AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesInformation ScienceHealth Care
Gene Transfer TechniquesMoving and Lifting PatientsGenetic VectorsGenetic TherapyTransfectionEmbryo TransferFluorescence Resonance Energy TransferBioluminescence Resonance Energy Transfer TechniquesGene Transfer, HorizontalEnergy TransferAdenoviridaeCell LineCells, CulturedPregnancyTransduction, GeneticNuclear Transfer TechniquesDependovirusRetroviridaeTransgenesbeta-GalactosidaseGene ExpressionPlasmidsPhylogenyTransfer (Psychology)ElectroporationMolecular Sequence Data
Gene Transfer Techniques: The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.Moving and Lifting Patients: Moving or repositioning patients within their beds, from bed to bed, bed to chair, or otherwise from one posture or surface to another.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Genetic Therapy: Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Embryo Transfer: The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.Fluorescence Resonance Energy Transfer: A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.Bioluminescence Resonance Energy Transfer Techniques: Techniques for determining the proximity of molecules based on ENERGY TRANSFER between bioluminescent chromophores and acceptor fluorophores that have overlapping emission and absorption spectra.Gene Transfer, Horizontal: The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).Energy Transfer: The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.Adenoviridae: A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Transduction, Genetic: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.Nuclear Transfer Techniques: Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.Dependovirus: A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.Retroviridae: Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).Transgenes: Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Transfer (Psychology): Change in learning in one situation due to prior learning in another situation. The transfer can be positive (with second learning improved by first) or negative (where the reverse holds).Electroporation: A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (1/7311)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Prevention of collagen-induced arthritis by gene delivery of soluble p75 tumour necrosis factor receptor. (2/7311)

Collagen type II-induced arthritis (CIA) in DBA/1 mice can be passively transferred to SCID mice with spleen B- and T-lymphocytes. In the present study, we show that infection ex vivo of splenocytes from arthritic DBA/1 mice with a retroviral vector, containing cDNA for the soluble form of human p75 receptor of tumour necrosis factor (TNF-R) before transfer, prevents the development of arthritis, bone erosion and joint inflammation in the SCID recipients. Assessment of IgG subclass levels and studies of synovial histology suggest that down-regulating the effector functions of T helper-type 1 (Th1) cells may, at least in part, explain the inhibition of arthritis in the SCID recipients. In contrast, the transfer of splenocytes infected with mouse TNF-alpha gene construct resulted in exacerbated arthritis and enhancement of IgG2a antibody levels. Intriguingly, infection of splenocytes from arthritic DBA/1 mice with a construct for mouse IL-10 had no modulating effect on the transfer of arthritis. The data suggest that manipulation of the immune system with cytokines, or cytokine inhibitors using gene transfer protocols can be an effective approach to ameliorate arthritis.  (+info)

Deletion of multiple immediate-early genes from herpes simplex virus reduces cytotoxicity and permits long-term gene expression in neurons. (3/7311)

Herpes simplex virus type 1 (HSV-1) has many attractive features that suggest its utility for gene transfer to neurons. However, viral cytotoxicity and transient transgene expression limit practical applications even in the absence of viral replication. Mutant viruses deleted for the immediate early (IE) gene, ICP4, an essential transcriptional transactivator, are toxic to many cell types in culture in which only the remaining IE genes are expressed. In order to test directly the toxicity of other IE gene products in neurons and develop a mutant background capable of longterm transgene expression, we generated mutants deleted for multiple IE genes in various combinations and tested their relative cytotoxicity in 9L rat gliosarcoma cells, Vero monkey kidney cells, and primary rat cortical and dorsal root neurons in culture. Viral mutants deleted simultaneously for the IE genes encoding ICP4, ICP22 and ICP27 showed substantially reduced cytotoxicity compared with viruses deleted for ICP4 alone or ICP4 in combination with either ICP22, ICP27 or ICP47. Infection of neurons in culture with these triple IE deletion mutants substantially enhanced cell survival and permitted transgene expression for over 21 days. Such mutants may prove useful for efficient gene transfer and extended transgene expression in neurons in vitro and in vivo.  (+info)

Treatment of lysosomal storage disease in MPS VII mice using a recombinant adeno-associated virus. (4/7311)

Mucopolysaccharidosis type VII (MPS VII) is a lysosomal storage disease caused by a genetic deficiency of beta-glucuronidase (GUS). We used a recombinant adeno-associated virus vector (AAV-GUS) to deliver GUS cDNA to MPS VII mice. The route of vector administration had a dramatic effect on the extent and distribution of GUS activity. Intramuscular injection of AAV-GUS resulted in high, localized production of GUS, while intravenous administration produced low GUS activity in several tissues. This latter treatment of MPS VII mice reduced glycosaminoglycan levels in the liver to normal and reduced storage granules dramatically. We show that a single administration of AAV-GUS can provide sustained expression of GUS in a variety of cell types and is sufficient to reverse the disease phenotype at least in the liver.  (+info)

Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism. (5/7311)

Parkinson's disease is a neurodegenerative disorder characterized by the depletion of dopamine in the caudate putamen. Dopamine replacement with levodopa, a precursor of the neurotransmitter, is presently the most common treatment for this disease. However, in an effort to obtain better therapeutic results, tissue or cells that synthesize catecholamines have been grafted into experimental animals and human patients. In this paper, we present a novel technique to express tyrosine hydroxylase (TH) in the host's own astrocytes. This procedure uses a transgene in which the expression of a TH cDNA is under the control of a glial fibrillary acidic protein (GFAP) promoter, which confers astrocyte-specific expression and also increases its activity in response to brain injury. The method was tested in a rat model of Parkinson's disease produced by lesioning the striatum with 6-hydroxydopamine. Following microinjection of the transgene into the denervated striatum as a DNA-liposome complex, expression of the transgene was detected by RT-PCR and TH protein was observed specifically in astrocytes by using double-labeling immunofluorescence for GFAP and TH coupled with laser confocal microscopy. Efficacy was demonstrated by significant behavioral recovery, as assessed by a decrease in the pharmacologically induced turning behavior generated by the unilateral denervation of the rat striatum. These results suggest this is a valuable technique to express molecules of therapeutic interest in the brain.  (+info)

High level inhibition of HIV replication with combination RNA decoys expressed from an HIV-Tat inducible vector. (6/7311)

Intracellular immunization, an antiviral gene therapy approach based on the introduction of DNA into cells to stably express molecules for the inhibition of viral gene expression and replication, has been suggested for inhibition of HIV infection. Since the Tat and Rev proteins play a critical role in HIV regulation, RNA decoys and ribozymes of these sequences have potential as therapeutic molecular inhibitors. In the present study, we have generated several anti-HIV molecules; a tat-ribozyme, RRE, RWZ6 and TAR decoys and combinations of decoys, and tested them for inhibition of HIV-1 replication in vitro. We used T cell specific CD2 gene elements and regulatory the HIV inducible promoter to direct high level expression and a 3' UTR sequence for mRNA stabilization. We show that HIV replication was most strongly inhibited with the combination TAR + RRE decoy when compared with the single decoys or the tat-ribozyme. We also show that the Tat-inducible HIV promoter directs a higher level of steady-state transcription of decoys and inhibitors and that higher levels of expression directly relate to increased levels of inhibition of HIV infection. Furthermore, a stabilization of the 3' end of TAR + RRE inhibitor transcripts using a beta-globin 3' UTR sequence leads to an additional 15-fold increase in steady-state RNA levels. This cassette when used to express the best combination decoy inhibitor TAR + RRE, yields high level HIV inhibition for greater than 3 weeks. Taken together, both optimization for high level expression of molecular inhibitors and use of combinations of inhibitors suggest better therapeutic application in limiting the spread of HIV.  (+info)

Thyroid hormone effects on Krox-24 transcription in the post-natal mouse brain are developmentally regulated but are not correlated with mitosis. (7/7311)

Krox-24 (NGFI-A, Egr-1) is an immediate-early gene encoding a zinc finger transcription factor. As Krox-24 is expressed in brain areas showing post-natal neurogenesis during a thyroid hormone (T3)-sensitive period, we followed T3 effects on Krox-24 expression in newborn mice. We analysed whether regulation was associated with changes in mitotic activity in the subventricular zone and the cerebellum. In vivo T3-dependent Krox-24 transcription was studied by polyethylenimine-based gene transfer. T3 increased transcription from the Krox-24 promoter in both areas studied at post-natal day 2, but was without effect at day 6. An intact thyroid hormone response element (TRE) in the Krox-24 promoter was necessary for these inductions. These stage-dependent effects were also seen in endogenous Krox-24 mRNA levels: activation at day 2 and no effect at day 6. Moreover, similar results were obtained by examining beta-galactosidase expression in heterozygous mice in which one allele of the Krox-24 gene was disrupted with an inframe Lac-Z insertion. However, bromodeoxyuridine incorporation showed mitosis to continue through to day 6. We conclude first, that T3 activates Krox-24 transcription during early post-natal mitosis but that this effect is extinguished as development proceeds and second, loss of T3-dependent Krox-24 expression is not correlated with loss of mitotic activity.  (+info)

Reversal of hyperlipidaemia in apolipoprotein C1 transgenic mice by adenovirus-mediated gene delivery of the low-density-lipoprotein receptor, but not by the very-low-density-lipoprotein receptor. (8/7311)

We have shown previously that human apolipoprotein (apo)C1 transgenic mice exhibit hyperlipidaemia, due primarily to an impaired clearance of very-low-density lipoprotein (VLDL) particles from the circulation. In the absence of at least the low-density-lipoprotein receptor (LDLR), it was shown that APOC1 overexpression in transgenic mice inhibited the hepatic uptake of VLDL via the LDLR-related protein. In the present study, we have now examined the effect of apoC1 on the binding of lipoproteins to both the VLDL receptor (VLDLR) and the LDLR. The binding specificity of the VLDLR and LDLR for apoC1-enriched lipoprotein particles was examined in vivo through adenovirus-mediated gene transfer of the VLDLR and the LDLR [giving rise to adenovirus-containing (Ad)-VLDLR and Ad-LDLR respectively] in APOC1 transgenic mice, LDLR-deficient (LDLR-/-) mice and wild-type mice. Remarkably, Ad-VLDLR treatment did not reduce hyperlipidaemia in transgenic mice overexpressing human APOC1, irrespective of both the level of transgenic expression and the presence of the LDLR, whereas Ad-VLDLR treatment did reverse hyperlipidaemia in LDLR-/- and wild-type mice. On the other hand, Ad-LDLR treatment strongly decreased plasma lipid levels in these APOC1 transgenic mice. These results suggest that apoC1 inhibits the clearance of lipoprotein particles via the VLDLR, but not via the LDLR. This hypothesis is corroborated by in vitro binding studies. Chinese hamster ovary (CHO) cells expressing the VLDLR (CHO-VLDLR) or LDLR (CHO-LDLR) bound less APOC1 transgenic VLDL than wild-type VLDL. Intriguingly, however, enrichment with apoE enhanced dose-dependently the binding of wild-type VLDL to CHO-VLDLR cells (up to 5-fold), whereas apoE did not enhance the binding of APOC1 transgenic VLDL to these cells. In contrast, for binding to CHO-LDLR cells, both wild-type and APOC1 transgenic VLDL were stimulated upon enrichment with apoE. From these studies, we conclude that apoC1 specifically inhibits the apoE-mediated binding of triacylglycerol-rich lipoprotein particles to the VLDLR, whereas apoC1-enriched lipoproteins can still bind to the LDLR. The variability in specificity of these lipoprotein receptors for apoC1-containing lipoprotein particles provides further evidence for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located.  (+info)

Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer. | BMC Biotechnology | Full TextEffective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer. | BMC Biotechnology | Full Text
Transgenic animals have become valuable tools for both research and applied purposes. The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. Here, we report a linker based sperm-mediated gene transfer method (LB-SMGT) that greatly improves the production efficiency of large transgenic animals. The linker protein, a monoclonal antibody (mAb C), is reactive to a surface antigen on sperm of all tested species including pig, mouse, chicken, cow, goat, sheep, and human. mAb C is a basic protein that binds to DNA through ionic interaction allowing exogenous DNA to be linked specifically to sperm. After fertilization of the egg, the DNA is shown to be successfully integrated into the genome of viable pig and mouse offspring with germ-line transfer to the F1 generation at a highly efficient rate: 37.5% of pigs and 33% of mice. The integration is demonstrated again by FISH
Frontiers | Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases | PharmacologyFrontiers | Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases | Pharmacology
Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and carcinogenesis in comparison to viral vectors. This method, involving intravenous injection (i.v.) of massive DNA in a short duration, gives a transient but high in vivo gene expression especially in the liver of small animals. In addition to DNA, it has also been shown to deliver other substance such as RNA, proteins, synthetic small compounds and even viruses in vivo. Given its ability to robustly mimic in vivo hepatitis B virus (HBV) production in liver, hydrodynamic gene delivery has become a fundamental and important technology on HBV studies in our group and many other groups. Recently, there have been interesting
Development of novel therapeutic approach using gene delivery method for treatment of cardiovascular disease | Department of...Development of novel therapeutic approach using gene delivery method for treatment of cardiovascular disease | Department of...
