Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Proteases which use a metal, normally ZINC, in the catalytic mechanism. This group of enzymes is inactivated by metal CHELATORS.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Strongly cationic polymer that binds to certain proteins; used as a marker in immunology, to precipitate and purify enzymes and lipids. Synonyms: aziridine polymer; Epamine; Epomine; ethylenimine polymer; Montrek; PEI; Polymin(e).
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Antibodies reactive with HIV ANTIGENS.
Endoscopic examination, therapy or surgery of the female pelvic viscera by means of an endoscope introduced into the pelvic cavity through the posterior vaginal fornix.
Persons or entities that introduce a novel composition, device, or process, as well as improvements thereof.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
Plants or plant parts which are harmful to man or other animals.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A malignant neoplasm that may be classified either as a glioma or as a primitive neuroectodermal tumor of childhood (see NEUROECTODERMAL TUMOR, PRIMITIVE). The tumor occurs most frequently in the first decade of life with the most typical location being the cerebellar vermis. Histologic features include a high degree of cellularity, frequent mitotic figures, and a tendency for the cells to organize into sheets or form rosettes. Medulloblastoma have a high propensity to spread throughout the craniospinal intradural axis. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2060-1)
Time period from 1901 through 2000 of the common era.
Primary or metastatic neoplasms of the CEREBELLUM. Tumors in this location frequently present with ATAXIA or signs of INTRACRANIAL HYPERTENSION due to obstruction of the fourth ventricle. Common primary cerebellar tumors include fibrillary ASTROCYTOMA and cerebellar HEMANGIOBLASTOMA. The cerebellum is a relatively common site for tumor metastases from the lung, breast, and other distant organs. (From Okazaki & Scheithauer, Atlas of Neuropathology, 1988, p86 and p141)
Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
7-Hydroxycoumarins. Substances present in many plants, especially umbelliferae. Umbelliferones are used in sunscreen preparations and may be mutagenic. Their derivatives are used in liver therapy, as reagents, plant growth factors, sunscreens, insecticides, parasiticides, choleretics, spasmolytics, etc.
Individual's rights to obtain and use information collected or generated by others.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Iron-containing proteins that are widely distributed in animals, plants, and microorganisms. Their major function is to store IRON in a nontoxic bioavailable form. Each ferritin molecule consists of ferric iron in a hollow protein shell (APOFERRITINS) made of 24 subunits of various sequences depending on the species and tissue types.

Transcriptional repression by the Drosophila giant protein: cis element positioning provides an alternative means of interpreting an effector gradient. (1/16045)

Early developmental patterning of the Drosophila embryo is driven by the activities of a diverse set of maternally and zygotically derived transcription factors, including repressors encoded by gap genes such as Kruppel, knirps, giant and the mesoderm-specific snail. The mechanism of repression by gap transcription factors is not well understood at a molecular level. Initial characterization of these transcription factors suggests that they act as short-range repressors, interfering with the activity of enhancer or promoter elements 50 to 100 bp away. To better understand the molecular mechanism of short-range repression, we have investigated the properties of the Giant gap protein. We tested the ability of endogenous Giant to repress when bound close to the transcriptional initiation site and found that Giant effectively represses a heterologous promoter when binding sites are located at -55 bp with respect to the start of transcription. Consistent with its role as a short-range repressor, as the binding sites are moved to more distal locations, repression is diminished. Rather than exhibiting a sharp 'step-function' drop-off in activity, however, repression is progressively restricted to areas of highest Giant concentration. Less than a two-fold difference in Giant protein concentration is sufficient to determine a change in transcriptional status of a target gene. This effect demonstrates that Giant protein gradients can be differentially interpreted by target promoters, depending on the exact location of the Giant binding sites within the gene. Thus, in addition to binding site affinity and number, cis element positioning within a promoter can affect the response of a gene to a repressor gradient. We also demonstrate that a chimeric Gal4-Giant protein lacking the basic/zipper domain can specifically repress reporter genes, suggesting that the Giant effector domain is an autonomous repression domain.  (+info)

Accelerated accumulation of somatic mutations in mice deficient in the nucleotide excision repair gene XPA. (2/16045)

Inheritable mutations in nucleotide excision repair (NER) genes cause cancer-prone human disorders, such as xeroderma pigmentosum, which are also characterized by symptoms of accelerated ageing. To study the impact of NER deficiency on mutation accumulation in vivo, mutant frequencies have been determined in liver and brain of 2-16 month old NER deficient XPA-/-, lacZ hybrid mice. While mutant frequencies in liver of 2-month old XPA-/-, lacZ mice were comparable to XPA+/-, lacZ and the lacZ parental strain animals, by 4 months of age mutant frequencies in the XPA-deficient mice were significantly increased by a factor of two and increased further until the age of 16 months. In brain, mutant frequencies were not found to increase with age. These results show that a deficiency in the NER gene XPA causes an accelerated accumulation of somatic mutations in liver but not in brain. This is in keeping with a higher incidence of spontaneous liver tumors reported earlier for XPA-/- mice after about 15 months of age.  (+info)

Inducible genetic suppression of neuronal excitability. (3/16045)

Graded, reversible suppression of neuronal excitability represents a logical goal of therapy for epilepsy and intractable pain. To achieve such suppression, we have developed the means to transfer "electrical silencing" genes into neurons with sensitive control of transgene expression. An ecdysone-inducible promoter drives the expression of inwardly rectifying potassium channels in polycistronic adenoviral vectors. Infection of superior cervical ganglion neurons did not affect normal electrical activity but suppressed excitability after the induction of gene expression. These experiments demonstrate the feasibility of controlled ion channel expression after somatic gene transfer into neurons and serve as the prototype for a novel generalizable approach to modulate excitability.  (+info)

An Arabidopsis 14-3-3 protein can act as a transcriptional activator in yeast. (4/16045)

The 14-3-3 proteins are a group of highly conserved and widely distributed eukaryotic proteins with diverse functions. One 14-3-3 protein, AFT1 from Arabidopsis thaliana, was found to be able to activate transcription in yeast. When fused to the DNA-binding domain of a bacterial protein LexA, AFT1 can activate transcription of reporter genes that contain LexA operator sequences in their promoters. Although the in vivo function of AFT1 is not completely known, its similarity to previously identified proteins found in transcription complexes of Arabidopsis and maize suggests that AFT1 and some other 14-3-3 proteins may activate gene expression in other systems as well.  (+info)

The dually acylated NH2-terminal domain of gi1alpha is sufficient to target a green fluorescent protein reporter to caveolin-enriched plasma membrane domains. Palmitoylation of caveolin-1 is required for the recognition of dually acylated g-protein alpha subunits in vivo. (5/16045)

Here we investigate the molecular mechanisms that govern the targeting of G-protein alpha subunits to the plasma membrane. For this purpose, we used Gi1alpha as a model dually acylated G-protein. We fused full-length Gi1alpha or its extreme NH2-terminal domain (residues 1-32 or 1-122) to green fluorescent protein (GFP) and analyzed the subcellular localization of these fusion proteins. We show that the first 32 amino acids of Gi1alpha are sufficient to target GFP to caveolin-enriched domains of the plasma membrane in vivo, as demonstrated by co-fractionation and co-immunoprecipitation with caveolin-1. Interestingly, when dual acylation of this 32-amino acid domain was blocked by specific point mutations (G2A or C3S), the resulting GFP fusion proteins were localized to the cytoplasm and excluded from caveolin-rich regions. The myristoylated but nonpalmitoylated (C3S) chimera only partially partitioned into caveolin-containing fractions. However, both nonacylated GFP fusions (G2A and C3S) no longer co-immunoprecipitated with caveolin-1. Taken together, these results indicate that lipid modification of the NH2-terminal of Gi1alpha is essential for targeting to its correct destination and interaction with caveolin-1. Also, a caveolin-1 mutant lacking all three palmitoylation sites (C133S, C143S, and C156S) was unable to co-immunoprecipitate these dually acylated GFP-G-protein fusions. Thus, dual acylation of the NH2-terminal domain of Gi1alpha and palmitoylation of caveolin-1 are both required to stabilize and perhaps regulate this reciprocal interaction at the plasma membrane in vivo. Our results provide the first demonstration of a functional role for caveolin-1 palmitoylation in its interaction with signaling molecules.  (+info)

Fibroblast growth factor-8 expression is regulated by intronic engrailed and Pbx1-binding sites. (6/16045)

Fibroblast growth factor-8 (FGF8) plays a critical role in vertebrate development and is expressed normally in temporally and spatially restricted regions of the vertebrate embryo. We now report on the identification of regions of Fgf8 important for its transcriptional regulation in murine ES cell-derived embryoid bodies. Stable transfection of ES cells, using a human growth hormone reporter gene, was employed to identify regions of the Fgf8 gene with promoter/enhancer activity. A 2-kilobase 5' region of Fgf8 was shown to contain promoter activity. A 0.8-kilobase fragment derived from the large intron of Fgf8 was found to enhance human growth hormone expressed from the Fgf8 promoter 3-4-fold in an orientation dependent manner. The intronic fragment contains DNA-binding sites for the AP2, Pbx1, and Engrailed transcription factors. Gel shift and Western blot experiments documented the presence of these transcription factors in nuclear extracts from ES cell embryoid bodies. In vitro mutagenesis of the Engrailed or Pbx1 site demonstrated that these sites modulate the activity of the intronic fragment. In addition, in vitro mutagenesis of both Engrailed and Pbx1 sites indicated that other unidentified sites are responsible for the transcriptional enhancement observed with the intronic fragment.  (+info)

Regulation of human hsp90alpha gene expression. (7/16045)

Mammalian HSP90alpha and HSP90beta are encoded by two individual genes. On the basis of the upstream sequences of the human hsp90alpha gene, GenBank accession number U25822, we have constructed CAT reporter plasmids driven by individual fragments of the hsp90alpha gene. We found that (1) the proximal heat shock element complex located at -96/-60 enhances hsp90alpha promoter expression; (2) heat shock induction depends upon the coexistence of distal heat shock element at -1031/-1022 and the proximal heat shock element complex of the hsp90alpha gene; (3) unlike hsp90beta, downstream sequences of the transcription start site inhibit hsp90alpha expression. We conclude that the regulatory mechanisms for the expression of hsp90alpha and hsp90beta genes are different.  (+info)

Regulatory sequences of the mouse villin gene that efficiently drive transgenic expression in immature and differentiated epithelial cells of small and large intestines. (8/16045)

Villin is an early marker of epithelial cells from the digestive and urogenital tracts. Indeed villin is expressed in the stem cells and the proliferative cells of the intestinal crypts. To investigate the underlying molecular mechanisms and particularly those responsible for the restricted tissue specificity, a large genomic region of the mouse villin gene has been analyzed. A 9-kilobase (kb) regulatory region of the mouse villin gene (harboring 3.5 kb upstream the transcription start site and 5.5 kb of the first intron) was able to promote transcription of the LacZ reporter gene in the small and large intestines of transgenic mice, in a transmissible manner, and thus efficiently directed subsequent beta-galactosidase expression in epithelial cells along the entire crypt-villus axis. In the kidney, the transgene was also expressed in the epithelial cells of the proximal tubules but is likely sensitive to the site of integration. A construct lacking the first intron restricted beta-galactosidase expression to the small intestine. Thus, the 9-kb genomic region contains the necessary cis-acting elements to recapitulate the tissue-specific expression pattern of the endogenous villin gene. Hence, these regulatory sequences can be used to target heterologous genes in immature and differentiated epithelial cells of the small and/or large intestinal mucosa.  (+info)

