Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
Computer-based representation of physical systems and phenomena such as chemical processes.
Sequential operating programs and data which instruct the functioning of a digital computer.
A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.
Common name for an extinct species of the Homo genus. Fossils have been found in Europe and Asia. Genetic evidence suggests that limited interbreeding with modern HUMANS (Homo sapiens) took place.
The scientific study of past societies through artifacts, fossils, etc.
Remains, impressions, or traces of animals or plants of past geological times which have been preserved in the earth's crust.
Parliamentary democracy located between France on the northeast and Portugual on the west and bordered by the Atlantic Ocean and the Mediterranean Sea.
Family of the suborder HAPLORHINI (Anthropoidea) comprising bipedal primate MAMMALS. It includes modern man (HOMO SAPIENS) and the great apes: gorillas (GORILLA GORILLA), chimpanzees (PAN PANISCUS and PAN TROGLODYTES), and orangutans (PONGO PYGMAEUS).
The study of early forms of life through fossil remains.
The period of history before 500 of the common era.
Affection; in psychiatry commonly refers to pleasure, particularly as it applies to gratifying experiences between individuals.
The performance of the basic activities of self care, such as dressing, ambulation, or eating.
The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Diseases of the domestic cat (Felis catus or F. domesticus). This term does not include diseases of the so-called big cats such as CHEETAHS; LIONS; tigers, cougars, panthers, leopards, and other Felidae for which the heading CARNIVORA is used.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.
Application of statistical procedures to analyze specific observed or assumed facts from a particular study.
A naphthacene antibiotic that inhibits AMINO ACYL TRNA binding during protein synthesis.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A synthetic tetracycline derivative with similar antimicrobial activity.
Potent cholinesterase inhibitor used as an insecticide and acaricide.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
A zinc-containing sialoglycoprotein that is used to study aminopeptidase activity in the pathogenesis of hypertension. EC
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
A diverse genus of minute freshwater CRUSTACEA, of the suborder CLADOCERA. They are a major food source for both young and adult freshwater fish.
The study of ENVIRONMENTAL POLLUTION and the toxic effects of ENVIRONMENTAL POLLUTANTS on the ECOSYSTEM. The term was coined by Truhaut in 1969.
A suborder of CRUSTACEA, order Diplostraca, comprising the water fleas. They are benthic filter feeders that consume PHYTOPLANKTON. The body is laterally compressed and enclosed in a bivalved carapace, from which the head extends.
Any of several Old World finches of the genus Serinus.
The science concerned with the detection, chemical composition, and biological action of toxic substances or poisons and the treatment and prevention of toxic manifestations.
A plant genus of the family Passifloraceae, order Violales, subclass Dilleniidae, class Magnoliopsida. They are vines with ornamental flowers and edible fruit.
The formation of dentin. Dentin first appears in the layer between the ameloblasts and odontoblasts and becomes calcified immediately. Formation progresses from the tip of the papilla over its slope to form a calcified cap becoming thicker by the apposition of new layers pulpward. A layer of uncalcified dentin intervenes between the calcified tissue and the odontoblast and its processes. (From Jablonski, Dictionary of Dentistry, 1992)
A love or pursuit of wisdom. A search for the underlying causes and principles of reality. (Webster, 3d ed)
Activities concerned with governmental policies, functions, etc.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
A plant genus of the family LINACEAE that is cultivated for its fiber (manufactured into linen cloth). It contains a trypsin inhibitor and the seed is the source of LINSEED OIL.
A group of fatty acids, often of marine origin, which have the first unsaturated bond in the third position from the omega carbon. These fatty acids are believed to reduce serum triglycerides, prevent insulin resistance, improve lipid profile, prolong bleeding times, reduce platelet counts, and decrease platelet adhesiveness.
Regular course of eating and drinking adopted by a person or animal.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
A food group comprised of EDIBLE PLANTS or their parts.

Separation of shoot and floral identity in Arabidopsis. (1/56413)

The overall morphology of an Arabidopsis plant depends on the behaviour of its meristems. Meristems derived from the shoot apex can develop into either shoots or flowers. The distinction between these alternative fates requires separation between the function of floral meristem identity genes and the function of an antagonistic group of genes, which includes TERMINAL FLOWER 1. We show that the activities of these genes are restricted to separate domains of the shoot apex by different mechanisms. Meristem identity genes, such as LEAFY, APETALA 1 and CAULIFLOWER, prevent TERMINAL FLOWER 1 transcription in floral meristems on the apex periphery. TERMINAL FLOWER 1, in turn, can inhibit the activity of meristem identity genes at the centre of the shoot apex in two ways; first by delaying their upregulation, and second, by preventing the meristem from responding to LEAFY or APETALA 1. We suggest that the wild-type pattern of TERMINAL FLOWER 1 and floral meristem identity gene expression depends on the relative timing of their upregulation.  (+info)

Characterization of an amphioxus paired box gene, AmphiPax2/5/8: developmental expression patterns in optic support cells, nephridium, thyroid-like structures and pharyngeal gill slits, but not in the midbrain-hindbrain boundary region. (2/56413)

On the basis of developmental gene expression, the vertebrate central nervous system comprises: a forebrain plus anterior midbrain, a midbrain-hindbrain boundary region (MHB) having organizer properties, and a rhombospinal domain. The vertebrate MHB is characterized by position, by organizer properties and by being the early site of action of Wnt1 and engrailed genes, and of genes of the Pax2/5/8 subfamily. Wada and others (Wada, H., Saiga, H., Satoh, N. and Holland, P. W. H. (1998) Development 125, 1113-1122) suggested that ascidian tunicates have a vertebrate-like MHB on the basis of ascidian Pax258 expression there. In another invertebrate chordate, amphioxus, comparable gene expression evidence for a vertebrate-like MHB is lacking. We, therefore, isolated and characterized AmphiPax2/5/8, the sole member of this subfamily in amphioxus. AmphiPax2/5/8 is initially expressed well back in the rhombospinal domain and not where a MHB would be expected. In contrast, most of the other expression domains of AmphiPax2/5/8 correspond to expression domains of vertebrate Pax2, Pax5 and Pax8 in structures that are probably homologous - support cells of the eye, nephridium, thyroid-like structures and pharyngeal gill slits; although AmphiPax2/5/8 is not transcribed in any structures that could be interpreted as homologues of vertebrate otic placodes or otic vesicles. In sum, the developmental expression of AmphiPax2/5/8 indicates that the amphioxus central nervous system lacks a MHB resembling the vertebrate isthmic region. Additional gene expression data for the developing ascidian and amphioxus nervous systems would help determine whether a MHB is a basal chordate character secondarily lost in amphioxus. The alternative is that the MHB is a vertebrate innovation.  (+info)

Bone resorption induced by parathyroid hormone is strikingly diminished in collagenase-resistant mutant mice. (3/56413)

Parathyroid hormone (PTH) stimulates bone resorption by acting directly on osteoblasts/stromal cells and then indirectly to increase differentiation and function of osteoclasts. PTH acting on osteoblasts/stromal cells increases collagenase gene transcription and synthesis. To assess the role of collagenase in the bone resorptive actions of PTH, we used mice homozygous (r/r) for a targeted mutation (r) in Col1a1 that are resistant to collagenase cleavage of type I collagen. Human PTH(1-34) was injected subcutaneously over the hemicalvariae in wild-type (+/+) or r/r mice four times daily for three days. Osteoclast numbers, the size of the bone marrow spaces and periosteal proliferation were increased in calvariae from PTH-treated +/+ mice, whereas in r/r mice, PTH-induced bone resorption responses were minimal. The r/r mice were not resistant to other skeletal effects of PTH because abundant interstitial collagenase mRNA was detected in the calvarial periosteum of PTH-treated, but not vehicle-treated, r/r and +/+ mice. Calcemic responses, 0.5-10 hours after intraperitoneal injection of PTH, were blunted in r/r mice versus +/+ mice. Thus, collagenase cleavage of type I collagen is necessary for PTH induction of osteoclastic bone resorption.  (+info)

Molecular cloning and epitope analysis of the peanut allergen Ara h 3. (4/56413)

Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The recombinant form of this protein was expressed in a bacterial system and was recognized by serum IgE from approximately 45% of our peanut-allergic patient population. Serum IgE from these patients and overlapping, synthetic peptides were used to map the linear, IgE-binding epitopes of Ara h 3. Four epitopes, between 10 and 15 amino acids in length, were found within the primary sequence, with no obvious sequence motif shared by the peptides. One epitope is recognized by all Ara h 3-allergic patients. Mutational analysis of the epitopes revealed that single amino acid changes within these peptides could lead to a reduction or loss of IgE binding. By determining which amino acids are critical for IgE binding, it might be possible to alter the Ara h 3 cDNA to encode a protein with a reduced IgE-binding capacity. These results will enable the design of improved diagnostic and therapeutic approaches for food-hypersensitivity reactions.  (+info)

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (5/56413)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Cytochrome P450 monooxygenases and insecticide resistance in insects. (6/56413)

Cytochrome P450 monooxygenases are involved in many cases of resistance of insects to insecticides. Resistance has long been associated with an increase in monooxygenase activities and with an increase in cytochrome P450 content. However, this increase does not always account for all of the resistance. In Drosophila melanogaster, we have shown that the overproduction of cytochrome P450 can be lost by the fly without a corresponding complete loss of resistance. These results prompted the sequencing of a cytochrome P450 candidate for resistance in resistant and susceptible flies. Several mutations leading to amino-acid substitutions have been detected in the P450 gene CYP6A2 of a resistant strain. The location of these mutations in a model of the 3D structure of the CYP6A2 protein suggested that some of them may be important for enzyme activity of this molecule. This has been verified by heterologous expression of wild-type and mutated cDNA in Escherichia coli. When other resistance mechanisms are considered, relatively few genetic mutations are involved in insecticide resistance, and this has led to an optimistic view of the management of resistance. Our observations compel us to survey in more detail the genetic diversity of cytochrome P450 genes and alleles involved in resistance.  (+info)

Leptin suppression of insulin secretion and gene expression in human pancreatic islets: implications for the development of adipogenic diabetes mellitus. (7/56413)

Previously we demonstrated the expression of the long form of the leptin receptor in rodent pancreatic beta-cells and an inhibition of insulin secretion by leptin via activation of ATP-sensitive potassium channels. Here we examine pancreatic islets isolated from pancreata of human donors for their responses to leptin. The presence of leptin receptors on islet beta-cells was demonstrated by double fluorescence confocal microscopy after binding of a fluorescent derivative of human leptin (Cy3-leptin). Leptin (6.25 nM) suppressed insulin secretion of normal islets by 20% at 5.6 mM glucose. Intracellular calcium responses to 16.7 mM glucose were rapidly reduced by leptin. Proinsulin messenger ribonucleic acid expression in islets was inhibited by leptin at 11.1 mM, but not at 5.6 mM glucose. Leptin also reduced proinsulin messenger ribonucleic acid levels that were increased in islets by treatment with 10 nM glucagon-like peptide-1 in the presence of either 5.6 or 11.1 mM glucose. These findings demonstrate direct suppressive effects of leptin on insulin-producing beta-cells in human islets at the levels of both stimulus-secretion coupling and gene expression. The findings also further indicate the existence of an adipoinsular axis in humans in which insulin stimulates leptin production in adipocytes and leptin inhibits the production of insulin in beta-cells. We suggest that dysregulation of the adipoinsular axis in obese individuals due to defective leptin reception by beta-cells may result in chronic hyperinsulinemia and may contribute to the pathogenesis of adipogenic diabetes.  (+info)

Alternative sulfonylurea receptor expression defines metabolic sensitivity of K-ATP channels in dopaminergic midbrain neurons. (8/56413)

ATP-sensitive potassium (K-ATP) channels couple the metabolic state to cellular excitability in various tissues. Several isoforms of the K-ATP channel subunits, the sulfonylurea receptor (SUR) and inwardly rectifying K channel (Kir6.X), have been cloned, but the molecular composition and functional diversity of native neuronal K-ATP channels remain unresolved. We combined functional analysis of K-ATP channels with expression profiling of K-ATP subunits at the level of single substantia nigra (SN) neurons in mouse brain slices using an RT-multiplex PCR protocol. In contrast to GABAergic neurons, single dopaminergic SN neurons displayed alternative co-expression of either SUR1, SUR2B or both SUR isoforms with Kir6.2. Dopaminergic SN neurons expressed alternative K-ATP channel species distinguished by significant differences in sulfonylurea affinity and metabolic sensitivity. In single dopaminergic SN neurons, co-expression of SUR1 + Kir6.2, but not of SUR2B + Kir6.2, correlated with functional K-ATP channels highly sensitive to metabolic inhibition. In contrast to wild-type, surviving dopaminergic SN neurons of homozygous weaver mouse exclusively expressed SUR1 + Kir6.2 during the active period of dopaminergic neurodegeneration. Therefore, alternative expression of K-ATP channel subunits defines the differential response to metabolic stress and constitutes a novel candidate mechanism for the differential vulnerability of dopaminergic neurons in response to respiratory chain dysfunction in Parkinson's disease.  (+info)

