A triterpene that derives from the chair-boat-chair-boat folding of 2,3-oxidosqualene. It is metabolized to CHOLESTEROL and CUCURBITACINS.
An NADPH-dependent P450 enzyme that plays an essential role in the sterol biosynthetic pathway by catalyzing the demethylation of 14-methyl sterols such as lanosterol. The enzyme acts via the repeated hydroxylation of the 14-methyl group, resulting in its stepwise conversion into an alcohol, an aldehyde and then a carboxylate, which is removed as formic acid. Sterol 14-demethylase is an unusual cytochrome P450 enzyme in that it is found in a broad variety of organisms including ANIMALS; PLANTS; FUNGI; and protozoa.
Enzymes of the isomerase class that catalyze the transfer of acyl-, phospho-, amino- or other groups from one position within a molecule to another. EC 5.4.
Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)
A steroid of interest both because its biosynthesis in FUNGI is a target of ANTIFUNGAL AGENTS, notably AZOLES, and because when it is present in SKIN of animals, ULTRAVIOLET RAYS break a bond to result in ERGOCALCIFEROL.
Cholestadiene derivatives containing a hydroxy group anywhere in the molecule.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Steroids with methyl groups at C-10 and C-13 and a branched 8-carbon chain at C-17. Members include compounds with any degree of unsaturation; however, CHOLESTADIENES is available for derivatives containing two double bonds.
Broad spectrum antifungal agent used for long periods at high doses, especially in immunosuppressed patients.
An intermediate in the synthesis of cholesterol.
A superfamily of hundreds of closely related HEMEPROTEINS found throughout the phylogenetic spectrum, from animals, plants, fungi, to bacteria. They include numerous complex monooxygenases (MIXED FUNCTION OXYGENASES). In animals, these P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs (BIOTRANSFORMATION). They are classified, according to their sequence similarities rather than functions, into CYP gene families (>40% homology) and subfamilies (>59% homology). For example, enzymes from the CYP1, CYP2, and CYP3 gene families are responsible for most drug metabolism.
Compounds that specifically inhibit STEROL 14-DEMETHYLASE. A variety of azole-derived ANTIFUNGAL AGENTS act through this mechanism.
An anticholesteremic agent that inhibits sterol biosynthesis in animals.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
An imidazole antifungal agent that is used topically and by intravenous infusion.
Enzymes that catalyze the reversible reduction of alpha-carboxyl group of 3-hydroxy-3-methylglutaryl-coenzyme A to yield MEVALONIC ACID.
A class of organic compounds known as STEROLS or STEROIDS derived from plants.
Five membered rings containing a NITROGEN atom.
A species of METHYLOCOCCUS which forms capsules and is capable of autotrophic carbon dioxide fixation. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
Derivatives of the saturated steroid cholestane with methyl groups at C-18 and C-19 and an iso-octyl side chain at C-17.
The second enzyme in the committed pathway for CHOLESTEROL biosynthesis, this enzyme catalyzes the first oxygenation step in the biosynthesis of STEROLS and is thought to be a rate limiting enzyme in this pathway. Specifically, this enzyme catalyzes the conversion of SQUALENE to (S)-squalene-2,3-epoxide.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
A mushroom, Ganoderma lucidum, of the POLYPORALES order of basidiomycetous fungi. It has long been used in traditional Chinese medicine in various forms.
Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues.
A plant genus of the family ARACEAE. Members contain acrid calcium oxalate and LECTINS. Polynesians prepare the root into poi. Common names of Taro and Coco Yam (Cocoyam) may be confused with other ARACEAE; XANTHOSOMA; or with common yam (DIOSCOREA).
Triazole antifungal agent that is used to treat oropharyngeal CANDIDIASIS and cryptococcal MENINGITIS in AIDS.
Enzymes that catalyze the transposition of double bond(s) in a steroid molecule. EC 5.3.3.
A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.
Chemicals that kill or inhibit the growth of fungi in agricultural applications, on wood, plastics, or other materials, in swimming pools, etc.
A unicellular budding fungus which is the principal pathogenic species causing CANDIDIASIS (moniliasis).
A flavoprotein that catalyzes the reduction of heme-thiolate-dependent monooxygenases and is part of the microsomal hydroxylating system. EC 1.6.2.4.
Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
A genus of ascomycetous FUNGI, family Pneumocystidaceae, order Pneumocystidales. It includes various host-specific species causing PNEUMOCYSTIS PNEUMONIA in humans and other MAMMALS.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A pulmonary disease in humans occurring in immunodeficient or malnourished patients or infants, characterized by DYSPNEA, tachypnea, and HYPOXEMIA. Pneumocystis pneumonia is a frequently seen opportunistic infection in AIDS. It is caused by the fungus PNEUMOCYSTIS JIROVECII. The disease is also found in other MAMMALS where it is caused by related species of Pneumocystis.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A multisubunit enzyme complex containing CYTOCHROME A GROUP; CYTOCHROME A3; two copper atoms; and 13 different protein subunits. It is the terminal oxidase complex of the RESPIRATORY CHAIN and collects electrons that are transferred from the reduced CYTOCHROME C GROUP and donates them to molecular OXYGEN, which is then reduced to water. The redox reaction is simultaneously coupled to the transport of PROTONS across the inner mitochondrial membrane.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
Tellurium. An element that is a member of the chalcogen family. It has the atomic symbol Te, atomic number 52, and atomic weight 127.60. It has been used as a coloring agent and in the manufacture of electrical equipment. Exposure may cause nausea, vomiting, and CNS depression.
The first committed enzyme of the biosynthesis pathway that leads to the production of STEROLS. it catalyzes the synthesis of SQUALENE from farnesyl pyrophosphate via the intermediate PRESQUALENE PYROPHOSPHATE. This enzyme is also a critical branch point enzyme in the biosynthesis of ISOPRENOIDS that is thought to regulate the flux of isoprene intermediates through the sterol pathway.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
Plant steroids ubiquitously distributed throughout the plant kingdom. They play essential roles in modulating growth and differentiation of cells at nanomolar to micromolar concentrations.
Steroidal compounds in which one or more carbon atoms in the steroid ring system have been substituted with non-carbon atoms.
Six-carbon alicyclic hydrocarbons.
The gene ERG11 controls lanosterol creation. Lanosterol is found within the yeast plasma membrane. It is a class of ... Terconazole binds to the heme iron component on the cytochrome P450 enzyme lanosterol of fungi, also known as CYP3A4. ... Terconazole targets fungi specifically since humans do not use lanosterol in this pathway. This process does not affect all ... This occurs by transforming lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-β-ol. This stops respiration by prohibiting ...
Gene. 567 (2): 164-72. doi:10.1016/j.gene.2015.04.082. PMID 25956376. "nhr-1 (gene)". WormBase : Nematode Information Resource ... "Identification of farnesoid X receptor beta as a novel mammalian nuclear receptor sensing lanosterol". Molecular and Cellular ... when bound to their cognate nuclear receptors is normally to upregulate gene expression. This stimulation of gene expression by ... "sex-1 (gene)". WormBase : Nematode Information Resource. Otte K, Kranz H, Kober I, Thompson P, Hoefer M, Haubold B, Remmel B, ...
Other gene mutations are also known to contribute to development of resistance. C. glabrata develops resistance by up ... lanosterol. Development of resistance to one azole in this way will confer resistance to all drugs in the class. Another ... resistance occurs by way of mutations in the ERG11 gene, which codes for 14α-demethylase. These mutations prevent the azole ... regulating CDR genes, and resistance in C. krusei is mediated by reduced sensitivity of the target enzyme to inhibition by the ...
In humans, squalene epoxidase is encoded by the SQLE gene. Squalene monooxygenase (SqMO) was formerly referred to as squalene ... 25-epoxylanosterol by lanosterol synthase. Model organisms have been used in the study of SQLE function. A conditional knockout ... "Entrez Gene: SQLE squalene epoxidase". Nagai M, Sakakibara J, Wakui K, Fukushima Y, Igarashi S, Tsuji S, Arakawa M, Ono T (Aug ... Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Lu Y, Dollé ME, Imholz S, van 't Slot R, Verschuren ...
Zhang is particularly known for his work on lanosterol, stem cell research (particularly limbal stem cells), gene editing, and ... In 2015, Zhang discovered that lanosterol can be used in eyedrop form to help prevent cataracts. In 2019, Zhang took on a new ... July 2015). "Lanosterol reverses protein aggregation in cataracts". Nature. 523 (7562): 607-611. doi:10.1038/nature14650. PMID ... "Gene and mutation independent therapy via CRISPR-Cas9 mediated cellular reprogramming in rod photoreceptors". Cell Res. 27 (6 ...
Lanosterol 14-alpha demethylase from Cunninghamella elegans on www.uniprot.org Cha, C. J.; Kim, S. J.; Kim, Y. H.; Stingley, R ... Wang, R. F.; Khan, A. A.; Cao, W. W.; Cerniglia, C. E. (2000). "Cloning, sequencing, and expression of the gene encoding ... C. elegans also possesses a lanosterol 14-alpha demethylase, another enzyme in the cytochrome P450 family. Cunninghamella ... "The expression of cytochrome P-450 and cytochrome P-450 reductase genes in the simultaneous transformation of corticosteroids ...
The bacterium has a large genome by the standards of other PVC bacteria, around 9 megabases, and contains about 8,000 genes. It ... The sterols identified in the bacterium, lanosterol and parkeol, are relatively simple compared to eukaryotic sterols; as ... A study of indels in protein-coding genes of the PVC grouping identified a number of biochemical pathways with unusually high ... Transcription and translation of genes have been reported to occur in spatially segregated locations within the cell, which is ...
