Gene transfer techniques. Medical search

The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.

Moving or repositioning patients within their beds, from bed to bed, bed to chair, or otherwise from one posture or surface to another.

DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.

Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.

A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.

Techniques for determining the proximity of molecules based on ENERGY TRANSFER between bioluminescent chromophores and acceptor fluorophores that have overlapping emission and absorption spectra.

The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).

The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.

A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.

Established cell cultures that have the potential to propagate indefinitely.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.

The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.

Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.

A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.

Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).

Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.

A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

The relationships of groups of organisms as reflected by their genetic makeup.

Change in learning in one situation due to prior learning in another situation. The transfer can be positive (with second learning improved by first) or negative (where the reverse holds).

A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/7311)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors. (+info)

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (2/7311)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis. (+info)

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (3/7311)

Herpes simplex virus type 1 (HSV-1) has many attractive features that suggest its utility for gene transfer to neurons. However, viral cytotoxicity and transient transgene expression limit practical applications even in the absence of viral replication. Mutant viruses deleted for the immediate early (IE) gene, ICP4, an essential transcriptional transactivator, are toxic to many cell types in culture in which only the remaining IE genes are expressed. In order to test directly the toxicity of other IE gene products in neurons and develop a mutant background capable of longterm transgene expression, we generated mutants deleted for multiple IE genes in various combinations and tested their relative cytotoxicity in 9L rat gliosarcoma cells, Vero monkey kidney cells, and primary rat cortical and dorsal root neurons in culture. Viral mutants deleted simultaneously for the IE genes encoding ICP4, ICP22 and ICP27 showed substantially reduced cytotoxicity compared with viruses deleted for ICP4 alone or ICP4 in combination with either ICP22, ICP27 or ICP47. Infection of neurons in culture with these triple IE deletion mutants substantially enhanced cell survival and permitted transgene expression for over 21 days. Such mutants may prove useful for efficient gene transfer and extended transgene expression in neurons in vitro and in vivo. (+info)

Treatment of lysosomal storage disease in MPS VII mice using a recombinant adeno-associated virus. (4/7311)

Mucopolysaccharidosis type VII (MPS VII) is a lysosomal storage disease caused by a genetic deficiency of beta-glucuronidase (GUS). We used a recombinant adeno-associated virus vector (AAV-GUS) to deliver GUS cDNA to MPS VII mice. The route of vector administration had a dramatic effect on the extent and distribution of GUS activity. Intramuscular injection of AAV-GUS resulted in high, localized production of GUS, while intravenous administration produced low GUS activity in several tissues. This latter treatment of MPS VII mice reduced glycosaminoglycan levels in the liver to normal and reduced storage granules dramatically. We show that a single administration of AAV-GUS can provide sustained expression of GUS in a variety of cell types and is sufficient to reverse the disease phenotype at least in the liver. (+info)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (5/7311)

Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain. (+info)

High level inhibition of HIV replication with combination RNA decoys expressed from an HIV-Tat inducible vector. (6/7311)

Intracellular immunization, an antiviral gene therapy approach based on the introduction of DNA into cells to stably express molecules for the inhibition of viral gene expression and replication, has been suggested for inhibition of HIV infection. Since the Tat and Rev proteins play a critical role in HIV regulation, RNA decoys and ribozymes of these sequences have potential as therapeutic molecular inhibitors. In the present study, we have generated several anti-HIV molecules; a tat-ribozyme, RRE, RWZ6 and TAR decoys and combinations of decoys, and tested them for inhibition of HIV-1 replication in vitro. We used T cell specific CD2 gene elements and regulatory the HIV inducible promoter to direct high level expression and a 3' UTR sequence for mRNA stabilization. We show that HIV replication was most strongly inhibited with the combination TAR + RRE decoy when compared with the single decoys or the tat-ribozyme. We also show that the Tat-inducible HIV promoter directs a higher level of steady-state transcription of decoys and inhibitors and that higher levels of expression directly relate to increased levels of inhibition of HIV infection. Furthermore, a stabilization of the 3' end of TAR + RRE inhibitor transcripts using a beta-globin 3' UTR sequence leads to an additional 15-fold increase in steady-state RNA levels. This cassette when used to express the best combination decoy inhibitor TAR + RRE, yields high level HIV inhibition for greater than 3 weeks. Taken together, both optimization for high level expression of molecular inhibitors and use of combinations of inhibitors suggest better therapeutic application in limiting the spread of HIV. (+info)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (7/7311)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity. (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (8/7311)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located. (+info)

... principles and techniques of Gene Transfer and Manipulation. Springer. ISBN 9781461597964. Robert W. Old; Sandy B. Primrose ( ... The gene, once cloned into the cloning vector (called entry clone in this method), may be conveniently introduced into a ... Some techniques employ rolling circle amplification. The most notable of these is described by Smolina et al., 2007 & Smolina ... This adenylyl group is then transferred to the phosphate group at the 5' end of a DNA chain, forming a DNA-adenylate complex. ...

https://en.wikipedia.org/wiki/Ligation_(molecular_biology)

The technique is named immunoprophylaxis by gene transfer (IGT). Animal tests for antibodies to ebola, malaria, influenza, and ... Gene therapy may be classified into two types: In somatic cell gene therapy (SCGT), the therapeutic genes are transferred into ... A new gene therapy approach repaired errors in messenger RNA derived from defective genes. This technique has the potential to ... The first attempt, an unsuccessful one, at gene therapy (as well as the first case of medical transfer of foreign genes into ...

https://en.wikipedia.org/wiki/Gene_therapy

... gene-transfer, and population impacts. Birkhäuser. pp. 163-. ISBN 978-3-7643-5696-5. Retrieved 22 April 2012. William J. ... Sutherland (3 August 2006). Ecological Census Techniques: A Handbook. Cambridge University Press. pp. 202-. ISBN 978-0-521- ...

https://en.wikipedia.org/wiki/Seed_trap

Microbial genetics applied to biotechnology principles and techniques of gene transfer and manipulation. London: Croom Helm. ...

https://en.wikipedia.org/wiki/Streptomyces_vinaceus

Genetically engineered crops have genes added or removed using genetic engineering techniques, originally including gene guns, ... Their natural ability to transfer genes provides another engineering method. To create a suitable environment for themselves, ... The plasmid used to move the gene into soybeans was PV-GMGTO4. It contained three bacterial genes, two CP4 EPSPS genes, and a ... Through tissue culture techniques a single tobacco cell was selected that contained the gene and a new plant grown from it. The ...

https://en.wikipedia.org/wiki/Genetically_modified_crops

Transfer and expression of cloned genes using retroviral vectors. Bio Techniques, 4: 504-512. 1986. Eglitis, M.A.; Kantoff, P ... Persistence and expression of the adenosine deaminase gene for 12 years and immune reaction to gene transfer components: long- ... Gilboa, E.; Anderson, W.F.: Gene expression in mice after high efficiency retroviral-mediated gene transfer. Science, 230: 1395 ... Gene Ther., 2: 215-219, 1991. Anderson, W.F. and Fletcher, J.C.: Gene therapy in human beings: When is it ethical to begin? N. ...

https://en.wikipedia.org/wiki/William_French_Anderson

The Sleeping Beauty transposon system is an example of gene transfer system developed for use in vertebrates. Further ... This technique is used in transgenesis and insertional mutagenesis research fields. ... Current researchers have developed gene transfer systems on the basis of conservative transposition which can integrate new DNA ... The transposase coding region of these transposons is replaced by a gene of interest intended to be integrated into the genome ...

https://en.wikipedia.org/wiki/Conservative_transposition

Currently, he is working on gene transfer techniques to strengthen neurons against the disabling effects of glucocorticoids.[ ... as well as on the possibilities of gene therapy strategies for protecting susceptible neurons from disease. ...

https://en.wikipedia.org/wiki/Robert_Sapolsky

... (SMGT) is a transgenic technique that transfers genes based on the ability of sperm cells to ... one.4 Transgenic animals have been produced successfully using gene transfer techniques such as sperm-mediated gene transfer. ... Gene therapy: the potential applicability of gene transfer technology to the human germline. International Journal of Medical ... Sperm-mediated gene transfer: applications and implications. BioEssays. 27(5):551-562. 5. Collares T, Campos VF, de Leon PM, ...

