Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Amino Acid Sequence
Combinatorial Chemistry Techniques
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Directed Molecular Evolution
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
A phylum of bacteria consisting of the purple bacteria and their relatives which form a branch of the eubacterial tree. This group of predominantly gram-negative bacteria is classified based on homology of equivalent nucleotide sequences of 16S ribosomal RNA or by hybridization of ribosomal RNA or DNA with 16S and 23S ribosomal RNA.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
National Library of Medicine (U.S.)
An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Sequence Homology, Nucleic Acid
Sequence Homology, Amino Acid
One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.
Small Molecule Libraries
Genetic Complementation Test
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Library Collection Development
Development of a library collection, including the determination and coordination of selection policy, assessment of needs of users and potential users, collection use studies, collection evaluation, identification of collection needs, selection of materials, planning for resource sharing, collection maintenance and weeding, and budgeting.
A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)
Polymorphism, Restriction Fragment Length
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Library Technical Services
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).
Gene Expression Regulation, Bacterial
Facility Design and Construction
Architecture, exterior and interior design, and construction of facilities other than hospitals, e.g., dental schools, medical schools, ambulatory care clinics, and specified units of health care facilities. The concept also includes architecture, design, and construction of specialized contained, controlled, or closed research environments including those of space labs and stations.
Specialists in the management of a library or the services rendered by a library, bringing professional skills to administration, organization of material and personnel, interpretation of bibliothecal rules, the development and maintenance of the library's collection, and the provision of information services.
Chromosomes, Artificial, Bacterial
A computerized biomedical bibliographic storage and retrieval system operated by the NATIONAL LIBRARY OF MEDICINE. MEDLARS stands for Medical Literature Analysis and Retrieval System, which was first introduced in 1964 and evolved into an online system in 1971 called MEDLINE (MEDLARS Online). As other online databases were developed, MEDLARS became the name of the entire NLM information system while MEDLINE became the name of the premier database. MEDLARS was used to produce the former printed Cumulated Index Medicus, and the printed monthly Index Medicus, until that publication ceased in December 2004.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
High-Throughput Screening Assays
Architecture as Topic
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Interior Design and Furnishings
Bibliography as Topic
Discussion of lists of works, documents or other publications, usually with some relationship between them, e.g., by a given author, on a given subject, or published in a given place, and differing from a catalog in that its contents are restricted to holdings of a single collection, library, or group of libraries. (from The ALA Glossary of Library and Information Science, 1983)
Two-Hybrid System Techniques
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Gene Expression Profiling
Drug Evaluation, Preclinical
Open Reading Frames
Sequence Tagged Sites
Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.
Cell Surface Display Techniques
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
High-Throughput Nucleotide Sequencing
Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.
The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Recombinant Fusion Proteins
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Extensive collections, reputedly complete, of references and citations to books, articles, publications, etc., generally on a single subject or specialized subject area. Databases can operate through automated files, libraries, or computer disks. The concept should be differentiated from DATABASES, FACTUAL which is used for collections of data and facts apart from bibliographic references to them.
The Genexpress IMAGE knowledge base of the human brain transcriptome: a prototype integrated resource for functional and computational genomics. (1/9359)Expression profiles of 5058 human gene transcripts represented by an array of 7451 clones from the first IMAGE Consortium cDNA library from infant brain have been collected by semiquantitative hybridization of the array with complex probes derived by reverse transcription of mRNA from brain and five other human tissues. Twenty-one percent of the clones corresponded to transcripts that could be classified in general categories of low, moderate, or high abundance. These expression profiles were integrated with cDNA clone and sequence clustering and gene mapping information from an upgraded version of the Genexpress Index. For seven gene transcripts found to be transcribed preferentially or specifically in brain, the expression profiles were confirmed by Northern blot analyses of mRNA from eight adult and four fetal tissues, and 15 distinct regions of brain. In four instances, further documentation of the sites of expression was obtained by in situ hybridization of rat-brain tissue sections. A systematic effort was undertaken to further integrate available cytogenetic, genetic, physical, and genic map informations through radiation-hybrid mapping to provide a unique validated map location for each of these genes in relation to the disease map. The resulting Genexpress IMAGE Knowledge Base is illustrated by five examples presented in the printed article with additional data available on a dedicated Web site at the address http://idefix.upr420.vjf.cnrs.fr/EXPR++ +/ welcome.html. (+info)
Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (2/9359)Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space. (+info)
Cloning of a bovine orphan transporter and its short splicing variant. (3/9359)We have isolated a cDNA (bv7-3) encoding a member of the Na+,Cl(-)-dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining. (+info)
The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution. (4/9359)Latrophilin is a brain-specific Ca2+-independent receptor of alpha-latrotoxin, a potent presynaptic neurotoxin. We now report the finding of two novel latrophilin homologues. All three latrophilins are unusual G protein-coupled receptors. They exhibit strong similarities within their lectin, olfactomedin and transmembrane domains but possess variable C-termini. Latrophilins have up to seven sites of alternative splicing; some splice variants contain an altered third cytoplasmic loop or a truncated cytoplasmic tail. Only latrophilin-1 binds alpha-latrotoxin; it is abundant in brain and is present in endocrine cells. Latrophilin-3 is also brain-specific, whereas latrophilin-2 is ubiquitous. Together, latrophilins form a novel family of heterogeneous G protein-coupled receptors with distinct tissue distribution and functions. (+info)
Identification of the human melanoma-associated chondroitin sulfate proteoglycan antigen epitope recognized by the antitumor monoclonal antibody 763.74 from a peptide phage library. (5/9359)To identify the epitope of the melanoma-associated chondroitin sulfate proteoglycan (MCSP) recognized by the monoclonal antibody (mAb) 763.74, we first expressed random DNA fragments obtained from the complete coding sequence of the MCSP core glycoproteins in phages and selected without success for binders to the murine mAb 763.74. We then used a library of random heptapeptides displayed at the surface of the filamentous M13 phage as fusion protein to the NH2-terminal portion of the minor coat protein III. After three rounds of selection on the bound mAb, several phages displaying related binding peptides were identified, yielding the consensus sequence Val-His-Leu-Asn-Tyr-Glu-His. Competitive ELISA experiments showed that this peptide can be specifically prevented from binding to mAb 763.74 by an anti-idiotypic MK2-23 mouse:human chimeric mAb and by A375 melanoma cells expressing the antigen MCSP. We screened the amino acid sequence of the MCSP molecule for a region of homology to the consensus sequence and found that the amino acid sequence Val-His-Ile-Asn-Ala-His spanning positions 289 and 294 has high homology. Synthetic linear peptides corresponding to the consensus sequence as well as to the MCSP-derived epitope inhibit the binding of mAb 763.74 to the phages displaying the consensus amino acid sequence. Finally, the biotinylated consensus peptide absorbed to streptavidin-microtiter plates can be used for the detection of mAb 763.74 in human serum. These results show clearly that the MCSP epitope defined by mAb 763.74 has been identified. (+info)
Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network. (6/9359)SNARE proteins are known to play a role in regulating intracellular protein transport between donor and target membranes. This docking and fusion process involves the interaction of specific vesicle-SNAREs (e.g. VAMP) with specific cognate target-SNAREs (e.g. syntaxin and SNAP-23). Using human SNAP-23 as the bait in a yeast two-hybrid screen of a human B-lymphocyte cDNA library, we have identified the 287-amino-acid SNARE protein syntaxin 11. Like other syntaxin family members, syntaxin 11 binds to the SNARE proteins VAMP and SNAP-23 in vitro and also exists in a complex with SNAP-23 in transfected HeLa cells and in native human B lymphocytes. Unlike other syntaxin family members, no obvious transmembrane domain is present in syntaxin 11. Nevertheless, syntaxin 11 is predominantly membrane-associated and colocalizes with the mannose 6-phosphate receptor on late endosomes and the trans-Golgi network. These data suggest that syntaxin 11 is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network in mammalian cells. (+info)
Caffeine can override the S-M checkpoint in fission yeast. (7/9359)The replication checkpoint (or 'S-M checkpoint') control prevents progression into mitosis when DNA replication is incomplete. Caffeine has been known for some time to have the capacity to override the S-M checkpoint in animal cells. We show here that caffeine also disrupts the S-M checkpoint in the fission yeast Schizosaccharomyces pombe. By contrast, no comparable effects of caffeine on the S. pombe DNA damage checkpoint were seen. S. pombe cells arrested in early S phase and then exposed to caffeine lost viability rapidly as they attempted to enter mitosis, which was accompanied by tyrosine dephosphorylation of Cdc2. Despite this, the caffeine-induced loss of viability was not blocked in a temperature-sensitive cdc2 mutant incubated at the restrictive temperature, although catastrophic mitosis was prevented under these conditions. This suggests that, in addition to S-M checkpoint control, a caffeine-sensitive function may be important for maintenance of cell viability during S phase arrest. The lethality of a combination of caffeine with the DNA replication inhibitor hydroxyurea was suppressed by overexpression of Cds1 or Chk1, protein kinases previously implicated in S-M checkpoint control and recovery from S phase arrest. In addition, the same combination of drugs was specifically tolerated in cells overexpressing either of two novel S. pombe genes isolated in a cDNA library screen. These findings should allow further molecular investigation of the regulation of S phase arrest, and may provide a useful system with which to identify novel drugs that specifically abrogate the checkpoint control. (+info)
Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (8/9359)A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided. (+info)
Subtractive cDNA Library Construction Service - Creative Biogene
Creative Biogene is a biotechnology company which has the expertise and experience to provide you with high quality subtractive cDNA library construction services.
Frontiers | Evaluation of Rapid Library Preparation Protocols for Whole Genome Sequencing Based Outbreak Investigation | Public...
Whole genome sequencing (WGS) has become the new gold standard for bacterial outbreak investigation, due to the high resolution available for typing. While sequencing is currently predominantly performed on Illumina devices, the preceding library preparation can be performed using various protocols. Enzymatic fragmentation library preparation protocols are fast, have minimal hands-on time, and work with small quantities of DNA. The aim of our study was to compare three library preparation protocols for molecular typing: Nextera XT (Illumina); Nextera Flex (Illumina); and QIAseq FX (Qiagen). We selected 12 ATCC strains from human Gram-positive and Gram-negative pathogens with %G+C-content ranging from 27% (Fusobacterium nucleatum) to 73% (Micrococcus luteus), each having a high quality complete genome assembly available, to allow in-depth analysis of the resulting Illumina sequence data quality. Additionally, we selected isolates from previously analyzed cases of vancomycin-resistant Enterococcus faecium
Cdna Library Construction And Screening Pdf, Session Activities > luciegaillard...
Cdna library construction and screening pdf The sense of an ending book pdf, Construction and screening of a genomic library specific for mouse chromosome isolation ofdermed genetic locias well as form the basis for the production of.
Illumina Sequencing Library Construction from ChIP DNA
The Illumina sequencing platform is very popular among next-generation sequencing platforms. However, the DNA sequencing library construction kit provided by Illumina is considerably expensive. The protocol described here can be used to construct high-quality sequencing libraries from chromatin immunoprecipitated DNA. It uses key reagents from third-party vendors and greatly reduces the cost in library construction for Illumina sequencing.
low yield in library preparation: post #1
low yield in library preparation - posted in ChIP and Next Generation Sequencing: I got a very successful ChIP (DNA sheared to enrichment around 200 bp, more than 2% input at positive controls). Then I used 10 ng ChIPed DNA to make library for sequencing with the NEB kit. But I got a very poor yield (0.4 ng/ul in 30 ul elutes). I repeated twice with the same DNA and the results didnt turn any better. I used the same kit to make another library two months ago and it worked pretty fine....
cDNA Library Construction Kits | Thermo Fisher Scientific
Construct high quality full length cDNA libraries using SuperScript® reverse transcriptase combined with Gateway® cloning for uncut libraries (no use of restriction digestion). Or use standard restriction enzyme cloning.
SeqOnce Biosciences RhinoSeq Rapid DNA Library Preparation Kit | GenomeWeb
SeqOnce Biosciences has launched its RhinoSeq DNA Library Preparation Kit for next-generation sequencing sample preparation. The firm said that the five tube kit contains preformatted master mixes for a simple, fast, and stable workflow. The tool generates a temporary structure that creates sequence-specific single stranded overhangs for sequencing adaptor litigation. When
SPRIselect, Size Selection for Library Preparation - Beckman Coulter
Beckman Coulters SPRIselect is a SPRI-based chemistry that speeds & simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.
OGT Expands Product Portfolio with Complete Library Preparation Solution
OGT has announced the expansion of its SureSeq portfolio with a complete library preparation solution for hybridization-based target capture in NGS.
New Method to Directly Sequence Small Genomes Without Library Preparation - ECA Academy
For the first time, researchers sequenced DNA molecules without the need for the standard pre-sequencing workflow known as library preparation. Read more here.
western blot test - Sebov detection kits
A Survey of Virus Recombination Uncovers Canonical Choices of Artificial Chimeras Generated All through Deep Sequencing Library Preparation. Chimeric reads shall be generated by in vitro recombination all through the preparation of high-throughput sequencing libraries. Our attempt to detect natural recombination between the genomes of dengue virus (DENV; +ssRNA genome) and its mosquito host using the Illumina Nextera sequencing library preparation bundle revealed that almost all, if not all, detected host-virus chimeras had […]. ...
