An abnormal hemoglobin resulting from the substitution of valine for glutamic acid at position 6 of the beta chain of the globin moiety. The heterozygous state results in sickle cell trait, the homozygous in sickle cell anemia.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
The rate dynamics in chemical or physical systems.
NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
Places for cultivation and harvesting of fish, particularly in sea waters. (from McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Large vessels propelled by power or sail used for transportation on rivers, seas, oceans, or other navigable waters. Boats are smaller vessels propelled by oars, paddles, sail, or power; they may or may not have a deck.
The practice of medicine concerned with conditions affecting the health of individuals associated with the marine environment.
Common name for various species of large, vigorous ocean fishes in the family Scombridae.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A body of water located at the southeastern corner of North America. It is bordered by the states to the north of Florida, Alabama, Mississippi, Louisiana, Texas; by five Mexican states to the west: Tamaulipas, Veracruz, Tabasco, Campeche, Yucatan; and by Cuba to the southeast.
An alcoholic beverage usually made from malted cereal grain (as barley), flavored with hops, and brewed by slow fermentation.
Salts that melt below 100 C. Their low VOLATILIZATION can be an advantage over volatile organic solvents.
Liquids transforming into solids by the removal of heat.
A continuing periodic change in displacement with respect to a fixed reference. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A gas that condenses under slight pressure. Because of its low boiling point ethyl chloride sprayed on skin produces an intense cold by evaporation. Cold blocks nerve conduction. Ethyl chloride has been used in surgery but is primarily used to relieve local pain in sports medicine.
A feeling of restlessness associated with increased motor activity. This may occur as a manifestation of nervous system drug toxicity or other conditions.
The act or practice of literary composition, the occupation of writer, or producing or engaging in literary work as a profession.
An activity in which the body advances at a slow to moderate pace by moving the feet in a coordinated fashion. This includes recreational walking, walking for fitness, and competitive race-walking.
Communication through a system of conventional vocal symbols.
The act, process, or result of passing from one place or position to another. It differs from LOCOMOTION in that locomotion is restricted to the passing of the whole body from one place to another, while movement encompasses both locomotion but also a change of the position of the whole body or any of its parts. Movement may be used with reference to humans, vertebrate and invertebrate animals, and microorganisms. Differentiate also from MOTOR ACTIVITY, movement associated with behavior.
Four or five slender jointed digits in humans and primates, attached to each HAND.
The process whereby an utterance is decoded into a representation in terms of linguistic units (sequences of phonetic segments which combine to form lexical and grammatical morphemes).
Area of the FRONTAL LOBE concerned with primary motor control located in the dorsal PRECENTRAL GYRUS immediately anterior to the central sulcus. It is comprised of three areas: the primary motor cortex located on the anterior paracentral lobule on the medial surface of the brain; the premotor cortex located anterior to the primary motor cortex; and the supplementary motor area located on the midline surface of the hemisphere anterior to the primary motor cortex.
Procedures or techniques used to keep food from spoiling.
A food group comprised of EDIBLE PLANTS or their parts.
Substances capable of inhibiting, retarding or arresting the process of fermentation, acidification or other deterioration of foods.
The pressure due to the weight of fluid.
Any substances taken in by the body that provide nourishment.
The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.
The production and movement of food items from point of origin to use or consumption.

Sex differences in the effects of early neocortical injury on neuronal size distribution of the medial geniculate nucleus in the rat are mediated by perinatal gonadal steroids. (1/2521)

Freezing injury to the cortical plate of rats induces cerebrocortical microgyria and, in males but not females, a shift toward greater numbers of small neurons in the medial geniculate nucleus (MGN). The purpose of the current study was to examine a hormonal basis for this sex difference. Cross-sectional neuronal areas of the MGN were measured in male rats, untreated female rats and female rats treated perinatally with testosterone propionate, all of which had received either neonatal cortical freezing or sham injury. Both male and androgenized female rats with microgyria had significantly smaller MGN neurons when compared to their sham-operated counterparts, whereas untreated females with microgyria did not. These differences were also reflected in MGN neuronal size distribution: both male and androgenized female rats with microgyria had more small and fewer large neurons in their MGN in comparison to shams, while there was no difference in MGN neuronal size distribution between lesioned and sham females. These findings suggest that perinatal gonadal steroids mediate the sex difference in thalamic response to induction of microgyria in the rat cortex.  (+info)

Improved antibody detection by the use of range expansion and longer filter wavelength in a low ionic strength-protamine sulphate Auto-Analyzer system. (2/2521)

Range expansion, achieved by insertion of a variable resistance between the colorimeter and the recorder together with the use of 550 nm colorimeter filters, has resulted in markedly improved sensitivity for antibody detection, and improved sample identification, in a low ionic strength-protamine sulphate (LISPS) system. Range expansion also permits a lower concentration of red cells to be used, thus economizing on fully typed cells. Glycerol stored frozen cells were found to be only slightly less sensitive than fresh cells in this system.  (+info)

Cerebral blood flow in the monkey after focal cryogenic injury. (3/2521)

A focal cryogenic lesion was made in the left superior frontal gyrus of the anesthetized macaque brain. Cerebral blood flow (CBF) was determined by the hydrogen clearance technique before and during the 4 hours following trauma. Local CBF in tissue adjacent to the lesion increased in the first half hour after the lesion was made and then decreased during the ensuing 3 1/2 hours. Local CBF in the contralateral superior frontal gyrus, as well as total CBF and oxygen consumption, were unchanged by cryogenic trauma. The spread of vasogenic edema into uninjured tissue probably accounts for the observed decrease in local CBF. This experimental model may assist in discovering therapy to alter favorably the spatial and temporal profile of pathologic CBF changes in tissue surrounding an acute lesion of the brain.  (+info)

Preferential acid-catalyzed hydrolysis of the formamide linkage of N'-formylkynurenine in frozen solution. (4/2521)

Acid-catalyzed hydrolysis of the formamide linkage of N-acetyl-N'-formyl-L-kynurenineamide in frozen dilute hydrochloric acid solution followed first-order kinetics, yielding N-acetyl-L-kynurenineamide as the sole reaction product. The maximum rate of reaction in the frozen solution was found at around -7.5 degrees and approximated that of the reaction in liquid solution at 40 degrees. By freezing the dilute acid solution at -8 degrees the reaction was accelerated by 60 times compared with that in super-cooled liquid solution at the same temperature.  (+info)

A bioluminescence method for the mapping of local ATP concentrations within the arterial wall, with potential to assess the in vivo situation. (5/2521)

According to the anoxemia theory of atherosclerosis, an imbalance between the demand for and supply of oxygen and nutrients in the arterial wall is a key factor in atherogenesis. However, the energy metabolic state of the arterial tissue in vivo is largely unknown. We applied a bioluminescence method, metabolic imaging, to study local ATP concentrations in cryosections of normal pig and atherosclerotic and normal rabbit aorta. Some vessels were subjected to energy metabolic restrictions by incubation at different oxygen and glucose concentrations and others were rapidly frozen in liquid nitrogen to reflect the in vivo situation. Local ATP concentrations and the ATP distribution at a microscale was dependent on oxygen as well as glucose concentrations during incubation. ATP depletion was seen in the mid media of pig aorta in all incubations, but only at low oxygen concentration without glucose in the media of the thinner rabbit aorta. ATP-depleted zones were seen deep in pig media (>750 microm from the lumen) and in rabbit plaques (>300 micrometer+ from the lumen) even at high oxygen (pig 75% O2 and rabbit 21% O2) and glucose concentrations (5.6 mmol/L glucose). This observation probably illustrates an insufficient diffusion of glucose, which highlights the importance of studying the conditions for diffusion not only of oxygen but also of other metabolites in the arterial wall. In rapidly frozen vessels the medial ATP concentration was shown to be 0.6 to 0.8 micromol/g wet weight (both pig and rabbit aorta) and in pig aorta a gradient could be seen indicating higher ATP concentrations at the lumenal side. We propose that metabolic imaging, as applied to snap-frozen tissue, may be used to assess the energy metabolic situation in the arterial wall in vivo. The spatial resolution allows the detection of local variations within the arterial tree. However, steep concentration gradients (eg, near the border of the tissue) will be underestimated. The method may be extended to include determinations of glucose and lactate concentrations and will be used in parallel with an established method to assess hypoxia in the arterial wall in vivo.  (+info)

Changes in allograft bone irradiated at different temperatures. (6/2521)

Secondary sterilisation of allograft bone by gamma irradiation is common, but the conditions under which it is performed vary between tissue banks. Some do so at room temperature, others while the bone is frozen. Bone is made brittle by irradiation because of the destruction of collagen alpha chains, probably mediated by free radicals generated from water molecules. Freezing reduces the mobility of water molecules and may therefore decrease the production of free radicals. We found that bone irradiated at -78 degrees C was less brittle and had less collagen damage than when irradiated at room temperature. These findings may have implications for bone-banking.  (+info)

Rough surfaced smooth endoplasmic reticulum in rat and mouse cerebellar Purkinje cells visualized by quick-freezing techniques. (7/2521)

The in vivo structure of the smooth endoplasmic reticulum (ER) was visualized in rat and mouse cerebellar Purkinje cells by using quick-freezing techniques followed by freeze-substitution for ultrathin-sectioning or freeze-fracturing and deep-etching for replicas. High magnification electron microscopy of the ultrathin sections revealed a surprising finding that all the smooth ER are apparently rough surfaced, and heavily studded with a large number of small dense projections. In the soma the smooth ER appears to be similar to its rough counterpart, except that the projections are slightly smaller, less electron dense and less protrusive on the ER membranes than the ribosomes. The projections were short rectangles, 20 x 20 x 6 nm3 in size, covering the cytoplasmic surface of the smooth ER in a checker-board manner where closely packed. After freeze-etching and replication, they appeared to be composed of four subparticles, surrounding a central channel. Thus the projections are very similar to the foot structure (ryanodine receptor) of the sarcoplasmic reticulum. Furthermore, they were distributed exclusively in the ER compartment and were highly concentrated especially in the smooth ER. This localization of the projections coindides with the intracellular distribution of the inositol 1,4,5-trisphosphate (IP3) receptor determined by quantitative immunogold electron microscopy. These findings would suggest that the projections are tetramers of IP3 receptor molecules and could be used as a morphological marker for the smooth ER in Purkinje cells, which spreads from the soma to the axon and dendrite, up to the tips including the spines. In Purkinje cells tubular smooth ER runs freely in a serpentine fashion or are intertwined to make large membraneous tangles without forming cisternal stacks. It is highly probable that the ER cisternal stacks do not exist naturally in Purkinje cells but are formed artificially during the various procedures for chemical fixation.  (+info)

A leucine-rich repeat protein of carrot that exhibits antifreeze activity. (8/2521)

A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs.  (+info)

