A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
A former branch of knowledge embracing the study, description, and classification of natural objects (as animals, plants, and minerals) and thus including the modern sciences of zoology, botany, and mineralogy insofar as they existed at that time. In the 17th, 18th, and 19th centuries it was much used for the generalized pursuit of certain areas of science. (Webster, 3d ed; from Dr. James H. Cassedy, NLM History of Medicine Division)
Annelids of the class Hirudinea. Some species, the bloodsuckers, may become temporarily parasitic upon animals, including man. Medicinal leeches (HIRUDO MEDICINALIS) have been used therapeutically for drawing blood since ancient times.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.
A surgical specialty concerned with the study and treatment of disorders of the ear, nose, and throat.
The application of genetic analyses and MOLECULAR DIAGNOSTIC TECHNIQUES to legal matters and crime analysis.
A group of islands in the southwest Pacific. Its capital is Wellington. It was discovered by the Dutch explorer Abel Tasman in 1642 and circumnavigated by Cook in 1769. Colonized in 1840 by the New Zealand Company, it became a British crown colony in 1840 until 1907 when colonial status was terminated. New Zealand is a partly anglicized form of the original Dutch name Nieuw Zeeland, new sea land, possibly with reference to the Dutch province of Zeeland. (From Webster's New Geographical Dictionary, 1988, p842 & Room, Brewer's Dictionary of Names, 1992, p378)
Vaccines used in conjunction with diagnostic tests to differentiate vaccinated animals from carrier animals. Marker vaccines can be either a subunit or a gene-deleted vaccine.
The inactive form of GLYCOGEN PHOSPHORYLASE that is converted to the active form PHOSPHORYLASE A via phosphorylation by PHOSPHORYLASE KINASE and ATP.
The sum of the weight of all the atoms in a molecule.
A class of glucosyltransferases that catalyzes the degradation of storage polysaccharides, such as glucose polymers, by phosphorolysis in animals (GLYCOGEN PHOSPHORYLASE) and in plants (STARCH PHOSPHORYLASE).
The larger subunits of MYOSINS. The heavy chains have a molecular weight of about 230 kDa and each heavy chain is usually associated with a dissimilar pair of MYOSIN LIGHT CHAINS. The heavy chains possess actin-binding and ATPase activity.
A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
A water-soluble aminoglycosidic antibiotic complex isolated from fermentation filtrates of Bacillus circulans. Two components (A and B) have been separated from the complex. Both are active against many gram-positive and some gram-negative bacteria.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.

Partial purification and properties of porcine thymus lactosylceramide beta-galactosidase. (1/1063)

Porcine thymus lactosylceramide beta-galactosidase was purified by a simple procedure. In the final step of isoelectric focusing the enzyme was separated into two peaks of pI 6.3 (peak I) and 7.0 (peak II), which showed 3,600- and 4,000-fold enhancement of lactosylceramide-hydrolysing activity, respectively. The two peaks had identical mobility on polyacrylamide gel electrophoresis. The apparent molecular weight was 34,000. Neither monosialoganglioside (GM1) nor galactosylceramide was hydrolysed by the purified enzyme fractions. The optimal pH was at 4.6, and sodium taurocholate was essential for the reaction. The apparent Km was 2.3 x 10-5 M. The reaction was stimulated by sodium chloride and linoleic acid, while it was strongly inhibited by Triton X-100 and bovine serum albumin. Galactosylceramide, p-nitrophenyl beta-galactoside, and p-nitrophenol were weak inhibitors. No effects of GM1 and galactose were observed on the hydrolysis of lactosylceramide.  (+info)

A quantitative study of pinocytosis and lysosome function in experimentally induced lysosomal storage. (2/1063)

The highly pinocytic epithelial cells of the visceral yolk sac from 17.5-day rat conceptuses were used as a model in which to induce engorgement of the vacuolar system by direct accumulation of substances that are not hydrolysed by lysosomal enzymes. The ultra-structural appearances of these cells in pregnant animals that 24-48h before had received intraperitoneal injections of Triton WR-1339, polyvinylpyrrolidone, dextran or sucrose revealed gross abnormalities that were confined to the vacuolar system; in comparison with normal tissue the number, and in some cases the size, of vacuoles was increased, leading to close packing within the apical cytoplasm and distortion of the normal rounded shape. By culturing yolk sacs in vitro, rates of ingestion of 125I-labelled polyvinylpyrrolidone and of 125I-labelled bovine serum albumin were determined, together with the rate of digestion of the labelled protein. The rates of exocytosis of 125I-labelled polyvinylpyrrolidone and of lysosomal enzymes were also determined. No significant differences between normal and highly vacuolated tissues were found. Apparently marked vacuolation of these cells by these agents is without significant effect on pinocytosis, exocytosis or intralysosomal proteolysis.  (+info)

Latency of some glycosidases of rat liver lysosomes. (3/1063)

The latency of the alpha-glucosidase activity of intact rat liver lysosomes was studied by using four substrates (glycogen, maltose, p-nitrophenyl, alpha-glucoside, alpha-fluoroglucoside) at a range of substrate concentrations. The results indicate that the entire lysosome population is impermeable to glycogen and maltose, but a proportion of lysosomes are permeable to alpha-fluoroglucoside and a still higher proportion permeable to p-nitrophenyl alpha-glucoside. Incubation at 37 degrees C in an osmotically protected buffer of of pH 5.0 caused lysosomes to become permeable to previously impermeant substrates and ultimately to release their alpha-glucosidase into the medium. The latencies of lysosomal beta-glucosidase and beta-galactosidase were examined by using p-nitrophenyl beta-glucoside and beta-galactoside as substrates. The results indicate permeability properties to these substrates similar to that to p-nitrophenyl alpha-glucoside. On incubation in an osmotically protected buffer of pH 5, lysosomes progressively released their beta-galactosidase in soluble form, but beta-glucosidase remained attached to sedimentable material. Lysosomal beta-glucosidase was inhibited by 0.1% Triton X-100; alpha-glucosidase and beta-galactosidase were not inhibited.  (+info)

Cooperation of Sp1 and p300 in the induction of the CDK inhibitor p21WAF1/CIP1 during NGF-mediated neuronal differentiation. (4/1063)

Addition of nerve growth factor (NGF) to PC12 cells promotes neuronal differentiation while inhibiting cell proliferation. In order to understand how NGF exerts its antimitogenic effect during differentiation, we have studied the mechanism by which this factor activates the promoter of the CDK inhibitor p21W4F1/CIP1. The minimal region of the p21 promoter required for the NGF-induction was mapped to a contiguous stretch of 10 bp located 83 bases upstream of the transcription initiation site. This GC-rich region was shown to interact specifically with the transcription factor Sp1 and the related protein Sp3, in either exponentially-growing or NGF-treated PC12 cells. The addition of NGF resulted in an accumulation of the transcriptional co-activator p300 in complexes associated with the NGF-responsive region. Transcriptional activity of Sp1, Sp3 and p300 was specifically induced by NGF in a Gal4-fusion assay, indicating that induction of p21 during neuronal differentiation may involve regulation of the activity of these factors by NGF. Furthermore, p300 was able to act as a co-activator for Sp1-mediated transcriptional activation in PC12 cells, suggesting that p300 and Sp1 may cooperate in activating p21 transcription during the withdrawal of neuronal precursors from the cell cycle. This hypothesis is supported by experiments showing that p300 and Sp1 form complexes in PC12 cells.  (+info)

Structural equivalents of latency for lysosome hydrolases. (5/1063)

1. Structure-linked latency, a trait for most lysosome hydrolase activities, is customarily ascribed to the permeability-barrier function performed by the particle-limiting membrane, which shields enzyme sites from externally added substrates. 2. The influence of various substrate concentrations on the reaction rate has been measured for both free (non-latent) and total (completely unmasked by Triton X-100) hydrolase activities in rat liver cell-free preparations. The substrates were: beta-glycerophosphate, phenolphthalein mono-beta-glucuronide. p-nitrophenyl N-acetyl-beta-D-glucosaminide and p-nitrophenyl beta-D-galactopyranoside. The ratio (free activity/total activity) X 100 is called fractional free activity at any given substrate concentration. 3. The fractional free activity of beta-glucuronidase and beta-N-acetylglucosaminidase were clearly independent of substrate concentration, over the range examined, in both homogenates and lysosome-rich fractions. The fractional free activity of acid phosphatase appeared to be either unaffected (homogenate) or even depressed (lysosome-rich fraction) by increasing the beta-glycerophosphate concentration. The fractional free activity of beta-galactosidase consistently showed a non-linear increase with increasing substrate concentration in both homogenates and lysosome-rich fractions. 4. Procedures such as treatment with digitonin, hypo-osmotic shock and acid autolysis, although effective in causing varying degrees of resolution of the latency of lysosome hydrolase activities, were unable to modify appreciably the pattern of dependence or independence of their fractional free activities on substrate concentration, as compared with that exhibited by control preparations. Ouabain did not affect the free beta-N-acetylglucosaminidase activity of liver homogenates at all. 5. Preincubation of control preparations with beta-glycerophosphate or p-nitrophenyl beta-galactoside did not result in any significant stimulation of the free hydrolytic activity toward these substrates. 6. The results consistently support the view that the membrane of "intact" lysosomes is virtually impermeable to all the substrates tested, except for p-nitrophenyl beta-galactoside, for which the evidence is contradictory. Moreover the progressive unmasking of the hydrolase activities produced by these procedures in vitro reflects the increasing proportion of enzyme sites that are fully accessible to their substrates rather than a graded increase in the permeability of the lysosomal membrane.  (+info)

beta-Galactosidase is induced by hormone in Drosophila melanogaster cell cultures. (6/1063)

Drosophila melanogaster cell lines Kc and Ca and clones FC and RF6, cultured in vitro, have no detectable beta-galactosidase (beta-galactoside galactohydrolase, EC 3.2.1.23) activity (as measured by hydrolysis of o-nitrophenyl-beta-D-galoctoside). Ecdysterone, a hormonal steroid of critical importance in insect physiology, clearly induces beta-galactosidase activity in D. melanogaster cells cultured in vitro. Induction occurs in cell lines or clones known to be sensitive to ecdysterone (K, Ca, and Fc) and does not occur in clones known to be resistant to the hormone (RF6). Some properties of the hormone-induced beta-galactosidase activity were studied. The Km for o-nitrophenyl galactoside is 0.35 mM and the Ki for lactose is 12 mM (similar to those of Escherichia coli beta-galactosidase); the activity can be recovered after sodium dodecyl sulfate treatment; the enzyme is a tetramer (Mr of the monomer is 64,000).  (+info)

Characterization of purified human liver acid beta-D-galactosidases A2 and A3. (7/1063)

