Gene Transfer Techniques
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
Moving and Lifting Patients
Genetic Vectors
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Genetic Therapy
Transfection
Embryo Transfer
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
Fluorescence Resonance Energy Transfer
Bioluminescence Resonance Energy Transfer Techniques
Gene Transfer, Horizontal
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
Energy Transfer
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
Adenoviridae
Cells, Cultured
Pregnancy
Transduction, Genetic
Nuclear Transfer Techniques
Dependovirus
Retroviridae
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
beta-Galactosidase
Gene Expression
Plasmids
Transfer (Psychology)
Electroporation
A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Bacteriology
Sequence Analysis, DNA
Evolution, Molecular
Oligonucleotides, Antisense
Adenosine Deaminase
RNA, Antisense
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
Immunologic Deficiency Syndromes
DNA, Antisense
DNA that is complementary to the sense strand. (The sense strand has the same sequence as the mRNA transcript. The antisense strand is the template for mRNA synthesis.) Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes.
Polyethyleneimine
Public Opinion
Bone Marrow Transplantation
Developmental Biology
Transplantation, Homologous
Cytomegalovirus Infections
Allergy and Immunology
Graft vs Host Disease
Tropical Medicine
Publishing
Bibliometrics
Eukaryotic Cells
CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/7311)
Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors. (+info)Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (2/7311)
Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis. (+info)Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (3/7311)
Herpes simplex virus type 1 (HSV-1) has many attractive features that suggest its utility for gene transfer to neurons. However, viral cytotoxicity and transient transgene expression limit practical applications even in the absence of viral replication. Mutant viruses deleted for the immediate early (IE) gene, ICP4, an essential transcriptional transactivator, are toxic to many cell types in culture in which only the remaining IE genes are expressed. In order to test directly the toxicity of other IE gene products in neurons and develop a mutant background capable of longterm transgene expression, we generated mutants deleted for multiple IE genes in various combinations and tested their relative cytotoxicity in 9L rat gliosarcoma cells, Vero monkey kidney cells, and primary rat cortical and dorsal root neurons in culture. Viral mutants deleted simultaneously for the IE genes encoding ICP4, ICP22 and ICP27 showed substantially reduced cytotoxicity compared with viruses deleted for ICP4 alone or ICP4 in combination with either ICP22, ICP27 or ICP47. Infection of neurons in culture with these triple IE deletion mutants substantially enhanced cell survival and permitted transgene expression for over 21 days. Such mutants may prove useful for efficient gene transfer and extended transgene expression in neurons in vitro and in vivo. (+info)Treatment of lysosomal storage disease in MPS VII mice using a recombinant adeno-associated virus. (4/7311)
Mucopolysaccharidosis type VII (MPS VII) is a lysosomal storage disease caused by a genetic deficiency of beta-glucuronidase (GUS). We used a recombinant adeno-associated virus vector (AAV-GUS) to deliver GUS cDNA to MPS VII mice. The route of vector administration had a dramatic effect on the extent and distribution of GUS activity. Intramuscular injection of AAV-GUS resulted in high, localized production of GUS, while intravenous administration produced low GUS activity in several tissues. This latter treatment of MPS VII mice reduced glycosaminoglycan levels in the liver to normal and reduced storage granules dramatically. We show that a single administration of AAV-GUS can provide sustained expression of GUS in a variety of cell types and is sufficient to reverse the disease phenotype at least in the liver. (+info)Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (5/7311)
Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain. (+info)High level inhibition of HIV replication with combination RNA decoys expressed from an HIV-Tat inducible vector. (6/7311)
