A nucleoside diphosphate sugar formed from GDPmannose, which provides fucose for lipopolysaccharides of bacterial cell walls, and for blood group substances and other glycoproteins.
Enzymes catalyzing the transfer of fucose from a nucleoside diphosphate fucose to an acceptor molecule which is frequently another carbohydrate, a glycoprotein, or a glycolipid molecule. Elevated activity of some fucosyltransferases in human serum may serve as an indicator of malignancy. The class includes EC 2.4.1.65; EC 2.4.1.68; EC 2.4.1.69; EC 2.4.1.89.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.
The characteristic 3-dimensional shape of a carbohydrate.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
The N-acetyl derivative of glucosamine.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
A class of Echinodermata characterized by long, slender bodies.
A group of dominantly and independently inherited antigens associated with the ABO blood factors. They are glycolipids present in plasma and secretions that may adhere to the erythrocytes. The phenotype Le(b) is the result of the interaction of the Le gene Le(a) with the genes for the ABO blood groups.
Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)
SUGARS containing an amino group. GLYCOSYLATION of other compounds with these amino sugars results in AMINOGLYCOSIDES.
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3.
An amidohydrolase that removes intact asparagine-linked oligosaccharide chains from glycoproteins. It requires the presence of more than two amino-acid residues in the substrate for activity. This enzyme was previously listed as EC 3.2.2.18.
A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
Sets of cell surface antigens located on BLOOD CELLS. They are usually membrane GLYCOPROTEINS or GLYCOLIPIDS that are antigenically distinguished by their carbohydrate moieties.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
Antibodies produced by a single clone of cells.
Submicron-sized fibers with diameters typically between 50 and 500 nanometers. The very small dimension of these fibers can generate a high surface area to volume ratio, which makes them potential candidates for various biomedical and other applications.
A form of antibodies consisting only of the variable regions of the heavy and light chains (FV FRAGMENTS), connected by a small linker peptide. They are less immunogenic than complete immunoglobulin and thus have potential therapeutic use.
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Antibodies, often monoclonal, in which the two antigen-binding sites are specific for separate ANTIGENIC DETERMINANTS. They are artificial antibodies produced by chemical crosslinking, fusion of HYBRIDOMA cells, or by molecular genetic techniques. They function as the main mediators of targeted cellular cytotoxicity and have been shown to be efficient in the targeting of drugs, toxins, radiolabeled haptens, and effector cells to diseased tissue, primarily tumors.
Wnt proteins are a large family of secreted glycoproteins that play essential roles in EMBRYONIC AND FETAL DEVELOPMENT, and tissue maintenance. They bind to FRIZZLED RECEPTORS and act as PARACRINE PROTEIN FACTORS to initiate a variety of SIGNAL TRANSDUCTION PATHWAYS. The canonical Wnt signaling pathway stabilizes the transcriptional coactivator BETA CATENIN.
A complex signaling pathway whose name is derived from the DROSOPHILA Wg gene, which when mutated results in the wingless phenotype, and the vertebrate INT gene, which is located near integration sites of MOUSE MAMMARY TUMOR VIRUS. The signaling pathway is initiated by the binding of WNT PROTEINS to cells surface WNT RECEPTORS which interact with the AXIN SIGNALING COMPLEX and an array of second messengers that influence the actions of BETA CATENIN.
Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
A Wnt protein subtype that plays a role in cell-cell signaling during EMBRYONIC DEVELOPMENT and the morphogenesis of the developing NEURAL TUBE.
A Wnt protein subtype that plays a role in cell-cell signaling during EMBRYONIC DEVELOPMENT and the morphogenesis of the developing NEURAL TUBE. Defects in Wnt3 protein are associated with autosomal recessive tetra-AMELIA in humans.

Glycopeptides from the surgace of human neuroblastoma cells. (1/1136)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Gangliosides of human kidney. (2/1136)

Five gangliosides isolated from human kidney have been characterized. The two main fractions were shown to be typical extraneural gangliosides in having lactose as their neutral carbohydrate moiety. Their structures were identified as: AcNeu(alpha2-3)Gal(beta1-4)Glc(beta1-1)Cer and AcNeu(alpha2-8)AcNeu(alpha2-3)Gal(beta1-4)Glc(beta1-1)Cer. The two main hexosamine-containing gangliosides are structurally related to human blood group substances of glycosphingolipid nature. The following structures are postulated: AcNeu(alpha2-3)Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)Glc(beta1-1)Cer and AcNeu(alpha2-3)Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-3)Gal(beta1-4)Glc(beta1-1) Cer. The third hexosamine-containing ganglioside belongs to a different series of glycolipids and was shown to have the structure of a major ganglioside of human brain: AcNeu(alpha2-3)Gal(beta1-3)GalNAc(beta1-4)[AcNeu(alpha2-3)]Gal(beta1-4)Glc(beta1- 1)Cer. The fatty acid structure of different gangliosides was shown to resemble that of neutral glycolipids of human kidney with the nonhydroxy acids C16:0, C22:0, and C24:0 as major components.  (+info)

The nolL gene from Rhizobium etli determines nodulation efficiency by mediating the acetylation of the fucosyl residue in the nodulation factor. (3/1136)

The nodulation factors (Nod factors) of Rhizobium etli and R. loti carry a 4-O-acetyl-L-fucosyl group at the reducing end. It has been claimed, based on sequence analysis, that NolL from R. loti participates in the 4-O-acetylation of the fucosyl residue of the Nod factors, as an acetyl-transferase (D. B. Scott, C. A. Young, J. M. Collins-Emerson, E. A. Terzaghi, E. S. Rockman, P. A. Lewis, and C. E. Pankhurst. Mol. Plant-Microbe Interact. 9:187-197, 1996). Further support for this hypothesis was obtained by studying the production of Nod factors in an R. etli nolL::Km mutant. Chromatographic and mass spectrometry analysis of the Nod factors produced by this strain showed that they lack the acetyl-fucosyl substituent, having a fucosyl group instead. Acetyl-fucosylation was restored upon complementation with a wild-type nolL gene. These results indicate that the nolL gene determines 4-O-acetylation of the fucosyl residue in Nod factors. Analysis of the predicted NolL polypeptide suggests a transmembranal location and that it belongs to the family of integral membrane transacylases (J. M. Slauch, A. A. Lee, M. J. Mahan, and J. J. Mekalanos. J. Bacteriol. 178:5904-5909, 1996). NolL from R. loti was also proposed to function as a transporter; our results show that NolL does not determine a differential secretion of Nod factors from the cell. We also performed plant assays that indicate that acetylation of the fucose conditions efficient nodulation by R. etli of some Phaseolus vulgaris cultivars, as well as of an alternate host (Vigna umbellata).  (+info)

Effect of leukocytes on corneal cellular proliferation and wound healing. (4/1136)

PURPOSE: To establish whether fucoidin, by blocking the adhesion of leukocytes on the limbal vascular endothelium, prevents extravasation of the cells from the blood stream into the limbal stroma and the wounded area after corneal injury. Successful leukocyte blocking enabled investigation of the influence of leukocytes on corneal cellular proliferation after corneal wounding. METHODS: Thirty-two New Zealand White rabbits were used. Photorefractive keratectomy (PRK) and a standardized alkali corneal wound were used as models in two sets of experiments. In half of the injured rabbits fucoidin was used to prevent leukocytes from leaving the local vessels. The efficiency of the blocking technique was evaluated by counting the number of leukocytes in the limbal and wounded corneal areas. Proliferating cell nuclear antigen (PCNA) was used as a marker for proliferative activity. RESULTS: The infiltration of leukocytes into the limbus and the cornea after PRK and alkali injuries can be blocked by fucoidin. The healing rate of corneal epithelium after alkali burn was retarded in the absence of leukocytes. PCNA expression was enhanced in the presence of leukocytes. Fucoidin per se had no influence on corneal cell proliferation and wound healing. CONCLUSIONS: Polymorphonuclear leukocytes (PMNs) can be prevented from entering the cornea in vivo by fucoidin after PRK and after alkali burn. The corneal epithelial healing rate is delayed in the absence of PMNs in vivo, and PCNA expression increases in the presence of leukocytes.  (+info)

Enzymatic synthesis of alpha3'sialylated and multiply alpha3fucosylated biantennary polylactosamines. A bivalent [sialyl diLex]-saccharide inhibited lymphocyte-endothelium adhesion organ-selectively. (5/1136)

Multifucosylated sialo-polylactosamines are known to be high affinity ligands for E-selectin. PSGL-1, the physiological ligand of P-selectin, is decorated in HL-60 cells by a sialylated and triply fucosylated polylactosamine that is believed to be of functional importance. Mimicking some of these saccharide structures, we have synthesized enzymatically a bivalent [sialyl diLex]-glycan, Neu5Acalpha2-3'Lexbeta1-3'Lexbeta1-3'(Neu5Acalpha2-3'Lexbeta1-3Lexbe ta1-6')LN [where Neu5Ac is N-acetylneuraminic acid, Lex is the trisaccharide Galbeta1-4(Fucalpha1-3)GlcNAc and LN is the disaccharide Galbeta1-4GlcNAc]. Several structurally related, novel polylactosamine glycans were also constructed. The inhibitory effects of these glycans on two L-selectin-dependent, lymphocyte-to-endothelium adhesion processes of rats were analysed in ex-vivo Stamper-Woodruff binding assays. The IC50 value of the bivalent [sialyl diLex]-glycan at lymph node high endothelium was 50 nm, but at the capillaries of rejecting cardiac allografts it was only 5 nm. At both adhesion sites, the inhibition was completely dependent on the presence of fucose units on the sialylated LN units of the inhibitor saccharide. These data show that the bivalent [sialyl diLex]-glycan is a high affinity ligand for L-selectin, and may reduce extravasation of lymphocytes at sites of inflammation in vivo without severely endangering the normal recirculation of lymphocytes via lymph nodes.  (+info)

Isolation and characterization of a novel fucoganglioside of human erythrocyte membranes. (6/1136)

A slow migrating monosialosyl ganglioside with blood group H activity was isolated from human erythrocyte membranes and identified as monosialosylceramide decasaccharide. Its structure was determined by degradation with exo- and endoglycosidases, by mass spectrometric characterization of a nonasaccharide liberated by endo-beta-galactosidase, and by methylation analysis and mass spectrometry of permethylated glycolipids before and after enzymatic degradation. The ganglioside contains two oligosaccharide chains branched through GlcNAcbeta1 leads to 6(GlcNAcbeta1 leads to 3) Gal structure, and the terminal Gal of the 1 leads to 6-linked oligosaccharide was fucosylated, whereas that of the 1 leads to 3-linked oligosaccharide was sialosylated as seen below.  (+info)

Analysis of the expression and enzymatic properties of alpha1-->3fucosyltransferase from human lung carcinoma NCI-H69 and PC9 cells. (7/1136)

An analysis of alpha1-->3fucosyltransferase expression and enzyme properties has been conducted in human lung carcinoma NCI-H69 and PC9 cells. The results indicate that multiple forms of alpha1-->3 fucosyltransferase are found in these cells. RT-PCR experiments using total RNA from NCI-H69 and PC9 cells amplified transcripts for three of these enzymes, FucT-IV, -VI, and -VII. Fucose transfer into glycolipid acceptors mediated by truncated chimeric and full length recombinant FucT-IV and -VI enzymes was examined. Both enzymes were found to be type 2 chain specific, but only FucT-VI efficiently transferred fucose to both neutral and sialylated acceptors. A truncated recombinant form of FucT-VI was capable of fucose transfer to the internal Glc residue of a variety of glycolipid acceptors. This property was not observed with the recombinant full length enzyme, suggesting the N-terminal portion of the protein, composed of the intracellular domain, transmembrane domain, and a part of the stem region, is involved in interactions with glycolipid acceptors. Using taurodeoxycholate as the detergent, the distribution of initial fucose transfer into nLc6catalyzed by recombinant full length enzyme indicated 34% of the mono-fucosyl product was fucosylated at the III-GlcNAc and 66% at the V-GlcNAc for FucT-IV, and almost all of the FucT-VI mono-fucosyl product was III-GlcNAc fucosylated. Similar experiments with VI2NeuAcnLc6as the acceptor resulted in predominantly III-GlcNAc monofucosylation, although detectable V-GlcNAc monofucosylation was obtained with FucT-VI. When the cationic detergent G-3634-A was used, substantially greater initial transfer into the V-GlcNAc of both neutral and sialylated acceptors with FucT-VI was observed. Using nonsialylated acceptors, total alpha1-->3 fucosyltransferase activity in NCI-H69 cells was analyzed and found to be diminished 25-30% by exposure to 30 mM NEM, which can be attributed to FucT-VI inactivation. The remaining 70-75% of NEM-resistant activity is attributed to FucT-IV, an NEM-resistant enzyme form capable of fucosylating nonsialylated acceptors. These results suggest that multiple forms of alpha1-->3fucosyltransferase are expressed in NCI-H69 and PC9 cells, which may account for the observed properties of enzyme derived from these cell lines.  (+info)

