Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Libraries, MedicalMolecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Libraries: Collections of systematically acquired and organized information resources, and usually providing assistance to users. (ERIC Thesaurus, http://www.eric.ed.gov/ accessed 2/1/2008)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Methanobrevibacter: A genus of gram-positive, anaerobic, cocci to short rod-shaped ARCHAEA, in the family METHANOBACTERIACEAE, order METHANOBACTERIALES. They are found in the GASTROINTESTINAL TRACT or other anoxic environments.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Combinatorial Chemistry Techniques: A technology, in which sets of reactions for solution or solid-phase synthesis, is used to create molecular libraries for analysis of compounds on a large scale.Genes, Bacterial: The functional hereditary units of BACTERIA.Library Services: Services offered to the library user. They include reference and circulation.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Libraries, Hospital: Information centers primarily serving the needs of hospital medical staff and sometimes also providing patient education and other services.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Biodiversity: The variety of all native living organisms and their various forms and interrelationships.Genomic Library: A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).Directed Molecular Evolution: The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Biota: The spectrum of different living organisms inhabiting a particular region, habitat, or biotope.Proteobacteria: A phylum of bacteria consisting of the purple bacteria and their relatives which form a branch of the eubacterial tree. This group of predominantly gram-negative bacteria is classified based on homology of equivalent nucleotide sequences of 16S ribosomal RNA or by hybridization of ribosomal RNA or DNA with 16S and 23S ribosomal RNA.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Cosmids: Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.National Library of Medicine (U.S.): An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.Genes: A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Library Surveys: Collection and analysis of data pertaining to operations of a particular library, library system, or group of independent libraries, with recommendations for improvement and/or ordered plans for further development.Sequence Homology, Nucleic Acid: The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.Library Administration: Planning, organizing, staffing, direction, and control of libraries.RNA, Archaeal: Ribonucleic acid in archaea having regulatory and catalytic roles as well as involvement in protein synthesis.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Archaea: One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.DNA, Archaeal: Deoxyribonucleic acid that makes up the genetic material of archaea.Small Molecule Libraries: Large collections of small molecules (molecular weight about 600 or less), of similar or diverse nature which are used for high-throughput screening analysis of the gene function, protein interaction, cellular processing, biochemical pathways, or other chemical interactions.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.Library Science: Study of the principles and practices of library administration and services.Libraries, Digital: Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.Libraries, NursingGenetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Library AssociationsRNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Catalogs, LibraryDNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Library Collection Development: Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.Geologic Sediments: A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)Bacterial Proteins: Proteins found in any species of bacterium.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Ecosystem: A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Repetitive Sequences, Nucleic Acid: Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Library Technical Services: Acquisition, organization, and preparation of library materials for use, including selection, weeding, cataloging, classification, and preservation.Cluster Analysis: A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.Library Automation: The use of automatic machines or processing devices in libraries. The automation may be applied to library administrative activities, office procedures, and delivery of library services to users.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)DNA, Fungal: Deoxyribonucleic acid that makes up the genetic material of fungi.Library Materials: Print and non-print materials collected, processed, and stored by libraries. They comprise books, periodicals, pamphlets, reports, microforms, maps, manuscripts, motion pictures, and all other forms of audiovisual records. (Harrod, The Librarians' Glossary, 4th ed, p497)Codon: A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Recombinant Proteins: Proteins prepared by recombinant DNA technology.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Genetic Variation: Genotypic differences observed among individuals in a population.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Interlibrary LoansLibraries, DentalLibrary Schools: Educational institutions for individuals specializing in the field of library science or information.Facility Design and Construction: Architecture, exterior and interior design, and construction of facilities other than hospitals, e.g., dental schools, medical schools, ambulatory care clinics, and specified units of health care facilities. The concept also includes architecture, design, and construction of specialized contained, controlled, or closed research environments including those of space labs and stations.Librarians: Specialists in the management of a library or the services rendered by a library, bringing professional skills to administration, organization of material and personnel, interpretation of bibliothecal rules, the development and maintenance of the library's collection, and the provision of information services.Chromosomes, Artificial, Bacterial: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.MEDLARS: A computerized biomedical bibliographic storage and retrieval system operated by the NATIONAL LIBRARY OF MEDICINE. MEDLARS stands for Medical Literature Analysis and Retrieval System, which was first introduced in 1964 and evolved into an online system in 1971 called MEDLINE (MEDLARS Online). As other online databases were developed, MEDLARS became the name of the entire NLM information system while MEDLINE became the name of the premier database. MEDLARS was used to produce the former printed Cumulated Index Medicus, and the printed monthly Index Medicus, until that publication ceased in December 2004.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Cataloging: Activities performed in the preparation of bibliographic records for CATALOGS. It is carried out according to a set of rules and contains information enabling the user to know what is available and where items can be found.High-Throughput Screening Assays: Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.BooksArchitecture as Topic: The art and science of designing buildings and structures. More generally, it is the design of the total built environment, including town planning, urban design, and landscape architecture.Bacteriophage M13: Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Online Systems: Systems where the input data enter the computer directly from the point of origin (usually a terminal or workstation) and/or in which output data are transmitted directly to that terminal point of origin. (Sippl, Computer Dictionary, 4th ed)Information Services: Organized services to provide information on any questions an individual might have using databases and other sources. (From Random House Unabridged Dictionary, 2d ed)Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Book CollectingBook SelectionProtein Engineering: Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Interior Design and Furnishings: The planning of the furnishings and decorations of an architectural interior.Bibliography as Topic: Discussion of lists of works, documents or other publications, usually with some relationship between them, e.g., by a given author, on a given subject, or published in a given place, and differing from a catalog in that its contents are restricted to holdings of a single collection, library, or group of libraries. (from The ALA Glossary of Library and Information Science, 1983)Two-Hybrid System Techniques: Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Bacteriophages: Viruses whose hosts are bacterial cells.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Contig Mapping: Overlapping of cloned or sequenced DNA to construct a continuous region of a gene, chromosome or genome.Information Systems: Integrated set of files, procedures, and equipment for the storage, manipulation, and retrieval of information.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Drug Evaluation, Preclinical: Preclinical testing of drugs in experimental animals or in vitro for their biological and toxic effects and potential clinical applications.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Single-Chain Antibodies: A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.Organization and Administration: The planning and managing of programs, services, and resources.Minicomputers: Small computers that lack the speed, memory capacity, and instructional capability of the full-size computer but usually retain its programmable flexibility. They are larger, faster, and more flexible, powerful, and expensive than microcomputers.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Reference Books, Medical: Books in the field of medicine intended primarily for consultation.Sequence Tagged Sites: Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.Cell Surface Display Techniques: Techniques utilizing cells that express RECOMBINANT FUSION PROTEINS engineered to translocate through the CELL MEMBRANE and remain attached to the outside of the cell.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Societies: Organizations composed of members with common interests and whose professions may be similar.High-Throughput Nucleotide Sequencing: Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Drug Design: The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Mutagenesis, Insertional: Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.Immunoglobulin Fragments: Partial immunoglobulin molecules resulting from selective cleavage by proteolytic enzymes or generated through PROTEIN ENGINEERING techniques.DNA Shuffling: The use of DNA recombination (RECOMBINATION, GENETIC) to prepare a large gene library of novel, chimeric genes from a population of randomly fragmented DNA from related gene sequences.CD-ROM: An optical disk storage system for computers on which data can be read or from which data can be retrieved but not entered or modified. A CD-ROM unit is almost identical to the compact disk playback device for home use.Planning Techniques: Procedures, strategies, and theories of planning.Databases, Bibliographic: Extensive collections, reputedly complete, of references and citations to books, articles, publications, etc., generally on a single subject or specialized subject area. Databases can operate through automated files, libraries, or computer disks. The concept should be differentiated from DATABASES, FACTUAL which is used for collections of data and facts apart from bibliographic references to them.MicrofilmingSubstrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The Genexpress IMAGE knowledge base of the human brain transcriptome: a prototype integrated resource for functional and computational genomics. (1/9359)Expression profiles of 5058 human gene transcripts represented by an array of 7451 clones from the first IMAGE Consortium cDNA library from infant brain have been collected by semiquantitative hybridization of the array with complex probes derived by reverse transcription of mRNA from brain and five other human tissues. Twenty-one percent of the clones corresponded to transcripts that could be classified in general categories of low, moderate, or high abundance. These expression profiles were integrated with cDNA clone and sequence clustering and gene mapping information from an upgraded version of the Genexpress Index. For seven gene transcripts found to be transcribed preferentially or specifically in brain, the expression profiles were confirmed by Northern blot analyses of mRNA from eight adult and four fetal tissues, and 15 distinct regions of brain. In four instances, further documentation of the sites of expression was obtained by in situ hybridization of rat-brain tissue sections. A systematic effort was undertaken to further integrate available cytogenetic, genetic, physical, and genic map informations through radiation-hybrid mapping to provide a unique validated map location for each of these genes in relation to the disease map. The resulting Genexpress IMAGE Knowledge Base is illustrated by five examples presented in the printed article with additional data available on a dedicated Web site at the address http://idefix.upr420.vjf.cnrs.fr/EXPR++ +/ welcome.html. (+info)
Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (2/9359)Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space. (+info)
Cloning of a bovine orphan transporter and its short splicing variant. (3/9359)We have isolated a cDNA (bv7-3) encoding a member of the Na+,Cl(-)-dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining. (+info)
The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution. (4/9359)Latrophilin is a brain-specific Ca2+-independent receptor of alpha-latrotoxin, a potent presynaptic neurotoxin. We now report the finding of two novel latrophilin homologues. All three latrophilins are unusual G protein-coupled receptors. They exhibit strong similarities within their lectin, olfactomedin and transmembrane domains but possess variable C-termini. Latrophilins have up to seven sites of alternative splicing; some splice variants contain an altered third cytoplasmic loop or a truncated cytoplasmic tail. Only latrophilin-1 binds alpha-latrotoxin; it is abundant in brain and is present in endocrine cells. Latrophilin-3 is also brain-specific, whereas latrophilin-2 is ubiquitous. Together, latrophilins form a novel family of heterogeneous G protein-coupled receptors with distinct tissue distribution and functions. (+info)
Identification of the human melanoma-associated chondroitin sulfate proteoglycan antigen epitope recognized by the antitumor monoclonal antibody 763.74 from a peptide phage library. (5/9359)To identify the epitope of the melanoma-associated chondroitin sulfate proteoglycan (MCSP) recognized by the monoclonal antibody (mAb) 763.74, we first expressed random DNA fragments obtained from the complete coding sequence of the MCSP core glycoproteins in phages and selected without success for binders to the murine mAb 763.74. We then used a library of random heptapeptides displayed at the surface of the filamentous M13 phage as fusion protein to the NH2-terminal portion of the minor coat protein III. After three rounds of selection on the bound mAb, several phages displaying related binding peptides were identified, yielding the consensus sequence Val-His-Leu-Asn-Tyr-Glu-His. Competitive ELISA experiments showed that this peptide can be specifically prevented from binding to mAb 763.74 by an anti-idiotypic MK2-23 mouse:human chimeric mAb and by A375 melanoma cells expressing the antigen MCSP. We screened the amino acid sequence of the MCSP molecule for a region of homology to the consensus sequence and found that the amino acid sequence Val-His-Ile-Asn-Ala-His spanning positions 289 and 294 has high homology. Synthetic linear peptides corresponding to the consensus sequence as well as to the MCSP-derived epitope inhibit the binding of mAb 763.74 to the phages displaying the consensus amino acid sequence. Finally, the biotinylated consensus peptide absorbed to streptavidin-microtiter plates can be used for the detection of mAb 763.74 in human serum. These results show clearly that the MCSP epitope defined by mAb 763.74 has been identified. (+info)
Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network. (6/9359)SNARE proteins are known to play a role in regulating intracellular protein transport between donor and target membranes. This docking and fusion process involves the interaction of specific vesicle-SNAREs (e.g. VAMP) with specific cognate target-SNAREs (e.g. syntaxin and SNAP-23). Using human SNAP-23 as the bait in a yeast two-hybrid screen of a human B-lymphocyte cDNA library, we have identified the 287-amino-acid SNARE protein syntaxin 11. Like other syntaxin family members, syntaxin 11 binds to the SNARE proteins VAMP and SNAP-23 in vitro and also exists in a complex with SNAP-23 in transfected HeLa cells and in native human B lymphocytes. Unlike other syntaxin family members, no obvious transmembrane domain is present in syntaxin 11. Nevertheless, syntaxin 11 is predominantly membrane-associated and colocalizes with the mannose 6-phosphate receptor on late endosomes and the trans-Golgi network. These data suggest that syntaxin 11 is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network in mammalian cells. (+info)
Caffeine can override the S-M checkpoint in fission yeast. (7/9359)The replication checkpoint (or 'S-M checkpoint') control prevents progression into mitosis when DNA replication is incomplete. Caffeine has been known for some time to have the capacity to override the S-M checkpoint in animal cells. We show here that caffeine also disrupts the S-M checkpoint in the fission yeast Schizosaccharomyces pombe. By contrast, no comparable effects of caffeine on the S. pombe DNA damage checkpoint were seen. S. pombe cells arrested in early S phase and then exposed to caffeine lost viability rapidly as they attempted to enter mitosis, which was accompanied by tyrosine dephosphorylation of Cdc2. Despite this, the caffeine-induced loss of viability was not blocked in a temperature-sensitive cdc2 mutant incubated at the restrictive temperature, although catastrophic mitosis was prevented under these conditions. This suggests that, in addition to S-M checkpoint control, a caffeine-sensitive function may be important for maintenance of cell viability during S phase arrest. The lethality of a combination of caffeine with the DNA replication inhibitor hydroxyurea was suppressed by overexpression of Cds1 or Chk1, protein kinases previously implicated in S-M checkpoint control and recovery from S phase arrest. In addition, the same combination of drugs was specifically tolerated in cells overexpressing either of two novel S. pombe genes isolated in a cDNA library screen. These findings should allow further molecular investigation of the regulation of S phase arrest, and may provide a useful system with which to identify novel drugs that specifically abrogate the checkpoint control. (+info)
Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (8/9359)A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided. (+info)
Subtractive cDNA Library Construction Service - Creative Biogene
Frontiers | Evaluation of Rapid Library Preparation Protocols for Whole Genome Sequencing Based Outbreak Investigation | Public...
