Factor derived from leukocyte lysates of immune donors which can transfer both local and systemic cellular immunity to nonimmune recipients.
The introduction of functional (usually cloned) GENES into cells. A variety of techniques and naturally occurring processes are used for the gene transfer such as cell hybridization, LIPOSOMES or microcell-mediated gene transfer, ELECTROPORATION, chromosome-mediated gene transfer, TRANSFECTION, and GENETIC TRANSDUCTION. Gene transfer may result in genetically transformed cells and individual organisms.
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
The amount of a gas taken up, by the pulmonary capillary blood from the alveolar gas, per minute per unit of average pressure of the gradient of the gas across the BLOOD-AIR BARRIER.
Resistance to a disease-causing agent induced by the introduction of maternal immunity into the fetus by transplacental transfer or into the neonate through colostrum and milk.
Candidiasis of the skin manifested as eczema-like lesions of the interdigital spaces, perleche, or chronic paronychia. (Dorland, 27th ed)
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Carbon monoxide (CO). A poisonous colorless, odorless, tasteless gas. It combines with hemoglobin to form carboxyhemoglobin, which has no oxygen carrying capacity. The resultant oxygen deprivation causes headache, dizziness, decreased pulse and respiratory rates, unconsciousness, and death. (From Merck Index, 11th ed)
The volume of air contained in the lungs at the end of a maximal inspiration. It is the equivalent to each of the following sums: VITAL CAPACITY plus RESIDUAL VOLUME; INSPIRATORY CAPACITY plus FUNCTIONAL RESIDUAL CAPACITY; TIDAL VOLUME plus INSPIRATORY RESERVE VOLUME plus functional residual capacity; or tidal volume plus inspiratory reserve volume plus EXPIRATORY RESERVE VOLUME plus residual volume.
Phenomenon of cell-mediated immunity measured by in vitro inhibition of the migration or phagocytosis of antigen-stimulated LEUKOCYTES or MACROPHAGES. Specific CELL MIGRATION ASSAYS have been developed to estimate levels of migration inhibitory factors, immune reactivity against tumor-associated antigens, and immunosuppressive effects of infectious microorganisms.
Techniques and strategies which include the use of coding sequences and other conventional or radical means to transform or modify cells for the purpose of treating or reversing disease conditions.
An increased reactivity to specific antigens mediated not by antibodies but by cells.
The exchange of OXYGEN and CARBON DIOXIDE between alveolar air and pulmonary capillary blood that occurs across the BLOOD-AIR BARRIER.
A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases.
Measurement of the various processes involved in the act of respiration: inspiration, expiration, oxygen and carbon dioxide exchange, lung volume and compliance, etc.
The volume of air that is exhaled by a maximal expiration following a maximal inspiration.
Epicutaneous or intradermal application of a sensitizer for demonstration of either delayed or immediate hypersensitivity. Used in diagnosis of hypersensitivity or as a test for cellular immunity.
Measurement of the amount of air that the lungs may contain at various points in the respiratory cycle.
Pollutants, present in soil, which exhibit radioactivity.
A mixture of the enzymes (streptokinase and streptodornase) produced by hemolytic streptococci. It is used topically on surface lesions and by instillation in closed body cavities to remove clotted blood or fibrinous or purulent accumulations. It is also used as a skin test antigen in evaluating generalized cell-mediated immunodeficiency. (Dorland, 27th ed) EC 3.-.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
A rare, X-linked immunodeficiency syndrome characterized by ECZEMA; LYMPHOPENIA; and, recurrent pyogenic infection. It is seen exclusively in young boys. Typically, IMMUNOGLOBULIN M levels are low and IMMUNOGLOBULIN A and IMMUNOGLOBULIN E levels are elevated. Lymphoreticular malignancies are common.
Protein factor(s) released by sensitized lymphocytes (and possibly other cells) that inhibit the movement of LEUKOCYTES, especially polymorphonuclear cells, away from their site of release. Assays for these factors are used as tests for cellular immunity. Two of the common assays are the LEUKOCYTE MIGRATION CAPILLARY TUBE TECHNIQUE (LMCT) and the LEUKOCYTE MIGRATION AGAROSE TEST (LMAT).
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
The volume of air remaining in the LUNGS at the end of a maximal expiration. Common abbreviation is RV.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
A protein extracted from boiled culture of tubercle bacilli (MYCOBACTERIUM TUBERCULOSIS). It is used in the tuberculin skin test (TUBERCULIN TEST) for the diagnosis of tuberculosis infection in asymptomatic persons.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
Vertical transmission of hereditary characters by DNA from cytoplasmic organelles such as MITOCHONDRIA; CHLOROPLASTS; and PLASTIDS, or from PLASMIDS or viral episomal DNA.
A sterile solution containing the by-products of growth products of COCCIDIOIDES IMMITIS, injected intracutaneously as a test for COCCIDIOIDOMYCOSIS.
Measure of the maximum amount of air that can be expelled in a given number of seconds during a FORCED VITAL CAPACITY determination . It is usually given as FEV followed by a subscript indicating the number of seconds over which the measurement is made, although it is sometimes given as a percentage of forced vital capacity.
A genus of the family PARVOVIRIDAE, subfamily PARVOVIRINAE, which are dependent on a coinfection with helper adenoviruses or herpesviruses for their efficient replication. The type species is Adeno-associated virus 2.
A process in which normal lung tissues are progressively replaced by FIBROBLASTS and COLLAGEN causing an irreversible loss of the ability to transfer oxygen into the bloodstream via PULMONARY ALVEOLI. Patients show progressive DYSPNEA finally resulting in death.
Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).
Pathological processes involving any part of the LUNG.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.
A mitosporic fungal genus which causes COCCIDIOIDOMYCOSIS.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
A form of pneumoconiosis caused by inhalation of asbestos fibers which elicit potent inflammatory responses in the parenchyma of the lung. The disease is characterized by interstitial fibrosis of the lung, varying from scattered sites to extensive scarring of the alveolar interstitium.
A naphthacene antibiotic that inhibits AMINO ACYL TRNA binding during protein synthesis.
Enlargement of air spaces distal to the TERMINAL BRONCHIOLES where gas-exchange normally takes place. This is usually due to destruction of the alveolar wall. Pulmonary emphysema can be classified by the location and distribution of the lesions.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Curves depicting MAXIMAL EXPIRATORY FLOW RATE, in liters/second, versus lung inflation, in liters or percentage of lung capacity, during a FORCED VITAL CAPACITY determination. Common abbreviation is MEFV.
Gram-negative bacteria occurring in the lower intestinal tracts of man and other animals. It is the most common species of anaerobic bacteria isolated from human soft tissue infections.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
"AAV5-Factor VIII Gene Transfer in Severe Hemophilia A". N. Engl. J. Med. 377 (26): 2519-2530. doi:10.1056/NEJMoa1708483. hdl: ... However, the Y-chromosome in the male has no gene for factors VIII or IX. If the genes responsible for production of factor ... Factor VIII is used in haemophilia A and factor IX in haemophilia B. Factor replacement can be either isolated from human blood ... Due to differences in changes to the genes involved, people with haemophilia often have some level of active clotting factor. ...
Site-specific gene expression in vivo by direct gene transfer into the arterial wall. Science 1990;249:1285-1288 Nabel EG, Yang ... Recombinant fibroblast growth factor-1 promotes intimal hyperplasia and angiogenesis in arteries in vivo. Nature 1993;362:844- ... Nabel's scientific contributions in cardiovascular gene transfer have developed molecular and cellular techniques, delineated ... Gene therapy for vascular smooth muscle cell proliferation after arterial injury. Science 1994;265:781-784 Capell BC, Olive M, ...
Stochastic models for horizontal gene transfer: taking a random walk through tree space. Genetics, 170:419-431, 2005. Redelings ... Models for estimating Bayes factors with applications to phylogeny and tests of monophyly. Biometrics, In press. Suchard MA. ... Search for a gene by environment interaction: GXE hunt. Genetic Epidemiology 17:S491-S496, 1999. Suchard MA, Yudkin P and ... Evolutionary similarity among genes, Journal of the American Statistical Association 98:653-662, 2003. D'Orsogna MR, Suchard MA ...
Horizontal gene transfer has been demonstrated to occur readily on dry surfaces such as stainless steel, but not on copper and ... The main risk factor for death found by the study was being bedridden, which significantly increased the chance of death. This ... They can increase the expression of chromosome-encoded β-lactamase genes (bla genes) due to regulatory gene and promoter ... Increased horizontal gene transfer (HGT) is observed simultaneously with cell viability on stainless steel surfaces. HGT is one ...
Another factor that reaffirms this fact is that transferred cells had a larger concetration of Annexin V, which is a protein ... Its regulatory factor, IRF3, is essential for activating its gene promoter, and most viruses have proteins to inhibit this ... 3b protein induces an increase of AP-1 dependent gene expression in 3b-transferred cells. Studies showed that the presence of ... This protein is encoded by ORF3b gene and that gene is found in the DNA of SARS-CoV and SARS-CoV-2. The infection caused by ...
For example, content exchange between individuals enables horizontal gene transfer, an important factor in the evolution of ... Neumann, E; Schaefer-Ridder, M; Wang, Y; Hofschneider, PH (1982). "Gene transfer into mouse lyoma cells by electroporation in ... August 2001). "Laboratory-scale evidence for lightning-mediated gene transfer in soil". Appl. Environ. Microbiol. 67 (8): 3440- ... viable daughter cells to be produced would require an extra replication of the intact RNA gene homologous to any RNA gene that ...
Horizontal gene transfer is common in bacteria and between prokaryotes and eukaryotes yet is more rare between eukaryotic ... Lack of viv increased life span in days by a factor of 2.3 compared to the wild type and lack of i by 1.6, however, strain ... gene Gene deletion and allelic replacement in the Wlamentous fungus Podospora anserina Gene deletion and allelic replacement in ... Slot, Jason C.; Rokas, Antonis (2011). "Horizontal Transfer of a Large and Highly Toxic Secondary Metabolic Gene Cluster ...
"Efficient, long-term hepatic gene transfer using clinically relevant HDAd doses by balloon occlusion catheter delivery in ... "Low ascorbate levels are associated with increased hypoxia-inducible factor-1 activity and an aggressive tumor phenotype in ... "Intracellular ascorbate enhances hypoxia-inducible factor (HIF)-hydroxylase activity and preferentially suppresses the HIF-1 ...
The PAI can be acquired by horizontal gene transfer and contains genes for several virulence factors. Two fully sequenced ... the thermostable direct hemolysin gene (tdh) and/or the tdh-related hemolysin gene (trh). Strains possessing one or both of ... Aside from the T3SS, two genes encoding well-characterized virulence proteins are typically found on the PAI, ...
... called factors or genes. Autosomal traits are associated with a single gene on an autosome (non-sex chromosome)-they are called ... Y-linked inheritance occurs when a gene, trait, or disorder is transferred through the Y chromosome. Since Y chromosomes can ... X-linked genes are found on the sex X chromosome. X-linked genes just like autosomal genes have both dominant and recessive ... Genes are the common factor of the qualities of most human-inherited traits. Study of human genetics can answer questions about ...
The therapy transfers a working copy of the Factor VIII gene into patients who lack one. In Phase II clinical trials, 2 of 7 ... Fidanacogene elaparvovec is an adeno-associated viral vector which is designed to transfer a working copy of the Factor IX gene ... Adeno-Associated Virus Mediated Gene Transfer for Hemophilia B Achieves Sustained Mean Factor IX Activity Levels of >30% ... "FDA approves novel gene therapy to treat patients with a rare form of inherited vision loss" (Press release). Food and Drug ...
"AAV5-Factor VIII Gene Transfer in Severe Hemophilia A". New England Journal of Medicine. 16 December 2017. Retrieved 14 ... activity of factor VIII), 45-50% have the same mutation, an inversion within the factor VIII gene that results in total ... Gene therapy[edit]. In December 2017, it was reported that doctors had used a new form of gene therapy to treat haemophilia A.[ ... Factor VIII concentrates[1]. Haemophilia A (or hemophilia A) is a genetic deficiency in clotting factor VIII, which causes ...
