Sex differences in the effects of early neocortical injury on neuronal size distribution of the medial geniculate nucleus in the rat are mediated by perinatal gonadal steroids. (1/2521)

Freezing injury to the cortical plate of rats induces cerebrocortical microgyria and, in males but not females, a shift toward greater numbers of small neurons in the medial geniculate nucleus (MGN). The purpose of the current study was to examine a hormonal basis for this sex difference. Cross-sectional neuronal areas of the MGN were measured in male rats, untreated female rats and female rats treated perinatally with testosterone propionate, all of which had received either neonatal cortical freezing or sham injury. Both male and androgenized female rats with microgyria had significantly smaller MGN neurons when compared to their sham-operated counterparts, whereas untreated females with microgyria did not. These differences were also reflected in MGN neuronal size distribution: both male and androgenized female rats with microgyria had more small and fewer large neurons in their MGN in comparison to shams, while there was no difference in MGN neuronal size distribution between lesioned and sham females. These findings suggest that perinatal gonadal steroids mediate the sex difference in thalamic response to induction of microgyria in the rat cortex.  (+info)

Improved antibody detection by the use of range expansion and longer filter wavelength in a low ionic strength-protamine sulphate Auto-Analyzer system. (2/2521)

Range expansion, achieved by insertion of a variable resistance between the colorimeter and the recorder together with the use of 550 nm colorimeter filters, has resulted in markedly improved sensitivity for antibody detection, and improved sample identification, in a low ionic strength-protamine sulphate (LISPS) system. Range expansion also permits a lower concentration of red cells to be used, thus economizing on fully typed cells. Glycerol stored frozen cells were found to be only slightly less sensitive than fresh cells in this system.  (+info)

Cerebral blood flow in the monkey after focal cryogenic injury. (3/2521)

A focal cryogenic lesion was made in the left superior frontal gyrus of the anesthetized macaque brain. Cerebral blood flow (CBF) was determined by the hydrogen clearance technique before and during the 4 hours following trauma. Local CBF in tissue adjacent to the lesion increased in the first half hour after the lesion was made and then decreased during the ensuing 3 1/2 hours. Local CBF in the contralateral superior frontal gyrus, as well as total CBF and oxygen consumption, were unchanged by cryogenic trauma. The spread of vasogenic edema into uninjured tissue probably accounts for the observed decrease in local CBF. This experimental model may assist in discovering therapy to alter favorably the spatial and temporal profile of pathologic CBF changes in tissue surrounding an acute lesion of the brain.  (+info)

Preferential acid-catalyzed hydrolysis of the formamide linkage of N'-formylkynurenine in frozen solution. (4/2521)

Acid-catalyzed hydrolysis of the formamide linkage of N-acetyl-N'-formyl-L-kynurenineamide in frozen dilute hydrochloric acid solution followed first-order kinetics, yielding N-acetyl-L-kynurenineamide as the sole reaction product. The maximum rate of reaction in the frozen solution was found at around -7.5 degrees and approximated that of the reaction in liquid solution at 40 degrees. By freezing the dilute acid solution at -8 degrees the reaction was accelerated by 60 times compared with that in super-cooled liquid solution at the same temperature.  (+info)

A bioluminescence method for the mapping of local ATP concentrations within the arterial wall, with potential to assess the in vivo situation. (5/2521)

According to the anoxemia theory of atherosclerosis, an imbalance between the demand for and supply of oxygen and nutrients in the arterial wall is a key factor in atherogenesis. However, the energy metabolic state of the arterial tissue in vivo is largely unknown. We applied a bioluminescence method, metabolic imaging, to study local ATP concentrations in cryosections of normal pig and atherosclerotic and normal rabbit aorta. Some vessels were subjected to energy metabolic restrictions by incubation at different oxygen and glucose concentrations and others were rapidly frozen in liquid nitrogen to reflect the in vivo situation. Local ATP concentrations and the ATP distribution at a microscale was dependent on oxygen as well as glucose concentrations during incubation. ATP depletion was seen in the mid media of pig aorta in all incubations, but only at low oxygen concentration without glucose in the media of the thinner rabbit aorta. ATP-depleted zones were seen deep in pig media (>750 microm from the lumen) and in rabbit plaques (>300 micrometer+ from the lumen) even at high oxygen (pig 75% O2 and rabbit 21% O2) and glucose concentrations (5.6 mmol/L glucose). This observation probably illustrates an insufficient diffusion of glucose, which highlights the importance of studying the conditions for diffusion not only of oxygen but also of other metabolites in the arterial wall. In rapidly frozen vessels the medial ATP concentration was shown to be 0.6 to 0.8 micromol/g wet weight (both pig and rabbit aorta) and in pig aorta a gradient could be seen indicating higher ATP concentrations at the lumenal side. We propose that metabolic imaging, as applied to snap-frozen tissue, may be used to assess the energy metabolic situation in the arterial wall in vivo. The spatial resolution allows the detection of local variations within the arterial tree. However, steep concentration gradients (eg, near the border of the tissue) will be underestimated. The method may be extended to include determinations of glucose and lactate concentrations and will be used in parallel with an established method to assess hypoxia in the arterial wall in vivo.  (+info)

Changes in allograft bone irradiated at different temperatures. (6/2521)

Secondary sterilisation of allograft bone by gamma irradiation is common, but the conditions under which it is performed vary between tissue banks. Some do so at room temperature, others while the bone is frozen. Bone is made brittle by irradiation because of the destruction of collagen alpha chains, probably mediated by free radicals generated from water molecules. Freezing reduces the mobility of water molecules and may therefore decrease the production of free radicals. We found that bone irradiated at -78 degrees C was less brittle and had less collagen damage than when irradiated at room temperature. These findings may have implications for bone-banking.  (+info)

Rough surfaced smooth endoplasmic reticulum in rat and mouse cerebellar Purkinje cells visualized by quick-freezing techniques. (7/2521)

The in vivo structure of the smooth endoplasmic reticulum (ER) was visualized in rat and mouse cerebellar Purkinje cells by using quick-freezing techniques followed by freeze-substitution for ultrathin-sectioning or freeze-fracturing and deep-etching for replicas. High magnification electron microscopy of the ultrathin sections revealed a surprising finding that all the smooth ER are apparently rough surfaced, and heavily studded with a large number of small dense projections. In the soma the smooth ER appears to be similar to its rough counterpart, except that the projections are slightly smaller, less electron dense and less protrusive on the ER membranes than the ribosomes. The projections were short rectangles, 20 x 20 x 6 nm3 in size, covering the cytoplasmic surface of the smooth ER in a checker-board manner where closely packed. After freeze-etching and replication, they appeared to be composed of four subparticles, surrounding a central channel. Thus the projections are very similar to the foot structure (ryanodine receptor) of the sarcoplasmic reticulum. Furthermore, they were distributed exclusively in the ER compartment and were highly concentrated especially in the smooth ER. This localization of the projections coindides with the intracellular distribution of the inositol 1,4,5-trisphosphate (IP3) receptor determined by quantitative immunogold electron microscopy. These findings would suggest that the projections are tetramers of IP3 receptor molecules and could be used as a morphological marker for the smooth ER in Purkinje cells, which spreads from the soma to the axon and dendrite, up to the tips including the spines. In Purkinje cells tubular smooth ER runs freely in a serpentine fashion or are intertwined to make large membraneous tangles without forming cisternal stacks. It is highly probable that the ER cisternal stacks do not exist naturally in Purkinje cells but are formed artificially during the various procedures for chemical fixation.  (+info)

A leucine-rich repeat protein of carrot that exhibits antifreeze activity. (8/2521)

A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs.  (+info)