Whole genome sequencing (WGS) has become the new gold standard for bacterial outbreak investigation, due to the high resolution available for typing. While sequencing is currently predominantly performed on Illumina devices, the preceding library preparation can be performed using various protocols. Enzymatic fragmentation library preparation protocols are fast, have minimal hands-on time, and work with small quantities of DNA. The aim of our study was to compare three library preparation protocols for molecular typing: Nextera XT (Illumina); Nextera Flex (Illumina); and QIAseq FX (Qiagen). We selected 12 ATCC strains from human Gram-positive and Gram-negative pathogens with %G+C-content ranging from 27% (Fusobacterium nucleatum) to 73% (Micrococcus luteus), each having a high quality complete genome assembly available, to allow in-depth analysis of the resulting Illumina sequence data quality. Additionally, we selected isolates from previously analyzed cases of vancomycin-resistant Enterococcus faecium
low yield in library preparation: post #1
low yield in library preparation - posted in ChIP and Next Generation Sequencing: I got a very successful ChIP (DNA sheared to enrichment around 200 bp, more than 2% input at positive controls). Then I used 10 ng ChIPed DNA to make library for sequencing with the NEB kit. But I got a very poor yield (0.4 ng/ul in 30 ul elutes). I repeated twice with the same DNA and the results didnt turn any better. I used the same kit to make another library two months ago and it worked pretty fine....
cDNA Library Construction Kits | Thermo Fisher Scientific
SeqOnce Biosciences RhinoSeq Rapid DNA Library Preparation Kit | GenomeWeb
SeqOnce Biosciences has launched its RhinoSeq DNA Library Preparation Kit for next-generation sequencing sample preparation. The firm said that the five tube kit contains preformatted master mixes for a simple, fast, and stable workflow. The tool generates a temporary structure that creates sequence-specific single stranded overhangs for sequencing adaptor litigation. When
SPRIselect, Size Selection for Library Preparation - Beckman Coulter
OGT Expands Product Portfolio with Complete Library Preparation Solution
Illumina Introduces Automated Library Preparation - Bio-IT World
By Bio-IT World Staff. February 23, 2015 , This morning, DNA sequencing company Illumina released a new product, NeoPrep, to work alongside its line of next-generation sequencers. NeoPrep is a benchtop device that automatically prepares a DNA or RNA sample for sequencing on Illumina instruments, usually a time-consuming manual process that involves shearing the sample into short fragments, repairing the fragment ends, and linking them to Illumina-specific adapter sequences to create a library of size-selected DNA molecules. Illuminas new instrumentation allows this work to be performed within the NeoPrep device, on a microfluidic cartridge called a NeoPrep library card, in which up to 16 samples in parallel can be processed through each step of the library prep process. While creating libraries will still take between four and eight hours using NeoPrep, lab technicians only have to be present at the very beginning and end of the process, allowing overnight runs and freeing scientists to spend ...
Whole genome sequencing libraries in under 4 hours
RhinoSeq ABRF Poster 2019 - A 12-Min, Single Tube, Nanogram Input, Library Prep - SeqOnce
Current next Generation Sequencing (NGS) library construction is a bottleneck in the sequencing workflow. The processes of library construction for DNA and RNA sequencing are complex, error prone, costly, and time consuming. Current commercial products are multistep, multi-hour sample processing that tend to be cost and labor prohibitive. These issues have created a demand for a simple, rapid, and cost-effective library construction product that offers users application flexibility while minimizing construction complexity for low to ultra-high throughput sample processing. SeqOnce has developed a novel library technology that is rapid and minimizes sample processing complexity. The five tube RhinoSeq kit is comprised of formatted master mixes for a simple and stable user workflow. The 12 minute library construction uses a single master-mix that when combined with fragmentation and PCR steps produces libraries in less than 50 minutes. A size selection step occurs after PCR and the PCR free ...
To the Editor:. Recent studies have demonstrated that the fragment size of circulating tumor DNA (ctDNA) is shorter than normal cell-free DNA (cfDNA)1 (1, 2). As the most common ctDNA fragment lengths were between 134 bp and 144 bp, whereas the modal cfDNA fragment length was 167 bp (2), it was suggested that the enrichment of shorter plasma fragments might improve analysis of ctDNA. Recently, improved recovery of short plasma DNA fragments by single-stranded DNA (ssDNA) library preparation was reported (3). Therefore, we tested whether ssDNA library preparation results in an enrichment of ctDNA compared to the typically employed double-stranded DNA (dsDNA) libraries.. To this end, we applied an adaptor-ligation method as published by Burnham et al. (3) and prepared ssDNA and dsDNA libraries from 6 plasma samples derived from patients with breast (B7_2, B13_1), colon (C177_2, C177_3), and prostate (P226_4, P226_6), all with late-stage metastatic disease.. We first tested the fragment length ...
help - Molecular Cloning
Weve construct the cDNA library by using CloneMiner cDNA Library Construction Kit (Invitrogen). After the construction, were able to obtain high number of titers. Then, we decide to further the analysis by doing express sequence tag (EST). Anyway, were having problem to get the plasmid. We didnt get any plasmid after extraction. The LB broth, LB agar and antibiotic which is kanamycin are in good condition. Theres some situation, that even the bacteria are having problem to grow in the LB broth. Do you have any suggestion? At the moment, I dont have any ideas how to troubleshoot this problem. And my friends also didnt have any clue as this problem is quite strange to us.. ...
Improved genome sequencing using an engineered transposase | BMC Biotechnology | Full Text
Library construction plays an important role for high-throughput next generation sequencing (NGS). Plethora of library construction methods have been developed in the past few years [1-3]. Most common library preparation methods follow a basic procedure with minor variations . This procedure is usually lengthy and includes several steps. First, DNA is fragmented by sonication, nebulization or shearing to desired sizes. The fragmented DNA is then repaired and end-polished including blunt-end and A-tailing. Finally, platform-specific adaptors are ligated to DNA library [2, 4]. Typically, this process results in significant sample loss, and therefore, requires a DNA input amount of over 200 ng, sometimes up to 1 ug. This workflow also limits the throughput. Nextera, developed by Epicentre (an Illumina company), is an alternative approach to streamline the workflow, improve turnaround time and reduce DNA input and increase throughput. Nextera takes advantages of an in vitro transposition ...
Reverse Transcription Technology
There are three choices of RT primer: oligo(dT), random primers, or a gene-specific primer. The priming strategy has a strong impact on the workflow applied, since each priming method has prerequisites and consequences.. Oligo(dT) priming is used to receive full-length copies of the mRNA. For cDNA library construction or cDNA labeling applications, oligo(dT) primers are almost always used to prime cDNA synthesis. Anchored oligo(dT)18 primers are designed to bind at the beginning of the poly(A) tail (rather than randomly within the often long tail) to generate full-length cDNAs. This avoids mispriming and unnecessary reverse transcription of the long poly(A) tail. Since the 5´ ends of long mRNAs are often underrepresented in cDNA mixtures, this primer is preferred for most applications.. However, if the message is long or does not have a poly(A) tail (as with prokaryotic mRNA), then random hexamer primers are used. Random hexamer primers bind throughout the entire length of RNA, ensuring reverse ...
best polyA+ from total RNA method??
In article ,Pine.DYN.3.91.950209140740.15540A-100000 at uxa.cso.uiuc.edu, hodges bradley l ,segdoh at uxa.cso.uiuc.edu, writes: ,From: hodges bradley l ,segdoh at uxa.cso.uiuc.edu, ,Subject: Re: best polyA+ from total RNA method?? ,Date: Thu, 9 Feb 1995 14:09:10 -0600 ,Qiagen sells dT conjugated glass beads (Ithink). , ,On 1 Feb 1995 neale at mbcf.stjude.org wrote: ,, I was wondering if anyone had a strong recommendation of method for ,, purification of poly A+ RNA from total RNA. The samples of total RNA that we ,, have are about 300 ug each. We could pool some if necessary. ,, ,, We could purify poly A+ directly from cells if that is thought superior to ,, purifying from total RNA. Its just that we already have the total RNA, and ,, know that our gene of interest is expressed in these samples. ,, ,, We wish to use the poly A+ RNA for cDNA library construction. Its been a while ,, (5 years) since Ive had to do this and so I thought there may be an improved ,, method for selection of poly A+ ...
SMB DH5α Competent Cells [DH5α] | star-moon
產品說明:The most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. Carries recA1 mutation that eliminates homologous recombination ensuring insert stability. Carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. Useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb). GenotypeF¯ endA1 hsdR17(rk¯, mk¯) supE44 thi-1 λ¯recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 Φ80d lacZ ΔM15 deoR 特色: Suitable for cloning with large plasmid and cDNA library construction Allows blue-white colony screening. 應用: Cloning and subcloning Scale-up application Blue/white screening
Celemics, Inc. | All-in-one Library Preparation Kit
Celemics, Inc. | Library Preparation Kit
A Complete Solution for RNA-Seq Library Construction from Picogram Inputs
Xenbase Library: Summary for NICHD XGC Emb2 [species: Xenopus laevis]
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. NOTE: based on EST sequencing of several thousand clones from this library, there appears to be a low level of mouse background in this library; please use care when evaluating sequences from this library. Note: this is a Xenopus Gene Collection library. ...
Frontier Library: 2010
Today begins National Library Week, an annual celebration that highlights the value of all types of libraries and librarians. This years theme is "Communities Thrive @your library." Libraries have long been integral to communities. They provide access to information for the entire community and also serve as meeting places and community centers. As we strive to build a community of inquiry here at Frontier, the library plays a central role in that process as well. Once you become a part of the Frontier community, you will always be a part of it, no matter where you may reside. But you are also a part of your home community. I thought we could take this opportunity to discuss libraries in your own community. Are you aware of the libraries in your community? If not, you might want to start by checking out www.worldcat.org. On their Find a Library page, you can enter a zip code or city name and see a list of libraries and the distance to them. Note that ALL types of libraries are shown, even ...
Roche KAPA RNA HyperPrep | GenomeWeb
Roche has launched KAPA RNA HyperPrep, a robust RNA library preparation solution for a range of sample types and input amounts. These kits provide a single-tube workflow and decrease processing time by 30 percent while maintaining quality, Roche said. The kits utilize a novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality RNA-seq libraries, Roche added. These strand-specific workflows can be completed in a standard workday while generating high-quality libraries from challenging samples such as formalin-fixed, paraffin-embedded tissue.