Illumina Introduces Automated Library Preparation - Bio-IT World
By Bio-IT World Staff. February 23, 2015 , This morning, DNA sequencing company Illumina released a new product, NeoPrep, to work alongside its line of next-generation sequencers. NeoPrep is a benchtop device that automatically prepares a DNA or RNA sample for sequencing on Illumina instruments, usually a time-consuming manual process that involves shearing the sample into short fragments, repairing the fragment ends, and linking them to Illumina-specific adapter sequences to create a library of size-selected DNA molecules. Illuminas new instrumentation allows this work to be performed within the NeoPrep device, on a microfluidic cartridge called a NeoPrep library card, in which up to 16 samples in parallel can be processed through each step of the library prep process. While creating libraries will still take between four and eight hours using NeoPrep, lab technicians only have to be present at the very beginning and end of the process, allowing overnight runs and freeing scientists to spend ...
Whole genome sequencing libraries in under 4 hours
Generate whole genome sequencing libraries from isolated single animal or bacterial cells or low amounts of genomic DNA in under 4 hours
RhinoSeq ABRF Poster 2019 - A 12-Min, Single Tube, Nanogram Input, Library Prep - SeqOnce
Current next Generation Sequencing (NGS) library construction is a bottleneck in the sequencing workflow. The processes of library construction for DNA and RNA sequencing are complex, error prone, costly, and time consuming. Current commercial products are multistep, multi-hour sample processing that tend to be cost and labor prohibitive. These issues have created a demand for a simple, rapid, and cost-effective library construction product that offers users application flexibility while minimizing construction complexity for low to ultra-high throughput sample processing. SeqOnce has developed a novel library technology that is rapid and minimizes sample processing complexity. The five tube RhinoSeq kit is comprised of formatted master mixes for a simple and stable user workflow. The 12 minute library construction uses a single master-mix that when combined with fragmentation and PCR steps produces libraries in less than 50 minutes. A size selection step occurs after PCR and the PCR free ...
To the Editor:. Recent studies have demonstrated that the fragment size of circulating tumor DNA (ctDNA) is shorter than normal cell-free DNA (cfDNA)1 (1, 2). As the most common ctDNA fragment lengths were between 134 bp and 144 bp, whereas the modal cfDNA fragment length was 167 bp (2), it was suggested that the enrichment of shorter plasma fragments might improve analysis of ctDNA. Recently, improved recovery of short plasma DNA fragments by single-stranded DNA (ssDNA) library preparation was reported (3). Therefore, we tested whether ssDNA library preparation results in an enrichment of ctDNA compared to the typically employed double-stranded DNA (dsDNA) libraries.. To this end, we applied an adaptor-ligation method as published by Burnham et al. (3) and prepared ssDNA and dsDNA libraries from 6 plasma samples derived from patients with breast (B7_2, B13_1), colon (C177_2, C177_3), and prostate (P226_4, P226_6), all with late-stage metastatic disease.. We first tested the fragment length ...
help - Molecular Cloning
Weve construct the cDNA library by using CloneMiner cDNA Library Construction Kit (Invitrogen). After the construction, were able to obtain high number of titers. Then, we decide to further the analysis by doing express sequence tag (EST). Anyway, were having problem to get the plasmid. We didnt get any plasmid after extraction. The LB broth, LB agar and antibiotic which is kanamycin are in good condition. Theres some situation, that even the bacteria are having problem to grow in the LB broth. Do you have any suggestion? At the moment, I dont have any ideas how to troubleshoot this problem. And my friends also didnt have any clue as this problem is quite strange to us.. ...
Improved genome sequencing using an engineered transposase | BMC Biotechnology | Full Text
Library construction plays an important role for high-throughput next generation sequencing (NGS). Plethora of library construction methods have been developed in the past few years [1-3]. Most common library preparation methods follow a basic procedure with minor variations . This procedure is usually lengthy and includes several steps. First, DNA is fragmented by sonication, nebulization or shearing to desired sizes. The fragmented DNA is then repaired and end-polished including blunt-end and A-tailing. Finally, platform-specific adaptors are ligated to DNA library [2, 4]. Typically, this process results in significant sample loss, and therefore, requires a DNA input amount of over 200 ng, sometimes up to 1 ug. This workflow also limits the throughput. Nextera, developed by Epicentre (an Illumina company), is an alternative approach to streamline the workflow, improve turnaround time and reduce DNA input and increase throughput. Nextera takes advantages of an in vitro transposition ...
genomic dna library screening
In prokaryotes, the structural genes coding for proteins are continuous … In order to isolate clones that contain regions of interest from a library, the library must first be screened. Gene Tagging 7. In order to construct a genomic library, the organisms DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. To understand what a recombinant genomic DNA library is and how it is constructed. Want To Turn Your Gaming Side Hustle Into A Career? A genomic library is a set of clones that together represents the entire genome of a given organism. This method aims at identifying the protein product of a cloned gene. Using genomic library screening, we identified two genes involved in propionate tolerance in Yarrowia lipolytica-MFS1 and RTS1. This is the screen-ing of a library with a labelled probe (ra-dioactive, bioluminescent, etc.) Once a genomic library is produced, researchers can work with it in a number of different ways. ...
Reverse Transcription Technology
There are three choices of RT primer: oligo(dT), random primers, or a gene-specific primer. The priming strategy has a strong impact on the workflow applied, since each priming method has prerequisites and consequences.. Oligo(dT) priming is used to receive full-length copies of the mRNA. For cDNA library construction or cDNA labeling applications, oligo(dT) primers are almost always used to prime cDNA synthesis. Anchored oligo(dT)18 primers are designed to bind at the beginning of the poly(A) tail (rather than randomly within the often long tail) to generate full-length cDNAs. This avoids mispriming and unnecessary reverse transcription of the long poly(A) tail. Since the 5´ ends of long mRNAs are often underrepresented in cDNA mixtures, this primer is preferred for most applications.. However, if the message is long or does not have a poly(A) tail (as with prokaryotic mRNA), then random hexamer primers are used. Random hexamer primers bind throughout the entire length of RNA, ensuring reverse ...
best polyA+ from total RNA method??
In article ,Pine.DYN.3.91.950209140740.15540A-100000 at uxa.cso.uiuc.edu, hodges bradley l ,segdoh at uxa.cso.uiuc.edu, writes: ,From: hodges bradley l ,segdoh at uxa.cso.uiuc.edu, ,Subject: Re: best polyA+ from total RNA method?? ,Date: Thu, 9 Feb 1995 14:09:10 -0600 ,Qiagen sells dT conjugated glass beads (Ithink). , ,On 1 Feb 1995 neale at mbcf.stjude.org wrote: ,, I was wondering if anyone had a strong recommendation of method for ,, purification of poly A+ RNA from total RNA. The samples of total RNA that we ,, have are about 300 ug each. We could pool some if necessary. ,, ,, We could purify poly A+ directly from cells if that is thought superior to ,, purifying from total RNA. Its just that we already have the total RNA, and ,, know that our gene of interest is expressed in these samples. ,, ,, We wish to use the poly A+ RNA for cDNA library construction. Its been a while ,, (5 years) since Ive had to do this and so I thought there may be an improved ,, method for selection of poly A+ ...
SMB DH5α Competent Cells [DH5α] | star-moon
產品說明:The most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. Carries recA1 mutation that eliminates homologous recombination ensuring insert stability. Carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. Useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb). GenotypeF¯ endA1 hsdR17(rk¯, mk¯) supE44 thi-1 λ¯recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 Φ80d lacZ ΔM15 deoR 特色: Suitable for cloning with large plasmid and cDNA library construction Allows blue-white colony screening. 應用: Cloning and subcloning Scale-up application Blue/white screening
Celemics, Inc. | All-in-one Library Preparation Kit
셀레믹스는 아래의 가이드라인에 따라 샘플을 준비할 것을 권장합니다. 아래 제시된 최소 샘플 요구 조건을 충족할 수 없는 샘플의 경우 별도로 문의해 주시기 바랍니다 ...
Celemics, Inc. | Library Preparation Kit
셀레믹스는 아래의 가이드라인에 따라 샘플을 준비할 것을 권장합니다. 아래 제시된 최소 샘플 요구 조건을 충족할 수 없는 샘플의 경우 별도로 문의해 주시기 바랍니다 ...
A Complete Solution for RNA-Seq Library Construction from Picogram Inputs
Generate stranded, Illumina®-ready cDNA libraries from 250 pg-10 ng of total mammalian RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian.
Module BSX-2019: Molecular Biology , Bangor University
The lectures build on the 1st year Principles of Life 1 module. Subjects covered include the organisation of the eucaryotic genome, DNA fingerprinting, human genetic defects, genome editing, cDNA library construction, the use of reporter genes etc.. The molecular biology practicals are designed to give experience in applied molecular methodologies covering a range of key technologies including the isolation of human DNA from cheek cells, restriction enzyme digestion of DNA, agarose gel electrophoresis, the polymerase chain reaction, the analysis of genetically modified plants etc. ...
Modiwl BSX-2019: Molecular Biology , Prifysgol Bangor
The lectures build on the 1st year Principles of Life 1 module. Subjects covered include the organisation of the eucaryotic genome, DNA fingerprinting, human genetic defects, genome editing, cDNA library construction, the use of reporter genes etc.. The molecular biology practicals are designed to give experience in applied molecular methodologies covering a range of key technologies including the isolation of human DNA from cheek cells, restriction enzyme digestion of DNA, agarose gel electrophoresis, the polymerase chain reaction, the analysis of genetically modified plants etc. ...
Xenbase Library: Summary for NICHD XGC Emb2 [species: Xenopus laevis]
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. NOTE: based on EST sequencing of several thousand clones from this library, there appears to be a low level of mouse background in this library; please use care when evaluating sequences from this library. Note: this is a Xenopus Gene Collection library. ...
Frontier Library: 2010
Today begins National Library Week, an annual celebration that highlights the value of all types of libraries and librarians. This years theme is Communities Thrive @your library. Libraries have long been integral to communities. They provide access to information for the entire community and also serve as meeting places and community centers. As we strive to build a community of inquiry here at Frontier, the library plays a central role in that process as well. Once you become a part of the Frontier community, you will always be a part of it, no matter where you may reside. But you are also a part of your home community. I thought we could take this opportunity to discuss libraries in your own community. Are you aware of the libraries in your community? If not, you might want to start by checking out www.worldcat.org. On their Find a Library page, you can enter a zip code or city name and see a list of libraries and the distance to them. Note that ALL types of libraries are shown, even ...
Roche KAPA RNA HyperPrep | GenomeWeb
Roche has launched KAPA RNA HyperPrep, a robust RNA library preparation solution for a range of sample types and input amounts. These kits provide a single-tube workflow and decrease processing time by 30 percent while maintaining quality, Roche said. The kits utilize a novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality RNA-seq libraries, Roche added. These strand-specific workflows can be completed in a standard workday while generating high-quality libraries from challenging samples such as formalin-fixed, paraffin-embedded tissue.
Rx Biosciences » Subtracted cDNA Library
Rx Biosciences prepares subtracted cDNA libraries after selectively removing the messages that are present in both, the control as well as the experimental samples. The subtracted library prepared by us is enriched in differentially expressed genes. Using in-house subtraction procedure or other PCR based techniques the libraries are constructed involving rigorous Q.C. at all the stages of the library construction. The libraries prepared by us are highly suitable for high throughput sequencing projects.. ...
In Silico Biology - Volume 7, issue 1 - Journals - IOS Press
Authors: de Souza Peres, Tarcísio , Costa, Fernando Ferreira , Alberto, Fernando Lopes Article Type: Research Article Abstract: We developed a Perl-based tool called LyM to determine the best factor for changes in the expression level for each transcript across two sets of expression libraries. LyM includes a Bayesian framework that analyzes the prior and posterior probability density function for each transcript considering the size of the libraries. To find out the best factor for change in each distribution, LyM was implemented with a binary search. In this work we aimed to validate …the performance of LyM tool using SAGE libraries from different human tissues. The results were compared with those generated by DGED (Digital Gene Expression Displayer), which worked as the gold standard, on the same data set, to assess accuracy. SAGE libraries were selected from CGAP for the following tissues (normal versus tumor): breast, colon, lung and stomach, consisting of eight SAGE libraries and ...
FTBO Anti Vibration Plate with Integrated Power Model,ESC,PDB,5V 12V BEC pdb board for APM2.5/2.6/2.8&PIXHAWK Flight...
restraints for crystallographic refinement. Input is in PDB format. Output is Pymol pdb: Using the Python debugger in Django - Minuti by Mike Tigas. 14 Sep 2010 Tutorial on using pdb, the Python debugger, within the Django runserver script.Illumina Sequencing Library Preparation for Highly Multiplexed . doi:10.1101/pdb.prot5448 Cold Spring Harb Protoc 2010. 2010: pdb.prot5448 ...
Fluidigm | Search
Juno™ is preprogrammed to perform multiple methods with push-button ease. Juno methods include targeted DNA sequencing library preparation, IFC control and thermal cycling for SNP genotyping upstream of Biomark™ HD or EP1™ systems, and IFC control for gene expression, copy number analysis or digital PCR upstream of Biomark HD. …. ...
Fluidigm | Search
Juno™ is preprogrammed to perform multiple methods with push-button ease. Juno methods include targeted DNA sequencing library preparation, IFC control and thermal cycling for SNP genotyping upstream of Biomark™ HD or EP1™ systems, and IFC control for gene expression, copy number analysis or digital PCR upstream of Biomark HD. …. ...
Rx Biosciences » Normalized cDNA Library
Rx Biosciences constructs normalized cDNA libraries in which high-copy gene sequences are selectively removed resulting into the enrichment of low copy and rare genes in the library. As per customers request we employ either the technology in which the double stranded cDNA clones of a regular library are converted into single stranded circles, hybridized with cRNA and unhybridized singled stranded circular plasmids are converted into double stranded plasmids or the normalization of high copy genes is performed at the mRNA level before library construction. PCR based technique is also used as per customers request and sample requirement.. ...
Addgene: DECIPHER Mouse shRNA Libraries - Pathway and Disease Targets
DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format deposited by Chenchik and Frangou.