Effect of lysed platelet count in platelet concentrates on various growth factor levels after freeze thaw cycles<...
TY - JOUR. T1 - Effect of lysed platelet count in platelet concentrates on various growth factor levels after freeze thaw cycles. AU - Nugraha, Yudhi. AU - Sari, Pudji. AU - Purwoko, Reza Yuridian. AU - Luviah, Evah. AU - Adiwinata, Jeanne. PY - 2014/1/1. Y1 - 2014/1/1. N2 - Platelet concentrate (PC) has been used as substitute to the use of fetal bovine serum (FBS) in cell culture media. However, its use as additive in cell culture media showed inconsistent results on cell proliferation, and the inconsistent results may be due to variability of platelet counts or growth factor content. Standard protocols are lacking for the preparation of PC before it is applied as additive in cell culture media. The growth factor content of PC can be released by freeze-thaw cycles, which range from one to three cycles before use for cell culture media. This study aimed to measure base-line platelet counts and growth factor levels and compare to platelet counts and growth factor levels after one, two and three ...
https://scholar.ui.ac.id/en/publications/effect-of-lysed-platelet-count-in-platelet-concentrates-on-variou
Behavior of FRP bonded to steel under freeze thaw cycles<...
TY - JOUR. T1 - Behavior of FRP bonded to steel under freeze thaw cycles. AU - Toufigh, Vahab. AU - Toufigh, Vahid. AU - Saadatmanesh, Hamid. PY - 2013/1. Y1 - 2013/1. N2 - Fiber reinforced polymers (FRP) materials are increasingly being used for strengthening and repair of steel structures. An issue that concerns engineers in steel members which are retrofitted with FRP is stress experienced due to temperature changes. The changing temperature affects the interface bond between the FRP and Steel. This research focused on the effects of cyclical thermal loadings on the interface properties of FRP bounded to steel members. Over fifty tests were conducted to investigate the thermal effects on bonding between FRP and steel, which were cycled from temperature of -11°C (12°F) to 60°C (140°F) for 21-36 days. This investigation consisted of two test protocols, 1) the tensile test of epoxy resin, tack coat, FRP and FRP-steel plate, 2) tensile test of each FRP compound and FRP with steel after going ...
https://arizona.pure.elsevier.com/en/publications/behavior-of-frp-bonded-to-steel-under-freeze-thaw-cycles
Repeated freeze thaw cycles do not affect the ELISA res | Open-iRepeated freeze thaw cycles do not affect the ELISA res | Open-i
Repeated freeze thaw cycles do not affect the ELISA response.One mL aliquots of either total Sloop hordein (1.0 mg/mL; in a solution containing 8 M urea, 1% (w/
https://openi.nlm.nih.gov/detailedresult.php?img=PMC3585327_pone.0056456.g009&req=4
The catalytic activity and penicillin sensitivity in the liquid and frozen states of membrane-bound and detergent-solubilised...The catalytic activity and penicillin sensitivity in the liquid and frozen states of membrane-bound and detergent-solubilised...
en] The Km, app. values of the membrane-bound transpeptidase of Streptomyces R61 for the donor Ac2-L-Lys-D-Ala-D-Ala and the acceptor Gly-Gly are not affected by temperature variations when the reaction mixtures are incubated in liquid suspensions. At -5 degrees C, the incubation can be carried out either in the liquid or in the frozen state. The enzyme is active in the latter state. In the frozen state, the Km, app. value for the acceptor remains unchanged but there is a 3-fold increase in the maximum velocity, a 10-fold decrease of the Km, app. value for the donor and a 10-fold increase of the benzylpenicillin concentration required to inhibit the enzyme activity by 50% (ID50 value). Temperatures of -35 degrees C or below are required to completely inhibit the membrane-bound enzyme in the frozen state. Cetyltrimethylammonium bromide extracts the transpeptidase both from the isolated membranes and, with a much higher yield, from the intact mycelium. The extracted enzyme is not active in the ...
https://orbi.uliege.be/handle/2268/83083
Scrambled AAV siRNA Control Virus (Serotype 3)
AAV Viruses are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles. ...
https://www.abmgood.com/scrambled-aav-sirna-control-virus-serotype-3-iaav01503.html
Scrambled AAV siRNA Control Virus (Serotype 9)
AAV Viruses are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles. ...
https://www.abmgood.com/scrambled-aav-sirna-control-virus-serotype-9-iaav01509.html
Most recent papers with the keyword Microgyria | Read by QxMDMost recent papers with the keyword Microgyria | Read by QxMD
AIM: The effects on neural repair of intraparenchymal nerve growth factor (NGF) administration were evaluated in neonate Wistar rats with experimentally induced focal microgyria. METHODS: A freezing focal polymicrogyric lesion was induced on the frontal cortex in 35 newborn Wistar rats on postnatal day 1. NGF was administered in 15 cases, with 20 pups as controls. Animals were sacrificed at 72 h and 7 days after NGF administration. Real-time PCR was used for the quantification of the expression of TrkA, p75, and doublecortin (DCX) at the level of the cortical lesion in seven different groups of animals: control 72 h (n = 5), control 7 days (n = 5), microgyria 72 h (n = 5), microgyria 7 days (n = 5), microgyria + NGF 72 h (n = 5), microgyria + NGF 7 days (n = 5), and control + NGF (n = 5 ...
https://www.readbyqxmd.com/keyword/5289
Article | Effect of combining high-pressure processing and frozen storage on the functional and sensory properties of horse...
Frozen storage of horse mackerel (Trachurus trachurus) is limited by lipid damage causing sensory quality losses. This work deals with changes in functional and sensory properties during frozen storage of horse mackerel treated by high hydrostatic pressure processing (HPP) prior to freezing. Three levels of pressure (150, 300, and 450 MPa), pressure holding time (0.0, 2.5, and 5.0 min), and frozen storage time (0, 1, and 3 months) were studied. Expressible water, colour parameters, mechanical texture parameters and sensory parameters were evaluated in raw and cooked samples. The texture profile analysis of raw and cooked HPP samples suggested that a product texture similar or close to fresh muscle is possible. The sensory analysis showed that a 150 MPa treatment yielded high acceptability values. Although acceptability decreased during frozen storage, values remained close to those of fresh samples ...
https://ir.library.oregonstate.edu/concern/articles/wd375x94q
ASTM D7792 / D7792M - 15 Standard Practice for Freeze/Thaw Conditioning of Pultruded Fiber Reinforced Polymer (FRP) Composites...ASTM D7792 / D7792M - 15 Standard Practice for Freeze/Thaw Conditioning of Pultruded Fiber Reinforced Polymer (FRP) Composites...
D7792 / D7792M-15 Standard Practice for Freeze/Thaw Conditioning of Pultruded Fiber Reinforced Polymer (FRP) Composites Used in Structural Designs test specimens~ freeze-thaw cycle~ structural design~ fiber reinforced polymer~
https://www.astm.org/Standards/D7792.htm
Freeze-Thaw Risk: Material Testing | Building Science CorporationFreeze-Thaw Risk: Material Testing | Building Science Corporation
Back to Recommended Freeze-Thaw Risk Assessment Steps. In many projects, the risk is not clear from inspection alone. Material testing is the next step in evaluating risk. Samples are removed from the building being assessed and shipped to our lab.. Lab tests can include dry density, A-value (absorption or liquid water uptake rate), and saturation moisture content (storage). However, the primary method used by BSC to assess the risk of freeze-thaw damage is frost dilatometry. Frost dilatometry, as first developed by Fagerlund1, involves putting thin slices of brick through multiple freeze-thaw cycles until damage is measurable. This process produces a measure of Scrit (the Critical Degree of Saturation) - the point of saturation at which a given masonry material will fail. By estimating the typical degree of saturation for a building, and comparing it to Scrit, the risk of freeze-thaw damage can be predicted. Freeze-thaw cycling below Scrit has low risk of damage, but above Scrit, damage can ...
https://www.buildingscience.com/documents/special/freeze-thaw-documents/risk-assessment-page2
Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues<...Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues<...
TY - JOUR. T1 - Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. AU - Teo, Ka Yaw. AU - DeHoyos, Tenok O.. AU - Dutton, J. Craig. AU - Grinnell, Frederick. AU - Han, Bumsoo. N1 - Funding Information: This research was supported by grants from the National Institute of Health / National Institute of Biomedical Imaging and Bioengineering , R01 EB008388 , and the National Science Foundation , CBET-1009465 . PY - 2011/8. Y1 - 2011/8. N2 - The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In ...
https://experts.illinois.edu/en/publications/effects-of-freezing-induced-cell-fluid-matrix-interactions-on-the
LABS Inc. | Tests | Directory | 366
Centrifuge and separate serum/plasma from cells and transfer to plastic vials; do not freeze whole blood. ,Frozen -20°C up to 4 weeks (with up to 5 freeze thaw cycles) ,Frozen -20°C up to 6 months (with up to 1 freeze thaw cycle) ...
http://labs-inc.org/tests/directory/366
Confused about the different principles to freeze & thaw cells and purified proteinConfused about the different principles to freeze & thaw cells and purified protein
When you freeze aqueous solutions slowly you form larger ice crystals. This would segregate water away from proteins and into the crystals of ice. As the amount of unfrozen water drops the protein becomes more and more concentrated and loses the water shell required to maintain its structure (remember the primary force for protein structure is the organisation of hydrophobic and hydrophilic residues). This quickly leads to protein denaturation and aggregation, and is one of the reasons why freeze thaw cycles are so damaging to proteins. Proteins are frozen quickly to produce the smallest ice crystals possible as quickly as possible, which minimises the disruption described above. Formulation of the protein with excipients such as polyols and carbohydrates can also help dramatically improve stability during freeze thaw by helping to lower the total amount of water bound up in ice crystals, and to take the place of water at the proteins surface. This can allow you to freeze proteins more slowly ...
http://www.bio.net/bionet/mm/methods/2009-July/104202.html
Freeze Thaw technology in handlings mAbs on another quality level | SingleUseSupportFreeze Thaw technology in handlings mAbs on another quality level | SingleUseSupport
Freeze & Thaw technology on another quality level | mAbs | Single-use technologies are highly flexible & scalable - learn more about our process!
https://www.susupport.com/freeze-thaw-technology-for-handling-mabs-on-a-higher-quality-level/
biochemistry - How do you store membrane proteins? - Biology Stack Exchangebiochemistry - How do you store membrane proteins? - Biology Stack Exchange
If i were to have a blind attempt at your question, it is never a good idea to freeze thaw any protein, not even cell lysates. Thats the whole point of glycerol use, which we use in our lab at 50% (v/v) to store bead bound proteins. Thats also why antibodies and restriction enzymes are stored in glycerol since freeze thawing antibodies dissolved in water often leads to their degradation, although you only tend to store stuff in glycerol, like restriction enzymes, if the concentration of the protein is very very high. One golden trick I use is aliquoting my samples and snap freeze them in liquid nitrogen and store in -80 freezer. Im curious as to what happens with your protein, does it degrade after a freeze thaw cycle? (Im aware that transmembrane proteins are notorious when it comes to storage/maintenance due to their awkward hydrophobic transmembrane region so its best to speak to an x-ray crystallography expert, if there is some one like that roaming around your research lab :), since they ...
https://biology.stackexchange.com/questions/7778/how-do-you-store-membrane-proteins
Team:Gaston Day/Notebook - 2010.igem.orgTeam:Gaston Day/Notebook - 2010.igem.org
1. Remove the 10X T4 DNA Ligase Reaction Buffer* from the freezer to thaw. You can remove the T4 DNA Ligase enzyme from the freezer at this point but leave the ligase in a cold box to keep it close to -20○ C. Thawing is fast if the buffer tube is immersed in room temperature water. Once thawed, agitate the 10X T4 DNA Ligase Reaction Buffer until all precipitate goes into solution. 2. Add 11ul of H2O to a 200ul PCR tube. 3. Add 2ul from each of the digest to the tube.** 4. Add 2ul of 10X T4 DNA Ligase Reaction Buffer to the tube. 5. Add 1ul of the T4 DNA Ligase to the tube. 6. The total volume in each tube should now be 20ul. Ensue the ligation is well mixed by flicking the tube. You can spin the tube in a microcentrifuge for a few seconds to collect the liquid in the bottom of the tube again. *Repeated freeze-thaw cycles of the buffer can degrade the ATP in the buffer thereby making the ligation reaction less efficient. It is wise to aliquot the buffer into 10ul aliquots prior to freezing ...