Rx Biosciences » Subtracted cDNA Library
Rx Biosciences prepares subtracted cDNA libraries after selectively removing the messages that are present in both, the control as well as the experimental samples. The subtracted library prepared by us is enriched in differentially expressed genes. Using in-house subtraction procedure or other PCR based techniques the libraries are constructed involving rigorous Q.C. at all the stages of the library construction. The libraries prepared by us are highly suitable for high throughput sequencing projects.. ...
FTBO Anti Vibration Plate with Integrated Power Model,ESC,PDB,5V 12V BEC pdb board for APM2.5/2.6/2.8&PIXHAWK Flight...
restraints for crystallographic refinement. Input is in PDB format. Output is Pymol pdb: Using the Python debugger in Django - Minuti by Mike Tigas. 14 Sep 2010 Tutorial on using pdb, the Python debugger, within the Django runserver script.Illumina Sequencing Library Preparation for Highly Multiplexed . doi:10.1101/pdb.prot5448 Cold Spring Harb Protoc 2010. 2010: pdb.prot5448 ...
Fluidigm | Search
Juno™ is preprogrammed to perform multiple methods with push-button ease. Juno methods include targeted DNA sequencing library preparation, IFC control and thermal cycling for SNP genotyping upstream of Biomark™ HD or EP1™ systems, and IFC control for gene expression, copy number analysis or digital PCR upstream of Biomark HD. …. ...
Fluidigm | Search
Juno™ is preprogrammed to perform multiple methods with push-button ease. Juno methods include targeted DNA sequencing library preparation, IFC control and thermal cycling for SNP genotyping upstream of Biomark™ HD or EP1™ systems, and IFC control for gene expression, copy number analysis or digital PCR upstream of Biomark HD. …. ...
Rx Biosciences » Normalized cDNA Library
Rx Biosciences constructs normalized cDNA libraries in which high-copy gene sequences are selectively removed resulting into the enrichment of low copy and rare genes in the library. As per customers request we employ either the technology in which the double stranded cDNA clones of a regular library are converted into single stranded circles, hybridized with cRNA and unhybridized singled stranded circular plasmids are converted into double stranded plasmids or the normalization of high copy genes is performed at the mRNA level before library construction. PCR based technique is also used as per customers request and sample requirement.. ...
Addgene: DECIPHER Mouse shRNA Libraries - Pathway and Disease Targets
PPT - Section I Gene library and screening PowerPoint presentation | free to download - id: 3c2165-YWNjZ
Gene library construction</span>...
Library Prep and NGS Pricing Descriptions - Genomic Sequencing and Analysis Facility User Support Wiki - UT Austin Wikis
Metagenomics assay: This service starts with normalized (i.e. equal concentration and volume) gDNA and includes amplification using one of the GSAF provided primer sets chosen by the customer as shown on this web page. The service is calibrated to deliver at least 10,000 2x250 bp paired-end sequences from the Illumina MiSeq platform for at least 90% of the samples submitted, assuming there is reasonable amount of DNA to amplify. To minimize jackpot effects, PCR is performed in triplicate for each sample and re-pooled. The library preparation is a two-step process, a gene-specific PCR then a second PCR to add the dual indexes. The PCR targets the bacterial 16S V4/V5 region, the 16S V4 region, or fungal ITS region, as specified by the client. Gel-based QC is performed on a sampling of 10% of the samples post amplification. Genomic DNA is expected as input; the GSAF does not provide DNA extraction services at this time. See this web page for sample input guidelines and note that DNA concentrations ...
matplotlib / Mailing Lists
Hi everyone, I am trying to build the latest svn trunk version of MPL on OS X 10.5. I am getting the following error: ld: in /Developer/SDKs/MacOSX10.4u.sdk/usr/local/lib/libPng.dylib, file is not of required architecture for architecture ppc After googling quite a bit, this seems to be quite a common problem. However, I could still not find an unambiguous solution. I have build libpng from source on my machine. The file output is: [email protected]:/usr/local/lib$ file libpng* libpng.3.dylib: Mach-O dynamically linked shared library i386 libpng.a: current ar archive random library libpng.dylib: Mach-O dynamically linked shared library i386 libpng.la: ASCII English text libpng12.0.dylib: Mach-O dynamically linked shared library i386 libpng12.a: current ar archive random library libpng12.dylib: Mach-O dynamically linked shared library i386 libpng12.la: ASCII English text It appears that the compiler expects that libpng will also have libraries for ppc. What do I do? Do I need to rebuild libpng to be ...
Whole Genome and Hybrid Capture - QIAGEN
QIAGENs library prep solutions enable the generation of high-quality, high conversion rate whole genome libraries suitable for direct sequencing or hybrid capture in as little as 2.5 hours, or from as little as 10pg of bacterial or 100pg of eukaryotic DNA. Discover the ways QIAseq library preparation kits maximize per-sample data quality while streamlining your laboratory processes today! ...
Yeast Two-Hybrid cDNA Library - Mate and Plate Libraries
Library construction -- mechanical shearing
Shaun Tyler (styler at HWC.CA) wrote: : I=92m interested in sequencing a 40 kb plasmid (a native one not a clone) : for which I have a limited amount of material and no restriction map.=20 : Since what I have was hard to come by I=92m hesitant to waste it in : establishing a restriction map in order to subclone more manageable : fragments. Our sequencing capabilities are not a limiting factor so I : was thinking of using a shotgun approach with randomly sheared material : for a library and then closing the gaps by PCR and primer walking.=20 : Unfortunately I have been unable to find any protocols for preparing : mechanically sheared DNA. I know people occasionally use this method : for preparing genomic libraries but I=92m not sure how effective this : would be on something relatively small like a 40 kb plasmid. Any input : would be greatly appreciated. Paul Hengen (what would we do without him?) surveyed this in TIBS (1997) 22, 273-274. A nebulizing approach cited therein can be found close to ...
Can I generate a stable cell line with pTK-GLuc Vector? | NEB
Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample! ...
Does the DNA need to be purified after Antarctic Phosphatase treatment? | NEB
Do you need a faster, more reliable solution for DNA fragmentation and library construction? Our new NEBNext® Ultra™ II FS DNA Library Prep Kit with novel fragmentation reagent meets the dual challenge of generating high quality next gen sequencing libraries from ever-decreasing input amounts AND simple scalability. Learn more and request a sample! ...
Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA...
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such as PCR amplification, are known to introduce biases, a systematic evaluation of biases in WGBS strategies is missing. We perform a comparative analysis of several commonly used pre- and post-bisulfite WGBS library preparation protocols for their performance and quality of sequencing outputs. Our results show that bisulfite conversion per se is the main trigger of pronounced sequencing biases, and PCR amplification builds on these underlying artefacts. The majority of standard library preparation methods yield a significantly biased sequence output and overestimate global methylation. Importantly, both absolute and relative methylation levels at specific genomic regions vary substantially
Consider Bias in NGS Library Preparation | Genohub Blog
Library preparation for next-generation sequencing involves a coordinated series of enzymatic reactions to produce a random, representative collection of adapter modified DNA fragments within a specific range of fragment sizes. The success of next-generation sequencing is contingent on this proper processing of DNA or RNA sample. However as you go from isolating nucleic acid to…
Frontiers | Screening of an E. coli O157:H7 Bacterial Artificial Chromosome Library by Comparative Genomic Hybridization to...
Enterohemorrhagic E. coli (EHEC) O157:H7 can cause serious gastrointestinal and systemic disease in humans following direct or indirect exposure to the bacterium excreted in ruminant feces. The main colonization site of EHEC O157:H7 in cattle is the terminal rectum where the bacteria intimately attach to the epithelium and multiply in the intestinal mucus. This study aimed to identify genomic regions of EHEC O157:H7 that contribute to colonization and multiplication at this site. A bacterial artificial chromosome (BAC) library was generated from a derivative of the sequenced E. coli O157:H7 Sakai strain. The library contains 1152 clones averaging 150 kbp. To verify the library, clones containing a complete locus of enterocyte effacement (LEE) were identified by DNA hybridization. In line with a previous report, these did not confer a type III secretion capacity to the K-12 host strain. However, conjugation of one of the BAC clones into a strain containing a partial LEE deletion restored type III
Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
Custom-defined oligonucleotide collections have a broad range of applications in fields of synthetic biology, targeted sequencing, and cytogenetics. Also, they are used to encode information for technologies like RNA interference, protein engineering and DNA-encoded libraries. High-throughput parallel DNA synthesis technologies developed for the manufacture of DNA microarrays can produce libraries of large numbers of different oligonucleotides, but in very limited amounts. Here, we compare three approaches to prepare large quantities of single-stranded oligonucleotide libraries derived from microarray synthesized collections. The first approach, alkaline melting of double-stranded PCR amplified libraries with a biotinylated strand captured on streptavidin coated magnetic beads results in little or no non-biotinylated ssDNA. The second method wherein the phosphorylated strand of PCR amplified libraries is nucleolyticaly hydrolyzed is recommended when small amounts of libraries are needed. The third
HyperCap products provide an integrated next-generation sequencing (NGS) sample preparation solution that combines all reagents and accessories required for library preparation and target enrichment in a single workflow. The HyperCap Workflow v2.0 integrates high-efficiency KAPA Library Preparation Kits and KAPA Dual-Indexed Adapters with performance-optimized SeqCap® EZ probes and Roche Universal Blocking Oligo (UBO) Kit. The HyperCap Workflow v2.0 is an improved and streamlined version of our SeqCap EZ HyperCap Workflow, with the convenience of ordering and support from a single vendor, and is automation friendly.. KAPA HyperPrep and KAPA HyperPlus Library Preparation Kits are novel one-tube chemistry with optimally formulated enzymes selected through our directed evolution technology. They enable higher yields of adapter-ligated library and lower amplification bias resulting in higher library diversity, lower duplication rates and more uniform coverage compared to other products available in ...
Rheonix to Present Data on User-Defined Tests and Next-Generation Sequencing Library Preparation with its Automated Molecular...
Viruses strongly influence microbial population dynamics and ecosystem functions. However, our ability to quantitatively evaluate those viral impacts is limited to the few cultivated viruses and double-stranded DNA (dsDNA) viral genomes captured in quantitative viral metagenomes (viromes). This leaves the ecology of non-dsDNA viruses nearly unknown, including single-stranded DNA (ssDNA) viruses that have been frequently observed in viromes, but not quantified due to amplification biases in sequencing library preparations (Multiple Displacement Amplification, Linker Amplification or Tagmentation).Here we designed mock viral communities including both ssDNA and dsDNA viruses to evaluate the capability of a sequencing library preparation approach including an Adaptase step prior to Linker Amplification for quantitative amplification of both dsDNA and ssDNA templates. We then surveyed aquatic samples to provide first estimates of the abundance of ssDNA viruses.Mock community experiments confirmed ...
A novel process of viral vector barcoding and library preparation enables high-diversity library generation and recombination...
Detailed characterization and mapping of oligonucleotide function in vivo is generally a very time consuming effort that only allows for hypothesis driven subsampling of the full sequence to be analysed. Recent advances in deep sequencing together with highly efficient parallel oligonucleotide synthesis and cloning techniques have, however, opened up for entirely new ways to map genetic function in vivo. Here we present a novel, optimized protocol for the generation of universally applicable, barcode labelled, plasmid libraries. The libraries are designed to enable the production of viral vector preparations assessing coding or non-coding RNA function in vivo. When generating high diversity libraries, it is a challenge to achieve efficient cloning, unambiguous barcoding and detailed characterization using low-cost sequencing technologies. With the presented protocol, diversity of above 3 million uniquely barcoded adeno-associated viral (AAV) plasmids can be achieved in a single reaction through ...
PGN004 PureGenome™ Next Generation Sequencing Library Validator Kit from MilliporeSigma | SelectScience
ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors | Nature Methods
ChIPmentation combines chromatin immunoprecipitation with on-bead tagmentation for rapid and highly robust ChIP-seq library preparation. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase (tagmentation). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.
Fast and Efficient Preparation of Next Generation Sequencing Libraries | SelectScience
Sequencing from a normalized cDNA library of onion revealed 59.9% unique sequences, one of the highest values reported for plant EST collections (Table 1). Large-scale sequencing at TIGR from tissue-specific nonnormalized tomato cDNA libraries revealed ,40% unique ESTs when sequencing ,20,000 random clones. Sampling of EST data sets at TIGR has shown that sequencing fewer clones from different nonnormalized libraries reduced the redundancy to 50% and sequencing from normalized animal cDNA libraries reduced the redundancy to ,30% (Smith et al., 2001). Given continually lower sequencing costs, it may be more cost-effective to sequence fewer random clones from numerous nonnormalized libraries to maximize the return on each sequencing reaction. However, the cost of synthesizing numerous cDNA libraries must be considered, and this approach may not reveal large numbers of relatively low-copy transcripts.. Our onion ESTs represent the first large collection of non-Poales expressed sequences for the ...