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TLR2/NF-kB reporter cell line/TLR2 cell line can be used to monitor the TLR2 signaling pathway activity. Screen for activators or inhibitors of the TLR signaling pathway.
Reporter genes are fused to DNA fragments from a test gene or promoter. Reporter genes must be easily measured enzymatically or visually. Two common reporter genes are Β-galactosidase, green fluorescent protein (GFP) and yellow fluorescent protein (YFP). Β-gal is measured enzymatically using either X-gal (which Β-gal cleaves into a blue compound) or ONPG (which Β-gal cleaves into a yellow compound). GFP and YFP can be measured enzymatically, but are more easily measured visually because they fluoresce brightly.. ...
TY - GEN. T1 - Measurement of (anti-)oestrogenic potency in complex mixtures using a novel stably transfected luciferase reporter gene assay in the human T47D breast cancer cell. AU - Legler, J.. AU - van den Brink, C.. AU - Brouwer, A.. AU - van der Saag, P.. AU - Vethaak, A.D.. AU - Murk, A.J.. AU - van der Burg, B.. PY - 2000. Y1 - 2000. M3 - Conference paper. SP - 104. EP - 108. BT - Endocrine Disrupting Compounds : Wildlife and human health risks, The Hague 1998. CY - The Hague. ER - ...
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Regulation of gene expression can be achieved by fusing miRNA binding sites into the 3UTR of a GOI. In case a referring shRNA miR is endogenously present or co-expressed, the GOI is knocked down. Strength of regulation thereby depends on binding site properties. We are able to tune gene expression linearly over a broad range. This is a first proof of principle for various miRNA-mRNA interaction in vitro. Therefore, we transfected HeLa cells in principle with our pSMB_miTuner Plasmid HD3 . It turned out, that no obvious effect of different binding sites on reporter gene expression could be measured (data not shown). We assume that the RSV promoter driving the shRNA miR is too weak for tight regulation of the referring binding site behind the GOI. Only if a sufficient amount of shRNA miR binds to its target, translation is significantly repressed. Thus, we expressed the shRNA miR from a separate plasmid which was always co-transfected with the original tuning construct. The reporter genes - i. e. ...
Regulation of gene expression can be achieved by fusing miRNA binding sites right behind a GOI. In case a referring shRNA miR is expressed, the GOI is knocked down. Strength of regulation thereby depends on binding site properties. We are able to tune gene expression linearly over a broad range. This is a first proof of principle for various miRNA-mRNA interaction in vitro. Therefore, we transfected HeLa cells in principle with our pSMB_miTuner Plasmid HD3. It turned out, that there was no obvious effect of different binding sites on reporter gene expression (data not shown). We assume that the RSV driving the shRNA miR is too weak for tight regulation of the referring binding site behind the GOI. Only if a sufficient amount of shRNA miR binds to its target, translation is significantly repressed. Thus, we expressed the shRNA miR from a separate plasmid which was always co-transfected with the original tuning construct. The reporter genes - i. e. Luc2 and hRluc - were also expressed from ...
Alteration of the mouse genome through homologous recombination in embryonic stem (ES) cells is the most accurate and versatile way to dissect gene function in a vertebrate model. Most often, a selectable marker is used to create a knockout allele by replacing an essential part of the gene. However, …
motivation= We aim at providing a clean, simple interface that will be self-intuitive and will allow researchers to submit high-quality scientific findings directly to a well established central repository (WormBase). Data will be publically shared and could be mined alongside data curated from the literature. This will ensure that every piece of federally funded research will be publicly available -including negative findings. We will pilot the submission focusing initially only on reporter gene fusions -the most common method to detect gene expression in c.elegans (WormBook chapter in preparation), and plan to expand the model to other gene expression localization experiments (e.g. ISH, smFISH, immunohistochemistry). If the model will prove to be successful, we will scale it up to include submission of other scientific findings (laser ablation experiments, rnai experiments..). guidelines for gene product localization experiments assessed with reporter gene fusions. The guidelines will be ...
We aim at providing a clean, simple interface that will be self-intuitive and will allow researchers to submit high-quality scientific findings directly to a well established central repository (WormBase). Data will be publically shared and could be mined alongside data curated from the literature. This will ensure that every piece of federally funded research will be publicly available -including negative findings. We will pilot the submission focusing initially only on reporter gene fusions -the most common method to detect gene expression in c.elegans (WormBook chapter in preparation), and plan to expand the model to other gene expression localization experiments (e.g. ISH, smFISH, immunohistochemistry). If the model will prove to be successful, we will scale it up to include submission of other scientific findings (laser ablation experiments, rnai experiments..). guidelines for gene product localization experiments assessed with reporter gene fusions. The guidelines will be modeled after the ...
Reporter genes are genes whose products can be readily assayed subsequent to transfection and used as markers for screening successfully transfected cells.
Liam Payne has been accused of misunderstanding the true meaning behind LGBT Pride, after he featured in a new Adidas campaign. The former One Direction ...
The Fence Builders has recently opened its newest office and it promises to be the go-to place for all fencing needs. While being in the business for five years, the. ...
Today, the website of Mini PC News introduces the Best Gaming Mini PCs Under 300 Dollars 2017. The spokesman for this website says, The experts at budget Mini PCs are. ...
Easily study the dynamics of cellular processes with SBIs Cyto-Tracers™. Leverage SBIs high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors-available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The pCT-Mem-GFP (pCMV) Plasma Membrane Cyto-Tracer fuses a neuromodulin tag to copGFP, resulting in copGFP-labeling of the plasma membrane. The copGFP-fusion is expressed from the CMV promoter, for strong expression in common cell types such as HeLa, HEK293, and HT1080 ...
Easily study the dynamics of cellular processes with SBIs Cyto-Tracers™. Leverage SBIs high-quality lentivector technology to study subcellular localization and dynamics with our line of Cyto-Tracer vectors. Easily create stable reporter cell lines with our validated, pre-built lentivectors-available as transfectable plasmids or ready-to-transduce, pre-packaged virus. The ER-Golgi Cyto-Tracer, pCT-Secretory-GFP (CMV) fuses a secretory consensus tag to copGFP, resulting in copGFP-labeling of the ER and Golgi. The copGFP-fusion is expressed from the CMV promoter, for strong expression in common cell types such as HeLa, HEK293, and HT1080 ...
Jill Abramson, executive editor of the New York Times, gave a frank overview of the evolving role of the newsroom as she described how the Times is blending traditional separations between print and web ...
Comment on attachment 573750 [details] [diff] [review] patch ,-// There are two kinds of TreeNode. Those that correspond to Reporters ,-// have more properties. The remainder are just scaffolding nodes for the ,-// tree, whose values are derived from their children. ,+// There are two kinds of TreeNode. ,+// - Leaf TreeNodes, that correspond to Reporters and have more properties. ,+// - Non-leaf TreeNodes are just scaffolding nodes for the tree; their values ,+// are derived from their children. Nit: Parallelism. Non-leaf TreeNodes, *which* are just scaffolding ,@@ -413,28 +420,26 @@ TreeNode.compare = function(a, b) { , * @return The built tree. , */ , function buildTree(aReporters, aTreeName) , { , // We want to process all reporters that begin with ,aTreeName,. First we , // build the tree but only fill the properties that we can with a top-down , // traversal. , ,- // Is there any reporter which matches aTreeName? If not, well create a ,- // dummy one. ,+ // Is there any reporter which ...
The LightSwitch 3´UTR Reporter Collection from Active Motif include over 12,000 3´UTRs available as transfection-ready luciferase reporter vectors for use in reporter assays.
Background Esophageal tumor (EC) is one of the deadliest cancers worldwide. protein levels. A dual-luciferase reporter gene assay was used to set up the interactions among circRNA-0008717, miR-203, and Slug. Results circRNA-0008717 expression was significantly upregulated in EC cells, and miR-2031 expression was decreased. Moreover, si-circRNA-0008717 or si-Slug inhibited the proliferation, migration, and invasion of EC cells. We found that circRNA-0008717 functioned as a sponge of miR-203, resulting in increased expression of Slug. We also reversed the effect of circRNA-0008717 knockdown on the EC progression by co-transfecting EC cells with a miR-203 inhibitor or Slug. Conclusions The proliferation, invasion, and migration of EC cells were enhanced by circRNA-0008717 sponging the miR-203 to increase Slug expression. was used to normalize the transcript levels of circRNA-0008717 and Slug. Relative expression is calculated using the 2-Ct method (24). Western blot analysis Total protein was ...
We were contacted recently with the following request which we are unable to fulfill:- ...................................................................... Hi, I am studying cis-regulation of a gene encoding nuclear protein. I am using beta-gal to show the expression pattern of this gene. But the result is not so good because beta-gal is expressed in cytoplasm, not nuclearly. Do you know any nuclear reporter gene I can use? Thank you very much! Zhe Han zhan at umich.edu ...................................................................... If you are able to help please contact Zhe directly. Corniche.. the On-line life science resource. Free to use searchable database conatining 50,000 products from many sources large and small. http://www.corniche.com ...
"Reporter genes have been used for several decades to study regulation of gene... *Hitra in zanesljiva dostava, plačilo tudi po povzetju.*
The behavior of single cells within a metabolic cycling population was visualized using phase-specific fluorescent reporters. The reporters largely recapitulated genome-specified mRNA expression profiles. A significant fraction of the cell population appeared to exhibit basal expression of the repor …
rhAmp SNP Master Mix includes unique Taq and RNase H2 enzymes. Reporter Mixes contain the universal reporter probe mix for signal generation in rhAmp SNP genotyping.
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
Our comprehensive collection contains more than 12,000 endogenous human 3′UTR reporter GoClone® constructs that are transfection-ready with no DNA preparation required. Our reporters contain a novel luciferase gene for industry-leading sensitivity ...
Deport the Middletons to Sandwich Islands: campaign gathers momentum : London A TV presenters quip about deporting problem families to the far flung archipelago has morphed into an exciting new project Sky News reporter Colin Braziers suggestion about sending 120000 miscreant families each costing the taxpaye
CBS Evening News reporter Steve Hartman took a deeper look into how his two young kids were computing the lyrics of country songs in their developing brains as
Project no. 911388 outlined 3 major aims to be addressed; Development of novel optical reporter systems, implementation of these reporters and probes in optical imaging of cancer and in translational therapeutic imaging. Results are very encouraging with publications resulting in prestigious journals. Gjerdrum et al. (2010) employed the use of novel imaging the impact of shRNA knockdown in vivo, while novel time-domain technique was described to image fluorescent reporters in vivo (Rosland et al. 2009, Torsvik et al. 2009). Combination of novel imaging strategy and near-infrared labeled mAbs and dextrans permitted visualization of drug uptake (McCormack et al. 2009), while Wang et al. (2010), Karlsen et. al. (2012) and 2 further manuscripts (McCormack et al. Submitted 2011/2) detail novel imaging strategies and reporters for in vivo imaging of patient xenografts. Translational application of cancer models and imaging are detailed in Erikstein et al. (2010), McCormack et al. (2010), and in ...
PrimCells is the reliable choice for your primary cell needs. We carry a variety of high quality primary cells, reporter cell lines and protein factors. Our uniquely design molecular tools provide you the most cost effective and reliable tool sets that can empower your research.
EPA-induced translational up-regulation of reporter genes fused to BRCA1 mRNAb 5′UTR is dependent on the presence of tandem uORFsThe plasmid encoding for F-lu
GSK3β inhibitors are one of the few drugs identified in this screen that are capable of inhibiting EMT a Schematic of reporter system in MDA MB 231 reporter cells that were used to screen a panel of smallmolecule drugs In the assay cells that have a mesenchymallike phenotype express GFP green and those with epithelial cells express RFP red. Get Price ...
Available with Biological Response Genes AdenoExpress™ Available with Biological Response Genes Cytokines GM-CSF IFN-a IFN-g IL-2 IL-12
EXPRESS: If we want to use the regulatory aspects of NBB4 ethylene metabolism in a biosensor, we need to incorporate a reporter gene with strong, detectable output into our circuit. Generating such a reporter gene was the focus of EXPRESS, where we used error prone PCR on an existing blue iGEM chromoprotein amilCP to generate amilCP mutants with altered spectral characteristics ...
Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro ...
E2HSA exhibits GLP-1 receptor activating efficacy in NIT-1 cells. NIT-1 cells transiently transfected with Peak12 RIP-CRE 6x Luciferase reporter gene plasmid we
Community testing a must, she says State Rep. Liz Miranda is on the mend after testing positive for COVID-19 on May 1. The 39-year-old lawmaker, who has represented the 5th Suffolk District since Jan. 2019, talked to the Reporter this week about her experience with the virus, health disparities in Roxbury and Dorchester, and the importance of community testing. Of the 20 of
Amused at a couple of things in the Glen Rose Reporter article of June 2, 2016 that deals with Luminants not having paid the entire property tax ....
Given there was another eye-witness who wasnt arrested, I would guess that the area wasnt secure and was open for others to use, so that at least would be out as far as justifications for the arrest.. The statement made by that eyewitness would also seem to contradict the aggressive narrative that the arrest was based on, though its possible that the SS had a different idea as to what constituted too close that differed from the eyewitness and the arrested reporters.. The fact that yelling questions was categorized as causing a disturbance is all sorts of questionable on its own, and the fact that it was included in the complaint does not bode well for the rest of the thing, especially given the other two points above. ...
An honest and intelligent reporter should ask Dr. Fauci these key questions about COVID-19, questions that show why we need to lift lockdowns.
Hottie Mexican reporter Ines Sainz described New York Jets catcalls as she was working on a story in their locker room as grotesque. It mustnt happen again, she told the New... Sports News Summaries. | Newser
TY - JOUR. T1 - Coexpression of herpesviral thymidine kinase reporter gene and VEGF gene for noninvasive monitoring of therapeutic gene transfer. T2 - An in vitro evaluation. AU - Anton, Martina. AU - Wittermann, Constanze. AU - Haubner, Roland. AU - Simoes, Marcus. AU - Reder, Sybille. AU - Essien, Bryan. AU - Wagner, Bettina. AU - Henke, Julia. AU - Erhardt, Wolf. AU - Noll, Steffi. AU - Hackett, Neil R.. AU - Crystal, Ronald G.. AU - Schwaiger, Markus. AU - Gansbacher, Bernd. AU - Bengel, Frank M.. PY - 2004/10/1. Y1 - 2004/10/1. N2 - Coexpression of a reporter gene and a therapeutic gene may allow for noninvasive monitoring of cardiac gene therapy. We sought to evaluate the usefulness of an adenoviral vector expressing mutant herpesviral thymidine kinase reporter gene (HSV1-sr39tk) and vascular endothelial growth factor (VEGF) 121 in independent expression cassettes (Ad4tk). Methods: Accumulation of 14C-2′-fluoro-5-methyl-1-β-D-arabinofuranosyluracil (FIAU) and ...
OV-IA82Δ113 and OV-IA82Δ116 viruses were constructed by infecting OFTu cells (in T25 flasks) at a multiplicity of infection (MOI) of 1.0 with wild type OV-IA 82 for 3 hours and subsequently transfecting the cells with 10 μg of pSVP-113LF-EGFP-113RF and pSVP-116LF-EGFP-116RF transfer vectors by standard in vivo recombination protocols [28, 29]. Transfections were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions.. Viruses were harvested 48 h pi by scraping infected/transfected OFTu cells into sterile 15 ml conical tubes. The cell suspensions were vortexed, frozen/thawed 3 times, and then centrifuged at 1000 rpm, for 10 min at 4°C (Eppendorf Centrifuge 5810R, 15 amp version, Hamburg, Germany). The supernatant (viruses) were transferred into 2 ml cryogenic vials (Corning, NY, USA) and stored at -80°C for future use.. In order to select and purify recombinant viruses, limited dilution and plaque assays were performed. 3 ×104 of ...
The stimulation of reporter gene expression following co-transfection with the S4 gene of mammalian reoviruses was analysed. The σ3 protein of type 3 reovirus gave a five- to eightfold increase in expression of chloramphenicol acetyltransferase and β-galactosidase but this was found to be dependent upon the nature of the promoter being used to drive reporter gene expression. The σ3 protein of reovirus type 1 failed to stimulate reporter gene expression under any of the conditions used. Hybrid constructs between the S4 genes of reoviruses type 1 and 3 were used to map the stimulation characteristic to the carboxy-terminal third of the gene. Analysis of the level of σ3 protein accumulation in transfected cells showed that the reovirus type 3 protein accumulated to a much higher level than that of reovirus type 1. Using the hybrid gene constructs this higher level of protein accumulation was shown to co-segregate with the ability to stimulate reporter gene expression.