https://en.wikipedia.org/wiki/Sperm-mediated_gene_transfer

A point of criticism of GMOs is the use of genetic engineering techniques to imprecisely perform horizontal gene transfer. For ... Two types of potato plant varieties are developed using different methods: one using traditional breeding techniques and ... the GM-amylopectin potato only potato DNA from other cultivars is transferred in as a transgene. Some organizations call ... another using genetic manipulation (GMO). Through traditional breeding techniques an amylose-free mutant was obtained without ...

https://en.wikipedia.org/wiki/Waxy_potato_starch

Using gene transfer techniques, researchers in his lab inserted DNA from human bladder tumor cells into normal animal cells. ... 1986 Tumor suppressor gene A tumor suppressor gene, or anti-oncogene, is a gene that protects a cell from one step on the path ... This gene was found to belong to a sub-family of related genes, called ras, that was later discovered to play a role in causing ... When this gene is mutated to cause a loss or reduction in its function, the cell can progress to cancer, usually in combination ...

https://en.wikipedia.org/wiki/Timeline_of_United_States_discoveries

For this purpose, three techniques may be utilized: gene isolation, manipulations, and transferring to target cells. The most ... Ex vivo gene transfer and In-vivo gene transfer. One type of gene therapy in which the gene transfer takes place outside the ... However, in the field of OA gene therapy, researches has more focused on gene transfer as a delivery system for therapeutic ... While viral vectors are 40% more efficient in transferring genes, they are not fully appreciated for in vivo gene delivery ...

https://en.wikipedia.org/wiki/Gene_therapy_for_osteoarthritis

Despite the promising outcomes of the two techniques, pronuclear transfer and spindle transfer, mitochondrial gene replacement ... maternal spindle transfer (MST); pronuclear transfer (PNT); and the newest technique, polar body transfer (PBT). The original ... This technique is used in cases when mothers carry genes for mitochondrial diseases. The therapy is approved for use in the ... Transfer techniques include direct injection into damaged tissue and injection into vessels that supply blood to the tissue. ...

https://en.wikipedia.org/wiki/Mitochondrial_replacement_therapy

... technique used currently because it's a soil pathogenic bacterium that naturally infects plants and transfers their genes (T- ... Once the antigen is identified and isolated, the gene is cloned into a transfer vector. One of the most common transfer vectors ... The gene transfer is independent, and it can express antigens through nuclear and chloroplast transformation. There are a few ... The vector is then turned into the desired strain with the help of the virulence genes of the bacterium. It is then transferred ...

https://en.wikipedia.org/wiki/Edible_vaccine

The Award was given to Dr Bansal for patenting a technique to transfer foreign gene to brinjal chloroplast. It was found that ...

https://en.wikipedia.org/wiki/Rafi_Ahmed_Kidwai_Award

In evolution, viruses are an important means of horizontal gene transfer, which increases genetic diversity. Opinions differ on ... Molecular techniques are used to compare the DNA or RNA of viruses and are a useful means of investigating how they arose. Some ... Viruses are an important natural means of transferring genes between different species, which increases genetic diversity and ... Viruses are an important natural means of transferring genes between different species, which increases genetic diversity and ...

https://en.wikipedia.org/wiki/Marine_viruses

This technique is referred to as oligonucleotide mediated gene repair, targeted gene repair, or targeted nucleotide alteration ... The most common use of lipoplexes has been in gene transfer into cancer cells, where the supplied genes have activated tumor ... Replication-defective vectors always contain a "transfer construct". The transfer construct carries the gene to be transduced ... This is mostly examined for gene transfer in the nervous system. The wild type HSV-1 virus is able to infect neurons and evade ...

https://en.wikipedia.org/wiki/Vectors_in_gene_therapy

"Gene transfer (transfection) methods in animals , Genetic Engineering and Biotechnology Gene Transfer Methods and Transgenic ... Another method is Embryonic Stem Cell-Mediated Gene Transfer. The gene is transfected into embryonic stem cells and then they ... Genetically modified viruses can be used as viral vectors to transfer target genes to another organism in gene therapy. First ... If the gene expresses close homology to a known gene in another species, then it could be isolated by searching for genes in ...

https://en.wikipedia.org/wiki/Genetic_engineering_techniques

Mode of action - therapeutic and immunological medicinal products, gene transfer materials, or cell therapy materials. In the ... It may be prepared using more conventional techniques as is the case for blood or plasma-derived products and a number of ... "based on genes, cells, or tissue engineering", including gene therapy medicines, somatic-cell therapy medicines, tissue- ... Gene-based and cellular biologics, for example, often are at the forefront of biomedicine and biomedical research, and may be ...

https://en.wikipedia.org/wiki/Biopharmaceutical

... participates in the first-ever gene transfer therapy clinical trial in patients with heart failure • Sharp Memorial Hospital's ... transplant team performs the first live donor kidney transplants using laparoscopic technique • The first private employer- ...

https://en.wikipedia.org/wiki/Sharp_Memorial_Hospital

... duplication or horizontal gene transfer. The "genome rearrangement problem", first posed by Watterson and colleagues, asks: ... Such techniques were used by Lemey and colleagues to geographically trace the ancestors of 192 Avian influenza A-H5N1 strains ... comparative genomics and ancestral genome reconstruction has been applied to identify ancient horizontal gene transfer events ... Count reconstructs the evolution of the size of gene families. EREM analyses the gain and loss of genetic features encoded by ...

https://en.wikipedia.org/wiki/Ancestral_reconstruction

The SB system is used primarily in vertebrate animals for gene transfer, including gene therapy, and gene discovery. The ... This means of library preparation is also used in the ATAC-seq technique. The Sleeping Beauty (SB) transposase is the ... June 2009). "Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in ... Genes encoding transposases are widespread in the genomes of most organisms and are the most abundant genes known. During the ...

https://en.wikipedia.org/wiki/Transposase

... and results in the transfer of 'blocks' of genes, i.e. larger segments of chromosomes compared to single genes. R-genes are ... and thus more than one copy of each gene). Various techniques exist to create DHs. The in vitro culture of anthers and ... Many molecular markers can be applied to marker-assisted gene transfer, but the expression of R-genes in the new genetic ... less is known about rye and particularly triticale R-genes. Many R-genes have been transferred to wheat from its wild relatives ...

https://en.wikipedia.org/wiki/Triticale

The vector method is considered a more precise technique than the plasmid method as it involves the transfer of a specific gene ... This technique is used mainly for microorganisms such as bacteria. Through this method, DNA molecules called plasmids are ... Once the vector accepts the genetic code, it is inserted into the host cell where the DNA will be transferred. The biolistic ... In the vector method, a specific gene from a DNA strand is isolated through restriction enzymes in a laboratory and is inserted ...

https://en.wikipedia.org/wiki/Biorobotics

A transgene is a gene that has been transferred naturally, or by any of a number of genetic engineering techniques, from one ... This technique is routinely used to introduce human disease genes or other genes of interest into strains of laboratory mice to ... The transfer of transgenes into the Drosophila genome has been performed using various techniques, including P element, Cre- ... In 1978, yeast cells were the first eukaryotic organisms to undergo gene transfer. Mouse cells were first transformed in 1979, ...

https://en.wikipedia.org/wiki/Transgene

... which Yongsheng Liu and colleagues note could possibly occur by horizontal gene transfer. Isaak Izrailevich Prezent brought ... as well as expanding upon the techniques of vernalization and grafting. In time, the term has come to be identified as any ... They showed that the characteristics of an organism were carried by inherited genes, which were located on chromosomes in each ... Carlson, Elof Axel (1981). Genes, radiation, and society: the life and work of H. J. Muller. Ithaca, NY: Cornell University ...

https://en.wikipedia.org/wiki/Lysenkoism

Under gene transfer conditions, the receptors of interest are coated with the nanoparticles. The receptors will then home in ... Overall, the technique is useful for combatting slow vector accumulation and low vector concentration at target areas. The ... A protocol for NPY gene transfer is being reviewed by the FDA. Somatostatin is a neuropeptide and neuromodulator that plays a ... January 2002). "Magnetofection: enhancing and targeting gene delivery by magnetic force in vitro and in vivo". Gene Therapy. 9 ...

https://en.wikipedia.org/wiki/Gene_therapy_for_epilepsy

Mehier-Humbert S, Guy RH (April 2005). "Physical methods for gene transfer: improving the kinetics of gene delivery into cells ... Researchers have recently used this technique to express reprogramming proteins in primary human fibroblasts. Gene targeting ... Adenoviral vectors can be useful for viral transfection methods because they can transfer genes into a wide variety of human ... Bacchetti S, Graham FL (April 1977). "Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by ...

