A species of parasitic OOMYCETES in the family Peronosporaceae that is the causative agent of late blight of potato.
A genus of destructive parasitic OOMYCETES in the family Peronosporaceae, order Peronosporales, affecting numerous fruit, vegetable, and other crops. Differentiation of zoospores usually takes place in the sporangium and no vesicle is formed. It was previously considered a fungus.
A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Diseases of plants.
Sexual union of a male and a female in non-human species.
Eukaryotes in the group STRAMENOPILES, formerly considered FUNGI, whose exact taxonomic level is unsettled. Many consider Oomycetes (Oomycota) a phylum in the kingdom Stramenopila, or alternatively, as Pseudofungi in the phylum Heterokonta of the kingdom Chromista. They are morphologically similar to fungi but have no close phylogenetic relationship to them. Oomycetes are found in both fresh and salt water as well as in terrestrial environments. (Alexopoulos et al., Introductory Mycology, 4th ed, pp683-4). They produce flagellated, actively motile spores (zoospores) that are pathogenic to many crop plants and FISHES.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
A publication issued at stated, more or less regular, intervals.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The collection, writing, and editing of current interest material on topics related to biomedicine for presentation through the mass media, including newspapers, magazines, radio, or television, usually for a public audience such as health care consumers.
The general name for a group of fat-soluble pigments found in green, yellow, and leafy vegetables, and yellow fruits. They are aliphatic hydrocarbons consisting of a polyisoprene backbone.
An enzyme that catalyzes the condensation of two molecules of geranylgeranyl diphosphate to give prephytoene diphosphate. The prephytoene diphosphate molecule is a precursor for CAROTENOIDS and other tetraterpenes.
A large order of insects characterized by having the mouth parts adapted to piercing or sucking. It is comprised of four suborders: HETEROPTERA, Auchenorrhyncha, Sternorrhyncha, and Coleorrhyncha.
Oxygenated forms of carotenoids. They are usually derived from alpha and beta carotene.
A member of the AGARICALES known for edible MUSHROOMS.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Coloration or discoloration of a part by a pigment.
The book composed of writings generally accepted by Christians as inspired by God and of divine authority. (Webster, 3d ed)
The measure of that part of the heat or energy of a system which is not available to perform work. Entropy increases in all natural (spontaneous and irreversible) processes. (From Dorland, 28th ed)
Individual or group aggressive behavior which is socially non-acceptable, turbulent, and often destructive. It is precipitated by frustrations, hostility, prejudices, etc.
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
The relationships of groups of organisms as reflected by their genetic makeup.
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
A behavioral response manifested by leaving home in order to escape from threatening situations. Children or adolescents leaving home without permission is usually implied.
Dermatological pruritic lesion in the feet, caused by Trichophyton rubrum, T. mentagrophytes, or Epidermophyton floccosum.
Runaway and homeless children and adolescents living on the streets of cities and having no fixed place of residence.
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.
A product of hard secondary xylem composed of CELLULOSE, hemicellulose, and LIGNANS, that is under the bark of trees and shrubs. It is used in construction and as a source of CHARCOAL and many other products.
Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.
A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
A family of fungi, order POLYPORALES, found on decaying wood.
A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.

SWM1, a developmentally regulated gene, is required for spore wall assembly in Saccharomyces cerevisiae. (1/10689)

Meiosis in Saccharomyces cerevisiae is followed by encapsulation of haploid nuclei within multilayered spore walls. Formation of this spore-specific wall requires the coordinated activity of enzymes involved in the biosynthesis of its components. Completion of late events in the sporulation program, leading to spore wall formation, requires the SWM1 gene. SWM1 is expressed at low levels during vegetative growth but its transcription is strongly induced under sporulating conditions, with kinetics similar to those of middle sporulation-specific genes. Homozygous swm1Delta diploids proceed normally through both meiotic divisions but fail to produce mature asci. Consistent with this finding, swm1Delta mutant asci display enhanced sensitivity to enzymatic digestion and heat shock. Deletion of SWM1 specifically affects the expression of mid-late and late sporulation-specific genes. All of the phenotypes observed are similar to those found for the deletion of SPS1 or SMK1, two putative components of a sporulation-specific MAP kinase cascade. However, epistasis analyses indicate that Swm1p does not form part of the Sps1p-Smk1p-MAP kinase pathway. We propose that Swm1p, a nuclear protein, would participate in a different signal transduction pathway that is also required for the coordination of the biochemical and morphological events occurring during the last phase of the sporulation program.  (+info)

NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. (2/10689)

A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. Molecular genetic analysis of NMD3 revealed that it is an essential gene required for stable 60S ribosomal subunits. Cells bearing a temperature-sensitive allele of NMD3 had decreased levels of 60S subunits at the nonpermissive temperature which resulted in the formation of half-mer polysomes. Pulse-chase analysis of rRNA biogenesis indicated that 25S rRNA was made and processed with kinetics similar to wild-type kinetics. However, the mature RNA was rapidly degraded, with a half-life of 4 min. Nmd3p fractionated as a cytoplasmic protein and sedimented in the position of free 60S subunits in sucrose gradients. These results suggest that Nmd3p is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. Putative orthologs of Nmd3p exist in Drosophila, in nematodes, and in archaebacteria but not in eubacteria. The Nmd3 protein sequence does not contain readily recognizable motifs of known function. However, these proteins all have an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization.  (+info)

Characterization and expression of the cDNA encoding a new kind of phospholipid transfer protein, the phosphatidylglycerol/phosphatidylinositol transfer protein from Aspergillus oryzae: evidence of a putative membrane targeted phospholipid transfer protein in fungi. (3/10689)

The full-length cDNA of a phospholipid transfer protein (PLTP) was isolated from Aspergillus oryzae by a RACE-PCR procedure using degenerated primer pool selected from the N-terminal sequence of the purified phosphatidylinositol/phosphatidylglycerol transfer protein (PG/PI-TP). The cDNA encodes a 173 amino acid protein of 18823 Da. The deduced amino acid sequence from position 38 to 67 is 100% identical to the N-terminal sequence (first 30 amino acids) of the purified PG/PI-TP. This amino acid sequence is preceded by a leader peptide of 37 amino acids which is predicted to be composed of a signal peptide of 21 amino acids followed by an extra-sequence of 16 amino acids, or a membrane anchor protein signal (amino acid 5-29). This strongly suggests that the PG/PI-TP is a targeted protein. The deduced mature protein is 138 amino acids long with a predicted molecular mass of 14933 Da. Comparison of the deduced PG/PI-TP sequence with other polypeptide sequences available in databases revealed a homology with a protein deduced from an open reading frame coding for an unknown protein in Saccharomyces cerevisiae (36% identity and 57% similarity). Apart from this homology, the PG/PI-TP is unique and specific to the filamentous fungi on the basis of comparison of PLTP protein sequences. Northern blot analysis of RNA isolated from A. oryzae cultures grown on glucose or glucose supplemented with phospholipids suggests that the PG/PI-TP is transcribed by only one RNA species and allows us to show that expression of the protein is regulated at the messenger RNA level.  (+info)

Rpp14 and Rpp29, two protein subunits of human ribonuclease P. (4/10689)

In HeLa cells, the tRNA processing enzyme ribonuclease P (RNase P) consists of an RNA molecule associated with at least eight protein subunits, hPop1, Rpp14, Rpp20, Rpp25, Rpp29, Rpp30, Rpp38, and Rpp40. Five of these proteins (hPop1p, Rpp20, Rpp30, Rpp38, and Rpp40) have been partially characterized. Here we report on the cDNA cloning and immunobiochemical analysis of Rpp14 and Rpp29. Polyclonal rabbit antibodies raised against recombinant Rpp14 and Rpp29 recognize their corresponding antigens in HeLa cells and precipitate catalytically active RNase P. Rpp29 shows 23% identity with Pop4p, a subunit of yeast nuclear RNase P and the ribosomal RNA processing enzyme RNase MRP. Rpp14, by contrast, exhibits no significant homology to any known yeast gene. Thus, human RNase P differs in the details of its protein composition, and perhaps in the functions of some of these proteins, from the yeast enzyme.  (+info)

The Saccharomyces cerevisiae CWH8 gene is required for full levels of dolichol-linked oligosaccharides in the endoplasmic reticulum and for efficient N-glycosylation. (5/10689)

The Saccharomyces cerevisiae mutant cwh8 was previously found to have an anomalous cell wall. Here we show that the cwh8 mutant has an N -glycosylation defect. We found that cwh8 cells were resistant to vanadate and sensitive to hygromycin B, and produced glycoforms of invertase and carboxypeptidase Y with a reduced number of N -chains. We have cloned the CWH8 gene. We found that it was nonessential and encoded a putative transmembrane protein of 239 amino acids. Comparison of the in vitro oligosaccharyl transferase activities of membrane preparations from wild type or cwh8 Delta cells revealed no differences in enzyme kinetic properties indicating that the oligosaccharyl transferase complex of mutant cells was not affected. cwh8 Delta cells also produced normal dolichols and dolichol-linked oligosaccharide intermediates including the full-length form Glc3Man9GlcNAc2. The level of dolichol-linked oligosaccharides in cwh8 Delta cells was, however, reduced to about 20% of the wild type. We propose that inefficient N -glycosylation of secretory proteins in cwh8 Delta cells is caused by an insufficient supply of dolichol-linked oligosaccharide substrate.  (+info)

Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers. (6/10689)

The identification and classification of yeasts have traditionally been based on morphological, physiological and biochemical traits. Various kits have been developed as rapid systems for yeast identification, but mostly for clinical diagnosis. In recent years, different molecular biology techniques have been developed for yeast identification, but there is no available database to identify a large number of species. In the present study, the restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene were used to identify a total of 132 yeast species belonging to 25 different genera, including teleomorphic and anamorphic ascomycetous and basidiomycetous yeasts. In many cases, the size of the PCR products and the restriction patterns obtained with endonucleases CfoI, HaeIII and HinfI yielded a unique profile for each species. Accordingly, the use of this molecular approach is proposed as a new rapid and easy method of routine yeast identification.  (+info)

The genes for the Golgi apparatus N-acetylglucosaminyltransferase and the UDP-N-acetylglucosamine transporter are contiguous in Kluyveromyces lactis. (7/10689)

The mannan chains of Kluyveromyces lactis mannoproteins are similar to those of Saccharomyces cerevisiae except that they lack mannose phosphate and have terminal alpha(1-->2)-linked N-acetylglucosamine. Previously, Smith et al. (Smith, W. L. Nakajima, T., and Ballou, C. E. (1975) J. Biol. Chem. 250, 3426-3435) characterized two mutants, mnn2-1 and mnn2-2, which lacked terminal N-acetylglucosamine in their mannoproteins. The former mutant lacks the Golgi N-acetylglucosaminyltransferase activity, whereas the latter one was recently found to be deficient in the Golgi UDP-GlcNAc transporter activity. Analysis of extensive crossings between the two mutants led Ballou and co-workers (reference cited above) to conclude that these genes were allelic or tightly linked. We have now cloned the gene encoding the K. lactis Golgi membrane N-acetylglucosaminyltransferase by complementation of the mnn2-1 mutation and named it GNT1. The mnn2-1 mutant was transformed with a 9.5-kilobase (kb) genomic fragment previously shown to contain the gene encoding the UDP-GlcNAc transporter; transformants were isolated, and phenotypic correction was monitored after cell surface labeling with fluorescein isothiocyanate-conjugated Griffonia simplicifolia II lectin, which binds terminal N-acetylglucosamine, and a fluorescence-activated cell sorter. The above 9.5-kb DNA fragment restored the wild-type lectin binding phenotype of the transferase mutant; further subcloning of this fragment yielded a smaller one containing an opening reading frame of 1,383 bases encoding a protein of 460 amino acids with an estimated molecular mass of 53 kDa, which also restored the wild-type phenotype. Transformants had also regained the ability to transfer N-acetylglucosamine to 3-0-alpha-D-mannopyranosyl-D-mannopyranoside. The gene encoding the above transferase was found to be approximately 1 kb upstream from the previously characterized MNN2 gene encoding the UDP-GlcNAc Golgi transporter. Each gene can be transcribed independently by their own promoter. To our knowledge this is the first demonstration of two Golgi apparatus functionally related genes being contiguous in a genome.  (+info)

The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance. (8/10689)

The mdm17 mutation causes temperature-dependent defects in mitochondrial inheritance, mitochondrial morphology, and the maintenance of mitochondrial DNA in the yeast Saccharomyces cerevisiae. Defects in mitochondrial transmission to daughter buds and changes in mitochondrial morphology were apparent within 30 min after shifting cells to 37 degrees C, while loss of the mitochondrial genome occurred after 4-24 h at the elevated temperature. The mdm17 lesion mapped to MGM1, a gene encoding a dynamin-like GTPase previously implicated in mitochondrial genome maintenance, and the cloned MGM1 gene complements all of the mdm17 mutant phenotypes. Cells with an mgm1-null mutation displayed aberrant mitochondrial inheritance and morphology. A version of mgm1 mutated in a conserved residue in the putative GTP-binding site was unable to complement any of the mutant defects. It also caused aberrant mitochondrial distribution and morphology when expressed at high levels in cells that also contained a wild-type copy of the gene. Mgm1p was localized to the mitochondrial outer membrane and fractionated as a component of a high molecular weight complex. These results indicate that Mgm1p is a mitochondrial inheritance and morphology component that functions on the mitochondrial surface.  (+info)