The O-linked fucose glycosylation pathway: identification and characterization of a uridine diphosphoglucose: fucose-beta1,3-glucosyltransferase activity from Chinese hamster ovary cells. (8/1136)

O-Linked fucose is an unusual carbohydrate modification in which fucose is linked directly to the hydroxyl groups of serines or threonines. It has been found on the epidermal growth factor-like modules of several secreted proteins involved in blood coagulation and fibrinolysis. We have recently reported the existence of an elongated form of O-linked fucose in Chinese hamster ovary cells consisting of a glucose linked to the 3'-hydroxyl of fucose (Glcbeta1,3Fuc- O-Ser/Thr). This structure is highly unusual for two reasons. First, in mammalian systems fucose is usually a terminal modification of N - and O-linked oligosaccharides. Here the fucose is internal. Secondly, terminal beta-linked glucose is extremely rare on mammalian glycoconjugates. Thus, the Glcbeta1,3Fuc structure is a very unique mammalian carbohydrate structure. Here we report the identification and initial characterization of a novel enzyme activity capable of forming this unique linkage: UDP-glucose: O-linked fucose beta1,3 glucosyltransferase. The enzyme utilizes UDP-glucose as the high energy donor and transfers glucose to alpha-linked fucose residues. The activity is linearly dependent on time, enzyme, and substrate concentrations and is enhanced in the presence of manganese ions. Activity is present in extracts of cultured cells from a variety of species (hamster, human, mouse, rat, chicken) and is enriched in brain and spleen of a normal adult rat. Thus, while this glycosyltransferase appears to be widespread in biology, it forms a very unique linkage, and it represents the first mammalian enzyme identified capable of elongating fucose.  (+info)