https://en.wikipedia.org/wiki/Transfection

This results in sequential transfer of genes on the bacterial chromosome. Bacterial geneticists make use of this principle to ... map the genes on the bacterial chromosome. This technique is called interrupted mating as geneticists allow conjugation to take ... In conjugation mediated by Hfr cells, transfer of DNA starts at the origin of transfer (oriT) located within the F factor and ... one can deduce which genes are transferred into the recipient cells first and therefore are closer to the oriT sequence on the ...

https://en.wikipedia.org/wiki/Hfr_cell

A number of techniques are available for inserting the isolated gene into the host genome. Bacteria can be induced to take up ... "GMOs and Horizontal Gene Transfer". NeuroLogica Blog. 13 October 2016. Retrieved 9 July 2021. Zhang C, Wohlhueter R, Zhang H ( ... Some genes are only expressed in certain tissues, so reporter genes, like GUS, can be attached to the gene of interest allowing ... Gene therapy uses genetically modified viruses to deliver genes which can cure disease in humans. Although gene therapy is ...

https://en.wikipedia.org/wiki/Genetically_modified_organism

In this technique, a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to " ... Recently, phage display has also been used in the context of cancer treatments - such as the adoptive cell transfer approach. ... A versatile screening system for selective isolation of genes by specific gene-product/ligand interaction". Eur. J. Biochem. ... Parmley SF, Smith GP (1988). "Antibody-selectable filamentous fd phage vectors: affinity purification of target genes". Gene. ...

https://en.wikipedia.org/wiki/Phage_display

There are no steps, no uniformly appropriate internal attitudes, and no techniques within it. The "health of the helper" is ... a change process developed by philosopher Eugene Gendlin, supports the theory that progress in psychotherapy is dependent on ... that goes beyond a simple transfer of information. ... accessible over the lifespan without stress-relief techniques? ... Health Realization ("HR"), like Sydney Banks's philosophy, is deliberately not taught as a set of "techniques" but as an ...

https://en.wikipedia.org/wiki/Health_realization

Provenzo, Eugene F., Jr. and Jack Thompson. A political odd couple's advice on finding common ground. Christian Science Monitor ... Advertisement placement and other marketing techniques have in the past targeted women as more receptive to non-violent video ... excitation transfer theory, priming effect and the General Aggression Model. However recent scholarship has suggested that ...

https://en.wikipedia.org/wiki/Nonviolent_video_game

Park, Gene (September 17, 2020). "PlayStation CEO Jim Ryan says more PlayStation 5 units will be available than PS4s in 2013". ... In April 2021, Sony released a new software update through which users can transfer their downloaded PS5 game to an external ... The GPU supports hardware-accelerated real-time ray tracing, a rendering technique that allows for realistic lighting and ... Players can synchronize their saved game files through cloud storage or transfer them using a USB storage device so no progress ...

https://en.wikipedia.org/wiki/PlayStation_5

These group-transfer intermediates are called coenzymes. Each class of group-transfer reactions is carried out by a particular ... Using these techniques, a model of human metabolism has now been produced, which will guide future drug discovery and ... Sterck L, Rombauts S, Vandepoele K, Rouzé P, Van de Peer Y (April 2007). "How many genes are there in plants (... and why are ... This reduced form of the coenzyme is then a substrate for any of the reductases in the cell that need to transfer hydrogen ...

https://en.wikipedia.org/wiki/Metabolism

Selosse, M.-A.; Albert, B.; Godelle, B. (2001-03-01). "Reducing the genome size of organelles favours gene transfer to the ... With the emergence of various molecular techniques in the past 50 years, the genome sizes of thousands of eukaryotes have been ... An example for this is the deletion of recF, gene required for the function of recA, and its flanking genes. One of the ... Evidence of the deletion of the function of repair and recombination is the loss of the gene recA, gene involved in the ...

https://en.wikipedia.org/wiki/Genome_size

In 2012, to guarantee a high level of conservation and access, as well as to transfer the considerable financial burden of ... As his son John Lloyd Wright wrote: William Eugene Drummond, Francis Barry Byrne, Walter Burley Griffin, Albert Chase McArthur ... and simplified construction techniques that allowed more mechanization and efficiency in building. Fallingwater, one of ... In his Prairie School days, Wright's office was populated by many talented architects, including William Eugene Drummond, John ...

https://en.wikipedia.org/wiki/Frank_Lloyd_Wright

In 1908, Eugene Sullivan founded glass research also in the United States (Corning, New York). At the beginning of glass ... M. K. Choudhary; R. M. Potter (2005). "9. Heat Transfer in Glass-Forming Melts". In Angelo Montenero; Pye, David; Innocent ... empirical modeling techniques are presented, which seem to be a successful way for handling huge amounts of experimental data. ... ISBN 3-540-57572-3. "Eugene Sullivan and Corning Glass Works". Archived from the original on 2007-10-13. Retrieved 2007-11-05. ...

https://en.wikipedia.org/wiki/Calculation_of_glass_properties

Endosymbiotic gene transfer, the process by which genes that were coded in the mitochondrial genome are transferred to the ... An IVF technique known as mitochondrial donation or mitochondrial replacement therapy (MRT) results in offspring containing ... Adams KL, Palmer JD (December 2003). "Evolution of mitochondrial gene content: gene loss and transfer to the nucleus". ... In the spindle transfer procedure, the nucleus of an egg is inserted into the cytoplasm of an egg from a donor female which has ...

https://en.wikipedia.org/wiki/Mitochondrial_DNA

For example, a technique known as calcium imaging measures the fluorescence intensity of calcium-sensitive dyes to assess ... based gene expression assays, as well as cell viability, cytotoxicity, and biorhythm assays based on the luminescent detection ... time-resolved fluorescence energy transfer). TR-FRET assays are very robust (limited sensitivity to several types of assay ...

https://en.wikipedia.org/wiki/Plate_reader

Kellogg and several other companies wanted to develop techniques for fluid catalytic cracking that would not infringe on Eugene ... In 1943 Landau was invited to transfer to Kellogg's subsidiary, Kellex Corporation, to become head of the chemical department ... In 1955, Scientific Design created another technique, the Mid-Century process of bromine-assisted oxidation of paraxylene to ... Tim Palucka (Winter 2005). "The Wizard of Octane: Eugene Houdry". Invention & Technology. 20 (3). Amos A. Avidan, Michael ...

https://en.wikipedia.org/wiki/Ralph_Landau

Eugene Joseff, who as Joseff of Hollywood and Joseff: Jeweler of the Stars supplied 90 percent of the jewelry used in Hollywood ... July 1 - With the transfer of its assets to the new Military Air Transportation Service completed, the U.S. Navy's Naval Air ... demonstrating the viability of this technique to extend the range of strategic bombers. Two specially modified Gloster Meteors ...

https://en.wikipedia.org/wiki/1948_in_aviation

Mostel had lobbied hard to transfer to Special Services, at one point traveling to Washington to request a transfer. It was not ... His more notable films in these years include the movie version of Rhinoceros (appearing with his Producers costar Gene Wilder ... and he used an acting technique based on muscle memory. He was blacklisted during the 1950s; his testimony before the House Un- ... actor Gene Wilder describes being initially terrified of Mostel. However, just after being introduced, Mostel got up, walked ...

https://en.wikipedia.org/wiki/Zero_Mostel

... the significance of this work is the demonstration that it is possible to effectively transfer a dominant resistance gene from ... That is why it is important to implement control techniques as soon as possible. The disease may be controlled by using genetic ... This method could be expanded to a wide array of genes in the entire family; as with native genes these are best deployed in ... and thus revealed that the number of ASR resistance genes in soybean germplasm is limited[16]. Resistance genes that provide ...

https://en.wikipedia.org/wiki/Phakopsora_pachyrhizi

Dumbo was similar to KIWI/NERVA in concept, but used more advanced construction techniques to lower the weight of the reactor. ... Pulsed reactors attempt to transfer the energy directly from the neutrons to the working mass, allowing the exhaust to reach ... and Eugene Shwageraus; "Detailed Design of 242 m Am Breeding in Pressurized Water Reactors" Nuclear science and engineering ... which would allow for quicker transfer time and increased cargo capacity. The shorter flight duration, estimated at 3-4 months ...

https://en.wikipedia.org/wiki/Nuclear_thermal_rocket

Beside the introduction of the lute to Spain by the Moors, another important point of transfer of the lute from Arabian to ... The name of "pipa" is associated with "tantiao" (彈挑), a right hand techniques of playing a plucked string instrument. "Pi" (琵 ... Eugene Victor (1988). Placeways: A Theory of the Human Environment. Chapel Hill, North Carolina: UNC Press Books. p. 89. ISBN ... Here it takes on the role of the bass instrument using a rhythmical strumming technique. Its physical construction features a ...