In my experience, DNA purified from agarose gels contains transformation poisons. I once demonstrated this by gel purifying covalent closed circular plasmid. The gel-purified DNA yielded 100-1000X fewer transformants/ug (depending on isolation technique). Youll get higher cloning efficiency if you purify your genomic digest on sucrose gradients and just analyze small aliquots to find the desired size fraction. If you can, avoid gel purification of the vector also, so much the better. Regards, John Thompson Gianluca Molla ,molla at imiucca.csi.unimi.it, wrote: ,Hello to everyone. ,Is the first time I write to this NG although I read ,the posts for more than one year. ,In our lab we are trying to construct a genomic library ,from a yeast (R. gracilis). We extract genomic DNA, ,digest it with EcoRI, run the digestion fragments on an ,agarose gel, extract the DNA from 7.5 to 9 kb from the gel (this is the ,size of fragments we are interested in) and ligate them to a cloning ,vector. The number of ...
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
The development of high-throughput and large-scale technologies have expanded the screening capacity for human-yeast complementation pairs. As a result, several systematic screens have reported testing the essential yeast genes for replaceability (Zhang et al. 2003; Hamza et al. 2015; Kachroo et al. 2015; Sun et al. 2016; Yang et al. 2017; Garge et al. 2019; Laurent et al. 2019). These studies generated overlapping lists of human-yeast complementation pairs and arrived at similar conclusions regarding features that predict the replaceability of essential yeast genes. However, compared to the essential yeast genes, the nonessential genes are a much larger set and have a variety of different phenotypic readouts, making them more difficult to screen systematically for complementation. In this study, we have started this process by focusing on a subset of the nonessential yeast genes, specifically those required for chromosome maintenance. We identified 20 complementation pairs that are replaceable ...
The Saccharomyces cerevisiae SNF2 gene affects the expression of many diversely regulated genes and has been implicated in transcriptional activation. We report here the cloning and characterization of STH1, a gene that is homologous to SNF2. STH1 is essential for mitotic growth and is functionally distinct from SNF2. A bifunctional STH1-beta-galactosidase protein is located in the nucleus. The predicted 155,914-Da STH1 protein is 72% identical to SNF2 over 661 amino acids and 46% identical over another stretch of 66 amino acids. Both STH1 and SNF2 contain a putative nucleoside triphosphate-binding site and sequences resembling the consensus helicase motifs. The large region of homology shared by STH1 and SNF2 is conserved among other eukaryotic proteins, and STH1 and SNF2 appear to define a novel family of proteins related to helicases. ...
ID YEP367 preliminary; circular DNA; SYN; 8400 BP. XX AC ATCC37735; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE Saccharomyces/E.coli plasmid vector YEp367 - incomplete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC YEp352E from YEp352 & linker RC YEp363A from pNM480 & YEp351 RC YEp353A from pNM480 & YEp352 RC YEp353 from YEp353A & YEp352E RC YEp354A from pNM481 & YEp352 RC YEp354 from YEp354A & YEp352E RC YEp355A from pNM482 & YEp352 RC YEp355 from YEp355A & YEp352E RC YEp356, YEp356R from YEp353 & pUC18 RC YEp357, YEp357R from YEp354 & pUC18 RC YEp358, YEp358R from YEp355 & pUC18 RC YEp363 from YEp363A & YEp353 RC YEp364 from YEp363A & YEp354 RC YEp365 from YEp363A & YEp355 RC YEp366 from YEp363A & YEp356 RC YEp367 from YEp363A & YEp357 RC YEp368 from YEp363A & YEp358 RC YEp366R from YEp363A & YEp356R RC YEp367R from YEp363A & YEp357R RC YEp368R from YEp363A & YEp358R RC YIp353 from YEp353 & ...
ID YEP353 preliminary; circular DNA; SYN; 7944 BP. XX AC U03500; ATCC37725; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE Saccharomyces/E.coli plasmid vector YEp353 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RP 1-7944 RC YEp352E from YEp352 & linker RC YEp363A from pNM480 & YEp351 RC YEp353A from pNM480 & YEp352 RC YEp353 from YEp353A & YEp352E RC YEp354A from pNM481 & YEp352 RC YEp354 from YEp354A & YEp352E RC YEp355A from pNM482 & YEp352 RC YEp355 from YEp355A & YEp352E RC YEp356, YEp356R from YEp353 & pUC18 RC YEp357, YEp357R from YEp354 & pUC18 RC YEp358, YEp358R from YEp355 & pUC18 RC YEp363 from YEp363A & YEp353 RC YEp364 from YEp363A & YEp354 RC YEp365 from YEp363A & YEp355 RC YEp366 from YEp363A & YEp356 RC YEp367 from YEp363A & YEp357 RC YEp368 from YEp363A & YEp358 RC YEp366R from YEp363A & YEp356R RC YEp367R from YEp363A & YEp357R RC YEp368R from YEp363A & YEp358R RC YIp353 ...
TY - JOUR. T1 - The Saccharomyces cerevisiae gene SDS22 encodes a potential regulator of the mitotic function of yeast type 1 protein phosphatase. AU - MACKELVIE, SARAH H. AU - ANDREWS, PAUL D.. AU - STARK, MICHAEL J. R. PY - 1995/7. Y1 - 1995/7. N2 - In higher eukaryotes, the activity and specificity of the type 1 protein serine-threonine phosphatase (PP1) catalytic subunit is thought to be controlled by its association with a number of regulatory or targeting subunits. Here we describe the characterization of a gene encoding one such potential polypeptide in the yeast Saccharomyces cerevisiae. The gene which we have isolated (termed SDS22) encodes a product with a high degree of sequence identity to the fission yeast sds22 protein, a known regulator of the mitotic function of PP1 in Schizosaccharomyces pombe. Using two different criteria, we have demonstrated that Sds22p and the catalytic subunit of PP1 (Glc7p) interact in yeast cells. We have also generated a temperature-sensitive allele of ...
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We have isolated STN1, an essential Saccharomyces cerevisiae gene, as a suppressor of the cdc13-1 mutation. A synthetic lethal interaction between a temperature-sensitive mutant allele of STN1, stn1-13, and cdc13-1 was observed. Stn1 and Cdc13 proteins displayed a physical interaction by two-hybrid analysis. As shown previously for cdc13-1, stn1-13 cells at the restrictive temperature accumulate single-stranded DNA in subtelomeric regions of the chromosomes, but to a lesser extent than cdc13-1 cells. In addition, both Cdc13 and Stn1 were found to be involved in the regulation of telomere length, mutations in STN1 or CDC13 conferring an increase in telomere size. Loss of Stn1 function activated the RAD9 and MEC3 G2/M checkpoints, therefore confirming that DNA damage is generated. We propose that Stn1 functions in telomere metabolism during late S phase in cooperation with Cdc13 ...
New Sequences ============= S82971 S82971 1775bp DNA PLN 10-FEB-1997 PEX13=PAS20 [Saccharomyces cerevisiae, Genomic, 1775 nt]. PEX13; Pex13p. SCRGA1 X90950 4305bp DNA PLN 07-FEB-1997 S.cerevisiae rga1 (dbm1) gene. DBM1; pheromone response; RGA1 gene; RGA1 (DBM1); Rga1p (Dbm1p). SCU17262 U17262 3051bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip1p (PIP1) gene, complete cds. PIP1; Pip1p. SCU17263 U17263 2251bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip2p (PIP2) gene, complete cds. PIP2; Pip2p. SCU17264 U17264 1842bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae Pip3p (PIP3) gene, complete cds. PIP3; Pip3p. SCU85960 U85960 1720bp DNA PLN 11-FEB-1997 Saccharomyces cerevisiae RNA polymerase II-specific TBP associated factor Taf40p (TAF40) gene, complete cds. TAF40; RNA polymerase II specific TBP associated; factor. SCU86641 U86641 1657bp DNA PLN 08-FEB-1997 Saccharomyces cerevisiae Rim9p (RIM9) gene, complete cds. RIM9; Rim9p. =========== Updated Features/Annotations ============= YSCDYS1 ...
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(11)2-Cys6 binuclear cluster domin and a putative alpha2-SCB-alpha2 binding site ...
TY - JOUR. T1 - Molecular cloning and characterization of the RAD1 gene of Saccharomyces cerevisiae. AU - Higgins, David R.. AU - Prakash, Satya. AU - Reynolds, Paul. AU - Prakash, Louise. PY - 1983. Y1 - 1983. N2 - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or spores, or on sporulation.. AB - We have cloned the RAD1 gene of Saccharomyces cerevisiae and physically mapped it to a 4.0-kb DNA fragment from chromosome XVI. The RAD1 gene determines a transcript of 3.1 kb, and the direction of transcription was found to be leftwards, from EcoRI towards BglII (Fig. 1). Deletions of the RAD1 gene were made and were found to have no effect on viability of vegetative cells or ...
SPT16 was previously identified as a high-copy-number suppressor of delta insertion mutations in the 5 regions of the HIS4 and LYS2 genes of Saccharomyces cerevisiae. We have constructed null mutations in the SPT16 gene and have demonstrated that it is essential for growth. Temperature-sensitive-lethality spt16 alleles have been isolated and shown to be pleiotropic; at a temperature permissive for growth, spt16 mutations suppress delta insertion mutations, a deletion of the SUC2 upstream activating sequence, and mutations in trans-acting genes required for both SUC2 and Ty expression. In addition, SPT16 is identical to CDC68, a gene previously shown to be required for passage through the cell cycle control point START. However, at least some transcriptional effects caused by spt16 mutations are independent of arrest at START. These results and those in the accompanying paper (A. Rowley, R. A. Singer, and G. C. Johnston, Mol. Cell. Biol. 11:5718-5726, 1991) indicate that SPT16/CDC68 is required ...
TY - JOUR. T1 - A DNA integrity network in the yeast Saccharomyces cerevisiae. AU - Pan, Xuewen. AU - Ye, Ping. AU - Yuan, Daniel S.. AU - Wang, Xiaoling. AU - Bader, Joel S.. AU - Boeke, Jef D.. N1 - Funding Information: We thank members of the Boeke lab for valuable discussions and Pamela Meluh for critical comments on the manuscript. We thank Brian Peyser and Forrest Spencer for valuable discussions on synthetic lethality networks, Heng Zhu for the GAL1pr-GST-CTF4 and GAL1pr-GST overexpression plasmids, Alain Verreault for the GAL1pr-HHT plasmid, and Ivana Celic for sharing unpublished data. Raw data were submitted to GEO (Accession #GSE3574). We regret inability to cite many relevant studies of DNA metabolism and genomic instability due to space limits. Under a licensing agreement between Open Biosystems, Inc. and the Johns Hopkins University, the University is entitled to a share of royalties on sales of yeast strains described in this article. The terms of this arrangement are being ...
Evolution of multigene families are considered in the review on the example of the PHO gene family encoding the structure of acid phosphatases in the yeast Saccharomyces cerevisiae. Analysis of the...
TY - THES. T1 - Lipid transport to the plasma membrane of the yeast Saccharomyces cerevisiae. AU - Pichler, Harald. PY - 2000. Y1 - 2000. M3 - Doctoral Thesis. ER - ...
Saccharomyces cerevisiae Protein STE5 (STE5) ,partial datasheet and description hight quality product and Backed by our Guarantee
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TY - JOUR. T1 - Increased stress parameter synthesis in the yeast Saccharomyces cerevisiae after treatment with 4-hydroxy-2-nonenal. AU - Wonisch, Willibald. AU - Hayn, Marianne. AU - Schaur, Jörg. AU - Tatzber, Franz. AU - Kranner, Ilse. AU - Grill, Dieter. AU - Winkler, Rudolf. AU - Bilinski, Tomasz. AU - Kohlwein, Sepp-Dieter. AU - Esterbauer, Hermann. PY - 1997. Y1 - 1997. U2 - 10.1016/S0014-5793(97)00123-3. DO - 10.1016/S0014-5793(97)00123-3. M3 - Article. VL - 405. SP - 11. EP - 15. JO - FEBS letters. JF - FEBS letters. SN - 0014-5793. IS - 1. ER - ...
This unit presents detailed protocols for a range of centrifugation‐based subcellular fractionation procedures for the yeast Saccharomyces cerevisiae
Yeast Saccharomyces cerevisiae in vivo Prp8 splicing assay(A) Schematic representation of the two-step splicing pathway (SS, splice site; BS, branch site). Brie
MOTIZUKI, M., MITSUI, K., ENDO, Y. and TSURUGI, K. (1986), Detection and partial characterization of the chromatin-associated proteases of yeast Saccharomyces cerevisiae. European Journal of Biochemistry, 158: 345-350. doi: 10.1111/j.1432-1033.1986.tb09757.x ...
Budding Yeast: Saccharomyces cerevisiae Saccharomyces cerevisiae, the budding yeast, is the common yeast used in baking (bakers yeast) and brewing (brewers
TY - CHAP. T1 - Lipids and membranes in Saccharomyces cerevisiae.. AU - Schweizer, Michael. PY - 1999. Y1 - 1999. M3 - Chapter. SP - 79. EP - 155. BT - In The Metabolism & Molecular Physiology of Saccharomyces cerevisiae. Eds. J R Dickinson & M Schweizer. Taylor & Francis, London. ER - ...
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Saccharomyces cerevisiae ATCC ® 9763D-5™ Designation: Genomic DNA from Saccharomyces cerevisiae NRRL Y-567 (ATCC ® 9763™) Application: Food testing
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1P-022 Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測(遺伝子工学,一般講演)1P-022 Saccharomyces cerevisiaeの糖代謝における転写制御ネットワークの予測(遺伝子工学,一般講演)AN10549378 ...
The Saccharomyces Cerevisiae Morphological Database(SCMD) is a collection of micrographs of budding yeast mutants. Micorgraphs of mutants with altered cell morphology were taken at Ohya Group, University of Tokyo, from a set of the haploid MATa deleted strains obtained from EUROSCARF. From the micrographs, disruptant cells are automatically extracted by our novel cell-image processing software developed at Morishita Group, University of Tokyo. Heterozygous essential gene deletion set, DAmP collection set, natural yeast strain set and others were analyzed by this software. ...
The Saccharomyces Genome Database (SGD) provides comprehensive integrated biological information for the budding yeast Saccharomyces cerevisiae.
TY - JOUR. T1 - Degradation of HMG-CoA reductase-induced membranes in the fission yeast, Schizosaccharomyces pombe. AU - Lum, Pek Yee. AU - Wright, Robin. PY - 1995/10/1. Y1 - 1995/10/1. N2 - Elevated levels of certain membrane proteins, including the sterol biosynthetic enzyme HMG-CoA reductase, induce proliferation of the endoplasmic reticulum. When the amounts of these proteins return to basal levels, the proliferated membranes are degraded, but the molecular details of this degradation remain unknown. We have examined the degradation of HMG-CoA reductase-induced membranes in the fission yeast, Schizosaccharomyces pombe. In this yeast, increased levels of the Saccharomyces cerevisiae HMG-CoA reductase isozyme encoded by HMG1 induced several types of membranes, including karmellae, which formed a cap of stacked membranes that partially surrounded the nucleus. When expression of HMG1 was repressed, the karmellae detached from the nucleus and formed concentric, multilayered membrane whorls that ...
ERK5 is a mitogen-activated protein (MAP) kinase regulated in human cells by diverse mitogens and stresses but also suspected of mediating the effects of a number of oncogenes. Its expression in the slt2Delta Saccharomyces cerevisiae mutant rescued several of the phenotypes caused by the lack of Slt2p (Mpk1p) cell integrity MAP kinase. ERK5 is able to provide this cell integrity MAP kinase function in yeast, as it is activated by the cell integrity signaling cascade that normally activates Slt2p and, in its active form, able to stimulate at least one key Slt2p target (Rlm1p, the major transcriptional regulator of cell wall genes). In vitro ERK5 kinase activity was abolished by Hsp90 inhibition. ERK5 activity in vivo was also lost in a strain that expresses a mutant Hsp90 chaperone. Therefore, human ERK5 expressed in yeast is an Hsp90 client, despite the widely held belief that the protein kinases of the MAP kinase class are non-Hsp90-dependent activities. Two-hybrid and protein binding studies ...
Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is not yet completely understood. The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up on these studies, many homologs have been found in higher eukaryotes. Yeast remains a highly relevant model organism for studying autophagy, with a wide range of established methods to elucidate the molecular details of the autophagy pathway. In this review, we provide an overview of methods to study both selective and bulk autophagy, including intermediate steps in the yeast Saccharomyces cerevisiae. We compare different assays, discuss their advantages and
Saccharomyces cerevisiae is a species of budding yeast. It is perhaps the most useful yeast owing to its use since ancient times in baking and brewing. It is believed that it was originally isolated from the skins of grapes (one can see the yeast as a component of the thin white film on the skins of some dark-colored fruits such as plums; it exists among the waxes of the cuticle). It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology, much like Escherichia coli as the model prokaryote. It is the microorganism behind the most common type of fermentation. Saccharomyces cerevisiae cells are round to ovoid, 5-10 micrometres in diameter. It reproduces by a division process known as budding. It is useful in studying the cell cycle because it is easy to culture, but, as a eukaryote, it shares the complex internal cell structure of plants and animals. S. cerevisiae was the first eukaryotic genome that was completely sequenced. The yeast genome database [1] is ...
Saccharomyces Cerevisiae Yeast Cells Sem Scanning as a 8x6 Glass Mount from CMSP Photo Prints. Fast and safe delivery. Saccharomyces Cerevisiae Yeast Cells. these Microorganisms Fungi are Used to Raise Bread Dough the Yeasts Produce
Read The Genetic Control of Cell Growth and Development in Yeast Saccharomyces cerevisiae: Disturbed Sporulation in Diploids with a Decreased Activity of the Ras/cAMP Signal Transduction Pathway, Russian Journal of Genetics on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Getting Better Intestinal Health through the Addition of Yeast (Saccharomyces Cerevisiae) Combined with Threonine in Broilers Diets
Algerghina, L.; Porro, D.; Martegani, E.; Ranzi, B.M., 1991: Ethanol and biomass production from whey lactose by an engineered Saccharomyces cerevisiae strain
Ubiquitin carrier proteins (E2s) are involved in the covalent attachment of ubiquitin to a variety of cellular target proteins in eukaryotes. Here, we report the cloning of genes from wheat and Arabidopsis thaliana that encode 16-kDa E2s and a domain analysis of E2s by in vitro mutagenesis. The genes for E216kDa, which we have designated wheat and At UBC1, encode proteins that are only 33% identical (58% similar) with a 23-kDa E2 from wheat (encoded by the gene now designated wheat UBC4), but are 63% identical (82% similar) with the E2 encoded by the Saccharomyces cerevisiae DNA repair gene, RAD6. Unlike the proteins encoded by RAD6 and wheat UBC4, the UBC1 gene products lack acidic C-terminal domains extending beyond the conserved core of the proteins and are incapable of efficient in vitro ligation of ubiquitin to histones. From enzymatic analysis of the UBC1 and UBC4 gene products mutagenized in vitro, we have identified several domains important for E2 function, including the active site ...
I use this paper in my graduate genetics course. It describes a global screen for synthetic defects involving DNA integrity, which reveals a network of 16 functional modules. The paper illustrates screens based on genetic interactions (in this case, synthetic lethality or fitness defects) and the systems biology used to evaluate the results of such a screen. It also illustrates the use of Saccharomyces cerevisiae as a model system ...