CTXφ can transmit cholera toxin genes from one V. cholerae strain to another, one form of horizontal gene transfer. The genes ... Risk factors for these deaths include: third trimester, younger maternal age, severe dehydration, and vomiting Dehydration ... Gene transfer is fairly common amongst bacteria, and recombination of different V. cholerae genes can lead to new virulent ... including essential genes for metabolism, heat-shock proteins, and 16S rRNA genes, which are ribosomal subunit genes used to ...
To identify the genes necessary for reprogramming, the researchers removed one factor at a time from the pool of twenty-four. ... found that they could transfer the four necessary genes with a plasmid. The Yamanaka group successfully reprogrammed mouse ... The original set of reprogramming factors (also dubbed Yamanaka factors) are the transcription factors Oct4 (Pou5f1), Sox2, ... Klf2 and Klf4 were found to be factors capable of generating iPS cells, and related genes Klf1 and Klf5 did as well, although ...
Gene transfer agents are viruslike elements produced by Rhodobacterales which transfer DNA and may be an important factor in ...
"Long-term correction of canine hemophilia B by gene transfer of blood coagulation factor IX mediated by adeno-associated viral ... where she started her career by developing the earliest canine model of hemophilia by cloning the canine Factor IX gene. She ... Her work primarily focused on the use of gene therapy for hemophilia. High received an A.B. in chemistry at Harvard University ... "Factor This! Series: An Interview with Dr. Katherine A. High ·". onthepulseconsultancy.com. 2018-01-19. Retrieved 2019-11-06. ...
Gene transfer is used to express trophic factors or neurotransmitter-producing enzymes in animal models of neurodegenerative ... CNS Gene Therapy Research Group [10] The CNS Gene Therapy Team develop systems for sufficient and sustained expression of ... The medical part of the research focuses on cell transplantation, gene therapy and finding new drugs. New clinical trials where ... Clinical Experimental Therapies in Neurodegenerative and Inflammatory Diseases [13] The projects aim at transferring ...
2009). "Hepatocyte growth factor or vascular endothelial growth factor gene transfer maximizes mesenchymal stem cell-based ... Cells that have the capacity to release soluble factors such as cytokines, chemokines, and growth factors which act in a ... These factors facilitate self-healing of the organ or region by inducing local (stem) cells or attracting cells to migrate ... Van Der Berg DJ, Sharma AK, Bruno E, Hoffman R (1998). "Role of members of the Wnt gene family in human hematopoiesis". Blood. ...
"Reinnervation of motor endplates and increased muscle fiber size after human insulin-like growth factor I gene transfer into ... Other evidence suggests that gene-therapy induced expression of neurotrophic factors within the target muscle itself can also ... Flint PW, Shiotani A, O'Malley BW (March 1999). "IGF-1 gene transfer into denervated rat laryngeal muscle". Archives of ... All these factors contribute to the formation of what is known as a glial scar, which axons cannot grow across. The proximal ...
In addition, the newly recognized factors of symbiogenesis and horizontal gene transfer introduced yet more complexity into ... Indeed, the endosymbiotic theory for the origin of organelles sees a form of horizontal gene transfer as a critical step in the ... These high levels of horizontal gene transfer have led to suggestions that the family tree of today's organisms, the so-called ... Ochman, Howard; Lawrence, Jeffrey G.; Groisman, Eduardo A. (May 18, 2000). "Lateral gene transfer and the nature of bacterial ...
She confirmed the anecdotal assumption that virulence factors (disease-causing genes in pathogens) are disproportionately ... Her research has provided new insights into the evolution of pathogens and the role that horizontal gene transfer and genomic ... Ho Sui, S.J., Fedynak, A., Langille, M.G.I., Hsiao, W.W., and F.S.L. Brinkman (2009). The association of virulence factors with ... and better factor in the important role of societal changes and the environment in shaping microbiomes. Fellow, Royal Society ...
ACLAME (The CLAssification of Mobile genetic Elements) database Horizontal gene transfer Virulence factors Viruses and mobile ... Also called "jumping genes" they can be transferred horizontally between organisms that live in symbiosis. Transposons are ... They can also rearrange genes in the host genome. One of the examples of MGEs in evolutionary context is that virulence factors ... One type of MGEs, namely the Intergrative Conjugative Elements (ICEs) are central to horizontal gene transfer shaping the ...
For example, a model of mouse hemophilia is corrected by expressing wild-type platelet-factor VIII, the gene that is mutated in ... "HIV-2 and SIV Vector Systems." Lentiviral Vector Systems for Gene Transfer. Ed. Gary L. Buchschacher. Georgetown, TX: Eurekah. ... Protease PR encoded by the pro gene (part of pol gene in some viruses). dUTPase DU encoded by the pro gene (part of pol gene in ... February 2007). "Lentivirus-mediated platelet-derived factor VIII gene therapy in murine haemophilia A". J. Thromb. Haemost. 5 ...
Certain bacteria employ mobile genetic elements and horizontal gene transfer. Therefore, strategies to combat certain bacterial ... and intrinsic characteristics of the bacteria called virulence factors. Many virulence factors are so-called effector proteins ... In the specific context of gene for gene systems, often in plants, virulence refers to a pathogen's ability to infect a ... Virus virulence factors allow it to replicate, modify host defenses, and spread within the host, and they are toxic to the host ...
May 2001). "Adenovirus-mediated gene transfer of human platelet-activating factor-acetylhydrolase prevents injury-induced ... T missense in exon 9 of the plasma platelet-activating factor acetylhydrolase gene as an independent risk factor for coronary ... "Entrez Gene: PLA2G7 phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)". Mohler ER, Ballantyne CM ... February 2002). "Association of a G994 -->T missense mutation in the plasma platelet-activating factor acetylhydrolase gene ...
Hyperleptinemia produced by infusion or adenoviral gene transfer decreases blood pressure in rats. Leptin microinjections into ... Factors that acutely affect leptin levels are also factors that influence other markers of inflammation, e.g., testosterone, ... They reviewed a common polymorphism in the leptin gene (A19G; frequency 0.46), three mutations in the leptin receptor gene ( ... and that of a permissive factor in puberty, activator of immune cells, activator of beta islet cells, and growth factor. In ...
Geographic factors in evolution include gene flow and horizontal gene transfer. Spatial game models represent geometry by ... So it is ultimately genes that play out a full contest - selfish genes of strategy. The contesting genes are present in an ... A key factor applicable in these coevolutionary systems is the continuous adaptation of strategy in such arms races. ... Dawkins, Richard (1976). The Selfish Gene. Oxford University Press. pp. 76-78. ISBN 978-0-19-929114-4. Maynard Smith, J. (1982 ...
"An Agrobacterium virulence factor encoded by a Ti plasmid gene or a chromosomal gene is required for T-DNA transfer into plants ... Derbyshire KM, Gray TA (2014). "Distributive Conjugal Transfer: New Insights into Horizontal Gene Transfer and Genetic Exchange ... rhizogenes contain genes that are capable of transferring to plant cells. The expression of these genes effectively transforms ... which together are about 33 kb long and consist of about 40 genes. The tra locus includes the pilin gene and regulatory genes, ...
The phl gene cluster encodes factors for 2,4-DAPG biosynthesis, regulation, export, and degradation. Eight genes, phlHGFACBDE, ... PhlD shows similarity to plant chalcone synthases and has been theorized to originate from horizontal gene transfer. ... This gene plays a key role in gene regulation; when this gene is mutated in the nonfood bacterial strain, it is transformed ... Of these genes, phlD encodes a type III polyketide synthase, representing the key biosynthetic factor for 2,4-DAPG production. ...
The virG protein is a cytoplasmic protein produced from the virG Ti plasmid gene. It is a transcription factor, inducing the ... Products of the other VirB genes are used to transfer the subunits across the plasma membrane. Yeast two-hybrid studies provide ... This strain is just as successful in controlling crown gall as K84 without the caveat of resistance gene transfer. Host, ... Four main genes are involved in this process: chvA, chvB, pscA, and att. The products of the first three genes apparently are ...
As a result, structural proteins, resulting from polypeptide products of gag and gag-pol genes, that are necessary for the HIV ... Integrase strand transfer inhibitors (INSTI)). *Dolutegravir (DTG)°. *Elvitegravir (EVG)°. *Raltegravir (RAL)°. *BI 224436† ...
It has a core set of 1553 genes, plus 154 genes in its virulome, which contribute to virulence and 176 genes that maintain a ... "Pathogenic Properties (Virulence Factors) of Some Common Pathogens" (PDF).. *^ "Clindamycin" (PDF). Davis. 2017. Retrieved ... Natural bacterial transformation involves the transfer of DNA from one bacterium to another through the surrounding medium. ... For instance, the Xisco gene was recently described as a biomarker for PCR-based detection of S. pneumoniae and differentiation ...
a b c d e Eugene Kiely, Medicaid Estate Recovery Program, FactCheck.org, Annenberg Public Policy Center, University of ... The reasons for low use are many, but a lack of dental providers who participate in Medicaid is a key factor.[53][54] Few ... Elders who gift or transfer assets can be caught in the situation of having no money but still not being eligible for Medicaid ... Several political factors influence the cost and eligibility of tax-funded health care, according to a study conducted by ...
3 Gene content and protein synthesis *3.1 Chloroplast genome reduction and gene transfer ... probably with the help of an unknown GDP exchange factor. A domain of Toc159 might be the exchange factor that carry out the ... "Endosymbiotic gene transfer and transcriptional regulation of transferred genes in Paulinella chromatophora". Molecular Biology ... Endosymbiotic gene transfer is how we know about the lost chloroplasts in many chromalveolate lineages. Even if a chloroplast ...
These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ... autologous fat transfer).[161] ... Genes[edit]. Acne appears to be strongly inherited; genetics ... High levels of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) are also associated with worsened acne.[42] Both ... Risk factors for the development of acne, other than genetics, have not been conclusively identified. Possible secondary ...
Potential risk factors[edit]. Temperature and heat exposure[edit]. The temperature and heat levels of the body are directly ... Yamashita, D., Minami, S. B., Kanzaki, S., Ogawa, K., & Miller, J. M. (2008). Bcl-2 genes regulate noise-induced hearing loss. ... This mechanism seeks to reduce the sound energy in the ear by dampening its transfer from eardrum to cochlea. It has been seen ... This factor is particularly interesting due to the fact that a large population of people listen to music while exercising. ...
A gene from rice (Osmyb4), which codes for a transcription factor, that was shown to increase cold and drought tolerance in ... Agrobacterium-mediated genetic engineering techniques were developed in the late 1980s that could successfully transfer genetic ... by adding antisense genes to silence the native gene or by adding extra copies of the native gene.[45][46] ... whereas another used transcription factors from snapdragon (Antirrhinum).[34] When the snapdragon genes were used, the fruits ...
"Eugene Register-Guard. 26 January 1976. Retrieved 27 June 2010.. *^ a b "Explore Our Past:2000 - present". British Airways. ... Passenger load factor (%) 79.1 77.0 78.5 78.5 78.2 79.9 81.3 81.0 81.5 81.2 81.8 ... Passengers wishing to travel internationally with BA either to or from regional UK destinations must now transfer in London.[60 ...
"Entrez Gene: tumor necrosis factor (ligand) superfamily". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView& ... Kolesterilester transfer protein • Klasterin • Faktor stimulacije kolonije • Hemopeksin • Laktoferin • Membranski glikoproteini ... 1999). "BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator.". Science 285 (5425): 260-3. PMID ... nuclear factor-kappaB, and c-Jun NH2-terminal kinase.". J. Biol. Chem. 274 (23): 15978-81. PMID 10347144. doi:10.1074/jbc. ...