Assay Scheme & Configuration of Chromium Single Cell DNA Libraries -Technical Note -Official 10x Genomics Support
CG000162_TechNote_AssayScheme&Configuration_Chromium_scDNA_Libraries_RevA.pdf. This Technical Note presents a detailed description of the assay configuration for Chromium Single Cell DNA libraries. Individual steps during library construction, including primer sequences, adaptor sequences, and the final library construct, are outlined here, and may serve as a reference to customize the library preparation workflow.. FOR USE WITH. ...
An Improved Genotyping by Sequencing (GBS) Approach Offering Increased Versatility and Efficiency of SNP Discovery and...
Highly parallel SNP genotyping platforms have been developed for some important crop species, but these platforms typically carry a high cost per sample for first-time or small-scale users. In contrast, recently developed genotyping by sequencing (GBS) approaches offer a highly cost effective alternative for simultaneous SNP discovery and genotyping. In the present investigation, we have explored the use of GBS in soybean. In addition to developing a novel analysis pipeline to call SNPs and indels from the resulting sequence reads, we have devised a modified library preparation protocol to alter the degree of complexity reduction. We used a set of eight diverse soybean genotypes to conduct a pilot scale test of the protocol and pipeline. Using ApeKI for GBS library preparation and sequencing on an Illumina GAIIx machine, we obtained 5.5 M reads and these were processed using our pipeline. A total of 10,120 high quality SNPs were obtained and the distribution of these SNPs mirrored closely the ...
CATS - a novel library preparation method for ultrasensitive deep sequencing of extracellular circulating RNA and DNA
CDNA library - Wikipedia
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue-specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library. cDNA is created from a mature mRNA from a eukaryotic cell with the use of reverse transcriptase. In eukaryotes, a poly-(A) tail (consisting of a long sequence of adenine nucleotides) distinguishes mRNA from tRNA and rRNA ...
Dr. Matt Rise | Ocean Sciences | Memorial University of Newfoundland
I develop and use genomic resources, including microarrays, in studies related to fish health. DNA microarrays allow a researcher to analyze relative expression levels of thousands of genes simultaneously. In my laboratory, experiments involving genomic techniques are used to identify the key genes involved in biological processes such as reproduction, development, growth, and immune responses to pathogens. We also study the transcriptomic and behavioural responses of fish exposed to environmental stressors including toxicants (e.g. pesticides, heavy metals). Some of the genomic techniques that we utilize include DNA microarray hybridizations, quantitative reverse transcription - polymerase chain reaction (QPCR), and high-complexity cDNA library construction and characterization. ...
"Quantitative comparison of EST libraries requires compensation for sys" by D Liu and J H. Graber
BACKGROUND: Publicly accessible EST libraries contain valuable information that can be utilized for studies of tissue-specific gene expression and processing of individual genes. This information is, however, confounded by multiple systematic effects arising from the procedures used to generate these libraries. RESULTS: We used alignment of ESTs against a reference set of transcripts to estimate the size distributions of the cDNA inserts and sampled mRNA transcripts in individual EST libraries and show how these measurements can be used to inform quantitative comparisons of libraries. While significant attention has been paid to the effects of normalization and substraction, we also find significant biases in transcript sampling introduced by the combined procedures of reverse transcription and selection of cDNA clones for sequencing. Using examples drawn from studies of mRNA 3-processing (cleavage and polyadenylation), we demonstrate effects of the transcript sampling bias, and provide a
Training at your library: we asked, you answered | NoveList Blog | EBSCOhost
Most of the respondents said their library did not have a dedicated trainer or staff development coordinator at their library. The 18% of libraries with one or more full-time trainers were from libraries that serve larger population. For example, 38% of libraries serving 100 thousand to 500 thousand people and 32% of libraries serving over 500 thousand people have full-time trainers.. A couple interesting notes to come out of the question about in-house training: at least one library focuses training on new staff with current staff only receiving training when policies and procedures change. Another library pointed out that they have staff members train on their expertise, from example youth services coordinators train on youth topics and human resources coordinates safety training, onboarding, and other system-wide concerns.. Libraries do bring in outside trainers, though again this varies by library size. Larger libraries are more likely to already bring in outside trainers or to plan to in ...
Re)Claiming Space: Two Subject Libraries at the Heart of the Academic Enterprise - Association of Collegiate Schools of...
Lucy Campbell and Barbara Opar, column editors. Column by David Eifler, Librarian, Environmental Design Library, UC Berkeley and Maya Gervits, Director, Littman Architecture Library, NJIT. (Re)Claiming Space: Two Subject Libraries at the Heart of the Academic Enterprise. The development of digital technology has led to predictions that the library as a physical space will cease to exist. However, the physical world of libraries has not been replaced by a digital one, rather they are developing in parallel. Architect Geoffrey Freeman acknowledges that "Whereas the Internet has tended to isolate people, the library, as a physical place, has done just the opposite." Library scholar Michael Gorman notes that among other things, libraries are valued for being a focal point of a community, the heart of the university, the collective memory of a research institution, a place in which all are welcome and a source of power through knowledge.. University art and architecture libraries are no different and ...
Xenbase Library: Summary for NIH XGC tropOvi1 [species: Xenopus tropicalis]
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3) and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5-AATTCGGCACGAGG-3) followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). ...
Difference between revisions of "Library Generation" - OpenWetWare
Genetic libraries are collections of genes present in some recombinant DNA form so they can be propagated. When people refer to "screening a library" they usually have some phenotype that they are able to select or screen for and evaluate a large number of library clones to look for a gene that alters the phenotype. People interested in eukaryotic biology usually make cDNA libraries that are derived from pools of mRNA isolated from an organism of interest. This allows them to isolate DNA fragments that encode proteins or RNA that are produced form the spliced form of the RNAs found in the cells. It has been a long time since I have worked with cDNA libraries, so I wont go into that here (perhaps someone in another group can add a section?). For example, suppose we have a strain of bacteria that cant grow on lactose (like Salmonella) and we are interested in finding genes that are needed for lactose metabolism. First, we prepare a plasmid that has been digested with two different restriction ...
Stranded RNA-seq - QIAGEN
For applications such as gene expression, fusion gene or mutation detection, QIAseq Stranded mRNA Select Kits include an optimized mRNA enrichment protocol with all the reagents and components required to build high-quality RNA-seq libraries. The QIAGEN proprietary CleanStart PCR Mix included with kits efficiently and uniformly amplifies the RNA-seq library regardless of the GC content of the template, while also protecting against PCR contamination. Kits are compatible with fresh, as well as FFPE samples. The streamlined, 4-5 hour protocol allows the generation of NGS libraries, library QC measurements and the start of an NGS run in just one working day ...
Comparison of RNA Expression Profiles Based on Maize Expressed Sequence Tag Frequency Analysis and Micro-Array Hybridization |...
Fig. 1. Comparison of TUG overlap between three pairs of cDNA libraries. The first section (starting at the top and moving counterclockwise) of the larger pie chart in each panel represents the singlets unique to the second library. The second section represents the singlets unique to the first library. The third and fourth sections represent the contigs unique to the second and first libraries, respectively. The smaller pie charts in each panel represent the TUGs containing ESTs from both libraries. The three sections of each smaller pie chart represent TUGs with one EST from both libraries ("SS → C"), one EST from one library, and multiple ESTs from the second library ("SC → C"), and multiple ESTs from both libraries ("CC → C"). In A, 1-mm tassel primordia (library 946) is compared with 0.5- to 2.0-cm tassels encompassing stages of organ specification and early differentiation (library 618). In B, library 618 is compared with 1- to 2-cm immature ear (library 606) containing similar ...
Services Overview | GEGF
Genotyping & Genetic Variation. ABI TaqMan Chemistry. Affymetrix 6.0 Genotyping Arrays. Affymetrix Axiom Chemistry. Affymetrix Oncoscan Arrays. . Library Preparation & NGS Support. Agilent SureSelect Library Prep & Capture. Illumina TruSeq Library Prep & Capture. Validation of NGS Identified Variants. . DNA Fragment Analysis, Quantitation & Isolation. Agilent Tape Station 2200. Nanodrop/Qubit. . FFPE Sample Support. FFPE DNA Extraction. FFPE DNA Quantitation & Quality Assessment. Affymetrix Microarray Analysis. Library Prep & Capture for NGS ...
2157 (Purpose) We previously reported that heat shock protein 105 (HSP105) was identified by serological analysis of a recombinant cDNA expression library (SEREX) using serum from a pancreatic cancer patient and HSP 105 was overexpressed in various human tumors and in the testis of adult men by immunohistochemical analysis. The aim of this study is to examine the expression HSP 105 in relation to breast cancer.. (Experimental Design) We examined, by immunohistochemical analysis, the expression of HSP 105 in normal breast tissue, benign disorders and breast cancer. In breast cancer, we investigated the correlation between expression of HSP 105 and clinicopathological and biological factors. We also studied the prognostic value of HSP 105 in breast cancer.. (Results) HSP 105 was mainly detected in cytoplasm of epithelium. In normal breast tissue, no detectable immunoreactive HSP 105 was found in ductal epithelium, but HSP 105 was detected weakly in some lobule. In benign disorder such as ...
Membrane digestion : new facts and concepts (Work) - University of Missouri Libraries
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Fungi (Topic) - University of Oklahoma Libraries
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Eye -- Wounds and injuries | Surgery (Concept) - University of Missouri Libraries
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Health Surveys (Concept) - University of Oklahoma Libraries
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Detroit: Detroit Library Commission. Scott, Gene (2001). Detroit Beginnings: Early Villages and Old Neighborhoods. Detroit: ... Chicago: American Library Association. ISBN 0838906435. Ewen, Lynda Ann (1978). Corporate Power and Urban Crisis in Detroit. ... Bentley Historical Library. Retrieved on January 8, 2014. McGrail, Lucas. The Carlton. Historic Detroit. Retrieved April 1, ...
Transmembrane protein 251
access-date= requires ,url= (help) "TMEM251 transmembrane protein 251 [Homo sapiens (human)]". NCBI Gene. U.S. National Library ... which is a set of similar genes that are formed by duplication of an original gene, is around 120 genes. Gene duplication, ... The TMEM251 gene is located on human chromosome 14, at 14q32.12, on the plus strand. The gene size is 1,277 base pairs. It ... The TMEM251 gene first appeared on the planet around 400 million years ago (MYA), since the most distant orthologs are found in ...
Rap GTP-binding protein
Entrez Gene. United States National Library of Medicine. Rousseau-Merck MF, Pizon V, Tavitian A, Berger R (1990). "Chromosome ... Human genes that encode Ras-related proteins include: RAP1A, RAP1B RAP2A, RAP2B, RAP1C RAB5C "RAP1A RAP1A, member of RAS ... rap GTP-Binding Proteins at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ... from the United States National Library of Medicine, which is in the public domain. Molecular and Cellular Biology portal. ...
"Entrez Gene: IYD Iodotyrosine deiodinase". Entrez Gene. United States National Library of Medicine. Rosenberg IN, Goswami A ( ... Moreno JC (2003). "Identification of novel genes involved in congenital hypothyroidism using serial analysis of gene expression ... The gene encoding this enzyme has been recently identified. The sequence of amino acids of iodotyrosine deiodinase is highly ... Mutations in the gene encoding iodotyrosine deiodinase can affect enzyme function and be detrimental to human health. Iodide is ...
"OPN1LW gene". U.S National Library of Medicine. Genetics Home Reference. Retrieved November 29, 2017. Imamoto Y, Shichida Y ( ... Protanomaly occurs when a partially functional hybrid OPN1LW gene replaces the normal gene. Opsins made from these hybrid genes ... regulates expression of both genes, with only the OPN1LW gene and nearby adjacent OPN1MW genes being expressed and contributing ... They are in a tandem array, composed of a single OPN1LW gene which is followed by one or more OPN1MW genes.The locus control ...
Eastern New Mexico University
One Thousand Ways to Make $1000
Jehovah's Witnesses publications
Libraries Unlimited. Eugene V. Gallagher; W. Michael Ashcraft (2006). Introduction to New and Alternative Religions in America ... Others, such as the organizational manual Organized to Do Jehovah's Will and Watchtower Library CD-ROM (containing the Watch ... Many of these publications are also available from their website in the Watchtower Online Library. In addition to Jehovah's ... but may be supplied to other interested individuals on request or made available in public libraries. These include the ...
