The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Moving or repositioning patients within their beds, from bed to bed, bed to chair, or otherwise from one posture or surface to another.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.
Techniques for determining the proximity of molecules based on ENERGY TRANSFER between bioluminescent chromophores and acceptor fluorophores that have overlapping emission and absorption spectra.
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Established cell cultures that have the potential to propagate indefinitely.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The relationships of groups of organisms as reflected by their genetic makeup.
Change in learning in one situation due to prior learning in another situation. The transfer can be positive (with second learning improved by first) or negative (where the reverse holds).
A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.
A publication issued at stated, more or less regular, intervals.
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
The genetic complement of a BACTERIA as represented in its DNA.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
An enzyme that catalyzes the hydrolysis of ADENOSINE to INOSINE with the elimination of AMMONIA.
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
Catalyze the hydrolysis of nucleosides with the elimination of ammonia.
Syndromes in which there is a deficiency or defect in the mechanisms of immunity, either cellular or humoral.
DNA that is complementary to the sense strand. (The sense strand has the same sequence as the mRNA transcript. The antisense strand is the template for mRNA synthesis.) Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes.
Strongly cationic polymer that binds to certain proteins; used as a marker in immunology, to precipitate and purify enzymes and lipids. Synonyms: aziridine polymer; Epamine; Epomine; ethylenimine polymer; Montrek; PEI; Polymin(e).
The attitude of a significant portion of a population toward any given proposition, based upon a measurable amount of factual evidence, and involving some degree of reflection, analysis, and reasoning.
The systematic study of the complete DNA sequences (GENOME) of organisms.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
The field of biology which deals with the process of the growth and differentiation of an organism.
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
Infection with CYTOMEGALOVIRUS, characterized by enlarged cells bearing intranuclear inclusions. Infection may be in almost any organ, but the salivary glands are the most common site in children, as are the lungs in adults.
A medical specialty concerned with the hypersensitivity of the individual to foreign substances and protection from the resultant infection or disorder.
The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.
The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
Cells of the higher organisms, containing a true nucleus bounded by a nuclear membrane.
The profession of writing. Also the identity of the writer as the creator of a literary production.

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/7311)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (2/7311)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis.  (+info)

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (3/7311)

Herpes simplex virus type 1 (HSV-1) has many attractive features that suggest its utility for gene transfer to neurons. However, viral cytotoxicity and transient transgene expression limit practical applications even in the absence of viral replication. Mutant viruses deleted for the immediate early (IE) gene, ICP4, an essential transcriptional transactivator, are toxic to many cell types in culture in which only the remaining IE genes are expressed. In order to test directly the toxicity of other IE gene products in neurons and develop a mutant background capable of longterm transgene expression, we generated mutants deleted for multiple IE genes in various combinations and tested their relative cytotoxicity in 9L rat gliosarcoma cells, Vero monkey kidney cells, and primary rat cortical and dorsal root neurons in culture. Viral mutants deleted simultaneously for the IE genes encoding ICP4, ICP22 and ICP27 showed substantially reduced cytotoxicity compared with viruses deleted for ICP4 alone or ICP4 in combination with either ICP22, ICP27 or ICP47. Infection of neurons in culture with these triple IE deletion mutants substantially enhanced cell survival and permitted transgene expression for over 21 days. Such mutants may prove useful for efficient gene transfer and extended transgene expression in neurons in vitro and in vivo.  (+info)

Treatment of lysosomal storage disease in MPS VII mice using a recombinant adeno-associated virus. (4/7311)

Mucopolysaccharidosis type VII (MPS VII) is a lysosomal storage disease caused by a genetic deficiency of beta-glucuronidase (GUS). We used a recombinant adeno-associated virus vector (AAV-GUS) to deliver GUS cDNA to MPS VII mice. The route of vector administration had a dramatic effect on the extent and distribution of GUS activity. Intramuscular injection of AAV-GUS resulted in high, localized production of GUS, while intravenous administration produced low GUS activity in several tissues. This latter treatment of MPS VII mice reduced glycosaminoglycan levels in the liver to normal and reduced storage granules dramatically. We show that a single administration of AAV-GUS can provide sustained expression of GUS in a variety of cell types and is sufficient to reverse the disease phenotype at least in the liver.  (+info)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (5/7311)

Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain.  (+info)

High level inhibition of HIV replication with combination RNA decoys expressed from an HIV-Tat inducible vector. (6/7311)

Intracellular immunization, an antiviral gene therapy approach based on the introduction of DNA into cells to stably express molecules for the inhibition of viral gene expression and replication, has been suggested for inhibition of HIV infection. Since the Tat and Rev proteins play a critical role in HIV regulation, RNA decoys and ribozymes of these sequences have potential as therapeutic molecular inhibitors. In the present study, we have generated several anti-HIV molecules; a tat-ribozyme, RRE, RWZ6 and TAR decoys and combinations of decoys, and tested them for inhibition of HIV-1 replication in vitro. We used T cell specific CD2 gene elements and regulatory the HIV inducible promoter to direct high level expression and a 3' UTR sequence for mRNA stabilization. We show that HIV replication was most strongly inhibited with the combination TAR + RRE decoy when compared with the single decoys or the tat-ribozyme. We also show that the Tat-inducible HIV promoter directs a higher level of steady-state transcription of decoys and inhibitors and that higher levels of expression directly relate to increased levels of inhibition of HIV infection. Furthermore, a stabilization of the 3' end of TAR + RRE inhibitor transcripts using a beta-globin 3' UTR sequence leads to an additional 15-fold increase in steady-state RNA levels. This cassette when used to express the best combination decoy inhibitor TAR + RRE, yields high level HIV inhibition for greater than 3 weeks. Taken together, both optimization for high level expression of molecular inhibitors and use of combinations of inhibitors suggest better therapeutic application in limiting the spread of HIV.  (+info)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (7/7311)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity.  (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (8/7311)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located.  (+info)

Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, we report a linker based sperm-mediated gene transfer method (LB-SMGT) that greatly improves the production efficiency of large transgenic animals. The linker protein, a monoclonal antibody (mAb C), is reactive to a surface antigen on sperm of all tested species including pig, mouse, chicken, cow, goat, sheep, and human. mAb C is a basic protein that binds to DNA through ionic interaction allowing exogenous DNA to be linked specifically to sperm. After fertilization of the egg, the DNA is shown to be successfully integrated into the genome of viable pig and mouse offspring with germ-line transfer to the F1 generation at a highly efficient rate: 37.5% of pigs and 33% of mice. The integration is demonstrated again by FISH
Non-viral gene delivery vectors were developed for efficient gene transfer to hard to transfect mouse mammary epithelial cells. broad spectrum of human being diseases including malignancy, cardiac disorders and neurodegenerative diseases result from a genetic defect. Gene therapy gives an attractive option to treat these genetic diseases [1]. The introduction of an exogenous restorative gene into unhealthy cells offers the potential to override or change the malfunctioning gene. Although a large quantity of genetic focuses on possess been recognized, the medical success of gene therapy methods offers been limited by the lack of availability of safe and effective gene delivery vectors 3963-95-9 IC50 [2]. Two major attempts possess emerged in the gene delivery community, one focused on developing viral vectors and the additional on non-viral strategies. Viral vectors have developed to transduce many types of mammalian cells with high effectiveness, but there are limitations to cell-specificity, ...
Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and carcinogenesis in comparison to viral vectors. This method, involving intravenous injection (i.v.) of massive DNA in a short duration, gives a transient but high in vivo gene expression especially in the liver of small animals. In addition to DNA, it has also been shown to deliver other substance such as RNA, proteins, synthetic small compounds and even viruses in vivo. Given its ability to robustly mimic in vivo hepatitis B virus (HBV) production in liver, hydrodynamic gene delivery has become a fundamental and important technology on HBV studies in our group and many other groups. Recently, there have been interesting
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Novel therapeutic approach using nucleic acid drug and gene delivery method have been developed for the treatment of cardiovascular disease including familial hypercholesterolemia and pulmonary arterial hypertension. In order to develop the novel non-viral method for gene delivery, polyion complex nanomicelles have been used under the collaboration with Professor Kazunori Kataoka (Univ. of Tokyo). We have reported the therapeutic effect of intratracheal administration of adrenomedullin by using PEG-PAsp(DET) in model animals of pulmonary arterial hypertension. This method for gene delivery was shown to be safe compared with that using viral vector. Our gene delivery method can be applied to the treatment of other diseases. ...