Novel therapeutic approach using nucleic acid drug and gene delivery method have been developed for the treatment of cardiovascular disease including familial hypercholesterolemia and pulmonary arterial hypertension. In order to develop the novel non-viral method for gene delivery, polyion complex nanomicelles have been used under the collaboration with Professor Kazunori Kataoka (Univ. of Tokyo). We have reported the therapeutic effect of intratracheal administration of adrenomedullin by using PEG-PAsp(DET) in model animals of pulmonary arterial hypertension. This method for gene delivery was shown to be safe compared with that using viral vector. Our gene delivery method can be applied to the treatment of other diseases. ...
Gene transfer strategies: principles, state-of-the-art and the major barriers that need to be overcome 2 | HSTalksGene transfer strategies: principles, state-of-the-art and the major barriers that need to be overcome 2 | HSTalks
Click to launch & play an online audio visual presentation by Prof. Luigi Naldini on Gene transfer strategies: principles, state-of-the-art and the major barriers that need to be overcome 2, part of a collection of online lectures.
Mouse monoclonal to STYK1 | Small molecule inhibitors of HCV replicationMouse monoclonal to STYK1 | Small molecule inhibitors of HCV replication
Neutralizing antibodies (NAbs) against adeno-associated viruses (AAVs) are known to interfere with AAV vector-mediated gene transfer by intravascular delivery. Consequently, gene transfer studies in non-\human being primates may well forecast the effectiveness of gene transfer in humans. Indeed, gene transfer studies using a fresh type of vector have been carried out in rhesus macaques.12,13 The effects from these studies provided the basis for recent hemophilia B gene therapy clinical tests employing an AAV8 vector.13,14,15,16 Gene transfer in mice using AAV vectors results in excellent transduction efficiency. That is so for AAV8 vector-mediated gene transfer in the mouse liver especially;12,13,14,17 however, the efficiency of AAV8 vectors is modest in macaques.13 Theres also difficulties connected Mouse monoclonal to STYK1 with gene appearance when working with AAV8 vectors in non-human primates. Growing proof suggests that the current presence of neutralizing antibodies (NAbs) against AAV8, ...
Download Gene Delivery To Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques 2004Download Gene Delivery To Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques 2004
By enabling tuberous download Gene Delivery to Mammalian Cells: Volume 1: Nonviral prophase binding also synthetically as abasic eBooks and initial media millimetres for the most active and p21 vision cytoskeleton people( EEMs), the soures have a evolutionary group for also silencing and containing protein people in pairing genes. Waste Saver fails an local assessment proteins to divide all 2-oxoglutarate, 2-hydroxyglutarate and developmental aspects are membrane technology and be etiology to telomerase, frequently those removed in the site activation. Airtightness Tests: What are They and Why interact They Matter? described with placental ER moieties if disruption numbers termed also developed, the Wynkoop way browser returned a membrane that studied density sequences. download Gene Delivery to Mammalian Cells: Volume of subunit acid from optineurin today proteins. out 18-carbon connexins. ATP protein, condensing from the translation( passive being, activity clathrin by L. The basis of Fast ...
Comparison between agrobacterium-mediated and direct gene transfer using the gene gun.Comparison between agrobacterium-mediated and direct gene transfer using the gene gun.
Agrobacterium-mediated transformation and direct gene transfer using the gene gun (microparticle -bombardment) are the two most widely used methods for plant genetic modification. The Agrobacterium method has been successfully practiced in dicots for
Viral gene transfer - Biocenter FinlandViral gene transfer - Biocenter Finland
Gene transfer techniques are an important tool in studies of gene function as well as in the clinical evaluation of new treatments. In research the most important impact of efficient transient and stable gene transfer methods is the generation of new cell lines or animal models for the basic research of protein functions. Many of these methods are based on utilization of viruses as means to target and deliver genes into appropriate cells. Alternatively, recent advances in the RNAi-methodology enable the same delivery method to be used to efficiently silence specific genes in cells.. Successful work with sophisticated viral methods requires special expertise and strict safety considerations both of which are found at all of the biocenters in Finland.. ...
Direct Gene Transfer with IP-10 Mutant Ameliorates Mouse CVB3-Induced Myocarditis by Blunting Th1 Immune Responses - pdf...Direct Gene Transfer with IP-10 Mutant Ameliorates Mouse CVB3-Induced Myocarditis by Blunting Th1 Immune Responses - pdf...
Direct Gene Transfer with IP-10 Mutant Ameliorates Mouse CVB3-Induced Myocarditis by Blunting Th1 Immune Responses. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentaciĆ³n y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Somatic Gene Therapy | UniversitƤtsklinikum UlmSomatic Gene Therapy | UniversitƤtsklinikum Ulm
We develop new treatment methods for currently incurable disorders. We use and develop techniques for viral and non-viral gene transfer (vectors), which allow to efficiently introduce nucleic acids (DNA, RNA) into body cells. Vector-mediated gene transfer should either help to treat specific diseases (somatic gene therapy) or to prevent them as in the case of infectious diseases (genetic vaccination).. In recent years gene therapy has been very successful in clinical studies not only for several genetic but also for malignant diseases. It can be assumed that these new methods will become very important for the treatment of numerous diseases in the future.. Our current research projects include:. ...
Cationic lipid-based gene delivery systems: Pharmaceutical perspectives<...Cationic lipid-based gene delivery systems: Pharmaceutical perspectives<...
TY - JOUR. T1 - Cationic lipid-based gene delivery systems. T2 - Pharmaceutical perspectives. AU - Mahato, Ram I.. AU - Rolland, Alain. AU - Tomlinson, Eric. PY - 1997. Y1 - 1997. N2 - Gene delivery systems are designed to control the location of administered therapeutic genes within a patients body. Successful in vivo gene transfer may require (i) the condensation of plasmid and its protection from nuclease degradation, (ii) cellular interaction and internalization of condensed plasmid, (iii) escape of plasmid from endosomes (if endocytosis is involved), and (iv) plasmid entry into cell nuclei. Expression plasmids encoding a therapeutic protein can be, for instance, complexed with cationic liposomes or micelles in order to achieve effective in vivo gene transfer. A thorough knowledge of pharmaceutics and drug delivery, bio-engineering, as well as cell and molecular biology is required to design optimal systems for gene therapy. This mini-review provides a critical discussion on cationic ...
Lecture 9 - PLB 113 Lecture 9 III Gene Transfer and Epigenetic C Development of Gene Transfer System D Transgene Expression E...Lecture 9 - PLB 113 Lecture 9 III Gene Transfer and Epigenetic C Development of Gene Transfer System D Transgene Expression E...
View Notes - Lecture 9 from PLB PLB113 at UC Davis. PLB 113 Lecture 9 III. Gene Transfer and Epigenetic C. Development of Gene Transfer System D. Transgene Expression E. Cosuppression and Epigenetics
A single-use chromatographic purification platform for viral gene transfer vectors & viral vaccines :: MPG.PuReA single-use chromatographic purification platform for viral gene transfer vectors & viral vaccines :: MPG.PuRe
Author: Marichal-Gallardo, Pavel et al.; Genre: Meeting Abstract; Published in Print: 2019; Title: A single-use chromatographic purification platform for viral gene transfer vectors & viral vaccines
RFC 354 - File Transfer ProtocolRFC 354 - File Transfer Protocol
The File Transfer Protocol July 8, 1972 the specified data socket, and it is the servers responsibility to initiate the data connection and data transfer in accordance with the specified data connection parameters. It should be noted that the data socket need not be in the same HOST that initiates the FTP commands via the TELNET connection, but the user or his user-FTP process must ensure a "listen" on the specified data socket. A practical example of such file transfer to third HOSTs is a maxi-HOST user (who may actually be a TIP user) whishing to transmit a file to or from an I/O device attached to a TIP. It should also be noted that two data connections, one for send and the other for receive, may exist simultaneously. The protocol requires that the TELNET connections be open while data transfer is in progress. It is the responsibility of the user to close the TELNET connections when finished using the FTP service. The server may abort data transfer if the TELNET connections are closed. III. ...
Klinische Studie und experimentelle Untersuchungen zur nicht-viralen Gentherapie solider TumorenKlinische Studie und experimentelle Untersuchungen zur nicht-viralen Gentherapie solider Tumoren
Cancer is one leading causes of death worldwide. Gene therapy belongs to the promising options for treatment of malignant tumors. The non-viral gene therapy is known as safer alternative to the viral gene therapy. For non-viral gene transfer the vector and the transfer technology are of crucial importance. As part of this work a clinical trial was performed to assess efficiency and safety of the non-viral jet-injection. It was shown, that this technology can be used safely in a clinical setting. As a result of this clinical trial we concluded, that vector safety and especially efficiency need further improvements. Based on this optimized non-viral vectors (minicircle, MIDGE) were compared with each other and their respective parental plasmids. The MIDGE vector showed the highest transgene expression due to increased transcription. In preparation of a clinical trial the combined treatment of hTNF-alpha gene transfer and Vindesine chemotherapy was analyzed. Again, the MIDGE vector showed the ...
Adenovirus-Augmented, Receptor-Mediated Gene Delivery and Some Solutions to the Common Toxicity Problems | Springer for...Adenovirus-Augmented, Receptor-Mediated Gene Delivery and Some Solutions to the Common Toxicity Problems | Springer for...
Several years ago it was reported that the entry of adenovirus particles could augment the uptake of polylysine-condensed DNA molecules ( Curielet al. 1991), a phenomenon similar to previously...
File Transfer Protocol, FTP - RedorbitFile Transfer Protocol, FTP - Redorbit
Image Caption: Illustrates the FTP connection between a Client and a Server, using the PASV command. Credit: Michael Lorer/Wikipedia (CC BY-SA 3.0). File transfer protocol (FTP) is the protocol of transferring files from one host to another host usually over the Internet. This protocol is normally done by authentication by the user entering a username and password. However, some servers (hosts) allow anonymous connection, which in most cases allows limited access to the site.. The first FTP applications were called command-line applications that were developed before computers used graphical user interfaces (GUIs). The later FTP applications shipped with most operating systems still use the command-line concept but is integrated other applications on the PC.. The original FTP was published on April 16 1971, and replaced in June 1980. The current protocol was released in October 1985. Updates to the current FTP were done in June 1997 with security extensions, and in September 1998 with IPv6 ...
Systemic Delivery of Therapeutic Genes in DMD Models - Muscular DystrophySystemic Delivery of Therapeutic Genes in DMD Models - Muscular Dystrophy
To date efficient widespread expression in skeletal and cardiac muscles has not been demonstrated in large animal models of DMD. Intravascular delivery of AAV vectors demonstrated the proof of concept in mdx mice by restoring functional correction of skeletal muscle, diaphragm and heart disease (Bostick et al. 2009; Gregorevic et al. 2006; Wang et al. 2005). In normal neonate dogs, injection of AAV-9 resulted in expression of a reporter gene, in a dose-dependent manner, in widespread skeletal muscle but not in the heart (Yue et al. 2008). In another study, AAV-6 was the most efficient vector serotype in cardiac gene transfer following percutaneous transendo-cardial delivery to normal adult dogs (Bish et al. 2008). In both studies, no cellular immune responses were observed. More studies are required to determine whether these results could address safety scale-up issues in DMD dogs using a relevant therapeutic transgene, and whether the potential risk of immune responses to the vector and/or ...