https://en.wikipedia.org/wiki/History_of_lute-family_instruments

... to develop techniques allowing the micro-injection of DNA into fertilized eggs as well as the development of gene transfer ... Erwin Wagner's studies are focused in the area of gene function in both healthy and pathological states. His work is focused in ... Wagner obtained his PhD with a thesis on "Regulation of gene expression after virus infection" in 1978. In 1979, Erwin Wagner ... https://www.cnio.es/ES/grupos/plantillas/curriculum.asp?pag=890 "Genes and Disease Group - Erwin Wagner". www.meduniwien.ac.at ...

https://en.wikipedia.org/wiki/Erwin_Friedrich_Wagner

... as the inbreeding first removes many deleterious genes, and permits the expression of genes that allow a population to adapt to ... Inbreeding is a technique used in selective breeding. For example, in livestock breeding, breeders may use inbreeding when ... The goal of preventing the transfer of deleterious alleles may be achieved by reproductive isolation, sterilization, or, in the ... These carriers do not display any signs of the mutation and may be unaware that they carry the mutated gene. Since relatives ...

https://en.wikipedia.org/wiki/Inbreeding

May use gloving techniques similar to the next two classes. Equivalent activity limitation to person with complete cord injury ... Racers don't use a baton, but instead transfer via touch of the body in the exchange zone. The incoming racer cannot use their ... Broekhoff, Jan (1986-06-01). The 1984 Olympic Scientific Congress proceedings: Eugene, Ore., 19-26 July 1984 : (also: OSC ...

https://en.wikipedia.org/wiki/T52_(classification)

The gene that makes the tails of fireflies glow has been added to mustard plants. The plants glow faintly for an hour when ... Morise, H.; Shimomura, O.; Johnson, F.H.; Winant, J. (1974). "Intermolecular energy transfer in the bioluminescent system of ... Luminescence Imaging of Cancer Cells". Metal Ions in Bio-Imaging Techniques. Springer. pp. 371-401. doi:10.1515/9783110685701- ... Koo, J.; Kim, Y.; Kim, J.; Yeom, M.; Lee, I. C.; Nam, H. G. (2007). "A GUS/Luciferase Fusion Reporter for Plant Gene Trapping ...

https://en.wikipedia.org/wiki/Bioluminescence

Although the genetic defect was not known for many years, it was identified in the year 2000 as a mutation in the gene MERTK. ... In October 2003, researchers succeeded in cloning two laboratory rats by nuclear transfer. This was the first in a series of ... giving impetus to the development of genetic research techniques applicable to rat. A 1972 study compared neoplasms in Sprague ... A knockout rat (also spelled knock out or knock-out) is a genetically engineered rat with a single gene turned off through a ...

https://en.wikipedia.org/wiki/Laboratory_rat

The transfer of these loans (along with related risk) to security-buying investors in return for cash replenishes the banks' ... In the fall of 2005 Gene Park, an executive at AIG Financial Products division found, "The percentage of subprime securities in ... Since the introduction of David Li's 2001 model, there have been material advances in techniques that more accurately model ...

https://en.wikipedia.org/wiki/Collateralized_debt_obligation

Furthermore, comparative genomics indicates horizontal gene transfer between Beggiatoa and Cyanobacteria of storage, metabolic ... There are three different possible techniques to obtain isolated Beggiatoa strains in pure culture: Isolation on agar plates ... Pasulka A, Hu SK, Countway PD, Coyne KJ, Cary SC, Heidelberg KB, Caron DA (July 2019). "SSU-rRNA Gene Sequencing Survey of ... ISBN 978-0-387-95592-6. Mukhopadhyaya PN, Deb C, Lahiri C, Roy P (August 2000). "A soxA gene, encoding a diheme cytochrome c, ...

https://en.wikipedia.org/wiki/Beggiatoa

Once the technique is activated, if something is seen as a threat to Ryō through Fried, it will be attacked no matter where the ... He was originally Eugene Currier, a boy who was befriended by the Soul Society scientist Ran'tao before being forced to leave ... But when Yhwach uses his Auswählen on him, knowing his fate is sealed, Haschwalth uses his power to transfer Uryū's wounds onto ... Ganju's earth magic does not seem to operate on the same principles as standard Soul Reaper kidō spells; the one technique he ...

https://en.wikipedia.org/wiki/List_of_Bleach_characters

... it may be involved in horizontal gene transfer; it may provide nutrients; and it may act as a buffer to recruit or titrate ions ... Analytical techniques of eDNA were first applied to terrestrial sediments yielding DNA from both extinct and extant mammals, ... The purified DNA is then amplified for a specific gene target so it can be sequenced and categorised based on its sequence. ... Recent investigations revealed that DNA preserved in marine sediments is characterized by a large number of highly diverse gene ...

https://en.wikipedia.org/wiki/Environmental_DNA

... genes can be synthesized and inserted into organisms or transfected into cells. As of 2002, mice, silkworms, and ... Whole animal imaging (referred to as in vivo when living or, otherwise called ex vivo imaging) is a powerful technique for ... the energy released by the luciferase is instead coupled through resonance energy transfer to the fluorophore of the GFP, and ... Massoud TF, Paulmurugan R, De A, Ray P, Gambhir SS (Feb 2007). "Reporter gene imaging of protein-protein interactions in living ...

https://en.wikipedia.org/wiki/Luciferase

From LST-513, the elevator to transfer equipment between the tank deck and the main deck was replaced with a 12 by 32 ft (3.7 ... "Oral Histories - Exercise Tiger, 28 April 1944 (provided by LT Eugene E. Eckstam, medical officer on USS LST-507)". Naval ... built 145 vessels and developed fabrication techniques that reduced construction time and costs at all of the LST shipyards. ... Following the initial Rotterdam voyage, ASN used their new vessels to transfer thousands of vehicles for the British Army from ...

https://en.wikipedia.org/wiki/Landing_Ship,_Tank

Typically, the gene coding for a protein of interest is fused with the gene for a fluorescent protein, which causes the protein ... This is such a popular technique that it has added a new dimension to the fields of molecular biology, cell biology, and ... "Salute to Innovation" (shtml). UC San Diego Technology Transfer Office (TTO). May 20, 2009. Retrieved May 20, 2009. "HKU to ... Although the specific cause of death was not disclosed, it was reported that he died while on a bike trail in Eugene, Oregon. ...

https://en.wikipedia.org/wiki/Roger_Y._Tsien

Start Over You searched for: Subjects Gene Transfer Techniques ✖Remove constraint Subjects: Gene Transfer Techniques ... 1. Gene transfer in mammalian cells Publication: [Bethesda, Md.] : National Institutes of Health, 1978 Subject(s): ...

https://collections.nlm.nih.gov/?f%5Bdrep2.subjectAggregate%5D%5B%5D=Gene+Transfer+Techniques

Improving Glaucoma Treatment // Experimental Gene Transfer Techniques. Brandt Lab // Featured News // Glaucoma // Kaufman Lab ... discussed the research of experimental gene transfer techniques to improve glaucoma treatment that he and Paul Kaufman, MD, ... Kaufman have collaborated for many years to develop gene delivery methods. Their goal is to deliver a gene into the tissues and ... "That makes it even harder to pick some sort of disease for gene delivery strategy when you can have a little tiny contribution ...

https://www.ophth.wisc.edu/blog/2018/02/14/drs-brandt-kaufman-gene-transfer-research-glaucoma-treatment/

Gene therapy works by altering the genetic code to recover the functions of critical proteins. Discover how this process works. ... If an altered gene causes a necessary protein to be faulty or missing, gene transfer therapy can introduce a normal copy of the ... One such technique uses special structures called nanoparticles as vectors to deliver the genetic material or gene-editing ... Genome editing is a newer technique that may potentially be used for gene therapy. Instead of adding new genetic material, ...

https://medlineplus.gov/genetics/understanding/therapy/procedures/

... transferred ... techniques ... Cell signaling Gene- and Cell-based medicines. ...

https://ki.se/en/labmed/molecular-cell-biology-and-gene-therapy-science

Recently, high frequencies of interspecific gene transfer have been documented, catalyzed by Gene Transfer Agents (GTAs) o … ... Microbial genomic sequence analyses have indicated widespread horizontal gene transfer (HGT). However, an adequate mechanism ... Gene Transfer Techniques Actions. * Search in PubMed * Search in MeSH * Add to Search ... Figure 3. GTA-mediated gene transfer in the reef environment: RnGTA and NrGTA mediated gene transfer frequencies at a site near ...