Arthur W. Nienhuis
Daniel Sheldon Norton
Ko Ko Mo (I Love You So)
Galen Gart, First Pressings: The History of Rhythm & Blues: Volume 4: 1954 (1954). "Gene & Eunice Record Label Shots", Library ... Gene and Eunice (with Jonesy's Combo) (November, 1954; Combo 64). Gene and Eunice (with Johnny's Combo) (January 17, 1955; ... "Gene & Eunice: This is My Story" "Gene & Eunice Record Label Shots". The Billboard (January 15, 1955):33. ... The Valtairs (1965: Selsom Records s 1061). Gene and Eunice (1973; United Artists Silver Spotlight Series XW151). Gene and ...
Schimmel, Caroline F. (November 1978). "The Man who Printed Books at the YMCA." Columbia Library Columns. Ettenberg, Eugene M ... PA Photography by John Fass at New York Public Library John Fass' Hammer Creek Press Books at New York Public Library John Fass ... John Archer organized an exhibition of Hell-Box Press and Hammer Creek Press imprints in 1953 for the New York Public Library. ... p. 134 Burke, Jackson; Ettenberg, Eugene M. (1998). John S. Fass and the Hammer Creek Press. Melbert B. Cary, Jr. Graphic Arts ...
Peter II, Count of Savoy
London: British Library. 1916. Cox, Eugene L. (1974). The Eagles of Savoy. Princeton: Princeton University Press. ISBN ... Cox, Eugene L. (1967). The Green Count of Savoy. Princeton, New Jersey: Princeton University Press. LCCN 67-11030. Pollock, M. ... British Library 1916, p. 78. Chisholm, Hugh, ed. (1911). "Richmond, Earls and Dukes of". Encyclopædia Britannica. 23 (11th ed ...
Arthur C. Watson
After his death, the NSU library was named in his honor. A national library science scholarship was also founded in Eugene ... "Eugene P. Watson Memorial Library". louisiana.hometownlocator.com. Retrieved January 19, 2012. Arthur C. Watson at Find a Grave ... He married the former Marion Eugenia "Gene" Hickman, and they had three daughters. Watson's brother, Eugene Payne Watson, was ... Watson funded scholarships and other awards for outstanding students at Northwestern in music and library science and was a ...
... genes from genomic libraries". Gene. 237 (1): 61-70. doi:10.1016/S0378-1119(99)00302-9. PMID 10524237. Cal S, Freije JM, López ... Gene. 325: 171-8. doi:10.1016/j.gene.2003.10.012. PMID 14697522. Schmitt-Ulms G, Hansen K, Liu J, Cowdrey C, Yang J, DeArmond ... This gene is highly expressed in the brain and may function as an integrin ligand in the brain. GRCh38: Ensembl release 89: ... This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane- ...
... genes from genomic libraries". Gene. 237 (1): 61-70. doi:10.1016/S0378-1119(99)00302-9. PMID 10524237. "Entrez Gene: ADAM22 ... This gene is highly expressed in the brain and may function as an integrin ligand in the brain. Alternative splicing results in ... Gödde NJ, D'Abaco GM, Paradiso L, Novak U (November 2007). "Differential coding potential of ADAM22 mRNAs". Gene. 403 (1-2): 80 ... The MEROPS online database for peptidases and their inhibitors: M12.978 Human ADAM22 genome location and ADAM22 gene details ...
Romania in World War II
Bachman, Ronald D.; Keefe, Eugene K. Area handbook for Romania; Library of Congress. Federal Research Division (1991). Romania ... The Library of Congress. Washington, D.C. : The Division : For sale by the Supt. of Docs., U.S. G.P.O. pp. 41.. This article ... 23, Library of Congress *^ (in Romanian) Delia Radu, "Serialul 'Ion Antonescu și asumarea istoriei' (3)", BBC Romanian edition ... A Country Study by the U.S. Federal Research Division of the Library of Congress attributes this to a "morbid competition with ...
Image provided by University of Oregon Libraries; Eugene, OR. Nelson, Mrs. Norman, Mrs. Omar Rietmann, Mrs. Anne Smouse, Mrs. ... Heppner gazette-times., March 07, 1963; University of Oregon Libraries 1501 Kincaid Street Eugene, OR 97403-1299; Image ... University of Oregon Libraries 1501 Kincaid Street Eugene, OR 97403-1299; Image provided by: Morrow County Museum; Heppner, OR ... University of Oregon Libraries 1501 Kincaid Street Eugene, OR 97403-1299; Image provided by: Morrow County Museum; Heppner, OR ...
University of Oregon Libraries; Image provided by: University of Oregon Libraries; Eugene, OR. 9 Oct 1910. Retrieved 11 ... University of Oregon Libraries, Image provided by: University of Oregon Libraries; Eugene, OR (March 23, 1911). "Heppner ... University of Oregon Libraries; Image provided by: Morrow County Museum; Heppner, OR. 3 January 1952. University of Oregon ... University of Oregon Libraries, Image provided by: Morrow County Museum; Heppner, OR (October 4, 1973). "Heppner Gazette-Times ...
D. B. Newton
University of Oregon Libraries. Eugene, Oregon. 2007. Retrieved September 2, 2017 - via Archives West. Dwight Newton on IMDb ... Popular Library (1975) Trail of the Bear, Popular Library (1975) Broken Spur, Berkley (1977) As "Dwight Bennett" Stormy Range, ... 1981) As "Ford Logan" Fire in the Desert, Ballantine Books (1954) As "Dan Temple" Bullet Lease, Popular Library (1957) Gun and ... Nineteen linear feet of the author's papers are held at the University of Oregon Libraries, Special Collections and University ...
Bibliography of encyclopedias: biology
Sheehy, Eugene P., ed. (1986). Guide to Reference Books (Tenth ed.). Chicago and London: American Library Association. ISBN 978 ... Talbot, C. H., Eugene Ashby Hammond. The medical practitioners in medieval England: A biographical register. Wellcome ... Page, Lawrence M., Brooks M. Burr, Eugene C. Beckham III, National Audubon Society. A field guide to freshwater fishes: North ... Distributed in the U.S. to the Bookstore and library trade by National Book Network. p. 960. ISBN 0793806569. ISBN 978- ...
Bibliography of encyclopedias: religion
Sheehy, Eugene P., ed. (1986). Guide to Reference Books (Tenth ed.). Chicago and London: American Library Association. ISBN 978 ... Neusner, Jacob, William Scott Green.Dictionary of Judaism in the biblical period: 450 B.C.E. to 600 C.E. Macmillan Library ... Neusner, Jacob, William Scott Green.Dictionary of Judaism in the biblical period: 450 B.C.E. to 600 C.E. Macmillan Library ... ISBN 0-415-92472-3. A Latter-Day Saint library: Including the encyclopedia of Mormonism/Church of Jesus Christ of Latter-Day ...
This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... This article on a gene on human chromosome 19 is a stub. You can help Wikipedia by expanding it.. *v ... Gene ontology. Molecular function. • transcription factor activity, sequence-specific DNA binding. • DNA binding. • ... Stubbs L, Carver E, Ashworth L, Lopez-Molina L (Jan 1996). "Location of the DBP transcription factor gene in human and mouse". ...
Structure-based combinatorial protein engineering
To create the corresponding library of genes, the breeding scheme of Gregor Mendel was adapted into a PCR strategy to ... These examples establish SCOPE as a standardized method for the construction of synthetic gene libraries from close or ... O'Maille PE, Tsai MD, Greenhagen BT, Chappell J, Noel JP (2004). "Gene library synthesis by structure-based combinatorial ... Structure-based combinatorial protein engineering (SCOPE) is a synthetic biology technique for creating gene libraries ( ...
Cambridge Antibody Technology
Human antibodies from V-gene libraries displayed on phage". Journal of Molecular Biology. 222 (3): 581-97. doi:10.1016/0022- ... Free Online Library". Thefreelibrary.com. Retrieved 2010-05-12. Foley, Stephen (27 March 2003). "CAT's bid hopes floored by ... Free Online Library". Thefreelibrary.com. 2004-02-01. Retrieved 2010-05-12. "Amgen - Media - Press Release". Xt.amgen.com. ... This should make it possible to construct libraries that are orders of magnitude larger than those created using phage display ...
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Death-associated protein 6
... end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Ishov AM, Sotnikov AG, ... This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. Daxx serves ... Death-associated protein 6 also known as Daxx is a protein that in humans is encoded by the DAXX gene. Daxx, a Death domain- ... "Entrez Gene: DAXX". Salomoni P, Khelifi AF (2006). "Daxx: death or survival protein?". Trends Cell Biol. 16 (2): 97-104. doi: ...
Cordon-bleu protein-like 1
... end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Nagase T, Ishikawa K, ... "Entrez Gene: COBLL1 COBL-like 1". Human COBLL1 genome location and COBLL1 gene details page in the UCSC Genome Browser. ... Carroll EA, Gerrelli D, Gasca S, Berg E, Beier DR, Copp AJ, Klingensmith J (October 2003). "Cordon-bleu is a conserved gene ... Cordon-bleu protein-like 1 is a protein that in humans is encoded by the COBLL1 gene. GRCh38: Ensembl release 89: ...
... end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Chang BH, Mukherji S, ... Calcium/calmodulin-dependent protein kinase type II beta chain is an enzyme that in humans is encoded by the CAMK2B gene. The ... The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular ... Human CAMK2B genome location and CAMK2B gene details page in the UCSC Genome Browser. Molecular and Cellular Biology portal. ...
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人類基因組 - 维基百科，自由的百科全
Find this book in the Eugene, Oregon Public Library catalog. * Find this book in the Multnomah County, Oregon Library catalog ... Public libraries. * Find this book (and library resources in other formats) at your local library through the National Library ... Find this book at the Gothenburg Public Library library catalogue. * Find this book at the Malmö City Library library catalogue ... Public libraries. * Find this book in the Barrie Public Library catalogue. * Find this book in the Burnaby Public Library ...
"Mean" Gene Kelton, singer-songwriter, blues musician, and band leader of Mean Gene Kelton & The Die Hards ... The current Sterling Library was dedicated in 1963. The library's space increased to 50,500 square feet (4,690 m2) after bond ... The original Goose Creek Library opened in 1925; the first county library in Texas, it was funded by the private donations of ... Located on the back corner of the library on W. Texas Avenue, this is a life-sized book shelf. The second ...
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on ... This article on a gene on human chromosome 2 is a stub. You can help Wikipedia by expanding it.. *v ...
"Entrez Gene: NMT1 N-myristoyltransferase 1". Rajala RV, Datla RS, Moyana TN, et al. (2000). "N-myristoyltransferase". Mol. Cell ... 1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107-13. doi:10.1006/abio. ... is an enzyme that in humans is encoded by the NMT1 gene. GRCh38: Ensembl release 89: ENSG00000136448 - Ensembl, May 2017 GRCm38 ...
These gene products are important in determining cell fates during normal development and in maintaining homeostasis, or they ... Catenins at the US National Library of Medicine Medical Subject Headings (MeSH). ... Mutations in genes encoding these proteins can lead to inactivation of cadherin cell adhesions and elimination of contact ... Mutations in catenin genes can cause loss of contact inhibition that can promote cancer development and tumor formation. ...
It has a core set of 1553 genes, plus 154 genes in its virulome, which contribute to virulence and 176 genes that maintain a ... PATH's Vaccine Resource Library pneumococcal resources. *. Centers for Disease Control and Prevention (2012). "Ch. 16: ... For instance, the Xisco gene was recently described as a biomarker for PCR-based detection of S. pneumoniae and differentiation ... In S. pneumoniae, at least 23 genes are required for transformation. For a bacterium to bind, take up, and recombine exogenous ...
... Research Archive Collection Guide - University of Iowa Libraries' Rita Benton Music Library. ... Eugene Rousseau, the classical saxophonist, is one of his former students. Much of his music was published by Rubank, Inc. ... University of Iowa Libraries. Spring 2004. Archived from the original (PDF) on 2008-06-25. Retrieved 2008-02-26.. ...
"Identification of significant association and gene-gene interaction of GABA receptor subunit genes in autism". Am. J. Hum. ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ McLean PJ, Farb DH, Russek SJ (Aug 1995 ... Gamma-aminobutyric acid receptor subunit alpha-4 is a protein that in humans is encoded by the GABRA4 gene. ...
