentations: a case-control and observational cohort study. (jenner.ac.uk)
• We assessed signature performance by calculating the area under receiver operating characteristic curves (AUROCs), sensitivities, and specificities.FindingsA three-gene transcript signature, comprising HERC6, IGF1R, and NAGK, was derived from the discovery cohort of 56 participants with bacterial infections and 27 with viral infections. (jenner.ac.uk)
• In the validation cohort of 200 participants, the signature differentiated bacterial from viral infections with an AUROC of 0·976 (95% CI 0·919-1·000), sensitivity of 97·3% (85·8-99·9), and specificity of 100% (63·1-100). (jenner.ac.uk)
• The signature achieved higher net benefit in decision curve analysis than either CRP or leukocyte count for discriminating viral infections from all other infections. (jenner.ac.uk)
• Dysregulation of these processes has major pathological consequence and studies have shown that cytochrome oxidases are altered during viral infections. (cdc.gov)
• New tools for editing genetic code offer hope for new treatments for inherited diseases, some cancers, and even stubborn viral infections. (genetherapynet.com)
• The main objective of this study was to measure oral HPV viral load for the subset of oral rinse samples (ORS) that were positive for high-risk or probably high-risk HPV types from the NHANES 2009-2010 study entitled Prevalence of Oral HPV Infections in the United States Population. (cdc.gov)

Mutations16

• A major segment of the neurofibromatosis type 1 gene: cDNA sequence, genomic structure, and point mutations. (nih.gov)
• This system, however, is not able to correct or edit gene mutations. (medgadget.com)
• At least 15 mutations in the HRAS gene have been identified in people with Costello syndrome, a rare condition that affects many parts of the body and increases the risk of developing cancerous and noncancerous tumors. (medlineplus.gov)
• Researchers are uncertain how mutations in the HRAS gene cause the other features of Costello syndrome (such as intellectual disability, distinctive facial features, and heart problems), but many of the signs and symptoms probably result from cell overgrowth and abnormal cell division. (medlineplus.gov)
• Mutations in the HRAS gene are involved in the development of abnormal, noncancerous patches of skin called epidermal nevi (singular: nevus). (medlineplus.gov)
• HRAS gene mutations have been found in a majority of people with a certain type of epidermal nevus called a nevus sebaceous. (medlineplus.gov)
• HRAS gene mutations are less commonly found in keratinocytic epidermal nevi, a type of epidermal nevus that involves a particular type of epidermal cell called a keratinocyte. (medlineplus.gov)
• Epidermal nevi are caused by gene mutations that are acquired during the early stages of development before birth. (medlineplus.gov)
• Somatic HRAS gene mutations are also involved in development of Schimmelpenning syndrome, which is a type of epidermal nevus syndrome. (medlineplus.gov)
• AveXis, a Novartis company, today announced the US Food and Drug Administration (FDA) has approved Zolgensma® (onasemnogene abeparvovec-xioi) for the treatment of pediatric patients less than 2 years of age with spinal muscular atrophy (SMA) with bi-allelic mutations in the survival motor neuron 1 (SMN1) gene. (genetherapynet.com)
• Peu de renseignements sont disponibles sur les mutations des virus saisonniers de la grippe A(H1N1)pdm09 et H3N2 en Jordanie. (who.int)
• Les mutations individuelles sont décrites en détail. (who.int)
• The unique synonymous mutations detected in the E and Non-structural 2a genes of Usutu-BONN strains may suggest an adaptive evolution. (cdc.gov)
• Currently, manufacturers are focusing on and targeting assays to mutations in the S gene. (who.int)
• A common form of MODY is due to mutations in the HNF1A gene. (cdc.gov)
• Although mutations in the gene underlying MODY were identified before the GWAS era, they could have important implications for patients and their relatives, as many patients with HNF1A‑MODY are better managed with sulphonylureas than with metformin or insulin. (cdc.gov)