1. Human liver acid beta-galactosidase A2 and A3 were isolated by chromatography on concanavalin A-Sepharose 4B, Sepharose 6B, and Sepharose 4B-6-aminohexyl 1-thio-beta-D-galactopyranoside. beta-Galactosidase A2 and A3 were purified to final specific activities of 45.5 and 20.6 mumol/min per mg respectively with 4-methylumbelliferyl beta-D-galactopyranoside as substrate. 2. Form A2 had a mol.wt. of 150000 +/- 15000 (gel filtration) and appeared as a single band of protein (mol.wt 65000 +/- 1000) on electrophoresis in the presence of sodium dodecyl sulphate. 3. Form A3 had a mol.wt. (gel filtration) of 660000 +/- 66000. On electrophoresis in the presence of sodium dodecyl sulphate, form A3 appeared as a major band of protein (72% of total) of mol.wt. 65000 +/- 1000 and minor protein bands of mol.wt. 44000 +/- 1000 and 26,000 +/- 1000 and 22000 +/- 1000. 4. Gel-filtration chromatography of purified beta-galactosidase A3 generated approximately equal amounts of forms A3 and A2. beta-Galactosidase A1 was not detected by gel-filtration chromatography of partially or highly purified preparations of forms A2 and A3. 5. Both forms A2 and A3 had identical isoelectric points of 4.42 +/- 0.02. The data suggest that forms A2 and A3 are dimeric and multimeric forms of beta-galactosidase A1. 6. Amino acid analysis of beta-galactosidase A2 gave a ratio of acidic to basic amino acids of 2.6:1. 7. beta-Galactosidase A2 contained 7.5% carbohydrate by weight and sialic acid, D-galactose, D-glucosamine and D-mannose were present in the molar proportions 1.1:1.0:1.7:2.7.  (+info)

Isolation and characterization of an endogenous inhibitor of protein synthesis in Escherichia coli K-12. (8/1063)

A low-molecular-weight factor was isolated from cell extracts of Escherichia coli K-12. The concentration of the factor in cells was dependent upon nutritional conditions, the concentration being higher in faster growing cells. Treatment of cells with colicin K caused an increase in concentration of the factor. The factor inhibited protein synthesis in E. coli. This inhibition was reversible, apparently because of metabolism of the factor. The inhibition of synthesis of beta-galactosidase lasted longer than the inhibition of protein synthesis; cyclic AMP eliminated this difference. The factor inhibited the synthesis of beta-galactosidase from preformed lac mRNA, indicating an inhibition of translation. Kinetic studies of the onset of inhibition of beta-galactosidase synthesis by the factor suggested that the factor may inhibit protein synthesis at the initiation of translation.  (+info)