PPT - Section I Gene library and screening PowerPoint presentation | free to download - id: 3c2165-YWNjZ
Molecular Biology Section I Gene library and screening I1 Genomic libraries I2 cDNA libraries I3 Screening procedures I1 Genomic libraries Representative gene ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3c2165-YWNjZ
Application Of Genomic Dna Library
Construction of a genomic DNA library with a TA vector - Genomic Library: cDNA Library: Definition: A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of
Gene library construction</span>...
GECCO is offering development and construction of directed evolution libraries or small focused libraries, where for example several residues are being randomized ...
cDNA library: NICHD XGC OO1
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library ...
Library Prep and NGS Pricing Descriptions - Genomic Sequencing and Analysis Facility User Support Wiki - UT Austin Wikis
Metagenomics assay: This service starts with normalized (i.e. equal concentration and volume) gDNA and includes amplification using one of the GSAF provided primer sets chosen by the customer as shown on this web page. The service is calibrated to deliver at least 10,000 2x250 bp paired-end sequences from the Illumina MiSeq platform for at least 90% of the samples submitted, assuming there is reasonable amount of DNA to amplify. To minimize jackpot effects, PCR is performed in triplicate for each sample and re-pooled. The library preparation is a two-step process, a gene-specific PCR then a second PCR to add the dual indexes. The PCR targets the bacterial 16S V4/V5 region, the 16S V4 region, or fungal ITS region, as specified by the client. Gel-based QC is performed on a sampling of 10% of the samples post amplification. Genomic DNA is expected as input; the GSAF does not provide DNA extraction services at this time. See this web page for sample input guidelines and note that DNA concentrations ...
matplotlib / Mailing Lists
Hi everyone, I am trying to build the latest svn trunk version of MPL on OS X 10.5. I am getting the following error: ld: in /Developer/SDKs/MacOSX10.4u.sdk/usr/local/lib/libPng.dylib, file is not of required architecture for architecture ppc After googling quite a bit, this seems to be quite a common problem. However, I could still not find an unambiguous solution. I have build libpng from source on my machine. The file output is: [email protected]:/usr/local/lib$ file libpng* libpng.3.dylib: Mach-O dynamically linked shared library i386 libpng.a: current ar archive random library libpng.dylib: Mach-O dynamically linked shared library i386 libpng.la: ASCII English text libpng12.0.dylib: Mach-O dynamically linked shared library i386 libpng12.a: current ar archive random library libpng12.dylib: Mach-O dynamically linked shared library i386 libpng12.la: ASCII English text It appears that the compiler expects that libpng will also have libraries for ppc. What do I do? Do I need to rebuild libpng to be ...
Whole Genome and Hybrid Capture - QIAGEN
QIAGENs library prep solutions enable the generation of high-quality, high conversion rate whole genome libraries suitable for direct sequencing or hybrid capture in as little as 2.5 hours, or from as little as 10pg of bacterial or 100pg of eukaryotic DNA. Discover the ways QIAseq library preparation kits maximize per-sample data quality while streamlining your laboratory processes today! ...
Yeast Two-Hybrid cDNA Library - Mate and Plate Libraries
Yeast two hybrid library is a highly complex, tissue-specific library. Screening yeast 2 hybrid library is now simple with Mate and Plate libraries.
Comparison of whole-genome bisulfite sequencing library preparation strategies identifies sources of biases affecting DNA...
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such as PCR amplification, are known to introduce biases, a systematic evaluation of biases in WGBS strategies is missing. We perform a comparative analysis of several commonly used pre- and post-bisulfite WGBS library preparation protocols for their performance and quality of sequencing outputs. Our results show that bisulfite conversion per se is the main trigger of pronounced sequencing biases, and PCR amplification builds on these underlying artefacts. The majority of standard library preparation methods yield a significantly biased sequence output and overestimate global methylation. Importantly, both absolute and relative methylation levels at specific genomic regions vary substantially
cDNA library construction and isolation of genes for candidate vaccine antigens from Chrysomya bezziana (the Old World...
cDNA library construction and isolation of genes for candidate vaccine antigens from Chrysomya bezziana (the Old World Screwworm fly)
Consider Bias in NGS Library Preparation | Genohub Blog
Library preparation for next-generation sequencing involves a coordinated series of enzymatic reactions to produce a random, representative collection of adapter modified DNA fragments within a specific range of fragment sizes. The success of next-generation sequencing is contingent on this proper processing of DNA or RNA sample. However as you go from isolating nucleic acid to…
Frontiers | Screening of an E. coli O157:H7 Bacterial Artificial Chromosome Library by Comparative Genomic Hybridization to...
Enterohemorrhagic E. coli (EHEC) O157:H7 can cause serious gastrointestinal and systemic disease in humans following direct or indirect exposure to the bacterium excreted in ruminant feces. The main colonization site of EHEC O157:H7 in cattle is the terminal rectum where the bacteria intimately attach to the epithelium and multiply in the intestinal mucus. This study aimed to identify genomic regions of EHEC O157:H7 that contribute to colonization and multiplication at this site. A bacterial artificial chromosome (BAC) library was generated from a derivative of the sequenced E. coli O157:H7 Sakai strain. The library contains 1152 clones averaging 150 kbp. To verify the library, clones containing a complete locus of enterocyte effacement (LEE) were identified by DNA hybridization. In line with a previous report, these did not confer a type III secretion capacity to the K-12 host strain. However, conjugation of one of the BAC clones into a strain containing a partial LEE deletion restored type III
Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
Custom-defined oligonucleotide collections have a broad range of applications in fields of synthetic biology, targeted sequencing, and cytogenetics. Also, they are used to encode information for technologies like RNA interference, protein engineering and DNA-encoded libraries. High-throughput parallel DNA synthesis technologies developed for the manufacture of DNA microarrays can produce libraries of large numbers of different oligonucleotides, but in very limited amounts. Here, we compare three approaches to prepare large quantities of single-stranded oligonucleotide libraries derived from microarray synthesized collections. The first approach, alkaline melting of double-stranded PCR amplified libraries with a biotinylated strand captured on streptavidin coated magnetic beads results in little or no non-biotinylated ssDNA. The second method wherein the phosphorylated strand of PCR amplified libraries is nucleolyticaly hydrolyzed is recommended when small amounts of libraries are needed. The third
HyperCap products provide an integrated next-generation sequencing (NGS) sample preparation solution that combines all reagents and accessories required for library preparation and target enrichment in a single workflow. The HyperCap Workflow v2.0 integrates high-efficiency KAPA Library Preparation Kits and KAPA Dual-Indexed Adapters with performance-optimized SeqCap® EZ probes and Roche Universal Blocking Oligo (UBO) Kit. The HyperCap Workflow v2.0 is an improved and streamlined version of our SeqCap EZ HyperCap Workflow, with the convenience of ordering and support from a single vendor, and is automation friendly.. KAPA HyperPrep and KAPA HyperPlus Library Preparation Kits are novel one-tube chemistry with optimally formulated enzymes selected through our directed evolution technology. They enable higher yields of adapter-ligated library and lower amplification bias resulting in higher library diversity, lower duplication rates and more uniform coverage compared to other products available in ...
Rheonix to Present Data on User-Defined Tests and Next-Generation Sequencing Library Preparation with its Automated Molecular...
Rheonix will present data from a user-defined assay for STIs and data that demonstrates new capabilities of its Encompass Optimum™ workstation in NGS
Bias in RNA-seq Library Preparation | RNA-Seq Blog
Although RNA sequencing (RNA-seq) has become the most advanced technology for transcriptome analysis, it also confronts various challenges. As we all know, the workflow of RNA-seq is extremely complicated and it is easy to produce bias. This may damage the quality of RNA-seq dataset and lead to an incorrect interpretation for sequencing result. Thus, our ...
Viruses strongly influence microbial population dynamics and ecosystem functions. However, our ability to quantitatively evaluate those viral impacts is limited to the few cultivated viruses and double-stranded DNA (dsDNA) viral genomes captured in quantitative viral metagenomes (viromes). This leaves the ecology of non-dsDNA viruses nearly unknown, including single-stranded DNA (ssDNA) viruses that have been frequently observed in viromes, but not quantified due to amplification biases in sequencing library preparations (Multiple Displacement Amplification, Linker Amplification or Tagmentation).Here we designed mock viral communities including both ssDNA and dsDNA viruses to evaluate the capability of a sequencing library preparation approach including an Adaptase step prior to Linker Amplification for quantitative amplification of both dsDNA and ssDNA templates. We then surveyed aquatic samples to provide first estimates of the abundance of ssDNA viruses.Mock community experiments confirmed ...
A novel process of viral vector barcoding and library preparation enables high-diversity library generation and recombination...
Detailed characterization and mapping of oligonucleotide function in vivo is generally a very time consuming effort that only allows for hypothesis driven subsampling of the full sequence to be analysed. Recent advances in deep sequencing together with highly efficient parallel oligonucleotide synthesis and cloning techniques have, however, opened up for entirely new ways to map genetic function in vivo. Here we present a novel, optimized protocol for the generation of universally applicable, barcode labelled, plasmid libraries. The libraries are designed to enable the production of viral vector preparations assessing coding or non-coding RNA function in vivo. When generating high diversity libraries, it is a challenge to achieve efficient cloning, unambiguous barcoding and detailed characterization using low-cost sequencing technologies. With the presented protocol, diversity of above 3 million uniquely barcoded adeno-associated viral (AAV) plasmids can be achieved in a single reaction through ...
PGN004 PureGenome™ Next Generation Sequencing Library Validator Kit from MilliporeSigma | SelectScience
Read independent reviews on PGN004 PureGenome™ Next Generation Sequencing Library Validator Kit from MilliporeSigma on SelectScience
ChIPmentation: fast, robust, low-input ChIP-seq for histones and transcription factors | Nature Methods
ChIPmentation combines chromatin immunoprecipitation with on-bead tagmentation for rapid and highly robust ChIP-seq library preparation. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase (tagmentation). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.
Fast and Efficient Preparation of Next Generation Sequencing Libraries | SelectScience
EMD Millipore today launched PureGenome™ kits and reagents for rapid and efficient Next Generation Sequencing (NGS) sample preparation. The kits enable NGS library preparation in less than two hours.
Sequencing from a normalized cDNA library of onion revealed 59.9% unique sequences, one of the highest values reported for plant EST collections (Table 1). Large-scale sequencing at TIGR from tissue-specific nonnormalized tomato cDNA libraries revealed ,40% unique ESTs when sequencing ,20,000 random clones. Sampling of EST data sets at TIGR has shown that sequencing fewer clones from different nonnormalized libraries reduced the redundancy to 50% and sequencing from normalized animal cDNA libraries reduced the redundancy to ,30% (Smith et al., 2001). Given continually lower sequencing costs, it may be more cost-effective to sequence fewer random clones from numerous nonnormalized libraries to maximize the return on each sequencing reaction. However, the cost of synthesizing numerous cDNA libraries must be considered, and this approach may not reveal large numbers of relatively low-copy transcripts.. Our onion ESTs represent the first large collection of non-Poales expressed sequences for the ...
Assay Scheme & Configuration of Chromium Single Cell DNA Libraries -Technical Note -Official 10x Genomics Support
CG000162_TechNote_AssayScheme&Configuration_Chromium_scDNA_Libraries_RevA.pdf. This Technical Note presents a detailed description of the assay configuration for Chromium Single Cell DNA libraries. Individual steps during library construction, including primer sequences, adaptor sequences, and the final library construct, are outlined here, and may serve as a reference to customize the library preparation workflow.. FOR USE WITH. ...
Illumina | tebu-bios blog
Next generation sequencing has quickly become the preferred method over tiling arrays for most genomics and transcriptomics needs. The major exception has been the study of microRNAs, where highly sensitive probe arrays such as the 3D-Gene® miRNA profiling platform are still widely used. A large part of the reason for the persistence of array dominance in small RNA expression profiling is caused by the variability introduced in sequencing library prep protocols involving complicated hands-on PAGE purification steps.. The CleanTag™ Ligation Kit for Small RNA Library Preparation now allows users to remove the Gel Purification steps from their protocols and shift to more automated bead purification protocols. This is particularly important for cases when RNA quantity is limiting. Traditional small RNA library prep protocols will result in the formation of adapter dimers (similar to primer dimers) when RNA quantities are limiting, thus greatly reducing the number of usable reads.. ...
ezRAD: a simplified method for genomic genotyping in non-model organisms [PeerJ]
Here, we introduce ezRAD, a novel strategy for restriction site-associated DNA (RAD) that requires little technical expertise or investment in laboratory equipment, and demonstrate its utility for ten non-model organisms across a wide taxonomic range. ezRAD differs from other RAD methods primarily through its use of standard Illumina TruSeq library preparation kits, which makes it possible for any laboratory to send out to a commercial genomic core facility for library preparation and next-generation sequencing with virtually no additional investment beyond the cost of the service itself. This simplification opens RADseq to any lab with the ability to extract DNA and perform a restriction digest. ezRAD also differs from others in its flexibility to use any restriction enzyme (or combination of enzymes) that cuts frequently enough to generate fragments of the desired size range, without requiring the purchase of separate adapters for each enzyme or a sonication step, which can further decrease the cost
An Improved Genotyping by Sequencing (GBS) Approach Offering Increased Versatility and Efficiency of SNP Discovery and...
Highly parallel SNP genotyping platforms have been developed for some important crop species, but these platforms typically carry a high cost per sample for first-time or small-scale users. In contrast, recently developed genotyping by sequencing (GBS) approaches offer a highly cost effective alternative for simultaneous SNP discovery and genotyping. In the present investigation, we have explored the use of GBS in soybean. In addition to developing a novel analysis pipeline to call SNPs and indels from the resulting sequence reads, we have devised a modified library preparation protocol to alter the degree of complexity reduction. We used a set of eight diverse soybean genotypes to conduct a pilot scale test of the protocol and pipeline. Using ApeKI for GBS library preparation and sequencing on an Illumina GAIIx machine, we obtained 5.5 M reads and these were processed using our pipeline. A total of 10,120 high quality SNPs were obtained and the distribution of these SNPs mirrored closely the ...