Abstract: Interleukin-2 (IL-2) gene regulation was investigated in primary cultures of highly purified human peripheral blood CD28+T cells. Two discrete mechanisms for induction of T-cell proliferation could be distinguished by examining cell cycle progression and the expression of the IL-2 gene. Stimulation of cells by CD3 MoAb induced only transiently expressed, small amounts of IL-2 mRNA that was completely suppressed by cyclosporine. Costimulation of T cells with CD3 MoAb and either CD28 MoAb or PMA, but not calcium ionophore, induced a 50-100-fold increased in IL-2 gene expression and secretion. High levels of IL-2 gene expression could also be achieved by stimulation with calcium ionophore and PMA or CD28 MoAb and PMA, but not by CD28 MoAb plus calcium ionophore. IL-2 gene expression and T-cell proliferation induced by CD3 MoAb plus PMA or calcium ionophore plus PMA were completely suppressible by cyclosporine. In contrast, IL-2 gene expression and T-cell proliferation induced by CD28 MoAb ...
Read SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology, Genome Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Regulation of transforming growth factor β1 gene expression by the product of the retinoblastoma-susceptibility gene Academic Article ...
DNA actively 58-49-1 biological activity down-regulates the gene expression of LL37 in monocytes and epithelial cells. Nevertheless, due to the fact
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மரபணு வெளிப்பாடு (Gene expression) என்பது மரபணுவில் இருக்கும் தகவல்கள், தொழிற்படக்கூடிய மரபணு உற்பத்திப்பொருளாக (gene product) மாற்றப்படும் செயல்முறையாகும். மரபணுவிலிருக்கும் மரபணுக் குறியீட்டுப்பகுதியில் (coding region) இருந்து இவ்வகையான மரபணு உற்பத்திப்பொருட்கள் உருவாகின்றன. மரபணு உற்பத்திப்பொருட்கள் உயிர்வேதியியல் பொருட்களாகும். பொதுவாக இந்த மரபணு உற்பத்திப் பொருட்கள் தொழிற்படும் ...
Effect of in vivo gene expression knockdown on neuroblast migration in the olfactory bulb. The RMS within the olfactory bulb (bRMS) is visible as more intensely
What is meant by gene expression? Gene expression refers to genes being turned on and producing a product. The product could be an enzyme, a structural protein, or a control molecule. Studies of gene expression typically measure the production of mRNA. Most mechanisms that control gene expression do so by controlling transcription, the synthesis of mRNA. However there are other mechanisms for controlling the rate of protein synthesis that occur downstream (between transcription and translation). Several of these are described in this animation. View the animation below, then complete the quiz to test your knowledge of the concept.. ...
Determining gene expression on a single pair of microarrays. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Genomes contain all the genetic information needed by an organism, regardless of the circumstance or environment. Generally, however, even single-cell organisms use only a fraction of their genome at any time; that is, cells generally express only a fraction of their genome at any given time. This is especially true among the individual cells that make up multicellular organisms, where cell specialization (differentiation) dictates that only certain genes be expressed in certain cells, and only at particular times during the organisms life span.. ...
Process of gene expression entails plasmid DNA or mRNA delivery to specific cells and is a technique routinely utilized in labs to study a gene of interest or a signaling pathway. Gene expression usually allows transient protein production, but in some cases stable integration into a genome and continuous protein production over time is required.
I want to show relationship between down regulation and up regulation of expression of a gene with that of patients relapse status in cancer. However, I dont have enough data to plot KM/ Survival curve. All I have is disease status and gene expression after 1 year and 3 years. I was thinking of making Box whisker plot but I have not seen box whisker showing correlation between gene expression and survival. Any suggestions how best I can show such relationship?. ...
Gene expression is the process by which genetic information is used to produce proteins, essential for cells to function properly. It takes place in two steps (first the transcription, then the translation), considered to be two independent processes until now. Today, microbiologists provide additional evidence that they are intrinsically related and that a protein complex called Ccr4-Not plays a key role in gene expression by acting as a messenger between the nucleus and the cytoplasm.
Gene Expression Macro Version 1.1 Instructions Rev B 1 Bio-Rad Gene Expression Macro Users Guide 2004 Bio-Rad Laboratories Table of Contents: Introduction Opening
Hi All,. I want examine different gene expression for different treatment by using qRT-PCR , i will not do any sequencing or assembly . First RNA will be extract and then my question is How can i choose the candidate genes before run PCR ??? Then i can design the primer for the candidate and run PCR.. I hope if someone can explain and suggest methods for chose the candidate ... Thanks. ...
Is a useful technique for acquiring the gene expression pattern of a number of selected genes due to its high sensitivity, specificity and broad quantification
This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E
The answer, though, is differences in gene expression in a cell. Clones have identical DNA, but the way the DNA is transcribed to RNA, and the way the RNA is translated to making a protein, can be specific to the cell. There are epigenetic factors - basically, chemicals that adhere to certain parts of the genome - that dictate NOT whether the DNA is there, but how often the DNA is read to make RNA and how efficiently that is made into functional proteins. Think about a fertilized egg. It divides into two cells, then 4 cells, then 8 cells, then 16 cells, then 32 cells, etc. Every time the cell divides, it has to make a perfect copy of its DNA. The cell machinery isnt always perfect and little changes in DNA can occur. Also, those chemicals that can affect gene expression can get added or subtracted as cells divide. Many of them, however, are passed on to the daughter cells. Thats how identical cells can have different levels of gene expression, and how people and horses and dogs with identical ...
Akhilesh Bajpai, Sravanthi Davuluri and Acharya KK* (*Correspondence: [email protected]), A comparative account of gene expression resources, feature-validations and application-based categorizations; In: Startbioinfo; 12 Oct 2011, ...
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An expression system refers to the factors that work together to yield a particular gene product such as a protein, ribozyme or RNA particle.
Effect of mannan-oligosaccharides (MOS) supplementation onperformance, immunity and HSP70 gene expression in broilerchicken during hot-dry summer
I am fascinated by a fundamental question in biology; How is regulation of gene expression as a response to external stimulus achieved? My laboratory
Chapter 18 Regulation of Gene Expression Gene Regulation Is Necessary By switching genes off when they are not needed, cells can prevent resources from being wasted. There should be natural selection
EPFL chemists have uncovered the first steps in the process of gene expression by showing how the protein Rap1 pries open the tightly wound, compacted structure of DNA in the cell to gain access to specific genes.
Im just not quite sure the answer to this. When we talk about epigenetics and gene expression, does it not matter if we have snps or not? For...
A new study presents the first analysis of the pilot dataset from the Genotype-Tissue Expression (GTEx) project, which investigates how our underlying DNA regulates gene expression.
Genotyping & gene expression full services from Eurofins Genomics . Wide range of platforms and assays available ** Call +49 8092 8289-0
Telencephalic and diencephalic gene expression in the fgf16 morphants.The expression of emx1 (A, B), tbr1 (C, D), dlx2 (E, F), pax6a (G, H), and shh (I, J) in w
This function will fit smooth spline curves for the gene expression dynamics along pseudotime in a gene-wise manner and return the corresponding response matrix. This function is build on other functions (fit_models and responseMatrix) and used in calILRs and calABCs functions
You need to make cDNA with random hexamers or oligo dT, then amplify the gene with specific primers after that by PCR. In my experience there isnt a whole lot of difference between random hexamers or oligo dT. They seem to work equally as well for cDNA generation.. Lately, I have been using the Invitrogen SuperScript III kit with random hexamers to amplify my cDNAs. Works great.. Rb. ...
We have previously shown that the intramuscular injection of naked plasmid DNA enables foreign gene expression in muscle. Further studies showed that the intravascular delivery of naked plasmid DNA enables high levels of expression not only in muscle but also in hepatocytes. For the liver, this tech …
BACKGROUND: Although, systematic analysis of gene annotation is a powerful tool for interpreting gene expression data, it sometimes is blurred by incomplete gene annotation, missing expression response of key genes and secondary gene expression responses. These shortcomings may be partially circumvented by instead matching gene expression signatures to signatures of other experiments. FINDINGS: To facilitate this we present the Functional Association Response by Overlap (FARO) server, that match input signatures to a compendium of 242 gene expression signatures, extracted from more than 1700 Arabidopsis microarray experiments. CONCLUSIONS: Hereby we present a publicly available tool for robust characterization of Arabidopsis gene expression experiments which can point to similar experimental factors in other experiments. The server is available at ...
CG000170_TechNote_BiologicalandTechnicalVariationinSingleCell3GeneExpressionExperiments_RevA_.pdf. Technical Note - Biological & Technical Variation in Single Cell Gene Expression Experiments. The Chromium Single Cell 3′ v2 Reagent Kits protocol (Document CG00052) produces Single Cell 3′ short-read sequencer compatible libraries. Technical and biological variation may be present in the experiment design, and may impact data interpretation. Potential sources of technical variation include running a sample on two separate microfluidic chips or at different well positions on the same chip, and or technical variation introduced by sequencing libraries on separate Illumina flowcells or sequencing lanes. This Technical Note examines the potential sources of technical and biological variation and their effects on single cell gene expression. These factors need to be considered when designing an experiment to minimize bias and generate reliable single cell gene expression data.. FOR USE WITH. ...
Researchers use DNA microarrays, or gene chips, to distinguish among different types of tissues based on the expression patterns of thousands of genes. A new study says the technology can be modified to profile more tissue samples simultaneously and with greater efficiency. The innovation is called an array of arrays. Gene expression profiles are typically obtained one at a time by hybridizing a single tissue sample to a single array on an individual glass slide. The integrated device described in the study is a glass wafer that includes 49 individual oligonucleotide arrays arranged as a 7 × 7 array of arrays. David J. Lockhart and colleagues at the Genomics Institute of the Novartis Research Foundation, in San Diego, California, developed a way to hybridize many tissue samples to multiple arrays on a single glass slide, or wafer. Using this and other modifications, they completed a study of gene expression in ovarian cancer in a single experiment. This was done in a fraction of the time and ...
Background Despite sharing the same genes, identical twins demonstrate substantial variability in behavioral traits and in their risk for disease. Epigenetic factors-DNA and chromatin modifications that affect levels of gene expression without affecting the DNA sequence-are thought to be important in establishing this variability. Epigenetically-mediated differences in the levels of gene expression that are associated with individual variability traditionally are thought to occur only in a gene-specific manner. We challenge this idea by exploring the large-scale organizational patterns of gene expression in an epigenetic model of behavioral variability. Methodology/Findings To study the effects of epigenetic influences on behavioral variability, we examine gene expression in genetically identical mice. Using a novel approach to microarray analysis, we show that variability in the large-scale organization of gene expression levels, rather than differences in the expression levels of specific genes, is
Quantitative gene expression analysis aims to define the gene expression patterns determining cell behavior. So far, these assessments can only be performed at the population level. Therefore, they determine the average gene expression within a population, overlooking possible cell-to-cell heterogeneity that could lead to different cell behaviors/cell fates. Understanding individual cell behavior requires multiple gene expression analyses of single cells, and may be fundamental for the understanding of all types of biological events and/or differentiation processes. We here describe a new reverse transcription-polymerase chain reaction (RT-PCR) approach allowing the simultaneous quantification of the expression of 20 genes in the same single cell. This method has broad application, in different species and any type of gene combination. RT efficiency is evaluated. Uniform and maximized amplification conditions for all genes are provided. Abundance relationships are maintained, allowing the ...
Gene Expression Analysis Market-[By Technology (DNA Microarray, Real-Time PCR, Next Generation Sequencing), By Consumables (DNA chips, Reagents), By Services (Gene Profiling, Bioinformatics, Data Analysis Software) & Applications]-Global Forecast to 2018. The global gene expression analysis market is witnessing a significant growth and will continue to do so in the next five years. The factors contributing to this growth are increased funding scenario worldwide, increased government involvement, developments in research for diseases like cancer, and the use of gene expression in drug discovery and personalized medicine. The Asian region is projected to have the highest growth rate with growth hinged at China, India, and Japan. Apart from Asia, countries such as Turkey, Brazil, and South Africa too have a high projected growth.. In the Asian region, China is the genomic leader. China has cultivated a favorable condition to support gene expression analysis growth. From its humble beginning in 1999 ...
The most comprehensive gene expression qPCR assay selection in the industry- helping you achieve highly sensitive and specific detection from your research.. TaqMan Gene Expression Assays are probe and primer sets based on 5 nuclease chemistry using TaqMan MGB (minor groove binder) probes, the most quantitative gene expression technology available.. ...
Gene Expression Analysis can provide an ideal blend of high-level analysis and granular detail when studying a biological system. We know that the RNA levels of an organisms genes not only determine the systems overall status, but also the functioning of the many molecular pathways in the organism. We also know that activity in these pathways can make or break a commercial product or production process. Lets see how Time-Series Gene Expression Analysis can address these challenges.. The key is to is to look at the dynamics in gene expression, this means studying the ways in which gene expression changes during a biological process. As the price of RNA-seq data has come down, it has become very feasible to analyze time series RNA-seq data from organisms in commercial biological products and industrial production processes. The focus then is on deploying tools and expertise to use the information contained in the tens of thousands of data points that these time series experiments produce, to ...