https://pubmed.ncbi.nlm.nih.gov/22905268

Stereotaxic Techniques Actions. * Search in PubMed * Search in MeSH * Add to Search ... AAV2/1 CD74 Gene Transfer Reduces β-amyloidosis and Improves Learning and Memory in a Mouse Model of Alzheimers Disease Tomomi ... AAV2/1 CD74 Gene Transfer Reduces β-amyloidosis and Improves Learning and Memory in a Mouse Model of Alzheimers Disease Tomomi ... Viral gene transfer of APPsα rescues synaptic failure in an Alzheimers disease mouse model. Fol R, Braudeau J, Ludewig S, Abel ...

https://www.ncbi.nlm.nih.gov/pubmed/?holding=alzped&term=26227349

Gene Transfer Techniques--adverse effects. Genetic Vectors. Patient Selection. Safety Management Publication Types: Congress. ... 3rd Gene Transfer Clinical Research Safety Symposium / National Institutes of Health. Author: Gene Transfer Clinical Research ... Safety Considerations in Cardiovascular Gene Transfer Clinical Research NLM Title: ... 3rd Gene Transfer Clinical Research Safety Symposium ... 3rd Gene Transfer Clinical Research Safety Symposium Your name ...

https://videocast.nih.gov/watch=521

... chemically and physically mediated gene transfer techniques I have come across and a few more!...It is a very useful resource. ... Alphaviruses: Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors. K. Lundstrom. 29. Gene Transfer into Mammalian ... Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual. Subject Area(s): Developmental Biology; Gene ... Delivery of Naked DNA Using Hydrodynamic Injection Techniques. D.L. Lewis, M. Noble, J. Hegge, and J. Wolff. 69. Nonviral Gene ...

https://cshlpress.com/default.tpl?action=full&cart=167592498089273658&--eqskudatarq=562&typ=ps&newtitle=Gene%20Transfer%3A%20Delivery%20and%20Expression%20of%20DNA%20and%20RNA%2C%20A%20Laboratory%20Manual

... chemically and physically mediated gene transfer techniques I have come across and a few more!...It is a very useful resource. ... Alphaviruses: Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors. K. Lundstrom. 29. Gene Transfer into Mammalian ... Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual. Subject Area(s): Developmental Biology; Gene ... Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual [Hardcover]. The Development of Human Gene Therapy [ ...

https://www.cshlpress.com/default.tpl?action=full&cart=1670143524946380974&--eqskudatarq=563&typ=ps&newtitle=Gene%20Transfer%3A%20Delivery%20and%20Expression%20of%20DNA%20and%20RNA%2C%20A%20Laboratory%20Manual

... amphotropic retroviral vectors are widely used for gene transfer into CD34+ hematopoietic progenitor cells. The relatively low ... Gene Transfer Techniques* Actions. * Search in PubMed * Search in MeSH * Add to Search ... High-efficiency gene transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped ... High-efficiency gene transfer into CD34+ cells with a human immunodeficiency virus type 1-based retroviral vector pseudotyped ...

https://pubmed.ncbi.nlm.nih.gov/8642689

Gene Transfer Techniques * Humans * Interleukin-2 / biosynthesis * Membrane Proteins / chemistry* * Membrane Proteins / ... 1 Department of Human Genetics/Medicine, Gene Transfer and Somatic Cell Engineering Laboratory, and Immunology Program, ...

https://pubmed.ncbi.nlm.nih.gov/11753365/?dopt=Abstract

Gene transfer techniques can be used to induce tolerance for beta-cell markers or to alter immune regulatory cells to reduce ... Using gene transfer techniques to blocking apoptotic pathways could produce beta-cells that have a survival advantage after ... Using these gene transfer techniques, beta-cells with new properties can be developed to enhance transplantation. Objectives ... NIDDK and NIAID are soliciting pilot and feasibility grants to explore gene transfer techniques that could be applied to ...

http://grants1.nih.gov/grants/guide/rfa-files/RFA-DK-02-020.html

35 Gene Regulatory Networks -- QU 470 36 Gene Targeting -- QU 550 37 Gene Transfer Techniques -- QU 550 ... At QZ 52, Genetic techniques was changed to Genetic testing.. *At WG 141.5.A3, Angiocardiography. Angioscopy was changed to ... A note was added to QY 95: Classify techniques associated with a specific type of cell with the cell. ...

https://www.nlm.nih.gov/pubs/techbull/mj13/mj13_classification.html

https://grants.nih.gov/grants/guide/rfa-files/RFA-DK-02-020.html

Fluorescent Antibody Technique; Gene Expression Regulation, Enzymologic; Gene Transfer Techniques*; Humans; Leukemia, ... Title: Retroviral mediated gene transfer in megakaryocytic cell lines.. Authors: Kiffmeyer, W R; Stambrook, P J; Lieberman, M A ... with a retroviral vector containing the bacterial lacZ gene and a neomycin resistance marker. Modification of standard ...

https://tools.niehs.nih.gov/portfolio/index.cfm?do=portfolio.publicationDetail&id=751464

2. Lymphocyte apoptosis: induction by gene transfer techniques.. Ebert O; Finke S; Salahi A; Herrmann M; Trojaneck B; Lefterova ... Retrovirus-mediated gene transfer of the cytokine genes interleukin-1beta and tumor necrosis factor-alpha into human ... Intramuscular gene transfer of soluble B7.1/IgG(1) fusion cDNA induces potent antitumor immunity as an adjuvant for DNA ... 3. TNF-alpha secretion and apoptosis of lymphocytes mediated by gene transfer.. Ebert O; Röpke G; Märten A; Lefterova P; Micka ...

https://pubmedhh.nlm.nih.gov/biomarkers/search.php?id=11129282&mod=related&page=1&outid=&proj=

Two groups of investigators have reported independently on their initial observations from Phase I clinical trials of gene ... transfer for Leber congenital amaurosis (LCA) caused by mutations in the RPE65 gene. ... These Phase I clinical trials are designed as a preliminary assessment of the safety of gene transfer techniques in treating ... "Proof-of-concept" studies in animal models of LCA demonstrated gene transfer techniques to be safe, effective, and long-lasting ...

https://www.nei.nih.gov/about/news-and-events/news/gene-transfer-leber-congenital-amaurosis

rDNA techniques: diagnostics. *Gene transfer , genetic modification , microbes, plants, and animals. *Conventional: selection, ... Grafting techniques have existed since 17th century. Propagators grafted plants because it is (or it was) the best method to ... Most nurseries grafting techniques include: T-budding, chip budding, whip and tongue graft, and side veneer graft. I really ... This paper sets out to describe the techniques I used to bring the crop from a breeder?s dream to a venture that generates ...

https://ipps.org/proceedings-content/proceedings-28/Volume-57.html

Confirmed mutation in the DMD gene using a clinically accepted technique that completely defines the mutation ... Phase I Gene Transfer Clinical Trial for Duchenne Muscular Dystrophy Using rAAVrh74.MCK.GALGT2 ... Phase I Gene Transfer Clinical Trial for Duchenne Muscular Dystrophy Using rAAVrh74.MCK.GALGT2 ... Three subjects will receive a single gene transfer dose of 1E12 vector genomes, and patients and investigators will be blinded ...

https://www.niams.nih.gov/grants-funding/phase-i-gene-transfer-clinical-trial-duchenne-muscular-dystrophy-using

Researchers have devised a technique in mice that, with a single injection, protects the immune cells that HIV targets. With ... Researchers have devised a gene transfer technique in mice that, with a single injection, protects the immune cells that HIV ... They modified the virus by inserting the genes that code for an HIV-neutralizing antibody called b12. When the virus was ... The researchers next tested whether the technique could protect against HIV. Mice arent susceptible to HIV, so the researchers ...

https://www.nih.gov/news-events/nih-research-matters/antibodies-protect-against-hiv-mice

These results will expand our understanding of horizontal gene transfer as a bioremediation technique. ... "In addition to completing my project that focuses on gene transfer, I will gain a greater understanding of dechlorinating ... During her externship, Redfern will determine which biogeochemical influences enhance gene transfer between bacterial species ... Nacci at the Narragansett EPA laboratory will allow me to both learn new techniques and skills as well as to establish a ...