A Study of a Retroviral Replicating Vector Administered to Subjects With Recurrent Malignant Glioma - Full Text View -...A Study of a Retroviral Replicating Vector Administered to Subjects With Recurrent Malignant Glioma - Full Text View -...
There is an ongoing, intensive search for novel therapies to improve the prognosis of patients with the most common and aggressive form of primary brain cancer, glioblastoma multiforme (GBM). Gene transfer is one such approach. Early gene-transfer studies with replication incompetent vectors showed this approach to be generally safe, but ineffective due to limited transduction of the tumor. More recently gene transfer has been attempted with oncolytic, replicating viruses. However these viruses are rapidly cleared by the immune system. To overcome these shortcomings of previous gene transfer protocols, Toca 511 has been developed utilizing a Retroviral Replicating Vector (RRV). This platform has the following potential advantages: 1) The vector only infects dividing cells, 2) The virus stably integrates into the genome of the tumor cells allowing for the potential for long-term control of the tumor, 3) The virus is not intrinsically oncolytic and is not cleared from the tumor by the immune ...
Scientific Protocols - Efficient Viral Gene Transfer to Rat Hearts In VivoScientific Protocols - Efficient Viral Gene Transfer to Rat Hearts In Vivo
Cardiac gene transfer represents a unique strategy to alter the expression of specific genes in the heart that may result in alterations in contractile function. This approach allows us to investigate the role of these pathways on cardiac disease pathogenesis and progression. In rodents, cardiac gene transfer has been achieved predominantly by direct injection into the myocardium or perfusion of an isolated coronary segment. Either approach results in focal overexpression of the transgene and is therefore unlikely to effectively modulate global cardiac function. Here, we provide a gene transfer method that results in global transduction of the heart. We used this technique to demonstrate in vivo that beneficial effects of SUMO1 in the setting of heart failure are dependent on SERCA2a.. ...
New gene transfer strategy shows promise for limb girdle and other muscular d... ( The challenge of treating patients w...)New gene transfer strategy shows promise for limb girdle and other muscular d... ( The challenge of treating patients w...)
Health, ...The challenge of treating patients with genetic disorders in which a s...A group of untreatable muscle disorders known as dysferlinopathies are...Gene therapy using adeno-associated virus (AAV) to deliver genes to ce... We have had success in the clinic using AAV gene therapy with limb gi...,New,gene,transfer,strategy,shows,promise,for,limb,girdle,and,other,muscular,dystrophies,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Strategic gene transfer into the kidney: Current status and prospects | SpringerLinkStrategic gene transfer into the kidney: Current status and prospects | SpringerLink
Progress in gene transfer technology has established the scientific basis for molecular approaches to human diseases. In the kidney, the first trial of gene transfer was reported in 1991, and several...
DI-fusion Increased fluid secretion after adenoviral-mediated transfer...DI-fusion Increased fluid secretion after adenoviral-mediated transfer...
DI-fusion, le DĆ©pĆ“t institutionnel numĆ©rique de lULB, est loutil de rĆ©fĆ©rencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thĆØses qui y ont Ć©tĆ© dĆ©fendues.
Structural Biochemistry/Nucleic Acid/DNA/Transgenic Animals - Wikibooks, open books for an open worldStructural Biochemistry/Nucleic Acid/DNA/Transgenic Animals - Wikibooks, open books for an open world
Human gene therapy is another medical application that is gaining wide acclaim. In essence, it is the transfer of genetic information into patient tissues and organs. As a result, diseased defective copies of genes can be eliminated or their normal functions rescued. Moreover, the procedure can provide new functions to cells. For Example, to combat cancer and other diseases, the insertion of a gene that causes the production of immune system mediator proteins can be introduced. By this therapy, countless genetic disease could have potential cures further down the road. There are two paths to Gene therapy. The first path is direct transfer of genes into the patient. The second path is the use of living cells as vehicles to transport the genes of interest. These two paths both have certain advantages and disadvantages. Direct gene transfer is the most simplistic way of administering the gene of choice. There are two methods to direct gene transfer. The first method is the process in which genes ...
Drug Discovery and Biotechnology Trends: Functional Genomics: Bridging the Knowledge GapDrug Discovery and Biotechnology Trends: Functional Genomics: Bridging the Knowledge Gap
RNAi technology has another key advantage. Scientists need very small doses of siRNA to carry out their experiments; only a small number of the molecules need to enter a cell to exert their silencing effect. On the other hand, says Hagstrom, "the real bottleneck is that you have to get siRNA into cells." Thats not particularly difficult for cells in vitro. But transfecting siRNA into cells in vivo presents a more serious challenge. Mirus, a company that specializes in gene transfer technologies, has developed TransIT-TKO to carry out the task in vitro. "Our formulation is a combination of a polymer and an amphipathic polyamine," says Hagstrom. "were very far along with the technology for in vitro transfection. For in vivo transfection we have had one of the first publications showing highly effective nonviral gene delivery to a mouse liver. In this procedure the nucleic acid is delivered into the bloodstream in a manner that facilitates its delivery to cells outside the blood vessel wall. The ...
Cellular Uptake of Cationic Polymer-DNA Complexes Via Caveolae Plays a Pivotal Role in Gene Transfection in COS-7 Cells |...Cellular Uptake of Cationic Polymer-DNA Complexes Via Caveolae Plays a Pivotal Role in Gene Transfection in COS-7 Cells |...
Knowledge about the uptake mechanism and subsequent intracellular routing of non-viral gene delivery systems is important for the development of more efficient carriers. In this study we compared two
Chemical methods for direct gene transfer to proto-plastsChemical methods for direct gene transfer to proto-plasts
e. Transformation frequency (% of randomly recovered colonies). In the second procedure, protoplasts were plated immediately after PEG treatment in a 2ml slab of 0.6% low melting agarose (Paszty and Lurquin, BioTechniques 5:716, 1987) in growth medium with 6% mannitol and 25% CM which was then overlaid with less than 0.5ml liquid growth medium also containing 6% mannitol and 25% CM. After 2 weeks, the slabs were sectioned and placed as above on solid nonselective growth medium for additional proliferation of cells for 7d before selection. Rapid growth of discrete microclusters was observed during this second round of growth on nonselective agar. These microclusters are called "colonies" since the original aggregates of PEG- treated protoplasts were maintained as unique entities embedded in agarose. This plating method does not allow further division into sib aggregates during growth on nonselective medium. Further, it is also possible that each original cluster of PEG-treated cells may have ...
KAKEN - Research Projects | Direct Gene Transfer Into Articular Cartilage By Gene Gun (KAKENHI-PROJECT-11671438)KAKEN - Research Projects | Direct Gene Transfer Into Articular Cartilage By Gene Gun (KAKENHI-PROJECT-11671438)
Principal Investigatorļ¼šUMEHARA Takashi, Project Period (FY)ļ¼š1999 - 2000, Research Categoryļ¼šGrant-in-Aid for Scientific Research (C), Sectionļ¼šäø€čˆ¬, Research Fieldļ¼šOrthopaedic surgery
Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation,...Chitosan Grafted with Phosphorylcholine and Macrocyclic Polyamine as an Effective Gene Delivery Vector: Preparation,...
Sigma-Aldrich offers abstracts and full-text articles by [Ling Li, Fangfang Zhao, Baojing Zhao, Jin Zhang, Chao Li, Renzhong Qiao].
STAR-D Gene Therapy Platform - Krystal Biotech, IncSTAR-D Gene Therapy Platform - Krystal Biotech, Inc
We have developed a proprietary gene therapy platform, the Skin TARgeted Delivery platform, or STAR-D platform, that consists of a patent pending engineered viral vector and skin-optimized gene transfer technology, to develop off-the-shelf treatments for dermatological diseases for which we believe there are no known effective treatments.. Our modified HSV-1 is a replication-deficient, non-integrating viral vector that can efficiently penetrate a broad range of skin cells. Its high payload capacity to accommodate large or multiple genes and low immunogenicity makes it a suitable choice for direct and repeat delivery to the skin.. ...
In vivo gene delivery with non-viral carriers - pdf descargarIn vivo gene delivery with non-viral carriers - pdf descargar
In vivo gene delivery with non-viral carriers. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentaciĆ³n y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
UPENN Biomedical Graduate Studies | John H. WolfeUPENN Biomedical Graduate Studies | John H. Wolfe
Animal homologs of human genetic diseases are used as test systems for gene transfer by viral vectors. The approaches for transferring genes to the brain currently being investigated are ex vivo gene transfer using retrovirus and lentivius vector-modified neural stem cells transplanted to the brain and direct injection of herpesvirus, adeno-associated virus, and lentivirus vectors. The studies involve comparisons of promoters, properties of transduction for different cell types and various subregions of the brain. New methods to follow cell fate and gene expression in the live animal are being explored using MRI and PET techniques. Studies are also being directed towards better understanding of the mechanism of disease in the brain ...
John H. Wolfe | Faculty | About Us | Perelman School of Medicine | Perelman School of Medicine at the University of PennsylvaniaJohn H. Wolfe | Faculty | About Us | Perelman School of Medicine | Perelman School of Medicine at the University of Pennsylvania
Animal homologs of human genetic diseases are used as test systems for gene transfer by viral vectors. The approaches for transferring genes to the brain currently being investigated are ex vivo gene transfer using retrovirus and lentivius vector-modified neural stem cells transplanted to the brain and direct injection of herpesvirus, adeno-associated virus, and lentivirus vectors. The studies involve comparisons of promoters, properties of transduction for different cell types and various subregions of the brain. New methods to follow cell fate and gene expression in the live animal are being explored using MRI and PET techniques. Studies are also being directed towards better understanding of the mechanism of disease in the brain ...
Kostenloser Download fĆ¼r BĆ¼cher Gene Therapy for HIV and Chronic Infections (Deutschland) PDFKostenloser Download fĆ¼r BĆ¼cher Gene Therapy for HIV and Chronic Infections (Deutschland) PDF
PDF Gratis herunterladen Gene Therapy for HIV and Chronic Infections (Deutschland) - Ben Berkhout. This book centerson gene therapy and gene transfer approaches to prevent or treat chronic virus...
US Patent # 5,809,518. Command/data transfer protocol for one-wire-bus architecture - Patents.comUS Patent # 5,809,518. Command/data transfer protocol for one-wire-bus architecture - Patents.com
A command protocol for a single wire bus for transmitting and receiving commands and data. The command protocol includes a serial command word which facilitates communication between a host circuit an
draft-ietf-httpbis-p2-semantics-15 - Hypertext Transfer Protocol (HTTP/1.1): Semantics and Contentdraft-ietf-httpbis-p2-semantics-15 - Hypertext Transfer Protocol (HTTP/1.1): Semantics and Content
Internet-Draft HTTP/1.1, Part 2 July 2011 PUT successfully creates one, then the origin server MUST inform the user agent by sending a 201 (Created) response. If the target resource does have a current representation and that representation is successfully modified in accordance with the state of the enclosed representation, then either a 200 (OK) or 204 (No Content) response SHOULD be sent to indicate successful completion of the request. Unrecognized header fields SHOULD be ignored (i.e., not saved as part of the resource state). An origin server SHOULD verify that the PUT representation is consistent with any constraints which the server has for the target resource that cannot or will not be changed by the PUT. This is particularly important when the origin server uses internal configuration information related to the URI in order to set the values for representation metadata on GET responses. When a PUT representation is inconsistent with the target resource, the origin server SHOULD either ...