America in the King Years
These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ... U.S. National Library of Medicine. Retrieved 7 June 2020.. *^ Zhanel G, Critchley I, Lin LY, Alvandi N (January 2019). " ... Genes. Acne appears to be strongly inherited; genetics explain 81% of the variation in the population. Studies ... among others. The 308 G/A single nucleotide polymorphism variation in the gene for TNF is associated with an increased risk ...
"Fibromyalgia Library. ProHealth.com. Retrieved 2008-11-01.. *Fight Club for Flies video, Science Take, New York Times, February ... organization of the gene, chromosome localization, and functional expression of cDNA clones". Biochemistry. 30 (44): 10640-6. ... are produced from a polyprotein precursor after differential splicing of the preprotachykinin A gene. The deduced amino acid ... "C/EBPbeta couples dopamine signalling to substance P precursor gene expression in striatal neurones". Journal of Neurochemistry ...
Apolipoprotein (apo)C4 gene is a member of the apolipoprotein C gene family. It is expressed in the liver and has a predicted ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... Apo C4 is a 3.3-kb gene consisting of 3 exons and 2 introns; it is located 0.5 kb 5' to the APOC2 gene. ... This article on a gene on human chromosome 19 is a stub. You can help Wikipedia by expanding it. *v ...
Library of Congress ID: 61-11617.. *^ Vaisrub, Samuel; A. Denman, Michael; Naparstek, Yaakov; Gilon, Dan (2008). "Medicine". ... Many modern molecular tests such as flow cytometry, polymerase chain reaction (PCR), immunohistochemistry, cytogenetics, gene ... Genetics is the study of genes, and their role in biological inheritance. ... as the causative genes of most monogenic genetic disorders have now been identified, and the development of techniques in ...
... end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.. ... Paired box gene 8, also known as PAX8, is a protein which in humans is encoded by the PAX8 gene. ... This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ...
"Modern Library. 1998. Retrieved March 4, 2015.. *^ a b Brian Urquhart (March 26, 2009). "What You Can Learn from Reinhold ... "Reinhold Niebuhr, Washington's Favorite Theologian" by Gene Zubovich, "Religion & Politics", April 25, 2017 ... The latter is ranked #18 of the Top 100 non-fiction books of the twentieth century by Modern Library. Andrew Bacevich ... Works by or about Reinhold Niebuhr in libraries (WorldCat catalog). *"The Ironic Element in the American Situation", an excerpt ...
List of tuberculosis cases
Človek neandertálsky - Wikipédia
"National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ Misrahi M, Atger M, d'Auriol L, Loosfelt ... In humans, PR is encoded by a single PGR gene residing on chromosome 11q22, it has two isoforms, PR-A and PR-B, that ... ensembl.org, Gene: ESR1 (ENSG00000091831) *^ Gadkar-Sable S, Shah C, Rosario G, Sachdeva G, Puri C (2005). "Progesterone ... Gene ontology. Molecular function. • DNA binding. • sequence-specific DNA binding. • transcription factor activity, sequence- ...
1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1-2): 149- ... Uridine-cytidine kinase-like 1 is an enzyme that in humans is encoded by the UCKL1 gene. GRCh38: Ensembl release 89: ... 2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome ... "Entrez Gene: UCKL1 uridine-cytidine kinase 1-like 1". Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based ...
PPT - Section I Gene library and screening PowerPoint presentation | free to download - id: 3c2165-YWNjZ
... library and screening I1 Genomic libraries I2 cDNA libraries I3 Screening procedures I1 Genomic libraries Representative gene ... Gene libraries and screening*Representative gene libraries Gene libraries made from genomic DNA are called genomic libraries ... Section I Gene library and screening. Description:. Molecular Biology Section I Gene library and screening I1 Genomic libraries ... Section I Gene library and screening. 1. Section I Gene library and screening Molecular Biology 2. Molecular Biology. Gene ...
Gene library construction</span>...
SMB DH5α Competent Cells [DH5α] | star-moon
Gene Wilburn | Open Library
Gene Townsend | Open Library
Books by Gene Townsend, Whaddayah mean leave home and travel for the rest of my life!, The whaddayah-mean leave-home-and-travel ... Open Library is an initiative of the Internet Archive, a 501(c)(3) non-profit, building a digital library of Internet sites and ... New Feature: You can now embed Open Library books on your website! Learn More ... Are you sure you want to remove Gene Townsend from your list? ... by Gene Townsend 1 edition - first published in 1995 Download ...
Gene Library - MeSH - NCBI
All MeSH CategoriesPhenomena and Processes CategoryGenetic PhenomenaGenetic StructuresGene LibraryGenomic LibraryPeptide ... Gene Library. A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell ... National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA ... It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from ...
Gene Sharp - Wikisource, the free online library
Top Picks: Gene Hackman | Chicago Public Library
Gene Hackman knew from childhood that he wanted to be an actor, but his path was not an easy one. Hackman was born 90 years ago ... Gene Hackman knew from childhood that he wanted to be an actor, but his path was not an easy one. Hackman was born 90 years ago ... Gene Hackman has a rich and lasting legacy, and despite his fame, always saw himself as a "regular guy" who maintained his ... Community-contributed content represents the views of the user, not those of Chicago Public Library ...
Awesome Library - Arts - Music - Jazz - Krupa, Gene
Gene Expression: Safari Library
Narrow Roads of Gene Land 1. Narrow Roads of Gene Land 2. Narrow Roads of Gene Land 3. Statistical Methods in Molecular ... Narrow Roads of Gene Land 1. Narrow Roads of Gene Land 2. Narrow Roads of Gene Land 3. Statistical Methods in Molecular ... Safari Library posted by Razib @ 6/09/2007 10:27:00 PM Safari Library ... The Math Gene. Explaining Culture. Origin and Evolution of Cultures. Dawn of Human Culture. The Origins of Virtue Prehistory of ...
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Novel gene complex structure determination - Digital Library
University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents ... UNT Libraries Government Documents Department Serving as both a federal and a state depository library, the UNT Libraries ... Gatewood, J.M. Novel gene complex structure determination, report, August 1, 1997; New Mexico. (digital.library.unt.edu/ark:/ ... Digital Library, a digital repository hosted by the UNT Libraries. More information about this report can be viewed below. ...
Gene Localization by in situ Hybridization: FISH - eLS - Ekong - Wiley Online Library
Gene Expression Programming: Library & Information Science Book Chapter | IGI Global
Gene expression programming (GEP) introduced by Candida Ferreira is a descendant of genetic algorithm (GA) and genetic ... "Gene Expression Programming." In Bio-Inspired Computing for Information Retrieval Applications. edited by D.P. Acharjya , and ... "Gene Expression Programming." Bio-Inspired Computing for Information Retrieval Applications, edited by D.P. Acharjya and ... Gene Expression Programming. Baddrud Zaman Laskar (NIT Silchar, India) and Swanirbhar Majumder (NERIST, India). ...
Search Results - 'Roland, Gene' - 5 Results - Digital Library
Group Photograph of Gene Roland and Others] Description: The photograph is of Gene Roland, Keith La Motte, Dwight Carver, Carl ... Photograph of Stan Kenton and Gene Roland] Description: The photograph is of Stan Kenton and Gene Roland (arranger) at a ... Photograph of Stan Kenton and Gene Roland] Description: The photograph is of Stan Kenton and Gene Roland (arranger) at a ... Research @ The UNT Libraries Links and search tools for all of the collections and resources available from UNT. ...
Human gene-splice test considered. - Free Online Library
Human gene-splice test considered. by Science News; Science and technology, general Cancer Research Cancer research Genetic ... The Free Library , Science and Technology , Science and technology, general , Science News , June 25, 1988 ... 2020 https://www.thefreelibrary.com/Human+gene-splice+test+considered.-a06831893. *Chicago style: The Free Library. S.v. Human ... MLA style: "Human gene-splice test considered.." The Free Library. 1988 Science Service, Inc. 04 Dec. ...
Geneticists uncover heart disease gene. (atherosclerosis susceptibility, ATHS) (Brief Article) by Science News; Science and ... The Free Library , Science and Technology , Science and technology, general , Science News , August 8, 1992 ... 2017 https://www.thefreelibrary.com/Geneticists+uncover+heart+disease+gene.-a012535765. *Chicago style: The Free Library. S.v. ... MLA style: "Geneticists uncover heart disease gene.." The Free Library. 1992 Science Service, Inc. 16 Oct. ...
Eugene Field Exhibition - The University of Chicago Library
Eugene Field (1850 - 1895) was a major figure in Chicagos literary and book collecting circles at the time of his death 100 ... "Eugene Field and his Books" marks the centenary of Fields death and the founding of Chicagos Caxton Club. The exhibition, ... and the holdings of the University of Chicago Library, focuses on Field as author, collector and promoter of books and the book ... Library Hours Ask a Librarian Support the Library My Library Account Toggle navigation The University of Chicago Library ...
Eugene Judson Hall - Wikisource, the free online library
David Eugene Price - Wikisource, the free online library
Gene Epstein Archives | EconTalk | Library of Economics and Liberty
Gene Epstein on Gold, the Fed, and Money. EconTalk Episode with Gene Epstein Hosted by Russ Roberts ... Gene Epstein, Barrons economics editor, talks to EconTalk host Russ Roberts about the virtues of the gold standard relative to ... CATEGORIES: Gene Epstein , Government Budgets and Taxation , Law and Institutions , Money, Banking, Monetary Policy ... Gene Epstein Podcast Episodes and Extras. Category Archive with 1 podcast episodes and extras ...
Duplicated Genes Database API Libraries | ProgrammableWeb
The Duplicated Genes Database (DGD) provides a list of co-located and duplicated genes. These genes may be either tandem ... Libraries. The following is a list of libraries from ProgrammableWebs Library Directory that matched your search term. ... or to study the duplication status of a specific gene or group of genes. The SOAP-based DGD API can only use Ensembl gene IDs ... Lists of duplicated genes can be used to analyze the expression of genes from microarray profiling experiments, to characterize ...
Gene Spencer • Library and Information Technology • Ursinus
Gene provides leadership for all areas of Library and Information Technology support on campus, including circulation, ... Gene has also served in a variety of IT and joint Library/IT positions at his alma mater, Bucknell University. As a consultant ... Gene provides leadership for all areas of Library and Information Technology support on campus, including circulation, ... Please feel free to contact Gene with any questions or concerns you might have with information technology or library services ...
UK approves gene editing | Bioethics Research Library
UK approves gene editing. This week, UK regulators gave approval to a group of scientists in London to genetically modify human ... Kathy Niakan, the researcher who will be performing the experiments, said, "We would really like to understand the genes needed ... Researchers will alter the genes in donated embryos, then destroy them at age seven days. ... expressed by these authors and blogs are theirs and do not necessarily represent that of the Bioethics Research Library and ...
Eugene Public Library - Wikipedia
Eugene Public Library. Archived from the original (PDF) on April 1, 2010. "About the library." Eugene Public Library. Retrieved ... The Eugene Public Library is a municipal public library in Eugene, Oregon, United States. It has been in four different ... "A Rich History". Eugene Library Foundation. Retrieved April 20, 2014. Lowry, Marian (August 20, 1931). "Eugene Public Library ... Eugene Public Library was established as a tax-supported entity in 1904. In 1906, Oregons first Carnegie Library was ...
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. - PubMed - NCBI
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.. Dietzl G1, Chen D, Schnorrer F, Su KC, ... Our transgenic RNAi library thus opens up the prospect of systematically analysing gene functions in any tissue and at any ... National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA ... Our RNAi transgenes consist of short gene fragments cloned as inverted repeats and expressed using the binary GAL4/UAS system. ...
Cloning thousands of genes for massive protein libraries | EurekAlert! Science News
Until now, this was performed on a one-gene-at-a-time basis, causing a bottleneck. Scientists at Rutgers University-New ... and Harvard Medical School have invented a technology to clone thousands of genes simultaneously and create massive libraries ... Discovering the function of a gene requires cloning a DNA sequence and expressing it. ... Cloning thousands of genes for massive protein libraries. Rutgers University. Journal. Nature Biomedical Engineering. Funder. ...
WikiGenes - Gene Library
Gene context of Gene Library. *Three members of the Jun/AP-1 family have been identified in mouse cDNA libraries: c-Jun, Jun-B ... Anatomical context of Gene Library. *We have screened a cDNA library for gene sequences that are regulated by platelet-derived ... Biological context of Gene Library. *DNA sequence analysis of a class I gene (Q10), which maps to the Qa2,3 locus in the C57BL/ ... Disease relevance of Gene Library. *Phage subclones from the breakpoint region were isolated and used to screen cDNA libraries ...