Explanation of A & R The function of (3-galactosidase enzyme in lactose metabolism is to form glucose by cleaving lactose. Thus if both glucose and lactose are present in the growth medium, activity of lac operon is not needed, and indeed, no p-galactosidase is formed until virtually all of the glucose in the culture medium is consumed. The lack of synthesis of p-galactosidase is a result of lack of synthesis of lac mRNA. No lac mRNA is made in the presence of glucose, because in addition to an inducer for inactivating the lac i repressor, another element (cAMP-CAP) is needed for initiating lac mRNA synthesis. The activity of this element is regulated by the concentration of glucose ...
The results of this study are consistent with the following conclusions. First, unconjugated β-galactosidase is rapidly cleared from blood in vivo (Table 2), owing to rapid uptake of the unconjugated enzyme by liver and spleen (Figs. 2 and 3). Second, once inside cells, β-galactosidase is rapidly degraded in vivo such that 99% of the organ enzyme activity is lost at 4 h after an intravenous injection (Table 1). Third, the 116-kDa β-galactosidase (Fig. 1B) can be conjugated to the 8D3 TfRmAb without loss of enzyme activity (Fig. 1C). Fourth, there is minimal brain uptake of the unconjugated β-galactosidase, but there is a 10-fold increase in brain uptake of enzyme following conjugation to the 8D3 TfRmAb (Table 1; Figs. 2 and 3).. The β-galactosidase is rapidly removed from the blood due to the avid uptake of the enzyme by liver and spleen (Figs. 2 and 3), which confirms the earlier observation of Onodera et al. (1983). The blood concentration of the β-galactosidase-TfRmAb is 5- to 10-fold ...
β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. Substrates of different β-
Edvotek 300 kit shows how to clone a DNA fragment by using ligation, transformation and an assay of ß-galactosidase. For advanced students.
Page contains details about β-galactosidase/ZIF-8 MOF coating . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
본 웹사이트에 게시된 이메일 주소가 전자우편 수집 프로그램이나 그 밖의 기술적 장치를 이용하여 무단으로 수집되는 것을 거부하며, 이를 위반시 정보통신망법에 의해 형사처벌됨을 유념하시기 바랍니다. [게시일 2003년 4월 2일 ...
reacts with native and denatured-reduced E. coli β-galactosidase(116 kD); may be usedfor detection of β-galactosidase expressed by E. colilacZ gene encoded in many cloned gene sequences,and serves as an indicator for fusion proteins encodedby an inserted DNA ...
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Beta Galactosidase | Learn more about Beta Galactosidase | Meaning of Beta Galactosidase | Description of Beta Galactosidase | Details of Beta Galactosidase | Article on Beta Galactosidase | Essay on Beta Galactosidase | Definition of Beta Galactosidase | Infospaze
1. A method is described for following continuously the action of beta-galactosidase on 4-methylumbelliferyl beta-D-galactoside at pH 4.5, in which 4-methylumbelliferone production is measured at fluorescence excitation and emission wavelengths of 324 and 444nm respectively. 2. Initial-rate studies show that the presence of salt activates beta-galactosidase up to 100 mM, but is inhibitory above that concentration. The enzyme is very unstable at 37 degrees C and low ionic strength, but stability increases with ionic strength. 3. The stability of the enzyme at 37 degrees C decreases markedly with rising pH in the range 5.9-8.0. 4. Gel-filtration patterns demonstrate that there is a marked tendency to polymerization with increasing ionic strength. The gel-filtration pattern shows decreasing amounts of dimer with increasing pH. 5. The correlation between activity, stability and molecular form of beta-galactosidase is discussed. It is suggested that the dimeric form of the enzyme is the most stable ...
TY - JOUR. T1 - m-Fluorotyrosine substitution in β-galactosidase; Evidence for the existence of a catalytically active tyrosine. AU - Ring, M.. AU - Armitage, I. M.. AU - Huber, R. E.. N1 - Funding Information: This work was supported by the National Council (Canada) and the Alberta Heritage Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 1985/9/16. Y1 - 1985/9/16. N2 - The pH profiles of β-galactosidase, having tyr replaced by m-fluorotyrosine, were compared to those of normal enzyme. The inflection point on the alkaline side was lowered about 1.5 pH units in the fluoro-enzyme, corresponding to the difference in the phenolic pKa values of m-fluorotyrosine and tyr. When glycosidic bond breakage was rate-limiting, the Vm at pH 7.0 was higher for the fluoro-enzyme. When hydrolysis was rate-limiting or when acceptors which made transgalactosylis rate-limiting were used, the Vm was lower for the fluoro-enzyme. This shows that a tyr in β-galactosidase is a general-acid catalyst ...
Improving Soluble Expression of β-Galactosidase in Escherichia coli by Fusion with Thioredoxin - $\beta$-Galactosidase;Thioredoxin;Inclusion Body;IPTG;
Quantitation of β-galactosidase activity. In yeast cells, co-transformed with pGADT7 (AD) and pGBKT7 (BD) constructs as indicated, β-galactosidase activity wa
TY - JOUR. T1 - Purification and characterization of human liver β-galactosidase from a patient with the adult form of GM1 gangliosidosis and a normal control. AU - Muto, Tatsuro. AU - Naoi, Makoto. AU - Nagatsu, Toshiharu. AU - Takahashi, Akira. AU - Matsuoka, Yukihiko. AU - Hashizume, Yoshio. AU - Fujiki, Norio. PY - 1988/2/17. Y1 - 1988/2/17. N2 - β-Galactosidases were purified to homogeneity from livers of a normal control and a patient with the adult form of GM1 gangliosidosis. The purification was achieved by chromatography on DEAE-Sepharose fast flow, Con A-Sepharose, p-aminophenyl-1-thio-β-d-galactopyrarose, and QAE-Mono Q. The normal and mutant enzymes were purified about 5000-fold with a yield of 10% and 1800-fold with a yield of 34%, respectively, and could hydrolyze 4-methylumbelliferyl-β-d-galactoside, GM1 ganglioside, and asialofetuin. The purified normal enzyme was eluted from a TSK gel G-4000SW column as three symmetrical peaks of protein which were coincident with the three ...
β-galactosidase, also called beta-gal or β-gal, is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. SubstRates of different β-
β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this mutant, the ω-peptide, is unable to form a tetramer and is inactive. This mutant form of protein however may return fully to its active tetrameric state in the presence of an N-terminal fragment of the protein, the α-peptide. The rescue of function of the mutant β-galactosidase by the α-peptide is called α-complementation. In this method of screening, the host E. coli strain carries the lacZ deletion mutant (lacZΔM15) which contains the ω-peptide, while the plasmids used carry the lacZα sequence which encodes the first 59 residues of β-galactosidase, the α-peptide. Neither is functional by itself. However, when the two peptides are expressed together, as when a plasmid containing the lacZα sequence is transformed into a lacZΔM15 cells, ...
Product is fluorescein-5-isothiocyanate (FITC Isomer I) - comjugated goat IgG fraction to ß-galactosidase (no cross to E. coli) and buffer salts
This exercise was designed to provide an exciting introduction to specific gene structure and function. In the experiment, students are given two plasmids (A and B) which are identified in the instructors guide. One plasmid (A) has a functional gene for the enzyme ß-galactosidase. The ß-galactosidase gene in the other plasmid (B) is inactive because it contains a segment of foreign DNA. In the first part of the exercise, students analyze restriction digests of both plasmids in order to determine which plasmid should have a functional ß-galactosidase gene. In the second part of the exercise, the plasmids are introduced into E. coli by transformation and the color of the resulting colonies (blue or white) is then used to assess the functional status of the ß-galactosidase gene. This exercise can be completed within a single 3-hour laboratory session or two 2-hour laboratory periods.. ...
Page contains details about β-galactosidase expressing plasmid encapsulating liposomes . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Alpha-complementation of β-galactosidase does not seem to yield activities equal to wildtype β-galactosidase. Depending on the fragment, the activity can be up to 24% of wildtype [5]. (If anyone has a better reference comparing results from a Miller assay of alpha-complementated β-galactosidase with wildtype, please include it here.) ...
|strong|Rabbit anti beta-galactosidase polyclonal antibody|/strong| detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccha…
GF ID He_PIG_assoc #=GF AC PF10632.8 #=GF DE He_PIG associated, NEW1 domain of bacterial glycohydrolase #=GF AU Naumoff D, Coggill P #=GF SE Pfam-B_97991 (release 22.0) #=GF GA 25.00 25.00; #=GF TC 25.20 30.80; #=GF NC 22.50 23.40; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 26740544 -E 1000 --cpu 4 HMM pfamseq #=GF TP Domain #=GF RN [1] #=GF RM 15285616 #=GF RT [Phylogenetic analysis of alpha-galactosidases of the GH27 #=GF RT family]. #=GF RA Naumov DG; #=GF RL Mol Biol (Mosk). 2004;38:463-476. #=GF RN [2] #=GF RM 16131397 #=GF RT GH97 is a new family of glycoside hydrolases, which is related #=GF RT to the alpha-galactosidase superfamily. #=GF RA Naumoff DG; #=GF RL BMC Genomics. 2005;6:112. #=GF DR INTERPRO; IPR019599; #=GF CC The English-language version of the first reference can be found #=GF CC on pages 388-399 of the above. This domain has been named NEW1 #=GF CC but its actual function is not known. It is found on proteins #=GF CC which are bacterial galactosidases [1]. The ...
O-Nitrophenyl-β-D-Galactopyranoside (ONPG) Test. The objective of ONPG is to determine the ability of an organism to produce β-galactosidase.
Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN Anti Beta-Galactosidase (E. Coli) IgG-Biotin conjugate Antibody conjugate BGAL12-BTN
Beta-galactosidase molecule. Computer model showing the 4-chain-structure of bacterial beta-galactosidase. - Stock Image C035/6227
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Beta-galactosidase, molecular model. This enzyme breaks down sugars containing galactose, such as lactose, into their basic units (monosaccharides). - Stock Image C015/1970
Shop a large selection of Sample Prep Tools for Mass Spectrometry products and learn more about Alfa Aesar beta-Galactosidase Digest Standard, Certified Mass Spec Grade Quantity:
ATCC ® 87590™ Designation: YEplac112 TypeStrain=False Application: YE-type (episomal) shuttle vector vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Background: Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-D-galactoside galactohydrolase, EC 3.2.1.23), trivial called lactase. Orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial enzymes. ...
Substâncias complexas são convertidas, pela ação de enzimas, em moléculas solúveis, durante o processo de germinação, as quais são translocadas para a plântula em crescimento, servindo como fonte de energia ou estrutura física. Com o objetivo de quantificar a atividade enzimática da -galactosidase e a mobilização de mono e oligossacarídeos durante o período de germinação, foi conduzido este estudo utilizando-se sementes de jacarandá-da-bahia. As sementes foram mantidas em germinador a 25oC sob luz contínua, sendo avaliada a protrusão da radícula, pelo período de 10 dias. Foram quantificados os teores de mono e de oligossacarídeos, assim como a atividade específica da enzima -galactosidase nos tempos zero, um, três e cinco dias. Houve mobilização das reservas de glicose e manose nos cotilédones e xilose neste e no embrião nos três primeiros dias de germinação. A ramnose teve os teores aumentados nos cotilédones e no eixo embrionário. A rafinose foi o ...
Shop a large selection of Modifying Enzyemes products and learn more about MP Biomedicals - Galactosidase from Escherichia coli 5KU:Life Sciences 5KU.
Buy our Recombinant |em|E. coli |/em| beta Galactosidase protein. Ab79449 is a full length protein produced in Escherichia coli. Abcam provides free protocols…
TY - JOUR. T1 - Crystal structure of β-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants (共著). AU - Nakabayashi, Makoto. PY - 2015. Y1 - 2015. M3 - Article. JO - FEBS J. 2015 Jul;282(13):2540-52.. JF - FEBS J. 2015 Jul;282(13):2540-52.. ER - ...
This unit describes fixation and staining for b‐galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants
BioTek Notes dapplication, 26-Feb-07, Kinetic Analysis of ß-Galactosidase Activity using PowerWave™ HT Microplate Spectrophotometer and Gen5™ Data Analysis Software
The Lens serves almost all the patents and scholarly work in the world as a free, open and secure digital public good, with user privacy a paramount focus.
xyloglucan:xyloglucosyltransferase (EC 2.4.1.207); keratan-sulfate endo-1,4-β-galactosidase (EC 3.2.1.103); endo-1,3-β-glucanase (EC 3.2.1.39); endo-1,3(4)-β-glucanase (EC 3.2.1.6); licheninase (EC 3.2.1.73); β-agarase (EC 3.2.1.81); κ-carrageenase (EC 3.2.1.83); xyloglucanase (EC 3.2.1.151); endo-β-1,3-galactanase (EC 3.2.1.181); β-porphyranase (EC 3.2.1.178); hyaluronidase (EC 3.2.1.35); endo-β-1,4-galactosidase (EC 3.2.1.-); chitin β-1,6-glucanosyltransferase (EC 2.4.1.-); endo-β-1,4-galactosidase (EC 3.2.1.- ...
