Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A cell line derived from cultured tumor cells.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Established cell cultures that have the potential to propagate indefinitely.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Luciferases from FIREFLIES, usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
Nucleic acid sequences involved in regulating the expression of genes.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The sequence at the 3' end of messenger RNA that does not code for product. This region contains transcription and translation regulating sequences.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Luciferases from RENILLA that oxidizes certain LUMINESCENT AGENTS to cause emission of PHOTONS.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.
Recombinases that insert exogenous DNA into the host genome. Examples include proteins encoded by the POL GENE of RETROVIRIDAE and also by temperate BACTERIOPHAGES, the best known being BACTERIOPHAGE LAMBDA.
The region of DNA which borders the 5' end of a transcription unit and where a variety of regulatory sequences are located.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
ANIMALS whose GENOME has been altered by GENETIC ENGINEERING, or their offspring.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The sequence at the 5' end of the messenger RNA that does not code for product. This sequence contains the ribosome binding site and other transcription and translation regulating sequences.
Deletion of sequences of nucleic acids from the genetic material of an individual.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
Proteins found in any species of bacterium.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
A multiprotein complex composed of the products of c-jun and c-fos proto-oncogenes. These proteins must dimerize in order to bind to the AP-1 recognition site, also known as the TPA-responsive element (TRE). AP-1 controls both basal and inducible transcription of several genes.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Proteins prepared by recombinant DNA technology.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Sequences of DNA in the genes that are located between the EXONS. They are transcribed along with the exons but are removed from the primary gene transcript by RNA SPLICING to leave mature RNA. Some introns code for separate genes.
The integration of exogenous DNA into the genome of an organism at sites where its expression can be suitably controlled. This integration occurs as a result of homologous recombination.
Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.
Compound such as LUMINESCENT PROTEINS that cause or emit light (PHYSICAL LUMINESCENCE).
Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
Chromosomal, biochemical, intracellular, and other methods used in the study of genetics.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
The family Lampyidae, which are bioluminescent BEETLES. They contain FIREFLY LUCIFERIN and LUCIFERASES. Oxidation of firefly luciferin results in luminescence.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; FLUORESCENCE IMAGING; and MICROSCOPY.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC
Emission of LIGHT when ELECTRONS return to the electronic ground state from an excited state and lose the energy as PHOTONS. It is sometimes called cool light in contrast to INCANDESCENCE. LUMINESCENT MEASUREMENTS take advantage of this type of light emitted from LUMINESCENT AGENTS.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Transport proteins that carry specific substances in the blood or across cell membranes.
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
Elements of limited time intervals, contributing to particular results or situations.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The parts of a transcript of a split GENE remaining after the INTRONS are removed. They are spliced together to become a MESSENGER RNA or other functional RNA.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.

Transcriptional repression by the Drosophila giant protein: cis element positioning provides an alternative means of interpreting an effector gradient. (1/16045)

Early developmental patterning of the Drosophila embryo is driven by the activities of a diverse set of maternally and zygotically derived transcription factors, including repressors encoded by gap genes such as Kruppel, knirps, giant and the mesoderm-specific snail. The mechanism of repression by gap transcription factors is not well understood at a molecular level. Initial characterization of these transcription factors suggests that they act as short-range repressors, interfering with the activity of enhancer or promoter elements 50 to 100 bp away. To better understand the molecular mechanism of short-range repression, we have investigated the properties of the Giant gap protein. We tested the ability of endogenous Giant to repress when bound close to the transcriptional initiation site and found that Giant effectively represses a heterologous promoter when binding sites are located at -55 bp with respect to the start of transcription. Consistent with its role as a short-range repressor, as the binding sites are moved to more distal locations, repression is diminished. Rather than exhibiting a sharp 'step-function' drop-off in activity, however, repression is progressively restricted to areas of highest Giant concentration. Less than a two-fold difference in Giant protein concentration is sufficient to determine a change in transcriptional status of a target gene. This effect demonstrates that Giant protein gradients can be differentially interpreted by target promoters, depending on the exact location of the Giant binding sites within the gene. Thus, in addition to binding site affinity and number, cis element positioning within a promoter can affect the response of a gene to a repressor gradient. We also demonstrate that a chimeric Gal4-Giant protein lacking the basic/zipper domain can specifically repress reporter genes, suggesting that the Giant effector domain is an autonomous repression domain.  (+info)

Accelerated accumulation of somatic mutations in mice deficient in the nucleotide excision repair gene XPA. (2/16045)

Inheritable mutations in nucleotide excision repair (NER) genes cause cancer-prone human disorders, such as xeroderma pigmentosum, which are also characterized by symptoms of accelerated ageing. To study the impact of NER deficiency on mutation accumulation in vivo, mutant frequencies have been determined in liver and brain of 2-16 month old NER deficient XPA-/-, lacZ hybrid mice. While mutant frequencies in liver of 2-month old XPA-/-, lacZ mice were comparable to XPA+/-, lacZ and the lacZ parental strain animals, by 4 months of age mutant frequencies in the XPA-deficient mice were significantly increased by a factor of two and increased further until the age of 16 months. In brain, mutant frequencies were not found to increase with age. These results show that a deficiency in the NER gene XPA causes an accelerated accumulation of somatic mutations in liver but not in brain. This is in keeping with a higher incidence of spontaneous liver tumors reported earlier for XPA-/- mice after about 15 months of age.  (+info)

Inducible genetic suppression of neuronal excitability. (3/16045)

Graded, reversible suppression of neuronal excitability represents a logical goal of therapy for epilepsy and intractable pain. To achieve such suppression, we have developed the means to transfer "electrical silencing" genes into neurons with sensitive control of transgene expression. An ecdysone-inducible promoter drives the expression of inwardly rectifying potassium channels in polycistronic adenoviral vectors. Infection of superior cervical ganglion neurons did not affect normal electrical activity but suppressed excitability after the induction of gene expression. These experiments demonstrate the feasibility of controlled ion channel expression after somatic gene transfer into neurons and serve as the prototype for a novel generalizable approach to modulate excitability.  (+info)

An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast. (4/16045)

The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well.  (+info)

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo. (5/16045)

Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues 1-32 or 1-122) to green fluorescent protein (GFP) and analyzed the subcellular localization of these fusion proteins. We show that the first 32 amino acids of Gi1alpha are sufficient to target GFP to caveolin-enriched domains of the plasma membrane in vivo, as demonstrated by co-fractionation and co-immunoprecipitation with caveolin-1. Interestingly, when dual acylation of this 32-amino acid domain was blocked by specific point mutations (G2A or C3S), the resulting GFP fusion proteins were localized to the cytoplasm and excluded from caveolin-rich regions. The myristoylated but nonpalmitoylated (C3S) chimera only partially partitioned into caveolin-containing fractions. However, both nonacylated GFP fusions (G2A and C3S) no longer co-immunoprecipitated with caveolin-1. Taken together, these results indicate that lipid modification of the NH2-terminal of Gi1alpha is essential for targeting to its correct destination and interaction with caveolin-1. Also, a caveolin-1 mutant lacking all three palmitoylation sites (C133S, C143S, and C156S) was unable to co-immunoprecipitate these dually acylated GFP-G-protein fusions. Thus, dual acylation of the NH2-terminal domain of Gi1alpha and palmitoylation of caveolin-1 are both required to stabilize and perhaps regulate this reciprocal interaction at the plasma membrane in vivo. Our results provide the first demonstration of a functional role for caveolin-1 palmitoylation in its interaction with signaling molecules.  (+info)

Fibroblast growth factor-8 expression is regulated by intronic engrailed and Pbx1-binding sites. (6/16045)

Fibroblast growth factor-8 (FGF8) plays a critical role in vertebrate development and is expressed normally in temporally and spatially restricted regions of the vertebrate embryo. We now report on the identification of regions of Fgf8 important for its transcriptional regulation in murine ES cell-derived embryoid bodies. Stable transfection of ES cells, using a human growth hormone reporter gene, was employed to identify regions of the Fgf8 gene with promoter/enhancer activity. A 2-kilobase 5' region of Fgf8 was shown to contain promoter activity. A 0.8-kilobase fragment derived from the large intron of Fgf8 was found to enhance human growth hormone expressed from the Fgf8 promoter 3-4-fold in an orientation dependent manner. The intronic fragment contains DNA-binding sites for the AP2, Pbx1, and Engrailed transcription factors. Gel shift and Western blot experiments documented the presence of these transcription factors in nuclear extracts from ES cell embryoid bodies. In vitro mutagenesis of the Engrailed or Pbx1 site demonstrated that these sites modulate the activity of the intronic fragment. In addition, in vitro mutagenesis of both Engrailed and Pbx1 sites indicated that other unidentified sites are responsible for the transcriptional enhancement observed with the intronic fragment.  (+info)

Regulation of human hsp90alpha gene expression. (7/16045)

Mammalian HSP90alpha and HSP90beta are encoded by two individual genes. On the basis of the upstream sequences of the human hsp90alpha gene, GenBank accession number U25822, we have constructed CAT reporter plasmids driven by individual fragments of the hsp90alpha gene. We found that (1) the proximal heat shock element complex located at -96/-60 enhances hsp90alpha promoter expression; (2) heat shock induction depends upon the coexistence of distal heat shock element at -1031/-1022 and the proximal heat shock element complex of the hsp90alpha gene; (3) unlike hsp90beta, downstream sequences of the transcription start site inhibit hsp90alpha expression. We conclude that the regulatory mechanisms for the expression of hsp90alpha and hsp90beta genes are different.  (+info)

Regulatory sequences of the mouse villin gene that efficiently drive transgenic expression in immature and differentiated epithelial cells of small and large intestines. (8/16045)

Villin is an early marker of epithelial cells from the digestive and urogenital tracts. Indeed villin is expressed in the stem cells and the proliferative cells of the intestinal crypts. To investigate the underlying molecular mechanisms and particularly those responsible for the restricted tissue specificity, a large genomic region of the mouse villin gene has been analyzed. A 9-kilobase (kb) regulatory region of the mouse villin gene (harboring 3.5 kb upstream the transcription start site and 5.5 kb of the first intron) was able to promote transcription of the LacZ reporter gene in the small and large intestines of transgenic mice, in a transmissible manner, and thus efficiently directed subsequent beta-galactosidase expression in epithelial cells along the entire crypt-villus axis. In the kidney, the transgene was also expressed in the epithelial cells of the proximal tubules but is likely sensitive to the site of integration. A construct lacking the first intron restricted beta-galactosidase expression to the small intestine. Thus, the 9-kb genomic region contains the necessary cis-acting elements to recapitulate the tissue-specific expression pattern of the endogenous villin gene. Hence, these regulatory sequences can be used to target heterologous genes in immature and differentiated epithelial cells of the small and/or large intestinal mucosa.  (+info)