Reporter genes are a well-established method used to develop cell-based assays for testing drugs that inhibit targets involved in specific signaling pathways. Here, we introduce new PathHunter® Signaling Pathway Reporter Assays that utilize the industry-validated Enzyme Fragment Complementation (EFC) technology to detect reporter gene activity. Reporter cells with endogenous or heterologously introduced target receptors are used to readout signaling pathway activation, resulting in transcriptional activation of a reporter gene encoding a protein tagged with a small enzyme fragment. Reporter gene activity is measured by the addition of lysis buffer, luminescent enzyme substrate and the complementary larger enzyme fragment in an easy-to-use format. For example, an NF-κB reporter assay was developed and validated with CD40L to measure endogenous CD40 activation in U2OS cells. Another assay developed is the PathHunter Jurkat NFAT Pathway Reporter cell line, which measures T-cell activation but has ...
Signaling through the Notch1 receptor is essential for the control of numerous developmental processes during embryonic life as well as in adult tissue homeostasis and disease. Since the outcome of Notch1 signaling is highly context-dependent, and its precise physiological and pathological role in many organs is unclear, it is of great interest to localize and identify the cells that receive active Notch1 signals in vivo. Here, we report the generation and characterization of a BAC-transgenic mouse line, N1-Gal4VP16, that when crossed to a Gal4-responsive reporter mouse line allowed the identification of cells undergoing active Notch1 signaling in vivo. Analysis of embryonic and adult N1-Gal4VP16 mice demonstrated that the activation pattern of the transgene coincides with previously observed activation patterns of the endogenous Notch1 receptor. Thus, this novel reporter mouse line provides a unique tool to specifically investigate the spatial and temporal aspects of Notch1 signaling in vivo. ...
The assessment of how many cells have been successfully transfected or transduced in a cell population is a basic and critical evaluation parameter in many cell and molecular biology labs. Commonly, the cells of interest are transfected or transduced with a construct that results in the expression of a fluorescent protein (FP) reporter, such as GFP.
BioAssay record AID 95279 submitted by ChEMBL: Cellular activity was measured by an IL-2 luciferase reporter gene assay on a Jurkat human T cell line activated by anti-CD28 and anti-TCR antibodies.
The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay allows you to use firefly luciferase, Nanoluc luciferase, or both, as primary reporters. This article provides information on when to use firefly and when to use Nanoluc luciferase as a primary reporter, and how both reporters can be used together to measure two biological responses in the same sample.
riyer at rascal.med.harvard.edu wrote: : I recd. so many requests from people who did not get my earlier post that I : decided to save time by re-posting it. At the outset I would like to state : that I am only a satisfied user of the above vectors and am not associated : with the company that markets them. And now here comes a readable version (with ,80 chars/line) :-) : Hi netters, I would like to report the availability of a set of : reporter gene vectors that have some distinct advantages over those : currently in use for the purpose of analysis of eukaryotic promoters : & enhancers. The vectors , SV40-Syncat, Syncat I, Syncat II, : SV40-pFlash, pFlash I and pFlash II, are as their names suggest : CAT & Luciferase (Luc) reporter gene vectors. What distinguishes : them from other vectors in this category is that these vectors produce : near-zero background. This feature is due to the fact, that they use a : modified SV40-t-intron as the donor for the poly-adenylation signal. : The wild type ...
A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple
The synthetic poly(A) signal in the pCAT3 vector and several other Promega vectors is placed upstream of the reporter to eliminate background and vector dependent reporter signal. Basically, all vectors have cryptic transcription factor binding sites that will cause the reporter to be transcribed. By putting in a polyadenylation signal, these transcripts should no longer contain the reporter gene because it is spliced off and will not cause artificial signal. I hope this is clear. If you have any additional questions, please feel free to contact me at [email protected] ...
Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gene reporter constr
Anew detection mechanism for the control of successful siRNA delivery to cells or tissue is introduced using a siRNA-based probe that is capable of inducing a ...
Thermo Scientific™ Gaussia-Dura Luciferase Reporter Assay Vectors pTK-Gaussia-Dura Luc Vector Thermo Scientific™ Gaussia-Dura Luciferase Reporter...
Data showing positive regulation of Wnt/β-catenin signal transduction by FAM129B siRNA using transcriptional reporter assay, target gene expression, and apoptosis assay
The EliKine™ Human IL-1β ELISA Kit is one of the latest scientific research products from Abbkine Scientific Co. Ltd. The company recently announced the launch of the product, designed to enhance scientific research processes.. The IL-1β is a member of the interleukin 1 cytokine family, produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). Interleukin 1β is an important mediator of the inflammatory response, involved in several cellular activities, including cell proliferation, differentiation, and apoptosis.. Interleukin 1β Elisa kit has human reactivity, employing a two-site sandwich ELISA to quantitate IL-1β in samples. The IL1B Elisa kit uses colorimetric method of detection, with the suitable samples types being Cell culture supernatants, other biological fluids, Plasma, and Serum.. The components of the kit include:. ...
Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine ...
The Global CM & CRM Devices Sales Market Report 2021 is a professional and in-depth study on the current state of the CM & CRM Devices Market. This report studies sales (consumption) of CM & CRM Devices in Global market, especially in North America, Europe, China, Japan, Southeast Asia and India, focuses on top players in these regions/countries, with sales, price, revenue and market share for each player in these regions, covering North America, Europe, China, Japan, Korea, Taiwan focuses on top manufacturers in global market, with production, price, revenue and market share for each manufacturer, covering Medtronic PLC , St. Jude Medical, Inc. , Boston Scientific Corporation , GE Healthcare , Philips Healthcare , Biotronik Se & Co., Kg , Cardiac Science Corporation , Livanova PLC , Mortara Instrument, Inc. and Schiller AG.. Request a Sample copy of this research @ http://www.reportsweb.com/inquiry&RW0001513144/sample. Market Segment by Regions, this report splits Global into several key ...
Dc3 is a carrot lea-class gene belonging to a small gene family that encodes Dc3 and Dc3-like RNA sequences. We have examined the responsiveness to water deficit and abscisic acid (ABA) of the promoter/enhancer complex of Dc3 fused to a beta-glucuronidase (GUS) reporter gene in vegetative cells of transgenic tobacco. In 56-d tobacco, GUS expression in leaves increased about 200-fold during a 3-d d ...
Currently about 1,500,000 people die of AIDS and its complications each year. Many die of fungal infections - how many is not clear because of a lack of access to excellent diagnostic tests.. In Seattle, over 60 experts from major health agencies, including the World Health Organisation, UNAIDS, Bill and Melinda Gates Foundation, Clinton Health Access Initiative, Office for Global AIDS Coordination, Centers for Disease Control The Global Fund - and representatives from over 30 countries -- gathered to align on efforts to improve management of fungal infections. Delegates highlighted the lack of diagnostics and access to treatments, including the oldest antifungal drugs, as major hurdles to improvements in many developing countries.. A key output of the meeting based on a preliminary model created to analyze the costs associated with treatment and care of fungal infections in Africa, showed that early screening and treatment, before meningitis or pneumonia develop, is cost effective. If ...
Humorous views on interesting, bizarre and amusing articles, submitted by a community of millions of news junkies, with regular Photoshop contests.
Humorous views on interesting, bizarre and amusing articles, submitted by a community of millions of news junkies, with regular Photoshop contests.
Inverted repeats, or reverse complementary nucleotide sequences, can base pair with one another to form hairpin or cruciform structures that can stall replication fork progression and cause DNA damage. Inappropriate DNA repair events at such stalled replication sites could lead to fusion of inverted repeats and subsequently generate chromosomal rearrangements. To gain insight into the mechanisms underlying inverted repeat fusion, Hu and colleagues created reporter cassettes in which fusion of identical or mismatched inverted repeats would allow survival on selection media. Fusion of the identical and mismatched repeat reporters (IRR and MRR) occurred spontaneously in wild-type murine embryonic stem (ES) cells and led to unstable and complex chromosome rearrangements. DNA damaging agents increased fusion of the reporters, but γ-irradiation selectively induced IRR fusion and ultraviolet light selectively induced MRR fusion, suggesting that different pathways mediate inverted repeat fusion ...
Reporter gene assays are paramount for study of regulation of gene expression by gene regulatory elements (cis-acting factors), transcription factors or exogenous regulators (trans-acting factors).
Optical imaging of whole, living animals has proven to be a powerful tool in multiple areas of preclinical research and has allowed noninvasive monitoring of immune responses, tumor and pathogen growth, and treatment responses in longitudinal studies. However, fluorescence-based studies in animals are challenging because tissue absorbs and autofluoresces strongly in the visible light spectrum. These optical properties drive development and use of fluorescent labels that absorb and emit at longer wavelengths. Here, we present a far-red absorbing fluoromodule-based reporter/probe system and show that this system can be used for imaging in living mice. The probe we developed is a fluorogenic dye called SC1 that is dark in solution but highly fluorescent when bound to its cognate reporter, Mars1. The reporter/probe complex, or fluoromodule, produced peak emission near 730 nm. Mars1 was able to bind a variety of structurally similar probes that differ in color and membrane permeability. We ...
We show proof-of-principle examples that illustrate the user friendliness, speed, versatility, and genetic power of the MuLE system in primary mammalian cells. Complex genetic modulations involving combinatorial gene overexpression, knockdown, and knockout can be achieved using single viral infections. The simultaneous marking of infected cells with a variety of reporter cassettes facilitates cellular studies and in vivo imaging studies. By expressing multiple sgRNAs together with hCas9, single MuLE vectors are able to simultaneously target genetic mutations to multiple loci, providing a platform that harnesses the genetic power of the CRISPR/Cas9 system for performing combinatorial genetic manipulations. Combinatorial genetic screening experiments are also possible by combining libraries of Entry vectors with Entry vectors that encode defined genetic alterations. The ease of combining different genetic elements using this system represents a tool that will greatly facilitate systematic ...
Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins are encoded on a single transcript but are separated during translation by a 2A
High throughput (HTS) and high content screening (HCS) assays often utilize cell sources, which are not amenable to large-scale screening and can require extensive cell culture and processing prior to imaging. To overcome this hurdle, Hera and a commercial partner ArunA Biomedical, engineered human neural progenitors derived from stem cells with a non-viral vector encoding a GFP reporter gene using a selectable piggyBac™ gene editing system to produce hNP1 GFP+ cells. hNP1 reporter cells exhibit detectable dose dependent response to toxins tested, (cytotoxicity and cell migration) providing a sensitive, high throughput and high content amenable, cell based human neurotox assay platform.. ...
There was a positive correlation between CEA levels and CA 15-3 levels and patient prognosis. A high specificity for B-cells was confirmed by assaying for luciferase reporter gene expression from a plasmid containing an analogous combination of immunoglobulin heavy chain regulatory ...
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: 05-12-17 Dunwoody Reporter, Author: Reporter Newspapers, Name: 05-12-17 Dunwoody Reporter, Length: 24 pages, Page: 1, Published: 2017-05-11
Issuu is a digital publishing platform that makes it simple to publish magazines, catalogs, newspapers, books, and more online. Easily share your publications and get them in front of Issuus millions of monthly readers. Title: JULY 2019 - Buckhead Reporter, Author: Reporter Newspapers, Name: JULY 2019 - Buckhead Reporter, Length: 32 pages, Page: 1, Published: 2019-06-19
An American news reporter gathering a story about the work of the Sisters of Charity in Calcutta asked one of the sisters where he might find Mother Teresa. The nun smiled for a moment, considering this Westerners question, then answered, You will find her with the poor. The reporter, trying to get to a more helpful, definitive response, then asked, Well, where might I find the poor? The nun answered, again with a bit of a smile, Why, they are everywhere. All about you. Cant you see them. Now frustrated, the reporter pressed on, Yes, yes, I know she works with the poor. But WHERE is Mother Teresa working with the poor? The nun - possibly with a bit of delight -, then answered, Wherever the poor might be found this morning! She paused for a moment then offered her hand to the reporter, Come, let us both go looking for some poor people ...
Microarray analysis and RNA-Seq are powerful tools for investigating differential gene expression in animal models of human genetic disease. Often, these models are loss of function mutations introduced by gene targeting or trapping. One difficulty in such experiments, especially where work on congenital malformations is concerned, is that whole embryo or regional dissections will commonly contain cells that do not express the gene of interest. Thus where cell autonomous effects are of primary interest, for example in the case of transcriptional regulators, such experiments will be compromised by dilutional effects and perhaps secondary effects on surrounding, non expressing tissues. Isolation of the relevant expressing lineage can mitigate these effects. Flow cytometry offers one route to cell purification but requires a fluorescent marker, and relatively few fluorescent protein gene reporters are available. FACS-Gal (1) offers the potential to capitalize upon the large existing repository of ...
BMG LABTECHs Microplate Readers have received Wavelength Based Dual Glow Reporter Genes certification for their outstanding performance. Read more!
HEK-Blue™ Null2 cells express the SEAP reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-kB and AP-1 binding sites. HEK-Blue™ Null2 is the parental cell line of several HEK-Blue™ TLR and HEK-Blue™ NOD cells.
BioAssay record AID 683120 submitted by ChEMBL: Inhibition of human ALK5 activity in human HaCaT cells expressing p3TP-luciferase reporter construct assessed as TGFbeta-induced p3TP-luciferase reporter activity at 30 nM relative to control.
"Gene Deitch". lambiek.net. Retrieved May 5, 2020. Weiner, Tim (May 9, 2020). "Little Richard, Flamboyant Wild Man of Rock 'n' ... The Hollywood Reporter, April 2, 2020. "James Bond and The Witches actor Vincent Marzello has died". The Independent. March 31 ... April 16: Gene Deitch, American comics artist, animator and animated film director (Munro, Tom Terrific, Nudnik, continued the ... "Ron McLarty, Actor on 'Spenser for Hire,' 'Cop Rock' and 'Law & Order,' Dies at 72". The Hollywood Reporter. Retrieved May 5, ...
Robertson, Gene. "On the Beam" (film news and reviews). San Francisco Sun Reporter, Jul. 10, 1971. Dickinson, pp. 27-33 Del ...
2021 issue of The Hollywood Reporter magazine. Maddaus, Gene; Maddaus, Gene (April 19, 2021). "Friends of Scott Rudin's Late ... "Scott Rudin Dresses Down NY Times Theater Reporter". The Hollywood Reporter. Retrieved February 28, 2019. Healy, Patrick (May 1 ... Following the Hollywood Reporter allegations, Rudin announced that he would be "stepping back" from his Broadway, film, and ... In 2013, after New York Times theatre reporter Patrick Healy published an interview with Colm Toibin, the author of Rudin's ...
"Gene Packard". Society for American Baseball Research. Retrieved 24 March 2016. CS1 maint: discouraged parameter (link) " ... "Packard Breaks World's Record". Independence Daily Reporter. August 10, 1908. v t e. ... The most notable player on the 1908 Jewelers team was pitcher Gene Packard. While pitching for Independence against Tulsa, ...
"Curse of the Golden Flower". The Hollywood Reporter. Archived from the original on February 5, 2009. Gene Seymour. "Curse of ... Kirk Honeycutt of The Hollywood Reporter wrote the film is "A disappointing misfire from a great director." Gene Seymour of ...
"Announcer Gene Rayburn". American Profile. 2003-10-05. Retrieved 2018-11-06. "Willard Scott - Weather Reporter and Centenarian ... The Hollywood Reporter. Retrieved April 11, 2018. Steinhauser, Si (8 May 1935). "Radio Chiefs Train Studio Guides For Bigger ... Today Show executive producer Don Nash TV personality Regis Philbin Actress and comedian Aubrey Plaza Match Game host Gene ...
"Advantages of firefly luciferase as a reporter gene: application to the interleukin-2 gene promoter". Analytical Biochemistry. ... Massoud TF, Paulmurugan R, De A, Ray P, Gambhir SS (Feb 2007). "Reporter gene imaging of protein-protein interactions in living ... Luciferases are widely used in biotechnology, for microscopy and as reporter genes, for many of the same applications as ... "Trends in development of reporter genes". reportergene.com. Retrieved 2009-03-07. "BL Web: Luciferin types". The ...
Demby, Gene. "George Lucas: Hollywood Didn't Want To Fund 'Red Tails' Because Of Its Black Cast." huffingtonpost.com, January ... Fernandez, Jay A. "Director picked for Lucasfilm project." The Hollywood Reporter, September 30, 2008. "George Lucas says ...