TY - JOUR. T1 - Effect of lysed platelet count in platelet concentrates on various growth factor levels after freeze thaw cycles. AU - Nugraha, Yudhi. AU - Sari, Pudji. AU - Purwoko, Reza Yuridian. AU - Luviah, Evah. AU - Adiwinata, Jeanne. PY - 2014/1/1. Y1 - 2014/1/1. N2 - Platelet concentrate (PC) has been used as substitute to the use of fetal bovine serum (FBS) in cell culture media. However, its use as additive in cell culture media showed inconsistent results on cell proliferation, and the inconsistent results may be due to variability of platelet counts or growth factor content. Standard protocols are lacking for the preparation of PC before it is applied as additive in cell culture media. The growth factor content of PC can be released by freeze-thaw cycles, which range from one to three cycles before use for cell culture media. This study aimed to measure base-line platelet counts and growth factor levels and compare to platelet counts and growth factor levels after one, two and three ...
Repeated freeze thaw cycles do not affect the ELISA response.One mL aliquots of either total Sloop hordein (1.0 mg/mL; in a solution containing 8 M urea, 1% (w/
en] The Km, app. values of the membrane-bound transpeptidase of Streptomyces R61 for the donor Ac2-L-Lys-D-Ala-D-Ala and the acceptor Gly-Gly are not affected by temperature variations when the reaction mixtures are incubated in liquid suspensions. At -5 degrees C, the incubation can be carried out either in the liquid or in the frozen state. The enzyme is active in the latter state. In the frozen state, the Km, app. value for the acceptor remains unchanged but there is a 3-fold increase in the maximum velocity, a 10-fold decrease of the Km, app. value for the donor and a 10-fold increase of the benzylpenicillin concentration required to inhibit the enzyme activity by 50% (ID50 value). Temperatures of -35 degrees C or below are required to completely inhibit the membrane-bound enzyme in the frozen state. Cetyltrimethylammonium bromide extracts the transpeptidase both from the isolated membranes and, with a much higher yield, from the intact mycelium. The extracted enzyme is not active in the ...
AAV Viruses are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles. ...
AAV Viruses are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles. ...
AIM: The effects on neural repair of intraparenchymal nerve growth factor (NGF) administration were evaluated in neonate Wistar rats with experimentally induced focal microgyria. METHODS: A freezing focal polymicrogyric lesion was induced on the frontal cortex in 35 newborn Wistar rats on postnatal day 1. NGF was administered in 15 cases, with 20 pups as controls. Animals were sacrificed at 72 h and 7 days after NGF administration. Real-time PCR was used for the quantification of the expression of TrkA, p75, and doublecortin (DCX) at the level of the cortical lesion in seven different groups of animals: control 72 h (n = 5), control 7 days (n = 5), microgyria 72 h (n = 5), microgyria 7 days (n = 5), microgyria + NGF 72 h (n = 5), microgyria + NGF 7 days (n = 5), and control + NGF (n = 5 ...
D7792 / D7792M-15 Standard Practice for Freeze/Thaw Conditioning of Pultruded Fiber Reinforced Polymer (FRP) Composites Used in Structural Designs test specimens~ freeze-thaw cycle~ structural design~ fiber reinforced polymer~
Back to Recommended Freeze-Thaw Risk Assessment Steps. In many projects, the risk is not clear from inspection alone. Material testing is the next step in evaluating risk. Samples are removed from the building being assessed and shipped to our lab.. Lab tests can include dry density, A-value (absorption or liquid water uptake rate), and saturation moisture content (storage). However, the primary method used by BSC to assess the risk of freeze-thaw damage is frost dilatometry. Frost dilatometry, as first developed by Fagerlund1, involves putting thin slices of brick through multiple freeze-thaw cycles until damage is measurable. This process produces a measure of Scrit (the Critical Degree of Saturation) - the point of saturation at which a given masonry material will fail. By estimating the typical degree of saturation for a building, and comparing it to Scrit, the risk of freeze-thaw damage can be predicted. Freeze-thaw cycling below Scrit has low risk of damage, but above Scrit, damage can ...
TY - JOUR. T1 - Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. AU - Teo, Ka Yaw. AU - DeHoyos, Tenok O.. AU - Dutton, J. Craig. AU - Grinnell, Frederick. AU - Han, Bumsoo. N1 - Funding Information: This research was supported by grants from the National Institute of Health / National Institute of Biomedical Imaging and Bioengineering , R01 EB008388 , and the National Science Foundation , CBET-1009465 . PY - 2011/8. Y1 - 2011/8. N2 - The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In ...
Centrifuge and separate serum/plasma from cells and transfer to plastic vials; do not freeze whole blood. ,Frozen -20°C up to 4 weeks (with up to 5 freeze thaw cycles) ,Frozen -20°C up to 6 months (with up to 1 freeze thaw cycle) ...
When you freeze aqueous solutions slowly you form larger ice crystals. This would segregate water away from proteins and into the crystals of ice. As the amount of unfrozen water drops the protein becomes more and more concentrated and loses the water shell required to maintain its structure (remember the primary force for protein structure is the organisation of hydrophobic and hydrophilic residues). This quickly leads to protein denaturation and aggregation, and is one of the reasons why freeze thaw cycles are so damaging to proteins. Proteins are frozen quickly to produce the smallest ice crystals possible as quickly as possible, which minimises the disruption described above. Formulation of the protein with excipients such as polyols and carbohydrates can also help dramatically improve stability during freeze thaw by helping to lower the total amount of water bound up in ice crystals, and to take the place of water at the proteins surface. This can allow you to freeze proteins more slowly ...
Freeze & Thaw technology on another quality level | mAbs | Single-use technologies are highly flexible & scalable - learn more about our process!
If i were to have a blind attempt at your question, it is never a good idea to freeze thaw any protein, not even cell lysates. Thats the whole point of glycerol use, which we use in our lab at 50% (v/v) to store bead bound proteins. Thats also why antibodies and restriction enzymes are stored in glycerol since freeze thawing antibodies dissolved in water often leads to their degradation, although you only tend to store stuff in glycerol, like restriction enzymes, if the concentration of the protein is very very high. One golden trick I use is aliquoting my samples and snap freeze them in liquid nitrogen and store in -80 freezer. Im curious as to what happens with your protein, does it degrade after a freeze thaw cycle? (Im aware that transmembrane proteins are notorious when it comes to storage/maintenance due to their awkward hydrophobic transmembrane region so its best to speak to an x-ray crystallography expert, if there is some one like that roaming around your research lab :), since they ...
1. Remove the 10X T4 DNA Ligase Reaction Buffer* from the freezer to thaw. You can remove the T4 DNA Ligase enzyme from the freezer at this point but leave the ligase in a cold box to keep it close to -20○ C. Thawing is fast if the buffer tube is immersed in room temperature water. Once thawed, agitate the 10X T4 DNA Ligase Reaction Buffer until all precipitate goes into solution. 2. Add 11ul of H2O to a 200ul PCR tube. 3. Add 2ul from each of the digest to the tube.** 4. Add 2ul of 10X T4 DNA Ligase Reaction Buffer to the tube. 5. Add 1ul of the T4 DNA Ligase to the tube. 6. The total volume in each tube should now be 20ul. Ensue the ligation is well mixed by flicking the tube. You can spin the tube in a microcentrifuge for a few seconds to collect the liquid in the bottom of the tube again. *Repeated freeze-thaw cycles of the buffer can degrade the ATP in the buffer thereby making the ligation reaction less efficient. It is wise to aliquot the buffer into 10ul aliquots prior to freezing ...
The shelf life for peptide in solution form is limited, especially for the peptides which contain Cysteine (C), Methionine (M), Tryptophan (W), Asparagine (N), Glutamine (Q) and N-Glutamic Acid (E) are prone to air oxidation. It is recommended to purge the air out of the vial and replace it with a blanket of nitrogen or argon. If storage in solution form is unavoidable, use sterile buffers around pH 5 to 6 and freeze aliquots for storage at -200oC to prolong the storage life of peptide in solution. Furthermore, avoid the use of frost-free freezer as there will be variations in temperature during the frequent defrosting cycle. Repeated freeze-thaw cycles can also damage the peptides. ...
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu EPO-alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles ...
Volume 1 ml (1 mg/ml) Description Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody Clonality | Host Species | Isotype GF28R | Mouse | IgG2b Immunogen GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates Specificity Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP Purification Protein A affinity chromatography from mouse ascites fluid Application Dot, ELISA, IP, IS, WB Sensitivity WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins Working Condition WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage Store at -20 C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 C (add 0.05 NaN3) for several days to weeks Images Fig. 1:1000 (1 g/mL)
Note : For neural progenitor cell expansion in monolayer, culture vessels have to be pre-coated with substrate for cell attachment. Either PRIME-XV Human Fibronectin or MatrIS F should be used as coating substrates. For consistent performance, usage of Poly-L-Ornithine is also recommended to be used in conjunction with PRIME-XV Human Fibronectin or PRIME-XV MatrIS F. Specific concentrations and types of substrates should be optimized for each end-user application. 1. Dissolve Poly-L-Ornithine in sterile PBS to make a 15 mg/mL stock (1000X). Aliquot and store at ≤-20˚C in a manual defrost freezer for up to 6 months. Avoid repeated freeze-thaw cycles.. 2. Dilute the 1000X Poly-L-Ornithine stock 1000-fold in sterile PBS to make a 15 µg/mL (1X) solution. Prepare fresh as needed.. 3. Add the (1X) Poly-L-Ornithine solution to culture vessel at a ratio of 0.15 mL/cm2. Incubate 3 hours to overnight at 37˚C and 5% CO2.. 4. Discard the Poly-L-Ornithine solution. Wash each vessel 3 times with equal ...
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution ...
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles ...
Methods: Analyses of HFABP were performed on an Advia 2400 (Siemens Healthcare Diagnostics). Imprecision, limit of detection (LOD), limit of blank (LOB), and linearity were assessed using standard methods. Stability was assessed at 4 °C, −20 °C, and with 3 repeated freeze-thaw cycles. Clinical diagnostic performance was assessed using chest pain in patients, with a final diagnosis according to the universal definition of myocardial infarction with cardiac troponin I (cTnI) measured on the Siemens Advia Centaur (cTnI Ultra method, 99th percentile 50 ng/L, 10% CV 30 ng/L). Ischemia was detected using sampling pre- and postangioplasty. ...
TIANCHI Small Liquid Nitrogen Tanks 3 Liter LN2 Dewar Container Cryo Tank. Net Weight(KG):3.2. Gross Weight(KG):5.0. Static Power Dissipation(Kg/d):0.08. This Small Liquid Nitrogen Tanks 3 Liter is made of high-strength, special corrosion resistant aluminum alloy, light weight and easy to carry. Liquid nitrogen storage containers is well made, very good insulation, integrated molding, weld the small, solid and reliable, guarantee vacuum and prevent leakage. Liquid nitrogen container low loss, more than 5 years of vacuum quality.. Liquid nitrogen storage tank can be stored for a long biological specimens, semen, embryos, spawn, vaccines, skin, organ and other biological material. Products three guarantees service, for each customer to establish user files, and enjoy lifelong technical services. This LN2 Dewar Container is only suitable for the static storage of liquid nitrogen, not suitable for the transport of liquid nitrogen. This cryogenic container ships empty. It can be filled anywhere that ...
PA5-27732 targets PKC gamma in WB applications and shows reactivity with Human samples. The PA5-27732 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 166 of Human PKC gamma. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles ...
Amino Acid SequenceMGSSHHHHHH SSGLVPRGSH MGSMPALHIE DLPEKEKLKM EVEQLRKEVK LQRQQVSKCS EEIKNYIEER SGEDPLVKGI PEDKNPFKEK GSC.DescriptionGNG11 Human Recombinant produced in E. coli is. a single polypeptide chain containing 93 amino acids (1-70) and having a molecular mass of 10.6kDa. GNG11 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.FormulationThe GNG11 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.Physical AppearanceSterile Filtered clear solution.PurityGreater than 90% as determined by SDS-PAGE.SourceEscherichia Coli.StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.SynonymsGNGT11, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11, GNG11.Linkhttp://www.prospecbio.com/GNG11_Human_10
White 2 Freezer Storage Boxes With 9 x 9 Grid from PGC Scientifics,Fiberboard boxes are ideal for use in refrigerators, freezers, or liquid nitrogen. Conveniently sized to fit various storage racks. Four vibrant colors allow for color coding to project, user, lab, etc. Holes in the bottom allow liquid nitrogen drainage or promote cool air circulation. Supplied wit,biological,biology supply,biology supplies,biology product
I live in Alaska. That means I am very well-acquainted with frost heaves, which are those nasty seasonal rises and depressions in what used to be smooth pavement. Caused by multiple freeze-thaw cycles in roadbeds prone to water retention, youre lucky if your vehicle doesnt bottom out on them, or worse, have the front bumper and rear bumper crash onto consecutive frost heaves, leaving it suspended in the air, or your vehicle bumper-less and your gas tank leaking ...
These are the general reagents used for all ligation reactions. Discussion of the specific reagents will be in each individual protocol. Sortase: A Sortase A construct with the N-terminal membrane targeting sequence removed is readily purified in N-terminally His-tagged form. The pETMCSIII based vector pLLC064 is available for expression of this construct. The protein is easily overexpresed and purified using standard nickel affinity chromatography conditions. It should be noted that the protein migrates anomalously on SDS-PAGE gels and appears to run as a protein of ~26 kDa molecular weight (###). We have confirmed the correct molecular weight of Sortase A expressed from pLLC064 by MS. The protein is very stable. We find it convenient to store the protein in Sortase buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, pH 7.5). The protein appears stable to multiple freeze-thaw cycles. We have not as yet investigated the potential to freeze dry Sortase for storage although we hope to carry out this ...
S-cysteinylated albumin and methionine-oxidized apolipoprotein A-I (apoA-I) have been posed as candidate markers of diseases associated with oxidative stress. Here, a dilute-and-shoot form of LC-electrospray ionization-MS requiring half a microliter of blood plasma was employed to simultaneously quantify the relative abundance of these oxidized proteoforms in samples stored at −80 °C, −20 °C, and room temperature and exposed to multiple freeze-thaw cycles and other adverse conditions in order to assess the possibility that protein oxidation may occur as a result of poor sample storage or handling. Samples from a healthy donor and a participant with poorly controlled type 2 diabetes .... ...
Do not mix reagents with those from other lots.. Reactivity : Bovine. Method type : Sandwich ELISA. Minimum Detection Limit :. Detection Range :. Application : ELISA. Purpose : For the quantitative determination of target substances concentrations.. Research Area : Immunology-,Innate Immunity-,Complement-,Alternative Pathway, Immunology-,Innate Immunity-,Complement-,Other. Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates. Plate : Pre-coated,Strips (12 x 8). Restrictions : For Research Use only. Storage : 2 °C - 8 °C. Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. Expiry Date : 12 months. Size : 96T. Uniprot No. :. Abbreviation :. Availability : 3-5 working days. Target Details :. Precision : Intra-assay Precision (Precision within an assay) CV%,15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays) CV%,15% Three samples of ...