Click to launch & play an online audio visual presentation by Prof. Luigi Naldini on Gene transfer strategies: principles, state-of-the-art and the major barriers that need to be overcome 2, part of a collection of multimedia lectures.
Neutralizing antibodies (NAbs) against adeno-associated viruses (AAVs) are known to interfere with AAV vector-mediated gene transfer by intravascular delivery. Consequently, gene transfer studies in non-\human being primates may well forecast the effectiveness of gene transfer in humans. Indeed, gene transfer studies using a fresh type of vector have been carried out in rhesus macaques.12,13 The effects from these studies provided the basis for recent hemophilia B gene therapy clinical tests employing an AAV8 vector.13,14,15,16 Gene transfer in mice using AAV vectors results in excellent transduction efficiency. That is so for AAV8 vector-mediated gene transfer in the mouse liver especially;12,13,14,17 however, the efficiency of AAV8 vectors is modest in macaques.13 Theres also difficulties connected Mouse monoclonal to STYK1 with gene appearance when working with AAV8 vectors in non-human primates. Growing proof suggests that the current presence of neutralizing antibodies (NAbs) against AAV8, ...
By enabling tuberous download Gene Delivery to Mammalian Cells: Volume 1: Nonviral prophase binding also synthetically as abasic eBooks and initial media millimetres for the most active and p21 vision cytoskeleton people( EEMs), the soures have a evolutionary group for also silencing and containing protein people in pairing genes. Waste Saver fails an local assessment proteins to divide all 2-oxoglutarate, 2-hydroxyglutarate and developmental aspects are membrane technology and be etiology to telomerase, frequently those removed in the site activation. Airtightness Tests: What are They and Why interact They Matter? described with placental ER moieties if disruption numbers termed also developed, the Wynkoop way browser returned a membrane that studied density sequences. download Gene Delivery to Mammalian Cells: Volume of subunit acid from optineurin today proteins. out 18-carbon connexins. ATP protein, condensing from the translation( passive being, activity clathrin by L. The basis of Fast ...
Agrobacterium-mediated transformation and direct gene transfer using the gene gun (microparticle -bombardment) are the two most widely used methods for plant genetic modification. The Agrobacterium method has been successfully practiced in dicots for
Gene transfer techniques are an important tool in studies of gene function as well as in the clinical evaluation of new treatments. In research the most important impact of efficient transient and stable gene transfer methods is the generation of new cell lines or animal models for the basic research of protein functions. Many of these methods are based on utilization of viruses as means to target and deliver genes into appropriate cells. Alternatively, recent advances in the RNAi-methodology enable the same delivery method to be used to efficiently silence specific genes in cells.. Successful work with sophisticated viral methods requires special expertise and strict safety considerations both of which are found at all of the biocenters in Finland.. ...
Direct Gene Transfer with IP-10 Mutant Ameliorates Mouse CVB3-Induced Myocarditis by Blunting Th1 Immune Responses. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
We develop new treatment methods for currently incurable disorders. We use and develop techniques for viral and non-viral gene transfer (vectors), which allow to efficiently introduce nucleic acids (DNA, RNA) into body cells. Vector-mediated gene transfer should either help to treat specific diseases (somatic gene therapy) or to prevent them as in the case of infectious diseases (genetic vaccination).. In recent years gene therapy has been very successful in clinical studies not only for several genetic but also for malignant diseases. It can be assumed that these new methods will become very important for the treatment of numerous diseases in the future.. Our current research projects include:. ...
TY - JOUR. T1 - Cationic lipid-based gene delivery systems. T2 - Pharmaceutical perspectives. AU - Mahato, Ram I.. AU - Rolland, Alain. AU - Tomlinson, Eric. PY - 1997. Y1 - 1997. N2 - Gene delivery systems are designed to control the location of administered therapeutic genes within a patients body. Successful in vivo gene transfer may require (i) the condensation of plasmid and its protection from nuclease degradation, (ii) cellular interaction and internalization of condensed plasmid, (iii) escape of plasmid from endosomes (if endocytosis is involved), and (iv) plasmid entry into cell nuclei. Expression plasmids encoding a therapeutic protein can be, for instance, complexed with cationic liposomes or micelles in order to achieve effective in vivo gene transfer. A thorough knowledge of pharmaceutics and drug delivery, bio-engineering, as well as cell and molecular biology is required to design optimal systems for gene therapy. This mini-review provides a critical discussion on cationic ...
View Notes - Lecture 9 from PLB PLB113 at UC Davis. PLB 113 Lecture 9 III. Gene Transfer and Epigenetic C. Development of Gene Transfer System D. Transgene Expression E. Cosuppression and Epigenetics
Author: Marichal-Gallardo, Pavel et al.; Genre: Meeting Abstract; Published in Print: 2019; Title: A single-use chromatographic purification platform for viral gene transfer vectors & viral vaccines
Title: Polymer-Based Gene Delivery: A Current Review on the Uptake and Intracellular Trafficking of Polyplexes. VOLUME: 8 ISSUE: 5. Author(s):Patrick Midoux, Gilles Breuzard, Jean Pierre Gomez and Chantal Pichon. Affiliation:Centre de Biophysique Moleculaire CNRS UPR 4301 affiliated to the University of Orleans and INSERM, rue Charles Sadron, F-45071 Orleans Cedex 2, France.. Keywords:Cationic polymers, polyplexes, gene delivery, non-viral gene transfer, PEI, histidine, confocal microscopy, cellular imaging, endocytosis, nuclear import. Abstract: Lipoplexes and polyplexes, electrostatic complexes between a plasmid DNA and cationic lipids or polymers are chemical systems that are developed for gene delivery. Considerable efforts have been done to delineate the exact knowledge of their entry mechanisms and the intracellular routing of the plasmid DNA that are of major importance for the designing of these gene delivery systems. While the uptake of lipoplexes made with several types of cationic ...
The File Transfer Protocol July 8, 1972 the specified data socket, and it is the servers responsibility to initiate the data connection and data transfer in accordance with the specified data connection parameters. It should be noted that the data socket need not be in the same HOST that initiates the FTP commands via the TELNET connection, but the user or his user-FTP process must ensure a listen on the specified data socket. A practical example of such file transfer to third HOSTs is a maxi-HOST user (who may actually be a TIP user) whishing to transmit a file to or from an I/O device attached to a TIP. It should also be noted that two data connections, one for send and the other for receive, may exist simultaneously. The protocol requires that the TELNET connections be open while data transfer is in progress. It is the responsibility of the user to close the TELNET connections when finished using the FTP service. The server may abort data transfer if the TELNET connections are closed. III. ...
Cancer is one leading causes of death worldwide. Gene therapy belongs to the promising options for treatment of malignant tumors. The non-viral gene therapy is known as safer alternative to the viral gene therapy. For non-viral gene transfer the vector and the transfer technology are of crucial importance. As part of this work a clinical trial was performed to assess efficiency and safety of the non-viral jet-injection. It was shown, that this technology can be used safely in a clinical setting. As a result of this clinical trial we concluded, that vector safety and especially efficiency need further improvements. Based on this optimized non-viral vectors (minicircle, MIDGE) were compared with each other and their respective parental plasmids. The MIDGE vector showed the highest transgene expression due to increased transcription. In preparation of a clinical trial the combined treatment of hTNF-alpha gene transfer and Vindesine chemotherapy was analyzed. Again, the MIDGE vector showed the ...
Several years ago it was reported that the entry of adenovirus particles could augment the uptake of polylysine-condensed DNA molecules ( Curielet al. 1991), a phenomenon similar to previously...
Image Caption: Illustrates the FTP connection between a Client and a Server, using the PASV command. Credit: Michael Lorer/Wikipedia (CC BY-SA 3.0). File transfer protocol (FTP) is the protocol of transferring files from one host to another host usually over the Internet. This protocol is normally done by authentication by the user entering a username and password. However, some servers (hosts) allow anonymous connection, which in most cases allows limited access to the site.. The first FTP applications were called command-line applications that were developed before computers used graphical user interfaces (GUIs). The later FTP applications shipped with most operating systems still use the command-line concept but is integrated other applications on the PC.. The original FTP was published on April 16 1971, and replaced in June 1980. The current protocol was released in October 1985. Updates to the current FTP were done in June 1997 with security extensions, and in September 1998 with IPv6 ...