In humans, the PGRMC1 protein is encoded by the PGRMC1 gene. The sole biochemical function of PGRMC1 is heme-binding. PGRMC1 ... for a critical step in cholesterol synthesis in which the P450 protein Erg11/Cyp51 removes a methyl group from lanosterol. ... Yeast cells lacking the DAP1 gene are sensitive to DNA damage, and heme-binding is essential for damage resistance. Dap1 is ... As a result, yeast cells lacking the DAP1 gene are highly sensitive to antifungal drugs This function is conserved between the ...
... of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol ...
Multidrug-resistance (MDR) genes can also play a role in reducing cellular levels of the drug. As azole antifungals all act at ... This enzyme participates in the sterol biosynthesis pathway that leads from lanosterol to ergosterol. Lower doses of ... Sanglard D, Ischer F, Monod M, Bille J (February 1997). "Cloning of Candida albicans genes conferring resistance to azole ... antifungal agents: characterization of CDR2, a new multidrug ABC transporter gene". Microbiology. 143 ( Pt 2) (Pt 2): 405-16. ...
Mice have genes for 101 CYPs, and sea urchins have even more (perhaps as many as 120 genes). Most CYP enzymes are presumed to ... This interrupts the conversion of lanosterol to ergosterol, a component of the fungal cell membrane. (This is useful only ... Humans have 57 genes and more than 59 pseudogenes divided among 18 families of cytochrome P450 genes and 43 subfamilies. This ... The convention is to italicise the name when referring to the gene. For example, CYP2E1 is the gene that encodes the enzyme ...
In humans, lanosterol synthase is encoded by the LSS gene. In eukaryotes, lanosterol synthase is an integral monotopic protein ... Baker CH, Matsuda SP, Liu DR, Corey EJ (Aug 1995). "Molecular cloning of the human gene encoding lanosterol synthase from a ... Young M, Chen H, Lalioti MD, Antonarakis SE (May 1996). "The human lanosterol synthase gene maps to chromosome 21q22.3". Human ... Young, Michele; Chen, Haiming; Lalioti, Maria D.; Antonarakis, Stylianos E. (May 1996). "The human lanosterol synthase gene ...
1 subfamily, 1 gene. CYP46A1 CYP51. cholesterol biosynthesis. 1 subfamily, 1 gene, 3 pseudogenes. CYP51A1 (lanosterol 14-alpha ... 2 subfamilies, 2 genes. CYP7A1, CYP7B1 CYP8. varied. 2 subfamilies, 2 genes. CYP8A1 (prostacyclin synthase), CYP8B1 (bile acid ... 3 subfamilies, 3 genes. CYP26A1, CYP26B1, CYP26C1 CYP27. varied. 3 subfamilies, 3 genes. CYP27A1 (bile acid biosynthesis), ... 3 subfamilies, 3 genes, 1 pseudogene. CYP1A1, CYP1A2, CYP1B1 CYP2. drug and steroid metabolism. 13 subfamilies, 16 genes, 16 ...
... lanosterol 14-demethylase, lanosterol 14alpha-demethylase, and sterol 14alpha-demethylase. This enzyme participates in ... Leroux P, Albertini C, Gautier A, Gredt M, Walker AS (July 2007). "Mutations in the CYP51 gene correlated with changes in ... Although lanosterol 14α-demethylase is present in a wide variety of organisms, this enzyme is studied primarily in the context ... Aoyama Y, Yoshida Y (1992). "The 4 beta-methyl group of substrate does not affect the activity of lanosterol 14 alpha- ...
MF59 is capable of switching on a number of genes that partially overlap with those activated by other adjuvants. How these ... It then undergoes an enzyme-catalysed cyclisation to produce lanosterol, which can be elaborated into other steroids such as ... Click on genes, proteins and metabolites below to link to respective articles. [[File ...
Leroux P, Albertini C, Gautier A, Gredt M, Walker AS (July 2007). "Mutations in the CYP51 gene correlated with changes in ... Lanosterol 14α-demethylase (CYP51A1) is a cytochrome P450 enzyme that is involved in the conversion of lanosterol to 4,4- ... Although lanosterol 14α-demethylase is present in a wide variety of organisms, this enzyme is studied primarily in the context ... In particular, this protein catalyzes the removal of the C-14α-methyl group from lanosterol.[2] This demethylation step is ...
SEC14-like protein 2 is a protein that in humans is encoded by the SEC14L2 gene. This gene encodes a cytosolic protein which ... which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol ... "Entrez Gene: SEC14L2 SEC14-like 2 (S. cerevisiae)". Caras IW, Friedlander EJ, Bloch K (1980). "Interactions of supernatant ... Gene. 138 (1-2): 171-4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. Solheim JC, Harris MR, Kindle CS, Hansen TH (1997). " ...
It has also been reported to inhibit mammalian sterol biosynthesis by affecting lanosterol demethylation. Although used in ... "Sterols regulate development and gene expression in Arabidopsis". Plant Physiology. 131 (3): 1258-69. doi:10.1104/pp.014605. ...
Twenty-one resistant genes have been named, mapped, and published. Some cultivars are resistant in one region but susceptible ... now relies on the application of azole fungicides which are demethylase inhibitors that inhibit lanosterol 14 alpha-demethylase ... no evidence of gene flow between Z. pseudotritici and Z. tritici was detected based on sequence analysis of six nuclear loci. ... feature of the Zymoseptoria tritici genome compared to other sequenced plant pathogens was that it contained very few genes for ...
Finally, the enzyme deprotonates to yield lanosterol, which has a hydroxyl group instead of an epoxide. This hydroxyl group can ... "Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes". ... Overview of all the structural information available in the PDB for UniProt: P48449 (Lanosterol synthase) at the PDBe-KB. ... It catalyzes the formation of lanosterol, which is then converted through many steps into cholesterol. The body uses ...
Many of these cholesterol-regulated genes are homologues of fatty acid β-oxidation genes, but have evolved in such a way as to ... Oxidosqualene cyclase then cyclizes squalene to form lanosterol. Finally, lanosterol is converted to cholesterol via either of ... Inherited high cholesterol can also include genetic mutations in the PCSK9 gene and the gene for apolipoprotein B. Elevated ... This is followed by 19 additional steps to convert the resulting lanosterol into cholesterol. A human male weighing 68 kg (150 ...
The structure of the DHCR7 rat gene is very similar to the structure of the human gene. The highest levels of DHCR7 expression ... Through a complicated series of reactions, lanosterol leads to the formation of zymosterol. As shown in a diagram to the right ... The gene encoding DHCR7 (labeled as DHCR7) was cloned in 1998, and has been mapped to chromosome 11q12-13. It is 14100 base ... Genetic models of SLOS are created by knocking out the DHCR7 gene. One study used homologous recombination to disrupt DCHR7 in ...
Consequently, in both the pathogenic fungus Candida albicans and model organism S. cerevisiae mutations in the gene encoding C- ... Kawata S, Trzaskos JM, Gaylor JL (1985). "Microsomal enzymes of cholesterol biosynthesis from lanosterol. Purification and ... Risley John M (2002). "Cholesterol biosynthesis: Lanosterol to cholesterol". Journal of Chemical Education. 79 (3): 377. doi: ... 6-desaturase gene (ERG3) in Candida albicans does not necessarily decrease virulence". Antimicrobial Agents and Chemotherapy. ...
The results show for the first time that in Arabidopsis and all higher plants there are two gene subfamilies: SMT1 and SMT2 ... whereas the same role is played by lanosterol, a tetracyclic triterpene, in non-photosynthetic eukaryotes (fungi, vertebrates ... and has a defect in the gene (STE1) encoding Δ7-sterol-C5(6)-desaturase. Subsequent work allowed the isolation and molecular ...
Here, the isoprene units are joined to make squalene and folded into a set of rings to make lanosterol. Lanosterol can then be ... The expression of the oxidase gene can be upregulated by the steroid sensor PXR when there is a high blood concentration of ... All steroids are manufactured in cells from the sterols lanosterol (opisthokonts) or cycloartenol (plants). Lanosterol and ... which synthesizes the steroid lanosterol. Modifications of lanosterol into other steroids are classified as steroidogenesis ...
Loss-of-function mutations in the gene encoding 7-DHCR result in Smith-Lemli-Opitz syndrome (SLOS) and dramatic accumulation of ... Lanosterol 14α-demethylase (CYP51A1) inhibitors such as clotrimazole, fluconazole, itraconazole, ketoconazole, miconazole, and ... and so lanosterol 14α-demethylase inhibitors are used as antifungals and antiprotozoals in the treatment of infections. ... voriconazole prevent the production of ergosterol from lanosterol. Ergosterol is absent in animals but is an essential ...
V. Lindgren, K. L. Luskey, D. W. Russell, U. Francke: Human genes involved in cholesterol metabolism: chromosomal mapping of ... außerdem wird das Enzym direkt durch Bindung von Cholesterin und besonders Lanosterol, einem anderen Mevalonatderivat gehemmt. ... Bei Cholesterinmangel nimmt die Transkription der Gene und damit die Bildung der HMG-CoA-Reduktase wieder zu. ...
The genomes of 17 plant species contain genes that encode terpenoid synthase enzymes imparting terpenes with their basic ... Squalene is then processed biosynthetically to generate either lanosterol or cycloartenol, the structural precursors to all the ...