Hawaii Reporter Inc. March 7, 2013. Retrieved 22 October 2017. CS1 maint: discouraged parameter (link) Johnson, Mark; Dembrow, ... Michael; McLane, Mike; Vega Pedersen, Jessica; Whisnant, Gene; Williamson, Jennifer; Hansell, Bill; Thomsen, Chuck (29 May 2013 ... Hawaii.gov Office of Elections "Hawaiian Bat and Microbe Take Center Stage at State Legislature". Hawaii Reporter. ...
Phillips, Gene (2006). Beyond the Epic: The Life and Films of David Lean. p. 383. ISBN 0813171555. Barnes, Mike (February 1, ... 2014). "'Ryan's Daughter' Star Christopher Jones Dies at 72". The Hollywood Reporter. Retrieved February 1, 2014. CS1 maint: ...
ISBN 978-1-4000-4728-4. Conason, Joe; Lyons, Gene (2001). The Hunting of the President: The Ten-Year Campaign to Destroy Bill ... ISBN 978-0-312-27319-4. Isikoff, Michael (1999). Uncovering Clinton: A Reporter's Story. New York: Crown Publishing Group. ISBN ...
"State Senate District 27, KS". Census Reporter. Retrieved August 19, 2020. "Senator Gene Suellentrop". Kansas State Legislature ... It has been represented by Republican Gene Suellentrop since 2017, succeeding fellow Republican Leslie Donovan. District 27 is ...
"James Carville". Anchors/Reporters. CNN. 2009. Retrieved July 1, 2013. Often referred to as the "Ragin' Cajun" for his animated ... Schneider, Bridgett Edwards (1995-2000). "A Little History of Tennessee". Bridgett's Gene Joint. MomNDoc Online Services. ... reporter for The Wall Street Journal (1977), the Washington Star (1978), The Bergen Record (1978-1979), Chicago Sun-Times (1979 ... Hall of Fame former second baseman in Major League Baseball Gene Lamont, former catcher and who managed the Chicago White Sox ( ...
... through use as a reporter gene. Luciferase was first purified by McElroy and Green in 1955. It was later discovered that there ... Despite this common gene organization, variations, such as the presence of other lux genes, can be observed among species. ... The luxA and luxB genes encode for the α and β subunits, respectively. In most bioluminescent bacteria, the luxA and luxB genes ... All bioluminescent bacteria share a common gene sequence: the lux operon characterized by the luxCDABE gene organization. LuxAB ...
Phillips, Gene D. (1999). Major Film Directors of the American and British Cinema. Lehigh University Press. p. 122. ISBN ... "Oscar-winning actor Paul Scofield dies". The Hollywood Reporter. Reuters, AP. 21 March 2008. Retrieved 26 October 2020. Bergman ...
Lang, Brent; Maddaus, Gene; Setoodeh, Ramin (October 4, 2017). "Harvey Weinstein Lawyers Up for Bombshell New York Times, New ... Vlessing, Etan (May 21, 2019). "Benedict Cumberbatch's 'The Current War' to Get Domestic Release". The Hollywood Reporter. ... The Hollywood Reporter. Retrieved October 17, 2018. Fleming Jr, Mike (April 2, 2019). "Sparked By A Martin Scorsese Contract ... David Rooney for The Hollywood Reporter wrote, "For all its aggressive energy, The Current War is an uninvolving bore, making ...
The Hollywood Reporter. Retrieved 14 March 2018. Siskel, Gene (August 8, 1974). "'Pink Floyd' and Pompeii". Chicago Tribune. ... Gene Siskel of the Chicago Tribune gave the film three stars out of four and wrote, "The interviews with Pink Floyd are too ... The Hollywood Reporter called it a "fully structured concept which stands on its own quite beyond its function of recording a ...
The Hollywood Reporter. Archived from the original on October 17, 2017. Retrieved October 17, 2017. Maddaus, Gene (October 24, ... Maddaus, Gene (October 9, 2017). "Harvey Weinstein Hires Sitrick and Company to Handle Crisis Response After Firing". Variety. ... Maddaus, Gene; Wagmeister, Elizabeth; Nichols, Mackenzie (February 5, 2020). "Jessica Mann Is Raw, Wounded, and Angry. Will Her ... Maddaus, Gene (July 14, 2020). "Judge Rejects Harvey Weinstein Settlement: 'I Can't Subscribe to That'". Variety. Retrieved ...
Colter, Gene (13 May 2005). "Sin, Revisited". New York: The Wall Street Journal. Staff writer (1 September 2004). "SRI with an ... "SRI with an Active Twist". Research Reporter. Retrieved 12 May 2005. Wine, Elizabeth. "SRI Plows the Path to Profitability". On ... reporter for On Wall Street magazine, noted that Advocacy Investing has become the new generation of socially responsible ... Active Twist". Research Reporter. Retrieved 12 May 2005. Write, Staff. " ...
Maddaus, Gene (April 11, 2017). "Snapchat CEO Said 'This App Is Only for Rich People,' Ex-Employee Alleges". Variety. Penske ... "Snap's Board Facing Blowback for Not Disclosing Whistle-Blower Lawsuit in IPO". The Hollywood Reporter. Retrieved March 12, ... Maddaus, Gene (April 11, 2018). "Judge Shuts Down Snapchat Whistleblower's Lawsuit". Variety. Retrieved March 12, 2020. " ...
Gene Siskel was more critical, describing the film as "one extremely violent, protracted attack on the senses ... Some people ... Weiner, David (May 24, 2019). "Ridley Scott On The Hard Road To 'Alien'". The Hollywood Reporter. Archived from the original on ... Siskel, Gene (August 8, 1986). "Flick Of Week: 'Vagabond' One Of Finest Films In Years". Chicago Tribune. Archived from the ... Dennis Fischer wrote for The Hollywood Reporter, however, the unrelenting scenes of action and suspense worked for Aliens as ...
The Hollywood Reporter. Retrieved September 24, 2017. Maddaus, Gene (September 25, 2017). "Ain't It Cool News Contributors Step ... "Ain't It Cool's Harry Knowles: The Cash-Strapped King of the Nerds Plots a Comeback". The Hollywood Reporter. Retrieved ... "Alamo Drafthouse Severs Ties With Harry Knowles Over Sexual Assault Allegations". The Hollywood Reporter. September 25, 2017. ... The Hollywood Reporter. Erbland, Kate (September 21, 2017). "Ain't It Cool News Dropped as Fantastic Fest Sponsor". IndieWire. ...
"Global Road Files Chapter 11 Bankruptcy for Film Division". The Hollywood Reporter. Retrieved 2018-09-07. Maddaus, Gene ( ... Holdsworth, Nick (December 27, 2006). "Disney, Sony team up for Russian content". The Hollywood Reporter. AP. Retrieved June 13 ... Weprin, Alex (December 4, 2019). "Bob Bakish's Memo to ViacomCBS Staff: Merger "A Historic Moment"". The Hollywood Reporter. ... Ng, Philiana (2011-04-15). "Paramount Vantage Sets Release Date for Sundance Winner 'Like Crazy'". Hollywood Reporter. ...
Genes are turned on and off in response to these events, as epigenetic marks are gradually layered above genes, etching the ... The Key Reporter. Mukherjee, S. (2 May 2016). "Same but different". The New Yorker. Retrieved 8 May 2017. Mukherjee, S. (17 May ... 2016: Washington Post's "10 Best Books of 2016" for The Gene. 2017: Phi Beta Kappa Society Book Award in Science for The Gene. ... His 2016 book The Gene: An Intimate History made it to #1 on The New York Times Best Seller list, and was among The New York ...
Maddaus, Gene (2019-02-21). "Michael Jackson Estate Sues HBO for Non-Disparagement Contract Breach". Variety. Retrieved 2019-09 ... "Bad 25: Venice Review". The Hollywood Reporter. Retrieved 2017-12-13. "Michael Jackson's Estate and Michael Jackson Archives". ... Maddaus, Gene (2019-09-19). "Judge Sides With Michael Jackson Estate in 'Leaving Neverland' Dispute". Variety. Retrieved 2019- ... Gene Maddaus,"HBO Appeals Arbitration Order in 'Leaving Neverland' Fight", Variety, October 21, 2019. Retrieved November 7, ...
Siskel, Gene (January 25, 1997). "The Joy of Watching 'The Joy Luck Club'". TV Guide: 18. Siskel, Gene (February 14, 1997). "A ... McClintock, Pamela (August 19, 2011). "Why CinemaScore Matters for Box Office". The Hollywood Reporter. Retrieved August 9, ... Siskel and Ebert' Top Ten Films (1980-1998): 1993". Gene Siskel: The Official Site. Estate of Gene Siskel. Archived from the ... Critic Gene Siskel, singled out the script and performances, praising the film for presenting images of Asian-Americans outside ...
Demby, Gene (January 30, 2013). "Boy Scouts' Repeal Of Gay Ban Mirrors Its Approach To Racial Integration". NPR. Retrieved ... "Black Boy Scout Ban Threatens Church". Observer-Reporter. September 11, 1974. Retrieved April 29, 2013. "NAACP Sues Boy Scouts ...
Maddaus, Gene (March 2, 2018). "David Bergstein Convicted of Defrauding Investors Out of $26 Million". Variety. Retrieved June ... "Producer David Bergstein Arrested in $26M Fraud". The Hollywood Reporter. Associated Press. November 9, 2016. Retrieved June 27 ...
Gene Siskel gave the film two-and-a-half stars out of four and wrote, "Sorry to say this, but there's not anything new in Rocky ... "Why CinemaScore Matters for Box Office". The Hollywood Reporter. Archived from the original on 2014-04-26. Retrieved 2018-05-09 ... Roger Ebert and Gene Siskel attributed the film's success to the positive reaction from critics and audiences towards Rocky II ... Siskel, Gene (May 28, 1982). "'Rocky' loses its punch in Round 3". Chicago Tribune. Section 3, p. 3. Kael, Pauline (May 31, ...
The Hollywood Reporter. Neil Marshall, Charlotte Kirk Fiancé, Dropped By Verve Agency - Deadline, 27 August 2020 Maddaus, Gene ... Maddaus, Gene (20 August 2020). "Director Neil Marshall Cancels 'The Reckoning' Q&A Amid Ron Meyer Firestorm". Variety. ... In September 2020, The Hollywood Reporter revealed that Tsujihara and Meyer may have colluded to cover up the real nature of ... CS1 maint: discouraged parameter (link) "The Legal Maze Behind a Charlotte Kirk Movie About O.J. Simpson". Hollywood Reporter. ...
In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to " ... Huang J, Ru B, Li S, Lin H, Guo FB (2010). "SAROTUP: scanner and reporter of target-unrelated peptides". J. Biomed. Biotechnol ... A versatile screening system for selective isolation of genes by specific gene-product/ligand interaction". Eur. J. Biochem. ... Parmley SF, Smith GP (1988). "Antibody-selectable filamentous fd phage vectors: affinity purification of target genes". Gene. ...
Railroad and Transportation Museum of El Paso The Gene Roddenberry Planetarium Lynx Exhibits Ysleta Mission is recognized as ... "Census profile: El Paso, TX Metro Area". Census Reporter. Retrieved April 19, 2021. "Juarez Outlook 2017, Desarrollo Economico ... "The Gene Roddenberry Planetarium -". grplanetarium.weebly.com/. Retrieved February 10, 2014. "Lynx Exhibits". Retrieved ...
January 27 - Susan Aglukark, singer-songwriter January 29 - Sean Burke, ice hockey player February 26 - Gene Principe, sports ... reporter March 16 - Kevin Draxinger, swimmer April 5 - Gary Gait, lacrosse player April 5 - Paul Gait, lacrosse player and ...
Greg Gumbel, sportscaster for NFL on CBS Nia-Malika Henderson, CNN political reporter Lester Holt, journalist and anchor for ... Jazz Gene McDaniels: Jazz, pop Donnie McClurkin: Gospel Betty McGlown: R&B, pop Joe McPhee: Jazz Remy Ma: Hip hop Lee Michelle ... "18, Activist, Harvard-Bound: How Yara Shahidi Became the Face and Brain of Young Hollywood". The Hollywood Reporter. Retrieved ... actor Gene Anthony Ray, actor Lance Reddick, actor Della Reese, actress Rakim, rapper Daphne Maxwell Reid, actress and comedian ...
"Wigan Reporter 'Music Scene' Article". Retrieved 3 May 2001. "Album Releases". "NME News Article". Retrieved 28 July 2001. " ... The Seahorses and Gene, before going on to self-release their debut single, "Quarantine", through Valiant Recordings on 23 ...
Park, Gene. "Here are the nominees for The Game Awards 2020". Washington Post. ISSN 0190-8286. Retrieved November 18, 2020. ... Star Wars Jedi: Fallen Order' Director Explains 'Zelda' Influences, Game's Difficulty". The Hollywood Reporter. June 8, 2019. ...
Show hosts: Dink Bernardi Larry Bledsoe Jack Bowen Robert D. "Bob" Coker Carole Arnold Jerry Bohnen Gene Collett Carlton ... formerly a reporter at CBS affiliate KWTV-TV) Mike "Road King" Rogers Scott Rowland Cynthia Rozmaryn David Rucker Mary Shea ... Jim Rupe Al Eshbach News reporters and anchors: Tim Allen Phil Bacharach Bill Bateman Natalie Bell Megan Bishop Jerry Bohnen ... Ken Johnson Craig Logsdon Beth Meyers Dan Mahoney Karen McCoy Gene Molter Bill Mondora Reid Mullins Mark Myers Derrick Nance ...
CS1 maint: discouraged parameter (link) Freese, Gene Scott (2014). Hollywood Stunt Performers, 1910s-1970s: A Biographical ... in which Steele can be glimpsed as an aggressive reporter jumping onto the running board of Kane's car as it pulls away. In the ...
"Emmys: Netflix Beats HBO With Most Nominations". The Hollywood Reporter. July 12, 2018. Retrieved July 12, 2018. "Twin Peaks". ... Mover Joe Adler as Roger Tammie Baird as Lorraine Jim Belushi as Bradley Mitchum John Billingsley as Doctor Ben Ronnie Gene ... Fienberg, Daniel (May 21, 2017). "'Twin Peaks': TV Review". The Hollywood Reporter. Retrieved May 22, 2017. Stephens, Emily L ... Abramovitch, Seth (April 20, 2020). "How I'm Living Now: David Lynch, Director". The Hollywood Reporter. Retrieved May 25, 2020 ...
There, he candidly discussed his thoughts about the political climate, unaware that reporters were also in the room and that ... Oberdorfer, p. 20 Oberdorfer, p. 174 Pearson, Drew (March 12, 1968), "Gene McCarthy Gets Praise for Views on McCarthyism", ... "Gene Vows He Won't Back HHH", St. Petersburg Times, St. Petersburg, Florida, p. 1, August 30, 1968 Macartney, Roy (August 31, ...
Gene Nora Jessen (2002), "Ocean Flying", Powder Puff Derby Of 1929, Sourcebooks, p. 269, ISBN 9781402229725 Douglas 2015, p. ... reporter and pilot Lotfia Elnadi (1907-2002), first Egyptian woman to earn her pilot license 1933 Ruth Elder, pilot and actress ...
But whenever the mild-mannered reporter would change into his true identity of Superman, Collyer's voice would deepen ... Gene Gropper Film Editor: Joseph Simon Sound Supervisor: Jim Bullock Music Composed and Conducted by John Gart Music Supervised ...
Video "Paul Taylor: Creative Domain: Film Review". The Hollywood Reporter. 12 September 2015. Retrieved 29 December 2015. ... Gene Moore, John Rawlings, William Ivey Long, Jennifer Tipton, Santo Loquasto, James F. Ingalls, Donald York and Matthew ...
Gene expression is imperfectly controlled, and it is possible that random fluctuations in the expression levels of many genes ... Vaidya A, Mao Z, Tian X, Spencer B, Seluanov A, Gorbunova V (July 2014). "Knock-in reporter mice demonstrate that DNA repair by ... A single gene may affect multiple traits. Some traits that increase fitness early in life may also have negative effects later ... In about 85% of tumors, this evasion of cellular senescence is the result of up-activation of their telomerase genes. In most ...
Mace - Hotel Desk Clerk Pat Gleason as Banning - Bond Robber Gene O'Donnell as Bond Robber Donald Kerr as Reporter Howard M. ...
... a top-notch reporter played by Albert Brooks; and a charismatic but less seasoned reporter who later becomes a news anchor, ... After The Mary Tyler Moore Show ended, the show's creators, James L. Brooks and Allan Burns, along with Gene Reynolds, wrote a ... directed by William Wellman and written by former Chicago newspaper reporter Ben Hecht, is about New York newspaper reporter ... As a reporter working for Joseph Pulitzer in 1887, she went undercover by committing herself to Blackwell's Island Asylum, an ...
JAMIE ERDAHL NAMED LEAD REPORTER FOR THE SEC ON CBS "Brad Nessler has intriguing Jake Fromm theory, picks his No. 1". New York ... The duo are joined weekly by Jamie Erdahl, and former NFL referee Gene Steratore. He also provides play-by-play for college ... and sideline reporter Heather Cox. He also covered Saturday afternoon games for ESPN during the regular college basketball ... it was also announced that he would be teamed with former Penn State quarterback Todd Blackledge and sideline reporter Erin ...
In the first film Meyer was played by Theodore Bikel, and in the second by Gene Evans. In 1967, author MacDonald refused ... "Fox Scraps Christian Bale's 'Deep Blue Goodbye' Due to Knee Injury (Exclusive)". The Hollywood Reporter. Retrieved 2020-08-05. ...
Gene Porter (Stephen Collins), (season 2), one of the civic leaders of the town of Willoughby, the town doctor and the father ... Stanhope, Kate (April 15, 2015). "'Revolution' to Be Resurrected Online". Hollywood Reporter. Retrieved April 15, 2015. Chapter ... Martin Shaw (Waleed Zuaiter), the member of the Patriots, who recruited Gene Porter to become the member of the Patriots. He ... The Hollywood Reporter. Retrieved June 30, 2012. "Freestyle/Hard Rock Amusement Park on NBC's Revolution". The Coaster Critic. ...
Hoover declined to use a spokesman, instead asking reporters to directly quote him and giving them handouts with his statements ... Herbert Hoover: A Life (2016), 464pp; comprehensive scholarly biography Smith, Gene. The Shattered Dream: Herbert Hoover and ... However, he changed his press policies after the 1929 stock market crash, screening reporters and greatly reducing his ...
The club never acknowledged his absence, simply declining to answer questions about his progress until reporters noticed ... as the replacement of guard Greg Horton with converted defensive lineman Gene Sanders and his greater speed was expected to ...
Caldwell and Gene S. Graham, Nashville Tennessean, "for their exclusive disclosure and six years of detailed reporting, under ... its fact-checking initiative during the 2008 presidential campaign that used probing reporters and the power of the World Wide ...
Some of the most common assays are: ELISAs Protein and cell growth assays Protein:protein interactions Reporter assays Nucleic ... based gene expression assays, as well as cell viability, cytotoxicity, and biorhythm assays based on the luminescent detection ... allows for detection of assays that contain multiple luminescent reporter enzymes, the development of new luminescence assays, ...
Reporter (uncredited) The Girl Next Door (1953) - Reporter (uncredited) The Kid from Left Field (1953) - Fan (uncredited) ... The Gene Krupa Story (1959) - Kenny Lemay (uncredited) Ma Barker's Killer Brood (1960) - Sheriff #1 Voyage to the Bottom of the ... Reporter (uncredited) My Lucky Star (1938) - Saier Five of a Kind (1938) - Reporter (uncredited) Submarine Patrol (1938) - ... Reporter (uncredited) The Grapes of Wrath (1940) - Joe Johnny Apollo (1940) - Reporter (uncredited) Star Dust (1940) - Desk ...
Siskel, Gene (June 21, 1973). "Here's an ungodly skin flick that's a cut above". Chicago Tribune. Section 2, p. 5. Canby, ... McNeil, p. 268 "How a Nasty Legal Fight Over 'Deep Throat', 'Debbie Does Dallas' Was Settled". The Hollywood Reporter. ... Alone among porno stars, she never seems exploited." Gene Siskel of the Chicago Tribune also gave the film three stars out of ...
1969) La bohème (Giacomo Puccini): Fausto Cleva conducting the Metropolitan Opera Orchestra, with Richard Tucker and Gene ... but the Time reporter attributed the latter comment to Callas. According to John Ardoin, however, these two singers should ...
... sideline reporters) Coming off their Wild Card road win over the Vikings, the Eagles took a bus up the NJ Turnpike to Giants ... Gene Steratore TV announcers (Fox): Joe Buck, Troy Aikman, & Pam Oliver The Eagles dropped a divisional matchup to a Washington ... Gene Steratore TV announcers (Fox): Kenny Albert, Daryl Johnston, & Tony Siragusa After the win over Arizona on Thanksgiving, ...