Features More than purity Stable after multiple freeze-thaw cycles dNTPs triphospate form after weeks room temperature Perfect standard real-time high-fidelity 1-Step long range PCRBIO dNTP ultra-pure stable suitable variety applications PCRBIO dNTP been tested many molecular biology applications including standard fast 1-Step high-fidelity real-time long-range LAMP sequencing
Do not mix reagents with those from other lots.. Reactivity : Rat. Method type : Sandwich ELISA. Minimum Detection Limit : Detection Range : Application : ELISA. Purpose : For the quantitative determination of target substances concentrations.. Research Area : Immunology->Innate Immunity->Complement->Alternative Pathway, Immunology->Innate Immunity->Complement->Other. Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates. Plate : Pre-coated,Strips (12 x 8). Restrictions : For Research Use only. Storage : 2 °C - 8 °C. Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. Expiry Date : 12 months. Size : 96T. Uniprot No. : Abbreviation : Availability : 3-5 working days. Target Details : Precision : Intra-assay Precision (Precision within an assay) CV ...
A freeze-thaw valve is provided using a Peltier heat pump where the thermal short-circuit path between a cooled thermal mass and a heated thermal mass is reduced or absent and the valve state transition time is minimized. The freeze-thaw valve comprises a Peltier heat pump mounted to a heat exchange surface that comprises a cross-drilled copper water jacket or manifold. The Peltier heat pump is operated to maintain a cooled thermal mass at a substantially constant low temperature. A resistance heating element is used to produce a heated thermal mass. The freeze-thaw segment of a fluid conduit is commutated to contact either the heated or the cooled thermal mass to thaw and therefore open the valve or cool and thus close the valve. The operation of the Peltier heat pump at a constant temperature avoids problems inherent in the use of a Peltier heat pump to both heat and cool a freeze thaw segment.
Glycerol is also used to decrease the freezing point of protein solution which are easily degraded by repeated freeze/thaw cycles. A 50% (w/v) glycerol/water solution freezes at -25ºC and can thus be stored in a -20ºC freezer. Note that glycerol with 1.26g/cm3 is much denser than water and, therefore, a 50% (v/v) solution will be very different from a 50% (w/v) solution. For example, restriction enzymes are typically stored in a glycerol/water solution. ...
The lyophilized rMu VEGF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu VEGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.10% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze / thaw cycles ...
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-12 should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles ...
Ultra-low Temperature Freezer Storage Tank Report by Material, Application, and Geography Global Forecast to 2021 is a professional and comprehensive research report on the worlds major regional market conditions,
Ultra-low Temperature Freezer Storage Tank Report by Material, Application, and Geography Global Forecast to 2021 is a professional and comprehensive research report on the worlds major regional market conditions,
Chondrex, a major secretory protein of human chondrocytes and synovial cells, has been previously shown to be increased in the synovial fluid and serum of individuals with joint and cartilage disease (3)(6). We have developed a simple, fast, two-site, sandwich-type ELISA that uses a streptavidin-coated microplate well, a biotinylated-Fab monoclonal capture antibody, and an alkaline phosphatase-labeled polyclonal detection antibody. The assay involves three 1-h incubation steps, is carried out at room temperature, and does not require sample pretreatment.. The Chondrex assay has good within-run and between-run precision, good linearity upon dilution of samples, and good analytical recovery. Chondrex concentrations in serum samples are stable at 4 °C for as long as 9 days and as many as 6 freeze/thaw cycles. The measurement of chondrex in serum is not affected by abnormally high concentrations of common serum components such as bilirubin, hemoglobin, triglycerides, and total protein. In addition, ...
Some deciduous shrubs can benefit from extra care for winter months. While most deciduous shrubs are not susceptible to sun scald like deciduous trees are, those with larger central stems or trunks exposed to full sun may benefit from wrapping. Newly planted shrubs may also benefit from extra mulch over the root zone to prevent damage from the freeze/thaw cycles of late winter, which can damage roots. Salt used to melt ice on pavement can damage shrubs in two ways. Shrubs planted near roadways often suffer from salt spray damage, resulting in bud death and twig dieback. New growth then occurs mainly near the branch bases, creating clusters of twigs called witches brooms. Salt can also get into soil in several ways, including being sprayed by traffic, plowed off the road, or by draining with melted snow and ice onto soil surfaces. Salt in the soil can kill roots directly or can prevent them from taking up water, causing drought injury. Often, injury cased by salt exposure does not show up for ...
Rabbit anti-rat cytochrome P450 enzyme CYP2D1 (polyclonal antibody). O-demethylation of xenobiotics, CYP2D6 is involved in the metabolism and elimination of approximately 25% of clinically used drugs in humans1. Primarily expressed in the liver and areas of the CNS. CYP2D1 and CYP2D4 have a similar function in rats2.. CODE NUMBER: RK05.. QUANTITY: 0.1 mL.. SPECIFICITY: Reacts with rat cytochrome P450 enzyme CYP2D1 in hepatic microsomal fraction.. IMMUNOGEN: Synthetic peptide.. SUGGESTED APPLICATIONS: Western blot, immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user.. SPECIES REACTIVITY: Rat.. FORMAT: Rabbit antiserum.. PRESENTATION: Liquid. No preservatives.. STORAGE/HANDLING: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.. For research use only; not for use in diagnostic procedures. Not for human or animal consumption.. CYP2D1 is also known as CYPIID1, Cyp2d-1, Cyp2d9 and ...
Rabbit anti-human cytochrome P450 enzyme CYP3A4 (polyclonal antibody). Heme-thiolate monooxygenase, involved in metabolism of several steroids, fatty acids, and xenobiotics. CYP3A4 & 5 are the two most predominant proteins within the CYP3A subfamily in human adults, which are collectively involved with the biotransformation of 50% of oxidatively metabolised drugs1. CODE NUMBER: R2X.. QUANTITY: 0.1 mL.. SPECIFICITY: Reacts with human cytochrome P450 enzyme CYP3A4 in hepatic microsomal. fraction from adult donors. Also binds to the fetal form: CYP3A7. No cross-reactivity with CYP3A5.. IMMUNOGEN: Synthetic peptide.. SUGGESTED APPLICATIONS: Western blot, immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user.. SPECIES REACTIVITY: Human.. FORMAT: Rabbit antiserum.. PRESENTATION: Liquid. No preservatives.. STORAGE/HANDLING: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.. For research ...
AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 7 days; Frozen (≤ -20°C): 3 months if frozen within 24 hours of collection (avoid repeated freeze/ thaw cycles) ...
Study evaluates the effects of freeze-thaw cycles on moisture migration, protein degradation, microstructure and quality of Pacific white shrimp.
The objective of this study was to evaluate and compare different sample treatments and storage conditions in order to determine optimal conditions for total and acylated canine ghrelin measurement using ELISA assays. Under the conditions assessed in this study, total ghrelin concentration was stable when stored at room temperature or under cooled conditions (4°C) for approximately six hours regardless of the type of anticoagulant used. When samples were stored at under −20°C, three freeze/thaw cycles during storage did not alter total ghrelin concentration if serum or plasma treated with EDTA or with EDTA-aprotinin is used. When samples were stored at under −80°C, no significant differences were observed after three freeze/thaw cycles in total ghrelin concentrations in serum or plasma treated with EDTA, EDTA-aprotinin or heparin. While acylated ghrelin showed to be very instable, its analysis should be performed within an hour of sample collection or stored at −80°C preferably in ...
TY - JOUR. T1 - Free polyunsaturated fatty acids cause taste deterioration of salmon during frozen storage. AU - Refsgaard, Hanne. AU - Brockhoff, P.M.B.. AU - Jensen, Benny. PY - 2000. Y1 - 2000. N2 - Increased intensity of train oil taste, bitterness, and metal taste are the most pronounced sensory changes during frozen storage of salmon (Refsgaard, H. H. F.; Brockhoff, P. B.; Jensen, B. Sensory and Chemical Changes in Farmed Atlantic Salmon (Salmo salar) during Frozen Storage. J. Agric. Food Chem. 1998a, 46, 3473-3479). Addition of each of the unsaturated fatty acids: palmitoleic acid (16:1, n - 7), linoleic acid (C18:2, it - 6), eicosapentaenoic acid (EPA; C20:5, it - 3) and docosahexaenoic acid (DHA; C22:6, n. - 3) to fresh minced salmon changed the sensory perception and increased the intensity of train oil taste, bitterness, and metal taste. The added level of each fatty acid (similar to 1 mg/g salmon meat) was equivalent to the concentration of the fatty acids determined in salmon stored ...
Fingerprint Dive into the research topics of Time of flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project. Together they form a unique fingerprint. ...
The winter hibernation in northern North America is very important because of how long and extreme it can be. To up the challenge the wood frog hibernates in the leaf litter and consequently freezes solid during winter. By solid I mean properly solid. The frogs can survive multiple freeze/thaw events as long as less than 65% of their water remains unfrozen. Freeze/thaw stops my experiments on bacteria from working but apparently these frogs are just fine. To survive the big freeze the frogs accumulate urea in the tissues and build enormous glycogen stores which breaks into glucose upon ice formation in the tissues. The soluble urea and glucose prevent ice formation and save the frogs. This phenomenal life cycle enables easier and early access to the natal ponds where eggs are laid and tadpoles develop and must leave before the thawing dries up the pools. So the question was posed, is a wood frog alive during winter hibernation? Technically its dead, no heartbeat, no metabolism and yet it ...
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles ...
TY - JOUR. T1 - Urinary p75ECD: A prognostic, disease progression, and pharmacodynamic biomarker in ALS. AU - Shepheard, Stephanie. AU - Wuu, Joanne. AU - Cardoso, Michell. AU - Wiklendt, Lukasz. AU - Dinning, Philip. AU - Chataway, Timothy. AU - Schultz, David. AU - Benatar, Michael. AU - Rogers, Mary-Louise. PY - 2017. Y1 - 2017. N2 - Objective: To evaluate urinary neurotrophin receptor p75 extracellular domain (p75 ECD) levels as disease progression and prognostic biomarkers in amyotrophic lateral sclerosis (ALS). Methods: The population in this study comprised 45 healthy controls and 54 people with ALS, 31 of whom were sampled longitudinally. Urinary p75 ECD was measured using an enzyme-linked immunoassay and validation included intra-assay and inter-assay coefficients of variation, effect of circadian rhythm, and stability over time at room temperature, 4°C, and repeated freeze-thaw cycles. Longitudinal changes in urinary p75 ECD were examined by mixed model analysis, and the prognostic ...
Structural changes underlying exocytosis evoked by the application of endotoxin to Limulus amebocytes were studied at the level of detail afforded by freeze-fracture and freeze-substitution techniques combined with the time resolution of direct rapid-freezing. The results with amebocytes prepared in this manner differed from those with other secretory cells prepared by conventional means. Exocytosis begins within seconds of endotoxin treatment when the plasmalemma invaginates to form pedestallike appositions with peripheral secretory granules. The juxtaposed membranes at these pedestal appositions form several punctate pentalaminar contacts, but examination of freeze-fractured pedestals failed to reveal any corresponding changes in the intramembrane particle distribution. Small secretory granule openings or pores, which are very infrequent, appear within the first 5 s after endotoxin treatment. These pores rapidly widen and this widening is immediately followed by the sequential dissolution of the
Many hibernating animals thermoregulate during torpor and defend their body temperature (Tb) near 0°C by an increase in metabolic rate. Above a critical temperature (Tcrit), animals usually thermoconform. We investigated the physiological responses above and below Tcrit for a small tree-dwelling bat (Chalinolobus gouldii, ∼14 g) that is often exposed to sub-zero temperatures during winter. Through simultaneous measurement of heart rate (fH) and oxygen consumption (V̇O2), we show that the relationship between oxygen transport and cardiac function is substantially altered in thermoregulating torpid bats between 1 and −2°C, compared with thermoconforming torpid bats at mild ambient temperatures (Ta 5-20°C). Tcrit for this species was at a Ta of 0.7±0.4°C, with a corresponding Tb of 1.8±1.2°C. Below Tcrit, animals began to thermoregulate, as indicated by a considerable but disproportionate increase in both fH and V̇O2. The maximum increase in fH was only 4-fold greater than the average ...
Jim Hartley ,jhartley at access2.digex.net, writes: ,for differing suspensions (i.e. plasmids). , ^^^^^^^^ .... : Do you mean that repeated freezing and thawing can do damage to : my plasmid DNA preps? , A few years back a guy here took a plasmid and cycled it between 37 C and , dry ice, my memory is 30 times, and took out samples during the process. , By gel analysis the degree of supercoiling was entirely unchanged by all , this freezing and thawing. I know there is an ancient bias that freezing , is bad for plasmids, but for DNA less than lambda size I have never seen , any data demonstrating this. I fear microbial growth and slow enzymatic , degradation much more than freeze/thaw cycles, so all my DNA is tucked away , in a frost free -20 freezer. , , Jim Hartley , Life Technologies, Inc. Thanks for the response Jim. I did know about the old BRL study, and I was trying to see if Ted meant all samples in the freezer, including plasmid DNA. I did e-mail him about the study done by BRL that ...
Labnet R8300 Polypropylene freezer storage boxes withstand autoclaving and freezing to -80ºC. The 3-point hinged lid maintains its integrity during repeated use. A positive catch keeps the lid securely fastened. Alpha-numeric indexing is printed inside the boxes for easy sample ID, and the clear lid allows visibility even when the it is closed. Boxes are available in a variety of colors for coding samples and a solid black box is available for light sensitive samples. The freezer boxes measure 142 x 142 x 55 mm and hold one hundred 1.5/2.0 mL tubes, cryovials or chromatography vials. The clear plastic work station racks come in two versions, to hold 40 x 0.5 mL microtubes or 40 × 1.5/2.0 mL microtubes. Robust construction makes sturdy racks for every day usage. They are autoclavable and the open bottom allows their use in ice baths.
Seasonal cold hardiness of red raspberry (Rubus idaeus L.) canes was measured by freeze-induced electrolyte leakage test and visual rating of injury. Leakage data were transformed to percentage-adjusted injury values and related to lethal temperature by graphical interpolation and by the midpoint (T50) and inflection point (Tmax) estimates derived from three sigmoid (the logistic, Richards, and Gompertz) functions. Tmax estimates produced by Richards and Gompertz functions were corrected further using two different procedures. The 10 leakage-based hardiness indices, thus derived, were compared to lethal-temperature estimates based on visual rating. Graphical interpolation and Tmax of the logistic or T50 of the Gompertz function yielded lethal-temperature estimates closest to those obtained visually. Also, Tmax values of the Gompertz function were well correlated with visual hardiness indices. The Richards function yielded hardiness estimates deviating largely from visual rating. In addition, the ...
A scientist lowers biological samples into a liquid nitrogen storage tank at the Cancer Research UK Cambridge Institute on December 9, 2014 in Cambridge, England. Healthy and cancerous biological... Get premium, high resolution news photos at Getty Images