xyloglucan:xyloglucosyltransferase (EC 2.4.1.207); keratan-sulfate endo-1,4-β-galactosidase (EC 3.2.1.103); endo-1,3-β-glucanase (EC 3.2.1.39); endo-1,3(4)-β-glucanase (EC 3.2.1.6); licheninase (EC 3.2.1.73); β-agarase (EC 3.2.1.81); κ-carrageenase (EC 3.2.1.83); xyloglucanase (EC 3.2.1.151); endo-β-1,3-galactanase (EC 3.2.1.181); β-porphyranase (EC 3.2.1.178); hyaluronidase (EC 3.2.1.35); endo-β-1,4-galactosidase (EC 3.2.1.-); chitin β-1,6-glucanosyltransferase (EC 2.4.1.-); endo-β-1,4-galactosidase (EC 3.2.1.- ...
Compounds are evaluated for their binding to naturally occurring receptors, by employing the natural ligand conjugated to an enzyme donor fragment of β-galactosidase for competing with the sample compound for the natural acceptor binding site or in the absence of competition where the sample compound binds to an allosteric site. By adding the enzyme acceptor fragment of the β-galactosidase and substrate, the binding affinity of the sample compound may be evaluated as a measure of agonist or antagonist capability.
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An optimized chemiluminescent assay for beta-galactosidase using a chemiluminescent substrate AMPGD (3-(4-methoxyspiro[1,2-dioxetane-3,2-tricyclo-[3.3.1. 1(3,7)]decan]-4- yl)phenyl-beta-D-galactopyranoside) is described. This assay is rapid and sensitive and can detect as little as 2 fg of beta-gal …
ATCC ® 77267™ Designation: pUN65 TypeStrain=False Application: YC-type (centromeric) shuttle vector mutation detection shuttle vector vector permitting RNA synthesis in vitro vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
beta-Gal antibody [N2C3] (galactosidase, beta 1) for IHC-P, WB. Anti-beta-Gal pAb (GTX104360) is tested in Human, Rat samples. 100% Ab-Assurance.
Gene target information for GLB1L2 - galactosidase beta 1 like 2 (human). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Allcosmeticsource.com alpha galactosidase 2000u/g,5kg/bag,free shipping [EP170508006]- alpha galactosidase 2000u/g,1kg/bag,free shipping What is alpha galactosidase 2000u/g alpha galactosidase is an enzyme used to hydrolyze or break α-1, 6-glycosidic bonds into galactosyl oligosaccharides (α-galactosides), liberating simpler, more usable sugars and eliminating its anti-nutrient effect. Thus, it improves the utilization of the energy and proteins in feedstuff. Function of alpha galactosidase 2000u/g Hydrolyzing
microbiology lab days 5 and 6 part 3 (biochemical assay, fermentation, beta galactosidase, onpg, TSI - microbiology lab days 5 and 6 part 4 (biochemical assay, fermentation, beta galactosidase, onpg, TSI
α-Galactosidases are enzymes that act on galactosides present in many vegetables, mainly legumes and cereals, have growing importance with respect to our diet. For this reason, the use of their catalytic activity is of great interest in numerous biotechnological applications, especially those in the food industry directed to the degradation of oligosaccharides derived from raffinose. The aim of this work has been to optimize the recombinant production and further characterization of α-galactosidase of Saccharomyces cerevisiae. The MEL1 gene coding for the α-galactosidase of S. cerevisiae (ScAGal) was cloned and expressed in the S. cerevisiae strain BJ3505. Different constructions were designed to obtain the degree of purification necessary for enzymatic characterization and to improve the productive process of the enzyme. ScAGal has greater specificity for the synthetic substrate p-nitrophenyl-α-d-galactopyranoside than for natural substrates, followed by the natural glycosides, melibiose, raffinose
Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. Here we report the crystallographic structures of Penicillium sp. beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. The amino acid sequence of this 120 kDa protein was first assigned putatively on the basis of inspection of the experimental electron density maps and then determined by nucleotide sequence analysis. Primary structure alignments reveal that Penicillium sp. beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). This model is the first 3D structure for a member of GHF-35. Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. Superposition of this complex with other beta-galactosidase complexes from several hydrolase ...
β-galactosidase and β-glucuronidase enzymes are commonly used as reporters for gene expression from gene promoter-lacZ or uidA fusions (respectively). The protocol described here is a high-throughput alternative to the commonly used Miller assay (Miller, 1972) that utilises a fluorogenic substrate (Fiksdal et al., 1994) and 96-well plate format. The fluorogenic substrates 4-Methylumbelliferyl β-D-galactoside (for β-galactosidase assays) (Ramsay et al., 2013) or 4-Methylumbelliferyl β-D-glucuronide (for β-glucuronidase assays) (Ramsay et al., 2011) are cleaved to produce the fluorescent product 4-methylumbelliferone. Cells are permeabilized by freeze-thawing and lysozyme, and the production of 4-methylumbelliferone is monitored continuously by a fluorescence microplate reader as a kinetic assay. The rate of increase in fluorescence is then calculated, from which relative gene-expression levels are extrapolated. Due to the high sensitivity fluorescence-based detection of ...
BACKGROUND: The enzyme beta-galactosidase present in the Kupffer cells of the liver has potential as a marker of liver dysfunction prior to transplantation. Spectrophotometric methods have insufficient sensitivity. METHODS: Fluorimetric methods have the required sensitivity and we have optimised such a method in a microtitre plate format to improve its utility. beta-galactosidase acts on the substrate 4-methylumbelliferyl-galactoside (MUG) to produce 4-methylumbelliferone (4-MU), detected fluorimetrically with excitation wavelength 355 nm and emission wavelength 460 nm. RESULTS: Reaction conditions in a citrate-phosphate buffer were optimised to give maximal enzyme activity: pH was optimal at 4.4 (range investigated 3.6-5.0) and substrate concentration at 3.33 mmol/l. A small specimen volume (10 microl) in 80 microl of substrate solution produced adequate fluorescent yield after an incubation period of 30 to 60 min at 37 degrees C. Reaction was terminated by addition of 200 microl of glycine-NaOH, pH 12
Alpha-Lactose is the alpha anomoer of lactose. It is a disaccharide that consists of galactose and glucose joined by an acetal oxygen bridge in the beta orientation Lactose is a sugar substrate that can be readily used by E. coli. The consumption of lactose by E. coli is controlled by the lac operon. The operon includes beta-galactosidase, lactose permease, and thiogalactoside transacetylase. The lactose permease, which sits in the cytoplasmic membrane, transports lactose into the cell. Beta-galactosidase, a cytoplasmic enzyme, subsequently cleaves lactose into glucose and galactose ...
Alpha galactosidase A Antibody 66121-1-Ig has been identified with IF, IHC, WB, ELISA. 66121-1-Ig detected 49 kDa band in HeLa cells with 1:500-1:2000 dilution...
Alpha galactosidase A Antibody 19877-1-AP has been identified with IF, WB, ELISA. 19877-1-AP detected 49 kDa band in HEK-293 cells with 1:500-1:3000 dilution...
a The β-galactosidase activity assay was performed as described in the MATCHMAKER two-hybrid system (20). Briefly, cells from 1.5-ml samples of exponential culture were collected by centrifugation and resuspended in 300 μl of Z-buffer (20). A 100-μl aliquot of the resuspended cells was lysed by quick freeze-thaw (treatment with liquid nitrogen followed by thawing at 37°C). To measure the β-galactosidase activity in the cell lysate, a 0.7-ml sample of the Z-buffer-β-mercaptoethanol solution was added to each tube followed by 0.16 ml of Z-buffer-ONPG (4 mg of ONPG per 1 ml of Z-buffer). The time of ONPG addition was recorded, and the tubes were incubated at 30°C with shaking. When yellow color was visible, 400 μl of 1 M NaCO3 was added to each tube to terminate the reaction, and the time was recorded. The tubes were then centrifuged for 10 min at 10,000 × g to remove cellular debris, and the optical density at 420 nm was recorded. β-Galactosidase units were defined as the amount of ...
Effect of pH on Lactase Science Experiments Elementary, 2013 Science Experiments, Science Fair Projects,Science Fair Ideas Experiments, Kids Project Experiment Ideas, science experiment projects, kids experiments science, children simple,cool,fun and easy science fair experiments,topics for science experiments, and also for Middle school, Elementary School for class 5th Grade,6th,7th,8th,9th 10th,11th, 12th Grade and High School , MSC and College Students.
Neste trabalho foi desenvolvido um novo bioprocesso para a síntese de lactosacarose, um candidato a prebiótico. A lactosacarose foi produzida por transgalactosilação, catalisada pela β-galactosidase de Bacillus circulans imobilizada em macroesfera de quitosana, utilizando a lactose e a sacarose como substratos. No processo de imobilização, os resultados indicam que a melhor razão entre a concentração de enzima e de suporte foi de 200 mg.g-1. A estabilidade térmica da enzima imobilizada foi determinada e comparada com a estabilidade térmica da enzima livre em temperaturas de 50, 60 e 70 °C e para esta última foi verificada a estabilidade na presença e ausência de substrato. A imobilização aumentou de 10 a 260 vezes a estabilidade térmica da enzima, sendo este efeito inversamente relacionado com a temperatura. A otimização das condições de produção indica, para a β-galactosidase imobilizada e livre, que a melhor condição de produção de lactosacarose e de ...
Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. Original antibody is raised against beta-galactosidase. (RAB00031) - Products - Abnova
After a short introduction to the who/what/why for this class, well start handling the programming material thats at our fingertips...in our fingertips, even! As you work through this DNA isolation protocol below, be mindful of your questions and your own reactions to this activity. Is this the first time youve ever handled a lump of DNA? How hard is it to isolate DNA, both technically speaking and in terms of the needed equipment? What surprised you? What did you learn and what questions do you still have? What could you do next with this material youve isolated? ...
Otsuka Novel Products GmbH (ONPG) is dedicated to finding innovative solutions to fight the global pandemic of tuberculosis (TB). ONPG focuses on providing access to innovative products for the treatment of TB and working to stop the spread of antimicrobial resistance.
Otsuka Novel Products GmbH (ONPG) is dedicated to finding innovative solutions to fight the global pandemic of tuberculosis (TB). ONPG focuses on providing access to innovative products for the treatment of TB and working to stop the spread of antimicrobial resistance.
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Beta-galactosidase, 0.1 ml. This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates.
Save today on Lactase Enzyme 180 tab from Best Naturals, VitaNet carries a large selection of Best Naturals products and other brands at discount prices call today 1-800-877-8702.
Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C Beta-Galactosidase (E. Coli) Protein for Western Blot Western control BGAL11-C
Alpha-galactosidase (EC 3.2.1.22) (melibiase) catalyzes the hydrolysis of melibiose into galactose and glucose. In man, the ... Escherichia coli alpha-galactosidase (gene melA), which requires NAD and magnesium as cofactors, is not structurally related to ... The sequence of this enzyme is highly related to that of the eukaryotic alpha-galactosidases. This family also includes ... Glycoside hydrolase family 36 together with family 31 and family 27 alpha-galactosidases form the glycosyl hydrolase clan GH-D ...
Dey PM, Pridham JB (1972). "Biochemistry of α-Galactosidases". Biochemistry of alpha-galactosidases. Adv. Enzymol. Relat. Areas ... Alpha-galactosidase (EC 3.2.1.22) (melibiase) catalyzes the hydrolysis of melibiose into galactose and glucose. In man, the ... Escherichia coli alpha-galactosidase (gene melA), which requires NAD and magnesium as cofactors, is not structurally related to ... The sequence of this enzyme is highly related to that of the eukaryotic alpha-galactosidases. GH27 in CAZypedia Henrissat B, ...
A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. This enzyme causes ... Smale, S. T. (2010-05-01). "-Galactosidase Assay". Cold Spring Harbor Protocols. 2010 (5): pdb.prot5423. doi:10.1101/pdb. ... Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity. ... in the β-galactosidase system. As a result, the reporter gene's expression is independent of the gene of interest's expression ...
Most patients have a normal size liver and spleen.Prenatal diagnosis is possible by measurement of Acid Beta Galactosidase in ... The GM1 gangliosidoses are caused by a deficiency of beta-galactosidase, with resulting abnormal storage of acidic lipid ... Autosomal recessive disorder; beta-galactosidase deficiency; neuronal storage of GM1 ganglioside and visceral storage of ... Mutations with higher residual beta-galactosidase activity for the GM1 substrate than for keratan sulfate and other galactose- ...