Intracellular immunization, an antiviral gene therapy approach based on the introduction of DNA into cells to stably express molecules for the inhibition of viral gene expression and replication, has been suggested for inhibition of HIV infection. Since the Tat and Rev proteins play a critical role in HIV regulation, RNA decoys and ribozymes of these sequences have potential as therapeutic molecular inhibitors. In the present study, we have generated several anti-HIV molecules; a tat-ribozyme, RRE, RWZ6 and TAR decoys and combinations of decoys, and tested them for inhibition of HIV-1 replication in vitro. We used T cell specific CD2 gene elements and regulatory the HIV inducible promoter to direct high level expression and a 3' UTR sequence for mRNA stabilization. We show that HIV replication was most strongly inhibited with the combination TAR + RRE decoy when compared with the single decoys or the tat-ribozyme. We also show that the Tat-inducible HIV promoter directs a higher level of steady-state transcription of decoys and inhibitors and that higher levels of expression directly relate to increased levels of inhibition of HIV infection. Furthermore, a stabilization of the 3' end of TAR + RRE inhibitor transcripts using a beta-globin 3' UTR sequence leads to an additional 15-fold increase in steady-state RNA levels. This cassette when used to express the best combination decoy inhibitor TAR + RRE, yields high level HIV inhibition for greater than 3 weeks. Taken together, both optimization for high level expression of molecular inhibitors and use of combinations of inhibitors suggest better therapeutic application in limiting the spread of HIV. (+info)Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (7/7311)
Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity. (+info)Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (8/7311)
We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located. (+info)
Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer. | BMC Biotechnology | Full Text
Non-viral gene delivery vectors were developed for efficient gene transfer to | FGFR signaling promotes the growth of triple...
Frontiers | Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases | Pharmacology
Secure File Transfer Protocol Jobs in Valsad, Gujarat (Sep 2021) - 6 Secure File Transfer Protocol Openings in Valsad, Gujarat ...
Development of novel therapeutic approach using gene delivery method for treatment of cardiovascular disease | Department of...
Gene transfer strategies: principles, state-of-the-art and the major barriers that need to be overcome 2 | HSTalks
Mouse monoclonal to STYK1 | Small molecule inhibitors of HCV replication
Download Gene Delivery To Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques 2004
Comparison between agrobacterium-mediated and direct gene transfer using the gene gun.
Viral gene transfer - Biocenter Finland
Direct Gene Transfer with IP-10 Mutant Ameliorates Mouse CVB3-Induced Myocarditis by Blunting Th1 Immune Responses - pdf...
Somatic Gene Therapy | Universitätsklinikum Ulm
Cationic lipid-based gene delivery systems: Pharmaceutical perspectives<...
Lecture 9 - PLB 113 Lecture 9 III Gene Transfer and Epigenetic C Development of Gene Transfer System D Transgene Expression E...
A single-use chromatographic purification platform for viral gene transfer vectors & viral vaccines :: MPG.PuRe
Polymer-Based Gene Delivery: A Current Review on the Uptake and Intracellular Trafficking of Polyplexes | Bentham Science
RFC 354 - File Transfer Protocol
Klinische Studie und experimentelle Untersuchungen zur nicht-viralen Gentherapie solider Tumoren
Adenovirus-Augmented, Receptor-Mediated Gene Delivery and Some Solutions to the Common Toxicity Problems | Springer for...
File Transfer Protocol, FTP - Redorbit
Systemic Delivery of Therapeutic Genes in DMD Models - Muscular Dystrophy
A Study of a Retroviral Replicating Vector Administered to Subjects With Recurrent Malignant Glioma - Full Text View -...
Gene Transfer Techniques | Journal of Bacteriology
Scientific Protocols -
Efficient Viral Gene Transfer to Rat Hearts In Vivo
New gene transfer strategy shows promise for limb girdle and other muscular d... ( The challenge of treating patients w...)
Strategic gene transfer into the kidney: Current status and prospects | SpringerLink
DI-fusion Increased fluid secretion after adenoviral-mediated transfer...