It is gene expression that defines a cells identity. Science, Sciences Science of Aging Knowledge Environment (SAGE KE), and Sciences STKE focus attention on the highly dynamic nature of gene expression. Mechanisms governing selective gene expression in multigene families, as well as those by which cis- and trans-acting factors contribute to regulation of gene expression, are topics under discussion. In addition, new methodologies allow researchers to gain insight into gene expression at the level of single cells and provide a glimpse at the real-time interactions among proteins and DNA.. ...
Inflammation involves timed gene expression, suggesting that the fine-tuned onset, amplitude, and termination of expression of hundreds of genes is of critical importance to organismal homeostasis. Recent study of post-transcriptional regulation of inflammatory gene expression led to the suggestion of a regulatory role for pre-mRNA splicing. Here, using a hybrid capture approach to purify incompletely spliced, chromatin-associated pre-mRNAs, we use deep sequencing to study pre-mRNA splicing of the NF-kB transcriptome. By freezing transcription and examining subsequent splicing of complete transcripts, we find many introns splice tens to hundreds of times slower than average. In many cases, this is attributable to poor splice donor sequences that are evolutionarily conserved. When these introns were altered by ~2 base pairs to yield stronger splice donors, gene expression levels increased markedly for several genes in the context of a reporter system. We propose that such splice sites represent a ...
The Gene Expression Metasignatures (GEMS) analysis tool was developed to allow researchers to determine a weighted consensus across multiple datasets for the regulation of a gene by acute (0-4 h) or chronic (24 h) treatment with 17beta-estradiol in MCF-7 cells. GEMS was retired in November 2012. In its place we have developed a broader, large-scale differential gene expression tool for the nuclear receptor signaling research community, Transcriptomine. Transcriptomine collates omics-scale differential gene expression studies across the nuclear receptor and coregulator signaling field into a single analysis tool, allowing researchers to detect patterns across multiple datasets. A large number of 17beta-estradiol MCF-7 datasets have been uploaded to the database, including all those originally in GEMS. These can currently be viewed as individual fold changes.. ...
The primary goal of the study is to measure in the intact human heart, the alterations in gene expression over time that are associated with reverse remodeling in response to β-blockade. The second goal is to investigate the signaling mechanisms which in turn are responsible for these changes in gene expression, and the third goal is to determine the relationship between intrinsic systolic dysfunction and remodeling of the left ventricle. This will be accomplished by measuring ventricular size, function, and gene expression in myocardial tissue samples obtained by percutaneous biopsy prior to initiation of β-blockade and at 3 and 12 months after start of therapy. The specific Aims and Hypotheses to be tested are:. ...
The edge package implements methods for carrying out differential expression analyses of genome-wide gene expression studies. Significance testing using the optimal discovery procedure and generalized likelihood ratio tests (equivalent to F-tests and t-tests) are implemented for general study designs. Special functions are available to facilitate the analysis of common study designs, including time course experiments. Other packages such as snm, sva, and qvalue are integrated in edge to provide a wide range of tools for gene expression analysis.
Relative gene expression analysis requires precise, powerful and cutting edge technology called Real Time PCR, that is being utilized today by many research laboratories around the world.
Weight Loss, Gene Expression and Excretion. Losing weight is NOT about counting calories because not all calories (foods we eat) are created equally. A calorie consumed is different than a calorie of energy that you burn off through exercise because of a concept called gene expression. The foods you eat affect your bodys ability to alter gene expression. There are two basic types of genes: constitutional and inducible. Constitutional genes encode DNA and RNA messages that are expressed in a constant fashion and are not generally modified by environmental or lifestyle choices. Inducible genes, however, are sensitive to toxins, nutritional influences, and the environment and can be up-regulated or down regulated in their expression. These inducible genes play a large role in the aging process and in the livers ability handle detoxification and excretion.. Our genetic inheritance also plays a large role in how vulnerable we are to age-related diseases via the inducible gene system. A good ...
Seker, H. (2004) A Multi-Fuzzy Filtering Approach to Reliable Gene Expression Profile Analysis. Proceedings of the 2004 IEEE Symposium on Computational Intelligence in Bioinformatics and Computational Biology, October 2004, pp. 37-40 ...
View Notes - Gene Expression from BIOL 101 at UNC. Gene Expression/Test 3 Tuesday, March 03, 2009 9:30 AM 1. Helicase molecule o Protein made on ribosome o In cytoplasm. Sometime cell needs sometime
An important new study has revealed the clearest picture yet of precisely how much measurement variation influences gene expression profiles of breast cancer.
Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA micro- array. Comparison of expression levels arising from co- hybridized samples is achieved by taking ratios of average expression levels for individual genes. The present paper concerns image processing to automatically segment digitized micro-arrays and measure median gene expression levees across cDNA target sites. The main difficulty arises from determination of the target site when signal intensity is low. Segmentation must be accomplished for target sites that can possess highly unstable geometry and consist of a relatively small number of pixels. Segmentation must also be computationally efficient. The present paper proposes a nonparametric statistical method that separates target site from local background using the Mann-Whitney test ...
Jurasek JV, Raposa LB, Gubicskóné Kisbenedek A et al.. Táplálkozástudományi és Dietetikai Intézet, Pécsi Tudományegyetem, Egészségtudományi Kar Pécs.. Orvosi hetilap. Mar 2017.. Nowadays, the food industry more often uses different type of additives during the food production.Our aim was to examine the monosodium-glutamates effect (in animal experiment) on DNA-methyltransferases in gene expression patterns of mRNA levels.In the investigation we used 24 (n=24) CD1 type female mice. The animals were fed with different equivalent human doses of the tested substance. After autopsy, mRNA was isolated from different tissues (lung, liver, kidney, spleen). DNMT1, DNMT3A and DNMT3B levels were determined by Quantitative Real-Time PCR.DNMT1 significantly suppressed the gene expression in all the three treated groups ( ...
We demonstrate here that SMART PCR-amplified cDNAs arrayed on a nylon membrane are suitable for high-throughput tissue expression profiling when starting biological materials are limited. We show that SMART cDNA accurately reflects gene expression patterns found in total RNA by comparing the express …
The ability of cells to control the expression of genes is important for cell survival. Nutrients can alter the gene expression that controls levels of bioactive proteins. Herbs alter gene activity in various ways. Gene transcription, the most important, is the initial step in gene expression. The pregnane X receptor (PXR receptor) is the target […]. View Post ...
TimesVector is a triclustering tool for clustering time-series data that comprises multiple conditions, or phenotypes. It identifies gene clusters that exhibit distinctly similar or different gene expression patterns among the comparing sample conditions. For example, for a time-series data set of five different strains of yeast, each data sampled at 3 time points, TimesVector will search for gene clusters where a specific strain shows a distinctively different gene expression pattern from the others.. As an example result, 33 genes in cluster 25 were significantly induced at day 5 (D5) during fermentation, only in the DV10 yeast strain (data from GSE11651) ...
The secretory protein Reg3γ (also known as PAP III) is expressed in several tissues, and its expression is induced in various injuries and inflammatory conditions in gastrointestinal tract (Iovanna et al. 1992; Ogawa et al. 2003), skeletal muscle (Klasan et al. 2014) as well as in central and peripheral nervous system (Namikawa et al. 2005, 2006; Ampo et al. 2009; Kawahara et al. 2011; Klasan et al. 2014). However, almost nothing is known about cardiac Reg3γ gene expression, although increased Reg3γ expression has been repeatedly identified in cardiac gene expression profiling screens (Rysä et al. 2005; Tenhunen et al. 2006a; Watanabe et al. 2008; Liu et al. 2009). In this study, we are first to characterize the expression and regulation of Reg3γ in the heart. We found that Reg3γ gene expression is rapidly up‐regulated by post‐MI remodeling and Ang II in the adult rat heart in vivo. We showed that losartan treatment reduced left ventricular Reg3γ gene expression levels activated by ...
The general goal of the technique is similar to the DNA microarray. However, SAGE is a sequence-based sampling technique. Observations are not based on hybridization, which result in more qualitative, digital values. In addition, the mRNA sequences do not need to be known a priori, so genes or gene variants which are not known can be discovered. Microarray experiments are much cheaper to perform, so large-scale studies do not typically use SAGE. Quantifying gene expressions is more exact in SAGE because it involves directly counting the number of transcripts whereas spot intensities in microarrays fall in non-discrete gradients and are prone to background noise. ...
Renal Tissue Thawed for 30 Minutes Is Still Suitable for Gene Expression Analysis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
BioXpedias Technologies for Gene Expression Profiling offers RNA analyses of samples, using technologies that are time-, cost-, and sample-effective.
Gene expression analysis market will surpass more than US$ 4,500 million revenues towards the end of the forecast period, 2017-2022.
Gene editing with the TUNR system allows you to obtain intermediates with high, medium and low reduction of gene expression with the same targeting quality as for knock-out. my notice earlier was an early April fools joke. Anyway, I apologize to Ron Edgar of the Gene Expression Omnibus, as a concerned reader emailed him. Ill try to think of something more innocuous next time. I should have just made up a fake organization ...
Learn how to prepare RNA for gene expression analysis from cultured cells in 7 minutes. The Cells-to-CT 1-Step TaqMan Kit is a simple, quick alternative to traditional RNA extraction.
The RNAscope® and BaseScope™ ISH assays can both be applied as methods to detect gene expression with morphological context in a multitude of mouse models.
Analysis of circulating tumor cells (CTCs) in a mouse model of pancreatic cancer identified distinct patterns of gene expression in several groups of CTCs, including significant differences from the primary tumor that may contribute to the ability to generate metastases.
"The oldest science blog of all? , Gene Expression , Discover Magazine". 2010-08-24. Retrieved 2012- ...
Gene Expression. Retrieved 3 March 2011. "Edge: AN EPIDEMIOLOGY OF REPRESENTATIONS: A Talk with Dan Sperber". Edge. Retrieved 3 ... Richerson, Peter J.; Boyd, Robert (2008). Not by genes alone: how culture transformed human evolution. Chicago, Il.: University ...
"Larry Wall, Fundamentalist, non-Creationist, programmer". Gene Expression. Wall, Larry (March 3, 1999), Perl, the first ...
In addition, it was shown in one study involving a Finnish population that the lactase gene has a higher expression when G− ... "Lactose tolerance/intolerance". Gene Expression. January 19, 2004. Retrieved 2008-01-31. (Outram et al. 2009 Anke Liebert (2014 ... This is evident in examining the mammalian lactase gene (LCT), which expression decreases after the weaning stage, resulting in ... They are all located in a region of the gene MCM6 upstream of LCT. This region is considered as an enhancer region for the ...
Furthermore, genes are often flanked by several binding sites for distinct transcription factors, and efficient expression of ... Transcription factors can also be used to alter gene expression in a host cell to promote pathogenesis. A well studied example ... Transcription factors are essential for the regulation of gene expression and are, as a consequence, found in all living ... Transcription factors use a variety of mechanisms for the regulation of gene expression. These mechanisms include: stabilize or ...
Gene Expression. 2003-02-23. Retrieved 2008-07-15. Wang, Wendy (2012-02-16). "The Rise of Intermarriage: Rates, Characteristics ... A case of gene culture co‐evolution?". Ethnic and Racial Studies. 9 (1): 87-113. doi:10.1080/01419870.1986.9993516. ISSN 0141- ...
Jardim DL, da Cunha AF, Duarte Ada S, dos Santos CO, Saad ST, Costa FF (May 2005). "Expression of Sara2 human gene in erythroid ... SAR1 gene homolog B (S. cerevisiae), also known as SAR1B, is a protein which in humans is encoded by the SAR1B gene. SAR1B ... Gene Expression. 10 (5-6): 231-42. PMID 12450215. Takai Y, Sasaki T, Matozaki T (January 2001). "Small GTP-binding proteins". ... "Entrez Gene: SAR1B SAR1 gene homolog B (S. cerevisiae)". He H, Dai F, Yu L, She X, Zhao Y, Jiang J, Chen X, Zhao S (2002). " ...
"Gene Expression , Learn Science at Scitable". Retrieved 2017-07-29. "tissue - definition of tissue in English ... Proteins dictate cell function and gene expression, a cell is able to self-regulate by the amount of proteins produced. However ...
Lin VK, Wang D, Lee IL, Vasquez D, Fagelson JE, McConnell JD (Aug 2000). "Myosin heavy chain gene expression in normal and ... Weir L, Chen D (1997). "Characterization of the nonmuscle myosin heavy chain IIB promoter: regulation by E2F". Gene Expression ... Targeted gene disruption of NM-IIB resulted in approximately 65% embryonic lethality, and those that survived suffered from ... "Entrez Gene: MYH10 myosin, heavy chain 10, non-muscle". "Protein sequence of human MYH10 (Uniprot ID: P35580)". Cardiac ...
Maraia, RJ; Intine, RV (2002). "La protein and its associated small nuclear and nucleolar precursor RNAs". Gene expression. 10 ... Binding between 5S rRNA and TFIIIA serves to both repress further transcription of the 5S RNA gene and stabilize the 5S RNA ... Pelham, HRB; Brown DD (1980). "A specific transcription factor that can bind either the 5S RNA gene or 5S RNA". Proc. Natl. ... Organelle genomes encode SSU and LSU rRNAs without exception, yet the distribution of 5S rRNA genes (rrn5) is most uneven. Rrn5 ...
"Conservative atheists not rare in South Korea?". Gene Expression. Retrieved 24 September 2015. Alex Buchan. "Inside the Dungeon ...
... is a prostatic tumor suppressor gene. NKX3-1 is an androgen-regulated, prostate-specific homeobox gene whose expression ... Gene Expression. 10 (5-6): 201-11. PMID 12450213. Gelmann EP, Bowen C, Bubendorf L (May 2003). "Expression of NKX3.1 in normal ... The gene which they symbolized NKX3-1 encoded a 234-amino acid polypeptide with greatest homology to the Drosophila NK3 gene. ... NKX3-1 expression was visualized using Fluorescence microscopy, utilizing GFP-NKX3-1 in the nucleus. NKX3-1 expression acts as ...
Khan, Razib (7 October 2017). "The Tibeto-Burman And Austro-Asiatic Ancestry Of Bengalis". Gene Expression. Retrieved 19 ... people of Bangladesh and West Bengal and the Sinhalese people of Sri Lanka possess some Austro-Asiatic ancestry via gene flow ...
Gene Expression". Gene Expression. 2013-08-04. Retrieved 2017-03-10. "What the Harappa Ancestry Project has resolved - Gene ... Expression". Gene Expression. 2013-08-04. Retrieved 2017-03-17. Zack (7 February 2011). "HapMap Gujaratis". Harappa Ancestry ... It also confirms low-level of female gene inflow within the group. The study illustrates impact of culture marriage practice on ... But about 60% of their maternal gene pool originates from South Asian haplogroups, which is just 7% in Iranians. Parsis have ...