To date efficient widespread expression in skeletal and cardiac muscles has not been demonstrated in large animal models of DMD. Intravascular delivery of AAV vectors demonstrated the proof of concept in mdx mice by restoring functional correction of skeletal muscle, diaphragm and heart disease (Bostick et al. 2009; Gregorevic et al. 2006; Wang et al. 2005). In normal neonate dogs, injection of AAV-9 resulted in expression of a reporter gene, in a dose-dependent manner, in widespread skeletal muscle but not in the heart (Yue et al. 2008). In another study, AAV-6 was the most efficient vector serotype in cardiac gene transfer following percutaneous transendo-cardial delivery to normal adult dogs (Bish et al. 2008). In both studies, no cellular immune responses were observed. More studies are required to determine whether these results could address safety scale-up issues in DMD dogs using a relevant therapeutic transgene, and whether the potential risk of immune responses to the vector and/or ...
There is an ongoing, intensive search for novel therapies to improve the prognosis of patients with the most common and aggressive form of primary brain cancer, glioblastoma multiforme (GBM). Gene transfer is one such approach. Early gene-transfer studies with replication incompetent vectors showed this approach to be generally safe, but ineffective due to limited transduction of the tumor. More recently gene transfer has been attempted with oncolytic, replicating viruses. However these viruses are rapidly cleared by the immune system. To overcome these shortcomings of previous gene transfer protocols, Toca 511 has been developed utilizing a Retroviral Replicating Vector (RRV). This platform has the following potential advantages: 1) The vector only infects dividing cells, 2) The virus stably integrates into the genome of the tumor cells allowing for the potential for long-term control of the tumor, 3) The virus is not intrinsically oncolytic and is not cleared from the tumor by the immune ...
ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.. About ASM , Contact Us , Press Room. ASM is a member of. ...
Cardiac gene transfer represents a unique strategy to alter the expression of specific genes in the heart that may result in alterations in contractile function. This approach allows us to investigate the role of these pathways on cardiac disease pathogenesis and progression. In rodents, cardiac gene transfer has been achieved predominantly by direct injection into the myocardium or perfusion of an isolated coronary segment. Either approach results in focal overexpression of the transgene and is therefore unlikely to effectively modulate global cardiac function. Here, we provide a gene transfer method that results in global transduction of the heart. We used this technique to demonstrate in vivo that beneficial effects of SUMO1 in the setting of heart failure are dependent on SERCA2a.. ...
Health, ...The challenge of treating patients with genetic disorders in which a s...A group of untreatable muscle disorders known as dysferlinopathies are...Gene therapy using adeno-associated virus (AAV) to deliver genes to ce... We have had success in the clinic using AAV gene therapy with limb gi...,New,gene,transfer,strategy,shows,promise,for,limb,girdle,and,other,muscular,dystrophies,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Progress in gene transfer technology has established the scientific basis for molecular approaches to human diseases. In the kidney, the first trial of gene transfer was reported in 1991, and several...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Human gene therapy is another medical application that is gaining wide acclaim. In essence, it is the transfer of genetic information into patient tissues and organs. As a result, diseased defective copies of genes can be eliminated or their normal functions rescued. Moreover, the procedure can provide new functions to cells. For Example, to combat cancer and other diseases, the insertion of a gene that causes the production of immune system mediator proteins can be introduced. By this therapy, countless genetic disease could have potential cures further down the road. There are two paths to Gene therapy. The first path is direct transfer of genes into the patient. The second path is the use of living cells as vehicles to transport the genes of interest. These two paths both have certain advantages and disadvantages. Direct gene transfer is the most simplistic way of administering the gene of choice. There are two methods to direct gene transfer. The first method is the process in which genes ...
RNAi technology has another key advantage. Scientists need very small doses of siRNA to carry out their experiments; only a small number of the molecules need to enter a cell to exert their silencing effect. On the other hand, says Hagstrom, the real bottleneck is that you have to get siRNA into cells. Thats not particularly difficult for cells in vitro. But transfecting siRNA into cells in vivo presents a more serious challenge. Mirus, a company that specializes in gene transfer technologies, has developed TransIT-TKO to carry out the task in vitro. Our formulation is a combination of a polymer and an amphipathic polyamine, says Hagstrom. were very far along with the technology for in vitro transfection. For in vivo transfection we have had one of the first publications showing highly effective nonviral gene delivery to a mouse liver. In this procedure the nucleic acid is delivered into the bloodstream in a manner that facilitates its delivery to cells outside the blood vessel wall. The ...
Knowledge about the uptake mechanism and subsequent intracellular routing of non-viral gene delivery systems is important for the development of more efficient carriers. In this study we compared two
e. Transformation frequency (% of randomly recovered colonies). In the second procedure, protoplasts were plated immediately after PEG treatment in a 2ml slab of 0.6% low melting agarose (Paszty and Lurquin, BioTechniques 5:716, 1987) in growth medium with 6% mannitol and 25% CM which was then overlaid with less than 0.5ml liquid growth medium also containing 6% mannitol and 25% CM. After 2 weeks, the slabs were sectioned and placed as above on solid nonselective growth medium for additional proliferation of cells for 7d before selection. Rapid growth of discrete microclusters was observed during this second round of growth on nonselective agar. These microclusters are called colonies since the original aggregates of PEG- treated protoplasts were maintained as unique entities embedded in agarose. This plating method does not allow further division into sib aggregates during growth on nonselective medium. Further, it is also possible that each original cluster of PEG-treated cells may have ...
Principal Investigator:UMEHARA Takashi, Project Period (FY):1999 - 2000, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Orthopaedic surgery
Sigma-Aldrich offers abstracts and full-text articles by [Ling Li, Fangfang Zhao, Baojing Zhao, Jin Zhang, Chao Li, Renzhong Qiao].
We have developed a proprietary gene therapy platform, the Skin TARgeted Delivery platform, or STAR-D platform, that consists of a patent pending engineered viral vector and skin-optimized gene transfer technology, to develop off-the-shelf treatments for dermatological diseases for which we believe there are no known effective treatments.. Our modified HSV-1 is a replication-deficient, non-integrating viral vector that can efficiently penetrate a broad range of skin cells. Its high payload capacity to accommodate large or multiple genes and low immunogenicity makes it a suitable choice for direct and repeat delivery to the skin.. ...
In vivo gene delivery with non-viral carriers. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Animal homologs of human genetic diseases are used as test systems for gene transfer by viral vectors. The approaches for transferring genes to the brain currently being investigated are ex vivo gene transfer using retrovirus and lentivius vector-modified neural stem cells transplanted to the brain and direct injection of herpesvirus, adeno-associated virus, and lentivirus vectors. The studies involve comparisons of promoters, properties of transduction for different cell types and various subregions of the brain. New methods to follow cell fate and gene expression in the live animal are being explored using MRI and PET techniques. Studies are also being directed towards better understanding of the mechanism of disease in the brain ...
Animal homologs of human genetic diseases are used as test systems for gene transfer by viral vectors. The approaches for transferring genes to the brain currently being investigated are ex vivo gene transfer using retrovirus and lentivius vector-modified neural stem cells transplanted to the brain and direct injection of herpesvirus, adeno-associated virus, and lentivirus vectors. The studies involve comparisons of promoters, properties of transduction for different cell types and various subregions of the brain. New methods to follow cell fate and gene expression in the live animal are being explored using MRI and PET techniques. Studies are also being directed towards better understanding of the mechanism of disease in the brain ...
PDF Gratis herunterladen Gene Therapy for HIV and Chronic Infections (Deutschland) - Ben Berkhout. This book centerson gene therapy and gene transfer approaches to prevent or treat chronic virus...
A command protocol for a single wire bus for transmitting and receiving commands and data. The command protocol includes a serial command word which facilitates communication between a host circuit an
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Internet-Draft HTTP/1.1, Part 2 July 2011 PUT successfully creates one, then the origin server MUST inform the user agent by sending a 201 (Created) response. If the target resource does have a current representation and that representation is successfully modified in accordance with the state of the enclosed representation, then either a 200 (OK) or 204 (No Content) response SHOULD be sent to indicate successful completion of the request. Unrecognized header fields SHOULD be ignored (i.e., not saved as part of the resource state). An origin server SHOULD verify that the PUT representation is consistent with any constraints which the server has for the target resource that cannot or will not be changed by the PUT. This is particularly important when the origin server uses internal configuration information related to the URI in order to set the values for representation metadata on GET responses. When a PUT representation is inconsistent with the target resource, the origin server SHOULD either ...