Structural Biochemistry/Nucleic Acid/DNA/Transgenic Animals - Wikibooks, open books for an open world
Drug Discovery and Biotechnology Trends: Functional Genomics: Bridging the Knowledge Gap
Cellular Uptake of Cationic Polymer-DNA Complexes Via Caveolae Plays a Pivotal Role in Gene Transfection in COS-7 Cells |...
Chemical methods for direct gene transfer to proto-plasts
KAKEN - Research Projects | Direct Gene Transfer Into Articular Cartilage By Gene Gun (KAKENHI-PROJECT-11671438)
Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation,...
STAR-D Gene Therapy Platform - Krystal Biotech, Inc
In vivo gene delivery with non-viral carriers - pdf descargar
UPENN Biomedical Graduate Studies | John H. Wolfe
John H. Wolfe | Faculty | About Us | Perelman School of Medicine | Perelman School of Medicine at the University of Pennsylvania
Kostenloser Download für Bücher Gene Therapy for HIV and Chronic Infections (Deutschland) PDF
US Patent # 5,809,518. Command/data transfer protocol for one-wire-bus architecture - Patents.com
Ionic Bonding and Electron Transfer | Protocol (Translated to Turkish)
draft-ietf-httpbis-p2-semantics-15 - Hypertext Transfer Protocol (HTTP/1.1): Semantics and Content
Neuroscience2014 | Per session
Neuroscience2014 | Per session
Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target...
Virus-based Gene Transfer Approaches and Adipose Tissue Biology | Bentham Science
Nitroprusside increases gene transfer associated with intracoronary delivery of adenovirus. | PubFacts.com
Approaches to Blocking the Immune Response to Gene Transfer with Viral Vectors | Frontiers Research Topic
Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery | Pharmacological Reviews
Enhanced transfection efficacy of polyethylenimine by surface modification with arginine, lysine, and leucine - Strathprints
Tissue-Specific Gene Delivery via Nanoparticle Coating
Erythroviruses as gene transfer vehicles.<...
pH-Sensitive cationic polymer gene delivery vehicle: N-Ac-poly(L-histidine)-graft-poly(L-lysine) comb shaped polymer<...
Progress in Cationic Lipid-Mediated Gene Transfection: A Se
Reversal of potassium channel deficiency in cells from failing hearts by adenoviral gene transfer: A prototype for gene therapy...
Hypertext Transfer Protocol -- HTTP/1.0
Oestradiol enhances tumour regression induced by B7-I/IL-2 adenoviral gene transfer in a murine model of breast cancer
In Vivo-Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer Retinal Gene Delivery from the Vitreous |...
LeDoux J. Gene Therapy Protocols: Volume 1: Production and In Vivo Applications of Gene Transfer Vectors (Methods in...
Regulation of Transgene Expression in Tumor Cells by Exploiting Endogenous Intracellular Signals | Nanoscale Research Letters |...
Molecular basis of Agrobacterium-mediated gene transfer into mammalian cells | [email protected]
VEGF Gene Transfer for Diabetic Neuropathy - Full Text View - ClinicalTrials.gov
Ex vivo gene delivery of GDNF using primary astrocytes transdu...
About the Lab - Miyanohara Lab - UC San Diego Department of Pediatrics
bims-novged 2021-05-02 papers
Glucocorticoid Priming of Nonviral Gene Delivery to hMSCs Increases Tr by Andrew Hamann, Tyler Kozisek et al.
The Tree of Life: Ugg - story about gene transfer/evolution based on NSF press release has a NASA-esque smell
Robert Sapolsky - Wisdom Reel
The MT 3000-2i from Vögele Offers Exceptional Performance in New-Design Material Transfer Vehicle :: Story ID: 26188 ::...