https://www.niehs.nih.gov/research/supported/centers/srp/training/donnelly/2015winners/index.cfm

Given the rapid transfer and acquisition of endemic carbapenemase genes, IPC measures are critical to preventing and managing ... 11) were to confirm the outbreak and assess the IPC program, the molecular typing technique used has low discriminatory power ... The gene blaOXA-181 was present in all isolates belonging to ST307. Three isolates carrying the blaOXA-181 gene coharbored the ... of the isolates carried carbapenemase genes. All isolates within ST152 clone harbored the blaNDM-1 gene and contained ColRNAI, ...

https://wwwnc.cdc.gov/eid/article/29/8/23-0484

In the other, these mutations are acquired through gene transfer from neighbouring non-sensitive bacterial species. ... Set vaccines to stun: how a novel technique could help combat antibiotic resistance. Oxford News blog ...

https://www.ox.ac.uk/news/science-blog/set-vaccines-stun-how-novel-technique-could-help-combat-antibiotic-resistance

Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques. Daniels, R., Hall ... wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary ... Cytokine gene expression in peripheral blood mononuclear cells and tissues of cattle infected with Mycobacterium avium subsp. ... Lipopolysaccharide-mediated induction of the bovine interleukin-6 gene in monocytes requires both NF-kappa B and C/EBP binding ...

https://www.wikigenes.org/e/gene/e/280826.html

Bronchoscopic helper-dependent adenovirus delivery in baboons achieved efficient gene transfer and long-term expression. ... Previous gene therapy for cystic fibrosis (CF) was hampered by inefficient gene transfer, as well as by short gene expression ... The technique used a nebulizing catheter with bronchoscopic guidance to deliver vector-containing aerosol sequentially into ... Targeted Delivery Overcomes Many Limitations of Gene Therapy for Cystic Fibrosis. *Authors: News Author: Jacquelyn Beals. CME ...

http://www.medscape.com/viewarticle/565113

... and the ability to mate and transfer an 800- gene genetic code. ... Go programming draws instead on a variety of techniques such as ... Computer gaming is another major leisure field that uses scientific computing techniques, where the programming challenges lie ... Although these applications are proprietary, nevertheless many algorithms and techniques are shared through published papers, ... Computer-generated characters generally use intelligent agents that draw on wider artificial intelligence techniques. ...

https://www.scientific-computing.com/feature/development-through-gaming

With the arrival of recombinant DNA techniques, cloned genes became available and were used to demonstrate that foreign genes ... Efficient retroviral vectors and other gene transfer methods have permitted convincing demonstrations of efficient phenotype ... The concepts of gene therapy arose initially during the 1960s and early 1970s whilst the development of genetically marked ... correction in vitro and in vivo, now making gene therapy a broadly accepted approach to therapy and justifying clinically ...

https://www.nature.com/articles/ng1092-93?error=cookies_not_supported&code=7dce04dc-57eb-41fc-a146-1662e5b230e1

Today, scientists use advanced techniques (like transferring genes or CRISPR) that allow even more precision. But its the same ... When scientists create transgenic crops, they frequently use Agrobacterium to transfer genes from one plant or organism to ... For thousands of years, farmers interbred crops to alter their genes. Like so:. (James Kennedy). In the 20th century, plant ... But nature does this too: scientists recently found that on two separate occasions in history, Agrobacterium transferred ...

https://www.vox.com/2016/6/30/12066826/greenpeace-gmos-nobel-laureates

Techniques for gene transfer between plants that belong to different species or genera fall under the general heading of "wide ... In gene transfer experiments, only a small percentage of the recipient plant cells will actually take up the introduced genes, ... Dale, E. C. and D. W. Ow, "Gene Transfer with Subsequent Removal of the Selection Gene from the Host Genome," Proceedings of ... The kanamycin resistance gene is one of the most widely used selectable marker genes. The kanamycin resistance gene specifies ...

https://www.fda.gov/regulatory-information/search-fda-guidance-documents/statement-policy-foods-derived-new-plant-varieties

Gene transfer may result in genetically transformed cells and individual organisms.. Terms. Gene Transfer Techniques Preferred ... Gene Transfer Techniques Preferred Concept UI. M0027195. Scope Note. The introduction of functional (usually cloned) GENES into ... Gene Delivery Systems Gene Transfer Technique Transgenesis NLM Classification #. QU 550. Previous Indexing. Transfection (1988- ... Gene Transfer Technique Term UI T532462. Date02/11/2003. LexicalTag NON. ThesaurusID NLM (2004). ...

https://meshb.nlm.nih.gov/record/ui?name=Gene+Transfer+Techniques

Gene therapy with viral vectors has been successful, but it does carry some risk. (medlineplus.gov)
One such technique uses special structures called nanoparticles as vectors to deliver the genetic material or gene-editing components into cells. (medlineplus.gov)
Currently, amphotropic retroviral vectors are widely used for gene transfer into CD34+ hematopoietic progenitor cells. (nih.gov)
Efficient retroviral vectors and other gene transfer methods have permitted convincing demonstrations of efficient phenotype correction in vitro and in vivo , now making gene therapy a broadly accepted approach to therapy and justifying clinically applied studies with human patients. (nature.com)
A good portion of the information deals with using viral vectors to get specific gene expression. (virology.net)
There are also references to how these vectors are used in gene therapy. (virology.net)

These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)
When scientists create transgenic crops, they frequently use Agrobacterium to transfer genes from one plant or organism to another. (vox.com)
R gene expression analysis in 18 transgenic events showed different transgenic events exhibiting different levels of expression in the three genes. (academicjournals.org)
However, that pathogen induction and / or high R gene expression are necessary for extreme resistance when transgenic events bear a stack of three R genes was not observed. (academicjournals.org)

With the arrival of recombinant DNA techniques, cloned genes became available and were used to demonstrate that foreign genes could indeed correct genetic defects and disease phenotypes in mammalian cells in vitro . (nature.com)
These methods, including recombinant DNA techniques and cell fusion techniques, enable developers to make genetic modifications in plants, including some modifications that would not be possible with traditional plant breeding methods. (fda.gov)
FDA has received numerous inquiries from industry, government agencies, academia, and the public requesting clarification of the regulatory status of foods, such as fruits, vegetables, grains and their byproducts, derived from new plant varieties developed using recombinant DNA techniques. (fda.gov)

It has been proposed that the presence of bacterial genes in highly purified viral metagenomes may be due to GTAs. (nih.gov)
While working with the potted plants, Lütken and his research team noticed that the transformed plants also had significantly more and longer roots, which sparked a hypothesis: maybe the bacterial genes could help make plants more drought resistant. (hortidaily.com)

Two groups of investigators have reported independently on their initial observations from Phase I clinical trials of gene transfer for Leber congenital amaurosis (LCA) caused by mutations in the RPE65 gene. (nih.gov)
In the other, these mutations are acquired through gene transfer from neighbouring non-sensitive bacterial species. (ox.ac.uk)
Some patients with hereditary hearing loss and dizziness have mutations involving genes critical for inner ear development. (nih.gov)

The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. (cshlpress.com)
1 Department of Human Genetics/Medicine, Gene Transfer and Somatic Cell Engineering Laboratory, and Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. (nih.gov)
For this externship, Buckman will enhance her skills in breeding, growing, and manipulating fish in the laboratory and use these skills to learn about the effects of maternal transfer of methylmercury to their eggs in killifish as a source of early-life methylmercury exposure. (nih.gov)
Working with Dr. Nacci at the Narragansett EPA laboratory will allow me to both learn new techniques and skills as well as to establish a collaborative research project that will take advantage of the facilities, expertise, and support that are available within the Atlantic Ecology Division of the EPA," Buckman said. (nih.gov)
Our laboratory, formerly part of the NIDCD Otolaryngology Surgeon-Scientist Program (OSSP), is focused on developing gene therapy as a treatment option for hearing loss and dizziness. (nih.gov)
Our laboratory is developing ways to delivery gene therapy to the mammalian inner ear in vivo . (nih.gov)
In "Microbes in our intestines may nities and their genes--the "micro- laboratory experiments, transferring play critical roles in how we absorb biome. (nih.gov)

Ex vivo gene transfer approaches could be one method to engineer beta-cells or alter islets to enhance viability that could have advantages for transplantation. (nih.gov)

Gene therapy works by altering the genetic code to recover the functions of critical proteins. (medlineplus.gov)

7. In vitro enhanced cytotoxicity of tumor-infiltrating lymphocytes transfected with tumor necrosis factor-related apoptosis-inducing ligand and/or interleukin-2 gene in human renal cell carcinoma. (nih.gov)