The synthesis of sterols such as lanosterol has been observed in G. obscuriglobus. Lanosterol is common in eukaryotes and two ... it is more likely that the existence of eukaryotic traits and genes is explained through lateral gene transfer, and not a more ... Sulfatase genes are also expressed by Planctomycetes as they are metabolically important on the survival of Planctomycetes. The ... The PCR primer which is used often mismatches with the genes creating difficulty when sequencing the genome. When comparing ...
Through several more biosynthetic steps, squalene is transformed into lanosterol, a direct precursor for cholesterol. Notably, ... protein and NF-Y to the farnesyl diphosphate synthase promoter is critical for sterol-regulated expression of the gene". The ...
Genes responsible for the maintenance of cell walls in yeast have been shown to be responsible for angiogenesis in vertebrates ... Azoles (lanosterol 14α-. demethylase inhibitors). Imidazoles. *Topical: bifonazole‡. *butoconazole. *chlormidazole‡. * ...
Craig SP, Buckle VJ, Lamouroux A, Mallet J, Craig I (1986). "Localization of the human tyrosine hydroxylase gene to 11p15: gene ... "Structure of the human tyrosine hydroxylase gene: alternative splicing from a single gene accounts for generation of four mRNA ... "Structure of the human tyrosine hydroxylase gene: alternative splicing from a single gene accounts for generation of four mRNA ... In humans, tyrosine hydroxylase is encoded by the TH gene,[6] and the enzyme is present in the central nervous system (CNS), ...
NCBI gene. 3709. HGNC. structure of the ligand binding suppressor domain of type 1 inositol 1,4,5-trisphosphate receptor 6181 ...
Click on genes, proteins and metabolites below to visit Gene Wiki pages and related Wikipedia articles. The pathway can be ... "ACLY ATP citrate lyase [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2016-11-06.. ...
Click on genes, proteins and metabolites below to link to respective articles. [§ 1] ...
Lees ND, Skaggs B, Kirsch DR, Bard M (March 1995). "Cloning of the late genes in the ergosterol biosynthetic pathway of ... the isoprene units are joined together to make squalene and then folded up and formed into a set of rings to make lanosterol.[ ... LIPID MAPS - Comprehensive lipid and lipid-associated gene/protein databases.. *LipidBank - Japanese database of lipids and ... 95] Lanosterol can then be converted into other steroids such as cholesterol and ergosterol.[95][96] ...
"Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12". J. Bacteriol. 165 (2): 570-8. PMC ... Lanosterol 14 alpha-demethylase. *24-hydroxycholesterol 7α-hydroxylase. 1.14.14: reduced flavin or flavoprotein. *19A1 ...
Spiller, Gene; Margo N. Woods; Sherwood L. Gorbach (27 June 2001). Influence of fiber on the ecology of the intestinal flora. ... stimulate gene expression of glucose transporters in the intestinal mucosa, regulating glucose absorption[98] ... This fermentation influences the expression of many genes within the large intestine,[50] which affect digestive function and ... resulting from upregulation of mineral-transporting genes and their membrane transport proteins within the colon wall. Among ...
The Arabidopsis regulatory gene for production of Anthocyanin Pigment 1 (AtPAP1) can be expressed in other plant species.[42] ... UGT1A6 is a human gene encoding a phenol UDP glucuronosyltransferase active on simple phenols.[107] The enzyme encoded by the ... The phenolic biosynthetic and metabolic pathways and enzymes can be studied by mean of transgenesis of genes. ... The virA gene on the Ti plasmid in the genome of Agrobacterium tumefaciens and Agrobacterium rhizogenes is used by these soil ...
"Efficient Bioconversion of Echinocandin B to Its Nucleus by Overexpression of Deacylase Genes in Different Host Strains" ... Azoles (lanosterol 14α-. demethylase inhibitors). Imidazoles. *Topical: bifonazole‡. *butoconazole. *chlormidazole‡. * ...
stimulate gene expression of glucose transporters in the intestinal mucosa, regulating glucose absorption[112] ... This fermentation influences the expression of many genes within the large intestine,[62] which affect digestive function and ... "Short-chain fatty acids and total parenteral nutrition affect intestinal gene expression". Journal of Parenteral and Enteral ...
"The 2.15 A crystal structure of Mycobacterium tuberculosis chorismate mutase reveals an unexpected gene duplication and ...
A chromosomal microdeletion on Xp11.22 encompassing all of the PHF8 and FAM120C genes and a part of the WNK3 gene was reported ... "Entrez Gene: PHF8 PHD finger protein 8".. *^ Loenarz C, Schofield CJ (Mar 2008). "Expanding chemical biology of 2-oxoglutarate ... PHD finger protein 8 is a protein that in humans is encoded by the PHF8 gene.[5] ... Gene ontology. Molecular function. • oxidoreductase activity, acting on paired donors, with incorporation or reduction of ...
Joanne Wixon; Douglas Kell (2000). „Website Review: The Kyoto Encyclopedia of Genes and Genomes - KEGG". Yeast. 17 (1): 48-55. ...
Lanosterol can then be converted into other sterol such as cholesterol and ergosterol. Organisms vary in their ability to ... Sterck L, Rombauts S, Vandepoele K, Rouzé P, Van de Peer Y (April 2007). "How many genes are there in plants (... and why are ... M. tuberculosis can also grow on the lipid cholesterol as a sole source of carbon, and genes involved in the cholesterol use ... Lees ND, Skaggs B, Kirsch DR, Bard M (March 1995). "Cloning of the late genes in the ergosterol biosynthetic pathway of ...
Through the production of lanosterol may regulate lens protein aggregation and increase transparency. ... 3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. ... "Molecular cloning of the human gene encoding lanosterol synthase from a liver cDNA library.". Baker C.H., Matsuda S.P.T., Liu D ... "Molecular cloning of the human gene encoding lanosterol synthase from a liver cDNA library.". Baker C.H., Matsuda S.P.T., Liu D ...
It transforms lanosterol into 4,4-dimethyl cholesta-8,14,24-triene-3-beta-ol. ... Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. ... Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4- ... Lanosterol 14-alpha demethylase (EC:1.14.14.154*Search proteins in UniProtKB for this EC number. ...
Molecular characterisation of cyp51A and cyp51B genes coding for P450 14alpha-lanosterol demethylases A (CYP51Ap) and B ( ... The cyp51A and cyp51B genes coding for P450 14alpha-sterol demethylases A (CYP51Ap) and B (CYP51Bp) were characterised to ... The cyp51A and cyp51B genes were cloned and the nucleotide sequences were determined. A comparison of the deduced amino acid ...
Analysis of ergosterol and gene expression profiles of sterol Δ5,6-desaturase (ERG3) and lanosterol 14α-demethylase (ERG11) in ...
Gene ID. 4047 (LSS), 16987 (Lss), 81681. Ncbi ID. 9606, NP_002331, NP_001001438.1, NP_001001438, NP_666118.1, NM_031049, NM_ ... Alternative names for Lanosterol synthase antibody. LSS, OSC, Oxidosqualene--lanosterol cyclase, 2,3-epoxysqualene--lanosterol ... Lanosterol synthase (transcript variant 2). Not available. Recombinant protein of human lanosterol synthase (2,3-oxidosqualene- ... Lenti ORF particles, LSS (Myc-DDK-tagged)-Human lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (LSS), transcript ...
... carinii sterol biosynthetic genes encoding the lanosterol 14α demethylase enzyme (Erg11p) (38), the lanosterol synthase enzyme ... Lanosterol production by wild-type yeast (WT) or yeast containing either pYES2.1/ScERG7 (SC) or pYES2.1/PcERG7 (PC). Lanosterol ... Lanosterol synthases are widely regarded as integral membrane proteins (7, 45, 51), and lanosterol synthases from yeast and ... To quantify the amounts of lanosterol directly, gas chromatography was used to measure lanosterol produced by the P. carinii ...
Lanosterol Synthase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Evolution for LSS Gene ENSEMBL:. Gene Tree for LSS (if available). TreeFam:. Gene Tree for LSS (if available). Aminode:. ... Entrez Gene Summary for LSS Gene. * The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to ... GeneCards Summary for LSS Gene LSS (Lanosterol Synthase) is a Protein Coding gene. Diseases associated with LSS include ...
1988) Isolation of the gene for cytochrome P450 L1A1 (lanosterol 14 alpha-demethylase) from Candida albicans. Gene 68:229-237. ... 1987) Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae. DNA Cell Biol. 6:529-537. ... Cloning of CYP51A1 genes from C. albicansclinical isolates.The CYP51A1 genes were cloned from genomic DNA of C. albicans ... Disruption of the S. cerevisiae CYP51A1 gene.The procedure used for the disruption of the S. cerevisiae CYP51A1 gene was taken ...
Lanosterol synthase is encoded by the erg7 gene and catalyzes the cyclization of 2, 3-oxidosqualene, which is a rate-limiting ... Construction of Saccharomyces cerevisiae haploid mutant deficient in lanosterol synthase gene / 药学学报 ... The cassette was transformed into diploid wild strain INVSc1 by LiAc/SS Carrier DNA/PEG method and then erg7 gene haploid ... The results of semiquantitative PCR and real-time quantitative PCR revealed that erg7 expression level in erg7 gene haploid ...
Multiple regulatory elements control expression of the gene encoding the Saccharomyces cerevisiae cytochrome P450, lanosterol ... Expression of the yeast PIS1 gene requires multiple regulatory elements including a Rox1p binding site.. (opens in new tab) ... Transcriptional regulation of the two sterol esterification genes in the yeast Saccharomyces cerevisiae. (opens in new tab) ... Identification of REO1, a gene involved in negative regulation of COX5b and ANB1 in aerobically grown Saccharomyces cerevisiae ...