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TLR2/NF-kB reporter cell line/TLR2 cell line can be used to monitor the TLR2 signaling pathway activity. Screen for activators or inhibitors of the TLR signaling pathway.
Reporter genes are fused to DNA fragments from a test gene or promoter. Reporter genes must be easily measured enzymatically or visually. Two common reporter genes are Β-galactosidase, green fluorescent protein (GFP) and yellow fluorescent protein (YFP). Β-gal is measured enzymatically using either X-gal (which Β-gal cleaves into a blue compound) or ONPG (which Β-gal cleaves into a yellow compound). GFP and YFP can be measured enzymatically, but are more easily measured visually because they fluoresce brightly.. ...
TY - GEN. T1 - Measurement of (anti-)oestrogenic potency in complex mixtures using a novel stably transfected luciferase reporter gene assay in the human T47D breast cancer cell. AU - Legler, J.. AU - van den Brink, C.. AU - Brouwer, A.. AU - van der Saag, P.. AU - Vethaak, A.D.. AU - Murk, A.J.. AU - van der Burg, B.. PY - 2000. Y1 - 2000. M3 - Conference paper. SP - 104. EP - 108. BT - Endocrine Disrupting Compounds : Wildlife and human health risks, The Hague 1998. CY - The Hague. ER - ...
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Regulation of gene expression can be achieved by fusing miRNA binding sites into the 3UTR of a GOI. In case a referring shRNA miR is endogenously present or co-expressed, the GOI is knocked down. Strength of regulation thereby depends on binding site properties. We are able to tune gene expression linearly over a broad range. This is a first proof of principle for various miRNA-mRNA interaction in vitro. Therefore, we transfected HeLa cells in principle with our pSMB_miTuner Plasmid HD3 . It turned out, that no obvious effect of different binding sites on reporter gene expression could be measured (data not shown). We assume that the RSV promoter driving the shRNA miR is too weak for tight regulation of the referring binding site behind the GOI. Only if a sufficient amount of shRNA miR binds to its target, translation is significantly repressed. Thus, we expressed the shRNA miR from a separate plasmid which was always co-transfected with the original tuning construct. The reporter genes - i. e. ...
Regulation of gene expression can be achieved by fusing miRNA binding sites right behind a GOI. In case a referring shRNA miR is expressed, the GOI is knocked down. Strength of regulation thereby depends on binding site properties. We are able to tune gene expression linearly over a broad range. This is a first proof of principle for various miRNA-mRNA interaction in vitro. Therefore, we transfected HeLa cells in principle with our pSMB_miTuner Plasmid HD3. It turned out, that there was no obvious effect of different binding sites on reporter gene expression (data not shown). We assume that the RSV driving the shRNA miR is too weak for tight regulation of the referring binding site behind the GOI. Only if a sufficient amount of shRNA miR binds to its target, translation is significantly repressed. Thus, we expressed the shRNA miR from a separate plasmid which was always co-transfected with the original tuning construct. The reporter genes - i. e. Luc2 and hRluc - were also expressed from ...
Alteration of the mouse genome through homologous recombination in embryonic stem (ES) cells is the most accurate and versatile way to dissect gene function in a vertebrate model. Most often, a selectable marker is used to create a knockout allele by replacing an essential part of the gene. However, …
motivation= We aim at providing a clean, simple interface that will be self-intuitive and will allow researchers to submit high-quality scientific findings directly to a well established central repository (WormBase). Data will be publically shared and could be mined alongside data curated from the literature. This will ensure that every piece of federally funded research will be publicly available -including negative findings. We will pilot the submission focusing initially only on reporter gene fusions -the most common method to detect gene expression in c.elegans (WormBook chapter in preparation), and plan to expand the model to other gene expression localization experiments (e.g. ISH, smFISH, immunohistochemistry). If the model will prove to be successful, we will scale it up to include submission of other scientific findings (laser ablation experiments, rnai experiments..). guidelines for gene product localization experiments assessed with reporter gene fusions. The guidelines will be ...
We aim at providing a clean, simple interface that will be self-intuitive and will allow researchers to submit high-quality scientific findings directly to a well established central repository (WormBase). Data will be publically shared and could be mined alongside data curated from the literature. This will ensure that every piece of federally funded research will be publicly available -including negative findings. We will pilot the submission focusing initially only on reporter gene fusions -the most common method to detect gene expression in c.elegans (WormBook chapter in preparation), and plan to expand the model to other gene expression localization experiments (e.g. ISH, smFISH, immunohistochemistry). If the model will prove to be successful, we will scale it up to include submission of other scientific findings (laser ablation experiments, rnai experiments..). guidelines for gene product localization experiments assessed with reporter gene fusions. The guidelines will be modeled after the ...
Reporter genes are genes whose products can be readily assayed subsequent to transfection and used as markers for screening successfully transfected cells.
Tottenham Hotspur have a long-standing interest in Manchester United forward Anthony Martial, according to Peter Graves. The Red Devils forward has
Liam Payne has been accused of misunderstanding the true meaning behind LGBT Pride, after he featured in a new Adidas campaign. The former One Direction ...
The Fence Builders has recently opened its newest office and it promises to be the go-to place for all fencing needs. While being in the business for five years, the. ...
Today, the website of Mini PC News introduces the Best Gaming Mini PCs Under 300 Dollars 2017. The spokesman for this website says, The experts at budget Mini PCs are. ...
Easily study the dynamics of cellular processes with SBIs Cyto-Tracers™. Leverage SBIs high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors-available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The pCT-Mem-GFP (pCMV) Plasma Membrane Cyto-Tracer fuses a neuromodulin tag to copGFP, resulting in copGFP-labeling of the plasma membrane. The copGFP-fusion is expressed from the CMV promoter, for strong expression in common cell types such as HeLa, HEK293, and HT1080 ...
Easily study the dynamics of cellular processes with SBIs Cyto-Tracers™. Leverage SBIs high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors-available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The ER-Golgi Cyto-Tracer, pCT-Secretory-GFP (CMV) fuses a secretory consensus tag to copGFP, resulting in copGFP-labeling of the ER and Golgi. The copGFP-fusion is expressed from the CMV promoter, for strong expression in common cell types such as HeLa, HEK293, and HT1080 ...
Jill Abramson, executive editor of the New York Times, gave a frank overview of the evolving role of the newsroom as she described how the Times is blending traditional separations between print and web ...
Comment on attachment 573750 [details] [diff] [review] patch ,-// There are two kinds of TreeNode. Those that correspond to Reporters ,-// have more properties. The remainder are just scaffolding nodes for the ,-// tree, whose values are derived from their children. ,+// There are two kinds of TreeNode. ,+// - Leaf TreeNodes, that correspond to Reporters and have more properties. ,+// - Non-leaf TreeNodes are just scaffolding nodes for the tree; their values ,+// are derived from their children. Nit: Parallelism. Non-leaf TreeNodes, *which* are just scaffolding ,@@ -413,28 +420,26 @@ = function(a, b) { , * @return The built tree. , */ , function buildTree(aReporters, aTreeName) , { , // We want to process all reporters that begin with ,aTreeName,. First we , // build the tree but only fill the properties that we can with a top-down , // traversal. , ,- // Is there any reporter which matches aTreeName? If not, well create a ,- // dummy one. ,+ // Is there any reporter which ...
The LightSwitch 3´UTR Reporter Collection from Active Motif include over 12,000 3´UTRs available as transfection-ready luciferase reporter vectors for use in reporter assays.
Background Esophageal tumor (EC) is one of the deadliest cancers worldwide. protein levels. A dual-luciferase reporter gene assay was used to set up the interactions among circRNA-0008717, miR-203, and Slug. Results circRNA-0008717 expression was significantly upregulated in EC cells, and miR-2031 expression was decreased. Moreover, si-circRNA-0008717 or si-Slug inhibited the proliferation, migration, and invasion of EC cells. We found that circRNA-0008717 functioned as a sponge of miR-203, resulting in increased expression of Slug. We also reversed the effect of circRNA-0008717 knockdown on the EC progression by co-transfecting EC cells with a miR-203 inhibitor or Slug. Conclusions The proliferation, invasion, and migration of EC cells were enhanced by circRNA-0008717 sponging the miR-203 to increase Slug expression. was used to normalize the transcript levels of circRNA-0008717 and Slug. Relative expression is calculated using the 2-Ct method (24). Western blot analysis Total protein was ...
We were contacted recently with the following request which we are unable to fulfill:- ...................................................................... Hi, I am studying cis-regulation of a gene encoding nuclear protein. I am using beta-gal to show the expression pattern of this gene. But the result is not so good because beta-gal is expressed in cytoplasm, not nuclearly. Do you know any nuclear reporter gene I can use? Thank you very much! Zhe Han zhan at ...................................................................... If you are able to help please contact Zhe directly. Corniche.. the On-line life science resource. Free to use searchable database conatining 50,000 products from many sources large and small. ...
"Reporter genes have been used for several decades to study regulation of gene... *Hitra in zanesljiva dostava, plačilo tudi po povzetju.*
The behavior of single cells within a metabolic cycling population was visualized using phase-specific fluorescent reporters. The reporters largely recapitulated genome-specified mRNA expression profiles. A significant fraction of the cell population appeared to exhibit basal expression of the repor …
rhAmp SNP Master Mix includes unique Taq and RNase H2 enzymes. Reporter Mixes contain the universal reporter probe mix for signal generation in rhAmp SNP genotyping.
Dashboard: **selenium-daily-beta-CirrusSearch** job has junit reporter because **Test Result Trend** chart is visible. All other jobs dont have it. To enable it, **junit** reporter should be added to `tests/selenium/wdio.conf.js` file. Before: ```lang=js // Test reporter for stdout. // See also: reporters: [ spec ] ``` After: ```lang=js // Test reporter for stdout. // See: // // reporters: [ spec, [ junit, { outputDir: logPath, outputFileFormat: function () { const makeFilenameDate = new Date().toISOString().replace( /[:.]/g, - ); return `WDIO.xunit-${makeFilenameDate}.xml`; } } ] ], ``` , repository , job , reporter(s) , commit , status ,--,--,--,--,--, , mediawiki/core , [[ , selenium-daily-beta-MediaWiki ]] [[ ...
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
Deport the Middletons to Sandwich Islands: campaign gathers momentum : London A TV presenters quip about deporting problem families to the far flung archipelago has morphed into an exciting new project Sky News reporter Colin Braziers suggestion about sending 120000 miscreant families each costing the taxpaye
CBS Evening News reporter Steve Hartman took a deeper look into how his two young kids were computing the lyrics of country songs in their developing brains as
Project no. 911388 outlined 3 major aims to be addressed; Development of novel optical reporter systems, implementation of these reporters and probes in optical imaging of cancer and in translational therapeutic imaging. Results are very encouraging with publications resulting in prestigious journals. Gjerdrum et al. (2010) employed the use of novel imaging the impact of shRNA knockdown in vivo, while novel time-domain technique was described to image fluorescent reporters in vivo (Rosland et al. 2009, Torsvik et al. 2009). Combination of novel imaging strategy and near-infrared labeled mAbs and dextrans permitted visualization of drug uptake (McCormack et al. 2009), while Wang et al. (2010), Karlsen et. al. (2012) and 2 further manuscripts (McCormack et al. Submitted 2011/2) detail novel imaging strategies and reporters for in vivo imaging of patient xenografts. Translational application of cancer models and imaging are detailed in Erikstein et al. (2010), McCormack et al. (2010), and in ...
PrimCells is the reliable choice for your primary cell needs. We carry a variety of high quality primary cells, reporter cell lines and protein factors. Our uniquely design molecular tools provide you the most cost effective and reliable tool sets that can empower your research.
During 2019/20 the DPEA decided 90 per cent of appeals by initial written submissions and a site inspection within the 12-week target. The comparative figures were 82 per cent in 2018/19 and 72 per cent in 2017/18. The overall average time of 11 weeks for deciding appeals following this procedure compared with an average time of 11.6 weeks in 2018/19 and 12.6 weeks in 2017/18. In more complex appeals in which the reporter asked for further written submissions, the 20-week target was met in 73 per cent of cases with an average time of 20.7 weeks. This was a marked improvement over the two previous years.. A high proportion of complex cases, chiefly road orders, CPOs, section 36 cases and residential appeals contributed to the perennial problem of allocating cases to reporters and the scheduling of hearings.. The DPEA did not meet targets for cases conducted by hearings or inquiries, although the number of these cases was relatively small - 10 cases out of 369.. In respect of the eight local ...
EPA-induced translational up-regulation of reporter genes fused to BRCA1 mRNAb 5′UTR is dependent on the presence of tandem uORFsThe plasmid encoding for F-lu
GSK3β inhibitors are one of the few drugs identified in this screen that are capable of inhibiting EMT a Schematic of reporter system in MDA MB 231 reporter cells that were used to screen a panel of smallmolecule drugs In the assay cells that have a mesenchymallike phenotype express GFP green and those with epithelial cells express RFP red. Get Price ...
Available with Biological Response Genes AdenoExpress™ Available with Biological Response Genes Cytokines GM-CSF IFN-a IFN-g IL-2 IL-12
EXPRESS: If we want to use the regulatory aspects of NBB4 ethylene metabolism in a biosensor, we need to incorporate a reporter gene with strong, detectable output into our circuit. Generating such a reporter gene was the focus of EXPRESS, where we used error prone PCR on an existing blue iGEM chromoprotein amilCP to generate amilCP mutants with altered spectral characteristics ...
Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro ...
E2HSA exhibits GLP-1 receptor activating efficacy in NIT-1 cells. NIT-1 cells transiently transfected with Peak12 RIP-CRE 6x Luciferase reporter gene plasmid we
Community testing a must, she says State Rep. Liz Miranda is on the mend after testing positive for COVID-19 on May 1. The 39-year-old lawmaker, who has represented the 5th Suffolk District since Jan. 2019, talked to the Reporter this week about her experience with the virus, health disparities in Roxbury and Dorchester, and the importance of community testing. Of the 20 of
Amused at a couple of things in the Glen Rose Reporter article of June 2, 2016 that deals with Luminants not having paid the entire property tax ....
TY - JOUR. T1 - Coexpression of herpesviral thymidine kinase reporter gene and VEGF gene for noninvasive monitoring of therapeutic gene transfer. T2 - An in vitro evaluation. AU - Anton, Martina. AU - Wittermann, Constanze. AU - Haubner, Roland. AU - Simoes, Marcus. AU - Reder, Sybille. AU - Essien, Bryan. AU - Wagner, Bettina. AU - Henke, Julia. AU - Erhardt, Wolf. AU - Noll, Steffi. AU - Hackett, Neil R.. AU - Crystal, Ronald G.. AU - Schwaiger, Markus. AU - Gansbacher, Bernd. AU - Bengel, Frank M.. PY - 2004/10/1. Y1 - 2004/10/1. N2 - Coexpression of a reporter gene and a therapeutic gene may allow for noninvasive monitoring of cardiac gene therapy. We sought to evaluate the usefulness of an adenoviral vector expressing mutant herpesviral thymidine kinase reporter gene (HSV1-sr39tk) and vascular endothelial growth factor (VEGF) 121 in independent expression cassettes (Ad4tk). Methods: Accumulation of 14C-2′-fluoro-5-methyl-1-β-D-arabinofuranosyluracil (FIAU) and ...
OV-IA82Δ113 and OV-IA82Δ116 viruses were constructed by infecting OFTu cells (in T25 flasks) at a multiplicity of infection (MOI) of 1.0 with wild type OV-IA 82 for 3 hours and subsequently transfecting the cells with 10 μg of pSVP-113LF-EGFP-113RF and pSVP-116LF-EGFP-116RF transfer vectors by standard in vivo recombination protocols [28, 29]. Transfections were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions.. Viruses were harvested 48 h pi by scraping infected/transfected OFTu cells into sterile 15 ml conical tubes. The cell suspensions were vortexed, frozen/thawed 3 times, and then centrifuged at 1000 rpm, for 10 min at 4°C (Eppendorf Centrifuge 5810R, 15 amp version, Hamburg, Germany). The supernatant (viruses) were transferred into 2 ml cryogenic vials (Corning, NY, USA) and stored at -80°C for future use.. In order to select and purify recombinant viruses, limited dilution and plaque assays were performed. 3 ×104 of ...
The stimulation of reporter gene expression following co-transfection with the S4 gene of mammalian reoviruses was analysed. The σ3 protein of type 3 reovirus gave a five- to eightfold increase in expression of chloramphenicol acetyltransferase and β-galactosidase but this was found to be dependent upon the nature of the promoter being used to drive reporter gene expression. The σ3 protein of reovirus type 1 failed to stimulate reporter gene expression under any of the conditions used. Hybrid constructs between the S4 genes of reoviruses type 1 and 3 were used to map the stimulation characteristic to the carboxy-terminal third of the gene. Analysis of the level of σ3 protein accumulation in transfected cells showed that the reovirus type 3 protein accumulated to a much higher level than that of reovirus type 1. Using the hybrid gene constructs this higher level of protein accumulation was shown to co-segregate with the ability to stimulate reporter gene expression.
Reporter genes are a well-established method used to develop cell-based assays for testing drugs that inhibit targets involved in specific signaling pathways. Here, we introduce new PathHunter® Signaling Pathway Reporter Assays that utilize the industry-validated Enzyme Fragment Complementation (EFC) technology to detect reporter gene activity. Reporter cells with endogenous or heterologously introduced target receptors are used to readout signaling pathway activation, resulting in transcriptional activation of a reporter gene encoding a protein tagged with a small enzyme fragment. Reporter gene activity is measured by the addition of lysis buffer, luminescent enzyme substrate and the complementary larger enzyme fragment in an easy-to-use format. For example, an NF-κB reporter assay was developed and validated with CD40L to measure endogenous CD40 activation in U2OS cells. Another assay developed is the PathHunter Jurkat NFAT Pathway Reporter cell line, which measures T-cell activation but has ...
Want more hot BPR News stories? Sign up for our morning blast HERE. A news reporters live TV coverage was repeatedly interrupted by a man making offensive hand motions at the camera.. Fox News reporter Griff Jenkins was covering an anti-Trump protest in Albany, New York, on Monday when one protester approached and flipped the bird at the camera. ...
Signaling through the Notch1 receptor is essential for the control of numerous developmental processes during embryonic life as well as in adult tissue homeostasis and disease. Since the outcome of Notch1 signaling is highly context-dependent, and its precise physiological and pathological role in many organs is unclear, it is of great interest to localize and identify the cells that receive active Notch1 signals in vivo. Here, we report the generation and characterization of a BAC-transgenic mouse line, N1-Gal4VP16, that when crossed to a Gal4-responsive reporter mouse line allowed the identification of cells undergoing active Notch1 signaling in vivo. Analysis of embryonic and adult N1-Gal4VP16 mice demonstrated that the activation pattern of the transgene coincides with previously observed activation patterns of the endogenous Notch1 receptor. Thus, this novel reporter mouse line provides a unique tool to specifically investigate the spatial and temporal aspects of Notch1 signaling in vivo. ...
The assessment of how many cells have been successfully transfected or transduced in a cell population is a basic and critical evaluation parameter in many cell and molecular biology labs. Commonly, the cells of interest are transfected or transduced with a construct that results in the expression of a fluorescent protein (FP) reporter, such as GFP.
BioAssay record AID 95279 submitted by ChEMBL: Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by anti-CD28 and anti-TCR antibodies.
The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay allows you to use firefly luciferase, Nanoluc luciferase, or both, as primary reporters. This article provides information on when to use firefly and when to use Nanoluc luciferase as a primary reporter, and how both reporters can be used together to measure two biological responses in the same sample.
riyer at wrote: : I recd. so many requests from people who did not get my earlier post that I : decided to save time by re-posting it. At the outset I would like to state : that I am only a satisfied user of the above vectors and am not associated : with the company that markets them. And now here comes a readable version (with ,80 chars/line) :-) : Hi netters, I would like to report the availability of a set of : reporter gene vectors that have some distinct advantages over those : currently in use for the purpose of analysis of eukaryotic promoters : & enhancers. The vectors , SV40-Syncat, Syncat I, Syncat II, : SV40-pFlash, pFlash I and pFlash II, are as their names suggest : CAT & Luciferase (Luc) reporter gene vectors. What distinguishes : them from other vectors in this category is that these vectors produce : near-zero background. This feature is due to the fact, that they use a : modified SV40-t-intron as the donor for the poly-adenylation signal. : The wild type ...
A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple
The synthetic poly(A) signal in the pCAT3 vector and several other Promega vectors is placed upstream of the reporter to eliminate background and vector dependent reporter signal. Basically, all vectors have cryptic transcription factor binding sites that will cause the reporter to be transcribed. By putting in a polyadenylation signal, these transcripts should no longer contain the reporter gene because it is spliced off and will not cause artificial signal. I hope this is clear. If you have any additional questions, please feel free to contact me at [email protected] ...
Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gene reporter constr
Anew detection mechanism for the control of successful siRNA delivery to cells or tissue is introduced using a siRNA-based probe that is capable of inducing a ...
Thermo Scientific™ Gaussia-Dura Luciferase Reporter Assay Vectors pTK-Gaussia-Dura Luc Vector Thermo Scientific™ Gaussia-Dura Luciferase Reporter...
Data showing positive regulation of Wnt/β-catenin signal transduction by FAM129B siRNA using transcriptional reporter assay, target gene expression, and apoptosis assay
The EliKine™ Human IL-1β ELISA Kit is one of the latest scientific research products from Abbkine Scientific Co. Ltd. The company recently announced the launch of the product, designed to enhance scientific research processes.. The IL-1β is a member of the interleukin 1 cytokine family, produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). Interleukin 1β is an important mediator of the inflammatory response, involved in several cellular activities, including cell proliferation, differentiation, and apoptosis.. Interleukin 1β Elisa kit has human reactivity, employing a two-site sandwich ELISA to quantitate IL-1β in samples. The IL1B Elisa kit uses colorimetric method of detection, with the suitable samples types being Cell culture supernatants, other biological fluids, Plasma, and Serum.. The components of the kit include:. ...
Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine ...
The Global CM & CRM Devices Sales Market Report 2021 is a professional and in-depth study on the current state of the CM & CRM Devices Market. This report studies sales (consumption) of CM & CRM Devices in Global market, especially in North America, Europe, China, Japan, Southeast Asia and India, focuses on top players in these regions/countries, with sales, price, revenue and market share for each player in these regions, covering North America, Europe, China, Japan, Korea, Taiwan focuses on top manufacturers in global market, with production, price, revenue and market share for each manufacturer, covering Medtronic PLC , St. Jude Medical, Inc. , Boston Scientific Corporation , GE Healthcare , Philips Healthcare , Biotronik Se & Co., Kg , Cardiac Science Corporation , Livanova PLC , Mortara Instrument, Inc. and Schiller AG.. Request a Sample copy of this research @ Market Segment by Regions, this report splits Global into several key ...
Dc3 is a carrot lea-class gene belonging to a small gene family that encodes Dc3 and Dc3-like RNA sequences. We have examined the responsiveness to water deficit and abscisic acid (ABA) of the promoter/enhancer complex of Dc3 fused to a beta-glucuronidase (GUS) reporter gene in vegetative cells of transgenic tobacco. In 56-d tobacco, GUS expression in leaves increased about 200-fold during a 3-d d ...
Currently about 1,500,000 people die of AIDS and its complications each year. Many die of fungal infections - how many is not clear because of a lack of access to excellent diagnostic tests.. In Seattle, over 60 experts from major health agencies, including the World Health Organisation, UNAIDS, Bill and Melinda Gates Foundation, Clinton Health Access Initiative, Office for Global AIDS Coordination, Centers for Disease Control The Global Fund - and representatives from over 30 countries -- gathered to align on efforts to improve management of fungal infections. Delegates highlighted the lack of diagnostics and access to treatments, including the oldest antifungal drugs, as major hurdles to improvements in many developing countries.. A key output of the meeting based on a preliminary model created to analyze the costs associated with treatment and care of fungal infections in Africa, showed that early screening and treatment, before meningitis or pneumonia develop, is cost effective. If ...
PayPal is making one other acquisition within the e-commerce area because it strikes past funds and into bodily and on-line retail.. The digital funds large introduced a deal to accumulate start-up Pleased Returns on Thursday for an undisclosed quantity. The 120-person Santa Monica-based firm lets individuals return issues they purchased on-line, in particular person.. The post-purchase expertise is one thing we have been wanting into, because its such a ache level - individuals need to store on-line and return in retailer, and vice versa, Frank Keller, senior vp of client in-store and digital commerce at PayPal, informed CNBC in a telephone interview. For retailers, were offering extra complete providers past funds.. Thursdays deal follows PayPals $4 billion acquisition of Honey, a browser extension that lets customers discover and use coupons whereas purchasing on-line, in late 2019. PayPal additionally introduced a deal in March to purchase cryptocurrency safety firm, Curv, for $200 ...
Humorous views on interesting, bizarre and amusing articles, submitted by a community of millions of news junkies, with regular Photoshop contests.
Humorous views on interesting, bizarre and amusing articles, submitted by a community of millions of news junkies, with regular Photoshop contests.
Inverted repeats, or reverse complementary nucleotide sequences, can base pair with one another to form hairpin or cruciform structures that can stall replication fork progression and cause DNA damage. Inappropriate DNA repair events at such stalled replication sites could lead to fusion of inverted repeats and subsequently generate chromosomal rearrangements. To gain insight into the mechanisms underlying inverted repeat fusion, Hu and colleagues created reporter cassettes in which fusion of identical or mismatched inverted repeats would allow survival on selection media. Fusion of the identical and mismatched repeat reporters (IRR and MRR) occurred spontaneously in wild-type murine embryonic stem (ES) cells and led to unstable and complex chromosome rearrangements. DNA damaging agents increased fusion of the reporters, but γ-irradiation selectively induced IRR fusion and ultraviolet light selectively induced MRR fusion, suggesting that different pathways mediate inverted repeat fusion ...
Reporter gene assays are paramount for study of regulation of gene expression by gene regulatory elements (cis-acting factors), transcription factors or exogenous regulators (trans-acting factors).
Optical imaging of whole, living animals has proven to be a powerful tool in multiple areas of preclinical research and has allowed noninvasive monitoring of immune responses, tumor and pathogen growth, and treatment responses in longitudinal studies. However, fluorescence-based studies in animals are challenging because tissue absorbs and autofluoresces strongly in the visible light spectrum. These optical properties drive development and use of fluorescent labels that absorb and emit at longer wavelengths. Here, we present a far-red absorbing fluoromodule-based reporter/probe system and show that this system can be used for imaging in living mice. The probe we developed is a fluorogenic dye called SC1 that is dark in solution but highly fluorescent when bound to its cognate reporter, Mars1. The reporter/probe complex, or fluoromodule, produced peak emission near 730 nm. Mars1 was able to bind a variety of structurally similar probes that differ in color and membrane permeability. We ...