Genomes2

• R emnants of ancient viral pandemics in the form of viral DNA sequences embedded in our genomes are still active in healthy people, according to new research my colleagues and I recently published. (the-scientist.com)
• The most famous HERV embedded in human and animal genomes, syncytin , is a gene derived from an ancient retrovirus that plays an important role in the formation of the placenta. (the-scientist.com)

Adeno-associated viral1

• Effect of serotype 5 adenoviral and serotype 2 adeno- associated viral vector-mediated gene transfer to salivary glands on the composition of saliva. (bvsalud.org)

Research grade viral vectors2

• They are best placed to advise on considerations for research grade viral vectors and laboratory evaluation. (ucl.ac.uk)
• The Vector Core has grown into a world class supplier of high-quality research grade viral vectors shipping to over 80 research entities worldwide with over $10M in revenue accrued for the FY2014-2019 period. (aavaatx.com)

Phylogenetic3

• Results: The goal of the approach here proposed is to exploit this conflicting information: we have compiled eleven variables describing phylogenetic relationships and evolutionary pressures and submitted them to dimensionality reduction techniques to identify genes with similar evolutionary histories. (upv.es)
• Conclusions: The approach here presented allows turning phylogenetic inconsistencies into evolutionary information, detecting gene assemblies with similar histories, and could be a powerful tool for comparative pathogenomics. (upv.es)
• Salichos L, Rokas A. Inferring ancient divergences requires genes with strong phylogenetic signals. (upv.es)

Plasmid4

• High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. (nature.com)
• Passage 10 (P10) MSC cells were transfected using plasmid vector containing GFP as reporter gene with different concentrations of DNA and lipofectamine. (who.int)
• The carriers were complexed with episomal plasmid DNA or minicircles using secreted alkaline phosphatase (SEAP) gene as a marker gene. (helsinki.fi)
• According to the results, PBuA-PDMAEMA-polymers and DOTAP/DOPE/PS-liposomes complexed with episomal plasmid or minicircles are potential gene delivery agents for further studies in AMD. (helsinki.fi)

Goes viral3

• Published January 18, 2018 at 394×380 in Science News » Memory Gene Goes Viral . (brendansmile.org)
• Optical Illusion Goes Viral, so do YOU see two legs or. (webtopnews.com)
• Track Pants Optical Illusion Goes Viral on Social Media. (webtopnews.com)

Antibodies2

• Using the simian immunodeficiency virus (SIV) model, we have taken a markedly different approach: delivery to muscle of an adeno-associated virus gene transfer vector expressing antibodies or antibody-like immunoadhesins having predetermined SIV specificity. (nature.com)
• Figure 3: Serum concentration of immunoadhesins or antibodies after gene transfer and SIV challenge. (nature.com)

Bacterial4

• Metagenomic profiling revealed 8302 bacterial, 395 archaeal, 744 fungal, 524 protist and 1103 viral species in classroom dust. (biomedcentral.com)
• The University of Utah researchers began their examination of the Arc gene by introducing it into bacterial cells. (evesdrift.com)
• Because of the difficulty in distinguishing between the various etiologic agents, both viral and bacterial, causing pneumonia, the workup for symptomatic patients with an infiltrate on chest radiograph should include laboratory studies. (medscape.com)
• BacTag - a pipeline for fast and accurate gene and allele typing in bacterial sequencing data based on database preprocessing. (cdc.gov)

Diseases2

• Novel vaccine technologies: essential components of an adequate response to emerging viral diseases. (nih.gov)
• In addition, GWAS have not fully addressed interactions of genes with disease risk factors such as diet, environmental exposures and infectious diseases. (cdc.gov)

Mutation3

• Patients with sickle cell disease have an inherited mutation in the beta-globin gene, resulting in a faulty hemoglobin structure that yields sickle-shaped red blood cells. (docwirenews.com)
• A number of viral-based vectors are used to deliver genes to correct a known mutation that causes a disease or modulate the immune response against cancer cells. (foxchase.org)
• Wuhan strain will continue to arise, yet not all viral variants generated through mutation of the virus are important or have immediate public health relevance. (who.int)