Lanosterol synthase gene expression was downregulated by antisense RNA technology in S. cerevisiae, which lays a foundation for ... Downregulation of lanosterol synthase gene expression by antisense RNA technology in Saccharomyces cerevisiae / 药学学报 ... Downregulation of lanosterol synthase gene expression by antisense RNA technology in Sacch ... In our study, primers were designed according to erg7 gene sequence of S. cerevisiae. Three fragments including 5 long ...
The aims of this study were to analyse the ergosterol and gene expression profiles of ERG genes in Candida albicans treated ... In addition, ERG3 and ERG11 genes could be probable target of carvacrol against C. albicans. ... Analysis of ergosterol and gene expression profiles of sterol Δ5,6-desaturase (ERG3) and lanosterol 14α-demethylase (ERG11) in ... Analysis of ergosterol and gene expression profiles of sterol Δ5,6-desaturase (ERG3) and lanosterol 14α-demethylase (ERG11) in ...
... lanosterol, to ultimately form ergosterol. The two earliest genes,ERG11 (lanosterol demethylase) andERG24 (C-14 reductase), ... The first three genes;ERG9 (squalene synthase),ERG1 (squalene epoxidase), andERG7 (lanosterol synthase), have been cloned and ... gene andfen1 andfen2 mutations, respectively. The remaining cloned genes,ERG6 (C-24 methylase),ERG2 (D8Æ7 isomerase),ERG3 (C-5 ... Kalb, V.F., Loper, J.C., Dey, C.R., Woods, C.W., and Sutter, T.R. (1986)Gene 45, 237-245.PubMedCrossRefGoogle Scholar ...
In vivo, SREBP-2 preferentially activates genes of cholesterol metabolism, whereas SREBP-1c preferentially activates genes of ... lanosterol 14α-demethylase; G6PD, glucose-6-phosphate dehydrogenase; PGDH, 6-phosphogluconate dehydrogenase; GPAT, glycerol-3- ... Genes regulated by SREBPs. The diagram shows the major metabolic intermediates in the pathways for synthesis of cholesterol, ...
Leroux P, Albertini C, Gautier A, Gredt M, Walker AS (July 2007). "Mutations in the CYP51 gene correlated with changes in ... Lanosterol 14α-demethylase (CYP51A1) is a cytochrome P450 enzyme that is involved in the conversion of lanosterol to 4,4- ... Although lanosterol 14α-demethylase is present in a wide variety of organisms, this enzyme is studied primarily in the context ... In particular, this protein catalyzes the removal of the C-14α-methyl group from lanosterol.[2] This demethylation step is ...
Complete information for FDFT1 gene (Protein Coding), Farnesyl-Diphosphate Farnesyltransferase 1, including: function, proteins ... Pathway: Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3. ... Evolution for FDFT1 Gene ENSEMBL:. Gene Tree for FDFT1 (if available). TreeFam:. Gene Tree for FDFT1 (if available). Aminode:. ... Entrez Gene Summary for FDFT1 Gene. * This gene encodes a membrane-associated enzyme located at a branch point in the ...
In humans, squalene epoxidase is encoded by the SQLE gene. Squalene monooxygenase (SqMO) was formerly referred to as squalene ... 25-epoxylanosterol by lanosterol synthase. Model organisms have been used in the study of SQLE function. A conditional knockout ... "Entrez Gene: SQLE squalene epoxidase". Nagai M, Sakakibara J, Wakui K, Fukushima Y, Igarashi S, Tsuji S, Arakawa M, Ono T (Aug ... Gene. 200 (1-2): 149-56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Lu Y, Dollé ME, Imholz S, van t Slot R, Verschuren ...
The gene ERG11 controls lanosterol creation.[15] Lanosterol is found within the yeast plasma membrane. It is a class of ... This process does not affect all fungi such as Pneumocystis jirovecii, which lacks lanosterol.[18] ... Terconazole binds to the heme iron component on the cytochrome P450 enzyme lanosterol of fungi, also known as CYP3A4. ... Within a normal yeast cell, lanosterol is demethylated using 14α-demethylation.[16] This process creates zymosterol: a major ...
ORI, origin; CHO, cholesterol; LAN, lanosterol; SQA, squalene; MG, monoglycerides; UC, free cholesterol; FFA, free fatty acids ... 4,500 unique human genes (A), or commercial microarrays representing ≈15,000 unique human genes (B). Gene groupings are ... HIV-1 increased gene expression of cholesterol genes in both transformed T-cell lines and primary CD4+ T cells. Consistent with ... HIV-1 Nef induces multiple genes involved in cholesterol biosynthesis and uptake. (A) Gene expression profiles of Jurkat T ...
A lanosterol pathway to plant sterols has been demonstrated in Arabidopsis (Ohyama et al., 2009). The lanosterol pathway only ... Lees, N., Skaggs, B., Kirsch, D., and Bard, M. (1995). Cloning of the late genes in the ergosterol biosynthetic pathway of ... Animals and fungi forms lanosterol catalyzed by lanosterol synthase (LAS) and plants form cycloartenol catalyzed by ... Lanosterol as an alternative precursor for sterols. Plant sterols are synthesized via cycloartenol catalyzed by CAS (Figure 5 ...
Biallelic pathogenic variants in the lanosterol synthase gene LSS involved in the cholesterol biosynthesis cause alopecia with ... Rights & permissionsfor article Biallelic pathogenic variants in the lanosterol synthase gene ,i,LSS,/i, involved in the ...
Lanosterol synthase (LAS) and Cycloartenol synthase (CAS) or ERG7. A. thaliana genome encodes a single CAS1 gene (AT2G07050). ... Thus, 8,7 SI is a single copy gene in A. thaliana. There are two gene copies of 8, 7 SIs in P. trichocarpa with 86% protein ... A gene for this enzyme was proposed based on phylogenomics approach in land plants. The candidate gene was identified as ... A preliminary search has identified three genes to encode ST14R in O. sativa genome. ST14R is a single copy gene in P. ...
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed ... Nucleotide (GenBank) : M23673 Candida tropicalis (ATCC 750) cytochrome P450 lanosterol Nucleotide (GenBank) : AF194419 Candida ... Multiplex real-time PCR targeting the RNase P RNA gene for detection and identification of Candida species in blood. J Clin ... Nucleotide (GenBank) : KU729147 D1/D2 region of 26S rRNA gene Nucleotide (GenBank) : AX110837 Sequence 1570 from Patent ...
We found the expression of genes involved in the cholesterol biosynthesis pathway up-regulated in the daunorubicin-resistant... ... Levels of gene expression for all cholesterol biosynthetic genes in CEM and CEM/R2 were monitored using RT-qPCR (Fig. 1a, b). ... Moreover, since lanosterol has been described as a survival factor [36], we tested the effect of lanosterol on DNR sensitivity ... Expression of gene-encoding proteins involved in cholesterol biosynthesis was normalized to the reference genes RPL13A, RPS18, ...
lanosterol synthase. SNORD159 46194.090. 21q22.3. small nucleolar RNA, C/D box 159. ... List by chromosome of all genes in Atlas. X Y 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 NA - chromosome 21 -. ... These entries belong to a list of genes which are originated of Entrez Genes (NCBI) with a filter on only mapping on hg38 human ... Home GenesLeukemiasSolid TumorsCancer-ProneDeep InsightPortalTeaching. X Y 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 ...
lanosterol increases stability. ISO. RGD:1343716. 6480464. Lanosterol results in increased stability of MARCHF6 protein. CTD. ... Gene Annotator OLGA (Gene List Generator) RatMine GViewer (Genome Viewer) Overgo Probe Designer ACP Haplotyper Genome Scanner ... Gene Ontology Annotations. Biological Process. Term. Qualifier. Evidence. With. Reference. Notes. Source. Original Reference(s) ... Ensembl Genes. ENSMUSG00000039100. Ensembl, ENTREZGENE, UniProtKB/Swiss-Prot. Ensembl Protein. ENSMUSP00000087694. ENTREZGENE, ...
Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4-dimethyl cholesta-8,14,24-triene-3-beta-ol. Gene ... Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. Gene Name:. LSS. ... Gene Name:. DHCR24. Uniprot ID:. Q15392 Molecular weight:. 60100.805. Reactions. Lanosterin + NADPH + Hydrogen Ion → 24,25- ... 1. Lanosterol 14-alpha demethylase. General function:. Involved in monooxygenase activity. Specific function:. ...
Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4-dimethyl cholesta-8,14,24-triene-3-beta-ol. Gene ... Gene Name:. ACY3. Uniprot ID:. Q96HD9 Molecular weight:. Not Available. Reactions. N-Formyl-L-aspartate + Water → Formic acid ... Gene Name:. ASPA. Uniprot ID:. P45381 Molecular weight:. 35734.79. Reactions. N-Formyl-L-aspartate + Water → Formic acid + L- ... Gene Name:. PDF. Uniprot ID:. Q9HBH1 Molecular weight:. 27013.25. Reactions. Formyl-L-methionyl peptide + Water → Formic acid ...
We also analyzed the sequences of 2 genes associated with antifungal resistance: erg11 (lanosterol 14-α demethylase) and fks1 ( ... role of the ERG11 and FKS1 genes in azole and echinocandin resistance. J Antimicrob Chemother. 2018;73:891-9. DOIPubMed ... all isolates had an F126L mutation in the erg11 gene, which is unique to clade III strains and associated with fluconazole ...