TY - JOUR. T1 - Crumb rubber used in concrete to provide freeze-thaw protection (optimal particle size). AU - Richardson, Alan. AU - Coventry, Kathryn. AU - Edmondson, Vikki. AU - Dias, Eli. PY - 2016/1/20. Y1 - 2016/1/20. N2 - This research has examined the optimum particle size of crumb rubber, used as an additive to concrete that would provide maximum freeze-thaw protection whilst minimising the compressive strength loss. The crumb rubber as used in the paper was divided into five batches, with increasing particle size, graded in increments of 0.5mm, from 2.5mm. The primary properties of the concrete investigated were; air content, freeze-thaw durability and compressive strength. These were tested using standard test methods. The range of tests used were conclusive in that the ,0.5mm crumb rubber particle size, provided the greater degree of air entrainment. The freeze-thaw cycle results suggested that crumb rubber provided freeze-thaw protection, as the plain concrete deteriorated compared ...
Maneuver your liquid nitrogen storage dewar safely and easily with a roller base from by Brymill Cryogenic Systems, a provider of cryogenic equipment.
In this study, we developed and validated a sample preparation protocol for unbiased amplification and high-throughput metagenomic sequencing of viruses in routine diagnostic use. In an unbiased metagenomic approach, prior knowledge of the virus sequence is not required. In principle, it can therefore detect any virus. However, sample processing needs to be optimized for recovery and amplification of viral genomes that might be present only in very small amounts in clinical samples. We optimized such a protocol using plasma samples spiked with different classes of viruses.. First, we tested filtration, extraction, and nuclease digestion procedures in order to find optimal conditions for virus enrichment and reduction of unwanted, non-viral reads. Filtration proved to be indispensable, as the number of virus reads after sequencing significantly increased. Additionally, sample pre-processing including a freeze thaw cycle showed the best enrichment (Fig. 1), and for best integration into the daily ...
Cyclamen can grow in zones 5/6 as long as a few rules are observed. Hederifolium puts out roots from the top and sides of the tuber, unlike Cyclamen coum and the like which have roots on the bottom surface only, so they need to be planted with the tops of the tubers a couple inches deep (place soil up to the top and then cover the top with a couple inches of coarse grit rather than garden soil). The best locations are on a north facing slope under a tree, or on the north side of the house that doesnt get any direct sun in the winter. Sunlight makes the normal freeze/thaw cycles even worse. Note: if you see ants on these plants dont worry, this is a good thing because they disperse the seed ...
article{d127567d-2071-4748-b996-09807a0c4a66, abstract = {At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant negative effect on the polyunsaturated PLFA, linoleic acid (18:2 omega 6,9), which has previously been reported to correlate positively (R-2 = 0.92) with freeze tolerance in D. octaedra. This supports our ...
BISAC: SCI013000. In petroleum engineering, liquid nitrogen can be used to aid in fracturing the formation rock because of its extremely cryogenic temperature. With the development of new jet technology in recent years, liquid nitrogen is considered as a promising jet medium to exploit oil and gas. The first chapter of this book provides an impact pressure and parametric sensitivity analysis of the numerical simulation of liquid nitrogen.. In the second chapter, the uses of liquid nitrogen in a completely different sphere are explored, particularly its use for the storage of human cells and tissues for transplantation. Finally, liquid nitrogen is one of the cryogenic fluids most used for cryopreservation of animal germoplasm; low temperature preservation of oocytes, sperm and embryos is a fundamental cornerstone of assisted reproductive technologies. The last chapter examines the mathematical prediction of cooling rates during cryopreservation of reproductive cells in liquid nitrogen ...
Transgenic Rat or Mouse Spinal Cord (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells - Science Exchange Lets You Compare Quotes From Leading Service Providers.
Transgenic Rat or Mouse Sciatic Nerve (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells - Science Exchange Lets You Compare Quotes From Leading Service Providers.
Oogenesis was examined in nine species of Antarctic fish to verify the existence of morphological peculiarities. The analyses were carried out on specimens belonging to three different families of Notothenioids (Nototheniidae, Channichthyidae and Bathydraconidae), all captured in the Ross Sea, in front of the Italian Station of Terra Nova Bay. Following dissection, the ovaries were processed and examined at the light and electron microscopes to determine the oocyte gross and fine morphology. The attention, in particular, was focused on the presence of cytoplasmic round bodies and on the organization of the cortical alveoli and the vitelline envelope. Results reveal significant specie-specific differences that could be partly correlated to the phylogenetic radiation but not to the peculiar environmental conditions being essentially comparable to those observed among temperate species.. ...
STONY BROOK, NY, August 7, 2020 - Ice crystal formation plays a crucial role in precipitation formation and alters the radiative properties of clouds, thereby affecting Earths climate system. In recent years there has been tremendous progress in understanding how liquid-phase droplets are formed (nucleation), yet the nucleation of ice crystals has remained elusive. Thats why Daniel Knopf, PhD, Professor in the School of Marine and Atmospheric Sciences (SoMAS) at Stony Brook University, is studying the nucleation of ice crystals with the goal to improve current cloud and climate modeling approaches. The research is supported by a three-year $710,000 grant from the Department of Energy that starts September 15, 2020.. Ice formation is considered one of the remaining grand challenges in the atmospheric sciences, says Professor Knopf. The objective of the project is to gain a predictive understanding of the chain that leads from aerosols to ice-nucleating particles to ice crystal number ...
Need a liquid nitrogen dewar to rent? Do you want to make liquid nitrogen ice cream at home? Do you like experimenting with liquid nitrogen? Do you need a liquid nitrogen dewar or cryogun for your doctors office? If so, you came to the right place. We sell and rent liquid nitrogen dewars for cooking, experimenting and also medical use ...
liquid nitrogen Manufacturers & liquid nitrogen Suppliers Directory - choose liquid nitrogen from 2196 Jiangsu liquid nitrogen Super Suppliers of China.
liquid nitrogen Manufacturers & liquid nitrogen Suppliers Directory - choose liquid nitrogen from 2148 Sichuan liquid nitrogen Super Suppliers of China.
Human CALY Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. This is a Recombinant Protein Lysate produced in HEK293T cells. This protein lysate can be reconsituted using SDS Sample Buffer. Once reconsituted, aliquot and store any remaining material at -20C to avoid repeated freeze-thaw
Hnasko, R.M., Lin, A.V., Stanker, L.H., Bala, K., McGarvey, J.A. 2016. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria. In: Micic, M., editor. Sample Preparation Techniques for Soil, Plant, and Animal Samples. New York, NY: Humana Press. p. 389-399 ...
Introduction. Level 1 Pharmacy Physicochemical Principles of Pharmacy 315PMY105 Name: Catherine Magee Student number: 13324063 Practical: 3 - Freezing Point Depression Aims � Demonstration of the effect of solutes used in aqueous formulations on the freezing point of water. � Application of the knowledge of the depression of freezing point to the determination of the molecular weight of an unknown sample. Learning Objectives � Development of the laboratory skills related to weighing samples, making solutions and measuring freezing point depression values � To increase the understanding of the colligative properties of electrolytes and non-electrolytes � Development of computer skills in excel. Introduction Depression of freezing point is one of the colligative properties easiest to measure (others are vapour pressure, boiling point elevation, osmotic pressure). Colligative properties depend on the number of particles present in a solution. The equation for depression of freezing point ...
a The β-galactosidase activity assay was performed as described in the MATCHMAKER two-hybrid system (20). Briefly, cells from 1.5-ml samples of exponential culture were collected by centrifugation and resuspended in 300 μl of Z-buffer (20). A 100-μl aliquot of the resuspended cells was lysed by quick freeze-thaw (treatment with liquid nitrogen followed by thawing at 37°C). To measure the β-galactosidase activity in the cell lysate, a 0.7-ml sample of the Z-buffer-β-mercaptoethanol solution was added to each tube followed by 0.16 ml of Z-buffer-ONPG (4 mg of ONPG per 1 ml of Z-buffer). The time of ONPG addition was recorded, and the tubes were incubated at 30°C with shaking. When yellow color was visible, 400 μl of 1 M NaCO3 was added to each tube to terminate the reaction, and the time was recorded. The tubes were then centrifuged for 10 min at 10,000 × g to remove cellular debris, and the optical density at 420 nm was recorded. β-Galactosidase units were defined as the amount of ...
Liquid nitrogen expands 700 times in volume when changing from liquid to gas. By injecting a small amount of liquid nitrogen just before the container is sealed, the expanding nitrogen gas will displace the air in the container. This creates an oxygen free (inert) environment to extend product life and can give a slight pressurization to thin wall packages if needed to improve container durability.. A continuous stream liquid nitrogen injection can be wasteful at slower line speeds and will not detect missing containers or the complete lack of containers on the packaging line. Technifabs Techinjector, using a sensor on the packaging line, detects each container and precisely injects a measured dose of liquid nitrogen at the proper time. The Programmable Logic Controller (PLC) unit automatically adjusts for line speed changes and does not dose missing containers. The unit can also be set up to run in a continuous mode and then shut off when containers stop coming down the production line.. The ...
Egg freezing has become more accepted over the past few years as a method of fertility preservation for a number of reasons. Before 2013, anyone who froze their eggs had to do so under an experimental protocol. But after studying egg freezing in depth, the American Society of Reproductive Medicine issued a statement that there is enough scientific evidence showing that egg freezing works and that it should longer be considered experimental.. Two important factors influenced this position: First, new egg freezing technology was introduced called vitrification, a fast-freeze method that almost instantly transforms the eggs into a glass-like frozen state. Vitrification reduces the likelihood that the fluid in the egg will form ice crystals, which could damage it. Multiple studies of egg freezing done via vitrification demonstrated that it was much more successful than the older method called slow freeze.. Second, a number of studies were done using donor eggs where it was reliably determined that ...
The quality of clinical biobank samples is crucial to their value for life sciences research. A number of factors related to the collection and storage of samples may affect the biomolecular composition. We have studied the effect of long-time freezer storage, chronological age at sampling, season and month of the year and on the abundance levels of 108 proteins in 380 plasma samples collected from 106 Swedish women. Storage time affected 18 proteins and explained 4.8-34.9% of the observed variance. Chronological age at sample collection after adjustment for storage-time affected 70 proteins and explained 1.1-33.5% of the variance. Seasonal variation had an effect on 15 proteins and month (number of sun hours) affected 36 proteins and explained up to 4.5% of the variance after adjustment for storage-time and age. The results show that freezer storage time and collection date (month and season) exerted similar effect sizes as age on the protein abundance levels. This implies that information on ...
Custom BioGenic Systems offers only the highest quality in dry storage Isothermal Liquid Nitrogen Freezers. These models offer a wide lid opening to allow access to all racks, whilst providing an extensive variety of inventory solutions. We offer multi-tired straw storage systems, split samples, chest racks and cryo bag storage options. The vapour circulation provides better visability in the storage area.. These dry storage units offer additional user safety by eliminating liquid nitrogen contact. CBS dry storage units also minimize sample cross-contamination by eliminating liquid nitrogen submersion.. Mandel offers a complete selection of freezer sizes from 30L to 140L liquid nitrogen capacity options.. ...
Description: Standard 3 \ Stainless Steel Box w/Drain Holes Packaging: Bagged Size: 1g Qty: Dispenser with Slatwell Bracket 3 Unit: Pckg. of 12 Product: X-Gluc Quantity: 1 Tubes: 1.5/2.0ml L x W: 140 x 7mm Quantity/Unit: 10 Cell Size (mm/in
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Fiber- och polymerteknologi. KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Centra, Wallenberg Wood Science Center.ORCID-id: 0000-0002-5444-7276 ...
We are pleased to introduce a new application for one of our neural progenitor markers. In this study our Chicken Nestin Antibody is used to stain these progenitors in dissected mouse brains: Gleave JA, Lerch JP, Henkelman RM, Nieman BJ (2013) A Method for 3D Immunostaining and Optical Imaging of the Mouse Brain Demonstrated in Neural Progenitor Cells. PLoS ONE 8(8): e72039. doi:10.1371/journal.pone.0072039. 3D antibody staining of adult mouse brains: 1% PFA perfused adult mouse brains were removed from the skull and then divided by removing the cerebellum and separating the hemispheres if desired. The samples were immediately dehydrated in a gradient of methanol solutions to 100% methanol over the course of one day. Immediate dehydration is important to preserve the cellular morphology of the lightly-fixed brain. The samples then underwent freeze/thaw (one hour at -80°C/one hour at room temperature) four times. The samples were rehydrated in a gradient of methanol solutions to PBS over the ...
Because the liquid-to-gas expansion ratio of nitrogen is 1:694 at 20 °C (68 °F), a tremendous amount of force can be generated if liquid nitrogen is rapidly vaporized in an enclosed space. In an incident on January 12, 2006 at Texas A&M University, the pressure-relief devices of a tank of liquid nitrogen were malfunctioning and later sealed. As a result of the subsequent pressure buildup, the tank failed catastrophically. The force of the explosion was sufficient to propel the tank through the ceiling immediately above it, shatter a reinforced concrete beam immediately below it, and blow the walls of the laboratory 0.1-0.2 m off their foundations.[17]. Because of its extremely low temperature, careless handling of liquid nitrogen and any objects cooled by it may result in cold burns. In that case, special gloves should be used while handling. However, a small splash or even pouring down skin will not burn immediately because of the Leidenfrost effect, the evaporating gas thermally insulates to ...
Nov 24, 2019 Liquid Nitrogen Facts . Liquid nitrogen is the liquefied form of the element nitrogen thats produced commercially by the fractional distillation of liquid air. Like nitrogen gas, it consists of two nitrogen atoms sharing covalent bonds (N 2).; Sometimes liquid nitrogen is denoted as LN 2, LN, or LIN.; A United Nations Number (UN or UNID) is a four-digit code used to identify flammable and ... Read More ...
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... freezing; drying; marketing; storing; packing for shipment; or distributing". The opinion in the case said that 43 of the 50 U. ... freezing, drying, marketing, storing, packing for shipment or distribution" of certain goods were activities exempted from the ...
During the freeze, the frog's breathing, blood flow, and heartbeat cease. Freezing is made possible by specialized proteins and ... Articles concern any aspect of low-temperature biology and medicine (e.g. freezing, freeze-drying, hibernation, cold tolerance ... freezing down' a sample to better preserve it for eventual thawing, before it is deep frozen, or cryopreserved, in liquid ... the frozen eggs are thawed and sperm is placed with the eggs and together they are placed back into the uterus or a frozen ...
Freezing". The Times. London. Retrieved 22 February 2008. CS1 maint: discouraged parameter (link) Wollaston, Sam (23 February ...
"Accelerating Turn". Freezing. Season 1. Episode 3. Doi, Hitoshi (May 11, 2017). "Search results for "Ohkubo Rumi" in ALL ...
CS1 maint: discouraged parameter (link) Knox, David (30 July 2008). "Airdate: Freezing". tvtonight.com.au. Retrieved 30 July ...
"Freezing Notes." SHOAC. "Auxiliaire Facilities." SHOAC. "Restaurant." SHOAC. "Cafe & Gift Store." SHOAC. "Antique Music Box ...