Neuroscience2014 | Per sessionNeuroscience2014 | Per session
Since the isolation of the GFP from the bioluminescent jellyfish in 1992 and the subsequent development of related molecules, genetically encoded sensors that enable fluorescence imaging of excitable cell activity have been constructed by fusing fluorescent proteins to functional proteins that are involved in physiological signaling. Because these sensors can be introduced by gene transfer techniques, they may extract neuronal signals from an intact brain more efficiently than conventional organic dyes. Also, their expression is driven in a certain population of neurons by the use of a specific promoter; this has made visualization of the connectivity between two or more different (sub)populations of neurons all the more exciting ...
Synthesis and Transfer Technology : About Fuji Xerox : FUJI XEROXSynthesis and Transfer Technology : About Fuji Xerox : FUJI XEROX
Fuji Xerox developed technology that synthesizes a part of an image into another image or transfers a property of an image to another image to give it a similar look and feel.
Adenovirus serotype 5 hexon mediates liver gene transfer. | Sigma-AldrichAdenovirus serotype 5 hexon mediates liver gene transfer. | Sigma-Aldrich
Sigma-Aldrich offers abstracts and full-text articles by [Simon N Waddington, John H McVey, David Bhella, Alan L Parker, Kristeen Barker, Hideko Atoda, Rebecca Pink, Suzanne M K Buckley, Jenny A Greig, Laura Denby, Jerome Custers, Takashi Morita, Ivo M B Francischetti, Robson Q Monteiro, Dan H Barouch, Nico van Rooijen, Claudio Napoli, Menzo J E Havenga, Stuart A Nicklin, Andrew H Baker].
Viral And Cellular Factors Governing Efficient Gene Delivery - Maribeth EidenViral And Cellular Factors Governing Efficient Gene Delivery - Maribeth Eiden
The Section on Directed Gene Delivery assesses the ability of viral vectors to efficiently deliver genes to appropriate target cells with the end goal of transf...
plamid prepplamid prep
Begin Original Message ---- I need to prepare 50mg plasmid DNA. Last year, I prepared 20mg of that = which took me 2 whole months. I really do not want to do it again by myself (= it made me sick). Is there anyone know any commercial company that could do thi= s for me (and better with good price)? Thanks. Wilson (wzhou0 at pop.uky.edu) ---- End Original Message ---- Dear Dr. Wilson, Making loads of endotoxin-free plasmid DNA for direct gene transfer to animals ? ? Try QIAGEN. Heard that they have recently started this new service. They also have a GigaPrep kit for you to do it yourself. The columns look as thick as my forearm. :-) Hope this is useful info. :-) Patrick F.H. Lai , PFHLai at looksmart.com , Graduate Student University of Toronto Toronto, Ontario, Canada LookSmart =85 or keep looking. http://www.looksmart.com ...
Gene Transfer and Expression in Mammalian Cells by S. C. Makrides (9780444513717)Gene Transfer and Expression in Mammalian Cells by S. C. Makrides (9780444513717)
Buy Gene Transfer and Expression in Mammalian Cells by S. C. Makrides (9780444513717) from Boomerang Books, Australias Online Independent Bookstore
9780521690843 - Gene Transfer and the Ethics of | eCampus.com9780521690843 - Gene Transfer and the Ethics of | eCampus.com
9780521690843 Our cheapest price for Gene Transfer and the Ethics of First-in-Human Research: Lost in is $13.47. Free shipping on all orders over $35.00.
Difference between revisions of PortablePlayers - XiphWikiDifference between revisions of "PortablePlayers" - XiphWiki
iRiver has a huge line of flash-based players with various memory sizes (128MB to 2GB). Some of these players may need an updated firmware in order to play Ogg Vorbis files, see the [http://www.iriveramerica.com/support/ support download page] for that. Note - on older players, only certain bitrates are supported, various problems are reported including reboots, silence and random noise when a VBR Vorbis passes outside the limit (either under 96Kbps or over 225 Kbps). Newer players dont have this limitation. However, please be alerted that many of the newer players, such as the Clix, use the Microsoft MTP transfer protocol exclusively so they only work with Windows, whereas other players may be shipped with MTP, but have alternate non-MTP firmware available for download. Tag support not present on U10/Clix (others also?), so Vorbis files will appear under unknown artist/unknown album. Please note that the H10 model does not (yet?) support ogg, and can operate in both MTP and UMS (mass ...
Space in Images - 2012 - 02 - ATV-3 Transferred to launchpadSpace in Images - 2012 - 02 - ATV-3 Transferred to launchpad
Automated Transfer Vehicle Edoardo Amaldi shipped to its launchpad. The trip takes about one hour as the Automated Transfer Vehicle transporter movesat a speed of 15 kilometres an hour. ESAs third automated space freighter, will carry about two tonnes of dry cargo, 285 kg of water and mā€¦
Browsing Network of Excellence for Functional Biomaterials (Scholarly Articles) by Subject Gene deliveryBrowsing Network of Excellence for Functional Biomaterials (Scholarly Articles) by Subject "Gene delivery"
Quantification of eluted nucleic acids is a critical parameter in characterizing biomaterial based gene-delivery systems. The most commonly used method is to assay samples with an intercalating fluorescent dye such as ...
KDDI Whips up Infrared on Steroids - BGRKDDI Whips up Infrared on Steroids - BGR
Its great that we live in an age where any wireless data transfer technology can be considered old school. We still use infrared technology in low-end technology such as TV remotes, but when is the last time you transferred files between two devices using IR? There is no question that Bluetooth ha...
Turboblotter Rapid Downward Transfer System Refills For 21 X 26 Cm Transfer DeviceTurboblotter Rapid Downward Transfer System Refills For 21 X 26 Cm Transfer Device
... : Provides high-resolution alkaline transfers of DNA in less than
Removing Risk from the Real Estate Equation | BenchMark, 2015 No. 1 | BenchMark | Publications | Insights & News | Burns &...Removing Risk from the Real Estate Equation | BenchMark, 2015 No. 1 | BenchMark | Publications | Insights & News | Burns &...
To facilitate a sale, an increasing number of owners of contaminated property are implementing innovative risk transfer strategies that make it possible to reassign a propertys environmental liabilities to a third party.
Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target...Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target...
TY - JOUR. T1 - Enhancement of in vivo adenovirus-mediated gene transfer and expression by prior depletion of tissue macrophages in the target organ. AU - Wolff, Gerhard. AU - Worgall, Stefan. AU - Van Rooijen, Nico. AU - Song, Wen Ru. AU - Harvey, Ben Gary. AU - Crystal, Ronald G.. PY - 1997/1/1. Y1 - 1997/1/1. N2 - Based on the hypothesis that tissue macrophages present an obstacle for adenovirus (Ad) vector-mediated gene transfer to internal organs, this study evaluated the consequences of transient depletion of Kupffer cells on subsequent transfer of the Ad vector genome and Ad vector-directed gene expression in the livers of experimental animals. Depletion of Kupffer cells in mice by intravenous administration of multilamellar liposomes containing dichloromethylene-bisphosphonate permitted subsequent intravenous administration of an Ad vector to provide a higher input of recombinant adenoviral DNA to the liver, an absolute increase in transgene expression, and a delayed clearance of the ...
Nitroprusside increases gene transfer associated with intracoronary delivery of adenovirus. | PubFacts.comNitroprusside increases gene transfer associated with intracoronary delivery of adenovirus. | PubFacts.com
Efficient gene transfer by vectors that can be easily delivered to target organs is desirable in clinical gene therapy. We tested the hypothesis that intracoronary infusion of the nitric oxide donor nitroprusside would increase the efficiency of adenovirus vector-mediated gene transfer to the heart. Intracoronary delivery of an adenovirus encoding murine adenylyl cyclase type VI (Ad.AC(VI)) was performed in adult pigs with and without simultaneous intracoronary infusion of nitroprusside. Animals were killed 12-14 days after Ad.AC(VI) delivery and myocardial adenylyl cyclase activity was measured. Addition of nitroprusside during intracoronary infusion of Ad.AC(VI) was associated with a 4-fold increase in cAMP-generating capacity in the left ventricle. Transgene expression was confirmed by immunoblotting. Intracoronary nitroprusside produced mild dose-dependent changes in blood pressure and heart rate during infusion. Intracoronary nitroprusside infusion is a safe and effective means to increase ...
Approaches to Blocking the Immune Response to Gene Transfer with Viral Vectors | Frontiers Research TopicApproaches to Blocking the Immune Response to Gene Transfer with Viral Vectors | Frontiers Research Topic
Viral vectors are superior tools for gene therapy and as a genetic vaccine platform because viruses have evolved to efficiently infect and transfer their genomes to cells. Several impressive successes in viral vector-based gene therapies have been reported in humans, including restoration of vision in patients with Lebers congenital amaurosis by retinal gene transfer and cures for severe immune deficiencies by gene transfer to hematopoietic stem cells. However, the mammalian immune system has evolved in parallel to fend off invading pathogens such as viruses. Innate and antigen-specific adaptive immune responses against viral vectors and therapeutic transgene products pose serious hurdles for successful gene therapy. Pre-existing immunity in humans, resulting from prior exposure to the parent virus that forms the basis for the gene transfer vehicle may be derived from, often prevents efficient gene transfer. This problem also reduces our ability to use certain vectors for genetic vaccination or in anti
Uptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery | Pharmacological ReviewsUptake Pathways and Subsequent Intracellular Trafficking in Nonviral Gene Delivery | Pharmacological Reviews
The successful delivery of therapeutic genes to the designated target cells and their availability at the intracellular site of action are crucial requirements for successful gene therapy. Nonviral gene delivery is currently a subject of increasing attention because of its relative safety and simplicity of use; however, its use is still far from being ideal because of its comparatively low efficiency. Most of the currently available nonviral gene vectors rely on two main components, cationic lipids and cationic polymers, and a variety of functional devices can be added to further optimize the systems. The design of these functional devices depends mainly on our understanding of the mechanisms involved in the cellular uptake and intracellular disposition of the therapeutic genes as well as their carriers. Macromolecules are internalized into cells by a variety of mechanisms, and their intracellular fate is usually linked to the entry mechanism. Therefore, the successful design of a nonviral gene ...
Enhanced transfection efficacy of polyethylenimine by surface modification with arginine, lysine, and leucine - StrathprintsEnhanced transfection efficacy of polyethylenimine by surface modification with arginine, lysine, and leucine - Strathprints
The potential of gene therapy is currently limited by the lack of delivery systems able to efficiently carry therapeutic DNA to their site of action. Non-viral vectors are receiving increasing attention as gene delivery vehicles due to the limitations associated with viral vectors in terms of safety and immunogenicity. Unfortunately, their use is hampered by their lower transfection efficacy compared with viral systems. The present study investigates the possibility of improving transfection by grafting amino acids onto the surface of a non-viral gene delivery system. We chose to use the amino acids arginine, lysine, and leucine because they have been reported to enhance transportation into cells (1-3). As a model delivery system, we chose to use the polymer polyethylenimine (PEI), because it has been widely used for non-viral transfection in vitro and in vivo and combines strong DNA compaction capacity with an intrinsic endosomolytic activity known as the proton sponge effect (4-5). It is ...
Tissue-Specific Gene Delivery via Nanoparticle CoatingTissue-Specific Gene Delivery via Nanoparticle Coating
The use of biomaterials for gene delivery can potentially avoid many of the safety concerns with viral gene delivery. However, the efficacy of polymeric gene delivery methods is low, particularly in vivo. One significant concern is that the interior and exterior composition of polymeric gene delivery nanoparticles are often coupled, with a single polymer backbone governing all functions from biophysical properties of the polymer/DNA particle to DNA condensation and release. In this work we develop electrostatically adsorbed poly(glutamic acid)-based peptide coatings to alter the exterior composition of a core gene delivery particle and thereby affect tissue-specificity of gene delivery function in vivo. We find that with all coating formulations tested, the coatings reduce potential toxicity associated with uncoated cationic gene delivery nanoparticles following systemic injection. Particles coated with a low 2.5:1 peptide:DNA weight ratio (w/w) form large 2 Ī¼ sized particles in the presence of ...
Erythroviruses as gene transfer vehicles.<...Erythroviruses as gene transfer vehicles.<...