Domain knowledge (P) feed(lactose) :- make(permease), make(galactosidase). make(Enzyme) :- code(Gene, Enzyme), express(Gene). ... The two enzymes of permease and galactosidase are coded by two genes, lac(y) and lac(z) respectively (stated in the fifth and ... coli can feed on the sugar lactose if it makes two enzymes permease and galactosidase. Like all enzymes, these are made if they ... galactosidase). temperature(low) :- amount(glucose, low). Integrity constraints (IC) false :- amount(S, V1), amount(S, V2), V1 ...
... and is commonly used as a substrate for assay of β-galactosidase in vitro. Colonies that produce β-galactosidase are turned ... lacZ encodes β-galactosidase (LacZ), an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. ... Phenyl-β-D-galactose (phenyl-Gal) is a substrate for β-galactosidase, but does not inactivate repressor and so is not an ... "ONPG (β-Galactosidase) test". September 2000. Archived from the original on 3 November 2007. Retrieved 25 October 2007. Joung J ...
... beta-galactosidase (EC 3.2.1.23); 6-phospho-beta-galactosidase (EC 3.2.1.85); 6-phospho-beta-glucosidase (EC 3.2.1.86); lactase ... 6-phospho-beta-galactosidase InterPro: IPR005928 GBA3; KL; KLB; LCT; LCTL; GH1 in CAZypedia Henrissat B, Callebaut I, Fabrega S ...
nov., a beta-galactosidase-producing gammaproteobacterium". The Journal of General and Applied Microbiology. 50 (3): 143-7. doi ... Rhodanobacter fulvus produce beta-galactosidase. Parte, A.C. "Rhodanobacter". LPSN. "Rhodanobacter fulvus". www.uniprot.org. ...
... alpha-galactosidase (EC 3.2.1.22). 6-phospho-alpha-glucosidase requires both NAD(H) and divalent metal (Mn2+, Fe2+, Co2+, or ...
Zhang, B.; Chen, Y.; Li, Z.; Lu, W.; Cao, Y. (2011). "Cloning and functional expression of α-galactosidase cDNA from ... Q. Ashton Acton (2013). Galactosidases-Advances in Research and Application: 2013 Edition: ScholarlyBrief. ScholarlyEditions. ...
... endo-beta-galactosidase (EC 3.2.1.103). Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). " ...
nov., a beta-galactosidase-producing gammaproteobacterium". The Journal of General and Applied Microbiology. 50 (3): 143-7. doi ...
β-galactosidase activity of a PsuhB::lacZ fusion confirmed the lower expression of suhB in TFA cells growing at low rates. A ...
The side chain can be cleaved by galactosidases and glucosidases. The deficiency of α-galactosidase A causes Fabry's disease, ... Germain, D. P. (2002). "Fabry's disease (alpha-galactosidase-A deficiency): Physiopathology, clinical signs, and genetic ...
"Entrez Gene: Galactosidase, beta 1-like 3". Retrieved 2012-07-24. Taylor TD, Noguchi H, Totoki Y, Toyoda A, Kuroki Y, Dewar K, ... Galactosidase, beta 1-like 3 is a protein in humans that is encoded by the GLB1L3 gene. GRCh38: Ensembl release 89: ...
β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ... The presence of an active β-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by β- ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ... The method is based on the principle of α-complementation of the β-galactosidase gene. This phenomenon of α-complementation was ...
List of ineffective cancer treatments Galactosidases - Advances in Research and Application. Scholarly Editions. 21 June 2013. ...
... galactosidases, glucosidases and mannosidases". J. Am. Chem. Soc. 65: 1369-1380. doi:10.1021/ja01247a029. Inulinase at the US ...
... galactosidases, glucosidases and mannosidases". J. Am. Chem. Soc. 65 (7): 1369-1380. doi:10.1021/ja01247a029. Bartholomew, B.A ...
"Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences". PLOS ONE. 12 (3): e0172545. ...
The thermostable enzyme β-galactosidase isolated from the extreme thermophile archaebacterial Sulfolobus solfataricus, strain ... M. PISANI, Francesca (1990). "Thermostable P-galactosidase from the archaebacterium Sulfolobus solfataricus". European Journal ...
It contains the enzyme alpha-galactosidase (α-GAL). It was introduced as a liquid, but that has been discontinued and it is now ... "The effect of oral alpha-galactosidase on intestinal gas production and gas-related symptoms". Dig. Dis. Sci. 52 (1): 78-83. ... A double-blind crossover study of oral alpha-galactosidase to treat dietary oligosaccharide intolerance". J Fam Pract. 39 (5): ...
1991). "Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases". ... Chakraborty S, Rafi MA, Wenger DA (1994). "Mutations in the lysosomal beta-galactosidase gene that cause the adult form of GM1 ... 1991). "Combined deficiency of beta-galactosidase and neuraminidase: natural history of the disease in the first 18 years of an ... 1984). "Morquio B syndrome: a primary defect in beta-galactosidase". Am. J. Med. Genet. 16 (2): 261-75. doi:10.1002/ajmg. ...
weissbach, H (1977). "DNA-directed in vitro synthesis of beta-galactosidase. Studies with purified factors". The Journal of ...
Niacin negative, catalase positive, β-galactosidase usually positive. Tween 80 not hydrolysed after 14 days. Resistant to 5% ...
Enzymes that break the β-galactoside glycosidic bond are called β-galactosidases. [1] v t e. ...
Two distinct β-galactosidase-activated contrast agents have been reported. Both consist of a Gd(III) ion complexed with a ... To date, β-galactosidase-activated contrast agents have attracted the most attention in the literature, although there no ... In a tissue where active β-galactosidase is present, the sugar will be cleaved from the rest of the compound. This permits ... In a research context, the α-series of β-galactosidase-activated MR contrast agents has been used to visualize the development ...
The bacterial lacZ gene encodes a beta-galactosidase enzyme. When media containing certain galactosides (e.g. X-gal), cells ...
The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. The sugar ... Jacobson RH, Zhang XJ, DuBose RF, Matthews BW (June 1994). "Three-dimensional structure of beta-galactosidase from E. coli". ... Glycoside hydrolase family 2 comprises enzymes with several known activities: beta-galactosidase (EC 3.2.1.23); beta- ... beta-galactosidase from Escherichia coli". The Journal of Biological Chemistry. 267 (16): 11126-30. PMID 1350782. ...
B-galactosidase forms the basis of lac z operon in bacteria which can be used to control gene expression. B-galactosidase can ... B-galactosidase is made up of 4 identical polypeptide chains, i.e. it has 4 identical subunits. When B-galactosidase is ... Lack of alpha-galactosidase activity in leukocytes has been linked to Fabry Disease. Galactosidases have a variety of uses, ... producing the alpha fragment and allowing for B-galactosidase to gain its activity. To trace the activity of B-galactosidase a ...
Beta-galactosidase has many homologues based on similar sequences. A few are evolved beta-galactosidase (EBG), beta-glucosidase ... The EC (Enzyme Commission) number of β-galactosidase is 3.2.1.23 β-galactosidase belongs to class 3, which refers to the ... April 2006). "Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111 ... the cells therefore show no β-galactosidase activity. The presence or absence of an active β-galactosidase may be detected by X ...
Galactosidase definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and translation. Look it up ... galactosidase in Medicine Expand. galactosidase ga·lac·to·si·dase (-gə-lāktō-sĭ-dās, -dāz, -lāk-tō-). n. Any of a group of ...
A list of US medications equivalent to Beta-Galactosidase (Penicillium) is available on the Drugs.com website. ... Beta-Galactosidase (Penicillium) is a medicine available in a number of countries worldwide. ... Beta-Galactosidase (Penicillium) may be available in the countries listed below.. Ingredient matches for Beta-Galactosidase ( ...
immunogen = β-Galactosidase purified from E. coli. Specificity. reacts only with β-galactosidase from E. coli, no reactivity is ...
immunogen = β-Galactosidase from E. coli. Specificity. reacts with β- Galactosidase; specially prepared for the detection and ...
β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 ... β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 ... β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 ... Unit Definition Assay: Two fold dilutions of β1-3 Galactosidase are incubated with 1 nmol AMC-labeled substrate in 1X ...
This domain is found in beta-galactosidase enzymes, mainly from the glycosyl hydrolase 35 family. It has a jelly roll fold [ ... Beta-galactosidase jelly roll domain (IPR025300). Short name: BetaGal_jelly_roll_dom ... Crystal structures of beta-galactosidase from Penicillium sp. and its complex with galactose.. J. Mol. Biol. 343 1281-92 2004 ...
Alpha-Galactosidase - Last updated on May 5, 2021. All rights owned and reserved by Memorial Sloan Kettering Cancer Center. ...
... Martin Kennedy mkennedy at chmeds.ac.nz Tue Oct 5 21:23:18 EST 1993 *Previous message: ...
The SV40 early promoter and enhancer drive transcription of the lacZ gene, which encodes the β-galactosidase enzyme. ... The pSV-β-Galactosidase Vector will express β-galactosidase in E. coli due to the presence of the E. coli gpt promoter located ... β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer. Absorbance-based detection of β-galactosidase activity in ... which encodes the β-galactosidase enzyme. β-galactosidase is an excellent reporter enzyme that can be assayed quickly and ...
... Justin Kemp justin.news.invalid at web2news.net Wed Feb 26 23:31:21 EST 2003 *Previous ... I have been told that beta-galactosidase may be suitable. Can anyone tell me if it is indeed devoid of carbohydrate? Any help ...
The basic idea here is to create an innocuous virus that carries the lac gene, which encodes the enzyme β-galactosidase, or ... Posted in Medical HacksTagged adenovirus, beta-galactosidase, biohacking, enzyme, genetic engineering, lactase, lactose ...
β-Galactosidase Assay. The β-galactosidase activities of the commercial enzymes were assayed using o-nitrophenyl-β-D- ... β-galactosidase than for K. lactis β-galactosidase. Figures 1, 2, and 3 show that the lactose hydrolysis of milk, whey, and ... β-galactosidase. These enzymatic concentrations corresponded to 0.1, 0.2, and 0.3 g/L of A. oryzae β-galactosidase and to 0.37 ... β-galactosidases, both in 3, 6, and 9 U/mL concentrations. In the temperature of 10°C, the K. lactis β-galactosidase enzyme is ...
Suicide gene therapy using E. coli beta-galactosidase.. Farquhar D1, Pan BF, Sakurai M, Ghosh A, Mullen CA, Nelson JA. ... The purpose of this study was to investigate a novel suicide gene therapy using E. coli beta-galactosidase (beta-gal) as the ...
Alpha-galactosidase A. A, B. 398. Homo sapiens. Mutation(s): 0 Gene Names: GLA. EC: 3.2.1.22 (PDB Primary Data), 3.2.1.47 ( ... The molecular defect leading to Fabry disease: structure of human alpha-galactosidase. Garman, S.C., Garboczi, D.N.. (2004) J ... Deficiency in the lysosomal enzyme alpha-galactosidase (alpha-GAL) causes an accumulation of its substrate, which ultimately ... Deficiency in the lysosomal enzyme alpha-galactosidase (alpha-GAL) causes an accumulation of its substrate, which ultimately ...
... appears to be the deficiency of galactocerebroside β-galactosidase (galactosylceramide galactosyl hydrolase). Among... ... Bowen, D. M. and Radin, N. S.: Cerebroside Galactosidase: A Method for Determination and a Comparison with Other Lysosomal ... Suzuki K., Suzuki Y., Fletcher T.F. (1972) Further Studies on Galactocerebroside β-Galactosidase in Globoid Cell Leukodystrophy ... Radin, N. S., Hof, L., Bradley, R. M. and Brady, R. O.: Lactosylceramide Galactosidase: Comparison with Other Sphingolipid ...
GanA; Beta-galactosidase GanA [Carbohydrate transport and metabolism]. * XM_011542572.1 → XP_011540874.1 beta-galactosidase-1- ... BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542566.2 → XP_011540868.1 beta-galactosidase-1-like protein 3 ... BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542569.2 → XP_011540871.1 beta-galactosidase-1-like protein 3 ... BetaGal_dom4_5; Beta-galactosidase jelly roll domain. * XM_011542570.2 → XP_011540872.1 beta-galactosidase-1-like protein 3 ...
Detailed Alpha-D-Galactosidase dosage information for adults. Includes dosages for Flatulence; plus renal, liver and dialysis ... Alpha-D-galactosidase oral drops:. Take 5 drops/problem food right before your first bite. A typical meal has 3 servings of ... Alpha-D-galactosidase oral tablet/capsule, chewable:. Chew or swallow whole 1 tablet/capsule per problem food right before your ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
"Galactosidases" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Galactosidases" by people in Harvard Catalyst Profiles by year ... Below are the most recent publications written about "Galactosidases" by people in Profiles. ... and whether "Galactosidases" was a major or minor topic of these publication. ...
Beta-galactosidaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... tr,O31341,O31341_BACCI Beta-galactosidase OS=Bacillus circulans GN=bgaC PE=1 SV=1 ...