Mitochondrial matrix space Mg2+ is important for many aspects of nucleotide metabolism [37, 38]. Two inner mitochondrial membrane transporters, Mrs2p and Lpe10p, are needed for group II intron splicing [16, 39]. MRS2 and LPE10 have slight sequence similarity with the bacterial Mg2+transporter CorA. Assays with a fluorescent Mg2+ indicator dye indicate that Mrs2p is part of an electrophoretic mitochondrial Mg2+ influx pathway inhibited by cobalt(III)hexaammine [30]. Mitochondrial Mg2+ levels changed with the levels of Mrs2p and Lpe10p. Mitochondrial electrophoretic Mg2+ uptake was absent in an MRS2 deletion strain. Mrs2p and Lpe10p are essential for yeast growth on nonfermentable carbon sources [38]. However they cannot substitute for each other suggesting non-redundant functions. It is possible that Mrs2p or Lpe10p is responsible for the mitochondrial Mg2+ release described in this report. However, in the previous experiments Mg2+ was taken up by energized mitochondria in an Mrs2p-dependent ...
Yeast strains and cell culture: Schizosaccharomyces pombe strains used were a haploid strain (h− leu1-32 ura4-D18 ade6-m216), a diploid strain (h−/h+ leu1-32/leu1-32 ura4-D18/ura4-D18 ade6-m210/ade6-m216), and a mutant haploid strain pim1-d1ts (h− leu1-32 ura4-D18 pim1-d1ts; Sazer and Nurse 1994), all of which are derived from strain 972 (Leupold 1970). Cell culture conditions, media composition, and genetic analyses have been described previously (Morenoet al. 1991).. nmt1 promoter regulation: Gene expression under the control of the nmt1 promoter (Maundrell 1990) in pREP3X or pREP41X (Forsburg 1993) was repressed by the inclusion of 5 μg/ml thiamine in the Edinburgh Minimal Media (EMM; Morenoet al. 1991). To derepress expression, cells were washed three times with thiamine-free EMM and grown in fresh thiamine-free EMM.. cDNA library screen and DNA manipulations: An S. pombe cDNA library (a gift from Bruce Edgar and Chris Norbury) in the pREP3X vector (Forsburg 1993) was transformed into ...
The biological interpretation of genetic interactions is a major challenge. Recently, Kelley and Ideker proposed a method to analyze together genetic and physical networks, which explains many of the known genetic interactions as linking different pathways in the physical network. Here, we extend this method and devise novel analytic tools for interpreting genetic interactions in a physical context. Applying these tools on a large-scale Saccharomyces cerevisiae data set, our analysis reveals 140 between-pathway models that explain 3765 genetic interactions, roughly doubling those that were previously explained. Model genes tend to have short mRNA half-lives and many phosphorylation sites, suggesting that their stringent regulation is linked to pathway redundancy. We also identify pivot proteins that have many physical interactions with both pathways in our models, and show that pivots tend to be essential and highly conserved. Our analysis of models and pivots sheds light on the organization of the
Yeast from Saccharomyces cerevisiae Type II; Synonym: (Bakers yeast); find Sigma-Aldrich-YSC2 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.
Saccharomyces cerevisiae, HA12, a, ade1 ade2. Our materials are for use in the experiments developed by the yeast genetics educational network (GENE project) created by Dr. Tom Manney at Kansas State University (KSU). These experiments are great for hands-on teaching of some of the basic concepts in...
This data-set represents a genome-scale metabolomic map about amino acid metabolism in gene deletion strains as determined in Saccharomyces cerevisiae.
The Saccharomyces Genome Database (SGD) provides comprehensive integrated biological information for the budding yeast Saccharomyces cerevisiae.
TY - CHAP. T1 - Axl1p gene product (Saccharomyces cerevisiae). AU - Schmidt, Walter K.. AU - Michaelis, Susan. PY - 2004/6/30. Y1 - 2004/6/30. UR - http://www.scopus.com/inward/record.url?scp=84944035697&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84944035697&partnerID=8YFLogxK. U2 - 10.1016/B978-0-12-079611-3.50269-X. DO - 10.1016/B978-0-12-079611-3.50269-X. M3 - Chapter. AN - SCOPUS:84944035697. SN - 9780120796113. VL - 1. SP - 879. EP - 882. BT - Aspartic and Metallo Peptidases. PB - Elsevier Inc.. ER - ...
Domain architecture and assignment details (superfamily, family, region, evalue) for YER138C from Saccharomyces cerevisiae SGD. Plus protein sequence and external database links.
Domain architecture and assignment details (superfamily, family, region, evalue) for YGL175C from Saccharomyces cerevisiae SGD. Plus protein sequence and external database links.
Domain architectures containing the following SCOP superfamilies _gap_,100950,_gap_ in Saccharomyces cerevisiae SGD. Domain architectures illustrate each occurrence of _gap_,100950,_gap_.
A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged MCM1. MCM1 is a transcription factor involved in cell-type-specific trans...
Saccharomyces cerevisiae is a species of yeast. It is believed to be isolated from the skin of grapes. It is one of the most intensively studied eukaryot..
Domain architecture and assignment details (superfamily, family, region, evalue) for YIL065C from Saccharomyces cerevisiae FL100. Plus protein sequence and external database links.
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cdc15, a protein kinase involves in cytokinesis. These phase and GFPimages ...
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cln2. Cln2 is a G1 cyclin involved in regulation of the cell cycle; activate...
A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Bck2, a Cln-independent activator of CLN1,2 expression. These phase and gfp...
Involved in the dephosphorylation of the large subunit of RNA polymerase II. Is required in late G1 for normal G1 cyclin expression, bud initiation and expression of certain genes that are periodically expressed during late G1. Associates with the SAP proteins in a cell cycle-dependent manner.
BioAssay record AID 460553 submitted by ChEMBL: Antiaging effect in Saccharomyces cerevisiae K6001 expressing uth1 mutant assessed as extension of replicative life span after 2 days.
Functional Overlap between eIF4G Isoforms in Saccharomyces cerevisiae. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Gene target information for GLR1 - glutathione-disulfide reductase GLR1 (Saccharomyces cerevisiae S288C). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Fungal Diversity. 65 (1): 77-88. doi:10.1007/s13225-013-0252-7. G. S. de Hoog, Guarro J; Gene J; Figueras MJ (2000). Atlas of ... When the fungal infection only concerns with systemic involvement except the brain, the probability of cure is higher. In ... Mazur, Joseph E. (1 February 2001). "A Case Report of a Dactylaria Fungal Infection in a Lung Transplant Patient. *. ". Chest. ... "Fungal Databases Nomenclature and Species Banks". International Mycological Association. BioloMICS Net. Retrieved 15 October ...
American geneticist One gene-one enzyme hypothesis - The idea that genes act through the production of enzymes, with each gene ... "The Neurospora Compendium". Fungal Genetics Stock Center (FGSC). Retrieved December 27, 2005. "The Neurospora-Fungal Genome ... This led them to propose the "one gene, one enzyme" hypothesis that specific genes code for specific proteins. Their hypothesis ... The number of genes affecting the diploid stage was estimated to be at least 435 (about 4% of the total number of 9,730 genes ...
"Application of Mating Type Gene Technology to Problems in Fungal Biology". Annual Review of Phytopathology. 36: 115-137. doi: ... The first mating pheromone-receptor genes characterized were for U. maydis. The A or b mating locus contains genes that code ... The results of these experiments led directly to the "one gene, one enzyme" hypothesis that specific genes code for specific ... The number of genes affecting the diploid stage was estimated to be at least 435 (about 4% of the total number of 9,730 genes ...
showed that the genes required for meiosis are present in T. marneffei, and that mating and genetic recombination occur in this ... O'Gorman CM, Fuller H, Dyer PS (January 2009). "Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus ... Sexuality generates diversity in the aflatoxin gene cluster in A. flavus, suggesting that production of genetic variation may ... Fincham J RS, Day PR (1963). Fungal Genetics. Oxford, UK: Blackwell Scientific Publications. ASIN B000W851KO. Wagner RP, ...
Ghabrial SA (1998). "Origin, adaptation and evolutionary pathways of fungal viruses". Virus Genes. 16 (1): 119-31. doi:10.1023/ ... Some genes of RNA virus are important to the viral replication cycles and mutations are not tolerated. For example, the region ... The majority of fungal viruses are double-stranded RNA viruses. A small number of positive-strand RNA viruses have been ... After this DNA is integrated into the host genome using the viral enzyme integrase, expression of the encoded genes may lead to ...
1992). "Fungal mating type genes - regulators of sexual development". Mycological Research. 96 (12): 993-1006. doi:10.1016/ ... Using elegant genetic and molecular techniques with different genetic stocks including gene deletions and gene fusions, she has ... A factors contain several duplicate genes coding for the A protein but it is only necessary for any one of these genes to be ... She was Professor Emeritus of Fungal Genetics in the Department of Plant Science at the University of Oxford, and was known for ...
If the mutated base pair is in the promoter sequence of a gene, then the expression of the gene may change. Also, if the ... Because RIP is so efficient at detecting and mutating repeats, fungal biologists often use it as a tool for mutagenesis. A ... This genetic defect is located in the HEXA gene, which is found on chromosome 15. The HEXA gene makes part of an enzyme called ... These regions are either non-coding regions or genes encoding small secreted proteins including avirulence genes. The degree of ...
"Decoding fungal genome: Salute to Dr Maqsudul Alam". Dhaka Courier. Archived from the original on 28 September 2014. Retrieved ... "Bangladeshi scientists invent fungus gene". New Age. 20 September 2012. Archived from the original on 13 November 2012. ... "BD scientists crack genome sequencing of fungal pathogen". The Financial Express. Dhaka. Retrieved 7 July 2013. " ...
Turgeon, B.G.; Yoder, O.C. (2000). "Proposed Nomenclature for Mating Type Genes of Filamentous Ascomycetes". Fungal Genetics ... Mating type genes in ascomycetes are called idiomorphs rather than alleles due to the uncertainty of the origin by common ... Outcrossing, through complementation, could provide the benefit of masking recessive deleterious mutations in genes that ...
... gene rodA), these proteins are the main component of the hydrophobic sheath covering the surface of many fungal spores. Genomic ... a new Aspergillus developmental mutant induced by directed gene inactivation". Genes & Development. 5 (7): 1161-71. doi:10.1101 ... Nakari-Setälä T, Azeredo J, Henriques M, Oliveira R, Teixeira J, Linder M, Penttilä M (July 2002). "Expression of a fungal ... Wessels J, De Vries O, Asgeirsdottir SA, Schuren F (August 1991). "Hydrophobin Genes Involved in Formation of Aerial Hyphae and ...
Janson, Sven; Graneli, Edna (September 2003). "Genetic analysis of the psbA gene from single cells indicates a cryptomonad ... lichens are named and classified as fungal species. The association is termed a morphogenesis because the lichen has a form and ... The photobiont possibly triggers otherwise latent genes in the mycobiont. Trentepohlia is an example of a common green alga ... genes from Helicosporidium spp.: Evidence supporting the reclassification of Helicosporidia as green algae (Chlorophyta)". ...
"Comparison of loline alkaloid gene clusters across fungal endophytes: predicting the co-regulatory sequence motifs and the ... of selected LOL genes by RNA interference and gene knockout have directly confirmed the involvement of two of the LOL genes in ... of at least eleven genes. The LOL genes are greatly and coordinately upregulated during loline alkaloid production, and ... These tests and similarities in the peptide sequences of the proteins encoded by these genes to known enzymes indicate that one ...
Taylor, D. J.; J. Bruenn (2009). "The evolution of novel fungal genes from non-retroviral RNA viruses". BMC Biology. 7: 88. doi ... Koonin, E. (2010). "Taming of the shrewd: novel eukaryotic genes from RNA viruses". BMC Biology. 8: 2. doi:10.1186/1741-7007-8- ...
Lenormand T (2002). "Gene flow and the limits to natural selection". Trends in Ecology and Evolution. 17 (4): 183-189. doi: ... 10.1016/S0169-5347(02)02497-7. Ingold CT (1971). Fungal spores: their liberation and dispersal. Oxford: Clarendon Press. pp. ... Technically, dispersal is defined as any movement that has the potential to lead to gene flow. The act of dispersal involves ... The formation of barriers to dispersal or gene flow between adjacent areas can isolate populations on either side of the ...
"Positive identification of a lambda gt11 clone containing a region of fungal phytase gene by immunoprobe and sequence ... "Transgenic maize plants expressing a fungal phytase gene". Transgenic Res. 17 (4): 633-643. doi:10.1007/s11248-007-9138-3. PMID ... characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger". Gene. 127 (1): 87-94. doi: ... Aspergillus (ficuum) niger fungal strain NRRL 3135 (ATCC 66876) was identified as a promising candidate as it was able to ...
Torres-Garcia, Sito (2020). "Epigenetic gene silencing by heterochromatin primes fungal resistance". Nature. 585 (7825): 453- ... he discovered that genes are silenced when placed within fission yeast centromeres and telomeres, and then utilised this gene ... Ard, Ryan; Tong, Pin; Allshire, Robin C (2014). "Long non-coding RNA-mediated transcriptional interference of a permease gene ... Ard, Ryan; Allshire, Robin C (2016). "Transcription-coupled changes to chromatin underpin gene silencing by transcriptional ...
"Comparison of loline alkaloid gene clusters across fungal endophytes: predicting the co-regulatory sequence motifs and the ... of selected LOL genes by RNA interference and gene knockout have directly confirmed the involvement of two of the LOL genes in ... of at least eleven genes.[20][26] The LOL genes are greatly and coordinately upregulated during loline alkaloid production,[24] ... The lolines are insecticidal and insect-deterrent compounds that are produced in grasses infected by endophytic fungal ...
Next-generation sequencing technologies generate large amounts of data, and analysis of fungal marker-gene data is an active ... fungal diversity in forest soils,[6] stratification of fungal communities in soil horizons,[7] and fungal succession on ... Fungal diversity[edit]. Exploration of fungal diversity in situ has also benefited from next-generation DNA sequencing ... marker genes) to identify and describe the distribution of taxonomic groups in the fungal community in question, though more ...
1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to ... The ribosomal DNA contains one transcriptional unit with a cluster of genes coding for the 18S, 5.8S and 28S rRNAs and two ... DNA of plant pathogenic mycoplasma-like organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene ... relationships because they exhibit greater interspecies differences than the smaller and larger subunits of rRNA genes. The use ...
Guarro, J; Gams, W; Puhhol, I; Gene, J (1997). "Acremonium species: new emerging fungal opportunists-in vitro antifungal ... Freezing the fungal BMOs at -80 °C for 4 weeks did not affect the Mn(II) ability, and the reducible Mn was still dominated in ... This makes fungal BMOs an effective Mn(II) sequestering material if needed. For example, it can be used for the continuous ... Analysis of the genes for ribosomal large subunit (LSU) and whole small subunit (SSU) also help to elucidate phylogenetic ...
Adams, M; Antoniw, J (January 2006). "DPVweb: a comprehensive database of plant and fungal virus genes and genomes". Nucleic ... For each curated sequence the database contains the start and end positions of each feature (gene, non-translated region, etc ... As far as possible, the nomenclature for genes and proteins are standardized within genera and families. Sequences of features ... fungi and protozoa that are complete or that contain at least one complete gene. There are currently about 10,000 such ...
Many of the genes involved in such processes have been shown to impact virulence through experiments involving genetic mutation ... The transcription factor SrbA is the master regulator in the fungal response to hypoxia in vivo and is essential in many ... Targeted mutation of the ftrA gene did not induce a decrease in virulence in the murine model of A. fumigatus invasion. In ... Targeted mutation of the afareA gene showed a decrease in onset of mortality in a mouse model of invasion. The Ras regulated ...
2009). "Ochratoxin A induces apoptosis in neuronal cells". Genes Nutr. 4 (1): 41-48. doi:10.1007/s12263-008-0109-y. PMC 2654052 ... 2006). "Acute neurotoxic effects of the fungal metabolite ochratoxin A". Neurotoxicology. 27 (1): 82-92. doi:10.1016/j.neuro. ... Genes & Diseases. 2 (3): 247-254. doi:10.1016/j.gendis.2015.02.008. PMC 6150079. PMID 30258868.. ...
"The evolution of novel fungal genes from non-retroviral RNA viruses". BMC Biology. 7: 88. doi:10.1186/1741-7007-7-88. Koonin, E ... Distinct, syncytin-like genes have been identified in primates, rodents, lagomorphs, carnivores, and ungulates, with ... Remarkably, the capture of syncytin or syncytin-like genes has occurred independently, from different groups of endogenous ... Dupressoir, A.; Lavialle, C.; Heidmann, T. (2012). "From ancestral infectious retroviruses to bona fide cellular genes: role of ...
"Cloning and sequence analysis of three Gα subunit genes from Colletotrichum hanaui." Acta Agriculturae Zhejiangensis 6 (2010): ... "Anthracnose disease of switchgrass caused by the novel fungal species Colletotrichum navitas." Mycological research 113.12 ( ...
These genes, termed InvA1,2,3,4,5 and 6, were overexpressed in E. coli and the genes were characterized by co-incubating the ... Fungal Genetics and Biology. 45 (11): 1487-1496. doi:10.1016/j.fgb.2008.08.009. PMID 18805498. Atromentin synthetase on www. ... The genes for the atromentin synthetase and aminotransferase from S. lacrymans were up-regulated during co-incubation with ... The genetic basis of these two genes is clustered (i.e., adjacent to one another). These enzymes were first characterized in ...
Altincicek, B.; Kovacs, J.L.; Gerardo, N.M. (2012). "Horizontally transferred fungal carotenoid genes in the two-spotted spider ... Buchnera aphidicola gene transcription, although not well understood, is thought to be regulated by a small number of global ... Five of the bacteria genes have been transferred to the aphid nucleus. The original association may is estimated to have ... Despite the apparent loss of transcription factors in the reduced genome, gene expression is highly regulated, as shown by the ...
Stringer MA, Timberlake WE (February 1993). "Cerato-ulmin, a toxin involved in Dutch elm disease, is a fungal hydrophobin". The ... Virus Genes. 33 (1): 33-9. doi:10.1007/s11262-005-0028-6. PMID 16791416. S2CID 7774135. ...
Boran Altincicek; Jennifer L. Kovacs; Nicole M. Gerardo (2011). "Horizontally transferred fungal carotenoid genes in the two- ... with the exception of some aphids and spider mites which acquired the synthesizing genes from fungi). Carotenes are ...
Fungal Prions (Pilz-Prionen). Vorgeschlagene oder nicht-offizielle Virustaxa[Bearbeiten , Quelltext bearbeiten]. Die Virus- ... Familie Geminiviridae (ssDNA(+/-): die einzelnen Gene haben unterschiedliche Polarität). *Genus Becurtovirus. *Genus ...
The study has uncovered toxin genes and other genes that may be responsible for the virulence of the fungus. In the long term ... Hymenoscyphus fraxineus is an Ascomycete fungus that causes ash dieback, a chronic fungal disease of ash trees in Europe ... researchers aim to find the genes that confer resistance to the pathogen on some ash trees.[12] ... Fungal Ecology. 5 (2): 147-153. doi:10.1016/j.funeco.2011.10.004.. ...