Li J. J; Biggin M. D. (2015). "Gene expression. Statistics requantitates the central dogma". Science. 347 (6226): 1066-1067. ... Variation in methylation states of DNA can alter gene expression levels significantly. Methylation variation usually occurs ... This process causes the intein sequence to be copied from the original source gene to the intein-free gene. This is an example ... This is a case of a protein changing its own primary sequence from the sequence originally encoded by the DNA of a gene. ...
Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located ... by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression ... Gene Expression. 5 (4-5): 217-28. PMID 8723388. Kasof GM, Goyal L, White E (Jun 1999). "Btf, a novel death-promoting ... This gene also has a pseudogene. TARBP2 has been shown to interact with Protein kinase R and RBM14. GRCh38: Ensembl release 89 ...
Gene Expression. 8 (4): 207-17. PMID 10794523. Cuppen E, van Ham M, Wansink DG, de Leeuw A, Wieringa B, Hendriks W (Apr 2000 ... Thyroid receptor-interacting protein 6 is a protein that in humans is encoded by the TRIP6 gene. This gene is a member of the ... "Entrez Gene: TRIP6 thyroid hormone receptor interactor 6". Xu J, Lai YJ, Lin WC, Lin FT (Mar 2004). "TRIP6 enhances ... Genes & Development. 18 (20): 2518-28. doi:10.1101/gad.322404. PMC 529539 . PMID 15489293. Li L, Bin LH, Li F, Liu Y, Chen D, ...
Gene Expression. Retrieved 22 February 2006. Martin, N (2001). "Retrieving the 'eu' from eugenics". Nature. 414 (6864): 583. ... He argues that due to the advance of medicine, selection against those with poor genes for health was relaxed. Lynn examined ... Bibliography Cavalli-Sforza, L. L., Menozzi, P., & Piazza, A. (1994). The history and geography of human genes. Princeton, New ... genes, and IQ at the center of discussions surrounding inequality. Through his own writings and those published by his Ulster ...
... and expression of a functional gene". Gene. 126 (2): 227-35. doi:10.1016/0378-1119(93)90371-9. PMID 8482538. Garson K, Duhig T ... This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence ... Garson K, Duhig T, Fried M (1997). "Tissue-specific processing of the Surf-5 and Surf-4 mRNAs". Gene Expression. 6 (4): 209-18 ... The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded ...
Corre S, Galibert MD (Jan 2006). "[USF as a key regulatory element of gene expression]". Médecine Sciences. 22 (1): 62-7. doi: ... USF2 has been shown to interact with USF1 (human gene), PPRC1 and BRCA1. The USF2 gene is repressed by the microRNA miR-10a. ... Upstream stimulatory factor 2 is a protein that in humans just encoded by the USF2 gene. This gene encodes a member of the ... Gene Expression. 2 (3): 231-40. PMID 1450663. Blanar MA, Rutter WJ (May 1992). "Interaction cloning: identification of a helix- ...
Gene Expression Patterns. 7 (8): 827-36. doi:10.1016/j.modgep.2007.08.001. PMC 3360971 . PMID 17916448. Jiang SW, Wu K, ... Transcriptional enhancer factor TEF-5 is a protein that in humans is encoded by the TEAD3 gene. This gene product is a member ... "Entrez Gene: TEAD3 TEA domain family member 3". Bürglin, TR (Jul 1991). "The TEA domain: a novel, highly conserved DNA-binding ... Mann CJ, Osborn DP, Hughes SM (Oct 2007). "Vestigial-like-2b (VITO-1b) and Tead-3a (Tef-5a) expression in zebrafish skeletal ...
Gimeno L, Brûlet P, Martínez S (Aug 2003). "Study of Fgf15 gene expression in developing mouse brain". Gene Expression Patterns ... The mouse Fgf15 gene is syntenic with the human FGF19 gene. FGF15 and FGF19 proteins share about 50% amino acid identity, are ... Fibroblast growth factor 15 is a protein in mouse encoded by the Fgf15 gene. It is a member of the fibroblast growth factor ( ... McWhirter JR, Goulding M, Weiner JA, Chun J, Murre C (Sep 1997). "A novel fibroblast growth factor gene expressed in the ...
"Expression of Hairy/Enhancer of Split genes, Hes1 and Hes5, during murine nephron morphogenesis". Gene Expression Patterns. 4 ( ... Yamada K, Miyamoto K (September 2005). "Basic helix-loop-helix transcription factors, BHLHB2 and BHLHB3; their gene expressions ... "ortholog_gene_79365[group] - Gene - NCBI". Retrieved 2017-04-13. "Transcript: BHLHE41-001 ( ... "BHLHE41 Symbol Report , HUGO Gene Nomenclature Committee". Retrieved 2017-04-13. "Gene: BHLHE41 ( ...
... gene in the mouse germ cells". Gene Expression Patterns. 6 (6): 607-12. doi:10.1016/j.modgep.2005.11.013. PMID 16434236. Mohan ... January 2002). "Augmented expression of P-gp/multi-drug resistance gene by all-trans retinoic acid in monocytic leukemic cells ... Grskovic B, Pollaschek C, Mueller MM, Stuhlmeier KM (June 2006). "Expression of hyaluronan synthase genes in umbilical cord ... "Bisphenol A causes malformation of the head region in embryos of Xenopus laevis and decreases the expression of the ESR-1 gene ...
"Gene Expression Omnibus". NCBI Geo. NCBI Geo. Retrieved 9 May 2015. "BioGPS". BioGPS. BioGPS. Retrieved 9 May 2015. "Basic ... which is a set of similar genes that are formed by duplication of an original gene, is around 120 genes. Gene duplication, ... The TMEM251 gene is located on human chromosome 14, at 14q32.12, on the plus strand. The gene size is 1,277 base pairs. It ... Over-expression of the TMEM251 gene has not been linked as a causal factor in any of these disease states The conditions under ...
doi:10.1007/978-3-540-69185-3. ISBN 978-3-662-21625-5. "NHLRC2". Gene Expression Omnibus. Retrieved 4 Apr 2015. "NHLRC2". ... NCBI GEO Profiles detailed several conditions under which NHLRC2 expression is increase in comparison to base expression levels ... The full gene spans 62,533 base pairs (bp). Eleven exons are transcribe in the protein-coding mRNA. There is a second, less ... NHL Repeat Containing Protein 2, or NHLRC2, is a protein encoded by the NHLRC2 gene . NHLRC2 has also been referred to as Novel ...
Gene Expression Patterns. 4 (3): 309-14. doi:10.1016/j.modgep.2003.10.004. PMID 15053980. Cheng J, Kapranov P, Drenkow J, Dike ... "Entrez Gene: SSPO SCO-spondin homolog (Bos taurus)". Gobron S, Creveaux I, Meiniel R, Didier R, Dastugue B, Meiniel A (January ... SCO-spondin is a protein that in humans is encoded by the SSPO gene. SCO-spondin is secreted by the subcommissural organ, and ... Gonçalves-Mendes N, Simon-Chazottes D, Creveaux I, Meiniel A, Guénet JL, Meiniel R (July 2003). "Mouse SCO-spondin, a gene of ...
Gene Expression Patterns. 4 (3): 309-14. doi:10.1016/j.modgep.2003.10.004. PMID 15053980. Huh MS, Todd MA, Picketts DJ (April ... SCO-spondin and F-spondin share a similar pattern of expression in the floor plate, flexural organ and subcommissural organ and ... Herz J (March 2001). "The LDL receptor gene family: (un)expected signal transducers in the brain". Neuron. 29 (3): 571-81. doi: ... Gonçalves-Mendes N, Blanchon L, Meiniel A, Dastugue B, Sapin V (May 2004). "Placental expression of SCO-spondin during mouse ...
Yang DH, Moss EG (Dec 2003). "Temporally regulated expression of Lin-28 in diverse tissues of the developing mouse". Gene ... "Vascular gene expression and phenotypic correlation during differentiation of human embryonic stem cells". Developmental ... Lin-28 homolog A is a protein that in humans is encoded by the LIN28 gene. LIN28 encodes an RNA-binding protein that binds to ... "Entrez Gene: LIN28 lin-28 homolog (C. elegans)". Tsialikas J, Romer-Seibert J (Jul 2015). "LIN28: roles and regulation in ...
NRL expression leads to the rod fate. NR2E3 further restricts cells to the rod fate by repressing cone genes. RORbeta is needed ... photoreceptor gene expression; and lastly axonal growth, synapse formation and outer segment growth. Early Notch signaling ... CRX further defines the photoreceptor specific panel of genes being expressed. ... "Induction of photosensitivity by heterologous expression of melanopsin". Nature. 433 (7027): 745-9. Bibcode:2005Natur.433..745Q ...
基因劑量(Gene dosage)會對人類的表現型產生龐大的影響,對於染色體中造成疾病的複寫、省略與分裂等現象的形成擁有一定的角色。例如唐氏症患者(21號染色體為三體)有較高的比率得到阿茲海默症,可能是因為與阿茲海默症有關的類澱粉前趨蛋白基因(位在21號 ... 人類基因組含有許多不同的調控序列,並以此來控制基因表現。這
... appearance-observable traits caused by the expression of a condition's genes. The features of craniosynostosis' particular ... in FGFR genes) and mutations that lead to loss of function (in TWIST genes).[38][39] Craniosynostosis is therefore likely the ... Gene Loeys-Dietz syndrome wide-set eyes • split uvula or cleft palate • arterial tortuosity • aortic root dilatation • ... In terms of syndromic craniosynostosis not only do FGFR3 and TWIST genes feature, but also FGFR1 and in particular FGFR2, which ...
For her, the greatest taboos in life were crying in front of people and showing expressions of pain.[5] She reminisced, "I did ... Gene Weltfish. This pamphlet was intended for American troops and set forth, in simple language with cartoon illustrations, the ... Societies all have social norms that they follow; some allow more expression when dealing with death, such as mourning, while ...
Manohar CF, Salwen HR, Furtado MR, Cohn SL (1996). "Up-regulation of HOXC6, HOXD1, and HOXD8 homeobox gene expression in human ... This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors ... consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on ... This article on a gene on human chromosome 2 is a stub. You can help Wikipedia by expanding it.. *v ...
"Entrez Gene: NMT1 N-myristoyltransferase 1". Rajala RV, Datla RS, Moyana TN, et al. (2000). "N-myristoyltransferase". Mol. Cell ... Mumby SM, Heukeroth RO, Gordon JI, Gilman AG (1990). "G-protein alpha-subunit expression, myristoylation, and membrane ... is an enzyme that in humans is encoded by the NMT1 gene. GRCh38: Ensembl release 89: ENSG00000136448 - Ensembl, May 2017 GRCm38 ...
These gene products are important in determining cell fates during normal development and in maintaining homeostasis, or they ... Ji J, Yamashita T, Wang XW (2011). "Wnt/β-catenin signaling activates microRNA-181 expression in hepatocellular carcinoma". ... Mutations in genes encoding these proteins can lead to inactivation of cadherin cell adhesions and elimination of contact ... Mutations in catenin genes can cause loss of contact inhibition that can promote cancer development and tumor formation. ...
Such expression QTLs (eQTLs) describe cis- and trans-controlling elements for the expression of often disease-associated genes. ... In a recent development, classical QTL analyses were combined with gene expression profiling i.e. by DNA microarrays. ... Several genes factor into determining a person's natural skin color, so modifying only one of those genes can change skin color ... The DNA sequence of any genes in this region can then be compared to a database of DNA for genes whose function is already ...
The eyes are somewhat large with an alert expression. Any eye colour is allowed. The coat is short and fine, usually straight ... Registered rabbits are maintained in an official gene bank registry.[3] 4 week old Gotland rabbits ...
"Ingenuity IPA Software - Pathway Analysis, miRNA, NGS, RNA-Seq, Microarrays, Gene Expression, Biomarkers". Ingenuity Systems. ... "Sigma-Aldrich Launches Your Favorite Gene Powered by Ingenuity, Sigma-Aldrich Co" (Press release). Sigma-Aldrich Co., Inc. ... IPA also lets researchers search for information on genes, proteins, chemicals, drugs, and reagents. Resulting information can ... IPA helps researchers analyze data derived from expression and SNP microarrays, proteomics experiments, and small-scale ...
positive regulation of gene expression. • cell-cell signaling. • hormone-mediated signaling pathway. • follicle-stimulating ... 1989). "Expression of biologically active human follitropin in Chinese hamster ovary cells". J. Biol. Chem. 264 (9): 4769-75. ... 1999). "Characterization of single-nucleotide polymorphisms in coding regions of human genes". Nat. Genet. 22 (3): 231-8. PMID ... 1997). "Delayed puberty and hypogonadism caused by mutations in the follicle-stimulating hormone beta-subunit gene". N. Engl. J ...
"Cross" (Eugene Novotney). The clave rhythm and clave concept have been used in some modern art music ("classical") compositions ... they are all expressions of the same rhythmic principles. The three key patterns are found within a large geographic belt ... Novotney, Eugene N. (1998) "Thesis: The 3:2 Relationship as the Foundation of Timelines in West African Musics" (registration ... Another clave-based composition that has "gone global" is the snare drum suite "Cross" by Eugene D. Novotney. ...
Transformation is a complex developmental process requiring energy and is dependent on expression of numerous genes. In S. ... It has a core set of 1553 genes, plus 154 genes in its virulome, which contribute to virulence and 176 genes that maintain a ... For instance, the Xisco gene was recently described as a biomarker for PCR-based detection of S. pneumoniae and differentiation ... gene for identification of Streptococcus pneumoniae isolates". Diagnostic Microbiology and Infectious Disease. 90 (4): 248-250 ...
"MN1 affects expression of genes involved in hematopoiesis and can enhance as well as inhibit RAR/RXR-induced gene expression". ... MN1 is a gene found on human chromosome 22, with gene map locus 22q12.3-qter.[5] Its official full name is meningioma ( ... in myeloproliferative disorders results in fusion of the ETS-like TEL gene on 12p13 to the MN1 gene on 22q11". Oncogene. 10 (8 ... in myeloproliferative disorders results in fusion of the ETS-like TEL gene on 12p13 to the MN1 gene on 22q11". Oncogene. 10 (8 ...
2009). «Fibulin-4 regulates expression of the tropoelastin gene and consequent elastic-fibre formation by human fibroblasts». ... Ontologia do gene. Função molecular. •extracellular matrix structural constituent. •protein binding. •extracellular matrix ... Rosenbloom J (1984). «Elastin: relation of protein and gene structure to disease». Lab. Invest. 51 (6): 605-23. PMID 6150137. ... 2004). «A novel elastin gene mutation resulting in an autosomal dominant form of cutis laxa». Arch Dermatol. 140 (9): 1135-9. ...
This triggers the expression of interferon-stimulated genes, which code for proteins with antiviral properties.[51] EBOV's V24 ... encoded by the L gene, partially uncoats the nucleocapsid and transcribes the genes into positive-strand mRNAs, which are then ... Ebolaviruses contain single-stranded, non-infectious RNA genomes.[46] Ebolavirus genomes contain seven genes including 3'-UTR- ... differ in sequence and the number and location of gene overlaps. As with all filoviruses, ebolavirus virions are filamentous ...
Early examples of artistic expression, such as the Venus of Tan-Tan and the patterns found on elephant bones from Bilzingsleben ... "Chimps, Humans 96 Percent the Same, Gene Study Finds". Retrieved 23 December 2013 ...
More reference expression data. Gene ontology. Molecular function. • ion channel activity. • benzodiazepine receptor activity. ... "Identification of significant association and gene-gene interaction of GABA receptor subunit genes in autism". Am. J. Hum. ... Gamma-aminobutyric acid receptor subunit alpha-4 is a protein that in humans is encoded by the GABRA4 gene.[5][6] ... to human chromosome 4 defines an alpha 2-alpha 4-beta 1-gamma 1 gene cluster: further evidence that modern GABAA receptor gene ...
The chloroplast is mostly under nuclear control, though chloroplasts can also give out signals regulating gene expression in ... Gene content and protein synthesisEdit. The chloroplast genome most commonly includes around 100 genes[7][10] which code for a ... 3 Gene content and protein synthesis *3.1 Chloroplast genome reduction and gene transfer ... Chloroplasts may contain 60-100 genes whereas cyanobacteria often have more than 1500 genes in their genome.[27] Contrarily, ...
The video exploits Tilton's facial expressions and preaching style. The original video contained no title screen and was ... Some of Tilton's fundraising letters were written by Gene Ewing, who heads a multimillion-dollar marketing empire writing ...
"Expression profile of active genes in human periodontal ligament and isolation of PLAP-1, a novel SLRP family gene". Gene. 275 ... "Expression pattern and gene characterization of asporin. a newly discovered member of the leucine-rich repeat protein family". ... "Entrez Gene: ASPN asporin". مؤرشف من الأصل في 05 ديسمبر 2010. الوسيط ,مسار أرشيف=. تم تجاهله (مساعدة); الوسيط ,تاريخ أرشيف=. تم ... Yamada S، Ozawa Y، Tomoeda M، Matoba R، Matsubara K، Murakami S (May 2006). "Regulation of PLAP-1 expression in periodontal ...