We show proof-of-principle examples that illustrate the user friendliness, speed, versatility, and genetic power of the MuLE system in primary mammalian cells. Complex genetic modulations involving combinatorial gene overexpression, knockdown, and knockout can be achieved using single viral infections. The simultaneous marking of infected cells with a variety of reporter cassettes facilitates cellular studies and in vivo imaging studies. By expressing multiple sgRNAs together with hCas9, single MuLE vectors are able to simultaneously target genetic mutations to multiple loci, providing a platform that harnesses the genetic power of the CRISPR/Cas9 system for performing combinatorial genetic manipulations. Combinatorial genetic screening experiments are also possible by combining libraries of Entry vectors with Entry vectors that encode defined genetic alterations. The ease of combining different genetic elements using this system represents a tool that will greatly facilitate systematic ...
Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins are encoded on a single transcript but are separated during translation by a 2A
High throughput (HTS) and high content screening (HCS) assays often utilize cell sources, which are not amenable to large-scale screening and can require extensive cell culture and processing prior to imaging. To overcome this hurdle, Hera and a commercial partner ArunA Biomedical, engineered human neural progenitors derived from stem cells with a non-viral vector encoding a GFP reporter gene using a selectable piggyBac™ gene editing system to produce hNP1 GFP+ cells. hNP1 reporter cells exhibit detectable dose dependent response to toxins tested, (cytotoxicity and cell migration) providing a sensitive, high throughput and high content amenable, cell based human neurotox assay platform.. ...
There was a positive correlation between CEA levels and CA 15-3 levels and patient prognosis. A high specificity for B-cells was confirmed by assaying for luciferase reporter gene expression from a plasmid containing an analogous combination of immunoglobulin heavy chain regulatory ...
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: 05-12-17 Brookhaven Reporter, Author: Reporter Newspapers, Name: 05-12-17 Brookhaven Reporter, Length: 24 pages, Page: 1, Published: 2017-05-11
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: JULY 2019 - Buckhead Reporter, Author: Reporter Newspapers, Name: JULY 2019 - Buckhead Reporter, Length: 32 pages, Page: 1, Published: 2019-06-19
An American news reporter gathering a story about the work of the Sisters of Charity in Calcutta asked one of the sisters where he might find Mother Teresa. The nun smiled for a moment, considering this Westerners question, then answered, You will find her with the poor. The reporter, trying to get to a more helpful, definitive response, then asked, Well, where might I find the poor? The nun answered, again with a bit of a smile, Why, they are everywhere. All about you. Cant you see them. Now frustrated, the reporter pressed on, Yes, yes, I know she works with the poor. But WHERE is Mother Teresa working with the poor? The nun - possibly with a bit of delight -, then answered, Wherever the poor might be found this morning! She paused for a moment then offered her hand to the reporter, Come, let us both go looking for some poor people ...
Microarray analysis and RNA-Seq are powerful tools for investigating differential gene expression in animal models of human genetic disease. Often, these models are loss of function mutations introduced by gene targeting or trapping. One difficulty in such experiments, especially where work on congenital malformations is concerned, is that whole embryo or regional dissections will commonly contain cells that do not express the gene of interest. Thus where cell autonomous effects are of primary interest, for example in the case of transcriptional regulators, such experiments will be compromised by dilutional effects and perhaps secondary effects on surrounding, non expressing tissues. Isolation of the relevant expressing lineage can mitigate these effects. Flow cytometry offers one route to cell purification but requires a fluorescent marker, and relatively few fluorescent protein gene reporters are available. FACS-Gal (1) offers the potential to capitalize upon the large existing repository of ...
BMG LABTECHs Microplate Readers have received Wavelength Based Dual Glow Reporter Genes certification for their outstanding performance. Read more!
HEK-Blue™ Null2 cells express the SEAP reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-kB and AP-1 binding sites. HEK-Blue™ Null2 is the parental cell line of several HEK-Blue™ TLR and HEK-Blue™ NOD cells.
To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA ... a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest ... In these cases, trans-acting elements, such as transcription factors are used to express the reporter gene. Reporter gene assay ... the reporter gene's expression is independent of the gene of interest's expression, which is an advantage when the gene of ...
Sherman started his 30 years on staff as a cub reporter covering nearly all the regular news beats from police and sheriff to ... He then worked as a rewrite man, a frontline general assignment reporter, leading feature story writer, war correspondent, in- ... depth investigative reporter and a foreign correspondent. He became a daily general interest writer of his page-2 column ... Cityside for seven years and a roving national and international assignment reporter. In 1964 he opened the London bureau as ...
"In National Event at Butler Tonight". Reporter-Times. February 19, 1940. p. 14. Retrieved August 22, 2019 - via ... "Gene Cramer". Peach Basket Society. October 30, 2015. Retrieved August 22, 2019. "Bill Cramer Statistics". Just Sports Stats. ... "Gene Cramer". Pro Basketball Encyclopedia. Retrieved August 22, 2019. "Bill Cramer NBL stats". Sports ... "Crawford Eugene Cramer dies at Brazil today". Reporter-Times. October 17, 1983. p. 12. Retrieved August 22, 2019 - via ...
Lolley, F. Dale (January 23, 2006). "Porter set tone early, put pressure on Plummer". Observer-Reporter. Archived from the ... Gene has an older brother, Tony, also an NFL official, who is a back judge currently assigned to Jerome Boger's officiating ... His father, Gene Steratore Sr., was a college football official and basketball referee. Steratore and his brother are the co- ... "Referee Gene Steratore Retiring From The NFL". WBZ-TV. June 22, 2018. Retrieved June 22, 2018. Borden, Sam (March 13, 2012). " ...
Staff Writer (5 September 2002). "Gene and the Gents are recognized 40 years after the showband era". Impartial Reporter. ... Gene Chetty (October 2002). "The Gene Chetty Story". The Irish Showbands Archive. Archived from the original on 21 August 2003 ... GMS Productions (5 September 2002). "Gene and the Gents Story (1964-70)". Retrieved 12 September 2006. Kennedy, F. "Gene and ... Gene and The Gents were a Northern Irish pop showband from Enniskillen, who achieved chart success with tracks including "The ...
McMillan, Graeme (October 5, 2020). "2020 Harvey Award Winners Revealed". Hollywood Reporter. Retrieved October 5, 2020. "SDCC ... Yang, Gene Luen; Pien, Lark; Yang, Gene Luen; Yang, Gene Luen (December 6, 2017). Boxers & saints. OCLC 825754024. Yang, Gene ... Official website Gene Yang at Library of Congress Authorities, with 9 catalog records Gene Yang at the Comic Book DB (archived ... Yang, Gene. "Gene Yang Speaks as Part of Graphic Novel Speakers Series". YouTube. Archived from the original on December 13, ...
"Gene Reynolds, Creative Architect Behind 'M*A*S*H' and 'Lou Grant,' Dies at 96". The Hollywood Reporter. Retrieved February 5, ... Gene Reynolds at IMDb Gene Reynolds at The Interviews: An Oral History of Television Who's Who at MGM - Gene Reynolds (1939) ... "Gene Reynolds - Awards & Nominations". Emmys. Retrieved February 5, 2020. Pedersen, Erik. "Gene Reynolds Dies: 'M*A*S*H' Co- ... "Gene Reynolds, Co-Creator of 'MASH,' Dies at 96". Variety. Retrieved February 5, 2020. "Gene Reynolds - Credits". TV Guide. ...
Rooney, David (23 January 2012). "Love Free or Die: Sundance Film Review". The Hollywood Reporter. Retrieved 17 July 2019. " ... Interview of Gene Robinson by Terry Gross of NPR Station WHYY's Fresh Air, January 2013 Op-ed piece in USA Today by Gene ... Gene Robinson (4 May 2014). "A Bishop's Decision to Divorce". The Daily Beast. "New Hampshire's Bishop Gene Robinson". NPR ( ... Gene Robinson" Online radio interview with Gene Robinson and Donald Armstrong, March 2007 Invocation at "We Are One" Concert ...
McNamee, Gregory (December 6, 2006). "Beyond the Epic.(Book review)". The Hollywood Reporter. The great crafter of epic films ... Gene D. Phillips, SJ". Jesuits - USA Midwest Province. 2016. "Gene D. Phillips, S.J." Loyola University of Chicago. Archived ... Carens, James F. (Spring 1976). "Gene D. Phillips, S. J." Evelyn Waugh Newsletter. X (1). Gene D. Phillips, S. J., gives us a ... Gene D. Phillips, S.J." Chicago Province of the Society of Jesus. Archived from the original on 2006-03-05. Kearney, George ( ...
"Gene LeBell, Famed Stuntman and "Godfather of Grappling," Dies at 89". The Hollywood Reporter. August 10, 2022. The Godfather ... Gene LeBell's Handbook of Self-Defense by Gene LeBell. 1996. Gene LeBell - The Grappling Club Master by Gene LeBell, Ben ... How to Break Into Pro Wrestling: "Judo" Gene LeBell's Insider Guide to the Biz by Gene Lebell and Mark Jacobs. 2003. Gene ... Gene LeBell at IMDb Gene LeBell biography Archived April 23, 2008, at the Wayback Machine - at the USA Traditional Kodokan Judo ...
"Packard Breaks World's Record". Independence Daily Reporter. August 10, 1908. Career statistics and player information from ... "Gene Packard". Society for American Baseball Research. Society for American Baseball Research. Retrieved 24 March 2016. " ... Baseball Reference, or Baseball Reference (Minors), or Retrosheet, or SABR Biography Project Gene Packard at Find a Grave v t e ...
"Gene Patton Dead: 'Gong Show' Dancing Machine Was 82". The Hollywood Reporter. Retrieved March 14, 2015. "Confessions of a ... April 25, 1932 - March 9, 2015), also known as Gene Patton and more widely known by his stage name Gene Gene the Dancing ... then announce the arrival of Gene Gene.[citation needed] The curtain would then rise. Patton would come out, moving his feet ... Gene Patton at IMDb (Articles with short description, Short description is different from Wikidata, Use mdy dates from May 2019 ...
"Gene Perret, Emmy-Winning Writer on 'The Carol Burnett Show,' Dies at 85". The Hollywood Reporter. November 23, 2022. "Gene ... Gene Perret Gene Perret (Articles with short description, Short description is different from Wikidata, 1937 births, 2022 ... Gene Perret (April 3, 1937 - November 15, 2022) was an American comedy writer and producer. He won three Emmys for his work on ...
Rose, Lacey (August 15, 2012). "A&E Cancels 'Gene Simmons Family Jewels'". The Hollywood Reporter. Retrieved August 16, 2012. " ... "Gene Simmons Family Jewels Episodes". TV Guide. Retrieved April 14, 2010. "Gene Simmons Family Jewels: Episode Guide". MSN TV. ... "Episode Detail: Gene's Handicap". Gene Simmons Family Jewels Episode Guide 2010 Season 5. TV Guide. Retrieved December 6, 2010 ... "A&E Re-ups for Season III of Gene Simmons Family Jewels". Gene Simmons. Archived from the original on January 24, 2013. ... Retrieved 20 October 2022. 10th Special Forces Group (AIRBORNE). "10th Special Forces Group - Airborne ... Gene Vance Jr. Foundation. "Gene Vance Jr. Foundation". Gene Vance Jr. Foundation. Retrieved 20 October 2022. "C. ... Gene's nephew, serves in the US Navy. During his life Gene actively sought to continue his family's military legacy. Gene A. ... "Gene Vance Jr. Foundation". Gene Vance Jr. Foundation. "C.A.R.E. Summit 2012". Naval Medical Center San Diego, CA. "Invitation ...
The Hollywood Reporter. Retrieved May 3, 2020. Yee, Lawrence (August 29, 2016). "Celebrities React to Gene Wilder's Death". ... Gene Wilder at Wikipedia's sister projects Media from Commons Data from Wikidata Gene Wilder at Curlie Gene Wilder at IMDb Gene ... Database Gene Wilder at the TCM Movie Database Gene Wilder at AllMovie The Gene Wilder Papers at the University of Iowa Gene ... Wilder, Gene. My French Whore. Thorndike Press, 2007. ISBN 0-7862-9725-5. Wilder, Gene. The Woman Who Wouldn't . St. Martin's ...
... reporter gene. The TEAD family in mammals includes four members, TEAD1, TEAD2, TEAD3, and TEAD4 that are transcription factors ... A fusion gene is an abnormal gene consisting of parts from two different genes that form as a result of a large scale gene ... consist of tumor cells that express a NUTM1 gene fused to one of the MADS-box gene family genes (i.e. a MXD4, MGA, or MXD1 gne ... The NUTM1 gene is located in band 14 on the long (or "q") arm of chromosome 15. In the early 1990's, this gene was implicated ...
"28 Sep 1994, 15 - The Reporter at". September 28, 1994. Retrieved January 28, 2022. "8 Oct 1996, Page 22 - The ... "Cubs Release Gene Clines". May 11, 1979. Retrieved January 28, 2022. "Gene Cline, World Series Winner With ... "Gene Clines, part of Pirates' 1971 World Series winner and MLB's first all-minority lineup, dies at 75". Pittsburgh Post- ... "Gene Clines, part of 1st MLB all-minority lineup, dies at 75". Associated Press. January 27, 2022. Retrieved January 27, 2022. ...
"Public Relations Veteran Gene Grabowski Joins kglobal". BusinessWire. Retrieved May 31, 2017. "Reporter Quits in Dispute Over ... "Gene Grabowski". IMDb. Retrieved August 1, 2017. "Roger Clemens takes advice from newest PR man - Gene Grabowski". Daily News. ... "Gene Grabowski". PR News Online. Retrieved August 21, 2017. "Hasbro ads to say it had no lead-paint recalls". NBC News. ... Gene Grabowski (born 1954) is an American former journalist, and a prominent expert on crisis communications within the public ...
"Genealogy Chat". Genes Reunited. Retrieved 11 March 2018. "Robert Williams, John Tucker, Theft > burglary, 13th November 1893 ... "The Conservative Ball". Croydon Advertiser and East Surrey Reporter - London. 23 February 1889. Retrieved 13 March 2018. "Fox ...
... making combinations of reporter and transgenes in the same locus problematic. The biggest disadvantage of using gene knock-in ... A gene knock-in therefore can be seen as a gain of function mutation and a gene knockout a loss of function mutation, but a ... Gene knock-in has allowed, for the first time, hypothesis-driven studies on gene modifications and resultant phenotypes. ... The speed of CRISPR/Cas9-mediated gene knock-in also allows for biallelic modifications to some genes to be generated and the ...