Chicken polyclonal beta Galactosidase antibody. Validated in WB and tested in Escherichia coli. Immunogen corresponding to ... All lanes : Anti-beta Galactosidase antibody (ab106567) at 1 µg/ml. Lane 1 : beta Galactosidase at 0.005 µg. Lane 2 : beta ... Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose ... 17 amino acid peptide from near the N terminus of beta Galactosidase from E. coli (NP_752394). ...
Fabry disease: isolation of a cDNA clone encoding human alpha-galactosidase A. D H Calhoun, D F Bishop, H S Bernstein, M Quinn ... Fabry disease: isolation of a cDNA clone encoding human alpha-galactosidase A ... Fabry disease: isolation of a cDNA clone encoding human alpha-galactosidase A ... Fabry disease: isolation of a cDNA clone encoding human alpha-galactosidase A ...
Drops; Oral; Alpha-Galactosidase 150 galu / 5 drop*Tablets; Oral; Alpha-Galactosidase 13.8 mg. ...
... can produce the enzyme β-galactosidase which breaks lactose into galactose and glucose. However, the gene for β-galactosidase ... Class practical Escherichia coli (E. coli) can produce the enzyme β-galactosidase which breaks lactose into galactose and ... Gene induction: ß-galactosidase in E. coli Class practical. Escherichia coli (E. coli) can produce the enzyme β-galactosidase ... 3 E. coli: use a strain with the lacZ (β-galactosidase) gene, for example, the K12 strain. Make up the culture 24-48 hours ...
β-galactosidase is found in plants, animals and microorganisms. In Beta-Galactosidase: Properties, Structure and Functions, the ... Beta-Galactosidase: Properties, Structure and Functions. Eloy Kras (Editor). Series: Cell Biology Research Progress. BISAC: ... β-galactosidase is an enzyme responsible for catalyzing the hydrolysis of the lactose β-1,4 linkage into α-D-glucose and β-D- ... An efficient β-galactosidase producing strain SYA2 screened out from 21 strains was identified as Enterobacter sp. by means of ...
Rabbit polyclonal GLB1/Beta-galactosidase antibody. Validated in IHC and tested in Mouse, Rat, Human. Cited in 5 publication(s ... Primary - Rabbit Anti-GLB1/Beta-galactosidase antibody (ab203749) IHC-P Protein - Recombinant Human GLB1/Beta-galactosidase ... Anti-GLB1/Beta-galactosidase antibody. See all GLB1/Beta-galactosidase primary antibodies. ... Hall BM et al. Aging of mice is associated with p16(Ink4a)- and ß-galactosidase-positive macrophage accumulation that can be ...
Bluo-gal and X-gal are commonly used substrates for measuring enzymatic activity of beta-galactosidase in bacteria. Life ... Bluo-gal and X-gal are commonly used substrates for measuring enzymatic activity of beta-galactosidase in bacteria. Life ...
α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids[1]. β-Galactosidase (Bgal ... For details on β-galactosidase see Molecular Playground/Beta-galactosidase. Relevance Bgal assay is used in molecular biology ... The molecular defect leading to Fabry disease: structure of human alpha-galactosidase. J Mol Biol. 2004 Mar 19;337(2):319-35. ... Fabrys disease: alpha-galactosidase deficiency. Science. 1970 Feb 27;167(3922):1268-9. PMID:5411915 ...
  • Geller AI, Freese A. Infection of cultured central nervous system neurons with a defective herpes simplex virus 1 vector results in stable expression of Escherichia coli beta-galactosidase. (harvard.edu)
  • Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. (abcam.com)
  • Escherichia coli ( E. coli ) can produce the enzyme β-galactosidase which breaks lactose into galactose and glucose. (nuffieldfoundation.org)
  • Structure of Escherichia coli β-galactosidase (∼465 kDa) at 3.2 Å resolution solved using the GIF Quantum LS energy filter that includes a K2 Summit camera. (gatan.com)
  • Activation of a defective Escherichia coli β-D-galactosidase by specific activating antibody is inhibited competitively by a molecule with immunoglobulin properties but devoid of activating capacity. (jimmunol.org)
  • Beta-Galactosidase from Escherichia coli . (abnova.com)
  • Recombinant Escherichia coli β-galactosidase (β- d -galactoside galactohydrolase, EC 3.2.1.23) with a specific activity of 700 to 1200 units/mg protein was purchased from Roche Diagnostics (Indianapolis, IN). (aspetjournals.org)
  • 1. Michaelis-Menten parameters for the hydrolysis of 4-nitrophenyl β-D-galactopyranoside and 3,4-dinitrophenyl β-D-galactopyranoside Escherichia coli (lacZ) β-galactosidase were measured as a function of pH or pD (pL) in both 1H2O and 2H2O. (biochemj.org)
  • Here we report the structure of a complex between Escherichia coli β-galactosidase and the cell-permeant inhibitor phenylethyl β-D-thiogalactopyranoside (PETG), determined by cryo-EM at an average resolution of ~2.2 angstroms (Å). (pdbj.org)
  • Anti Beta-galactosidase antibody recognizes the enzyme beta-galactosidase, or ß-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. (acris-antibodies.com)
  • Anti Beta-galactosidase antibody is ideal for investigators involved in enzyme research. (acris-antibodies.com)
  • Western blotting using Fluorescein conjugated anti-Beta-galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of b-Gal present in partially purified preparations (arrowhead). (acris-antibodies.com)
  • WB analysis of beta Galactosidase protein (lanes1-3 for 60 ng, 30 ng and 15 ng, respectively) and non-specific control E.coli lysate (lane 4) using beta Galactosidase antibody. (acris-antibodies.com)
  • WB analysis of beta Galactosidase protein (1 μg) using beta Galactosidase antibody at a dilution of 1:1,000. (acris-antibodies.com)
  • WB analysis of non-reduced and reduced beta Galactosidase protein (lane1 for 80 ng and lane 2 for 20 ng) using beta Galactosidase antibody at a dilution of 1:10,000. (acris-antibodies.com)
  • Western Blot: beta Galactosidase Antibody [FITC] [NB120-6641] - Gal present in a partially purified preparation (arrowhead). (acris-antibodies.com)
  • Original antibody is raised against beta-galactosidase. (abnova.com)
  • Mouse monoclonal antibody raised against Beta-Galactosidase. (abnova.com)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta galactosidase antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta galactosidase antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9). (ptglab.com)
  • B-galactosidase forms the basis of lac z operon in bacteria which can be used to control gene expression. (wikipedia.org)
  • When the target gene is not found in the vector, the alpha fragment gene would be active, producing the alpha fragment and allowing for B-galactosidase to gain its activity. (wikipedia.org)
  • Therefore, when the bacteria is transformed with the recombinant plasmid B-galactosidase is inactive and the colonies appear white, but when bacteria are transformed with the original plasmid, lacking the target gene, B-galactosidase is active and the colonies appear blue. (wikipedia.org)
  • In recent years, beta-galactosidase has been researched as a potential treatment for lactose intolerance through gene replacement therapy where it could be placed into the human DNA so individuals can break down lactose on their own. (wikipedia.org)
  • The basic idea here is to create an innocuous virus that carries the lac gene, which encodes the enzyme β-galactosidase, or lactase, and use it to infect the cells of his small intestine. (hackaday.com)
  • Suicide gene therapy using E. coli beta-galactosidase. (nih.gov)
  • The purpose of this study was to investigate a novel suicide gene therapy using E. coli beta-galactosidase (beta-gal) as the prodrug-activating enzyme. (nih.gov)
  • However, the gene for β-galactosidase is normally switched off, except in the presence of lactose. (nuffieldfoundation.org)
  • The procedure indicates that the gene which produces β-galactosidase in E. coli is induced or 'switched on' by the presence of lactose. (nuffieldfoundation.org)
  • use a strain with the lacZ (β-galactosidase) gene, for example, the K12 strain. (nuffieldfoundation.org)
  • We offer two ways to detect LacZ gene activity: a colorimetric beta-galactosidase staining kit and a chemiluminescent beta-galactosidase assay. (clontech.com)
  • In this kit, beta-galactosidase (the product of the LacZ gene) catalyzes the hydrolysis of X-gal to produce a blue color that is easily visualized. (clontech.com)
  • Beta-galactosidase is the product of the LacZ gene. (clontech.com)
  • Alpha-galactosidase deficiency (Fabry disease, Fabry syndrome, Anderson-Fabry syndrome) is caused by gene variations that result in deficiency in lysosomal alpha-galactosidase . (ganfyd.org)
  • The gene product of lac Z, β-galactosidase, catalyzes the hydrolysis of X-gal, which produces a blue color that is easily visualized. (activemotif.com)
  • A mutant with an internal deletion of codons 21-41 of the lacZ gene does not produce any active β-galactosidase. (openwetware.org)
  • A gene on chromosome 3p21.33 that encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyses the terminal beta-galactose from gangliosides and other glycoconjugates. (thefreedictionary.com)
  • The disorder is "caused by mutations in the GLB1 gene, which encodes an enzyme called beta-galactosidase necessary for recycling of a molecule (GM1-ganglioside) in neurons. (thefreedictionary.com)
  • The objects of this invention are new Saccharomyces cerevisiae yeast strains into which α-galactosidase gene (MEL + ) has been transferred by using recombinant DNA methods. (freepatentsonline.com)
  • The trap vectors contain a reporter gene, typically β-galactosidase (β-gal), that is not expressed unless it integrates into an intron or exon of a transcription unit. (pnas.org)
  • b-galactosidase, a widely used reporter enzyme, is used in the study of gene expression, protein-protein interactions,1 and normalization of transfection efficiency.2 -galactosidase, enzyme encoded by the lacZ gene of E. coli, catalyzes the hydrolysis of -galactosides into monosaccharides. (hum-molgen.org)
  • This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the termil beta-galactose from ganglioside substrates and other glycoconjugates. (acris-antibodies.com)
  • Here, we identified a gene agabb from B. bifidum JCM 1254, which encodes glycoside hydrolase (GH) family 110 α-galactosidase. (sigmaaldrich.com)
  • New heat shock puffs and beta‐galactosidase activity resulting from transformation of Drosophila with an hsp70‐lacZ hybrid gene. (currentprotocols.com)
  • Histochemical staining of clonal mammalian cell lines expressing E. coli beta galactosidase indicates heterogeneous expression of the bacterial gene. (currentprotocols.com)
  • A novel β-galactosidase (β-gal) gene was cloned from Bacillus circulans ATCC 31382. (go.jp)
  • Our aim was to elucidate the possible clinical diagnosis and mechanisms leading to WML in patients carrying the D313Y mutation in the α-galactosidase A (GLA) gene, a mutation that was formerly described as nonpathogenic. (ebscohost.com)
  • The Galacto-Light™ beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of b-galactosidase in reporter gene assays. (selectscience.net)
  • In this study, a deoxyribozyme against α-peptide beta-galactosidase gene in the lactose operon was designed. (magiran.com)
  • Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene. (asm.org)
  • The P10 promoter directed the synthesis of beta-galactosidase, whereas the polyhedrin promoter controlled the synthesis of foreign gene products. (asm.org)
  • These two genes recombined with wild-type virus genome to yield recombinants which were polyhedrin negative, produced the foreign gene product, and formed blue plaques when beta-galactosidase indicator was present in the agarose overlay. (asm.org)
  • On the other hand, if it is a beta-galactoside, it is called beta-galactosidase, and is responsible for breaking down the disaccharide lactose into its monosaccharide components, glucose and galactose Both varieties of galactosidase are categorized under the EC number 3.2.1. (wikipedia.org)
  • Galactosidases have a variety of uses, including the production of prebiotics, the biosynthesis of transgalactosylated products, and the removal of lactose. (wikipedia.org)
  • To trace the activity of B-galactosidase a colorless analog of lactose is used, X-gal. (wikipedia.org)
  • β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose. (wikipedia.org)
  • This work aimed at evaluating the influence of enzyme concentration, temperature, and reaction time in the lactose hydrolysis process in milk, cheese whey, and whey permeate, using two commercial β -galactosidases of microbial origins. (hindawi.com)
  • In the temperature of 10°C, the K. lactis β -galactosidase enzyme is more efficient in the milk, cheese whey, and whey permeate lactose hydrolysis when compared to A. oryzae . (hindawi.com)
  • However, in the enzyme reaction time and concentration conditions evaluated, 100% lactose hydrolysis was not reached using the K. lactis β -galactosidase. (hindawi.com)
  • The studied parameters in the lactose enzymatic hydrolysis are critical for enabling the application of β -galactosidases in the food industry. (hindawi.com)
  • however, for most of them, the β -galactosidase activity decreases after the said period, which characterizes primary hypolactasia and brings about symptoms of lactose intolerance. (hindawi.com)