... prisoners were forced to sit in tanks of freezing water for up to three hours. After subjects were frozen, they then underwent ... The freezing/hypothermia experiments were conducted for the Nazi high command to simulate the conditions the armies suffered on ... The results of the Dachau freezing experiments have been used in some late 20th century research into the treatment of ... I'm trying to make something constructive out of it." Dr John Hayward, justifying citing the Dachau freezing experiments in his ...
Winter Storm in Texas , No Electricity, Heat X 3 days , Freezing Temperatures , Limited Water Supply, retrieved 2021-02-19 11- ...
"Q&A:Frozen sperm". BBC News. 25 May 2004. Adam, David (2004-05-27). "How long can frozen sperm be stored?". the Guardian. ... Frozen vials of donor sperm may be shipped by the sperm bank to a recipient's home for self-insemination, or they may be ... Before freezing, sperm may be prepared (washed or left unwashed) so that it can be used for intracervical insemination (ICI), ... It has been proposed that there should be an upper limit on how long frozen sperm can be stored; however, a baby has been ...
Egg freezing. A woman can freeze her eggs preserve her fertility. By using egg freezing while in the peak reproductive years, a ... woman's oocytes are cryogenically frozen and ready for her use later in life, reducing her chances of female infertility. There ...
Slow freezing. This method relies on the mechanism of freeze dehydration to pull water out of the cells and thus prevent ice ... By freezing at an ultra-fast rate and using osmotic dehydration, the water that is still present in the cell is unable to form ... The following years, new methods to cryopreserve were developed, such as direct immersion, slow freezing and vitrification, as ... started in 1965 when Hirai was studying the biology activities that happened when biological samples were frozen. Three years ...
"Freezing breath!"). Phantom Girl compliments his skills (and thinks to herself that he's cute). In the first-season finale, Jo ...
... freezing chamber; warehouse for supplies; incinerator and compactor for residues; installations for fuel pump, heat generation ...
The entire swarm was frozen by Aqua Ryukendo's Freezing Slash. Elemoon (エレムーン, Eremūn, 11): A Jack Moon clone powered by the ... Macode was frozen/destroyed by Blizzard Ryukendo's Exploding Freezing Slash. Magical Satellite Grenstar (魔法衛星グレンスター, Mahō Eisei ... His finishing move is Freezing Slash (氷結斬り, Hyōketsu Giri). When God Ryukendo uses the Blizzard Key, the upgraded form of his ... Akebono City also snows from the Freezing Slash made from Aqua Ryukendo. The Demon Beasts (魔獣, Majū) are usually created by Dr ...
Peter Vidani (2 June 2013). "Freezing Tumblr". Papyh.tumblr.com. Retrieved 15 August 2013. Reinthaler, Joan (10 September 2010 ...
It was established on 7 January 1912 to store frozen bait, and in the 1930s moved into freezing fish. In 1970 Gunnvör hf. and ... The commission encouraged fishermen to start quick-freezing their catch and began to research the market for frozen fish. The ... Ísafjörður Freezing Plant Company) was an Icelandic company that produced frozen fish for export. ... The main purpose was to freeze and store herring and other bait, as well as to store food for people of the town and other ...
"Fremantle Freezing Works". Toodyay Herald. Western Australia. 28 May 1921. p. 5. Retrieved 24 January 2020 - via Trove. "Lamb ... In 1921 the Fremantle Freezing Works began operation as one of the three State Government regulated abattoirs under the 1909 ... CS1 maint: discouraged parameter (link) "FREMANTLE FREEZING WORKS". Sunday Times (Perth). Western Australia. 24 August 1919. p ...
"Freezing physics" (PDF). p. 260. See: Zeeman, P. (1896). "Over de invloed eener magnetisatie op den aard van het door een stof ...
... freezing-effect'; conceiving 3D camera movements in a photorealistic environment reproduced by utilizing still photographic ...
Freezing may also occur when neither fight or flight is an available option. If there is no decision to make, freezing is not a ... Freezing is a primary instinctive survival response along with fight and flight Freezing is an automatic, involuntary response ... "Freezing with Fear". Psychology Today. Retrieved 2020-07-19. "Why do we freeze when we're scared?". Popular Science. Retrieved ... or Freeze: How We Respond to Threats". Healthline. 2020-02-21. Retrieved 2020-07-19. " ...
... powerful freezing breath; heat vision; and flight. In the DC Bombshells continuity, Faora led a coup on Krypton during its ...
ISBN 978-1-58008-450-5. P. Hansen; J. Aagaard (2008). "Freezing of Shellfish". In Rudolf Kreuzer (ed.). Freezing and ... It may fetch very high prices and may be sold fresh, frozen, canned or powdered. Both the claws and the abdomen of H. gammarus ...
Both fresh tissue and fixed tissue can be frozen. Moreover, it is also a technique used for freezing sections of either fresh ... "Tissue Freezing Methods for Cryostat Sectioning" (PDF). Knox, R. B. (1970). "Freeze-sectioning of plant tissues". Stain ... "Freezing tissues for histology" (PDF). J. Byrwa-Neff, Kimberly; Cunningham, Miles (2012-07-12). "Freezing Biological Samples". ... During the freezing procedure, the water in tissues is easy to form ice crystals, which often affects the antigen localization ...
It was freezing. One of the coldest nights of the year. It took many hours for me and Bruno Tilley, the art director, to light ...
... powerful freezing breath; and flight. Being female, her power levels are more akin to Supergirl and Wonder Woman. Similar to ...
"Far Cry 2 freezes on Xbox 360". Product Reviews. January 7, 2009. Archived from the original on January 25, 2009. Retrieved ... "FarCry 2 freezing issues on Xbox 360: Magical number-62%". Console Community. January 7, 2009. Archived from the original on ... "Save game freezing? Buddies attacking you? Stuck at xx%?". Far Cry 2 Technical Community Help (Forum). November 4, 2008. ... freezes, and critical mission characters disappearing from the game. In the PC version of the game, several users were able to ...
"Freezing in Hell." Richard Chamberlain, Actor and Beyond, 1993. Retrieved: September 26, 2014. Gager, Scott J. "Review: Death ...
The Wellington Meat Export Co freezing works had a siding from 2 April 1917 and had three sidings when it opened in 1918. The ... "Old Kakariki Freezing Works MANAWATU TIMES". paperspast.natlib.govt.nz. 27 September 1940. Retrieved 6 February 2021. "Potato ... "FREEZING MERGER COMPLETE. HOROWHENUA CHRONICLE". paperspast.natlib.govt.nz. 21 May 1927. Retrieved 6 February 2021. "CLOSED ... 1940 photo of freezing works 1975 aerial photo of station and factory 2013 and 2014 videos of freight trains crossing the ...
... powerful freezing breath; heat vision; and flight. In his Post-Infinite Crisis Kryptonian iteration, Dev-Em is never allowed to ...
... powerful freezing breath; heat vision; and flight. His powers are generally depicted as being on par with General Zod. Jax-Ur ...
Freezing is a phase transition where a liquid turns into a solid when its temperature is lowered below its freezing point. In ... Main article: Frozen food. Freezing is a common method of food preservation that slows both food decay and the growth of micro- ... But this can be understood since heat must be continually removed from the freezing liquid or the freezing process will stop. ... the freezing is greatly slowed and the temperature will not drop anymore once the freezing starts but will continue dropping ...
Flash freezing is used in the food industry to quickly freeze perishable food items (see frozen food). In this case, food items ... How water freezes[edit]. There are phenomena like supercooling, in which the water is cooled below its freezing point, but the ... In physics and chemistry, flash freezing is the process whereby objects are frozen in just a few hours[1] by subjecting them to ... Flash freezing techniques are used to freeze biological samples quickly so that large ice crystals cannot form and damage the ...
Freezing behavior or the freeze response is a reaction to specific stimuli, most commonly observed in prey animals. When a prey ... Not only did these investigations show that the hippocampus is involved with freezing behavior, but avoidance and freezing ... That suggests that neurotransmitters, in general, play a role in freezing behavior. Several investigations show that freezing ... or freeze." In addition, freezing is observed to occur before or after a fight or flight response. Studies suggest that ...
The temperature plateaus at -10 degrees celsius but the alcohol does not freeze. How can you explain this result? ... A student uses a home freezer in an experiment to find the freezing point of rubbing alcohol. ... Freezing Point? A student uses a home freezer in an experiment to find the freezing point of rubbing alcohol. The temperature ... to -10 C, and the freezing point of the alcohol is lower than that. -10 C is good for keeping food, any lower will be a waste ...
Why the Midwests deep freeze may be a consequence of climate change More than a quarter of the U.S. population is expected to ... Much of the American Midwest is experiencing a deep freeze that will bring with it the lowest temperatures seen in a generation ... Trenton and Baltimore all broke temperature records during a burst of arctic air that wreaked havoc by freezing pipes. Also, ...
Methods of measuring freezing time are described in Advisory Note 94.. Fig. 1 FREEZING TIME OF BLOCKS 10cm THICK IN DIFFERENT ... Handling before freezing. Size grading. Freezing time. Unloading the freezer. Introduction. This note gives advice on the ... Freezing time. The freezing time of a block of small fish in a thin bag with added water is about the same as an unwrapped ... General advice on freezing and cold storage of fish is given in Advisory Notes 27 and 28, and advice on the freezing at sea of ...
If you leave water out for your neighborhood cats, take a look at these tips for preventing freezing water in the cold months. ...
... but developing countries can also develop their own frozen food industries. Introduction of adequate freezing technology, based ... Freezing is the most widely used method of food preservation permitting retention of quality of the products during long ... Currently, the frozen food market is one the largest sectors in the food industry. Industrialized countries dominate the trade ... Compared to other conventional methods used in the storage of fruits and vegetables, freezing is the most satisfactory method ...
... Area: Chemistry Posted By: Chris Larson, Post-doc/Fellow Laboratory of Genetics. Date: Tue Feb 11 22:01:15 ... I cant think of any reason why contact with warm air would make a beer that had been chilled in the freezer freeze, but I can ... I read the question and answer to Freezing Beer? and would like to offer an alternate explanation. I forget which of the gas ... Then take out half very carefully and half with a lot of vibration and motion and see if the latter will freeze without you ...
To freeze columns. * In the PowerPivot window, select the table that contains the columns that you want to freeze. ... On the Home tab, in the View group, click Freeze.. A frozen column moves to the front of the table. Additional columns that you ... freeze are positioned next to the first column that you froze. If you later unfreeze the column, it will not be moved back to ... you can lock specific columns in one area by freezing them. ...
Bitterly cold temperatures and blizzards are making life painful across Russia, Ukraine, and Belarus. Health authorities in Kyiv say at least 37 people have died in Ukraine from causes attributed to cold weather. In Russias Far East, temperatures have plummeted to minus 50 degrees Celsius. (17 PHOTOS)
Freezing most often affects walking,but it also can affect speech,writing,and the persons ability to open and close his or her ... Freezing (sometimes called motor block) is a sudden,brief inability to start movement or to continue rhythmic,repeated ... Articles OnParkinsons Disease and Freezing. Parkinsons Disease and Freezing Parkinsons Disease and Freezing * Topic Overview ... Freezing may occur at an open doorway (most common), at a line on the floor, or in crowds. It may be more likely to occur if ...
Learn tips for freezing this vegetable from experts at HGTV Gardens. ... Freezing Brussels sprouts when theyre in season is worth the effort. ... You have two options for freezing: individual quick freeze or package freezing.. Quick-freeze Brussels sprouts individually on ... Freezing Brussels Sprouts Its worth freezing this cabbage cousin when you have homegrown or locally raised versions that boast ...
How to Freeze a Body If what Ferrell says is true, Ted Williams would join a rapidly growing group of Americans: the frozen. ... Of the 41 people frozen at the Cryonics Institute in Clinton Township, Mich., open since 1976, about half were frozen in the ... If Williams son wanted to harvest his fathers genes for the highest bidder, he could simply freeze a piece of his fathers ... Some reports Monday said Williams body had already been frozen, but the process of submerging a body in liquid nitrogen takes ...
5.0 out of 5 stars Excellent for cereal and freezing; strong airtight seal ...
FROZEN. Seagull Media Prods. in association with Phat Phish Motion Pictures. Credits: Director/writer/producer: Shivajee ... In fact, the story of "Frozen" is the story of Lasya as she grows up watching a subtle evolution around her, and Chomo, her ... Shanker Ramans lens freezes the grim, gray scenery, highlighting the cunning of the moneylenders and the opportunism of Romeo ... CHENNAI, India -- Shivaji Chandrabhushans first feature, "Frozen," conveys in no uncertain terms his love for photography and ...
Frozen shoulder means your shoulder is painful and stiff for months, sometimes years. It can be treated with shoulder exercises ... How long frozen shoulder lasts. Frozen shoulder can take at least 1.5 to 2 years to get better. Sometimes it can be up to 5 ... Its often not clear why people get a frozen shoulder.. Frozen shoulder happens when the tissue around your shoulder joint ... Frozen shoulder means your shoulder is painful and stiff for months, sometimes years. It can be treated with shoulder exercises ...
... USA Today NetworkMary Bowerman, USA TODAY Network Published 10:47 a.m. ET Feb. 19, 2015 , Updated ... Lake Erie almost frozen over. The Great Lakes ice cover continues to grow as winter weather slams the country. Lake Erie had 98 ... Lake Erie almost frozen over The Great Lakes ice cover continues to grow as winter weather slams the country. Lake Erie had 98 ... In 2014, 90% of the Great Lakes were frozen solid, the highest level since 1979, according to the Great Lakes Environmental ...
Italian researchers have achieved 13 human births using previously frozen eggs. Its encouraging for women who want to preserve ... Its part of the reason this study came from Italy, because you can freeze eggs but you cant freeze embryos under this law. ... While men have had the option of freezing their sperm for years, egg freezing was next to impossible less than 20 years ago. ... In vitro fertilization with fresh, not frozen, eggs has about a 30 percent birth success rate, while in this study the freezing ...
Raw fruits which are low in acid content need treating before freezing to prevent discoloration on thawing. ... Preserving fruit by deep freezing is easy and very convenient because fruit can be frozen raw and eaten raw all the year round ... Cooked fruit can also be frozen as a means of preserving. Caution on three points:. 1. Do not overcook fruit to be frozen. ... Preserving fruit by deep freezing is easy and very convenient because fruit can be frozen raw and eaten raw all the year round ...
In temperatures close to freezing and waves eight meters high, the Italian coast guard took the migrants on board their small ... EU Pressured to Increase Patrols as Migrants Freeze to Death Crossing Mediterranean ...
According to new research, tweaking the freezing technique enabled scientists to more than double egg survival. Eggs generally ... preserving some of their eggs ahead of time by freezing them can leave open the possibility of having children. But such ... deteriorate during the freezing process because they are not sufficiently dehydrated. Excess water forms ice crystals, which ... increasing the amount of time the eggs were exposed to chemical protectants and boosting the amount of sugar in the freezing ...
The freezing technique transforms desmoid tumors into a gelatinous mass that is slowly resorbed by the body, although care is ... Over several freezing and thawing cycles, tumor cells die off, and the tumor turns into a necrotic mass with the consistency of ... Cite this: Freezing Desmoid Tumors Halts Progression, May Cure? - Medscape - Oct 22, 2019. ... Cryoablation was performed over two 10-minute freezing cycles. The cycles were separated by a passive 10-minute thawing period ...