... organization of the gene, chromosome localization, and functional expression of cDNA clones". Biochemistry. 30 (44): 10640-6. ... and cytokine expression,[58] Reciprocally, cytokines may induce expression of SP and its NK1R.[59][60] In this sense, for ... "C/EBPbeta couples dopamine signalling to substance P precursor gene expression in striatal neurones". Journal of Neurochemistry ... SP initiates expression of almost all known immunological chemical messengers (cytokines).[19][20][21] Also, most of the ...
2002). "Regulated expression of the apolipoprotein E/C-I/C-IV/C-II gene cluster in murine and human macrophages. A critical ... Apolipoprotein (apo)C4 gene is a member of the apolipoprotein C gene family. It is expressed in the liver and has a predicted ... Apo C4 is a 3.3-kb gene consisting of 3 exons and 2 introns; it is located 0.5 kb 5' to the APOC2 gene.[5] ... This article on a gene on human chromosome 19 is a stub. You can help Wikipedia by expanding it. *v ...
Fu X, Zhu ZH, Wang YQ, Wu GC (January 2007). "Regulation of proinflammatory cytokines gene expression by nociceptin/orphanin FQ ...
These five proteins directly control the timing of expression of over 200 genes. The five master regulatory proteins are ... The Caulobacter CB15 genome has 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes.[7] The genome ... "DnaA couples DNA replication and the expression of two cell cycle master regulators". The EMBO Journal. 25 (2): 346-56. doi ... contains multiple clusters of genes encoding proteins essential for survival in a nutrient-poor habitat. Included are those ...
NRL expression leads to the rod fate. NR2E3 further restricts cells to the rod fate by repressing cone genes. RORbeta is needed ... photoreceptor gene expression; and lastly axonal growth, synapse formation and outer segment growth. ... CRX further defines the photoreceptor specific panel of genes being expressed. ... "Induction of photosensitivity by heterologous expression of melanopsin". Nature. 433 (7027): 745-9. Bibcode:2005Natur.433..745Q ...
... mechanical modulation of cell shape and gene expression". Tissue Eng Part A. 14 (9): 1573-80. doi:10.1089/ten.tea.2008.0113. ... General Trsp gene deletion is lethal to the embryo. The results of this research was used as a model for Kashin-Beck disease. ... General gene knock out of the TGF-β resulted in death. Conditional inactivation of TGF-βr2 of osteochondroprogenitor cells in ... Sox9 blocked osteochondroprogenitor cells were found to express osteoblast marker genes, reprogramming the cells into the ...
"Expression of an antisense prosystemin gene in tomato plants reduces resistance toward Manduca sexta larvae". Proceedings of ... by adding antisense genes to silence the native gene or by adding extra copies of the native gene.[45][46] ... "Expression of a synthesized gene encoding cationic peptide cecropin B in transgenic tomato plants protects against bacterial ... A genetically modified tomato, or transgenic tomato, is a tomato that has had its genes modified, using genetic engineering. ...
... it is possible that it regulates expression of genes other than thyroid-specific. Several known tumor suppressor genes like ... Paired box gene 8, also known as PAX8, is a protein which in humans is encoded by the PAX8 gene.[5] ... Down regulation of the PAX gene expression inhibits cell growth and induces apoptosis. This could be a possible avenue for ... This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode ...
Pays, E. (2005). "Regulation of antigen gene expression in Trypanosoma brucei". Trends Parasitol. 21 (11): 517-20. doi:10.1016/ ... According to this model, new genes are created by non-adaptive processes, such as by random gene duplication. These novel ... All changes in the gene frequencies of populations--and quite often in the traits those genes influence--are by definition ... how new alternative spliced isoforms of genes arise, how gene scrambling in ciliates evolved, and how pervasive pan-RNA editing ...
2001). „Cyclical regulation of GnRH gene expression in GT1-7 GnRH-secreting neurons by melatonin". Endocrinology. 142 (11): ... 2000). „Melatonin receptor mRNA expression in human granulosa cells". Mol. Cell. Endocrinol. 156 (1-2): 107-10. PMID 10612428. ... 2004). „The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome ...
... induced a 50-100-fold increased in IL-2 gene expression and secretion. High levels of IL-2 gene expression could also be ... IL-2 gene expression and T-cell proliferation induced by CD3 MoAb plus PMA or calcium ionophore plus PMA were completely ... In contrast, IL-2 gene expression and T-cell proliferation induced by CD28 MoAb plus PMA were unaffected by cyclosporine. The ... Two distinct mechanisms of interleukin-2 gene expression in human T lymphocytes ...
DNA actively 58-49-1 biological activity down-regulates the gene expression of LL37 in monocytes and epithelial cells. ... DNA actively down-regulates the gene expression of LL37 in monocytes and. Posted By: URAT1 inhibitor June 28, 2017 ... Be the first to comment on "DNA actively down-regulates the gene expression of LL37 in monocytes and". ... DNA actively 58-49-1 biological activity down-regulates the gene expression of LL37 in monocytes and epithelial cells. ...
மரபணு வெளிப்பாடு (Gene expression) என்பது மரபணுவில் இருக்கும் தகவல்கள், தொழிற்படக்கூடிய மரபணு உற்பத்திப்பொருளாக (gene product) ...
Among genes coding for eCB catabolic enzymes, expression of MGLL was lower in tumour tissue while PTGS2 was increased. It is ... Altered mRNA Expression of Genes Involved in Endocannabinoid Signalling in Squamous Cell Carcinoma of the Oral Tongue. ... expression of genes coding for the components of the eCB system in tumour and non-malignant samples from SCCOT patients. ... Expression of NAPEPLD and PLA2G4E, coding for eCB anabolic enzymes, was higher in the tumour tissue than in non-malignant ...
Its expression gives: The k-expressions of gene expression programming correspond to the region of genes that gets expressed. ... The chromosomes of gene expression programming are usually composed of more than one gene of equal length. Each gene codes for ... This kind of expression tree consists of the phenotypic expression of GEP genes, whereas the genes are linear strings encoding ... Because these parse trees are the result of gene expression, in GEP they are called expression trees. The genome of gene ...
Gene expression,state=autocollapse}}. *shows the template collapsed to the title bar if there is a {{navbar}}. , a {{sidebar}} ... Gene expression,state=collapsed}}. to show the template collapsed, i.e., hidden apart from its title bar ... Gene expression,state=expanded}}. to show the template expanded, i.e., fully visible ... Retrieved from "" ...
Gene set analysis demonstrated several major advantages over individual gene differential expression analysis. Gene sets are ... Gene expression profiling in cancer Spatiotemporal gene expression Transcriptomics Splice variant analysis "Microarrays ... gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global ... Gene expression profiling may become an important diagnostic test. The human genome contains on the order of 25,000 genes which ...
Gene Expression. Tag archives for Neandertal. Apartheid of Iberian Neandertals & modern humans. Posted by Razib Khan on March ...
The gene regulatory program of Acrobeloides nanus reveals conservation of phylum-specific expression Philipp H. Schiffer, ... Activity-dependent aberrations in gene expression and alternative splicing in a mouse model of Rett syndrome Sivan Osenberg, ... Minor zygotic gene activation is essential for mouse preimplantation development Ken-ichiro Abe, Satoshi Funaya, Dai Tsukioka, ... dCas9-targeted locus-specific protein isolation method identifies histone gene regulators Chiahao Tsui, Carla Inouye, Michaella ...
It must be genetic! You seem to have the same gene I do for having a great big love of cats! Ever since I was a baby, holding ...
Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols ... and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in ... DNA In silico Microarray PCR Polymerase Chain Reaction Promoter SNP Single Nucleotide Polymorphism gene expression gene ... Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene ...
The main interest of our lab is to understand the physical basis underlying molecular mechanisms of gene expression. ...
Recombinant DNA Approaches in the Investigation of Muscle Gene Expression. * Front Matter Pages 281-281 ... Alterations in Phenotype Expression of Muscle by Chronic Nerve Stimulation Dirk Pette, Annegard Heilig, Gary Klug, Heinz ... The gene product associated with the initial disturbance in skeletal muscle has also remained elusive up to now. Moreover, ... Phenotypic Expression & Molecular Markers for Muscle Diseases. * Front Matter Pages 23-23 ...
Identifying Differential Gene Expression You can now compare the gene expression values between two groups of data: CNS ... Use the following techniques to filter out these genes.. Remove gene expression data with empty gene symbols (in this example, ... Therefore an up-regulated gene in this example has higher expression in MD, and down-regulated gene has higher expression in ... Ctrl-click genes in the gene lists to label the genes in the plot. As seen in the volcano plot, genes specific for neuronal ...
An expression system refers to the factors that work together to yield a particular gene product such as a protein, ribozyme or ... In this sense, every cell in the human body is an expression system. However, the term usually refers to gene expression in the ... These configurations help form the expression system of the cell by regulating DNA replication and gene expression. Some of ... The expression system is made up of a gene, which is encoded by DNA, and the machinery needed to make mRNA from the DNA and ...
Gene expression is the process by which DNA is used to make proteins, which then go on to perform various important functions ... Gene Expression Mechanism. News-Medical, viewed 25 February 2021, ... Gene Expression Mechanism. News-Medical. 25 February 2021. , ... Gene Expression Mechanism. News-Medical. (accessed ...
The Plant Gene Expression Center is located in Albany, CA and is part of the Pacific West Area.. The Center Director is Sarah ... Characterization of Plant Architectural Genes in Maize for Increased Productivity * Conserved Genes and Signaling Networks that ... USDA-ARS, PLANT GENE EXP. CTR. 800 BUCHANAN STREET. Albany, CA 94710. ...
REgulation of MItochondrial gene eXpression), an Innovation Training Network funded under the highly competitive Marie ... a program that will contribute to elucidating the molecular mechanisms and pathways that regulate mitochondrial gene expression ... Mitochondrial gene expression International team led by the researcher on aging Aleksandra Trifunovic from Cologne wins Marie ... "The complex nature of mitochondrial gene expression that relies on two different genomes calls for a multidisciplinary approach ...
Gene Expression data sets used: * The original web site from which the rat CNS data (Wen et al. 1998) was available for ... Due to popular demand, we are making the subset of 384 genes we used in Ka Yees dissertation available (as text-delimited file ... We are also making the subset of 237 genes (corresponding to 4 MIPS categories) used in Ka Yees dissertation availabel (as ...
Insulin-regulated gene expression.. OBrien RM1, Streeper RS, Ayala JE, Stadelmaier BT, Hornbuckle LA. ... Insulin regulates the expression of more than 150 genes, indicating that this is a major action of this hormone. At least eight ... Our results suggest that this model is consistent with the action of insulin on transcription of the gene encoding IGFBP-1 but ... All of these IRSs mediate stimulatory effects of insulin on gene transcription. In contrast, an element with the consensus ...
For other gene sets, such as transcription factors or proteolytic genes, the number of genes whose expression was detected in ... The expression of a given gene in a given sample is approximated by the projection of the gene vector onto the direction of the ... We used Gene Ontology to select genes related to transcriptional activity. The list from Gene Ontology was curated by hand to ... To investigate the specificity and stability of lineage-specific gene expression, we included expression patterns of several ...
Regulation of MHC gene expression.. Ting JP1, Baldwin AS.. Author information. 1. Department of Microbiology and Immunology, ... The better characterization of proteins that interact with MHC class I and II promoters and the isolation of genes encoding ... New insights have also been gained in the understanding of MHC-associated genes. ... allow the functional analysis of these molecules in MHC gene regulation. The application of new techniques, such as genomic in ...
At the same time, if screening reveals that there is little or no impact on gene expression from a particular chemical, why not ... "The extra information we get from looking at gene expression could help us make more informed decisions about how harmful a ... This combination of genomics (gene expression) and environmental toxicology is a rapidly growing field, and will enable us to ... By looking at which genes are expressed (turned on and off) by the water flea in response to sub-lethal toxins, Dr. Vulpe can ...
The definition of retina-specific promoters will make possible targeted expression of heterologous and altered genes to the ... This application proposes to study the molecular basis of retinal development using the regulation of rhodopsin gene expression ... Studies on the regulation of expression of retina-specific genes, in addition to their importance at the basic level, will also ... have identified cis-acting DNA regulatory sequences that are capable of directing photoreceptor cell-specific gene expression. ...
Gene, Long Non-Coding RNA Expression Shift During Organ Development Genomeweb * Netzwerke der Genaktivität steuern die ... Gene expression across mammalian organ development. Last updated: Sat, 18 Jan 2020 11:57:55 GMT ... Networks of gene activity control organ development * Networks of Gene Activity Control Organ Development ... Networks of gene activity control organ development EurekAlert! * Netzwerke der Genaktivität steuern die Organentwicklung ...
Gene Expression. This blog is about evolution, genetics, genomics and their interstices. Please beware that comments are ...
title = {An incremental clustering of gene expression data},. booktitle = {in Proceedings of NABIC, Coimbatore},. year = {2009} ...
... a team of researchers from UNIGE measured the way mercury affects the gene expression of algae, even when its concentration in ... Mercury is altering gene expression By studying the RNA, UNIGE scientists are the first to demonstrate how mercury enters the ... The scientists measured the way mercury affects the gene expression of algae, even when its concentration in water is very low ... An alga that seems healthy from a physiological point of view appears altered when its genes expression is examined. The ...
This study demonstrates, for the first time in humans, that large changes in gene expression are likely an important mechanism ... From the article: Effect of dietary fatty acids on inflammatory gene expression in healthy humans, by Kelly L. Weaver, ... They then looked at the gene levels of immune signals and cytokines (protein immune messengers), that impact autoimmunity and ... including a signaling gene for a protein called PI3K, a critical early step in autoimmune and allergic inflammation responses. ...
Gene Expression follows the expression of protein-coding genes by looking at the environment of a gene in the nucleus, ... Genetics: Evolution and Gene Expression. Module code: NT4003. Evolutionary Genetics gives you an introductory overview of the ... This module will give a thorough understanding of the molecular mechanisms of gene expression and its control, in mammals, to ... By exploring evolutionary histories of gene regulation with particular reference to developmental and behavioural genes in ...
... including expression values, sample and feature metadata, and information about experimental methods and conditions. ... The ExpressionSet class is designed to contain data from a microarray gene expression experiment, ... Write expression values in ExpressionSet object to text file. expressions. Retrieve or set Expressions. DataMatrix object from ... The ExpressionSet class is designed to contain data from a microarray gene expression experiment, including expression values, ...