Enhancing gene delivery with nanoparticles could ultimately lead to a cure for Alzheimers
Mucosal IFN-gamma gene transfer inhibits pulmonary allergic responses in mice. | The Journal of Immunology
Immunotherapy of malignancy by in vivo gene transfer into tumors. | PNAS
Liposome-mediated target gene transfer into mouse endothelial cells in vivo
Calcium-crosslinked LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cells
Genotype by environment interactions in poultry. | WCGALP Archive
Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer | Protocol
Hypertext Transfer Protocol (HTTP) Digest Algorithm Values
Advanced Textbook on Gene Transer, Gene Therapy and Genetic by Daniel Scherman - Novoline Library
Lipid polycation DNA gene delivery system - Targeted Genetics/Elan - AdisInsight
Fittest Of Newborn Neurons Survive ( Researchers at the Salk Institute for B...)
Robust Adenoviral and Adeno-Associated Viral Gene Transfer to the In Vivo Murine Heart | Circulation
INTRAARTICULAR EXPRESSION OF IRAP BY GENE-TRANSFER - INHIBITION OF IL-1-INDUCED PATHOLOGY - ORA - Oxford University Research...
ASMscience | Gene Transfer in Gram-Ne
Patent US20010020011 - Polymeric gene delivery system - Google Patents
Patent US20010020011 - Polymeric gene delivery system - Google Patents
Patent US20010020011 - Polymeric gene delivery system - Google Patentsuche
DI-fusion Efficient gene transfer to dispersed human pancreatic islet...
Researchers determine where Canadian data transfers increase exposure to US state surveillance
Efficient synthesis and cell-transfection properties of a new multivalent cationic lipid for nonviral gene delivery. | Sigma...
Engineered AAVs for Efficient Noninvasive Gene Delivery to the Central and Peripheral Nervous Systems
Exosomes: Therapy delivery tools and biomarkers of diseases
Developing New Delivery Tools for Gene Editing
Gene Transfer Techniques | Journal of Bacteriology
Identification of Genes of VSH-1, a Prophage-Like Gene Transfer Agent of Brachyspira hyodysenteriae Eric G. Matson, M. Greg ... Gene Transfer between Related Bacteria by Electrotransformation: Mapping Salmonella typhi Genes in Salmonella typhimurium ... Gene Transfer and Genome Plasticity in Thermotoga maritima, a Model Hyperthermophilic Species Emmanuel F. Mongodin, Ioana R. ... Role of Agrobacterium virB Genes in Transfer of T Complexes and RSF1010 Karla Jean Fullner ...
The Impact of Gene Transfer Techniques in Eukaryotic Cell Biology | SpringerLink
The Impact of Gene Transfer Techniques in Eukaryotic CeU Biology brought together a number of speakers interested in various ... The 35th N:osbach Colloquium "The Impact of Gene Transfer Techniques in Eukaryotic CeU Biology" brought together a number of ... Genes Involved in Resistance Reactions in Higher Plants: Possible Candidates for Gene Transfer? ... An Alternative Gene Cloning Method for the Isolation of Human Genes by Expression in Mouse Cell Clones ...
WikiGenes - Gene Transfer Techniques
Associations of Gene Transfer Techniques with chemical compounds. *Novel and effective gene transfer technique for study of ... Anatomical context of Gene Transfer Techniques. *We thus employed the adenovirus-mediated gene transfer technique, and we ... Psychiatry related information on Gene Transfer Techniques. *Furthermore, gene transfer techniques using recombinant ... using gene transfer technique [3].. *DNA-mediated gene transfer techniques have been used to study the effectiveness of a novel ...
In vivo nasal potential difference: techniques and protocols for assessing efficacy of gene transfer in cystic fibrosis. -...
Hum Gene Ther. 1995 Apr;6(4):445-55. Comparative Study; Research Support, Non-U.S. Govt; Research Support, U.S. Govt, P.H.S. ... the airway epithelium of CF patients has been targeted for studies of gene transfer. The biological efficacy of gene transfer ... In vivo nasal potential difference: techniques and protocols for assessing efficacy of gene transfer in cystic fibrosis.. ... and can yield estimates of the biological efficacy of gene transfer to achieve correction of the electrolyte transport defects ...
NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.(Statistical Data Included) - Bulletin...
NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.(Statistical Data Included). ... ADAMO, JOSEPH A.; MICHAEL GEALT,. "NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.( ... ADAMO, JOSEPH A.; MICHAEL GEALT,. "NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.( ... ADAMO, JOSEPH A.; MICHAEL GEALT,. "NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.( ...
Gene Therapy in Dermatology: Transfer Techniques and Delivery Systems | Premier Research
Gene transfer techniques. There are two primary approaches for gene transfer:. *In vivo gene therapy, where genetic material is ... In this article, we review gene transfer techniques and gene delivery systems that could potentially be used for treating ... Viral vectors for gene delivery. The epidermis was one of the first targets for in vivo experimental gene transfer, where ... Non-viral gene transfer is typically characterized by short-term gene expression and low transfection efficiency, which may be ...
Gene Transfer Technique - Mybiosource Learning Center
Gene Transfer Technique. Introduction Gene transfer technique is used very widely both in basic research and applied biology. ... Gene transfer is one of the key factors in gene therapy, and it is one of the key purposes of the clone. Gene transfer can be ... The gene transfer results can be transient and stable transfection.. Gene therapy can be defined as the deliberate transfer of ... Gene transfer techniques for higher plants and animals are complex and costly. It is generally not done in laboratory scale ...
Horizontal gene transfer - WUR
To test whether these genes have been acquired from bacteria through horizontal gene transfer, firstly, the distribution of ... Techniques *RNA and DNA isolation. *Genome walking and RACE *Bioinformatics analysis. *BAC library construction and screening ... Horizontal gene transfer. The driving force behind the evolution of plant parasitic nematodes?. ... us clues for the function of a gene.. In this way we are able to silence nematode genes that are putative parasitism related, ...
Gene Delivery to Mammalian Cells | SpringerLink
... it is often necessary to deliver genes to specific cells. In Gene Delivery t ... Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, ... Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the ... An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and ...
Gene transfer and antisense nucleic acid techniques<...
... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ... antisense gene-transfer therapy for a form of lung cancer, and gene-transfer therapies for adenosine deaminase deficiency, ...
New therapeutic approaches based on gene transfer techniques - Fingerprint
- Mayo Clinic
Recent Articles | Horizontal Gene Transfer And Genetics & Genomics | The Scientist Magazine®| Page 3
Advancing Techniques Reveal the Brains Impressive Diversity. By Sara B. Linker, Tracy A. Bedrosian, and Fred H. Gage , ... Combined transcription and genome data from multiple tissues in hundreds of human donors reveal links between genotype and gene ... Small trials using younger donors and elderly recipients hint that mesenchymal stem cell transfers might reduce frailty. ... tags: horizontal gene transfer x genetics & genomics x The Scientist. » horizontal gene transfer and genetics & genomics ...
Eukaryotic Gene Transfer and the New Genetics | The American Journal of Tropical Medicine and Hygiene
In this paper I review some of the techniques that can be used for gene transfer, and speculate on how they might be applied to ... First, the gene of interest is purified, usually by cloning in bacteria, and its structure is determined. Second, biochemical ... techniques are used to introduce mutations into the regions of interest. Finally, the normal and mutant genes are introduced ... In this paper I review some of the techniques that can be used for gene transfer, and speculate on how they might be applied to ...
Bacterial survival in the host and in the environment is promoted by horizontal gene transfer of additional metal resistance...
Bacterial survival in the host and in the environment is promoted by horizontal gene transfer of additional metal resistance ... genes at University of Leicester, listed on FindAPhD.com ... Techniques that will be undertaken during the project. ... 2018 A horizontally gene transferred copper resistance locus confers hyper-resistance to antibacterial copper toxicity and ... Bacterial survival in the host and in the environment is promoted by horizontal gene transfer of additional metal resistance ...