Since the isolation of the GFP from the bioluminescent jellyfish in 1992 and the subsequent development of related molecules, genetically encoded sensors that enable fluorescence imaging of excitable cell activity have been constructed by fusing fluorescent proteins to functional proteins that are involved in physiological signaling. Because these sensors can be introduced by gene transfer techniques, they may extract neuronal signals from an intact brain more efficiently than conventional organic dyes. Also, their expression is driven in a certain population of neurons by the use of a specific promoter; this has made visualization of the connectivity between two or more different (sub)populations of neurons all the more exciting ...
Since the isolation of the GFP from the bioluminescent jellyfish in 1992 and the subsequent development of related molecules, genetically encoded sensors that enable fluorescence imaging of excitable cell activity have been constructed by fusing fluorescent proteins to functional proteins that are involved in physiological signaling. Because these sensors can be introduced by gene transfer techniques, they may extract neuronal signals from an intact brain more efficiently than conventional organic dyes. Also, their expression is driven in a certain population of neurons by the use of a specific promoter; this has made visualization of the connectivity between two or more different (sub)populations of neurons all the more exciting ...
TY - JOUR. T1 - Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ. AU - Wolff, Gerhard. AU - Worgall, Stefan. AU - Van Rooijen, Nico. AU - Song, Wen Ru. AU - Harvey, Ben Gary. AU - Crystal, Ronald G.. PY - 1997/1/1. Y1 - 1997/1/1. N2 - Based on the hypothesis that tissue macrophages present an obstacle for adenovirus (Ad) vector-mediated gene transfer to internal organs, this study evaluated the consequences of transient depletion of Kupffer cells on subsequent transfer of the Ad vector genome and Ad vector-directed gene expression in the livers of experimental animals. Depletion of Kupffer cells in mice by intravenous administration of multilamellar liposomes containing dichloromethylene-bisphosphonate permitted subsequent intravenous administration of an Ad vector to provide a higher input of recombinant adenoviral DNA to the liver, an absolute increase in transgene expression, and a delayed clearance of the ...
Title: Virus-based Gene Transfer Approaches and Adipose Tissue Biology. VOLUME: 8 ISSUE: 2. Author(s):Louis Casteilla, Beatrice Cousin, Valerie Planat-Benard, Patrick Laharrague, Mamen Carmona and Luc Penicaud. Affiliation:UMR 5241 CNRS UPS IFR31, IFR109 Institut Louis Bugnard BP 84225, 31432 Toulouse Cedex 4,France.. Keywords:Adipose tissue, preadipocytes, adipose derived cells, virus, promoter, shRNA, cell therapy. Abstract: The status of adipose tissue changes rapidly. From a simple filler tissue, it successively acquires the status of metabolic active tissue, endocrine tissue, plastic tissue, and finally that of a large reservoir of cells suitable for cell therapy and regenerative medicine. All throughout this story, our knowledge has been largely dependent on genetic tools and gene transfer. Now, the time has come where gene transfer in adipose derived cells can be envisioned, not only for understanding the role or importance of one gene, but also to engineer adipose derived cells for the ...
Efficient gene transfer by vectors that can be easily delivered to target organs is desirable in clinical gene therapy. We tested the hypothesis that intracoronary infusion of the nitric oxide donor nitroprusside would increase the efficiency of adenovirus vector-mediated gene transfer to the heart. Intracoronary delivery of an adenovirus encoding murine adenylyl cyclase type VI (Ad.AC(VI)) was performed in adult pigs with and without simultaneous intracoronary infusion of nitroprusside. Animals were killed 12-14 days after Ad.AC(VI) delivery and myocardial adenylyl cyclase activity was measured. Addition of nitroprusside during intracoronary infusion of Ad.AC(VI) was associated with a 4-fold increase in cAMP-generating capacity in the left ventricle. Transgene expression was confirmed by immunoblotting. Intracoronary nitroprusside produced mild dose-dependent changes in blood pressure and heart rate during infusion. Intracoronary nitroprusside infusion is a safe and effective means to increase ...
Viral vectors are superior tools for gene therapy and as a genetic vaccine platform because viruses have evolved to efficiently infect and transfer their genomes to cells. Several impressive successes in viral vector-based gene therapies have been reported in humans, including restoration of vision in patients with Lebers congenital amaurosis by retinal gene transfer and cures for severe immune deficiencies by gene transfer to hematopoietic stem cells. However, the mammalian immune system has evolved in parallel to fend off invading pathogens such as viruses. Innate and antigen-specific adaptive immune responses against viral vectors and therapeutic transgene products pose serious hurdles for successful gene therapy. Pre-existing immunity in humans, resulting from prior exposure to the parent virus that forms the basis for the gene transfer vehicle may be derived from, often prevents efficient gene transfer. This problem also reduces our ability to use certain vectors for genetic vaccination or in anti
The successful delivery of therapeutic genes to the designated target cells and their availability at the intracellular site of action are crucial requirements for successful gene therapy. Nonviral gene delivery is currently a subject of increasing attention because of its relative safety and simplicity of use; however, its use is still far from being ideal because of its comparatively low efficiency. Most of the currently available nonviral gene vectors rely on two main components, cationic lipids and cationic polymers, and a variety of functional devices can be added to further optimize the systems. The design of these functional devices depends mainly on our understanding of the mechanisms involved in the cellular uptake and intracellular disposition of the therapeutic genes as well as their carriers. Macromolecules are internalized into cells by a variety of mechanisms, and their intracellular fate is usually linked to the entry mechanism. Therefore, the successful design of a nonviral gene ...
The potential of gene therapy is currently limited by the lack of delivery systems able to efficiently carry therapeutic DNA to their site of action. Non-viral vectors are receiving increasing attention as gene delivery vehicles due to the limitations associated with viral vectors in terms of safety and immunogenicity. Unfortunately, their use is hampered by their lower transfection efficacy compared with viral systems. The present study investigates the possibility of improving transfection by grafting amino acids onto the surface of a non-viral gene delivery system. We chose to use the amino acids arginine, lysine, and leucine because they have been reported to enhance transportation into cells (1-3). As a model delivery system, we chose to use the polymer polyethylenimine (PEI), because it has been widely used for non-viral transfection in vitro and in vivo and combines strong DNA compaction capacity with an intrinsic endosomolytic activity known as the proton sponge effect (4-5). It is ...
The use of biomaterials for gene delivery can potentially avoid many of the safety concerns with viral gene delivery. However, the efficacy of polymeric gene delivery methods is low, particularly in vivo. One significant concern is that the interior and exterior composition of polymeric gene delivery nanoparticles are often coupled, with a single polymer backbone governing all functions from biophysical properties of the polymer/DNA particle to DNA condensation and release. In this work we develop electrostatically adsorbed poly(glutamic acid)-based peptide coatings to alter the exterior composition of a core gene delivery particle and thereby affect tissue-specificity of gene delivery function in vivo. We find that with all coating formulations tested, the coatings reduce potential toxicity associated with uncoated cationic gene delivery nanoparticles following systemic injection. Particles coated with a low 2.5:1 peptide:DNA weight ratio (w/w) form large 2 μ sized particles in the presence of ...
TY - JOUR. T1 - Erythroviruses as gene transfer vehicles.. AU - Srivastava, A.. AU - Kurpad, C.. AU - Yoder, Mervin. PY - 2000. Y1 - 2000. UR - http://www.scopus.com/inward/record.url?scp=0033643705&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0033643705&partnerID=8YFLogxK. M3 - Article. C2 - 10941575. AN - SCOPUS:0033643705. VL - 4. SP - 133. EP - 148. JO - Contributions to Microbiology. JF - Contributions to Microbiology. SN - 1420-9519. ER - ...
