Recombinases: A broad category of enzymes that are involved in the process of GENETIC RECOMBINATION.Gene Transfer, Horizontal: The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).Integrases: Recombinases that insert exogenous DNA into the host genome. Examples include proteins encoded by the POL GENE of RETROVIRIDAE and also by temperate BACTERIOPHAGES, the best known being BACTERIOPHAGE LAMBDA.DNA Nucleotidyltransferases: Enzymes that catalyze the incorporation of deoxyribonucleotides into a chain of DNA. EC 2.7.7.-.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Charities: Social welfare organizations with programs designed to assist individuals in need.Uterine Cervical Neoplasms: Tumors or cancer of the UTERINE CERVIX.Papillomavirus Infections: Neoplasms of the skin and mucous membranes caused by papillomaviruses. They are usually benign but some have a high risk for malignant progression.Penile Neoplasms: Cancers or tumors of the PENIS or of its component tissues.Vaginal Neoplasms: Tumors or cancer of the VAGINA.Great BritainOropharyngeal Neoplasms: Tumors or cancer of the OROPHARYNX.Caulimovirus: A genus of PLANT VIRUSES, in the family CAULIMOVIRIDAE, that are transmitted by APHIDS in a semipersistent manner. Aphid-borne transmission of some caulimoviruses requires certain virus-coded proteins termed transmission factors.European Union: The collective designation of three organizations with common membership: the European Economic Community (Common Market), the European Coal and Steel Community, and the European Atomic Energy Community (Euratom). It was known as the European Community until 1994. It is primarily an economic union with the principal objectives of free movement of goods, capital, and labor. Professional services, social, medical and paramedical, are subsumed under labor. The constituent countries are Austria, Belgium, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, and the United Kingdom. (The World Almanac and Book of Facts 1997, p842)Food Safety: Activities involved in ensuring the safety of FOOD including avoidance of bacterial and other contamination.Mosaic Viruses: Viruses which produce a mottled appearance of the leaves of plants.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Manuscripts, MedicalPlants, Genetically Modified: PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.Cosmic Dust: Finely divided solid matter with particle sizes smaller than a micrometeorite, thus with diameters much smaller than a millimeter, moving in interplanetary space. (NASA Thesaurus, 1994)Meteoroids: Any solid objects moving in interplanetary space that are smaller than a planet or asteroid but larger than a molecule. Meteorites are any meteoroid that has fallen to a planetary surface. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Stars, Celestial: Large bodies consisting of self-luminous gas held together by their own gravity. (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed)Solar System: The group of celestial bodies, including the EARTH, orbiting around and gravitationally bound by the sun. It includes eight planets, one minor planet, and 34 natural satellites, more than 1,000 observed comets, and thousands of lesser bodies known as MINOR PLANETS (asteroids) and METEOROIDS. (From Academic American Encyclopedia, 1983)Spacecraft: Devices, manned and unmanned, which are designed to be placed into an orbit about the Earth or into a trajectory to another celestial body. (NASA Thesaurus, 1988)Nucleoside-Phosphate Kinase: An enzyme that catalyzes reversible reactions of a nucleoside triphosphate, e.g., ATP, with a nucleoside monophosphate, e.g., UMP, to form ADP and UDP. Many nucleoside monophosphates can act as acceptor while many ribo- and deoxyribonucleoside triphosphates can act as donor. EC 2.7.4.4.Television: The transmission and reproduction of transient images of fixed or moving objects. An electronic system of transmitting such images together with sound over a wire or through space by apparatus that converts light and sound into electrical waves and reconverts them into visible light rays and audible sound. (From Webster, 3rd ed)Heavy Ion Radiotherapy: The use of a heavy ion particle beam for radiotherapy, such as the HEAVY IONS of CARBON.Proton Therapy: The use of an external beam of PROTONS as radiotherapy.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Dependovirus: A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.Transduction, Genetic: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Antiviral Agents: Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly.Influenza A virus: The type species of the genus INFLUENZAVIRUS A that causes influenza and other diseases in humans and animals. Antigenic variation occurs frequently between strains, allowing classification into subtypes and variants. Transmission is usually by aerosol (human and most non-aquatic hosts) or waterborne (ducks). Infected birds shed the virus in their saliva, nasal secretions, and feces.Influenza, Human: An acute viral infection in humans involving the respiratory tract. It is marked by inflammation of the NASAL MUCOSA; the PHARYNX; and conjunctiva, and by headache and severe, often generalized, myalgia.Vaccines: Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.Orthomyxoviridae Infections: Virus diseases caused by the ORTHOMYXOVIRIDAE.Influenza A Virus, H1N1 Subtype: A subtype of INFLUENZA A VIRUS with the surface proteins hemagglutinin 1 and neuraminidase 1. The H1N1 subtype was responsible for the Spanish flu pandemic of 1918.Orthomyxoviridae: A family of RNA viruses causing INFLUENZA and other diseases. There are five recognized genera: INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; ISAVIRUS; and THOGOTOVIRUS.Fever of Unknown Origin: Fever in which the etiology cannot be ascertained.Emergency Service, Hospital: Hospital department responsible for the administration and provision of immediate medical or surgical care to the emergency patient.Genetic Testing: Detection of a MUTATION; GENOTYPE; KARYOTYPE; or specific ALLELES associated with genetic traits, heritable diseases, or predisposition to a disease, or that may lead to the disease in descendants. It includes prenatal genetic testing.Pick Disease of the Brain: A rare form of DEMENTIA that is sometimes familial. Clinical features include APHASIA; APRAXIA; CONFUSION; ANOMIA; memory loss; and personality deterioration. This pattern is consistent with the pathologic findings of circumscribed atrophy of the poles of the FRONTAL LOBE and TEMPORAL LOBE. Neuronal loss is maximal in the HIPPOCAMPUS, entorhinal cortex, and AMYGDALA. Some ballooned cortical neurons contain argentophylic (Pick) bodies. (From Brain Pathol 1998 Apr;8(2):339-54; Adams et al., Principles of Neurology, 6th ed, pp1057-9)Emergencies: Situations or conditions requiring immediate intervention to avoid serious adverse results.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Wounds and Injuries: Damage inflicted on the body as the direct or indirect result of an external force, with or without disruption of structural continuity.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Pregnancy Proteins: Proteins produced by organs of the mother or the PLACENTA during PREGNANCY. These proteins may be pregnancy-specific (present only during pregnancy) or pregnancy-associated (present during pregnancy or under other conditions such as hormone therapy or certain malignancies.)Pregnancy Trimester, First: The beginning third of a human PREGNANCY, from the first day of the last normal menstrual period (MENSTRUATION) through the completion of 14 weeks (98 days) of gestation.Pregnancy Complications: Conditions or pathological processes associated with pregnancy. They can occur during or after pregnancy, and range from minor discomforts to serious diseases that require medical interventions. They include diseases in pregnant females, and pregnancies in females with diseases.Pregnancy Outcome: Results of conception and ensuing pregnancy, including LIVE BIRTH; STILLBIRTH; SPONTANEOUS ABORTION; INDUCED ABORTION. The outcome may follow natural or artificial insemination or any of the various ASSISTED REPRODUCTIVE TECHNIQUES, such as EMBRYO TRANSFER or FERTILIZATION IN VITRO.Pregnancy Trimester, Third: The last third of a human PREGNANCY, from the beginning of the 29th through the 42nd completed week (197 to 294 days) of gestation.Pregnancy, Animal: The process of bearing developing young (EMBRYOS or FETUSES) in utero in non-human mammals, beginning from FERTILIZATION to BIRTH.

Four dimers of lambda repressor bound to two suitably spaced pairs of lambda operators form octamers and DNA loops over large distances. (1/11892)

Transcription factors that are bound specifically to DNA often interact with each other over thousands of base pairs [1] [2]. Large DNA loops resulting from such interactions have been observed in Escherichia coli with the transcription factors deoR [3] and NtrC [4], but such interactions are not, as yet, well understood. We propose that unique protein complexes, that are not present in solution, may form specifically on DNA. Their uniqueness would make it possible for them to interact tightly and specifically with each other. We used the repressor and operators of coliphage lambda to construct a model system in which to test our proposition. lambda repressor is a dimer at physiological concentrations, but forms tetramers and octamers at a hundredfold higher concentration. We predict that two lambda repressor dimers form a tetramer in vitro when bound to two lambda operators spaced 24 bp apart and that two such tetramers interact to form an octamer. We examined, in vitro, relaxed circular plasmid DNA in which such operator pairs were separated by 2,850 bp and 2,470 bp. Of these molecules, 29% formed loops as seen by electron microscopy (EM). The loop increased the tightness of binding of lambda repressor to lambda operator. Consequently, repression of the lambda PR promoter in vivo was increased fourfold by the presence of a second pair of lambda operators, separated by a distance of 3,600 bp.  (+info)

A cytomegalovirus glycoprotein re-routes MHC class I complexes to lysosomes for degradation. (2/11892)

Mouse cytomegalovirus (MCMV) early gene expression interferes with the major histocompatibility complex class I (MHC class I) pathway of antigen presentation. Here we identify a 48 kDa type I transmembrane glycoprotein encoded by the MCMV early gene m06, which tightly binds to properly folded beta2-microglobulin (beta2m)-associated MHC class I molecules in the endoplasmic reticulum (ER). This association is mediated by the lumenal/transmembrane part of the protein. gp48-MHC class I complexes are transported out of the ER, pass the Golgi, but instead of being expressed on the cell surface, they are redirected to the endocytic route and rapidly degraded in a Lamp-1(+) compartment. As a result, m06-expressing cells are impaired in presenting antigenic peptides to CD8(+) T cells. The cytoplasmic tail of gp48 contains two di-leucine motifs. Mutation of the membrane-proximal di-leucine motif of gp48 restored surface expression of MHC class I, while mutation of the distal one had no effect. The results establish a novel viral mechanism for downregulation of MHC class I molecules by directly binding surface-destined MHC complexes and exploiting the cellular di-leucine sorting machinery for lysosomal degradation.  (+info)