Gene R. Garthwaite (2008). The Persians. Wiley. ISBN 978-1-4051-4400-1. Sigfried J. de Laet. History of Humanity: From the ... "Iranian court imposes total ban on Telegram". Reporters Without Borders. 4 May 2018. Retrieved 2 December 2018. "Iran's ...
Henry Winkler as Gene Cousineau, an acting coach and Barry's mentor. Cousineau serves as a father figure to Barry, helping him ... Malkin, Marc (March 22, 2018). "Bill Hader on Why He Was "Very Nervous" to Play a Hitman in 'Barry'". The Hollywood Reporter. ... and More Reflect on a Year in Lockdown". The Hollywood Reporter. March 18, 2021. Retrieved April 15, 2021. Schwartz, Dana ( ... Kilday, Gregg (January 8, 2019). "Dorian Awards: 'The Favourite' Named Film of the Year". The Hollywood Reporter. Archived from ...
Railway News Reporter, Dec. 1890, quoted in "L.W. Rogers". Railroad Trainmen's Journal. Vol. 8 no. 12. December 1892. pp. 878- ... an organization springing in large part from activists loyal to Gene Debs and his ARU, Rogers became involved in the affairs of ... the government returned to court charging that ARU head Gene Debs, Vice President George W. Howard, Secretary Sylvester Keliher ...
Reporter genes. Definition. Reporter genes are genes that enable the detection or measurement of gene expression. They can be ... Reporter genes include genes that code for fluorescent protein and enzymes that convert invisible substrates to luminescent or ... Role of Proinflammatory Cytokines in Feedback Modulation of Circadian Clock Gene Rhythms by Saturated Fatty Acids *Sam-Moon Kim ... A tetracycline-dependent ribozyme switch allows conditional induction of gene expression in Caenorhabditis elegans Tools for ...
... corniche information info at corniche.com Tue Apr 21 23:06:07 EST 1998 *Previous message: Test ... Do you know any nuclear reporter gene I can use? Thank you very much! Zhe Han zhan at umich.edu ... I am using beta-gal to show the expression pattern of this gene. But the result is not so good because beta-gal is expressed in ... I am studying cis-regulation of a gene encoding nuclear protein. ...
... Cornelius Krasel krasel at alf.biochem.mpg.de Tue Jan 11 06:58:10 EST 1994 *Previous message: Reporter ... The wild type SV40-t-intron is the generic donor for this function : and is widely used in a variety of reporter gene vectors, ... Other features of these vectors include a very : versatile multiple cloning site upstream and downstream of the : reporter gene ... reporter gene vectors. What distinguishes : them from other vectors in this category is that these vectors produce : near-zero ...
Reporter gene definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. Look it up ... a gene with an easily recognizable phenotype, used in analysing the regulation of gene structures ...
Sherman started his 30 years on staff as a cub reporter covering nearly all the regular news beats from police and sheriff to ... He then worked as a rewrite man, a frontline general assignment reporter, leading feature story writer, war correspondent, in- ... depth investigative reporter and a foreign correspondent. He became a daily general interest writer of his page-2 column ... Cityside for seven years and a roving national and international assignment reporter. In 1964 he opened the London bureau as ...
... typically to discern patterns of gene expression, but also as markers of transformed cells during stable transformation ... Reporter genes have been widely used in plant molecular biology, ... GUS Protocols: Using the GUS Gene as a Reporter of Gene ... detection of expression of the gus reporter gene transgenic plants: ten years of blue genes Transgenic Res. 5,281-288.CrossRef ... Reporter genes have been widely used in plant molecular biology, typically to discern patterns of gene expression, but also as ...
Stable cell lines containing HIV Tat-regulated reporter genes are now available that permit rapid, sensitive and reproducible ... Measuring HIV neutralization in a luciferase reporter gene assay.. Montefiori DC1. ... reporter gene assay performed in TZM-bl (JC53bl-13) cells was recently optimized and many of its performance parameters have ...
To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA ... a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest ... In these cases, trans-acting elements, such as transcription factors are used to express the reporter gene. Reporter gene assay ... the reporter genes expression is independent of the gene of interests expression, which is an advantage when the gene of ...
Gene Cernan, Last Astronaut on Moon, Dies at 82 1:39 PM PST 1/16/2017 by the Associated Press ... Former astronaut Gene Cernan, the last of only a dozen men to walk on the moon and who returned to Earth with a message of ... Even at the age of 82, Gene was passionate about sharing his desire to see the continued human exploration of space and ... We are saddened by the loss of retired NASA astronaut Gene Cernan, the last man to walk on the moon. https://t.co/Q9OSdRewI5 ...
Thus, the GUS gene is the reporter gene of choice for transgenic plant research. Not only can this assay be used to detect ... researchers to effectively use the GUS gene as a reporter of the expression of introduced genes.First book on the GUS reporter ... is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes ... whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. Low cost is ...
Full gene cassettes including the 5UTR from the M gene, the reporter gene and the 3 UTR of the P gene were inserted for ... A full-length antigenome clone of HeV was assembled, a reporter gene (GFP or luciferase) inserted between the P and M genes and ... and viruses expressing two different reporter genes encoded as an additional gene cassette inserted between the P and M genes. ... Recombinant Hendra viruses expressing a reporter gene retain pathogenicity in ferrets.. Marsh GA1, Virtue ER, Smith I, Todd S, ...
... reporter genes is an important application for cell-based tracking, drug screening, and therapy. The firefly luciferase ,i,(fl ... were fused together to create TF reporter gene constructs with different order. The enzymatic activities of TF protein in vitro ... We have developed an optimized triple fusion reporter construct ,i,DsRedm-fl-ttksr39,/i, for more effective and sensitive ,i,in ... imaging but may expedite FACS-based selection of TF reporter expressing cells. ...
Such reporters are used in methods for monitoring cultures for production of target compounds. ... Compositions and methods are provided for enhanced expression of light emitting reporters. ... supra) and the lacZ reporter cassette (Tummala, S. B. et al. Development and characterization of a gene expression reporter ... a) Gene ID: Systematic gene code from TIGR; b) Direction: Coding strand; c) Annotation: Gene symbol according to TIGR; d) IR ...
... and kits for expressing and detecting a variety of reporter genes including luciferase, glycosidases, Chloramphenicol ... In these assays, the reporter gene acts as a surrogate for the coding region of the gene under study. The reporter gene ... Reporter gene assays offering chemiluminescent measurement of reporter gene activity that is more sensitive than colorimetric ... Reporter gene assays are invaluable for studying the regulation of gene expression by cis- or trans-acting factors, or for ...
Reporter genes are genes whose products can be readily assayed subsequent to transfection and used as markers for screening ... The reporter gene construct contains one or more gene regulatory elements to be analyzed, the sequence for the reporter gene, ... reporter genes used for screening transfectants make the cells containing the reporter gene visually identifiable. Reporter ... Gene regulation assays Reporter gene assays are invaluable for studying regulation of gene expression, both by cis-acting ...
We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of ... Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water ... Artificial reporter gene providing MRI contrast based on proton exchange Nat Biotechnol. 2007 Feb;25(2):217-9. doi: 10.1038/ ... Existing magnetic resonance reporter genes all rely on the presence of (super)paramagnetic substances and employ water ...
Renilla and Nanoluc luciferase reporters. This guide discusses using bioluminescent reporter genes to study eukaryotic gene ... expression, regulation and cellular events and measuring activity with single-reporter assays, dual-reporter assays, and live- ... An introduction to reporter genes with a focus on firefly, ... Reporter Gene Vectors Measuring Reporter Gene Activity Reporter ... Introduction to Reporter Genes. What is a Reporter Gene?. A reporter gene is an exogenous coding region joined to a promoter ...
Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. E Yeh, K Gustafson, and G L ... Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila ... Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila ... Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila ...
britelite plus Reporter Gene Assay System 6066761 , 6066766 , 6066769 Our brightest reporter gene assay for quantitation of ... steadylite plus Reporter Gene Assay System 6066756 , 6066751 , 6066759 Reporter gene assay with longest-lasting signal for ... neolite Reporter Gene Assay System 6016719 , 6016711 , 6016716 Glow luminescence reporter gene assay combining benefits of both ... twinlite Firefly and Renilla Luciferase Reporter Gene Assay System 6066709 , 6066706 twinlite is a dual luciferase reporter ...
o so a reporter gene is just a tag...it works with any gene? ... Re: Reporter Genes. * Quote Post by id10t » Sat Mar 12, 2011 8: ... Reporter Genes. Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field ... How do you know what reporter gene to use when setting up a deletion analysis, or any other experiment? ...
... gene expression regulation · gene induction · gene location · mouse · nonhuman · reporter gene · Animals · Cell Line · Cell ... Gene deletion · Gene expression · Mutagenesis · Nonhuman · PagsA gene · Priority journal · Reporter gene · Screening test · ... reporter gene · short survey · signal transduction · Bacteria · Environmental Monitoring · Gene Expression · Genes, Reporter · ... Electrophile responsive element mediated gene · Enzyme induction · Gene · Gene activation · Gene expression · Gene induction · ...
Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were ... Reporter constructs with low background activity utilizing the cat gene Gene. 1992 Jan 2;110(1):129-30. doi: 10.1016/0378-1119( ... Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were ...
... siSTABLE controls are specially modified for ... Choose from siSTABLE-modified controls, siRNA for silencing reporter genes (GFP, Luciferase, etc.) or other experimental needs. ... Control siRNAs for commonly used reporter systems (GFP, Luciferase, etc.) have been experimentally validated for functionality ...
REPORTER GENE ASSAY I have a question regarding the luciferase reporter gene assay. I performed transient transfection of MCF-7 ... cells to 100nM TCDD and found the increase in activity of CYP1B1 promotor activity when i performed luciferase reporter gene ... cells using CYP1B1 reporter plasmid and exposed the ... REPORTER GENE ASSAY. 1 post / 0 new Log in or register to post ... Home/ Forums/ General: Student Questions/ REPORTER GENE ASSAY. ...
Home/ Forums/ Cloning (Recombinant Gene Expression): Reporter Assays/ REPORTER GENE ASSAY. REPORTER GENE ASSAY. 3 posts / 0 new ... REPORTER GENE ASSAY I have a question regarding the luciferase reporter gene assay. I performed transient transfection of MCF-7 ... cells to 100nM TCDD and found the increase in activity of CYP1B1 promotor activity when i performed luciferase reporter gene ... cells using CYP1B1 reporter plasmid and exposed the ...
HealthDay Reporter ...THURSDAY Dec. 16 (HealthDay News) -- U.S. scientists have unraveled t...Gene sequencing reveals that this ... Gene,Research,Brings,Insight,Into,Deadly,Childhood,Brain,Tumor,medicine,medical news today,latest medical news,medical ... Gene Research Brings Insight Into Deadly Childhood Brain Tumor. By Alan Mozes HealthDay Reporter ...THURSDAY Dec. 16 (HealthDay ... HealthDay Reporter THURSDAY, Dec. 16 (HealthDay News) -- U.S. scientists have unraveled the genetic code for the most common ...
Get Hawaii Reporter Email Updates Enter your email address to subscribe and receive notifications of new posts by email. ... The point is that legislators should not be in the business of passing laws that endanger the health of the human gene pool. If ... Home News CHARLEYWORLD: Laws Protecting the Stupid Are Bad For The Gene Pool ... CHARLEYWORLD: Laws Protecting the Stupid Are Bad For The Gene Pool. By ...
It has been reported that ferritin gene, an emerging MRI reporter gene, can enhance the contrast and increase the sensitivity ... Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation. Yupeng Feng,1,2 Qicai Liu,3 ... M. Campan, V. Lionetti, G. D. Aquaro et al., "Ferritin as a reporter gene for in vivo tracking of stem cells by 1.5-T cardiac ... K. Ziv, G. Meir, A. Harmelin, E. Shimoni, E. Klein, and M. Neeman, "Ferritin as a reporter gene for MRI: chronic liver over ...
Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1.. C F Lesser and ... Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1.. C F Lesser and ... Mutational analysis of pre-mRNA splicing in Saccharomyces cerevisiae using a sensitive new reporter gene, CUP1.. C F Lesser and ... We have developed a new reporter gene fusion to monitor mRNA splicing in yeast. An intron-containing fragment from the ...
... used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to ... Reporter gene expression analysis. His+ transformants (a mixture of 10 colonies randomly chosen) were recovered from a SDsup ... 1a) as segregational stability was expected to drop, both due to an increased size and reporter gene expression (Parker and ... 2017). A family of plasmids varying in their architecture was assembled by addition of a yEGFP3-based reporter gene in ...
  • Reporter gene assays are invaluable for studying the regulation of gene expression by cis- or trans-acting factors, or for measuring activation of cell signaling pathways. (thermofisher.com)
  • In these assays, the reporter gene acts as a surrogate for the coding region of the gene under study. (thermofisher.com)
  • Reporter gene assays are typically used to measure the regulatory ability of an unknown DNA-sequence. (thermofisher.com)
  • Reporter gene assays are invaluable for studying regulation of gene expression, both by cis -acting factors (gene regulatory elements) and trans -acting factors (transcription factors or exogenous regulators). (thermofisher.com)
  • Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. (perkinelmer.com)
  • With the advent of dual-reporter assays, like Promega's DLR™ assay, reporter genes became more accurate, more linear and better at accounting for expression activity and cell proliferation unrelated to the promoter of interest. (bmglabtech.com)
  • The Galacto-Light™ beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of b-galactosidase in reporter gene assays. (selectscience.net)
  • The major factors driving the growth of this market include increasing funding for cell-based research, growing applications of gene expression, and a rising preference for cell-based assays. (express-press-release.net)
  • The assay kits segment is expected to account for the larger share of the reporter gene assays market in 2019. (express-press-release.net)
  • I learnt that reporters can be adopted to study cell microchimerism , cell fusion , membrane biogenesis , while different assays were aimed at understanding protein interaction . (reportergene.com)
  • Reporter assays were exploited not only to address basic biology questions, but also to help solving related problems, like heavy metal detection , DNA sequencing , or to faithfully report conditional transgenesis . (reportergene.com)
  • The accuracy of reporter assays can be improved by utilizing a dual reporter system. (creativebiomart.net)
  • Progress in methodology improved reporter gene assays used to identify ligands acting on orphan seven-transmembrane receptors. (lu.se)
  • Until now, reporter gene assays have not been in common use in this process. (lu.se)
  • Here, we summarize our development of improved reporter gene assays. (lu.se)
  • We optimized reporter gene assays in respect of (i) the promoter region of the construct, (ii) the. (lu.se)
  • To evaluate results from the PXR reporter gene assay with those from the aforementioned conventional assays, 14 drugs were evaluated for their ability to induce CYP3A4 and activate PXR. (aspetjournals.org)
  • Although this approach is useful in many cases, the limited sensitivity of reporter assays can prevent studies in cases where few cells are obtainable or efficiency of transfection is low. (eurekamag.com)
  • Reporter gene assays provide a simple, high-throughput methodology for examining the transcriptional activation of CYP gene expression by xenobiotics. (surrey.ac.uk)
  • CAT & Luciferase (Luc) reporter gene vectors. (bio.net)
  • and is widely used in a variety of reporter gene vectors, eg. (bio.net)
  • We offer antibodies, reagents, vectors, and kits for expressing and detecting a variety of reporter genes including luciferase, glycosidases, chloramphenicol acetyltransferase (CAT), green fluorescent protein (GFP), β-lactamase, and β-gal, as well as Lumio™ and TC-FlAsH™ technology. (thermofisher.com)
  • Dear justwonder, The synthetic poly(A) signal in the pCAT3 vector and several other Promega vectors is placed upstream of the reporter to eliminate background and vector dependent reporter signal. (protocol-online.org)
  • Basically, all vectors have cryptic transcription factor binding sites that will cause the reporter to be transcribed. (protocol-online.org)
  • The phCMV Fusion Stable Reporter (FSR) Vectors are designed for high-level expression of GFP or Luciferase fusion proteins and for creating GFP or Luciferase stable cell lines. (genlantis.com)
  • The vectors can be used for either transient transfection or for generating stable cells lines expressing the reporter gene only or your fusion-reporter gene construct. (genlantis.com)
  • The reporter gene is constitutively expressed, and reporter imaging is used to track gene therapy vectors or transduced cells in the body ( 8 ). (snmjournals.org)
  • Here, we characterise lentivirus-based bi-cistronic reporter gene vectors and silica-coated gold nanorods (GNRs) as synergistic tools for cell labelling and tracking. (elifesciences.org)
  • Addgene: A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in Gram-negative bacteria. (addgene.org)
  • These expression vectors contain a specific gene of interest as well as a GFP reporter gene. (cellbiolabs.com)