Two genes, LAC12 and LAC4, allow K. marxianus to absorb and use lactose as a carbon source.[5] This species is considered to be ... Fungal Biology Reviews. 24 (1-2): 17-26. doi:10.1016/j.fbr.2010.01.001.. ... Using 18S rRNA gene sequencing, it was suggested that K. marxianus, K. aestuarii, K. dobzhanskii, K. lactic, K. wickerhamii, K ... marxianus possesses the necessary genes to be crabtree positive.[5] K. marxianus is highly thermotolerant and able to withstand ...
Tertiary gene pool: L. lamottei and L. tomentosus. Crosses generally fail between members of different gene pools. However, ... Fungal diseases[edit]. Fungal diseases. Alternaria blight Alternaria alternata. Alternaria sp. Anthracnose Colletotrichum ... According to their inter-crossability Lens species can be divided into three gene pools: *Primary gene pool: L. culinaris (and ... Even if crosses are successful, many undesired genes may be introduced as well in addition to the desired ones. This can be ...
The studies on fungal evolutionary genomics will further elucidate the earliest stages of divergence as a result of gene flow, ... Such a gene that exhibits multiple phenotypic expression is called a pleiotropic gene . Therefore mutation in a pleiotropic ... Gene pleiotropy occurs when a gene product interacts with multiple other proteins or catalyzes multiple reactions. ... Pleiotropy describes the genetic effect of a single gene on multiple phenotypic traits. The underlying mechanism is genes that ...
In late autumn, chemicals on the skin of the caterpillar interact with the fungal spores and release the fungal mycelia, which ... 2007). "A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a ... Gradually the host larvae become rigid because of the production of fungal sclerotia. Fungal sclerotia are multihyphal ... O. sinensis consists of two parts, a fungal endosclerotium (within the caterpillar) and stroma.[10] The stroma is the upper ...
Yeast and fungal β-glucans contain 1-6 side branches, while cereal β-glucans contain both β-1,3 and β-1,4 backbone bonds. The ... The process leading to β-1,6 linkages is poorly understood: although genes important in the process have been identified, not ... Cereal and fungal products have been used for centuries for medicinal and cosmetic purposes; however, the specific role of β- ... 1995). "Plasma (13)-beta-D-glucan measurement in diagnosis of invasive deep mycosis and fungal febrile episodes". Lancet. 345 ( ...
More research on this fungal species, Cunninghamella japonica, and others, is likely to appear in the near future.[112] The ... Gene therapy. *Head transplant. *Isolated brain. *Life extension *Strategies for Engineered Negligible Senescence ...
Zhao, ZJ; Tong, HR; Zhou, L; Wang, EX; Liu, QJ (2010). "FUNGAL COLONIZATION OF PU‐ERH TEA IN YUNNAN". Journal of Food Safety. ... It is apparent that this species does not have the gene sequence for coding ochratoxin and thus pu'er tea should be considered ... The bacterial and fungal cultures found in the fermenting piles were found to vary widely from factory to factory throughout ... Aged raw pu'erh: Aged pu'er should never smell moldy, musty, or strongly fungal, though some pu'erh drinkers consider these ...
Genes[edit]. PEX genes encode the protein machinery ("peroxins") required for proper peroxisome assembly, as described above. ... The peroxisome of plant cells is polarised when fighting fungal penetration. Infection causes a glucosinolate molecule to play ... Genes that encode peroxin proteins include: PEX1, PEX2 (PXMP3), PEX3, PEX5, PEX6, PEX7, PEX10, PEX11A, PEX11B, PEX11G, PEX12, ... doi:10.1016/j.gene.2009.09.014. PMID 19818387.. *^ Gabaldón T, Capella-Gutiérrez S (Oct 2010). "Lack of phylogenetic support ...
Fungal Biology 118(1) 32-47. Qi, Y. X., et al. (2007). Detection of Corynespora cassiicola in Hevea rubber tree from China. ... 2014). Diversity of the cassiicolin gene in Corynespora cassiicola and relation with the pathogenicity in Hevea brasiliensis. ... USDA ARS Fungal Database. Dixon, L. J., et al. (2009). Host specialization and phylogenetic diversity of Corynespora cassiicola ... the leaf wetness is likely a major environmental factor driving the disease for this fungal pathogen. The humidity may be used ...
"Genes and chronic obstructive pulmonary disease". The Medical Clinics of North America. 96 (4): 699-711. doi:10.1016/j.mcna. ...
Sequences of the matK chloroplast gene,[18] as well as sequences of chloroplast and nuclear genomes,[19] reveal close ... Laurel wilt disease, caused by the virulent fungal pathogen Raffaelea lauricola, a native of southern Asia, was found in the ... Backing has come from matK sequences of chloroplast genes[18] while a questionable placement of Cassytha has been concluded ... Partial support for the tribe is also attained from the matK sequences of chloroplast genes[18] as well as embryology.[22] ...
... viable daughter cells to be produced would require an extra replication of the intact RNA gene homologous to any RNA gene that ... If at least one damage-free copy of each RNA gene is present in the transient diploid, viable progeny can be formed. For two, ... Tucker BJ, Breaker RR (Jun 2005). "Riboswitches as versatile gene control elements". Current Opinion in Structural Biology. 15 ... Lamb RA, Choppin PW (1983). "The gene structure and replication of influenza virus". Annual Review of Biochemistry. 52: 467-506 ...
... and UBIAD1 genes. Mutations in TGFBI which encodes transforming growth factor beta induced cause several forms of corneal ... caused by steroid sulfatase gene mutations and are currently usually not included under the rubric of the corneal dystrophies. ...
65,0 65,1 65,2 «Asthma genetics 2006: the long and winding road to gene discovery»։ Genes Immun 7 (2): 95-100։ 2006։ PMID ... Sharpe RA, Bearman N, Thornton CR, Husk K, Osborne NJ (January 2015)։ «Indoor fungal diversity and asthma: a meta-analysis and ... 10,0 10,1 10,2 Martinez F. D. (2007)։ «Genes, environments, development and asthma: a reappraisal»։ European Respiratory ... 16395390։ doi:10.1038/sj.gene.6364284 ,vauthors=. պարամետրը գոյություն չունի (օգնություն) *↑ Halapi E, Bjornsdottir, US ( ...
... is executed by autophagy-related (Atg) genes. The first autophagy genes were identified by genetic screens conducted ... In fungal cells on the other hand microplipophagy constitutes the main pathway and is especially well studied in yeast ... and that at least 15 APG genes are involved in autophagy in yeast.[69] A gene known as ATG7 has been implicated in nutrient- ... When the Beclin1 gene was altered to be heterozygous (Beclin 1+/-), the mice were found to be tumor prone.[90] However, when ...
Normally, the presence of a specific gene or an allele of a gene confers upon the cell the ability to grow in the selective ... Fungal media. *Czapek medium. *Dermatophyte test medium. *Potato dextrose agar. *Sabouraud agar ... In such cases, the gene is termed a marker. Selective growth media for eukaryotic cells commonly contain neomycin to select ... cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. Gancyclovir is ...
A clp gene homologue belonging to the crp gene family globally regulates lytic enzyme production, antimicrobial activity, and ... Characterisation of Lysobacter enzymogenes (Christensen and Cook 1978) strain 3.1T8, a powerful antagonist of fungal diseases ... making mutation effects of non-related genes difficult or nearly impossible to evaluate. However, strains harboring clp gene ... including mutants affected in structural genes encoding enzyme activities, the regulatory clp gene and various combinations ...
Replication between viruses is greatly varied and depends on the type of genes involved in them. Most DNA viruses assemble in ... Bacterial infections are treated with antibacterials (often called antibiotics) whereas fungal and viral infections are treated ...
... gene therapy, monoclonal antibodies and cell therapy (for instance, stem-cell therapies). Other ways to classify medicines are ... Anti-fungal: imidazoles, polyenes. *Anti-inflammatory: NSAIDs, corticosteroids. *Anti-allergy: mast cell inhibitors ... gene therapy, and cell therapy (for instance, stem cell therapies). ...
... the voltage-gated potassium channel KCNH2 gene (hERG/Kv11.1), and the α1D-adrenoceptor ADRA1D gene.[58] ... Itraconazole and ketoconazole, both used to treat fungal infections, are potent CYP3A4 inhibitors and increase the plasma ... such as polymorphisms in the drug transporter gene ABCB1 (which encodes P-glycoprotein), ...
Morris KL (2008). "Epigenetic Regulation of Gene Expression". RNA and the Regulation of Gene Expression: A Hidden Layer of ... For more details on this topic, see Fungal prions.. Prions are infectious forms of proteins. In general, proteins fold into ... They control gene expression including virulence genes in pathogens and are viewed as new targets in the fight against drug- ... There are several layers of regulation of gene expression. One way that genes are regulated is through the remodeling of ...
As with all long-lived plant and fungal species, no individual part of a clonal colony is alive (in the sense of active ...
... as well as two genes found previously only in birds, amphibians, and fish. More than 80% of the platypus's genes are common to ... "Platypus Fungal Disease". Department of Primary Industries and Water, Tasmania. 29 August 2008. Archived from the original on 7 ... The female platypus has a pair of ovaries, but only the left one is functional.[50] The platypus's genes are a possible ... a study found that the mechanism of sex determination is the AMH gene on the oldest Y chromosome.[79][80] A draft version of ...
Duquesne S, Destoumieux-Garzón D, Peduzzi J, Rebuffat S; Destoumieux-Garzón; Peduzzi; Rebuffat (August 2007). "Microcins, gene- ... fungal peptides, invertebrate peptides, amphibian/skin peptides, venom peptides, cancer/anticancer peptides, vaccine peptides ... gene) hexarelin. There is ongoing controversy on the legality of using secretagogue peptides in sports.[25] ...
Deng, M.; Lancto, C. A.; Abrahamsen, M. S. (2004). "Cryptosporidium parvum regulation of human epithelial cell gene expression ... Sebghati TS, Engle JT, Goldman WE (November 2000). "Intracellular parasitism by Histoplasma capsulatum: fungal virulence and ...
Upregulation of target genes can also render the fungicide ineffective. This is seen in DMI-resistant strains of Venturia ... Interaction of 2,4,5-trich,orophenylsulphonylmethyl thiocyanate with fungal spores *^ Latijnhouwers M, de Wit PJ, Govers F. ... In qualitative or discrete resistance, a mutation (normally to a single gene) produces a race of a fungus with a high degree of ... The 14a-demethylase (CYP51A1) gene is overexpressed in V. inaequalis strains resistant to myclobutanil. Phytopathology 91:102- ...
Many species will spread out their wings and expose them to direct sunlight and this too is thought to help in reducing fungal ... For females, possible benefits of extra-pair copulation include getting better genes for her offspring and insuring against the ... "Parsimony and Model-Based Analyses of Indels in Avian Nuclear Genes Reveal Congruent and Incongruent Phylogenetic Signals" ...
"Social Bats Pay a Price: Fungal Disease, White-Nose Syndrome ... Extinction?". The National Science Foundation. 3 July 2012. ... gene from bats (Chiroptera)". Genetics Research. 91 (2): 101-9. doi:10.1017/s0016672309000032. PMID 19393126.. ... the gene controlling Bmp signalling, Bmp2, is subjected to increased expression in bat forelimbs-resulting in the extension of ... Analyses of the sequence of the vocalization gene FoxP2 were inconclusive on whether laryngeal echolocation was lost in the ...
... expression signatures in mice that are linked to Candida albicans bloodstream infections and can discern between these fungal ... research team has pinned down gene expression signatures in mice that coincide with bloodstream infections caused by the fungal ...
Individuals with certain gene variants appear less able to resist Candida skin infections, explaining the observation ... These genes were fully sequenced in patients and unaffected family members to highlight differences that could account for the ... Individuals with certain gene variants appear less able to resist Candida skin infections, explaining the observation that ... Dutch and British researchers found missense mutations in the STAT1 gene, an important player in immune function, according to ...
Candida albicans thus takes advantage of both clonality and gene flow to diversify. The fungal pathogen Candida albicans can ... Candida albicans is the second most frequent agent of human fungal infections worldwide, causing high-mortality rates. Here we ... analyse the genomic sequences of 182 C. albicans isolates collected worldwide and find evidence of gene flow and thus ... Although, C. albicans shows a predominantly clonal population structure, we find evidence of gene flow between previously known ...
... Howard S. Judelson judelson at UCRAC1.UCR.EDU Sun Mar 17 17 ... These include the positional cloning of genes determining mating type (sexual compatibility type; see Judelson et al. 1995, ... and fungal transformation; experience with these would be helpful but is not required. Please sent a curriculum vitae and the ... and the analysis of genes determining resistance to fungicides. Major funding for these positions comes from grants from the ...
Fungal Parasitism Marked by Gene Loss, Not Gain by Jeffrey P. Tomkins, Ph.D. * Evidence for Creation › Evidence from Science › ... Like the fungal pathogen in the first study, it is also a biotroph. In this second study, researchers found "292 genes present ... Gene Loss Rather Than Gene Gain Is Associated with a Host Jump from Monocots to Dicots in the Smut Fungus Melanopsichium ... "highlighting that gene loss rather than gene gain is the hallmark of adaptation after the host jump to the dicot host." ...
Regulation of Mating and Filamentation Genes by Two Distinct Ste12 Complexes in Saccharomyces cerevisiae Song Chou, Shelley ...
... both aphids and adelgids acquired carotenoid biosynthesis genes from a fungal donor that result in ecologically relevant ... and demonstrated that they share the common fungal origin as those of aphids and adelgids based on their exon-intron gene ... Here, we examined the transcriptomes of nine phylloxerid species, and performed PCR to amplify carotenoid genes from their ... Unlike aphids that duplicated these genes in their genomes, phylloxerids maintained only single copies, and some species may ...
Identification of Azole-Responsive Genes by Microarray Technology: Why Are We Missing the Efflux Transporter Genes? D. P. ... A Nonsense Mutation in the ERG6 Gene Leads to Reduced Susceptibility to Polyenes in a Clinical Isolate of Candida glabrata ... Clade-Specific Flucytosine Resistance Is Due to a Single Nucleotide Change in the FUR1 Gene of Candida albicans Andrew R. ... Emergence of Echinocandin Resistance Due to a Point Mutation in the fks1 Gene of Aspergillus fumigatus in a Patient with ...
Controlled Expression of the Dominant Flocculation Genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae Patrick Govender, ... Shuffling of Promoters for Multiple Genes To Optimize Xylose Fermentation in an Engineered Saccharomyces cerevisiae Strain ... Saline-Dependent Regulation of Manganese Peroxidase Genes in the Hypersaline-Tolerant White Rot Fungus Phlebia sp. Strain MG-60 ... Differential Regulation and Posttranslational Processing of the Class II Hydrophobin Genes from the Biocontrol Fungus Hypocrea ...
Recently, recombinant DNA technology has enabled the bacteria to coexpress the specific genes. Genes encoding superior genes ... Xylanase coding gene (xynA-7) source. 16. Orpinomyces sp. GMLF5. Bifunctional cellulase and lichenase coding gene (celA-5) ... Studies on the functional transfer of rumen fungal genes to tractable bioprocessing hosts are rapidly emerging27. In this study ... To coexpress the xynA-7 gene of Neocallimastix sp. GMLF7 (800 bp fragment) and celA-5 gene of Orpinomyces sp. GMLF5 (1500 bp ...
We modified a gene knock-out procedure (Colot et al. 2006) to serve as a convenient knock-in strategy for Neurospora genes. We ... Extracts from strains with or without (−) the FLAG-tagged dim-2 gene and/or the GFP-tagged hpo gene at the his-3 (His) locus or ... Tools for Fungal Proteomics: Multifunctional Neurospora Vectors for Gene Replacement, Protein Expression and Protein ... Tools for Fungal Proteomics: Multifunctional Neurospora Vectors for Gene Replacement, Protein Expression and Protein ...
Title: Identification of Glutaminyl Cyclase Genes Involved in Pyroglutamate Modification of Fungal Lignocellulolytic Enzymes. ... Identification of Glutaminyl Cyclase Genes Involved in Pyroglutamate Modification of Fungal Lignocellulolytic Enzymes. United ... Identification of Glutaminyl Cyclase Genes Involved in Pyroglutamate Modification of Fungal Lignocellulolytic Enzymes. United ... "Identification of Glutaminyl Cyclase Genes Involved in Pyroglutamate Modification of Fungal Lignocellulolytic Enzymes". United ...
Fungal diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ... molecular methods have provided new insight into the richness of fungal communities present in both indoor and outdoor ... Compared to traditional methods of fungal exposure assessment, ... Fungal diversity was determined by sequencing the internal ... Internal transcribed spacer rRNA gene sequencing analysis of fungal diversity in Kansas City indoor environments.. ...
But in very rare cases, fungal infections can spread below the skins surface and ... ... a fungal infection like athletes foot means a simple trip to the drugstore and a reminder to bring shower shoes to the gym. ... Gene linked to deadly runaway fungal infection. October 21, 2013, Rockefeller University ... The HLF gene protects blood stem cells by maintaining them in a resting state. January 17, 2018 The HLF gene is necessary for ...
Heterologous expression of wood-rotting and litter-decomposing fungal genes involved in lignin degradation. From 2009-03-01 to ... Final Report Summary - FUNGEN (Heterologous expression of wood-rotting and litter-decomposing fungal genes involved in lignin ... Heterologous expression of wood-rotting and litter-decomposing fungal genes involved in lignin degradation ... In this project ligninolytic enzymes of the litter-decomposing fungi and fungal oxalate decarboxylase, an essential part of ...
... ... 27th Fungal Genetics Conference, 2013-03-12/2013-03-17. Department(s). Laboratory of Phytopathology. PRI BIOINT Moleculair ... In the current study, we functionally analysed these three catalase-peroxidase genes and found that MgCatD-1, encoding a ... Similar to many plant pathogens, Z. tritici possesses three catalase-peroxidase genes that are known to detoxify H2O2 ...
We have screened the available protein and DNA databases to identify putative peroxin orthologs in 17 fungal species (yeast and ... PEX genes encode proteins, termed peroxins, that are required for the biogenesis and proliferation of microbodies (peroxisomes ... PEX genes in fungal genomes: common, rare or redundant Traffic. 2006 Oct;7(10):1291-303. doi: 10.1111/j.1600-0854.2006.00479.x ... Furthermore, we found that the Y. lipolytica PEX9 gene, a putative orphan gene, might encode a Pex26p ortholog. In addition, in ...
Developmentally regulated cell death on expression of the fungal avirulence gene Avr9 in tomato seedlings carrying the disease- ... Developmentally regulated cell death on expression of the fungal avirulence gene Avr9 in tomato seedlings carrying the disease- ... Developmentally regulated cell death on expression of the fungal avirulence gene Avr9 in tomato seedlings carrying the disease- ... Developmentally regulated cell death on expression of the fungal avirulence gene Avr9 in tomato seedlings carrying the disease- ...