Kindred, Dave (2010). "Part II: "How Could Anyone Not Want to be a Reporter? Chapter 7. Gene Weingarten". Morning Miracle: ... Wikimedia Commons has media related to Gene Weingarten. Gene Weingarten on Twitter Gene Weingarten at The Washington Post ... Kindred, Dave (2010). "Part II: "How Could Anyone Not Want to be a Reporter? Chapter 7. Gene Weingarten". Morning Miracle: ... Weingarten, Gene (October 5, 2008). "Something About Harry: Gene Weingarten on Why Old Dogs Are the Best Dogs". The Washington ...
Exclusive)". The Hollywood Reporter. Archived from the original on May 7, 2021. Retrieved June 12, 2021. Kroll, Justin (April ... Maddaus, Gene (May 24, 2022). "Warner Bros. Considered Replacing Amber Heard in 'Aquaman 2'". Variety. Archived from the ... Aaron Couch of The Hollywood Reporter reported soon after that Michael Keaton had filmed a scene as his version of Bruce Wayne ... Borys Kit of The Hollywood Reporter reported then that there had not yet been serious discussions about a direct sequel to ...
G3: Genes,Genomes,Genetics. Established in 2011, G3 is an open access scientific journal which provides a forum for the ... The GSA Reporter, the Society's newsletter (published three times a year), keeps members apprised of Society activities. The ... "A message from the GSA President about the cancellation of TAGC". Genes to Genomes. 2020-03-16. Retrieved 2021-04-16. "We're ... The Society also publishes The GSA Reporter (formerly GENEtics), GSA's member newsletter. In 2013, the Society announced an ...
... performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene ... gene trap sequence tag, GTST) for the rapid identification of the disrupted gene. The International Gene Trap Consortium is ... Thus, gene traps simultaneously inactivate and report the expression of the trapped gene at the insertion site, and provide a ... the gene trap cassette is transcribed from the endogenous promoter of that gene in the form of a fusion transcript in which the ...
Robertson, Gene. "On the Beam" (film news and reviews). San Francisco Sun Reporter, Jul. 10, 1971. Dickinson, pp. 27-33 Del ...
... Lale, Max. S. "Cope, Millard Lewis". Handbook of Texas Online. Retrieved 2012-07-06. Roberts, Gene; Kunkel ... founder of the nearby Abilene Reporter. It later published as the Nolan County Review and became the daily Reporter in 1911 ... The Sweetwater Reporter is a newspaper based in Sweetwater, Texas, covering the Nolan County area of West Texas. Owned by ... In 2001, CNHI put the Reporter up for sale along with 30 other properties, including fellow West Texas papers the Big Spring ...
Maddaus, Gene; Lang, Brent (February 19, 2019). "Jussie Smollett Faces Prison, Career Ruin if He Lied About Attack". Variety. ... "Recording - Creed, Empire Top NAACP Image Award Nominations; Full List". The Hollywood Reporter. February 4, 2016. "Jussie ... Maddaus, Gene (April 4, 2019). "Chicago to Sue Jussie Smollett After He Refuses to Pay Investigation Costs". Variety. Retrieved ... "Why Isn't 'Empire's' Music Topping the Billboard Charts?". The Hollywood Reporter. February 25, 2015. Retrieved November 15, ...
Demby, Gene. "George Lucas: Hollywood Didn't Want To Fund 'Red Tails' Because Of Its Black Cast.", January ... Fernandez, Jay A. "Director picked for Lucasfilm project." The Hollywood Reporter, September 30, 2008. "George Lucas says ...
Gene Coughlan ... Editor, Dayton Daily News Bert Freed ... Colonel, U.S. Air Force. Stan Gordon ... Reporter. Marie Kenna ... ... who was in charge of answering UFO questions from reporters and the public. Intrigued by his experience, Greene decided to film ...
Gene Shue, American basketball player and coach (d. 2022) December 19 - Bud Clark, American politician and businessman (d. 2022 ... television news reporter (CBS Evening News) November 1 - Jack Morris, American football player (d. 2022) November 2 - Phil ...
In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to " ... Huang J, Ru B, Li S, Lin H, Guo FB (2010). "SAROTUP: scanner and reporter of target-unrelated peptides". J. Biomed. Biotechnol ... A versatile screening system for selective isolation of genes by specific gene-product/ligand interaction". Eur. J. Biochem. ... Parmley SF, Smith GP (1988). "Antibody-selectable filamentous fd phage vectors: affinity purification of target genes". Gene. ...
Maddeus, Gene (April 20, 2022). "Johnny Depp: Disney Wanted to Cut Ties on 'Pirates of the Caribbean' 'To Be Safe'". Variety. ... Brzeski, Patrick (May 28, 2017). "China Box Office: 'Pirates 5' Sails Off With $67.8M in Treasure". The Hollywood Reporter. ... Perez, Lexy (March 3, 2018). "Razzie Awards: 'Emoji Movie' Named Worst Picture of the Year". The Hollywood Reporter. Archived ... McClintock, Pamela (January 20, 2016). "Star Wars: Episode VIII Gets New Release Date". The Hollywood Reporter. Archived from ...
Maddaus, Gene (April 6, 2022). "Disney vs. Ron DeSantis: Why the Media Giant's Fight Over 'Don't Say Gay' Keeps Escalating". ... Kilday, Gregg (October 30, 2017). "Fox Animation Names Andrea Miloro, Robert Baird Co-Presidents". The Hollywood Reporter. ... Giardina, Carolyn (February 9, 2021). "Disney Shutting Blue Sky Animation Studio". The Hollywood Reporter. Archived from the ... Keegan, Rebecca (July 25, 2019). "Fox Animation Co-President Andrea Miloro Stepping Down". The Hollywood Reporter. Retrieved ...
Gene convinces Jeff and Buddy to help him with another scam in which Gene targets rich single men at bars and buys them drinks ... The Hollywood Reporter. Archived from the original on August 2, 2022. Retrieved August 2, 2022. Moreau, Jordan (August 1, 2022 ... One night, Gene targets Mr. Lingk, a man who has cancer. Despite feeling guilty, Gene continues with the usual plan. Buddy ... Francesca answers and updates Gene on events that have happened since he left Albuquerque. She tells Gene that the police ...
January 27 - Susan Aglukark, singer-songwriter January 29 - Sean Burke, ice hockey player February 26 - Gene Principe, sports ... reporter March 16 - Kevin Draxinger, swimmer April 5 - Gary Gait, lacrosse player April 5 - Paul Gait, lacrosse player and ...
Greg Gumbel, sportscaster for NFL on CBS Nia-Malika Henderson, CNN political reporter Lester Holt, journalist and anchor for ... Jazz Gene McDaniels: Jazz, pop Donnie McClurkin: Gospel Betty McGlown: R&B, pop Joe McPhee: Jazz Remy Ma: Hip hop Lee Michelle ... "18, Activist, Harvard-Bound: How Yara Shahidi Became the Face and Brain of Young Hollywood". The Hollywood Reporter. 8 August ... actor Gene Anthony Ray, actor Lance Reddick, actor Della Reese, actress, singer Rakim, rapper Daphne Maxwell Reid, actress and ...
"Wigan Reporter 'Music Scene' Article". Retrieved 3 May 2001. "Album Releases". "NME News Article". NME. Retrieved 28 July 2001 ... The Seahorses and Gene, before going on to self-release their debut single, "Quarantine", through Valiant Recordings on 23 ...
Show hosts: Dink Bernardi Larry Bledsoe Jack Bowen Robert D. "Bob" Coker Carole Arnold Jerry Bohnen Gene Collett Carlton ... formerly a reporter at CBS affiliate KWTV-TV) Mike "Road King" Rogers Scott Rowland Cynthia Rozmaryn David Rucker Mary Shea ... Jim Rupe Al Eshbach News reporters and anchors: Tim Allen Phil Bacharach Bill Bateman Natalie Bell Megan Bishop Jerry Bohnen ... Ken Johnson Craig Logsdon Beth Meyers Dan Mahoney Karen McCoy Gene Molter Bill Mondora Reid Mullins Mark Myers Derrick Nance ...
The Hollywood Reporter. Retrieved 24 June 2015. "Vogue Turkey November 2014". Pop Sugar. Archived from the original on 6 August ... Genes Reunited". "DNA Models Naomi Campbell". DNA Model Management LLC. Archived from the original on 13 November 2021. ...
Freese, Gene Scott (2014). Hollywood Stunt Performers, 1910s-1970s: A Biographical Dictionary, 2d ed. McFarland. p. 266. ISBN ... with Steele playing an aggressive reporter jumping on the running board of Kane's car. In the 1960s and 70s he remained active ...
"Emmys: Netflix Beats HBO With Most Nominations". The Hollywood Reporter. July 12, 2018. Retrieved July 12, 2018. "Twin Peaks". ... Mover Joe Adler as Roger Tammie Baird as Lorraine Jim Belushi as Bradley Mitchum John Billingsley as Doctor Ben Ronnie Gene ... Fienberg, Daniel (May 21, 2017). "'Twin Peaks': TV Review". The Hollywood Reporter. Retrieved May 22, 2017. Stephens, Emily L ... Abramovitch, Seth (April 20, 2020). "How I'm Living Now: David Lynch, Director". The Hollywood Reporter. Retrieved May 25, 2020 ...
There, he candidly discussed his thoughts about the political climate, unaware that reporters were also in the room and that ... Oberdorfer, p. 20 Oberdorfer, p. 174 Pearson, Drew (March 12, 1968), "Gene McCarthy Gets Praise for Views on McCarthyism", ... "Gene Vows He Won't Back HHH", St. Petersburg Times, St. Petersburg, Florida, p. 1, August 30, 1968 Macartney, Roy (August 31, ...
Foreword by Gene Roberts (First ed.). Denton, Texas: University of North Texas Press. ISBN 978-0929398846. LCCN 94043363. OCLC ... Scott Monserud, sports editor, Denver Post Mark Potok, reporter, spokesperson, Southern Poverty Law Center Steven Reddicliffe, ... was a Times Herald reporter at the time of John F. Kennedy assassination Margaret Mayer, who as chief of the Dallas Times- ... entertainment reporter Handbook of Texas Online, "Dallas Times Herald,". Retrieved January 7, 2009. "Courier-Journal has won". ...
Gene Nora Jessen (2002), "Ocean Flying", Powder Puff Derby Of 1929, Sourcebooks, p. 269, ISBN 9781402229725 Douglas 2015, p. ... reporter and pilot Lotfia Elnadi (1907-2002), first Egyptian woman to earn her pilot license 1933 Ruth Elder (1902-1977), pilot ...
But whenever the mild-mannered reporter would change into his true identity of Superman, Collyer's voice would deepen ... Gene Gropper Film Editor: Joseph Simon Sound Supervisor: Jim Bullock Music Composed and Conducted by John Gart Music Supervised ...
Video "Paul Taylor: Creative Domain: Film Review". The Hollywood Reporter. 12 September 2015. Retrieved 29 December 2015. ... Gene Moore, John Rawlings, William Ivey Long, Jennifer Tipton, Santo Loquasto, James F. Ingalls, Donald York and Matthew ...
Gene expression is imperfectly controlled, and it is possible that random fluctuations in the expression levels of many genes ... Vaidya A, Mao Z, Tian X, Spencer B, Seluanov A, Gorbunova V (July 2014). "Knock-in reporter mice demonstrate that DNA repair by ... A single gene may affect multiple traits. Some traits that increase fitness early in life may also have negative effects later ... Thus, harmful biological changes in old age may result from selection for pleiotropic genes that are beneficial early in life ...
Mace - Hotel Desk Clerk Pat Gleason as Banning - Bond Robber Gene O'Donnell as Bond Robber Donald Kerr as Reporter Howard M. ...
... a top-notch reporter played by Albert Brooks; and a charismatic but less seasoned reporter who later becomes a news anchor, ... After The Mary Tyler Moore Show ended, the show's creators, James L. Brooks and Allan Burns, along with Gene Reynolds, wrote a ... directed by William Wellman and written by former Chicago newspaper reporter Ben Hecht, is about New York newspaper reporter ... As a reporter working for Joseph Pulitzer in 1887, she went undercover by committing herself to Blackwell's Island Asylum, an ...
JAMIE ERDAHL NAMED LEAD REPORTER FOR THE SEC ON CBS "Brad Nessler has intriguing Jake Fromm theory, picks his No. 1". New York ... The duo are joined weekly by Jamie Erdahl, and former NFL referee Gene Steratore. He also provides play-by-play for college ... and sideline reporter Heather Cox. He also covered Saturday afternoon games for ESPN during the regular college basketball ... it was also announced that he would be teamed with former Penn State quarterback Todd Blackledge and sideline reporter Erin ...
Gene Porter (Stephen Collins) (season 2), one of the civic leaders of the town of Willoughby, the town doctor and the father of ... Stanhope, Kate (April 15, 2015). "'Revolution' to Be Resurrected Online". Hollywood Reporter. Retrieved April 15, 2015. Chapter ... Martin Shaw (Waleed Zuaiter), the member of the Patriots, who recruited Gene Porter to become the member of the Patriots. He ... The Hollywood Reporter. Retrieved June 30, 2012. "Freestyle/Hard Rock Amusement Park on NBC's Revolution". The Coaster Critic. ...
Hoover declined to use a spokesman, instead asking reporters to directly quote him and giving them handouts with his statements ... Herbert Hoover: A Life (2016), 464pp; comprehensive scholarly biography Smith, Gene. The Shattered Dream: Herbert Hoover and ... However, he changed his press policies after the 1929 stock market crash, screening reporters and greatly reducing his ...
The club never acknowledged his absence, simply declining to answer questions about his progress until reporters noticed ... as the replacement of guard Greg Horton with converted defensive lineman Gene Sanders and his greater speed was expected to ...
Caldwell and Gene S. Graham, Nashville Tennessean, "for their exclusive disclosure and six years of detailed reporting, under ... its fact-checking initiative during the 2008 presidential campaign that used probing reporters and the power of the World Wide ...
Gustines, George Gene (August 9, 2021). "Comic Book Writers and Artists Follow Other Creators to Substack". The New York Times ... As of late 2020, large numbers of journalists and reporters were coming to the platform, driven in part by the long-term ... a former PandoDaily tech reporter. Best and McKenzie describe Ben Thompson's Stratechery, a subscription-based tech and media ...
Some of the most common assays are: ELISAs Protein and cell growth assays Protein:protein interactions Reporter assays Nucleic ... based gene expression assays, as well as cell viability, cytotoxicity, and biorhythm assays based on the luminescent detection ... allows for detection of assays that contain multiple luminescent reporter enzymes, the development of new luminescence assays, ...
Reporter (uncredited) The Girl Next Door (1953) - Reporter (uncredited) The Kid from Left Field (1953) - Fan (uncredited) ... The Gene Krupa Story (1959) - Kenny Lemay (uncredited) Ma Barker's Killer Brood (1960) - Sheriff #1 Voyage to the Bottom of the ... Reporter (uncredited) My Lucky Star (1938) - Saier Five of a Kind (1938) - Reporter (uncredited) Submarine Patrol (1938) - ... Reporter (uncredited) The Grapes of Wrath (1940) - Joe Johnny Apollo (1940) - Reporter (uncredited) Star Dust (1940) - Desk ...
1969) La bohème (Giacomo Puccini): Fausto Cleva conducting the Metropolitan Opera Orchestra, with Richard Tucker and Gene ... but the Time reporter attributed the latter comment to Callas. According to John Ardoin, however, these two singers should ...
... news reporter) of WTEM Scott Linn (news reporter) of WTEM Marc "Nigel" Sterne (producer) Other notable guest co-hosts include: ... Felix Cavaliere, Eddie Brigati, Gene Cornish, and Dino Danelli: Kornheiser asserts that since he is able to recite the names of ... The sports director on WTEM, Andy Pollin, was both sidekick and news reporter of the show. Greg Garcia (who would later create ... Scott Linn replaced Sheehan as the news reporter. On June 2, WTEM announced that Kornheiser would do his last show on WTEM near ...
... reality television star and entrepreneur Gene Simmons will be the Keynote Q&A for the fifth annual Billboard Touring ... Gene Simmons to keynote tour conference. Kiss co-founder, reality television star and entrepreneur Gene Simmons will be the ... The Hollywood Reporter is a part of Penske Media Corporation. © 2022 The Hollywood Reporter, LLC. All Rights Reserved.. THE ... The Hollywood Reporter is a part of Penske Media Corporation. © 2022 The Hollywood Reporter, LLC. All Rights Reserved.. THE ...