The target of azole antifungals is the lanosterol 14-. -demethylase encoded by the ERG11 gene. Several point mutations have ... C-5 sterol desaturase gene) in Candida albicans," Gene, vol. 236, no. 1, pp. 43-51, 1999. View at Publisher · View at Google ... These pumps are encoded by Candida drug resistance 1 and 2 (CDR1 and CDR2) genes in C. albicans [96]. The other pump is a ... Resistance is attributed to point mutations in the FKS1 and/or FKS2 genes (Table 2, [119-121]) which encode the (1,3)-. -D- ...
  • Multiple regulatory elements control expression of the gene encoding the Saccharomyces cerevisiae cytochrome P450, lanosterol 14 alpha-demethylase (ERG11). (meta.org)
  • The major cytochrome P450 in the yeast Saccharomyces cerevisiae, lanosterol 14 alpha-demethylase (ERG11), catalyzes an essential reaction in the biosynthesis of ergosterol, the predominant sterol of yeast. (meta.org)
  • To determine the cis-acting regions responsible for regulation of expression of the ERG11 promoter under optimal conditions of fermentative growth, deletion analysis was performed using the Escherichia coli lacZ as a reporter gene. (meta.org)
  • Sequences resembling ERG11 UAS2 were identified in seven additional oxygen-regulated genes. (meta.org)
  • In addition, ERG3 and ERG11 genes could be probable target of carvacrol against C. albicans. (herbmedpharmacol.com)
  • The two earliest genes, ERG11 (lanosterol demethylase) and ERG24 (C-14 reductase), have been cloned and found to be essential for aerobic growth but are suppressed by mutations in the C-5 desaturase ( ERG3 ) gene and fen1 and fen2 mutations, respectively. (springer.com)
  • The gene ERG11 controls lanosterol creation. (wikipedia.org)
  • Expression of genes involved in development of fluconazole resistance was monitored in each isolate using probes specific for ERG11 (lanosterol 14α-demethylase), MDR1 (a major facilitator), and CDR (ATP-binding cassette or ABC transporter) genes. (asm.org)
  • In this patient, sequence data of the ERG11 gene revealed no point mutations associated with decreased susceptibility. (asm.org)
  • i(5L) leads to increased copy numbers of azole resistance genes present on the left arm of Chr 5, among them TAC1 and ERG11 . (asm.org)
  • Our work shows that azole resistance was due not only to the presence of specific mutations in azole resistance genes (at least ERG11 and TAC1 ) but also to their increase in copy number by LOH and to the addition of extra Chr 5 copies. (asm.org)
  • Azoles, the most commonly used drugs, target the ERG11 gene product, which encodes a lanosterol (C-14) demethylase required for ergosterol biosynthesis ( 30 ). (asm.org)
  • Acquisition of resistance has been shown to be associated with point mutations in the ERG11 gene or in the promoter region of drug transporters ( 17 , 31 ). (asm.org)
  • Several studies have shown that point mutations in the ERG11 gene can result in amino acid alterations that change the protein's affinity for fluconazole, while point mutations in the promoter of CDR1 result in overexpression of the pump. (asm.org)
  • Foram escolhidos os genes da 3-cetoacil-CoA tiolase (ERG10), esqualeno sintase (ERG9 ou SQS1), esqualeno epoxidase (ERG1), oxidoesqualeno-lanosterol ciclase (ERG7) e lanosterol 14-demetilase (ERG11), de L. major e L. braziliensis, além do gene YIP1 de L. braziliensis. (usp.br)
  • Os genes ERG10, SQS1, ERG1, ERG7 e ERG11 de L. major e de L. braziliensis além do YIP1 de L. braziliensis amplificados por PCR foram clonados no vetor pGEM-T Easy, que possibilitou a construção de reagentes para ruptura de todos genes pela inserção da marca HYG e, com exceção do gene ERG7 de L. braziliensis, os genes foram subclonados no vetor pXG1. (usp.br)
  • Com esse objetivo, os genes YIP1, ERG10 e ERG1 de L. braziliensis foram transfectados na linhagem LB2904 de L. braziliensis, e os genes ERG10, SQS1, ERG1, ERG7 e ERG11 e a ruptura do gene ERG1 pelo cassete HYG de L. major foram transfectados na linhagem LT252 de L. major. (usp.br)
  • The P. carinii sterol biosynthetic genes encoding the lanosterol 14α demethylase enzyme (Erg11p) ( 38 ), the lanosterol synthase enzyme (Erg7p) ( 36 ), and the S -adenosyl methionine: C:24 sterol methyltransferase enzyme (Erg6p) ( 21 ) have been isolated, cloned, and expressed in heterologous yeast systems. (pubmedcentralcanada.ca)
  • The cytochrome P-450 lanosterol 14α-demethylase (CYP51A1) of yeasts is involved in an important step in the biosynthesis of ergosterol. (asm.org)
  • The remaining genes not yet cloned are the C-4 demethylase and the C-22 desaturase ( ERG5 ). (springer.com)
  • Lanosterol 14α-demethylase ( CYP51A1 ) is a cytochrome P450 enzyme that is involved in the conversion of lanosterol to 4,4-dimethylcholesta-8(9),14,24-trien-3β-ol. (wikipedia.org)
  • [2] As a member of this family, lanosterol 14α-demethylase is responsible for an essential step in the biosynthesis of sterols . (wikipedia.org)
  • Although lanosterol 14α-demethylase is present in a wide variety of organisms, this enzyme is studied primarily in the context of fungi , where it plays an essential role in mediating membrane permeability. (wikipedia.org)
  • Structure of lanosterol 14α-demethylase (CYP51), as identified by Podust et al. (wikipedia.org)
  • Three-step demethylation of lanosterol, mediated by lanosterol 14α-demethylase. (wikipedia.org)
  • Azoles disrupt the cell membrane by inhibiting the activity of the lanosterol 14- -demethylase [ 21 ], enzyme involved in the biosynthesis of ergosterol (Figure 1 ). (hindawi.com)
  • The largest decreases were seen for squalene epoxidase and lanosterol 14 α demethylase (CYP 51A1). (hindawi.com)
  • Structural and evolutionary studies on sterol 14-demethylase P450 (CYP51), the most conserved P450 monooxygenase: I. Structural analyses of the gene and multiple sizes of mRNA. (wikipathways.org)
  • Nucleotide polymorphisms leading to amino acid substitutions in the lanosterol demethylase gene ( cyp51A ) are associated with reduced susceptibility to azole drugs. (cdc.gov)
  • A common mechanism that confers resistance to azole drugs is a mutation in the lanosterol 14 α-demethylase gene that encodes the CYP51A protein. (cdc.gov)
  • Alterations in the target enzyme (lanosterol 14α-demethylase), including point mutations ( 10 , 11 , 22 , 28 , 32 ) and overexpression ( 31 ), lead to decreased susceptibilities to azole drugs. (asm.org)
  • Genes for lanosterol 14-demethylase, cytochrome P450(14DM), and a mutated inactive cytochrome P450SG1 were cloned from S. cerevisiae strains D587 and SG1, respectively. (nih.gov)
  • 4 ) demonstrated initially that detection of Candida albicans in clinical specimens was possible by PCR by using the lanosterol-demethylase (L1A1) gene as a target for DNA amplification. (asm.org)
  • A Saccharomyces cerevisiae host with an S. cerevisiae genomic library contained in the shuttle vector YEp24 was transformed and the resultant transformants were screened for resistance to ketoconazole (Kc), an inhibitor of the cytochrome P-450 (P-450) enzyme lanosterol 14 alpha-demethylase. (epa.gov)
  • The best-understood avenue for azole resistance involves changes in the cyp51A gene that encodes the target of azole drugs, lanosterol α-14 demethylase. (asm.org)
  • Examples include CYP5A1 , thromboxane A 2 synthase, abbreviated to TBXAS1 ( T hrom B o X ane A 2 S ynthase 1 ), and CYP51A1 , lanosterol 14-α-demethylase, sometimes unofficially abbreviated to LDM according to its substrate ( L anosterol) and activity ( D e M ethylation). (wikipedia.org)
  • Although these organisms were thought to obtain cholesterol by scavenging, transcriptional analyses indicate that Pneumocystis carinii encodes gene homologs involved in sterol biosynthesis. (pubmedcentralcanada.ca)
  • This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. (genecards.org)
  • 1999). The gene mutated in bare patches and striated mice encodes a novel 3beta-hydroxysteroid dehydrogenase. (wikipathways.org)
  • it has also been shown to be involved in weak, heme-dependent activation of ROX1 , which encodes a heme-dependent repressor of the hypoxic genes ( K eng 1992 ). (genetics.org)
  • This gene encodes a member of the NOTCH family of proteins. (cancerindex.org)
  • This gene encodes a member of the cytochrome P450 superfamily of enzymes. (mybiosource.com)
  • This gene encodes an enzyme that catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. (creativebiomart.net)
  • For example, CYP2E1 is the gene that encodes the enzyme CYP2E1 -one of the enzymes involved in paracetamol (acetaminophen) metabolism. (wikipedia.org)
  • Lanosterol synthase is an oxidosqualene cyclase (OSC) enzyme that converts (S)-2,3-oxidosqualene to a protosterol cation and finally to lanosterol. (wikipedia.org)
  • Phylogenetic analysis demonstrates that the E. adriatica eudoraenol synthase is an oxidosqualene cyclase homologous to bacterial lanosterol synthases and distinct from plant triterpenoid synthases. (pnas.org)
  • The present study could open up opportunities not only to carry out functional analysis of a higher-plant-derived oxidosqualene cyclase (OSC) gene in E. coli but also to produce functional triterpenes that originate from medicinal or herbal plants. (deepdyve.com)
  • This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism. (uniprot.org)
  • To better understand the sterol pathway in these uncultivable fungi, yeast deletion strains were used to interrogate the function and localization of P. carinii lanosterol synthase ( ERG7 ). (pubmedcentralcanada.ca)
  • Lanosterol synthase is encoded by the erg7 gene and catalyzes the cyclization of 2, 3-oxidosqualene, which is a rate-limiting step of the inherent mevalonate (MVA)/ ergosterol metabolic pathway in Saccharomyces cerevisiae . (bvsalud.org)