Freezing temperatures endanger Athens homeless. Posted Jan. 8 - Winter hits Greeces homeless population, which has grown up ...
Annie Dauer decided to undergo a radical new treatment to freeze part of her ovary in hopes of preserving fertility-with ... Embryo freezing costs about $4,000 more. With ovary freezing, on the other hand, "you have the source," Oktay told The Daily ... One of the biggest differences between egg freezing and ovary freezing, preparation time, was the crux of Annies case. She ... With egg freezing, once a patient has finished their hormone treatments, a doctor will retrieve the eggs with a needle inserted ...
This article was originally published with the title "To Keep Cellars from Freezing" ... in which on several occasions vegetables have frozen, although the cellar was fortified against fjost by a process known to ...
The supercooled liquid will freeze rapidly on the addition of a crystal of sodium thiosulfate, or on stirring which seeds the ... Supercooling - the energetics of freezing. Class practical. Students melt sodium thiosulfate. crystals, and these are then ... The supercooled liquid will freeze rapidly on the addition of a crystal of sodium thiosulfate, or on stirring which seeds the ... The supercooled liquid will freeze rapidly on the addition of a crystal of sodium thiosulfate, or on stirring which seeds the ...
For the tastiest results, use top-quality garden-fresh produce and follow these freezing guidelines. ... Freezing produce is an easy way to enjoy fresh vegetables from the garden or the farmers market well into winter. ... General Freezing Steps. *For freezing, select vegetables at their peak of maturity. Hold produce in the refrigerator if it can& ... Using Frozen Foods. Vegetables are best cooked from a frozen state without thawing them first. Thaw fruits in their containers ...
The freezing process preserves nutrients and provides a fresher flavor than canning or drying foods. ... Freezing is a simple, easy and convenient way to preserve vegetables. The process takes little time, but the cost of a freezer ... Use frozen vegetables within one year.. Preparing Frozen Vegetables. Most frozen vegetables can be cooked without thawing; corn ... Blanch, cool promptly, drain, pack, seal and freeze. Frozen onions are suitable only for cooking. Freezing green onions is not ...
  • A student uses a home freezer in an experiment to find the freezing point of rubbing alcohol. (yahoo.com)
  • Dry Ice can't freeze isopropyl alcohol, you can bet your freezer won't either. (yahoo.com)
  • The temperature of -10 is simply as cold as the freezer can produce and has nothing to do with the freezing point of alcohol. (yahoo.com)
  • for example on a large freezer trawler single hauls of up to 50 tonnes are possible with a large midwater trawl, whereas the shipboard freezing capacity is unlikely to be more than 2 t/hour. (fao.org)
  • One of the most common forms of quality degradation due to moisture migration in frozen foods is freezer burn, a condition defined as the glassy appearance in some frozen products produced by ice crystals evaporating on the surface area of a product (Kaess, 1961). (fao.org)
  • I can't think of any reason why contact with warm air would make a beer that had been chilled in the freezer freeze, but I can think of an alternative explanation. (madsci.org)
  • If that works, then very carefully open the tops of the ones that you very carefully took out of the freezer and wait to see if they freeze upon contact with warm air. (madsci.org)
  • For fastest freezing, place the sheet close to where cold air enters your freezer. (hgtv.com)
  • After sprouts are frozen, tuck them into freezer bags in bulk. (hgtv.com)
  • Place bags into the freezer as flatly as possible until sprouts are frozen. (hgtv.com)
  • If moisture evaporates, frozen food becomes dry, tough and might develop grayish spots called "freezer burn. (osu.edu)
  • Examples are "can or freeze" glass jars, plastic freezing containers, heavyweight aluminum foil, plastic-coated freezer paper and polyethylene wrap and bags. (osu.edu)
  • To retain quality, freeze vegetables quickly at the lowest possible freezer setting. (osu.edu)
  • When placing packaged food in freezer spread out packages to enable them to freeze quicker. (hubpages.com)
  • One of the best ways to save time and make sure that you always have good food available for a last minute meal is to cook large portions of freezer friendly foods when you have time to cook, and then freeze. (hubpages.com)
  • Learn how to freeze vegetables and see how easy it is to fill your freezer with garden-fresh goodness. (hgtv.com)
  • When hunters brought game animals home in winter, it was possible to freeze the catch by using the outdoor environment as the freezer. (encyclopedia.com)
  • The appliance and food industries noted the acceptance by consumers of both freezing preservation of foods and the small freezer sections in household refrigerators. (encyclopedia.com)
  • Freezing the fish extends its life and keeps your freezer stocked with the nutrient-dense protein. (ehow.com)
  • Freezing the raw shrimp in water helps protect it from freezer burn. (ehow.com)
  • The Cryostage freezing stage is a refrigerated design for use as a specimen freezer and object holder, primarily for use with the 8000 sledge microtome, however, it can be used with other sledge microtomes. (bio-medicine.org)
  • The Society for Reproductive Technology says 10,936 women froze their eggs in 2017 and the market is projected to grow 25% annually over the next two years, according to Fertility IQ. (cnbc.com)
  • For years, health experts considered egg freezing experimental, with success rates below those for either embryo freezing or using fresh "donor" eggs. (webmd.com)
  • Researchers in Italy - a country with some of the most restrictive rules on embryo research - have achieved 13 live human births using frozen eggs. (wired.com)
  • Researchers have created an embryo using an egg produced by ovarian tissue that had been frozen for six years and re-implanted. (wired.com)
  • Annie knew that the radiation could damage her eggs, and jeopardize her future fertility-but egg or embryo freezing required weeks of preparatory hormone shots. (thedailybeast.com)
  • For years, egg and embryo freezing have been heralded as the gold standard in fertility preservation technology, allowing women to delay childbirth until they're ready-instead of the moment sperm meets egg. (thedailybeast.com)
  • Embryo freezing requires similar hormonal treatments-the key difference is that the eggs are fertilized in-vitro before they're frozen, not after. (thedailybeast.com)
  • Embryo freezing costs about $4,000 more. (thedailybeast.com)
  • The Frozen Embryo Transfer, commonly referred to as FET procedure involves thawing and then transferring the embryo into a woman's uterus for further pregnancy . (selfgrowth.com)
  • This frozen embryo transfer procedure does not involve any anesthesia and is rather painless. (selfgrowth.com)
  • A frozen embryo from a previous IVF cycle is used in this process. (selfgrowth.com)
  • Due to some advanced freezing techniques , like advanced flash-freezing vitrification technologies, Frozen Embryo Transfer generally does not have a negative impact on their success rate and result in increased pregnancy and live birth rates, decreased miscarriages, and result in healthier babies. (selfgrowth.com)
  • Various factors that might affect the success rate of a frozen embryo transfer include uterine factors such as fibroids or a uterine septum, as well as the quality and genetic content of the embryos, immunologic, environmental, and infectious factors. (selfgrowth.com)
  • Dr Shivani Sachdev Gour will evaluate for all these factors before performing the frozen embryo transfer at SCI Healthcare and SCI IVF Hospital and Multi-specialty Center. (selfgrowth.com)
  • In the past decade, scientists created a flash-freezing method called vitrification that appears to overcome that challenge. (yahoo.com)
  • But thanks in large part to a new technology called vitrification, egg freezing is ready for prime time. (webmd.com)
  • Vitrification takes all the water-like substances in the egg and transitions them without the cracking that can occur during freezing or thawing," Woodruff says. (webmd.com)
  • Because vitrification is a non-equilibrium process, it does not qualify as freezing, which requires an equilibrium between the crystalline and liquid state. (wikipedia.org)
  • The tissue was cryopreserved using either slow-freezing or vitrification - a fast-freezing method. (medicalnewstoday.com)
  • Old slow freezing technology generates few vital embryos, but the advanced vitrification method used at SCI Healthcare and SCI IVF Hospital substantially reduces loss rates. (selfgrowth.com)
  • Anyone considering egg freezing needs careful counseling about their age and the odds of success if they want to later thaw those eggs for use in in vitro fertilization, the guidelines stress. (yahoo.com)
  • Think about what often happens when you freeze water into ice cubes and then start to thaw it. (webmd.com)
  • If by the time Wellner gets married the eggs in her ovaries are too old to work, she can thaw out her frozen eggs, have them fertilized with her husband's sperm and implanted into her uterus, and hope that her younger eggs will succeed where her older eggs failed. (cnn.com)
  • Freeze-drying benefits heat-sensitive products by dehydrating in the frozen state without intermediate thaw. (britannica.com)
  • Thaw frozen shrimp under cool running water or in the refrigerator. (ehow.com)
  • There are companies throughout the state that have the ability to thaw frozen services with a re-circulating thawing unit. (constantcontact.com)
  • It is usually recommended that frozen fruits be served before they are completely thawed, since in the partially thawed state the effect of freezing on the fruit tissue is less noticeable. (fao.org)
  • If Williams' son wanted to harvest his father's genes for the highest bidder, he could simply freeze a piece of his father's skin tissue - and save several thousand dollars in the process. (go.com)
  • Frozen shoulder happens when the tissue around your shoulder joint becomes inflamed. (www.nhs.uk)
  • Freezing tissue from the ovary, instead of individual eggs, looks to be a promising alternative. (wired.com)
  • Oktay walked her through the steps: He would remove one of her ovaries, slice pieces from the top tissue layers, and freeze the slices in liquid nitrogen. (thedailybeast.com)
  • In these cases, freezing ovarian tissue, or ovarian tissue cryopreservation, may be an option. (breastcancer.org)
  • Freezing ovarian tissue is experimental - the first baby born using the procedure was in 2004. (breastcancer.org)
  • You may hear about ovarian tissue being transplanted directly from the pelvis to the arm without being frozen. (breastcancer.org)
  • But a new study reveals how deep-freezing testicular tissue may be a promising technique to preserve fertility in these patients. (medicalnewstoday.com)
  • Is Ovarian Tissue Freezing Better Than Egg Freezing? (healthline.com)
  • Freezing tissue is a relatively new procedure. (healthline.com)
  • According to the Alliance for Fertility Preservation , "Ovarian tissue freezing is an experimental technique that involves the freezing and storage of tissue from the ovarian cortex. (healthline.com)
  • A recent study released in Reproductive Sciences found that 37 percent women opting for ovarian tissue freezing were able to successfully conceive. (healthline.com)
  • Dr. Vitaly Kushnir at the Center for Human Reproduction is one of the pioneers in ovarian tissue freezing. (healthline.com)
  • One of the main advantages of ovarian tissue freezing over egg freezing is that it can be performed very quickly, allowing cancer patients to start chemotherapy the next day," he told Healthline. (healthline.com)
  • In that case, ovarian tissue freezing may be. (healthline.com)
  • Additionally," Kushnir explained, "for children with cancer who have not yet reached puberty, ovarian tissue freezing is the only fertility preservation option we have at this time. (healthline.com)
  • But he added, "There is a push in the field to lift the experimental label on ovarian tissue freezing for cancer patients, and this will likely happen in the near future as more data becomes available. (healthline.com)
  • Frozen shoulder is caused by inflammation of the tissue that surrounds the shoulder joint. (news-medical.net)
  • In the case of frozen shoulder, scar tissue forms on the inside of the capsule, causing it to become thick, swollen and tight. (news-medical.net)
  • People with Dupuytren's contracture (a tissue disorder in the hands that causes fingers to bend) are at an increased risk of frozen shoulder. (news-medical.net)
  • First of all, the abdominal or lumbar demarcation of the need for lipolysis, the freezing lipolysis instrument adhered to the skin surface, and subcutaneous tissue cooling to 5 degrees Celsius. (selfgrowth.com)
  • But the technique is still inefficient compared with in vitro fertilization of eggs that have not been frozen, and experts say the procedure should be performed in a controlled, experimental environment. (wired.com)
  • In vitro fertilization with fresh, not frozen, eggs has about a 30 percent birth success rate, while in this study the freezing procedure was 15 percent successful. (wired.com)
  • She couldn't have frozen her eggs, RadioLab 's Webster notes, because the procedure requires weeks-long hormone treatments to induce what RadioLab likened to "20 periods at the same time. (thedailybeast.com)
  • Ovary freezing, on the other hand, is a simple 45-minute outpatient procedure that requires no hormonal preparation. (thedailybeast.com)
  • Freezing women's eggs to treat infertility is no longer an experimental procedure, according to the American Society for Reproductive Medicine. (npr.org)
  • Still, the procedure remains costly and controversial and many questions remain about the effectiveness and safety of using long-term egg-freezing. (npr.org)
  • As of Monday, the procedure of freezing women's eggs is no longer experimental. (npr.org)
  • It's expensive, it doesn't always work, and some have ethical questions about women who use the procedure to effectively stop their biological clock, they have eggs removed and frozen when they're younger for use when they choose. (npr.org)
  • But according to the study authors, "The procedure is superior to egg freezing as it can also reverse menopause and restore natural fertility. (healthline.com)
  • Curious which procedure might be preferred in the future, Healthline reached out to Dr. Aimee Eyvazzadeh, a fertility specialist in California who advocates for preemptive egg freezing. (healthline.com)
  • Egg freezing is no longer an experimental procedure, according to the American Society for Reproductive Medicine (ASRM), which on 22 October issued new guidelines on the controversial practice. (nature.com)
  • The change in policy is expected to accelerate the growth of clinics that offer egg freezing to women who face fertility-damaging treatment for cancer or other conditions, and to women wishing to delay having a baby - although the society stopped short of endorsing the procedure for that purpose. (nature.com)
  • Sperm routinely are frozen. (yahoo.com)
  • But eggs proved more delicate and difficult to freeze than sperm or embryos. (yahoo.com)
  • While men have had the option of freezing their sperm for years, egg freezing was next to impossible less than 20 years ago. (wired.com)
  • While men who undergo chemotherapy or radiation have long had the option of freezing their sperm before treatment to preserve their ability to have children later, the same technology has not been available for women. (wired.com)
  • Sperm is plentiful and easy to freeze. (wired.com)
  • I was told that if I ever hoped to have kids, my options were to freeze my eggs immediately or to find myself a sperm donor and pursue a fresh round of IVF. (healthline.com)
  • Also on the rise is sperm freezing coverage for male employees concerned about their fertility future. (cnbc.com)
  • Boston-based start-up Legacy provides men the option to freeze their sperm. (cnbc.com)
  • Flash freezing being used for cryopreservation . (wikipedia.org)
  • But proponents of egg freezing, known medically as oocyte cryopreservation, say lifting the experimental label will encourage more women to check out the option, and they'll make an educated choice. (yahoo.com)
  • Egg freezing is more technically known as oocyte cryopreservation, a technology by which a woman's eggs are surgically removed from her ovaries and frozen until she is ready to use them. (newsweek.com)
  • Two years ago, the American Society for Reproductive Medicine, an association of infertility specialists, issued an optimistic report about advances in oöcyte cryopreservation-egg freezing, in common parlance. (newyorker.com)