Technical advance in targeted NGS analysis enables identification of lung cancer risk-associated low frequency TP53, PIK3CA,...
Standardized Nucleic Acid Quantification for SEQuencing (SNAQ-SEQ) is a novel method that utilizes synthetic DNA internal standards spiked into each sample prior to next generation sequencing (NGS) library preparation. This method was applied to analysis of normal appearing airway epithelial cells (AEC) obtained by bronchoscopy in an effort to define a somatic mutation field effect associated with lung cancer risk. There is a need for biomarkers that reliably detect those at highest lung cancer risk, thereby enabling more effective screening by annual low dose CT. The purpose of this study was to test the hypothesis that lung cancer risk is characterized by increased prevalence of low variant allele frequency (VAF) somatic mutations in lung cancer driver genes in AEC. Synthetic DNA internal standards (IS) were prepared for 11 lung cancer driver genes and mixed with each AEC genomic (g) DNA specimen prior to competitive multiplex PCR amplicon NGS library preparation. A custom Perl script was developed to
CATS - a novel library preparation method for ultrasensitive deep sequencing of extracellular circulating RNA and DNA
Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of nucleic acids still rem
CDNA library - Wikipedia
A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism and are stored as a library. cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue-specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library. cDNA is created from a mature mRNA from a eukaryotic cell with the use of reverse transcriptase. In eukaryotes, a poly-(A) tail (consisting of a long sequence of adenine nucleotides) distinguishes mRNA from tRNA and rRNA ...
Dr. Matt Rise | Ocean Sciences | Memorial University of Newfoundland
I develop and use genomic resources, including microarrays, in studies related to fish health. DNA microarrays allow a researcher to analyze relative expression levels of thousands of genes simultaneously. In my laboratory, experiments involving genomic techniques are used to identify the key genes involved in biological processes such as reproduction, development, growth, and immune responses to pathogens. We also study the transcriptomic and behavioural responses of fish exposed to environmental stressors including toxicants (e.g. pesticides, heavy metals). Some of the genomic techniques that we utilize include DNA microarray hybridizations, quantitative reverse transcription - polymerase chain reaction (QPCR), and high-complexity cDNA library construction and characterization. ...
Quantitative comparison of EST libraries requires compensation for sys by D Liu and J H. Graber
BACKGROUND: Publicly accessible EST libraries contain valuable information that can be utilized for studies of tissue-specific gene expression and processing of individual genes. This information is, however, confounded by multiple systematic effects arising from the procedures used to generate these libraries. RESULTS: We used alignment of ESTs against a reference set of transcripts to estimate the size distributions of the cDNA inserts and sampled mRNA transcripts in individual EST libraries and show how these measurements can be used to inform quantitative comparisons of libraries. While significant attention has been paid to the effects of normalization and substraction, we also find significant biases in transcript sampling introduced by the combined procedures of reverse transcription and selection of cDNA clones for sequencing. Using examples drawn from studies of mRNA 3-processing (cleavage and polyadenylation), we demonstrate effects of the transcript sampling bias, and provide a
Training at your library: we asked, you answered | NoveList Blog | EBSCOhost
Most of the respondents said their library did not have a dedicated trainer or staff development coordinator at their library. The 18% of libraries with one or more full-time trainers were from libraries that serve larger population. For example, 38% of libraries serving 100 thousand to 500 thousand people and 32% of libraries serving over 500 thousand people have full-time trainers.. A couple interesting notes to come out of the question about in-house training: at least one library focuses training on new staff with current staff only receiving training when policies and procedures change. Another library pointed out that they have staff members train on their expertise, from example youth services coordinators train on youth topics and human resources coordinates safety training, onboarding, and other system-wide concerns.. Libraries do bring in outside trainers, though again this varies by library size. Larger libraries are more likely to already bring in outside trainers or to plan to in ...
Re)Claiming Space: Two Subject Libraries at the Heart of the Academic Enterprise - Association of Collegiate Schools of...
Lucy Campbell and Barbara Opar, column editors. Column by David Eifler, Librarian, Environmental Design Library, UC Berkeley and Maya Gervits, Director, Littman Architecture Library, NJIT. (Re)Claiming Space: Two Subject Libraries at the Heart of the Academic Enterprise. The development of digital technology has led to predictions that the library as a physical space will cease to exist. However, the physical world of libraries has not been replaced by a digital one, rather they are developing in parallel. Architect Geoffrey Freeman acknowledges that Whereas the Internet has tended to isolate people, the library, as a physical place, has done just the opposite. Library scholar Michael Gorman notes that among other things, libraries are valued for being a focal point of a community, the heart of the university, the collective memory of a research institution, a place in which all are welcome and a source of power through knowledge.. University art and architecture libraries are no different and ...
New insights into genomic selection through population-based non-parametric prediction methods
References. Ammiraju, J.S.S.; Luo, M.; Goicoechea, J.L.; Wang, W.; Kudrna, D.; Mueller, C.; Talag, J.; Kim, H.; Sisneros N.B.; Blackmon, B.; Fang, E.; Tomkins, J.B.; Brar, D.; Mackill, D.; MacCouch, S.; Kurata, N.; Lambert, G.; Galbraith, D.W.; Arumuganathan, K.; Rao, K.; Walling, J.G.; Gill, N.; Yu, Y.; Sanmiguel, P.; Soderlund, C.; Jackson, S.; Wing, R.A. 2006. The Oryza bacterial artificial chromosome library resource: construction and analysis of 12 deep-coverage large-insert BAC libraries that represent the 10 genome types of the genus Oryza. Genome Research 16: 140-147. [ Links ] Azevedo, C.F.; Resende, M.D.V.; Silva, F.F.; Viana, J.M.S.; Valente, M.S.F.; Resende Junior, M.F.R.; Muñoz, P. 2015. Ridge, Lasso and Bayesian additive-dominance genomic models. BMC Genetics 16: 105. [ Links ] Bennewitz, J.; Meuwissen, T.H.E. 2010. The distribution of QTL additive and dominance effects in porcine F2 crosses. Journal of Animal Breeding and Genetics 127: 171-179. [ Links ] De los Campos, G.; ...
Xenbase Library: Summary for NIH XGC tropOvi1 [species: Xenopus tropicalis]
Description: The library was prepared from 5 ug of poly A+ RNA by oligo-dT priming (5- ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTTTTTV-3) and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5-AATTCGGCACGAGG-3) followed by kinasing adapters and by XhoI digestion, the cDNA was size selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1000 bp were ligated into EcoRI/XhoI-digested pCS107. Average insert size 1745 bp. Reference for library construction: Current Genomics 4, 635-644. Library constructed by Michelle Tabb and Bruce Blumberg (Dept of Developmental and Cell Biology, University of California, Irvine). ...
Difference between revisions of Library Generation - OpenWetWare
Genetic libraries are collections of genes present in some recombinant DNA form so they can be propagated. When people refer to screening a library they usually have some phenotype that they are able to select or screen for and evaluate a large number of library clones to look for a gene that alters the phenotype. People interested in eukaryotic biology usually make cDNA libraries that are derived from pools of mRNA isolated from an organism of interest. This allows them to isolate DNA fragments that encode proteins or RNA that are produced form the spliced form of the RNAs found in the cells. It has been a long time since I have worked with cDNA libraries, so I wont go into that here (perhaps someone in another group can add a section?). For example, suppose we have a strain of bacteria that cant grow on lactose (like Salmonella) and we are interested in finding genes that are needed for lactose metabolism. First, we prepare a plasmid that has been digested with two different restriction ...
rio: A standard library for Haskell
library and reexport it from this library.. This library attempts to define a set of libraries as standard, meaning they are recommended for use, and should be encouraged as dependencies for other libraries. It does this by depending on these libraries itself, and reexporting their types and functions for easy use.. Beyond the ecosystem effects we hope to achieve, this will hopefully make the user story much easier. For a new user or team trying to get started, there is an easy library to depend upon for a large percentage of common functionality.. See the dependencies of this package to see the list of packages considered standard. The primary interfaces of each of these packages is exposed from this library via a ...
Stranded RNA-seq - QIAGEN
For applications such as gene expression, fusion gene or mutation detection, QIAseq Stranded mRNA Select Kits include an optimized mRNA enrichment protocol with all the reagents and components required to build high-quality RNA-seq libraries. The QIAGEN proprietary CleanStart PCR Mix included with kits efficiently and uniformly amplifies the RNA-seq library regardless of the GC content of the template, while also protecting against PCR contamination. Kits are compatible with fresh, as well as FFPE samples. The streamlined, 4-5 hour protocol allows the generation of NGS libraries, library QC measurements and the start of an NGS run in just one working day ...
Harry Levine (sociologist)
"Levine, Harry Gene". Library of Congress. Retrieved 2018-02-14. "Harry Levine". QC Sociology. Retrieved 2018-02-14. Dwyer, Jim ... Harry Gene Levine (born April 1, 1945) is an American sociologist known for his research on alcohol and illicit drugs in ...
Key West Citizen
Library of Congress. Roberts, Gene; Kunkel, Thomas; Layton, Charles, eds. (2001). Leaving Readers Behind: The Age of Corporate ... Key West Citizen (Key West, Fla.) Florida Digital Newspaper Library. George A. Smathers Libraries at the University of Florida ...
Detroit: Detroit Library Commission. Scott, Gene (2001). Detroit Beginnings: Early Villages and Old Neighborhoods. Detroit: ... Chicago: American Library Association. ISBN 0838906435. Ewen, Lynda Ann (1978). Corporate Power and Urban Crisis in Detroit. ... Bentley Historical Library. Retrieved on January 8, 2014. McGrail, Lucas. The Carlton. Historic Detroit. Retrieved April 1, ...
Open Library. Amdahl, Gene Myron (1951). The Logical Design of an Intermediate Speed Digital Computer (PDF). University of ... Tomash, Erwin (2008). "The Erwin Tomash Library on the History of Computing: An Annotated and Illustrated Catalog". www.cbi.umn ...
Transmembrane protein 251
"TMEM251 transmembrane protein 251 [Homo sapiens (human)]". NCBI Gene. U.S. National Library of Medicine. Retrieved 9 May 2015 ... which is a set of similar genes that are formed by duplication of an original gene, is around 120 genes. Gene duplication, ... The TMEM251 gene is located on human chromosome 14, at 14q32.12, on the plus strand. The gene size is 1,277 base pairs. It ... The TMEM251 gene first appeared on the planet around 400 million years ago (MYA), since the most distant orthologs are found in ...
Rap GTP-binding protein
Entrez Gene. United States National Library of Medicine. Rousseau-Merck MF, Pizon V, Tavitian A, Berger R (1990). "Chromosome ... Human genes that encode Ras-related proteins include: RAP1A, RAP1B RAP2A, RAP2B, RAP1C RAB5C "RAP1A RAP1A, member of RAS ... rap+GTP-Binding+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ... from the United States National Library of Medicine, which is in the public domain. Biology portal v t e. ...
"Entrez Gene: IYD Iodotyrosine deiodinase". Entrez Gene. United States National Library of Medicine. Rosenberg IN, Goswami A ( ... Moreno JC (2003). "Identification of novel genes involved in congenital hypothyroidism using serial analysis of gene expression ... National Library of Medicine. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library ... The gene encoding this enzyme has been recently identified. The sequence of amino acids of iodotyrosine deiodinase is highly ...
"Founder of the Town of Heppner". University of Oregon Libraries; Eugene, OR. Morning Oregonian., February 18, 1905, Page 11, ... "Town Topics". The Oregon Daily Journal., February 18, 1905, Image 5; Image provided by: University of Oregon Libraries; Eugene ... University of Oregon Libraries; Eugene, OR. "Henry Heppner Died Thursday". East Oregonian : E.O., February 17, 1905, Daily ... Eugene, OR". December 9, 1903. Library, University of Oregon, Knight. "East Oregonian : E.O. (Pendleton, OR) 1888-current, ...
"Gene: BTBD9 - ENSG00000183826". bgee.org. Retrieved 2020-04-14. "BTBD9 gene". U.S. National Library of Medicine. November 26, ... The gene is located on the short arm of chromosome 6. This domain is understood to contain genes encoding protein-protein ... BTBD9 is a human gene. BTBD9 encodes a protein that is in a subgroup of BTB(POZ) proteins, which contribute to the forming of ... January 2007). "Genome-wide atlas of gene expression in the adult mouse brain". Nature. 445 (7124): 168-76. Bibcode:2007Natur. ...
"OPN1LW gene". U.S National Library of Medicine. Genetics Home Reference. Retrieved November 29, 2017. Imamoto Y, Shichida Y ( ... Protanomaly occurs when a partially functional hybrid OPN1LW gene replaces the normal gene. Opsins made from these hybrid genes ... They are in a tandem array, composed of a single OPN1LW gene which is followed by one or more OPN1MW genes. The locus control ... Recombination events usually begin with misalignment of an OPN1LW gene with an OPN1MW gene and are followed by a certain type ...
Eastern New Mexico University
Bundy, Gene. "Jack Williamson Science Fiction Library". ENMU. Retrieved August 31, 2012. "The Dr. Antonio Gennaro Natural ... Golden Library The Golden Library features General Collections, Special Collections and Government Information. Special ... Jack Williamson Science Fiction Library The Jack Williamson Science Fiction Library is part of the Special Collections and has ... The building now houses the Golden Library, the Runnels Gallery and the offices of Tutoring and Supplemental Instruction and ...