Novel endotheliotropic herpesviruses fatal for Asian and African elephants. (3/11892)

A highly fatal hemorrhagic disease has been identified in 10 young Asian and African elephants at North American zoos. In the affected animals there was ultrastructural evidence for herpesvirus-like particles in endothelial cells of the heart, liver, and tongue. Consensus primer polymerase chain reaction combined with sequencing yielded molecular evidence that confirmed the presence of two novel but related herpesviruses associated with the disease, one in Asian elephants and another in African elephants. Otherwise healthy African elephants with external herpetic lesions yielded herpesvirus sequences identical to that found in Asian elephants with endothelial disease. This finding suggests that the Asian elephant deaths were caused by cross-species infection with a herpesvirus that is naturally latent in, but normally not lethal to, African elephants. A reciprocal relationship may exist for the African elephant disease.  (+info)

The haplotype distribution of two genes of citrus tristeza virus is altered after host change or aphid transmission. (4/11892)

Genetic variability of citrus tristeza virus (CTV) was studied using the haplotypes detected by single-strand conformation polymorphism (SSCP) analysis of genes p18 and p20 in six virus populations of two origins. The Spanish group included a CTV isolate and subisolates obtained by graft-transmission to different host species. The other included two subisolates aphid-transmitted from a single Japanese isolate. The homozygosity observed for gene p20 was always significantly higher than that expected under neutral evolution, whereas only three populations showed high homozygosity for p18, suggesting stronger host constraints for p20 than for p18. Sequential transmissions of a Spanish isolate to new host species increased the difference between its population and that of the successive subisolates for gene p18, as estimated by the F statistic. Analysis of molecular variance indicated that variation between both groups of populations was not statistically significant, whereas variations between populations of the same group or within populations were significant for both genes studied. Our data indicate that selection affects the haplotype distribution and that adaptation to a new host can be as important or more as the geographical origin. Variation of the CTV populations after host change or aphid transmission may explain in part the wide biological variability observed among CTV isolates.  (+info)

Bacteriophage SPO1 development: defects in a gene 31 mutant. (5/11892)

SPO1 temperature-sensitive mutant ts14-1, located in cistron 31, has a DD (DNA synthesis-delayed) phenotype at 37 degrees C and produces progeny in a stretched program. At 44 degrees C it behaves as a DO (DNA synthesis-defective) mutant and shuts off the viral RNA synthesis about 10 min after infection. The thermal sensitivity of this mutant is due to the inactivity of gp-31 (the product of gene 31) at 44 degrees C. However, gp-31 is synthesized at that temperature and partly recovers its activity at 37 degrees C. Only 5 min at the permissive temperature is enough to trigger the continuation of the phage program and to produce progeny. The partial defect at 37 degrees C and the expansion of the middle program together with the pleiotropic defects at the nonpermissive temperature could be suitable for the study of the controls involved in bacteriophage development.  (+info)

Evidence that the neck appendages are adsorption organelles in Bacillus subtilis bacteriophage phi29. (6/11892)

A mutant of Bacillus subtilis unable to adsorb phage phi29 efficiently has been isolated. This mutant can be infected by host range mutants of the phage. Since the host range mutations map in cistron 12, which codes for neck appendage protein, this would tend to confirm that these organelles are involved in viral adsorption.  (+info)

Control of corynebacteriophage reproduction by heteroimmune repression. (7/11892)

Corynebacteriophages beta and gamma are closely related but heteroimmune; hence, gamma reproduces in C7(beta). A series of gamma mutants, designated gamma-bin (beta-inhibited), has been isolated. They reproduce in only 2 to 14% of infected C7(beta) cells, and, as a result, plaque with an efficiency of 10(-4) to 10(-5) on this strain. The proportion of C7(beta) cells in which gamma-bin phage can replicate is increased to 30 to 80% when immunity is lifted by UV induction of C7(beta) or by heat induction of C7(beta-tsr3). The gamma-bin mutants carry out a normal vegetative or lysogenic cycle in strain C7 and thus do not appear to be defective in any essential phage function. Infection of C7(beta) by gamma-bin results in cell killing whether the infection is productive or nonproductive. The data support the hypothesis that inhibition of gamma-bin is due to the direct or indirect action of a beta prophage gene. The simplest hypothesis is that gamma-bin phages have sustained mutations in an operator site and that beta repressor now combines with the mutated operator to inhibit normal replication in a significant proportion of infected cells.  (+info)

Correlated genetic and EcoRI cleavage map of Bacillus subtilis bacteriophage phi105 DNA. (8/11892)

The seven previously identified EcoRI cleavage fragments of phi 105 DNA were ordered with respect to their sites of origin on the phage genome by marker rescue. One fragment, H, did not carry any determinants essential for replication. This fragment was totally missing in a deletion mutant which exhibited a lysogenization-defective phenotype. There is a nonessential region on the phi 105 genome which begins in fragment B, spans fragment H, and ends in fragment F. The size of the nonessential region, as estimated by alterations observed in the fragmentation patterns of deletion mutant DNAs, is approximately 2.7 X 10(6) daltons. Two new EcoRI cleavage fragments with molecular weights of approximately 0.2 X 10(6) were detected by autoradiography of 32P-labeled DNA. These small fragments were not located on the cleavage map.  (+info)