  • In this procedure, a sample of E. coli is treated with lactose, and then the β-galactosidase activity of this sample and an untreated sample are compared. (nuffieldfoundation.org)
  • A sensitive, selectable marker system has been developed for fluorescent pseudomonads based on expression of the E. coli lac operon genes encoding β-galactosidase and lactose permease. (nature.com)
  • The beta-galactosidase forms were heat-stable up to about 60 degrees C. The Km values for lactose ranged from 85 mM to 125 mM, whereas those for the synthetic substrate o-nitrophenyl-beta-D-galactopyranoside were equal to about 2.4 mM. (nih.gov)
  • β-Galactosidase enzymes are used in the dairy industry to convert lactose into galactooligosaccharides (GOS) that are added to infant formula to mimic the molecular sizes and prebiotic functions of human milk oligosaccharides. (nih.gov)
  • This ultrasonification technique, in which the yogurt bacteria are ruptured to release more galactosidase and improve the viability of probiotics, should encourage more consumers to buy yogurt, which exerts health benefits beyond inherent basic nutrition as it improves lactose absorption in the human gut. (thefreelibrary.com)
  • The enzyme β-galactosidase (EC 3.2.1.23) can synthesize GOS from lactose (transgalactosylation reaction), but also hydrolyze GOS and lactose (hydrolysis reaction). (omicsonline.org)
  • Le β galactoside plus commun et connu en biochimie est le lactose. (academic.ru)
  • Enzymologists have recently attributed great importance to the galactosyltransferase activity of b -galactosidases to obtain the synthesis of oligosaccharides with 2 or more galactose units starting from lactose. (scielo.br)
  • Solutions with high lactose concentrations, such as evaporated milk whey, submitted to the action of b -galactosidase from some microorganisms suffer a transgalactosylation reaction, producing a galactooligosaccharide mixture or transgalactosylated oligosaccharides (TOS) which act as functional foods, with several beneficial effects for their consumers. (scielo.br)
  • This book describes the reaction rate profiles of the beta-galactosidase-catalyzed conversion of lactose on the inner surface of a hollow fiber membrane, which is employed as an enzymatic reactor system. (weltbild.ch)
  • Previously, an increase in performance of 'β-galactosidase-catalyzed conversion of lactose assisted by ultrafiltration was suggested due to inhibitor removal.However, as the analysis presented here shows, the concentration profile of the substrate on the membrane surface also affects the reaction performance. (springer.com)
  • METHODS: Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was applied to evaluate the influence of five factors (peanut pod concentration, C/N ratio, incubation time, type of solid substrate and lactose concentration) on the β-galactosidase production by Bacillus licheniformis under solid-state fermentation. (minervamedica.it)
  • The hydrolysis of X-gal by B-galactosidase produces galactose, a blue colored compound. (wikipedia.org)
  • β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. (wikipedia.org)
  • β1-3 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of β1-3 and, at a much lower rate, β1-6 linked galactose residues from oligosaccharides. (neb.com)
  • Thus, sugar enzymatic hydrolysis from the β -galactosidase enzyme, generating glucose and galactose monosaccharides, emerges as a key biotechnological process with application in the dairy industry. (hindawi.com)
  • Hidaka M, Fushinobu S, Ohtsu N, Motoshima H, Matsuzawa H, Shoun H, Wakagi T. Trimeric crystal structure of the glycoside hydrolase family 42 beta-galactosidase from Thermus thermophilus A4 and the structure of its complex with galactose. (proteopedia.org)
  • It is an autosomal recessive sphingolipidosis caused by deficient activity of the lysosomal enzyme galactosylceramide beta-galactosidase (GALC) which degrades galactosylceramide, a main component of myelin, and other terminal beta-galactose-containing sphingolipids, including psychosine (galactosylsphingosine). (thefreedictionary.com)
  • β1-4 Galactosidase S will only cleave galactose residues with a β1-4 linked glycosidic bond, while β1-3 Galactosidase will cleave galactose residues with β1-3 and, at a lower efficiency, β1-6 linked glycosidic bonds. (neb.com)
  • beta-galactosidase and its complex with galactose solved by the SIRAS quick cryo-soaking technique at 1.90 A and 2.10 A resolution, respectively. (uzh.ch)
  • The membrane was probed with Fluorescein conjugated anti-Beta-galactosidase (Cat. (acris-antibodies.com)
  • A sensitive micro-immunoassay using beta-galactosidase/anti-beta-galactosidase complexes. (abnova.com)
  • The molecular defect leading to Fabry disease: structure of human alpha-galactosidase. (proteopedia.org)
  • The functional role of glutamine-280 and threonine-282 in human alpha-galactosidase. (springer.com)
  • β-galactosidase, also called lactase, beta-gal or β-gal, is a family of glycoside hydrolase enzymes that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. (wikipedia.org)
  • A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase-phlorizin hydrolase. (wikipedia.org)
  • The pH-dependence of the reaction kinetics of lactase (b-galactosidase) [9031-11-2] from A. oryzae in different reaction media is presented in terms of a 2-parameter model. (epfl.ch)
  • Three-dimensional structure of beta-galactosidase from E. coli. (proteopedia.org)
  • EMDB-2984: 2.2 A resolution cryo-EM structure of beta-galactosidase in compl. (pdbj.org)
  • EMDB-8908: Cryo-EM structure of beta-galactosidase using RELION on Amazon We. (pdbj.org)
  • Altered expression of β-galactosidase-1-like protein 3 (Glb1l3) in the retinal pigment epithelium (RPE)-specific 65-kDa protein knock-out mouse model of Leber's congenital amaurosis. (nih.gov)
  • The remaining lysates prepared with M-PER Reagent may be used for applications such as reporter assays (luciferase, β-galactosidase and chloramphenical acetyltransferase), protein kinase assays (PKA, PKC, and Tyrosine Kinase), immunoassays (Western blot, ELISA, RIA) and protein purification. (thermofisher.com)
  • The kit can also be used to determine the efficiency of direct delivery of β-galactosidase by the Chariot™ Protein Delivery Reagent (Figure 1). (activemotif.com)
  • One µg of a 119 kDa subunit of β-galactosidase was complexed with Chariot™ Protein Delivery Reagent for 30 minutes and delivered into HeLa cells. (activemotif.com)
  • A β‐galactosidase hybrid protein targeted to nuclei as a marker for developmental studies. (currentprotocols.com)
  • Beta-galactosidase - protein Name = caption = β galactosidase from Penicillum sp. (academic.ru)
  • A chimaeric protein confaining the first 178 amino acids from the N-terminal half of S. cerevisiae FruP2ase fused to E. coliβ-galactosidase was susceptible to catabolite inactivation. (csic.es)
  • The EC (Enzyme Commission) number of β-galactosidase is 3.2.1.23 β-galactosidase belongs to class 3, which refers to the hydrolases. (wikipedia.org)
  • The enzyme beta-galactosidase (EC 3.2.1.23) from Aspergillus niger was purified and resolved into three multiple forms, using molecular sieving, ion-exchange, an hydrophobic chromatography. (nih.gov)
  • An enzyme marker was similarly prepared by coupling 7AMD with β- d -galactosidase (EC 3.2.1.23) via N -maleoylaminobutyric acid. (aacrjournals.org)
  • This domain is found in beta-galactosidase enzymes, mainly from the glycosyl hydrolase 35 family. (ebi.ac.uk)
  • The genetic cause underlying globoid cell leukodystrophy (Krabbe's disease) appears to be the deficiency of galactocerebroside β -galactosidase (galactosylceramide galactosyl hydrolase). (springer.com)
  • Alpha-galactosidase - is a glycoside hydrolase enzyme that hydrolyses the terminal alpha galactosyl moieties from glycolipids and glycoproteins. (academic.ru)
  • Previously, we identified an α-galactosidase (Aga1) belonging to glycoside hydrolase (GH) family 36 from R. gnavus E1 (M. Aguilera, H. Rakotoarivonina, A. Brutus, T. Giardina, G. Simon, and M. Fons, Res. (asm.org)
  • β-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. (creative-enzymes.com)
  • Glycoside hydrolase, family 2 / Beta galactosidase small chain/ domain 5 / Domain of unknown function(DUF4981) / Glycosyl hydrolases family 2 acid/base catalyst. (pdbj.org)
  • Superposition of this complex with other beta-galactosidase complexes from several hydrolase families allowed the identification of residue Glu200 as the proton donor and residue Glu299 as the nucleophile involved in catalysis. (uzh.ch)
  • GLA, also named as Melibiase, Agalsidas and Alpha-galactosidase A, Belongs to the glycosyl hydrolase 27 family. (ptglab.com)
  • Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN). (wikipedia.org)
  • B-galactosidase can be used to track the efficiency of bacterial transformation with a recombinant plasmid in a process called Blue/White Color Screening. (wikipedia.org)
  • The new yeast strains prepared by using recombinant DNA methods produce more α-galactosidase than naturally occurring α-galactosidase producing yeast strains. (freepatentsonline.com)
  • Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. (abnova.com)
  • Human a -galactosidase A produced by recombinant DNA technology in cultured human cells. (drugfuture.com)
  • This Fab fragment recognizes wild-type E. coli beta-galactosidase. (abnova.com)
  • Galactosidases are enzymes (glycoside hydrolases) that catalyze the hydrolysis of galactosides into monosaccharides. (wikipedia.org)
  • If the galactoside is classified as an alpha-galactoside, the enzyme is called alpha-galactosidase, and is responsible for catalyzing the hydrolysis of substrates that contain α-galactosidic residues, such as glycosphingolipids or glycoproteins. (wikipedia.org)
  • The active site of β-galactosidase catalyzes the hydrolysis of its disaccharide substrate via "shallow" (nonproductive site) and "deep" (productive site) binding. (wikipedia.org)
  • The long-established ortho -nitrophenyl-β- d -galactopyranoside test relies on the hydrolysis of ortho -nitrophenyl-β- d -galactoside by β-galactosidase, releasing yellow ortho -nitrophenol ( 5 ). (asm.org)
  • Beta-galactosidases catalyze the hydrolysis of beta(1-3) and beta(1-4) galactosyl bonds in oligosaccharides as well as the inverse reaction of enzymatic condensation and transglycosylation. (uzh.ch)
  • Bowen, D. M. and Radin, N. S.: Cerebroside Galactosidase: A Method for Determination and a Comparison with Other Lysosomal Enzymes in Developing Rat Brain. (springer.com)
  • Saccharomyces fragilis is described as a homothallic, hemiascomycetous yeast and production of several enzymes among them beta-galactosidase (Llorente et al. (thefreedictionary.com)
  • The biological significance of A. niger beta-galactosidase multiplicity might be related to the observed differences in carbohydrate content, as suggested by recent reports on other microbial glycoprotein enzymes. (nih.gov)
  • In conclusion, high molecular weight enzymes, such as bacterial β-galactosidase, can be conjugated to BBB targeting antibodies for effective delivery across the BBB in vivo. (aspetjournals.org)
  • β-D-Galactosidase and β-D-glucuronidase are 2 marker enzymes used in the rapid detection of total coliforms and E. coli , respectively. (scielo.org.za)
  • In this study, the effect of 11 US EPA priority pollutant phenols (PPP) on these enzyme assays were investigated and it was shown that over- and under-estimation of β-D-galactosidase and β-D-glucuronidase activities may occur due to inhibition or activation of these enzymes in the presence of these phenolic compounds. (scielo.org.za)
  • We used Aspergillus oryzae (at temperatures of 10 and 55°C) and Kluyveromyces lactis (at temperatures of 10 and 37°C) β -galactosidases, both in 3, 6, and 9 U/mL concentrations. (hindawi.com)
  • beta-Galactosidase From Aspergillus Niger. (nih.gov)
  • Here we report a detailed analysis of the clearly different GOS profiles of the commercial β-galactosidases from Bacillus circulans, Kluyveromyces lactis and Aspergillus oryzae. (nih.gov)
  • The enzyme β-galactosidase from Aspergillus oryzae has been immobilized in aminated (MANAE)-agarose beads via glutaraldehyde chemistry using different strategies. (mdpi.com)
  • article{5a2616ea-8c6c-4969-8230-6273fb67eb97, abstract = {The anaerobic fermentation of deproteinized whey with beta-galactosidase coimmobilized with Saccharomyces cerevisiae in calcium alginate gel beads for the production of ethanol has been studied in a continuous horizontal packed bed reactor (HPBR). (lu.se)
  • For details on β-galactosidase see Molecular Playground/Beta-galactosidase . (proteopedia.org)
  • Beta-galactosidase, molecular model. (sciencephoto.com)
  • 5. The correlation between activity, stability and molecular form of beta-galactosidase is discussed. (biochemj.org)
  • The present studies use bacterial β-galactosidase, which has a molecular weight of 116,000 Da, as a model enzyme for delivery to mouse brain. (aspetjournals.org)
  • For details on α-galactosidase see Garman lab: Interconversion of lysosomal enzyme specificities . (proteopedia.org)