http://2010.igem.org/Team:Gaston_Day/Notebook
FAQs | Base AsiaFAQs | Base Asia
The shelf life for peptide in solution form is limited, especially for the peptides which contain Cysteine (C), Methionine (M), Tryptophan (W), Asparagine (N), Glutamine (Q) and N-Glutamic Acid (E) are prone to air oxidation. It is recommended to purge the air out of the vial and replace it with a blanket of nitrogen or argon. If storage in solution form is unavoidable, use sterile buffers around pH 5 to 6 and freeze aliquots for storage at -200oC to prolong the storage life of peptide in solution. Furthermore, avoid the use of frost-free freezer as there will be variations in temperature during the frequent defrosting cycle. Repeated freeze-thaw cycles can also damage the peptides. ...
https://base-asia.com/peptide-synthesis/support/faqs
ActoFactor™ Recombinant Human Erythropoietin (CHO) | Creative BioarrayActoFactor™ Recombinant Human Erythropoietin (CHO) | Creative Bioarray
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu EPO-alpha should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles ...
https://www.creative-bioarray.com/ActoFactor-Recombinant-Human-Erythropoietin-CHO-CSC-CTK0098-item-3424.htm
Anti-GFP Monoclonal Antibody (clone GF28R) 1 ml | COMMON EPITOPE TAGS | PROTEIN BIOLOGY | RESEARCH REAGENTS | BioFront...
Volume 1 ml (1 mg/ml) Description Anti-GFP (Green Fluorescent Protein) Tag (GF28R) Monoclonal Antibody Clonality | Host Species | Isotype GF28R | Mouse | IgG2b Immunogen GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates Specificity Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP Purification Protein A affinity chromatography from mouse ascites fluid Application Dot, ELISA, IP, IS, WB Sensitivity WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins Working Condition WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test Storage Store at -20 C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 C (add 0.05 NaN3) for several days to weeks Images Fig. 1:1000 (1 g/mL)
https://www.biofronttech.com/product/research-reagents-protein-biology-common-epitope-tags/anti-gfp-monoclonal-antibody-clone-gf28r-1-ml/1603118/
Protocol for Neural Progenitor Cell Expansion | Irvine ScientificProtocol for Neural Progenitor Cell Expansion | Irvine Scientific
Note : For neural progenitor cell expansion in monolayer, culture vessels have to be pre-coated with substrate for cell attachment. Either PRIME-XV Human Fibronectin or MatrIS F should be used as coating substrates. For consistent performance, usage of Poly-L-Ornithine is also recommended to be used in conjunction with PRIME-XV Human Fibronectin or PRIME-XV MatrIS F. Specific concentrations and types of substrates should be optimized for each end-user application. 1. Dissolve Poly-L-Ornithine in sterile PBS to make a 15 mg/mL stock (1000X). Aliquot and store at ≤-20˚C in a manual defrost freezer for up to 6 months. Avoid repeated freeze-thaw cycles.. 2. Dilute the 1000X Poly-L-Ornithine stock 1000-fold in sterile PBS to make a 15 µg/mL (1X) solution. Prepare fresh as needed.. 3. Add the (1X) Poly-L-Ornithine solution to culture vessel at a ratio of 0.15 mL/cm2. Incubate 3 hours to overnight at 37˚C and 5% CO2.. 4. Discard the Poly-L-Ornithine solution. Wash each vessel 3 times with equal ...
http://www.irvinesci.com/protocol-for-neural-progenitor-cell-expansion
BAF5346 - R&D Systems CLSTN1 antibody | AntibodypediaBAF5346 - R&D Systems CLSTN1 antibody | Antibodypedia
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution ...
https://antibodypedia.com/gene/2632/CLSTN1/antibody/698769/BAF5346
Native Mouse Glycated Hemoglobin A1c (HbA1c) Protein HbA1c-19M - Creative BioMart
Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles ...
https://www.creativebiomart.net/native-mouse-glycated-hemoglobin-a1c-hba1c-protein-470419.htm
Plus itPlus it
Methods: Analyses of HFABP were performed on an Advia 2400 (Siemens Healthcare Diagnostics). Imprecision, limit of detection (LOD), limit of blank (LOB), and linearity were assessed using standard methods. Stability was assessed at 4 °C, −20 °C, and with 3 repeated freeze-thaw cycles. Clinical diagnostic performance was assessed using chest pain in patients, with a final diagnosis according to the universal definition of myocardial infarction with cardiac troponin I (cTnI) measured on the Siemens Advia Centaur (cTnI Ultra method, 99th percentile 50 ng/L, 10% CV 30 ng/L). Ischemia was detected using sampling pre- and postangioplasty. ...
http://jalm.aaccjnls.org/content/1/6/702
Tianchi Small Liquid Nitrogen Tanks 3 Liter Ln2 Dewar Container Cryo Tank
TIANCHI Small Liquid Nitrogen Tanks 3 Liter LN2 Dewar Container Cryo Tank. Net Weight(KG):3.2. Gross Weight(KG):5.0. Static Power Dissipation(Kg/d):0.08. This Small Liquid Nitrogen Tanks 3 Liter is made of high-strength, special corrosion resistant aluminum alloy, light weight and easy to carry. Liquid nitrogen storage containers is well made, very good insulation, integrated molding, weld the small, solid and reliable, guarantee vacuum and prevent leakage. Liquid nitrogen container low loss, more than 5 years of vacuum quality.. Liquid nitrogen storage tank can be stored for a long biological specimens, semen, embryos, spawn, vaccines, skin, organ and other biological material. Products three guarantees service, for each customer to establish user files, and enjoy lifelong technical services. This LN2 Dewar Container is only suitable for the static storage of liquid nitrogen, not suitable for the transport of liquid nitrogen. This cryogenic container ships empty. It can be filled anywhere that ...
http://liquidnitrogendewars.com/tianchi-small-liquid-nitrogen-tanks-3-liter-ln2-dewar-container-cryo-tank.php
PA5-27732 - Invitrogen Antibodies PRKCG antibody | AntibodypediaPA5-27732 - Invitrogen Antibodies PRKCG antibody | Antibodypedia
PA5-27732 targets PKC gamma in WB applications and shows reactivity with Human samples. The PA5-27732 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 166 of Human PKC gamma. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles ...
https://www.antibodypedia.com/gene/32756/PRKCG/antibody/626618/PA5-27732
mylab | 重組蛋白質 / GNG11 Human; Guanine Nucleotide Binding Protein Gamma 11 Human Recombinant 20µg
Amino Acid SequenceMGSSHHHHHH SSGLVPRGSH MGSMPALHIE DLPEKEKLKM EVEQLRKEVK LQRQQVSKCS EEIKNYIEER SGEDPLVKGI PEDKNPFKEK GSC.DescriptionGNG11 Human Recombinant produced in E. coli is. a single polypeptide chain containing 93 amino acids (1-70) and having a molecular mass of 10.6kDa. GNG11 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.FormulationThe GNG11 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.Physical AppearanceSterile Filtered clear solution.PurityGreater than 90% as determined by SDS-PAGE.SourceEscherichia Coli.StabilityStore at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.SynonymsGNGT11, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11, GNG11.Linkhttp://www.prospecbio.com/GNG11_Human_10
https://www.mylab.com.tw/products/pro-1854-gng11-human-guanine-nucleotide-binding-protein-gamma-11-human-recombinant-20-g
White 2 Freezer Storage Boxes With 9 x 9 Grid from PGC ScientificsWhite 2 Freezer Storage Boxes With 9 x 9 Grid from PGC Scientifics
White 2 Freezer Storage Boxes With 9 x 9 Grid from PGC Scientifics,Fiberboard boxes are ideal for use in refrigerators, freezers, or liquid nitrogen. Conveniently sized to fit various storage racks. Four vibrant colors allow for color coding to project, user, lab, etc. Holes in the bottom allow liquid nitrogen drainage or promote cool air circulation. Supplied wit,biological,biology supply,biology supplies,biology product
http://www.bio-medicine.org/biology-products/White-2-22-Freezer-Storage-Boxes-With-9-x-9-Grid-from-PGC-Scientifics-4424-1/
Gullibles Travels: September 2012Gullibles Travels: September 2012
I live in Alaska. That means I am very well-acquainted with frost heaves, which are those nasty seasonal rises and depressions in what used to be smooth pavement. Caused by multiple freeze-thaw cycles in roadbeds prone to water retention, youre lucky if your vehicle doesnt bottom out on them, or worse, have the front bumper and rear bumper crash onto consecutive frost heaves, leaving it suspended in the air, or your vehicle bumper-less and your gas tank leaking ...
http://gullible-gulliblestravels.blogspot.com/2012/09/
ISISBio:Protocols/Sortase mediated ligation - OpenWetWareISISBio:Protocols/Sortase mediated ligation - OpenWetWare
These are the general reagents used for all ligation reactions. Discussion of the specific reagents will be in each individual protocol. Sortase: A Sortase A construct with the N-terminal membrane targeting sequence removed is readily purified in N-terminally His-tagged form. The pETMCSIII based vector pLLC064 is available for expression of this construct. The protein is easily overexpresed and purified using standard nickel affinity chromatography conditions. It should be noted that the protein migrates anomalously on SDS-PAGE gels and appears to run as a protein of ~26 kDa molecular weight (###). We have confirmed the correct molecular weight of Sortase A expressed from pLLC064 by MS. The protein is very stable. We find it convenient to store the protein in Sortase buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM CaCl2, pH 7.5). The protein appears stable to multiple freeze-thaw cycles. We have not as yet investigated the potential to freeze dry Sortase for storage although we hope to carry out this ...
https://openwetware.org/wiki/?title=ISISBio:Protocols/Sortase_mediated_ligation&oldid=208396
ASU Scholarship Showcase | ASU Digital RepositoryASU Scholarship Showcase | ASU Digital Repository
S-cysteinylated albumin and methionine-oxidized apolipoprotein A-I (apoA-I) have been posed as candidate markers of diseases associated with oxidative stress. Here, a dilute-and-shoot form of LC-electrospray ionization-MS requiring half a microliter of blood plasma was employed to simultaneously quantify the relative abundance of these oxidized proteoforms in samples stored at −80 °C, −20 °C, and room temperature and exposed to multiple freeze-thaw cycles and other adverse conditions in order to assess the possibility that protein oxidation may occur as a result of poor sample storage or handling. Samples from a healthy donor and a participant with poorly controlled type 2 diabetes .... ...
https://repository.asu.edu/collections/160?cont=Borges%2C+Chad
Glory Bio Science Co. LTDGlory Bio Science Co. LTD
Do not mix reagents with those from other lots.. Reactivity : Bovine. Method type : Sandwich ELISA. Minimum Detection Limit :. Detection Range :. Application : ELISA. Purpose : For the quantitative determination of target substances concentrations.. Research Area : Immunology-,Innate Immunity-,Complement-,Alternative Pathway, Immunology-,Innate Immunity-,Complement-,Other. Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates. Plate : Pre-coated,Strips (12 x 8). Restrictions : For Research Use only. Storage : 2 °C - 8 °C. Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. Expiry Date : 12 months. Size : 96T. Uniprot No. :. Abbreviation :. Availability : 3-5 working days. Target Details :. Precision : Intra-assay Precision (Precision within an assay) CV%,15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays) CV%,15% Three samples of ...
https://www.glorybios.com/product-center/sumo-activating-enzyme-subunit-1-sae1-bovine-elisa-kit/
Endpoint PCR Ready Mixes and Polymerases :: PCRBIO dNTP Mix - PCR Biosystems - Simplifying ResearchEndpoint PCR Ready Mixes and Polymerases :: PCRBIO dNTP Mix - PCR Biosystems - Simplifying Research
Features More than purity Stable after multiple freeze-thaw cycles dNTPs triphospate form after weeks room temperature Perfect standard real-time high-fidelity 1-Step long range PCRBIO dNTP ultra-pure stable suitable variety applications PCRBIO dNTP been tested many molecular biology applications including standard fast 1-Step high-fidelity real-time long-range LAMP sequencing
http://www.pcrbio.com/end-point-pcr-reagents/pcrbio-dntp-mix.html
Rat Endothelial nitric oxide synthase 3,eNOS-3 ELISA Kit - Glory Bio Science Co. LTDRat Endothelial nitric oxide synthase 3,eNOS-3 ELISA Kit - Glory Bio Science Co. LTD
Do not mix reagents with those from other lots.. Reactivity : Rat. Method type : Sandwich ELISA. Minimum Detection Limit : Detection Range : Application : ELISA. Purpose : For the quantitative determination of target substances concentrations.. Research Area : Immunology->Innate Immunity->Complement->Alternative Pathway, Immunology->Innate Immunity->Complement->Other. Sample Type : serum, plasma, Urine, tissue samples, cell culture supernates. Plate : Pre-coated,Strips (12 x 8). Restrictions : For Research Use only. Storage : 2 °C - 8 °C. Storage Comment : Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. Expiry Date : 12 months. Size : 96T. Uniprot No. : Abbreviation : Availability : 3-5 working days. Target Details : Precision : Intra-assay Precision (Precision within an assay) CV ...