SARC021C is a nonrandomized, open-label, multicenter, continuation study designed to provide access to TH-302 for patients currently receiving and benefiting
To verify the assignment of performance of a distinct viral gene, it is actually probably needed to restore the mutation back to the wild form sequence and deter mine irrespective of whether the phenotype in the rescuant viruses is similar to that of the parental virus. Having said that, the rescue procedures could potentially introduce adventitious muta tions that arise elsewhere in the genome. Meanwhile, it truly is doable the deletion of the target ORF could possibly impact the expression of other viral genes, like individuals in close by regions, since the deleted area may well func tion as a regulatory component vital for that expression of those genes, additionally to encoding the target ORF. Substantial research are necessary to demonstrate that the dele tion does not affect every other gene expression in the viral genome.. Alternatively, a viral mutant that contains a sub tle mutation, such as stage mutations, to inactivate the ORF could be info generated. Examination with the phenotype ...
A map cells reference object encapsulates the relationship between a planar map coordinate system and a system of intrinsic coordinates anchored to the columns and rows of a 2-D spatially referenced raster grid or image.
Ichnovirus is a genus of viruses, in the family Polydnaviridae. Parasitoid wasps serve as hosts, but these wasps are themselves parasites of lepidoptera. There are currently 21 species in this genus including the type species Campoletis sonorensis ichnovirus. Group: dsDNA Order: Unassigned Family: Polydnaviridae Genus: Ichnovirus Campoletis aprilis ichnovirus Campoletis flavicincta ichnovirus Campoletis sonorensis ichnovirus Casinaria arjuna ichnovirus Casinaria forcipata ichnovirus Casinaria infesta ichnovirus Diadegma acronyctae ichnovirus Diadegma interruptum ichnovirus Diadegma terebrans ichnovirus Enytus montanus ichnovirus Eriborus terebrans ichnovirus Glypta fumiferanae ichnovirus Hyposoter annulipes ichnovirus Hyposoter exiguae ichnovirus Hyposoter fugitivus ichnovirus Hyposoter lymantriae ichnovirus Hyposoter pilosulus ichnovirus Hyposoter rivalis ichnovirus Olesicampe benefactor ichnovirus Olesicampe geniculatae ichnovirus Synetaeris tenuifemur ichnovirus Viruses in Ichnovirus are ...
Endogenous mouse mammary tumor virus DNA is distributed among multiple mouse chromosomes. Journal of Law, Medicine and Ethics. 1979 ...
Experimentally introduced defective endogenous proviruses are highly expressed in chickens.: We have previously described the experimental introduction of recom
VIRUS GENES publishesstudies on analysis of virus genes, gene products and functions, regulation of virus gene function, cell biology of virus infectionfunctional studies of genes and gene families, encoded by eukaryotic, ...
VIRUS GENES publishesstudies on analysis of virus genes, gene products and functions, regulation of virus gene function, cell biology of virus infectionfunctional studies of genes and gene families, encoded by eukaryotic, ...
Researchers have discovered a hidden viral gene lurking in many commercial GMO crops, raising possible concerns about its impact on human health.
During the lytic cycle, proviruses are created by integrating viral genetic information within the host cells genetic information. is this true or false? ...
TY - JOUR. T1 - Function of herpes simplex virus gene products. AU - Nishiyama, Y.. AU - Murata, Takayuki. AU - Yamauchi, Y.. PY - 2001/1/1. Y1 - 2001/1/1. UR - http://www.scopus.com/inward/record.url?scp=0035380417&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0035380417&partnerID=8YFLogxK. U2 - 10.2222/jsv.51.29. DO - 10.2222/jsv.51.29. M3 - Review article. C2 - 11565262. AN - SCOPUS:0035380417. VL - 51. SP - 29. EP - 36. JO - Uirusu. Journal of virology. JF - Uirusu. Journal of virology. SN - 0042-6857. IS - 1. ER - ...
We have determined the DNA sequence of the long repeat region (RL) in the genome of herpes simplex virus type 1 (HSV-1) strain 17, as 9215 bp of composition 71.6% G + C. In addition, the sequences of parts of the long unique region (UL) adjacent to the terminal (TRL) and internal (IRL) copies of RL …
Zalckvar, E., C. Paulus, D. Tillo, A. Asbach-Nitzsche, Y. Lubling, iv, C. Winterling, N. Strieder, K. Mücke, F. Goodrum, E. Segal, et al., Nucleosome maps of the human cytomegalovirus genome reveal a temporal switch in chromatin organization linked to a major IE protein., Proc Natl Acad Sci U S A, vol. 110, issue 32, pp. 13126-31, 2013 Aug 6. PMCID: PMC3740854 PMID: 23878222 ...
Note that this code wont work out of the box for the map shown in your question. This is because you have the odd tiles offset to the left, whereas the odd tile is more usually offset to the right (As is the case in the tiled map editor). You should be able to easy remedy this by tweaking the x value returned in the odd-tile case.. Explanation. This may seem to be a slightly more brute-force method of accomplishing this task, but it does at least have the advantage of being pixel perfect and slightly more flexible.. The trick is in viewing the map not as a single staggered grid, but as two grids overlayed on top of one another. Theres the odd-rows grid and the even-rows grid, but lets call them red and green instead so that we can create a pretty diagram.... ...
Health, ...[Boston MA June 17 2014]─ Sarcopenia the significant loss of ...When people strength train the body responds by making muscle. The res... In order for the body to make proteins that build muscle certain gen...Rivas and colleagues observed that the level of microRNAs small RNA m...,Researchers,identify,mechanism,that,could,help,old,muscle,grow,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
TY - JOUR. T1 - Identification and characterization of the virion-induced host shutoff product of herpes simplex virus gene UL41. AU - Smibert, C. A.. AU - Johnson, David. AU - Smiley, J. R.. PY - 1992. Y1 - 1992. N2 - The virion-induced host shutoff product of the herpes simplex virus UL41 gene is required for shutoff of host translation and degradation of cellular mRNAs. We employed a rabbit antipeptide antiserum to identify a 58K UL41-related phosphoprotein in infected cells. We also provide evidence that this protein is a component of the virus particle, consistent with its role in virion-induced shutoff.. AB - The virion-induced host shutoff product of the herpes simplex virus UL41 gene is required for shutoff of host translation and degradation of cellular mRNAs. We employed a rabbit antipeptide antiserum to identify a 58K UL41-related phosphoprotein in infected cells. We also provide evidence that this protein is a component of the virus particle, consistent with its role in ...
Efficient, accurate and convenient foreign-gene insertion strategies are crucial for the high-throughput and rapid construction of large DNA viral vectors, but relatively inefficient and labour-intensive methods have limited the application of recombinant viruses. In this study, we applied the nonhomologous insertion (NHI) strategy, which is based on the nonhomologous end joining (NHEJ) repair pathway. Compared to the currently used homologous recombination (HR) strategy, we obtained a higher efficiency of foreign-gene insertion into the herpes simplex virus (HSV) genome that reached 45 % after optimization. By using NHI, we rapidly constructed recombinant reporter viruses using a small amount of clinical viruses, and the recombinant virus was stable for at least ten consecutive passages. The fidelity of NHI ranged from 70-100% and was related to the sequence background of the insertion site according to the sequencing results. Finally, we depict the dynamic process by which the foreign-gene donor
Researchers led by John F. DiPersio, MD, PhD, at the School of Medicine have designed a way to mitigate graft-versus-host disease, a common and often life-threatening complication of bone marrow transplants that are used to treat leukemia and other blood cancers. The method also employs a molecular imaging tool to help doctors identify patients most likely to develop this dangerous condition ...
This is a guest post by JoeCoder. Its often argued that when two or more organisms share viral genes in the same place, it is evidence those organisms evolved from a common ancestor. Wikipedias Evidence for Common Descent page frames it as follows: Endogenous retroviruses (or ERVs) are remnant sequences in the genome left from…
Here is what the Symantec Anti-virus Research Center has on this virus: http://www.sarc.com/avcenter/venc/data/[email protected] -----Original Message----- From: [email protected] [mailto:[email protected]] Sent: Tuesday, September 04, 2001 4:44 PM To: [email protected] Subject: [802.3ae] Virus Alert InterScan has detected a virus TROJ_APOST.A in the file (readme.exe) in the mail traffic sent to you by [email protected] ...
vaccinia virus nicking-joining enzyme: virus-specific, DNA-dependent & does not require ATP; possesses both endonuclease & ligase activities
TY - JOUR. T1 - Monitoring of ganciclovir sensitivity of multiple human cytomegalovirus strains coinfecting blood of an AIDS patient by an immediate-early antigen plaque assay. AU - Gerna, Giuseppe. AU - Baldanti, Fausto. AU - Zavattoni, Maurizio. AU - Sarasini, Antonella. AU - Percivalle, Elena. AU - Revello, M. Grazia. PY - 1992/10/1. Y1 - 1992/10/1. N2 - A plaque-reduction assay for chemosensitivity testing of human cytomegalovirus (HCMV) strains was developed based on early detection of viral plaques 96 h p.i. by a monoclonal antibody to the major immediate-early protein p72. Sequential HCMV isolates from an AIDS patient undergoing multiple courses of ganciclovir treatment during an 18-month follow-up were tested by the new assay, showing emergence of a ganciclovir-resistant strain. However, cloning of viral isolates and Southern blot hybridization analysis showed the simultaneous presence of three different HCMV strains in blood. Of these, the resistant strain was likely to be selected ...
The 5 nucleotide sequences of the transforming gene of simian sarcoma virus (v-sis) and its human cellular homolog (c-sis) were compared. A short homology was found between helper virus and cellular DNA sequences at the junction of v-sis and c-sis, which may have had a role in the original recombination event leading to the generation of simian sarcoma virus. ...
T cells that recognize self antigen are clonally deleted in the thymus--a maturation process that occurs in the context of histocompatibility molecules and the T-cell receptor. The minor lymphocyte stimulation antigens (Mls) effect these deletions through interactions with the V beta portion of the T-cell receptor, thus mimicking bacterial superantigens. Intrigued by the fact that each known Mls gene maps to the same chromosomal region as an endogenous mouse mammary tumour virus (Mtv), we reevaluated the linkage relationships between the two gene families. Here we report perfect concordance in inbred and recombinant inbred mice between the presence of four Mtv proviruses with the expression of Mls gene products. These data suggest a general model in which mammary tumour virus gene products themselves are the ligands that shape a considerable portion of the immunological repertoire of common laboratory mice
Sigma-Aldrich offers abstracts and full-text articles by [Zhihua Li, Yanwei Bi, Hongjian Xiao, Le Sun, Yuan Ren, Yadong Li, Chen Chen, Wei Cun].
Genome map of SSV1. Open reading frames are shown as block arrows and labeled as in Palm et al. (1991). Virus structural protein genes (Reiter et al., 1987a) an
Fischinger, P J.; Thiel, H J.; Lieberman, M; Kaplan, H S.; Dunlop, N M.; and Robey, W G., "Presence of a novel recombinant murine leukemia virus-like glycoprotein on the surface of virus-negative c57bl lymphoma cells." (1982). Subject Strain Bibliography 1982. 3615 ...