TY - JOUR. T1 - pH-Sensitive cationic polymer gene delivery vehicle. T2 - N-Ac-poly(L-histidine)-graft-poly(L-lysine) comb shaped polymer. AU - Benns, J. M.. AU - Choi, J. S.. AU - Mahato, R. I.. AU - Park, J. S.. AU - Sung Wan Kim, Wan Kim. N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.. PY - 2000. Y1 - 2000. N2 - Advancing biotechnology spurs the development of new pharmaceutically engineered gene delivery vehicles. Poly(L-histidine) {PLH} has been shown to induce membrane fusion at endosomal pH values, whereas PLL has a well documented efficacy in polyplex formation. Therefore, N-Ac-poly(L-histidine)-graft-poly(L-lysine) {PLH-g-PLL} was synthesized by grafting poly(L-histidine) to poly(L-lysine) {PLL}. PLH-g-PLL formed polyplex particles by electrostatic interactions with plasmid DNA {pDNA}. The mean particle size of the polyplexes was in the range of 117 ± 6 nm to 306 ± 77 nm. PLH-g-PLL gene carrier demonstrated higher transfection efficacy in 293T cells than PLL at all ...
Over the last several years, various gene delivery systems have been developed for gene therapy applications. Although viral vector-based gene therapy has led
TY - JOUR. T1 - Reversal of potassium channel deficiency in cells from failing hearts by adenoviral gene transfer. T2 - A prototype for gene therapy for disorders of cardiac excitability and contractility. AU - Nuss, H. B.. AU - Johns, D. C.. AU - Kääb, S.. AU - Tomaselli, G. F.. AU - Kass, David A. AU - Lawrence, J. H.. AU - Marban, E.. PY - 1996. Y1 - 1996. N2 - Heart failure is a common, often lethal disorder in which conventional pharmacologic strategies have achieved limited success. Failing hearts exhibit a delay of electrical repolarization which predisposes to fatal arrhythmias. To explore the feasibility of gene therapy for this condition, we isolated myocytes from normal and failing dog hearts and quantified electrophysiologic and contractile parameters in primary culture. Action potentials were prolonged in failing cells as a result of diminished potassium currents. Exposure to AdShK, an adenovirus that overexpresses potassium channels, reversed the action potential prolongation of ...
The Hypertext Transfer Protocol (HTTP) is an application-level protocol with the lightness and speed necessary for distributed, collaborative, hypermedia information systems. HTTP has been in use by the World-Wide Web global information initiative since 1990. This specification reflects common usage of the protocol referred to as HTTP/1.0. This specification describes the features that seem to be consistently implemented in most HTTP/1.0 clients and servers. The specification is split into two sections. Those features of HTTP for which implementations are usually consistent are described in the main body of this document. Those features which have few or inconsistent implementations are listed in Appendix D. Practical information systems require more functionality than simple retrieval, including search, front-end update, and annotation. HTTP allows an open-ended set of methods to be used to indicate the purpose of a request. It builds on the discipline of reference provided by the Uniform ...
The majority of breast cancers are oestrogen dependent and although current treatment strategies have improved, approximately 50% of the patients will develop metastasis. New treatments that result in long-term systemic immunity are therefore being developed. We have previously shown that adenoviral gene transfer of B7-I/IL-2 to murine breast cancer induces a high rate of complete turnout regression and systemic immunity. Since oestrogens not only affect breast cancer but also have been shown to modulate immune function and secretion of immune-regulatory cytokines, we explored whether administration of oestradiol altered the immune response induced by an adenoviral vector expressing B7-I/IL-2. An oestrogen-dependent murine breast cancer tumour was used in ovariectomised mice, supplemented either oestradiol or placebo. We report the somewhat unexpected finding that intratumoral injection of adenovirus expressing B7-I/IL-2 induces complete turnout regression in 76% of oestradiol-supplemented mice, ...
Inherited retinal degenerative diseases are a clinically promising focus of adeno-associated virus (AAV)-mediated gene therapy. These diseases arise from pathogenic mutations in mRNA transcripts expressed in the eyes photoreceptor cells or retinal pigment epithelium (RPE), leading to cell death and structural deterioration. Because current gene delivery methods require an injurious subretinal injection to reach the photoreceptors or RPE and transduce just a fraction of the retina, they are suitable only for the treatment of rare degenerative diseases in which retinal structures remain intact. To address the need for broadly applicable gene delivery approaches, we implemented in vivo-directed evolution to engineer AAV variants that deliver the gene cargo to the outer retina after injection into the eyes easily accessible vitreous humor. This approach has general implications for situations in which dense tissue penetration poses a barrier for gene delivery. A resulting AAV variant mediated ...
This book is great to learn, produce, and apply several different types of viral and non-viral vectors for gene transfer. It provides all necessary information from beginning to the end for successful genaration of vectors. Troubleshooting guidelines is also very helpful ...
Gene therapy is recognized as a medical approach for the treatment of diseases that are difficult to cure, such as tumors. Several viral and non-viral carriers for gene transfer have been developed for either in vivo or ex vivo/in vitro use [1-4]. The most important viral carriers, characterized in laboratory studies and clinical trials, have been inactivated retroviruses, adenoviruses, adeno-associated viruses, and herpes viruses. These viruses show relatively high transfection efficiency, but have some clinical safety problems. On the other hand, non-viral methods of gene delivery, including cationic lipofection, calcium phosphate precipitation, gene guns, and injection of naked DNA, generally show low transfection efficiency but are not pathogenic [1-4]. However, there is another serious issue regarding the present gene delivery methodologies. Almost all the gene delivery methods currently developed have insufficient ability to specifically recognize and target diseased cells and to ...
A novel system for detection of gene transfer between A. tumefaciens and mammalian cells was established. Using this system, Agrobacterium was found to be able to deliver DNA located on its plasmid and chromosome into human cells. Agrobacterium is actively involved in this process; such a kind of DNA transfer might occur widely between bacteria and mammalian cells. The frequency of such a gene transfer was approximately 10-4-10-5 transformants per recipient. Our data showed that this DNA transfer is dependent upon Agrobacterium and independent of its virulence genes. The polymerization of actin played an important role in Agrobacterium internalization and subsequent gene transfer into mammalian cells. In addition, an Agrobacterium sensor protein ChvG was found to be important for the gene transfer into both plant and mammalian cells. The data suggested that ChvG was involved in the regulation of acid-inducible genes and might function as a global sensor protein that can directly or indirectly ...
This gene therapy study is being conducted to evaluate intramuscular gene transfer using VEGF (Vascular Endothelial Growth Factor) in patients with diabetic neuropathy in the legs. This condition causes a decrease in feeling and sensation due to diabetes. VEGF is DNA, or genetic material that is injected into the muscles of the leg. Once in the leg, it has been shown to cause new blood vessels to grow under a variety of conditions ...
Ex vivo gene delivery of GDNF using primary astrocytes transduced with a lentiviral vector provides neuroprotection in a rat model of Parkinsons disease.: Astr
Atsushi Miyanohara, Ph.D., is an experienced molecular biologist who has supervised the Vector Core Laboratory of the UCSD Program in Human Gene Therapy since its inception in 1995. He has longstanding extensive experience dealing with the design, preparation and properties of retrovirus, lentivirus, HSV1, adenovirus and AAV vectors. Dr. Miyanohara is one of the principals in the development of the VSV-G pseudotyping and VSV-G virosome technologies.. Dr. Miyanoharas major research focus has been development of safe and efficient gene transfer vectors not only for gene therapy applications, but also for a variety of basic research including stem cells, cell reprogramming and transgenesis.. As the Director of the Vector Core Laboratory, Dr. Miyanohara has extensive mutual research collaborations and has made important conceptual and technical advances in basic and clinical gene therapy, particularly in neurological and cardiovascular diseases.. Dr. Miyanohara received his Ph.D. in Medical ...
Since its discovery, evidence that siRNA was able to act as an RNA interference effector, led to its acceptation as a novel medicine. The siRNA approach is very effective, due to its catalytic mechanism, but still the limitations of its cellular delivery should be addressed. One promising form of non-viral gene delivery system is liposomes. The variable and versatile nature of the lipids keeps the possibility to upgrade the liposomal structure, which makes them suitable for encapsulation and delivery of drugs. However, to avoid the limitation of fast release for the hydrophilic drug, we previously designed viscous core liposomes. We aimed in this work to evaluate if these viscous core liposomes (NvcLs) could be of interest for siRNA encapsulation. Then, we sought to add a limited amount of positive charges to provide cell interaction and transfection. Cationic lipid dimyristoylaminopropylaminopropyl or the polymer poly(ethylenimine) were incorporated in NvcL to produce positively charged viscous ...