"Inventory". Gene Weltfish Pawnee Field Notes. Newberry Library. 1935. (See also: Gene Weltfish.) "Pawnee Nation College".. ... Weltfish, Gene (1977). The Lost Universe: Pawnee life and culture. Lincoln, NE: University of Nebraska Press. ISBN 0-8032-5871- ... Writing in the 1960s, the historian Gene Weltfish drew from earlier work of Wissler and Spinden to suggest that the sacrificial ... Newberry Library. Indiana University Press. ISBN 978-02533-1502-1. Lagace, Robert O. "Pawnee: Culture summary". Ethnographic ...
One Thousand Ways to Make $1000
"Lines From The Library". The Eugene Guard. Eugene, Oregon. August 18, 1940. p. 11. Retrieved July 7, 2017 - via Newspapers.com ... A voracious reader his entire life, at age seven he read a book he borrowed from the library, One Thousand Ways to Make $1000, ...
Jehovah's Witnesses publications
Libraries Unlimited. Eugene V. Gallagher; W. Michael Ashcraft (2006). Introduction to New and Alternative Religions in America ... Many of these publications are also available from their website in the Watchtower Online Library. In addition to Jehovah's ... Others, such as the organizational manual Organized to Do Jehovah's Will and Watchtower Library (containing the Watch Tower ... but may be supplied to other interested individuals on request or made available in public libraries. These include the ...
Open Library Rapture Ready Long, Gene. Beverly Carradine Prince of Holiness Writers (Allegheny Publications, 2003) ISBN 978-0- ... Beverly Carradine, Gene A. Long, Holiness Data Ministry (2475) http://wesley.nnu.edu/wesleyctr/books/2401-2500/HDM2475.PDF ...
Daniel Sheldon Norton
"Norton, Daniel Sheldon - Legislator Record". Minnesota Legislative Reference Library. Smalley, Eugene Virgil (1896). A history ...
Biology and consumer behaviour
"What is a gene?". U.S National Library of Medecine. 2012. Retrieved 12 April 2012. ForaTV 2010. Can your behaviour affect your ... However genes have a protein wrapping the gene that can influence the gene expression and therefore phenotypes to the ... Richard Dawkins outlines in The Selfish Gene (1976) that humans are machines made of genes, and genes are the grounding for ... "The genes are the agents of nurture and are more likely to be affecting appetite more than aptitude". Genes do not make the ...
List of last surviving veterans of military operations
"Eugene W. Lee Collection". Library of Congress. Kloth, Hans Michael (27 May 2008). "Letzter Veteran der Mittelmächte gestorben ... Kelly D. Selby to Speak on black Civil War soldiers at Cuyahoga Falls Library". UPublish. Akron Beacon Journal. Retrieved 30 ... Los Angeles Examiner (1912). Press Reference Library: Notables of the Southwest. Los Angeles Examiner. p. 290. Retrieved 9 June ... Retrieved 3 December 2020 - via Wiley Online Library. CS1 maint: discouraged parameter (link) Grant, David. "Frederick Henry ...
Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD (2006). "Amplification of complex gene libraries by ... DNA sequencing may be used to determine the sequence of individual genes, larger genetic regions (i.e. clusters of genes or ... Making DNBs replaces the need to generate PCR copies of the library on the flow cell and as such can remove large proportions ... Min Jou W, Haegeman G, Ysebaert M, Fiers W (May 1972). "Nucleotide sequence of the gene coding for the bacteriophage MS2 coat ...
Battle of Pelusium (343 BC)
Modern Library. Ray Fred Eugene, Jr. (2012). Greek and Macedonian Land Battles of the 4th Century B.C.: A History and Analysis ...
Schimmel, Caroline F. (November 1978). "The Man who Printed Books at the YMCA." Columbia Library Columns. Ettenberg, Eugene M ... PA Photography by John Fass at New York Public Library John Fass' Hammer Creek Press Books at New York Public Library John Fass ... Burke, Jackson; Ettenberg, Eugene M. (1998). John S. Fass and the Hammer Creek Press. Melbert B. Cary, Jr. Graphic Arts ... John Archer organized an exhibition of Hell-Box Press and Hammer Creek Press imprints in 1953 for the New York Public Library. ...
History of Alaska
U.S. Geological Survey (August 24, 2005). Retrieved on 2007-04-08 "Clarence L. Andrews Collection". UO Libraries. Eugene, ... Library of Congress The Papers of Clarence L. Andrews at Dartmouth College Library. ... Austin Eugene "Cap" Lathrop (1865-1950), industrialist, founder of some of Alaska's oldest radio stations and builder of ... University of Washington Libraries Digital Collections John E. Thwaites Photographs Images of Southeastern Alaska from 1905- ...
Peter II, Count of Savoy
London: British Library. 1916. Cox, Eugene L. (1974). The Eagles of Savoy. Princeton: Princeton University Press. ISBN ... Cox, Eugene L. (1967). The Green Count of Savoy. Princeton, New Jersey: Princeton University Press. LCCN 67-11030. Pollock, M. ... British Library 1916, p. 78. Chisholm, Hugh, ed. (1911). "Richmond, Earls and Dukes of" . Encyclopædia Britannica. 23 (11th ed ...
Michigan's 98th House of Representatives district
"Legislator Details - Eugene R. Cater". Library of Michigan. Retrieved August 17, 2020. "Legislator Details - Dennis O. ... "Legislator Details - Jim Stamas". Library of Michigan. Retrieved August 17, 2020. "Legislator Details - Gary Glenn". Library of ... "Legislator Details - James McNutt". Library of Michigan. Retrieved August 17, 2020. "Legislator Details - Tony Stamas". Library ... Cawthorne". Library of Michigan. Retrieved August 17, 2020. "Legislator Details - Jeff Dongvillo". Library of Michigan. ...
Sense (molecular biology) Polyak, Kornelia (2003). "Cancer Medicine 6 Overview: Gene Structure". US National Library of ... But keep in mind that sometimes, such as in prokaryotes, overlapping genes on opposite strands means the sense for one mRNA can ... Nakayama, Tomohiro (2007). "Overlapping Genes in the Human Genome". International Journal of Biomedical Science : Ijbs. 3 (1): ... It is ultimately the gene product, or mRNA, that dictates which strand of one segment of dsDNA we call sense or antisense. ...
Image provided by University of Oregon Libraries; Eugene, OR. Nelson, Mrs. Norman, Mrs. Omar Rietmann, Mrs. Anne Smouse, Mrs. ... Heppner gazette-times., March 07, 1963; University of Oregon Libraries 1501 Kincaid Street Eugene, OR 97403-1299; Image ... University of Oregon Libraries 1501 Kincaid Street Eugene, OR 97403-1299; Image provided by: Morrow County Museum; Heppner, OR ... University of Oregon Libraries 1501 Kincaid Street Eugene, OR 97403-1299; Image provided by: Morrow County Museum; Heppner, OR ...
University of Oregon Libraries; Image provided by: University of Oregon Libraries; Eugene, OR. 9 Oct 1910. Retrieved 11 ... University of Oregon Libraries, Image provided by: University of Oregon Libraries; Eugene, OR (March 23, 1911). "Heppner ... University of Oregon Libraries; Image provided by: Morrow County Museum; Heppner, OR. 3 January 1952. University of Oregon ... University of Oregon Libraries, Image provided by: Morrow County Museum; Heppner, OR (October 4, 1973). "Heppner Gazette-Times ...
D. B. Newton
University of Oregon Libraries. Eugene, Oregon. 2007. Retrieved September 2, 2017 - via Archives West. CS1 maint: discouraged ... Popular Library (1975) Trail of the Bear, Popular Library (1975) Broken Spur, Berkley (1977) As "Dwight Bennett" Stormy Range, ... 1981) As "Ford Logan" Fire in the Desert, Ballantine Books (1954) As "Dan Temple" Bullet Lease, Popular Library (1957) Gun and ... Nineteen linear feet of the author's papers are held at the University of Oregon Libraries, Special Collections and University ...
Bibliography of encyclopedias: biology
Sheehy, Eugene P., ed. (1986). Guide to Reference Books (Tenth ed.). Chicago and London: American Library Association. ISBN 978 ... Distributed in the U.S. to the Bookstore and library trade by National Book Network. p. 960. ISBN 978-0-7938-0656-0. DK ... Distributed in the U.S. to the Bookstore and library trade by National Book Network. p. 912. ISBN 978-0-7938-1284-4. Kelsey- ... Talbot, C. H., Eugene Ashby Hammond. The medical practitioners in medieval England: A biographical register. Wellcome ...
Bibliography of encyclopedias
Sheehy, Eugene P., ed. (1986). Guide to Reference Books (Tenth ed.). Chicago and London: American Library Association. ISBN 978 ... Who's who in library and information services. American Library Association, 1982. ISBN 0838903517. Pioneers of library and ... ISBN 978-0-313-33158-9. Music Library, Stanford University. Music library association obituary index. Music Library, Stanford ... American Library Association, 1991. Claghorn, Charles Eugene. Women composers and songwriters: A concise biographical ...
Socialist Sunday School
Eugene V. Debs, "Socialist Sunday School," Socialist News [Cleveland], vol. 1, no. 40 (Sept. 4, 1915), pg. 3. ... including an extensive proposed list of books for SSS libraries. ...
人類基因組 - 维基百科，自由的百科全
基因劑量（Gene dosage）會對人類的表現型產生龐大的影響，對於染色體中造成疾病的複寫、省略與分裂等現象的形成擁有一定的角色。例如唐氏症患者（21號染色體為三體）有較高的比率得到阿茲海默症，可能是因為與阿茲海默症有關的類澱粉前趨蛋白基因（位在21號 ... 英文）National Library of Medicine human genome viewer .. *（英文）UCSC Genome Browser. ... of which more
... s and Salts at the US National Library of Medicine Medical Subject Headings (MeSH) ... also known by its gene name NR1H4. Another bile acid receptor is the cell membrane receptor known as G protein- ... "Conjugated bile acid-activated S1P receptor 2 is a key regulator of sphingosine kinase 2 and hepatic gene expression" ...
Find this book in the Eugene, Oregon Public Library catalog. * Find this book in the Multnomah County, Oregon Library catalog ... Public libraries. * Find this book (and library resources in other formats) at your local library through the National Library ... Find this book at the Gothenburg Public Library library catalogue. * Find this book at the Malmö City Library library catalogue ... Public libraries. * Find this book in the Barrie Public Library catalogue. * Find this book in the Burnaby Public Library ...
Gene Weltfish. This pamphlet was intended for American troops and set forth, in simple language with cartoon illustrations, the ... Archives and Special Collections Library. *Benedict, Ruth. 1931. Tales of the Cochiti Indians. Bureau of American Ethnology ...
"Mean" Gene Kelton, singer-songwriter, blues musician, and band leader of Mean Gene Kelton & The Die Hards ... The current Sterling Library was dedicated in 1963. The library's space increased to 50,500 square feet (4,690 m2) after bond ... The original Goose Creek Library opened in 1925; the first county library in Texas, it was funded by the private donations of ... Located on the back corner of the library on W. Texas Avenue, this is a life-sized book shelf. The second ...
This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on ... This article on a gene on human chromosome 2 is a stub. You can help Wikipedia by expanding it.. *v ...
"Entrez Gene: NMT1 N-myristoyltransferase 1". Rajala RV, Datla RS, Moyana TN, et al. (2000). "N-myristoyltransferase". Mol. Cell ... 1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107-13. doi:10.1006/abio. ... is an enzyme that in humans is encoded by the NMT1 gene. GRCh38: Ensembl release 89: ENSG00000136448 - Ensembl, May 2017 GRCm38 ...
These gene products are important in determining cell fates during normal development and in maintaining homeostasis, or they ... Catenins at the US National Library of Medicine Medical Subject Headings (MeSH). ... Mutations in genes encoding these proteins can lead to inactivation of cadherin cell adhesions and elimination of contact ... Mutations in catenin genes can cause loss of contact inhibition that can promote cancer development and tumor formation. ...
Berkowitz, S.A., Philo, J.S. Monitoring the Homogeneity of Adenovirus Preparations (a Gene Therapy Delivery System) Using ... Article on "Centrifugation" retrieved on 15 October 2013 from http://www.lenntech.com/library/clarification/clarification/ ...
Lang, M.A. and Nebert, D.W. (1981). "Structural gene products of the Ah locus. Evidence for many unique P-450-mediated ... Wiley Classics Library. ISBN 0471303097. http://books.google.com/books/about/Enzyme_Kinetics.html?id=T60oAAAAYAAJ. ...
It has a core set of 1553 genes, plus 154 genes in its virulome, which contribute to virulence and 176 genes that maintain a ... PATH's Vaccine Resource Library pneumococcal resources. *. Centers for Disease Control and Prevention (2012). "Ch. 16: ... For instance, the Xisco gene was recently described as a biomarker for PCR-based detection of S. pneumoniae and differentiation ... In S. pneumoniae, at least 23 genes are required for transformation. For a bacterium to bind, take up, and recombine exogenous ...
They are labelled "Type I" if the defective gene is for an enzyme involved in the assembly or transfer of the Glc3Man9GlcNAc2- ... oligosaccharyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH) ...
... Research Archive Collection Guide - University of Iowa Libraries' Rita Benton Music Library. ... Eugene Rousseau, the classical saxophonist, is one of his former students. Much of his music was published by Rubank, Inc. ... University of Iowa Libraries. Spring 2004. Archived from the original (PDF) on 2008-06-25. Retrieved 2008-02-26.. ...
a b c d e Eugene Kiely, Medicaid Estate Recovery Program, FactCheck.org, Annenberg Public Policy Center, University of ... Center for Health Care Strategies, CHCS Extensive library of tools, briefs, and reports developed to help state agencies, ...