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The world has been waiting patiently for a solution to the perpetual motion machine problem. Leave it to the Chinese to solve it. Now, where the hell is my flying car Popular Science has been promising me for 50 years? I want mine to be electric. /sarc From SkyNews - Wind-Powered Car Could Cut Chinas…
Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...
The DNA polymerase genes of human cytomegalovirus (HCMV) and varicella-zoster virus (VZV) were inserted separately into the polyhedrin gene of Autographa californica nuclear polyhedrosis virus (AcNPV) by cotransfection of Spodoptera frugiperda (SF9) cells with baculovirus transfer vectors carrying the genes and AcNPV infectious DNA. Infection of SF9 cells with the recombinant viruses resulted in expression from the polyhedrin promoter of proteins of the expected Mrs. These proteins possessed DNA polymerase activities similar to that of the enzymes induced by the respective herpesvirus in infected cells, and were identified as HCMV and VZV DNA polymerase using inhibitors and specific antisera reactive with each enzyme.
PEARCE, J. M., REEVES, A. B., RAMEY, A. M., HUPP, J. W., IP, H. S., BERTRAM, M., PETRULA, M. J., SCOTTON, B. D., TRUST, K. A., MEIXELL, B. W. and RUNSTADLER, J. A. (2011), Interspecific exchange of avian influenza virus genes in Alaska: the influence of trans-hemispheric migratory tendency and breeding ground sympatry. Molecular Ecology, 20: 1015-1025. doi: 10.1111/j.1365-294X.2010.04908.x ...
Virions of Moloney murine leukemia virus can synthesize two classes of DNA molecules complementary to their 70S RNA. One class consists of molecules about 200 nucleotides long, which are of limited sequence complexity; these molecules are formed preferentially if the dNTP concentration during the reaction is low. The second class consists of very heterogeneous DNA molecules with weight-average size of about 1,000 nucleotides containing at least 70% of the viral RNA sequences in approximately equal concentration. The longest of these molecules can be 5,000 nucleotides long. This second class of DNA is formed in large amounts only in reactions containing dNTP concentrations of 0.2 mM or higher. In such reactions after 24 h of incubation, at least 35% of the input RNA is represented in DNA copies. The ability to make long, representative DNA transcripts of tumor virus RNA provides a source of excellent probes for molecular hybridization. ...
To delineate the function of adenovirus early region 4 (E4) gene products, we constructed a set of mutant viruses which carry defined lesions within this coding region. Deletion and insertion mutations within six of seven known E4 coding regions had no measurable effect on virus growth in cultured cells. A variant carrying a deletion within the last coding region (encoding a 34,000-molecular-weight polypeptide) was modestly defective, and a mutant lacking the majority of the E4 region was severely defective for growth. The phenotypes of the two defective mutants are similar and complex. Both display perturbations in DNA replication, translation of the E2A mRNA, accumulation of late viral mRNAs, and host cell shutoff. ...
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The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
Negatively stained purified virions of parental virus strains and hybrid progenies. CPXV-NOHI (A), MVA-HANP (B), Rec 1 (C), Rec 2 (D), Rec 3 (E), Rec 3a (F), Re
Phenotypic change from transformed to normal induced by benzoquinonoid ansamycins accompanies inactivation of p60src in rat kidney cells infected with Rous sarc
Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors. ...
Wright, Edward, Bain, Mark, Teague, Linda, Murphy, Jane and Sinclair, John (2005) Ets-2 repressor factor recruits histone deacetylase to silence human cytomegalovirus immediate-early gene expression in non-permissive cells. Journal of General Virology, 86 (3). pp. 535-544. ISSN 0022-1317 ...
How do miRNAs pair with their target messages? miRNAs cause the translational repression or cleavage of target messages (Doench and Sharp 2004). Some miRNAs may behave like small interfering RNAs (siRNAs) that direct mRNA cleavage between the nucleotide positions 10 and 11 in the siRNA:mRNA target duplex (Tuschl et al. 1999; Zamore et al. 2000; Elbashir et al. 2001; Hutvágner and Zamore 2002a; Llave et al. 2002b; Martinez et al. 2002; Bartel 2004; Yekta et al. 2004). It appears that the extent of base pairing between the small RNA and the mRNA determines the balance between cleavage and degradation (Hutvágner and Zamore 2002a). Recent examples of cleavage of target messages are, in mouse, mir-196 guiding cleavage of Hox-B8 transcripts (Yekta et al. 2004) and, in Epstein Barr virus, miR-BART2, a virus-encoded miRNA, guiding the cleavage of transcripts for virus DNA polymerase (gene BALF5) (Pfeffer et al. 2004). While cleavage of mRNA is a straightforward process, the details of the mechanism of ...
gag Gene Products: Proteins coded by the retroviral gag gene. The products are usually synthesized as protein precursors or POLYPROTEINS, which are then cleaved by viral proteases to yield the final products. Many of the final products are associated with the nucleoprotein core of the virion. gag is short for group-specific antigen.
The 293 cell line was derived from primary cultures of human embryonic kidney (HEK) cells with sheared fragments of adenovirus (Ad) 5 DNA (Graham et al. 1977). HEK 293 cells contain the nucleotides 1-4344 of Ad5 which are located within the pregnancy-specific ß-1-glycoprotein 4 (PSG 4) gene. The transforming region of the human adenovirus contains the early region (E1), comprising two transcription units, E1a and E1b, whose products are essential and sufficient for mammalian cell transformation by adenoviruses (Louis et al. 1997). Because 293 cells express E1 gene products they are extensively used for the production of E1-deleted Ad viruses. Adeno-associated viruses (AAVs) belong to the family of Parvoviridae, being one of the smallest single-stranded and non-enveloped DNA viruses. AAVss are replication-deficient and have required co-infection with a helper adeno- or herpes virus for productive infection. The AAV Helper-free system takes advantage of the identification of the specific ...
This give the virus specificity, meaning that it will only be able to infect a certain host range. Once the viral genome is injected into the cell, then for a DNA phage, bacterial cells RNAP begins transcribing RNAs from certain promoters. The first class of viral genes is intermediate-early genes. Following translation of these genes, the early viral genes are transcribed. These genes encode proteins which get virus ready for DNA replication.. After DNA replication, usually into hundreds of copies, the late viral RNAs are made and translated into proteins. These act as structural components, either for capsid formation or cleaving and packaging of DNA into capsid, or to form the receptor on the capsid surface.. Nucleocapsid:. ...
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Endogenous retroviruses wormed into the human genome eons ago. Today viral genes continue to produce a variety of mysterious proteins in the body ...
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Viral gene therapy[edit]. Viral gene therapy uses genetically engineered viral vectors to deliver a gene of interest to target ... Viral gene therapy[edit]. Currently, there are many viral gene therapy products in clinical trial phases. Listed below are the ... Viral gene therapy uses genetically engineered viral vectors to deliver therapeutic genes to cells with genetic malfunctions[10 ... viral vectors for gene therapy and viral immunotherapy. These branches utilize three different types of treatment methods: gene ...
Bennett J (2003). "Immune response following intraocular delivery of recombinant viral vectors". Gene Therapy. 10 (11): 977-982 ... Rolling, F. (2004). "Recombinant AAV-mediated gene transfer to the retina: gene therapy perspectives". Gene Therapy. 11 (S1): ... "Human Gene Therapy. 20 (9): 999-1004. doi:10.1089/hum.2009.086. PMC 2829287. PMID 19583479.. ... "Human Gene Therapy. 19 (10): 979-990. doi:10.1089/hum.2008.107. PMC 2940541. PMID 18774912.. ...
"Entrez Gene: v-myb myeloblastosis viral oncogene homolog (avian)".. *^ Vargova K, Curik N, Burda P, Basova P, Kulvait V, ... while another gene (Suppressor of Pericarp Pigmentation 1 or SPP1) acts as a suppressor.[14] The maize P gene encodes a Myb ... Myb proto-oncogene protein also known as transcriptional activator Myb is a protein that in humans is encoded by the MYB gene.[ ... Gene ontology. Molecular function. • RNA polymerase II core promoter proximal region sequence-specific DNA binding. • DNA ...
Viral transmission of host derived gene. Some viruses steal host genes and then incorporate them into their own viral genome, ... Antigenic rift: Recombination of viral gene. This again occurs when there are again two viral cells that infect the same host ... PfEMP1 is encoded by the diverse family of genes known as the var family of genes (approximately 60 genes in all). The ... As the viral cells go through replication they reassort and the genes of the two species get mixed up and make 256 new ...
... in the precore region of the viral genome. The HBV has four genes: S, P, C, and X. The S gene codes for the "major" envelope ... The C gene codes for HBeAg and HBcAg. The C gene has a precore and a core region. If translation is initiated at the precore ... The X gene codes for HBxAg. The product of the X gene is hepatitis B x antigen (HBxAg). It may be involved in carcinogenesis. ... The precore region is not necessary for viral replication. Precore mutants can replicate. They are readily detectable by HBV ...
Knopf CW (1998). "Evolution of viral DNA-dependent DNA polymerases". Virus Genes. 16 (1): 47-58. doi:10.1023/A:1007997609122. ... Morphopoietic genes clustered at one end of the genome, with head genes preceding tail genes. Lytic enzymes were probably coded ... This virus has two large open reading frames one encoding the capsid gene and the other the Rep gene. These are bidirectionally ... clusters of orthologous genes and reconstruction of viral genome evolution". Virol. J. 6 (1): 223. doi:10.1186/1743-422X-6-223 ...
The E genes are also transcribed before viral DNA replication, but are dependent on the IE gene products. After entering the ... AHV-1 has 67 genes in its genome, 65 of which are likely coding genes. Three of the genes have no homologs to other herpesvirus ... L genes are transcribed "after the synthesis of DNA and viral protein onset". Virion DNA maturation occurs as the nucleocapids ... Immediate early begins after infection and before viral DNA replication. During this phase IE genes are transcribed without ...
Transcription of early genes 5. Replication of the viral genome Viral protein A cleaves replicative form I DNA strand at the ... Gene E is encoded with gene D with a +1 frameshift. Gene K overlaps genes A, B, and C. The origin of replication lies within a ... 6. Late genes are now transcribed by the host's RNA polymerase. 7. Synthesis of the new virons Viral protein C binds to ... It encodes 11 genes (in order: A, A*, B, C, K, D, E, J, F, G, and H), nine of which are essential. The nonessential genes are E ...
"Regulation of apoptosis by viral gene products". J. Virol. 71 (3): 1739-46. PMC 191242 . PMID 9032302.. CS1 maint: Uses authors ... This assay is used to disrupt gene function by creating an intragenic gene fusion. When an APAF-1 gene trap is introduced into ... Viral infection[edit]. Viral induction of apoptosis occurs when one or several cells of a living organism are infected with a ... The genes were identified by studies in the nematode C. elegans and homologues of these genes function in humans to regulate ...
These genes were passed on to the new virus. Gene sequences for every viral gene were made available through the Global ... Gene sequence information from Influenza Research Database Graphical Image of the viral makeup of the 2009 pandemic h1n1 virus ... and has carried an avian PB2 gene for at least ten years and a human PB1 gene since 1993. ... Analysis showed they were infected with the same strain - an H1N1 type containing genes of human, bird and swine origin. A 2004 ...