... GeneLab undertakes specific research and training projects, which require ... expertise in gene cloning, PCR, DNA sequencing, cDNA library construction, gene expression and other molecular methods. ...
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc ...
  • Two discrete mechanisms for induction of T-cell proliferation could be distinguished by examining cell cycle progression and the expression of the IL-2 gene. (
  • Interleukin-2 (IL-2) gene regulation was investigated in primary cultures of highly purified human peripheral blood CD28+T cells. (
  • DNA microarrays measure the relative activity of previously identified target genes. (
  • Microarrays contain oligonucleotide or cDNA probes for comparing the expression profile of genes on a genomic scale. (
  • Gene transcription in each of 4 cell strains was analyzed using high-density oligonucleotide DNA microarrays (U133A, Affymetrix™) and changes in the expression of selected genes were verified by real-time polymerase chain reaction. (
  • Large-scale biology projects such as the sequencing of the human genome and gene expression surveys using RNA-seq, microarrays and other technologies have created a wealth of data for biologists. (
  • Discover our large selection of Gene Expression & Exon Microarrays. (
  • This includes whole transcriptome gene expression for almost 30 different species, Exon microarrays to analyze splicing variants and gene expression microarrays with comprehensive content, including full LNCipedia databases to provide full coverage of the transcriptome in a single experiment. (
  • Affymetrix ATH1 GeneChip microarrays representing 22,810 genes were used to survey the transcriptome of seven Arabidopsis thaliana accessions in the presence and absence of exogenously applied salicylic acid (SA). (
  • This example shows how to identify differentially expressed genes from microarray data and uses Gene Ontology to determine significant biological functions that are associated to the down- and up-regulated genes. (
  • Determining if changes in gene expression are statistically significant between different conditions, e.g. two different tumor types, and determining the biological function of the differentially expressed genes, are important aims in a microarray experiment. (
  • You will use the t-test and false discovery rate to detect differentially expressed genes between two tumor types. (
  • In any test situation, two types of errors can occur, a false positive by declaring that a gene is differentially expressed when it is not, and a false negative when the test fails to identify a truly differentially expressed gene. (
  • We'll explore one of the main databases for RNA-seq expression data, the Sequence Read Archive (SRA), and then use an open-source suite of programs in R called BioConductor to process the raw reads from 4 RNA-seq data sets, to summarize their expression levels, to select significantly differentially expressed genes, and finally to visualize these as a heat map. (
  • The effects of a mixture of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) on transcriptional responses in the liver of medaka, Oryzias latipes, were investigated by identifying differentially expressed genes (DEGs). (
  • Between any pair of Arabidopsis accessions, we detected on average 2234 genes (ranging from 1428 to 3334) that were significantly differentially expressed under the conditions of this experiment, using a split-plot analysis of variance. (
  • Upward of 6433 genes were differentially expressed between at least one pair of accessions. (
  • First, which genes are differentially expressed among accessions or genotypes? (
  • Here we have investigated, at the mRNA level, expression of genes coding for the components of the eCB system in tumour and non-malignant samples from SCCOT patients. (
  • Genes contain the instructions for making messenger RNA (mRNA), but at any moment each cell makes mRNA from only a fraction of the genes it carries. (
  • Expression profiling experiments often involve measuring the relative amount of mRNA expressed in two or more experimental conditions. (
  • Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. (
  • The expression system is made up of a gene, which is encoded by DNA, and the machinery needed to make mRNA from the DNA and translate that into a protein. (
  • Gene Expression' follows the expression of protein-coding genes by looking at the environment of a gene in the nucleus, transcription, the processing of mRNA and production of multiple mRNA sequences from each gene, export of mRNA into the cytoplasm, proof-reading of mRNA, to the regulation by small RNA molecules. (
  • This topic will focus on the role of mRNA in the cell, platforms for profiling mRNA expression, the challenges in interpreting the data from these analyses, and the emerging clinical applications of gene expression measurements. (
  • Scientists - including co-first authors Maggie Chow, PhD, and Tiziano Pramparo, PhD, at UC San Diego - identified abnormal brain gene expression patterns using whole-genome analysis of mRNA levels and copy number variations from 33 autistic and control postmortem brain samples. (
  • If not blocked, it passes over the operator and reaches the protein-producing genes where it creates the mRNA that instructs the ribosomes to create the desired protein. (
  • Repressors bind with the operator and prevent RNA polymerase from proceeding to create mRNA by prohibiting access to the remainder of the protein-producing genes. (
  • Further studies have gone deeper into the gene expression profile of the disorder, demonstrating the key genes and miRNA-mRNA interactions that are involved in the pathways of Alzheimer's [6]. (
  • Expression of mRNA MMP-7 and mRNA TIMP-1 in non-small cell lung cancer. (
  • The relationship between the mRNA expression of proteolytic and steroidogenic enzymes in bovine placentomes was examined. (
  • R6/2 mice show decreased expression of transcription factor N10 mRNA. (
  • Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. (
  • The enzyme that synthesizes RNA (RNA polymerase) binds to promoter sequences along with transcription factors that either activate or repress promoters, therefore regulating gene expression. (
  • At least eight distinct consensus insulin response sequence (IRSs) have been defined through which insulin can regulate gene transcription. (
  • All of these IRSs mediate stimulatory effects of insulin on gene transcription. (
  • In contrast, an element with the consensus sequence T(G/A)TTT(T/G)(G/T), which we refer to as the phosphoenolpyruvate carboxykinase (PEPCK)-like motif, mediates the inhibitory effect of insulin on transcription of the genes encoding PEPCK, insulin-like-growth-factor-binding protein 1 (IGFBP-1), tyrosine aminotransferase and the glucose-6-phosphatase (G6Pase) catalytic subunit. (
  • The forkhead transcription factor FKHR has recently been shown to bind this PEPCK-like IRS motif and a model has been proposed in which insulin inhibits gene transcription by stimulating the phosphorylation and nuclear export of FKHR. (
  • Our results suggest that this model is consistent with the action of insulin on transcription of the gene encoding IGFBP-1 but not that of the G6Pase catalytic subunit. (
  • The better characterization of proteins that interact with MHC class I and II promoters and the isolation of genes encoding several of these transcription factors, such as H-2RIIBP/RXR beta, NK kappa B, I-kappa B, hXBP-1 and NF-Y, allow the functional analysis of these molecules in MHC gene regulation. (
  • We report here that an eight-ring polyamide targeted to a specific region of the transcription factor TFIIIA binding site interferes with 5S RNA gene expression in Xenopus kidney cells. (
  • Our results indicate that pyrrole-imidazole polyamides are cell-permeable and can inhibit the transcription of specific genes. (
  • Gene expression databases store information about the absolute or relative abundance of gene transcription products in various biological samples, such as cells from a particular tissue in a particular organism, or a particular cell line. (
  • Transcription and translation underlie gene expression. (
  • Most of the genome is selectively repressed, a property that is governed by the regulation of gene expression, mostly at the level of transcription (ie, the production of messenger RNA from the DNA). (
  • Total RNA was extracted from normal and neoplastic tissues and analyzed using a highly sensitive reverse transcription PCR assay with primers specific for the tyrosinase gene. (
  • The process of transcription, which is the synthesis of RNA from a DNA template, is where the regulation of the gene expression is most likely to occur. (
  • RNA polymerase must create RNA by moving along the chromosome and "reading" the genes in the process of transcription. (
  • Unlike prokaryotes, multiple gene-regulating mechanisms operate in the nucleus before and after RNA transcription, and in the cytoplasm both before and after translation. (
  • Early tests suggests sex-biased transcription factors play an important role in controlling the expression of sex-biased genes. (
  • This means that there might be sequences in the genes that are not expressed, which is indeed true for most genes. (
  • Sequence based techniques, like RNA-Seq, provide information on the sequences of genes in addition to their expression level. (
  • Within a gene or segment of DNA, certain sequences are referred to as promoters, enhancers and repressors. (
  • Preliminary transgenic mouse studies with fusion construct sconsisting of sequences upstream of the bovine rhodopsin gene ligated to the reporter gene lacZ (beta-galactosidase) have identified cis-acting DNA regulatory sequences that are capable of directing photoreceptor cell-specific gene expression. (
  • The Polymerase Chain Reaction (PCR), with its sensitive and selective amplification of specific nucleic acid sequences has become a research tool of almost unparalleled importance, with applications in, for example, cloning, gene expression analysis, DNA sequencing, genetic mapping and diagnostics. (
  • A method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated. (
  • 6. A method according to claim 3, wherein the specific excision signal sequences are LOX sequences and the second gene encodes CRE. (
  • 8. A method according to claim 3, wherein the plants are cotton plants, the transiently active promoter is a LEA promoter, the specific excision signal sequences are LOX sequences, the first gene encodes RIP, the second gene encodes CRE, and sequence that causes male sterility is the RIP gene linked to an anther specific promoter. (
  • Small molecules that target specific DNA sequences have the potential to control gene expression. (
  • The team focused on DNA methylation, which blocks the genetic sequences behind gene expression, and can "silence" the activity of a gene. (
  • In Cell on February 23, researchers report evidence that Neanderthal DNA sequences still influence how genes are turned on or off in modern humans. (
  • In this study, researchers analyzed RNA sequences in a dataset called the Genotype-Tissue Expression (GTEx) Project, looking for people who carried both Neanderthal and modern human versions of any given gene--one version from each parent. (
  • The genetic basis for disease is determined by the inheritance of genes containing specific sequences of deoxyribonucleic acid (DNA). (
  • In subsequent years in Germany he switched to research on mechanisms underpinning the control and regulation of gene expression and became a Department Head in one of Germany's Federal Research Institutes. (
  • This is due to alternative splicing, and also because cells make important changes to proteins through posttranslational modification after they first construct them, so a given gene serves as the basis for many possible versions of a particular protein. (
  • An expression system refers to the factors that work together to yield a particular gene product such as a protein, ribozyme or RNA particle. (
  • They then looked at the gene levels of immune signals and cytokines (protein immune messengers), that impact autoimmunity and allergy in blood cells and found that many key signaling genes that promote inflammation were markedly reduced compared to a normal diet, including a signaling gene for a protein called PI3K, a critical early step in autoimmune and allergic inflammation responses. (
  • The NIH-sponsored GENSAT (Gene Expression Nervous System Atlas) project has created close to 1,500 transgenic mouse lines expressing green fluorescent protein (GFP) reporters or DNA recombinases in specific neural and glial cell populations. (
  • Expression of the Ppp1-r17 protein in postnatal day 7 mouse cerebellum. (
  • 5. A method according to claim 3, wherein the first gene encodes ribosomal inhibitor protein (RIP). (
  • αComplementationEnabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experim. (
  • Topics covered include multiple sequence alignments, phylogenetics, gene expression data analysis, and protein interaction networks, in two separate parts. (
  • part determines how much protein of that gene is present in the cell. (
  • 29. Expression of myelin proteolipid protein in oligodendrocytes and transfected cells. (
  • An operon includes a special segment of genes that are regulators of the protein synthesis, but do not code for protein, called the promoter and operator. (
  • This type of overall control of protein synthesis is regulated by genes that control the packing density of histones. (
  • The ability to switch protein-coding genes 'on' and 'off' is a fundamental process shared by all cell types. (
  • All DNA is wrapped up into a protein straight-jacket, whose basic repeat, the 'nucleosome', must be countered in order to activate a gene. (
  • The 20,000 protein coding genes in the human cell produce a repertoire of ~100,000 proteins through the process of alternative splicing. (
  • To allow in vitro to in vivo extrapolation of the contribution of MRP2 toward hepatic disposition of its substrates, data on the interindividual variability of hepatic MRP2 protein expression are required. (
  • one has been identified as the myosin VIIa gene on chromosome 11q, and a second is a novel basement membrane protein called Usherin. (
  • A popular approach to class discovery involves grouping similar genes or samples together using one of the many existing clustering methods such the traditional k-means or hierarchical clustering, or the more recent MCL. (
  • At least two genetic pathways regulating organism longevity act by modifying gene expression. (
  • While the paper is not terribly conclusive in its definition of genes or pathways involved, (partially I suspect because of the limited time points examined and the late point in the examinations), this paper does however point in a direction that is useful to the retinal degeneration community. (
  • State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression. (
  • Now, in the most comprehensive genetic analysis to date, researchers from the Massachusetts Institute of Technology (MA, USA) have identified each of the genes expressed in individual brain cells of patients with AD, allowing them to determine the distinctive cellular pathways that are disturbed in each neuron or glial cell. (
  • Data Mining Tool software (Affymetrix) was used to separate genes in clusters based on their expression patterns over time. (
  • In gene expression data, a bicluster is a subset of the genes exhibiting consistent patterns over a subset of the conditions. (
  • Taking cotton as their study system over the past decade, the Wendel lab have applied a portfolio of newly emerging technologies to characterise patterns of gene expression in wild and cultivated species of diploid and allopolyploid cotton. (
  • Using this method, we aim to map the neuronal diversity of the somatosensory column based on the expression patterns of individual cells, which, if successful, will provide an exhaustive map of neuronal subtypes in the rodent neocortex. (
  • Schena M, Shalon D, Davis RW and Brown PO (1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. (
  • Patterns in gene expression include both the increase and/or decrease in the expression of genes (upregulation and downregulation) that are responsible for the production of RNA and proteins. (
  • We constrain the spline coefficients of genes in the same class to have similar expression patterns, while also allowing for gene specific parameters. (