https://www.glorybios.com/product-center/rat-endothelial-nitric-oxide-synthase-3enos-3-elisa-kit/
Patent US7128081 - Peltier based freeze-thaw valves and method of use - Google PatentsuchePatent US7128081 - Peltier based freeze-thaw valves and method of use - Google Patentsuche
A freeze-thaw valve is provided using a Peltier heat pump where the thermal short-circuit path between a cooled thermal mass and a heated thermal mass is reduced or absent and the valve state transition time is minimized. The freeze-thaw valve comprises a Peltier heat pump mounted to a heat exchange surface that comprises a cross-drilled copper water jacket or manifold. The Peltier heat pump is operated to maintain a cooled thermal mass at a substantially constant low temperature. A resistance heating element is used to produce a heated thermal mass. The freeze-thaw segment of a fluid conduit is commutated to contact either the heated or the cooled thermal mass to thaw and therefore open the valve or cool and thus close the valve. The operation of the Peltier heat pump at a constant temperature avoids problems inherent in the use of a Peltier heat pump to both heat and cool a freeze thaw segment.
http://www.google.de/patents/US7128081?hl=de
Difference between revisions of Glycerol - OpenWetWareDifference between revisions of Glycerol - OpenWetWare
Glycerol is also used to decrease the freezing point of protein solution which are easily degraded by repeated freeze/thaw cycles. A 50% (w/v) glycerol/water solution freezes at -25ºC and can thus be stored in a -20ºC freezer. Note that glycerol with 1.26g/cm3 is much denser than water and, therefore, a 50% (v/v) solution will be very different from a 50% (w/v) solution. For example, restriction enzymes are typically stored in a glycerol/water solution. ...
https://openwetware.org/wiki/?title=Glycerol&diff=prev&oldid=366093
VEGF-165, mouse, recombinant (E. coli) | PromoCellVEGF-165, mouse, recombinant (E. coli) | PromoCell
The lyophilized rMu VEGF, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu VEGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.10% endotoxin-free HSA or BSA; e.g. Cat.No. C-69500A). Avoid repeated freeze / thaw cycles ...
https://promocell.com/product/vegf-165-mouse-recombinant-e-coli/
ActoFactor™ Recombinant Human Interleukin 12 (HEK) | Creative BioarrayActoFactor™ Recombinant Human Interleukin 12 (HEK) | Creative Bioarray
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-12 should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles ...
https://www.creative-bioarray.com/ActoFactor-Recombinant-Human-Interleukin-12-HEK-CSC-CTK0247-item-3573.htm
Global Ultra-low Temperature Freezer Storage Tank Market Size and Forecast to 2021Global Ultra-low Temperature Freezer Storage Tank Market Size and Forecast to 2021
Ultra-low Temperature Freezer Storage Tank Report by Material, Application, and Geography Global Forecast to 2021 is a professional and comprehensive research report on the worlds major regional market conditions,
http://www.aarkstore.com/industrial-goods-machinery/630616/global-ultra-low-temperature-freezer-storage-tank-market-size-and-forecast-to-2021
Global Ultra-low Temperature Freezer Storage Tank Market Report and Forecast to 2021Global Ultra-low Temperature Freezer Storage Tank Market Report and Forecast to 2021
Ultra-low Temperature Freezer Storage Tank Report by Material, Application, and Geography Global Forecast to 2021 is a professional and comprehensive research report on the worlds major regional market conditions,
http://www.aarkstore.com/industrial-goods-machinery/630615/global-ultra-low-temperature-freezer-storage-tank-market-report-and-forecast-to-2021
Chondrex: new marker of joint disease | Clinical ChemistryChondrex: new marker of joint disease | Clinical Chemistry
Chondrex, a major secretory protein of human chondrocytes and synovial cells, has been previously shown to be increased in the synovial fluid and serum of individuals with joint and cartilage disease (3)(6). We have developed a simple, fast, two-site, sandwich-type ELISA that uses a streptavidin-coated microplate well, a biotinylated-Fab monoclonal capture antibody, and an alkaline phosphatase-labeled polyclonal detection antibody. The assay involves three 1-h incubation steps, is carried out at room temperature, and does not require sample pretreatment.. The Chondrex assay has good within-run and between-run precision, good linearity upon dilution of samples, and good analytical recovery. Chondrex concentrations in serum samples are stable at 4 °C for as long as 9 days and as many as 6 freeze/thaw cycles. The measurement of chondrex in serum is not affected by abnormally high concentrations of common serum components such as bilirubin, hemoglobin, triglycerides, and total protein. In addition, ...
http://clinchem.aaccjnls.org/content/44/3/509.long
SULIS: Sustainable Urban Landscape Information Series : University of Minnesota ExtensionSULIS: Sustainable Urban Landscape Information Series : University of Minnesota Extension
Some deciduous shrubs can benefit from extra care for winter months. While most deciduous shrubs are not susceptible to sun scald like deciduous trees are, those with larger central stems or trunks exposed to full sun may benefit from wrapping. Newly planted shrubs may also benefit from extra mulch over the root zone to prevent damage from the freeze/thaw cycles of late winter, which can damage roots. Salt used to melt ice on pavement can damage shrubs in two ways. Shrubs planted near roadways often suffer from salt spray damage, resulting in bud death and twig dieback. New growth then occurs mainly near the branch bases, creating clusters of twigs called witches brooms. Salt can also get into soil in several ways, including being sprayed by traffic, plowed off the road, or by draining with melted snow and ice onto soil surfaces. Salt in the soil can kill roots directly or can prevent them from taking up water, causing drought injury. Often, injury cased by salt exposure does not show up for ...
http://www.extension.umn.edu/garden/landscaping/maint/shrubs.html
Polyclonal antibody for rat cytochrome P450 enzyme CYP2D1 - Quicktech
Rabbit anti-rat cytochrome P450 enzyme CYP2D1 (polyclonal antibody). O-demethylation of xenobiotics, CYP2D6 is involved in the metabolism and elimination of approximately 25% of clinically used drugs in humans1. Primarily expressed in the liver and areas of the CNS. CYP2D1 and CYP2D4 have a similar function in rats2.. CODE NUMBER: RK05.. QUANTITY: 0.1 mL.. SPECIFICITY: Reacts with rat cytochrome P450 enzyme CYP2D1 in hepatic microsomal fraction.. IMMUNOGEN: Synthetic peptide.. SUGGESTED APPLICATIONS: Western blot, immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user.. SPECIES REACTIVITY: Rat.. FORMAT: Rabbit antiserum.. PRESENTATION: Liquid. No preservatives.. STORAGE/HANDLING: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.. For research use only; not for use in diagnostic procedures. Not for human or animal consumption.. CYP2D1 is also known as CYPIID1, Cyp2d-1, Cyp2d9 and ...
http://quicktech.imperialinnovations.co.uk/i/Antibodies/CYP2D1.html
Effect of freeze-thaw cycles on quality of Pacific white shrimp « Global Aquaculture AdvocateEffect of freeze-thaw cycles on quality of Pacific white shrimp « Global Aquaculture Advocate
Study evaluates the effects of freeze-thaw cycles on moisture migration, protein degradation, microstructure and quality of Pacific white shrimp.
https://www.aquaculturealliance.org/advocate/effect-of-freeze-thaw-cycles-on-quality-of-pacific-white-shrimp/
Influence of different storage conditions and anticoagulants on the measurement of total and acylated ghrelin in dogs: a...
The objective of this study was to evaluate and compare different sample treatments and storage conditions in order to determine optimal conditions for total and acylated canine ghrelin measurement using ELISA assays. Under the conditions assessed in this study, total ghrelin concentration was stable when stored at room temperature or under cooled conditions (4°C) for approximately six hours regardless of the type of anticoagulant used. When samples were stored at under −20°C, three freeze/thaw cycles during storage did not alter total ghrelin concentration if serum or plasma treated with EDTA or with EDTA-aprotinin is used. When samples were stored at under −80°C, no significant differences were observed after three freeze/thaw cycles in total ghrelin concentrations in serum or plasma treated with EDTA, EDTA-aprotinin or heparin. While acylated ghrelin showed to be very instable, its analysis should be performed within an hour of sample collection or stored at −80°C preferably in ...
http://veterinaryrecord.bmj.com/content/172/11/289
Free polyunsaturated fatty acids cause taste deterioration of salmon during frozen storage<...Free polyunsaturated fatty acids cause taste deterioration of salmon during frozen storage<...
TY - JOUR. T1 - Free polyunsaturated fatty acids cause taste deterioration of salmon during frozen storage. AU - Refsgaard, Hanne. AU - Brockhoff, P.M.B.. AU - Jensen, Benny. PY - 2000. Y1 - 2000. N2 - Increased intensity of train oil taste, bitterness, and metal taste are the most pronounced sensory changes during frozen storage of salmon (Refsgaard, H. H. F.; Brockhoff, P. B.; Jensen, B. Sensory and Chemical Changes in Farmed Atlantic Salmon (Salmo salar) during Frozen Storage. J. Agric. Food Chem. 1998a, 46, 3473-3479). Addition of each of the unsaturated fatty acids: palmitoleic acid (16:1, n - 7), linoleic acid (C18:2, it - 6), eicosapentaenoic acid (EPA; C20:5, it - 3) and docosahexaenoic acid (DHA; C22:6, n. - 3) to fresh minced salmon changed the sensory perception and increased the intensity of train oil taste, bitterness, and metal taste. The added level of each fatty acid (similar to 1 mg/g salmon meat) was equivalent to the concentration of the fatty acids determined in salmon stored ...
https://orbit.dtu.dk/en/publications/free-polyunsaturated-fatty-acids-cause-taste-deterioration-of-sal
Time of flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project -...
Fingerprint Dive into the research topics of Time of flight mass spectrometry of DNA laser-ablated from frozen aqueous solutions: applications to the Human Genome Project. Together they form a unique fingerprint. ...
https://asu.pure.elsevier.com/en/publications/time-of-flight-mass-spectrometry-of-dna-laser-ablated-from-frozen/fingerprints/
Disease Prone: Zombies Pt 2 (or how I distracted my students)Disease Prone: Zombies Pt 2 (or how I distracted my students)
The winter hibernation in northern North America is very important because of how long and extreme it can be. To up the challenge the wood frog hibernates in the leaf litter and consequently freezes solid during winter. By solid I mean properly solid. The frogs can survive multiple freeze/thaw events as long as less than 65% of their water remains unfrozen. Freeze/thaw stops my experiments on bacteria from working but apparently these frogs are just fine. To survive the big freeze the frogs accumulate urea in the tissues and build enormous glycogen stores which breaks into glucose upon ice formation in the tissues. The soluble urea and glucose prevent ice formation and save the frogs. This phenomenal life cycle enables easier and early access to the natal ponds where eggs are laid and tadpoles develop and must leave before the thawing dries up the pools. So the question was posed, is a wood frog alive during winter hibernation? Technically its dead, no heartbeat, no metabolism and yet it ...
http://diseaseprone.fieldofscience.com/2011/05/zombies-pt-2-or-how-i-distracted-my.html?showComment=1304833167944
Human Apolipoprotein C2 , apo-C2 ELISA Kit
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles ...
http://www.egfie.com/product/CSB-E14174h
Urinary p75ECD: A prognostic, disease progression, and pharmacodynamic biomarker in ALS<...
TY - JOUR. T1 - Urinary p75ECD: A prognostic, disease progression, and pharmacodynamic biomarker in ALS. AU - Shepheard, Stephanie. AU - Wuu, Joanne. AU - Cardoso, Michell. AU - Wiklendt, Lukasz. AU - Dinning, Philip. AU - Chataway, Timothy. AU - Schultz, David. AU - Benatar, Michael. AU - Rogers, Mary-Louise. PY - 2017. Y1 - 2017. N2 - Objective: To evaluate urinary neurotrophin receptor p75 extracellular domain (p75 ECD) levels as disease progression and prognostic biomarkers in amyotrophic lateral sclerosis (ALS). Methods: The population in this study comprised 45 healthy controls and 54 people with ALS, 31 of whom were sampled longitudinally. Urinary p75 ECD was measured using an enzyme-linked immunoassay and validation included intra-assay and inter-assay coefficients of variation, effect of circadian rhythm, and stability over time at room temperature, 4°C, and repeated freeze-thaw cycles. Longitudinal changes in urinary p75 ECD were examined by mixed model analysis, and the prognostic ...