  • Presently circulating nematode transcriptional gene expression vectors use the viral NLS from simian virus 40 (SV40) large T antigen. (elsevier.com)
  • A dual or triple fusion reporter gene construct harboring various fluorescent proteins (i.e., enhanced green fluorescent protein ( egfp ), red fluorescent protein ( rfp ), and different luciferase genes (i.e., firefly luciferase ( fl ), renilla luciferase ( rl ), and herpes simplex virus type 1 thymidine kinase ( tk ) gene)) can combine the advantage of fluorescence, bioluminescence, and radionuclide imaging techniques and overcome the shortcomings of each imaging modality [ 6 ]. (hindawi.com)
  • We have used the green fluorescent protein (GFP) from the jellyfish Aequorea victoria as a vital marker/reporter in Drosophila melanogaster. (pnas.org)
  • Examples include the gene that encodes jellyfish green fluorescent protein (GFP), which causes cells that express it to glow green under blue light, the enzyme luciferase, which catalyzes a reaction with luciferin to produce light, and the red fluorescent protein from the gene dsRed [fr]. (wikipedia.org)
  • Here, we describe a complete characterization of the strategy based on TGL triple-fusion reporter gene--which codifies for Herpes Simplex virus type 1 thymidine-kinase (HSV1-TK), green fluorescent protein (GFP) and Firefly Luciferase--(SFG(NES)TGL) to inactivate encapsulated cells and their therapeutic effects. (sigmaaldrich.com)
  • For fluorescent reporters, choose between enhanced green fluorescent protein (EGFP) and mCherry. (trilinkbiotech.com)
  • Then co-transfected with CXCL12 3'UTR luciferase reporter plasmid and miR-5100 mimics or negative control into HEK293T cells, analysis the luciferase activity 48 hours after transfection, the transfection efficiency was confirmed by transfection of green fluorescent protein GFP. (vde-verlag.de)
  • In the current study lentiviral (LV) vector-mediated gene transfer of a green fluorescent protein (GFP) reporter gene was used to study the feasibility of gene therapy in the reimplanted root to further promote regeneration of motor axons. (unboundmedicine.com)
  • A stable macrophage cell line coexpressing hNIS and green fluorescent protein (GFP) genes (RAW264.7/hNIS-GFP and R NIS cell) was established from an immortalized macrophage cell line (RAW264.7 cells). (snmjournals.org)
  • To investigate the physiological function of CX 3 CR1-FKN interactions, we generated a mouse strain in which the CX 3 CR1 gene was replaced by a green fluorescent protein (GFP) reporter gene. (asm.org)
  • Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay. (oregonstate.edu)
  • This construct contained enhanced green fluorescent protein (EGFP) gene which was controlled by the beta-globin promoter. (sid.ir)
  • The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is a substantial reporter molecule for monitoring gene expression and protein localization.The fluorescence of GFP requires only UV or blue light, and, therefore, the in vivo observation of GFP expression is easy with no need for complex and costly apparatus. (bmmj.org)
  • We quantified expression of two reporter genes, green fluorescent protein ( GFP ) and the herbicide bialaphos resistance gene ( BAR ), in 2256 transgenic poplar trees derived from 404 primary events, and in 106 in vitro-redifferentiated subevents, over 3 years in the greenhouse and in the field. (oup.com)
  • Targeting a gene expression reporter, usually the green fluorescent protein (GFP), to the nucleus via a translationally fused nuclear localization signal (NLS) greatly facilitates recognition and identification of the reporter-expressing cells in Caenorhabditis elegans. (elsevier.com)
  • Upon introduction of the reporter construct into cells, expression levels of the reporter gene are monitored through a direct assay of the reporter proteins enzymatic activity. (thermofisher.com)
  • Commonly used reporter genes that induce visually identifiable characteristics usually involve fluorescent and luminescent proteins. (thermofisher.com)
  • We designed a nonmetallic, biodegradable, lysine rich-protein (LRP) reporter, the prototype of a potential family of genetically engineered reporters expressing artificial proteins with frequency-selective contrast. (nih.gov)
  • As part of a process called gene regulation, these chromatin proteins determine if information stored in a certain portion of the DNA is accessible - the genes are "turned on" - or inaccessible - the genes are "turned off. (uab.edu)
  • Her research addresses how HP1 proteins work together to contribute to gene regulation. (uab.edu)
  • My lab's research centers on a group of conserved proteins, the HP1 family, that contribute to the regulation of gene expression and DNA packaging. (uab.edu)
  • Our approach is to monitor all three HP1 proteins, and to manipulate not only single genes, but also combinations of genes. (uab.edu)
  • Each phCMV-FSR Vector contains a multiple cloning site on either side of the reporter gene for creating N- or C-terminal fusion proteins. (genlantis.com)
  • These proteins include the product of the previously cloned ompH gene (D. H. Bartlett, M. Wright, A. A. Yayanos, and M. Silverman. (asm.org)
  • and rising focus of stakeholders on research projects involving proteins, associated biomolecules, and genes. (express-press-release.net)
  • Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. (plantcell.org)
  • Cotransfection experiments with natural and synthetic AuxRE reporter genes and effector genes encoding Aux/IAA proteins showed that overexpression of Aux/IAA proteins in carrot protoplasts resulted in specific repression of TGTCTC AuxRE reporter gene expression. (plantcell.org)
  • Activation by HHV-6 was due to one or more viral proteins produced early in infection and, in the case of the HTLV-I LTR, was synergistic to induction mediated by the HTLV-I tax gene product. (microbiologyresearch.org)
  • Many of the disease-associated variants are located in "non-coding" regions of the genome - they are not located in genes that code for proteins. (vumc.org)
  • Background: Chlamydomonas reinhardtii is a novel recombinant eukaryotic expression system with many advantages including fast growth rate, rapid scalability, absence of human pathogens and the ability to fold and assemble complex proteins accurately, however, obstacle relatively low expression level necessitates optimizing foreign gene expression in this system. (bmmj.org)
  • Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. (addgene.org)
  • twinlite is a dual luciferase reporter gene assay system designed to detect and quantitate both Firefly and Renilla luciferase sequentially from cultured cells. (perkinelmer.com)
  • twinlite ™ is a dispense-and-read ultra-high sensitivity dual luciferase reporter gene assay system for the quantitation of Firefly and Renilla luciferase expression in mammalian cells. (perkinelmer.com)
  • Reporters that emit at different wavelengths, like Gaussia and Renilla , can be detected at the same time by detecting the different wavelengths using filters. (bmglabtech.com)
  • In the case of green- Renilla , from 450- to 650nm - BMG LABTECH designs filters specifically for dual glow reporters that let light in broadly over the entire peak, allowing as much as 20x more light to be detected. (bmglabtech.com)
  • We have recently demonstrated that Renilla luciferase (Rluc) is a promising bioluminescence reporter gene that can be used for noninvasive optical imaging of reporter gene expression in living mice, with the aid of a cooled charged couple device (CCD) camera. (spiedigitallibrary.org)
  • In the current study, we explore the expression of a novel synthetic Renilla luciferase reporter gene (hRluc) in living mice, which has previously been reported to be a more sensitive reporter than native Rluc in mammalian cells. (spiedigitallibrary.org)
  • S. Bhaumik, X. Z. Lewis, Sanjiv Gambhir, "Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice," Journal of Biomedical Optics 9(3), (1 May 2004). (spiedigitallibrary.org)
  • For bioluminescent imaging, we offer reporter genes luciferase, firefly luciferase (FLuc) and Renilla luciferase mRNAs. (trilinkbiotech.com)
  • Dual Luciferase Reporter Gene Assay Kit is used for sequential measuring the activity of two different luciferases, firefly (FFL) and Renilla (RL), in a single sample. (creativebiomart.net)
  • Here, we show ASP-RNAi against the Swedish- and London-type amyloid precursor protein (APP) variants related to familial Alzheimer's disease using two reporter alleles encoding the Photinus and Renilla luciferase genes and carrying mutant and wild-type allelic sequences in their 3'- untranslated regions. (alliedacademies.org)
  • In this article, we describe an easy assay system for assessment of ASP-RNAi with mutant and wild-type reporter alleles encoding the Photinus and Renilla luciferase genes. (alliedacademies.org)
  • Require Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require injection of substrate to produce bioluminescence. (addgene.org)
  • Reporter genes include genes that code for fluorescent protein and enzymes that convert invisible substrates to luminescent or coloured products. (nature.com)
  • Hi, I am studying cis-regulation of a gene encoding nuclear protein. (bio.net)
  • The firefly luciferase (fl) , monomeric red fluorescence protein (mrfp) , and truncated herpes simplex virus type 1 thymidine kinase SR39 mutant (ttksr39) were fused together to create TF reporter gene constructs with different order. (hindawi.com)
  • Following introduction of the reporter construct into cells and experimental treatment, expression levels of the reporter gene are monitored by quantitating the reporter protein enzymatic activity, which is used to characterize regulatory sequences, transcription factor activity, cell signaling, or signaling pathways. (thermofisher.com)
  • Common reporter genes include beta-galactosidase, luciferase, beta-lactamase, alkaline phosphatase and GFP (green fluorescence protein). (thermofisher.com)
  • Luminescence, absorbance and fluorescence detection methods are used typically to measure expressed reporter gene protein. (thermofisher.com)
  • A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. (wikipedia.org)
  • Reporter genes can produce a protein that has little obvious or immediate effect on the cell culture or organism. (wikipedia.org)
  • Reporter gene assay have been increasingly used in high throughput screening (HTS) to identify small molecule inhibitors and activators of protein targets and pathways for drug discovery and chemical biology. (wikipedia.org)
  • Often, to simplify interpretation of results, only one gene or protein is manipulated. (uab.edu)
  • This implies that SCL is critical for the expression of genes needed to initiate hematopoiesis, that a multiprotein complex is required (hence the need for the bridging protein LMO2 ), but that the other known components of this complex are at least partly redundant at this stage. (bloodjournal.org)
  • Based on application, the reporter gene assay market is segmented into gene regulation, protein interaction, cell signaling pathways, and promotor structural & functional analysis. (express-press-release.net)
  • The revertant may have either a defective target protein whose activity is essential for the transforming activity of ras or an activated tumor suppressor gene which can suppress the activity of ras . (aacrjournals.org)
  • 125 I uptake was determined (for hNIS protein functional activity), and flow cytometry analysis (to examine GFP gene expression), a cell proliferation assay, a cytokine assay, and a phagocytic activity assay were performed. (snmjournals.org)
  • Co-expression of the bioluminescence reporter luciferase and the optoacoustic reporter near-infrared fluorescent protein iRFP720 enabled cell tracking over time in mice. (elifesciences.org)
  • Hiramatsu N, Kasai A, Hayakawa K, Nagai K, Kubota T, Yao J, Kitamura M. (2006) Secreted protein-based reporter systems for monitoring inflammatory events: critical interference by endoplasmic reticulum stress. (celltechnology.com)
  • These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. (eurekamag.com)
  • We added poly(A) tails to the 3' ends of siRNA antisense strands by in vitro transcription, and investigated the silencing effects of poly (A)- tailed siRNAs on enhanced green fluorescence protein (EGFP) and red fluorescence protein (RFP) genes. (alliedacademies.org)
  • They are widely used in application like cell signaling pathways, gene regulation, structural and functional analysis and protein interaction which is the major factor fueling the growth of this market. (databridgemarketresearch.com)
  • Another source of consistent, but apparently ectopic expression is an unavoidable drawback of reporter genes, and is a function of the reporter gene products (mRNA and protein) and their stability within the cellular environment (see Note 37). (alpfmedical.info)
  • The promoter region is the specific DNA sequence that regulates the transcription of the gene and can activate or repress the expression of the gene. (thermofisher.com)
  • In these cases, trans-acting elements, such as transcription factors are used to express the reporter gene. (wikipedia.org)
  • The large share of the assay kits segment can be attributed to their repeated use in gene expression studies and signaling pathway analysis during gene transcription or translation levels. (express-press-release.net)
  • We have used a non-replicating recombinant adenovirus, Ad5MCMVlacZ, which expresses the β-galactosidase reporter gene, to examine both constitutive and inducible repair of UV-damaged DNA in repair proficient CHO-AA8 Chinese hamster ovary cells and in mutant CHO-UV61 cells which are deficient in the transcription-coupled repair (TCR) pathway of nucleotide excision repair. (portlandpress.com)
  • Oncogenic activation of ras results in changes in the transcription of several genes leading to uncontrolled cell growth. (aacrjournals.org)
  • Although it is clear that a reporter gene can be used to study gene expression, and in 2008 new advances were from the profiling of multiple transcription factors to the ability to characterize intron-delayed transcriptional clocks , a genetically-encoded assay can be designed to study more than gene expression. (reportergene.com)
  • Gene Regulation: gene expression level, characterization of promoter activity, modulation of gene expression by receptors, transcription factors and small molecules.Drug Discovery: high-throughput screen for gene modulators. (creativebiomart.net)
  • As a means of investigating gene function, we developed a robust transcription fusion reporter vector to measure gene expression in bacteria. (asm.org)
  • Results In order to test the potential effect of G4s on retrotransposon expression, we cloned the long terminal repeats of selected maize LTR retrotransposons upstream of the lacZ reporter gene and measured its transcription and translation in yeast. (muni.cz)
  • The cell line was further demonstrated to be a sensitive reporter of the endogenous MMP12 promoter by applying transcription factors STAT3, AP-1 and SP-1 to the cell line. (ejbiotechnology.info)
  • The DR5 AuxRE showed greater auxin responsiveness than a natural composite AuxRE and the GH3 promoter when assayed by transient expression in carrot protoplasts or in stably transformed Arabidopsis seedlings, and it provides a useful reporter gene for studying auxin-responsive transcription in wild-type plants and mutants. (plantcell.org)
  • Data from these studies indicate that induction of ER-reporter activity alone does not necessarily parallel endogenous gene transcription, and that the reporter gene assay may detect interactions that are not functional in vivo. (ovid.com)
  • Gene reporter technology (GRT) has opened several new avenues for monitoring biological events including the activation of transcription factors, which are central to the study of nutrigenomics. (oregonstate.edu)
  • Use of reporter genes to measure xenobiotic-mediated activation of CYP gene transcription. (surrey.ac.uk)
  • We modified the common transcriptional reporter SV40 NLS-GFP by adding to its C terminus a cognate putative NLS from the transcription factor egl-13. (elsevier.com)
  • The red fluorescence signal is relatively weak for in vivo imaging but may expedite FACS-based selection of TF reporter expressing cells. (hindawi.com)
  • We have developed an optimized triple fusion reporter construct DsRedm-fl-ttksr39 for more effective and sensitive in vivo animal imaging using fluorescence, bioluminescence, and PET imaging modalities, which may facilitate different fields of biomedical research and applications. (hindawi.com)
  • Reporter genes have been used for several decades to study regulation of gene expression in vivo. (emka.si)
  • TriLink offers a range of convenient reporter mRNAs for your cell culture and in vivo experiments. (trilinkbiotech.com)
  • Bioluminescent reporters are useful for time-course studies in cells and in vivo . (trilinkbiotech.com)
  • Fluorescence gene reporters have recently become available for excitation at far-red wavelengths, enabling opportunities for small animal in vivo gene reporter fluorescence tomography (GRFT). (spiedigitallibrary.org)
  • In Vivo magnetic resonance imaging of xenografted tumors using FTH1 reporter gene expression controlled by a tet-on switch. (genscript.com)
  • The controllable imaging system we have developed minimizes risks from constitutive reporter gene overexpression and facilitates tumor cell monitoring in vitro and in vivo. (genscript.com)
  • Furthermore, our results demonstrate that weakly-activated PKR and reduced stability of mRNAs of exogenous reporter genes in vivo may be the possible mechanisms of this non-specific enhanced silencing effect. (alliedacademies.org)
  • To observe bacteria in action, researchers have developed in vivo bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these reporters has its drawbacks. (addgene.org)
  • In vivo imaging with fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint what tissue or organ this signal is coming from. (addgene.org)
  • The human ChM-1 gene was assigned to chromosome 13q14-21 by fluorescence in situ hybridization (FISH) using the clone as a probe. (sigmaaldrich.com)
  • We employed multiple projections of the far-red fluorescence gene reporters IFP1.4 and iRFP, excited by a point source in transillumination geometry in order to reconstruct the location of orthotopically implanted human prostate cancer (PC3), which stably expresses the reporter. (spiedigitallibrary.org)
  • Furthermore, an unique fluorescence-based clone selection step was introduced, allowing rapid selection of the most sensitive reporter cell clones when establishing stable reporter cell lines. (lu.se)