Recombinant Aleuria aurantia lectin is produced in E.coli and has an amino acid sequence identical to native Aleuria aurantia lectin. AAL is a dimeric lectin with two identical subunits of approximately 36 kDa. Each subunit has five carbohydrate-binding sites. The lectin recognizes and binds specifically to fucose and terminal fucose residues on complex oligo saccharides and glycoconjugates. rAAL has binding affinity for fucose in all binding positions (alpha1-2, alpha1-3, alpha1-4 and alpha1-6) and in contrast to AAL purified from natural sources, rAAL is not contaminated with free fucose yielding higher affinity towards fucosylated oligosaccharides than native AAL. Recombinant AAL hemagglutinates erythrocytes irrespective of blood type (A, B and 0) at the same titers as AAL isolated from natural sources. AAL has been widely used for analysis and preparation of oligosaccharides and glycoproteins. Diagnostic applications include analysis of disease-associated glycosylation on plasma proteins.
Purified Native Aleuria Aurantia Lectin Protein from Creative Biomart. Native Aleuria Aurantia Lectin Protein can be used for research.
Antibody-dependent cellular cytotoxicity (ADCC) has recently been identified as one of the critical mechanisms underlying the clinical efficacy of therapeutic antibodies, especially anticancer antibodies. Therapeutic antibodies fully lacking the core fucose of the Fc oligosaccharides have been found to exhibit much higher ADCC in humans than their fucosylated counterparts. However, data which show how fully non-fucosylated antibodies achieve such a high ADCC in human whole blood have not yet been disclosed. The precise mechanisms responsible for the high ADCC mediated by fully non-fucosylated therapeutic antibodies, even in the presence of human plasma, should be explained based on direct evidence of non-fucosylated antibody action in human blood. Using a human ex vivo B-cell depletion assay with non-fucosylated and fucosylated anti-CD20 IgG1s rituximab, we monitored the binding of the therapeutic agents both to antigens on target cells (target side interaction) and to leukocyte receptors (FcγR) on
TY - JOUR. T1 - The binding of fucose-containing glycoproteins by hepatic lectins. Re-examination of the clearance from blood and the binding to membrane receptors and pure lectins. AU - Lehrman, M. A.. AU - Pizzo, S. V.. AU - Imber, M. J.. AU - Hill, R. L.. PY - 1986. Y1 - 1986. N2 - The nature of the hepatic receptors that bind glycoproteins through fucose at the non-reducing termini of oligosaccharides in glycoproteins has been examined by three different approaches. First, the clearance from blood of intravenously injected glycoproteins was examined in mice with the aid of neoglycoproteins of bovine serum albumin (BSA). The clearance of fucosyl-BSA was rapid and was not strongly inhibited by glycoproteins that inhibit clearance mediated by the galactose or the mannose/N-acetylglucosamine receptors of liver. The clearance of Fucα1,3(Galβ1,4)GlcNAc-BSA (where Fuc is fucose) was inhibited weakly by either Fuc-BSA or Galβ1,4GlcNAc-BSA but strongly by a mixture of the two neoglycoproteins, ...
A mammalian N-acetylglucosamine (GlcNAc) transferase I (GnT I)-independent fucosylation pathway is revealed by the use of matrix-assisted laser desorption/ionization (MALDI) and negative-ion nano-electrospray ionization (ESI) mass spectrometry of N-linked glycans from natively folded recombinant glycoproteins, expressed in both human embryonic kidney (HEK) 293S and Chinese hamster ovary (CHO) Lec3.2.8.1 cells deficient in GnT I activity. The biosynthesis of core fucosylated Man5GlcNAc2 glycans was enhanced in CHO Lec3.2.8.1 cells by the alpha-glucosidase inhibitor, N-butyldeoxynojirimycin (NB-DNJ), leading to the increase in core fucosylated Man5GlcNAc2 glycans and the biosynthesis of a novel core fucosylated monoglucosylated oligomannose glycan, Glc1Man7GlcNAc2Fuc. Furthermore, no fucosylated Man9GlcNAc2 glycans were detected following inhibition of alpha-mannosidase I with kifunensine. Thus, core fucosylation is prevented by the presence of terminal alpha1-2 mannoses on the 6-antennae but not the 3
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The objective of this investigation was primarily to establish that the apparent increase in serum fucose levels in patients with malignant tumors is real and to obtain data as to its source. Carbohydrate residues (L-fucose, D-mannose, D-galactose) were estimated by borate ion-exchange chromatography after mild acid hydrolysis of serum proteins. Data are presented which confirm reported increases in levels of serum fucose in certain malignancies and indicate that these increases may be attributed to quantitative alterations in specific serum glycoproteins observed in the glycoprotein profile associated with cancer. Values for serum fucose levels in pathological and nonpathological sera obtained by various analytical methods are presented and discussed. (Author)
D-Fucose,6-Deoxy-D-galactose,D-galactomethylose,rhodeose,Pentaacetate,D-Fucose Pentaacetate,Oxime,D-Fucose Oxime,Phenylhydrazone,D-Fucose Phenylhydrazone,Phenylosazone,D-Fucose Phenylosazone
Glycoproteins were metabolically labeled with 3H-fucose in cultured RPE cells from RCS rdy-p+ and Long Evans rats. 3H-labeled glycoproteins associated with a plasma membrane-enriched subcellular fraction were separated by two-dimensional gel electrophoresis. Relative incorporation of 3H-fucose into high molecular weight cell surface glycoproteins (Mr of 128,000-183,000) was measured by quantitative autoradiography and densitometry. The results of these experiments show that 3H-fucose incorporation into four glycoproteins (Mr of 183,000, 175,000, 164,000 and 156,000) was reduced by 30-50% in the dystrophic RPE as compared to the normal cells. This reduction was not due to an absence of the protein core of glycoproteins on the dystrophic RPE cell surface; when RPE cells were labeled with 3H-leucine prior to analysis, no reduction in label was found in the dystrophic RPE as compared to normal. Therefore, the results of this study suggest that the RCS rat RPE processes the oligosaccharide portion of ...
We found altered N-linked glycan profiles in PMP tissue specimens when compared with appendix controls, and increased fucosylation was an especially prominent feature. In addition to overall fucosylation, complex fucosylation (multifucosylation) was increased in PMP samples. Previously, altered fucosylation has been associated with aggressiveness of colorectal cancer (35, 36), which may depend on increased EGFR-mediated signaling (37) and modulation of adhesion molecule function (38, 39). In addition, core fucosylation was recently shown to drive melanoma metastasis by preventing the proteolytic cleavage of adhesion molecule L1CAM (40).. Next we studied fucosylation-related gene expression in PMP tissue specimens compared with control appendices using DNA microarray (25). In line with glycan profiles, we found increased expression of the core fucosylation performing enzyme FUT8, as well as three enzymes needed in the biosynthesis of its donor substrate GDP-fucose. With immunohistochemistry we ...
anti-fucose, fucose, glycosylation, Anti-fucose antibody, AS07 268anti-fucose, fucose, glycosylation, Anti-fucose antibodyThis antibody specifically cross-reacts against the core fucose residues bound to the protein N-glycans in alpha 1,3. This residue is
24. Boeynaems, S., Bogaert, E., Kovacs, D., Konijnenberg, A., Timmerman, E., Volkov, A., Guharoy, M., De Decker, M., Jaspers, T., Ryan, V.H., Janke, A.M., Baatsen, P., Vercruysse, T., Kolaitis, R.M., Daelemans, D., Taylor, J.P., Kedersha, N., Anderson, P., Impens, F., Sobott, F., Schymkowitz, J., Rousseau, F., Fawzi, N.L., Robberecht, W., Van Damme, P., Tompa, P. & Van Den Bosch, L. Phase Separation of C9orf72 Dipeptide Repeats Perturbs Stress Granule Dynamics. Mol. Cell 65, 1044-1055 (2017). PDF(3.6 MB). 23. Antonik, P.M.*, Volkov, A.N.*, Broder, U.N., Lo Re, D., van Nuland, N.A.J. & Crowley, P.B. Anomer-specific recognition and dynamics in a fucose-binding lectin. Biochemistry 55, 1195-203 (2016). *contributed equally. 22. Volkov, A.N. Structure and function of transient encounters of redox proteins. Acc. Chem. Res. 48, 3036-43 (2015). PDF(2.9 MB). 21. Van de Water, K., Sterckx, Y.G.J. & Volkov, A.N. The low-affinity complex of cytochrome c and its peroxidase. Nat. Commun. 6, 7073 (2015). ...
29. Hubin, E., Cioffi, F., Rozenski, J., van Nuland, N.A.J. & Broersen, K. (2016) Characterization of insulin-degrading enzyme-mediated cleavage of Aß in distinct aggregation states. Biochim. Biophys, Acta 1860, 1281-90.. 28. Antonik, P.M., Volkov, A.N., Broder, U.N., Lo Re, D., van Nuland, N.A.J. & Crowley, P.B. (2016) Anomer-specific recognition and dynamics in a fucose-binding lectin. Biochemistry 55, 1195-203.. 27. Moroz, Y.S., Dunston, T.T., Makhlynets, O.V., Moroz, O.V., Wu, Y., Yoon, J.H., Olsen, A.B., McLaughlin, J.M., Mack, K.L., Gosavi, P.M., van Nuland, N.A.J. & Korendovych, I.V. (2015) New tricks for old proteins: single mutations in a nonenzymatic protein give rise to various enzymatic activities. J. Am. Chem. Soc. 137, 14905-11.. 26. Huculeci, R., Garcia-Pino, A., Buts, L., Lenaerts, T. & van Nuland, N.A.J. (2015) Structural insights into the intertwined dimer of fyn SH2. Protein Sci. 24, 1964-78.. 25. Van de Water, K., Sterckx, Y.G.J. & Volkov, A.N. (2015) The low-affinity ...
Boc Sciences offers cas 1,2,4,6-Tetra-O-acetyl-3-deoxy-D-galactose in bulk,please inquire us to get a quote for 1,2,4,6-Tetra-O-acetyl-3-deoxy-D-galactose.
Albuminuria is an independent risk factor for renal interstitial fibrosis (RIF). Glomerular-filtered albumin in endocytic and non-endocytic pathways may injure proximal tubular epithelial cells (PTECs) via megalin and TGFβRII, respectively. Since megalin and TGFβRII are both modified by post-translational core fucosylation, which plays a critical role in RIF. Thus, we sought to identify whether core fucosylation is a potential target for reducing albumin-induced injury to PTECs. We constructed a human PTEC-derived cell line (HK-2 cells) and established an in vitro model of bovine serum albumin (BSA) injury ...
It has been hypothesized that ADCC is a general mechanism of action for many therapeutic antibodies (32). However, it remains unclear whether ADCC or the engagement of Fcγ receptors significantly contribute to trastuzumabs antitumor effects in patients with breast cancer. After the initial in vitro findings, Ravetch and colleagues showed the in vivo importance of FcγR-mediated activity of trastuzumab (13). Notably, these studies did not address the role of FcγR interaction in models with established tumors. Instead, treatments were simultaneous with cell inoculation and thus the results might portray the role of effector cell functions on tumor implantation rather than its effect on solid tumor mass. Our findings confirm the results of Clynes and colleagues and extend them to the treatment of pre-established solid tumors.. The ability to increase in vitro ADCC by antibody engineering is widely established (33, 34). However, to our knowledge, it has not been previously shown that the increase ...
Sigma-Aldrich offers abstracts and full-text articles by [Ayako Kurimoto, Shinobu Kitazume, Yasuhiko Kizuka, Kazuki Nakajima, Ritsuko Oka, Reiko Fujinawa, Hiroaki Korekane, Yoshiki Yamaguchi, Yoshinao Wada, Naoyuki Taniguchi].
Clearance of various bacteria isolated from portal and systemic blood of mice was evaluated and compared. All portal blood strains, including Escherichia coli and enterococci were eliminated more rapidly from the circulation than were strains isolated from systemic blood, including Pseudomonas aeruginosa. With mannose-type lectin, mannose or fucose residues that mediated lectinophagocytosis were detected on the surfaces of most portal strains by agglutination tests. Blood clearance of Esch. coli H21 was inhibited by prior injection of mannose into mice, suggesting that the clearance of this strain was mediated by mannose-type lectin on the surface of tissue macrophages. However, no inhibition of clearance of any other strains was observed by the injection of mannose, galactose, or fucose into mice, nor by pre-incubation of bacteria with mannose. Blood clearance of some portal strains was significantly faster in CBA/J mice than in CBA/N mice with B cell immune deficiency, indicating that immunoglobulin
The monosaccharide L-Fucose is a key component of important glycan structures found on extracellular mucoproteins and glycoproteins, in breast milk, and in certain blood-group determinants. L-Fucose is essential for human development and health.
The Fast Glycaneering process reportedly allows customized N-glycosylation to meet target profiles, specifically antibodies that harbor afucosylated humanized N-glycans (with increased ADCC) or humanized N-glycans.
Barbarahaworthattard.com is a website that fucose on sharing news and information related to computers to all the people who interested in or need it. We mainly write about guides to fix various computer errors/problems such as EXE/DLL errors, System slow down problem, computer freeze / crash, etc, you can also find other useful information like computer purchase guide, computer hardware recommendation, computer maintenance tips and more.. You may click here to visit the homepage of www.barbarahaworthattard.com to check out what kind of information you interested in or need. If you have any question or suggestion about our site or articles, feel free to leave a message to us.. ...
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Leukocyte adhesion deficiency/congenital disorder of glycosylation IIc (LAD II/CDG IIc) is a genetic disease characterized by a decreased expression of fucose in glycoconjugates, resulting in leukocyte adhesion deficiency and severe morphological and neurological abnormalities. The biochemical defect is a reduced transport of guanosine diphosphate-L-fucose (GDP-L-fucose) from cytosol into the Golgi compartment, which reduces its availability as substrate for fucosyltransferases. The aim of this study was to determine the effects of a limited supply of GDP-L-fucose inside the Golgi on core fucosylation (a1,6-fucose linked to core N-acetylglucosamine [GlcNAc]) of N-linked glycans in LAD II fibroblasts. The results showed that, although [3H]fucose incorporation was generally reduced in LAD II cells, core fucosylation was affected to a greater extent compared with other types of fucosylation of N-linked oligosaccharides. In particular, core fucosylation was found to be nearly absent in biantennary ...