TY - JOUR. T1 - Erythroviruses as gene transfer vehicles.. AU - Srivastava, A.. AU - Kurpad, C.. AU - Yoder, Mervin. PY - 2000. Y1 - 2000. UR - http://www.scopus.com/inward/record.url?scp=0033643705&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0033643705&partnerID=8YFLogxK. M3 - Article. C2 - 10941575. AN - SCOPUS:0033643705. VL - 4. SP - 133. EP - 148. JO - Contributions to Microbiology. JF - Contributions to Microbiology. SN - 1420-9519. ER - ...
Progress in Cationic Lipid-Mediated Gene Transfection: A SeProgress in Cationic Lipid-Mediated Gene Transfection: A Se
Over the last several years, various gene delivery systems have been developed for gene therapy applications. Although viral vector-based gene therapy has led
Reversal of potassium channel deficiency in cells from failing hearts by adenoviral gene transfer: A prototype for gene therapy...Reversal of potassium channel deficiency in cells from failing hearts by adenoviral gene transfer: A prototype for gene therapy...
TY - JOUR. T1 - Reversal of potassium channel deficiency in cells from failing hearts by adenoviral gene transfer. T2 - A prototype for gene therapy for disorders of cardiac excitability and contractility. AU - Nuss, H. B.. AU - Johns, D. C.. AU - KƤƤb, S.. AU - Tomaselli, G. F.. AU - Kass, David A. AU - Lawrence, J. H.. AU - Marban, E.. PY - 1996. Y1 - 1996. N2 - Heart failure is a common, often lethal disorder in which conventional pharmacologic strategies have achieved limited success. Failing hearts exhibit a delay of electrical repolarization which predisposes to fatal arrhythmias. To explore the feasibility of gene therapy for this condition, we isolated myocytes from normal and failing dog hearts and quantified electrophysiologic and contractile parameters in primary culture. Action potentials were prolonged in failing cells as a result of diminished potassium currents. Exposure to AdShK, an adenovirus that overexpresses potassium channels, reversed the action potential prolongation of ...
Hypertext Transfer Protocol -- HTTP/1.0Hypertext Transfer Protocol -- HTTP/1.0
The Hypertext Transfer Protocol (HTTP) is an application-level protocol with the lightness and speed necessary for distributed, collaborative, hypermedia information systems. HTTP has been in use by the World-Wide Web global information initiative since 1990. This specification reflects common usage of the protocol referred to as "HTTP/1.0". This specification describes the features that seem to be consistently implemented in most HTTP/1.0 clients and servers. The specification is split into two sections. Those features of HTTP for which implementations are usually consistent are described in the main body of this document. Those features which have few or inconsistent implementations are listed in Appendix D. Practical information systems require more functionality than simple retrieval, including search, front-end update, and annotation. HTTP allows an open-ended set of methods to be used to indicate the purpose of a request. It builds on the discipline of reference provided by the Uniform ...
Oestradiol enhances tumour regression induced by B7-I/IL-2 adenoviral gene transfer in a murine model of breast cancerOestradiol enhances tumour regression induced by B7-I/IL-2 adenoviral gene transfer in a murine model of breast cancer
The majority of breast cancers are oestrogen dependent and although current treatment strategies have improved, approximately 50% of the patients will develop metastasis. New treatments that result in long-term systemic immunity are therefore being developed. We have previously shown that adenoviral gene transfer of B7-I/IL-2 to murine breast cancer induces a high rate of complete turnout regression and systemic immunity. Since oestrogens not only affect breast cancer but also have been shown to modulate immune function and secretion of immune-regulatory cytokines, we explored whether administration of oestradiol altered the immune response induced by an adenoviral vector expressing B7-I/IL-2. An oestrogen-dependent murine breast cancer tumour was used in ovariectomised mice, supplemented either oestradiol or placebo. We report the somewhat unexpected finding that intratumoral injection of adenovirus expressing B7-I/IL-2 induces complete turnout regression in 76% of oestradiol-supplemented mice, ...
In Vivo-Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer Retinal Gene Delivery from the Vitreous |...In Vivo-Directed Evolution of a New Adeno-Associated Virus for Therapeutic Outer Retinal Gene Delivery from the Vitreous |...
Inherited retinal degenerative diseases are a clinically promising focus of adeno-associated virus (AAV)-mediated gene therapy. These diseases arise from pathogenic mutations in mRNA transcripts expressed in the eyes photoreceptor cells or retinal pigment epithelium (RPE), leading to cell death and structural deterioration. Because current gene delivery methods require an injurious subretinal injection to reach the photoreceptors or RPE and transduce just a fraction of the retina, they are suitable only for the treatment of rare degenerative diseases in which retinal structures remain intact. To address the need for broadly applicable gene delivery approaches, we implemented in vivo-directed evolution to engineer AAV variants that deliver the gene cargo to the outer retina after injection into the eyes easily accessible vitreous humor. This approach has general implications for situations in which dense tissue penetration poses a barrier for gene delivery. A resulting AAV variant mediated ...
LeDoux J. Gene Therapy Protocols: Volume 1: Production and In Vivo Applications of Gene Transfer Vectors (Methods in...LeDoux J. Gene Therapy Protocols: Volume 1: Production and In Vivo Applications of Gene Transfer Vectors (Methods in...
This book is great to learn, produce, and apply several different types of viral and non-viral vectors for gene transfer. It provides all necessary information from beginning to the end for successful genaration of vectors. Troubleshooting guidelines is also very helpful ...
Regulation of Transgene Expression in Tumor Cells by Exploiting Endogenous Intracellular Signals | Nanoscale Research Letters |...Regulation of Transgene Expression in Tumor Cells by Exploiting Endogenous Intracellular Signals | Nanoscale Research Letters |...
Gene therapy is recognized as a medical approach for the treatment of diseases that are difficult to cure, such as tumors. Several viral and non-viral carriers for gene transfer have been developed for either in vivo or ex vivo/in vitro use [1-4]. The most important viral carriers, characterized in laboratory studies and clinical trials, have been inactivated retroviruses, adenoviruses, adeno-associated viruses, and herpes viruses. These viruses show relatively high transfection efficiency, but have some clinical safety problems. On the other hand, non-viral methods of gene delivery, including cationic lipofection, calcium phosphate precipitation, gene guns, and injection of naked DNA, generally show low transfection efficiency but are not pathogenic [1-4]. However, there is another serious issue regarding the present gene delivery methodologies. Almost all the gene delivery methods currently developed have insufficient ability to specifically recognize and target diseased cells and to ...
Molecular basis of Agrobacterium-mediated gene transfer into mammalian cells | ScholarBank@NUSMolecular basis of Agrobacterium-mediated gene transfer into mammalian cells | [email protected]
A novel system for detection of gene transfer between A. tumefaciens and mammalian cells was established. Using this system, Agrobacterium was found to be able to deliver DNA located on its plasmid and chromosome into human cells. Agrobacterium is actively involved in this process; such a kind of DNA transfer might occur widely between bacteria and mammalian cells. The frequency of such a gene transfer was approximately 10-4-10-5 transformants per recipient. Our data showed that this DNA transfer is dependent upon Agrobacterium and independent of its virulence genes. The polymerization of actin played an important role in Agrobacterium internalization and subsequent gene transfer into mammalian cells. In addition, an Agrobacterium sensor protein ChvG was found to be important for the gene transfer into both plant and mammalian cells. The data suggested that ChvG was involved in the regulation of acid-inducible genes and might function as a global sensor protein that can directly or indirectly ...
VEGF Gene Transfer for Diabetic Neuropathy - Full Text View - ClinicalTrials.govVEGF Gene Transfer for Diabetic Neuropathy - Full Text View - ClinicalTrials.gov
This gene therapy study is being conducted to evaluate intramuscular gene transfer using VEGF (Vascular Endothelial Growth Factor) in patients with diabetic neuropathy in the legs. This condition causes a decrease in feeling and sensation due to diabetes. VEGF is DNA, or genetic material that is injected into the muscles of the leg. Once in the leg, it has been shown to cause new blood vessels to grow under a variety of conditions ...
Ex vivo gene delivery of GDNF using primary astrocytes transdu...Ex vivo gene delivery of GDNF using primary astrocytes transdu...
Ex vivo gene delivery of GDNF using primary astrocytes transduced with a lentiviral vector provides neuroprotection in a rat model of Parkinsons disease.: Astr
About the Lab - Miyanohara Lab - UC San Diego Department of PediatricsAbout the Lab - Miyanohara Lab - UC San Diego Department of Pediatrics
Atsushi Miyanohara, Ph.D., is an experienced molecular biologist who has supervised the Vector Core Laboratory of the UCSD Program in Human Gene Therapy since its inception in 1995. He has longstanding extensive experience dealing with the design, preparation and properties of retrovirus, lentivirus, HSV1, adenovirus and AAV vectors. Dr. Miyanohara is one of the principals in the development of the VSV-G pseudotyping and VSV-G virosome technologies.. Dr. Miyanoharas major research focus has been development of safe and efficient gene transfer vectors not only for gene therapy applications, but also for a variety of basic research including stem cells, cell reprogramming and transgenesis.. As the Director of the Vector Core Laboratory, Dr. Miyanohara has extensive mutual research collaborations and has made important conceptual and technical advances in basic and clinical gene therapy, particularly in neurological and cardiovascular diseases.. Dr. Miyanohara received his Ph.D. in Medical ...
Glucocorticoid Priming of Nonviral Gene Delivery to hMSCs Increases Tr by Andrew Hamann, Tyler Kozisek et al."Glucocorticoid Priming of Nonviral Gene Delivery to hMSCs Increases Tr" by Andrew Hamann, Tyler Kozisek et al.
Human mesenchymal stem cells (hMSCs) are under study for cell and gene therapeutics because of their immunomodulatory and regenerative properties. Safe and efficient gene delivery could increase hMSC clinical potential by enabling expression of transgenes for control over factor production, behavior, and differentiation. Viral delivery is efficient but suffers from safety issues, while nonviral methods are safe but highly inefficient, especially in hMSCs. We previously demonstrated that priming cells with glucocorticoids (Gcs) before delivery of DNA complexes significantly increases hMSC transfection, which correlates with a rescue of transfection-induced metabolic and protein synthesis decline, and apoptosis. In this work, we show that transgene expression enhancement is mediated by transcriptional activation of endogenous hMSC genes by the cytosolic glucocorticoid receptor (cGR) and that transfection enhancement can be potentiated with a GR transcription-activation synergist. We demonstrate that the
The Tree of Life: Ugg - story about gene transfer/evolution based on NSF press release has a NASA-esque smellThe Tree of Life: Ugg - story about gene transfer/evolution based on NSF press release has a NASA-esque smell
This quote is deeply troubling. Genome sequencing did not reveal a new mechanism of evolution. And it is thus also inaccurate to say it would not have been discovered any other way". Lateral gene transfer was studies for many many many years before the first genome sequence was determined. Certainly, comparative genome analysis helped reveal the extent of gene transfer but it is seriously inaccurate to say it "revealed a new mechanism of evolution". Here for example is a link to a google search for the specific phrase "lateral gene transfer" in papers published prior to 1995. And here is one for the phrase "horizontal gene transfer ...
Robert Sapolsky - Wisdom ReelRobert Sapolsky - Wisdom Reel
Robert Morris Sapolsky (born 1957) is an American neuroendocrinologist and author. He is currently a professor of biology, and professor of neurology and neurological sciences and, by courtesy, neurosurgery, at Stanford University. In addition, he is a research associate at the National Museums of Kenya.. As a neuroendocrinologist, he has focused his research on issues of stress and neuronal degeneration, as well as on the possibilities of gene therapy strategies for protecting susceptible neurons from disease. Currently, he is working on gene transfer techniques to strengthen neurons against the disabling effects of glucocorticoids. Each year Sapolsky spends time in Kenya studying a population of wild baboons in order to identify the sources of stress in their environment, and the relationship between personality and patterns of stress-related disease in these animals. More specifically, Sapolsky studies the cortisol levels between the alpha male and female and the subordinates to determine ...