Main conclusion Expressing an RNAi construct in maize kernels that targets the gene for alpha-amylase in Aspergillus flavus ... resulted in suppression of alpha-amylase ( amy1) gene expression and... ... flavus alpha-amylase gene amy1 effectively reduces amy1 gene expression resulting in decreased fungal colonization and ... The ribosomal structural gene from maize and A. flavus beta-tubulin gene showed no reaction could be detected by SYBR qPCR when ...
PLS1, a gene encoding a tetraspanin-like protein, is required for penetration of rice leaf by the fungal pathogen Magnaporthe ... PLS1, a gene encoding a tetraspanin-like protein, is required for penetration of rice leaf by the fungal pathogen Magnaporthe ... PLS1, a gene encoding a tetraspanin-like protein, is required for penetration of rice leaf by the fungal pathogen Magnaporthe ... PLS1, a gene encoding a tetraspanin-like protein, is required for penetration of rice leaf by the fungal pathogen Magnaporthe ...
... and JA-induced rice gene (JAmyb) that encodes a Myb transcription factor. The JAmyb gene was induced within 1 day after fungal ... A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death.. Lee MW1, Qi M, Yang Y. ... Unlike most defense-related genes that are activated faster and stronger in resistant interactions, JAmyb induction by blast ...
Research on fungal nitrilase gene expression will advance our understanding for its biological function of fungal nitrilase in ... Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties.. [Jin ... A fungal nitrilase gene from Gibberella intermedia was cloned through reverse transcription-PCR. The open reading frame ... The encoding gene was expressed in Escherichia coli Rosetta-gami (DE3) and the recombinant protein with His(6)-tag was purified ...
In order to identify the gene(s) encoding the protein responsible for the lipolytic activity, in silico identification and ... The success in identifying a lipase gene inducible with olive oil and the suitability of the functional screening and ... The gene codifying 526309 was expressed when the fungus grew in a medium with olive oil as carbon source. This protein shares ... A lipolytic screening with fungal strains isolated from lignocellulosic waste collected in banana plantation dumps was carried ...
... fungal blast (BL) and the insect Asian rice gall midge (GM) that cause significant yield... ... Genes Involved in Rice BB Interaction. Two of the genes reported to be involved in rice-BB interaction, PR10a gene and ... While 21 out of 22 cloned R genes against BL and two of the three cloned R genes against GM are NB-LRR class of R genes, only ... Expression Profile of Defense Genes in Rice Lines Pyramided with Resistance Genes Against Bacterial Blight, Fungal Blast and ...
We conducted a gene replacement experiment to test if mutant phenotypes in Z. tritici could be complemented by the insertion of ... For two genes, we confirm that the species-specific protein changes are essential for proper protein functioning in Z. tritici ... We demonstrate that three of these four candidate genes play an important role during host infection. Two mutants of Z. tritici ... We have identified a small set of genes showing signatures of positive selection. ...
The research should pave the way for new treatments for skin fungal infections, fungal infections on the face and many more. ... but what makes some people get fungal infections and others to remain free of the problems these microbes cause? It is possible ... that genetic mutations may put individuals at increased risk of contracting a fungal infection. Thats the conclusion of ... Further proof of this was obtained from animal models where mice lacking either of these genes were susceptible to fungal ...
These pea pathogenicity (PEP) genes are within 25 kb of each other and are located on a supernumerary chromosome. Altogether, ... Three genes that contribute to the ability of the fungus Nectria haematococca to cause disease on pea plants have been ... Genes determining pathogenicity to pea are clustered on a supernumerary chromosome in the fungal plant pathogen Nectria ... The biochemical function of only one of the genes is known with certainty. This gene, PDA1, encodes a specific cytochrome P450 ...
Gene Regulation Shifts Shed Light on Fungal Adaption in Plant Biomass Decomposers. Jiwei Zhang, Kevin A. T. Silverstein, Jesus ... These imply that gene regulatory shifts, not simply ROS innovations, were key to brown rot fungal evolution. These results not ... Gene Regulation Shifts Shed Light on Fungal Adaption in Plant Biomass Decomposers ... Gene Regulation Shifts Shed Light on Fungal Adaption in Plant Biomass Decomposers ...
... rapid and reliable tool to detect and quantify fungal abundance using the β-actin gene, which is known to appear as a single- ... showing the lowest fungal CFU and qPCR values. In contrast, insulations made of wood showed to be not recommendable from the ... copy gene in fungi. To this end, five different indoor thermal insulation materials applied for historical buildings that were ... which is known to appear as a single-copy gene in fungi. To this end, five different indoor thermal insulation materials ...
... and JA-induced rice gene (JAmyb) that encodes a Myb transcription factor. The JAmyb gene was induced within 1 day after fungal ... Unlike most defense-related genes that are activated faster and stronger in resistant interactions, JAmyb induction by blast ...
  • NEW YORK (GenomeWeb News) - A Duke University research team has pinned down gene expression signatures in mice that coincide with bloodstream infections caused by the fungal pathogen Candida albicans . (genomeweb.com)
  • Here we present the genomic sequences of 182 C . albicans isolates collected worldwide, including commensal isolates, as well as ones responsible for superficial and invasive infections, constituting the largest dataset to date for this major fungal pathogen. (nature.com)
  • In the first study, scientists examined the genome of the obligate biotroph Pneumocystis jirovecii , a fungal pathogen found in human lungs. (icr.org)
  • Like the fungal pathogen in the first study, it is also a biotroph. (icr.org)
  • Plant defense responses are induced when the products of disease-resistance genes and pathogen avirulence genes interact. (pnas.org)
  • To gain further insights into JA-mediated defense signaling pathways, we isolated and characterized a pathogen- and JA-induced rice gene (JAmyb) that encodes a Myb transcription factor. (nih.gov)
  • Resistance often follows the gene-for-gene model in which plant resistance ( R ) gene products recognize avirulence (AVR) proteins, a subset of pathogen-secreted virulence proteins known as effectors, to trigger hypersensitive cell death and immunity. (plantcell.org)
  • Dendritic cell-associated C-type lectin-1 (Dectin-1) and dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) play a crucial role in the early procedure of fungal pathogen defenses. (molvis.org)
  • Some R genes mediate early and rapid responses that arrest pathogen growth with no macroscopic symptoms. (plantphysiol.org)
  • Other R genes allow growth for a short time until pathogen recognition occurs, at which time a macroscopic hypersensitive response develops. (plantphysiol.org)
  • We study the clinically important fungal pathogen Candida albicans , which affects a large proportion of the population by causing both superficial and invasive infections. (edu.au)
  • Projects are available in the areas of host-pathogen interactions and gene regulation. (edu.au)
  • Jiyoti works on the gene expression control mechanisms that regulate host-pathogen interactions and infection. (edu.au)
  • HIGS: host-induced gene silencing in the obligate biotrophic fungal pathogen Blumeria graminis. (semanticscholar.org)
  • Arabidopsis pen1-1 mutant lacking the PEN1 gene is penetrated by the hemibiotrophic oomycete pathogen Phytophthora sojae, the causal organism of the root and stem rot disease in soybean. (biomedcentral.com)
  • The P. s ojae s usceptible ( pss ) 1 mutant was identified by screening a mutant population created in the Arabidopsis pen1-1 mutant that lacks penetration resistance against the non adapted barley biotrophic fungal pathogen, Blumeria graminis f. sp. (biomedcentral.com)
  • The pss1 mutant is also infected by the necrotrophic fungal pathogen, Fusarium virguliforme, which causes sudden death syndrome in soybean. (biomedcentral.com)
  • The study revealed that Arabidopsis PSS1 is a novel nonhost resistance gene that confers a new form of nonhost resistance against both a hemibiotrophic oomycete pathogen, P. sojae and a necrotrophic fungal pathogen, F. virguliforme that cause diseases in soybean. (biomedcentral.com)
  • The first gene known to confer Arabidopsis NHR against a non adapted bacterial pathogen, Pseudomonas syringae pv. (biomedcentral.com)
  • Three NHR genes, PEN1, PEN2 and PEN3 , required for penetration resistance of Arabidopsis against the non adapted barley biotrophic fungal pathogen, Blumeria graminis f. sp. (biomedcentral.com)
  • Although Aspergillus fumigatus is an important human fungal pathogen there are few expression systems available to study the contribution of specific genes to the growth and virulence of this opportunistic mould. (biomedcentral.com)
  • The completion of the annotated sequence of the A. fumigatus genome is expected to greatly facilitate efforts to determine the contribution of specific gene products to the virulence of this opportunistic pathogen. (biomedcentral.com)
  • The wheat pathogen Stagonospora nodorum produces multiple necrotrophic effectors (also called host-selective toxins) that promote disease by interacting with corresponding host sensitivity gene products. (edu.au)
  • Parastagonospora nodorum is a major fungal pathogen of wheat in Australia causing septoria nodorum blotch (SNB). (edu.au)
  • Parastagonospora nodorum is a necrotrophic fungal pathogen that causes Septoria nodorum blotch (SNB) (formerly Stagonospora nodorum blotch) on wheat. (edu.au)
  • Homology-based screens were performed to identify catalase genes in the fungal tomato pathogen Cladosporium fulvum. (edu.au)
  • A retrotransposon from the fungal tomato pathogen Cladosporium fulvum (syn. (edu.au)
  • High-resolution genetic markers were used to assess the genetic diversity of 54 fungal isolates from five South Korean provinces, and results suggest that this fungal pathogen was introduced to South Korea. (fed.us)
  • The whole of creation was then subjected to a negative turn of events, including the emergence of fungal pathogens that cause death and disease in plants and animals. (icr.org)
  • Recently, several new studies were published showing how fungal pathogens adapted to their hosts through large-scale genomic changes involving a type of genome degradation marked by gene losses. (icr.org)
  • Similar to many plant pathogens, Z. tritici possesses three catalase-peroxidase genes that are known to detoxify H2O2 accumulated in the foliage during colonization. (wur.nl)
  • Is the Subject Area "Plant fungal pathogens" applicable to this article? (plos.org)
  • As do most fungal plant pathogens, M. grisea differentiates an infection structure specialized for host penetration called the appressorium. (pnas.org)
  • Several of these fungal pathogens depend on the formation of a specialized cell, called an appressorium, for the successful penetration of host plant surfaces ( 1 , 2 ). (pnas.org)
  • A few of these classes are significantly more prevalent in the fungal pathogens than in the saprobe N. crassa , suggesting that these groups contain paralogs required for virulence. (asm.org)
  • Like bacteria, many filamentous fungi are significant plant or human pathogens, and analysis of fungal HKs may result in key information about the biology of these pathogens. (asm.org)
  • These two genes may belong to a class of proteins specific for filamentous fungi and function as novel virulence factors in fungal pathogens. (plantcell.org)
  • Broekaert, Willem 2004-10-06 00:00:00 The plant defensin PDF1.2 has previously been shown to accumulate systemically via a salicylic acid-independent pathway in leaves of Arabidopsis upon challenge by fungal pathogens. (deepdyve.com)
  • Challenge of the transgenic plants with the fungal pathogens Alternaria brassicicola and Botrytis cinerea resulted in both local and systemic induction of the reporter gene. (deepdyve.com)
  • pathway in lea ves of Arabi dopsis upon challenge by fungal pathogens. (deepdyve.com)
  • Barley stripe mosaic virus-mediated tools for investigating gene function in cereal plants and their pathogens: virus-induced gene silencing, host-mediated gene silencing, and virus-mediated overexpression of heterologous protein. (semanticscholar.org)
  • Thus, a common NHR mechanism is operative in Arabidopsis against both hemibiotrophic oomycetes and necrotrophic fungal pathogens that are pathogenic to soybean. (biomedcentral.com)
  • Identification and further characterization of the PSS1 gene would provide further insights into a new form of nonhost resistance in Arabidopsis, which could be utilized in improving resistance of soybean to two serious pathogens. (biomedcentral.com)
  • Although there have been sporadic and persistent reports of lateral gene transfer between and into fungal plant pathogens, most examples have been dismissed through incomplete evidence. (edu.au)
  • The completion of whole genome sequences of an increasing number of fungal pathogens no longer allows such proposed cases of lateral gene transfer to be dismissed so easily. (edu.au)
  • How frequent are lateral gene transfers involving fungal plant pathogens, and can this process explain the emergence of many of the new diseases of the recent past? (edu.au)
  • Molecular genetics and cell biology techniques are combined to examine growth and development in model fungi and to determine the mechanisms by which plants resist attack by bacterial and fungal pathogens and by animals. (ed.ac.uk)
  • Hydrolytic enzymes such as chitinases and glucanases are implicated in plant defense responses against fungal pathogens. (edu.au)
  • Genes encoding these enzymes have been used to genetically engineer plants to enhance their protection against fungal pathogens. (edu.au)
  • Activity was determined by measuring reducing sugars in the fungal supernatants against cell wall preparations of six economically important plant pathogens. (edu.au)
  • Fusarium are among the most economically important fungal pathogens because they are ubiquitous, disease management remains a challenge, they produce mycotoxins that affect food and feed safety, and trichothecene mycotoxin production can increase the pathogenicity of some Fusarium species depending on the host species. (mdpi.com)
  • Fungal Disease Resistance in Plants is your guide to understanding the various barriers that plants have developed through evolution and adaptation to protect themselves from invading fungal pathogens. (routledge.com)
  • Among the latter, Candida albicans belongs to one of the four genera causing high-mortality rates in humans and is the second most frequent agent of fungal infection worldwide 4 . (nature.com)
  • For most people, a fungal infection like athlete's foot means a simple trip to the drugstore and a reminder to bring shower shoes to the gym. (medicalxpress.com)
  • Their work suggests why treatments for fungal infection sometimes fail, and it gives weight to a genetic theory of infectious diseases, which proposes that a single genetic defect can cause an otherwise healthy person to become severely ill from a minor infection-be it viral, bacterial or fungal. (medicalxpress.com)
  • Casanova and his team say that further research on CARD9 will examine its exact role in human anti-fungal immunity, tracing the signaling pathways in different cell types that give an individual the ability to fight off an infection. (medicalxpress.com)
  • There are a number of reasons that can contribute to a person getting a fungal infection and these include having a depressed immune system, poor circulation, prolonged use of antibiotics, and coming into contact with contaminated water. (brighthub.com)
  • It is possible that genetic mutation s may put individuals at increased risk of contracting a fungal infection. (brighthub.com)
  • Altogether, the PEP gene cluster contains six transcriptional units that are expressed during infection of pea tissue. (nih.gov)
  • Expressing an RNAi construct in maize kernels that targets the gene for alpha-amylase in Aspergillus flavus resulted in suppression of alpha-amylase ( amy1 ) gene expression and decreased fungal growth during in situ infection resulting in decreased aflatoxin production. (springer.com)
  • To isolate genes involved in this infection process, we generated a collection of M. grisea pathogenicity mutants by using plasmid-mediated insertional mutagenesis. (pnas.org)
  • A novel jasmonic acid-inducible rice myb gene associates with fungal infection and host cell death. (nih.gov)
  • The JAmyb gene was induced within 1 day after fungal infection in resistant and susceptible interactions prior to lesion formation. (nih.gov)
  • We developed and optimized six real-time PCR assays in order to determine the copy number of four mitochondrial genes ( mtSSU rRNA , mtLSU rRNA , NAD1 , and CYTB ) in comparison to nuclear genome ( DHPS and HSP70 ) and tested 84 bronchoalveolar fluids of patients at different stages of the infection. (frontiersin.org)
  • This appears to be the first case of producing transgenic plant tissue with a reduced ability to produce a phytoalexin and demonstrating that such tissue is less resistant to fungal infection: these results support the hypothesis that phytoalexin production is a disease resistance mechanism. (apsnet.org)
  • C-type lectin receptors (CLRs) are members of PRRs and are important in the response to fungal infection. (molvis.org)
  • However, each has significant limitations that are especially problematic in pathogenic microbes when it is desirable to determine if a particular gene is required for infection in an animal model. (asm.org)
  • In phytopathogenic fungi, the expression of hundreds of small secreted protein (SSP)-encoding genes is induced upon primary infection of plants while no or a low level of expression is observed during vegetative growth. (semanticscholar.org)
  • A quantitatively different expression of SSP-encoding genes compared to plant infection was also detected. (semanticscholar.org)
  • To investigate the association between dectin-1 gene single nucleotide polymorphisms (SNPs) and susceptibility to fungal infection (FI). (cdc.gov)
  • How do you fight a fungal infection that is becoming increasingly resistant to medicine? (news-medical.net)
  • Candida albicans is one of the most formidable fungal species, causing infection in humans. (pasteur.fr)
  • It belongs to one of the four genera of pathogenic fungi responsible for high mortality rates in humans and is the second most common agent of opportunistic fungal infection in the world. (pasteur.fr)
  • Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinerea. (wur.nl)
  • In the current study, transcriptome analyses of the fungus and its host were conducted during discrete stages of bud development so as to identify changes in fungal gene expression that lead to spore development and to identify changes associated with infection in the host plant. (g3journal.org)
  • During M. lychnidis-dioicae infection of S. latifolia male flowers, dikaryotic fungal hyphae penetrate the host tissue and proceed through the plant causing little or no tissue damage. (g3journal.org)