The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... L. Brown, Theodore; LeMay, Jr., H. Eugene; Bursten, Bruce E.; Burdge, Julia R. (2003). Chemistry: The Central Science (8th ed ... which are an effect of charge-transfer, electrostatic, and electron correlative contributing phenomena.[161] While analogous ... Therefore, the only factor which affects the atomic radius of the alkali metals is the number of electron shells. Since this ...
... the types of pollen that an ear of corn will accept through expression of certain forms of the Gametophyte Factor 1 gene. Many ... Organic farmers are pursuing the transfer of some of these mechanisms into non-popcorn strains for purposes of preventing ... The popcorn remains free to donate its genes via its own pollen to other types of corn. The effectiveness of this restriction ... thereby preventing the ingression of genes (natural or engineered) from other types of corn. ...
Mallove, Eugene (1991), "Fire from Ice: Searching for the Truth Behind the Cold Fusion Furor", London: Wiley, ISBN 0-471-53139- ... The known rate of the decay process together with the inter-atomic spacing in a metallic crystal makes heat transfer of the 24 ... However, the reduction in separation is not enough by a factor of ten to create the fusion rates claimed in the original ... that possibility is much less probable than that of inadvertent contamination or other explained factors in the measurements ...
... s genes in the offspring. 87.5% of D3's genes would come from S, while D4 would receive 93.75% of their genes from S.[54] ... These studies show that inbreeding depression and ecological factors have an influence on survival.[20] ... The goal of preventing the transfer of deleterious alleles may be achieved by reproductive isolation, sterilization, or, in the ... as the inbreeding first removes many deleterious genes, and permits the expression of genes that allow a population to adapt to ...
2007). "A multi-gene phylogeny of Clavicipitaceae (Ascomycota, Fungi): identification of localized incongruence using a ... Temperature requirements and growth rates are crucial factors that identify O. sinensis from other similar fungi.[6] Climate ... resulting in the naming of a new family Ophiocordycipitaceae and the transfer of several Cordyceps species including C. ...
Genes, factors and transcriptional specificity". Eur J Biochem. 212 (1): 1-11. doi:10.1111/j.1432-1033.1993.tb17626.x. PMID ... The most important RNA genes are transfer RNA (tRNA) and ribosomal RNA (rRNA), both of which are involved in the process of ... Non-coding RNA or "RNA genes". These are a broad class of genes that encode RNA which is not translated into protein. ... However, since the late 1990s, many new RNA genes have been found, and thus RNA genes may play a much more significant role ...
Cruts M, Hendriks L, Van Broeckhoven C (1997). "The presenilin genes: a new gene family involved in Alzheimer disease pathology ... regulation of epidermal growth factor-activated receptor activity. • regulation of resting membrane potential. • regulation of ... Gene ontology. Molecular function. • PDZ domain binding. • cadherin binding. • peptidase activity. • beta-catenin binding. • ... "Entrez Gene: PSEN1 presenilin 1 (Alzheimer disease 3)".. *^ Chan YM, Jan YN (August 1998). "Roles for proteolysis and ...
Some degree of gene flow is normal, and preserves constellations of genes and genotypes.[118][119] An example of this is the ... By discarding taxonomy, human health, and economic factors, this model focused only on ecological factors. The model evaluated ... the pathways of hundreds of marine invasive species and found that shipping was the dominant mechanism for the transfer of ... "Factors influencing red wolf-coyote hybridization in eastern North Carolina, USA (PDF Download Available)". ResearchGate. ...
... is a eukaryotic gene. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of ... RAD51 catalyses strand transfer between a broken sequence and its undamaged homologue to allow re-synthesis of the damaged ... Sigurdsson S, Van Komen S, Petukhova G, Sung P (Nov 2002). "Homologous DNA pairing by human recombination factors Rad51 and ... Many cancers have epigenetic deficiencies in various DNA repair genes (see Frequencies of epimutations in DNA repair genes in ...
Asexual reproduction, horizontal gene transfer. Asexual reproduction, horizontal gene transfer. Sexual and asexual reproduction ... directing the binding of the RNA polymerase to a gene's promoter.[144] However, other archaeal transcription factors are closer ... since most archaeal genes lack introns, although there are many introns in their transfer RNA and ribosomal RNA genes,[146] and ... and the presence of archaea-like genes in certain bacteria, such as Thermotoga maritima, from horizontal gene transfer.[78] The ...
The TATA box has a binding site for the transcription factor of the PG2 gene. This gene produces PG2 serum, which is used as a ... "Single-molecule fluorescence resonance energy transfer shows uniformity in TATA binding protein-induced DNA bending and ... and other transcription factors in some eukaryotic genes. Gene transcription by RNA polymerase II depends on the regulation of ... "Gene. 389 (1): 52-65. doi:10.1016/j.gene.2006.09.029. PMC 1955227. PMID 17123746.. ...
The role of genes and environment in the etiology of PCOS. Endocrine. 2006, 30 (1): 19-26. PMID 17185788. doi:10.1385/ENDO:30:1 ... 風險因子(英语:Risk factor). 肥胖症、運動不足、家族病史[8]. ... Metabolism of triglyceride-rich lipoproteins and lipid transfer to high-density lipoprotein in young obese and normal-weight ... Lin, Eugene C, 编. Imaging in Polycystic Ovary Disease. eMedicine. eMedicine. 20 April 2011 [19 November 2011].. ...
... is an HIV integrase strand transfer inhibitor which blocks the functioning of HIV integrase which is needed for ... who are treatment-naïve or treatment-experienced but have not previously taken other integrase strand transfer inhibitors.[8] ... including those who have been treated with other integrase strand transfer inhibitors. Tivicay is also approved for children ...
The latter is a common alternative to digoxigenin, and the two are used together for labelling two genes in one sample.[3] ... a number of other factors,[specify] however, are considered contributors as well.[citation needed] The nausea usually is ... "Phloem as Capacitor: Radial Transfer of Water into Xylem of Tree Stems Occurs via Symplastic Transport in Ray Parenchyma" ...
Watson JD, Baker TA, Bell SP, Gann A, Levine M, Oosick R (2008). Molecular Biology of the Gene. San Francisco: Pearson/Benjamin ... Transfer RNA (tRNA). *Ribosome-nascent chain complex (RNC). *Post-translational modification (functional groups · peptides · ...
The HADHB gene is located on chromosome 2, with its specific location being 2p23.[5] The gene contains 17 exons. HADHB encodes ... phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in ... 2.3.3: converted into alkyl on transfer. *Citrate synthase. *Decylcitrate synthase. *Citrate (Re)-synthase ... Mutations in this gene, along with mutations in HADHA, result in trifunctional protein deficiency.[5] Mutations in either gene ...
Eugene Paul Wigner United States "for his contributions to the theory of the atomic nucleus and the elementary particles, ... "for his contributions to the theory of electron transfer reactions in chemical systems"[۳۴] ... "for their discoveries of growth factors"[۶۹] ریتا لوی مونتالچینی[۱] Italy ... "for his discovery of آران‌ای سرکوبگر - gene silencing by double-stranded RNA"[۷۹] ...
The primary risk factor for COPD globally is tobacco smoking.[9] Of those who smoke, about 20% will get COPD,[44] and of those ... and worsening of gas transfer. This can also lead to insufficient ventilation, and eventually low blood oxygen levels.[5] Low ... "Genes and chronic obstructive pulmonary disease". The Medical Clinics of North America. 96 (4): 699-711. doi:10.1016/j.mcna. ... The most common cause of COPD is tobacco smoking, with a smaller number of cases due to factors such as air pollution and ...
Integrase strand transfer inhibitors (INSTI)). *Dolutegravir (DTG)°. *Elvitegravir (EVG)°. *Raltegravir (RAL)°. *BI 224436† ...
Gene therapy[edit]. Gammaretroviral and lentiviral vectors for gene therapy have been developed that mediate stable genetic ... a short repeated sequence at each end of the genome used during the reverse transcription to ensure correct end-to-end transfer ... "Cell-to-cell transmission of retroviruses: Innate immunity and interferon-induced restriction factors". Virology. 411 (2): 251 ... For example, the gag gene is translated into molecules of the capsid protein, the pol gene is translated into molecules of ...
The initial long-range positional information of the maternal factors, which are transcription factors (or are factors which ... 1980). Genes of the BX-C regulate pattern formation in part of the thorax and in the abdomen, whereas different genes of the ... Transfer of a single nucleus at a specific stage of development, to an enucleated unfertilized egg, provided an opportunity to ... Maternal-effect genes - subject to Maternal (cytoplasmic) inheritance *Egg-polarity genes establish the Anteroposterior axis.[ ...
AAV5-factor VIII gene transfer in severe hemophilia A. N. Engl. J. Med. 377, 2519-2530 (2017). ... A macrophage colony-stimulating factor receptor-green fluorescent protein transgene is expressed throughout the mononuclear ...
The Evans Musculoskeletal Gene Therapy Research Lab at Mayo Clinic studies gene therapy for knee osteoarthritis, bone defects, ... Improved tendon healing by administration of growth factors. *Enhanced repair of defects in cartilage by gene transfer ... The vector transfers a gene encoding an anti-arthritic protein, the interleukin-1 receptor antagonist (IL-1Ra), to cells within ... Healing of critical size segmental defects in bone by gene transfer. *Healing of critical size segmental defects in bone using ...
Gene transfer agents are viruslike elements produced by Rhodobacterales which transfer DNA and may be an important factor in ... Gene transfer agents are viruslike elements produced by Rhodobacterales which transfer DNA and may be an important factor in ...
The results indicated that IL-1Ra or vIL-10 gene transfer significantly inhibited IL-1β and tumor necrosis factor (TNF) ... The results indicated that IL-1Ra or vIL-10 gene transfer significantly inhibited IL-1β and tumor necrosis factor (TNF) ... The results indicated that IL-1Ra or vIL-10 gene transfer significantly inhibited IL-1β and tumor necrosis factor (TNF) ... The results indicated that IL-1Ra or vIL-10 gene transfer significantly inhibited IL-1β and tumor necrosis factor (TNF) ...
1. Genetics - Genes are units of heredity transferred from one generation to another. They are the inherited traits that ... A: Several factors contribute to the formation of drug addiction and dependence, but the cause of addiction boils down to the ... In addition to peer influence, family relationships have also shown as a contributing factor to the formation of addiction. ...
These resistant bacteria can then replicate and/or transfer resistance genes to other bacteria to pass on resistance, growing ... All of these factors cause "selection pressure" in bacteria which pressure the bacteria to adapt to their surroundings and ... If antibiotic use on the farm is a contributing factor to human resistance, the answer is not as easy as simply removing the ...
... are food crops that whose genes have been altered in some ways in order to improve them. Alternatively called genetically ... Gene coding for human growth factor has also been successfully transferred into rats to increase their sizes. These ... was the issue of allergies suffered by certain individuals against the products of the gene being transferred to other crops. ... For instance, genes which made fishes in the arctic regions of the world survive the extreme cold have been successfully ...
Theyre also starting a new trial transferring a gene that will help brain cells produce GDNF, a growth factor that has the ... these scientists are studying the safety and effectiveness of using modified viral vectors to transfer genes into the brains of ... in a novel approach to modify the course of Huntingtons disease by the infusion of a viral vector linked to a gene that can ...
Hwang, D. H., Jeong, S. R. and Kim, B. G. (2011). Gene transfer mediated by stem cell grafts to treat CNS injury. Expert Opin ... Hepatocyte growth factor reduces astrocytic scar formation and promotes axonal growth beyond glial scars after spinal cord ... Differential and cooperative actions of Olig1 and Olig2 transcription factors on immature proliferating cells after contusive ... grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor- ...
Horizontal gene transfer gratis norske pornofilmer milf bergen involves the non-sexual interspecific transmission of genetic ... Flytende spell styrker og kompakt hår fiber gir slapt hår Polynomials factoring polynomials - quadratic forms:. Utnyttelse som ...