https://researchnow.flinders.edu.au/en/publications/urinary-p75ecd-a-prognostic-disease-progression-and-pharmacodynam
Beginning of exocytosis captured by rapid-freezing of Limulus amebocytes. - Semantic ScholarBeginning of exocytosis captured by rapid-freezing of Limulus amebocytes. - Semantic Scholar
Structural changes underlying exocytosis evoked by the application of endotoxin to Limulus amebocytes were studied at the level of detail afforded by freeze-fracture and freeze-substitution techniques combined with the time resolution of direct rapid-freezing. The results with amebocytes prepared in this manner differed from those with other secretory cells prepared by conventional means. Exocytosis begins within seconds of endotoxin treatment when the plasmalemma invaginates to form pedestallike appositions with peripheral secretory granules. The juxtaposed membranes at these pedestal appositions form several punctate pentalaminar contacts, but examination of freeze-fractured pedestals failed to reveal any corresponding changes in the intramembrane particle distribution. Small secretory granule openings or pores, which are very infrequent, appear within the first 5 s after endotoxin treatment. These pores rapidly widen and this widening is immediately followed by the sequential dissolution of the
https://www.semanticscholar.org/paper/Beginning-of-exocytosis-captured-by-rapid-freezing-Ornberg-Reese/0e39e110432194f8a2c4fea64dcfbfd37bbb6f10
Effects of freezing-induced cell-fluid-matrix interactions on cells and extracellular matrix of engineered tissues<...
TY - GEN. T1 - Effects of freezing-induced cell-fluid-matrix interactions on cells and extracellular matrix of engineered tissues. AU - Teo, Ka Yaw. AU - Dutton, J. Craig. AU - Grinnell, Frederick. AU - Han, Bumsoo. PY - 2011/12/1. Y1 - 2011/12/1. UR - http://www.scopus.com/inward/record.url?scp=84881235080&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84881235080&partnerID=8YFLogxK. U2 - 10.1115/SBC2011-53407. DO - 10.1115/SBC2011-53407. M3 - Conference contribution. AN - SCOPUS:84881235080. SN - 9780791854587. T3 - ASME 2011 Summer Bioengineering Conference, SBC 2011. SP - 133. EP - 134. BT - ASME 2011 Summer Bioengineering Conference, SBC 2011. T2 - ASME 2011 Summer Bioengineering Conference, SBC 2011. Y2 - 22 June 2011 through 25 June 2011. ER - ...
https://utsouthwestern.pure.elsevier.com/en/publications/effects-of-freezing-induced-cell-fluid-matrix-interactions-on-cel
NTNU Open: Cold-hearted bats: uncoupling of heart rate and metabolism during torpor at sub-zero temperaturesNTNU Open: Cold-hearted bats: uncoupling of heart rate and metabolism during torpor at sub-zero temperatures
Many hibernating animals thermoregulate during torpor and defend their body temperature (Tb) near 0°C by an increase in metabolic rate. Above a critical temperature (Tcrit), animals usually thermoconform. We investigated the physiological responses above and below Tcrit for a small tree-dwelling bat (Chalinolobus gouldii, ∼14 g) that is often exposed to sub-zero temperatures during winter. Through simultaneous measurement of heart rate (fH) and oxygen consumption (V̇O2), we show that the relationship between oxygen transport and cardiac function is substantially altered in thermoregulating torpid bats between 1 and −2°C, compared with thermoconforming torpid bats at mild ambient temperatures (Ta 5-20°C). Tcrit for this species was at a Ta of 0.7±0.4°C, with a corresponding Tb of 1.8±1.2°C. Below Tcrit, animals began to thermoregulate, as indicated by a considerable but disproportionate increase in both fH and V̇O2. The maximum increase in fH was only 4-fold greater than the average ...
https://ntnuopen.ntnu.no/ntnu-xmlui/handle/11250/2592642
Storage temperaturesStorage temperatures
Jim Hartley ,jhartley at access2.digex.net, writes: ,for differing suspensions (i.e. plasmids). , ^^^^^^^^ .... : Do you mean that repeated freezing and thawing can do damage to : my plasmid DNA preps? , A few years back a guy here took a plasmid and cycled it between 37 C and , dry ice, my memory is 30 times, and took out samples during the process. , By gel analysis the degree of supercoiling was entirely unchanged by all , this freezing and thawing. I know there is an ancient bias that freezing , is bad for plasmids, but for DNA less than lambda size I have never seen , any data demonstrating this. I fear microbial growth and slow enzymatic , degradation much more than freeze/thaw cycles, so all my DNA is tucked away , in a frost free -20 freezer. , , Jim Hartley , Life Technologies, Inc. Thanks for the response Jim. I did know about the old BRL study, and I was trying to see if Ted meant all samples in the freezer, including plasmid DNA. I did e-mail him about the study done by BRL that ...
http://www.bio.net/bionet/mm/methods/1995-June/030347.html
Freezer Storage Boxes and Work Station Racks, Labnet R8300 Polypropylene, for 0.5 mL and 1.5/2.0 mL Micro-centrifuge tubesFreezer Storage Boxes and Work Station Racks, Labnet R8300 Polypropylene, for 0.5 mL and 1.5/2.0 mL Micro-centrifuge tubes
Labnet R8300 Polypropylene freezer storage boxes withstand autoclaving and freezing to -80ºC. The 3-point hinged lid maintains its integrity during repeated use. A positive catch keeps the lid securely fastened. Alpha-numeric indexing is printed inside the boxes for easy sample ID, and the clear lid allows visibility even when the it is closed. Boxes are available in a variety of colors for coding samples and a solid black box is available for light sensitive samples. The freezer boxes measure 142 x 142 x 55 mm and hold one hundred 1.5/2.0 mL tubes, cryovials or chromatography vials. The clear plastic work station racks come in two versions, to hold 40 x 0.5 mL microtubes or 40 × 1.5/2.0 mL microtubes. Robust construction makes sturdy racks for every day usage. They are autoclavable and the open bottom allows their use in ice baths.
http://rosesci.com/Products/products.php?search_go=Cryoboxes
Relating Freeze-induced Electrolyte Leakage Measurements to Lethal Temperature in Red Raspberry in: Journal of the American...
Seasonal cold hardiness of red raspberry (Rubus idaeus L.) canes was measured by freeze-induced electrolyte leakage test and visual rating of injury. Leakage data were transformed to percentage-adjusted injury values and related to lethal temperature by graphical interpolation and by the midpoint (T50) and inflection point (Tmax) estimates derived from three sigmoid (the logistic, Richards, and Gompertz) functions. Tmax estimates produced by Richards and Gompertz functions were corrected further using two different procedures. The 10 leakage-based hardiness indices, thus derived, were compared to lethal-temperature estimates based on visual rating. Graphical interpolation and Tmax of the logistic or T50 of the Gompertz function yielded lethal-temperature estimates closest to those obtained visually. Also, Tmax values of the Gompertz function were well correlated with visual hardiness indices. The Richards function yielded hardiness estimates deviating largely from visual rating. In addition, the ...
https://journals.ashs.org/jashs/view/journals/jashs/125/4/article-p429.xml?rskey=BKFrCz
A scientist lowers biological samples into a liquid nitrogen storage... News Photo - Getty ImagesA scientist lowers biological samples into a liquid nitrogen storage... News Photo - Getty Images
A scientist lowers biological samples into a liquid nitrogen storage tank at the Cancer Research UK Cambridge Institute on December 9, 2014 in Cambridge, England. Healthy and cancerous biological... Get premium, high resolution news photos at Getty Images
https://www.gettyimages.com/detail/news-photo/scientist-lowers-biological-samples-into-a-liquid-nitrogen-news-photo/463580650
Crumb rubber used in concrete to provide freeze-thaw protection (optimal particle size)<...
TY - JOUR. T1 - Crumb rubber used in concrete to provide freeze-thaw protection (optimal particle size). AU - Richardson, Alan. AU - Coventry, Kathryn. AU - Edmondson, Vikki. AU - Dias, Eli. PY - 2016/1/20. Y1 - 2016/1/20. N2 - This research has examined the optimum particle size of crumb rubber, used as an additive to concrete that would provide maximum freeze-thaw protection whilst minimising the compressive strength loss. The crumb rubber as used in the paper was divided into five batches, with increasing particle size, graded in increments of 0.5mm, from 2.5mm. The primary properties of the concrete investigated were; air content, freeze-thaw durability and compressive strength. These were tested using standard test methods. The range of tests used were conclusive in that the ,0.5mm crumb rubber particle size, provided the greater degree of air entrainment. The freeze-thaw cycle results suggested that crumb rubber provided freeze-thaw protection, as the plain concrete deteriorated compared ...
https://researchportal.northumbria.ac.uk/en/publications/crumb-rubber-used-in-concrete-to-provide-freezethaw-protection-op
Liquid Nitrogen Storage Dewar Roller Base | Brymill Cryogenic SystemsLiquid Nitrogen Storage Dewar Roller Base | Brymill Cryogenic Systems
Maneuver your liquid nitrogen storage dewar safely and easily with a roller base from by Brymill Cryogenic Systems, a provider of cryogenic equipment.
https://www.brymill.com/products/ln2-storage-equipment/dewar-roller-base
Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples | Microbiome | Full...Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples | Microbiome | Full...
In this study, we developed and validated a sample preparation protocol for unbiased amplification and high-throughput metagenomic sequencing of viruses in routine diagnostic use. In an unbiased metagenomic approach, prior knowledge of the virus sequence is not required. In principle, it can therefore detect any virus. However, sample processing needs to be optimized for recovery and amplification of viral genomes that might be present only in very small amounts in clinical samples. We optimized such a protocol using plasma samples spiked with different classes of viruses.. First, we tested filtration, extraction, and nuclease digestion procedures in order to find optimal conditions for virus enrichment and reduction of unwanted, non-viral reads. Filtration proved to be indispensable, as the number of virus reads after sequencing significantly increased. Additionally, sample pre-processing including a freeze thaw cycle showed the best enrichment (Fig. 1), and for best integration into the daily ...
https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-017-0317-z
Polyclonal antibody for rat Cytochrome P450 enzyme CYP1A1 - Quicktech
Rabbit anti-rat cytochrome P450 enzyme CYP1A1 (polyclonal antibody). Involved in metabolism of xenobiotics, drugs and polyunsaturated fatty acids. Implicated in metabolism of some polycyclic hydrocarbons to carcinogenic intermediates. CODE NUMBER: R2U.. QUANTITY: 0.1 mL.. SPECIFICITY: Reacts with rat cytochrome P450 enzyme CYP1A1 in hepatic microsomal fraction. No cross-reactivity with other cytochrome P450 enzymes including CYP1A2.. IMMUNOGEN: Synthetic peptide.. SUGGESTED APPLICATIONS: Western blot, immunohistochemistry on frozen and formaldehyde treated sections. Optimal working dilutions must be determined by end user.. SPECIES REACTIVITY: Rat.. FORMAT: Rabbit antiserum.. PRESENTATION: Liquid. No preservatives.. STORAGE/HANDLING: Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.. For research use only; not for use in diagnostic procedures. Not for human or animal consumption.. To purchase this product, please email ...
http://quicktech.imperialinnovations.co.uk/i/Antibodies/rabbit-anti-rat.html
SDman </span...SDman </span...
In regards to DEF storage/use in seasonal equipment for us in the northern climate, this is something that can/will happen due to repeated freeze/thaw cycles and long periods being frozen…the liquid urea can separate from the water, leading to whats referred to as crystallized DEF. The stuff looks like chunks of dry fertilizer or golf balls, take your pick. If it sits long enough, eventually it will blend together again for a DEF solution that shouldnt give you any problems; however, if you need to use it sooner rather than later, you will have to get this stuff out of your DEF tank or else you will have problems. We run into this on Steigers every spring when they are waking up from their long winters nap; combines dont have this problem as they get to sit for another 3-4 months before waking up for another season. CNH recommends draining the DEF tank for long periods of inactivity and flushing them with water. Trust me, hardly anybody does it. If nothing else, I recommend running the ...
https://www.redpowermagazine.com/forums/profile/1231-sdman/
Utah Valley Gardens: November 2008Utah Valley Gardens: November 2008