Looking for online definition of 2-amino-2-deoxy-d-galactose in the Medical Dictionary? 2-amino-2-deoxy-d-galactose explanation free. What is 2-amino-2-deoxy-d-galactose? Meaning of 2-amino-2-deoxy-d-galactose medical term. What does 2-amino-2-deoxy-d-galactose mean?
Glycosylation drives critical processes important for mammalian cell-cell and cell-matrix interactions. Alpha-l-fucose (α-l-f) is a key monosaccharide component of oligosaccharides that has been found to be overexpressed during tumor progression. Modification of cell surface fucosylation, we hypothesized, alters tumor cell phenotype and function at the end of the neoplastic progression cascade including tumor invasion. Alpha-l-fucosidase (α-l-fase) is a glycosidase that specifically removes (α-l-f) from oligosaccharide sites. We first verified the effectiveness of the α-l-fase to specifically decrease the level of α-l-f on the cell surface of several human breast cancer cell lines and also examined the recovery time for these cells to repopulate their surfaces. To investigate the potential effect of defucosylation on tumor functions, we studied the proliferation, and invasion in vitro of human breast cancer MDA-MB-231 cells as the representative cell model. We further examined several ...
Mucins are high-molecular weight glycoproteins (0.25-20 MDa) containing one or more domains that are heavily O-glycosylated. Their implications as targets for cancer treatment have increased the interest in these glycoproteins, mainly in the fields of vaccines and antibodies. However, mucins present high heterogeneity, posing challenges that affect purification processes and quality control analysis. In that sense, it is necessary to develop and improve downstream processes and analytical methods to characterize these products. Here a tool based on biolayer interferometry analysis to improve mucins detection and quantification in a fast, simple and label free-way is presented. Taking advantage of lectin recognition of mucins carbohydrate structures, several lectins were evaluated and immobilized on streptavidin biosensors. Different assay conditions were optimized and the most suitable lectin, Aleuria aurantia lectin (AAL), was selected. Bovine Submaxillary Gland and human MUC5B mucins were ...
Définitions de 1 3 alpha l fucosidase, synonymes, antonymes, dérivés de 1 3 alpha l fucosidase, dictionnaire analogique de 1 3 alpha l fucosidase (anglais)
Structural and functional evidence indicate that the fucose-binding lectin present on bull sperm is a seminal plasma protein secreted by the seminal vesicles called PDC-109 (BSP-A1/A2) (Gwathmey et al 2003, Ignotz et al 2001). Upon ejaculation, this protein associates with the sperms plasmatic membrane enabling it to bind to the oviductal epithelium (figure 1). This proposed mechanism explains the limited binding capacity of sperm that have not been in contact with seminal fluids i.e., epididimal sperm (Gwathmey et al 2003, Petrunkina et al 2001). Furthermore, excess PDC-109 in seminal plasma may block sperm binding sites on posterior portions of the female reproductive tract facilitating sperm transport to the oviducts (Suárez, 2002). Mucus secretions. Overstreet and Cooper (1975) first observed that rabbit sperm collected from the isthmus of does before ovulation were immotile or weakly motile. This observation is interpreted as a transitory inhibition or suppression of sperm flagellar ...
Electrospray mass spectrometry (ESI-MS) was used to measure the masses of an intact dimeric monoclonal antibody (Mab) and assess the fucosylation level. The Mab under study was EG2-hFc, a chimeric human-camelid antibody of about 80 kDa (A. Bell et al., Cancer Lett., 2010, 289(1), 81-90). It was obtained from Glycomics & Glycoproteomics: From Analytics to Function
There is a clinical need for methods that can quantify regional hepatic function non-invasively in patients with cirrhosis. Here we validate the use of 2-[(18)F]fluoro-2-deoxy-d-galactose (FDGal) PET/CT for measuring regional metabolic function to this purpose, and apply the method to test the hypothesis of increased intrahepatic metabolic heterogeneity in cirrhosis ...
This is a single-center, randomized, two-arm, study evaluating the safety, feasibility and immunogenicity of neoadjuvant degarelix(Arm A) or BMS-986218 plus degarelix (Arm B) prior to radical prostatectomy in men with high-risk localized prostate cancer (neo-RED-P). Our primary objective is to characterize safety, tolerability, and feasibility of degarelix with or without BMS-986218 in the neoadjuvant setting. The trial will monitor toxicity and safety, as well as surgery related adverse events. The secondary objectives will be to evaluate an immune response consistent with the proposed mechanism of action of BMS-986218, depletion of Tregs, and to assess the pathologic complete response rate, PSA response rate and time-to-PSA recurrence following treatment ...
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/GCP/CCG/CCG-0004-10.pdf]Fucose BSA Colloidal Gold Conjugate, 1mL
In gene-targeted mouse models for cystic fibrosis (CF), the disease is mainly manifested by mucus obstruction in the intestine. To explore the mucus composition, mucins insoluble and soluble in 6 M guanidinium chloride were purified by three rounds of isopycnic ultracentrifugation from the small and large intestines of CF mice (Cftr(m1UNC)/Cftr(m1UNC)) and compared with wild-type mice. The amino acid composition was typical of that for mucins and showed increased amounts of the insoluble (2.5-fold increase) and soluble (7-fold increase) mucins in the small intestine of the CF mice compared with wild-type mice. Mucins from the large intestine of both wild-type and CF mice showed a high but constant level of fucosylation. In contrast, the insoluble and soluble mucins of the small intestine in CF mice revealed a large increase in fucose, whereas those of wild-type mice contained only small amounts of fucose. This increased fucosylation was analysed by releasing the O-linked oligosaccharides ...
Fucose galactose carbohydrates have been shown to induce neuronal outgrowth. The invention includes methods of inducing neuronal outgrowth using carbohydrates, assemblies, and polymers bearing fucose-
1OD3: Structure and Ligand Binding of Carbohydrate-Binding Module Cscbm6-3 Reveals Similarities with Fucose-Specific Lectins and Galactose-Binding Domains
Shop L-fucose mutarotase ELISA Kit, Recombinant Protein and L-fucose mutarotase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Host fucosylation increases tolerance of a pathogena, Difference in % weight loss between LPS-injected C. rodentium-infected and uninfected mice (mean±s.e.m.;
PROCESS FOR PRODUCING AN OXIDIZED NANOFIBRILLAR CELLULOSE HYDROGEL | METHOD AND APPARATUS FOR CONTROLLING THE CATALYTIC OXIDATION OF CELLULOSE | MULTISPECIFIC ANTIBODIES | OPTIMIZED CROSS-SPECIES SPECIFIC BISPECIFIC SINGLE CHAIN ANTIBODY CONTRUCTS | C-MET ANTIBODY COMBINATIONS |
The Golm Metabolome Database (GMD) facilitates the search for and dissemination of mass spectra from biologically active metabolites quantified using GC-MS.
The Golm Metabolome Database (GMD) facilitates the search for and dissemination of mass spectra from biologically active metabolites quantified using GC-MS.
Fc gamma RIIIA/CD16a Lysates available through Novus Biologicals. Browse our Fc gamma RIIIA/CD16a Lysate catalog backed by our Guarantee+.
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J:185112 Park D, Choi D, Lee J, Lim DS, Park C, Male-like sexual behavior of female mouse lacking fucose mutarotase. BMC Genet. 2010;11:62 ...
TY - JOUR. T1 - O-fucosylation of muscle agrin determines its ability to cluster acetylcholine receptors. AU - Kim, Mi Lyang. AU - Chandrasekharan, Kumaran. AU - Glass, Matthew. AU - Shi, Shaolin. AU - Stahl, Mark C.. AU - Kaspar, Brian. AU - Stanley, Pamela. AU - Martin, Paul T.. PY - 2008/10/29. Y1 - 2008/10/29. N2 - Protein O-fucosyltransferase 1 (Pofut1) transfers fucose to serine or threonine on proteins, including Notch receptors, that contain EGF repeats with a particular consensus sequence. Here we demonstrate that agrin is O-fucosylated in a Pofut1-dependent manner, and that this glycosylation can regulate agrin function. Fucosylation of recombinant C45 agrin, both active (neural, z8) and inactive (muscle, z0) splice forms, was eliminated when agrin was overexpressed in Pofut1-deficient cells or by mutation of a consensus site for Pofut1 fucosylation (serine 1726 in the EGF4 domain). Loss of O-fucosylation caused a gain of function for muscle agrin such that it stimulated AChR ...
Lectins, a group of proteins that bind to cell surface carbohydrates and play important roles in innate immunity, are widely used experimentally to distinguish cell types and to induce cell proliferation. Eel serum lectins have been useful as anti-H hemagglutinins and also in lectin histochemistry as fucose-binding lectins (fucolectins), but their structures have not been determined. Here we report the primary structures and the sites of synthesis of eel fucolectins. Eel serum fucolectins were separated by two-dimensional gel electrophoresis and sequenced. cDNA cloning, based on the amino acid sequence information, and Northern blot analysis indicated that 1) the fucose-binding lectins are secretory proteins and have unique structures among the lectins, exhibiting only weak similarities to frog pentraxin, horseshoe crab tachylectin-4, and fly fw protein; 2) there are at least seven closely related members; and 3) their messages are abundantly expressed in the liver and in significant levels in ...
Introduction: The aim of this study was to compare fucose and sialic acid residue expression on fibronectin and alpha(1)-acid glycoprotein in the. seminal plasma PF-6463922 solubility dmso of men suspected of infertility and suffering from leukocytospermia.. Subjects and methods: Seminal ejaculates were collected from 27 leukocytospermic and 18 healthy, normozoospermic men. The relative degree of fucosylation and sialylation of fibronectin and alpha(1)-acid glycoprotein was estimated by ELISA using fucose and sialic acid specific lectins from Aleuria aurantia, Lotus tetragonolobus, and Ulex europaeus as well as Maackia amurensis and Sambucus nigra, respectively.. Results: Leukocytospermic seminal fibronectin, in comparison with fibronectin of normal fertile group, showed lower relative reactivity with AAL, LTA and UEA, and higher reactivity with MAA and SNA, while the AGP of the leukocytospermic group was less reactive with AAL, and the relative reactivity. buy 5-Fluoracil with LTA and MAA was ...
This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the MFNG (GeneID: 4242) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. [provided by RefSeq, May 2018 ...
Notch is a transmembrane receptor for the transmembrane ligands Delta and Jagged (also known as Serrate). Signaling by Notch is important for establishing boundaries during development (see accompanying article by Fortini). Fringe modifies Notch signaling when expressed in Notch-expressing cells. Two groups, Brückner et al. and Moloney et al., show that Fringe is a glycosyltransferase that catalyzes the elongation of O-linked fucose residues on the epidermal growth factor repeats of the Notch receptor. Brückner et al. show that coexpression of Fringe and Notch enhances the interaction between Notch and Delta and that the glycosyltransferase activity of Fringe is essential for this activity. Moloney et al. show that when Notch is modified by Fringe, activation of Notch by Jagged1 is inhibited. Thus, carbohydrate modification of the Notch receptor fine-tunes the cellular responsiveness to Notch ligands, allowing the establishment of discrete boundaries of Notch signaling. Carbohydrate ...
Breast-fed infants have Bifidobacterium-rich gut microbiota compared to infants fed formula. Fucosylated oligosaccharides are the major components of human
Direct mass spectrometric analysis of aberrant protein glycosylation is a challenge to the current analytical techniques. Except lectin affinity chromatography, no other glycosylation enrichment techniques are available for analysis of aberrant glycosylation. In this study, we developed a combined chemical and enzymatic strategy as an alternative for the mass spectrometric analysis of aberrant glycosylation. Sialylated glycopeptides were enriched with reverse glycoblotting, cleaved by endoglycosidase F3 and analyzed by mass spectrometry with both neutral loss triggered MS3 in collision induced dissociation (CID) and electron transfer dissociation (ETD). Interestingly, a great part of resulted glycopeptides were found with fucose attached to the N-acetylglucosamine (N-GlcNAc), which indicated that the aberrant glycosylaton that is carrying both terminal sialylation and core fucosylation was identified. Totally, 69 aberrant N-glycosylation sites were identified in sera samples from hepatocellular ...
Exhibits fucose binding activity and racemase and epimerase activity, acting on carbohydrates and derivatives. Involved in several processes, including female mating behavior; fucose metabolic process; and negative regulation of neuron differentiation. Orthologous to human FUOM (fucose mutarotase ...
The goal of this clinical research study is to learn if it is safe and feasible to transplant changed cord blood for patients with leukemia or lymphoma. Researchers also want to learn if this can help to control the disease.. The cord blood will be changed to make use of sugar that is found in small amounts in blood cells. It plays a role in signaling where in the body the transplanted cells should go to. Adding more sugars to the cord blood cells in the laboratory is designed to help the cord blood cells find their way faster to the bone marrow. This may help your blood counts to recover faster. This process is called fucosylation.. Anti-thymocyte globulin (ATG) is a protein that removes immune cells that cause damage to the body.. Clofarabine is designed to interfere with the growth and development of cancer cells.. Fludarabine is designed to interfere with the DNA (genetic material) of cancer cells, which may cause the cancer cells to die. This chemotherapy is also designed to block your ...