The gene transfer of omega-3 fatty acid desaturase"; World Rev Nutr Diet; 92:23‐36; 2003; https://pubmed.ncbi.nlm.nih.gov/ ... There are multiple factors that go into an efficient metabolism, though one key component is AMP-activated protein kinase (AMPK ... Dehydrozingerone: Gingers Forgotten Anti-Inflammatory Weight Loss Factor. Posted on June 10, 2020 by Mike Roberto and CJ ... Zhang Q. et al; "Circulating Oxidized Low-Density Lipoprotein is a Strong Risk Factor for the Early Stage of Coronary Heart ...
Clinical trial for A Gene Transfer Study for Hemophilia A. ... the safety and efficacy of the factor VIII gene transfer ... A Gene Transfer Study for Hemophilia A Hemophilia A Clinical Trial. Official title:. Gene Transfer, Dose-Finding Safety, ... to deliver the human factor VIII (hFVIII) gene into liver cells so that they can produce factor VIII protein. ; ... It is caused by a mutation or deletion in the gene that is responsible for producing blood-clotting factor VIII protein. ...
Selective regulation of cytokine induction by adenoviral gene transfer of IkappaBalpha into human macrophages: ... lipopolysaccharide-induced, but not zymosan-induced, proinflammatory cytokines are inhibited, but IL-10 is nuclear factor- ... metalloproteinase 1 and matrix metalloproteinase 3 in rheumatoid arthritis patients following anti-tumour necrosis factor-alpha ...
... inducers and inhibitors of P5CS gene, expression pattern of P5CS under differential conditions in studied plant species are ... Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat. ... Characterization of the gene for Δ1-pyrroline-5-carboxylate synthetase and correlation between the expression of the gene and ... Gene regulation. Action of leucine zippers. Nature, 341: 24-25. [2] Abrahám, E., Rigó, G., Székely, G., Nagy, R., Koncz, C. and ...
These remaining genes encode factors that increase the synthesis, or generation, of the acid in ways that are still largely ... The first step in their process was to transfer the genes of the artemisinic acid pathways core set of enzymes into the ... Their method involves transferring its metabolic pathway - the series of biochemical steps involved in its production - from A ... A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop. eLife ...
Collectively, these results suggest that something can be transferred from one strain to another (such as the netB gene) ... Factors. Samples were also taken from birds that were challenged with C. perfringens to artificially cause NE. All the strains ... It is not clear if antibiotic use in poultry is a contributing factor, but it is clear that antibiotics have an effect on ... The implication here is that netB may only contribute to NE and that other factors (such as management practices) are involved ...
Genetic and environmental factors influence LDL size, cholesteryl ester transfer protein (CETP) being one of the candidate ... About LDL related genes:. A Clinical study by group of reserchers from UK and Australia reported linkage of the CETP gene to ... Genes affect the way we use excess fat in our body that can lead to heart disease. The story above has more data that I could ... genes. CETP mediates the transfer of cholesteryl ester from HDL to apolipoprotein (apo) B-containing lipoproteins in exchange ...
... horizontal gene transfer plants is likely enhanced by with... Incidence of horizontal gene transfer plants is likely enhanced ... And environmental factors by commenting below a ) Eichornia crassipes because cleistogamous flowers never open at all from ... E.g. The incidence of horizontal gene transfer plants is likely enhanced by associations with. The amounts of sexual and ... The incidence of horizontal gene transfer plants is likely enhanced by associations with are given four flowers namely maize ...
A remote control for targeted gene transfer into cells. Posted on 05 July, 2021 ... Age is the factor that explains most of the genetic mutations associated with the appearance and development of different ... Researchers are set to commence the first Phase 1 human trial of a COVID-19 gene-based DNA vaccine in Australia, delivered via ... which means only cells that are illuminated with red light take up the desired genes. [ + ] ...
Protection from endotoxemia by adenoviral-mediated gene transfer of human bactericidal/permeability-increasing protein Academic ... Vascular endothelial growth factor is an important determinant of sepsis morbidity and mortality Academic Article ... Adenoviral vector-mediated interleukin-10 expression in vivo: intramuscular gene transfer inhibits cytokine responses in ... Effects of coagulation factor XIII on intestinal functional capillary density, leukocyte adherence and mesenteric plasma ...
... in a process called Horizontal Gene Transfer (HGT). This was the primary factor in very early evolution, not random mutation. ... But horizontal gene transfer still continued after this transition, and continues to this day. (Ibid., pp. 182-84) Its hard to ... Because of the high level of HGT [horizontal gene transfer], evolution at this stage would in essence be communal, not ... horizontal gene transfer, matter, mechanism, metaphors, mutation, Physics, random chance, vitalism / 3 Comments ...
... are annotating the genes associated with chemosensation and vision, immunity, reproductive physiology, horizontal transfer ... to compare the genomes of five tsetse species and determine the genetic factors responsible for their peculiar nutrition and ... The BecA-ILRI Hub Advisory Panel chair Eugene Terry has been recognized by the Modernizing African Food System Consortium (MAFS ... The decoding of the tsetse flys genome to reveal the genes responsible for its peculiar reproductive and feeding habits opened ...
... or transfer RNA genes. "Only sequences flanked by RNA hairpin structures similar to miRNA precursors were retained, and reads ... These miRNAs were also up- or downregulated when the miRNA biogenesis factors Dicer, Drosha, DGCR8, or Ago2 were silenced. ... he told Gene Silencing News. "This led us to think that the actual number of human microRNA genes is much bigger than what is ... In Science this week: pair of mutations in one gene uncovered in brothers with short stature and immunodeficiency, and more. ...
This dangerous phenomenon, called "horizontal gene transfer," allows rapid movement of traits like antibiotic resistance among ... Although these factors could help explain why a political decision to isolate China allowed two strains to move through the ...
30 They are presently used in approximately a fifth of all clinical trials requiring gene transfer delivery. 31 In addition to ... Identification of coordination factors affecting 2016-1-1 · σ-retroviral vectors were the first viral vectors used to provide ... 30 They are presently used in approximately a fifth of all clinical trials requiring gene transfer delivery. 31 In addition to ... 30 They are presently used in approximately a fifth of all clinical trials requiring gene transfer delivery. 31 In addition to ...
These elements enable a horizontal gene transfer, whereby a bacterium can acquire new genes and thus characteristics. ... They primarily support the rapid mutation of bacteria into various factors. Similar to the other genetic material, the plasmids ... Other Eubacteria may have pili, small protrusions on the outside of the cell that is used to adhere to surfaces and transfer ... can be transferred to daughter cells during replication. They are the DNA structure commonly used in research because they are ...
... mechanism of horizontal gene transfer has been widely linked to the acquisition of antibiotic resistance and virulence factors ... mechanism of horizontal gene transfer has been widely linked to the acquisition of antibiotic resistance and virulence factors ...
Some of the factors influencing T-DNA transfer to #C. glauca$ explants were studied. Optimal transformation rates were obtained ... TRANSFERT DE GENE; ETUDE COMPARATIVE; CAFEIER; GENOME; INTROGRESSION; FLUX DE GENES; MARQUEUR MOLECULAIRE; GENE DE RESISTANCE; ... Agrobacterium tumefaciens gene transfer to Casuarina glauca : a tropical nitrogen-fixing tree IRD. ... Palavras-chave: AMELIORATION DES PLANTES; RIZ; TRANSFERT DE GENE; TAUX; EFFICACITE; SELECTION; GENE; CHIMERE; TRANSFORMATION ...
  • HepG2 cells were infected with 1,000 vp/cell of AdKO1 in the presence of 1 IU/ml of FX and 2.5% sera from patients previously screened for anti-Ad NAbs and stained for expression of the β-Gal reporter gene 48 h postinfection. (nih.gov)
  • We have investigated the role of 5'-flanking DNA sequences in regulating the expression of the human TF gene in human umbilical vein endothelial cells (HUVEC). (nih.gov)
  • Using these conditions, varying lengths of TF 5'-flanking sequences coupled to the CAT reporter gene were tested in transient expression studies. (nih.gov)
  • A domain from -382 to -111bp, which contains two AP-1 consensus elements, promoted high levels of gene expression. (nih.gov)
  • While the genetics of F transfer have been described extensively, the mechanism of conjugation has proved elusive, in large part because of the low levels of expression of the pilus and the numerous envelope components essential for F plasmid transfer. (asm.org)
  • Background: Data from earlier hemophilia B (HB) AAV-mediated liver gene transfer trials demonstrated a dose-dependent, capsid-specific immune response that may result in clearance of transduced hepatocytes and loss of transgene expression (Manno et al. (confex.com)
  • Prior work incorporated the use of steroids to abort this immune response and maintain factor IX (FIX) expression (Nathwani et al 2014). (confex.com)
  • We tested several engineered AAV capsids for factor IX (FIX) expression for the treatment of hemophilia B by hepatic gene transfer. (biomedcentral.com)
  • The glial cell was chosen as the target cell for gene transfer, and the expression of VEGF was s tudied in vitro. (iospress.com)
  • Expression of biologically active human insulin-like growth factor-I following intramuscular injection of a formulated plasmid in rats. (semanticscholar.org)
  • Fibroblast growth factor-2 gene transfer can stimulate hepatocyte growth factor expression irrespective of hypoxia-mediated downregulation in ischemic limbs. (pubfacts.com)
  • In the early expression, FGF-2 directly stimulated HGF mRNA without the requirement of new protein synthesis, whereas sustained induction of HGF in the later phase was partly mediated by platelet-derived growth factor-AA. (pubfacts.com)
  • Histological and immunohistochemical techniques were used to determine growth factor, collagen expression, and dermal and epidermal structure. (elsevier.com)
  • MO-7e cells infected with a retrovirus encoding the human MDR1 cDNA showed integration, transcription, and expression of the transfered MDR1 gene. (elsevier.com)
  • The expression of the MDR1 gene product was stable over a 6-month period in vitro without selection in colchicine. (elsevier.com)
  • These results show that expression of the MDR1 gene in human hemetopoietic progenitor cells can confer functional drug resistance in an in vivo model. (elsevier.com)
  • SF gene expression was detected in conditioned medium of 9L-SF but not in control 9L-neo-transfected cell lines, by reverse transcriptase-PCR, immunoblot, ELISA, and scatter activity assays. (elsevier.com)
  • We characterized the pattern of gene expression after systemic administration of a recombinant vaccinia virus encoding a reporter gene in a murine in vivo model of s.c. human melanoma. (elsevier.com)
  • Gene expression in tumor tissue was increased 100-fold as compared with normal tissue, providing evidence for tumor-selective gene delivery. (elsevier.com)
  • Combined transcription and genome data from multiple tissues in hundreds of human donors reveal links between genotype and gene expression across the body. (the-scientist.com)
  • We also explored the mechanisms behind this phenomenon and demonstrate that nanoalumina is able to induce oxidative stress, damage bacterial cell membranes, enhance the expression of mating pair formation genes and DNA transfer and replication genes, and depress the expression of global regulatory genes that regulate the conjugative transfer of RP4. (pnas.org)
  • A recombined adenovirus carrying PEDF gene (Ad-PEDF) was prepared, and its expression by infected cells and in treated animals was confirmed with Western blotting and ELISA, respectively. (biomedcentral.com)
  • The effect of lentiviral expression of truncated soluble vascular endothelial growth factor decoy receptor (tsFlk-1) in MSC on growth of Raji cells was determined, both in vitro and in vivo. (elsevier.com)
  • Using retroviral-mediated gene transfer and viral integration patterns, Steel factor (SCF) in combination with IL-6 appears to increase the survival and self-renewal of reconstituting hematopoietic stem cells and proves useful in effecting expression of foreign genes in transplant recipients. (ashpublications.org)