  • Lanosterol synthase gene expression was downregulated by antisense RNA technology in S. cerevisiae, which lays a foundation for reconstructing triterpenoid metabolic pathway in S. cerevisiae by synthetic biology technology . (bvsalud.org)
  • Nine of the eleven genes in the portion of the pathway committed exclusively to ergosterol biosynthesis have been cloned, and their essentiality for aerobic growth has been determined. (springer.com)
  • Terconazole targets fungi specifically since humans do not use lanosterol in this pathway. (wikipedia.org)
  • We found the expression of genes involved in the cholesterol biosynthesis pathway up-regulated in the daunorubicin-resistant leukemia cell line CEM/R2, which is a daughter cell line to the leukemia cell line CCRF-CEM (CEM). (springer.com)
  • Treatment of CEM and CEM/R2 cells with cholesterol biosynthesis inhibitors acting on the enzymes squalene epoxidase and lanosterol synthase, both also involved in the 24,25-epoxycholesterol shunt pathway, revealed a connection of this pathway to lanosterol turnover. (springer.com)
  • As expected, decreases were observed for several genes that encode enzymes of the cholesterol biosynthetic pathway. (hindawi.com)
  • In a different pathway, 24-dehydrocholesterol reductase converts the fat lanosterol into another fat called 24,25-dihydrolanosterol. (medlineplus.gov)
  • Lanosterol synthase (LSS) converts (S)-2,3-epoxysqualene to lanosterol in the cholesterol biosynthesis pathway. (prolekare.cz)
  • Gene ontology analysis revealed that these DEGs are significantly associated with the sterol biosynthetic pathway, and further bioinformatics analysis suggested that sterol regulatory element binding factors (SREBFs) might be key upstream regulators of the DEGs. (frontiersin.org)
  • Unlike the evolutionarily related FXR, which binds bile acids, FXRβ bound lanosterol, an intermediate in the cholesterol synthetic pathway. (sciencemag.org)
  • We introduced into E. coli the biosynthetic pathway genes from farnesyl diphosphate (FPP) to cycloartenol or β-amyrin, which contained Arabidopsis (Arabidopsis thaliana)-derived squalene synthase (AtSQS) and squalene epoxidase (AtSQE) genes in addition to the Arabidopsis cycloartenol synthase (AtCAS1) gene, or the β-amyrin synthase (EtAS) gene of the petroleum plant Euphorbia tirucalli, along with the isopentenyl diphosphate isomerase (HpIDI) gene from a green algae Haematococcus pluvialis. (deepdyve.com)
  • The transcription of the genes of the cholesterol biosynthetic pathway is regulated via the activity of sterol regulatory element-binding proteins (SREBPs), and we found an ∼50% reduction in the amount of the active nuclear form of SREBP in HD cells and mouse brain tissue. (jneurosci.org)
  • showed decreased transcription of the genes encoding for the enzymes of the cholesterol biosynthetic pathway. (jneurosci.org)
  • 6 ] produced the first and second generations of "golden rice", respectively, mainly by expressing exogenous key genes in the β-carotene biosynthesis pathway in the endosperm. (biomedcentral.com)
  • An important gene associated with Candida Glabrata is CSF2 (Colony Stimulating Factor 2), and among its related pathways/superpathways are Th2 Differentiation Pathway and Cytokines and Inflammatory Response . (malacards.org)
  • Through the production of lanosterol may regulate lens protein aggregation and increase transparency. (uniprot.org)
  • This protein is involved in step 3 of the subpathway that synthesizes lanosterol from farnesyl diphosphate. (uniprot.org)
  • The protein encoded by this gene catalyzes the conversion of (S)-2,3 oxidosqualene to lanosterol. (genecards.org)
  • LSS (Lanosterol Synthase) is a Protein Coding gene. (genecards.org)
  • In eukaryotes, lanosterol synthase is an integral monotopic protein associated with the cytosolic side of the endoplasmic reticulum. (wikipedia.org)
  • Lanosterol synthase is a two-domain monomeric protein composed of two connected (α/α) barrel domains and three smaller β-structures. (wikipedia.org)
  • FDFT1 (Farnesyl-Diphosphate Farnesyltransferase 1) is a Protein Coding gene. (genecards.org)
  • In particular, this protein catalyzes the removal of the C-14α- methyl group from lanosterol . (wikipedia.org)
  • Lanosterol is a protein that's found naturally in the lens. (fightforsight.org.uk)
  • Evolutionary analysis of protein and gene structures. (wikipathways.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Thus, as oxygen levels fall to those that limit heme biosynthesis ( 51 ), ROX1 is no longer transcribed ( 84 ), its protein levels fall ( 83 ), and the genes it regulates are derepressed. (asm.org)
  • This work defines the structures of both the murine CCT genes ( Pcyt1a and Pcyt1b ) and identifies a new CCT protein, CCTβ3, with a unique amino terminus that arises from an alternate initiation exon. (chemweb.com)
  • This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. (mybiosource.com)
  • Purified recombinant protein of full-length mouse lanosterol synthase (Lss), with C-terminal MYC/DDK tag, was expressed in HEK293T cells. (creativebiomart.net)
  • In all tissues, sterol regulatory element binding protein (Srebp)-regulated genes involved in cholesterol synthesis showed increased mRNA expression, with the fewest changes detected in the jejunum. (biomedcentral.com)
  • To see if a problem producing lanosterol was involved in causing cataracts somehow, the researchers conducted a number of tests, introducing various types of cataract-like crystalline protein mutations into human lens cells in lab dishes and seeing whether adding lanosterol would clear them away. (baltimoresun.com)
  • The co-expression of a bacterial NADPH-regenerating gene (zwf or gdh) as well as bacterial redox partner protein genes (camA and camB, or NsRED and NsFER) was found to increase the amounts of these triterpenes several fold. (deepdyve.com)
  • Our previous studies identified a positively acting transcription factor called AtrR that binds to the promoter of cyp51A as well as that of an important membrane transporter protein gene called abcG1 . (asm.org)
  • 47, Last updated, Version 1) DE Schizosaccharomyces pombe ribosomal protein L5 gene, complete cds. (bio.net)
  • A strategy consisting of functional expression in Saccharomyces cerevisiae of the C. albicans CYP51A1 genes of sequential clinical isolates from patients was designed. (asm.org)
  • This selection, which was coupled with a test of susceptibility to the azole derivatives fluconazole, ketoconazole, and itraconazole, enabled the detection of mutations in different cloned CYP51A1 genes, whose products are potentially affected in their affinity for azole derivatives. (asm.org)
  • This selection enabled the detection of five different mutations in the cloned CYP51A1 genes which correlated with the occurrence of azole resistance in clinical C. albicans isolates. (asm.org)
  • While the S405F mutation was found as a single amino acid substitution in a CYP51A1 gene from an azole-resistant yeast, other mutations were found simultaneously in individual CYP51A1 genes, i.e. (asm.org)
  • Site-directed mutagenesis of a wild-type CYP51A1 gene was performed to estimate the effect of each of these mutations on resistance to azole derivatives. (asm.org)
  • NCBI/Uniprot data below describe general gene information for CYP51A1 . (mybiosource.com)
  • These genes are likely to be functional based on transcriptional analysis ( 12 ), short-term in vitro inhibition studies ( 19 ), and the incorporation of radiolabeled squalene and mevalonate into P. carinii sterols ( 14 , 20 ). (pubmedcentralcanada.ca)
  • In this study, the transcriptional activation activity of TsNAC1 and RD26 from Arabidopsis was compared with the target genes' promoter regions of TsNAC1 from T. halophila , and the results showed that the transcriptional activation activity of TsNAC1 was higher in tobacco ( Nicotiana tabacum ) and yeast. (plantphysiol.org)
  • In addition to transcriptional repression of cell cycle-related genes, HDACs might also regulate cell cycle progression in a transcription-independent manner. (asm.org)
  • Lanosterol promoted interactions between FXRβ and a nuclear receptor coactivator and stimulated FXRβ-dependent transcriptional activation. (sciencemag.org)
  • Until recently, Rox1 was the only known transcriptional regulator of anaerobically induced genes in S. cerevisiae (reviewed in references 45 , 49 , and 84 ). (asm.org)
  • TAC1 (for transcriptional activator of CDR genes) is critical for the upregulation of the ABC transporters CDR1 and CDR2 , which mediate azole resistance in Candida albicans . (asm.org)
  • These results indicate that insulin has a suppressive effect on the synthesis of the SI complex, presumably by decreasing the transcriptional level of the gene encoding the complex, in small-intestinal epithelial cells. (portlandpress.com)
  • The order of genes, HpIDI, AtSQS, AtSQE, driven by transcriptional read-through from a tac promoter to an rrnB terminator, was crucial for their functional expression in E. coli to produce cycloartenol or β-amyrin. (deepdyve.com)
  • Transcriptional changes included genes required for cell adhesion, actin cytoskeleton regulation, and fatty acid and cholesterol biosynthesis. (jneurosci.org)
  • The expression of PcErg7p in an ERG7 -null mutant of the yeast Saccharomyces cerevisiae did not alter its growth rate and produced a functional lanosterol synthase, as evidenced by the presence of lanosterol detected by gas chromatographic analysis in levels comparable to that produced by the yeast enzyme. (pubmedcentralcanada.ca)