Cyclamen can grow in zones 5/6 as long as a few rules are observed. Hederifolium puts out roots from the top and sides of the tuber, unlike Cyclamen coum and the like which have roots on the bottom surface only, so they need to be planted with the tops of the tubers a couple inches deep (place soil up to the top and then cover the top with a couple inches of coarse grit rather than garden soil). The best locations are on a north facing slope under a tree, or on the north side of the house that doesnt get any direct sun in the winter. Sunlight makes the normal freeze/thaw cycles even worse. Note: if you see ants on these plants dont worry, this is a good thing because they disperse the seed ...
http://utahvalleygardens.blogspot.com/2008_11_01_archive.html
House-Anderson Mt Alberta - Uisdean Hawthorn
After last years visit to Canada we questioned our decision to bail off the House-Anderson route on the North Face of Mt Alberta due to bad conditions, we questioned if we should try at a different time of year, and questioned if the route was too big, hard and committing for us. The lure of unfinished business on such an iconic mountain was too big for us not to take the risk and think about going back to Canada for round two this September. When Nick Sharpe offered us a place to stay and use of his jeep all for a few beers it was an easy decision-what a legend! After getting over jet lag and trying to get information on conditions over the first week of our trip a 7 day weather window appeared on the forecast. This was perfect as it allowed us to let the recent snow go through a freeze thaw cycle, before we headed on the long walk up and over Woolly Shoulder.. On Wednesday the 14th we stashed gear, rigged the abseil station for the rappels down to the glacier below the north face and made ...
https://www.uisdeanhawthorn.com/Photo-Journal/House-Anderson-Mt-Alberta/i-WVH2dkX/
Changes in Membrane Phospholipids as a Mechanistic Explanation for Decreased Freeze Tolerance in Earthworms Exposed to...Changes in Membrane Phospholipids as a Mechanistic Explanation for Decreased Freeze Tolerance in Earthworms Exposed to...
article{d127567d-2071-4748-b996-09807a0c4a66, abstract = {At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant negative effect on the polyunsaturated PLFA, linoleic acid (18:2 omega 6,9), which has previously been reported to correlate positively (R-2 = 0.92) with freeze tolerance in D. octaedra. This supports our ...
https://lup.lub.lu.se/search/publication/1461698
Liquid Nitrogen: Characteristics, Uses and Safety Concerns - Nova Science PublishersLiquid Nitrogen: Characteristics, Uses and Safety Concerns - Nova Science Publishers
BISAC: SCI013000. In petroleum engineering, liquid nitrogen can be used to aid in fracturing the formation rock because of its extremely cryogenic temperature. With the development of new jet technology in recent years, liquid nitrogen is considered as a promising jet medium to exploit oil and gas. The first chapter of this book provides an impact pressure and parametric sensitivity analysis of the numerical simulation of liquid nitrogen.. In the second chapter, the uses of liquid nitrogen in a completely different sphere are explored, particularly its use for the storage of human cells and tissues for transplantation. Finally, liquid nitrogen is one of the cryogenic fluids most used for cryopreservation of animal germoplasm; low temperature preservation of oocytes, sperm and embryos is a fundamental cornerstone of assisted reproductive technologies. The last chapter examines the mathematical prediction of cooling rates during cryopreservation of reproductive cells in liquid nitrogen ...
https://novapublishers.com/shop/liquid-nitrogen-characteristics-uses-and-safety-concerns/
Transgenic Rat or Mouse Spinal Cord (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells - Science...Transgenic Rat or Mouse Spinal Cord (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells - Science...
Transgenic Rat or Mouse Spinal Cord (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells - Science Exchange Lets You Compare Quotes From Leading Service Providers.
https://www.scienceexchange.com/marketplace/transgenic-rat-or-mouse-spinal-cord-embryonic-postnatal-adult-fresh-tissue-suspended-in-media-not-frozen-cells
Transgenic Rat or Mouse Sciatic Nerve (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells -...Transgenic Rat or Mouse Sciatic Nerve (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells -...
Transgenic Rat or Mouse Sciatic Nerve (Embryonic; Postnatal; Adult): Fresh tissue suspended in media, not frozen cells - Science Exchange Lets You Compare Quotes From Leading Service Providers.
https://www.scienceexchange.com/marketplace/transgenic-rat-or-mouse-sciatic-nerve-embryonic-postnatal-adult-fresh-tissue-suspended-in-media-not-frozen-cells
Oogenesis at subzero temperatures: a comparative study of the oocyte morphology in nine species of Notothenioides | Archivio...
Oogenesis was examined in nine species of Antarctic fish to verify the existence of morphological peculiarities. The analyses were carried out on specimens belonging to three different families of Notothenioids (Nototheniidae, Channichthyidae and Bathydraconidae), all captured in the Ross Sea, in front of the Italian Station of Terra Nova Bay. Following dissection, the ovaries were processed and examined at the light and electron microscopes to determine the oocyte gross and fine morphology. The attention, in particular, was focused on the presence of cytoplasmic round bodies and on the organization of the cortical alveoli and the vitelline envelope. Results reveal significant specie-specific differences that could be partly correlated to the phylogenetic radiation but not to the peculiar environmental conditions being essentially comparable to those observed among temperate species.. ...
https://ricerca.uniparthenope.it/handle/11367/53383
Unfolding the Secrets of Ice Formation to Better Understand Climate | | SBU NewsUnfolding the Secrets of Ice Formation to Better Understand Climate | | SBU News
STONY BROOK, NY, August 7, 2020 - Ice crystal formation plays a crucial role in precipitation formation and alters the radiative properties of clouds, thereby affecting Earths climate system. In recent years there has been tremendous progress in understanding how liquid-phase droplets are formed (nucleation), yet the nucleation of ice crystals has remained elusive. Thats why Daniel Knopf, PhD, Professor in the School of Marine and Atmospheric Sciences (SoMAS) at Stony Brook University, is studying the nucleation of ice crystals with the goal to improve current cloud and climate modeling approaches. The research is supported by a three-year $710,000 grant from the Department of Energy that starts September 15, 2020.. Ice formation is considered one of the remaining grand challenges in the atmospheric sciences, says Professor Knopf. The objective of the project is to gain a predictive understanding of the chain that leads from aerosols to ice-nucleating particles to ice crystal number ...
https://news.stonybrook.edu/newsroom/unfolding-the-secrets-of-ice-formation-to-better-understand-climate/
Withdrawal Archives - Cryoccessories: Liquid Nitrogen Dewars for RentWithdrawal Archives - Cryoccessories: Liquid Nitrogen Dewars for Rent
Need a liquid nitrogen dewar to rent? Do you want to make liquid nitrogen ice cream at home? Do you like experimenting with liquid nitrogen? Do you need a liquid nitrogen dewar or cryogun for your doctors office? If so, you came to the right place. We sell and rent liquid nitrogen dewars for cooking, experimenting and also medical use ...
https://cryoccessories.com/product-category/medical-rentals/withdrawal-systems-medical-rentals/
liquid nitrogen - Sichuan liquid nitrogen Manufacturer
liquid nitrogen Manufacturers & liquid nitrogen Suppliers Directory - choose liquid nitrogen from 2148 Sichuan liquid nitrogen Super Suppliers of China.
http://www.fazendomedia.com/sale-liquid-nitrogen/province-32000000.html
liquid nitrogen - Jiangsu liquid nitrogen Manufacturer
liquid nitrogen Manufacturers & liquid nitrogen Suppliers Directory - choose liquid nitrogen from 2196 Jiangsu liquid nitrogen Super Suppliers of China.
http://www.fazendomedia.com/sale-liquid-nitrogen/province-19000000.html
Human CALY Protein Lysate 20ug - Innovative ResearchHuman CALY Protein Lysate 20ug - Innovative Research
Human CALY Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. This is a Recombinant Protein Lysate produced in HEK293T cells. This protein lysate can be reconsituted using SDS Sample Buffer. Once reconsituted, aliquot and store any remaining material at -20C to avoid repeated freeze-thaw
https://www.innov-research.com/products/human-caly-protein-lysate
Robert Hnasko : USDA ARSRobert Hnasko : USDA ARS
Hnasko, R.M., Lin, A.V., Stanker, L.H., Bala, K., McGarvey, J.A. 2016. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional renilla-prion fusion protein from bacteria. In: Micic, M., editor. Sample Preparation Techniques for Soil, Plant, and Animal Samples. New York, NY: Humana Press. p. 389-399 ...
https://www.ars.usda.gov/people-locations/person/?person-id=39021&pf=1
Freezing Point Depression - GCSE Science - Marked by Teachers.comFreezing Point Depression - GCSE Science - Marked by Teachers.com
Introduction. Level 1 Pharmacy Physicochemical Principles of Pharmacy 315PMY105 Name: Catherine Magee Student number: 13324063 Practical: 3 - Freezing Point Depression Aims � Demonstration of the effect of solutes used in aqueous formulations on the freezing point of water. � Application of the knowledge of the depression of freezing point to the determination of the molecular weight of an unknown sample. Learning Objectives � Development of the laboratory skills related to weighing samples, making solutions and measuring freezing point depression values � To increase the understanding of the colligative properties of electrolytes and non-electrolytes � Development of computer skills in excel. Introduction Depression of freezing point is one of the colligative properties easiest to measure (others are vapour pressure, boiling point elevation, osmotic pressure). Colligative properties depend on the number of particles present in a solution. The equation for depression of freezing point ...
http://www.markedbyteachers.com/gcse/science/freezing-point-depression.html
Genetic Analysis of nif Regulatory Genes by Utilizing the Yeast Two-Hybrid System Detected Formation of a NifL-NifA Complex...Genetic Analysis of nif Regulatory Genes by Utilizing the Yeast Two-Hybrid System Detected Formation of a NifL-NifA Complex...
a The β-galactosidase activity assay was performed as described in the MATCHMAKER two-hybrid system (20). Briefly, cells from 1.5-ml samples of exponential culture were collected by centrifugation and resuspended in 300 μl of Z-buffer (20). A 100-μl aliquot of the resuspended cells was lysed by quick freeze-thaw (treatment with liquid nitrogen followed by thawing at 37°C). To measure the β-galactosidase activity in the cell lysate, a 0.7-ml sample of the Z-buffer-β-mercaptoethanol solution was added to each tube followed by 0.16 ml of Z-buffer-ONPG (4 mg of ONPG per 1 ml of Z-buffer). The time of ONPG addition was recorded, and the tubes were incubated at 30°C with shaking. When yellow color was visible, 400 μl of 1 M NaCO3 was added to each tube to terminate the reaction, and the time was recorded. The tubes were then centrifuged for 10 min at 10,000 × g to remove cellular debris, and the optical density at 420 nm was recorded. β-Galactosidase units were defined as the amount of ...
https://jb.asm.org/content/181/20/6535/figures-only
Techinjector Liquid Nitrogen Injector - Technifab CryogenicsTechinjector Liquid Nitrogen Injector - Technifab Cryogenics
Liquid nitrogen expands 700 times in volume when changing from liquid to gas. By injecting a small amount of liquid nitrogen just before the container is sealed, the expanding nitrogen gas will displace the air in the container. This creates an oxygen free (inert) environment to extend product life and can give a slight pressurization to thin wall packages if needed to improve container durability.. A continuous stream liquid nitrogen injection can be wasteful at slower line speeds and will not detect missing containers or the complete lack of containers on the packaging line. Technifabs Techinjector, using a sensor on the packaging line, detects each container and precisely injects a measured dose of liquid nitrogen at the proper time. The Programmable Logic Controller (PLC) unit automatically adjusts for line speed changes and does not dose missing containers. The unit can also be set up to run in a continuous mode and then shut off when containers stop coming down the production line.. The ...
http://technifab.com/cryogenics/cryogenic-products/techinjector-liquid-nitrogen-injector/
Sub-Zero - BI-30U/S/PH-RH - 30 Classic Over-and-Under Refrigerator/Freezer -BI-30U/S/PH-RH | D&K Appliances Inc.Sub-Zero - BI-30U/S/PH-RH - 30 Classic Over-and-Under Refrigerator/Freezer -BI-30U/S/PH-RH | D&K Appliances Inc.
Shop Sub-Zero - BI-30U/S/PH-RH - 30 Classic Over-and-Under Refrigerator/Freezer -BI-30U/S/PH-RH by Sub-Zero at D&K Appliances Inc. Sub-Zero Appliances Visit our showroom or call (610) 356-2424
https://www.dkappliances.com/Sub-Zero-BI-30U-S-PH-RH-30-Classic-Over-and-Under-Refrigerator-Freezer?manufacturer_id=2_52&page=1&limit=15