Wasps perhaps use microRNA to control the viral genes they carry.. *PolyDNAvirus can also use PTGS to interfere with the host's ... thanks to its gene PTP-H2.[10] It also decreases the adhesion capacity of hematocytes, thanks to its gene Glc1.8. The gene also ... The virus and wasp are in a mutualistic symbiotic relationship: expression of viral genes prevents the wasp's host's immune ... They promote viral RNA destruction. MicroRNA attach to viral-RNA because they are complementary. Then the complex is recognised ...
In acutely transforming viruses, the viral particles carry a gene that encodes for an overactive oncogene called viral-oncogene ... In either case, expression of these genes promotes the malignant phenotype of cancer cells. Tumor suppressor genes are genes ... Tumor suppressor genes[edit]. Many tumor suppressor genes effect signal transduction pathways that regulate apoptosis, also ... Viral[edit]. Main article: Oncovirus. Furthermore, many cancers originate from a viral infection; this is especially true in ...
Sariyer IK, Sariyer R, Otte J, Gordon J (2016). "Pur-alpha induces JCV gene expression and viral replication by suppressing ... which specifically bound a purine-rich sequence in the control region of the c-MYC gene, the gene, PURA, encoding the protein, ... PURB, the gene encoding Pur-beta, is located at 7p13. A visual fluorescence analysis of chromosomes from MDS patients shows ... Pur-alpha is a protein that in humans is encoded by the PURA gene located at chromosome 5, band q31. Pur-alpha an ancient, ...
As D protein is the most abundant gene transcript, it is the most protein in the viral procapsid. Similarly, gene transcripts ... only 8 are essential to viral morphogenesis. The GC-content is 44% and 95% of nucleotides belong to coding genes. ... that de novo H protein was required for optimal synthesis of other viral proteins. Mutations in H protein that prevent viral ... H protein (or the DNA Pilot Protein) pilots the viral genome through the bacterial membrane of E.coli bacteria (Jazwinski et al ...
... a novel Golgi-localized protein upregulated by viral infection". Gene. 249 (1-2): 53-65. doi:10.1016/S0378-1119(00)00136-0. ... "Entrez Gene: GOLPH2 golgi phosphoprotein 2". Laxman B, Morris DS, Yu J, et al. (February 2008). "A first-generation multiplex ... The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough ... The expression of this encoded protein has been observed to be upregulated in response to viral infection. Golgi membrane ...
Gene therapy. 15 (12): 911-20. doi:10.1038/gt.2008.21. PMID 18323793. Thirukkumaran, C; Morris, DG (2009). "Oncolytic viral ... selective viral replication in permissive cancer cells leading to tumor cell lysis; Innate immune response - viral replication ... Strong, JE; Coffey, MC; Tang, D; Sabinin, P; Lee, PW (1998). "The molecular basis of viral oncolysis: usurpation of the Ras ... and viral-associated antigens (VAA) to educate T-cells to recognize and destroy cancer cells. REOLYSIN has been evaluated in ...
October 2006). "Magnetic resonance imaging of viral particle biodistribution in vivo". Gene Therapy. 13 (20): 1440-46. doi: ... In gene therapy, gene delivery vectors, such as viruses, can be imaged according either to their particle biodistribution or ... such as MRI or SPECT/PET and latter means visualising the marker gene of gene delivery vector to be visible by the means of ...
Once the viral genes have been replicated, the procapsid is assembled and packed. The tail is then assembled and the mature ... and degrades the cell wall using viral exolysin enough to eject the viral DNA into the host cytoplasm via long flexible tail ... "Viral Zone". ExPASy. Retrieved 11 March 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 11 March 2015. NCBI. "T5likevirus ...
Early phase: early genes are transcribed in the cytoplasm by viral RNA polymerase. Early expression begins at 30 minutes post- ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the viral proteins to host ... Late phase: Late genes are expressed from 140 min to 48 hours post-infection, producing all structural proteins. Assembly of ... Intermediate phase: Intermediate genes are expressed, triggering genomic DNA replication at approximately 100 minutes post- ...
Once the viral genes have been replicated, the procapsid is assembled and packed. The tail is then assembled and the mature ... The virus attaches to the host cell's adhesion receptors using its terminal fiber, and ejects the viral DNA into the host ... "Viral Zone". ExPASy. Retrieved 15 June 2015. ICTV. "Virus Taxonomy: 2014 Release". Retrieved 15 June 2015. NCBI (February 2015 ... The complete genome is available here Viral replication is cytoplasmic. ...
Early phase: early genes are transcribed in the cytoplasm by viral RNA polymerase. Early expression begins at 30 minutes post- ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the viral proteins to host ... Late phase: Late genes are expressed from 140 min to 48 hours post-infection, producing all structural proteins. Assembly of ... "Viral Zone". ExPASy. Retrieved 15 June 2015. Viralzone: Parapoxvirus ICTV Virus Pathogen Database and Analysis Resource (ViPR ...
HSV latency is static; no virus is produced; and is controlled by a number of viral genes, including latency-associated ... Infectious skin disease: Viral cutaneous conditions, including viral exanthema (B00-B09, 050-059) ... HSV-1 has been proposed as a possible cause of Alzheimer's disease.[26][27] In the presence of a certain gene variation (APOE- ... Herpes simplex is a viral infection caused by the herpes simplex virus.[1] Infections are categorized based on the part of the ...
Early phase: early genes are transcribed in the cytoplasm by viral RNA polymerase. Early expression begins at 30 minutes post- ... Viral replication is cytoplasmic. Entry into the host cell is achieved by attachment of the viral proteins to host ... Late phase: Late genes are expressed from 140 min to 48 hours post-infection, producing all structural proteins. Assembly of ... The virus exits the host cell by microtubular outwards viral transport, and existing in occlusion bodies after cell death and ...
In these cells the viral genes that encode envelope proteins have restricted expression.[4] As a result, infectious particles ... Should the viral progression be diagnosed during stage 1 (even during late stage 1 when stage 2 symptoms start to manifest ...
"Entrez Gene: RALA v-ral simian leukemia viral oncogene homolog A (ras related)". Simicek M, Lievens S, Laga M, Guzenko D, ... identification of a novel mammalian Sec3 gene and a second Sec15 gene". The Journal of Biological Chemistry. 276 (32): 29792-7 ... Ras-related protein Ral-A (RalA) is a protein that in humans is encoded by the RALA gene on chromosome 7. This protein is one ... Polakis PG, Weber RF, Nevins B, Didsbury JR, Evans T, Snyderman R (Oct 1989). "Identification of the ral and rac1 gene products ...
Xin-Cheng Qin et al.: A tick-borne segmented RNA virus contains genome segments derived from unsegmented viral ancestors, in: ... Familie Geminiviridae (ssDNA(+/-): die einzelnen Gene haben unterschiedliche Polarität). *Genus Becurtovirus. *Genus ... Henxia Xia et al.: A dsRNA virus with filamentous viral particled, in: Nature Communicationsvolume 8, Nr. 168 (2017), [[doi: ...
a possible theory is that they are the resylt of horizontal gene transfer from a viral infection (intronless, 2 genes for one ... recombinase genes and viral transfer. Ralph M Bernstein ralph at ccit.arizona.edu Wed Mar 1 10:27:52 EST 1995 *Previous message ... question: can anyone support the viral introduction theory? can anyone give me an example of any other system where 2 genes ... these genes show no homology to eachother (or anyother genes for that matter) but are conserved for over 500 million years of ...
Our own treacherous immune genes can cause canc... Our own treacherous immune genes can cause cancer after viral infection. 5 ... Many of the mutations that cause this virally-induced cancer are caused by a family of genes that normally combats viral ... "Genes from the APOBEC family encode proteins that modify the DNA of invading viruses, causing mutations that prevent the virus ... "It is not clear why HPV infection causes the APOBEC genes to misbehave and mutate PIK3CA. It could be that the body responds to ...
As new antiviral ISGs continue to be identified and characterized, their roles in viral pathogenesis are also being explored in ... Our current understanding of how ISGs impact viral pathogenesis comes largely … ... Interferon-stimulated genes (ISGs) are critical for controlling virus infections. ... Interferon-stimulated genes: roles in viral pathogenesis Curr Opin Virol. 2014 Jun;6:40-6. doi: 10.1016/j.coviro.2014.03.006. ...
... all three polymerase genes (PB2, PB1, and PA), or all polymerase genes and the NP gene from Mem/88, with the rest derived from ... Human influenza a viral genes responsible for the restriction of its replication in duck intestine. *Hatta M ... These results indicate incompatibility between the genes of avian and human influenza A viruses and indicate that all genes ... the contribution of the other viral genes remains unknown. To determine the genetic basis for host range restriction of the ...
Researchers have identified signatures of viral infection, a distinction that may help doctors tell whether bacteria or a virus ... Gene behavior distinguishes viral from bacterial infections. New approach could gauge response to flu vaccine. ... CASING A CULPRIT Certain gene behavior changes in people can reveal whether an infection is caused by a virus, such as the H1N1 ... To find the viral fingerprints, computational immunologist Purvesh Khatri of Stanford University and colleagues combed through ...
1. Why is that viral gene in there? When you insert a new gene (such as an herbicide resistance gene in Monsantos Roundup ... Hidden Viral Gene Discovered In GMO Crops 391 Posted by Soulskill on Tuesday January 22, 2013 @07:13PM. from the food-is-tasty- ... It is unknown if the presence of the hidden viral genes were the result of laboratory contamination or a possible recombinant ... 3. How come the American scientists never detected this viral gene? * 3(a). Was it because of incompetence, or was it because ...
The notion that we may have viral genes in our genome isnt really all that odd. As Ryan points out in his introduction, when ... And were also built of viral genes, a product of the evolution of life, of the co-evolution of life and that strange creature ... Today were stardust and viral genes, an of mix or macro and micro. Being human has never been so interesting. And for readers ... We are Stardust… and Viral Genes. This post has already been read 4782 times! ...
The identification of several putative viral gene products including a DNA ligase and a viral antibiotic peptide is a powerful ... many attempts were made to elucidate the viral genome structure and the amino acid sequences of different viral gene products. ... Molecular Anatomy of Chilo Iridescent Virus Genome and the Evolution of Viral Genes. ... Handermann M., Schnitzler P., Rösen-Wolff A., Raab K., Sonntag K.-C., and Darai G., Virus Genes 6, 19-32, 1992.PubMedGoogle ...
Generating MHC class I ligands from viral gene products.. Yewdell J1, Antón LC, Bacik I, Schubert U, Snyder HL, Bennink JR. ... Here we review the efforts of our laboratory to understand how cells generate such peptides from viral gene products. We ... Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland 20892-0440, USA. ...
Furthermore, gene silencing generating a reversible effect has become an interesting alternative, and is well-suited for ... Viral vectors have been employed for the treatment of various diseases such as metabolic, cardiovascular, muscular, hematologic ... Significant improvements in vector engineering, delivery, and safety have placed viral vector-based therapy at the forefront of ... Several viral vector-based drugs have also been globally approved. ...