  • Conclusions: Stimulation of hepatocellular mitosis and upregulation of Ram-1 expression by HGF and T3 augment retrovirus-mediated gene transfer into hepatocytes. (elsevier.com)
  • Gene transfer with expression, as measured by RT-PCR for hIGF-I mRNA, occurred in 81.3% of injected animals. (elsevier.com)
  • Emphasis is placed on biotechnological applications of bacteria such as the cloning of bacterial genes, expression of recombinant proteins for therapeutic and industrial uses and development of biological control agents. (edu.au)
  • Objectives We set out to investigate the ability of cardiotropic adeno-associated viral vector (AAV2.9 = recombinant adeno-associated virus [rAAV]) to induce prolonged expression of vascular endothelial growth factor (VEGF)-A and platelet-derived growth factor (PDGF)-B in a rabbit hindlimb ischemia model and a pig model of hibernating myocardium. (onlinejacc.org)
  • Glial cell line-derived neurotrophic factor (GDNF) strongly modulates the viability and phenotypic expression of dopamine ventral mesencephalic neurons. (jneurosci.org)
  • The first WRKY transcription factor, (SPF1), was identified in 1994 for its involvement in sucrose regulation of gene expression in sweet potato (Ipomoea batatas). (wikipedia.org)
  • This was the first of many studies identifying WRKY transcription factors in regulating gene expression for plant defense. (wikipedia.org)
  • They described newly discovered but as yet ill-defined DNA binding proteins that played potential roles in the regulation of gene expression by sucrose (SPF1) or during germination (ABF1 and ABF2). (wikipedia.org)
  • Miami Winter Symposia, Volume 16: From Gene to Protein: Information Transfer in Normal and Abnormal Cells presents the expression and processing of genetic information at the levels of both proteins and nucleic acids. (elsevier.com)
  • This text then examines the molecular mechanism by which steroid hormones regulate specific gene expression. (elsevier.com)
  • Since then, Tuddenham has not only been trying to fix the gene but also to improve its expression. (scientificamerican.com)
  • 2.2.4 Expression of other genes from Thermus genomic libraries. (barnesandnoble.com)
  • In this study, gene transfer was used to induce IL-10 transgene expression in murine lungs for treatment of endotoxin-induced lung inflammation. (cdc.gov)
  • Expression of the second set of flagellar genes seems to be positively regulated under anaerobic growth conditions. (asm.org)
  • Expression of the flagellar genes is regulated in a hierarchical pattern which results from coordination of flagellar gene expression at the transcriptional or posttranscriptional level with one or more structural checkpoints in flagellum biogenesis ( 31 ). (asm.org)
  • Time-rap video system was established to analyze the differentiation of ES cells and the expression pattern of GFP-tagged genes. (nii.ac.jp)
  • Ischemia or hypoxia may increase vascular permeability, enhance the release of chemoattractive factors, and promote the expression of adhesion proteins, which may facilitate the homing process. (ahajournals.org)
  • Gene transfer was assessed using semiquantitative reverse transcription-polymerase chain reaction analysis, enzyme-linked immunosorbent assay for human VEGF and β-galactosidase expression in injured rats treated with Matrigel plus Ad.CMV.LACZ, Matrigel plus Ad.CMV.VEGF 165 , and untreated injured rats. (thejns.org)
  • Decker JT, Hobson EC, Zhang Y, Shin S, Thomas AL, Jeruss JS, Arnold KB, Shea LD (2017) Systems analysis of dynamic transcription factor activity identifies targets for treatment in Olaparib resistant cancer cells. (sc.edu)
  • Hepatocyte Growth Factor (HGF) has potent angiogenic and anti-apoptotic activities, and this study evaluated the functional and biochemical effects of HGF gene transfer in a rat model of postinfarction heart failure. (ahajournals.org)
  • Conclusions- Hepatocyte Growth Factor gene transfer following a large myocardial infarction results in significantly preserved myocardial function and geometry, and is associated with significant angiogenesis and a reduction in apoptosis. (ahajournals.org)
  • Hepatocyte Growth Factor (HGF) is a heterodimeric pluripotent growth factor with an 80-kDa apparent molecular weight that has been shown to have potent angiogenic actions on various cells types. (ahajournals.org)
  • Hepatocyte growth factor (HGF) is a potent angiogenic polypeptide that stimulates angiogenesis. (pubfacts.com)
  • Scatter factor (SF), also known as hepatocyte growth factor, is angiogenic in systemic tissues, and SF titers correlate with the malignancy and metastatic phenotype of certain systemic cancers. (elsevier.com)
  • We hypothesized that induction of hepatocellular proliferation by combined administration of two hepatocellular mitogens, hepatocyte growth factor (HGF) and triiodothyronine (T3), should permit rMoMuLV-mediated gene transfer into liver without invasive approaches. (elsevier.com)
  • The purpose of this study was to determine whether AAV-mediated intraocular gene transfer of pigment epithelium-derived factor (PEDF) inhibits the development of choroidal neovascularization (CNV) in a murine model. (nih.gov)
  • In the present study, we report that angiogenic growth factors, including HGF, were upregulated in a murine model of critical limb ischemia in vivo, a finding that was in conflict with previous in vitro data. (pubfacts.com)
  • We have examined the effect of prestimulation of bone marrow with various cytokines, including the product of the Steel gene, Steel factor or stem cell factor (SCF) (the ligand for the c-kit receptor) on the efficiency of retroviral transduction of the human adenosine deaminase (hADA) cDNA into murine HSC. (ashpublications.org)
  • AND-34, a novel GDP exchange factor, is expressed constitutively at significant levels in murine splenic B cells, but not in murine splenic T cells or thymocytes. (jimmunol.org)
  • New generation of plasmid backbones devoid of antibiotic resistance marker for gene therapy trials. (semanticscholar.org)
  • The extent to which nanomaterials are able to cause an increase in antibiotic resistance by the regulation of the conjugative transfer of antibiotic-resistance genes in bacteria, especially across genera, is still unknown. (pnas.org)
  • The spread of antibiotic-resistance genes is due to the selective pressures caused by increases in the use and misuse of antibiotics in medicine and animal feedstuffs ( 4 ). (pnas.org)
  • Aquatic environments and water treatment processes are able to affect the efficiency of antibiotic-resistance gene transfer ( 9 ). (pnas.org)
  • However, it is unknown whether or how nanomaterials affect antibiotic-resistance gene transfer between bacteria, especially across genera. (pnas.org)
  • Resistance transfer factor (shortened as R-factor or RTF) is an old name for a plasmid that codes for antibiotic resistance. (wikipedia.org)
  • Often, R-factors code for more than one antibiotic resistance factor: genes that encode resistance to unrelated antibiotics may be carried on a single R-factor, sometimes up to 8 different resistances. (wikipedia.org)
  • Many R-factors can pass from one bacterium to another through bacterial conjugation and are a common means by which antibiotic resistance spreads between bacterial species, genera and even families. (wikipedia.org)
  • Since many R-factors contain F-plasmids, antibiotic resistance can be easily spread among a population of bacteria. (wikipedia.org)
  • Also, R-factors can be taken up by "DNA pumps" in their membranes via transformation, or less commonly through viral mediated transduction, or via bacteriophage, although conjugation is the most common means of antibiotic resistance spread. (wikipedia.org)
  • AMR results from pathogenic strains of bacteria adapting to antimicrobial-containing environments through mutations or through horizontal gene transfer (HGT) of genetic material containing resistance genes. (k-state.edu)
  • Adeno-associated virus (AAV) gene therapy vectors have shown the best outcomes in human clinical studies for the treatment of genetic diseases such as hemophilia. (biomedcentral.com)
  • Gene Transfer is the insertion of genetic material into a cell. (news-medical.net)
  • SCID-X1 is a genetic disorder of blood cells caused by DNA changes in a gene that is required for the normal development of the human immune system. (clinicaltrials.gov)
  • 1 They offer, for example, the possibility to select embryos with the greatest potential of being born healthy (eg, preimplantation genetic testing (PGT) i ) and, perhaps, one day to correct genes responsible for heritable diseases in the embryo (eg, human germline genome modification (HGGM) ii ). (bmj.com)
  • This pore allows the transfer of genetic material, such as a plasmid. (wikipedia.org)
  • Conjugation allows two bacteria, not necessarily from the same species, to transfer genetic material one way. (wikipedia.org)
  • Haemophilia A (or hemophilia A ) is a genetic deficiency in clotting factor VIII , which causes increased bleeding and usually affects males. (wikipedia.org)
  • The new method enables rapid screening for t ransfer of genetic characteristics from an introduced bacterial population and may be an important screen for gene transfer from environmentally released genetically engineered bacteria into native bacterial populations. (highbeam.com)
  • Hemophilia A is the most common X-linked genetic disease and the second most common factor deficiency after von Willebrand disease (vWD). (medscape.com)
  • As a result of an inherited genetic mutation, people with haemophilia B lack a protein called factor IX that is crucial for forming blood clots. (scientificamerican.com)
  • The rate at which the factor VIII gene produces its protein is affected in part by the placement of the triplets of DNA bases-codons-that dictate where translation of the genetic material into protein should start and stop. (scientificamerican.com)
  • Genetic engineering assumes additional significance, because of the possibility of introducing a desired gene in a single step for precision breeding of forest trees. (springer.com)
  • 2.2.6 Genetic transfer and plasmids in Thermus. (barnesandnoble.com)
  • 2.2.12 Other genes and genetic systems in Bacillus stearothermophilus. (barnesandnoble.com)
  • Explore the normal functions of human genes and the health implications of genetic changes. (medlineplus.gov)
  • When such vectors are delivered intravenously, hepatocyte transduction is mediated by the hexon-coagulation factor X (FX) interaction. (nih.gov)
  • Replication-deficient (E1, E3 deleted) adenoviral vectors containing the rat HGF transgene driven by the human muscle creatine kinase (MCK) promoter were made and obtained from the Institute of Human Gene Therapy, University of Pennsylvania (Adeno-HGF). (ahajournals.org)
  • Several alternative strategies for capsid modification improve the in vivo performance of AAV vectors in hepatic gene transfer for correction of hemophilia. (biomedcentral.com)
  • Adeno-associated viral (AAV) vectors represent some of the most powerful tools for in vivo gene transfer to a variety of organs. (biomedcentral.com)
  • AAV vectors have also been in clinical development for hepatic gene transfer over the past decade, primarily to express coagulation factors in hepatocytes for the treatment of the X-linked bleeding disorder hemophilia. (biomedcentral.com)
  • Researchers led by a team at Massachusetts Eye and Ear have identified a novel cellular entry factor for adeno-associated virus vector (AAV) types--the most commonly used viral vectors for in vivo gene therapy. (news-medical.net)
  • Clinical trials in hemophilia using adeno-associated virus (AAV) vectors to transfer functional factor IX (FIX) have reported increases in FIX activity to functional levels, reduced bleed frequency, and a lessening or abrogation of the need for costly FIX replacement. (cdc.gov)
  • However, in an unrelated study conducted in France, of pediatric subjects administered integrating retrovirus vectors for X-linked severe combined immune deficiency (X-SCID), several leukemias developed years after transfer of the γc common chain gene. (clinicaltrials.gov)
  • It highlights the potential of using viral vectors for a gene therapy approach to treat, or perhaps prevent, Alzheimer's disease. (prohealth.com)
  • Prior to relocating to Cardiff, I was studying adenoviral vectors for translational applications in cardiovascular disease, where my research had pinpointed key virus interactions with host blood coagulation factors (most notably Factor X) that dictate the tropism and toxicity of intravenously administered viral vectors. (cardiff.ac.uk)
  • My desire to work on viral vectors for gene and cell therapy applications resulting in my relocating to Glasgow University from 2005-2013, where I made seminal contributions to how Adenoviral (Ad) based vectors interact with host cells and proteins, and how these interactions dictate viral tropism and toxicity. (cardiff.ac.uk)