Pollen and gene flow in fragmented habitats - Kwak - 1998 - Applied Vegetation Science - Wiley Online Library
Pollen and gene flow in fragmented habitats. Authors. *. Manja M. Kwak,. Corresponding author. *Laboratory of Plant Ecology and ... Wiley Online Library. *11. David M. Lowenstein, Kevin C. Matteson, Emily S. Minor, Diversity of wild bees supports pollination ... Shenhua Qian, Waka Saito, Makiko Mimura, Shingo Kaneko, Yuji Isagi, Eri Mizumachi, Akira S. Mori, Asymmetric gene flow and the ... In the absence of stepping stones even short distances between populations act as strong barriers for gene flow. ...
P[acman]-generated fruit fly gene 'library': A new research tool
... so that a researcher can choose a gene and find the corresponding clones in the library that cover that gene. Their ... "You can insert a single copy of a gene and rescue a mutation, or do a structure/function analysis of the gene," Bellen said. " ... P[acman]-generated fruit fly gene library: A new research tool. 26.05.2009 ... generated fruit fly gene , artificial chromosome , fruit fly , genetic research , genomic libraries , transgenesis ...
Eugene Field Curbside Holds Pickup | Denver Public Library
Specific Instructions for Eugene Field Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park in ... Specific Instructions for Eugene Field. Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park ... All Denver Public Library locations remain closed except for curbside holds pickup. New holds can now be placed. ... As per City ordinance, we ask all customers to wear face masks when visiting the library for curbside pickup. ...
Eugene Field Curbside Holds Pickup | Denver Public Library
Specific Instructions for Eugene Field Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park in ... Specific Instructions for Eugene Field. Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park ... Holds scheduled for curbside pickup will be available at the Eugene Field Branch Library. ... Picking Up: call when you arrive any time during your appointment slot, bring your library card or ID, dont forget your mask. ...
Even Gene Editors Need an Editor | Bioethics Research Library
Even Gene Editors Need an Editor. During the international conference that went by the hashtag #GeneEditSummit, a message on ... Science News reported that human gene editing was given "a green light. The Guardian agreed: "Summit rules out ban." "No ban, ... Take the official statement that concluded the International Summit on Human Gene Editing in Washington, D.C. (aka # ... determining appropriate use of emergent technology capable of altering genes in a way that might cure diseases such as HIV, ...
WorkflowSequenceRegulationSequencesPanelsNucleic acidHybridizationGenomeThousands of genesDrosophilaCatalog2020SearchNiobrara County LibraryGenomicTarget genesExpressionCollectionsApoptosisMammalianEmbryosRetroviralScientistsResearchersField Branch LibraryRNAiLuen YangSpecific geneCLONINGMOLECULARAntibodyMicroarray2000ComplexPlasmids2002HumanExperimentsCombinatorialPublic LibraryVivoSilencingSuccessfullyRegister-GuardAnnotationsCaenorhabditisAuthorEnsemblMechanismDiseasesCity CouncilWorld'sNewly discoveredManuscript LibraryTherapeutic1959Curbside pickup
- A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. (nih.gov)
- With the availability of complete genome sequences and the introduction of RNA-mediated gene interference (RNAi), systematic reverse genetic screens are now also possible. (nih.gov)
- Until now, such genome-wide RNAi screens have mostly been restricted to cultured cells and ubiquitous gene inactivation in Caenorhabditis elegans. (nih.gov)
- Here we report the generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact organism. (nih.gov)
- We generated 22,270 transgenic lines, covering 88% of the predicted protein-coding genes in the Drosophila genome. (nih.gov)
- They can now take a genome sequence (or many of them) and make a protein library for screening with unprecedented speed, cost-effectiveness and precision, allowing rapid discovery of potentially beneficial biomolecules from a genome. (eurekalert.org)
- Using a specially adapted tool called P[acman], a collaboration of researchers led by Baylor College of Medicine has established a library of clones that cover most of the genome of Drosophila melanogaster (fruit fly) and should speed the pace of genetic research. (innovations-report.com)
- Threadgill developed the idea for the CC in order to harness the power of so-called whole genome studies that examine all genes at once instead of subsets of genes. (healthcanal.com)
- refs) Vector-based siRNA delivery strategies for high-throughput screening of novel target genes Meihong Chen †, Quan Du ‡, Hong-Yan Zhang ‡, Claes Wahlestedt ‡§, and Zicai Liang ‡* † Chinese Human Genome Center North, Beijing, 100176, China and Institute. (libpubmedia.co.uk)
- The research team created a new genome-wide CRISPR library of 88,000 guide RNAs that enabled them to switch off each of the 20,000 genes from mouse Th2 cells. (b-s-h.org.uk)
- They mimicked an infection in cultured Th2 cells and looked at how switching off each single gene in the genome affected the activation or differentiation. (b-s-h.org.uk)
- Human COBLL1 genome location and COBLL1 gene details page in the UCSC Genome Browser. (wikipedia.org)
Thousands of genes4
- Scientists at Rutgers University-New Brunswick in collaboration with Johns Hopkins University and Harvard Medical School have invented a technology to clone thousands of genes simultaneously and create massive libraries of proteins from DNA samples, potentially ushering in a new era of functional genomics. (eurekalert.org)
- The DNA mciroarray gene data is in the expression levels of thousands of genes for a small amount of samples. (igi-global.com)
- Recent advances in microarray technology have enabled the measurement of the simultaneous expression of thousands of genes under multiple experimental conditions. (igi-global.com)
- It takes thousands of genes to build the photosynthetic machinery that plants need to harness sunlight for growth. (brightsurf.com)
- Our transgenic RNAi library thus opens up the prospect of systematically analysing gene functions in any tissue and at any stage of the Drosophila lifespan. (nih.gov)
- a split of the Hox cluster in a non- Drosophila insect," Development Genes and Evolution , vol. 214, no. 12, pp. 606-614, 2004. (hindawi.com)
- The following is a list of libraries from ProgrammableWeb's Library Directory that matched your search term. (programmableweb.com)
- Although there many different interpretations of the word "library" among software developers, ProgrammableWeb adheres to a specific definition so as to clearly distinguish libraries from SDKs and frameworks in a way that will facilitate clean search results. (programmableweb.com)
- We will discuss how to avoid doing a BLAST search and still obtain the relevant information and Blast Variations for structure/function search The Gene Expression Omnibus (GEO) is a public repository that archives and freely distributes microarray, next-generation sequencing, and other forms of high-throughput functional genomic data. (harvard.edu)
Niobrara County Library1
- It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences. (nih.gov)
- Lists of duplicated genes can be used to analyze the expression of genes from microarray profiling experiments, to characterize the genomic content of a specific chromosomal region, or to study the duplication status of a specific gene or group of genes. (programmableweb.com)
- Venken adapted the P[acman] vector to create genomic libraries, so that a researcher can choose a gene and find the corresponding clones in the library that cover that gene. (innovations-report.com)
- A genomic BAC library and a new BAC-GFP vector to study the holocentric pest Spodoptera frugiperda ," Insect Biochemistry and Molecular Biology , vol. 34, no. 4, pp. 331-341, 2004. (hindawi.com)
- Highly conserved gene order and numerous novel repetitive elements in genomic regions linked to wing pattern variation in Heliconius butterflies," BMC Genomics , vol. 9, article 345, 2008. (hindawi.com)
- Efficient Retrieval Technique for Microarray Gene Expression. (igi-global.com)
- Microarray techniques have been successfully used to investigate useful information for cancer diagnosis at the gene expression level, the true integration of existing methods into day-to-day clinical practice is very challenging. (igi-global.com)
- Mammalian expression cloning methods that permit multiple rounds of selection and enrichment have been extremely successful in identifying cDNA clones from plasmid libraries that correspond to unknown receptors or ligands ( 7 ). (pnas.org)
- Unfortunately, the requirement for large T antigen-driven replication of plasmid libraries in mammalian cells has severely limited adaptation of expression cloning methods to functional screens with only highly transfectable cell lines. (pnas.org)
- The regulation of P450 gene expression has been well documented in experimental animals, but at present there is very little information available about the regulation of human P450 genes, particularly in extra-hepatic tissues. (bl.uk)
- Regulation of P450 expression by a range of xenobiotics, known to have profound effects on the expression of rodent P450 genes, has been studied in a mouse model and in cultured cells. (bl.uk)
- TCPOBOP was shown to be equally effective at influencing human P450 gene expression and, in most cases, the patterns of gene regulation observed in experimental animals were also seen in the human tumours. (bl.uk)
- Why do you need gene expression library by the way? (helico.com)
- Construction of the SPL was performed in a vector (pSIP409) previously developed for high-level, inducible gene expression in L. plantarum and Lactobacillus sakei. (nofima.no)
- The SPIL enables fine-tuning of stable gene expression for various applications in L. plantarum. (nofima.no)
- Regulation of aldose reductase gene expression in renal cortex and medulla of rats. (bl.uk)
- Non-parallelism of islet amyloid polypeptide (amylin) and insulin gene expression in rats islets following dexamethasone treatment. (bl.uk)
- refs) PCR-based expression analysis and identification of microRNAs David P Lu †, Rebecca L Read †, David T Humphreys ‡, Fiona M Battah †, David I K Martin ‡ and John E J Rasko †§* † Gene. (libpubmedia.co.uk)
- Second, deregulation of the expression of septin family genes in hematological cancers can be observed either with or without the concomitant presence of MLL gene fusions. (begellhouse.com)
- The fonds was donated to University of Manitoba Archives & Special Collections by Gene Telpner in 1991. (umanitoba.ca)
- The Library of Congress maintains one of the largest and most diverse collections of scientific and technical information in the world. (loc.gov)
- The Science, Technology and Business Division provides reference and bibliographic services and develops the general collections of the Library in all areas of science, technology, business and economics, with the exception of clinical medicine and technical agriculture, which are the subject specialties of the National Library of Medicine and the National Agricultural Library. (loc.gov)
- Human and mouse clone collections are compiled into libraries for gene families and pathways. (horizondiscovery.com)
- Gene Ontology (GO) annotated collections targeting all genes in a particular gene family or biological pathway have been created from the human TRC lentiviral shRNA library. (horizondiscovery.com)
- Three of the genes, RelA, cellular FLICE-like inhibitory protein (c-FLIP), and a dominant-negative mutant of TNF receptor 1 arising through CPR afforded strong protection against apoptosis. (pnas.org)
- Two of the genes identified, Dbs and Fas-associated death domain protein (FADD), previously identified as a proapoptotic molecule, afforded partial protection against TNFα-induced apoptosis. (pnas.org)
- The proposed trial combines recent advances in cancer therapy -- spearheaded by Steven A. Rosenberg at the National Cancer Institute -- with work by National Heart, Blood and Lung Institute researcher W. French Anderson, a pioneer in the art of inserting foreign genes into mammalian cells. (thefreelibrary.com)
- Genes regulating responses in mammalian cells are often difficult to identify by functional cloning strategies limited to a single round of selection. (pnas.org)
- Retroviral cDNA libraries have been used in a number of functional screens to identify unique mammalian genes that regulate cellular responses ( 1 - 6 ). (pnas.org)
- To ensure that positive cDNA clones are successfully isolated from complex libraries in a single round of selection, functional screens using mammalian cells have been restricted to cellular assays in which the background of false-positive cells meeting a selection criterion is low, typically 1 in 10 5 false-positive cells. (pnas.org)
- Here we describe an alternative method called cyclical packaging rescue (CPR) that uses direct repackaging of retroviral RNAs into new infectious virions to identify genes regulating functional responses in mammalian cells (Fig. 1 A ). In CPR, stably integrated helper-free retroviral libraries are recovered rapidly from mammalian cells as infectious helper-free retroviral supernatants 24 h after infection with adenoviruses expressing retroviral gag-pol and env genes. (pnas.org)
- These results suggest that CPR is a rapid and versatile approach that should facilitate functional analysis of gene function in mammalian cells. (pnas.org)
- Sry is believed to act as a switch in the complex gene mechanism that diverts mammalian development from its usual course - the female - to the male. (sciencephoto.com)
- Here we describe a strategy, cyclical packaging rescue (CPR), which allows rapid recovery and retransmission of retroviral cDNA libraries. (pnas.org)