We are a world leader in novel AAV discovery, vector development, production, preclinical and clinical gene therapy research. ... AAV is the primary gene therapy platform driving the Horae Gene Therapy Center. ... What Are Gene Therapy Vectors?. Gene therapy vectors can be categorized into two classes: Artificial non-viral vectors and ... High-capacity AdV vectors are the exception as they do not carry any viral genes and have been shown to mediate long-term gene ...
... complicating the effectiveness of vaccines and anti-viral drugs employed in treating it. ... Host Genes Aiming at Anti-Viral Drugs and Vaccines Identified. by Nancy Needhima on January 30, 2012 at 10:57 PM General Health ... drugs that target host genes work more effectively because host genes rarely change or mutate. "If we target a host gene, the ... "We have the technology today that allows us to target specific genes in human cells and silence those genes to inhibit the ...
Viral small interfering RNAs target host genes to mediate disease symptoms in plants.. Smith NA1, Eamens AL, Wang MB. ... Viral Small Interfering RNAs Target Host Genes to Mediate Disease Symptoms in Plants ... Viral Small Interfering RNAs Target Host Genes to Mediate Disease Symptoms in Plants ... Viral Small Interfering RNAs Target Host Genes to Mediate Disease Symptoms in Plants ...
... improved lentiviral vector for use in gene therapy for sickle cell disease. ... Gene Expression, Gene Therapy, Gene Transfer, Gene-Editing, Genes, Genetic, Heart, Hemoglobin, I Cell Disease, in vitro, in ... In gene therapy approaches, researchers use viral vectors to deliver therapeutic genes into host bone marrow stem cells that ... Novel viral vector improves gene therapy for sickle cell disease. *Download PDF Copy ...
A blood-based gene expression assay was successfully used to identify viral respiratory disease in patients presenting to ... "Genes selected from the acute respiratory factor comprise the discriminant genes in the assay. Using a smaller set of genes ... A blood-based gene expression assay was successfully used to identify viral respiratory disease in patients presenting to ... Source Reference: Zaas, AK "A host-based RT-PCR gene expression signature to identify acute respiratory viral infection"Sci ...
... general Bacteriophages Genetic aspects Biologists Research Pregnancy proteins Viral genetics ... Do captured viral genes make human pregnancies possible? by Science News; Science and technology, ... In some cases, the viral genes still produce retroviruses. In other cases, a single gene, often the one encoding the protein ... genes+make+human+pregnancies+possible%3f-a062791146. *APA style: Do captured viral genes make human pregnancies possible?. (n.d ...
... today announced that its Sleeping Beauty non-viral gene transfer technology was featured in a perspectives article in the ... The Sleeping Beauty transposon-transposase is a unique non-viral system for introducing genes encoding T-cell receptors and ... ZIOPHARM Announces Sleeping Beauty Non-Viral Gene Transfer Technology Featured in Nature Medicine. ... today announced that its Sleeping Beauty non-viral gene transfer technology was featured in a perspectives article in the ...
A cows KISS: Calf resembling Gene Simmons goes viral. KERRVILLE, Texas -- Moo-ve over Gene Simmons. ... Gene Simmons (@genesimmons) July 31, 2017. "After a while, she just sat down in the shade like whatever, Im famous," Taylor ... KERRVILLE, Texas -- Moo-ve over Gene Simmons.. Only four days old and shes already become an Internet sensation. Meet Genie ... This is real, folks!!! Calf called Genie is born on Texas ranch and looks EXACTLY like Kiss rocker Gene Simmons https://t.co/ ...
... download and read Non-viral Gene Therapy ebook online in PDF format for iPhone, iPad, Android, Computer and Mobile readers. ... Non-viral Gene Therapy. Gene Design and Delivery. by Kazunari Taira(ed.) ; Kazunori Kataoka(ed.) ; Takuro Niidome(ed.) ... Title: Non-viral Gene Therapy. Author: Kazunari Taira; Kazunori Kataoka; Takuro Niidome. ... 1 Molecules for Gene Delivery 1-1 Leaf Huang Recent Progress in Nonviral Gene Delivery 1-2 Kenichi Yoshikawa Physical Chemistry ...
... David J Fink, University of Michigan School of Medicine, Ann Arbor, Michigan, USA ... In vivo gene therapy for pyridoxine‐induced neuropathy by HSV‐mediated gene transfer of neurotrophin‐3. Annals of Neurology 51 ... Fink, David J, and Glorioso, Joseph C(Jul 2007) Herpes Simplex Viral Vectors in Gene Therapy. In: eLS. John Wiley & Sons Ltd, ... Gene Therapy 12: 891-901. Glorioso JC and Fink DJ (2004) Herpes vector‐mediated gene transfer in treatment of diseases of the ...
Prevalent Eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection. Honglei ... Prevalent Eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection ... Prevalent Eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection ... Prevalent Eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection ...
Gene-altering technology aimed at preventing deadly diseases such as cancer from entering the DNA chain may not be as accurate ... CRISPR gene-editing tool to prevent cancer can spark deadly mutations - study Published time: 30 May, 2017 16:43 Edited time: ... Gene-altering technology aimed at preventing deadly diseases such as cancer from entering the DNA chain may not be as accurate ... Scientists restore hearing in deaf mice using advanced gene therapy However, a new study raises questions over the safety of ...
Gene Therapy Success Depends On Ability To Advance Viral Delivery Vectors To Commercialization. by Sam Savage ... Many gene therapy strategies designed to deliver a normal copy of a gene to cells carrying a disease-causing genetic mutation ... One technology platform that is well suited for in vivo delivery of genes is based on adeno-associated viruses (AAV). As these ... "Clinical proof of concept of AAV gene therapy has been realized in several diseases. The focus is now on issues related to ...
Unraveling cellular gene expression regulation during viral infection. Viruses are by definition fully dependent on the ... Although ribosome profiling provides precise and quantitative analysis of genes that are translationally regulated, our ... and the extent to which translation machinery contributes to the overall pattern of viral replication and pathogenesis remained ... we uncover diverse and dynamic translational regulation for subsets of host genes. ...
... *Download PDF Copy ... The study found that those with PDN who received two low dose rounds of a non-viral gene therapy called VM202 had significant ... Tags: Amputations, Blood, Blood Vessels, Cell, Cell Biology, Diabetes, Diabetic Neuropathy, Drugs, Foot, Foot Pain, Gene, Gene ... VM202 contains human hepatocyte growth factor (HGF) gene. Growth factor is a naturally occurring protein in the body that acts ...
  • The NIH is working to expedite the development of gene therapies, including gene editing, through its Cure Sickle Cell Initiative , which is part of the organization's larger, multi-pronged approach to reducing the burden of blood disorders. (news-medical.net)
  • ZIOPHARM Oncology is a Boston, Massachusetts-based biotechnology company employing novel gene expression, control and cell technologies to deliver safe, effective and scalable cell- and viral-based therapies for the treatment of cancer. (cnbc.com)
  • As these novel therapies move closer to commercialization, so do the methods for large-scale production and efficient delivery of AAV vectors, which are documented in a series of articles published online ahead of print in Human Gene Therapy, a peer-reviewed journal published by Mary Ann Liebert, Inc. ( www.liebertpub.com ). (redorbit.com)
  • Researchers have been trying for more than 20 years to harness this power in order to deliver beneficial gene therapies to patients. (hawaii.edu)
  • Viral vector-mediated gene therapies have been used in clinical trials for human diseases. (jimmunol.org)
  • This book provides a comprehensive overview of the pertinent molecular discoveries in the cancer field and explains how these are being used for gene-based cancer therapies. (abebooks.co.uk)
  • Focusing on speeding the process in clinical cancer care by bringing therapies as quickly as possible from bench to bedside, Cancer Gene Therapy by Viral and Non-viral Vectors is an absolutely vital book for physicians, clinicians, researchers, and students involved in this area of medicine. (abebooks.co.uk)
  • Several impressive successes in viral vector-based gene therapies have been reported in humans, including restoration of vision in patients with Leber's congenital amaurosis by retinal gene transfer and cures for severe immune deficiencies by gene transfer to hematopoietic stem cells. (frontiersin.org)
  • The current thesis has bridged the gap between non-viral gene delivery, stem cell therapy, and tissue engineering, which now presents new opportunities for further investigation utilizing non-viral gene delivery in concert with stem cell therapies for regenerative medicine applications. (ku.edu)
  • Clinical use of gene therapies to treat formerly incurable genetic diseases is advancing rapidly. (pall.com)
  • Many gene therapies have been developed using adherent cells in 2-dimensional flatware or roller bottles but using these technologies to reach commercial-scale production represents a significant challenge. (pall.com)
  • The organisation is in a strong position to support development of AAV therapies and has built an excellent knowledge base from which other viral vector and vaccine processes can be supported. (uk-cpi.com)
  • Several murine and canine models of dystrophinopathy have been used to study these diseases and evaluate potential therapies, including several viral gene therapy approaches. (omicsonline.org)
  • Drug Development & Delivery recently interviewed Dave Backer, Head of Virus & Gene Therapy Strategic Initiatives at MilliporeSigma, to discuss its expanding GMP capacity to speed development and manufacture of gene therapies, immunotherapies, and viral vaccines. (drug-dev.com)
  • The clinical manufacturing of intermediates and final products used in viral vaccines and gene therapies requires dedicated facilities, and there is significant demand for these services in the growing cell and gene therapy market. (drug-dev.com)
  • Q: What are the biggest challenges facing development and manufacturing of gene therapies and immunotherapies? (drug-dev.com)
  • NSCs are the subject of intense investigation, owing to their crucial roles in neural development and adult brain function and because they present potential targets for gene and cell replacement therapies following injury or disease. (biologists.org)
  • In parallel, the emergence of adeno-associated virus (AAV) as a clinically important vector raises the possibility of integrating these two technologies towards the development of gene editing therapies. (berkeley.edu)
  • CHAPEL HILL, NC - Gene therapies promise to revolutionize the treatment of many diseases, including neurological diseases such as ALS. (unchealthcare.org)
  • UNC School of Medicine researchers have now found a structure on these viruses that makes them better at crossing from the bloodstream into the brain - a key factor for administering gene therapies at lower doses for treating brain and spinal disorders. (unchealthcare.org)
  • This structural 'footprint' we found seems to help these viruses get efficiently into the brain, which informs the design of potentially safer brain-targeted gene therapies," said study senior author Aravind Asokan, PhD, associate professor in the department of genetics and the department of biochemistry and biophysics. (unchealthcare.org)
  • The study, published in Molecular Therapy , examined adeno-associated viruses (AAVs), the most commonly used virus vectors for delivering gene therapies. (unchealthcare.org)
  • This could be particularly useful for some gene therapies that target the brain. (unchealthcare.org)