Horizontal Gene Transfer
... (or HGT) is the process of an organism acquiring another organisms DNA, usually through absorbing the ... Raman Spectroscopy as a Universal Analytical Technique for Bodily Fluids. Professor Igor K. Lednev ... Liverpool University recently conducted some research on Horizontal Gene Transfer. Sources. *Horizontal Gene Transfer and ... Horizontal Gene Transfer. News-Medical. 21 July 2019. ,https://www.news-medical.net/life-sciences/Horizontal-Gene-Transfer. ...
Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques | BMC Biotechnology ...
Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be ... the most commonly used methods employ replication-defective viral vectors for gene transfer, while physical gene transfer ... Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. ... Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be ...
Gene Therapy in HIV-Positive Patients With Non-Hodgkin's Lymphoma - Full Text View - ClinicalTrials.gov
Gene Transfer Techniques. Additional relevant MeSH terms: Lymphoma. HIV Infections. Lymphoma, Non-Hodgkin. Neoplasms by ... MedlinePlus related topics: Genes and Gene Therapy HIV/AIDS Lymphoma Genetic and Rare Diseases Information Center resources: ... Gene Therapy in HIV-Positive Patients With Non-Hodgkins Lymphoma. The safety and scientific validity of this study is the ... A Phase IIA Study of Comparative Marking Using a Ribozyme Gene and a Neutral Gene. ...
Evaluating the Safety and Effectiveness of Mozobil Mobilization in Adults With Beta-Thalassemia Major - Full Text View -...
Gene Transfer Techniques. Additional relevant MeSH terms: Thalassemia. beta-Thalassemia. Anemia, Hemolytic, Congenital. Anemia ... Gene transfer, achieved by transplantation of the patients own blood stem cells that have been genetically-modified with the ... The first step in developing gene transfer for treatment of thalassemia is to develop a safe and effective method to obtain ... Eventually, high numbers of genetically modified cells will need to be infused into the patient for clinical gene transfer to ...
Current version of study NCT00336362 on ClinicalTrials.gov
Gene Transfer Techniques. Additional relevant MeSH terms:. Layout table for MeSH terms. ... Gene transfer involves obtaining blood stem cells from an individual, adding a normal globin gene to the stem cells, and ... Before gene transfer methods can be attempted in individuals with beta thalassemia major, a safe method of obtaining blood stem ... A long-term goal of study researchers is to develop a gene transfer process as a method of curing beta thalassemia major. ...
BGI Sequencing news: German EHEC strain is a chimera created by horizontal gene transfer - Biology Fortified Inc.
Among 79 children with acute diarrhea, this technique identified 13 (16.4%) with atypical EPEC, four (5%) with EAEC, three (3.8 ... I would point out that some of these genes (and even plasmid) transfers are cases of Lateral Gene Transfer, and not necessarily ... The acquisition of these genes may have occurred through horizontal gene transfer. The analysis further showed that this deadly ... http://en.citizendium.org/wiki/Horizontal_gene_transfer_in_prokaryotes. 1928. Discovery of gene transfer in bacteria started in ...
Gene transfer into CD4+ T lymphocytes: green fluorescent protein-engineered, encephalitogenic T cells illuminate brain...
Gene transfer into CD4+ T lymphocytes: green fluorescent protein-engineered, encephalitogenic T cells illuminate brain ... Gene Transfer Techniques* * Genetic Engineering / methods * Genetic Vectors * Green Fluorescent Proteins * Luminescent Proteins ... Gene transfer into CD4+ T lymphocytes: green fluorescent protein-engineered, encephalitogenic T cells illuminate brain ...