- When performed in concert with selection using functional assays, cDNAs regulating functional responses can be identified by enrichment through multiple rounds of retroviral library recovery and retransmission. (pnas.org)
- Scientists have created the first retroviral CRISPR-Cas9 gene editing library to examine the regulation of mouse T cells, it was announced last week. (b-s-h.org.uk)
- Dr Xi Chen, joint first author from the Wellcome Sanger Institute, said while previous studies have mostly examined one gene at time, the development of a retroviral CRISPR library has enabled them to knock-out each individual gene in mouse T helper cells for the first time. (b-s-h.org.uk)
- Moreover, the initial 30-day culture period will give scientists a chance to test the gene-altered cells for unusual changes or vira contaminants -- concerns that have been unresolved in previous gene therapy proposals -- before injecting them into patients. (thefreelibrary.com)
- Researchers working in fields such as protein engineering, discovery biology, structural biology, synthetic biology, antibody engineering, and enzyme engineering choose GENEWIZ's Synthetic DNA Libraries because of our wide range of library types, reliable service, and best-in-class consultation from our Ph.D.-level customer support scientists. (genewiz.com)
- With competitive pricing and project completion in as little as 11 days, discover why hundreds of scientists from leading research facilities choose GENEWIZ for their partially (NNK) or completely randomized (NNN) nucleotide libraries. (genewiz.com)
- Moving beyond some of the alarming sci-fi scenarios of gene editing, groundbreaking scientists are harnessing the power of these biological breakthroughs to save lives. (lfla.org)
- With methods developed by Anderson, researchers can use a retrovirus to insert into TIL cells a gene that confers resistance to the antibiotic neomycin. (thefreelibrary.com)
- The researchers reported in the Jan. 15 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES that this inheritance pattern suggests that the ATHS gene lies on chromosome 19, near the gene for the LDL receptor. (thefreelibrary.com)
- In 15 papers published Feb. 16 in the Genetics Society of America journals Genetics and G3:Genes/Genomes/Genetics , researchers from North Carolina State University, the University of North Carolina at Chapel Hill, The Jackson Laboratory and other universities and labs across the globe highlight a new genetic resource that could aid development of more effective treatments for any number of human diseases. (healthcanal.com)
- Learning more about these interactions could help researchers tease out links between certain genes and certain diseases, for example. (healthcanal.com)
- And yet, researchers don't know exactly how these genes work. (brightsurf.com)
- Now a team led by Princeton University researchers has constructed a public "library" to help researchers to find out what each gene does. (brightsurf.com)
- Unlocking the role of each gene could allow researchers to engineer plants to grow more quickly, potentially meeting future world food needs. (brightsurf.com)
- The ability to observe a Chlamy cell with just one defective gene among all the other functioning genes allows researchers to figure out what that gene does. (brightsurf.com)
- The library enables researchers to test multiple mutant Chlamy strains at once because each mutation is labeled with a unique "DNA barcode. (brightsurf.com)
- We have partnered with the TRC to make the shRNA libraries, which are available to researchers worldwide, and to compile shRNA libraries for popular gene families and pathways. (horizondiscovery.com)
- We developed a prototype functional alerting system for researchers based on the GeneRIFs, and a strategy to find all of the literature related to genes. (nih.gov)
Field Branch Library1
- Our RNAi transgenes consist of short gene fragments cloned as inverted repeats and expressed using the binary GAL4/UAS system. (nih.gov)
- RNAi2011: Gene Regulation by Small RNAs, the 6 th international Oxford RNAi conference, was held at St Hilda's College, University of Oxford (UK), between 29 and 31 March, 2011. (libpubmedia.co.uk)
- A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. (horizondiscovery.com)
- Lentivirus-delivered stable gene silencing by RNAi in primary cells. (horizondiscovery.com)
- For this study, we attempted to produce dsRNA of the chitin deacetylase (CDA) region of the lgx-1 gene in vitro to be used for RNAi experiments by soaking. (tufts.edu)
- Discovering the function of a gene requires cloning a DNA sequence and expressing it. (eurekalert.org)
- The team also reported the capture and cloning of the first protein library, or suite of proteins, from a human microbiome sample. (eurekalert.org)
- The next phase, which is underway, is to improve the cloning process, build libraries and discover therapeutic proteins found in our genomes, Parekkadan said. (eurekalert.org)
- Molecular cloning of a group of mouse pancreatic islet beta-cell-related genes by random cDNA sequencing. (bl.uk)
- The T47D cell ds cDNA was generated by SMART TM technology and the library was constructed using in vivo recombination-mediated cloning in the AH109 yeast strain using a pGADT7-Rec plasmid. (go.jp)
- In a report in the current online issue of the journal Nature Methods, Dr. Hugo Bellen (http://flypush.imgen.bcm.tmc.edu/lab/), a professor of molecular and human genetics at BCM and a Howard Hughes Medical Institute investigator, and his colleagues describe the new libraries. (innovations-report.com)
- Molecular model of catabolite gene activator protein (CAP, pink and green) bound to a molecule of deoxyribonucleic acid (DNA, across top). (sciencephoto.com)
- Isogenica is currently commercialising the Colibra™ technology for ratio controlled protein and antibody libraries based on a partly automated process. (businesswire.com)
- Isogenica's CIS display technology is an in vitro display technology that allows the rapid generation of polypeptide and antibody libraries from which it is possible to select lead molecules with high affinity and specificity for most targets. (businesswire.com)
- From the microarray gene data, the process of extracting the required knowledge remains an open challenge. (igi-global.com)
- The technique like LPP and PCA is chosen for the dimensionality reduction for efficient retrieval of microarray gene data. (igi-global.com)
- An application of microarray gene data is included with classification by SVM. (igi-global.com)
- Complex interactions between large numbers of genes frequently govern traits and behavior. (healthcanal.com)
- Published online in the journal Cell , the work focuses on the complex control mechanism, describing how different genes are involved in both activation and development of T helper cells. (b-s-h.org.uk)
- To investigate the interaction factors of C35 in its high expressed breast cancer cell lines, we constructed bait recombinant plasmids of C35 gene and T47D cell cDNA library for yeast two-hybrid screening. (go.jp)
- The three kinds of C35 genes were successfully amplified and inserted into pGBKT7 to construct bait recombinant plasmids pGBKT7-C351-348bp, pGBKT7-C351-153bp and pGBKT7-C35154-348bp, then transformed into Y187 yeast cells by the lithium acetate method. (go.jp)
- Human gene-splice test considered. (thefreelibrary.com)
- Experiments in animals suggest such "gene therapy" may prove useful against thousands of hereditary diseases, but a variety of medical and ethical concerns have stalled the start of human experiments. (thefreelibrary.com)
- Dr. Kathy Niakan, the researcher who will be performing the experiments, said, "We would really like to understand the genes needed for a human embryo to develop successfully into a healthy baby. (georgetown.edu)
- Take the official statemen t that concluded the International Summit on Human Gene Editing in Washington, D.C. (aka #GeneEditSummit). (georgetown.edu)
- Science News reported that human gene editing was given "a green light. (georgetown.edu)
- In scientific publications, the libraries should be referred to as TRC-Hs1.0 (Human) and TRC-Mm1.0 (Mouse). (horizondiscovery.com)
- 60% of these were associated with human genes and 27% with mouse genes. (nih.gov)
- This highly validated library includes nearly 1800 individual siRNAs targeting 597 human kinases. (thermofisher.com)
- The leaps and advances of science and technology to revolutionize human DNA have sparked fierce public debate about what the future of gene editing holds for humanity. (lfla.org)
- The lgx-1 gene in C. elegans codes for a putative chitin deacetylase and has been shown to be expressed in the pharynx of the organism by in situ hybridization experiments (Kohara, 2010). (tufts.edu)
- Here is an example of the power of Ambion's Silencer™ Kinase siRNA Library, as demonstrated through preliminary screening experiments carried out by Cenix BioScience GmbH. (thermofisher.com)
- This will allow both parties to efficiently optimise the assembly of genes from smaller DNA fragments and generate combinatorial libraries of genetic mutations for application in the field of synthetic biology. (businesswire.com)
- Learn how the trimer-controlled combinatorial DNA library synthesis approach provides full control over amino acid distribution and offers higher efficiency for downstream screening. (genewiz.com)
- A combinatorial library allows you to create a library of mutants by using different genetic elements in multiple combinations to determine the most beneficial combination. (genewiz.com)
- You can obtain a combinatorial library using traditional partially randomized (NNK/NNS) or completely randomized (NNN) approaches. (genewiz.com)
- The Eugene Public Library is a municipal public library in Eugene, Oregon, United States. (wikipedia.org)
- Eugene Public Library was established as a tax-supported entity in 1904. (wikipedia.org)
- Eugene Public Library 25 years old Thursday. (wikipedia.org)
- All Denver Public Library locations remain closed except for curbside holds pickup. (denverlibrary.org)
- In this case, the Eugene Public Library Foundation has pledged to make the program possible and completely free for every child living within Eugene city limits. (eugeneweekly.com)
- The online signup opened Sept. 5, and since then 1,473 of the approximately 8,000 eligible Eugene children have registered for the program, which Eugene Public Library Customer Experience Manager LaVena Nohrenberg says was "around where the Imagination Library said we'd be in 18 months. (eugeneweekly.com)
- Kids and families can Explore, discover, and create together during STEAM activities every Wednesday at 4PM at the Sheldon Branch of the Eugene Public Library, and every Thursday at 4PM at the Bethel Branch. (krvm.org)
- The Bethel Branch of Eugene Public Library is located at 1990 Echo Hollow Road. (krvm.org)
- The Springfield Public Library and the Springfield Museum will be closed through May 31. (registerguard.com)
- On Monday, the Springfield Public Library announced the extended closure on its Twitter page. (registerguard.com)
- The Eugene Public library is hosting a Small Business Clinic from 5 to 7PM on Thursday January 4 and Thursday, January 18 at the Downtown Branch. (krvm.org)
- This project is provided by Eugene Public Library and SCORE: Counselors to America's Small Business. (krvm.org)
- In the midst of the city's budget crisis, the Library Journal rated Eugene Public Library a three-star library. (eugeneweekly.com)
- Dr. Eugene Clark Library is a Public library. (librarytechnology.org)
- Join a community of Los Angeles Public Library supporters. (lfla.org)
- Enriching the programs of the Los Angeles Public Library. (lfla.org)
- All proceeds benefit the Los Angeles Public Library. (lfla.org)
- Public Library Asso. (wisconsinhistory.org)
- Kostas Kostarelos (University of London, UK) described how carbon nanotubes, which are internalised by the cell without apparent damage to cellular structure, could be chemically functionalised and combined with siRNAs to form gene-silencing complexes that are effectively delivered to target cells. (libpubmedia.co.uk)
- mRNA Silencing by 178 Kinase siRNAs from the Silencer ™ Kinase siRNA Library. (thermofisher.com)
- The exhibition, which includes manuscripts, correspondence, limited editions and other materials from the private collection of Frank J. Piehl (Ph.D., 1952) and the holdings of the University of Chicago Library, focuses on Field as author, collector and promoter of books and the book arts. (uchicago.edu)
- In one of the 15 papers, Threadgill and corresponding author Dr. Francis S. Collins, director of the National Institutes of Health, identify key genes involved in red and white blood cell counts and red blood cell volume. (healthcanal.com)
- Xiaobo Li, the study's first author, was a postdoctoral researcher at Princeton when the team completed the library. (brightsurf.com)
- Contributors: Gene H. Bell-Villada - Author. (questia.com)
- Baltimore deserves praise him for orchestrating an important three-day conversation that was truly international on a morally, culturally divisive issue - determining appropriate use of emergent technology capable of altering genes in a way that might cure diseases such as HIV, hepatitis-B and sickle cell anemia. (georgetown.edu)
- As the holiday season approaches, the Eugene City Council was feeling generous at its Nov. 23 meeting. (eugeneweekly.com)
- In 2005, the Eugene City Council adopted and applied the /WR Water Resources Conservation Overlay Zone measures to implement statewide planning Goal 5 inside Eugene's city limits. (eugene-or.gov)
- Although the current proposal simply calls for inserting a marker gene, later proposals may seek approval for the insertion into TIL cells of genes that code for the production of potentially therapeutic factors such as interleukin-2 or tumor necrosis factor, Blaese says. (thefreelibrary.com)
- T. Yamamoto, Y. Tsunetsugu-Yokota, Prospects for the therapeutic application of lentivirus-based gene therapy to HIV-1 infection. (horizondiscovery.com)