  • Gene therapies against neurological diseases are under investigation in clinical and preclinical trials, and include therapies for ALS, Huntington's disease, Spinal Muscular Atrophy, Friedrich's ataxia, and other disorders. (unchealthcare.org)
  • Through its Cure Sickle Cell Initiative, NIH is working to accelerate the development of these and other new genetic therapies, including gene editing, with the goal of finding a cure for the disease. (mlo-online.com)
  • A knot polymer, poly[bis(2-acryloyl)oxyethyl disulphide- co -2-(dimethylamino) ethyl methacrylate] (DSP), was synthesized, optimized and evaluated as a non-viral vector for gene transfection for skin cells, keratinocytes. (rsc.org)
  • Conclusions- The new simplified approach for murine whole-heart viral transfection should assist molecular physiology studies. (ahajournals.org)
  • This study establishes the base for the application of these non-viral transfection systems for future treatments of retinal disorders. (arvojournals.org)
  • However, non-viral vectors circumvent the safety issues associated with viral vectors, but commonly exhibit low transfection efficiencies. (ku.edu)
  • A reporter gene such as enhanced green fluorescent protein facilitates monitoring spatial distribution of the vector, and the efficacy and cellular specificity of the transfection. (jove.com)
  • Immunoblots comparing activation of lytic cycle genes following the transfection of Rta and ZEBRA into Raji cells. (asm.org)
  • Total RNA prepared 30 h following transfection was analyzed by Northern blotting using probes for the indicated genes (see Materials and Methods). (asm.org)
  • Using confocal microscopy and fluorescent spectroscopy, the UB scientists tracked optically in real-time the process known as transfection, including the delivery of genes into cells, the uptake of genes by the nucleus and their expression. (buffalo.edu)
  • Researchers at the National Institutes of Health (NIH) have developed a unique, improved viral vector to use in gene therapy for sickle cell disease. (news-medical.net)
  • For the last three decades, researchers have been designing these vectors in a reverse structural orientation, which means the corrective genes that are introduced into the vector are translated from right to left (or backwards). (news-medical.net)
  • A blood-based gene expression assay was successfully used to identify viral respiratory disease in patients presenting to hospital emergency departments with fever of unknown origin, researchers reported. (medpagetoday.com)
  • The broad viral sensitivity of this assay may be a distinct advantage over pathogen-based antigen or PC testing should a new influenza virus, or potentially other viral type, begin to circulate in the community," the researchers wrote. (medpagetoday.com)
  • The new study represented the "proof of concept" that host expression of a relatively small set of genes, as measured by RT-PCR from blood RNA, can be a useful tool for identifying viral respiratory illness in the emergency department setting, the researchers wrote. (medpagetoday.com)
  • Many genes in the RT-PCR classifier have low regression coefficients, suggesting that it may be possible to significantly reduce the number of genes represented in the classifier and achieve similar results," the researchers wrote. (medpagetoday.com)
  • In 2014 , the process, which uses a Cas9 enzyme and ribonucleic acid to slice and bind sections of DNA, was shown capable of performing "targeted" gene deletion by researchers from the Fudan University Shanghai Cancer Center . (rt.com)
  • While researchers observed that the genes causing blindness had been repaired, some 1,500 accidental mutations were also found in the test subjects. (rt.com)
  • The technique may help researchers decide which mutated genes are most deserving of further study, and help pharmaceutical firms develop drugs with better chances of success. (healthcanal.com)
  • The researchers found complete inhibition of tumor growth in the mice that were treated immediately with gene therapy and significant tumor inhibition in the 30- and 60-day delayed treatment mice. (fightaging.org)
  • The animals are otherwise healthy and the change - introduced using gene-editing technology - should not affect their ability to fight off other infections, the researchers say. (ed.ac.uk)
  • In sum, researchers are now being more realistic than in the early days, seeing the future of gene therapy in providing effective treatments, not cures. (sigmaaldrich.com)
  • In advanced lab tests using animal models, the new vector was up to 10 times more efficient at incorporating corrective genes into bone marrow stem cells than the conventional vectors currently used, and it had a carrying capacity of up to six times higher, the researchers report. (nih.gov)
  • Gene therapy trials using reverse-oriented vectors for sickle cell disease and beta-thalassemia have largely been encouraging, the researchers said, but this complicated gene translation process has made vector preparation and gene-transfer efficiency more difficult. (nih.gov)
  • Now, researchers at Washington University School of Medicine in St. Louis report that they can distinguish between viral and bacterial infections in children with fever by profiling the activity of genes in a blood sample. (healthcanal.com)
  • Using microarray technology, researchers can distinguish between viral and bacterial infections in children with fever by profiling the activity of genes in a blood sample. (healthcanal.com)
  • Using microarray technology, the researchers could easily distinguish bacterial infections from viral infections based on distinctive patterns of gene expression. (healthcanal.com)
  • Because of the challenges of achieving this goal, the authors note that nonviral integration systems can be considerably cheaper to manufacture and easier to implement for single-use applications compared with viral vectors and that, among these, Sleeping Beauty has advanced farthest in clinical development. (cnbc.com)
  • This review outlines recent developments on the contributions of various ISGs to viral disease outcomes in vivo. (nih.gov)
  • However artificial vectors remain much less efficient than viral vectors for in vivo or ex vivo gene transfer and transgene expression is generally transient. (umassmed.edu)
  • However, in vivo administration of AdV triggers strong immune responses, which preclude their use for gene therapy applications in genetic diseases where long-term gene expression may be necessary. (umassmed.edu)
  • High-capacity AdV vectors are the exception as they do not carry any viral genes and have been shown to mediate long-term gene expression in vivo . (umassmed.edu)
  • Recombinant AAV (rAAV) vectors carrying inverted terminal repeats as the only viral component entered the gene therapy arena much later than retroviral and AdV vectors, but have quickly gained popularity due to their broad tissue tropism, exceptional in vivo gene transfer efficiency and sustained transgene expression, as well as a proven safety profile in humans. (umassmed.edu)
  • In vivo gene therapy for pyridoxine‐induced neuropathy by HSV‐mediated gene transfer of neurotrophin‐3. (els.net)
  • In "A Simple Method to Increase the Transduction Efficiency of Single-Stranded Adeno-Associated Virus Vectors In Vitro and In Vivo," Ma and colleagues have further optimized their method for delivering a mixed population of AAV2 vectors to enable high-efficiency transfer of large genes. (redorbit.com)
  • We contradict this view by demonstrating that decreased gene packaging can be associated with increased in vivo fitness and transmissibility. (pnas.org)
  • One of the advantages of using an AAV vector is the ability to extend the period of transgene expression both in vitro and in vivo compared with adenoviral vector-mediated gene expression. (jimmunol.org)
  • Background- Viral gene transfer to the whole heart in vivo has been achieved in several mammalian species but remained difficult to accomplish in murine hearts. (ahajournals.org)
  • The present study tested this methodology for both adenovirus (AdV)-mediated and adeno-associated virus (AAV)-mediated gene transfer to the in vivo murine heart. (ahajournals.org)
  • To provide proof of principal for stably altering in vivo cardiac function, we restored murine phospholamban (PLB) to mice lacking the PLB gene and provide novel insights regarding its role to the in vivo modulation of cardiac function by beat frequency. (ahajournals.org)
  • In this work, we have assessed the impact in vivo of the evasion gene A238L of African swine fever virus, an inhibitor of both NF-κB- and NFAT-mediated transcription. (springer.com)
  • To further investigate the 1-8U function with both in vivo and in vitro studies, the 1-8U gene was found to suppress cellular and HCV IRES-mediated translation. (wiley.com)
  • In this work two different peptidic constructions (R9-GFP-His and HNRK) have been tested on in-vitro and in-vivo models for its application in non-viral retinal gene therapy. (arvojournals.org)
  • In vivo - where a viral vector contains the gene of interest is injected into the patient, the virus targets a particular cell type, is infected by the virus and multiple copies of the protein are produced by the patient's cells. (uk-cpi.com)
  • The results presented in this ex vivo system may be a first step toward expressing genes with products that could be continuously delivered to the eye through the tears. (curehunter.com)
  • Identification of specific sites and patterns of HLA-associated polymorphisms across HIV protease, RT, Vpr, and Nef illuminates regions of the genes encoding these products under active immune selection pressure in vivo. (ubc.ca)
  • Unfortunately, most gene delivery vectors are incapable of efficient or specific gene delivery to NSCs in vivo . (biologists.org)
  • In vivo results indicate that co-expression of BMP2/7 applied by non-viral naked DNA gene transfer effectively mediates bone formation without the application of biomaterials, cells or recombinant growth factors, offering a promising alternative to current treatment strategies with potential for clinical translation in the future. (whiterose.ac.uk)
  • Many of the mutations that cause this virally-induced cancer are caused by a family of genes that normally combats viral infections, finds new UCL research. (ucl.ac.uk)
  • Previous studies have shown that APOBECs cause mutations in a range of cancers but our finding that they mutate key cancer genes implicates them as drivers of tumour development, particularly in HPV-associated cancers. (ucl.ac.uk)
  • Gene-altering technology aimed at preventing deadly diseases such as cancer from entering the DNA chain may not be as accurate as first thought, with a new study suggesting CRISPR-Cas9 editing risks accidental mutations. (rt.com)
  • Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
  • Mutations in the dystrophin gene predispose the myofiber membrane to contraction-induced membrane damage, resulting in inflammation, and myonecrosis, and progressive loss of functional muscle tissue. (omicsonline.org)
  • Another danger is that the new gene might be inserted in the wrong location in the DNA , possibly causing harmful mutations to the DNA or even cancer. (genetherapynet.com)
  • Genomic integration ensures transmission of the vector transgene cassette and its expression in the progeny, but its randomness carries a risk of insertional mutagenesis by potentially disrupting tumor suppressor genes or activating oncogenes. (umassmed.edu)
  • We are interested in the gene regulation of phages, complex ecological interactions, the genomic and functional trait diversity of phages in real populations, and their applicability as antibiotic alternatives. (gatech.edu)
  • Importantly, the capacity of these nucleases to modify specific genomic loci associated with human disease could render new classes of genetic disease, including autosomal dominant or even idiopathic disease, accessible to gene therapy. (berkeley.edu)