  • Fabry disease is an X-linked recessive inborn metabolic disorder in which a deficiency in lysosomal enzyme α-galactosidase A (Gal A) causes the systemic accumulation of globotriaosylceramide (Gb3). (springer.com)
  • Peanut pod-an inexpensive substrate for β-galactosidase production by Bacillus sp. (minervamedica.it)
  • Lack of alpha-galactosidase activity in leukocytes has been linked to Fabry Disease. (wikipedia.org)
  • When B-galactosidase is separated into 2 fragments, it has the unique property of regaining enzymatic activity upon the rejoining of the inactive fragments. (wikipedia.org)
  • When both fragments are present they would come together to restore the activity of B-galactosidase. (wikipedia.org)
  • The intensity (or optical density) of the yellow colour produced is a qualitative indicator or quantitative measure (with a colorimeter) of the β-galactosidase activity. (nuffieldfoundation.org)
  • Bluo-gal and X-gal are commonly used substrates for measuring enzymatic activity of beta-galactosidase in bacteria. (thermofisher.com)
  • Beta-galactosidase activity can be detected with the Luminescent Beta-Gal Detection Kit II. (clontech.com)
  • The Thermo Scientific Mammalian β-Galactosidase Assay Reagent is a component of the Mammalian β-Galactosidase Assay Kit, which provides a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity. (thermofisher.com)
  • The Assay Reagent and Kit comprise a simple and versatile assay system for measuring β-galactosidase activity. (thermofisher.com)
  • Mammalian cells grown on a plate may be lysed and tested for β-galactosidase activity by adding a single, all-in-one lysis-and-assay solution. (thermofisher.com)
  • Decreased alpha-galactosidase activity in urine and plasma. (ganfyd.org)
  • Under the optimum conditions, β-galactosidase was immobilized on the supporter obtained above and the enzyme activity bound per gram drier carrier could attained up to 77.33%, which was much higher than that bound on the carrier prepared by only using methanol aqueous solution as porogen. (hindawi.com)
  • More particularly, polypeptides of the present invention have been identified as glycosidases and/or α-galactosidases as a result of their enzymatic activity. (google.com)
  • Of note, A[sz] did not induce the senescence-associated beta-galactosidase activity in cultured neurons (data not shown), indicating that SA-[sz]-gal was not required for neuronal senescence in vitro , although it was a classic CS biomarker. (thefreedictionary.com)
  • Toussaint, "Protocols to detect senescence-associated beta-galactosidase (SA-[beta]gal) activity, a biomarker of senescent cells in culture and in vivo," Nature Protocols, vol. (thefreedictionary.com)
  • Bone marrow transplantation correcting beta-galactosidase activity does not influence neurological outcome in juvenile [G. (thefreedictionary.com)
  • The isolated enzyme forms accounted for 83%, 8%, and 9% of the total beta-galactosidase activity, respectively. (nih.gov)
  • These protocols are used to screen bacterial plates for beta-galactosidase activity using X-Gal (aka blue white screening). (openwetware.org)
  • There is a clear trend that galactosidase activity increases due to sonification, improving the viability of probiotics. (thefreelibrary.com)
  • AnaSpec is pleased to announce the release of 3 new assay kits for measuring -galactosidase activity the SensoLyte MUG -Galactosidase Assay Kit (Ex/Em= 360/460 nm), SensoLyte FDG -Galactosidase Assay Kit (Ex/Em= 490/520 nm), and SensoLyte ONPG -Galactosidase Assay Kit (Abs=420 nm). (hum-molgen.org)
  • Penicillium simplicissimum , a strain isolated from soil, when grown in semi-solid medium showed good productivity of b -galactosidase with galactosyltransferase activity. (scielo.br)
  • The unconjugated β-galactosidase or the β-galactosidase-8D3 conjugate was injected intravenously in adult mice, and enzyme activity was measured at 1 and 4 h in brain and peripheral organs (liver, spleen, kidney, and heart). (aspetjournals.org)
  • a In the initial phase, only total α-galactosidase activity was measured. (asnjournals.org)
  • Beta-Galactosidase (Penicillium) may be available in the countries listed below. (drugs.com)
  • Crystal structures of beta-galactosidase from Penicillium sp. (ebi.ac.uk)
  • This work describes the hydrolytic and transgalactosylase activities of a semi-purified b -galactosidase produced by a strain of Penicillium simplicissimum isolated from soil. (scielo.br)
  • The Beta-Galactosidase Staining Kit is a complete system for colorimetric detection of beta-galactosidase expression in mammalian cells. (clontech.com)
  • We describe the synthesis of a new substrate for the detection of β-galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-β- d -galactopyranoside (X-Gal) and cyclohexenoesculetinβ- d -galactoside (CHE-Gal). (asm.org)
  • We conclude that PNB-Gal is an effective substrate for the detection of β-galactosidase. (asm.org)
  • We describe the synthesis of a new chromogenic substrate for the detection of β-galactosidase, p -naphtholbenzein-β- d -galactopyranoside (PNB-Gal) (Fig. 1 ). (asm.org)
  • The effectiveness of this substrate was evaluated in direct comparison to cyclohexenoesculetin-β- d -galactoside (CHE-Gal) and X-Gal for the detection of β-galactosidase within the Enterobacteriaceae and other gram-negative species. (asm.org)
  • ABBOO, Sagaran y PLETSCHKE, Brett I . Effect of phenolic compounds on the rapid direct enzymatic detection of β -D-galactosidase and β -D-glucuronidase . (scielo.org.za)
  • Synthetic peptide within Human GLB1/Beta-galactosidase aa 315-365 conjugated to keyhole limpet haemocyanin. (abcam.com)
  • Global Markets Direct's, 'Alpha-Galactosidase A (Alpha-D-Galactosidase A or Alpha-D-Galactoside Galactohydrolase or Melibiase or EC 3.2.1.22) - Pipeline Review, H1 2016', provides in depth analysis on Alpha-Galactosidase A (Alpha-D-Galactosidase A or Alpha-D-Galactoside Galactohydrolase or Melibiase or EC 3.2.1.22) targeted pipeline therapeutics. (marketresearch.com)
  • The report provides comprehensive information on the Alpha-Galactosidase A (Alpha-D-Galactosidase A or Alpha-D-Galactoside Galactohydrolase or Melibiase or EC 3.2.1.22), targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (marketresearch.com)
  • Additionally, the report provides an overview of key players involved in Alpha-Galactosidase A (Alpha-D-Galactosidase A or Alpha-D-Galactoside Galactohydrolase or Melibiase or EC 3.2.1.22) targeted therapeutics development and features dormant and discontinued projects. (marketresearch.com)
  • Two fold dilutions of β1-3 Galactosidase are incubated with 1 nmol AMC-labeled substrate in 1X GlycoBuffer 1, supplemented with 100 µg/ml BSA, in a 10 µl reaction. (neb.com)
  • CONCLUSION: Our finding showed that combination of peanut pod with wheat and rice straw could be used as suitable and low-cost substrate for β-galactosidase production. (minervamedica.it)
  • It shows reactivity to ß-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of E. coli. (mpbio.com)
  • The ELISA with anti-AMD immunoglobulin G fraction as a solid phase and 7-aminoactinomycin β- d -galactosidase conjugate was specific to AMD as well as 7AMD and showed 30% cross-reaction with actinomycin V, while no cross-reactivity was seen with drugs commonly used with AMD in combination chemotherapy for cancer treatment. (aacrjournals.org)
  • 66586-1-Ig targets Beta galactosidase in WB, IHC, IF, ELISA applications and shows reactivity with Human samples. (ptglab.com)
  • A series of plasmids encoding hybrid proteins comprising various lengths of the NH2-terminal region of colicin N coupled to almost complete beta-galactosidase or alkaline phosphatase polypeptides was constructed by transposon mutagenesis of ColN plasmid derivatives. (epfl.ch)
  • Large quantities of the hybrid proteins accumulated in the cytoplasm (beta-galactosidase) or particulate fractions (alkaline phosphatase). (epfl.ch)
  • Computer model showing the 4-chain-structure of bacterial beta-galactosidase. (sciencephoto.com)
  • Bacterial β-galactosidase (116 kDa) was conjugated to the rat 8D3 mAb to the rat transferrin receptor (TfR) via a streptavidin-biotin linkage. (aspetjournals.org)
  • beta-galactosidase belongs to family 35 of glycosyl hydrolases (GHF-35). (uzh.ch)
  • α-Galactosidase (Agal) hydrolyzes the terminal α-galactosyl moiety from glycoproteins and glycolipids [1] . (proteopedia.org)
  • The beta-galactosidase assay is commonly used as a reporter, to monitor transfection efficiency, and for promoter/enhancer studies. (clontech.com)
  • The β-Galactosidase Staining Kit is an easy-to-use and efficient method to determine the percentage of cells expressing lac Z following transient or stable transfection of plasmids that contain lac Z. (activemotif.com)
  • The 1,023 amino acids of E. coli β-galactosidase were accurately sequenced in 1983, and its structure determined twenty-four years later in 1994. (wikipedia.org)
  • A mutant with a deletion of everything past residue 60 (i.e. it expresses only the first 60 N-terminal amino acids) does not produce any active β-galactosidase. (openwetware.org)
  • Similarly a change of the vacuole-targeting sequence QKKLD, amino acids 80-84, to QKNSD did not affect significantly the course of inactivation of β-galactosidase. (csic.es)
  • The anaerobic fermentation of deproteinized whey with beta-galactosidase coimmobilized with Saccharomyces cerevisiae in calcium alginate gel beads for the production of ethanol has been studied in a continuous horizontal packed bed reactor (HPBR). (lu.se)
  • Figure 1: Staining of HeLa cells using the β-Galactosidase Staining Kit. (activemotif.com)
  • Two hours post-delivery, cells were fixed and stained using the β-Galactosidase Staining Kit. (activemotif.com)
  • pCMV-LacZ is a constitutive mammalian reporter vector which expresses extremely high levels of beta-galactosidase from the human cytomegalovirus immediate early promoter ( P CMV IE ) and can be used as a reference or control plasmid. (clontech.com)
  • Beta-galactosidase has many homologues based on similar sequences. (wikipedia.org)
  • is beta-galactosidase a glycoprotein? (bio.net)
  • I have been told that beta-galactosidase may be suitable. (bio.net)
  • 17 amino acid peptide from near the N terminus of beta Galactosidase from E. coli (NP_752394). (abcam.com)
  • The Luminescent Beta-Galactosidase Reporter System 3 includes the chemiluminescent beta-galactosidase assay reagents plus the pBeta-Gal-Basic and pBeta-Gal-Control vectors. (clontech.com)
  • beta-Galactosidase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. (harvard.edu)
  • This graph shows the total number of publications written about "beta-Galactosidase" by people in Harvard Catalyst Profiles by year, and whether "beta-Galactosidase" was a major or minor topic of these publication. (harvard.edu)
  • Below are the most recent publications written about "beta-Galactosidase" by people in Profiles. (harvard.edu)
  • Lac repressor can be fused to beta‐galactosidase. (currentprotocols.com)
  • ClinicalTrials.gov lists trials that are related to Beta-galactosidase-1 deficiency. (nih.gov)
  • beta Galactosidase (DC3 10D6). (abnova.com)
  • galactosidase in an aqueous medium to form an admixture and maintaining the admixture so formed under biological reaction conditions for a time period sufficient toform said galactosyl.beta.1,3glycal disaccharide. (patentgenius.com)
  • galactosidase in an aqueous medium having a pH value of about 6.0 to about 8.0 atambient room temperature to form a reaction mixture, and maintaining the reaction mixture for a time period sufficient for Gal.beta.1,3(6-O-Ac)Glucal to form. (patentgenius.com)
  • 1. A method is described for following continuously the action of beta-galactosidase on 4-methylumbelliferyl beta-D-galactoside at pH 4.5, in which 4-methylumbelliferone production is measured at fluorescence excitation and emission wavelengths of 324 and 444nm respectively. (biochemj.org)
  • 2. Initial-rate studies show that the presence of salt activates beta-galactosidase up to 100 mM, but is inhibitory above that concentration. (biochemj.org)
  • The implications of this finding for the assay of beta-galactosidase under physiological conditions for prenatal diagnosis are discussed. (biochemj.org)
  • from of beta-galactosidase is presented. (biochemj.org)
  • B-factor corrected reconstruction of PETG-bound beta-galactosidase. (pdbj.org)
  • After 3 days, 0.1 to 1% of the plaques became blue in the presence of beta-galactosidase indicator. (asm.org)
  • Five distinct domains which comprise the structure are assembled in a way previously unobserved for beta-galactosidases. (uzh.ch)
  • beta-galactosidase is a glycoprotein containing seven N-linked oligosaccharide chains and is the only structure of a glycosylated beta-galactosidase described to date. (uzh.ch)
  • IPTG induces production of ß-galactosidase by binding and inhibiting the lac repressor. (acris-antibodies.com)
  • b -Galactosidase or b -D-galactoside-galactohydrolase (EC. (scielo.br)
  • InvivoGen provides several isotype control antibodies targeting E. coli β-galactosidase (β-Gal). (invivogen.com)