  • Our vector core is a full service facility with more than a decade experience in the production of viral-based platforms for gene delivery including lentiviral vectors, adeno-associated vectors (AAV), adenovirus and Herpes Simplex Virus (HSV). (sc.edu)
  • Vascular endothelial growth factor (VEGF) is most promising in therapeutic angiogenesis for ischemic vascular disease. (iospress.com)
  • Direct intramuscular gene transfer of naked DNA encoding vascular endothelial growth factor augments collateral development and tissue perfusion. (semanticscholar.org)
  • It is not clear whether treatment by vascular endothelial growth factor (VEGF) gene transfer can improve myocardial ischemia through a proangiogenesis mechanism and is effective against coronary artery disease (CAD). (cdc.gov)
  • One hallmark of melanoma progression is angiogenesis, which is induced by angiogenic factors released by tumor cells and characterized as the formation of a new vascular network from pre-existing blood vessels. (biomedcentral.com)
  • Background - This phase 1/2 study investigated the safety of percutaneous catheter-based gene transfer of naked plasmid DNA encoding for vascular endothelial growth factor 2 (phVEGF2) to left ventricular (LV) myocardium in a prospective, randomized, double-blind, placebo-controlled, dose-escalating study of inoperable patients with class III or IV angina. (elsevier.com)
  • To overcome the deficiency of both blood flow and growth factors, the authors added exogenous vascular endothelial growth factor (VEGF 165 ) to the transected spinal cord either as recombinant protein alone or combined with an adenovirus coding for VEGF 165 . (thejns.org)
  • The efficiency of retroviral-mediated gene transfer into hematopoietic stem cells (HSC) is dependent on the survival and self-renewal of HSC in vitro during retroviral infection. (ashpublications.org)
  • In a Phase I human gene therapy trial, eight immunocompetent prostate cancer (PCA) patients were treated with autologous, GM-CSF-secreting, irradiated tumor vaccines prepared from ex vivo retroviral transduction of surgically harvested cells. (aacrjournals.org)
  • To develop a rapid preclinical in vivo model to study gene transfer into human hematopoietic progenitor cells, MO-7e cells (CD-34 + , c-kit + ) were infected with multidrug resistance (MDR1)-containing retroviruses and then transplanted into nonobese diabetic severe combined immunodeficient mice (NOD SCID). (elsevier.com)
  • Supernatants of vvEMAP-transfected tumor cells mediated the induction of tissue factor in endothelial cells. (elsevier.com)
  • Optogenetics is a recently developed technique that can control cellular functions by illuminating lights to the cells in which light-sensitive proteins are expressed by gene transfer. (news-medical.net)
  • We show that activation of the stress response within tumor cells during cell death, using an engineered form of the heat shock transcription factor, leads to an immunogenic death. (duke.edu)
  • The recombinant PEDF adenovirus is able to transfer the PEDF gene to infected cells and successfully produce secretory PEDF protein, which exhibits potent inhibitory effects on HUVEC proliferation. (biomedcentral.com)
  • Background: Efficient gene transfer to bone marrow derived mesenchymal stem cells (MSC) would provide an important opportunity to express potent anticancer agents in the tumour microenvironment because of their contribution to the tumour stroma. (elsevier.com)
  • The strain lacking both acvB and virJ had an impaired ability to transfer T‐DNA into plant cells, suggesting that the factor encoded by virJ or acvB is required for T‐DNA transfer from A. tumefaciens to plant cells. (elsevier.com)
  • Bone marrow cells were prestimulated for 48 hours with hematopoietic growth factors, then cocultivated with the packaging cell line producing the ZipPGK-ADA simplified retrovirus for an additional 48 hours with continued growth factor exposure. (ashpublications.org)
  • Such pretreatment may also be useful in the application of retroviral transfer methods to human cells. (ashpublications.org)
  • Gene delivery approaches offer strong tools to optimize the use of human bone marrow-derived mesenchymal stem cells (hMSCs) for cartilage repair purposes. (biomedcentral.com)
  • This method involves transferring a normal copy of the common gamma chain gene into the participant's bone marrow stem cells. (clinicaltrials.gov)
  • These cells will undergo vector transduction with the lentiviral vector that contains a normal copy of the γc gene gene (CL20-i4-EF1α-hγc-OPT) and then the transduced cells will be reinfused back into the patient. (clinicaltrials.gov)
  • Participants will undergo infusion with autologous CD34+ bone marrow cells transduced with a lentiviral vector that contains a normal copy of the human γc gene. (clinicaltrials.gov)
  • Be familiar with the mechanisms by which bacteria can exchange fragments of DNA, the mechanisms by which self-transmissible DNA fragments maintain their presence in bacterial cells and how they may mediate their own transfer to other bacterial cells. (edu.au)
  • Some of these cells colocalized with GDNF-ir, indicating that an alteration in phenotype may occur by the direct actions of this trophic factor. (jneurosci.org)
  • Specially trained laboratory technicians then use a method known as adenoviral mediated gene transfer, which adds a new gene to the cancer cells. (knowcancer.com)
  • This gene causes the cells to make GM-CSF, a powerful hormone that stimulates the immune system. (knowcancer.com)
  • DNA with a functional factor IX gene was bundled into the molecular wrapper of a virus-known as AAV8-then shuttled into liver cells, where factor IX is normally made. (scientificamerican.com)
  • One good candidate to test for neuroregenerative ability is the class 4 POU domain transcription factor, Brn3b, which is selectively expressed in retinal ganglion cells. (arvojournals.org)
  • The purpose of this study was to determine if adeno-associated virus (AAV) mediated overexpression of transcription factor Brn3b in retinal ganglion cells could promote axonal regeneration after optic nerve injury in glaucomatous rats. (arvojournals.org)
  • However, low precursor frequencies of HPV16 specific T cells in patients and healthy donors hampers routine isolation of these cells for adoptive transfer purposes. (biomedcentral.com)
  • An alternative to generate HPV specific CD4+ and CD8+ T cells is TCR gene transfer. (biomedcentral.com)
  • Our data indicate that TCR transfer is feasible as an alternative strategy to generate human HPV16 specific CD4+ T helper cells for the treatment of patients suffering from cervical cancer and other HPV16 induced malignancies. (biomedcentral.com)
  • Adoptive transfer of HPV specific T cells could be an attractive strategy to treat patients suffering from HPV induced malignancies. (biomedcentral.com)
  • 1) Transient overexpression of transcription factor Ets1 promoted apoptosis of endothelial cells (Ecs). (nii.ac.jp)
  • 6) GFP-tagged genes were stably introduced into mouse embryonic stem (ES) cells. (nii.ac.jp)
  • Melanoma cells produce growth factors for autocrine growth control and for stimulating fibroblasts and endothelial cells in the tumor stroma. (aacrjournals.org)
  • We studied this feedback from fibroblasts to melanoma cells by overexpressing insulin-like growth factor 1 (IGF-1) with an adenoviral vector. (aacrjournals.org)
  • These studies demonstrate that fibroblast-derived growth factors from the tumor environment can provide the malignant cells with a positive feedback through multiple mechanisms but that this stimulation is required only for cells from early and not late stages of tumor progression. (aacrjournals.org)
  • Practical Implications of Factor IX Gene Transfer for Individuals with Hemophilia B: A Clinical Perspective. (cdc.gov)
  • Insulin-like growth factor-I E peptides: implications for aging skeletal muscle. (biomedsearch.com)
  • Numerous growth factors have been utilized in attempts to induce therapeutic angiogenesis by gene transfer or as recombinant proteins, with mixed results in clinical trials to date. (ahajournals.org)
  • An animal model of stress-induced regional myocardial ischemia was used to test the hypothesis that intracoronary delivery of an adenovirus encoding fibroblast growth factor Type 5 (FGF5) would result in improved blood flow, function and neovascularization in the ischemic region of the heart. (brillonline.com)
  • Human insulinlike growth factor 1 gene transfer into paralyzed rat larynx: single vs multiple injection. (semanticscholar.org)
  • OBJECTIVE To compare the biological effects of single vs multiple treatment of rat denervated laryngeal muscle with human insulinlike growth factor 1 (hIGF1) gene therapy. (semanticscholar.org)
  • Reinnervation of motor endplates and increased muscle fiber size after human insulin-like growth factor I gene transfer into the paralyzed larynx. (semanticscholar.org)
  • Mice deficient in basic fibroblast growth factor-2 (FGF-2) showed reduced induction of HGF protein in ischemic muscles, and overexpression of FGF-2 via gene transfer stimulated endogenous HGF, irrespective of the presence of ischemia. (pubfacts.com)
  • Fibroblast growth factor 1 (FGF1) is an angiogenic factor known to play a role in the growth of arteries. (semanticscholar.org)
  • Naked plasmid DNA encoding fibroblast growth factor type 1 for the treatment of end-stage unreconstructible lower extremity ischemia: preliminary results of a phase I trial. (semanticscholar.org)
  • Keratinocyte growth factor (KGF) stimulates epithelial cell differentiation and proliferation, which are of major importance for wound healing. (uni-regensburg.de)
  • however, interaction between different growth factors during dermal and epidermal regeneration are still not entirely defined. (elsevier.com)
  • In the present study, we thought to determine the interaction between keratinocyte growth factor (KGF) administered as liposomal cDNA with other dermal and epidermal growth factors and collagen synthesis in an acute wound. (elsevier.com)
  • Results: KGF cDNA increased insulin-like growth factor-I (IGF-I), insulin-like growth factor binding protein-3 (IGFBP-3), and fibroblast growth factor (FGF), decreased transforming growth factor-beta (TGF-β), while it had no effect on platelet-derived growth factor (PDGF) levels in the wound. (elsevier.com)
  • Exogenous insulin like growth factor-1 (IGF-1) is known to be neuroprotective in animal models with brain insults, while it can also cause hyperexcitability in rodents. (frontiersin.org)
  • insulin growth factor-1 (IGF-1), and its receptors are found to increase in associated brain lesions, implying a role of IGF-1 in the pathological response to these brain insults. (frontiersin.org)
  • Insulin like growth factor-1 is demonstrated to be neuroprotective after brain trauma or stroke. (frontiersin.org)
  • Gene-modified MSC expressing a gene of therapeutic potential can moderate growth of haematological malignancies. (elsevier.com)
  • In the present study, we examined the potential benefits of sustained overexpression of the mitogenic and pro-anabolic insulin-like growth factor I (IGF-I) via gene transfer upon the biological activities of human MSCs (hMSCs). (biomedcentral.com)
  • Using this model, the human insulin-like growth factor I (hIGF-I) gene was introduced into paralyzed rat laryngeal muscle to assess the benefit of sustained local hIGF-I production. (elsevier.com)
  • In skeletal muscle there is good evidence to suggest that locally produced insulin-like growth factor-1 (IGF-I), rather than circulating IGF-I, is important in regard to muscle mass maintenance, repair and hypertrophy. (biomedsearch.com)
  • p130Cas and HEF1 are a structurally related pair of such docking molecules that localize to the focal adhesion complex, assembling members of adhesion and growth factor-related signaling cascades ( 10 , 11 ). (jimmunol.org)
  • The table transfectants exhibited the prolongation of cell-division capability and were suggested to produce a novel autocrine growth factor. (nii.ac.jp)
  • This phenomenon is thought to be related to either secondary ischemia or deficiency of growth factors. (thejns.org)
  • Because most growth factors are rapidly inactivated in the extracellular environment, the authors used an adenovirus coding for VEGF 165 to maintain its activity for several days. (thejns.org)
  • Activated stromal fibroblasts can in turn secrete growth factors that support tumor growth. (aacrjournals.org)
  • This independence from exogenous growth factors is apparently a result of the increased constitutive production of growth factors. (aacrjournals.org)
  • Phosphorylation of Akt is also induced by IGF-1 in several tumors (21 , 22 , 23 , 24) , suggesting a role for this growth factor as an antiapoptotic survival factor. (aacrjournals.org)