Human mesenchymal stem cells (hMSCs) are under study for cell and gene therapeutics because of their immunomodulatory and regenerative properties. Safe and efficient gene delivery could increase hMSC clinical potential by enabling expression of transgenes for control over factor production, behavior, and differentiation. Viral delivery is efficient but suffers from safety issues, while nonviral methods are safe but highly inefficient, especially in hMSCs. We previously demonstrated that priming cells with glucocorticoids (Gcs) before delivery of DNA complexes significantly increases hMSC transfection, which correlates with a rescue of transfection-induced metabolic and protein synthesis decline, and apoptosis. In this work, we show that transgene expression enhancement is mediated by transcriptional activation of endogenous hMSC genes by the cytosolic glucocorticoid receptor (cGR) and that transfection enhancement can be potentiated with a GR transcription-activation synergist. We demonstrate that the
This quote is deeply troubling. Genome sequencing did not reveal a new mechanism of evolution. And it is thus also inaccurate to say it would not have been discovered any other way. Lateral gene transfer was studies for many many many years before the first genome sequence was determined. Certainly, comparative genome analysis helped reveal the extent of gene transfer but it is seriously inaccurate to say it revealed a new mechanism of evolution. Here for example is a link to a google search for the specific phrase lateral gene transfer in papers published prior to 1995. And here is one for the phrase horizontal gene transfer ...
Robert Morris Sapolsky (born 1957) is an American neuroendocrinologist and author. He is currently a professor of biology, and professor of neurology and neurological sciences and, by courtesy, neurosurgery, at Stanford University. In addition, he is a research associate at the National Museums of Kenya.. As a neuroendocrinologist, he has focused his research on issues of stress and neuronal degeneration, as well as on the possibilities of gene therapy strategies for protecting susceptible neurons from disease. Currently, he is working on gene transfer techniques to strengthen neurons against the disabling effects of glucocorticoids. Each year Sapolsky spends time in Kenya studying a population of wild baboons in order to identify the sources of stress in their environment, and the relationship between personality and patterns of stress-related disease in these animals. More specifically, Sapolsky studies the cortisol levels between the alpha male and female and the subordinates to determine ...
The newly designed MT 3000-2i material transfer vehicle from Vögele offers exceptional performance in material transfer vehicles for asphalt paving. This machine is designed with the contractor in mind for unmatched productivity, exceptionally low operating costs and ultimate maneuverability. (ID::26188)
A number of neurodegenerative disorders, such as Parkinsons or Alzheimer Disease, may potentially be treated by gene therapy, i.e. the delivery of therapeutic genes to neurons. Currently, the most common carrier molecules to deliver the therapeutic gene to the patients target cells are viruses that have been genetically altered to carry normal human DNA. Overall gene delivery efficiency is typically low for nonviral vectors. New research undertaken at The Johns Hopkins University offers a systematic approach to understanding the gene delivery process in neurons and explores the intracellular barriers to nonviral gene delivery and possible ways to improve their effectiveness.
Th2 cytokines are associated with the airway inflammation and hyperreactivity in allergic asthma and are potential targets for developing novel therapies. The efficacy of a mucosal cytokine-gene transfer approach was examined in a mouse model for allergic asthma. We showed first that mucosal IFN-gamma gene transfer results in a significant expression of IFN-gamma in the pulmonary epithelium. Significantly, this approach inhibits both Ag- and Th2-cell-induced pulmonary eosinophilia and airway hyperreactivity. These findings suggest that mucosal IFN-gamma gene transfer is effective in modulating pulmonary allergic responses and provides a basis for developing a novel therapeutic approach. ...
The immune system confers protection against a variety of pathogens and contributes to the surveillance and destruction of neoplastic cells. Several cell types participate in the recognition and lysis of tumors, and appropriate immune stimulation provides therapeutic effects in malignancy. Foreign major histocompatibility complex (MHC) proteins also serve as a potent stimulus to the immune system. In this report, a foreign MHC gene was introduced directly into malignant tumors in vivo in an effort to stimulate tumor rejection. In contrast to previous attempts to induce tumor immunity by cell-mediated gene transfer, the recombinant gene was introduced directly into tumors in vivo. Expression of the murine class I H-2Ks gene within the CT26 mouse colon adenocarcinoma (H-2Kd) or the MCA 106 fibrosarcoma (H-2Kb) induced a cytotoxic T-cell response to H-2Ks and, more importantly, to other antigens present on unmodified tumor cells. This immune response attenuated tumor growth and caused complete ...
We have developed an endothelial specific gene delivery in vivo system (catalog number: EG6881) by combination of liposome mediated gene delivery and endothelial specific expression promoter vector to ensure target genes transferred in endothelial cells only for studying target gene functions in endothelial cell proliferation, differentiation, and death in vivo. The non-viral in vivo gene delivery system will be able to mediate endothelial-related target genes expressed only in endothelial cells. ...
Targeted gene delivery using non-viral vectors is a highly touted scheme to reduce the potential for toxic or immunological side effects by reducing dose. In previous reports, TAT polyplexes with DNA have shown relatively poor gene delivery. The transfection efficiency has been enhanced by condensing TAT/DNA complexes to a small particle size using calcium. To explore the targetability of these condensed TAT complexes, LABL peptide targeting intercellular cell-adhesion molecule-1 (ICAM-1) was conjugated to TAT peptide using a polyethylene glycol (PEG) spacer. PEGylation reduced the transfection efficiency of TAT, but TAT complexes targeting ICAM-1 expressing cells regained much of the lost transfection efficiency. Targeted block peptides properly formulated with calcium offer promise for gene delivery to ICAM-1 expressing cells at sites of injury or inflammation. ...
Definition of environment in regard to interaction with genotypes can be widen to include sexes and major genes. Interaction of sexes with genotypes has been found in meat-type chickens under extreme environmental conditions. In this case, sexes are regarded as environments. Interaction of sexes with environments has been also reported. It appears that overall efficiency of broiler production can be improved by placing males in more optimal environments, and females in less optimal ones. Interaction of major genes for disease resistance with genotypes, i.e., genetic background was found to affect their level of expression. This type of interaction is essential to the utilization of gene transfer technology; an appropriate method for its analysis is described ...
This protocol describes the method, materials, equipment and steps for bottom-up preparation of RNA and protein producing synthetic...
The CRC32c algorithm is a 32-bit cyclic redundancy check. It achieves a better hamming distance (for better error-detection performance) than many other 32-bit CRC functions. Other places it is used include iSCSI and SCTP. The 32-bit output is encoded in hexadecimal (using between 1 and 8 ASCII characters from 0-9, A-F, and a-f; leading 0s are allowed). For example, CRC32c=0a72a4df and crc32c=A72A4DF are both valid checksums for the 3-byte message dog ...
Indd 41 11/8/2013 12:05:31 PM B1574 Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology 42 Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology cause a reduction in expression if there are six to seven consecutive matches between the 5 end of siRNA guide strand and the off-target mRNA sequence. Some rules now included in web software for design of siRNA sequences attempt to ensure that this partial complementarity is avoided. The most critical non-sequence-specific effect of siRNAs is the induction of the immune response. 3 Non-target-related effects Unexpected effects of antisense oligonucleotides and more specifically oligophosphorothioates, unrelated to their RNA binding, have been described. These nonantisense effects can be both non-sequence-specific (resulting from the polyanionic nature of the oligonucleotides) or sequence specific. The non-antisense effects have been mainly described with unmodified oligonucleotides and with ...
Lipid Polycation DNA (LPD) is Targeted Genetics newest synthetic gene delivery system. Targeted Genetics, in collaboration with Dr L Huang at the University of
Health,Researchers at the Salk Institute for Biological Studies say that even...The NMDA receptor is activated by the neurotransmitter glutamate ... When we removed the NMDA receptor that is when cells make connec...Combining mouse genetics and gene transfer techniques Gage and a ...A few weeks later animals that received the virus showed fewer fl...,Fittest,Of,Newborn,Neurons,Survive,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Mice were anesthetized with isoflurane induction and endotracheally intubated. Animals were placed supine on a thermoregulated table (37°C) and ventilated with isoflurane (2%) vaporized in 100% O2 using a custom constant-flow ventilator (6.7 μL/g−1 tidal volume at 120 minutes−1). ECG and core temperature were monitored. The left external jugular vein was cannulated, and modest volume expansion was provided (100 to 150 μL of 12.5% human albumin) at 30 μL min−1.. Topical 2% lidocaine gel was applied to the chest, and the thorax was entered by 1 of 2 techniques. For the first method, virus was injected into the LV chamber. Mice were placed on their right sides with the left forepaw affixed to the surgical table. The point of maximal intensity was visualized (approximately 0.5 to 0.75 cm below the axilla), and an incision was made to the left side of the point of maximal intensity. The thorax was carefully entered, avoiding cardiac injury, and the thoracotomy was widened by blunt ...