Mannose can randomize to glucose, galactose, and fucose. However, because the label in [3H]2-mannose is on the hydrogen of C-atom 2, the label would be predominantly found in mannose and fucose. In rare instances, the label from [3H]2-mannose can be found in glucose and lactic acid, suggesting that during oxidation and reduction reaction resulting in the epimerization of mannose to glucose, the label may be transferred from C-atom 2 to C-atom 1 (see Ref. 31). Thus, the major labeled spot in Fig. 1⇑ could be due to a glycan modifying a lipid and/or a protein. For three reasons we believe that the [3H]2-mannose-labeled compound is a glycolipid and not a glycopeptide or glycoprotein. First, the control Db-sol, also a glycoprotein with two N-linked glycans, processed in the same manner as sCD1d1, does not contain the tritiated spots seen with the CD1 sample (Fig. 1⇑). Second, the CD1d1-associated [3H]2-mannose-labeled ligands are PI-PLC sensitive as described below. Third, sialic acid-containing ...
Modulation of protein function by covalent modification is of increasing importance as a factor in disease and as a therapeutic target. The discovery of new protein modifications and their function remains a challenge. Using protein/peptide purification and mass spectrometry as well as molecular and cell biology techniques, we focus on two unique forms of glycosylation in secreted proteins, C-mannosylation of tryptophan and O-fucosylation of serine or threonine residues. About 50 examples of the former modification are known, in organisms ranging from C. elegans to man. We are studying enzymes involved in the process and analysing specific proteins lacking the modification. Of the two kinds of O-fucose-linked polysaccharides known, O-linked tetrasaccharide appears to function in intercellular signalling. We have found the first protein containing the product of the second pathway, i.e. Glc-Fuc-O-Ser/Thr and are seeking its function ...
Substantial evidence suggests that cell surface carbohydrate antigens, particularly those containing fucose residues, are related to cancer malignancy. To investigate the mechanisms underlying cell...
T-cells infiltrating sites of inflammation of the skin typically express the cutaneous lymphocyte-associate antigen (CLA). This antigen is defined by the binding of the monoclonalCLA antibody HECA-452. The CLA antigen is a fucose-containing oligosaccharide and is found on many of the ligands that are recognized by the adhesion proteins P-selectin and E-selectin.
Yuen R, Schachter H (1972). "L-Fucose metabolism in mammals. I. Port liver L-fuconate hydro-lyase". Can. J. Biochem. 50 (7): ...
1992, ; New Latin noun fucosum, fucose; New Latin masculine gender adjective fucosus (sic), containing fucose in the cell wall ...
Heath EC, Ghalambor MA (1962). "The metabolism of L-fucose. I. The purification and properties of L-fuculose kinase". J. Biol. ...
Addition of a fucose alone creates the H-antigen, present in people with blood type O. By adding a galactose onto this ... Addition of fucose sugars to serine and threonine residues is an unusual form of O-glycosylation that occurs in the endoplasmic ... Once the core O-fucose has been added, it is often elongated by addition of GlcNAc, galactose and sialic acid. Notch is an ... Addition of fucose sugars by fucosyltransferases forms Lewis epitopes and the scaffold for blood group determinants. ...
Dahms AS, Anderson RL (1972). "D-Fucose metabolism in a pseudomonad. 3. Conversion of D-fuconate to 2-keto-3-deoxy-D-fuconate ...
Fucose supplementation has had a partial effect on some SLC35C1-CDG patients. The first CDG patients (twin sisters) were ... caused by a GDP-fucose (Fuc) transporter deficiency. All N-linked oligosaccharides originate from a common lipid-linked ...
Other names in common use include D-arabinose(L-fucose) isomerase, D-arabinose isomerase, L-fucose isomerase, and D-arabinose ... Green M, Cohen SS (1956). "Enzymatic conversion of L-fucose to L-fuculose". J. Biol. Chem. 219 (2): 557-568. PMID 13319278. ...
GDP fucose-lactose fucosyltransferase, guanosine diphospho-L-fucose-lactose fucosyltransferase, guanosine diphosphofucose-beta- ... The systematic name of this enzyme class is GDP-beta-L-fucose:beta-D-galactosyl-R 2-alpha-L-fucosyltransferase. Other names in ... CS1 maint: discouraged parameter (link) Grollman AP (1966). "GDP-L-fucose:lactose fucosyltransferase from mammary gland". ... the two substrates of this enzyme are GDP-beta-L-fucose and beta-D-galactosyl-R, whereas its two products are GDP and alpha-L- ...
In Arabidopsis, terminal sugars such as fucose are proposed to be added by AtFUT4 (a fucosyl transferase) and AtFUT6 in the ... Two α-fucosyltransferase genes, FUT4 and FUT6, both belonging to GT37 family, encode enzymes which add α-1,2-fucose residues to ... L-fucose (Fuc), D-glucopyranose (Glcp), D-glucuronic acid (GlcA) and its 4-O-methyl derivative, and D-galacturonic acid (GalA) ... fucose (Fuc), and glucuronic acid (GlcA). These AG glycan moieties are assembled by glycosyltransferases (GTs). O-glycosylation ...
Fucoidan is a polymer composed of sulfated L-fucose. Carrageenans are isolated from algae. Hyaluronan functions as a heparinoid ...
Chemically, it is a methyl ether of D-fucose. Sarmentose, a related deoxy sugar Digitalose, Merck Index, 12th Edition, 3202 ...
Alpha-Fucosidase is an enzyme that breaks down fucose. Fucosidosis is an autosomal recessive lysosomal storage disease caused ... by defective alpha-L-fucosidase with accumulation of fucose in the tissues. Different phenotypes include clinical features such ...
O-fucose modifications have only been detected in hEGF-like domains and they are important for the proper folding of the hEGF- ... Factor VII exhibits an O-linked fucose at Ser60. Multiple cbEGF domains are often connected by one or two amino acids to form ... These post-translational modifications include O-glycosylations, mostly O-fucose modifications, and β-hydroxylation of ...
... similarly fucose presents the same for certain bacteria and helminths; and glucans are present on mycobacteria and fungi. In ...
Park D, Choi D, Lee J, Lim DS, Park C (July 2010). "Male-like sexual behavior of female mouse lacking fucose mutarotase". BMC ... Those mice who retained the gene fucose mutarotase (FucM) were attracted to male mice. In interviews to the press, researchers ...
Dang S, Sun L, Huang Y, Lu F, Liu Y, Gong H, Wang J, Yan N (October 2010). "Structure of a fucose transporter in an outward- ...
... fucose-specific beta-1,3-N-acetylglucosaminyltransferase (EC 2.4.1); globotriosylceramide beta-1,3-GalNAc transferase (EC 2.4. ...
Park D, Choi D, Lee J, Lim DS, Park C (July 2010). "Male-like sexual behavior of female mouse lacking fucose mutarotase". BMC ... Researchers found that disabling the fucose mutarotase (FucM) gene in laboratory mice - which influences the levels of estrogen ...
Other names in common use include L-fucose dehydrogenase, (2S,3R)-aldose dehydrogenase, dehydrogenase, L-fucose, and L-fucose ( ... Schachter H, Sarney J, McGuire EJ, Roseman S (1969). "Isolation of diphosphopyridine nucleotide-dependent L-fucose ...
... a generalized defect in fucose metabolism". The Journal of Pediatrics. 134 (6): 681-8. doi:10.1016/S0022-3476(99)70281-7. PMC ...
... converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved ... GDP-L-fucose synthetase is an enzyme that in humans is encoded by the TSTA3 gene. Tissue specific transplantation antigen P35B ... PDBe-KB provides an overview of all the structure information available in the PDB for Human GDP-L-fucose synthase v t e. ... 1996). "Synthesis of GDP-L-fucose by the human FX protein". J. Biol. Chem. 271 (44): 27274-9. doi:10.1074/jbc.271.44.27274. ...
Thomas CJ, Surolia A (2000). "Mode of molecular recognition of L-fucose by fucose-binding legume lectins". Biochemical and ...
Fucose-1-phosphate guanylyltransferase is an enzyme that in humans is encoded by the FPGT gene. L-fucose is a key sugar in ... This pathway involves the phosphorylation of L-fucose to form beta-L-fucose-1-phosphate, and then condensation of the beta-L- ... fucose-1-phosphate with GTP by fucose-1-phosphate guanylyltransferase to form GDP-beta-L-fucose. GRCh38: Ensembl release 89: ... There are two alternate pathways for the biosynthesis of GDP-fucose; the major pathway converts GDP-alpha-D-mannose to GDP-beta ...
... is the process of adding fucose sugar units to a molecule. It is a type of glycosylation.[citation needed] It is ...
The addition of O-fucose by POFUT1 is absolutely necessary for notch function, and, without the enzyme to add O-fucose, all ... If Fringe adds a GlcNAc to the O-fucose sugar then the subsequent addition of a galactose and sialic acid will occur. In the ... An O-glucose sugar may be added between the first and second conserved cysteines, and an O-fucose may be added between the ... The Notch1-Dll4 structure also illuminated a direct role for Notch O-linked fucose and glucose moieties in ligand recognition, ...
... s encode O-fucose specific β-1,3-N-Acetylglucosaminyltransferases (GlcNAcT) glycosyltransferases. The gene products ...
Requirement of core alpha1,6-linked fucose and a polypeptide chain for polysialylation". The Journal of Biological Chemistry. ...
The systematic name of this enzyme class is dTDP-D-fucose:NADP+ oxidoreductase. This enzyme is also called dTDP-4-keto-6- ... the two substrates of this enzyme are dTDP-D-fucose and NADP+, whereas its 3 products are dTDP-4-dehydro-6-deoxy-D-glucose, ... "A novel NDP-6-deoxyhexosyl-4-ulose reductase in the pathway for the synthesis of thymidine diphosphate-D-fucose". J. Biol. Chem ... is an enzyme that catalyzes the chemical reaction dTDP-D-fucose + NADP+ ⇌ {\displaystyle \rightleftharpoons } dTDP-4-dehydro-6- ...
Other names in common use include NAD+-pentose-dehydrogenase, and arabinose(fucose)dehydrogenase. Palleroni NJ; Doudoroff M ( ...
More than 10 kinds of connected Saccharides like Fucose are defined as Fucoidan. It is expected that the original Fucoidan ... Variety of saccharides such as Fucose, Xylose, Mannose, Glucose, Galactose, Glucuronic acid, and Galacturonic acid are ...
Fucose is released from fucose-containing polymers by an enzyme called α-fucosidase found in lysosomes. l-Fucose has several ... Fucose is the fundamental sub-unit of the seaweed polysaccharide fucoidan. The α(1→3) linked core of fucose is a suspected ... In the fucose-containing glycan structures, fucosylated glycans, fucose can exist as a terminal modification or serve as an ... In human N-linked glycans, fucose is most commonly linked α-1,6 to the reducing terminal β-N-acetylglucosamine. However, fucose ...
In enzymology, a L-fucose isomerase (EC 5.3.1.25) is an enzyme that catalyzes the chemical reaction L-fucose ⇌ {\displaystyle \ ... The systematic name of this enzyme class is L-fucose aldose-ketose-isomerase. This enzyme participates in fructose and mannose ... Lu Z, Lin EC (1989). "The nucleotide sequence of Escherichia coli genes for L-fucose dissimilation". Nucleic Acids Res. 17 (12 ... Seemann JE, Schulz GE (October 1997). "Structure and mechanism of L-fucose isomerase from Escherichia coli". J. Mol. Biol. 273 ...
L-(-)-Fucose, tetrakis(trimethylsilyl) ether, methyloxime (syn). *L-(-)-Fucose, tetrakis(trimethylsilyl) ether, methyloxime ( ... Fucose, MEOX-4TMS. *Formula: C19H47NO5Si4 ...
Fucose is a deoxyhexose that is present in a wide variety of organisms. In mammals, fucose-containing glycans have important ... Fucose deficiency is accompanied by a complex set of phenotypes both in humans with leukocyte adhesion deficiency type II (LAD ... Two pathways for the synthesis of GDP-fucose operate in mammalian cells, the GDP-mannose-dependent de novo pathway and the free ... Fucose: biosynthesis and biological function in mammals.. Becker DJ1, Lowe JB. ...
... *Download PDF Copy ... Watanabe, S. (2020) Characterization of L-2-keto-3-deoxyfuconate aldolases in a nonphosphorylating L-fucose metabolism pathway ... strictly anaerobic bacterium Veillonella ratti possesses a gene cluster that appears to be related to L-fucose metabolism and ... and report the discovery of another L-2-keto-3-deoxyfuconate aldolase involved in the same non-phosphorylating L-fucose pathway ...
Fucose in N-glycans: from plant to man.. Staudacher E1, Altmann F, Wilson IB, März L. ...
Get Fucose at Spectrum Chemical. SpectrumChemical.com carries a full line of fine chemicals, lab appliances and lab supplies. ... L-(-)-Fucose TCI-F0065 , 2438-80-4 , C6H12O5 , TCI America L-(-)-Fucose ...
GDP-fucose Protein O-Fucosyltransferase 1 Enzyme, Fucose, Serine, Threonine, Epidermal growth factor, Notch signaling pathway ... GDP-fucose Protein O-Fucosyltransferase 2 Enzyme, Fucose, Serine, Threonine, Thrombospondin, Glycosyltransferase, Golgi ... use of fucose sugar and its derivative as tumor marker Biochemistry, biophysics Noor Publishing (2018-03-30) - ISBN-13: 978- ... L-Fucose Isomerase Protein, Flavonoid biosynthesis, Uridine diphosphate, Molecule. Organic chemistry Polic (2012-05-05) - ISBN- ...
recognizes an abnormal pattern of L-fucose on postischemic renal tubule cells and activates a destructive inflammatory response ... Collectin-11 detects stress-induced L-fucose pattern to trigger renal epithelial injury. ... Collectin-11 detects stress-induced L-fucose pattern to trigger renal epithelial injury. ... binding that was induced by cell stress under hypoxic or hypothermic conditions and prevented by specific removal of L-fucose. ...
Carbohydrate-dependent B cell activation by fucose-binding bacterial lectins. By Isabel Wilhelm, Ella Levit-Zerdoun, Johanna ... Fucose-specific bacterial lectins bind to and stimulate mouse B cells, inducing an immune response in vivo. ... Carbohydrate-dependent B cell activation by fucose-binding bacterial lectins. By Isabel Wilhelm, Ella Levit-Zerdoun, Johanna ... Fucose-specific bacterial lectins bind to and stimulate mouse B cells, inducing an immune response in vivo. ...