  • Receptor tyrosine kinases (RTKs) are a family of cell surface receptors responding to growth factors, hormones, and cytokines to mediate a diverse set of fundamental cellular and metabolic signaling pathways. (stanford.edu)
  • Two introns interrupt the region encoding the transit peptide and six are found within the region encoding the mature subunit, four of which are in identical or similar positions relative to genes for cytosolic GAPDH of eukaryotic organisms. (pnas.org)
  • The author presents basic information on eukaryotic genes and follows this with details of the changes that occur in their structure and function during aging. (worldcat.org)
  • Angiogenesis was significantly enhanced in HGF treated animals as measured by both Von Willebrand's Factor immunohistochemical staining and a microsphere assay. (ahajournals.org)
  • As a potent inhibitor of tumor angiogenesis, pigment epithelium-derived factor (PEDF) has recently been studied and used as an anticancer agent in several tumor models. (biomedcentral.com)
  • Background Gene therapy to induce angiogenesis and arteriogenesis has produced mixed results. (onlinejacc.org)
  • Molecular mechanism of angiogenesis transcription factors and their therapeutic relevance'Pharmacol.Ther. (nii.ac.jp)
  • Staphylococcus and Pasteurellaceae ), suggesting that Pasteurellaceae can acquire antimicrobial resistance (AMR) genes from closely contacting Staphylococcus spp. (mdpi.com)
  • NAbs associate with the capsid, preventing transduction of the target cell ( 19 , 20 ) and limiting its efficacy for gene transfer in vivo ( 8 ), while serving to prime a robust immunological response against the vector ( 4 ). (asm.org)
  • For example, high vector doses are often used for hepatic gene transfer, and cytotoxic T lymphocyte responses against viral capsid may occur. (biomedcentral.com)
  • Enhancing the performance of AAV gene transfer could potentially result in therapy at lower vector doses, which should also reduce capsid antigen presentation. (biomedcentral.com)
  • EXPERIMENTAL METHODS OR DESIGN: A muscle-specific nonviral vector containing the alpha-actin promoter and hIGF1 gene formulated with polyvinyl polymers was injected into denervated adult rat thyroarytenoid muscle. (semanticscholar.org)
  • Gene therapy investigators can greatly benefit from the resources and services provided by the National Gene Vector Biorepository, housed at the Indiana University School of Medicine. (news-medical.net)
  • Several subjects enrolled in a multi-site, gene transfer clinical study to evaluate the intrahepatic administration of AAV2-hFIX16 vector for the treatment of severe hemophilia B between 2001 and 2009. (clinicaltrials.gov)
  • A prior clinical gene transfer study of intrahepatic adeno-associated virus (AAV) vector administration of the factor IX gene (AAV2-hFIX16) to adult subjects with severe hemophilia B enrolled several subjects from 2001 through 2004. (clinicaltrials.gov)
  • However, the current findings support the concept of using this type of vector as an effective tool to treat articular cartilage defects via gene- and stem cell-based procedures. (biomedcentral.com)
  • A muscle-specific nonviral vector containing the α-actin promoter and hIGF-I gene was used in formulation with a polyvinyl-based delivery system and injected into paralyzed adult rat laryngeal muscle. (elsevier.com)
  • In this study, thus, GDNF gene therapy using an adenoviral vector (rAd-GDNF) was examined in rats following SCI induced by dropping the NYU weight-drop impactor from a height of 25 mm onto spinal segment T9-T10. (ncku.edu.tw)
  • Rhabditis is an excellent laboratory model to study internal gene transfer because (a) these microbial worms are bacterial feeders which vector viable bacteria (Chang et al. (highbeam.com)
  • Control rats received Matrigel alone or Matrigel plus an adenoviral vector containing the LACZ gene (Ad.CMV.LACZ). (thejns.org)
  • The vector core provides service for researchers both intramural and extramural to USC, interested in utilizing viral-based method of gene delivery. (sc.edu)
  • Conjugation machinery offers an efficient method for acquisition of AMR and virulence genes, which may be responsible for propelling the evolution of pathogenic bacteria. (k-state.edu)
  • This dissertation explores the factors, specifically catecholamines and antimicrobials that influence the conjugation frequencies of enteric bacteria including Salmonella, E. coli and Enterococcus. (k-state.edu)
  • Researchers have been able to identify and track the exchange of genes among bacteria that allow them to become resistant to drugs, according to a new study published today in eLife. (news-medical.net)
  • Transfer of DNA sequences between species regardless of their evolutionary distance is very common in bacteria, but evidence that horizontal gene transfer (HGT) also occurs in multicellular organisms has been accumulating in the past few years. (mdpi.com)
  • Conjugative transfer between closely related strains or species of bacteria is an important method for the horizontal transfer of multidrug-resistance genes. (pnas.org)
  • Also important is the presence of increasing amounts of these substances in the environment by horizontal transfer between bacteria rather than by the sequential modification of gene function by the accumulation of point mutations ( 5 ). (pnas.org)
  • Horizontal transfer often occurs between closely related strains or species of bacteria but occurs at a very low frequency across genera ( 6 ), although the medical importance of the latter is greater. (pnas.org)
  • Studies have demonstrated that water and wastewater, which are pools for antibiotic-resistant bacteria and -resistance genes, appear to contribute significantly to the acquisition of resistance against antibiotics by bacteria by horizontal DNA transfer ( 7 , 8 ). (pnas.org)
  • 4.4.1 Factors influencing the selection of bacteria for mineral-leaching processes. (barnesandnoble.com)
  • Potential for promoting recurrent laryngeal nerve regeneration by remote delivery of viral gene therapy. (semanticscholar.org)
  • We hypothesized that delivering KGF as a non-viral liposomal cDNA gene complex is a new approach that would effectively enhance dermal and epidermal regeneration. (uni-regensburg.de)
  • Liposomes containing the KGF cDNA gene constructs were effective in improving epidermal and dermal regeneration. (uni-regensburg.de)
  • Biotechnological interventions for in vitro regeneration, mass micropropagation and gene transfer methods in forest tree species have been practised with success, especially in the last decade. (springer.com)
  • Administration of recombinant glial cell line-derived neurotrophic factor (GDNF) has previously been reported to rescue neurons following severe SCI, resulting in improved hindlimb locomotion in rats. (ncku.edu.tw)
  • Twenty-eight days after a single injection, gene transfer efficiency, muscle fiber size, motor endplate length, and nerve-to-motor endplate contact were evaluated. (elsevier.com)
  • Here we show that nanomaterials in water can significantly promote the horizontal conjugative transfer of multidrug-resistance genes mediated by the RP4, RK2, and pCF10 plasmids. (pnas.org)
  • Based on the above data, we hypothesized that nanomaterials that are present in water may promote the horizontal transfer of multidrug-resistance genes across bacterial genera by acting on cell membranes and/or regulating genes involved in plasmid transfer. (pnas.org)
  • My interest in gene and cell therapy began whilst studying for my undergraduate degree in Genetics (University of Sheffield), which inspired me to pursue a PhD in this field. (cardiff.ac.uk)
  • Adenoviruses, particularly those based on adenovirus serotype 5 (Ad5), are used for a variety of gene delivery applications both in vitro and in vivo, since they efficiently transduce a wide range of cell types. (asm.org)
  • In 1994 and 1995, the first two reports of WRKY transcription factors appeared. (wikipedia.org)
  • Arokiaraj P, Jones H, Cheong KF, Coomber S, Charlwood BV (1994) Gene insertion on in to Hevea brasiliensis . (springer.com)
  • AAV-mediated gene transfer of pigment epithelium-derived factor inhibits choroidal neovascularization. (nih.gov)
  • Effect of neutralizing sera on factor x-mediated adenovirus serotype 5 gene transfer. (nih.gov)
  • Erratum: Prolonged and effective blockade of tumor necrosis factor activity through adenovirus-mediated gene transfer (Proc. (elsevier.com)
  • To evaluate the efficacy of intraspinal injection of recombinant adenovirus into the injured spinal cord, we observed green fluorescent protein (GFP) gene transfer in the contused spinal cord. (ncku.edu.tw)
  • In experimental rats, either human recombinant VEGF 165 or a combination of this factor and a replication-defective adenovirus coding for VEGF 165 (Ad.CMV.VEGF 165 ) was applied at the lesion site. (thejns.org)
  • A strong gene transfer in the spinal cord tissue of adenovirus-treated rats was found from Day 3 to Day 10 postinjury, confirming infection. (thejns.org)
  • This paper aimed to study VEGF gene therapy for the treatment of cerebral ischemia. (iospress.com)
  • Gene therapy may be a promising approach for treatment of brain ischemia. (elsevier.com)
  • In this study, we examined the effect of postischemic gene transfer of midkine, a heparin-binding neurotrophic factor, using a focal brain ischemia model with the photothrombotic occlusion method. (elsevier.com)
  • Thus, gene transfer of midkine to the ischemic brain may be effective in the treatment of brain ischemia. (elsevier.com)
  • Iida, M. / Postischemic gene transfer of midkine, a neurotrophic factor, protects against focal brain ischemia . (elsevier.com)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. (uniprot.org)
  • Pivotal to hepatic transduction is the interaction between the Ad5 hexon protein and the coagulation factor X (FX) ( 7 , 24 , 26 ), which serves to "bridge" the Ad5-FX complex to alternative receptors, which are expressed abundantly within the liver. (asm.org)
  • A molecule that naturally degrades a protein linked to Alzheimer's disease appears to reduce the levels of that protein by nearly 50 percent when delivered by gene therapy, researchers at the Salk Institute and University of California, San Diego have found in collaboration with researchers at the University of Kentucky. (prohealth.com)
  • The study indicates that gene therapy may provide an effective way to treat Alzheimer's by producing - in the body - a molecule that reduces naturally the accumulation of the protein beta-amyloid. (prohealth.com)
  • Robert Marr, working under Inder Verma at the Salk Institute in collaboration with the laboratories of Fred H. Gage at the Salk Institute and Eliezer Masliah at UCSD, used a modified version of the HIV virus to transfer a gene called neprilysin to neurons of transgenic mice producing human beta-amyloid, which lowered levels of the harmful protein to half that found in untreated animals. (prohealth.com)
  • WRKY transcription factors are denoted by a 60-70 amino acid WRKY protein domain composed of a conserved WRKYGQK motif and a zinc-finger region. (wikipedia.org)
  • About 5-10% of people with haemophilia A are affected because they make a dysfunctional version of the factor VIII protein , while the remainder are affected because they produce factor VIII in insufficient amounts (quantitative deficiency). (wikipedia.org)
  • 1% activity of factor VIII), 45-50% have the same mutation, an inversion within the factor VIII gene that results in total elimination of protein production. (wikipedia.org)
  • From Kelly Rae Chi at Nature: n 2004, oncologist Gideon Rechavi at Tel Aviv University in Israel and his colleagues compared all the human genomic DNA sequences then available with their corresponding messenger RNAs - the molecules that carry the information needed to make a protein from a gene. (uncommondescent.com)
  • The section they delete encodes a specific region of the protein-called the B-domain-that ensures efficient secretion of factor VIII. (scientificamerican.com)
  • The start and stop codons in the DNA sequence of a normal mouse or human factor VIII gene, did not promote vigorous protein production. (scientificamerican.com)
  • Tumor necrosis factor α (TNF-α) is a proinflammatory cytokine with potent experimental antitumor activity. (elsevier.com)
  • To achieve tumor selective gene delivery, we constructed a recombinant vaccinia virus encoding the human EMAP-II gene (vvEMAP). (elsevier.com)
  • Induction of cell stress through gene transfer of an engineered heat shock transcription factor enhances tumor immunogenicity. (duke.edu)
  • Administration of the endotoxin caused a marked increase in lung inflammation as indicated by increased tumor necrosis factor (TNF)-alpha release and neutrophil count. (cdc.gov)