The MT 3000-2i from Vƶgele Offers Exceptional Performance in New-Design Material Transfer Vehicle :: Story ID: 26188 ::...The MT 3000-2i from Vƶgele Offers Exceptional Performance in New-Design Material Transfer Vehicle :: Story ID: 26188 ::...
The newly designed MT 3000-2i material transfer vehicle from Vƶgele offers exceptional performance in material transfer vehicles for asphalt paving. This machine is designed with the contractor in mind for unmatched productivity, exceptionally low operating costs and ultimate maneuverability. (ID::26188)
Enhancing gene delivery with nanoparticles could ultimately lead to a cure for AlzheimersEnhancing gene delivery with nanoparticles could ultimately lead to a cure for Alzheimer's
A number of neurodegenerative disorders, such as Parkinsons or Alzheimer Disease, may potentially be treated by gene therapy, i.e. the delivery of therapeutic genes to neurons. Currently, the most common carrier molecules to deliver the therapeutic gene to the patients target cells are viruses that have been genetically altered to carry normal human DNA. Overall gene delivery efficiency is typically low for nonviral vectors. New research undertaken at The Johns Hopkins University offers a systematic approach to understanding the gene delivery process in neurons and explores the intracellular barriers to nonviral gene delivery and possible ways to improve their effectiveness.
Mucosal IFN-gamma gene transfer inhibits pulmonary allergic responses in mice. | The Journal of ImmunologyMucosal IFN-gamma gene transfer inhibits pulmonary allergic responses in mice. | The Journal of Immunology
Th2 cytokines are associated with the airway inflammation and hyperreactivity in allergic asthma and are potential targets for developing novel therapies. The efficacy of a mucosal cytokine-gene transfer approach was examined in a mouse model for allergic asthma. We showed first that mucosal IFN-gamma gene transfer results in a significant expression of IFN-gamma in the pulmonary epithelium. Significantly, this approach inhibits both Ag- and Th2-cell-induced pulmonary eosinophilia and airway hyperreactivity. These findings suggest that mucosal IFN-gamma gene transfer is effective in modulating pulmonary allergic responses and provides a basis for developing a novel therapeutic approach. ...
Liposome-mediated target gene transfer into mouse endothelial cells in vivoLiposome-mediated target gene transfer into mouse endothelial cells in vivo
We have developed an endothelial specific gene delivery in vivo system (catalog number: EG6881) by combination of liposome mediated gene delivery and endothelial specific expression promoter vector to ensure target genes transferred in endothelial cells only for studying target gene functions in endothelial cell proliferation, differentiation, and death in vivo. The non-viral in vivo gene delivery system will be able to mediate endothelial-related target genes expressed only in endothelial cells. ...
Calcium-crosslinked LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cellsCalcium-crosslinked LABL-TAT complexes effectively target gene delivery to ICAM-1 expressing cells
Targeted gene delivery using non-viral vectors is a highly touted scheme to reduce the potential for toxic or immunological side effects by reducing dose. In previous reports, TAT polyplexes with DNA have shown relatively poor gene delivery. The transfection efficiency has been enhanced by condensing TAT/DNA complexes to a small particle size using calcium. To explore the targetability of these condensed TAT complexes, LABL peptide targeting intercellular cell-adhesion molecule-1 (ICAM-1) was conjugated to TAT peptide using a polyethylene glycol (PEG) spacer. PEGylation reduced the transfection efficiency of TAT, but TAT complexes targeting ICAM-1 expressing cells regained much of the lost transfection efficiency. Targeted block peptides properly formulated with calcium offer promise for gene delivery to ICAM-1 expressing cells at sites of injury or inflammation. ...
Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer | ProtocolPreparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer | Protocol
This protocol describes the method, materials, equipment and steps for bottom-up preparation of RNA and protein producing synthetic...
Advanced Textbook on Gene Transer, Gene Therapy and Genetic by Daniel Scherman - Novoline LibraryAdvanced Textbook on Gene Transer, Gene Therapy and Genetic by Daniel Scherman - Novoline Library
Indd 41 11/8/2013 12:05:31 PM B1574 Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology 42 Advanced Textbook on Gene Transfer, Gene Therapy and Genetic Pharmacology cause a reduction in expression if there are six to seven consecutive matches between the 5 end of siRNA guide strand and the off-target mRNA sequence. Some rules now included in web software for design of siRNA sequences attempt to ensure that this partial complementarity is avoided. The most critical non-sequence-specific effect of siRNAs is the induction of the immune response. 3 Non-target-related effects Unexpected effects of antisense oligonucleotides and more specifically oligophosphorothioates, unrelated to their RNA binding, have been described. These nonantisense effects can be both non-sequence-specific (resulting from the polyanionic nature of the oligonucleotides) or sequence specific. The non-antisense effects have been mainly described with unmodified oligonucleotides and with ...
Lipid polycation DNA gene delivery system - Targeted Genetics/Elan - AdisInsightLipid polycation DNA gene delivery system - Targeted Genetics/Elan - AdisInsight
Lipid Polycation DNA (LPD) is Targeted Genetics newest synthetic gene delivery system. Targeted Genetics, in collaboration with Dr L Huang at the University of
Fittest Of Newborn Neurons Survive ( Researchers at the Salk Institute for B...)Fittest Of Newborn Neurons Survive ( Researchers at the Salk Institute for B...)
Health,Researchers at the Salk Institute for Biological Studies say that even...The NMDA receptor is activated by the neurotransmitter glutamate ... When we removed the NMDA receptor that is when cells make connec...Combining mouse genetics and gene transfer techniques Gage and a ...A few weeks later animals that received the virus showed fewer fl...,Fittest,Of,Newborn,Neurons,Survive,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Robust Adenoviral and Adeno-Associated Viral Gene Transfer to the In Vivo Murine Heart | CirculationRobust Adenoviral and Adeno-Associated Viral Gene Transfer to the In Vivo Murine Heart | Circulation
Mice were anesthetized with isoflurane induction and endotracheally intubated. Animals were placed supine on a thermoregulated table (37Ā°C) and ventilated with isoflurane (2%) vaporized in 100% O2 using a custom constant-flow ventilator (6.7 Ī¼L/gāˆ’1 tidal volume at 120 minutesāˆ’1). ECG and core temperature were monitored. The left external jugular vein was cannulated, and modest volume expansion was provided (100 to 150 Ī¼L of 12.5% human albumin) at 30 Ī¼L mināˆ’1.. Topical 2% lidocaine gel was applied to the chest, and the thorax was entered by 1 of 2 techniques. For the first method, virus was injected into the LV chamber. Mice were placed on their right sides with the left forepaw affixed to the surgical table. The point of maximal intensity was visualized (approximately 0.5 to 0.75 cm below the axilla), and an incision was made to the left side of the point of maximal intensity. The thorax was carefully entered, avoiding cardiac injury, and the thoracotomy was widened by blunt ...
ASMscience | Gene Transfer in Gram-NeASMscience | Gene Transfer in Gram-Ne
One realization that has come from comparing multiple bacterial genome sequences, including multiple isolates from the same species, is that gene transfer is an important force in bacterial genome evolution. In the laboratory gene transfer is essential for the study of bacteria and for learning more about all living organisms. Three processes in bacteria can broadly define the transfer of DNA: transformation, transduction, and conjugation. This chapter focuses on the many genetic tools available to manipulate the genetic content of Escherichia coli. A DNA molecule that does not have its own origin of replication must integrate into either the host chromosome or another autonomously replicating element such as an endogenous plasmid. In E. coli a modified derivative of the bacteriophage T4 offers some advantages for transduction in that it packages twice as much DNA as P1 and also is less sensitive to capsules found on many pathogenic strains of E. coli. Transformation of bacteria by use of either
Patent US20010020011 - Polymeric gene delivery system - Google PatentsuchePatent US20010020011 - Polymeric gene delivery system - Google Patentsuche
A means for obtaining efficient introduction of exogenous genes into a patient, with long term expression of the gene, is disclosed. The gene, under control of an appropriate promoter for expression in a particular cell type, is encapsulated or dispersed with a biocompatible, preferably biodegradable polymeric matrix, where the gene is able to diffuse out of the matrix over an extended period of time, for example, a period of three to twelve months or longer. The matrix is preferably in the form of a microparticle such as a microsphere (where the gene is dispersed throughout a solid polymeric matrix) or microcapsule (gene is stored in the core of a polymeric shell), a film, an implant, or a coating on a device such as a stent. The size and composition of the polymeric device is selected to result in favorable release kinetics in tissue. The size is also selected according to the method of delivery which is to be used, typically injection or administration of a suspension by aerosol into the nasal and/or
Patent US20010020011 - Polymeric gene delivery system - Google PatentsPatent US20010020011 - Polymeric gene delivery system - Google Patents
A means for obtaining efficient introduction of exogenous genes into a patient, with long term expression of the gene, is disclosed. The gene, under control of an appropriate promoter for expression in a particular cell type, is encapsulated or dispersed with a biocompatible, preferably biodegradable polymeric matrix, where the gene is able to diffuse out of the matrix over an extended period of time, for example, a period of three to twelve months or longer. The matrix is preferably in the form of a microparticle such as a microsphere (where the gene is dispersed throughout a solid polymeric matrix) or microcapsule (gene is stored in the core of a polymeric shell), a film, an implant, or a coating on a device such as a stent. The size and composition of the polymeric device is selected to result in favorable release kinetics in tissue. The size is also selected according to the method of delivery which is to be used, typically injection or administration of a suspension by aerosol into the nasal and/or
Patent US20010020011 - Polymeric gene delivery system - Google PatentsPatent US20010020011 - Polymeric gene delivery system - Google Patents
A means for obtaining efficient introduction of exogenous genes into a patient, with long term expression of the gene, is disclosed. The gene, under control of an appropriate promoter for expression in a particular cell type, is encapsulated or dispersed with a biocompatible, preferably biodegradable polymeric matrix, where the gene is able to diffuse out of the matrix over an extended period of time, for example, a period of three to twelve months or longer. The matrix is preferably in the form of a microparticle such as a microsphere (where the gene is dispersed throughout a solid polymeric matrix) or microcapsule (gene is stored in the core of a polymeric shell), a film, an implant, or a coating on a device such as a stent. The size and composition of the polymeric device is selected to result in favorable release kinetics in tissue. The size is also selected according to the method of delivery which is to be used, typically injection or administration of a suspension by aerosol into the nasal and/or
DI-fusion Efficient gene transfer to dispersed human pancreatic islet...DI-fusion Efficient gene transfer to dispersed human pancreatic islet...
DI-fusion, le DĆ©pĆ“t institutionnel numĆ©rique de lULB, est loutil de rĆ©fĆ©rencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thĆØses qui y ont Ć©tĆ© dĆ©fendues.
Researchers determine where Canadian data transfers increase exposure to US state surveillanceResearchers determine where Canadian data transfers increase exposure to US state surveillance
Researchers at the University of Toronto announced today that IXmaps, a visual, interactive database of Internet traffic routes, is now live. The tool, funded by the .CA Community Investment Program, helps Canadians understand ...
Engineered AAVs for Efficient Noninvasive Gene Delivery to the Central and Peripheral Nervous SystemsEngineered AAVs for Efficient Noninvasive Gene Delivery to the Central and Peripheral Nervous Systems
Adeno-associated viruses (AAVs) are commonly used for in vivo gene transfer. Nevertheless, AAVs that provide efficient transduction across specific organs or cell populations are needed. Here, we describe AAV-PHP.eB and AAV-PHP.S, capsids that efficiently transduce the central and peripheral nervous ā€¦
Exosomes: Therapy delivery tools and biomarkers of diseasesExosomes: Therapy delivery tools and biomarkers of diseases
Virtually all cells in the organism secrete extracellular vesicles (EVs), a heterogeneous population of lipid bilayer membrane-enclosed vesicles that transport and deliver payloads of proteins and nucleic acids to recipient cells, thus playing central roles in cell-cell communications. Exosomes, nan ā€¦
Bead transfection of adherent cells. Gene transfer into adhere...Bead transfection of adherent cells. Gene transfer into adhere...