Wilhelm et al. showed that fucose-binding bacterial lectins bound to and activated mouse B cells in vitro, which depended on ... Fucose-specific bacterial lectins bind to and stimulate mouse B cells, inducing an immune response in vivo. ... Fucose-specific bacterial lectins bind to and stimulate mouse B cells, inducing an immune response in vivo. ... Carbohydrate-dependent B cell activation by fucose-binding bacterial lectins Message Subject. (Your Name) has forwarded a page ...
Antibodies for proteins involved in fucose biosynthetic process pathways, according to their Panther/Gene Ontology ...
Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series ... Here, we show that the mice immunized with a l-fucose (Fuc)-enriched Reishi polysaccharide fraction (designated as FMS) induce ... Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series ...
The enzymatic approach using l-fucose isomerase, which interconverts l-fucose and l-fuculose, can be an efficient way of ... The enzymatic approach using l-fucose isomerase, which interconverts l-fucose and l-fuculose, can be an efficient way of ... Enzymatic synthesis of l-fucose from l-fuculose using a fucose isomerase fromRaoultellasp. and the biochemical and structural ... L-fucose isomerase. A, B, C, D, E, F. 612. Raoultella planticola. Mutation(s): 0 Gene Names: fucI, NCTC13095_01108, DN603_10810 ...
Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a ... GDP-L-fucose synthase (fcl). This subpathway is part of the pathway GDP-L-fucose biosynthesis via de novo pathway, which is ... GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH.UniRule annotation. ,p>Manual validated information which ... GDP-L-fucose synthaseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic ...
Furthermore, we demonstrate that the Std fimbriae-fucose interaction is necessary for bacterial colonization of the intestine ...
GDP-L-fucose biosynthesis pathways, according to their Panther/Gene Ontology Classification ...
Although fucose catabolic pathways have been enzymatically evaluated, the effects of fucose as a carbon source on intracellular ... To elucidate the effects of fucose on cellular metabolism and to find clues for efficient conversion of fucose into bio-based ... When fucose was the carbon source for E. coli, integration of the two omics analyses revealed that excess gluconeogenesis and ... This is the first multi-omics study investigating the effects of fucose on cellular metabolism in E. coli. These omics data and ...
... offers a comprehensive analysis on L-Fucose industry, standing ... 9.1 Cost Structure Analysis of L-Fucose. 9.2 Raw Materials Cost Analysis of L-Fucose. 9.3 Labor Cost Analysis of L-Fucose. 9.4 ... CHAPTER 1 OVERVIEW OF L-FUCOSE. 1.1 Definition of L-Fucose in This Report. 1.2 Commercial Types of L-Fucose. 1.2.1 ... 1.4 Development History of L-Fucose. 1.5 Market Status and Trend of L-Fucose 2013-2023. 1.5.1 United States L-Fucose Market ...
L-Fucose. Description. Fucose is a hexose deoxy sugar with the chemical formula C6H12O5. L-fucose (6-deoxy-L-galactose) is a ... All fucosyltransferases utilize a nucleotide-activated form of fucose, GDP-fucose, as a fucose donor in the construction of ... Fucose is secreted in urine when the liver is damaged. Free L-fucose in serum and urine can be used as a marker for cancer, ... In fucose-containing glycan structures, fucosylated glycans, fucose can exist as a terminal modification or serve as an ...
The Streptococcus pneumoniae fucose utilization operon includes a gene encoding a virulence factor that belongs to family 98 in ... The Streptococcus pneumoniae fucose utilization operon includes a gene encoding a virulence factor that belongs to family 98 in ... Structure of a Streptococcus pneumoniae fucose binding module in complex with the blood group H-trisaccharide. *DOI: 10.2210/ ... This protein contains a C-terminal triplet of fucose binding modules that have significant amino acid sequence identity with ...
Subject: Re: AMBER: [amber/glycam] dealing with fucose.... From: Lachele Foley (Lists) (lf.list_at_gmail.com). Date: Thu Jul 10 ... Reply: Waqas Nasir: "Re: AMBER: [amber/glycam] dealing with fucose..." *Messages sorted by: [ date ] [ thread ] [ subject ] [ ... Subject: Re: AMBER: [amber/glycam] dealing with fucose... , , Hi Waqas, , , Before we get off onto a lengthy discussion... You ... Reply: Waqas Nasir: "Re: AMBER: [amber/glycam] dealing with fucose..." *Messages sorted by: [ date ] [ thread ] [ subject ] [ ...
The cytotoxicity of an L-fucose-bound liposome carrying sorafenib (Fuc-Lip-sorafenib) with HDACi was assessed in vivo and in ... The delivery of Cy5.5-encapsulated L-fucose-liposomes (Fuc-Lip-Cy5.5) to a Lens Culinaris agglutinin-reactive fraction of α- ... the regulation of FUT8 expression by p53 and develop a novel therapeutic strategy for targeting HCC cells using L-fucose- ...
Distribution of Fucose-Containing Xyloglucans in Cell Walls of the mur1 Mutant of Arabidopsis Glenn Freshour, Christopher P. ... 1996) Substitution of l-fucose by l-galactose in cell walls of Arabidopsis mur1. Science 272:1808-1810. ... 1993) Altered growth and cell walls in a fucose-deficient mutant of Arabidopsis. Science 261:1032-1035. ... Distribution of Fucose-Containing Xyloglucans in Cell Walls of the mur1 Mutant of Arabidopsis ...
FUCOSE-CONTAINING BACTERIAL BIOPOLYMER. The presently disclosed subject matter concerns a microbial biopolymer comprising ... fucose in its composition. This biopolymer consists of a polysaccharide... 2017/0349671. PROCESS FOR PRODUCING AN OXIDIZED ...
D-fucose ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs. ...
This lectin has proven to have a strong affinity toward L-fucose according to the results of hemagglutination inhibition assay. ... The molecular weight of L-fucose specific lectin subunit, a dimeric substance, showed 35,000 (Fig. 1). This lectin shows 26% ... 2)Molecular cloning and overexpression of fleA gene encoding a fucose-specific lectin of Aspergillus oryzae H. Ishida, T. ... Especially, the ones with fucose typically possess physiological properties. Therefore, these lectins are often used for such ...
We determined the specificity of the lectin towards l-fucose and fucosylated oligosaccharides, including alpha 1-6 linked core- ... fucose, which is an important marker for cancerogenesis. Other biologically relevant saccharides such as sialic acid, d-mannose ... A Soluble Fucose-Specific Lectin from Aspergillus fumigatus Conidia - Structure, Specificity and Possible Role in Fungal ... We determined the specificity of the lectin towards l-fucose and fucosylated oligosaccharides, including alpha 1-6 linked core- ...
Metabolism of L-fucose and L-rhamnose in Escherichia coli: aerobic-anaerobic regulation of L-lactaldehyde dissimilation.. L ... Metabolism of L-fucose and L-rhamnose in Escherichia coli: aerobic-anaerobic regulation of L-lactaldehyde dissimilation. ... Metabolism of L-fucose and L-rhamnose in Escherichia coli: aerobic-anaerobic regulation of L-lactaldehyde dissimilation. ... Metabolism of L-fucose and L-rhamnose in Escherichia coli: aerobic-anaerobic regulation of L-lactaldehyde dissimilation. ...
The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of ... Our previous studies indicated that 70% of Fut8-deficient (Fut8(-/-)) mice that lack core fucose structure die within 3 days ... which may compensate for the loss of core fucose functions. ...
  • The Gram-negative, strictly anaerobic bacterium Veillonella ratti possesses a gene cluster that appears to be related to L-fucose metabolism and contains a putative dihydrodipicolinate synthase DHDPS/NAL protein (FucH). (news-medical.net)
  • Intriguingly, some of the GDP- l -fucose synthase-reactive cells could also be stimulated by a bacterial version of the enzyme. (sciencemag.org)
  • GDP-L-Fucose Synthase antibody LS-C702921 is a Cy3-conjugated rabbit polyclonal antibody to mouse GDP-L-Fucose Synthase (TSTA3). (lsbio.com)
  • GDP-L-Fucose Synthase antibody LS-C158282 is an unconjugated rabbit polyclonal antibody to mouse GDP-L-Fucose Synthase (TSTA3). (lsbio.com)
  • P. falciparum presents homologues of GDP-mannose 4,6-dehydratase and GDP-L-fucose synthase enzymes that are active in vitro, indicating that most GDP-fucose is formed by a de novo pathway that involves the bioconversion of GDP-mannose. (conicet.gov.ar)
  • Fluorescence microscopy of epitope-tagged versions of P. falciparum GDP-mannose 4,6-dehydratase and GDP-L-fucose synthase expressed in transgenic 3D7 parasites shows that these enzymes localize in the cytoplasm of P. falciparum during the intraerythrocytic developmental cycle. (conicet.gov.ar)
  • However, fucose at the non-reducing termini linked α-1,2 to galactose forms the H antigen, the substructure of the A and B blood group antigens. (wikipedia.org)
  • L-fucose (6-deoxy-L-galactose) is a monosaccharide that is a common component of many N- and O-linked glycans and glycolipids produced by mammalian cells. (hmdb.ca)
  • The disaccharide motif fucose-α(1-2)-galactose (Fucα(1-2)Gal) is involved in many important physiological processes, such as learning and memory, inflammation, asthma, and tumorigenesis. (caltech.edu)
  • The content of galactose (12, 13) , bisecting N -acetylglucosamine (14, 15) , and fucose (16, 17) in the antibody oligosaccharide have each been reported to effect ADCC. (aacrjournals.org)
  • In medium containing extensively dialysed serum the secretion of leucine- and fucose-labelled myeloma protein can be restored by the addition of 250μ m-d -mannose, 250μ m-d -galactose and 250μ m -glucosamine. (biochemj.org)
  • The reaction product was analyzed and the produced galactose-fucose disaccharides were purified. (deepdyve.com)
  • The objectives of the study are to collect natural history and treatment-related data of patients diagnosed with Phosphoglucomutase 1-CDG, MPI-CDG or SLC35C1-CDG who are either treated with or without D-galactose, D-mannose and L-fucose , respectively, as well as patients with other CDGs who are treated with one of the sugars. (thefreedictionary.com)
  • Fucoidans are complex sulfated polysaccharides consisting mainly of sulfated L-fucose and variable proportions of galactose, mannose, xylose, glucose, rhamnose and uronic acids (Usov & Bilan, 2009). (thefreedictionary.com)
  • it indicated that they were composed of L-fucose , D-mannose, D-glucose, and D-galactose in molar ratios of 1.4: 1: 1.3: 1.7. (thefreedictionary.com)
  • 6-deoxy-L-beta-galactose;L-FUCOSE, 99.6% MIN. (bosschemical.com)
  • Using a liquid chromatography-tandem mass spectrometry- based method, we identified and quantified the pools of UDP-glucose, UDP-galactose, UDP-N-acetylglucosamine, GDP-mannose, and GDP-fucose in Plasmodium falciparum intraerythrocytic life stages. (conicet.gov.ar)
  • The columns detail the proportions of fucose, galactose, sulphate, and polyphenol that are present in the fucoidan component of the sample. (hindawi.com)
  • CTLs whose CRD contain EPN (Glu-Pro-Asn) tripeptide motifs have potential to bind mannose (Man), N-acetylglucosamine (GlcNAc), glucose (Glc) and l-fucose (Fuc), whereas those with QPD (Glu-Pro-Asp) tripeptide motifs bind galactose (Gal) and N-acetylgalactosamine (GalNAc). (scripps.edu)
  • All fucosyltransferases utilize a nucleotide-activated form of fucose, GDP-fucose, as a fucose donor in the construction of fucosylated oligosaccharides. (hmdb.ca)
  • Functional studies of these fucose binding modules reveal binding to fucosylated oligosaccharides and suggest the importance of multivalent binding. (rcsb.org)
  • 2) The fucose bonding position shows the highest binding for oligosaccharides are the ones containing L-Fuc α1,6 and α1,2. (tcichemicals.com)
  • When in-vivo transformations of the fucose to oligosaccharides take place, such reactions are recognized as important indications of the antigen epitopes for the Lewis blood-type and cancer related carbohydrate antigens. (tcichemicals.com)
  • 4) The Aspergillus oryzae fucose specific lectin is not only utilized as an analyzing tool for the sugar-binding specificity of complex-type oligosaccharides, but it is highly applicable for a wide spectrum of studies on oligosaccharides. (tcichemicals.com)
  • We determined the specificity of the lectin towards l-fucose and fucosylated oligosaccharides, including alpha 1-6 linked core-fucose, which is an important marker for cancerogenesis. (archives-ouvertes.fr)
  • Recent studies have revealed that fucose removal from the oligosaccharides of human IgG1 antibodies results in a significant enhancement of antibody-dependent cellular cytotoxicity (ADCC) via improved IgG1 binding to FcγRIIIa. (aacrjournals.org)
  • In previous studies, we have shown that fucose is the most critical antibody oligosaccharide component and that the removal of fucose from IgG1 oligosaccharides results in a very significant enhancement of both ADCC in vitro (∼100 fold) and antitumor activity in vivo (17, 18) . (aacrjournals.org)
  • This study employed a commercial β-galactosidase in the production of fucose-containing galacto-oligosaccharides (fGOS) from lactose and fucose. (deepdyve.com)
  • L-fucose is a rare monosaccharide with limited natural availability and a precursor of high added-value complex carbohydrates, such as Human Milk Oligosaccharides (HMOs). (73100.pt)
  • Digitalose, the methyl ether of D-fucose Fucitol Verotoxin-producing Escherichia coli Garcia-Vaquero, M. (wikipedia.org)
  • Although fucose catabolic pathways have been enzymatically evaluated, the effects of fucose as a carbon source on intracellular metabolism in industrial microorganisms such as Escherichia coli are still not identified. (mdpi.com)
  • Kim, K.H. Metabolomic and Transcriptomic Analyses of Escherichia coli for Efficient Fermentation of L-Fucose. (mdpi.com)
  • Metabolism of L-fucose and L-rhamnose in Escherichia coli: aerobic-anaerobic regulation of L-lactaldehyde dissimilation. (asm.org)
  • A recombinant Escherichia coli strain was developed to produce guanosine 5′-diphosphate (GDP)- l -fucose, donor of l -fucose, which is an essential substrate for the synthesis of fucosyloligosaccharides. (springer.com)