Egg Freezing: Dr. Diana Chavkin Answers the Crucial QuestionsEgg Freezing: Dr. Diana Chavkin Answers the Crucial Questions
Egg freezing has become more accepted over the past few years as a method of fertility preservation for a number of reasons. Before 2013, anyone who froze their eggs had to do so under an experimental protocol. But after studying egg freezing in depth, the American Society of Reproductive Medicine issued a statement that there is enough scientific evidence showing that egg freezing works and that it should longer be considered experimental.. Two important factors influenced this position: First, new egg freezing technology was introduced called vitrification, a fast-freeze method that almost instantly transforms the eggs into a glass-like frozen state. Vitrification reduces the likelihood that the fluid in the egg will form ice crystals, which could damage it. Multiple studies of egg freezing done via vitrification demonstrated that it was much more successful than the older method called slow freeze.. Second, a number of studies were done using donor eggs where it was reliably determined that ...
http://www.havingbabies.com/blog-list/Egg-feezing-dr-diana-chavkin-answers-the-crucial-questions/?format=amp
Direct linkDirect link
The quality of clinical biobank samples is crucial to their value for life sciences research. A number of factors related to the collection and storage of samples may affect the biomolecular composition. We have studied the effect of long-time freezer storage, chronological age at sampling, season and month of the year and on the abundance levels of 108 proteins in 380 plasma samples collected from 106 Swedish women. Storage time affected 18 proteins and explained 4.8-34.9% of the observed variance. Chronological age at sample collection after adjustment for storage-time affected 70 proteins and explained 1.1-33.5% of the variance. Seasonal variation had an effect on 15 proteins and month (number of sun hours) affected 36 proteins and explained up to 4.5% of the variance after adjustment for storage-time and age. The results show that freezer storage time and collection date (month and season) exerted similar effect sizes as age on the protein abundance levels. This implies that information on ...
http://www.diva-portal.org/smash/record.jsf?pid=diva2%3A1060041
Freezer Meal Pro - Freezer Meal ProFreezer Meal Pro - Freezer Meal Pro
When I started blogging back in 2012, I noticed that the posts people loved the most were the ones about quick, healthy, freezer meals I made. I received dozens of comments asking if I had any other recipes I could share or where similar ones might be.. I realized that I, or rather The Family Freezer, was the best friend that so many of my online readers needed.. They needed someone who could create, plan, and organize quick, healthy freezer recipes so that they wouldnt have to spend time doing that themselves. We know that everyone has distinct skills and strengths, well THESE are my skills! I am great at creating simple, healthy recipes for the freezer, and I am also great at organizing shopping lists and meal plans. I am your recipe-creating, list-making best friend!. ...
https://members.newleafwellness.biz/product/freezer-meal-pro-2/
Isothermal LN2 Freezers | Mandel ScientificIsothermal LN2 Freezers | Mandel Scientific
Custom BioGenic Systems offers only the highest quality in dry storage Isothermal Liquid Nitrogen Freezers. These models offer a wide lid opening to allow access to all racks, whilst providing an extensive variety of inventory solutions. We offer multi-tired straw storage systems, split samples, chest racks and cryo bag storage options. The vapour circulation provides better visability in the storage area.. These dry storage units offer additional user safety by eliminating liquid nitrogen contact. CBS dry storage units also minimize sample cross-contamination by eliminating liquid nitrogen submersion.. Mandel offers a complete selection of freezer sizes from 30L to 140L liquid nitrogen capacity options.. ...
https://www.mandel.ca/catalog/lab/labcryogenic/labcryogenicisothermal
Stainless Steel and Aluminum 2 and 3 Freezer Storage Racks - Western BlotStainless Steel and Aluminum 2 and 3 Freezer Storage Racks - Western Blot
Description: Standard 3 \ Stainless Steel Box w/Drain Holes Packaging: Bagged Size: 1g Qty: Dispenser with Slatwell Bracket 3 Unit: Pckg. of 12 Product: X-Gluc Quantity: 1 Tubes: 1.5/2.0ml L x W: 140 x 7mm Quantity/Unit: 10 Cell Size (mm/in
https://labscientific.com/western-blot/stainless-steel-and-aluminum-2-and-3-freezer-storage-racks
On the mechanism of freeze-induced crosslinking of aerogels made from periodate-oxidised cellulose nanofibrilsOn the mechanism of freeze-induced crosslinking of aerogels made from periodate-oxidised cellulose nanofibrils
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Fiber- och polymerteknologi. KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Centra, Wallenberg Wood Science Center.ORCID-id: 0000-0002-5444-7276 ...
http://kth.diva-portal.org/smash/record.jsf?pid=diva2%3A1277897&c=320&searchType=SIMPLE&language=sv&query=&af=%5B%5D&aq=%5B%5B%7B%22organisationId%22%3A%22879315%22%7D%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
Neuromics: 2013Neuromics: 2013
We are pleased to introduce a new application for one of our neural progenitor markers. In this study our Chicken Nestin Antibody is used to stain these progenitors in dissected mouse brains: Gleave JA, Lerch JP, Henkelman RM, Nieman BJ (2013) A Method for 3D Immunostaining and Optical Imaging of the Mouse Brain Demonstrated in Neural Progenitor Cells. PLoS ONE 8(8): e72039. doi:10.1371/journal.pone.0072039. 3D antibody staining of adult mouse brains: 1% PFA perfused adult mouse brains were removed from the skull and then divided by removing the cerebellum and separating the hemispheres if desired. The samples were immediately dehydrated in a gradient of methanol solutions to 100% methanol over the course of one day. Immediate dehydration is important to preserve the cellular morphology of the lightly-fixed brain. The samples then underwent freeze/thaw (one hour at -80°C/one hour at room temperature) four times. The samples were rehydrated in a gradient of methanol solutions to PBS over the ...
http://neuromics.blogspot.com/2013/
Liquid nitrogen - WikipediaLiquid nitrogen - Wikipedia
Because the liquid-to-gas expansion ratio of nitrogen is 1:694 at 20 °C (68 °F), a tremendous amount of force can be generated if liquid nitrogen is rapidly vaporized in an enclosed space. In an incident on January 12, 2006 at Texas A&M University, the pressure-relief devices of a tank of liquid nitrogen were malfunctioning and later sealed. As a result of the subsequent pressure buildup, the tank failed catastrophically. The force of the explosion was sufficient to propel the tank through the ceiling immediately above it, shatter a reinforced concrete beam immediately below it, and blow the walls of the laboratory 0.1-0.2 m off their foundations.[17]. Because of its extremely low temperature, careless handling of liquid nitrogen and any objects cooled by it may result in cold burns. In that case, special gloves should be used while handling. However, a small splash or even pouring down skin will not burn immediately because of the Leidenfrost effect, the evaporating gas thermally insulates to ...
https://en.m.wikipedia.org/wiki/Liquid_nitrogen
FreezingFreezing
Degree of frost Directional freezing Flash freezing Fractional freezing Freezer Freezing air temperature Frost Micro-pulling- ... Freezing is a phase transition where a liquid turns into a solid when its temperature is lowered below its freezing point. In ... freezing is one of the oldest and most widely used method of food preservation as far back as 1842, freezing has been immensely ... Experimental attempts to freeze human beings for later revival are known as cryonics. Freezing is a common method of food ...
https://en.wikipedia.org/wiki/Freezing
Freezing behaviorFreezing behavior
... or the freeze response or to be petrified is a reaction to specific stimuli, most commonly observed in prey ... Not only did these investigations show that the hippocampus is involved with freezing behavior, but avoidance and freezing ... That suggests that neurotransmitters, in general, play a role in freezing behavior. Several investigations show that freezing ... or freeze". In addition, freezing is observed to occur before or after a fight or flight response. Studies suggest that ...
https://en.wikipedia.org/wiki/Freezing_behavior
Asset freezingAsset freezing
... assets owned beneficially by someone else will not be subject to the freezing order. A freezing order is a precautionary ... A freezing order will usually only be made where the claimant can show that there was at least a good arguable case that they ... The freeze may be effected by issuing a letter to the asset holder or guardian in question, informing them of the true origin ... Asset freezing is a form of interim or interlocutory injunction which prevents a defendant to an action from dealing with or ...
https://en.wikipedia.org/wiki/Asset_freezing
Freezing PointFreezing Point
... may refer to: Freezing Point (novel), a 1965 Japanese novel by Ayako Miura Freezing Point, a 1967 South Korean ... the temperature of solid-liquid state change This disambiguation page lists articles associated with the title Freezing Point. ... film directed by Kim Soo-yong, based on the novel Freezing Point (magazine), a Chinese magazine Melting point, ...
https://en.wikipedia.org/wiki/Freezing_Point
Freezing toiletFreezing toilet
... is a kind of dry toilet powered by electricity which freezes the content in a short time. Freezing toilets work ... The function of freezing toilets is based on freezing. The contents freeze in a short time stopping bacteria from growing and ... Freezing toilets can be moved, as they only need a plug to work. The machine consumes about 40 watts of electricity.[citation ... "Toilet Freezes Your Poop To Keep It From Stinking". Gizmodo. Gawker Media. Retrieved 16 May 2015. "Literature - Käymäläseura ...
https://en.wikipedia.org/wiki/Freezing_toilet
Freezing (manga)Freezing (manga)
Freezing at Comic Valkyrie (in Japanese) Freezing: First Chronicle at Comic Valkyrie (in Japanese) Freezing: Zero at Comic ... Freezing Archived 2018-12-04 at the Wayback Machine at FUNimation Freezing at Seven Seas Entertainment Freezing (manga) at ... フリージングZERO 1 [Freezing: Zero] (in Japanese). ASIN 4799203045. "最新情報｜TVアニメ『フリージング ヴァイブレーション』公式サイト". freezing.tv. Archived from ... フリージング 1 [Freezing 1] (in Japanese). ASIN 4860324749. フリージング 30 [Freezing 30] (in Japanese). ASIN 4799209469. 프리징 1권
https://en.wikipedia.org/wiki/Freezing_(manga)
Freezing drizzleFreezing drizzle
Where freezing rain forms when frozen precipitation falls through a melting layer and turns liquid, freezing drizzle forms via ... Freezing drizzle is drizzle that freezes on contact with the ground or an object at or near the surface. Its METAR code is FZDZ ... When freezing drizzle accumulates on land, it creates an icy layer of glaze. Freezing drizzle alone does not generally result ... Although freezing drizzle and freezing rain are similar in that they both involve liquid precipitation above the surface in ...
https://en.wikipedia.org/wiki/Freezing_drizzle
Ground freezingGround freezing
Pipes are run through the soil to be frozen, and then refrigerants are run through the pipes, freezing the soil. Frozen soil ... "Ground Freezing: Freezing soil with liquid nitrogen". The Linde Group. 2014. Archived from the original on 2014-02-26. ... Ground freezing is a construction technique used in circumstances where soil needs to be stabilized so it will not collapse ... A project in Boston known as the Big Dig used ground freezing during some of its tunneling, to allow its wide tunnels to be ...
https://en.wikipedia.org/wiki/Ground_freezing
Freezing (disambiguation)Freezing (disambiguation)
Freezing air temperature Freezing of assets Freezing behavior Freeze (disambiguation) Frozen (disambiguation) Hang (computing) ... Freezing is the process in which a liquid turns into a solid when cold enough. Freezing may also refer to: Freezing (film), a ... a BBC comedy series Freezing, using local anesthetics in dentistry Frostbite, freezing of tissues Gait freezing, a common motor ... a biological condition in response to colder temperatures Directional freezing Freezing, the melting point of water, 0 °C (32 ° ...
https://en.wikipedia.org/wiki/Freezing_(disambiguation)
Surface freezingSurface freezing
... is the appearance of long-range crystalline order in a near-surface layer of a liquid. The surface freezing ... Surface freezing has since been found in a wide range of chain molecules and at various interfaces: liquid-air, liquid-solid ... Surface Freezing was experimentally discovered in melts of alkanes and related chain molecules in the early 1990s independently ... which did not allow a determination of the frozen layer's thickness, and whether or not it is laterally ordered. A group led by ...