HUNG, G, G MUELLER, J GALEALAURI, H GEORGESCU, L MCINTYRE, M TINDAL, P ROBBINS, and C EVANS. 1993. INTRAARTICULAR EXPRESSION OF IRAP BY GENE-TRANSFER - INHIBITION OF IL-1-INDUCED PATHOLOGY. ARTHRITIS AND RHEUMATISM 36 (9): S46-S46 ...
One realization that has come from comparing multiple bacterial genome sequences, including multiple isolates from the same species, is that gene transfer is an important force in bacterial genome evolution. In the laboratory gene transfer is essential for the study of bacteria and for learning more about all living organisms. Three processes in bacteria can broadly define the transfer of DNA: transformation, transduction, and conjugation. This chapter focuses on the many genetic tools available to manipulate the genetic content of Escherichia coli. A DNA molecule that does not have its own origin of replication must integrate into either the host chromosome or another autonomously replicating element such as an endogenous plasmid. In E. coli a modified derivative of the bacteriophage T4 offers some advantages for transduction in that it packages twice as much DNA as P1 and also is less sensitive to capsules found on many pathogenic strains of E. coli. Transformation of bacteria by use of either
A means for obtaining efficient introduction of exogenous genes into a patient, with long term expression of the gene, is disclosed. The gene, under control of an appropriate promoter for expression in a particular cell type, is encapsulated or dispersed with a biocompatible, preferably biodegradable polymeric matrix, where the gene is able to diffuse out of the matrix over an extended period of time, for example, a period of three to twelve months or longer. The matrix is preferably in the form of a microparticle such as a microsphere (where the gene is dispersed throughout a solid polymeric matrix) or microcapsule (gene is stored in the core of a polymeric shell), a film, an implant, or a coating on a device such as a stent. The size and composition of the polymeric device is selected to result in favorable release kinetics in tissue. The size is also selected according to the method of delivery which is to be used, typically injection or administration of a suspension by aerosol into the nasal and/or
A means for obtaining efficient introduction of exogenous genes into a patient, with long term expression of the gene, is disclosed. The gene, under control of an appropriate promoter for expression in a particular cell type, is encapsulated or dispersed with a biocompatible, preferably biodegradable polymeric matrix, where the gene is able to diffuse out of the matrix over an extended period of time, for example, a period of three to twelve months or longer. The matrix is preferably in the form of a microparticle such as a microsphere (where the gene is dispersed throughout a solid polymeric matrix) or microcapsule (gene is stored in the core of a polymeric shell), a film, an implant, or a coating on a device such as a stent. The size and composition of the polymeric device is selected to result in favorable release kinetics in tissue. The size is also selected according to the method of delivery which is to be used, typically injection or administration of a suspension by aerosol into the nasal and/or
A means for obtaining efficient introduction of exogenous genes into a patient, with long term expression of the gene, is disclosed. The gene, under control of an appropriate promoter for expression in a particular cell type, is encapsulated or dispersed with a biocompatible, preferably biodegradable polymeric matrix, where the gene is able to diffuse out of the matrix over an extended period of time, for example, a period of three to twelve months or longer. The matrix is preferably in the form of a microparticle such as a microsphere (where the gene is dispersed throughout a solid polymeric matrix) or microcapsule (gene is stored in the core of a polymeric shell), a film, an implant, or a coating on a device such as a stent. The size and composition of the polymeric device is selected to result in favorable release kinetics in tissue. The size is also selected according to the method of delivery which is to be used, typically injection or administration of a suspension by aerosol into the nasal and/or
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Researchers at the University of Toronto announced today that IXmaps, a visual, interactive database of Internet traffic routes, is now live. The tool, funded by the .CA Community Investment Program, helps Canadians understand ...
Sigma-Aldrich offers abstracts and full-text articles by [Kai Ewert, Ayesha Ahmad, Heather M Evans, Hans-Werner Schmidt, Cyrus R Safinya].
Adeno-associated viruses (AAVs) are commonly used for in vivo gene transfer. Nevertheless, AAVs that provide efficient transduction across specific organs or cell populations are needed. Here, we describe AAV-PHP.eB and AAV-PHP.S, capsids that efficiently transduce the central and peripheral nervous …
Virtually all cells in the organism secrete extracellular vesicles (EVs), a heterogeneous population of lipid bilayer membrane-enclosed vesicles that transport and deliver payloads of proteins and nucleic acids to recipient cells, thus playing central roles in cell-cell communications. Exosomes, nan …
Scientists led by University of Iowa researcher Paul McCray, Jr., MD, are using simple peptides to deliver gene-editing tools into notoriously hard-to-access lung and airway cells with the goal of creating new treatments for people with diseases like cystic fibrosis, COPD, and asthma.
Identification of Genes of VSH-1, a Prophage-Like Gene Transfer Agent of Brachyspira hyodysenteriae Eric G. Matson, M. Greg ... Gene Transfer between Related Bacteria by Electrotransformation: Mapping Salmonella typhi Genes in Salmonella typhimurium ... Gene Transfer and Genome Plasticity in Thermotoga maritima, a Model Hyperthermophilic Species Emmanuel F. Mongodin, Ioana R. ... Role of Agrobacterium virB Genes in Transfer of T Complexes and RSF1010 Karla Jean Fullner ...
The Impact of Gene Transfer Techniques in Eukaryotic CeU Biology brought together a number of speakers interested in various ... The 35th N:osbach Colloquium "The Impact of Gene Transfer Techniques in Eukaryotic CeU Biology" brought together a number of ... Genes Involved in Resistance Reactions in Higher Plants: Possible Candidates for Gene Transfer? ... An Alternative Gene Cloning Method for the Isolation of Human Genes by Expression in Mouse Cell Clones ...
Associations of Gene Transfer Techniques with chemical compounds. *Novel and effective gene transfer technique for study of ... Anatomical context of Gene Transfer Techniques. *We thus employed the adenovirus-mediated gene transfer technique, and we ... Psychiatry related information on Gene Transfer Techniques. *Furthermore, gene transfer techniques using recombinant ... using gene transfer technique [3].. *DNA-mediated gene transfer techniques have been used to study the effectiveness of a novel ...
Hum Gene Ther. 1995 Apr;6(4):445-55. Comparative Study; Research Support, Non-U.S. Govt; Research Support, U.S. Govt, P.H.S. ... the airway epithelium of CF patients has been targeted for studies of gene transfer. The biological efficacy of gene transfer ... In vivo nasal potential difference: techniques and protocols for assessing efficacy of gene transfer in cystic fibrosis.. ... and can yield estimates of the biological efficacy of gene transfer to achieve correction of the electrolyte transport defects ...
NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.(Statistical Data Included). ... ADAMO, JOSEPH A.; MICHAEL GEALT,. "NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.( ... ADAMO, JOSEPH A.; MICHAEL GEALT,. "NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.( ... ADAMO, JOSEPH A.; MICHAEL GEALT,. "NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.( ...
Gene transfer techniques. There are two primary approaches for gene transfer:. *In vivo gene therapy, where genetic material is ... In this article, we review gene transfer techniques and gene delivery systems that could potentially be used for treating ... Viral vectors for gene delivery. The epidermis was one of the first targets for in vivo experimental gene transfer, where ... Non-viral gene transfer is typically characterized by short-term gene expression and low transfection efficiency, which may be ...
Gene Transfer Technique. Introduction Gene transfer technique is used very widely both in basic research and applied biology. ... Gene transfer is one of the key factors in gene therapy, and it is one of the key purposes of the clone. Gene transfer can be ... The gene transfer results can be transient and stable transfection.. Gene therapy can be defined as the deliberate transfer of ... Gene transfer techniques for higher plants and animals are complex and costly. It is generally not done in laboratory scale ...