  • Fungal Disease Resistance in Plants: Biochemistry, Molecular Biology, and Genetic Engineering presents the latest developments in crop protection from fungal infection. (routledge.com)
  • When the researchers compared the genome of this fungus to the fungus from which they believed it was derived, they claimed that it "lost 2,324 genes in relation to the acquisition of obligate biotrophy. (icr.org)
  • Now it looks like we can add gene loss (an aspect of genome degradation) as a key part of the creationist model-explaining genetic mechanisms that underlie the curse on creation. (icr.org)
  • Several of the features of the PEP cluster -- a cluster of pathogenicity genes, the presence of transposable elements, and differences in codon usage and GC content from other portions of the genome -- are shared by pathogenicity islands in pathogenic bacteria of plants and animals. (nih.gov)
  • Unexpectedly, we found that copy number of mitochondrial genes varied from gene to gene with mtSSU rRNA gene being more represented (37 copies) than NAD1 (23 copies), mtLSU rRNA (15 copies) and CYTB (6 copies) genes compared to nuclear genome. (frontiersin.org)
  • C. heterostrophus (yellow, Ch), G. moniliformis (blue, Gm), and B. fuckeliana (green, Bf) sequences were obtained by homology and splice consensus-based manual predictions from TMRI fungal genome sequences. (asm.org)
  • To isolate novel avirulence genes from M. oryzae , we examined DNA polymorphisms of secreted protein genes predicted from the genome sequence of isolate 70-15 and looked for an association with AVR activity. (plantcell.org)
  • Therefore, we used genome resequencing of Ina168, an M. oryzae isolate that contains nine AVR genes. (plantcell.org)
  • Several approaches designed to partially or completely abolish the function of a gene, including its deletion from the genome and the use of systems that facilitate conditional expression, have been widely applied. (asm.org)
  • Notably, analysis of the phylum Saccharomycotina has revealed major events of evolution such as the recent genome duplication and subsequent gene loss. (biomedcentral.com)
  • We have carried out a computational genome wide comparison of protein coding gene content of Saccharomycotina and Pezizomycotina, which include industrially important yeasts and filamentous fungi, respectively. (biomedcentral.com)
  • Comparative genomics has been applied extensively within the phylum Saccharomycotina (for review see [ 1 ]), where a whole genome duplication followed by massive gene loss and specialization has been shown to have occurred [ 2 ]. (biomedcentral.com)
  • The increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. (biomedcentral.com)
  • Studies of the yeast Saccharomyces cerevisiae reveal four processes determining life span: metabolism, stress resistance, chromatin-dependent gene regulation, and genome stability. (isharonline.org)
  • Multiple copies of the translation elongation factor 1-a ( tefA ) and β-tubulin ( tub2 ) 'house-keeping' genes, as well as the endophyte-specific perA gene, were identified for each fescue-derived endophyte taxon from whole genome sequence data. (biomedcentral.com)
  • In addition to the nuclear genome-derived genes, the complete mitochondrial genome (mt genome) sequence was obtained for each of the sequenced endophyte, and phylogenetic relationships between the mt genome protein coding gene complements were also reconstructed. (biomedcentral.com)
  • Complex and highly reticulated evolutionary relationships between Epichloë-Neotyphodium endophytes have been predicted on the basis of multiple nuclear genes and entire mitochondrial protein-coding gene complements, derived from independent assembly of whole genome sequence reads. (biomedcentral.com)
  • Horizontal Gene Transfer (HGT) is an exchange of genetic material between different strains or species ultimately integrated into the genome. (biomedcentral.com)
  • Motivation: Successful genome analysis depends on the quality of gene prediction. (elsevier.com)
  • Although fungal genome sequencing and assembly have become trivial, its annotation procedure has not been standardized yet. (elsevier.com)
  • Results: FunGAP predicts protein-coding genes in a fungal genome assembly. (elsevier.com)
  • The research has benefitted greatly from the recent sequencing of the wheat genome, effectively providing a DNA map of the structure and function of genes that provide resistance to SNB. (phys.org)
  • Based on the genome sequence and gene expression profiles we will identify genes involved in the parasitic interaction of Armillaria ostoyae with its hosts. (wsl.ch)
  • Genome sequences can be used to expand the number of genes used in phylogenetic analyses, but many publicly available genomes lack gene annotations and popular rDNA markers are typically missing from genomic sequences or are incorrectly assembled. (wikipedia.org)
  • In addition, in the genomes of Saccharomyces cerevisiae and Candida glabrata, several PEX genes appear to have been duplicated, exemplified by the presence of paralogs of the peroxins Pex5p and Pex21p, which were absent in other organisms. (nih.gov)
  • The success in identifying a lipase gene inducible with olive oil and the suitability of the functional screening and bioinformatic survey carried out herein, support the premise that the strategy can be used in other microorganisms with sequenced genomes to search for true lipases, or other enzymes belonging to large protein families. (mdpi.com)
  • At least 36 fungal genomes are already available at public databases and several more are being sequenced. (biomedcentral.com)
  • The sequenced genomes cover fungal species broadly and include industrially, medically and agriculturally important species with very diverse genomes. (biomedcentral.com)
  • For these reasons fungal genomes form one of the most attractive eukaryotic datasets for comparative genomics research and method development. (biomedcentral.com)
  • A dataset of 345,829 genes was extracted from 42 publicly available fungal genomes. (biomedcentral.com)
  • The assembled gene sequences were used to reconstruct evolutionary relationships between the heteroploid fescue-derived endophytes and putative ancestral sub-genomes derived from known sexual Epichloë species. (biomedcentral.com)
  • The results are consistent with previous studies while also providing novel phylogenetic insights, particularly through inclusion of data from the endophyte lineage-specific gene, as well as affording evidence for the origin of cytoplasmic genomes. (biomedcentral.com)
  • They are also found in plant and fungal genomes. (biomedcentral.com)
  • The recent availability of a large number of fungal genomes represents an opportunity to investigate how genes involved in diterpene synthesis were acquired by fungi, and to assess the consequences of this process on the fungal metabolism. (biomedcentral.com)
  • Scientists in the Biology and Fungal Pathogenicity Unit at the Institut Pasteur and INRA, in collaboration with 12 other teams, sequenced and analyzed the genomes of 182 strains of C. albicans isolates, either commensal or responsible for superficial or invasive infections, from around the world. (pasteur.fr)
  • Here, a gene cluster ( vdt ) responsible for viriditoxin synthesis was identified, via a bioinformatics analysis of the genomes of Paecilomyces variotii and Aspergillus viridinutans that both are viriditoxin producers. (biomedcentral.com)
  • Fungal genomes are among the smallest genomes of eukaryotes. (wikipedia.org)
  • The sizes of fungal genomes range from less than 10 Mbp to hundreds of Mbp. (wikipedia.org)
  • The sizes and gene numbers of the smallest genomes of free-living fungi such as those of Wallemia ichthyophaga, Wallemia mellicola or Malassezia restricta are comparable to bacterial genomes. (wikipedia.org)
  • Due to their compact size fungal genomes can be sequenced with less resources than most other eukaryotic genomes and are thus important models for research. (wikipedia.org)
  • The comparison of fungal genomes has been used to study the evolution of fungi, to improve the resolution of the phylogeny of fungal species, and to determine the time of the emergence and changes in species traits and lifestyles, such as the evolution symbiotic or pathogenic interactions, and the evolution of different morphologies. (wikipedia.org)
  • While genomic distance measures such as the average nucleotide identity (ANI) are used routinely to distinguish bacterial species, the use of fungal genomes in taxonomy is currently rare. (wikipedia.org)
  • For example, a fixed threshold genomic distance calculated tools such as Mash and Dashing was able to determine whether two genomes belong to the same or to different species with over 90% accuracy, indicating that simple measures of genomic distance might be useful to delineate fungal species and still largely support the existing fungal taxonomy. (wikipedia.org)
  • So how could a loss of genes contribute to this fungus becoming a renegade parasite living off its human host? (icr.org)
  • 2014. Comparative Genomics Suggests That the Human Pathogenic Fungus Pneumocystis jirovecii Acquired Obligate Biotrophy through Gene Loss. (icr.org)
  • 2014. Gene Loss Rather Than Gene Gain Is Associated with a Host Jump from Monocots to Dicots in the Smut Fungus Melanopsichium pennsylvanicum. (icr.org)
  • Using the model filamentous fungus Neurospora crassa , we identified two genes ( qc-1 and qc-2 ) that encode proteins homologous to mammalian glutaminyl cyclases. (osti.gov)
  • Saline-Dependent Regulation of Manganese Peroxidase Genes in the Hypersaline-Tolerant White Rot Fungus Phlebia sp. (asm.org)
  • The gene codifying 526309 was expressed when the fungus grew in a medium with olive oil as carbon source. (mdpi.com)
  • Three genes that contribute to the ability of the fungus Nectria haematococca to cause disease on pea plants have been identified. (nih.gov)
  • The three new PEP genes, in addition to PDA1, can independently increase the ability of the fungus to cause lesions on pea when added to an isolate lacking the supernumerary chromosome. (nih.gov)
  • Host-induced gene silencing, whereby the host crop produces siRNA molecules targeting crucial genes in the invading fungus and targeting the gene for degradation, has shown to be promising in its ability to inhibit fungal growth and decrease aflatoxin contamination. (springer.com)
  • Unlike most defense-related genes that are activated faster and stronger in resistant interactions, JAmyb induction by blast fungus is much higher in susceptible interactions, accompanied by large lesions and extensive tissue damage. (nih.gov)
  • PDA , a gene encoding pisatin demethylating activity (pda) in the pea-pathogenic fungus Nectria haematococca, also was used in an attempt to reduce pisatin levels. (apsnet.org)
  • The PMK1 mitogen-activated protein kinase gene regulates appressorium formation and infectious hyphae growth in the rice blast fungus. (plantcell.org)
  • Two genes identified in this library, GAS1 and GAS2 , encode small proteins that are homologous with gEgh16 of the powdery mildew fungus. (plantcell.org)
  • Rice blast, which is caused by this heterothallic haploid fungus, is one of the most severe fungal diseases of rice throughout the world ( Valent, 1990 ). (plantcell.org)
  • Molecular identification of M. oryzae AVR genes will tremendously facilitate race identification of blast fungus and help rapid and effective deployment of R genes in rice cultivation. (plantcell.org)
  • Two partial chitinase genes from the soil filamentous ungus Trichoderma asperellum,(ChiA and ChiB) and a novel glucanase gene from the filamentous fungus Aspergillus (Glu1) were cloned. (edu.au)
  • whereby the plant is resistant to a first fungus expressing at least one gene having substantial homology to the first antisense sequence. (patentsencyclopedia.com)
  • 3. The method of claim 2, wherein the second pest pathogenicity gene is a fungus pathogenicity gene, whereby the plant is resistant to a second fungus expressing at least one gene having substantial homology to the second antisense sequence. (patentsencyclopedia.com)
  • 6. The method of claim 1, wherein the first fungus can cause a fungal disease selected from the group consisting of brown stem rot, stem and root rot, white mold disease, sudden death syndrome, Asian rust, and downy mildew. (patentsencyclopedia.com)
  • The results obtained here suggest that fungal di-TPSs originated from an ancient HGT event of a single di-TPS gene from a plant to a fungus in Ascomycota. (biomedcentral.com)
  • Genomic sequencing of the fungus provided evidence of genes associated with causing tree disease, and allowed comparisons with related fungi. (fed.us)
  • In the filamentous fungus Neurospora crassa , genes unpaired during meiosis are silenced by a process known as meiotic silencing by unpaired DNA (MSUD). (g3journal.org)
  • In this project we aim to compare the virulence of North American and South Korean genotypes of the chestnut blight fungus Cryphonectria parasitica, which have never encountered the European chestnut, with that of European fungal genotypes: Are the non-European genotypes more virulent? (wsl.ch)
  • This fungus is part of a species complex that infects species of the Caryophyllaceae, replacing pollen with the fungal spores. (g3journal.org)
  • The cellulolytic and xylanolytic capacity of rumen fungi was also studied in detail by characterizing the enzyme encoding genes. (scielo.br)
  • In this project ligninolytic enzymes of the litter-decomposing fungi and fungal oxalate decarboxylase, an essential part of effective lignin degradation, will be heterologously produced and characterised. (europa.eu)
  • Fungal diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA derived from fungi collected in air and dust samples from 31 homes participating in the Kansas City Safe and Healthy Homes Program (KCSHHP). (cdc.gov)
  • We have screened the available protein and DNA databases to identify putative peroxin orthologs in 17 fungal species (yeast and filamentous fungi) and in humans. (nih.gov)
  • When both these genes are functioning normally Dectin-1 senses the presence of fungi and prompts the immune cells to send signals and mount a response. (brighthub.com)
  • The scientists discovered that if there is a mutation in any one of these genes then the body struggles to control the fungi. (brighthub.com)
  • Dozens of "decay-stage-dependent" ortho-genes were isolated, narrowing a pool of candidate genes with time-dependent regulation unique to brown rot fungi. (asm.org)
  • In this study, we developed a real time PCR method as a simple, rapid and reliable tool to detect and quantify fungal abundance using the β-actin gene, which is known to appear as a single-copy gene in fungi. (frontiersin.org)
  • The genes found in all fungi except Saccharomycotina are slightly better characterised and predicted to encode mainly enzymes. (biomedcentral.com)
  • Here, we provide direct evidence of integration by sequencing across host-virus gene boundaries and carry out phylogenetic analyses of fungal hosts and totivirids (dsRNA viruses of fungi and protozoans). (blogspot.com)
  • At present, these Ecl1 family genes, which are extenders of chronological lifespan, have been identified only in fungi groups. (isharonline.org)
  • The recent development of a microarray that has subarrays for the gene clusters of key mycotoxigenic fungi has provided an excellent tool for examining in more detail the impact that changes in interacting environmental factors may have on the relative expression of these gene clusters and relating this to phenotypic mycotoxin production [ 14 , 15 ]. (royalsocietypublishing.org)
  • The aims of this research project were to isolate chitinolytic and glucanolytic fungi from soil and to clone the genes encoding for chitinase and glucanase enzymes. (edu.au)
  • To achieve these aims, fungi with activity against chitin and glucan were isolated, the activity quantified by colorimetric and inhibition assays and gene fragments with homology to known chitinase and glucanase genes were isolated and their sequences determined. (edu.au)
  • To address the question whether both fungi have undergone similar genetic adaptions, we have performed a comparative gene expression analysis of wild-type and production strains. (biomedcentral.com)
  • Common in both fungi is the upregulation of genes belonging to primary and secondary metabolism, notably those involved in precursor supply for β-lactam production. (biomedcentral.com)
  • pcbAB and pcbC, the genes coding for the first two enzymes involved in β-lactam biosynthesis, are genomically clustered in both bacteria and fungi. (biomedcentral.com)
  • This clustered organization and the lack of intronic sequences in most of the genes led to the assumption that bacterial β-lactam antibiotic genes were transferred horizontally from bacteria to fungi [ 5 ]. (biomedcentral.com)
  • Cat2 codes for a putative catalase subunit of 745 amino acids and belongs to a different gene family coding for the large-subunit catalases similar to ones found in bacteria and filamentous fungi. (edu.au)
  • Further evidence for HGT was provided by conservation of synteny of di-TPS and neighbouring genes in distantly related fungi. (biomedcentral.com)
  • In fungi, these di-TPSs allowed for the formation of clusters consisting in di-TPS, GGPPS and P450 genes to create functional clusters that were transferred between fungal species, producing diterpenes acting as hormones or toxins, thus affecting fungal development and pathogenicity. (biomedcentral.com)
  • The transfer of genes between plants and fungi is also possible [ 10 - 12 ], and the transfer of a nitrate assimilation cluster from the Oomycota to an ancestral Dikarya species was also recently documented [ 13 ]. (biomedcentral.com)
  • To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4′-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation in A. nidulans, as a new reporter gene. (bvsalud.org)
  • The functional organization of serially deleted promoter regions of the A. nidulans trpC gene and the Cryphonectria parasitica crp gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase npgA gene. (bvsalud.org)
  • Fungi produce a diverse array of polyketide-derived biaryl compounds with biological activities that are of interest as pharmaceutical lead molecules or through modulating fungal interactions with other species in the environment. (biomedcentral.com)
  • Despite the absence of meiosis, the parasexual cycle of C. albicans allows chromosome shuffling and recombination events by means of gene conversion and mitotic recombination, likely contributing to the genetic and phenotypic diversity in this species 5 , 6 . (nature.com)
  • ITS sequencing revealed the presence of a number of rarely documented fungal species placed in the Pleosporales. (cdc.gov)
  • This redundancy may complicate future studies on peroxisome biogenesis and proliferation in fungal species. (nih.gov)
  • We applied comparative transcriptomics to multiple species of both rot types grown across a wood wafer to create a gradient of progressive decay and to enable tracking temporal gene expression. (asm.org)
  • Traditional methods of systematics based on morphology of vegetative cells, sexual states, physiological responses to fermentation and growth tests can assign fungal species to particular genera and families. (biomedcentral.com)
  • Inducible promoter systems have proven to be instrumental for the elucidation of gene function in a number of species, most notably with essential genes. (biomedcentral.com)
  • Neotyphodium endophytes are asexual fungal species that form mutualistic interactions with a number of cool-season grasses, including ryegrasses ( Lolium spp. (biomedcentral.com)