  • Mice transgenic for IGF-1 under a keratin 1 promoter show marked thickening of the dermis and hypodermis, and the skin is more prone to transformation in initiation/promotion protocols (17) , suggesting that the constitutive production of IGF-1 is a critical factor in tumor development and progression. (aacrjournals.org)
  • These results suggest that the basal transcription of the human TF gene in HUVEC is mediated through at least two negative regulatory elements upstream of the proximal promoter domain. (nih.gov)
  • Most WRKY transcription factors bind to the W-box promoter element that has a consensus sequence of TTGACC/T. Individual WRKY proteins do appear in the human protozoan parasite Giardia lamblia and slime mold Dictyostelium discoideum. (wikipedia.org)
  • This study was designed to investigate the functional, angiogenic and anti-apoptotic effects of adenoviral-mediated gene transfer of HGF in a rat model of postinfarction heart failure. (ahajournals.org)
  • The purpose of this study is to determine if a new method, called lentiviral gene transfer, can be used to treat SCID-X1. (clinicaltrials.gov)
  • KGF gene transfer to acute wounds may represent a new therapeutic strategy to enhance wound healing. (uni-regensburg.de)
  • This approach using gene therapy to sensitize primarily unresponsive tumors toward TNF-α may enhance the usefulness of TNF-α in clinical treatment strategies by increasing the window for the therapeutic application of the cytokine, thus reducing the dose necessary for antitumor responses and subsequently reduce toxicity. (elsevier.com)
  • One therapeutic conundrum is the development of inhibitor antibodies against factor VIII due to frequent infusions. (wikipedia.org)
  • Collectively, the results suggest that adenoviral gene transfer of GDNF can preserve neuronal fibers and promote hindlimb locomotor recovery from spinal cord contusion. (ncku.edu.tw)
  • Gene therapy for severe hemophilia is on the cusp of entering clinical practice. (cdc.gov)
  • Therefore, this clinical perspective article will review the evidence supporting gene therapy in this field, examine ways to open a dialogue about gene therapy with patients in the clinic setting, and present a case of a participant in a recent clinical trial of gene therapy for hemophilia. (cdc.gov)
  • Examining a clinical case in hemophilia B more closely, gene transfer decreased annualized bleeds from six (unknown or spontaneous) bleeds before treatment to three (spontaneous) bleeds after treatment alongside a 55% reduction in FIX replacement. (cdc.gov)
  • After the gene transfer, the participant considered his hemophilia to be "cured," which emphasizes the need to manage patient expectations, particularly regarding activity levels and bleed risk in the immediate post-treatment period. (cdc.gov)
  • Gene therapy for hemophilia has the potential to transform the lives of affected individuals and is likely to create a new class of hemophilia patient who has shifted from a severe to a mild phenotype. (cdc.gov)
  • What are alternative treatment options for hemophilia A with factor VIII (FVIII) inhibitors? (medscape.com)
  • Factor VIII products and inhibitor development in severe hemophilia A. N Engl J Med . (medscape.com)
  • A Randomized Trial of Factor VIII and Neutralizing Antibodies in Hemophilia A. N Engl J Med . (medscape.com)
  • Hemophilia A and parahemophilia: deficiencies of coagulation factors VIII and V. Scriver CR, Beaudet AL, Sly WS, Valle D, eds. (medscape.com)
  • WRKY transcription factors (pronounced 'worky') are proteins that bind DNA. (wikipedia.org)
  • They are transcription factors that regulate many processes in plants and algae (Viridiplantae), such as the responses to biotic and abiotic stresses, senescence, seed dormancy and seed germination and some developmental processes but also contribute to secondary metabolism. (wikipedia.org)
  • Based on the amino acid sequence WRKY transcription factors are classified into three major categories, group I, group II, and group III. (wikipedia.org)
  • In 1996 three parsley (Petroselinum crispum) WRKY transcription factors (WRKY1, WRKY2, and WRKY3) were identified for their role in regulation of the PATHOGENESIS RELATED1 gene. (wikipedia.org)
  • Since then WRKY transcription factors have been extensively investigated for stress tolerance in many agronomic and horticulture crops. (wikipedia.org)
  • The capacity of conjugative DNA transfer is a property of self-transmissible plasmids and conjugative transposons, which will mobilize other plasmids and DNA sequences that include a compatible oriT locus. (asm.org)
  • This review will concentrate on the genes required for bacterial conjugation that are encoded within the transfer region (or regions) of conjugative plasmids. (asm.org)
  • This dissertation defines the role of outside factors in conjugative gene transfer, and may provide future insight into better control of AMR. (k-state.edu)
  • Nanoalumina can promote the conjugative transfer of the RP4 plasmid from Escherichia coli to Salmonella spp. (pnas.org)
  • Brain-derived neurotrophic factor (BDNF) is involved in many aspects of the formation of functional neuronal networks. (jneurosci.org)
  • We deduce from this that eubacteria and chloroplasts contained GT-AG introns until relatively recently and that the duplication event leading to the genes encoding GAPB and GAPA and their respective transit peptides occurred in the chloroplast progenitor prior to the successive transfer and functional reintegration of these genes into the nuclear environment. (pnas.org)
  • Rather than infusing functional clotting factors, the therapy aims to get the body to create its own. (scientificamerican.com)
  • The amino acid sequence of virJ is similar to a region of a previously characterized chromosomal gene, acvB, required for virulence. (elsevier.com)
  • This includes the concepts of virulence and virulence factors, opportunistic pathogens, and predisposing factors to disease. (edu.au)
  • Our researchers plan to use Transomic's CRISPR genome-editing tools to knockout specific genes of interest. (sc.edu)
  • The present result demonstrated the feasibility of choosing the glial cell as the target cell for VEGF gene transfer, and found the rationale for the cerebral VEGF gene therapy. (iospress.com)
  • We aimed to perform a systematic review and meta-analysis of randomized controlled trials (RCTs) that compared VEGF gene therapy and standard treatments in CAD. (cdc.gov)
  • This study supports a role for neprilysin in the regulation of beta-amyloid," said Verma, a Salk professor who is internationally recognized for his research on gene therapy. (prohealth.com)
  • Bacterial conjugation results in the transfer of DNA of either plasmid or chromosomal origin between microorganisms. (asm.org)
  • However, there is limited clinical experience in this area given that gene transfer is a relatively recent technology. (cdc.gov)
  • As the US FDA has established guidelines for the long-term follow-up (LTFU) of subjects receiving investigational gene therapy products, this protocol seeks to characterize the clinical outcome and the type and seriousness of adverse events following the AAV gene transfer. (clinicaltrials.gov)
  • Adverse event reporting will focus on the development of oncologic, hematologic, neurologic and auto-immune events following gene transfer as specified in the November 2006 FDA Center for Biologics Evaluation and Research (CBER) guidance document entitled, 'Gene Therapy Clinical Trials - Observing Subjects for Delayed Adverse Events. (clinicaltrials.gov)
  • This research should provide information for developing a clinical strategy for GDNF gene therapy. (ncku.edu.tw)
  • The factor VIII/von Willebrand factor complex: basic and clinical issues. (medscape.com)
  • In 2011, after 37 years of pain and joint degeneration caused by internal bleeding, Martin signed up for a clinical trial of a gene-therapy treatment at the Royal Free Hospital in London, hoping that it would provide some relief. (scientificamerican.com)
  • Tissue factor (TF) is a cellular receptor and cofactor for factor VII/VIIa which initiates the blood coagulation cascade. (nih.gov)
  • The viral genes present in these sequences, sometimes of hymenopteran origin, have been co-opted by lepidopteran species to confer some protection against pathogens. (mdpi.com)
  • A blastp clustering search at 100% aa identity cut-off and sharing at least one gene between plasmids, followed by a multilevel community network analysis revealed that a surprisingly large number of the plasmids were connected by one largest connected component (LCC), with dozens of community sub-groupings. (mdpi.com)
  • Materials and methods: Rats received an acute wound and were divided into two groups to receive weekly subcutaneous injections of liposomes plus the Lac-Z gene (0.22 μg, vehicle), or liposomes plus the KGF cDNA (2.2 μg) and Lac-Z gene (0.22 μg). (elsevier.com)
  • In addition, we applied the viral transfer of astrocytic IGF-1 to rodents treated with KA and assessed the associated molecular marker and behavioral outcomes in these rodents. (frontiersin.org)
  • This book reviews the molecular biology of the gene in relation to aging. (worldcat.org)
  • Genes and Aging is for all students and researchers interested in the molecular biology of aging. (worldcat.org)
  • Deficiency in coagulation factor VIII is the cause of haemophilia A. (wikipedia.org)
  • These develop as the body recognises the infused factor VIII as foreign, as the body does not produce its own copy . (wikipedia.org)
  • In these individuals, activated factor VII , a precursor to factor VIII in the coagulation cascade , can be infused as a treatment for haemorrhage in individuals with haemophilia and antibodies against replacement factor VIII. (wikipedia.org)
  • However, mild haemophilia A is known to occur in heterozygous females due to X-inactivation , so it is recommended that levels of factor VIII and IX be measured in all known or potential carriers prior to surgery and in the event of clinically significant bleeding. (wikipedia.org)
  • Verbruggen B, Novakova I, Wessels H, Boezeman J, van den Berg M, Mauser-Bunschoten E. The Nijmegen modification of the Bethesda assay for factor VIII:C inhibitors: improved specificity and reliability. (medscape.com)
  • Detection of all anti-factor VIII antibodies in haemophilia A patients by the Bethesda assay and a more sensitive immunoprecipitation assay. (medscape.com)
  • Spreafico M, Peyvandi F. Combined Factor V and Factor VIII Deficiency. (medscape.com)
  • The inherited, combined deficiency of factors V and VIII is a rare but recognized cause of a bleeding disorder. (medscape.com)
  • The viral vehicle AAV8 is ideal for treating haemophilia B, but it works less well for haemophilia A. This is because the DNA encoding the clotting factor that is missing in the latter-factor VIII-is about six times larger than for factor IX, so it doesn't fit into AAV8. (scientificamerican.com)
  • To make it fit, researchers often cut 4,500 base pairs out of the factor VIII gene sequence. (scientificamerican.com)
  • But in a 2010 study of haemophiliac mice, these B-domain-modified treatments did not increase the level of factor VIII expressed in the blood. (scientificamerican.com)
  • In 2015, Tuddenham and his team hope to lead trials to test safety and efficacy of their optimized factor VIII gene therapy for people with haemophilia A. The number of people in the trials is likely to be between 10 and 20, but even if the factor is expressed effectively in humans, there are still hurdles to overcome. (scientificamerican.com)
  • Be familiar with the ways in which bacterial pathogens can be transmitted to humans, and the factors that influence transmission of pathogens and the occurrence of infectious diseases. (edu.au)
  • These data suggest that both T-cell and B-cell immune responses to human PCA can be generated by treatment with irradiated, GM-CSF gene-transduced PCA vaccines. (aacrjournals.org)
  • A placebo-controlled trial of recombinant human ciliary neurotrophic (rhCNTF) factor in amyotrophic lateral sclerosis. (semanticscholar.org)
  • Human FGF-1 gene transfer promotes the formation of collateral vessels and arterioles in ischemic muscles of hypercholesterolemic hamsters. (semanticscholar.org)
  • We hypothesized that transfer of the EMAP-H gene into established TNF-resistant human melanomas would render these tumors sensitive to subsequent systemic TNF-α treatment. (elsevier.com)
  • Finally, human melanomas in nude mice were sensitized in vivo by transferring the EMAP-II gene using vvEMAP. (elsevier.com)
  • Human Gene Therapy , 9 (14), 2039-2047. (elsevier.com)
  • A study identifies behavioral, psychological, and social factors that could predict human mortality. (pnas.org)
  • 8) Human TERT gene and SV40 T antigen gene were introduced into human umbilical vein ECs (HUVECS). (nii.ac.jp)
  • Where possible, data will be obtained for up to 15 years following hepatic AAV2-hFIX16 gene transfer. (clinicaltrials.gov)