The CEA N-A3 minigene has the potential to be used as a reporter gene for imaging cells in vivo. ... Purpose: Reporter genes can provide a way of noninvasively assessing gene activity in vivo. However, current reporter gene ... Reporter gene positive and negative Jurkat cells were used to establish xenografts in athymic mice. IHC showed staining of the ... Recombinant carcinoembryonic antigen as a reporter gene for molecular imaging Eur J Nucl Med Mol Imaging. 2009 Jan;36(1):104-14 ...
Reporter Gene Assay Market by Reagents & Assay kits (Luciferase, Green Fluorescent Protein, -glucuronidase, -galactosidase), ... Application (Gene Regulation, Protein Interaction, Cell Signalling Pathways), End Users, Region - Global Forecast to 2024 ...
Cardiac reporter gene imaging using the human sodium/iodide symporter gene. Cardiovasc Res. 2005;65:195-202. ... Three human genes (hNET, hNIS, and hdCKDM) and an established viral reporter gene (hsvTK), coupled with their corresponding ... Characterization of Reporter Gene-Transduced Primary Human T Cells. After transduction, reporter-bearing primary human T cells ... Conclusion: A comparison of different reporter gene-reporter probe systems for imaging of T cell number was performed, and the ...
... confirming the reporter assay as a reliable substitute for the standard antiviral assay. The Mx reporter gene assay also has ... Quantification of Atlantic salmon type-I interferon using an Mx1 promoter reporter gene assay. ... Quantification of Atlantic salmon type-I interferon using an Mx1 promoter reporter gene assay. ... Quantification of Atlantic salmon type-I interferon using an Mx1 promoter reporter gene assay ...
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Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. ... Animals, Cattle, Cell Line, Genes, Reporter, Green Fluorescent Proteins, Microscopy, Fluorescence, Plasmids, PPAR-beta, ... Gene reporter technology (GRT) has opened several new avenues for monitoring biological events including the activation of ... Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay.. Title. Plasmid ...
Health and Medicine Reporter Research American Journal of Human Genetics biovu Department of Medicine Human Genetics and ... Gene discoveries give new hope to people who stutter. Dec. 2, 2021, 10:34 AM. ... The research also turned up a stuttering-related gene implicated in autism-spectrum disorder, as well as genetic variants that ... Genomics Advances International Stuttering Project Jennifer (Piper) Below Reporter Dec 3 2021 Research Shelly Jo Kraft stutter ...
... in which the endogenous DGKθ promoter controls the expression of the luciferase reporter gene. To test the cell line, FXR, the ... in which the endogenous DGKθ promoter controls the expression of the luciferase reporter gene. To test the cell line, FXR, the ... in which the endogenous DGKθ promoter controls the expression of the luciferase reporter gene. To test the cell line, FXR, the ... in which the endogenous DGKθ promoter controls the expression of the luciferase reporter gene. To test the cell line, FXR, the ...
Ensuring both genetic reporters have different substrate requirements enables them to be detected using a single reaction ... Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability. ... Reporter-gene assays are used to study eukaryotic gene expression. ... Reporter-gene assays are used to study eukaryotic gene expression. Dual genetic reporters are commonly used in transient ...
Large-scale efforts are put into the generation of knockout mutant mice for many individual genes. Here, the authors ... Some mutant genes are expressed in the skin, whereas others are not, indicating systemic effects. One phenotype is affected by ... Skin-specific lacZ reporter gene expression. lacZ reporter gene expression in skin was determined in wholemount tissue ... A systematic review of skin ageing genes: gene pleiotropy and genes on the chromosomal band 16q24.3 may drive skin ageing. 30 ...
The first in a new class of gene-silencing drugs, known as inclisiran, has halved cholesterol levels in patients at risk of ... Reporter. Caroline Brogan Communications Division Contact details Tel: +44 (0)20 7594 3415 Email: [email protected] ... New gene silencer drug injections reduce cholesterol by 50% in clinical trial. by Caroline Brogan 17 March 2017 ... The first in a new class of gene-silencing drugs, known as inclisiran, has halved cholesterol levels in patients at risk of ...
DGAT gene depletion reduces SARS-CoV-2 protein synthesis without compromising viral genome replication/transcription. ... Mechanistically, viral nucleo capsid protein drives DGAT1/2 gene expression to facilitate LD formation and associates with ADRP ... Reporter gene assay. DGAT1 and DGAT2 promoter reporter clones were purchased from GeneCopoeia (Cat# HPRM47226-PG02 and ... b SARS-CoV-2-NP trans-activates DGAT1/2 genes. Huh7 cells were transfected with the indicated reporter-gene plasmid and viral ...
Learn about the reporter genes we are most excited about and how to apply them to your research! Including nuclear, optical, ... Our Reporter Genes. Having trouble deciding which reporter to use? Head over to our Choosing a Reporter page for our ... Our MISSION is to support research in regenerative medicine, gene therapy, oncolytic virotherapy and oncology drug discovery ...
... subunit gene fused to E. coli lacZ reporter gene. Together they form a unique fingerprint. ... subunit gene fused to E. coli lacZ reporter gene. ... Neuron-specific expression of reporter gene in transgenic mice ... Dive into the research topics of Neuron-specific expression of reporter gene in transgenic mice carrying the 5-upstream ...
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Dual Luciferase Reporter Assay. U2OS cells seeded in 24-well plates were co-transfected with 100 ng of reporter plasmid pGL4.20 ... Out of 2,542 genes, 898 genes were found to be upregulated in IRF8WT and the rest of the genes (1,644) were upregulated in IRF8 ... among various genes, the gene Ifnb1 (also known as Ifnb), which encodes interferon β (IFN-β), was found to be significantly ... genes in IRF8WT. (C) Volcano plot displaying the differential gene expression between the two groups of samples with ...
Video: Reporter loses a tooth on live TV - CNN Video. Most stock quote data provided by BATS. Market indices are shown in real ... Video: Reporter loses a tooth on live TV - CNN Video. Most stock quote data provided by BATS. Market indices are shown in real ... For more than 25 years, Silicon Valley Reporter has captivated our city with award-winning writing and groundbreaking reporting ...
We collect a broad range of reporter gene information on ... Browse latest articles and news on reporter gene. ... plants pai 1 gene reporter gene Indeed, reporter genes can also ... reporter gene. Browse latest articles and news on reporter gene. We collect a broad range of reporter gene information on ... reporter gene protein quantification reporter strips protein expression coli promoter gene expression ...
RT-PCR showed that EGCG increased the expression of natural moisturizing factor-related genes filaggrin (FLG), transglutaminase ... gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, ... Luciferase Reporter Gene Assay. HEK293 cells were seeded at 1.2 × 104 cells per well in 24-well plates. After 24 h, cells were ... We first confirmed the mRNA expression of NMF-related genes (FLG, TGM1, HAS-1, -2, and -3). EGCG increased the expression of ...
Health regardless of diet, lifestyle, and genes Keith Wommack - June 17, 2016. 0 ... Get Hawaii Reporter Email Updates Enter your email address to subscribe and receive notifications of new posts by email. ... In addition to his columns in Hawaii Reporter, he also contributes to CNN and Psychology Today. ...
The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection. ... They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest ...
A cell line that expresses a reporter gene in response to infection by Sindbis virus: A prototype for detection of positive ... that contains a defective Sindbis virus genome under the control of a Rous sarcoma virus promoter and the luciferase gene ... with Sindbis virus and provides a sensitive assay for titering variants of Sindbis virus that lack the structural protein genes ...
An Integrated Approach to Functional Genomics: Construction of a Novel Reporter Gene Fusion Library for Sinorhizobium meliloti ...
... a 2012 paper showing how the CRISPR system could be exploited to cut genes in a test tube and create a new method of gene ... Scientists debate the ethics of an unnerving gene-editing technique] Of course, a scientific agreement has no enforcing ... For starters, gene therapy has a troubled history. You make a mistake and the patient could die, which happened in the tragic ... If you knock out or modify a gene in a very young embryo, all the cells in that human being, including their reproductive cells ...
Researchers identified 20 genes with enhanced or muted expression in blood samples from dengue virus cases that progress to ... Dengue Severity Predicted by Gene Expression Signature in Patient Blood Jan 29, 2019 , staff reporter ... the set was whittled down to a set of 17 genes with lower-than-usual expression and three over-expressed genes in the severe ... As reported online today in Cell Reports, the team found that the 20-gene signature could also discern severe dengue cases in ...
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  • We here describe an assay for the detection of interferon-like activity in Atlantic salmon based on the transient transfection of chinook salmon embryo cells (CHSE-214 cells) with a rainbow trout Mx1 promoter linked to a luciferase reporter. (
  • Duplicate samples analysed using a cell protection assay for detection of antiviral activity correlated well with levels obtained by the Mx1 promoter reporter gene assay (R-2=0.97), confirming the reporter assay as a reliable substitute for the standard antiviral assay. (
  • The Mx reporter gene assay also has advantages in terms of sensitivity, high dynamic range and reliability over the conventional cell protection assay. (
  • Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. (
  • We aimed to investigate the mechanism by which EGCG promotes skin hydration by measuring hyaluronic acid synthase ( HAS ) and hyaluronidase ( HYAL ) gene expression and antioxidant and anti-pigmentation properties using cell proliferation assay, Western blotting analysis, luciferase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and reverse transcription polymerase chain reaction (RT-PCR) analysis. (
  • β-Galactosidase activity was determined by specific chemiluminescent reporter gene assay. (
  • RNA polymerase was shown to bind to the new promoter motif using a DNA-binding protein assay and proteomics enabled the discovery of four candidates to potentially function directly in control of transcription of the WLP and other key genes of C 1 fixation metabolism. (
  • QRT-PCR and luciferase reporter assay were used to verify the expression and target relationship of hsa_circ_0088196/LINC01492/miR-100-5p/LIF (leukemia inhibitory factor). (
  • Change transcription (RT) of 2 g RNA was eventually completed using 200 systems of SuperScriptIII invert transcriptase (Invitrogen, Carlsbad, CA). cDNA was amplified by BMS-509744 real-time PCR using IL-6 TaqMan Gene Appearance Assay (Identification# Hs00174131_m1). (
  • GAPDH Gene Appearance Assay (Identification# Hs99999905_m1) was utilized as an endogenous control. (
  • Luciferase reporter assay Cells had been transfected with pNF-B-luc (Stratagene, La Jolla, CA) and ether GFP (Clontech, Hill Watch, CA) (sunitinib tests) or pRL-TK (Promega, Madison, WI) (pazopanib tests) plasmids. (
  • Reporter-gene assays are used to study eukaryotic gene expression. (
  • Bioluminescent reporter systems using firefly and Renilla luciferases are widely used as co-reporters because both assays are easy and sensitive. (
  • Previously, luciferase reporter assays have been "flash" assays that must be read within seconds of reagent addition and require integrated injectors in the luminometer. (
  • With high-throughput, microfluidic-based quantitative RT-PCR assays, they profiled expression of the 20-gene signature in blood samples from these patients, successfully identifying severe dengue fever cases with or without additional warning signs. (
  • Learn how PathHunter® signaling reporter assays can be used to evaluate the cellular impact of therapeutics targeting a variety of proteins in NFkB, NFAT, and STAT3 signaling pathways. (
  • Utilize these easy-to-use cell-based, reporter gene assays to understanding the MOAs of therapeutics targeting TNFα, PD-1, IL-6, CD4, and RANK receptors. (
  • Applications include nucleic acid and protein quantifications, reporter-gene assays, cell counting, viability assays as well as confluence assessments. (
  • The NA3-Fc gamma RIIb recombinant gene, driven by a CMV promoter, was transfected in Jurkat (human T cell leukemia) cells. (
  • A beta-galactosidase gene under the control of a constitutively expressed beta-actin promoter was used as a transfection standard. (
  • To investigate the transcriptional regulation of the DGKθ gene, we used CRISPR/Cas9 to generate a DGKθ endogenous promoter luciferase reporter HepG2 cell line, in which the endogenous DGKθ promoter controls the expression of the luciferase reporter gene. (
  • One plasmid containing the experimental reporter gene (coupled to a regulated promoter) is cotransfected with a second plasmid containing a control reporter gene (coupled to a constitutive promoter). (
  • METHODS An E1/E3 deleted recombinant adenovirus (denoted AdCMVβGal) and an adenovirus with modified fibre structure (denoted AdZ.F(pk7)) both expressing the bacterial lacZ gene under the control of a human cytomegalovirus promoter were used for reporter gene expression in vitro and in vivo. (
  • CMV enh/prom: The human cytomegalovirus immediate-early gene 1 promoter/enhancer was originally isolated from the Towne strain and was found to be stronger than any other viral promoters. (
  • EM7 is a bacterial promoter that enables the constitutive expression of the antibiotic resistance gene in E. coli. (
  • Zeo: Resistance to Zeocin™ is conferred by the Sh ble gene from Streptoalloteichus hindustanus The Sh ble gene is driven by the CMV enhancer/promoter in tandem with the bacterial EM7 promoter allowing selection in both mammalian cells and E. coli . (
  • Here, we use differential RNA-sequencing to identify a previously undescribed promoter motif associated with essential genes for autotrophic growth of the model-acetogen Clostridium autoethanogenum . (
  • Next, in vivo experiments showed that a TetR-family transcriptional regulator (CAETHG_0459) and the housekeeping sigma factor (σ A ) activate expression of a reporter protein (GFP) in-frame with the new promoter motif from a fusion vector in E. coli . (
  • [ 17 ] Expression of an alkaline reporter gene injected on day E9.5 was easily detectable on day E12.5 using an EF1a promoter in a virus containing conserved late elements. (
  • Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. (
  • In recombinant DNA technology, these genes may be attached to a promoter region of interest. (