  • In some species such as Leptosphaeria maculans, this coordinated in-planta upregulation of SSP-encoding genes expression relies on an epigenetic control but the signals triggering gene expression in-planta are unknown. (semanticscholar.org)
  • Western Australia has over 4000 endemic plant species and a largely unknown fungal biota. (edu.au)
  • Twenty four chitinolytic and glucanolytic fungal isolates were identified by growing them on chitin-containing media to select for those species that utilised chitin/glucan as a carbon source. (edu.au)
  • Cat1 codes for a putative 566-amino-acid catalase subunit and belongs to the gene family that also encodes the mainly peroxisome-localized catalases of animal and yeast species. (edu.au)
  • In order to investigate the origin of fungal di-TPS, we implemented a search for potential fungal di-TPS genes and identified their presence in several unrelated Ascomycota and Basidiomycota species. (biomedcentral.com)
  • While exploring this diversity, we discovered that only half of the known marine fungal species have a publicly available DNA locus, which we hypothesize will likely hinder accurate high-throughput sequencing classification in the future, as it does currently. (degruyter.com)
  • There are 5 million fungal species, but only a few hundred can cause disease in humans. (pasteur.fr)
  • Due to the challenges of inducing trichothecene production by Fusarium isolates in vitro for subsequent chemical analysis, PCR assays using gene-specific primers, either singly or in combination, designed against specific genes of the trichothecene gene cluster of multiple species of Fusarium have been developed. (mdpi.com)
  • and (iv) based on (i)-(iii), selection of appropriate target TRI gene(s) for primer design in PCR amplification for the Fusarium species under study. (mdpi.com)
  • Transcriptome analysis via Illumina RNA sequencing revealed enrichment of fungal genes encoding small secreted proteins, with hallmarks of effectors and genes found to be relatively unique to the Microbotryum species complex. (g3journal.org)
  • Hence, this work reveals the basis for the synthesis of an understudied class of fungal secondary metabolites and provides a new model species for understanding the synthesis of biaryl compounds with a chiral axis. (biomedcentral.com)
  • Prevalence of individual Ascomycota orders in KCSHHP air and dust samples (A). Distribution of the most prevalent species determined in the ITS sequence analysis of air (left pie chart) and dust (right pie chart) samples for the fungal orders Pleosporales (B), Eurotiales (C), and Capnodiales (D). n , denotes the number of clones identified. (cdc.gov)
  • Prevalence of individual Basidiomycota orders in KCSHHP air and dust samples (A). Distribution of most prevalent species in the ITS sequence analysis of air (left pie chart) and dust (right pie chart) samples for the fungal orders Ustilaginales (B), Tremellales (C), and Polyporales (D). n , denotes the number of clones identified. (cdc.gov)
  • The pyroglutamate modification is predicted to occur in a number of additional fungal proteins that have diverse functions. (osti.gov)
  • PEX genes encode proteins, termed peroxins, that are required for the biogenesis and proliferation of microbodies (peroxisomes). (nih.gov)
  • Gene Expression is the process by which a gene gets turned on in a cell to make RNA and proteins. (news-medical.net)
  • With Cat2-deleted strains, activity of the Cat2 gene product (CAT2) was identified among four proteins with catalase activity separated on non-denaturing gels. (edu.au)
  • Dicer proteins are ribonuclease III enzymes that process double stranded RNA precursors into small RNAs categorized as small interfering RNAs (siRNAs) or microRNAs (miRNAs), which suppress gene expression through the RNA silencing mechanism. (nih.gov)
  • Fungal prions are naturally occurring proteins that can switch between multiple, structurally distinct conformations, at least one of which is self-propagating and transmissible to other prions. (wikipedia.org)
  • In order to identify the gene(s) encoding the protein responsible for the lipolytic activity, in silico identification and characterization of triacylglycerol lipases from T. harzianum is reported for the first time. (mdpi.com)
  • Supplementary material 2 (PNG 11 kb) Supplemental Fig. S2 Quantitative PCR analysis showing the relative expression levels of the A. flavus beta-tubulin gene normalized to the maize ribosomal protein L10 gene, GRMZM2G024838. (springer.com)
  • The inactivated gene, PLS1 , encodes a putative integral membrane protein of 225 aa (Pls1p). (pnas.org)
  • We show that the PLS1 gene is inactivated in punchless and encodes a putative membrane protein related to the tetraspanin family ( 10 - 12 ). (pnas.org)
  • The encoding gene was expressed in Escherichia coli Rosetta-gami (DE3) and the recombinant protein with His(6)-tag was purified to electrophoretic homogeneity. (sigmaaldrich.com)
  • To further characterize this mitogen-activated protein kinase pathway, we constructed a subtraction library enriched for genes regulated by PMK1 . (plantcell.org)
  • One mitogen-activated protein (MAP) kinase gene, PMK1 , was found to be essential for appressorium formation and infectious hyphae growth in Magnaporthe ( Xu and Hamer, 1996 ). (plantcell.org)
  • This large-scale study found significantly more presence/absence polymorphisms than nucleotide polymorphisms among 1032 putative secreted protein genes. (plantcell.org)
  • Trichoderma harzianum was cotransformed with genes encoding green fluorescent protein (GFP), β-glucuronidase (GUS), and hygromycin B ( hygB ) resistance, using polyethylene glycol-mediated transformation. (asm.org)
  • Rapid induction of a protein disulfide isomerase and defense-related genes in wheat in response. (deepdyve.com)
  • Among the differentially expressed genes was a protein disulfide isomerase (PDI), which is well known as a molecular chaperone and component of signal-transduction pathways in animal systems but had not been implicated previously in plant defense response. (deepdyve.com)
  • Traven A , Sopta M: Functional characterization of the fungal specific protein Taf14 in S. cerevisiae and C. albicans . (edu.au)
  • Our results confirm that this approach enables us to predictably and gradually titrate the expression level of a protein and thus to investigate the phenotypic consequences of various levels of gene/protein function. (asm.org)
  • Reporter genes include genes that code for fluorescent protein and enzymes that convert invisible substrates to luminescent or coloured products. (nature.com)
  • Imaging of living cells and electron microscopy are used to examine a number of questions, from cell-cell signalling in plant and fungal growth to protein targeting. (ed.ac.uk)
  • Researchers reduce the severity of hereditary deafness in mice with the delivery of CRISPR-Cas9 protein-RNA complexes that inactivate a mutant gene in their inner ears. (the-scientist.com)
  • Richard G. Pestell, M.D., Ph.D. and his research team have been studying the cyclin D1 gene and the protein it produces for the past decade. (scienceblog.com)
  • Gene expression may be measured by looking at the RNA, or the protein made from the RNA, or what the protein does in a cell. (news-medical.net)
  • New research from The Wistar Institute sheds light on the function of the ARID1A protein, encoded by a gene that is among the most frequently mutated across human cancers. (news-medical.net)
  • Bioinformatics Analysis of Upstream Region and Protein Structure of Fungal Phytase Gene', سامانه مدیریت نشریات علمی , 73(3), pp. 229-237. (ac.ir)
  • In this study, we sought to perform bioinformatics analysis of the upstream region and protein structure of fungal phytase to improve its expression and thermostability properties. (ac.ir)
  • To do this, we built a scoring function to estimate the congruency of each gene model based on known protein or domain homology. (elsevier.com)
  • In addition, we highlight several individual gene families with interesting phylogenetic distributions. (biomedcentral.com)
  • An alternative approach to a single gene phylogeny is to combine all available phylogenetic data. (biomedcentral.com)
  • Using the sequence of PcbC, a key enzyme of β-lactam antibiotic biosynthesis, from eighteen different pro- and eukaryotic microorganisms, we have constructed a phylogenetic tree to demonstrate the distant relationship of both fungal producers. (biomedcentral.com)
  • The fungal di-TPS phylogenetic tree is function-related but is not associated with the phylogeny based on housekeeping genes. (biomedcentral.com)
  • A highly clonal lineage, which experimentally shows reduced fitness, has undergone pseudogenization in genes required for virulence and morphogenesis, which may explain its niche restriction. (nature.com)
  • Barbara studies the roles of mitochondria and metabolic reprogramming in fungal virulence. (edu.au)
  • Bella studies a regulator of gene expression in Candida virulence. (edu.au)
  • Sharon is interested in signalling pathways that control fungal virulence. (edu.au)
  • Despite their agronomical importance, little direct functional evidence for genes of pathogenicity and virulence is currently available because mutagenesis and transformation protocols are lacking. (semanticscholar.org)
  • Importantly, to significantly strengthen her career the applicant will learn several novel and valuable techniques including gene array methodology, associated bioinformatics and molecular genetics approaches applied to Aspergillus spp. (europa.eu)
  • ENGLISH ABSTRACT: The aim of this study on grapevine was to genetically characterise, validate and map the reported fungal disease resistance genes of Pölöskei Muskotály (PM), Kishmish Vatkana (KV) and Villard Blanc (VB) in South Africa using QTL analysis. (sun.ac.za)
  • Several resistance genes in wheat have been identified, yet the molecular mechanisms of resistance are unknown. (deepdyve.com)
  • resistance genes in wheat ha ve been identified, yet th e molecular mechanisms of resist ance are unknow n. (deepdyve.com)
  • This study succeeded in genetically characterising the fungal disease resistance genes of two different sources of grapevine and provided exclusionary information on a third resistance source for future breeding applications. (sun.ac.za)
  • We mapped PSS1 to a region very close to the southern telomere of chromosome 3 that carries no known disease resistance genes. (biomedcentral.com)
  • Individuals with certain gene variants appear less able to resist Candida skin infections, explaining the observation that chronic mucocutaneous candidiasis (CMC) runs in families, researchers suggested. (medpagetoday.com)
  • Candida albicans is the second most frequent agent of human fungal infections worldwide, causing high-mortality rates. (nature.com)
  • Candida albicans thus takes advantage of both clonality and gene flow to diversify. (nature.com)
  • The Radboud team found a mutation in gene called Dectin-1 and this was linked to an increased susceptibility to vaginal infections by Candida. (brighthub.com)
  • Diagnosis and treatment of fungal infections remains very challenging, and life-threatening infections with Candida can have an astonishing mortality of 40% even with treatment. (edu.au)
  • Fungal determinants and host cell death signals in fatal Candida infections . (edu.au)
  • Escherichia coli strains were grown in Luria-Bertani (LB) broth in a shaking incubator (150 rpm) at 37 o C. The list of fungal and E. coli strains is given in Table 1 . (scielo.br)
  • A lipolytic screening with fungal strains isolated from lignocellulosic waste collected in banana plantation dumps was carried out. (mdpi.com)
  • Fungal Strains and Growth Conditions. (pnas.org)
  • P1.2 ( MAT 1.2) and M4 ( MAT 1.1) are M. grisea strains from the Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). (pnas.org)
  • however, the presence of the GFP and GUS marker genes permitted differentiation of introduced Trichoderma from indigenous strains. (asm.org)
  • However, it was not possible to differentiate the hyphal growth of this fungal agent from that of indigenous Trichoderma strains ( 3 , 18 ). (asm.org)
  • Finally, we constructed a series of C. albicans strains with 3 to 12 AAA codons inserted at the 5′ end of the ARO1 gene, which encodes a pentafunctional enzyme catalyzing five sequential steps of the aromatic amino acid biosynthetic pathway. (asm.org)
  • The effect of changes in temperature/water activity ( a w ) on growth, deoxynivalenol (DON) production and trichothecene gene cluster expression (18 genes) for strains of Fusarium culmorum and Fusarium graminearum was studied. (royalsocietypublishing.org)
  • Microarray data on expression of these genes were significantly related to DON production for both strains. (royalsocietypublishing.org)
  • It is so far unknown, whether equivalent changes in gene expression lead to elevated antibiotic titers in production strains. (biomedcentral.com)
  • In both deletion strains, approximately 50% of all secondary metabolite cluster genes are differentially regulated, including β-lactam biosynthesis genes. (biomedcentral.com)
  • Most importantly, 35-57% of Velvet target genes are among those that showed differential expression in both improved industrial strains. (biomedcentral.com)
  • Our comparative genomics identify that while plant biomass degrading genes (CAZy) are widespread in Streptomyces , key enzyme families are enriched in highly cellulolytic strains. (osti.gov)
  • Linking secondary metabolites to biosynthesis genes in the fungal endophyte Cyanodermella asteris: The anti-cancer bisanthraquinone skyrin. (uni-bielefeld.de)
  • Note that these mutations caused abnormal type 1 and type 17 helper T-cell responses, which have been linked to susceptibility to fungal infections. (medpagetoday.com)
  • The present study evaluated the associations between Dectin-1 and DC-SIGN gene single nucleotide polymorphisms (SNPs) and susceptibility to fungal keratitis (FK) in the northern Han Chinese population. (molvis.org)
  • These are enigmatic molecules whose action requires the presence of plant genes with products that specifically encode sensitivity to the toxin and susceptibility to the disease. (edu.au)
  • The SNP frequencies of the B (the variant or minor allele) and A (the wild or major allele) alleles of the dectin-1 gene in both cases and controls were analyzed with regard to FI susceptibility. (cdc.gov)
  • They were confident that the genetic defect lay in an immune-related pathway and therefore focused their mutation search on 100 genes related to immune function, including STAT1 . (medpagetoday.com)
  • Although, C . albicans shows a predominantly clonal population structure, we find evidence of gene flow between previously known and newly identified genetic clusters, supporting the occurrence of (para)sexuality in nature. (nature.com)
  • It can reveal allopatric differentiation, host adaptation or other types of local adaptation as consequences of reduction of gene flow promoting genetic drift and natural selection 1 . (nature.com)
  • Were new genes added by the Creator, or was pre-existing genetic information corrupted, deleted, or altered in some way? (icr.org)
  • As we discover more of these single-gene, inborn immune system defects, we can give susceptible individuals with a family history of the disease a chance to prevent it, through molecular diagnosis and genetic counseling, as well as targeted treatments that restore a poor immune response ," says Casanova, who is senior attending physician at the Rockefeller University Hospital. (medicalxpress.com)
  • However, its genetic basis remains unclear, especially in the context of gene contractions of conventional carbohydrate-active enzymes (CAZYs) relative to white rot ancestors. (asm.org)
  • Recently, genetic engineering of biocontrol agents with reporter or marker genes has provided useful tools for detection and monitoring of introduced biocontrol agents in natural environments ( 14 , 21 ). (asm.org)
  • Genetic and in silico analyses led to the identification of a gene cluster of five genes suggested to encode the enzymatic pathway for skyrin. (uni-bielefeld.de)
  • Unfortunately, the genetic tractability of A. fumigatus has lagged behind some other fungal systems, particularly in the area of conditional expression systems. (biomedcentral.com)
  • The research is funded by the Grains Research and Development Corporation's (GRDC) Effective Genetic Control of SNB project, with wheat gene and DNA marker analysis from the State Agricultural Biotechnology Centre at Murdoch University. (phys.org)
  • Compared to traditional methods of fungal exposure assessment, molecular methods have provided new insight into the richness of fungal communities present in both indoor and outdoor environments. (cdc.gov)
  • Although molecular diagnosis is often based on the detection of mtLSU rRNA mitochondrial gene, the number of copies of mitochondrial genes had not been investigated. (frontiersin.org)
  • The Institute of Molecular Plant Sciences currently consists of 11 research groups working in four overlapping areas of plant and fungal biology. (ed.ac.uk)
  • Based on predicted amino acid sequences, functions for two of these three genes are hypothesized. (nih.gov)
  • They can be fused to regulatory sequences or genes of interest to report expression location or levels. (nature.com)
  • Transcripts were apparent for some gene copies, but, in most cases, the endogenous sequences lacked the residues necessary for normal viral functioning. (blogspot.com)
  • Change in Gibbs free energy (ΔG) of the best pattern promoter resulting from the interaction between RNA polymerase and the promoter sequences of modified genes of phytase was equal to -9 kcalmol-1, which is lower compared to other interactions. (ac.ir)
  • To attain highquality gene models, this program runs multiple gene predictors, evaluates all predicted genes, and assembles gene models that are highly supported by homology to known sequences. (elsevier.com)
  • Recently, the Aspergillus terreus terrein gene cluster was identified and selected for development of a new heterologous expression system. (nottingham.ac.uk)
  • Additionally, expression of the Aspergillus nidulans polyketide synthase gene orsA revealed lecanoric acid rather than orsellinic acid as major polyketide synthase product. (nottingham.ac.uk)
  • We compared the gene expression stimulated with fungal extracts from Aspergillus (A.) fumigatus, Alternaria (A.) alternata, or Penicillium (P.) notatum in NCI-H292 (a human bronchial epithelial cell line) to search Allergic bronchopulmonary mycosis (ABPM)-related genes. (omicsdi.org)
  • In the current study, we functionally analysed these three catalase-peroxidase genes and found that MgCatD-1, encoding a secreted catalase-peroxidase, plays an important role in the pathogenicity of Z. tritici. (wur.nl)
  • These pea pathogenicity (PEP) genes are within 25 kb of each other and are located on a supernumerary chromosome. (nih.gov)
  • This strategy was successful for the cloning of pathogenicity genes in Colletotrichum lindemuthianum ( 7 ), Ustilago maydis, ** and M. grisea ( 8 , 9 ). (pnas.org)
  • 2. We study gene-regulatory programs that control microbial pathogenicity. (edu.au)
  • Madhu studies innate immune interactions of fungal pathogenicity. (edu.au)
  • Host‐induced gene silencing of an important pathogenicity factor PsCPK1 in Puccinia striiformis f. sp. (semanticscholar.org)
  • Plants are transformed with a silencing construct homologous to a gene of a plant pest that is essential for the survival, development, or pathogenicity of the pest. (patentsencyclopedia.com)