  • The EGL-13 NLS effected clear contrast in fluorescence intensity between the nucleus and the cytoplasm in cells with strong reporter signal and efficiently highlighted the nucleus in tissues with weak reporter expression in a wide range of tested tissues. (elsevier.com)
  • I performed transient transfection of MCF-7 cells using CYP1B1 reporter plasmid and exposed the cells to 100nM TCDD and found the increase in activity of CYP1B1 promotor activity when i performed luciferase reporter gene assay,I repeated the same experimental procedure for seven times and i could't get the same results and plzz suggest what could be the posiible reason for my poor results. (scientistsolutions.com)
  • Multicopy episomal plasmids in yeast, used whenever elevated levels of foreign or homologous gene expression are necessary, are known to be less stable compared to the endogenous 2-μm plasmid they are based on, at least without selective pressure. (springer.com)
  • In this study, we changed the architecture of a multicopy model expression plasmid, creating six isoforms (same size, same DNA content but different positions and orientations of the expression block) and studied mitotic stability, copy number, as well as reporter yEGFP3 expression between isoforms. (springer.com)
  • For the plasmid set studied, yEGFP3 reporter production depends more on mitotic stability than on elevated plasmid copy numbers in a small number of cells retaining the plasmid under non-selective conditions. (springer.com)
  • In both cases, expression levels are, however, generally low due to the presence of only one copy of the plasmid and thus of the gene of interest (Caunt et al. (springer.com)
  • In this study, human CXCL12 3'UTR luciferase reporter plasmid was successfully constructed. (vde-verlag.de)
  • The result showed that transfection of miR-5100 mimics can decrease the luciferase activity of CXCL12 3'UTR luciferase reporter plasmid compared with the negative control group. (vde-verlag.de)
  • A549-NFκB reporter cells stably transfected with a NFκB-luciferase plasmid were exposed to each CSC and NFκB activation was determined by measuring changes in luciferase activity in cell lysates via a standard luciferase assay. (aacrjournals.org)
  • Cells are transfected with a plasmid containing a promoter with a human growth hormone (hGH) receptor gene. (eurekamag.com)
  • these ensure exclusive amplification on the hGH cDNA and not the reporter plasmid. (eurekamag.com)
  • Electroporation enhances reporter gene expression following delivery of naked plasmid DNA to the lung. (cfgenetherapy.org.uk)
  • Our reporter gene assay reagents produce a long-lived glow for plate batch processing and simplified, automated, cost-effective screens. (perkinelmer.com)
  • Reporter genes are genes that enable the detection or measurement of gene expression. (nature.com)
  • detection of expression of the gus reporter gene transgenic plants: ten years of blue genes Transgenic Res. (springer.com)
  • The ideal reporter gene should be absent from the cells used in the study or easily distinguishable from the native form of the gene, assayed conveniently, and have a broad linear detection range. (thermofisher.com)
  • Luminescence detection of reporter genes has been one of the key tools in a molecular biologist's toolbox for decades. (bmglabtech.com)
  • With the ability to detect luminescence emission at 450 and then at 620, the glow from one reporter doesn't interfere with detection of the other. (bmglabtech.com)
  • BMG LABTECH instruments are especially well-suited for the detection of dual glow reporters since they can measure two emission wavelengths simultaneously. (bmglabtech.com)
  • Cell Technology's aCellaTM - SEAP detection kit utilizes a Chemiluminescent-based assay technique to detect SEAP reporter gene expression. (celltechnology.com)
  • Detection of the functional activity of NF-ĸB/SEAP stable reporter cell line. (celltechnology.com)
  • We here describe an assay for the detection of interferon-like activity in Atlantic salmon based on the transient transfection of chinook salmon embryo cells (CHSE-214 cells) with a rainbow trout Mx1 promoter linked to a luciferase reporter. (semanticscholar.org)
  • Reporter genes have been widely used in plant molecular biology, typically to discern patterns of gene expression, but also as markers of transformed cells during stable transformation procedures. (springer.com)
  • Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. (google.com)
  • A full-length antigenome clone of HeV was assembled, a reporter gene (GFP or luciferase) inserted between the P and M genes and transfected to 293T cells to generate infectious reporter gene-encoding recombinant viruses. (nih.gov)
  • Reporter genes are genes whose products can be readily assayed subsequent to transfection, and can be used as markers for screening successfully transfected cells, for studying regulation of gene expression, or serve as controls for standardizing transfection efficiencies. (thermofisher.com)
  • It is also important that the presence of the reporter gene does not affect the normal physiology and general health of the transfected cells. (thermofisher.com)
  • In contrast to selectable markers, which protect an organism from a selective agent that would normally kill it or prevent its growth, reporter genes used for screening transfectants make the cells containing the reporter gene visually identifiable. (thermofisher.com)
  • Reporter genes used in this way are normally expressed under their own promoter independent from that of the introduced gene of interest, allowing the screening of successfully transfected cells even when the gene of interest is only expressed under certain specific conditions or in tissues that are difficult to access. (thermofisher.com)
  • X-gal) is added, cells expressing the gene convert the X-gal to a blue product and can be seen with the naked eye. (thermofisher.com)
  • Our brightest reporter gene assay for quantitation of firefly luciferase in mammalian cells. (perkinelmer.com)
  • Reporter gene assay with longest-lasting signal for quantitation of firefly luciferase in mammalian cells. (perkinelmer.com)
  • Only those cells that have successfully taken up the construct containing the CAT gene will survive and multiply under these conditions. (wikipedia.org)
  • Reporter plasmids utilizing the cat gene for the analysis of promoter and enhancer sequences in vertebrate cells, were constructed. (nih.gov)
  • Unlike superparamagnetic iron oxide particles (SPIOs) and other particle-based techniques [ 13 , 14 ], the genetic modified transgene of FTH1, like other MR reporters, [ 15 , 16 ] would not be diluted when cells divide. (hindawi.com)
  • CUP1 is a nonessential gene that allows cells to grow in the presence of copper in a dosage-dependent manner. (genetics.org)
  • Also, each vector contains a combination Kanamycin/Neomycin antibiotic selection gene, which allows for selection in both E. coli and mammalian cells, while minimizing vector size so that transfection efficiency is maximally enhanced. (genlantis.com)
  • Inactivation of encapsulated cells and their therapeutic effects by means of TGL triple-fusion reporter/biosafety gene. (sigmaaldrich.com)
  • Altogether, these results show that TGL triple-fusion reporter gene may be a good starting point in the search of a suitable biosafety strategy to inactivate encapsulated cells and control their therapeutic effects. (sigmaaldrich.com)
  • Reporter genes are model systems used by researchers to assess various characteristics, such as delivery of an mRNA to a cell and percentage of cells transfected. (trilinkbiotech.com)
  • A chloramphenicol acetyltransferase reporter minigenome RNA was expressed either by T7 RNA polymerase in the cytoplasm of transfected cells or, alternatively, by RNA polymerase I in the nucleus. (asm.org)
  • No reporter gene expression could be detected in plasma cells and non-B cells of the hemopoietic system. (jimmunol.org)
  • Conversely, the loss of Pax5 expression by conditional gene inactivation converts committed pro-B cells with a restricted B lymphoid potential into lymphoid progenitors with a broad developmental potential ( 13 ). (jimmunol.org)
  • In two studies, the Pax5 gene is shown to be expressed in pre-pro-B cells already at ∼40% ( 5 ) or close to 100% ( 20 ) of the level observed in committed pro-B cells. (jimmunol.org)
  • Genomic organization of the human chondromodulin-1 gene containing a promoter region that confers the expression of reporter gene in chondrogenic ATDC5 cells. (sigmaaldrich.com)
  • We have explored the localization and developmental potential of cells fated to express SCL during murine development using SCL-lacZ mutant mice in which the Escherichia coli lacZ reporter gene was 'knocked in' to the SCL locus. (bloodjournal.org)
  • Although the gene ablation and gene expression studies provided strong circumstantial evidence for the role of SCL as a hematopoietic stem cell regulator, a functional correlation between endogenous SCL expression and progenitor cell activity in adult hematopoietic cells was first provided by the analysis of mice in which the Escherichia coli lacZ reporter gene had been "knocked in" to the SCL locus ( SCL-lacZ mice). (bloodjournal.org)
  • Host cell reactivation (HCR) of β-galactosidase activity for UV-irradiated Ad5MCMVlacZ was significantly reduced in non-irradiated CHO-UV61 cells compared to that in non-irradiated CHO-AA8 cells suggesting that repair in the transcribed strand of the UV-damaged reporter gene in untreated cells utilizes TCR. (portlandpress.com)
  • Prior UV-irradiation of cells with low UV fluences resulted in a transient enhancement of HCR for expression of the UV-damaged reporter gene in CHO-AA8 cells but not in TCR deficient CHO-UV61 cells. (portlandpress.com)
  • Transient transfection analysis of plasmids containing the 5′ upstream region of the human α -actin gene fused to human growth hormone or bacterial chloramphenicol acetyltransferase coding sequences into Rat-2 and ras -transformed Rat-2 (HO6) cells indicates that α -actin promoter is repressed in ras -transformed cells. (aacrjournals.org)
  • The expression of hNIS and GFP genes was confirmed in R NIS cells by flow cytometry and immunofluorescent staining. (snmjournals.org)
  • The human growth hormone (hGH) transient assay system described here is based on the expression of hGH directed by cells transfected with hGH fusion genes. (asm.org)
  • Separate cis-acting DNA elements of the mouse pro-alpha 1(I) collagen promoter direct expression of reporter genes to different type I collagen-producing cells in transgenic mice. (rupress.org)
  • To assess the potential use of PXR as a sensor for aquatic emerging pollutants, we employed an in vitro reporter gene assay (HG5LN-hPXR cells) to screen a panel of environmental chemicals and to assess PXR-active chemicals in (waste) water samples. (ebscohost.com)
  • In particular, reporter gene technologies have been employed to monitor gene expression levels, track cells to study biological development or cell transplantation therapy, and monitor gene therapy. (confex.com)
  • The reporter cell line was then screened with bioactive small molecule library, and a small molecule Tanshinone I was found to significantly inhibit the transcriptional activity of MMP12 gene in HEK293-MMP12-T2A-luciferase-KI cell line by luciferase activity assay, which was further confirmed to inhibit the expression of MMP12 mRNA in wild-type HEK293 cells. (ejbiotechnology.info)
  • NF-ĸB/SEAP reporter cells (IML-101) were plated in 96-well plates at 50,000 cells/well. (celltechnology.com)
  • Use of secreted alkaline phosphatase as a reporter of gene expression in mammalian cells. (celltechnology.com)
  • In order to avoid non-homologous recombination cells selecting, neomycin (Neo) resistant and thymidin kinase (TK) genes were included as positive and negative selectable markers, respectively. (sid.ir)
  • On account of the fact that the frequency of HR is low, thus Hematopoetic stem cells (HSC) should be expanded befor and after gene targeting by HR. In this study HSCs were also separated from cord blood and then to find out the high expansion level of the HSCs, different culture mediums with different growth factors were evaluated. (sid.ir)
  • SEAP Reporter Gene Assay Kit (Luminescence) provides a simple chemiluminescence method for the sensitive quantitation of SEAP (secreted alkaline phosphatase) in conditioned cell culture medium from transfected cells. (creativebiomart.net)
  • Compared to other conventional intracellular reporters such as chloramphenicol acetyltransferase (CAT) and firefly luciferase, SEAP has the advantage of being secreted from transfected cells into the culture medium. (creativebiomart.net)
  • We examined the effects of siRNA duplexes against the mutant alleles in allele-specific gene silencing and off-target silencing against the wild-type allele under heterozygous conditions, which were generated by cotransfecting the reporter alleles and siRNA duplexes into cultured human cells. (alliedacademies.org)
  • The siRNA duplexes having the potential to specifically suppress the expression of the mutant reporter allele consistently inhibited the expression of the bona fide mutant APP as well as amyloid 40- and 42-peptides in Cos-7 cells expressing both the full-length Swedish- and wild-type APP alleles. (alliedacademies.org)
  • Reporter gene (RG) imaging of cell-based therapies provides a direct readout of therapeutic efficacy by assessing the fate of implanted cells. (uc3m.es)
  • The hGH transient assay system is appropriate for analyses of regulation of gene expression and was utilized here to investigate the effect of the simian virus 40 enhancer on the herpes simplex virus thymidine kinase promoter and the effect of zinc on the mouse metallothionein-I promoter. (asm.org)
  • In molecular biology, a reporter gene (often simply reporter) is a gene that researchers attach to a regulatory sequence of another gene of interest in bacteria, cell culture, animals or plants. (wikipedia.org)
  • Luciferase Genes as Reporter Reactions: How to Use Them in Molecular Biology? (springerprofessional.de)
  • The aim of this chapter is to provide the reader with an overview of state-of-the-art bioluminescent tools based on luciferase genes, highlighting molecular biology strategies that have been applied so far, together with some selected examples. (springerprofessional.de)
  • Induction by HHV-6 was found to be dependent on the reporter construct used, because the CAT gene and, to a lesser extent, the HSV-1 thymidine kinase gene were responsive to HHV-6 infection although no significant activation of growth hormone constructs was observed. (microbiologyresearch.org)
  • Position effects are often more evident with smaller or mutated transgenic constructs where the reporter is presumably more exposed to interference from surrounding regulatory elements. (alpfmedical.info)
  • An example of a selectable-marker which is also a reporter in bacteria is the chloramphenicol acetyltransferase (CAT) gene, which confers resistance to the antibiotic chloramphenicol. (wikipedia.org)
  • In the case of selectable-marker reporters such as CAT, the transfected population of bacteria can be grown on a substrate that contains chloramphenicol. (wikipedia.org)
  • In this system, expression of a model minigenome RNA containing the chloramphenicol acetyltransferase (CAT) gene in the negative-sense orientation flanked by the conserved 5′- and 3′-terminal sequences of the THOV nucleoprotein (NP) gene segment, together with the three polymerase subunits (PA, PB1, and PB2) and the viral NP, leads to the formation of transcriptionally active vRNPs. (asm.org)
  • Reporter genes can be expressed constitutively or inducably with an external intervention such as the introduction of IPTG in the β-galactosidase system. (thermofisher.com)
  • the reporter gene can be expressed constitutively (that is, it is "always on") or inducibly with an external intervention such as the introduction of Isopropyl β-D-1-thiogalactopyranoside (IPTG) in the β-galactosidase system. (wikipedia.org)
  • To activate reporter genes, they can be expressed constitutively, where they are directly attached to the gene of interest to create a gene fusion. (wikipedia.org)
  • A stable luciferase reporter gene assay was developed based on the thyroid hormone responsive rat pituitary tumor GH3 cell line that constitutively expresses both thyroid hormone receptor isoforms. (wur.nl)
  • A beta-galactosidase gene under the control of a constitutively expressed beta-actin promoter was used as a transfection standard, and luciferase and beta gal expression were measured by a commercially available kit. (semanticscholar.org)
  • The advantages of this α -actin promoter-reporter gene assay system to screen for drugs that inhibit the transforming activity of ras , either directly or indirectly, are discussed. (aacrjournals.org)
  • Quantification of Atlantic salmon type-I interferon using an Mx1 promoter reporter gene assay. (semanticscholar.org)
  • article{Johansen2004QuantificationOA, title={Quantification of Atlantic salmon type-I interferon using an Mx1 promoter reporter gene assay. (semanticscholar.org)
  • The luciferase enzyme catalyzes a reaction with its substrate (usually luciferin) to produce yellow-green or blue light, depending on the luciferase gene. (thermofisher.com)
  • The bacterial lacZ gene encodes a β-galactosidase enzyme. (thermofisher.com)
  • This enzyme causes bacteria expressing the gene to appear blue when grown on a medium that contains the substrate analog X-gal. (wikipedia.org)
  • The restriction enzyme map and nucleotide sequence revealed the human ChM-1 gene consisting of seven exons and exon-intron boundaries. (sigmaaldrich.com)
  • Reporter enzyme activities following growth of these recombinants in complex medium (LBmc) and in minimal medium with glucose or succinate as the sole carbon source allowed the identification of genes highly expressed under one or more growth condition and those expressed at very low to background levels. (asm.org)
  • A novel secreted bioluminescent reporter enzyme. (springerprofessional.de)
  • Reporter genes can also serve as controls for transfection. (thermofisher.com)
  • For example, transfection efficiencies between different experiments can be normalized by comparing the expression levels of a reporter gene used in all of the experiments. (thermofisher.com)
  • Many methods of transfection and transformation - two ways of expressing a foreign or modified gene in an organism - are effective in only a small percentage of a population subjected to the techniques. (wikipedia.org)
  • Control siRNAs for commonly used reporter systems (GFP, Luciferase, etc.) have been experimentally validated for functionality, and the TOX transfection control can induce cell death for use as a positive control in viability studies. (horizondiscovery.com)