"Identification of significant association and gene-gene interaction of GABA receptor subunit genes in autism". Am. J. Hum. ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ McLean PJ, Farb DH, Russek SJ (Aug 1995 ... Gamma-aminobutyric acid receptor subunit alpha-4 is a protein that in humans is encoded by the GABRA4 gene. ...
America in the King Years
Memorial Library, Washington, D.C.. *Dr. Martin Luther King Jr. Library, San Jose ... The Race Beat by Gene Roberts and Hank Klibanoff (2007). *What Hath God Wrought by Daniel Walker Howe (2008) ...
These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ... U.S. National Library of Medicine. Retrieved 7 June 2020.. *^ Zhanel G, Critchley I, Lin LY, Alvandi N (January 2019). " ... Genes. Acne appears to be strongly inherited; genetics explain 81% of the variation in the population. Studies ... among others. The 308 G/A single nucleotide polymorphism variation in the gene for TNF is associated with an increased risk ...
"National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ a b "Entrez Gene: zona pellucida ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... "National Center for Biotechnology Information, U.S. National Library of Medicine.. *^ "Mouse PubMed Reference:". ... Gene ontology. Molecular function. • acrosin binding. • GO:0001948 protein binding. • carbohydrate binding. • identical protein ...
"Fibromyalgia Library. ProHealth.com. Retrieved 2008-11-01.. *Fight Club for Flies video, Science Take, New York Times, February ... organization of the gene, chromosome localization, and functional expression of cDNA clones". Biochemistry. 30 (44): 10640-6. ... are produced from a polyprotein precursor after differential splicing of the preprotachykinin A gene. The deduced amino acid ... "C/EBPbeta couples dopamine signalling to substance P precursor gene expression in striatal neurones". Journal of Neurochemistry ...
Apolipoprotein (apo)C4 gene is a member of the apolipoprotein C gene family. It is expressed in the liver and has a predicted ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ... Apo C4 is a 3.3-kb gene consisting of 3 exons and 2 introns; it is located 0.5 kb 5' to the APOC2 gene. ... This article on a gene on human chromosome 19 is a stub. You can help Wikipedia by expanding it. *v ...
Library of Congress ID: 61-11617.. *^ Vaisrub, Samuel; A. Denman, Michael; Naparstek, Yaakov; Gilon, Dan (2008). "Medicine". ... Many modern molecular tests such as flow cytometry, polymerase chain reaction (PCR), immunohistochemistry, cytogenetics, gene ... Genetics is the study of genes, and their role in biological inheritance. ... as the causative genes of most monogenic genetic disorders have now been identified, and the development of techniques in ...
"Eugene Register-Guard. 26 January 1976. Retrieved 27 June 2010.. *^ a b "Explore Our Past:2000 - present". British Airways. ... Retrieved 6 November 2019 - via National Library of Australia.. *^ "Worst midair crash ever claims 176 in Yugoslavia". ...
... end-enriched cDNA library". Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.. ... Paired box gene 8, also known as PAX8, is a protein which in humans is encoded by the PAX8 gene. ... This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode ... This article incorporates text from the United States National Library of Medicine, which is in the public domain. ...
"Modern Library. 1998. Retrieved March 4, 2015.. *^ a b Brian Urquhart (March 26, 2009). "What You Can Learn from Reinhold ... "Reinhold Niebuhr, Washington's Favorite Theologian" by Gene Zubovich, "Religion & Politics", April 25, 2017 ... The latter is ranked #18 of the Top 100 non-fiction books of the twentieth century by Modern Library. Andrew Bacevich ... Works by or about Reinhold Niebuhr in libraries (WorldCat catalog). *"The Ironic Element in the American Situation", an excerpt ...
List of tuberculosis cases
Eugene O'Neill. *George Orwell (1903-1950), British author of Nineteen Eighty-Four, Animal Farm and Homage to Catalonia, first ... Calvin College: Christian Classics Ethereal Library. Retrieved 2006-05-08.. *^ "Maria Faustina Kowalska". St. Charles Borromeo ...
English, Adam C. (2016), Christmas: Theological Anticipations, Eugene, Oregon: Wipf & Stock Publishers, ISBN 978-1-4982-3933-2 ... Literature by and about Saint Nicholas in the German National Library catalogue ...
The largest collection of nursery and seed trade catalogs in the U.S. is held at the National Agricultural Library where the ... The use of old varieties maintains diversity in the horticultural gene pool. It may be more appropriate for amateur gardeners ... "Guide to the Collections: Henry G. Gilbert Nursery and Seed Trade Catalog Collection." National Agricultural Library, Special ...
Medical laboratory equipment automates or helps analyze blood, urine, genes, and dissolved gases in the blood. ... "University of Virginia Claude Moore Health Services Library. 2007. Retrieved 16 September 2014.. ...
Subscription or UK public library membership required.) *^ Newlands, John A. R. (20 August 1864). "On Relations Among the ... L. Brown, Theodore; LeMay, Jr., H. Eugene; Bursten, Bruce E.; Burdge, Julia R. (2003). Chemistry: The Central Science (8th ed ...
Gene Wilburn | Open Library
Gene Townsend | Open Library
Books by Gene Townsend, Whaddayah mean leave home and travel for the rest of my life!, The whaddayah-mean leave-home-and-travel ... Open Library is an initiative of the Internet Archive, a 501(c)(3) non-profit, building a digital library of Internet sites and ... New Feature: You can now embed Open Library books on your website! Learn More ... Are you sure you want to remove Gene Townsend from your list? ... by Gene Townsend 1 edition - first published in 1995 Download ...
Gene Library - MeSH - NCBI
All MeSH CategoriesPhenomena and Processes CategoryGenetic PhenomenaGenetic StructuresGene LibraryGenomic LibraryPeptide ... Gene Library. A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell ... National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA ... It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from ...
Eugene Public Library - Wikipedia
Eugene Public Library. Archived from the original (PDF) on April 1, 2010. "About the library." Eugene Public Library. Retrieved ... The Eugene Public Library is a municipal public library in Eugene, Oregon, United States. It has been in four different ... Eugene Public Library was established as a tax-supported entity in 1904. In 1906, Oregons first Carnegie Library was ... "City of Eugene, Eugene Public Library." Shepley Bulfinch. Retrieved September 1, 2010. Adams, Tom (August 12, 2008). "The ...
Gene Sharp - Wikisource, the free online library
Top Picks: Gene Hackman | Chicago Public Library
Gene Hackman knew from childhood that he wanted to be an actor, but his path was not an easy one. Hackman was born 90 years ago ... Gene Hackman knew from childhood that he wanted to be an actor, but his path was not an easy one. Hackman was born 90 years ago ... Gene Hackman has a rich and lasting legacy, and despite his fame, always saw himself as a "regular guy" who maintained his ... Community-contributed content represents the views of the user, not those of Chicago Public Library ...
Awesome Library - Arts - Music - Jazz - Krupa, Gene
Gene Expression: Safari Library
Narrow Roads of Gene Land 1. Narrow Roads of Gene Land 2. Narrow Roads of Gene Land 3. Statistical Methods in Molecular ... Narrow Roads of Gene Land 1. Narrow Roads of Gene Land 2. Narrow Roads of Gene Land 3. Statistical Methods in Molecular ... Safari Library posted by Razib @ 6/09/2007 10:27:00 PM Safari Library ... The Math Gene. Explaining Culture. Origin and Evolution of Cultures. Dawn of Human Culture. The Origins of Virtue Prehistory of ...
Novel gene complex structure determination - Digital Library
University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents ... UNT Libraries Government Documents Department Serving as both a federal and a state depository library, the UNT Libraries ... Gatewood, J.M. Novel gene complex structure determination, report, August 1, 1997; New Mexico. (digital.library.unt.edu/ark:/ ... Digital Library, a digital repository hosted by the UNT Libraries. More information about this report can be viewed below. ...
Gene Localization by in situ Hybridization: FISH - eLS - Ekong - Wiley Online Library
Disadvantages Of Gene In Gattaca - 307 Words | Internet Public Library
... a community of people reliant only on the study of genes definitely has a few flaws. For one, the use of gene identification... ... As the movie Gattaca hints, a community of people reliant only on the study of genes definitely has a few flaws. For one, the ... As the movie showed, people with less healthy genes (such as Vincent), would be unable to reach their dreams. This is unfair! ... More about Disadvantages Of Gene In Gattaca. *. Negatives Of Genetic Engineering. 723 Words , 3 Pages ...
Gene Expression Programming: Library & Information Science Book Chapter | IGI Global
Gene expression programming (GEP) introduced by Candida Ferreira is a descendant of genetic algorithm (GA) and genetic ... "Gene Expression Programming." In Bio-Inspired Computing for Information Retrieval Applications. edited by D.P. Acharjya , and ... "Gene Expression Programming." Bio-Inspired Computing for Information Retrieval Applications, edited by D.P. Acharjya and ... Gene Expression Programming. Baddrud Zaman Laskar (NIT Silchar, India) and Swanirbhar Majumder (NERIST, India). ...
Search Results - 'Roland, Gene' - 5 Results - Digital Library
Group Photograph of Gene Roland and Others] Description: The photograph is of Gene Roland, Keith La Motte, Dwight Carver, Carl ... Photograph of Stan Kenton and Gene Roland] Description: The photograph is of Stan Kenton and Gene Roland (arranger) at a ... Photograph of Stan Kenton and Gene Roland] Description: The photograph is of Stan Kenton and Gene Roland (arranger) at a ... Research @ The UNT Libraries Links and search tools for all of the collections and resources available from UNT. ...
Human gene-splice test considered. - Free Online Library
Human gene-splice test considered. by Science News; Science and technology, general Cancer Research Cancer research Genetic ... The Free Library , Science and Technology , Science and technology, general , Science News , June 25, 1988 ... 2020 https://www.thefreelibrary.com/Human+gene-splice+test+considered.-a06831893. *Chicago style: The Free Library. S.v. Human ... MLA style: "Human gene-splice test considered.." The Free Library. 1988 Science Service, Inc. 04 Dec. ...
Geneticists uncover heart disease gene. (atherosclerosis susceptibility, ATHS) (Brief Article) by Science News; Science and ... The Free Library , Science and Technology , Science and technology, general , Science News , August 8, 1992 ... 2017 https://www.thefreelibrary.com/Geneticists+uncover+heart+disease+gene.-a012535765. *Chicago style: The Free Library. S.v. ... MLA style: "Geneticists uncover heart disease gene.." The Free Library. 1992 Science Service, Inc. 16 Oct. ...
Eugene Field Exhibition - The University of Chicago Library
Eugene Field (1850 - 1895) was a major figure in Chicagos literary and book collecting circles at the time of his death 100 ... "Eugene Field and his Books" marks the centenary of Fields death and the founding of Chicagos Caxton Club. The exhibition, ... and the holdings of the University of Chicago Library, focuses on Field as author, collector and promoter of books and the book ... Library Hours Ask a Librarian Support the Library My Library Account Toggle navigation The University of Chicago Library ...
Eugene Judson Hall - Wikisource, the free online library
David Eugene Price - Wikisource, the free online library
Gene Epstein Archives | EconTalk | Library of Economics and Liberty
Gene Epstein on Gold, the Fed, and Money. EconTalk Episode with Gene Epstein Hosted by Russ Roberts ... Gene Epstein, Barrons economics editor, talks to EconTalk host Russ Roberts about the virtues of the gold standard relative to ... CATEGORIES: Gene Epstein , Government Budgets and Taxation , Law and Institutions , Money, Banking, Monetary Policy ... Gene Epstein Podcast Episodes and Extras. Category Archive with 1 podcast episodes and extras ...
Duplicated Genes Database API Libraries | ProgrammableWeb
The Duplicated Genes Database (DGD) provides a list of co-located and duplicated genes. These genes may be either tandem ... Libraries. The following is a list of libraries from ProgrammableWebs Library Directory that matched your search term. ... or to study the duplication status of a specific gene or group of genes. The SOAP-based DGD API can only use Ensembl gene IDs ... Lists of duplicated genes can be used to analyze the expression of genes from microarray profiling experiments, to characterize ...
Gene Spencer • Library and Information Technology • Ursinus
Gene provides leadership for all areas of Library and Information Technology support on campus, including circulation, ... Gene has also served in a variety of IT and joint Library/IT positions at his alma mater, Bucknell University. As a consultant ... Gene provides leadership for all areas of Library and Information Technology support on campus, including circulation, ... Please feel free to contact Gene with any questions or concerns you might have with information technology or library services ...
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. - PubMed - NCBI
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.. Dietzl G1, Chen D, Schnorrer F, Su KC, ... Our transgenic RNAi library thus opens up the prospect of systematically analysing gene functions in any tissue and at any ... National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA ... Our RNAi transgenes consist of short gene fragments cloned as inverted repeats and expressed using the binary GAL4/UAS system. ...
Cloning thousands of genes for massive protein libraries | EurekAlert! Science News
Until now, this was performed on a one-gene-at-a-time basis, causing a bottleneck. Scientists at Rutgers University-New ... and Harvard Medical School have invented a technology to clone thousands of genes simultaneously and create massive libraries ... Discovering the function of a gene requires cloning a DNA sequence and expressing it. ... Cloning thousands of genes for massive protein libraries. Rutgers University. Journal. Nature Biomedical Engineering. Funder. ...
WikiGenes - Gene Library
Gene context of Gene Library. *Three members of the Jun/AP-1 family have been identified in mouse cDNA libraries: c-Jun, Jun-B ... Anatomical context of Gene Library. *We have screened a cDNA library for gene sequences that are regulated by platelet-derived ... Biological context of Gene Library. *DNA sequence analysis of a class I gene (Q10), which maps to the Qa2,3 locus in the C57BL/ ... Disease relevance of Gene Library. *Phage subclones from the breakpoint region were isolated and used to screen cDNA libraries ...