  • Genomic analysis of a Trichoplusia ni Betabaculovirus (TnGV) with three different viral enhancing factors and two unique genes. (nextbio.com)
  • Gene therapy approaches that use these reverse-oriented vectors for sickle cell disease have so far been encouraging, but Tisdale and team say this gene translation process has made vector preparation and the efficiency of gene transfer more challenging. (news-medical.net)
  • The Company's synthetic immuno-oncology programs, in collaboration with Intrexon Corporation (NYSE:XON) and the MD Anderson Cancer Center, include chimeric antigen receptor T cell (CAR-T) and other adoptive cell based approaches that use non-viral gene transfer methods for broad scalability. (cnbc.com)
  • It is the major obstacle to success of therapeutic approaches using genes and oli- nucleotides,including siRNAs. (ebooks.com)
  • Combinations of these approaches will likely facilitate clinical applications of gene therapy for many target diseases and also aid in vaccine development. (frontiersin.org)
  • Viral gene therapy approaches have also been investigated for other disorders of skeletal muscle, including lysosomal storage disorders and congenital myopathies, and these studies have benefitted immensely from the pioneering work that was performed using dystrophinopathy models. (omicsonline.org)
  • Advanced technologies of conditional gene manipulation in the mouse enable multifaceted approaches to the exploration of synaptic pathways and functional connections in the central nervous system. (jove.com)
  • Through a combination of rational design and directed evolution approaches, we attempt to expand traditional virus targeting methods to create novel AAV gene delivery vectors with enhanced specificity. (confex.com)
  • Alternatively, there may well be something about the virus that causes the APOBEC response to wrongly target the body's own genes for mutation. (ucl.ac.uk)
  • Sickle cell disease is an inherited blood disorder caused by a mutation in the beta-globin gene that results in hemoglobin having an abnormal structure. (news-medical.net)
  • The vector is modified to carry corrective genes that will have therapeutic effects, often by counteracting a genetic mutation. (news-medical.net)
  • Many gene therapy strategies designed to deliver a normal copy of a gene to cells carrying a disease-causing genetic mutation rely on a modified virus to transfer the gene product into affected tissues. (redorbit.com)
  • The abnormal gene could be repaired through selective reverse mutation, which returns the gene to its normal function. (scribd.com)
  • Control mice (C57/Bl6, 2 to 4 months) and littermate SVJ129/CF-1 mice with and without a null mutation for the PLB gene (PLB −/− , 6 to 8 months) were used. (ahajournals.org)
  • Gene therapy is the introduction of new genetic material into a cell as a means to correct a known mutation that causes a disease. (jyi.org)
  • Sickle cell disease is an inherited blood disorder caused by a mutation, or misspelling, in the beta-globin gene (or β-globin gene). (nih.gov)
  • The genetic mutation responsible for OTC occurs on the X chromosome, so women are typically carriers, while their sons with the mutated gene suffer the disease. (technologynetworks.com)
  • Antibody to v-fes gene product was found to occur before tumor development in group B and after tumor development in group A. v-Ses was found to be down-regulated and extensively mutated in tumors of group B. These results suggest viral gene mutation as a determinant of low grade malignancy of lesions induced. (spandidos-publications.com)
  • Entry via the endosome guarantees low pH and exposure to proteases which are needed to open the viral capsid and release the genetic material inside. (wikipedia.org)
  • The Sleeping Beauty transposon-transposase is a unique non-viral system for introducing genes encoding T-cell receptors and chimeric antigen receptors into lymphocytes that was exclusively licensed to ZIOPHARM and its collaboration partner, Intrexon Corporation (NYSE:XON), through an agreement with the MD Anderson Cancer Center. (cnbc.com)
  • Innate and antigen-specific adaptive immune responses against viral vectors and therapeutic transgene products pose serious hurdles for successful gene therapy. (frontiersin.org)
  • This gene quickly helps the virus to hide from the immune system by subverting normal antigen presentation to T cells, which have the critical task of destroying virally-infected cells," Belz says. (infectioncontroltoday.com)
  • Efficient and preferential gene delivery would offer a versatile and rapid means to study regulatory mechanisms of NSC quiescence, proliferation, self-renewal and differentiation. (biologists.org)
  • The recent discovery of a new class of regulatory genes known as microRNAs (miRNAs) has resulted in a paradigm shift in gene regulation research. (prolekare.cz)
  • Viral vectors have been employed for the treatment of various diseases such as metabolic, cardiovascular, muscular, hematologic, ophthalmologic, and infectious diseases and different types of cancer. (mdpi.com)
  • Clinical proof of concept of AAV gene therapy has been realized in several diseases. (redorbit.com)
  • After completing a medical degree and then a PhD, he carried out post-doctoral research on gene therapy for liver diseases at Necker Hospital in Paris. (indigo.ca)
  • Skeletal muscle disorders represent considerable opportunities for investigators developing viral gene therapy strategies, due to the numerous monogenetic diseases of skeletal muscle, the clear and quantifiable clinical phenotypes, and the easy accessibility of muscle tissue. (omicsonline.org)
  • In an effort to improve the efficacy (i.e., killing speed) of the LdMNPV, we generated a recombinant viral strain (vEGT-) that does not produce the enzyme ecdysteroid UDP-glucosyltransferase (EGT). (usda.gov)
  • Miskin JE, Abrams CC, Goatley LC, Dixon LK (1998) A viral mechanism for inhibition of the cellular phosphatase calcineurin (New York, NY). (springer.com)
  • Our new vector is an important breakthrough in the field of gene therapy for sickle cell disease," said study senior author John Tisdale, M.D., chief of the Cellular and Molecular Therapeutic Branch at the National Heart, Lung, and Blood Institute (NHLBI). (nih.gov)
  • Our studies demonstrate that a viral miRNA mediates translational repression of multiple cellular genes by targeting mRNA 5′UTRs. (prolekare.cz)
  • Our new vector is an important breakthrough in the field of gene therapy for sickle cell disease. (news-medical.net)
  • However, it has broad applications for correcting single gene disorders, such as hemophilia, sickle cell disease and muscular dystrophy. (hawaii.edu)
  • Diagram shows steps involved in conducting gene therapy for sickle cell disease. (nih.gov)
  • The development of the vector could make gene therapy for sickle cell disease much more effective and pave the way for wider use of it as a curative approach for the painful, life-threatening blood disorder. (nih.gov)
  • Already an estimated 27 people with sickle cell disease have undergone experimental gene therapy using conventional vectors. (mlo-online.com)
  • Our current understanding of how ISGs impact viral pathogenesis comes largely from studies in knockout mice, with isolated examples from human clinical data. (nih.gov)
  • This has simplified the gene translation process and when the new vector was tested in mice and monkeys, it delivered up to six times more therapeutic beta-globin genes than conventional vectors. (news-medical.net)
  • They treated some of the mice immediately with a genetically engineered vaccinia virus containing a gene coding cytosine deaminase, a suicide gene , and delayed treatment of other mice for 30 or 60 days. (fightaging.org)
  • Mice homozygous for a gene trap insertion exhibit retinal degeneration, and increased total body mass and total body fat. (jax.org)
  • Administration of IL-10, or IL-10 gene transfer, prevents autoimmune diabetes in nonobese diabetic (NOD) mice. (jimmunol.org)
  • In this study, we report suppression of autoimmune diabetes in NOD mice by viral IL-10 (vIL-10) gene therapy using an AAV vector. (jimmunol.org)
  • DENVER---University of Michigan scientists have developed a new generation of "gutted" viral vectors that deliver the gene for dystrophin to the muscles of adult mice with muscular dystrophy without triggering their immune systems to attack the foreign virus. (bio-medicine.org)
  • Using this new version of our viral vector, we have induced stable production of dystrophin for at least four months in muscle fibers of adult, dystrophic mice with normal immune systems," Salvatori said. (bio-medicine.org)
  • In contrast, more than 30 percent of the untreated OTC-deficient mice died after a week and their ammonia levels were significantly higher than the OTC mice whose genes were corrected. (technologynetworks.com)
  • Mice generated from these clones may be crossed with an expanding number of mouse lines that express DNA recombinases under promoters or loci specific to a particular population of neurons for selective gene manipulation ( http://nagy.mshri.on.ca/cre_new/index.php ). (jove.com)
  • Stereotaxic injection of a viral vector that expresses Cre or Flp allows limiting gene recombination to regions in the spinal cord of mice in which DNA fragments are flanked by loxP or Frt sites, so-called floxed or flrted alleles. (jove.com)
  • Unlike constitutive DNA rearrangement, which would result from crossbreeding the animals with recombinase expressing mice, this strategy also provides temporal control over gene activation or silencing. (jove.com)
  • When this gene is removed from the virus, the innate human immune system destroys HIV by mutating it to the point where it can no longer survive. (infectioncontroltoday.com)
  • Without the vif gene, HIV can be completely destroyed by the body's own immune system, says J. Victor Garcia, PhD, professor of medicine at the UNC School of Medicine and senior author on the study. (infectioncontroltoday.com)
  • A safe packaging line for gene transfer: separating viral genes on two different plasmids. (asm.org)
  • These results suggest that gag, pol, and env, when present on different plasmids, may provide an efficient and safe packaging line for use in retroviral gene transfer. (asm.org)
  • This suggests that the previously observed species specificity of Y-Sat-induced symptoms is due to natural sequence variation in the CHLI gene, preventing CHLI silencing in species with a mismatch to the Y-Sat siRNA. (nih.gov)
  • A modified retrovirus for gene therapy contains psi sequence, a gene of interest at a multiple cloning site (MCS), neomycin resistance gene with promoter or using an internal ribosome entry element (IRES). (scribd.com)
  • Three envelope glycoprotein genes (ORF 25, 65 and 116) of the CyHV-3 isolates from the USA, Israel, Japan and Korea were compared, and interestingly, sequence insertions or deletions were observed in these target regions. (mdpi.com)
  • Their analysis divided the genes into two sets: 847 with at least one 3'UTR matching sequence and 424 without a matching sequence. (cancer.gov)
  • In contrast to the old vector, the gene sequence, or "message," of the new beta-globin vector is read from left to right-like reading a normal sentence-making the gene translation approach less complicated, Tisdale explained. (nih.gov)
  • Despite the formidable mutational capacity and sequence diversity of HIV-1, evidence suggests that viral evolution in response to specific selective pressures follows generally predictable mutational pathways. (ubc.ca)
  • We applied a viral lineage-corrected analytical method to investigate HLA class I-associated sequence imprinting in HIV protease, reverse transcriptase (RT), Vpr, and Nef in a large cohort of chronically infected, antiretrovirally naïve individuals. (ubc.ca)
  • The increasing abundance of sequence data has exacerbated a long known problem: gene trees and species trees for the same terminal taxa are often incongruent. (biomedcentral.com)