A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION , chromosome-mediated gene transfer, TRANSFECTION , and GENETIC TRANSDUCTION . (nih.gov)

If the treatment is successful, the new gene delivered by the vector will make a functioning protein or the editing molecules will correct a DNA error and restore protein function. (medlineplus.gov)
Bulcha JT, Wang Y, Ma H, Tai PWL, Gao G. Viral vector platforms within the gene therapy landscape. (medlineplus.gov)
A carrier called a vector is genetically engineered to deliver the gene. (medlineplus.gov)
Here we show that a human immunodeficiency virus type 1 (HIV-1)-based retroviral vector containing the firefly luciferase reporter gene can be pseudotyped with a broad-host-range vesicular stomatitis virus envelope glycoprotein G (VSV-G). Higher-efficiency gene transfer into CD34+ cells was achieved with a VSV-G-pseudotyped HIV-1 vector than with a vector packaged in an amphotropic envelope. (nih.gov)
We have successfully infected two megakaryocytic cell lines, one a committed cell line (CHRF-288-11) and the other a bipotential cell line (K562), with a retroviral vector containing the bacterial lacZ gene and a neomycin resistance marker. (nih.gov)
Three subjects will receive a single gene transfer dose of 1E12 vector genomes, and patients and investigators will be blinded as to which muscle is injected with vector. (nih.gov)
Gene Transfer Vector Core - the Gene Transfer Vector Core Page at the University of Iowa is dedicated to gene transfer and vector construction. (virology.net)

GENE TRANSFER APPROACHES TO ENHANCE ISLET TRANSPLANTATION Release Date: September 20, 2001 RFA: RFA-DK-02-020 National Institute of Diabetes and Digestive and Kidney Diseases ( http://www.niddk.nih.gov/ ) National Institute of Allergy and Infectious Diseases ( http://www.niaid.nih.gov/ ) Letter of Intent Receipt Date: November 15, 2001 Application Receipt Date: December 12, 2001 THIS RFA USES "MODULAR GRANT" AND "JUST-IN-TIME" CONCEPTS. (nih.gov)
PURPOSE The National Institute of Diabetes and Digestive and Kidney Diseases and the National Institute of Allergy and Infectious Diseases are soliciting applications to develop gene transfer approaches to enhance islet transplantation. (nih.gov)
NIDDK and NIAID are soliciting pilot and feasibility grants to explore gene transfer techniques that could be applied to enhanced islet transplantation. (nih.gov)
This Request for Applications (RFA), Gene Transfer Approaches to Enhance Islet Transplantation, is related to the priority area of Diabetes and Chronic Disabling Conditions. (nih.gov)
Gene therapy: potential applications in clinical transplantation - This is a review published in the internet journal, Expert Reviews in Molecular Medicine that reviews the latest development of gene transfer in the context of organ transplantation. (virology.net)

In other words, we can find or identify genes that would act on those cells to increase the fluid flow without necessarily actually replacing a gene that happens to have a problem with it or a defect," Dr. Brandt said. (wisc.edu)
Gene transfer therapy introduces new genetic material into cells. (medlineplus.gov)
For example, scientists are finding better ways to deliver genes or gene-editing tools and target them to particular cells. (medlineplus.gov)
Cells constantly receive signals from the outer world and such information is transferred through receptors and via biochemical pathways into the nucleus. (ki.se)
Understanding gene function and regulation requires rigorous testing in live cells and organisms. (cshlpress.com)
Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
4. Increase of proliferation rate and enhancement of antitumor cytotoxicity of expanded human CD3+ CD56+ immunologic effector cells by receptor-mediated transfection with the interleukin-7 gene. (nih.gov)
5. Effects of adenoviral wild-type p53 gene transfer in p53-mutated lymphoma cells. (nih.gov)
9. Engineered CD8+ cytotoxic T cells with fiber-modified adenovirus-mediated TNF-alpha gene transfection counteract immunosuppressive interleukin-10-secreting lung metastasis and solid tumors. (nih.gov)
15. Retrovirus-mediated gene transfer of the cytokine genes interleukin-1beta and tumor necrosis factor-alpha into human neuroblastoma cells: consequences for cell line behavior and immunomodulatory properties. (nih.gov)
Researchers have devised a gene transfer technique in mice that, with a single injection, protects the immune cells that HIV targets. (nih.gov)
The concepts of gene therapy arose initially during the 1960s and early 1970s whilst the development of genetically marked cells lines and the clarification of mechanisms of cell transformation by the papovaviruses polyoma and SV40 was in progress. (nature.com)
The introduction of functional (usually cloned) GENES into cells. (nih.gov)
Gene transfer may result in genetically transformed cells and individual organisms. (nih.gov)

In particular, a unique method of intra-arterial gene transfer under vascular isolation that results in highly efficient and localized transgene expression will be used for gene transfer and laser Doppler perfusion imaging to measure dermal blood flow will facilitate the accomplishment of the above aims. (nih.gov)

Durability of effects is crucial given the history of human gene therapy trials which have demonstrated that early gene expression can be thwarted by immunologic or other responses. (nih.gov)

Centre for Gene Therapeutics - McMaster University - Investigating, creating and implementing approaches utilizing the delivery of genes as therapeutic agents in the treatment of human and animal disease. (virology.net)

But nature does this too: scientists recently found that on two separate occasions in history, Agrobacterium transferred bacterial DNA into the sweet potatoes we eat. (vox.com)
New techniques have allowed scientists to more precisely alter animal genomes by editing DNA to include or exclude beneficial or harmful traits. (bostonglobe.com)
New techniques allow scientists to your gut can affect how well you needed to confirm this connection. (nih.gov)

These Phase I clinical trials are designed as a preliminary assessment of the safety of gene transfer techniques in treating people with LCA. (nih.gov)
These insights will enable investigators to refine the gene transfer techniques and will provide the preliminary information needed to design the Phase II and Phase III clinical trials that are necessary to determine both safety and effectiveness in a rigorous way. (nih.gov)
Gene therapy clinical trials website from the Journal of Gene Medicine. (virology.net)

Their goal is to deliver a gene into the tissues and get a therapeutic effect that will last for a long time. (wisc.edu)

Dr. Brandt said there is significant evidence that glaucoma is a multifactorial disease, thus it is likely the result of a combination of different genes people can inherit. (wisc.edu)
That makes it even harder to pick some sort of disease for gene delivery strategy when you can have a little tiny contribution from ten different genes that eventually combine and lead to the disease," Dr. Brandt said. (wisc.edu)
No conclusions can be made at present as to whether gene transfer will restore visual function in people with LCA or impede further progression of the disease. (nih.gov)
Using techniques as diverse as exome sequencing, gene profiling and tissue culture, these studies have already revealed unique disease-specific genetic and protein changes. (stanford.edu)

This article is intended for clinicians who want to maintain a current understanding of recent research and evidence in gene therapy delivery in cystic fibrosis. (medscape.com)
Inform clinicians of the latest medical information on bronchoscopic delivery of gene therapy in patients with cystic fibrosis, as reported at the American Society of Human Genetics 57th Annual Meeting. (medscape.com)
Oxford University GeneMedicine - Cystic fibrosis gene therapy at Oxford University. (virology.net)

This program enables investigators to explore the feasibility of a concept related to gene therapy of diabetes and generate sufficient data to pursue it through other funding mechanisms. (nih.gov)
2) allow exploration of possible innovative new leads or new directions for established investigators in gene therapy and (3) stimulate investigators from other areas to lend their expertise to research in this area. (nih.gov)
A number of DAB-supported investigators are seeking to establish companies, some supported by NIA Small Business Innovative Research (SBIR) and Small Business Technology Transfer (STTR) programs, to translate their scientific findings to practical ways of improving health in the human population. (nih.gov)

We are currently testing whether the delivery of normal copies of the mutated genes into animal models of hereditary hearing loss can restore hearing and balance. (nih.gov)
This can potentially be used to correct the mutation in the affected genes and restore normal function. (nih.gov)
We are currently studying whether genome editing can be used as a form of gene therapy to restore hearing and balance in animal models. (nih.gov)

Verma, I.M. Gene therapy. (nature.com)

the world, and increased calories and Humans need bacteria and their decreased exercise seem insufficient newsinhealth.nih.gov genes more than most of us thought. (nih.gov)

2. Lymphocyte apoptosis: induction by gene transfer techniques. (nih.gov)

Microbial genomic sequence analyses have indicated widespread horizontal gene transfer (HGT). (nih.gov)