Safety and efficacy of gene transfer for Leber's congenital amaurosis
One form of the disease, LCA2, is caused by mutations in the retinal pigment epithelium-specific 65-kDa protein gene (RPE65). ... Safety and efficacy of gene transfer for Lebers congenital amaurosis N Engl J Med. 2008 May 22;358(21):2240-8. doi: 10.1056/ ... Although the follow-up was very short and normal vision was not achieved, this study provides the basis for further gene ... One form of the disease, LCA2, is caused by mutations in the retinal pigment epithelium-specific 65-kDa protein gene (RPE65). ...
Maurizio Labbate | University of Technology Sydney
Morbidity and mobile genes - Investigating the role of gene transfer in the emergence of pathogenic vibrio ... We combined quantitative PCR and multiplex PCR/reverse line blot techniques to track patterns in the occurrence of 31 AbR genes ... Pathogenic Vibrio species (identification of genes involved in disease, particularly those derived by lateral gene transfer - ... Lateral gene transfer (LGT) impacts on the evolution of prokaryotes in both the short and long-term. The short-term impacts of ...
A Complete Set of Flagellar Genes Acquired by Horizontal Transfer Coexists with the Endogenous Flagellar System in Rhodobacter...
Recombinant DNA techniques.Strain TOP10 was used for standard recombinant DNA techniques. Plasmid DNA was prepared by using a ... sphaeroides flagellar genes, while the genes in the fla1 cluster were likely acquired as the result of a horizontal transfer ... Successful lateral transfer requires codon usage compatibility between foreign genes and recipient genomes. Mol. Biol. Evol. 21 ... Bacterial type III secretion systems are ancient and evolved by multiple horizontal-transfer events. Gene 312 : 151-163. ...
EXTOXNET FAQs - What is Genetic Engineering?
Transgenic Animals: 1st Edition (Paperback) - Routledge
... transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression ... PART II: THE TECHNIQUES FOR GENE TRANSFER. Gene Transfer into Mammal Embryos ... Gene Transfer into Fish and Lower Vertebrates. Gene Transfer into Invertebrates. The Use of ES Cells for Gene Transfer and Gene ... It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant ...
Using a gene transfer technique to study the role of endothelial nitric oxide synthase in neovascularization and myocardial...
Using a gene transfer technique to study the role of endothelial nitric oxide synthase in neovascularization and myocardial ... Gene Transfer Techniques. Neovascularization, Pathologic. Adenoviridae. Coronary Artery Disease Collection MUSC Theses and ... The effects of eNOS gene delivery were also evaluated in a rat model of myocardial infarction. Six weeks after gene transfer, ... Using a gene transfer technique to study the role of endothelial nitric oxide synthase in neovascularization and myocardial ...
A novel in vivo gene transfer technique and in vitro cell based assays for the study of bone loss in musculoskeletal disorders ...
Phys.org - gene transfer
New technique pinpoints milestones in the evolution of bacteria. Bacteria have evolved all manner of adaptations to live in ... Studying the hotbed of horizontal gene transfers. For over 200,000 years, humans and their gut microbiomes have coevolved into ... Microbes in the human body swap genes, even across tissue boundaries: study. Bacteria in the human body are sharing genes with ... Yeasts reach across tree of life to domesticate suite of bacterial genes. An insects gut might seem an inhospitable place to ...
Phys.org - horizontal gene transfer
New technique pinpoints milestones in the evolution of bacteria. Bacteria have evolved all manner of adaptations to live in ... Cross species transfer of genes has driven evolution. Far from just being the product of our parents, University of Adelaide ... New gene transfer rules could help prevent spread of antibiotic resistance. Unlike other organisms, bacteria can take up ... Studying the hotbed of horizontal gene transfers. For over 200,000 years, humans and their gut microbiomes have coevolved into ...
Therapy2
- Doctors for the first time have used a form of gene therapy to restore partial vision in a blind person, according to findings announced Monday. (futuretimeline.net)
- at present [url+http://en.wikipedia.org/wiki/Gene_therapy]gene therapy[/url] as practised / as envisaged is much more viable and has fewer ethical and moral hurdles. (thenakedscientists.com)