  • Scientists have turned a natural bacterial defense system into a laboratory tool for cutting or reordering genes in a cell - an innovation that could be used to target genetic mutations linked to numerous diseases. (
  • One area of competition will be in the interpretation of gene mutations. (
  • More than 260 mutations in the FOXL2 gene have been found to cause blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES). (
  • It is difficult to predict the type of BPES that will result from the many FOXL2 gene mutations. (
  • Five percent of mutations that cause BPES occur outside the FOXL2 gene in a neighboring region of DNA that normally controls the activity of the gene, known as a regulatory region. (
  • Approximately 12 percent of mutations causing BPES are deletions involving the FOXL2 gene. (
  • At least three mutations in the FOXL2 gene are thought to cause primary ovarian insufficiency without any other features of BPES. (
  • The FOXL2 gene mutations that cause primary ovarian insufficiency lead to a reduction in protein function, preventing the FOXL2 protein from controlling genes that regulate various activities in the ovaries. (
  • Some gene mutations are acquired during a person's lifetime and are present only in certain cells. (
  • AveXis, a Novartis company, today announced the US Food and Drug Administration (FDA) has approved Zolgensma® (onasemnogene abeparvovec-xioi) for the treatment of pediatric patients less than 2 years of age with spinal muscular atrophy (SMA) with bi-allelic mutations in the survival motor neuron 1 (SMN1) gene. (
  • This study aimed to determine the prevalence of occult HBV infection among Egyptian chronic HCV patients, the genotype and occurrence of surface gene mutations of HBV and the impact of co-infection on early response to treatment. (
  • Seven allelic mutations in the FIERY2 (FRY2) locus result in significant increases in the expression of stress-responsive genes with the DRE/CRT (drought-responsive/C-repeat) cis element but non-DRE/CRT type stress-responsive genes were less affected. (
  • mutations high frequency of IDH1/2 mutations in oligodendrogliomas, astrocytomas and in alteRations in the RB1 pathway in The TET2 gene encodes the -KG- secondary glioblastomas derived thereof low-gRade diffuse gliomas lacking dependent enzyme that catalyses suggests that these tumours share a common genetic alteRations the conversion of 5-methylcytosine to common progenitor cell population. (
  • Expression of p63 is almost exclusively restricted to epithelial cells, mutations in this gene are infrequent, and its expression is increased in a variety of solid tumors, particularly those of the head and neck area 12,13 . (
  • Hereditary vitamin D-resistant rickets is a very rare autosomal recessive disorder in which mutations of vitamin D receptor (VDR) coding genes cause failure or abnormal binding of vitamin D to VDRs. (
  • They know that Doudna helped invent an extraordinary new gene-editing technology, known as CRISPR. (
  • CRISPR is not the first method for manipulating genes, but it's by far the cheapest, easiest, most versatile. (
  • Their paper-which used CRISPR-editing tools in non-viable embryos that were destroyed after three days-is only the second published claim of gene editing in human embryos. (
  • Fan's team used CRISPR-Cas9 genome editing to introduce into some of the embryos a mutation that cripples an immune-cell gene called CCR5 . (
  • Two years later across the world in China, two girls, also as embryos, had their genes edited using CRISPR to make them (theoretically) resistant to HIV infection. (
  • The discovery that CRISPR-Cas9 could be harnessed and used to edit animal and human genes ushered in a new era of genetic research over the past several years. (
  • The CRISPR-Cas9 gene editing system has been known to produce extra cuts in wrong sections of DNA, which can interrupt cell function. (
  • Reporter genes can provide a way of noninvasively assessing gene activity in vivo. (
  • The CEA N-A3 minigene has the potential to be used as a reporter gene for imaging cells in vivo. (
  • The objective of the in vivo imaging studies was to determine the minimum number of T cells that could be visualized with the different nuclear reporter systems. (
  • The application of novel reporter systems (gene-probe combinations) together with noninvasive imaging modalities, such as PET, has shown the potential for monitoring T cells in vivo ( 1 - 4 ). (
  • To study the functions of large numbers of genes in vivo 2 requires a shift from gene-specific to genome-wide approaches. (
  • BACKGROUND/AIMS Replication deficient recombinant adenoviruses represent an efficient means of transferring genes in vivo into a wide variety of dividing and quiescent cells from many different organs. (
  • Therefore the ability to express normal and modified genes in the colon in vivo could provide a novel powerful tool for experimental studies and therapy of intestinal diseases such as inflammatory bowel disease. (
  • In an attempt to understand the molecular basis of carcinogenesis induced by these particles, we investigated the potential activation of activator protein-1 (AP-1) by crocidolite and freshly fractured or aged crystalline silica in a JB6 P + cell line stably transfected with AP-1-luciferase reporter plasmid (in vitro) and in AP-1-luciferase reporter transgenic mice (in vivo). (
  • Passage 10 (P10) MSC cells were transfected using plasmid vector containing GFP as reporter gene with different concentrations of DNA and lipofectamine. (
  • Sh fusion gene is provided in the expression plasmid pSelect-zeo which is selectable with Zeocin™ in mammalian and E.coli cells. (
  • DGAT gene depletion reduces SARS-CoV-2 protein synthesis without compromising viral genome replication/transcription. (
  • However, current reporter gene strategies may be limited by the immunogenicity of foreign reporter proteins, endogenous expression, or unwanted biological activity. (
  • Such gene delivery could be used to specifically express signal transduction proteins with therapeutic potential in inflamed colonic tissue. (
  • The glowing multicolour proteins that scientists currently use in fluorescence microscopy to track gene expression are not much help when it comes to observing what goes on in the deep tissues of the body. (
  • This transcription factor governs the expression of target genes that are involved in encoding cytokines, chemokines, growth factors, cell adhesion molecules, and acute-phase proteins that regulate inflammation, cell proliferation, and apoptosis. (
  • GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (
  • Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes. (
  • Differentially expressed RNAs were determined using the Limma package in R. Gene set enrichment analysis (GSEA) was performed using GSEA software (v. 3.0) and illustrated by ClusterProfiler and ggplot2 package in R. DAVID database (v. 6.8) was implemented to analyze functional categories and the association between genes and the corresponding Gene Ontology (GO) classification. (
  • The researchers brought together whole blood or peripheral blood mononuclear cell (PBMC) gene expression findings from seven prior studies, spanning nearly 450 dengue virus-infected individuals from five countries. (
  • Using an integrated multi-cohort analysis approach that "integrates biologically heterogeneous datasets to identify robust host gene signatures that are generalizable and prospectively validated," the researchers analyzed genome-wide expression data for 446 dengue virus patients from seven discovery datasets. (
  • After validating the 20-gene signature in three retrospective cohorts, the researchers followed 34 Colombians with dengue fever, including eight individuals who developed severe dengue and 17 patients with Zika co-infections or other "warning signs. (
  • More broadly, existing gene patents dissuaded researchers from studying sections of the genome that were already claimed, and high licensing fees discouraged would-be entrepreneurs. (
  • EU-backed researchers have used magnetic resonance imaging (MRI) to simultaneously track two different genes in two colours. (
  • Researchers in China have reported editing the genes of human embryos to try to make them resistant to HIV infection. (
  • Some researchers have proposed that the vanH , vanA , and vanX genes of hospital enterococci may have been acquired en bloc from the actinomycetes ( 8 ). (
  • Researchers at the University of Wisconsin-Madison have addressed many of those problems by packing a gene-editing payload into a tiny, customizable, synthetic nanocapsule. (
  • There is not any gene or set of genes related to all 11 issues, however the researchers discovered that various subsets have genetic similarities. (
  • Individuals with invasive breast cancer who had low scores on an investigational gene panel were just as likely to experience a recurrence if they received radiation therapy after breast-conserving surgery or not, Swedish researchers r. (
  • Based on the findings, researchers concluded that the schizophrenia risk conferred by the rare genetic variants found on the new genes they discovered and on those previously identified is conserved across ethnicities. (
  • When they examined genes that are necessary for the evolution of fins in zebrafish (a ray-finned fish that is a distant relative of coelacanth fishes) and compared them with the gene that regulates the development of limbs in mice, researchers found that zebrafish lacked the genetic mechanisms that are necessary for the development of fingers. (
  • Reporter gene positive and negative Jurkat cells were used to establish xenografts in athymic mice. (
  • Monitoring genetically altered T cells is an important component of adoptive T cell therapy in patients, and the ability to visualize their trafficking/targeting, proliferation/expansion, and retention/death using highly sensitive reporter systems that do not induce an immunologic response would provide useful information. (
  • Human T cells were transduced with retroviral vectors encoding for the human norepinephrine transporter (hNET), human sodium-iodide symporter (hNIS), a human deoxycytidine kinase double mutant (hdCKDM), and herpes simplex virus type 1 thymidine kinase (hsvTK) reporter genes. (
  • After viability and growth were assessed, 10 5 to 3 × 10 6 reporter T cells were injected subcutaneously on the shoulder area. (
  • The hNET/ meta- 18 F-fluorobenzylguanidine ( 18 F-MFBG) reporter system could detect less than 1 × 10 5 T cells because of its high uptake in the transduced T cells and low background activity. (
  • The hdCKDM/ 2′- 18 F-fluoro-5-ethyl-1-β- d -arabinofuranosyluracil ( 18 F-FEAU) and hsvTK/ 18 F-FEAU reporter systems detected approximately 3 × 10 5 T cells, respectively. (
  • A comparison of different reporter gene-reporter probe systems for imaging of T cell number was performed, and the hNET/ 18 F-MFBG PET reporter system was found to be the most sensitive and capable of detecting approximately 35-40 × 10 3 T cells at the site of T cell injection in the animal model. (
  • Genome-wide approaches to epidermal function include short interfering RNA-based genetic screens in cultured human epidermal cells 8 and RNA interference-mediated gene knockdown via in utero microinjection of lentiviral vectors 9 . (
  • Dual genetic reporters are commonly used in transient transfections of cultured cells to minimize experimental variability caused by differences in cell number, viability or transfection efficiency. (
  • The prospects of using recombinant adenoviruses for gene delivery into epithelial and subepithelial cells of the normal and inflamed colon are here analysed. (
  • In contrast, local administration of AdCMVβGal resulted in high reporter gene expression in colonic epithelial cells and lamina propria mononuclear cells. (
  • Furthermore, isolated lamina propria cells from mice with colitis infected in vitro showed a significant increase in reporter gene activity compared with controls. (
  • Finally, AdZ.F(pk7) adenoviruses with modified fibre structure produced 10- to 40-fold higher reporter gene activity in spleen T cells and lamina propria mononuclear cells of colitic mice compared with standard AdCMVβGal vectors. (
  • IMSEAR at SEARO: Generation of transgenic mesenchymal stem cells expressing green fluorescent protein as reporter gene using no viral vector in caprine. (
  • While there are many ways to label and track cells each with strengths and weakness, the green fluorescent protein (GFP) is a reporter gene commonly employed. (
  • Being able to map out real-time gene expression in our body's cells using contrasting colours would provide invaluable insight into currently invisible biological processes. (
  • A: The gene therapy in this trial, which was developed and first tested for safety by St. Jude Children's Research Hospital, manipulates a patient's stem cells by inserting a normal copy of the defective gene into the cell's genome. (
  • This gene replacement process is completed in a lab using a non-disease causing form of a virus - a viral vector - to carry the new genetic information into the cells. (
  • These cells will be transported to a cell manufacturing lab where DNA containing the normal gene will be transferred into the patient's cells. (
  • The number of T cells, B cells or myeloid cells marked by the vector used to insert the new gene will serve as early indicators of the therapy's success. (
  • The resulting abnormal gene activity accelerates the maturation of granulosa cells and causes the premature death of egg cells. (
  • The three GFP reporters mark an overlapping set of embryogenic cells, each with different fates i) differentiated embryos (DR5, LEC1 and GRP) and ii) unorganized callus (LEC1 and GRP) (Figure 1). (
  • Other studies using intraparenchymal cell transplantation under ultrasound image guidance [ 15 , 16 ] showed substantial expression by grafted cells of an alkaline phosphatase reporter gene. (
  • A new gene editing technique called prime editing, tested in human and mouse cells, rewrites DNA by only cutting a single strand to add, remove, or replace base pairs. (
  • Non-viral gene delivery systems capable of transfecting cells in the brain are critical in realizing the potential impact of nucleic acid therapeutics for diseases of the central nervous system. (
  • Address correspondence to Lisa rearrangements and rare variants that occur in the CYP2D6 gene. (
  • Chung said that Myriad's tests are well-regarded, but they've lagged in interpreting rare gene variants that each person has, but because they're so unique, have not been ascribed a clinical significance. (
  • A lot more analysis is had to pinpoint the precise results of the gene variants known within the find out about, however simply figuring out the genetics underlying their issues might supply convenience to a few sufferers with more than one psychological well being issues, Grotzinger mentioned. (
  • This dataset corresponds to a comprehensive splicing analysis of splice-site variants of exons 1 to 3 of the breast cancer susceptibility gene PALB2. (
  • Loss-of-function variants at the PALB2 gene are known to confer risk to breast and ovarian cancers. (
  • Sixteen variants were selected and genetically engineered into a PALB2 splicing reporter minigene. (
  • The study builds on a report published last year that identified 10 genes with rare variants that are directly tied to schizophrenia risk. (