They modified the virus by inserting the genes that code for an HIV-neutralizing antibody called b12. (nih.gov)

Nichols, E.K. Gene therapy . (nature.com)

Biotechnology and Gene Therapy Web 'Cite' - a large index of Gene Therapy information on the net. (virology.net)

What they have in common is that they received new genes and traits from Rhizobium rhizogenes, a bacterium with the special ability to transfer its genes into host plants, and by doing so, transforming them. (hortidaily.com)
Conventionally, chemical growth inhibitors have been used to achieve the same results, but with the help of this bacterium and its genes, we have developed plants in which these traits are inherent. (hortidaily.com)

Concentration of virus without loss of viral infectivity permitted a higher multiplicity of infection, with a consequent higher efficiency of gene transfer, reaching 2.8 copies per cell. (nih.gov)

This incredible bacterium can insert some of its genes into plants in a process called transformation. (hortidaily.com)

Alternatively, the therapy can introduce a different gene that provides instructions for a protein that helps the cell function normally, despite the genetic alteration. (medlineplus.gov)
Instead of adding new genetic material, genome editing introduces gene-editing tools that can change the existing DNA in the cell. (medlineplus.gov)
Genetic material or gene-editing tools that are inserted directly into a cell usually do not function. (medlineplus.gov)
Some types of virus, such as retroviruses, integrate their genetic material (including the new gene) into a chromosome in the human cell. (medlineplus.gov)
Viruses can also deliver the gene-editing tools to the nucleus of the cell. (medlineplus.gov)
For gene therapy, these tiny particles are designed with specific characteristics to target them to particular cell types. (medlineplus.gov)
A new gene is inserted directly into a cell. (medlineplus.gov)
Our research group, under the lead of Edvard Smith and Rula Zain-Luqman, has two main programs: Cell signaling Gene- and Cell-based medicines. (ki.se)
1. Gene and Cell therapy. (ki.se)
Retroviral mediated gene transfer in megakaryocytic cell lines. (nih.gov)
Center for Cell and Gene Therapy - Description of the Center Cell and Gene Therapy (Baylor College of Medecine / Texas Children's Hospital / The Methodist Hospital) located in the Texas Medical Center, Houston, Texas. (virology.net)
The Nolan Lab - description and tutorials on the use of human cell-basedhelper-defective cell lines for efficient gene transfer. (virology.net)
Moffitt Cancer Center currently offers several types of immunotherapy for cancer treatment, including chimeric antigen receptor (CAR) T-cell therapy, T-cell receptor (TCR) gene transfers and tumor-infiltrating lymphocyte (TIL) therapy. (moffitt.org)

Dr. Brandt and Dr. Kaufman have collaborated for many years to develop gene delivery methods. (wisc.edu)

Anderson, W.F. Prospects for human gene therapy. (nature.com)
Friedmann, T. Progress toward human gene therapy. (nature.com)
Anderson, W.F. Human gene therapy. (nature.com)
Miller, A.D. Human gene therapy comes of age. (nature.com)

Researchers are developing newer technologies that can deliver genetic material or gene-editing tools without using viruses. (medlineplus.gov)

In an interview with WYPL-FM's (Memphis, TN) radio show Eye on Vision , Curtis Brandt, PhD, professor of ophthalmology and visual sciences, discussed the research of experimental gene transfer techniques to improve glaucoma treatment that he and Paul Kaufman, MD, perform here at the University of Wisconsin. (wisc.edu)
This research program is related to the development of new gene medicines mainly based on synthetic oligo-nucleotides (ONs). (ki.se)
Our research aims at developing molecules that can specifically turn off a gene, or correct the production of a miss-spliced mRNA. (ki.se)
The research also involves studies on the cellular uptake of gene medicines. (ki.se)
Report and Recommendations of the Panel to Assess the NIH Investment in Research on Gene Therapy - Dr. Harold Varmus, Director, National Institutes of Health (NIH), appointed an ad hoc committee to assess the current status and promise of gene therapy and provide recommendations regarding future NIH-sponsored research in this area. (virology.net)
University of North Carolina Gene Therapy Center - Links to current gene therapy research at University of North Carolina as well as contacts to individual researchers. (virology.net)

The initial observations indicate that within a short follow-up period, five months to one year, gene transfer for LCA appears to be well-tolerated and safe. (nih.gov)
Nonetheless, several of the reported observations suggest that gene transfer may be exerting modest effects in some of the treated individuals. (nih.gov)

Go programming draws instead on a variety of techniques such as pattern recognition, cellular automata and combinatorial game theory. (scientific-computing.com)

Summary: The Food and Drug Administration (FDA) is issuing a policy statement on foods derived from new plant varieties, including plants developed by recombinant deoxyribonucleic acid (DNA) techniques. (fda.gov)

Researchers continue to work to overcome the many technical challenges of gene therapy. (medlineplus.gov)
The researchers next tested whether the technique could protect against HIV. (nih.gov)
Researchers are now experimenting with modifying the genes of chickens so they don't transfer avian flu, for example. (bostonglobe.com)
Now, researchers at the University of Copenhagen are taking a natural shortcut to promote beneficial changes using a special bacterium that can transfer its genes into plants. (hortidaily.com)

Recently, high frequencies of interspecific gene transfer have been documented, catalyzed by Gene Transfer Agents (GTAs) of marine α-Proteobacteria. (nih.gov)

Proof-of-concept" studies in animal models of LCA demonstrated gene transfer techniques to be safe, effective, and long-lasting in restoring visual function. (nih.gov)

Introduction to Gene Therapy - Transfer Technology - This is a site being made for a course taught at Vanderbilt University for graduate and medical students. (virology.net)

Gene Mediated Cytotoxic Immunotherapy (GMCI) is based on an innovative technique known as gene transfer technology and is used in conjunction with current therapies for a targeted approach. (houstonmethodist.org)

Fletcher's technique of inserting growth hormone from Chinook salmon and a "promoter gene" from ocean pout is now considered antiquated technology. (bostonglobe.com)

Anatomy2

Organisms3

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Psychiatry and Psychology1

Phenomena and Processes11

Information Science2

Health Care1

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/7311)

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (2/7311)

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (3/7311)

Treatment of lysosomal storage disease in MPS VII mice using a recombinant adeno-associated virus. (4/7311)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (5/7311)

High level inhibition of HIV replication with combination RNA decoys expressed from an HIV-Tat inducible vector. (6/7311)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (7/7311)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (8/7311)

Gene Transfer Techniques

Moving and Lifting Patients

Genetic Vectors

Genetic Therapy

Transfection

Embryo Transfer

Fluorescence Resonance Energy Transfer

Bioluminescence Resonance Energy Transfer Techniques

Gene Transfer, Horizontal

Energy Transfer

Adenoviridae

Cell Line

Cells, Cultured

Pregnancy

Transduction, Genetic

Nuclear Transfer Techniques

Dependovirus

Retroviridae

Transgenes

beta-Galactosidase

Gene Expression

Plasmids

Phylogeny

Transfer (Psychology)

Electroporation

Molecular Sequence Data

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/7311)

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (2/7311)

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (3/7311)

Treatment of lysosomal storage disease in MPS VII mice using a recombinant adeno-associated virus. (4/7311)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (5/7311)

High level inhibition of HIV replication with combination RNA decoys expressed from an HIV-Tat inducible vector. (6/7311)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (7/7311)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (8/7311)

Ligation (molecular biology)

Gene therapy

Seed trap

Streptomyces vinaceus

Genetically modified crops

William French Anderson

Conservative transposition

Robert Sapolsky

Sperm-mediated gene transfer

Waxy potato starch

Timeline of United States discoveries

Gene therapy for osteoarthritis

Mitochondrial replacement therapy

Edible vaccine

Rafi Ahmed Kidwai Award

Marine viruses

Vectors in gene therapy

Genetic engineering techniques

Biopharmaceutical

Sharp Memorial Hospital

Ancestral reconstruction

Transposase

Triticale

Biorobotics

Transgene

Lysenkoism

Gene therapy for epilepsy

Transfection

Hfr cell

Genetically modified organism

Phage display

Health realization

Nonviolent video game

PlayStation 5

Metabolism

Genome size

Frank Lloyd Wright

Calculation of glass properties

Mitochondrial DNA

Plate reader

Ralph Landau

1948 in aviation

Zero Mostel

Phakopsora pachyrhizi

Nuclear thermal rocket

History of lute-family instruments