  • The Gene Expression Markup Language (GEML) "is a file format for storing DNA microarray and gene expression data for chip patterns and chip scans ( profiles ). (
  • To fully study the sequence level polymorphisms that alter gene expression patterns, we include the full range of polymorphism types in our definition of ELPs. (
  • Such genes may be under selective pressure for altered expression patterns or the expression difference may be the result of neutral sequence variation. (
  • By comparing the pattern of nucleotide polymorphism to ELP variation, it may be possible to determine if nucleotide polymorphisms and expression level polymorphism show similar variation patterns, suggesting comparable levels of neutrality. (
  • This study demonstrates, for the first time in humans, that large changes in gene expression are likely an important mechanism by which these omega fatty acids exert their potent clinical effects. (
  • In response to a cellular perturbation, changes in gene expression take place that result in the expression of hundreds of gene products and the suppression of others. (
  • 10. Kainate-induced changes in gene expression in the rat hippocampus. (
  • see the Perspective by Rathmell and Newgard) demonstrate that such regulation helps link growth factor-stimulated increases in metabolism to appropriate changes in gene expression. (
  • Thus ACL may help cells link metabolic activity to changes in gene expression. (
  • The researchers say their study is the largest of its kind, and conclude that it "may shed light on the biological mechanism by which environmental influences regulate the activity of certain genes and in turn contribute to the development of ASD and related behavior traits. (
  • The mutation(s) in cancer cells and the change to the expression of those genes regulate how rapidly the tumor grows, its likelihood of metastasizing and moving throughout the body, whether or not its growth is supported by the hormones estrogen or progesterone, whether it over-expresses certain proteins such as HER2 , and how responsive it will be to different treatments. (
  • Through gene expression, breast tumor genes regulate the timing and quantity of production of gene-specific RNA and proteins. (
  • Our primary interest is the genes that regulate how people respond to medicines, such as cancer chemotherapy," senior study author Eileen Dolan, a professor of medicine at the University of Chicago, said in a prepared statement. (
  • Interestingly, when valproic acid was administered, the neurons showed increased gene activation, including increased levels of LEF1 . (
  • The gene expression of individual neurons can be examined following patch clamp experiments by performing single cell PCR on the cytosol aspirated from the patched cell. (
  • Our results point to the fact that the gene expression pattern is one of the most reliable ways to map the cellular identity of individual neurons, since classification based on morpho-electrical properties fails to capture all the neuronal subtypes. (
  • 4. Gene expression of serotonergic neurons in the central nervous system: molecular and developmental analysis. (
  • For example, Olson and his colleagues realized that one fifth of the genes altered in Huntington's seem to be regulated by retinoids, molecules that modulate the development and maturation of neurons. (
  • Then the researchers sequenced RNA from these neurons to find out which genes were misregulated when compared to untreated neurons. (
  • A publicly available dataset containing gene expression data of 42 tumor tissues of the embryonal central nervous system (CNS) [1] is used for this example. (
  • From the expression data of all 42 samples in the dataset, extract the data of the 10 MD samples and the 10 Mglio samples. (
  • We tested our method on a collection of yeast expression profiles and on a human cancer dataset. (
  • We present algorithms for time-series gene expression analysis that permit the principled estimation of unobserved time points, clustering, and dataset alignment. (
  • Researchers in the laboratory of Mikhail Shapiro, assistant professor of chemical engineering and Heritage Medical Research Institute Investigator, have invented a new method to link magnetic resonance imaging (MRI) signals to gene expression in cells--including tumor cells--in living tissues. (
  • Previously developed reporter genes for MRI have been much more limited in their capabilities, requiring the use of specific metals that are not always available in some tissues. (
  • For each such gene, the investigators then compared expression of the two alleles head-to-head in 52 different tissues. (
  • Expression of Neanderthal alleles tended to be especially low in the brain and the testes, suggesting that those tissues may have experienced more rapid evolution since we diverged from Neanderthals approximately 700,000 years ago. (
  • Different tissues express different sets of genes based on their role in the body. (
  • We have attempted to detect cells committed to the melanocytic lineage, in human tissues, by means of tyrosinase gene expression. (
  • Therefore, it appears likely that cells expressing the tyrosinase gene are present in a wide range of human tissues. (
  • When and where genes are expressed (active) in tissues or cells is one of the main determinants of what makes that tissue or cell the way it is, both in terms of morphology and in terms of response to external stimuli. (
  • Several different methods exist for generating gene expression levels for all of the genes in the genome in tissues or even at cell-type-specific resolution. (
  • To determine the metabolism location of the extra-cellular matrix proteins in fugu (Takifugu rubripes), we cloned the cDNAs of the fugu gelatinases, matrix metalloproteinase-2 (MMP-2) and MMP-9, and examined their expressions in various adult tissues using a quantitative real-time PCR. (
  • The expression profiles of fugu gelatinases were different among tissues. (
  • Expression targeting involves the use of tissue‐specific enhancer/promoter elements to target the expression of therapeutic transgenes to tissues of interest. (
  • We'll also discuss the best-characterised epigenetic modification, DNA methylation, and how it is not only implicated in regulating gene expression, but also in maintaining genome stability. (
  • The definition of retina-specific promoters will make possible targeted expression of heterologous and altered genes to the retina. (
  • Nettelbeck DM, Jerome V and Muller R (2000) Gene therapy designer promoters for tumour targeting. (
  • A non-radioactive in situ hybridization assay will be used to look at the spatial and developmental pattern of expression of rhodopsin and other retinal genes in retinal whole mounts. (
  • By exploring evolutionary histories of gene regulation with particular reference to developmental and behavioural genes in humans, you will gain a comprehensive understanding of the role that evolution plays in the diversity and maintenance of different life forms. (
  • For example, a recent study found that forms of a gene associated with schizophrenia are over-expressed in the fetal brain, adding to evidence implicating this critical developmental period. (
  • This project will allow us to document which individual genes and sets of genes are turned on and off in different brain regions through the whole developmental time period," said Knowles, the principal investigator on the project. (
  • The human genome contains on the order of 25,000 genes which work in concert to produce on the order of 1,000,000 distinct proteins. (
  • While knowledge of the precise proteins a cell makes (proteomics) is more relevant than knowing how much messenger RNA is made from each gene, gene expression profiling provides the most global picture possible in a single experiment. (
  • Gene expression is the process by which DNA is used to make proteins, which then go on to perform various important functions in the body. (
  • Further, the genes themselves did not control the levels of their own expression, rather noncoding regions adjacent to them determined whether to ratchet up or down the proteins or other functional end products the genes encoded. (
  • The phenotypic expression of these genes, through the synthesis of specific proteins, involves interaction with environmental signals that trigger activation of particular genes. (
  • Gene expression is the process of making specific proteins from the information contained in the genes . (
  • Gene expression tests evaluate the RNA in a person's tissue sample to determine which genes are actively making proteins. (
  • These are tests that evaluate the products (RNA) of specific groups of genes in malignant tumor tissue from the breast in order to determine which genes are making proteins in the tumor tissue. (
  • Postdoctoral Research Associate: Isolation and characterization of sperm-expressed genes and proteins from Zea mays Despite the crucial role of sperm in fertilization, virtually nothing is known about sperm cell gene expression, largely because sperm cells in the quantities necessary for molecular and biochemical characterization could not be obtained, and because partially purified sperm cells are often highly contaminated with the cytoplasm of the vegetative cell. (
  • Once sperm-surface proteins are identified, homologs of these genes will be obtained via PCR and/or database searches of other plant genomes. (
  • Gene expression is an active ongoing process that maintains a functional CNS, as proteins are being made on a continual basis. (
  • A regulatory gene , located before the operon, continually makes repressor proteins that bind with the operator and prohibit the function of RNA polymerase. (
  • A new survey of mammalian RNA revealed genome-wide variations in gene expression between male and female mammals. (
  • After analyzing some of the nearly 1,100 genes in detail, Cheung and Spielman believe that the expression level discrepancies were due to nucleotide differences in noncoding regions around the genes, and not the genes themselves. (
  • Sex differences in muscle gene expression were further reduced with long-term endurance exercise, an international research team found. (
  • We can infer that maybe the greatest differences in gene regulation exist in the brain and testes between modern humans and Neanderthals," says Akey. (
  • For this study, McCoy and his colleagues had to develop a new statistical approach to sift through the immense amount of RNA data, but the same technique could be used to compare gene expression differences between modern human alleles. (
  • The study to be published on March 22 in PLoS Genetics uncovered differences in gene expression between brain tissue from young (2 to14 years old) and adult individuals with autism syndrome disorder, providing important clues why brain growth and development is abnormal in this disorder. (
  • THURSDAY, Feb. 28 -- People of European and African ancestry have differences in gene expression levels that affect how they respond to certain kinds of drugs and fight off specific types of infections, says a new U.S. study. (
  • But they also made some unexpected findings of major differences in expression levels of genes involved in a communication system that governs basic cellular activities and coordinates cell actions. (
  • Population differences in gene expression have only recently begun to be investigated. (
  • By performing single-cell analysis on around 8000 cells taken from 48 post-mortem human brains, half with distinctive AD pathology and half with no signs of the disease, the researchers were able to analyze all cell types and differentiate between them, showing the distinct gene expression differences that can occur in the disease [7]. (
  • Gene expression differences in two groups of samples are discovered and quantificated by comparison of signal intensity values in microarray spots. (
  • July 19 (UPI) -- Most of the science looking at gender differences among mammals have focused on physiological differences, but new research suggests many of those more outward-facing differences -- like height and facial hair -- are rooted in differences in the way males and females express their genes. (
  • Discovering contributions of sex biased gene expression to height is exciting because identifying the determinants of height is a classic, century-old problem, and yet by looking at sex differences in this new way we were able to provide new insights," David Page, director of the Whitehead Institute at MIT, said in a news release . (
  • We're not saying to avoid animal models in sex differences research, only not to take for granted that the sex biased gene expression behind a trait or disease observed in an animal will be the same as that in humans," said Sahin Naqvi, former MIT graduate student, now a postdoctoral researcher at Stanford. (
  • April 11, 2000] A communiqué from Michael Hoffman of Rosetta Inpharmatics reports on the development of GEML - an open-standard XML format for DNA microarray and gene expression data. (
  • November 29, 2000] "On behalf of the GEML Community, Rosetta Inpharmatics has submitted to the Object Management Group (OMG) a proposed DTD based on the new version of Gene Expression Markup Language - GEML 2.0. (
  • November 29, 2000] Rosetta Inpharmatics' Initial Submission regarding the OMG's Gene Expression RFP -- XML DTD for GEML 2.0. (
  • A study from 2012 showed that in the brain's microvessels alone, there can be up to 2000 genes with altered expression in the brains of AD patients. (
  • Steinwaerder D and Lieber A (2000) Insulation from viral transcriptional regulatory elements improves inducible transgene expression from adenovirus vectors in vitro and in vivo. (
  • Stone D, David A, Bolognani F, Lowenstein PR and Castro MG (2000) Viral vectors for gene delivery and gene therapy within the endocrine system. (
  • In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. (
  • There is a critical look at gene therapy and other future therapeutic strategies based on the molecular and cellular causes of atherosclerosis. (
  • An effective reporter gene for MRI is a 'holy grail' in biomedical imaging because it would allow cellular function to be observed non-invasively," says Shapiro. (
  • The paper is titled "Non-invasive imaging using reporter genes altering cellular water permeability. (
  • From the expression profiles of all the neuron subtypes, we aim to identify the minimal set of genes whose cellular expression pattern uniquely defines the neuron subtype. (
  • Our TaqMan® Assays are primer and probe sets based on 5' nuclease chemistry using TaqMan® MGB (minor groove binder) probes, the most quantitative gene expression technology available. (
  • Along with graduate student Yun Zhang and professor of veterinary medicine Gregory J. Phillips, Yeung used the chemiluminescence technique to perform quantitative studies of gene expression in two bacterial lines. (
  • The two-year Grand Opportunity grant, funded through the National Institutes of Mental Health, will allow researchers to use DNA sequencing and profiling technologies to create an atlas of when and where thousands of genes are expressed during key periods of development. (
  • The team used gene chips, which reveal the activities of thousands of genes at once, to monitor approximately 6,000 genes in the striatum, a brain region particularly damaged by Huntington's. (
  • A Relative Expression Analysis (RXA) uses ordering relationships in a small collection of genes and is successfully applied to classiffication using microarray data. (
  • Expression profiling is a logical next step after sequencing a genome: the sequence tells us what the cell could possibly do, while the expression profile tells us what it is actually doing at a point in time. (
  • 7. A method according to claim 3, wherein the sequence that causes male sterility is the RIP gene linked to an anther specific promoter. (
  • The postdoc will prepare cDNA libraries from highly purified maize sperm (available via our collaboration with the RDP, Lyon, France) and sequence large numbers of clones, in order to obtain a representation of sperm -expressed genes. (
  • Examination of sequence diversity demonstrated a significant positive association with diversity in gene expression. (
  • An alga that seems healthy from a physiological point of view appears altered when its gene's expression is examined. (