Anti-viral3

• Inasmuch as the induction of an anti-viral state is a hallmark of IFN responses, we exposed cells to IFN-α, -β, or -γ and challenged with virus. (uab.edu)
• In contrast, MHC and anti-viral defective mutants could not be protected from virus-induced lysis by any IFN. (uab.edu)
• We've been studying this problem for many years, and it's a puzzle we were motivated to solve because cancers with this kind of anti-viral signalling can be particularly aggressive. (sciencealert.com)

Clinical5

• If these infected cells from patients are incubated with RORC2 inhibitors, viral outgrowth was limited, suggesting that RORC2 inhibitors can have a clinical application for the control of HIV-1. (biologists.com)
• We identified an HA gene clade 3C.2a virus as the predominant strain in Gainesville, Florida, USA, during the 2014-15 influenza season and assessed vaccine effectiveness and clinical outcomes associated with this strain. (cdc.gov)
• For all, the consensus viral genomic sequences determined directly from clinical specimens and their matched virus isolates were identical. (cdc.gov)
• As a Univercells company, Exothera capitalizes on novel manufacturing technologies and best-in-class bioprocessing expertise to provide bespoke process optimization and GMP clinical and commercial production of viral vectors. (exothera.world)
• Third, for drug development and toxicities, GWAS continues to provide valuable information on gene-drug interactions with the potential to develop safer and more effective drugs as well as to reduce toxicities in the clinical use of existing medications. (cdc.gov)

Influenza3

• Influenza A(H3N2) strains isolated during 2014-15 in Alachua County, Florida, USA, belonged to hemagglutinin gene clade 3C.2a. (cdc.gov)
• The influenza A(H3N2) virus for the 2014-15 vaccine was an A/Texas/50/2012 (H3N2)-like virus, which is a hemagglutinin (HA) gene clade 3C.1 virus ( 3 - 5 ). (cdc.gov)
• Every influenza virus has eight RNP segments, corresponding to the virus' eight total gene segments. (cdc.gov)

Sequences1

• They could 1) recall all CaMV Gene VI-containing crops (in Europe that would mean revoking importation and planting approvals) or, 2) undertake a retrospective risk assessment of the CaMV promoter and its Gene VI sequences and hope to give it a clean bill of health. (greenmedinfo.com)

Polymerase chain re1

• Polymerase chain reaction (PCR) Genetic diagnostic technologies are scientific methods that are used to understand and evaluate an organism's genes. (merckmanuals.com)

Pneumonia1

• Viral pneumonia can be diagnosed by isolation and identification of the pathogen through viral culture. (medscape.com)

CRISPR8

• A large part of the attraction of CRISPR stems from the small size of its two core components - Cas9 and gRNA - and hence its compatibility with virtually any available viral vector delivery system. (semanticscholar.org)
• The recent advances in the development of non-viral delivery systems for CRISPR-Cas9 are surveyed and challenges and future directions in this field are discussed. (semanticscholar.org)
• CRISPR-CAS9 is a gene-editing tool that makes a precise cut in a DNA strand, and then the normal DNA repair process takes over to incorporate a newly inserted gene into the DNA. (merckmanuals.com)
• Although viral vectors have been widely used in the delivery of the CRISPR/Cas9 system in vitro and in vivo, their fundamental shortcomings, such as the risk of carcinogenesis, limited insertion size, immune responses and difficulty in large-scale production, severely limit their further applications. (nih.gov)
• Alternative non-viral delivery systems for CRISPR/Cas9 are urgently needed. (nih.gov)
• With the rapid development of non-viral vectors, lipid- or polymer-based nanocarriers have shown great potential for CRISPR/Cas9 delivery. (nih.gov)
• Furthermore, current non-viral vectors that have been applied for CRISPR/Cas9 delivery in vitro and in vivo are outlined in details. (nih.gov)
• Finally, critical obstacles for non-viral delivery of CRISPR/Cas9 system are highlighted and promising strategies to overcome these barriers are proposed. (nih.gov)