  • The new genes, SRRM2 and AKAP11, contain rare protein-truncating variants (PTVs) that investigators say could be the cause of schizophrenia in some patients. (
  • Liu and his team used prime editing to target genes underlying Tay-Sachs disease and sickle cell anemia. (
  • The results indicated that these reagents could significantly regulate the expression of reporter luciferase. (
  • It was determined that only 179 bp 5′ of ospC was sufficient to regulate the reporter gfp in vitro in response to pH and temperature in B. burgdorferi B31. (
  • In Arabidopsis thaliana , two AUXIN RESPONSE FACTORs (ARFs), ARF7 and ARF19, positively regulate LR formation through activation of the plant-specific transcriptional regulators LATERAL ORGAN BOUNDARIES-DOMAIN 16/ASYMMETRIC LEAVES2-LIKE 18 ( LBD16/ASL18 ) and the other related LBD/ASL genes. (
  • These data indicate that FRY2 is an important negative regulator of stress gene transcription and suggest that structured RNA may regulate hormone and stress responses in plants as it does in animals. (
  • abstract = "Low temperature, drought, and high salinity induce the expression of many plant genes. (
  • CONCLUSIONS Local administration of recombinant adenoviruses with normal or modified fibre structure could provide a new reliable method for targeted gene expression in the inflamed colon. (
  • The technique, known as RNA interference (RNAi) therapy, essentially 'switches off' one of the genes responsible for elevated cholesterol. (
  • Our MISSION is to support research in regenerative medicine, gene therapy, oncolytic virotherapy and oncology drug discovery and to facilitate the widespread adoption and routine use of non-invasive imaging technologies in these endeavors. (
  • Doctors in New York Monday made the first attempt to treat Parkinson's disease with gene therapy. (
  • Although the gastrointestinal tract is a potentially attractive target for gene therapy approaches, only a few studies on the use of viral gene transfer vehicles in the gut have been reported. (
  • Our hope is that the gene therapy will produce a more robust immune system without the complications associated with transplant in these babies. (
  • Q: How does the gene therapy work? (
  • A: This is the first trial to combine gene therapy with low dose chemotherapy in patients with newly diagnosed XSCID, with hope of more complete and durable immune correction. (
  • Q: What are the anticipated benefits to using gene therapy over transplant in patients with XSCID? (
  • We are optimistic that gene therapy can achieve a more robust immune system too. (
  • Novartis dismissed the top two scientists at its gene therapy division shortly after CEO Vas Narasimhan learned of internal data falsification that has since snowballed into a damaging scandal, a person familiar with the situation said Wednesday. (
  • The person familiar with the situation, who spoke on condition of anonymity, confirmed that the departure of the Kaspars, who are brothers, was connected to the disclosure of data manipulation related to the gene therapy Zolgensma. (
  • The progression of gene therapy through clinical trials is contributing to millions of lives extended as well as more patients going into remission. (
  • The success gene therapy is playing towards these common cancers are making waves in the rare space as well. (
  • We have developed a reporter gene based on carcinoembryonic antigen (CEA), a human protein with limited normal tissue expression. (
  • Mechanistically, viral nucleo capsid protein drives DGAT1 / 2 gene expression to facilitate LD formation and associates with ADRP on the LD surface to complete the viral replication cycle. (
  • RGS3 potently inhibited G qα Q209L- and G 11α Q209 l -mediated activation of a cAMP-response element-binding protein reporter gene and G qα Q209L induced inositol phosphate production, suggesting that RGS3 efficiently blocks G qα from activating its downstream effector phospholipase C-β. (
  • The FOXL2 gene provides instructions for making a protein that attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (
  • Zolgensma is designed to address the genetic root cause of SMA by providing a functional copy of the human SMN gene to halt disease progression through sustained SMN protein expression with a single, one-time intravenous (IV) infusion. (
  • The light bulb represents a reporter gene, these are instructions for the cell to build a protein which is visible or measurable. (
  • Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (
  • Gene-testing companies will also compete to do the best job explaining often-ambiguous genetic results to their customers. (
  • Substantial genetic and biochemical similarities exist between resistance determinants in antimicrobial agent-producing actinomycetes and resistance genes found in gram-positive and gram-negative pathogens ( 6 - 9 ). (
  • To understand the mechanisms for the transcriptional activation of these genes, we conducted a reporter gene-aided genetic screen in Arabidopsis. (
  • Reverse genetic system, genetically stable reporter viruses and packaged subgenomic replicon based on a Brazilian Zika virus isolate. (
  • The vanH gene encodes a D‑lactate dehydrogenase that provides the requisite D‑lactate. (
  • The third gene, vanA , encodes an ATP-dependent D-Ala-D-Lac ligase. (
  • Increasing expression of a gene that encodes an enzyme responsible for a rate-limiting step. (
  • The Luc reporter is the expression of the firefly luciferase gene. (
  • InvivoGen has engineered a fusion between the firefly luciferase (Luc) and the Sh ble genes. (
  • LucSh: Synthetic LucSh fusion gene (LucSh-ΔCpG): InvivoGen has engineered a fusion between the firefly luciferase gene and the Sh ble gene conferring Zeocin™ resistance. (
  • But the typical method for delivering gene therapies to specific tissues in the body can be complicated and may cause troubling side effects. (
  • The Alliance for Regenerative Medicine's (ARM) Gene Editing Task Force on Tuesday released a set of principles for human genome editing endorsed by thirteen of its members who are involved in the development of gene therapies or gene-editing technology. (
  • Owing to technical limitations, a large-scale high-throughput systematic functional screen for genes involved in skin homeostasis was not feasible until recently. (
  • Understanding gene regulation with high-throughput. (
  • Furthermore, nucleotide sequences related to the cluster vanHAX are present in this DNA, suggesting that the prolonged use of avoparcin in agriculture led to the uptake of glycopeptide resistance genes by animal commensal bacteria, which were subsequently transferred to humans. (
  • A cluster and humans coming into contact with the animals (farm that includes three genes, vanH , vanA , and vanX , is required for high-level resistance to glycopeptides. (
  • There's all sorts of ways to make money,' said Dan Ravicher, executive director of the Public Patent Foundation and a plaintiff in the lawsuit, which pitted civil rights activists and patient groups against Myriad Genetics, a Utah provider of tests on its patented breast-cancer-risk genes. (
  • Type I IFN production subsequently leads to induction of ISGs (interferon stimulated genes), which in turn initiate a series of downstream signaling cascades essential for mounting efficient antiviral immune response ( 14 ). (
  • During tumor-associated angiogenesis, the balance of angiogenesis stimulators and inhibitors is tipped in favor of angiogenesis by hypoxia-inducible factor-1 gene expression [ 2 ]. (
  • Borrelia burgdorferi regulates genes in response to a number of environmental signals such as temperature and pH. (
  • In April 2015, a different China-based team announced that they had modified a gene linked to a blood disease in human embryos (which were also not viable, and so could not have resulted in a live birth). (
  • The firefly Luc gene is a highly sensitive reporter gene and thus is ideal for detecting low-level gene expression. (
  • Herpes simplex virus type 1 thymidine kinase (hsvTK) and mutant hsv1sr39TK reporter genes have been used in most of these seminal studies. (
  • Some mutant genes are expressed in the skin, whereas others are not, indicating systemic effects. (
  • Curated mutant alleles for the specified gene, listed alphabetically. (
  • Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (
  • We determined the imaging sensitivity (lower limit of T cell detection) of each reporter using appropriate radiolabeled probes for PET or SPECT imaging. (
  • To test the cell line, FXR, the transcription factor for upregulating the expression of DGKθ gene, was used to validate the cell line. (
  • Furthermore, the reported agonists for the expression of DGKθ, cAMP and GW4064, the known inhibitor for DGKθ enzyme activity, R59949, and a potential regulator for DGKθ enzyme expression, EGCG (the major catechin in green tea), were applied to the reporter cell line. (
  • Or they carry the gene for Huntington's disease or some other dreadful time bomb wired through every cell in their body. (
  • As reported online today in Cell Reports , the team found that the 20-gene signature could also discern severe dengue cases in retrospective studies involving 84 infected individuals from three countries and in a prospective analysis of longitudinal samples from nearly three dozen dengue patients from Colombia. (
  • Gene expression lets us know what each cell is doing," states the study's lead author Dr Hyla Allouche-Arnon, also from the Weizmann Institute of Science. (
  • A specific somatic mutation in the FOXL2 gene has been found in a type of ovarian cancer that occurs in adulthood called adult granulosa cell tumor. (
  • Main objective in this project has been to isolate and characterize the different embryogenic cell types in Brassica napus microspore culture using a set of fluorescently-tagged gene reporters. (
  • 1. Define and collect the different embryogenic cell types present in microspore culture using GFP-based reporters, time-lapse imaging, and cell sorting. (
  • Time-lapse imaging of the embryo-expressed GFP reporter lines was used to determine the fate of the different embryogenic cell types in culture. (
  • While the principles endorse somatic cell gene editing and the development of regulatory standards for gene editing, the document asserts that it is too early to support any form of human germline gene editing due to unanswered ethical, legal and safety questions. (
  • InvivoGen provides a wide range of high-quality reagents for tissue and cell culture applications , including mycoplasma detection , prevention and elimination, plus various antimicrobial agents , selective antibiotics , reporter detection kits , the transfection reagent LyoVec™ , the transfection enhancer NATE™ , and an endotoxin detection kit . (
  • Gene reporter technology (GRT) has opened several new avenues for monitoring biological events including the activation of transcription factors, which are central to the study of nutrigenomics. (
  • The lack of DNA cleavage results in gene silencing through impeding RNA polymerase binding, transcription factor binding, and/or transcription elongation. (
  • The specific regulation of DRE/CRT class of genes by FRY2 appears to be caused by repression of stress induction of the upstream CBF/DREB transcription factor genes. (
  • The actual origins of acquired resistance genes are unknown, but environmental microbes, including the strains producing antimicrobial agents, are believed to be important sources ( 4 , 5 ). (
  • It already has been used to develop a mushroom that won't turn brown as quickly, which the U.S. Department of Agriculture has decided doesn't need regulatory approval because no genes from other organisms were required for its creation. (
  • Incorporating genes from other organisms into the host. (
  • 100 mutants) for phenotypes in a specific tissue via targeted gene knockouts has not been reported previously. (
  • The team genetically engineered two groups of highly active reporter genes, each group expressing one of two specially designed enzymes. (
  • European regulators have narrowed the indication for both Vectibix and Erbitux to include treating only those patients whose tumor carries the wild-type KRAS gene. (
  • The roles of the 20 gene products in the pathogenesis of severe dengue will be studied in the future," the authors wrote, noting that many of them are implicated in various aspects of antiviral innate immune responses. (
  • Sh fusion gene exhibits a higher luciferase activity and enables a better and faster selection of Zeocin™ resistant clones. (
  • To construct a CEA reporter gene for PET, a CEA minigene (N-A3) was fused to the extracellular and transmembrane domains of the human Fc gamma RIIb receptor. (
  • Therefore, we focused on human reporter gene systems that have the potential for translation to clinical studies. (
  • Beyond the absurdity of gene patents - imagine patenting gold, the human arm, or gravity - they said that patents had hurt patients, stifled business and stunted research. (
  • RESULTS Intravenous or intraperitoneal injection of AdCMVβGal into healthy Balb/c mice caused strong reporter gene expression in the liver and spleen but not in the colon. (
  • Diazinon immunotoxicity in mice: Modulation of cytokines level and their gene expression. (
  • The IMPC applies a panel of phenotyping screens to characterise single-gene knockout mice by comparison to wild types. (
  • Resistance arises by mutation (influencing the target or efflux of the antimicrobial agent) or by the acquisition of resistance genes (encoding antimicrobial or target alteration, or alternate pathways) ( 2 , 3 ). (
  • The R visualization package GOPlot was used to get a better visualization of the relationships between genes and the selected functional categories. (
  • Hudson RC, Gray C, Kiss-Toth E, Chico TJA & Qwarnstrom EE (2012) Bioinformatics Analysis of the FREM1 Gene-Evolutionary Development of the IL-1R1 Co-Receptor, TILRR. (
  • While MRI today enables scientists to observe such deep-seated processes, the grayscale images produced do not provide specific information about gene expression. (
  • Another type of gene editing that doesn't rely on DNA breaks and was thought to minimize inaccuracy is base editing , in which an enzyme can trade one DNA nuclease for another, but this strategy offers limited options as it can only make four of the 12 possible base pair changes, and some recent work has suggested it's not as precise as scientists first thought. (
  • Scientists have identified two new genes linked to schizophrenia and discovered that a third gene previously known to be involved in the disorder may also play a role in autism . (
  • This suggests that regulation of gene expression in response to pH and temperature may vary between these two B. burgdorferi strains. (
  • The third gene flagged in the study, PCLO, was previously implicated in schizophrenia but is now identified as having a shared risk for schizophrenia and autism. (
  • They are one of the most efficient methods of a gene delivery vector. (
  • Nonetheless, he's excited by the new tool and "enamored" of some aspects of the new technology, such as its potential ability to edit a greater diversity of targets than other gene editing methods can. (