  • In each mouse line, expression of the reporter or recombinase is controlled by promoter elements derived from a bacterial artificial chromosome (BAC) containing a specific gene of interest, in order to mimic the gene's expression pattern. (
  • Dr. Josh Dubnau introduces a technique for examining gene expression in the brains of fruit flies. (
  • Until now, few studies have been able to investigate whole-genome gene expression and genotype variation in the brains of young patients with autism, especially in regions such as the prefrontal cortex that display the greatest growth abnormality. (
  • Past studies have investigated the gene expression profile of the brain of an Alzheimer's patient, with each additional study increasing understanding of how the affected brains differ. (
  • One of these groups of chemicals altered the levels of many of the same genes that are altered in the brains of people with autism or Alzheimer's disease. (
  • 1997) Efficient muscle‐specific transgene expression after adenovirus‐mediated gene transfer in mice using a 1.35 kb muscle creatine kinase promoter/enhancer. (
  • These studies identified variation throughout the genome, including single-nucleotide and INDEL polymorphisms in all gene components: promoter, UTRs, ORFs, and introns. (
  • Moreover, use of the ordering relationships for a small collection of genes has potential for identification of gene-gene interactions with plausible biological interpretation and direct clinical applicability [ 4 ]. (
  • The researchers were able to analyse the transcriptome of the microalgae -- i.e. the set of all RNA molecules that controls the expression of their genes -- using molecular biology tools. (
  • Researchers developed a highly multiplexed screen to quantify the co-expression of 351 molecules on the surfaces of millions of human B cells. (
  • We thought that if we could link signals from water molecules to the expression of genes of interest, we could change the way the cell looks under MRI," says Arnab Mukherjee, a postdoctoral scholar in chemical engineering at Caltech and co-lead author on the paper. (
  • The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of nonprotein molecules. (
  • Microarray gene expression analysis is high-throughput method in which many different sized DNA molecules are attached to solid surface in designated spots. (
  • Due to popular demand, we are making the subset of 384 genes we used in Ka Yee's dissertation available (as text-delimited file). (
  • They found that a small subset of genes, less than two percent of the total, was affected by the disease. (
  • Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. (
  • The complex nature of mitochondrial gene expression that relies on two different genomes calls for a multidisciplinary approach in which different teams of researchers join forces. (
  • Here, we…observed that the Wnt/ß-catenin signaling pathway is profoundly affected, with a significant decrease in expression of LEF1 ," the researchers write. (
  • Mutations in key genes may result in changes to how a step proceeds (or doesn't) and may cause a change to the end product or any intermediate product along the pathway. (
  • Systemic analysis of data gathered in microarray gene expression measurement is performed by various bioinformatic methods such as group analysis, annotation analysis as well as network and pathway analysis. (
  • His current research interests include rate control and noise in the eukaryotic gene expression pathway, and a range of synthetic biology projects. (
  • A team from King's College London, UK, looked at so-called "epigenetic changes" involved in ASD, in which the environment affects the expression of genes without changing the underlying DNA. (
  • Because identical twins share the same genes, the fact that ASD can occur in one twin and not the other suggests that epigenetic factors may be involved. (
  • An introduction to and definition of epigenetic control of gene expression, and its importance in normal development. (
  • Many genes are also subjected to age-dependent transcriptional regulation. (
  • We study fundamental aspects of gene regulation, from transcriptional initiation to histone modification and chromatin remodelling. (
  • 1997) Transcriptional targeting of replication‐defective adenovirus transgene expression to smooth muscle cells in vivo. (
  • Clary BM and Lyerly HK (1998) Transcriptional targeting for cancer gene therapy. (
  • Spear MA (1998) Gene therapy of gliomas: receptor and transcriptional targeting. (
  • The researchers found that changes in histone modification at HPV integration events were correlated with upregulation of nearby genes and endogenous retroviruses. (
  • GeneLab undertakes specific research and training projects, which require expertise in gene cloning, PCR, DNA sequencing, cDNA library construction, gene expression and other molecular methods. (
  • Now researchers from North Carolina State University have found a way to use naturally occurring hydrogen peroxide inside cells to switch on gene expression. (
  • Researchers have been conducting studies such as the International HapMap Project--a global effort to catalogue common single-nucleotide variations, such as the addition, deletion or substitution of a base in the code of a gene--to get to the bottom of long-observed correlations between ethnicity and common complex diseases. (
  • When the researchers then compared the Asian populations with the Caucasian sampling, they noted that 1,097, more than 25 percent, of the genes had differing expression levels. (
  • Researchers have developed a method, called MemorySeq, to identify genes transcriptome-wide whose expression fluctuations persist for several cell divisions. (
  • A team of researchers in Germany investigated gene expression in airway cells of SARS-CoV-2 patients, finding increased interaction between epithelial and immune cells. (
  • The researchers showed that this technique was successful in monitoring gene expression in a brain tumor in mice. (
  • Neanderthal genes' effects on gene expression likely contribute to traits such as height and susceptibility to schizophrenia or lupus, the researchers found. (
  • For their newly reported study, the researchers initially conducted a systematic review of published gene signatures identified in blood samples from people with TB, compared with healthy individuals. (
  • Researchers studied 30 white families from Utah and 30 Yoruban families from Nigeria, and found significant variations in nearly 5 percent of the 9,156 genes they analyzed. (
  • In support of this, the researchers found that there is a significant change in the genes associated with axon regeneration and myelination [9]. (
  • Researchers determined that sex-biased gene expression accounts for 12 percent of the difference in average height between males and females. (
  • Ultimately, the researchers found that commonly used chemicals produced gene expression changes similar to those in people with autism and neurodegenerative conditions, including Alzheimer's disease and Huntington's disease. (
  • In the course of their work, the researchers created hundreds of data sets of gene expression. (
  • Cross validation results show high specificity in assigning function to genes based on their biclusters, and we are able to annotate in this way 196 uncharacterized yeast genes. (
  • We demonstrate that our algorithm produces stable low-error alignments on real expression data and further show a specific application to yeast knock-out data that produces biologically meaningful results. (
  • 1996) Expression monitoring by hybridization to high‐density oligonucleotide arrays. (
  • Their results, reported in this week's Nature Genetics, were that different ethnic groups not only carried different genes, but there were greater disparities than previously believed in the degrees to which genes that were the same among ethnic groups were expressed. (
  • Now, a Swedish study published in Human Molecular Genetics shows that drinking tea can affect gene expression in women. (
  • Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. (
  • The technique, which eventually could be used in humans, would allow gene expression to be monitored non-invasively, requiring no surgical procedures such as biopsies. (
  • They can, however, look to gene expression in modern humans who possess Neanderthal ancestry. (
  • Next steps may include investigating whether Denisovans--another species of hominins that crossbred with modern humans--are contributing to gene expression, as well as applying the side-by-side method of expression analysis more broadly. (
  • The sex-biased genes identified in humans were not always the same genes found to be sex-biased in different animal species. (
  • The genome of gene expression programming consists of a linear, symbolic string or chromosome of fixed length composed of one or more genes of equal size. (
  • An example of a chromosome with two genes, each of size 9, is the string (position zero indicates the start of each gene): 012345678012345678 L+a-baccd**cLabacd where "L" represents the natural logarithm function and "a", "b", "c", and "d" represent the variables and constants used in a problem. (
  • One gene has been located on chromosome 8. (
  • They designed a molecule that was sensitive to hydrogen peroxide and enabled expression of the firefly luciferase gene in live mammalian cells. (
  • A decrease in activation of a specific gene may explain why many patients with bipolar disorder (BD) do not respond to lithium , new research suggests. (
  • This new volume on gene expression and epigenetics discusses environmental effects related to specific gene expression. (
  • Rothmann T, Katus HA, Hartong R, Perricaudet M and Franz WM (1996) Heart muscle‐specific gene expression using replicative defective recombinant adenovirus. (
  • The figure above represents the output of a two dimensional cluster, in which similar samples (rows, above) and similar gene probes (columns) were organized so that they would lie close together. (
  • Many factors determine whether a gene is on or off, such as the time of day, whether or not the cell is actively dividing, its local environment, and chemical signals from other cells. (
  • The exciting aspects of this synthetic gene switch are that you can use it not only as a way to detect the presence of hydrogen peroxide within cells, in this case, by making cells containing hydrogen peroxide glow, but also to express any gene of interest in response to the presence of hydrogen peroxide," Deiters says. (
  • J:64018 Wang CC, Biben C, Robb L, Nassir F, Barnett L, Davidson NO, Koentgen F, Tarlinton D, Harvey RP, Homeodomain factor Nkx2-3 controls regional expression of leukocyte homing coreceptor MAdCAM-1 in specialized endothelial cells of the viscera. (
  • From its microarray, the team measured 4,197 genes expressed by cells. (
  • Genes tell cells what to do -- for example, when to repair DNA mistakes or when to die--and can be turned on or off like a light switch. (
  • Now, for the first time, Caltech scientists have developed a simple way to visualize gene expression in cells deep inside the body using a common imaging technology. (
  • After implanting the tumor, they gave the mice a drug to trigger the tumor cells to express the aquaporin reporter gene, which made the tumor look darker in MRI images. (
  • This paper by Karin Roesch , Michael B. Stadler and Constance L. Cepko looks at gene expression changes in the Müller cells, one of the glial cells of the retina as the rd1 mouse retina degenerates. (
  • Approximately 3 to 5 percent of genes are active in a particular cell, even though all cells have the same information contained in their DNA. (
  • - In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. (
  • The genes that control the number of brain cells did not have the normal functional expression, and the level of gene expression that governs the pattern of neural organization across the prefrontal cortex is turned down. (
  • Loss of ACL in cultured mouse 3T3-L1 cells diminished the increase in histone acetylation normally associated with hormone-stimulated differentiation of these cells and inhibited the increase in expression of specific genes, such as that encoding the Glut4 glucose transporter. (
  • Because gene expression within a cell population fluctuates randomly, investigators must monitor individual cells if they want to follow the activity as genes switch on and off. (
  • A Relative Expression Analysis (RXA) was firstly proposed by Geman et al. (
  • The Afirma Gene Expression Classifier is offered as part of Veracyte's comprehensive Afirma Thyroid FNA Analysis, which combines specialized cytopathology assessment for initial review of thyroid nodule FNAs, with the gene expression test used to clarify inconclusive results. (
  • These databases allow one to access, select, retrieve and combine for analysis gene expression datasets generated by one's own or other laboratories. (
  • Velculescu VE, Zhang L, Vogelstein B and Kinzler KW (1995) Serial analysis of gene expression. (
  • Selected genes will be targeted for further analysis. (
  • Section I - Techniques in Analysis of Gene Expression. (
  • 1. The gene knockout technology for the analysis of learning and memory, and neural development. (
  • A gene expression classifier raised suspicion of Medullary Thyroid Cancer (MTC) in pre-operative fine needle aspiration (FNA) analysis of cytologically indeterminate and malignant neoplasms. (
  • Expression analysis data is generated by a variety of sources and is usually stored in the form of disk files. (
  • Rosetta has been continuously loading XML files on the order of thirteen megabytes into the Rosetta Resolver system, an enterprise expression data analysis product. (
  • proposals which define interfaces and services in support of array based gene expression data collection, management, retrieval, and analysis. (
  • Prostate tumor samples were taken from the patients at the time of surgery and subjected to a microarray gene expression analysis as described in Methods. (
  • SNY ), today announced that the New York State Department of Health has issued a license enabling Veracyte's Afirma® Gene Expression Classifier to be offered to patients in the state. (
  • We are delighted that the Afirma Gene Expression Classifier will now be available to physicians and their patients in New York State and that Memorial Sloan-Kettering is among the first in the state to offer our test,' said Bonnie Anderson , Veracyte's cofounder and chief executive officer. (
  • The Afirma Gene Expression Classifier measures the expression of 142 genes to reclassify ambiguous thyroid FNA samples as either benign or suspicious for cancer. (
  • The gene expression classifier was run prospectively and pre-operatively on all cytologically indeterminant samples. (
  • With the development of in vivo delivery vectors, these selective expression cassettes can now be used for preclinical assessment of their therapeutic potential for the treatment of inherited diseases or cancers. (
  • You can now compare the gene expression values between two groups of data: CNS medulloblastomas (MD) and non-neuronal origin malignant gliomas (Mglio) tumor. (
  • LEF1 commonly pairs with beta-catenin, which typically leads to the activation of other genes for regulating neuronal activity levels. (
  • We have successfully used this approach to correlate the expression of more than 30 genes coding for ion channels and neuronal markers with the electropysiological and morphological properties [1-4]. (
  • Section II - Signal Transduction and Gene Expression. (
  • Although some of the mice in this study were just beginning to show symptoms, they had already gone through many neurological alterations, so many of the newfound gene changes probably reflect secondary events, including the brain's attempts at compensating for earlier damage. (
  • We propose a new method to detect significant biclusters in large expression datasets. (
  • Experimental validation of EvoTSP on public available microarray datasets showed that the proposed solution significantly outperforms in terms of accuracy other relative expression algorithms and allows exploring much larger solution space. (
  • And those variations in gene expression contribute to human phenotypic variation and disease susceptibility. (