Cells17

• When tested in animal models, this vector was able to incorporate correct genes into bone marrow stem cells up to 10 times more efficiently than current vectors. (docwirenews.com)
• These blood-producing cells are altered to possess a normal copy of the beta-globin gene in a lab and are then reinfused into the patient, ultimately inducing the production of healthy red blood cells. (docwirenews.com)
• To detect this, the authors performed chromatin immunoprecipitation of RORC2 in HIV-1 infected Jurkat cells and observed that RORC2 does bind to integrated viral DNA. (biologists.com)
• Lastly, analysis of memory T cells from patients with HIV-1 showed that cells that express RORC2 are enriched in viral DNA. (biologists.com)
• This gene helps produce ACE2 receptors on the surface of human cells. (medscape.com)
• Another method for inserting genes uses liposomes, which are microscopic sacs containing the DNA that are absorbed by the person's cells, thereby delivering their DNA to the cell nucleus. (merckmanuals.com)
• Sometimes this method does not work because the liposomes are not absorbed into the person's cells, the new gene does not work as intended, or the new gene is eventually lost. (merckmanuals.com)
• Two independent teams of NIH-funded researchers discovered that the Arc gene can package its genetic material in a virus-like shell for delivery to nearby cells. (evesdrift.com)
• Viral NA b inhibition prevents sialic acid cleavage from HA beta virus gets trapped inside cells, and epithelial spread is blocked. (medscape.com)
• Cells are removed from the patient, genetically modified (often using a viral vector) and then returned to the patient. (fda.gov)
• In total, 446 drugs were screened for their viral sensitizing properties in glioblastoma stem-like cells (GSCs) in vitro. (eur.nl)
• The WHO Monitoring Group on Gene transfer Medicinal Products was established to monitor devel- opments and draw up appropriate guidance for assuring the quality of gene transfer medicinal prod- ucts, including nucleic acids, viral and non-viral vectors, and genetically modified cells. (who.int)
• The aim of this Master's thesis was to study non-viral gene delivery to RPE cells and endothelial cells using several carrier/DNA combinations. (helsinki.fi)
• The number of HPV viral copies was normalized to the number of cells in the PCR reaction as estimated by a real-time PCR assay to a single copy human gene (ERV-3). (cdc.gov)
• The viral load of H1N1 and RSV was significantly elevated in CS/virus-exposed mice and NCI-H292 cells after Fluticasone and Dexamethasone treatment. (uni-wuerzburg.de)
• The ability of cancer cells to specifically instruct the fibroblasts to expose the viral-like end of RN7SL1 is a key discovery," says Minn . (sciencealert.com)
• Epigenetic Alteration of the Cancer-Related Gene TGFBI in B Cells Infected with Epstein-Barr Virus and Exposed to Aflatoxin B1: Potential Role in Burkitt Lymphoma Development. (who.int)

Infectious1

• The foreign amino acids are displayed on the surface, allowing fusion phage bearing antigenic determinants from a target gene to be purified in infectious form by affinity to antibody directed against the gene product. (nih.gov)

Transfection1

• Gene delivery did not succeed with LCDCs in any transfection. (helsinki.fi)

Strains2

• T-cell competition takes two forms, per Deem's computer models: original antigenic sin (also known as "deceptive imprinting"), and immunodominance, which occurs when several viral strains simultaneously infect a single person. (aps.org)
• Overall, this study identified four viral sensitizers, which synergize with Delta24-RGD and two other strains of OVs. (eur.nl)