Pollen and gene flow in fragmented habitats - Kwak - 1998 - Applied Vegetation Science - Wiley Online Library
Pollen and gene flow in fragmented habitats. Authors. *. Manja M. Kwak,. Corresponding author. *Laboratory of Plant Ecology and ... Wiley Online Library. *11. David M. Lowenstein, Kevin C. Matteson, Emily S. Minor, Diversity of wild bees supports pollination ... Shenhua Qian, Waka Saito, Makiko Mimura, Shingo Kaneko, Yuji Isagi, Eri Mizumachi, Akira S. Mori, Asymmetric gene flow and the ... In the absence of stepping stones even short distances between populations act as strong barriers for gene flow. ...
Tranzyme, Univ. of Utah Collaborate to Develop Gene Libraries | Genomeweb
17 - Tranzyme and the University of Utah Genome Center have collaborated to develop gene libraries, the organizations announced ... 17 - Tranzyme and the University of Utah Genome Center have collaborated to develop gene libraries, the organizations announced ... New PacBio Library Prep Method Helps Cover Death Valley Between Short and Long Reads Premium ... Birmingham, Ala.-based Tranzyme will use its genomics platform to help build the libraries as it uses the joint project to ...
P[acman]-generated fruit fly gene 'library': A new research tool
... so that a researcher can choose a gene and find the corresponding clones in the library that cover that gene. Their ... "You can insert a single copy of a gene and rescue a mutation, or do a structure/function analysis of the gene," Bellen said. " ... P[acman]-generated fruit fly gene library: A new research tool. 26.05.2009 ... generated fruit fly gene , artificial chromosome , fruit fly , genetic research , genomic libraries , transgenesis ...
Eugene Field Curbside Holds Pickup | Denver Public Library
Specific Instructions for Eugene Field Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park in ... Specific Instructions for Eugene Field. Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park ... All Denver Public Library locations remain closed except for curbside holds pickup. New holds can now be placed. ... As per City ordinance, we ask all customers to wear face masks when visiting the library for curbside pickup. ...
Eugene Field Curbside Holds Pickup | Denver Public Library
Specific Instructions for Eugene Field Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park in ... Specific Instructions for Eugene Field. Enter library parking lot from Ohio Ave. and follow signs to curbside hold area. Park ... Holds scheduled for curbside pickup will be available at the Eugene Field Branch Library. ... Picking Up: call when you arrive any time during your appointment slot, bring your library card or ID, dont forget your mask. ...
Hacking CRISPR: Patents, Gene Therapy & Embryos | Bioethics Research Library
Trending globally: gene editing experiments with human embryos. On April 8, news broke that the second paper documenting CRISPR ... "gene editing" tools are not only (relatively) quicker, cheaper, and easier than any previous genetic engineering method, but ... expressed by these authors and blogs are theirs and do not necessarily represent that of the Bioethics Research Library and ...
GenomeLuen YangThousands of genesCatalog20,000 genesExpression2020MicroarrayEmbryosNiobrara County LibraryDrosophilaSearchTarget genesGenomesApoptosisMammalianRetroviralCollectionsContentResearchersField Branch Library20172000RegulationCloneGenomic LibraryScientistsCLONINGAntibodyRNAiChromosomeRRNAMechanismComplex10thMOLECULARProteinExperimentsOntologyCombinatorialSilencingRegister-GuardClonesAuthorSpecific geneClustersHumanCity CouncilWorld'sNewly discoveredManuscript LibraryMainTherapySynthesisTherapeutic1959
- A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. (nih.gov)
- With the availability of complete genome sequences and the introduction of RNA-mediated gene interference (RNAi), systematic reverse genetic screens are now also possible. (nih.gov)
- Until now, such genome-wide RNAi screens have mostly been restricted to cultured cells and ubiquitous gene inactivation in Caenorhabditis elegans. (nih.gov)
- Here we report the generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact organism. (nih.gov)
- We generated 22,270 transgenic lines, covering 88% of the predicted protein-coding genes in the Drosophila genome. (nih.gov)
- They can now take a genome sequence (or many of them) and make a protein library for screening with unprecedented speed, cost-effectiveness and precision, allowing rapid discovery of potentially beneficial biomolecules from a genome. (eurekalert.org)
- SAN FRANCISCO, Oct. 17 - Tranzyme and the University of Utah Genome Center have collaborated to develop gene libraries, the organizations announced Wednesday. (genomeweb.com)
- The center is currently involved in the Environmental Genome Project for the evaluation of sequence variation in environmental response genes as well as the mouse and rat genome projects. (genomeweb.com)
- In Genome Research this week: IPAFinder method to detect intronic polyadenylation, influence of structural variants on gene expression, and more. (genomeweb.com)
- Using a specially adapted tool called P[acman], a collaboration of researchers led by Baylor College of Medicine has established a library of clones that cover most of the genome of Drosophila melanogaster (fruit fly) and should speed the pace of genetic research. (innovations-report.com)
- Following infection, the H-19B genome with its toxin genes can integrate into the bacterial chromosome as a prophage, in this way transferring stx genes to a new host. (usda.gov)
- Threadgill developed the idea for the CC in order to harness the power of so-called whole genome studies that examine all genes at once instead of subsets of genes. (healthcanal.com)
- refs) Vector-based siRNA delivery strategies for high-throughput screening of novel target genes Meihong Chen †, Quan Du ‡, Hong-Yan Zhang ‡, Claes Wahlestedt ‡§, and Zicai Liang ‡* † Chinese Human Genome Center North, Beijing, 100176, China and Institute. (libpubmedia.co.uk)
- The research team created a new genome-wide CRISPR library of 88,000 guide RNAs that enabled them to switch off each of the 20,000 genes from mouse Th2 cells. (b-s-h.org.uk)
- They mimicked an infection in cultured Th2 cells and looked at how switching off each single gene in the genome affected the activation or differentiation. (b-s-h.org.uk)
- The Avatar's adventures continue right where the TV series left off, in this beautiful oversized hardcover of The Promise, from Airbender creators Michael Dante DiMartino and Bryan Konietzko and Eisner and Harvey Award winner Gene Luen Yang! (bookdepository.com)
- Gene Luen Yang won two Eisner Awards for Superman Smashes the Klan (Best Publication for Kids, and Best Adaptation from Another Medium) and one for Dragon Hoops (Best Publication for Teens). (slj.com)
- Jillian Tamaki won Best Publication for Early Readers with Our Little Kitchen and Gene Luen Yang earned three Eisners, two for Superman Smashes the Klan (Best Publication for Kids, and Best Adaptation from Another Medium) and one for Dragon Hoops (Best Publication for Teens). (slj.com)
Thousands of genes4
- Scientists at Rutgers University-New Brunswick in collaboration with Johns Hopkins University and Harvard Medical School have invented a technology to clone thousands of genes simultaneously and create massive libraries of proteins from DNA samples, potentially ushering in a new era of functional genomics. (eurekalert.org)
- The DNA mciroarray gene data is in the expression levels of thousands of genes for a small amount of samples. (igi-global.com)
- Recent advances in microarray technology have enabled the measurement of the simultaneous expression of thousands of genes under multiple experimental conditions. (igi-global.com)
- It takes thousands of genes to build the photosynthetic machinery that plants need to harness sunlight for growth. (brightsurf.com)
- Microarray analysis will allow us to determine levels of gene expression for 20,000 genes simultaneously, and provide information as to the mechanism by which probiotics mediate the Salmonella-reducing effects. (usda.gov)
- Lists of duplicated genes can be used to analyze the expression of genes from microarray profiling experiments, to characterize the genomic content of a specific chromosomal region, or to study the duplication status of a specific gene or group of genes. (programmableweb.com)
- In September, Tranzyme made a deal with Active Pass Pharmaceuticals of Vancouver to combine technologies in an effort to study gene expression and bolster their drug discovery programs. (genomeweb.com)
- Efficient Retrieval Technique for Microarray Gene Expression. (igi-global.com)
- Microarray techniques have been successfully used to investigate useful information for cancer diagnosis at the gene expression level, the true integration of existing methods into day-to-day clinical practice is very challenging. (igi-global.com)
- Mammalian expression cloning methods that permit multiple rounds of selection and enrichment have been extremely successful in identifying cDNA clones from plasmid libraries that correspond to unknown receptors or ligands ( 7 ). (pnas.org)
- Unfortunately, the requirement for large T antigen-driven replication of plasmid libraries in mammalian cells has severely limited adaptation of expression cloning methods to functional screens with only highly transfectable cell lines. (pnas.org)
- The regulation of P450 gene expression has been well documented in experimental animals, but at present there is very little information available about the regulation of human P450 genes, particularly in extra-hepatic tissues. (bl.uk)
- Regulation of P450 expression by a range of xenobiotics, known to have profound effects on the expression of rodent P450 genes, has been studied in a mouse model and in cultured cells. (bl.uk)
- TCPOBOP was shown to be equally effective at influencing human P450 gene expression and, in most cases, the patterns of gene regulation observed in experimental animals were also seen in the human tumours. (bl.uk)
- Why do you need gene expression library by the way? (helico.com)
- Outputs will include quantitation of Salmonella reduction, lists of differentially expressed genes, and output from gene expression analyses. (usda.gov)
- 32 microarray slides total (16 per time point) will be hybridized and scanned to provide gene expression levels for 21,120 genes. (usda.gov)
- In particular, the role of specific phage-encoded functions in stx expression will be studied by directing mutations to genes encoding functions critical in phage growth and gene expression. (usda.gov)
- Construction of the SPL was performed in a vector (pSIP409) previously developed for high-level, inducible gene expression in L. plantarum and Lactobacillus sakei. (nofima.no)
- The SPIL enables fine-tuning of stable gene expression for various applications in L. plantarum. (nofima.no)
- Regulation of aldose reductase gene expression in renal cortex and medulla of rats. (bl.uk)
- Non-parallelism of islet amyloid polypeptide (amylin) and insulin gene expression in rats islets following dexamethasone treatment. (bl.uk)
- refs) PCR-based expression analysis and identification of microRNAs David P Lu †, Rebecca L Read †, David T Humphreys ‡, Fiona M Battah †, David I K Martin ‡ and John E J Rasko †§* † Gene. (libpubmedia.co.uk)
- We will discuss how to avoid doing a BLAST search and still obtain the relevant information and Blast Variations for structure/function search The Gene Expression Omnibus (GEO) is a public repository that archives and freely distributes microarray, next-generation sequencing, and other forms of high-throughput functional genomic data. (harvard.edu)
- Second, deregulation of the expression of septin family genes in hematological cancers can be observed either with or without the concomitant presence of MLL gene fusions. (begellhouse.com)
- Ann Arbor District Library , 2020. (aadl.org)
- From the microarray gene data, the process of extracting the required knowledge remains an open challenge. (igi-global.com)
- The technique like LPP and PCA is chosen for the dimensionality reduction for efficient retrieval of microarray gene data. (igi-global.com)
- An application of microarray gene data is included with classification by SVM. (igi-global.com)
- Selected differentially expressed genes will be selected for qRT-PCR analysis to confirm the microarray data. (usda.gov)
- Researchers will alter the genes in donated embryos, then destroy them at age seven days. (georgetown.edu)
Niobrara County Library1
- The Niobrara County Library Foundation, Inc. accepts online donations. (niobraracountylibrary.org)
- Our transgenic RNAi library thus opens up the prospect of systematically analysing gene functions in any tissue and at any stage of the Drosophila lifespan. (nih.gov)
- The following is a list of libraries from ProgrammableWeb's Library Directory that matched your search term. (programmableweb.com)
- Although there many different interpretations of the word "library" among software developers, ProgrammableWeb adheres to a specific definition so as to clearly distinguish libraries from SDKs and frameworks in a way that will facilitate clean search results. (programmableweb.com)
- These arrayed sets contain all shRNA from the human TRC lentiviral shRNA library that target genes falling under specific GO terms. (horizondiscovery.com)
- The next phase, which is underway, is to improve the cloning process, build libraries and discover therapeutic proteins found in our genomes, Parekkadan said. (eurekalert.org)
- In distantly related genomes, clusters of homologous gene pairs are evidence of candidate homologous regions. (wolfram.com)
- We present additional simulation results indicating the best choice of parameter values for data analysis in genomes of various sizes and illustrate the utility of our methods by applying them to gene clusters recently reported in the literature. (wolfram.com)
- In 15 papers published Feb. 16 in the Genetics Society of America journals Genetics and G3:Genes/Genomes/Genetics , researchers from North Carolina State University, the University of North Carolina at Chapel Hill, The Jackson Laboratory and other universities and labs across the globe highlight a new genetic resource that could aid development of more effective treatments for any number of human diseases. (healthcanal.com)
- Three of the genes, RelA, cellular FLICE-like inhibitory protein (c-FLIP), and a dominant-negative mutant of TNF receptor 1 arising through CPR afforded strong protection against apoptosis. (pnas.org)
- Two of the genes identified, Dbs and Fas-associated death domain protein (FADD), previously identified as a proapoptotic molecule, afforded partial protection against TNFα-induced apoptosis. (pnas.org)
- The proposed trial combines recent advances in cancer therapy -- spearheaded by Steven A. Rosenberg at the National Cancer Institute -- with work by National Heart, Blood and Lung Institute researcher W. French Anderson, a pioneer in the art of inserting foreign genes into mammalian cells. (thefreelibrary.com)
- Genes regulating responses in mammalian cells are often difficult to identify by functional cloning strategies limited to a single round of selection. (pnas.org)
- Retroviral cDNA libraries have been used in a number of functional screens to identify unique mammalian genes that regulate cellular responses ( 1 - 6 ). (pnas.org)
- To ensure that positive cDNA clones are successfully isolated from complex libraries in a single round of selection, functional screens using mammalian cells have been restricted to cellular assays in which the background of false-positive cells meeting a selection criterion is low, typically 1 in 10 5 false-positive cells. (pnas.org)