  • 8. The method of claim 1, wherein the first pest pathogenicity gene is from a Phytophthora. (patentsencyclopedia.com)
  • and wherein the second pathogenicity gene is from an aphid. (patentsencyclopedia.com)
  • 9. The method of claim 1, wherein the first pest pathogenicity gene is from Phytophthora sojae and wherein the second pathogenicity gene is from Phakospora pachyrhizi. (patentsencyclopedia.com)
  • Our studies monitored expression profiles of 14 defense related genes in 11 rice breeding lines derived from an elite cultivar with different combination of R genes against BB, BL and GM under single and multiple challenge. (springer.com)
  • A high-throughput gene-silencing system for the functional assessment of defense-related genes in barley epidermal cells. (semanticscholar.org)
  • Several promoter regions of the trpC and crp genes were fused to the npgA gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in A. nidulans to evaluate color recovery due to the transcriptional activity of the sequence elements. (bvsalud.org)
  • Expression of eight of the genes was co-ordinately induced under cercosporin-producing conditions and was regulated by the Zn(II)Cys 6 transcriptional activator, CTB8. (wiley.com)
  • Similar 'feedback' transcriptional inhibition was observed when the CTB1 , or CTB3 but not CTB4 gene was inactivated. (wiley.com)
  • Using targeted gene deletions, we verify the importance of a highly expressed cellulase (GH6 family cellobiohydrolase) and the CebR transcriptional repressor to the cellulolytic phenotype. (osti.gov)
  • Evolutionary analyses identify complex genomic modifications that drive plant biomass deconstruction in Streptomyces , including acquisition and selective retention of CAZy genes and transcriptional regulators. (osti.gov)
  • Characterization of fungal two-component signaling is relatively limited. (asm.org)
  • Collectively, these results support the hypothesis that gene regulation shifts played a pivotal role in brown rot adaptation. (asm.org)
  • To model an essential gene under tetracycline regulation, the E. coli hygromycin resistance gene, hph , was placed under the control of seven copies of the TetR binding site ( tetO 7 ) in a plasmid vector and co-transfected into A. fumigatus protoplasts together with one of the two transactivator plasmids. (biomedcentral.com)
  • The levels of doxycycline required to regulate expression of the tetO 7 - hph reporter gene were within non-toxic ranges for this organism, and low-iron medium was shown to reduce the amount of doxycycline required to accomplish regulation. (biomedcentral.com)
  • This suggests that apoplastic phytopathogens may react to similar physiological parameters for regulation of their effector genes. (semanticscholar.org)
  • These results indicate the existence of a complex catalase system in C. fulvum, with regard to both the structure and regulation of the genes involved. (edu.au)
  • However, no detailed study has been made on possible interactions among these genes when expressed simultaneously under combined stresses. (springer.com)
  • Understanding the function of M. oryzae effectors, their host targets, and AVR-R gene interactions is important to devise effective means to control the disease. (plantcell.org)
  • The first burst in gene expression correlated with appressorium formation and penetration of epidermal cells, while the second burst of gene expression changes followed the onset of haustoria formation in both compatible and incompatible interactions. (plantphysiol.org)
  • The temporal relationships between P. pachyrhizi growth and host responses provide an important context in which to view interacting gene networks that mediate the outcomes of their interactions. (plantphysiol.org)
  • Role of Fungal Endosymbionts in Host Reproduction This project focuses on endosymbionts and fungal reproduction, expanding knowledge on the complexities of microbiome interactions. (doe.gov)
  • These results suggest complex interactions between gene expression ( TRI genes), environmental factors and mycotoxin production. (royalsocietypublishing.org)
  • Fungal effector-host sensitivity gene interactions play a key role in determining the outcome of septoria nodorum blotch disease (SNB) caused by Parastagonospora nodorum on wheat. (edu.au)
  • Furthermore, we found that the Y. lipolytica PEX9 gene, a putative orphan gene, might encode a Pex26p ortholog. (nih.gov)
  • Sequence analysis identified 10 putative open reading frames (ORFs) flanking the previously characterized CTB1 and CTB3 genes that encode, respectively, the polyketide synthase and a dual methyltransferase/monooxygenase required for cercosporin production. (wiley.com)
  • The predicted pathway for the synthesis of viriditoxin was established by a combination of genomics, bioinformatics, gene disruption and chemical analysis processes. (biomedcentral.com)
  • Association analyses of these 316 genes revealed three novel AVR genes, AVR-Pia , AVR-Pii , and AVR-Pik/km/kp , corresponding to five previously known AVR genes, whose products are recognized inside rice cells possessing the cognate R genes. (plantcell.org)
  • However, single-gene analyses are dependent on the gene having an evolutionary history that reflects that of the entire organism, an assumption that is not always true. (biomedcentral.com)
  • The expression data for six key transcription genes ( TRI4, TRI5 , TRI6, TRI10, TRI12 and TRI13 ) were analysed using multiple regression analyses to model the relationship between these various factors for the first time. (royalsocietypublishing.org)
  • This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies. (bvsalud.org)
  • Host gene expression analyses also identified interesting sets of genes up-regulated, including those involving stress response, host defense response, and several agamous-like MADS-box genes (AGL61 and AGL80), predicted to interact and be involved in male gametophyte development. (g3journal.org)
  • Our analysis predicts that all Pezizomycotina unlike Saccharomycotina can potentially produce a wide variety of secondary metabolites and secreted enzymes and that the responsible gene families are likely to evolve fast. (biomedcentral.com)
  • Other genes not essential for β-lactam production are downregulated with a preference for those responsible for transport processes or biosynthesis of other secondary metabolites. (biomedcentral.com)
  • As it turns out, 42% of the missing genes were involved in amino acid and purine metabolism-standard biochemical pathways that are built-in to most creatures. (icr.org)
  • A portion of a key gene involved in ethylbenzene metabolism (ElHDO - homogentisate dioxygenase) was isolated and its gene expression monitored as a function of substrate feed, nutrient concentration and transient loading conditions. (utexas.edu)
  • Therefore further studies and exploitation of fungal metabolism appears very promising. (biomedcentral.com)
  • The major finding of our comparative transcriptome analysis is that strain improvement programs in two unrelated fungal β-lactam antibiotic producers alter the expression of target genes of Velvet, a global regulator of secondary metabolism. (biomedcentral.com)
  • β-lactam antibiotics represent a large fraction of these drugs, and are the product of bacterial or fungal secondary metabolism. (biomedcentral.com)
  • article{osti_1379648, title = {Identification of Glutaminyl Cyclase Genes Involved in Pyroglutamate Modification of Fungal Lignocellulolytic Enzymes}, author = {Wu, Vincent W. and Dana, Craig M. and Iavarone, Anthony T. and Clark, Douglas S. and Glass, N. Louise}, abstractNote = {The breakdown of plant biomass to simple sugars is essential for the production of second-generation biofuels and high-value bioproducts. (osti.gov)
  • The biosynthetic pathways of fungal aromatic polyketides usually involve a set of successive enzymes, in which a non-reductive polyketide synthase iteratively catalyzes the essential assembly of simple building blocks into (often polycyclic) aromatic compounds. (uni-bielefeld.de)
  • These enzymes are responsible for the breakdown of chitin and glucan, two major components of the fungal cell walls. (edu.au)
  • In P. chrysogenum , 748 genes showed differential expression, while 1572 genes from A. chrysogenum are differentially expressed in the industrial strain. (biomedcentral.com)
  • Remarkably, a total of 1.68 Mb regions, comprising 316 candidate effector genes, were present in Ina168 but absent in the assembled sequence of isolate 70-15. (plantcell.org)
  • Impact of biotic and abiotic factors on the expression of fungal effector-encoding genes in axenic growth conditions. (semanticscholar.org)
  • Differential effector gene expression underpins epistasis in a plant fungal disease. (edu.au)
  • The pathosystem is complex and mediated by interaction of multiple fungal necrotrophic effector-host sensitivity gene systems. (edu.au)
  • Internal transcribed spacer rRNA gene sequencing analysis of fungal diversity in Kansas City indoor environments. (cdc.gov)
  • PCR was performed with primer pairs targeted to the 18S rRNA gene. (biomedcentral.com)
  • The PCR positive samples were identified by DNA sequencing of the internal transcribed spacer (ITS) region of the rRNA gene. (biomedcentral.com)
  • The 28S rRNA gene PCR evaluation was carried out by Anand et al. (biomedcentral.com)
  • The aim of this study is to evaluate a PCR method based on a new set of primers targeted to 18S rRNA gene for rapid detection of pan-fungal aetiology in microbial keratitis. (biomedcentral.com)
  • To correlate soybean responses with P. pachyrhizi growth and development, we inoculated the soybean cultivar Ankur (accession PI462312 ), which carries the Rpp3 resistance gene, with avirulent and virulent isolates of P. pachyrhizi . (plantphysiol.org)
  • Carriers of the haplotype TC (rs4804803 and rs2287886) had a higher risk of developing fungal keratitis (p = 0.007, OR = 1.710, 95% CI 1.154-2.534). (molvis.org)
  • Fungal keratitis (FK) is recognized as one of most serious vision-threatening corneal infectious diseases because it can result in endophthalmitis, corneal perforation, and vision loss. (molvis.org)
  • The sensitivity and specificity of 18S rRNA polymerase chain reaction (PCR) in the detection of fungal aetiology of microbial keratitis was determined in thirty patients with clinical diagnosis of microbial keratitis. (biomedcentral.com)
  • Statistical analysis revealed that the PCR to have a sensitivity of 90.9% and specificity of 94.7% in the detection of a fungal aetiology in microbial keratitis. (biomedcentral.com)
  • PCR is a rapid, sensitive and useful method to detect fungal aetiology in microbial keratitis. (biomedcentral.com)
  • Its diagnosis remains a difficult problem, although the history and clinical appearance may suggest fungal keratitis. (biomedcentral.com)
  • Gram stain and culture techniques occasionally fail in the detection of fungal keratitis. (biomedcentral.com)
  • Our results have significant implications for strategies using the gene combination Avr9/Cf-9 to engineer plants with enhanced disease resistance. (pnas.org)
  • Study of fungal prions has led to a characterisation of the sequence features and mechanisms that enable prion domains to switch between functional and amyloid-forming states. (wikipedia.org)
  • Analyzing 14 patients from five families affected with CMC, Dutch and British researchers found missense mutations in the STAT1 gene, an important player in immune function, according to an online report in the New England Journal of Medicine . (medpagetoday.com)
  • The researchers focused on a particular gene, CARD9, because previous studies of other fungal infections had found a defect on the gene to be the cause. (medicalxpress.com)
  • By studying their DNA the researchers found that there was a common recurrent mutation in a gene called CARD9. (brighthub.com)
  • Over the past few years, the researchers have devised various nanonutrient concoctions that boost the fungal resistance of soybeans, tomatoes , watermelons and, recently, eggplants , as reported in the April Plant Disease . (sciencenews.org)
  • Researchers have identified a gene that promotes metastases, the spread of cancer cells through the body. (scienceblog.com)
  • Washington, DC?Researchers who are now at Georgetown University's Lombardi Cancer Center have identified a gene that promotes metastases, the spread of cancer cells through the body. (scienceblog.com)
  • Researchers at the Francis Crick Institute have found two genes that regulate the differentiation of stem cells in the small intestine, offering valuable insight into how the body develops and maintains a healthy gut. (news-medical.net)
  • The deduced amino acid sequence of another transcribed portion of the PEP cluster, as well as four other open reading frames in the cluster, have a high degree of similarity to known fungal transposases. (nih.gov)
  • We describe a core gene cluster, comprised of eight genes (designated CTB1-8 ), and associated with cercosporin toxin production in Cercospora nicotianae . (wiley.com)
  • Disruption of the CTB2 gene encoding a methyltransferase or the CTB8 gene yielded mutants that were completely defective in cercosporin production and inhibitory expression of the other CTB cluster genes. (wiley.com)
  • The function of the eight-membered gene cluster of P. variotii was characterized by targeted gene disruptions, revealing the roles of each gene in the synthesis of this molecule and establishing its biosynthetic pathway, which includes a Baeyer-Villiger monooxygenase catalyzed reaction. (biomedcentral.com)
  • But in very rare cases, fungal infections can spread below the skin's surface and onto the lymph nodes, bones, digestive tract or even the brain. (medicalxpress.com)
  • What Makes People Get Fungal Infections? (brighthub.com)
  • There are many different types of unpleasant fungal infections that afflict humans. (brighthub.com)
  • Why do People Get Fungal Infections? (brighthub.com)
  • These people had been afflicted by recurrent fungal infections, which proved to be fatal in some cases. (brighthub.com)
  • Further proof of this was obtained from animal models where mice lacking either of these genes were susceptible to fungal infections. (brighthub.com)
  • Mice response to fungal infections had previously been studied and known, and the mechanisms are the same in humans. (brighthub.com)
  • Most recently, Anna Savage and Kelly Zamudio took a big step by taking measurements of positive selection for immunity out of the laboratory and into the field by analyzing MHC genes in wild populations of frogs with ongoing chytrid infections . (molecularecologist.com)
  • Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties. (sigmaaldrich.com)
  • The most vulnerable patients, such as those affected by cancer or HIV, are particularly susceptible to life-threatening fungal diseases. (edu.au)
  • This penetration process is typical of fungal leaf spots such as rice blast caused by the filamentous ascomycete Magnaporthe grisea ( 3 ). (pnas.org)
  • However, in filamentous ascomycetes genes were found to be conserved within homologous chromosomes, but with randomized orders and orientations, a phenomenon named mesosynteny. (wikipedia.org)
  • Hierarchical clustering analysis (HCA) allowed us to define five major clusters, significantly associated with fungal load ( p = 0.029), in which copy number of mitochondrial genes was significantly different among them. (frontiersin.org)
  • Recent fungal diseases of crop plants: Is lateral gene transfer a common theme? (edu.au)
  • It is also notable that many new diseases belong to the fungal taxon dothideomycetes. (edu.au)
  • This review explores the coincidence of new diseases, interspecific gene transfer, host-specific toxins, and the dothideomycete class. (edu.au)
  • Chances are, most - if not all - of the produce in your kitchen is threatened by fungal diseases. (sciencenews.org)
  • Annually, fungal diseases destroy a third of all harvests and pose a dire threat to global food security. (sciencenews.org)
  • To stop the spread of fungal diseases, farmers fumigate the soil with toxic chemicals that lay waste to the land, sparing not even the beneficial microbes teeming in the earth. (sciencenews.org)
  • Knowing those genes will help better understanding the mechanisms behind necrothrophic tree diseases and developing new control strategies. (wsl.ch)
  • Without intense research and scientific study, catastrophic harvest failures due to fungal diseases could cause food shortages, human and animal poisonings, and economic loss throughout the world. (routledge.com)
  • 60%) of conventional lignocellulolytic genes, implying that ROS adaptations supplanted conventional pathways. (asm.org)
  • To identify host genes involved in the resistance response, expression profiles of the wheat line Tadinia (containing the Stb4 gene for resistance) and the susceptible line Yecora Rojo, non-inoculated or inoculated with M. graminicola, were compared by differential-display polymerase chain reaction (DD-PCR). (deepdyve.com)
  • In contrast, a wheat lipoxygenase was down-regulated in the resistant lines at time points corresponding with peak induction of the PR genes. (deepdyve.com)
  • Host-Induced Gene Silencing of the MAPKK Gene PsFUZ7 Confers Stable Resistance to Wheat Stripe Rust. (semanticscholar.org)
  • Recently, evidence was obtained that wheat tan spot emerged through a lateral gene transfer event some time prior to 1941. (edu.au)
  • Research is underway that will pave the way for new, improved wheat varieties that are more resistant to a significant fungal disease that costs Western Australian growers $108 million each year. (phys.org)
  • Department of Agriculture and Food is using new genomic technology from its counterparts in Victoria to identify and track gene combinations that produce resistance to SNB, for future use in breeding new commercial wheat varieties . (phys.org)
  • It is quite a complex process, as it is the accumulated effect of several genes that creates resistance to the fungal disease, but we have now targeted the genes to deploy into breeding wheat suitable for WA growing regions. (phys.org)
  • We report here the effects of expressing the Cladosporium fulvum avirulence Avr9 gene product in a tomato line containing the Cf-9 disease-resistance gene. (pnas.org)
  • These fungal resistant parents were crossed with other varieties that have desirable fruit qualities in an effort to combine fungal disease resistance with desirable fruit qualities in a single variety. (sun.ac.za)
  • In this study, Apolipoprotein E (ApoE) polymorphism and lipoprotein cholesterol concentrations were compared between 42 patients with superficial fungal disease and 27 control subjects. (medeniyet.edu.tr)
  • The patients with superficial fungal disease bad significantly higher concentrations of high-density cholesterol (HDL) compared to the control group (p=0.0462). (medeniyet.edu.tr)
  • It was concluded that the presence of apoE2/3 genotype, high HDL-cholesterol levels and the absence of apoE3/3 genotype can be regarded as risk factors for superficial fungal disease, especially dermatophytosis. (medeniyet.edu.tr)
  • Fungal prions have provided a model for the understanding of disease-forming mammalian prions. (wikipedia.org)
  • Most eukaryotic and all fungal HKs are hybrids. (asm.org)
  • In the present study, a fungal nitrilase was cloned from the cDNA sequence of G. intermedia and successfully expressed in E. coli Rosetta-gami (DE3). (sigmaaldrich.com)
  • In the latter, the organization is identical in that both genes share a common promoter sequence. (biomedcentral.com)
  • Prevalence of individual fungal phyla determined in the ITS sequence analysis of KCSHHP air and dust samples. (cdc.gov)