  • They are usually used to study the gene expression or to act as a control for transfection efficiencies. (databridgemarketresearch.com)
  • Northern analysis showed that the mRNA level of a related, endogenous auxin-inducible GST gene of Arabidopsis was increased in some of these mutants as well. (tudelft.nl)
  • To explore the transcriptional regulation of MMP12 gene, a sensitive luciferase reporter HEK293 cell line for endogenous MMP12 promoter was generated by CRISPR/Cas9 technology. (ejbiotechnology.info)
  • The transgene and the endogenous gene were developmentally induced in young cotyledons to a level higher than in older cotyledons and leaves. (deepdyve.com)
  • Antimycin A (AA), a mitochondrial electron transport chain inhibitor, and monofluroacetate (MFA), a TCA cycle inhibitor, induced expression of the transgene and the endogenous gene in parallel. (deepdyve.com)
  • induced expression of the transgene and the endogenous gene in parallel. (deepdyve.com)
  • For this purpose, the androgen receptor (AR) expressing human breast cancer cell line T47D was stably transfected with a luciferase gene under transcriptional control of the PB-ARE-2 androgen response element. (tudelft.nl)
  • lacZ transcriptional fusion into the genome of SS9, replacing the wild-type ompH gene, generating strain EC10. (asm.org)
  • In this paper, we reviewed noninvasive molecular imaging systems for the monitoring of the biogenesis of microRNAs and post-transcriptional regulation of genes by microRNAs and the roles of microRNAs in various biological processes. (thno.org)
  • Homologous recombination of the DNA fragments cloned in pTH1522 into the S. meliloti genome generates transcriptional fusions to either the reporter genes gfp + and lacZ or gusA and rfp , depending on the orientation of the cloned fragment. (asm.org)
  • This novel luciferase knock-in reporter system will be helpful for investigating the transcriptional regulation of MMP12 gene and screening the drugs targeting MMP12 gene. (ejbiotechnology.info)
  • Transcriptional activation of CYP gene expression by xenobiotics may have fundamental effects on body physiology. (surrey.ac.uk)
  • They are ideally not present in the native genome to be able to isolate reporter gene expression as a result of the gene of interest's expression. (wikipedia.org)
  • Microbial genome sequences have revealed a multitude of genes for which functional information is almost completely lacking. (asm.org)
  • One can infer that the functions of these genes are diverse, and hence, at a whole-genome level, it would seem prudent to adopt multiple approaches to the identification of gene functions. (asm.org)
  • The sequencing results indicated that one allele of the genome was proven to have a site-directed insertion of luciferase gene and another allele of the genome was confirmed to have additional 48 bp insertion in this cell line. (ejbiotechnology.info)
  • HR replace the damaged gene with wild type, without causing any changes elsewhere in the genome. (sid.ir)
  • A 1.4-kb human a1-PI 5'-flanking sequence fused to the E. coli LacZ gene was used to generate transgenic mice. (arvojournals.org)
  • Here we show that the presence of G4 motifs within maize LTRs results in a markedly reduced expression of the downstream located lacZ gene in yeast compared to a similar sequence with mutations preventing quadruplex formation. (biomedcentral.com)
  • Gene sequencing reveals that this tumor medulloblastoma or MB posse. (bio-medicine.org)
  • Gene sequencing reveals that this tumor, medulloblastoma, or MB, possesses far fewer genetic abnormalities than comparable adult tumors. (bio-medicine.org)
  • Imaging prostate tumor growth and lymph node metastasis in nude mice therefore becomes possible using this new fluorescent gene reporter. (spie.org)
  • The reporter gene construct contains one or more gene regulatory elements being analyzed, the structural sequence of the reporter gene, and the sequences required for the formation of functional mRNA. (thermofisher.com)
  • Transgenic arabidopsis plants were isolated that contained a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco was fused to the kanamycin resistance (nptII) as well as to the β-glucuronidase (gusA) reporter gene. (tudelft.nl)
  • To introduce a reporter gene into an organism, scientists place the reporter gene and the gene of interest in the same DNA construct to be inserted into the cell or organism. (wikipedia.org)
  • In building the DNA construct, a segment of DNA coding for a flexible polypeptide linker region is usually included so that the reporter and the gene product will only minimally interfere with one another. (wikipedia.org)
  • In the present study, in order to evaluation of gene targeting by HR in Beta globin locus, a specific construct was designed. (sid.ir)
  • To facilitate HR process the upstream (US) and the downstream (DS) of beta-globin gene were included within construct. (sid.ir)
  • You can then transform your reporter construct into your plant species of choice to monitor its expression. (bitesizebio.com)
  • Two forms of ectopic expression from a transgenic reporter are possible: Inconsistent ectopic expression owing to position effects is discernible by the comparison of a number of transgenic animals carrying the same construct. (alpfmedical.info)
  • These studies demonstrate that hRluc should be a useful primary reporter gene with high sensitivity when used alone or in conjunction with other bioluminescence reporter genes for imaging in living rodents. (spiedigitallibrary.org)
  • Most current molecular-genetic imaging strategies are based on a reporter gene and a complementary reporter probe. (snmjournals.org)
  • By contrast, genetic reporter signals are amplified by cell division. (elifesciences.org)
  • In this study, we aimed to develop a switch to turn this genetic reporter "on" or "off" while monitoring cell grafts via MRI. (genscript.com)
  • The genetic reporter FTH1 can thus be switched "on" or "off" throughout longitudinal monitoring of cell grafts, limiting expression to when MRI contrast is needed. (genscript.com)
  • Taken altogether, the data provide genetic evidence that suggests that induction of the AtAOX1a gene by distinct mitochondrial perturbations are via distinct, but overlapping signaling pathways that are tissue specific. (deepdyve.com)
  • With some simple genetic techniques, one can attach the promoter of the gene you want to investigate to the GUS coding region. (bitesizebio.com)
  • In a study led by Eric Gamazon , PhD, research instructor in Medicine at Vanderbilt, and Ayellet Segrè, PhD, of Harvard University, the team developed computational approaches to integrate the two types of data and link disease-associated genetic variants to gene expression in a broad collection of human tissues. (vumc.org)
  • This is the most comprehensive database of gene expression across many tissues, and it is a unique resource for understanding what disease- and trait-associated genetic variants are doing," Gamazon said. (vumc.org)
  • The investigators found that about 60 percent of known disease- and trait-associated genetic variants associate with genetic variants that influence gene expression, with more than half of the variants regulating two or more genes. (vumc.org)
  • What we're finding though is that by 'zooming in' on those variants that actually control the expression of genes in relevant tissues, we are enriching for novel, previously unidentified disease-associated genetic variants. (vumc.org)
  • They can be fused to regulatory sequences or genes of interest to report expression location or levels. (nature.com)
  • However, some of the regulatory mechanisms of the CXCL12 gene are still unclear. (vde-verlag.de)
  • Our study reveals a regulatory pathway for the CXCL12 gene involved in microRNAs, providing a theoretical basis for the clinical treatment of gastric cancer and the development of new drugs. (vde-verlag.de)
  • 18 Of course, this view of the role of SCL may be oversimplified, as the complete molecular program of hematopoietic commitment is likely to require a complex, interdependent network of regulatory genes. (bloodjournal.org)
  • Consistent ectopic expression owing to regulatory interactions within the reporter gene per se is less clear-cut, since they may only be apparent in a proportion of the analyzed samples (if dependent on expression levels, and so forth). (alpfmedical.info)
  • Glow luminescence reporter gene assay combining benefits of both bright signal and long-lasting signal for firefly luciferase. (perkinelmer.com)
  • Now researchers have several good options for taking advantage of dual-reporter formats with stable, long-lived luminescence reporters that emit at different wavelengths. (bmglabtech.com)
  • MicroRNAs are small noncoding RNAs regulating gene expression, through base paring with their target mRNAs, which have been actively investigated as key regulators in a wide range of biological processes. (thno.org)
  • The two functional parts of a gene include the coding region and the promoter region. (thermofisher.com)
  • This unit describes quantitation of functional interactions between HIV envelope glcoprotein and target cell receptors, using assay of cell fusion‐dependent reporter gene activation. (currentprotocols.com)
  • Then human PCa PC3 cell lines expressing iRFP gene reporter were orthotopically implanted in male Nu/Nu mice at 8-10 weeks old. (spie.org)
  • From my PhD, I mainly learnt that transgenic reporter mice may open the window to the full spectrum of molecular mechanism assessment in a whole-body context, now that I'm looking for a system-biology postdoc, I'm happy to realize that my experience with reporter mice can be so widely turned in different fields. (reportergene.com)
  • Here we assess the usefulness of electroporation for enhancing gene transfer in the lungs of mice and sheep. (cfgenetherapy.org.uk)
  • CONCLUSIONS: These results indicate that electroporation can be used to enhance gene transfer in the lungs of mice and sheep without compromising the duration of expression. (cfgenetherapy.org.uk)
  • North America is expected to account for the largest share of the reporter gene assay market in 2019, followed by Europe. (express-press-release.net)
  • Global Reporter Gene Assay Market is expected to rise from its initial estimated value of USD 1.44 billion to an estimated value of USD 3.15 billion by 2026, registering a CAGR of 10.25% in the forecast period of 2019-2026. (databridgemarketresearch.com)
  • Global reporter gene assay market is highly fragmented and the major players have used various strategies such as new product launches, expansions, agreements, joint ventures, partnerships, acquisitions, and others to increase their footprints in this market. (databridgemarketresearch.com)
  • Finally, the team found that humans with mutation of a key gene for this co-transmission were 10 times more vulnerable to very severe forms of addiction. (mcgill.ca)
  • Geneticist Dr. Hung Lo of MIT has not only discovered a way to remove and repair defective genes responsible for cancer and a host of many incurable diseases, the brilliant young scientist has also discovered a means to locate and repair the gene mutation responsible for CNN reporters. (impiousdigest.com)
  • Having a mother or daughter who carries a mutation on the BRCA1 or 2 genes puts women at an increased risk of developing breast and ovarian cancer. (washingtontimesreporter.com)
  • Choose from siSTABLE-modified controls, siRNA for silencing reporter genes (GFP, Luciferase, etc.) or other experimental needs. (horizondiscovery.com)
  • The present data suggest that the system with reporter alleles may permit the preclinical assessment of siRNA duplexes conferring ASP-RNAi, and thus contribute to the design and selection of the most suitable of such siRNA duplexes. (alliedacademies.org)
  • I highly recommend utilizing the GUS gene reporter system: if you want to track down individual gene expression and can generate some transgenic plants. (bitesizebio.com)
  • promoter situated immediately upstream of the reporter gene. (bio.net)
  • To study this response, called mitochondrial retrograde regulation, and developmental gene expression, a transgenic Arabidopsis thaliana (Col-0) line containing a firefly luciferase gene controlled by a promoter region of the Arabidopsis alternative oxidase 1a gene (AtAOX1a) was created. (deepdyve.com)
  • Arabidopsis alternative oxidase 1a gene ( AtAOX1a ) was created. (deepdyve.com)
  • Such genes are called reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because they are selectable markers. (wikipedia.org)
  • An emerging MRI reporter, ferritin heavy chain (FTH1), is recently applied to enhance the contrast and increase the sensitivity of MRI in the monitoring of solid tumors. (hindawi.com)
  • It has been reported that ferritin gene, an emerging MRI reporter gene, can enhance the contrast and increase the sensitivity of MRI [ 9 - 11 ]. (hindawi.com)
  • Multimodality imaging using noncytotoxic triple fusion (TF) reporter genes is an important application for cell-based tracking, drug screening, and therapy. (hindawi.com)
  • The Passive Lysis Solution (PLS) is a mammalian cell lysis solution intended to be used to prepare cell lysates for measurement of luciferase activity with the twinlite and sensilite reporter gene assay systems. (perkinelmer.com)
  • The aim of the work described in this report is to develop and characterize a cell-based androgen reporter assay. (tudelft.nl)
  • Reporter genes are often used as an indication of whether a certain gene has been taken up by or expressed in the cell or organism population. (wikipedia.org)
  • It is important to use a reporter gene that is not natively expressed in the cell or organism under study, since the expression of the reporter is being used as a marker for successful uptake of the gene of interest. (wikipedia.org)
  • Reporter genes can be used to assay for the activity of a particular promoter in a cell or organism. (wikipedia.org)
  • In this study, we have investigated the expression of Pax5 at single-cell resolution by inserting a GFP or human Cd2 indicator gene under the translational control of an internal ribosomal entry sequence into the 3′ untranslated region of Pax5 . (jimmunol.org)
  • At the molecular level, Pax5 fulfills its commitment function by repressing B cell lineage-inappropriate genes and activating B cell-specific genes, which results in the shutdown of inappropriate signaling systems and the simultaneous promotion of B lymphoid signal transduction ( 15 , 16 , 17 ). (jimmunol.org)
  • 2 3 SCL was also critically required for the development of hematopoiesis in the adult mouse 4 5 and for the induction of hematopoietic genes during embryonic stem cell differentiation. (bloodjournal.org)
  • In addition, stable rat fibroblast cell lines expressing human growth hormone or β -galactosidase under the control of α -actin promoter exhibit repressed reporter gene activity following transformation by the ras oncogene. (aacrjournals.org)
  • The resulting reporter cell lines responded in a very sensitive way to the stimulation of various test receptors. (lu.se)
  • Inhibition of TNFα activity by a quinazoline TNFα inhibitor (EMD Millipore Cat# 545380-34-5) with NF-ĸB/SEAP stable reporter cell line. (celltechnology.com)
  • This is done by linking the unknown promoter sequence to an easily detectable reporter gene whose product can be easily detected and quantifiably measured. (thermofisher.com)
  • Acoustic reporter genes (ARGs) overcome these limitations by using gas vesicle reporters that are detectable by an inexpensive and widely available imaging platform: ultrasound. (addgene.org)
  • Tools for conditional induction of gene expression in C. elegans are limited compared to other organisms. (nature.com)
  • Reporter genes can also be expressed by induction during growth. (wikipedia.org)
  • It is concluded that the PXR reporter gene assay is a reliable and complementary method to assess the CYP3A4 induction potential of drugs and other xenobiotics. (aspetjournals.org)
  • The reporter system was termed HighTRACE® (high-throughput reporter assay with clone election). (lu.se)
  • Several noninvasive imaging modalities such as positron emission tomography (PET), single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), and optical imaging are available for real-time repetitive imaging of reporter gene expression in living subjects [ 1 - 4 ]. (hindawi.com)
  • Optical imaging is quick and cost-effective and requires no radioisotopes for validating different reporter systems. (hindawi.com)
  • However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. (emka.si)
  • This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. (emka.si)
  • Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. (emka.si)
  • This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. (emka.si)
  • The aim of this study was to investigate the feasibility of nuclear molecular imaging using the human sodium iodide symporter (hNIS) as a reporter gene to monitor macrophage migration toward the inflammatory foci. (snmjournals.org)
  • These data support the feasibility of hNIS reporter gene imaging to monitor the macrophage migration toward an inflammatory lesion. (snmjournals.org)
  • NIS reporter gene imaging has the advantages of using known radiotracers such as 99m Tc-pertechnetate and radioiodine. (snmjournals.org)
  • Lee SW, Lee SH, Biswal S. Magnetic Resonance Reporter Gene Imaging. (thno.org)
  • Furthermore, reporter gene imaging plays a central role in this field. (thno.org)
  • Compared with the other techniques, magnetic resonance (MR)-based reporter gene imaging has not occupied center stage, despite its superior three-dimensional depictions of anatomical details. (thno.org)
  • In this article, the authors review the principles and applications of various types of MR reporter gene imaging technologies and discuss their advantages and disadvantages. (thno.org)
  • Reporter gene imaging has primarily used optical imaging and radionuclide imaging modalities, but these modalities have limitations such as a shallow depth of view for optical imaging, the use of ionizing radiation for radionuclide imaging, and poor spatial resolution for both imaging modalities. (confex.com)
  • Magnetic resonance imaging (MRI) has also been used for reporter gene imaging, but current MRI methods are notoriously insensitive for this application. (confex.com)
  • Limited Depth of Signal/Imaging: Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal tract. (addgene.org)
  • arg1 and lux reporters both allow for visualization of the bacteria, but lux imaging shows only that the bacteria localize to somewhere in the mouse abdomen. (addgene.org)
  • Development of stable reporter system cloning luxCDABE genes into chromosome of Salmonella enterica serotypes using Tn7 transposon. (biomedsearch.com)