  • The cassette was transformed into diploid wild strain INVSc1 by LiAc/SS Carrier DNA /PEG method and then erg7 gene haploid deficient mutant was obtained by homologous recombination . (bvsalud.org)
  • The results of semiquantitative PCR and real- time quantitative PCR revealed that erg7 expression level in erg7 gene haploid deficient mutant is one time lower than that in wild strain . (bvsalud.org)
  • In our study, primers were designed according to erg7 gene sequence of S. cerevisiae. (bvsalud.org)
  • ERG9 (squalene synthase), ERG1 (squalene epoxidase), and ERG7 (lanosterol synthase), have been cloned and found to be essential for aerobic viability since their absence would result in the cell being unable to synthesize a sterol molecule. (springer.com)
  • Investigators in the field have had to rely on heterologous yeast systems, such as deletion strains of Saccharomyces cerevisiae ( 38 ), or the knockout of genes in the more complicated Schizosaccharomyces pombe system ( 30 ) to assess the function of P. carinii proteins. (pubmedcentralcanada.ca)
  • Cloning and characterization of ERG25, the Saccharomyces cerevisiae gene encoding C-4 sterol methyl oxidase. (wikipathways.org)
  • SACCHAROMYCES cerevisiae is a facultative aerobic organism that possesses two sets of oxygen- and/or heme-regulated genes. (genetics.org)
  • In this study, we used such an approach to obtain a more comprehensive view of the response of Saccharomyces cerevisiae to anaerobiosis and determine the role of Rox1, a key regulator of anaerobic gene expression, in mediating this response. (asm.org)
  • At least seven mutations in the DHCR24 gene have been found to cause desmosterolosis. (medlineplus.gov)
  • Mutations in the 3beta-hydroxysterol Delta24-reductase gene cause desmosterolosis, an autosomal recessive disorder of cholesterol biosynthesis. (medlineplus.gov)
  • At least 18 mutations in the NSDHL gene have been found to cause congenital hemidysplasia with ichthyosiform erythroderma and limb defects (CHILD syndrome). (medlineplus.gov)
  • A few other mutations delete part or all of the NSDHL gene. (medlineplus.gov)
  • Mutations in the NSDHL gene, encoding a 3beta-hydroxysteroid dehydrogenase, cause CHILD syndrome. (medlineplus.gov)
  • However, not all cyp51A mutations contribute to azole resistance (some are benign), and not all azole resistance is caused by mutations in the cyp51A gene ( 12 ). (cdc.gov)
  • Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. (cancerindex.org)
  • Agrawal et al (2011) whole-exome sequencing and gene copy number analyses to study 32 primary hesd and neck squamous cell tumors and found that nearly 40% of 28 mutations identified in NOTCH1 were predicted to truncate the gene product, suggesting that NOTCH1 may function as a tumor suppressor gene rather than an oncogene in HNSCC. (cancerindex.org)
  • Cataractous rats with these 2 gene mutations demonstrated reduced cholesterol levels in the eye lens and cerebral cortex, compared to wild-type rats. (eurekalert.org)
  • First, alterations such as point mutations or upregulation of the gene encoding the target of azoles, an enzyme (Erg11p) involved in ergosterol biosynthesis, can occur. (asm.org)
  • Of the genes differentially expressed in both miconazole- and clobetasol-treated mouse OPCs compared with untreated cells, we identified differentially expressed genes (DEGs) common to both drug treatments. (frontiersin.org)
  • We have identified a set of genes differentially expressed in livers between these two breeds by the mRNA differential display technique (DDRT-PCR) which was first reported by Liang and Pardee (1992) as a fast and efficient method for investigating differences in gene expression. (thefreelibrary.com)
  • Among those differentially expressed genes we identified up-regulation of the TIAF1 (TGF-[beta]1-induced anti-apoptotic factor 1) gene transcripts in Meishan pigs compared with Large White pigs. (thefreelibrary.com)
  • Nearly all organisms respond to changes in their environment by differentially transcribing genes that are important for cell fitness and survival. (asm.org)
  • A two-way mixed models ANOVA was applied to detect differentially expressed genes. (biomedcentral.com)
  • The proportion of false positives, an estimate of the ratio of false positives in the list of differentially expressed genes, was calculated for each tissue. (biomedcentral.com)
  • The number of differentially expressed genes was 420 in the liver, 337 in the duodenum, 402 in the jejunum, and 523 in the ileum. (biomedcentral.com)
  • The list of differentially expressed genes was in turn analyzed by Ingenuity Pathways Analysis (IPA) to detect biological pathways that were overrepresented. (biomedcentral.com)
  • PP242 caused 1,666 genes to be differentially expressed whereas rapamycin affected only 88 genes. (pnas.org)
  • In humans, lanosterol synthase is encoded by the LSS gene. (wikipedia.org)
  • [3] In fungi , CYP51 catalyzes the demethylation of lanosterol to create an important precursor that is eventually converted into ergosterol . (wikipedia.org)
  • Alterations in expression of hepatic genes that could contribute to resistance to dietary cholesterol were investigated in Sprague-Dawley rats, which are known to be resistant to the serum cholesterol raising action of dietary cholesterol. (hindawi.com)
  • Each of these enzymes was able to complement yeast strains containing a deletion of the respective gene, indicating that these P. carinii enzymes likely perform a similar function in P. carinii . (pubmedcentralcanada.ca)
  • Expression of the yeast PIS1 gene requires multiple regulatory elements including a Rox1p binding site. (meta.org)
  • Lanosterol is found within the yeast plasma membrane. (wikipedia.org)
  • Within a normal yeast cell, lanosterol is demethylated using 14α-demethylation. (wikipedia.org)
  • Umate P (2015) Comparison of Genes Encoding Enzymes of Sterol Biosynthesis From Plants to Orthologs in Yeast. (omicsonline.org)
  • The nomenclature GAT1 is used in this paper for the gene encoding the putative major glycerol-3-phosphate acyltransferase of the yeast. (asm.org)
  • S. cerevisiae is one of few yeast species that can grow in the complete absence of oxygen ( 82 ), making it an excellent eukaryotic model for investigating the genomic remodeling of gene expression that is elicited by oxygen deprivation. (asm.org)
  • To determine whether these types of alterations result when DNA repair pathways are eliminated, we constructed yeast strains bearing deletions in six genes involved in mismatch repair ( MSH2 and PMS1 ) or double-strand break repair ( MRE11, RAD50, RAD52 , and YKU80 ). (asm.org)
  • The aims of this study were to analyse the ergosterol and gene expression profiles of ERG genes in Candida albicans treated with carvacrol. (herbmedpharmacol.com)
  • In a series of experiment that went from testing cells in a lab dish, through to dissected rabbit eyes and on to elderly dogs, the team showed that lanosterol treatment stops proteins in the lens from clumping together and makes lenses clearer. (fightforsight.org.uk)
  • Lanosterol, as the substance is known, can reverse the accumulation of proteins in the lens of the eye that appear to cause cataracts, UC San Diego researcher Dr. Kang Zhang and colleagues discovered. (baltimoresun.com)
  • Many genes required for oligodendrocyte differentiation were upregulated in O4 + oligodendrocytes, including numerous genes encoding myelin proteins. (jneurosci.org)
  • Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. (mybiosource.com)
  • Sequencing and analyzing the genomes of the parents and the children, Zhang and his team were able to zero in on a likely cause of the diseased kids' cataracts -- each had two copies of a mutated version of a gene called LSS, which was known to be involved in the production of lanosterol. (baltimoresun.com)
  • The targets used for DNA amplification were the Candida albicans -secreted aspartic proteinase ( SAP ) genes, a multiple-gene family of at least seven members in C. albicans . (asm.org)
  • 5 ) further developed the L1A1-based PCR assay: a nested amplification of the L1A1 gene allowed the direct detection and species-level identification of four species of Candida in clinical specimens. (asm.org)
  • Terconazole binds to the heme iron component on the cytochrome P450 enzyme lanosterol of fungi, also known as CYP3A4. (wikipedia.org)
  • This enzyme is responsible for the conversion of 2,3-oxidosqualene, the last acyclic sterol precursor, into lanosterol, the first sterol intermediate of the mammalian and fungal sterol biosynthetic pathways. (pubmedcentralcanada.ca)
  • Among its related pathways are Regulation of cholesterol biosynthesis by SREBP (SREBF) and cholesterol biosynthesis I . Gene Ontology (GO) annotations related to this gene include intramolecular transferase activity and lanosterol synthase activity . (genecards.org)
  • Because this enzyme catalyzes the formation of precursors far upstream of (S)-2,3-epoxysqualene and cholesterol, statins may negatively influence amounts of intermediates required for other biosynthetic pathways (e.g. synthesis of isoprenoids, coenzyme Q). Thus, lanosterol synthase, which is more closely tied to cholesterol biosynthesis than HMG-CoA reductase, is an attractive drug target. (wikipedia.org)
  • The two pathways producing either lanosterol or cycloartenol are diverged at the step of SQE cyclization (Figure 1) [ 8 ]. (omicsonline.org)
  • In another patient, overexpression of these genes was not detected despite an eightfold increase in fluconazole MIC. (asm.org)
  • Strain A developed resistance over a 2-week period and showed no overexpression of these genes. (asm.org)