  • The immunomodulating effects of fucose on the intestinal epithelial cells (IECs) towards the enteropathogen, Escherichia coli O157: H7 (E. coli O157) and/ or the probiotic Lactobacillus casei Shirota (LcS) were investigated. (nus.edu.sg)
  • l-Fucose Stimulates Utilization of d-Ribose by Escherichia coli " by Steven M. Autieri, Jeremy J. Lins et al. (uri.edu)
  • This subpathway is part of the pathway GDP-L-fucose biosynthesis via de novo pathway, which is itself part of Nucleotide-sugar biosynthesis. (uniprot.org)
  • View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose , the pathway GDP-L-fucose biosynthesis via de novo pathway and in Nucleotide-sugar biosynthesis . (uniprot.org)
  • GDP- d -mannose-4, 6-dehydratase (GMD) and GDP-4-keto-6-deoxymannose 3, 5-epimerase 4-reductase (WcaG), the two crucial enzymes for the de novo GDP- l -fucose biosynthesis, were overexpressed in recombinant E. coli by constructing inducible overexpression vectors. (springer.com)
  • Becker DJ, Lowe JB (2003) Fucose: biosynthesis and biological function in mammals. (springer.com)
  • GDP- l -fucose, the key substrate for fucosyloligosaccharide biosynthesis, has been synthesized via a de novo pathway in bacteria. (springeropen.com)
  • In the present study, genes for GDP- l -fucose biosynthesis were cloned into the expression vector pET-28a (+) to construct five E. coli strains, with recombinant enzymes being purified by using Ni-NTA chromatography. (springeropen.com)
  • We found that intrarenal expression of CL-11 rapidly increases in the postischemic period and colocalizes with complement deposited along the basolateral surface of the proximal renal tubule in association with L-fucose, the potential binding ligand for CL-11. (jci.org)
  • The high resolution crystal structures of ligand bound forms of one fucose binding module uncovers the molecular basis of fucose, ABH blood group antigen, and Lewisy antigen binding. (rcsb.org)
  • The fucose-mannose ligand-ELISA in the diagnosis and prognosis of canine visceral leishmaniasis in Brazil. (ajtmh.org)
  • The fucose-mannose ligand (FML)-ELISA assay showed a sensitivity of 100% and a specificity of 100% in diagnosis of canine visceral leishmaniasis (CVL) (kala-azar) in sera from naturally infected dogs from São Gonçalo do Amaranto, Rio Grande de Norte, Brazil. (ajtmh.org)
  • The fucose-mannose ligand (FML) of Leishmania infantum is a complex glycoprotein which does not elicit adequate immunogenicity in humans. (biomedcentral.com)
  • Among the various antigens that serve as targets for VL vaccine design, fucose-mannose ligand (FML) has attracted much attention owing to its excellent immunoprotective properties against experimental VL in several animal models [ 13 ]. (biomedcentral.com)
  • Here, we show that the O-fucose glycan in the Notch1 ligand-binding domain regulates the strength of Notch1 signaling during embryogenesis, post-weaning growth, and T cell development in the mouse. (elsevier.com)
  • Ge, C & Stanley, P 2008, ' The O-fucose glycan in the ligand-binding domain of Notch1 regulates embryogenesis and T cell development ', Proceedings of the National Academy of Sciences of the United States of America , vol. 105, no. 5, pp. 1539-1544. (elsevier.com)
  • Cytokine Profile of Leishmania Infantum Fucose-Mannose Ligand in Vaccinated Dogs in the Northwest of Iran', Iranian Journal of Immunology , 14(4), pp. 293-305. (ac.ir)
  • Few studies have confirmed that the fucose-mannose ligand (FML) antigen of Leishmania donovani produced protective immunity in dogs against CVL. (ac.ir)
  • In enzymology, a L-fucose isomerase (EC 5.3.1.25) is an enzyme that catalyzes the chemical reaction L-fucose ⇌ {\displaystyle \rightleftharpoons } L-fuculose Hence, this enzyme has one substrate, L-fucose, and one product, L-fuculose. (wikipedia.org)
  • Fucosylated glycans are constructed by fucosyltransferases, which require the substrate GDP-fucose. (nih.gov)
  • We propose that substrate specificity of 2-keto-3-deoxysugar acid aldolases was convergently acquired during evolution and report the discovery of another L-2-keto-3-deoxyfuconate aldolase involved in the same non-phosphorylating L-fucose pathway in Campylobacter jejuni . (news-medical.net)
  • The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-β-L-fucose as a donor substrate and transfers the fucose in O linkage to the protein producing fucose-α-O-serine/threonine. (morebooks.de)
  • GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. (nih.gov)
  • To this effect, designed optimized extraction of fucose-containing sulfated polysaccharides (FCSPs) and/or crude fucoidan from brown seaweed were performed, and the bioactivity of the isolated FCSPs was investigated. (dtu.dk)
  • In coastal regions brown algae are the most abundant biomass producers and their cell walls have fucose-containing sulfated polysaccharides (FCSP), known as fucans and/or fucoidans. (ua.pt)
  • Fucoidans are a group of fucose-containing sulfated polysaccharides (FCSPs) comprising mixtures of structurally related polysaccharides with certain variations of monosaccharide residues and containing non-carbohydrate substituents (mainly sulfate and acetyl groups) [ 3 ]. (mdpi.com)
  • Enzymatic synthesis of l-fucose from l-fuculose using a fucose isomerase fromRaoultellasp. (rcsb.org)
  • Studies of FUT8 and its enzymatic product, core fucose, are becoming an emerging area of interest in various fields of research including inflammation, cancer and therapeutics. (portlandpress.com)
  • 2015 ). As far as we know, there is no report on cell-free enzymatic synthesis of GDP- l -fucose from mannose. (springeropen.com)
  • After improving protein yield and purifying enzymes, we optimized enzymatic synthesis of GDP- l -fucose. (springeropen.com)
  • Two pathways for the synthesis of GDP-fucose operate in mammalian cells, the GDP-mannose-dependent de novo pathway and the free fucose-dependent salvage pathway. (nih.gov)
  • Albermann C, Distler J, Piepersberg W (2000) Preparative synthesis of GDP-β- l -fucose by recombinant enzymes from enterobacterial sources. (springer.com)
  • Synthesis and secretion appear to be facilitated in the presence of these sugars, although secretion of myeloma protein devoid of terminal fucose residues is possible for a limited time-period. (biochemj.org)
  • Watanabe, S. (2020) Characterization of L-2-keto-3-deoxyfuconate aldolases in a nonphosphorylating L-fucose metabolism pathway in anaerobic bacteria. (news-medical.net)
  • To elucidate the effects of fucose on cellular metabolism and to find clues for efficient conversion of fucose into bio-based products, comparative metabolomic and transcriptomic analyses were performed on E. coli on L-fucose and on D-glucose as a control. (mdpi.com)
  • Coffey JW, Miller ON, Sellinger OZ (1964) The metabolism of l -fucose in the rat. (springer.com)
  • It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. (nih.gov)
  • Here, we investigated the effect of the monosaccharide fucose on inflammation, metabolism, intestinal microbiota, and social behavior in the Dextran Sulfate Sodium (DSS)-induced chronic colitis mouse model. (nsu.ru)
  • The monosaccharide L-Fucose is a key component of important glycan structures found on extracellular mucoproteins and glycoproteins, in breast milk, and in certain blood-group determinants. (jennewein-biotech.de)
  • Fucose is the fundamental sub-unit of the seaweed polysaccharide fucoidan. (wikipedia.org)
  • Daniel, "Differentiation of the fucoidan sulfated L-fucose isomers constituents by CE-ESIMS and molecular modeling," Carbohydrate Research, vol. (thefreedictionary.com)
  • Fucoidan, which is extracted from brown seaweed, is a complex sulphated polysaccharide that is mostly composed of L-fucose and sulphated ester groups. (thefreedictionary.com)
  • The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan. (mdpi.com)
  • The monoclonal antibody, CCRC-M1, which recognizes a fucose (Fuc)-containing epitope found principally in the cell wall polysaccharide xyloglucan, was used to determine the distribution of this epitope throughout the mur1 mutant of Arabidopsis. (plantphysiol.org)
  • Up-regulation of an activation marker, CD69, on NK cells, particularly the CD56 dim subset, in the presence of both the antibody and target cells was much greater for the low-fucose antibodies. (aacrjournals.org)
  • However, many therapeutic antibodies currently approved or under clinical development are produced using Chinese hamster ovary cells that express high level of α1,6-fucosyltransferase and consequently produce low amounts antibody lacking fucose (17) . (aacrjournals.org)
  • This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1,3. (agrisera.com)
  • The antibody does not recognize alpha 1,6-fucose. (agrisera.com)
  • This suggests this antibody recognizes only α1,3 fucose epitopes. (agrisera.com)
  • We further investigated IgG1 binding to natural killer (NK) cells and NK cell activation during ADCC induction to elucidate the mechanism by which low-fucose IgG1 induces ADCC upon target cells with low antigen expression. (aacrjournals.org)
  • In addition, the chronic inhibition of myo-inositol transport with L-fucose induces a neuropathy that resembles diabetic neuropathy resulting in decreased nerve conduction velocity, axonal atrophy, paranodal swelling, and demyelination. (thefreedictionary.com)
  • Our previous studies indicated that 70% of Fut8-deficient (Fut8(-/-)) mice that lack core fucose structure die within 3 days after birth, but the remainder survive for up to several weeks although they show growth retardation as well as emphysema. (sigmaaldrich.com)
  • In this article, we focus on a glycosyltransferase, α1,6-fucosyltransferase (Fut8) and its product, the core fucose structure on N -glycans, and summarize the potential protective functions of this structure against emphysema and chronic obstructive pulmonary disease (COPD). (portlandpress.com)
  • FUT8 attaches a fucose residue onto N -linked-type complex glycoproteins ( N -glycans) [ 8 ], is expressed ubiquitously in mammalian tissues and is the sole enzyme responsible for generating a core fucose structure. (portlandpress.com)
  • The core fucose structure has a variety of functions that have been demonstrated in studies of Fut8 knockout mice ( Fut8 −/− ). (portlandpress.com)
  • Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction. (uniprot.org)
  • This protein is involved in step 2 of the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose. (uniprot.org)
  • 2004 ). The de novo pathway in bacteria, mammals and plants, starts from d -mannose conversion into GDP- l -fucose via a 5-step reaction (Fig. 1 ) (Chin et al. (springeropen.com)
  • GDP- d -mannose is processed to GDP- l -fucose in two additional steps: GDP- d -mannose-4,6-dehydratase (Gmd, EC 4.2.1.47) converts GDP- d -mannose to GDP-4-keto-6-deoxymannose and GDP-4-keto-6-deoxymannose-3, 5-epimerase-4-reductase (WcaG, EC 1.1.1.271) produces the end product, GDP- l -fucose. (springeropen.com)
  • Based on this well-studied pathway, many fermentation processes produce GDP- l -fucose using inexpensive and readily available mannose, have been developed. (springeropen.com)
  • recombinant Avidin expressed in corn contains α1,3 Fucose residues. (agrisera.com)
  • In the present study, we sought to evaluate the protective effects of a newly developed fucose-deficient recombinant antithrombin. (ijhgateway.net)
  • Here, screening of a library of 2-keto-3-deoxysugar acids with this protein and the biochemical characterization of neighboring genes revealed that this gene cluster encodes enzymes in a previously unknown 'Route I' non-phosphorylating L-fucose pathway. (news-medical.net)
  • Enzymes known as fucosidases split the fucose radical from polysaccharide molecules. (thefreedictionary.com)
  • Homologues for enzymes involved in a fucose salvage pathway are apparently absent in the P. falciparum genome. (conicet.gov.ar)
  • Two structural features distinguish fucose from other six-carbon sugars present in mammals: the lack of a hydroxyl group on the carbon at the 6-position (C-6) (thereby making it a deoxy sugar) and the L-configuration. (wikipedia.org)
  • In the fucose-containing glycan structures, fucosylated glycans, fucose can exist as a terminal modification or serve as an attachment point for adding other sugars. (wikipedia.org)
  • L-Fucose, one of the major monomeric sugars in brown algae, possesses high potential for use in the large-scale production of bio-based products. (mdpi.com)
  • GDP-fucose protein O-fucosyltransferase 1 (POFUT1) is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues between the second and third conserved cysteines in EGF-like repeats on the Notch protein. (morebooks.de)
  • L-fucose is a methyl pentose sugar which belongs to the family of deoxy sugars. (73100.pt)
  • GDP- l -fucose is one of the most expensive and rare nucleotide sugars. (springeropen.com)
  • The systematic name of this enzyme class is L-fucose aldose-ketose-isomerase. (wikipedia.org)
  • 6K1G: Crystal structure of the L-fucose isomerase soaked with Mn2+ from Raoultella sp. (rcsb.org)
  • Here, we performed biochemical and structural analyses of l-fucose isomerase identified from a novel species of Raoultella ( Rd FucI). (rcsb.org)
  • L-Lactaldehyde is a branching point in the metabolic pathway of L-fucose and L-rhamnose utilization. (asm.org)
  • Functions as a salvage pathway to reutilize L- fucose arising from the turnover of glycoproteins and glycolipids. (mybiosource.com)
  • De novo biosynthetic pathway of GDP- l -fucose. (springeropen.com)
  • l-Fucose has several potential applications in cosmetics, pharmaceuticals, and dietary supplements Fucosylation of antibodies has been established to reduce binding to the Fc receptor of Natural Killer cells and thereby reduce antigen-dependent cellular cytotoxicity. (wikipedia.org)
  • In this report, we investigated the relationship between enhanced ADCC and antigen density on target cells using IgG1 antibodies with reduced fucose. (aacrjournals.org)