- Here we describe an alternative method called cyclical packaging rescue (CPR) that uses direct repackaging of retroviral RNAs into new infectious virions to identify genes regulating functional responses in mammalian cells (Fig. 1 A ). In CPR, stably integrated helper-free retroviral libraries are recovered rapidly from mammalian cells as infectious helper-free retroviral supernatants 24 h after infection with adenoviruses expressing retroviral gag-pol and env genes. (pnas.org)
- These results suggest that CPR is a rapid and versatile approach that should facilitate functional analysis of gene function in mammalian cells. (pnas.org)
- Sry is believed to act as a switch in the complex gene mechanism that diverts mammalian development from its usual course - the female - to the male. (sciencephoto.com)
- Here we describe a strategy, cyclical packaging rescue (CPR), which allows rapid recovery and retransmission of retroviral cDNA libraries. (pnas.org)
- When performed in concert with selection using functional assays, cDNAs regulating functional responses can be identified by enrichment through multiple rounds of retroviral library recovery and retransmission. (pnas.org)
- Scientists have created the first retroviral CRISPR-Cas9 gene editing library to examine the regulation of mouse T cells, it was announced last week. (b-s-h.org.uk)
- Dr Xi Chen, joint first author from the Wellcome Sanger Institute, said while previous studies have mostly examined one gene at time, the development of a retroviral CRISPR library has enabled them to knock-out each individual gene in mouse T helper cells for the first time. (b-s-h.org.uk)
- The fonds was donated to University of Manitoba Archives & Special Collections by Gene Telpner in 1991. (umanitoba.ca)
- The Library of Congress maintains one of the largest and most diverse collections of scientific and technical information in the world. (loc.gov)
- The Science, Technology and Business Division provides reference and bibliographic services and develops the general collections of the Library in all areas of science, technology, business and economics, with the exception of clinical medicine and technical agriculture, which are the subject specialties of the National Library of Medicine and the National Agricultural Library. (loc.gov)
- Human and mouse clone collections are compiled into libraries for gene families and pathways. (horizondiscovery.com)
- Gene Ontology (GO) annotated collections targeting all genes in a particular gene family or biological pathway have been created from the human TRC lentiviral shRNA library. (horizondiscovery.com)
- A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. (horizondiscovery.com)
- With methods developed by Anderson, researchers can use a retrovirus to insert into TIL cells a gene that confers resistance to the antibiotic neomycin. (thefreelibrary.com)
- The researchers reported in the Jan. 15 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES that this inheritance pattern suggests that the ATHS gene lies on chromosome 19, near the gene for the LDL receptor. (thefreelibrary.com)
- Learning more about these interactions could help researchers tease out links between certain genes and certain diseases, for example. (healthcanal.com)
- And yet, researchers don't know exactly how these genes work. (brightsurf.com)
- Now a team led by Princeton University researchers has constructed a public "library" to help researchers to find out what each gene does. (brightsurf.com)
- Unlocking the role of each gene could allow researchers to engineer plants to grow more quickly, potentially meeting future world food needs. (brightsurf.com)
- The ability to observe a Chlamy cell with just one defective gene among all the other functioning genes allows researchers to figure out what that gene does. (brightsurf.com)
- The library enables researchers to test multiple mutant Chlamy strains at once because each mutation is labeled with a unique "DNA barcode. (brightsurf.com)
- We have partnered with the TRC to make the shRNA libraries, which are available to researchers worldwide, and to compile shRNA libraries for popular gene families and pathways. (horizondiscovery.com)
- We developed a prototype functional alerting system for researchers based on the GeneRIFs, and a strategy to find all of the literature related to genes. (nih.gov)
- Researchers working in fields such as protein engineering, discovery biology, structural biology, synthetic biology, antibody engineering, and enzyme engineering choose GENEWIZ's Synthetic DNA Libraries because of our wide range of library types, reliable service, and best-in-class consultation from our Ph.D.-level customer support scientists. (genewiz.com)
Field Branch Library1
- Holds scheduled for curbside pickup will be available at the Eugene Field Branch Library . (denverlibrary.org)
- The maps and much more detail on the land supply, demand and capacity analysis are included in the Envision Eugene Employment Land Supply Study and Residential Land Supply Study , both appendices to the 2017 adopted Envision Eugene Comprehensive Plan . (eugene-or.gov)
- A model was developed, using human tumours grown as xenografts in immune deficient mice, in which the in vivo regulation of human P450 genes could be examined. (bl.uk)
- RNAi2011: Gene Regulation by Small RNAs, the 6 th international Oxford RNAi conference, was held at St Hilda's College, University of Oxford (UK), between 29 and 31 March, 2011. (libpubmedia.co.uk)
- They found many genes involved in the regulation of Th2 development, and identified the transcription factor PPARG as being particularly important. (b-s-h.org.uk)
- This has not only allowed us to identify genes involved in the regulation of Th2 cells but could also be used to study other mouse immune cells and their regulation. (b-s-h.org.uk)
- We examined the bacterial composition of chlorinated drinking water using 16S rRNA gene clone libraries derived from RNA and DNA extracted from twelve water samples collected in three different months (June, August, and September of 2007). (epa.gov)
- It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences. (nih.gov)
- Moreover, the initial 30-day culture period will give scientists a chance to test the gene-altered cells for unusual changes or vira contaminants -- concerns that have been unresolved in previous gene therapy proposals -- before injecting them into patients. (thefreelibrary.com)
- With competitive pricing and project completion in as little as 11 days, discover why hundreds of scientists from leading research facilities choose GENEWIZ for their partially (NNK) or completely randomized (NNN) nucleotide libraries. (genewiz.com)
- Discovering the function of a gene requires cloning a DNA sequence and expressing it. (eurekalert.org)
- The team also reported the capture and cloning of the first protein library, or suite of proteins, from a human microbiome sample. (eurekalert.org)
- Molecular cloning of a group of mouse pancreatic islet beta-cell-related genes by random cDNA sequencing. (bl.uk)
- Isogenica is currently commercialising the Colibra™ technology for ratio controlled protein and antibody libraries based on a partly automated process. (businesswire.com)
- Isogenica's CIS display technology is an in vitro display technology that allows the rapid generation of polypeptide and antibody libraries from which it is possible to select lead molecules with high affinity and specificity for most targets. (businesswire.com)
- Gene conversion has been shown to occur both intrachromosomally on the Y chromosome, and between the X and the Y chromosomes (Cruciani et al. (bl.uk)
- 2009). This study has used interspecies sequence comparisons to identify regions of the Y chromosome which are likely to be undergoing gene conversion. (bl.uk)
- This study suggests that gene conversion between Y chromosome paralogs is conservative of the ancestral sequence via an unknown mechanism and that conservative gene conversion is not limited to genic regions of the Y chromosome. (bl.uk)
- The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. (asm.org)
- Published online in the journal Cell , the work focuses on the complex control mechanism, describing how different genes are involved in both activation and development of T helper cells. (b-s-h.org.uk)
- Complex interactions between large numbers of genes frequently govern traits and behavior. (healthcanal.com)
- This remained the main library building until it was moved to its current location at West 10th Avenue and Charnelton Street in 2002. (wikipedia.org)
- On Wednesday , April 29 , at 6:30 p.m. at the Central Library , 14 W. 10th St., Gene Healy presents his book The Cult of the Presidency: America's Dangerous Devotion to Executive Power . (kclibrary.org)
- Free parking is available in the Library District Parking Garage located at 10th and Baltimore. (kclibrary.org)
- In a report in the current online issue of the journal Nature Methods, Dr. Hugo Bellen (http://flypush.imgen.bcm.tmc.edu/lab/), a professor of molecular and human genetics at BCM and a Howard Hughes Medical Institute investigator, and his colleagues describe the new libraries. (innovations-report.com)
- Molecular model of catabolite gene activator protein (CAP, pink and green) bound to a molecule of deoxyribonucleic acid (DNA, across top). (sciencephoto.com)
- One of the newly identified genes is CPL3, which is thought to play a role in accumulating the protein "parts" of the photosynthetic machinery. (brightsurf.com)
- Gene Indexing links articles about the basic biology of a gene or protein within eight model organisms to a specific record in the NLM's LocusLink database of gene products. (nih.gov)
- Alanine is usually the preferred choice for a scanning library because substitution with alanine eliminates side-chain interactions while retaining the structural integrity of the protein because it is the smallest chiral amino acid. (genewiz.com)
- A truncation library of mutants is created by trimming amino acids systematically from either the N- or C-terminus (or both), of a protein around a pre-defined core region. (genewiz.com)
- Experiments in animals suggest such "gene therapy" may prove useful against thousands of hereditary diseases, but a variety of medical and ethical concerns have stalled the start of human experiments. (thefreelibrary.com)
- Dr. Kathy Niakan, the researcher who will be performing the experiments, said, "We would really like to understand the genes needed for a human embryo to develop successfully into a healthy baby. (georgetown.edu)
- Gene Ontology (biological process) analysis will be completed using the Bioresource for Array Genes database and Pathway Miner databases. (usda.gov)
- GO annotations are applied to genes by curators within the Gene Ontology Consortium and do not originate from us. (horizondiscovery.com)
- Consult the Gene Ontology website for details. (horizondiscovery.com)
- This will allow both parties to efficiently optimise the assembly of genes from smaller DNA fragments and generate combinatorial libraries of genetic mutations for application in the field of synthetic biology. (businesswire.com)
- Learn how the trimer-controlled combinatorial DNA library synthesis approach provides full control over amino acid distribution and offers higher efficiency for downstream screening. (genewiz.com)
- A combinatorial library allows you to create a library of mutants by using different genetic elements in multiple combinations to determine the most beneficial combination. (genewiz.com)
- You can obtain a combinatorial library using traditional partially randomized (NNK/NNS) or completely randomized (NNN) approaches. (genewiz.com)
- Kostas Kostarelos (University of London, UK) described how carbon nanotubes, which are internalised by the cell without apparent damage to cellular structure, could be chemically functionalised and combined with siRNAs to form gene-silencing complexes that are effectively delivered to target cells. (libpubmedia.co.uk)
- The Register-Guard (Eugene, Oregon). (wikipedia.org)
- Venken adapted the P[acman] vector to create genomic libraries, so that a researcher can choose a gene and find the corresponding clones in the library that cover that gene. (innovations-report.com)
- The exhibition, which includes manuscripts, correspondence, limited editions and other materials from the private collection of Frank J. Piehl (Ph.D., 1952) and the holdings of the University of Chicago Library, focuses on Field as author, collector and promoter of books and the book arts. (uchicago.edu)
- In one of the 15 papers, Threadgill and corresponding author Dr. Francis S. Collins, director of the National Institutes of Health, identify key genes involved in red and white blood cell counts and red blood cell volume. (healthcanal.com)
- Xiaobo Li, the study's first author, was a postdoctoral researcher at Princeton when the team completed the library. (brightsurf.com)
- Contributors: Gene H. Bell-Villada - Author. (questia.com)
- You and other family members can then be tested for that specific gene change. (wellspan.org)
- Demonstrating the statistical significance of such gene clusters is an essential component of comparative genomic analyses. (wolfram.com)
- However, currently there are no practical statistical tests for gene clusters that model the influence of the number of homologs in each gene family on cluster significance. (wolfram.com)
- Genes in clusters exhibiting specific and reciprocal responses to SE and probiotic will be studied further. (usda.gov)
- As the holiday season approaches, the Eugene City Council was feeling generous at its Nov. 23 meeting. (eugeneweekly.com)
- In 2005, the Eugene City Council adopted and applied the /WR Water Resources Conservation Overlay Zone measures to implement statewide planning Goal 5 inside Eugene's city limits. (eugene-or.gov)
- The Linda Hall Library is the world's foremost independent research library devoted to science, engineering, and technology. (lindahall.org)
- Using the library, the team identified 303 genes associated with photosynthesis including 21 newly discovered genes with high potential to provide new insights into this life-sustaining biological process. (brightsurf.com)
- This thesis can be viewed in person at the Mudd Manuscript Library . (princeton.edu)
- found 1 item where the library's type is Public and the date implemented is 2008 and including only main facilities and the library automation system used is Genesis G3 and the library automation system previously installed was Athena . (librarytechnology.org)
- This piece hangs on a two-story high blue tiled wall just inside the main entrance on the second level of the new library. (easternct.edu)
- T. Yamamoto, Y. Tsunetsugu-Yokota, Prospects for the therapeutic application of lentivirus-based gene therapy to HIV-1 infection. (horizondiscovery.com)
- Gene Therapy Strategies for Retinitis Pigmentosa. (tufts.edu)
- First, some septins (SEPT2, SEPT5, SEPT6, SEPT9, and SEPT11) have been repeatedly identified as in-frame fusion partners of the MLL gene in de novo and therapy-related myeloid neoplasia, in both children and adults. (begellhouse.com)
- This support from the TSB follows the successful development by Isogenica of its Colibra™ technology for the commercial synthesis of ratio controlled gene libraries using a patent protected methodology discovered by Dr Anna Hine of Aston University which was also supported by TSB funding. (businesswire.com)
- Although the current proposal simply calls for inserting a marker gene, later proposals may seek approval for the insertion into TIL cells of genes that code for the production of potentially therapeutic factors such as interleukin-2 or tumor necrosis factor, Blaese says. (thefreelibrary.com)
- In 1959, a new library building opened at the corner of West 13th Avenue and Olive Street. (wikipedia.org)