To test whether these genes have been acquired from bacteria through horizontal gene transfer, firstly, the distribution of ... Techniques *RNA and DNA isolation. *Genome walking and RACE *Bioinformatics analysis. *BAC library construction and screening ... Horizontal gene transfer. The driving force behind the evolution of plant parasitic nematodes?. ... us clues for the function of a gene.. In this way we are able to silence nematode genes that are putative parasitism related, ...
... it is often necessary to deliver genes to specific cells. In Gene Delivery t ... Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, ... Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the ... An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and ...
... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ...
Fingerprint Dive into the research topics of New therapeutic approaches based on gene transfer techniques. Together they form ...
Advancing Techniques Reveal the Brains Impressive Diversity. By Sara B. Linker, Tracy A. Bedrosian, and Fred H. Gage , ... Combined transcription and genome data from multiple tissues in hundreds of human donors reveal links between genotype and gene ... Small trials using younger donors and elderly recipients hint that mesenchymal stem cell transfers might reduce frailty. ... tags: horizontal gene transfer x genetics & genomics x The Scientist. » horizontal gene transfer and genetics & genomics ...
In this paper I review some of the techniques that can be used for gene transfer, and speculate on how they might be applied to ... First, the gene of interest is purified, usually by cloning in bacteria, and its structure is determined. Second, biochemical ... techniques are used to introduce mutations into the regions of interest. Finally, the normal and mutant genes are introduced ... In this paper I review some of the techniques that can be used for gene transfer, and speculate on how they might be applied to ...
Bacterial survival in the host and in the environment is promoted by horizontal gene transfer of additional metal resistance ... genes at University of Leicester, listed on FindAPhD.com ... Techniques that will be undertaken during the project. ... 2018 A horizontally gene transferred copper resistance locus confers hyper-resistance to antibacterial copper toxicity and ... Bacterial survival in the host and in the environment is promoted by horizontal gene transfer of additional metal resistance ...
... (or HGT) is the process of an organism acquiring another organisms DNA, usually through absorbing the ... Raman Spectroscopy as a Universal Analytical Technique for Bodily Fluids. Professor Igor K. Lednev ... Liverpool University recently conducted some research on Horizontal Gene Transfer. Sources. *Horizontal Gene Transfer and ... Horizontal Gene Transfer. News-Medical. 21 July 2019. ,https://www.news-medical.net/life-sciences/Horizontal-Gene-Transfer. ...
Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be ... the most commonly used methods employ replication-defective viral vectors for gene transfer, while physical gene transfer ... Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. ... Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be ...
Gene Transfer Techniques. Additional relevant MeSH terms: Lymphoma. HIV Infections. Lymphoma, Non-Hodgkin. Neoplasms by ... MedlinePlus related topics: Genes and Gene Therapy HIV/AIDS Lymphoma Genetic and Rare Diseases Information Center resources: ... Gene Therapy in HIV-Positive Patients With Non-Hodgkins Lymphoma. The safety and scientific validity of this study is the ... A Phase IIA Study of Comparative Marking Using a Ribozyme Gene and a Neutral Gene. ...
Gene Transfer Techniques. Additional relevant MeSH terms: Thalassemia. beta-Thalassemia. Anemia, Hemolytic, Congenital. Anemia ... Gene transfer, achieved by transplantation of the patients own blood stem cells that have been genetically-modified with the ... The first step in developing gene transfer for treatment of thalassemia is to develop a safe and effective method to obtain ... Eventually, high numbers of genetically modified cells will need to be infused into the patient for clinical gene transfer to ...
Gene Transfer Techniques. Additional relevant MeSH terms:. Layout table for MeSH terms. ... Gene transfer involves obtaining blood stem cells from an individual, adding a normal globin gene to the stem cells, and ... Before gene transfer methods can be attempted in individuals with beta thalassemia major, a safe method of obtaining blood stem ... A long-term goal of study researchers is to develop a gene transfer process as a method of curing beta thalassemia major. ...
Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8 ... I would point out that some of these genes (and even plasmid) transfers are cases of Lateral Gene Transfer, and not necessarily ... The acquisition of these genes may have occurred through horizontal gene transfer. The analysis further showed that this deadly ... http://en.citizendium.org/wiki/Horizontal_gene_transfer_in_prokaryotes. 1928. Discovery of gene transfer in bacteria started in ...
Gene transfer into CD4+ T lymphocytes: green fluorescent protein-engineered, encephalitogenic T cells illuminate brain ... Gene Transfer Techniques* * Genetic Engineering / methods * Genetic Vectors * Green Fluorescent Proteins * Luminescent Proteins ... Gene transfer into CD4+ T lymphocytes: green fluorescent protein-engineered, encephalitogenic T cells illuminate brain ...
One form of the disease, LCA2, is caused by mutations in the retinal pigment epithelium-specific 65-kDa protein gene (RPE65). ... Safety and efficacy of gene transfer for Lebers congenital amaurosis N Engl J Med. 2008 May 22;358(21):2240-8. doi: 10.1056/ ... Although the follow-up was very short and normal vision was not achieved, this study provides the basis for further gene ... One form of the disease, LCA2, is caused by mutations in the retinal pigment epithelium-specific 65-kDa protein gene (RPE65). ...
Morbidity and mobile genes - Investigating the role of gene transfer in the emergence of pathogenic vibrio ... We combined quantitative PCR and multiplex PCR/reverse line blot techniques to track patterns in the occurrence of 31 AbR genes ... Pathogenic Vibrio species (identification of genes involved in disease, particularly those derived by lateral gene transfer - ... Lateral gene transfer (LGT) impacts on the evolution of prokaryotes in both the short and long-term. The short-term impacts of ...
Recombinant DNA techniques.Strain TOP10 was used for standard recombinant DNA techniques. Plasmid DNA was prepared by using a ... sphaeroides flagellar genes, while the genes in the fla1 cluster were likely acquired as the result of a horizontal transfer ... Successful lateral transfer requires codon usage compatibility between foreign genes and recipient genomes. Mol. Biol. Evol. 21 ... Bacterial type III secretion systems are ancient and evolved by multiple horizontal-transfer events. Gene 312 : 151-163. ...
Recombinant DNA techniques *Gene transfer *Monoclonal antibodies *Gene cloning (Click here for description of the process of ...
... transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression ... PART II: THE TECHNIQUES FOR GENE TRANSFER. Gene Transfer into Mammal Embryos ... Gene Transfer into Fish and Lower Vertebrates. Gene Transfer into Invertebrates. The Use of ES Cells for Gene Transfer and Gene ... It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant ...
Using a gene transfer technique to study the role of endothelial nitric oxide synthase in neovascularization and myocardial ... Gene Transfer Techniques. Neovascularization, Pathologic. Adenoviridae. Coronary Artery Disease Collection MUSC Theses and ... The effects of eNOS gene delivery were also evaluated in a rat model of myocardial infarction. Six weeks after gene transfer, ... Using a gene transfer technique to study the role of endothelial nitric oxide synthase in neovascularization and myocardial ...
Dive into the research topics of A novel in vivo gene transfer technique and in vitro cell based assays for the study of bone ... A novel in vivo gene transfer technique and in vitro cell based assays for the study of bone loss in musculoskeletal disorders ...
New technique pinpoints milestones in the evolution of bacteria. Bacteria have evolved all manner of adaptations to live in ... Studying the hotbed of horizontal gene transfers. For over 200,000 years, humans and their gut microbiomes have coevolved into ... Microbes in the human body swap genes, even across tissue boundaries: study. Bacteria in the human body are sharing genes with ... Yeasts reach across tree of life to domesticate suite of bacterial genes. An insects gut might seem an inhospitable place to ...
New technique pinpoints milestones in the evolution of bacteria. Bacteria have evolved all manner of adaptations to live in ... Cross species transfer of genes has driven evolution. Far from just being the product of our parents, University of Adelaide ... New gene transfer rules could help prevent spread of antibiotic resistance. Unlike other organisms, bacteria can take up ... Studying the hotbed of horizontal gene transfers. For over 200,000 years, humans and their gut microbiomes have coevolved into ...
  • Doctors for the first time have used a form of gene therapy to restore partial vision in a blind person, according to findings announced Monday. (futuretimeline.net)
  • at present [url+http://en.wikipedia.org/wiki/Gene_therapy]gene therapy[/url] as practised / as envisaged is much more viable and has fewer ethical and moral hurdles. (thenakedscientists.com)

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