Human9

• So it is possible to swap entire sets of genetic code, including the genes that allow bacteria to develop resistance to antibiotics-and for the human immune system to continually adapt to invasive species, an attribute Deem has traced to an HGT insertion that occurred some 400 million years ago. (aps.org)
• This is because there are clear indications that this viral gene (called Gene VI) might not be safe for human consumption. (greenmedinfo.com)
• In general, viral genes expressed in plants raise both agronomic and human health concerns (reviewed in Latham and Wilson 2008 ). (greenmedinfo.com)
• Gene transfer into human leukemia cell lines by electroporation: experience with exponentially decaying and square wave pulse. (nature.com)
• Research from our lab and others has demonstrated that HERV genes are active in diseased tissue, such as tumors , as well as during human embryonic development . (the-scientist.com)
• MicroRNAs regulate at least one third of all human genes and are phylogenetically well conserved. (cdc.gov)
• While the principles endorse somatic cell gene editing and the development of regulatory standards for gene editing, the document asserts that it is too early to support any form of human germline gene editing due to unanswered ethical, legal and safety questions. (genetherapynet.com)
• Signals in human leukocyte antigen (HLA) class II region dominated the landscape of viral antibody response, with 40 independent loci and 14 independent classical alleles, 7 of which exhibited pleiotropic effects across viral families. (medrxiv.org)
• EMCV is used in laboratory mice to model the symptoms and pathogenesis of human type I diabetes and viral myocarditis ( 2 , 3 ). (cdc.gov)

Improving viral vector1

• This featured report explores strategies for improving viral vector manufacturing. (bioprocessintl.com)

Transcriptional2

• EBNA 2 is a transcriptional regulator of both viral and cellular genes. (sussex.ac.uk)
• To study the regulation of Cp-transcript elongation, we used a pair of EBV-infected cell-lines to compare the transcriptional complexes associated with the Cp transcriptional unit and two shorter EBNA 2-regulated viral genes, LMP1 and LMP2A. (sussex.ac.uk)

Bacteria2

• But in the laboratory, the scientists discovered that Staphylococcus aureus, the bug that causes toxic shock syndrome among other things, can actually co-opt phage, using them to shuttle the gene for toxic-shock toxin to another bacteria, in this case Listeria . (scientificamerican.com)
• The process of genetic modification, which takes place in a laboratory, typically merges DNA from different species, creating combinations of plant, animal, bacteria and viral genes that cannot occur in nature or in traditional crossbreeding. (nongmoproject.org)

Researchers5

• For this study, researchers used transposons to deliver an antibiotic-resistant gene. (technologynetworks.com)
• This new viral vector also has a carrying capacity up to six times higher than the conventional vector, as per the researchers. (docwirenews.com)
• The researchers note that this complicated gene translation process has made both the preparation of the vector and the gene-transfer efficiency more challenging, however. (docwirenews.com)
• Researchers at the University of Wisconsin-Madison have packed a gene-editing payload into a customizable, synthetic nanocapsule. (wisc.edu)
• Researchers at the University of Wisconsin-Madison have addressed many of those problems by packing a gene-editing payload into a tiny, customizable, synthetic nanocapsule. (genetherapynet.com)

Level of viral1

• This limitation is overcome by using quantitative PCR, which shows the level of viral load. (medscape.com)

Detection of viral2

• Stool samples from an outbreak that are submitted to CDC for detection of viral pathology should be collected in bulk from 10 ill persons during their first 48 hours of illness, while feces are still liquid, and should be stored at 4 C (not frozen). (cdc.gov)
• However, the detection of viral pathogens does not always indicate active disease. (medscape.com)

Particles2

• Figure 1: Example of viral particles used as vectors for gene delivery. (mpg.de)
• We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services. (amsbio.com)

Encodes1

• In the course of analysis to identify potential allergens in GMO crops, the European Food Safety Authority (EFSA) has belatedly discovered that the most common genetic regulatory sequence in commercial GMOs also encodes a significant fragment of a viral gene (Podevin and du Jardin 2012) . (greenmedinfo.com)