  • The study, appearing online on January 26 in advance of print publication in the February 2006 issue of the Journal of Clinical Investigation reports that a primary genetic defect in cataractogenesis is combined mutation of the lanosterol synthase (Lss) and farnesyl diphosphate farnesyl transferase 1 (Fdft1) genes, both of which function in cholesterol biosynthesis. (eurekalert.org)
  • The remaining eight genes encode enzymes which metabolize the first sterol, lanosterol, to ultimately form ergosterol. (springer.com)
  • CTP:phosphocholine cytidylyltransferase (CCT) is a key regulatory enzyme in phosphatidylcholine (PtdCho) biosynthesis, and in mammals, there are two distinct genes that encode enzymes that catalyze this reaction. (chemweb.com)
  • No significant increase was observed in mRNA levels for three other genes involved in cholesterol biosynthesis (PMVK, SCAP, and INSIG2). (asm.org)
  • Since the enzyme affinity for this second substrate is greater than for the monoepoxy (S)-2,3-epoxysqualene, under partial inhibition conversion of 2,3;22,23-diepoxysqualene to 24(S),25-epoxylanosterol is favored over lanosterol synthesis. (wikipedia.org)
  • Cellular 2 H 2 O labelling, mass spectrometry, and isotopomer analysis revealed an increase in lanosterol synthesis which was not accompanied by an increase in cholesterol flux or pool size in CEM/R2 cells. (springer.com)
  • Membrane-bound enzymes of cholesterol synthesis from lanosterol. (wikipathways.org)
  • The NSDHL gene provides instructions for making an enzyme that is involved in the production (synthesis) of cholesterol. (medlineplus.gov)
  • During cholesterol synthesis, the NSDHL enzyme participates in one of several steps that convert a molecule called lanosterol to cholesterol. (medlineplus.gov)
  • Using an Escherichia coli heterologous sterol expression system, we demonstrate that substitution of four amino acid residues in a bacterial lanosterol synthase enabled synthesis of pentacyclic arborinols in addition to tetracyclic sterols. (pnas.org)
  • There are 32 genes in CYP families 5 to 51, most of which are direct orthologs of human CYPs that are involved in endogenous functions including synthesis or inactivation of regulatory molecules. (biomedcentral.com)
  • Due to the enzyme's role in cholesterol biosynthesis, there is interest in lanosterol synthase inhibitors as potential cholesterol-reducing drugs, to complement existing statins. (wikipedia.org)
  • Though some data on the mechanism has been obtained by the use of suicide inhibitors, mutagenesis studies, and homology modeling, it is still not fully understood how the enzyme catalyzes the formation of lanosterol. (wikipedia.org)
  • Interest has grown in lanosterol synthase inhibitors as drugs to lower blood cholesterol and treat atherosclerosis. (wikipedia.org)
  • Researchers looking into families with genetically occurring cataracts, found a mutation in the gene that produces the molecule lanosterol. (mindfood.com)
  • The researchers later found a second family with cataracts that also had a mutation in the LSS gene. (baltimoresun.com)
  • Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. (uniprot.org)
  • Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. (uniprot.org)
  • Lanosterol synthase catalyzes the conversion of (S)-2,3-epoxysqualene to lanosterol, a key four-ringed intermediate in cholesterol biosynthesis. (wikipedia.org)
  • Lanosterol synthase also catalyzes the cyclization of 2,3;22,23-diepoxysqualene to 24(S),25-epoxylanosterol, which is later converted to 24(S),25-epoxycholesterol. (wikipedia.org)
  • The enzyme-catalyzed demethylation of lanosterol is believed to occur in three steps, each of which requires one molecule of diatomic oxygen and one molecule of NADPH (or some other reducing equivalent ). (wikipedia.org)
  • The target sequence of the promoter from the target genes also was identified, and TsNAC1 was shown to target the positive regulators of ion transportation, such as T. HALOPHILA H + -PPASE1 , and the transcription factors MYB HYPOCOTYL ELONGATION-RELATED and HOMEOBOX12 . (plantphysiol.org)
  • Transcriptome evaluation by RNA sequencing (RNA-seq) indicated that both alleles led to elevated transcription of a subset of target genes. (asm.org)
  • Nuclear receptors are transcription factors that, on ligand binding by specific molecules and cofactor recruitment, regulate the expression of specific target genes. (ahajournals.org)
  • Currently, there is no long-term in vitro culture method with which to grow and propagate these fungi, and attempts to functionally characterize genes or to establish effective drug targets have been impeded. (pubmedcentralcanada.ca)
  • This process does not affect all fungi such as Pneumocystis jirovecii, which lacks lanosterol. (wikipedia.org)
  • Host‐induced gene silencing (HIGS) is an approach that shows promise for the control of a variety of problematic crop‐damaging organisms, ranging from nematodes and insects, to fungi and parasitic plants. (els.net)
  • This is especially true for fungi and other eukaryotes that, unlike bacteria, do not participate in active, high-frequency lateral gene transfer. (royalsocietypublishing.org)
  • Treatment of cells with heat-inactivated HIV-1 (2 h at 56°C) did not result in regulation of the cholesterol genes (Fig. 1A , first row), suggesting that intact virus particles are required. (asm.org)
  • Several genes encoding trans -acting factors are known to be involved in this regulation. (genetics.org)
  • The activation of FXRβ by lanosterol may imply that it participates in the regulation of cholesterol biosynthesis in mammalian nonprimates. (sciencemag.org)
  • Functional analyses indicate that a large fraction of the anaerobically induced genes are involved in cell stress (∼1/3), cell wall maintenance (∼1/8), carbohydrate metabolism (∼1/10), and lipid metabolism (∼1/12), with both Rox1 and Upc2 predominating in the regulation of this latter group and Upc2 predominating in cell wall maintenance. (asm.org)
  • Increasing use of zebrafish in drug discovery and mechanistic toxicology demands knowledge of cytochrome P450 (CYP) gene regulation and function. (biomedcentral.com)
  • Various modes of FXR gene regulation. (ahajournals.org)
  • The cyp51A and cyp51B genes coding for P450 14alpha-sterol demethylases A (CYP51Ap) and B (CYP51Bp) were characterised to examine possible drug target modification-dependent resistance to VCZ in this fungus. (nih.gov)
  • T. HALOPHILA STRESS RELATED NAC1 ( TsNAC1 ), cloned from the halophyte Thellungiella halophila , is a NAC transcription factor gene, and its overexpression can improve abiotic stress resistance, especially in salt stress tolerance, in both T. halophila and Arabidopsis ( Arabidopsis thaliana ) and retard the growth of these plants. (plantphysiol.org)
  • Five different patterns of gene expression were observed in isolates recovered from five patients who developed resistance. (asm.org)
  • In general, HIGS utilises ribonucleic acid interference (RNAi) molecules produced by the plant, which then target key genes in pests/pathogens, ideally leading to improved resistance of the plant and a reduction in damage. (els.net)
  • Isolates from these cultures were subjected to antifungal susceptibility testing, DNA karyotyping, and evaluation of the expression of genes previously associated with C. glabrata resistance to fluconazole, CgCDR1 , CgCDR2 , and CgERG11 . (asm.org)
  • Two strains (A and B) of C. glabrata were identified and found to display different patterns of resistance development and gene expression. (asm.org)
  • In contrast, strain B first showed resistance 6 weeks after fluconazole therapy was discontinued but showed overexpression of all three genes. (asm.org)
  • The remaining cloned genes, ERG6 (C-24 methylase), ERG2 (D8Æ7 isomerase), ERG3 (C-5 desaturase), and ERG4 (C-24(28) reductase), have been found to be nonessential. (springer.com)
  • The genes coding for several ABC transporters in C. albicans have been identified, including several CDR genes ( 2 , 16 , 24 , 25 , 30 ). (asm.org)
  • Exogenous addition of lanosterol had a negative effect on CEM/R2 and a positive effect on sensitive CEM cell viability. (springer.com)
  • Additional promoter searches revealed that nearly one-third of the anaerobically induced genes contain an AR1 site(s) for the Upc2 transcription factor, suggesting that Upc2 and Rox1 regulate the majority of anaerobically induced genes in S. cerevisiae . (asm.org)
  • Close to 900 genes were found to have an AtrR response element (ATRE) in their promoter regions. (asm.org)
  • several putative genes involved in sterol biosynthesis were identified through the Pneumocystis Genome Project ( 11 ). (pubmedcentralcanada.ca)
  • Two transcript variants encoding different isoforms have been found for this gene. (mybiosource.com)
  • We confirmed the functional relevance of chalcone synthase , chalcone isomerase , and cytochrome P450 monooxygenase genes using soybean composites and Arabidopsis thaliana mutants, and found that their salt tolerance were positively regulated by chalcone synthase , but was negatively regulated by chalcone isomerase and cytochrome P450 monooxygenase . (mcponline.org)
  • We show that the ATP-competitive inhibitor PP242, but not rapamycin, significantly down-regulates cholesterol biosynthesis genes in a 4E-BP1-dependent manner in NIH 3T3 cells, whereas S6 kinase 1 is the dominant regulator in hepatocellular carcinoma cells. (pnas.org)
  • Specifically, the NSDHL enzyme removes a carbon atom and three hydrogen atoms (a methyl group) in the conversion of lanosterol to cholesterol. (medlineplus.gov)
  • Analysis of shared synteny identified CYP2 gene clusters evolutionarily related to mammalian CYP2 s, as well as unique clusters. (biomedcentral.com)