  • Much of the American Midwest is experiencing a deep freeze that will bring with it the lowest temperatures seen in a generation. (pbs.org)
  • Learn how to prep onions for safe storage in the deep freeze. (hgtv.com)
  • AP) - The Upper Midwest remains locked in the deep freeze, with bitter temperatures stretching into a fourth day across several states. (yahoo.com)
  • But now the deep freeze can be found as far south as Chicago's Lake Michigan shoreline. (voanews.com)
  • Freezing is a phase transition where a liquid turns into a solid when its temperature is lowered below its freezing point. (wikipedia.org)
  • [ citation needed ] Because of the latent heat of fusion , the freezing is greatly slowed and the temperature will not drop anymore once the freezing starts but will continue dropping once it finishes. (wikipedia.org)
  • Freezing does not start until the temperature is low enough to provide enough energy to form stable nuclei. (wikipedia.org)
  • This is often seen as counter-intuitive, [9] since the temperature of the material does not rise during freezing, except if the liquid were supercooled . (wikipedia.org)
  • [4] The science of freezing water depends on multiple factors, including how water droplets freeze, how much water is in the atmosphere, if water is in a liquid or crystal state, at what temperature it freezes, and whether it crystallizes from within or from the surface. (wikipedia.org)
  • One can therefore observe a delay until the water adjusts to the new, below-freezing temperature. (wikipedia.org)
  • The temperature plateaus at -10 degrees celsius but the alcohol does not freeze. (yahoo.com)
  • In our news wrap Friday, Washington, New York, Trenton and Baltimore all broke temperature records during a burst of arctic air that wreaked havoc by freezing pipes. (pbs.org)
  • the permissible delay depends on the intrinsic quality of the fish and the temperature of storage prior to freezing. (fao.org)
  • In the case of the beer, opening the bottle reduces the pressure on the liquid and results in a sudden lowering of temperature--causing the unfrozen liquid to freeze. (madsci.org)
  • Amorphous materials, as well as some polymers, do not have a freezing point, as there is no abrupt phase change at any specific temperature. (wikipedia.org)
  • Freeze cooked food as soon as it has cooled to room temperature. (hubpages.com)
  • The phase transitions of water are generally described using natural variables of temperature T and pressure P , which correspond to the Gibbs thermodynamic potential G ( T , P ). Consider the freezing of pure water in an undeformable container (i.e., at constant volume). (nature.com)
  • A related technique, freeze-drying, employs high vacuum conditions, permitting establishment of specific temperature and pressure conditions. (britannica.com)
  • Check the temperature of water, if you suspect it is about to freeze the line. (constantcontact.com)
  • If the temperature is close to freezing, run a substantial amount of water until the temperature increases. (constantcontact.com)
  • Zeltiq freezing fat is converted into solid by triglyceride in human fat at 5 65507 After-low temperature, triglyceride transforms from liquid to solid state, and dies after crystallization and aging . (selfgrowth.com)
  • By freezing the entire ovary, then using it in the manner that these researchers did, they can stimulate it with fertility drugs after the cancer treatment is completed,' Damewood said. (wired.com)
  • After a cancer diagnosis, Annie Dauer decided to undergo a radical new treatment to freeze part of her ovary in hopes of preserving fertility-with amazing results. (thedailybeast.com)
  • rofessor of obstetrics and gynecology at the Yale School of Medicine and one of the preeminent experts of a new process called ovary freezing. (thedailybeast.com)
  • But ovary freezing is poised to challenge their dominance, used most often to preserve the fertility of cancer patients who don't have time to freeze their eggs before undergoing chemotherapy. (thedailybeast.com)
  • Now, almost 20 years after Oktay's pioneering experiment, the science behind ovary freezing has progressed dramatically. (thedailybeast.com)
  • With ovary freezing, the process would cost approximately $18,000, in addition to the yearly storage fee. (thedailybeast.com)
  • One of the biggest differences between egg freezing and ovary freezing, preparation time, was the crux of Annie's case. (thedailybeast.com)
  • With ovary freezing, on the other hand, "you have the source," Oktay told The Daily Beast, which means that a doctor can gather hundreds or even thousands of eggs in various stages of maturity with one surgical removal. (thedailybeast.com)
  • Your ovary or ovaries are removed and strips of them containing unripe eggs are frozen. (breastcancer.org)
  • In egg freezing, women inject themselves with hormones for about two weeks, and then a doctor places a needle into the vagina to retrieve mature egg cells from the ovary. (livescience.com)
  • Egg freezing had long been labeled experimental, but the American Society for Reproductive Medicine declared that's no longer the case. (yahoo.com)
  • That's why the American Society for Reproductive Medicine hasn't endorsed egg freezing, calling it experimental and unproven. (cnn.com)
  • For a number of years, egg-freezing has been offered experimentally for young women or girls who are diagnosed with cancer or other serious illnesses that would destroy their ovaries. (yahoo.com)
  • Egg freezing is similar to in vitro fertilization: Fertility drugs are used to stimulate your ovaries to make multiple mature eggs in one cycle. (webmd.com)
  • Millennial women will not be freezing their ovaries anytime soon. (healthline.com)
  • Freezing may occur at an open doorway (most common), at a line on the floor, or in crowds. (webmd.com)
  • In addition, freezing is observed to occur before or after a fight or flight response. (wikipedia.org)
  • However, in the winter freezes sometimes occur. (prweb.com)
  • Although some authors differentiate solidification from freezing as a process where a liquid turns into a solid by increasing the pressure, the two terms are used interchangeably. (wikipedia.org)
  • Freezing is almost always an exothermic process, meaning that as liquid changes into solid, heat and pressure are released. (wikipedia.org)
  • But this can be understood since heat must be continually removed from the freezing liquid or the freezing process will stop. (wikipedia.org)
  • In physics and chemistry , flash freezing is the process whereby objects are frozen in just a few hours [1] by subjecting them to cryogenic temperatures, or through direct contact with liquid nitrogen at −196 °C (−320.8 °F). It is commonly used in the food industry . (wikipedia.org)
  • American inventor Clarence Birdseye developed the "quick-freezing" process of food preservation in the 20th century. (wikipedia.org)
  • The quality of frozen products and consumer acceptance can be enhanced by optimizing process conditions such as rate of freezing, quality of raw materials, and storage conditions details (see previous chapters for more detail). (fao.org)
  • The analysis used to determine the effects of freezing process, frozen storage, and thawing on product flavor is largely based on the changes produced in chemical compounds. (fao.org)
  • Therefore, a good understanding of the physical aspects of freezing will help improve the product s quality retention during the freezing process. (fao.org)
  • In the process of freezing, when water in the cells freezes, an expansion occurs and ice crystals cause the cell walls to rupture. (fao.org)
  • This quality defect can be prevented by using heavyweight, moisture proof packaging during the freezing process (Pham and Mawson, 1997). (fao.org)
  • Start the freezing process by washing sprouts. (hgtv.com)
  • Some reports Monday said Williams' body had already been frozen, but the process of submerging a body in liquid nitrogen takes several weeks. (go.com)
  • Of course, cryonics has its critics, many of whom contend that the freezing process alone would do irreparable damage to a patient. (go.com)
  • Eggs generally deteriorate during the freezing process because they are not sufficiently dehydrated. (scientificamerican.com)
  • An agricultural friend, at our suggestion, has tried an experiment with a cellar of an out-house, in which on several occasions vegetables have frozen, although the cellar was fortified against fj'ost by a process known to farmers as "banking. (scientificamerican.com)
  • The supercooled liquid will freeze rapidly on the addition of a crystal of sodium thiosulfate, or on stirring which seeds the crystallisation process. (rsc.org)
  • The freezing process preserves nutrients and provides a fresher flavor than canning or drying foods. (osu.edu)
  • Wellner's 13 eggs might not survive the freezing process. (cnn.com)
  • Using a process called directional freezing, you can make a huge batch of perfectly clear ice without any expensive equipment. (lifehacker.com)
  • As the video explains, the cloudiness you see in most ice is formed toward the end of the freezing process. (lifehacker.com)
  • Directional freezing in an insulated cooler- a process originally developed by Camper English at Alcademics -forces the water to freeze top to bottom, which keeps the water in the cooler from freezing all the way through. (lifehacker.com)
  • Besides the (usually minor) health risks women may face from the process itself, there's another possible drawback: freezing your eggs does not guarantee you will one day have a baby. (newsweek.com)
  • We conclude that HDA6 plays a critical role in regulating cold acclimation process that confers freezing resistance on Arabidopsis. (nih.gov)
  • Water dripping from a slab of ice and then freezing, forming icicles . (wikipedia.org)
  • When water is supercooled to temperatures below −48 °C (−54 °F), it must freeze. (wikipedia.org)
  • Freezing water is a central issue for climate, geology and life. (wikipedia.org)
  • The freezing of nanoscale water or silicon liquid drops is initiated at a number of different distances from the centre of the droplet, providing new insights on a long-standing dispute in the field of material and chemical physics . (wikipedia.org)
  • [5] The resulting ice depends on how quickly the system is cooled: If the water is cooled below its freezing point slowly, an ice crystal will result, rather than the poly-crystalline solid that flash freezing produces. (wikipedia.org)
  • There are phenomena like supercooling, in which the water is cooled below its freezing point, but the water remains liquid, if there are too few defects to seed crystallization. (wikipedia.org)
  • Ideally the fish should be held in chilled sea water prior to freezing but, where this facility is not fitted, ice can be used as an alternative. (fao.org)
  • If you leave water out for your neighborhood cats, take a look at these tips for preventing freezing water in the cold months. (petfinder.com)
  • The problem: Eggs contain lots of water, and early methods of freezing and thawing allowed ice crystals to form that could destroy or damage them. (yahoo.com)
  • Wash berries in ice cold water, drain and dry in a clean tea towel before freezing in one of the above mentioned methods. (pioneerthinking.com)
  • Because water in vegetables expands during freezing and breaks cell walls, thawed vegetables might "leak" and have a soft texture. (osu.edu)
  • When defrosting frozen food make sure you defrost in the refrigerator, in cold water or in the microwave. (hubpages.com)
  • Exposed water pipes will freeze, the agency said. (latimes.com)
  • In fact, you don't want the water to freeze all the way through because that's when the impurities begin to form. (lifehacker.com)
  • Water expands upon freezing. (nature.com)
  • One phase transformation of emerging importance is the freezing of water in an undeformable, constant-volume container. (nature.com)
  • Given this recent interest, a robust, multi-scale theoretical treatment of the thermodynamics and kinetics of freezing water in isochoric (constant-volume) systems is needed. (nature.com)
  • Water expands upon freezing to ice-1h, and should the container have a lower specific volume than that of ice-1h, the contents of the system will never be able to freeze entirely, resulting in a two-phase water-ice equilibrium. (nature.com)
  • Since a frozen food has a very small amount of unfrozen water that attracts the microwave energy first, the heat is generated in a small part of the food load and is rapidly absorbed by the frozen part. (encyclopedia.com)
  • Freeze-drying of food to preserve it involves sublimation of water from the food in a frozen state under high vacuum. (britannica.com)
  • Sherbet , frozen dessert usually flavoured with fruit, made from water, sugar , flavourings, and milk or cream . (britannica.com)
  • Leaving the water running at a trickle can help prevent frozen water service lines. (constantcontact.com)
  • Trickling water can sometimes lead to frozen sewer service lines. (constantcontact.com)
  • Trickling water in a sewer line can cool to the point of freezing if the sewer line is cold. (constantcontact.com)
  • If the consumer having a freezing water issue is a rural customer with a septic system, trickling water should be diverted away from the septic system. (constantcontact.com)
  • If the water service line does become frozen advise the customer to open one or two faucets. (constantcontact.com)
  • Fish was first frozen in the United States in 1865, and in 1917, Clarence Birdseye began word on freezing foods for retail trade. (encyclopedia.com)
  • Now, women have become so hopeful that egg freezing will help them preserve their fertility that many are paying $400 per year to have their eggs frozen by Extend Fertility , a company that purports to help women stop the ticking biological clock - a fertility insurance policy. (wired.com)
  • Fast freezing yields a fresher, less mushy product. (hgtv.com)
  • If the fish is already borderline, freezing it won't make it any fresher, and you'll want to eat it right away after thawing. (ehow.com)
  • While freezing slows down spoilage, when the food is thawed, the growth of bacteria, yeasts or mold will continue. (osu.edu)
  • Freezing eggs for one's own future use has become a growing trend, with an estimated 5,000 women nationwide making use of the technology, according to Dr. Daniel Shapiro, medical director of the egg bank at Reproductive Biology Associates in Atlanta. (cnn.com)
  • What if electively freezing one's eggs is not a means of empowerment but a surrender to corporate control? (newyorker.com)
  • What if, rather than being a means of empowerment-whereby a young woman is no longer subject to anything so quaintly analog as the ticking of a biological clock-freezing one's eggs is understood as a surrender to the larger, more invisibly pervasive force of corporate control? (newyorker.com)
  • Adams started a web site, eggsurance.com, to spread word of women's experiences with egg freezing. (yahoo.com)
  • Extend had just begun marketing egg freezing as the newest choice among women's options: preserve your fertility and wait to have a child. (newsweek.com)
  • There are several tricks you can learn to help you become "unfrozen" when a freezing episode occurs. (webmd.com)
  • The risk for precipitation goes up this evening, and it could start as a very few snowflakes before quickly transitioning to light sleet and freezing rain. (washingtonpost.com)
  • The predominant type of precipitation overnight should be freezing rain until well north of the city where more sleet is possible. (washingtonpost.com)
  • Tuesday could bring a combination of freezing snow and sleet, meteorologist Charles Mott said. (chicagotribune.com)
  • One option is to have your eggs harvested and fertilized -- just as women who get in vitro fertilization ( IVF ) do -- but instead of having them implanted, resulting embryos are frozen and stored. (webmd.com)
  • The embryos are frozen for use in a future cycle instead of transferring the embryos back into the uterus in the same cycle. (selfgrowth.com)
  • New Mexico is also facing below-freezing temperatures, while the high in Albuquerque will be at or below freezing for the next three days. (latimes.com)
  • Albuquerque saw below-freezing temperatures Friday night. (latimes.com)
  • Freezing Brussels sprouts successfully starts with a homegrown or locally raised product. (hgtv.com)
  • The development of appliances with freezing sections that had separate compressors that allowed the freezing section to successfully hold frozen foods in the frozen condition had a major impact on the consumer's ability to store food. (encyclopedia.com)
  • They found that 62 percent of women who freeze their eggs at age 35 and try to get pregnant at age 40 would successfully have a baby, with the average total cost of the procedures leading to the birth coming to $39,946. (livescience.com)