Disease3

• What began as an effort to examine the behavior of a gene involved in memory and implicated in neurological disorders such as Alzheimer's disease has unexpectedly led to the discovery of an entirely new process, which neurons may use to send genetic information to one another. (evesdrift.com)
• Genes that don't work properly can cause disease. (swfinstitute.org)
• Unfortunately, diagnostic technology is insufficiently developed to permit determining the disease burden of each of the known viral pathogens. (cdc.gov)

Respiratory1

• Tissue from the upper or lower respiratory tract, sputum samples, and samples obtained by nasopharyngeal washing, bronchoalveolar lavage, and biopsy may be submitted for viral culture. (medscape.com)

Pathogens1

• Viral cultures are still the criterion standard for most viral pathogens, but they take a long time to complete. (medscape.com)

Capsid1

• ORF-2 contains genes encoding the capsid. (medscape.com)

Common viral1

• In 1972, in the examination of stool specimens, electron microscopy identified the Norwalk agent, the most common viral cause of gastroenteritis outbreaks among adults. (cdc.gov)

Genetically modified1

• Agdia, Inc. (Elkhart, IN) has announced an expanded field of use for their AmplifyRP ® isothermal amplification technology that now allows detection of genetically modified traits and native plant genes. (agdia.com)

Tissues1

• How long are the delivered genes expressed in the treated tissues? (medgadget.com)

Nonstructural1

• The positive samples were further subjected to PCRs for the amplification of a partial segment of the Usutu virus envelope and nonstructural 5 gene. (cdc.gov)

Treatments1

• Treatments using HERV genes as a cancer biomarker will also need to take into account their activity in healthy tissue. (the-scientist.com)

Pathogen2

• This is because many viral genes function to disable their host in order to facilitate pathogen invasion. (greenmedinfo.com)
• We believe our experiences and successes in development of isothermal plant pathogen assays will provide a seamless path for detection of plant genes. (agdia.com)

Vaccine2

• In anticipation of vaccine development and use, CDC recently began national surveillance for the viral agents of gastroenteritis. (cdc.gov)
• Adenoviruses are not the only viral vectors that can be used: pharmaceutical giant Merck says it is working on a potential COVID vaccine using an engineered vesicular stomatis virus , previously used successfully in its Ebola vaccine. (allianceforscience.org)

Virus8

• As examples, one patient died because of his immune response to an adenovirus and three children in another study developed leukemia because the virus inserted itself upstream of a cancer-causing gene. (technologynetworks.com)
• This approach entails that the genes incorporated into the virus are translated from right to left by the enzymes, analogous to a sentence being read backward. (docwirenews.com)
• Beforehand, if I had said to any neuroscientist that this gene sort of acts like a virus, they would have laughed at me," said Jason Shepherd, Ph.D., an assistant professor at the University of Utah in Salt Lake City, Utah. (evesdrift.com)
• Afin de remédier à ce problème et d'étudier les variations génétiques et antigéniques des virus A(H1N1)pdm09 et H3N2, nous avons procédé à des analyses génétiques et phylogénétiques des gènes de l'hémagglutinine (HA) et de la neuraminidase (NA) de ces virus, sur la période 2011-2013 en Jordanie. (who.int)
• L'analyse a porté sur les séquences complètes des gènes de l'HA et de la NA de 16 échantillons positifs au virus H1N1 prélevés dans le cadre de cette étude, ainsi que sur 21 séquences publiées de l'HA et 20 séquences publiées de la NA, issues de virus jordaniens disponibles sur les bases de données de gènes en ligne. (who.int)
• Unidades funcionales de la herencia en los VIRUS. (bvsalud.org)
• Preliminary results indicate that the absence of the Us9 gene, not previously known to affect pathogenesis, has striking effects on virus spread. (vetres.org)
• We conclude that at least three ADV genes (gE, gI and Us9) are all necessary, but each is not sufficient to sponsor anterograde directed spread of the virus in specific rodent neurons. (vetres.org)