  • Using EL4 cell-derived transfectants with differential expression levels of exogenous human CC chemokine receptor 4 or human CD20 as target cells, ADCC of fucose variants of chimeric IgG1 antibodies specific for these antigens were measured. (aacrjournals.org)
  • The Anti-fucose antibodies exhibit a positive response with PLA2 and Avidin and a negative one with asialofetuin. (agrisera.com)
  • Dot blot reaction of anti-Fucose and anti-Xylose antibodies with various controls: Avidin (Fuc+/Xyl+), Fetuin (Fuc-/Xyl-), PLA2 (Fuc+/Xyl-) and Mur1-2 (Fuc-/Xyl+). (agrisera.com)
  • Thus this may suggest that fucose is an immuno-modulator and the molar ratio between the bacteria and free fucose molecules determines the immune responses elicited. (nus.edu.sg)
  • Assay of urinary free fucose by fluorescence labeling and high-performance liquid chromatography. (semanticscholar.org)
  • Below are the list of possible Fucose-1-phosphate guanylyltransferase products. (mybiosource.com)
  • Also known as Fucose-1-phosphate guanylyltransferase (GDP-L-fucose diphosphorylase) (GDP-L-fucose pyrophosphorylase). (mybiosource.com)
  • I also suspect that your 1LB residues should actually be named 0LB (this might also be the case for your fucose residue). (vanderbilt.edu)
  • It was found to consist of disaccharide repeating units containing one of each 3-substituted residues of d -fucose and d - threo -pent-2-ulose (xylulose). (springer.com)
  • Heavy chains of the secreted form of the myeloma protein carry 90% of the label in fucose residues of their carbohydrate moieties. (biochemj.org)
  • Phospholipase A2 from honey bee venom (Apis mellifera) contains α1,3 Fucose residues. (agrisera.com)
  • In this review, we focus on the biological functions of mammalian fucosylated glycans and the biosynthetic processes leading to formation of the fucosylated glycan precursor GDP-fucose. (nih.gov)
  • with E. coli being used to overexpress GDP- l -fucose biosynthetic genes and Corynebacterium sp. (springeropen.com)
  • The α(1→3) linked core of fucose is a suspected carbohydrate antigen for IgE-mediated allergy. (wikipedia.org)
  • Members of the FTL family are characterized by a fucose recognition domain [F-type lectin domain (FTLD)] that displays a novel jellyroll fold ("F-type" fold) and unique carbohydrate- and calcium-binding sequence motifs. (frontiersin.org)
  • Growing attention has focused on the function of unusual O-linked carbohydrate modifications such as O-fucose. (semanticscholar.org)
  • The molecular weight of L-fucose specific lectin subunit, a dimeric substance, showed 35,000 (Fig. 1). (tcichemicals.com)
  • Low-fucose IgG1s showed potent ADCC at low antigen densities at which their corresponding high-fucose counterparts could not induce measurable ADCC. (aacrjournals.org)
  • The quantitative analysis revealed that fucose depletion could reduce the antigen amount on target cells required for constant degrees of ADCC induction by 10-fold for CC chemokine receptor 4 and 3-fold for CD20. (aacrjournals.org)
  • IgG1 binding to NK cells was increased by ligating IgG1 with clustered antigen, especially for low-fucose IgG1. (aacrjournals.org)
  • Our data showed that fucose removal from IgG1 could reduce the antigen amount required for ADCC induction via efficient recruitment and activation of NK cells. (aacrjournals.org)
  • 8488966 ). Elevated levels of serum fucose have been reported in breast cancer, ovarian cancer, lung cancer, liver cancer, diabetes and cardiovascular disease. (hmdb.ca)
  • Serum in the incubation medium supplies low-molecular-weight diffusible substances necessary to maintain continuous secretion of fucose-labelled myeloma protein beyond 2-3h, and of leucine-labelled myeloma protein beyond 6-8h. (biochemj.org)
  • Significance of Serum L-fucose Glycoprotein as Cancer Biomarker in Head and Neck Malignancies without Distant Metastasis. (semanticscholar.org)
  • Background: The objective of this study was to evaluate the serum and salivary L-fucose in oral potentially malignant disorders (OPMDs) and oral cancer (OC) in order to investigate the possibility of using this as biomarker for early diagnosis. (who.int)
  • Serum and unstimulated whole saliva were collected from participants of all groups and fucose estimation was done using spectrophotometry. (who.int)
  • 0.001) in serum and salivary L-fucose was observed in the study participants compared to control. (who.int)
  • Conclusions: The present study showed a significant and gradual increase in serum and salivary L-fucose from control to OPMDs to OC. (who.int)
  • Serum fucose level in malignant diseases. (bvsalud.org)
  • We review the present knowledge of serum fucose with special attention to its relation with various malignant diseases . (bvsalud.org)
  • We summarize the role of serum fucose as a useful diagnostic and prognostic marker when used singly or in combination. (bvsalud.org)
  • The purpose of this review is to provide an expert opinion on the practical and applied aspect of serum fucose level in clinical practice and research settings. (bvsalud.org)
  • We propose fucose as a promising prebiotic, since it creates a favorable environment for the beneficial bacteria that promote normalization of serum tryptophan level and amelioration of the behavioral abnormalities in the odor preference test. (nsu.ru)
  • Thus, the GDP-fucose precursor is essential in a wide variety of organisms. (conicet.gov.ar)
  • The Streptococcus pneumoniae fucose utilization operon includes a gene encoding a virulence factor that belongs to family 98 in the glycoside hydrolase classification. (rcsb.org)
  • Indeed, in vitro growth experiments showed that l-fucose stimulated utilization of d-ribose by the E. coli MG1655 Δ fucAO mutant but not by an E. coli MG1655 Δ fucK mutant. (uri.edu)
  • Furthermore, l-fucose at a concentration too low to support growth stimulated the utilization of ribose by the wild-type E. coli strains in vitro. (uri.edu)
  • and differing glycosidic linkages, and are variously substituted with sulfate and acetyl groups and side branches containing fucose or other glycosyl units. (dtu.dk)
  • 3H-fucose incorporation into RPE cell surface proteins of normal and dystrophic rats. (arvojournals.org)
  • Relative incorporation of 3H-fucose into high molecular weight cell surface glycoproteins (Mr of 128,000-183,000) was measured by quantitative autoradiography and densitometry. (arvojournals.org)
  • The results of these experiments show that 3H-fucose incorporation into four glycoproteins (Mr of 183,000, 175,000, 164,000 and 156,000) was reduced by 30-50% in the dystrophic RPE as compared to the normal cells. (arvojournals.org)
  • Incorporation of radioactive fucose into the immunoglobulin G1 myeloma protein secreted by mouse plasma-cell tumour MOPC 21 is stereospecific for the l -isomer. (biochemj.org)
  • Radioautographic and scintillation counting procedures were used to examine the effect of parathyroid hormone (PTH), dibutyryl cyclic-AMP (DB-cAMP), and colchicine on the incorporation of 3 H-fucose into macromolecular material in organ cultures of bone. (elsevier.com)
  • Radioautography demonstrated 3 H-fucose incorporation into bone cells, with the heaviest uptake occurring in osteoclasts. (elsevier.com)
  • By contrast, scintillation counting results indicated that PTH had little or no effect on 3 H-fucose incorporation, while DB-cAMP and colchicine considerably reduced the amount of labeled macromolecular material. (elsevier.com)
  • The incorporation of 3 H-fucose into glycoproteins and the role of glycoproteins are discussed. (elsevier.com)
  • Severson, AR 1981, ' 3H-fucose incorporation into bone following exposure to parathyroid hormone, dibutyryl cyclic-AMP and colchicine ', Cell and Tissue Research , vol. 214, no. 3, pp. 583-591. (elsevier.com)
  • By contrast, scintillation counting results indicated that PTH had little or no effect on 3H-fucose incorporation, while DB-cAMP and colchicine considerably reduced the amount of labeled macromolecular material. (elsevier.com)
  • Koizumi S, Endo T, Tabata K, Nagano H, Ohnishi J, Ozaki A (2000) Large-scale production of GDP-fucose and Lewis X by bacterial coupling. (springer.com)
  • The addition of fucose to the test animals' diet altered the bacterial community, increased the abundance of tryptophan-producing E. coli, normalized blood tryptophan levels, and ameliorated social behavior deficits. (nsu.ru)
  • 2017). Establishment of a tobacco BY2 cell line devoid of plant specific xylose and fucose as a platform for the production of biotherapeutic proteins. (agrisera.com)
  • Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250. (agrisera.com)
  • Glycoproteins were metabolically labeled with 3H-fucose in cultured RPE cells from RCS rdy-p+ and Long Evans rats. (arvojournals.org)
  • The absence of core fucose up-regulates GnT-III and Wnt target genes: a possible mechanism for an adaptive response in terms of glycan function. (sigmaaldrich.com)
  • These data suggest that Fut8(-/-) mice adapted to oxidative stress, both ex vivo and in vivo, by inducing various genes including GnT-III, which may compensate for the loss of core fucose functions. (sigmaaldrich.com)
  • One approach to increase GDP- l -fucose production is to overexpress GDP- l -fucose genes and optimize fermentation (Koizumi et al. (springeropen.com)
  • The specificity of lectin binding was tested by addition of free L-fucose to the AAL solution before addition to the microtiter plate. (thefreedictionary.com)
  • Jennewein Biotechnologie has successfully developed an environmentally friendly biotechnology-based production process that no longer requires the extraction of L-Fucose from algae and the resulting destruction of kelp forests. (jennewein-biotech.de)
  • The L-Fucose produced by Jennewein Biotechnologie is a crystalline product with high purity. (jennewein-biotech.de)
  • Fucose is a deoxyhexose that is present in a wide variety of organisms. (nih.gov)
  • When fucose was the carbon source for E. coli , integration of the two omics analyses revealed that excess gluconeogenesis and quorum sensing led to severe depletion of ATP, resulting in accumulation and export of fucose extracellularly. (mdpi.com)
  • These omics data and their biological interpretation could be used to assist metabolic engineering of E. coli producing bio-based products using fucose-containing brown macroalgae. (mdpi.com)
  • However, when the cells were incubated in fucose with 108 of E. coli O157 or LcS, the magnitude of immune responses observed were reduced. (nus.edu.sg)
  • The reverse was seen when the total concentration of the bacteria was doubled (a mixture of 108 of E. coli O157 and 108 LcS) in the presence of fucose. (nus.edu.sg)
  • To determine the roles of l-fucose and d-ribose, an E. coli MG1655 Δ fucAO mutant and an E. coli MG1655 Δ rbsK mutant were fed separately to mice along with wild-type E. coli MG1655. (uri.edu)
  • and E. coli are the primary producers of GDP- l -fucose. (springeropen.com)
  • This protein contains a C-terminal triplet of fucose binding modules that have significant amino acid sequence identity with the Anguilla anguilla fucolectin. (rcsb.org)
  • O-fucose modifications have been described in several different protein contexts, including epidermal growth factor-like repeats and thrombospondin type 1 repeats. (semanticscholar.org)
  • Clinical value of protein-bound fucose in patients with carcinoma and other diseases. (semanticscholar.org)
  • Protein-bound fucose content in sera from normal persons and patients with various malignant and non-malignant diseases was measured and statistically analyzed. (semanticscholar.org)
  • Maximum GDP- l -fucose concentration of 38.9 ± 0.6 mg l −1 was obtained in a glucose-limited fed-batch cultivation, and it was enhanced further by co-expression of NADPH-regenerating glucose-6-phosphate dehydrogenase encoded by the zwf gene to achieve 55.2 ± 0.5 mg l −1 GDP- l -fucose under the same cultivation condition. (springer.com)
  • Involved in GDP-fucose import from the cytoplasm into the Golgi lumen. (nih.gov)
  • This gene encodes a GDP-fucose transporter that is found in the Golgi apparatus. (nih.gov)
  • glutinins produce a heteropolysaccharide composed of L-fucose and Dgalactosein an approximate molar ratio of 1:1. (thefreedictionary.com)
  • In human N-linked glycans, fucose is most commonly linked α-1,6 to the reducing terminal β-N-acetylglucosamine. (wikipedia.org)
  • In mammals, fucose-containing glycans have important roles in blood transfusion reactions, selectin-mediated leukocyte-endothelial adhesion, host-microbe interactions, and numerous ontogenic events, including signaling events by the Notch receptor family. (nih.gov)
  • Fucose in N-glycans: from plant to man. (nih.gov)
  • This article discusses what we can learn from studies of Fut8 and core fucose by using knockout mice or in vitro studies that were conducted by our group as well as other groups. (portlandpress.com)
  • As a free sugar, L-fucose is normally found at very low levels in mammals. (hmdb.ca)
  • Thus, the O-fucose glycan in EGF12 of mouse Notch1 is required for optimal Notch1 signaling and T cell development in mammals. (elsevier.com)
  • Asialofetuin is a glycoprotein with α1,6 fucose. (agrisera.com)
  • Fucose is a hexose deoxy sugar with the chemical formula C6H12O5. (wikipedia.org)
  • Therefore, metabolic engineering and optimization are needed for E. coil to more efficiently ferment fucose. (mdpi.com)
  • This is in agreement with in vivo metabolic labeling experiments showing that fucose is not significantly incorporated by the parasite. (conicet.gov.ar)
  • Fucose is released from fucose-containing polymers by an enzyme called α-fucosidase found in lysosomes. (wikipedia.org)
  • Fucose polymers are synthesized by fucosyltransferases. (hmdb.ca)
  • Although differently extracted, purified, fucose-rich, modified fucoidans have been reported to exert bioactive properties such as anti-coagulant and enhance immune response activity, few studies have investigated the bioactivity of unfractionated FCSPs, notably FCSPs extracted using milder and fewer processing steps. (dtu.dk)
  • Fucose is metabolized by an enzyme called alpha-fucosidase. (hmdb.ca)