  • Because ovarian freezing and transplantation are experimental, it's hard to estimate how much the procedures cost. (breastcancer.org)
  • The thermodynamics of freezing and melting is a classical discipline within physical chemistry, [4] which nowadays develops in conjunction with computer simulations. (wikipedia.org)
  • As mentioned earlier, several enzymes that cause the loss of color, loss of nutrients, flavor changes, and color changes in frozen fruits and vegetables, should be inactivated by means of thermal treatments prior to freezing. (fao.org)
  • The USDA and land grant universities responded to this concern with research studies to assess the impact of freezing on nutrients in fruits, vegetables, and meat, which are summarized by Karmas and Harris. (encyclopedia.com)
  • Freezing most often affects walking, but it also can affect speech, writing, and the person's ability to open and close his or her eyes . (webmd.com)
  • Freezing in the Dark: Why Canada Needs Strategic Petroleum Reserves points out the precariousness of current global oil supplies, especially given current tensions in the Middle East, and fact that Canada imports close to 1 million barrels of oil per day to supply the needs of central and eastern provinces. (greenpeace.org)
  • In temperatures close to freezing and waves eight meters high, the Italian coast guard took the migrants on board their small patrol vessels. (voanews.com)
  • What's involved in freezing eggs: Women inject high levels of hormones for a week in order to ovulate as many eggs as possible. (yahoo.com)
  • Even though freezing foods inactivates yeast, bacteria and mold, once the food is thawed they can once again become active. (hubpages.com)
  • If you ever buy chicken and freeze some to use later, here's a simple trick that's better than brining for turning out juicier meat: Salt the chicken before you freeze it. (lifehacker.com)
  • Treating the chicken before freezing works better than after because it gives the salt additional time to do its job-while the meat is freezing and then again while it is thawing. (lifehacker.com)
  • Salting the chicken before freezing works better than brining since salting creates a more concentrated brine at the surface of the meat, allowing more salt to travel into the meat. (lifehacker.com)
  • Freeze-drying of meat yields a product of excellent stability, which on rehydration closely resembles fresh meat. (britannica.com)
  • Andean peoples developed preserves of freeze-dried meat, fish, and mealy tubers ( charki , chuñu ) that kept indefinitely and weighed much less than the original food. (britannica.com)
  • For a whole salmon, clean the meat before freezing salmon. (ehow.com)
  • Freezing foods retards the growth of the microorganisms and slows down chemical changes that might cause food to spoil. (osu.edu)
  • Freezing slows down the deterioration of food it does not stop it. (hubpages.com)
  • WASHINGTON (AP) - Freezing human eggs can be successful in treating infertility - but guidelines issued Friday still urge caution for women hoping to pause a ticking biological clock. (yahoo.com)
  • Is the Catholic Church against freezing human eggs to be used at a later date? (ewtn.com)
  • The egg-freezing study has particular significance for Italian women wanting to use in vitro fertilization to get pregnant. (wired.com)
  • By 2012, the evidence was in: scientists determined that, for young women, eggs that have been frozen using these methods are just as likely to result in pregnancy and the birth of a healthy child as ones freshly harvested in a cycle of in-vitro fertilization. (newyorker.com)
  • The group cited studies that found younger women are about as likely to get pregnant if they used frozen-and-thawed eggs for their infertility treatment as if they used fresh ones. (yahoo.com)
  • Here's the controversy: Should otherwise healthy women freeze their eggs as sort of an insurance policy against infertility in case they don't meet Mr. Right - or just aren't ready for motherhood - until their late 30s or beyond, when the childbearing window is closing fast? (yahoo.com)
  • Yet the society that represents doctors who treat infertility stopped short of endorsing egg freezing solely for deferring childbearing until women are older. (yahoo.com)
  • Women may soon have an option that has long been available to men: to freeze their reproductive tissues for future pregnancies. (wired.com)
  • Doctors have tried giving women fertility drugs to produce more eggs before chemo or radiation, then freezing them, without much success. (wired.com)
  • For women at risk of losing their fertility through medical treatments or premature menopause, preserving some of their eggs ahead of time by freezing them can leave open the possibility of having children. (scientificamerican.com)
  • Today, egg freezing is on the rise, with Time magazine recently reporting that 76,000 women will freeze their eggs by 2018. (healthline.com)
  • But for women facing a health crisis that threatens to strip them of their fertility, egg freezing is no longer the only fertility preservation option. (healthline.com)
  • This time, when I go back to see Noyes, she tells me that since we last talked she had done 17 egg-freezing cycles in women up to age 38. (newsweek.com)
  • For women who may want to have children in their 40s, freezing their eggs in their 20s or 30s costs less and has a higher success rate than waiting until age 40 to try to get pregnant, a new study finds. (livescience.com)
  • Egg freezing remained the more cost-effective option for women up until age 38, the researchers said. (livescience.com)
  • Some women are beginning to embrace egg freezing in order to keep open the possibility of having kids in their 40s, Dr. Kate Devine of the National Institutes of Health and her colleagues wrote in their study. (livescience.com)
  • Under a third scenario, women freeze their eggs at age 35, and then at age 40, they try conventional IVF. (livescience.com)
  • Egg freezing is not usually covered by insurance, so women must typically pay for it themselves, Vitek said. (livescience.com)
  • For example, the authors assumed that live birth rates from frozen eggs would be similar to birth rates using eggs that had just been retrieved, but researchers do not know if this would hold true for eggs that were retrieved from women who are nearing the end of their reproductive years, she said. (livescience.com)
  • As millennial women enter their 30s, demand for customized and affordable egg freezing services is spiking. (cnbc.com)
  • Marika Frumes, 35, is among the growing number of millennial women opting to freeze their eggs, and now organizes events to educate others. (cnbc.com)
  • As women delay motherhood and take their time in finding the right partner, fertility experts see egg freezing becoming more popular. (cnbc.com)
  • There are more women who are single professionals with disposable income who are now pursuing egg freezing in the U.S.,' Gina Bartasi, CEO of Kindbody , told CNBC. (cnbc.com)
  • The topic will be whether younger women should consider freezing their eggs for the future, says Pfeifer. (nature.com)
  • In the past five years, however, more refined freezing methods have been developed and made available to some women whose fertility has been compromised by chemotherapy or premature menopause. (newyorker.com)
  • To such women, egg freezing might seem to offer liberation from those wearying dictates of biology by which their older sisters, no matter how successful their careers, were bound. (newyorker.com)
  • Lots of women have chanced it, even before egg freezing came along and supplied a possible, if not entirely reliable, form of counter-infertility insurance. (newyorker.com)
  • Flash freezing techniques are used to freeze biological samples quickly so that large ice crystals cannot form and damage the sample. (wikipedia.org)
  • While the successful freezing of human semen and embryos had been possible for decades, eggs proved much more fragile, prone to forming ice crystals that rendered them unviable. (newyorker.com)
  • Many were unprepared for the freeze and did not drip their faucets properly, resulting in their pipes freezing up. (prweb.com)
  • Now with more freeze warnings coming up, Houstonians need to know that John Moore will be available if they have busted pipes, even after hours. (prweb.com)
  • This was never more needed when a freeze went through Houston in January 2010 and many residents found out their pipes busted because they froze up. (prweb.com)
  • The National Weather Service warned residents in those areas to take steps to prevent pipes from freezing such as wrapping them or allowing faucets to drip slowly. (ap.org)
  • 2. Wait until fruit is completely cold before packaging and freezing. (pioneerthinking.com)
  • It's done by putting a very cold chemical on the cells to freeze them and then remove them. (plannedparenthood.org)
  • Put your salmon in the coldest areas when freezing salmon to keep it as cold and frozen as possible. (ehow.com)
  • Houstonians are not used to cold weather and when a freeze develops, they usually go on as they normally do in the spring and summer. (prweb.com)
  • A blast of cold air drove temperatures below freezing in many southern states. (ap.org)
  • Many plants exhibit altered gene expression patterns in response to low nonfreezing temperatures and an increase in freezing tolerance in a phenomenon known as cold acclimation. (nih.gov)
  • Here we show, for the first time, that the histone deacetylase gene HDA6 is required for cold acclimation and freezing tolerance in Arabidopsis. (nih.gov)
  • Cold-treated hda6 mutants showed reduced freezing tolerance compared with the cold-treated wild-type plants. (nih.gov)
  • Freezing-caused electrolyte leakage increased in the cold-treated hda6 mutant. (nih.gov)
  • The simple fat reduction technique harnesses the cold, freezing fat cells and encouraging them to break down permanently. (prnewswire.com)
  • For example, agar displays a hysteresis in its melting point and freezing point. (wikipedia.org)
  • Cook's Illustrated conducted an experiment comparing moisture loss before and after freezing. (lifehacker.com)
  • Preserve that tangy flavor by freezing celery-we'll explain how. (hgtv.com)
  • Excess air leads to flavor loss or off-flavors in frozen foods. (osu.edu)
  • The closer to peak of freshness you freeze the food, the better the flavor. (hubpages.com)
  • Frozen cooked shrimp does not retain flavor as well, but it freezes with ease. (ehow.com)
  • This is the main reason for eliminating any delays during pre-freezing operations. (fao.org)
  • The trans-Siberian railway, Russia's main east-west link, was out of action last weekend after stretches of rail had fractured in the freeze. (economist.com)
  • These early models were great successes, and they launched the freezing preservation of fruits and vegetables in this country. (encyclopedia.com)
  • The possibility of freezing preservation of fruits and vegetables became an important interest in the United States , catching the attention of many city apartment dwellers and suburban families who lived in small houses. (encyclopedia.com)
  • Questions were raised about the effect of freezing meats, fruits, and vegetables on the quality of the thawed product, including its nutrient retention. (encyclopedia.com)
  • Of these, some are transferred into the woman s uterus, while the others are frozen for future use. (ewtn.com)
  • Although freeze-drying produces a food of outstanding quality, the cost is high, and it has not been used widely in vegetable products. (britannica.com)
  • If you do it when you're 30, there's about a 13.2% chance of getting pregnant in a given cycle, compared with just 8.6% with eggs frozen at 40. (webmd.com)
  • Then she relaxed as she remembered what awaited her 50 blocks away: 13 eggs frozen in a bank at New York University Fertility Center . (cnn.com)
  • Meanwhile, egg freezing typically requires several weeks of hormonal treatments before the actual collection of eggs is performed. (healthline.com)
  • For Brigitte Adams of San Francisco, that hope of a future pregnancy was worth paying about $15,000 to freeze 11 eggs, especially when her parents covered half the bill. (yahoo.com)
  • Your ultimate odds of a successful pregnancy are greater if you're younger when you freeze your eggs. (webmd.com)
  • Shapiro at RBA estimates that a woman who freezes her eggs between the ages of 32 and 35 will have a 40 to 50% chance of achieving a successful pregnancy. (cnn.com)
  • Generally, pregnancy probability is higher for frozen embryos than embryos transferred during a fresh IVF cycle. (selfgrowth.com)
  • Moisture migration is the principal physical change occurring in frozen foods, affecting the physical, chemical, and biochemical properties, including texture and palatability of the food (Pham and Mawson, 1997). (fao.org)
  • You will find that half the normal cooking time is ample for fruit which is to be frozen. (pioneerthinking.com)
  • Rafaella Fabbri of the University of Bologna and her colleagues found that increasing the amount of time the eggs were exposed to chemical protectants and boosting the amount of sugar in the freezing solution improved dehydration. (scientificamerican.com)
  • a) last time Israel''s government froze construction, nothing happened. (jpost.com)
  • Starting Monday, July 2, new IPOs will be released at an unspecified time each Monday, Wednesday and Friday morning, and traders will be able to buy or short the new propositions… but trades placed throughout the day will be frozen until late afternoon , when the stock will be released into play. (popsci.com)
  • With a single click of button you can stop them all and freeze time! (steampowered.com)
  • The canny nostalgia play of Frozen arrives just in time for Thanksgiving, catering not just to kids but also the parents who grew up quoting dialogue and humming tunes from Beauty and the Beast , The Little Mermaid , The Lion King and Aladdin . (pastemagazine.com)
  • Freezing behavior is most easily characterized by changes in blood pressure and lengths of time in crouching position, but it also is known to cause changes such as shortness of breath, increased heart rate, sweating, or choking sensation. (wikipedia.org)
  • At the time, egg freezing was still somewhat new, and the success rates weren't great. (healthline.com)
  • Egg freezing parties have become a thing, with millennials opting to preserve their eggs, buying themselves more time to find a partner and pursue a career. (healthline.com)
  • Each time a "background" operation was executed, the draktools seemed to freeze. (osnews.com)
  • Early use of freezing occurred in parts of the world, such as Canada , where temperatures in winter drop below freezing for significant periods of time. (encyclopedia.com)
  • Using extreme ultraviolet light bursts lasting 0.0000000000000002 seconds - that´s 200 quintillionths of a second - Arvinder Sandhu and his team have managed to freeze the unimaginably fast action that ensues when oxygen molecules are zapped with high energies for incredibly short amounts of time. (redorbit.com)
  • Most of the time, there is no cause for frozen shoulder. (medlineplus.gov)
  • Freeze shrimp properly to store it for the maximum amount of time. (ehow.com)
  • Once you properly freeze the shrimp, use it within the recommended storage time to prevent a loss in quality. (ehow.com)
  • It's none of our business to tell someone, 'No, you shouldn't delay childbearing if you choose for whatever reason,'" said Dr. James Grifo of New York University, whose center has frozen more than 1,100 batches of eggs, mostly for elective fertility preservation. (yahoo.com)
  • they are not sufficiently moisture-resistant and vapor-resistant for long-term frozen food storage. (osu.edu)
  • Once they're frozen, wrap them for storage. (ehow.com)
  • But if you find you have an excess of rhubarb, you can easily freeze the leftovers for extended storage. (wikihow.com)
  • SIRM now has a FREE Egg Freezing program for Cancer Patients - Feel free to ask your questions here. (inciid.org)
  • But in Sioux Falls, S.D., where winter temperatures are normally well below freezing, some homeless shelters had open beds. (yahoo.com)
  • I recommend the flat pack method of packaging sugared fruits in meal-sized quantities or use any of the special plastic or wax coated containers with tightly fitting lids to seal, label and freeze. (pioneerthinking.com)
  • When freezing foods, use containers and packing materials that are durable, easy to seal, resist cracking at low temperatures, and are moisture- and vapor-resistant. (bhg.com)
  • [10] Helium-3 has a negative enthalpy of fusion at temperatures below 0.3 K. Helium-4 also has a very slightly negative enthalpy of fusion below 0.8 K. This means that, at appropriate constant pressures, heat must be added to these substances in order to freeze them. (wikipedia.org)