Bead transfection of adherent cells. Gene transfer into adherent mammalian cells using glass beads.: Bead transfection is a simple, rapid, efficient, and cost-e
Genetics: Mutation, Cell Division and Chromosomes/The Transmission Genetics of Eukaryotes/Gene Transfer in Bacteria and...Genetics: Mutation, Cell Division and Chromosomes/The Transmission Genetics of Eukaryotes/Gene Transfer in Bacteria and...
Genetics: Mutation, Cell Division and Chromosomes/The Transmission Genetics of Eukaryotes/Gene Transfer in Bacteria and Bacteriophages Unit 3-5, 9780335163014, available at Book Depository with free delivery worldwide.
Antibacterial gene transfer across the tree of life | eLifeAntibacterial gene transfer across the tree of life | eLife
Parallel horizontal gene transfer has spread a bacteriolytic gene family to all domains of life, and has bestowed a niche-transcending adaptation in recipients that must deploy antibacterial molecules to survive in a bacterial world.
Is it time to replace SMTP? | ZDNetIs it time to replace SMTP? | ZDNet
The industry first adopted the Simple Mail Transfer Protocol (SMTP) standard long before the Internet became a ubiquitous form of communication. Since then, numerous threats have emerged, but SMTP has remained the same. Jonathan Yarden explains why he thinks the time has come to find a replacement for SMTP and proposes a solution.
FDA Approves Novartis CAR-T Drug, First Gene Therapy Approval in US | Pharmaceutical TechnologyFDA Approves Novartis' CAR-T Drug, First Gene Therapy Approval in US | Pharmaceutical Technology
FDA approves Novartis CAR-T therapy, marking the first time a cell therapy based on gene transfer has been approved in the United States for any indication.
results : FAO in Emergenciesresults : FAO in Emergencies
Many vulnerable rural families in Iraq can now benefit from a safer, more secure means of receiving income thanks to mobile money transfer technology adopted for the first ...read more ...
DNA Delivery and Protein Synthesis | Free Course | AlisonDNA Delivery and Protein Synthesis | Free Course | Alison
Learn about DNA Delivery and Protein Synthesis. Topics cover DNA delivery, screening of recombinant clones, protein synthesis machinery, and expression systems.
KAKEN - Researchers | ADACHI Masashi (00253526)KAKEN - Researchers | ADACHI Masashi (00253526)
Affiliationļ¼šMEDICAL INSTITUTE OF BIOREGULATION,LECTURE,ē”Ÿä½“防徔医学ē ”ē©¶ę‰€,åŠ©ę‰‹, Research Fieldļ¼šGeneral surgery, Keywordsļ¼šéŗ伝子ē§»å…„,ćƒ¬ćƒˆćƒ­ć‚¦ć‚£ćƒ«ć‚¹ćƒ™ć‚Æć‚æćƒ¼,ćƒ¬ćƒˆćƒ­ć‚¦ć‚¤ćƒ«ć‚¹ćƒ™ć‚Æć‚æćƒ¼,éŗä¼å­ę²»ē™‚,ć‚¤ćƒ³ć‚æćƒ¼ćƒŖćƒ„ćƒ¼ć‚­ćƒ³2,ēœŸę øē“°čƒžē™ŗē¾ćƒ™ć‚Æć‚æćƒ¼,ć‚µć‚¤ćƒˆć‚«ć‚¤ćƒ³,Gene transfer,Retroviral vector,ē½®Gene transfer, # of Research Projectsļ¼š1, # of Research Productsļ¼š0
Ligation (molecular biology)Ligation (molecular biology)
Genetically modified cropsGenetically modified crops
Genetically modified cropsGenetically modified crops
Seed trapSeed trap
Streptomyces vinaceusStreptomyces vinaceus
Conservative transpositionConservative transposition
Robert SapolskyRobert Sapolsky
Mitochondrial replacement therapyMitochondrial replacement therapy
Rafi Ahmed Kidwai AwardRafi Ahmed Kidwai Award
Sodium-iodide symporterSodium-iodide symporter
BiopharmaceuticalBiopharmaceutical
PhylogenomicsPhylogenomics
Influenza researchInfluenza research
Sharp Memorial HospitalSharp Memorial Hospital
Elizabeth NabelElizabeth Nabel
Ancestral reconstructionAncestral reconstruction
CisgenesisCisgenesis
Blotto (biology)Blotto (biology)
Molecular phylogeneticsMolecular phylogenetics
Genetically modified cropsGenetically modified crops
Marker-assisted selectionMarker-assisted selection
TriticaleTriticale
Spinocerebellar ataxiaSpinocerebellar ataxia
TransposaseTransposase
Hfr cellHfr cell
TransfectionTransfection
BiochemistryBiochemistry
BacteriaBacteria
Lipid bilayerLipid bilayer
Ralph L. BrinsterRalph L. Brinster
Saint Barnabas Medical CenterSaint Barnabas Medical Center
AcneAcne
Listener fatigueListener fatigue
Genetically modified tomatoGenetically modified tomato
Alkali metalAlkali metal
Cold fusionCold fusion
InbreedingInbreeding
RAD51RAD51
ArchaeaArchaea
Geologic time scaleGeologic time scale
FluoresceinFluorescein
Chronic obstructive pulmonary diseaseChronic obstructive pulmonary disease
TranslationTranslation
Familial hypercholesterolemiaFamilial hypercholesterolemia
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Rekayasa genetika bahasa Indonesia, ensiklopedia bebasRekayasa genetika bahasa Indonesia, ensiklopedia bebas
Solar cellSolar cell
PaleontologyPaleontology
Erinevus lehekĆ¼lje Transformatsioon (geneetika) redaktsioonide vahel - VikipeediaErinevus lehekĆ¼lje "Transformatsioon (geneetika)" redaktsioonide vahel - Vikipeedia
LoudspeakerLoudspeaker
Radiation therapyRadiation therapy
Plant virusPlant virus
Surface-enhanced Raman spectroscopySurface-enhanced Raman spectroscopy
TomatoTomato
FamineFamine
Dual inheritance theoryDual inheritance theory
EpigeneticsEpigenetics
Bed bugBed bug
LactationLactation
Open accessOpen access
AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesInformation ScienceHealth Care
Gene Transfer TechniquesMoving and Lifting PatientsGenetic VectorsGenetic TherapyTransfectionEmbryo TransferFluorescence Resonance Energy TransferBioluminescence Resonance Energy Transfer TechniquesGene Transfer, HorizontalEnergy TransferAdenoviridaeCell LineCells, CulturedPregnancyTransduction, GeneticNuclear Transfer TechniquesDependovirusRetroviridaeTransgenesbeta-GalactosidaseGene ExpressionPlasmidsPhylogenyTransfer (Psychology)ElectroporationMolecular Sequence Data
Download Gene Delivery To Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques 2004Download Gene Delivery To Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques 2004
Structural Biochemistry/Nucleic Acid/DNA/Transgenic Animals - Wikibooks, open books for an open worldStructural Biochemistry/Nucleic Acid/DNA/Transgenic Animals - Wikibooks, open books for an open world
The Impact of Gene Transfer Techniques in Eukaryotic Cell Biology | SpringerLinkThe Impact of Gene Transfer Techniques in Eukaryotic Cell Biology | SpringerLink
In vivo nasal potential difference: techniques and protocols for assessing efficacy of gene transfer in cystic fibrosis. -...In vivo nasal potential difference: techniques and protocols for assessing efficacy of gene transfer in cystic fibrosis. -...
NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.(Statistical Data Included) - Bulletin...NEW TECHNIQUES FOR ANALYSIS OF MICROBIAL GENE TRANSFER WITHIN A RHABDITIS SOIL NEMATODE.(Statistical Data Included) - Bulletin...
Gene Transfer Technique - Mybiosource Learning CenterGene Transfer Technique - Mybiosource Learning Center
Horizontal gene transfer - WURHorizontal gene transfer - WUR
Gene Delivery to Mammalian Cells | SpringerLinkGene Delivery to Mammalian Cells | SpringerLink
Top 6 Techniques of Gene Transfer | Genetic EngineeringTop 6 Techniques of Gene Transfer | Genetic Engineering
The Impact Of Gene Transfer Techniques In Eukaryotic Cell BiologyThe Impact Of Gene Transfer Techniques In Eukaryotic Cell Biology
New therapeutic approaches based on gene transfer techniques - Fingerprint - Mayo ClinicNew therapeutic approaches based on gene transfer techniques - Fingerprint - Mayo Clinic
Recent Articles | Horizontal Gene Transfer And Genetics & Genomics | The Scientist MagazineĀ®| Page 3Recent Articles | Horizontal Gene Transfer And Genetics & Genomics | The Scientist MagazineĀ®| Page 3
Recent Articles | Horizontal Gene Transfer, Immunology And Developmental Biology | The Scientist MagazineĀ®Recent Articles | Horizontal Gene Transfer, Immunology And Developmental Biology | The Scientist MagazineĀ®
Eukaryotic Gene Transfer and the New Genetics | The American Journal of Tropical Medicine and HygieneEukaryotic Gene Transfer and the New Genetics | The American Journal of Tropical Medicine and Hygiene
Bacterial survival in the host and in the environment is promoted by horizontal gene transfer of additional metal resistance...Bacterial survival in the host and in the environment is promoted by horizontal gene transfer of additional metal resistance...
Horizontal Gene TransferHorizontal Gene Transfer
Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques | BMC Biotechnology ...Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques | BMC Biotechnology ...
Gene Therapy in HIV-Positive Patients With Non-Hodgkin's Lymphoma - Full Text View - ClinicalTrials.govGene Therapy in HIV-Positive Patients With Non-Hodgkin's Lymphoma - Full Text View - ClinicalTrials.gov
Evaluating the Safety and Effectiveness of Mozobil Mobilization in Adults With Beta-Thalassemia Major - Full Text View -...Evaluating the Safety and Effectiveness of Mozobil Mobilization in Adults With Beta-Thalassemia Major - Full Text View -...
BGI Sequencing news: German EHEC strain is a chimera created by horizontal gene transfer - Biology Fortified Inc.BGI Sequencing news: German EHEC strain is a chimera created by horizontal gene transfer - Biology Fortified Inc.
In vivo effects of hepatocyte growth factor/scatter factor on mouse mammary gland developmentIn vivo effects of hepatocyte growth factor/scatter factor on mouse mammary gland development
Safety and efficacy of gene transfer for Lebers congenital amaurosisSafety and efficacy of gene transfer for Leber's congenital amaurosis
Maurizio Labbate | University of Technology SydneyMaurizio Labbate | University of Technology Sydney
EXTOXNET FAQs - What is Genetic Engineering?EXTOXNET FAQs - What is Genetic Engineering?
How Crispr Could Transform Our Food SupplyHow Crispr Could Transform Our Food Supply
Transgenic Animals: 1st Edition (Paperback) - RoutledgeTransgenic Animals: 1st Edition (Paperback) - Routledge
Using a gene transfer technique to study the role of endothelial nitric oxide synthase in neovascularization and myocardial...Using a gene transfer technique to study the role of endothelial nitric oxide synthase in neovascularization and myocardial...
Phys.org - gene transferPhys.org - gene transfer
Phys.org - horizontal gene transferPhys.org - horizontal gene transfer
CRISPR/Cas9/sgRNA-mediated targeted gene modification confirms the cause-effect relationship between gyrA mutation and...CRISPR/Cas9/sgRNA-mediated targeted gene modification confirms the cause-effect relationship between gyrA mutation and...
Doxorubicin as a molecular nanotheranostic agent: effect of doxorubicin encapsulation in micelles or nanoemulsions on the...Doxorubicin as a molecular nanotheranostic agent: effect of doxorubicin encapsulation in micelles or nanoemulsions on the...
AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesInformation ScienceHealth Care
Gene Transfer TechniquesMoving and Lifting PatientsGenetic VectorsGenetic TherapyTransfectionEmbryo TransferFluorescence Resonance Energy TransferBioluminescence Resonance Energy Transfer TechniquesGene Transfer, HorizontalEnergy TransferAdenoviridaeCell LineCells, CulturedPregnancyTransduction, GeneticNuclear Transfer TechniquesDependovirusRetroviridaeTransgenesbeta-GalactosidaseGene ExpressionPlasmidsPhylogenyTransfer (Psychology)ElectroporationMolecular Sequence Data
VectorsExpressionGenomeTherapyMiceRegulationTransductionMethodsEfficiencyCellsMethod of gene transferEmbryoVivo gene transferForeign geneScientistsDeliveryHumansBacteriaHorizontal gene traProteinsMicrobialLaboratoryMolecularSpeciesForeign genesApproachesGeneticallyDiseasesResistancePlantChromosomalCropsNematodeEfficacyConjugationLateral gene traOrganismsSequencesPromotersTRNAScientistsHybridizationBacteriumVivoPlantsLineagesViral and non-viralInsulin
Vectors1
Expression3
Genome3
  • Transgenic animal are animals that have had foreign genes from another animal introduced into their genome. (wikibooks.org)
  • With the newfound discovery of the DNA sequence of the human genome, scientists can now study the genes that are involved as drug targets, which can help provide them with the ability to mark the appropriate gene that can aid in providing the cure to any certain disease that they are studying. (wikibooks.org)
  • After fertilization of the egg, the DNA is shown to be successfully integrated into the genome of viable pig and mouse offspring with germ-line transfer to the F1 generation at a highly efficient rate: 37.5% of pigs and 33% of mice. (biomedcentral.com)
Therapy2
Mice1
Regulation1
Transduction1
Methods1
Efficiency2
Cells1
  • Cells are then able to integrate with the transgene, and the foreign gene is expressed, upon which the developing embryo is surgically implanted in a surrogate mother. (wikibooks.org)
Method of gene transfer1
  • The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. (biomedcentral.com)
Embryo1
  • The result of this process, if the embryo develops, is a transgenic animal housing a particular gene from another species. (wikibooks.org)
Vivo gene transfer1
Foreign gene2
  • A foreign gene (such as a hormone or blood protein) is cloned and injected into the nuclei of another animal's in vitro fertilized egg. (wikibooks.org)
  • Microinjection is the preferred method for introduction of a foreign gene into the mouse, a reliable technique developed by Gordon and his colleagues in 1980 [ 1 ]. (biomedcentral.com)
Scientists1
  • Animals that have their genes modified to show disease symptoms, may be studied and cure could possibly be contrived in the near future.At Harvard, scientists created a transgenic mouse also known as OncoMouseĀ® or also known as the Harvard mouse which allows it to carry genes that can enhance the development of a variety of cancers that are found in humans. (wikibooks.org)
Delivery1
Humans1
  • Due to worldwide shortages of organs, advances in gene manipulation of animals can alter their organs to become susceptible to humans. (wikibooks.org)
Bacteria20
Horizontal gene tra6
Proteins9
  • It has become an indispensable tool for gene cloning, the study of gene function and regulation and the production of small amounts of recombinant proteins for analysis and verification. (mybiosource.com)
  • Such animals are used to study gene function and expression, model human diseases, produce recombinant proteins in their milk and other fluids, and to improve the quality of livestock herds and other domestic species. (mybiosource.com)
  • Besides this, there controls a download Gene Delivery to that any surface for a coil, proteins state, broad, or additional Glycogen possibly starts as a recruit for distinct cGMP. (evakoch.com)
  • If they advocate Additionally regulated, a download Gene Delivery to Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques 2004 in which activated SI plays a competent vitamin, they are in the protein loop and remain reviewed by I proteins, involving to major and extracellular face( Naim et al. (evakoch.com)
  • It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. (routledge.com)
  • eNOS gene delivery resulted in suppression of oxidative stress induced by MI which led to decreases in TGF-beta1 and p27 expression , INK activation , and nuclear translocation of NF-kappaB , proteins known to be induced by reactive oxygen species . (musc.edu)
  • Because amino acids of proteins that are functionally important are locally clustered in domains, mutations in multiple tumors that are functionally important to the development of cancer cluster in the linear sequence of relevant genes, allowing inference of relevance and function even in cases without three-dimensional protein structure. (yale.edu)
  • In 1987, Plant Genetic Systems ( Ghent , Belgium ), founded by Marc Van Montagu and Jeff Schell , was the first company to genetically engineer insect-resistant ( tobacco ) plants by incorporating genes that produced insecticidal proteins from Bacillus thuringiensis (Bt). (wikipedia.org)
  • This technique allows us to study the modification of chromatin histone proteins (acetylation/methylation) at different stages of viral gene expression. (upenn.edu)
Microbial1
Laboratory6
Molecular10
Species7
  • If these undifferentiated callus cell-clumps are subsequently (i.e., within a year) transferred to growth medium favoring differentiation (medium lacking 2, 4-D hormone but containing growth hormones such as kinetin), plantlet will regenerate in case of many, but not all, species. (biologydiscussion.com)
  • Several natural mechanisms allow gene flow across species. (wikipedia.org)
  • Multiple natural mechanisms allow gene flow from one species to another. (wikipedia.org)
  • Because the method of introduction is a physical one, the biological barriers that restrict the application of other DNA transfer methods to a few plant species and a few cell types are not present. (freepatentsonline.com)
  • This rooting suggests that the dsrAB genes in Archaeoglobus species also are the result of an ancient lateral transfer from a bacterial donor. (asm.org)
  • The current method of gene transfer, microinjection, which is widely used in transgenic mouse production, has only had limited success in producing transgenic animals of larger or higher species. (nih.gov)
  • We found that transferred genes are concentrated in only ~1% of the chromosomal regions (hotspots) in 80 bacterial species. (pasteur.fr)
Foreign genes1
Approaches8
  • While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. (routledge.com)
  • The-state-of-art CRISPR/Cas9 is one of the most powerful among the approaches being developed to rescue fundamental causes of gene-based inheritable diseases. (bioportfolio.com)
  • First, we are applying maximum likelihood approaches that we have developed for model-averaged clustering in discrete linear sequences to somatic amino acid replacement mutations appearing within mutated genes. (yale.edu)
  • This position requires experience with murine or human hematopoietic stem cell (HSC) biology in order to establish in vitro and in vivo models needed for the characterization of product candidates together with experience in evaluating diverse approaches for gene delivery into HSC to examine the regulation of hematopoiesis in transplant models. (virology.net)
  • After 30 years of focusing on optimizing successful gene delivery, it is very rewarding to finally see these approaches being tested for some of the unmet clinical needs caused by these terminal genetic disorders," Samulski said. (healthcanal.com)
  • Providing a broad coverage of the field, the authors cover fundamental and technical information on these techniques and approaches, as well as discussing their related problems. (wiley.com)
  • Techniques and problem-solving approaches will be the focus of multiple lectures and will be integrated into classroom discussions. (umn.edu)
  • Students will be exposed to a rapidly expanding body of knowledge, as well as an interdisciplinary approach to investigation that includes the integration of cutting-edge techniques and approaches from traditionally disparate fields. (umn.edu)
Genetically4
  • In this paper, we argue that lesbian couples who wish to have children who are genetically related to both of them should be allowed access to mitochondrial replacement techniques (MRTs). (bmj.com)
  • The latter techniques help women wishing to become mothers who carry mitochondrial DNA (mtDNA) abnormalities in their eggs to have genetically related offspring free from mtDNA diseases. (bmj.com)
  • Genetically engineered "knock-out" pigs that lack the a1,3-galactosyltransferase gene (GalT-KO) and thus do not express the Gal oligosaccharide have been used to improve the hyperacute rejection (Hisashi et al. (thefreelibrary.com)
  • The treatment approach developed at UNC uses a genetically modified virus called AAV to deliver a missing gene, the gigaxonin gene (scAAV9/JeT-GAN), into the cerebrospinal fluid of children with GAN. (healthcanal.com)
Diseases5
  • Due to her background and interests, Phoebe mostly writes for the Life Sciences side of News-Medical, focussing on Microbiology and related techniques and diseases. (news-medical.net)
  • In medicine, gene editing could potentially cure inherited diseases, such as some forms of heart disease and cancer and a rare disorder that causes vision loss. (nationalgeographic.com)
  • V gene analysis also revealed that the V3-7 or VH4-21 gene, which encodes nephritogenic anti-DNA Ab O-81 or NE-1, was preferentially used for IgG anti-DNA autoantibodies or IgG anti-acetylcholine receptor Abs associated with autoimmune diseases, but not for the Abs to exogenous Ags ( 7 , 8 , 9 , 10 ). (jimmunol.org)
  • A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. (labome.org)
  • This is the first gene delivery approach directly into the spinal fluid in order to treat an inherited neurological disorder, and is expected to pave the way to developing treatments for many other related diseases. (healthcanal.com)
Resistance13
Plant2
Chromosomal1
Crops2
  • In agriculture, the technique can create plants that not only produce higher yields, like Lippman's tomatoes, but also ones that are more nutritious and more impervious to drought and pests, traits that may help crops endure more extreme weather patterns predicted in the coming years. (nationalgeographic.com)
  • For example, at the basic level, agronomists use sophisticated techniques to unravel the genetic makeup of major crops in order to change their adaptation, nutritive value, or to breed medicinal benefits into agronomic crops. (encyclopedia.com)
Nematode2
  • The success rate of infection and development of the nematode can give us clues for the function of a gene. (wur.nl)
  • In this way we are able to silence nematode genes that are putative parasitism related, and study their mode of action. (wur.nl)
Efficacy2
Conjugation1
Lateral gene tra1
  • Evidence for a dsrAB lateral gene transfer event also was found within the Ī“- Proteobacteria, affecting Desulfobacula toluolica . (asm.org)
Organisms4
Sequences1
  • The German outbreak strain is a new strain which has acquired specific gene sequences that have a role in pathogenicity, causing hemorrhagic colitis and hemolytic-uremic syndrome (HUS) . (biofortified.org)
Promoters1
  • Special promoters were inserted along with the gene so that the sugar is produced only when the rice plants need it. (latimes.com)
TRNA3
  • The human DNA segment in Ī»htX-l contains five or more presumptive tRNA genes and at least one Alu family member. (unt.edu)
  • The 19-kilobase human DNA insert in Ī»htX-2 contains two or more presumptive tRNA genes and at least three Alu family members. (unt.edu)
  • The 18.5-kilobase human DNA fragment in Ī»hVKV7 contains a cluster of three tRNA genes and at least nine Alu family members. (unt.edu)
Scientists4
  • Scientists first identify a gene responsible for a trait. (nationalgeographic.com)
  • Concentrating on a gene known as ALK the scientists used a small-mole. (bio-medicine.org)
  • Concentrating on a gene known as ALK, the scientists used a small-molecule inhibitor a technique common to many drugs to block abnormalities that apparently cause neuroblastomas. (bio-medicine.org)
  • Led by Dana-Farber Cancer Institute researchers Rani E. George, M.D., Ph.D., an assistant professor of pediatrics at Harvard Medical School, and A. Thomas Look, M.D., a professor of pediatrics at Harvard, the scientists analyzed the ALK gene in 94 tumors representative of general neuroblastomas and 30 neuroblastoma cell lines. (bio-medicine.org)
Hybridization2
Bacterium1
Vivo4
  • This paper describes techniques that can be used to discriminate in vivo between the ion transport phenotype of normal subjects and patients with cystic fibrosis. (nih.gov)
  • Bioluminescence was measured using an In Vivo Imaging System (IVIS-50) at various time-points following transfer. (biomedcentral.com)
  • The results presented in this ex vivo system may be a first step toward expressing genes with products that could be continuously delivered to the eye through the tears. (curehunter.com)
  • We applied this marking technique to the study of lineage relationships in the developing vertebrate nervous system, both in vivo and in culture. (pnas.org)
Plants4
Lineages1
  • The presence of dsrAB homologs in at least five highly divergent prokaryotic lineages and overall phylogenetic congruence of the dsrAB tree with the 16S rRNA gene tree suggested that the dissimilatory sulfite reductases of extant SRPs evolved vertically from common ancestral protogenotic genes ( 35 ). (asm.org)
Viral and non-viral1
Insulin1