https://en.wikipedia.org/wiki/Surface_freezing
Snap freezingSnap freezing
... (or cook-chill or blast freezing) is the process of rapid cooling of a substance for the purpose of preservation ... Snap frozen foods need to be packed in shallow trays to make the process more efficient. The food is cooled to a temperature ... Snap-freeze is a term often used in scientific papers to describe a process by which a sample is very quickly lowered to ... Cooked meals can be preserved by rapid freezing after cooking is complete. The main target group for these products are those ...
https://en.wikipedia.org/wiki/Snap_freezing
Directional freezingDirectional freezing
... freezes from only one direction. Directional freezing can freeze water, from only one direction or side of ... Directional freezing in a domestic freezer can be done by putting water in a insulated container so that the water freezes from ... Aquamelt Beluga whale Clear ice Hydrogel Freeze-casting§Static vs. dynamic freezing profiles Molecular self-assembly Zhang, ... "What is Directional Freezing?". Alcademics. Retrieved 12 October 2022. "Making clear ice at home with tap water and directional ...
https://en.wikipedia.org/wiki/Directional_freezing
Flash freezingFlash freezing
... is used in the food industry to quickly freeze perishable food items (see frozen food). In this case, food items ... Flash freezing techniques are used to freeze biological samples quickly so that large ice crystals cannot form and damage the ... "Freezing of fruits and vegetables". www.fao.org. Retrieved 2020-04-06. "Freezing water droplets form sharp ice peaks". ... In physics and chemistry, flash freezing is the process whereby objects are frozen in just a few hours by subjecting them to ...
https://en.wikipedia.org/wiki/Flash_freezing
Freezing toleranceFreezing tolerance
The ability to control intercellular ice formation during freezing is critical to the survival of freeze-tolerant plants. If ... The process of freezing tolerance through cold acclimation is a two-stage mechanism: The first stage occurs at relatively high ... Freezing tolerance can be assessed by performing a simple plant survival assay or with the more time consuming but quantitative ... Freezing tolerance is enhanced as a gradual adaptation to low temperature through a process known as cold acclimation, which ...
https://en.wikipedia.org/wiki/Freezing_tolerance
Fractional freezingFractional freezing
... as in freeze distillation, also called normal freezing or progressive freezing. The initial sample is thus fractionated ( ... Fractional freezing is generally used to produce ultra-pure solids, or to concentrate heat-sensitive liquids. Freeze ... Fractional freezing can be used to desalinate sea water. In a process that naturally occurs with sea ice, frozen salt water, ... fractional freezing can be useful due to its simplicity. Fractional freezing is also used in the production of fruit juice ...
https://en.wikipedia.org/wiki/Fractional_freezing
Freezing levelFreezing level
Above the freezing altitude, the temperature of the air is below freezing. Below it, the temperature is above freezing. The ... The freezing level, or 0 °C (zero-degree) isotherm, represents the altitude in which the temperature is at 0 °C (the freezing ... There are several different methods to examine the structure of the temperature of the atmosphere including its freezing level ...
https://en.wikipedia.org/wiki/Freezing_level
Freezing rainFreezing rain
... is rain maintained at temperatures below freezing by the ambient air mass that causes freezing on contact with ... Freezing rain often causes major power outages by forming glaze ice. When the freezing rain or drizzle is light and not ... Black ice Freezing drizzle Hail Ice pellets Ice storm Icing conditions List of ice storms "Freezing rain". Glossary of ... The METAR code for freezing rain is FZRA. A storm that produces a significant thickness of glaze ice from freezing rain is ...
https://en.wikipedia.org/wiki/Freezing_rain
Freezing Point (novel)Freezing Point (novel)
Freezing Point, a 1966 Japanese film directed by Satsuo Yamamoto Freezing Point (冰點), a 1966 Taiwanese film directed by Hsin ... a 1989 Japanese TV series on TV Asahi Freezing Point, a 2001 Japanese TV series on TV Asahi Freezing Point, a 2006 Japanese TV ... Freezing Point (氷点; Hyōten) is the debut novel of Japanese novelist Ayako Miura, first serialized on Asahi Shimbun between 1964 ... Miura, Ayako (1986). Freezing Point. Translated by Hiromu Shimizu; John Terry. Dawn Press. ISBN 0933704291. ...
https://en.wikipedia.org/wiki/Freezing_Point_(novel)
Freezing Point (magazine)Freezing Point (magazine)
Freezing Point was started in 1995 as a one-page publication and was expanded into a weekly magazine in 2004. A weekly ... Freezing Point (Chinese: 冰点, Bīngdiǎn) was a news journal in the People's Republic of China which was the subject of ... Freezing Point on China Digital Times "Chinese Journal Closed by Censors Is to Reopen", The New York Times, 16 February 2006. " ... The official reason for the January 2006 shutdown of Freezing Point was an article by history professor Yuan Weishi of Sun Yat- ...
https://en.wikipedia.org/wiki/Freezing_Point_(magazine)
Freezing spray advisoryFreezing spray advisory
"Freezing Spray Advisory". Glossary - National Weather Service. Retrieved 1 June 2011. National Weather Service. "Freezing Spray ... or else a heavy freezing spray watch, warning or a specific notice would be issued. The following is an example of a Freezing ... A Freezing Spray Advisory is issued by the National Weather Service of the United States to warn sea vessels and sometimes road ... THIS COMBINATION WILL POSE THE HAZARD OF FREEZING SPRAY OVER THE WATERS...MAINLY NEAR BELLINGHAM BAY...ROSARIO STRAIT...AND THE ...
https://en.wikipedia.org/wiki/Freezing_spray_advisory
Saskatoon freezing deathsSaskatoon freezing deaths
The Saskatoon freezing deaths were a series of suspicious deaths of Indigenous Canadians in and immediately outside of ... Razack, Sherene (January 2014). ""It Happened More Than Once": Freezing Deaths in Saskatchewan". Canadian Journal of Women and ... "New film renews community discussion about Aboriginal freezing deaths in Saskatoon". Dispatch. University of Regina. Archived ... The freezing deaths are discussed in podcasts such as Criminal and Commons. The Da Vinci's Inquest episode "The Sparkle Tour" ...
https://en.wikipedia.org/wiki/Saskatoon_freezing_deaths
Individual Quick FreezingIndividual Quick Freezing
... , usually abbreviated IQF, is a descriptive term for freezing methods used in the food processing ... One of the main advantages of this method of preparing frozen food is that the freezing process takes only a few minutes. The ... The food is in individual pieces, and is frozen quickly. Products commonly frozen with IQF technologies are typically smaller ... The short freezing prevents formation of large ice crystals in the product's cells, which destroys the membrane structures at ...
https://en.wikipedia.org/wiki/Individual_Quick_Freezing
Interim freezing orderInterim freezing order
An interim freezing order is a type of court order issued by a British court to freeze access to a person's property while they ... Unexplained wealth order Asset freezing Criminal Finances Act 2017 "Circular 003/2018: unexplained wealth orders". GOV.UK. ...
https://en.wikipedia.org/wiki/Interim_freezing_order
Freezing rain advisoryFreezing rain advisory
A FREEZING RAIN ADVISORY MEANS THAT PERIODS OF FREEZING RAIN OR FREEZING DRIZZLE WILL FORM A GLAZE ON ROADS RESULTING IN ... FREEZING DRIZZLE AND FREEZING FOG WILL DEVELOP THIS AFTERNOON AS WARM AIR RIDES UP OVER TOP OF COLD AIR TRAPPED AT THE SURFACE ... A freezing rain advisory was an advisory issued by the National Weather Service in the United States when freezing rain or ... AS WARM AIR ALOFT OVERRIDES COLD AIR AT THE SURFACE...FREEZING DRIZZLE AND FREEZING FOG WILL DEVELOP. WHILE WIDESPREAD ...
https://en.wikipedia.org/wiki/Freezing_rain_advisory
Westfield Freezing WorksWestfield Freezing Works
Anand Satyanand David Lange Phil Goff Westfield Freezing Works Westfield Freezing Works Westfield Freezing Works Filling and ... The Westfield Freezing Works was a large meat processing facility in the Auckland suburb of Westfield, New Zealand. The site ... During World War I a number of freezing works opened across the country to meet the demand for New Zealand produce. Prime ... "Westfield freezing works". teara.govt.nz. Retrieved 2019-11-18. "Otahuhu A&P Showgrounds - Discover - STQRY". discover.stqry. ...
https://en.wikipedia.org/wiki/Westfield_Freezing_Works
Freezing air temperatureFreezing air temperature
Freezing or frost occurs when the air temperature falls below the freezing point of water (0 °C, 32 °F, 273 K). This is usually ... Freezing (the physical aspect) Permafrost (permanently frozen ground) Frost and Rime (deposit of ice on cold surfaces) ... The primary symptom of frost weather is that water freezes. If the temperature is low for sufficiently long time, freezing will ... "freezing" for temperature of air below the freezing point of water "hoar frost" and "ground frost" for deposit of ice on cold ...
https://en.wikipedia.org/wiki/Freezing_air_temperature
Patea Freezing WorksPatea Freezing Works
The Patea Freezing Company came into being in 1910. In 1933, a new Patea Freezing Company was formed and at times provided work ... The Patea Freezing Works was a meat-processing plant promoted by The Patea A&P Association in the town of Patea. The West Coast ... At the time The Patea Freezing Works was owned by the British Vestey Group. Since the closure the site had fallen derelict. ... On Wednesday 6 February 2008 a large part of the Patea Freezing Works was destroyed by fire. During August 2009 Nikau ...
https://en.wikipedia.org/wiki/Patea_Freezing_Works
The Freezing AtlanticThe Freezing Atlantic
... is Aberdeen City's debut album. Its first single is "God Is Going to Get Sick of Me." It was produced by ...
https://en.wikipedia.org/wiki/The_Freezing_Atlantic
Freezing-point depressionFreezing-point depression
... is a drop in the minimum temperature at which a substance freezes, caused when a smaller amount of ... The freezing point depression thus is called a colligative property. The explanation for the freezing point depression is then ... The freezing-point depression prevents radiators from freezing in winter. Road salting takes advantage of this effect to lower ... defined as the freezing point T f 0 {\displaystyle T_{f}^{0}} of the pure solvent minus the freezing point T f {\displaystyle T ...
https://en.wikipedia.org/wiki/Freezing-point_depression
Freezing (TV series)Freezing (TV series)
Freezing at IMDb Freezing at BBC Online British Comedy Guide Freezing reviews from Tom Hollander Fan Site v t e (Articles with ... Freezing was originally a one-off comedy as part of BBC Four's Tight Spot season in February 2007, which then became the first ... Freezing is written by James Wood and directed by Simon Curtis. Hugh Bonneville as Matt Elizabeth McGovern as Elizabeth Tom ... Freezing is a BBC comedy series starring Hugh Bonneville and Elizabeth McGovern about an otherwise successful couple in their ...
https://en.wikipedia.org/wiki/Freezing_(TV_series)
Passengers Push Plane In Below Freezing Weather : NPRPassengers Push Plane In Below Freezing Weather : NPR
Passengers Push Plane In Below Freezing Weather Amateur video captured the passengers giving it their all in arctic Russia this ... Passengers Push Plane In Below Freezing Weather. Listen · 0:29 0:29 ...
https://www.npr.org/transcripts/366961292
Max Dürstelers The Freezing Rotation Illusion. - NeatoramaMax Dürsteler's The Freezing Rotation Illusion. - Neatorama
Main Blog , Max Dürstelers The Freezing Rotation Illusion. Max Dürstelers The Freezing Rotation Illusion. ...
https://www.neatorama.com/2006/05/26/max-drstelers-the-freezing-rotation-illusion/
frozen pops - Fred Meyerfrozen pops - Fred Meyer
Find undefined at a store near you. Order undefined online for pickup or delivery. Find ingredients, recipes, coupons and more.
https://www.fredmeyer.com/q/frozen+pops
Auburn officially hires Hugh Freeze as head coachAuburn officially hires Hugh Freeze as head coach
Freeze won 39 games at Ole Miss. The Rebels were 5-7 in Freezes final season with the school. Ole Miss won seven games in his ... Hugh Freezes SEC return is here.. Auburn announced Monday that it has hired Freeze, the ex-Ole Miss coach who most recently ... Freeze resigned from Ole Miss ahead of the 2017 season after it was revealed he made calls to escorts from his Ole Miss phone. ... Freeze ultimately compiled a 39-25 record over five seasons at Ole Miss and the teams recruiting ticked up during his time ...
https://sports.yahoo.com/auburn-officially-hires-hugh-freeze-as-head-coach-232736140.html
Kashi GO Expands In Frozen Aisle With New Line Of Protein WafflesKashi GO Expands In Frozen Aisle With New Line Of Protein Waffles
Frozen Cookie Cakes : ice cream cakeFrozen Cookie Cakes : ice cream cake
To make this delicious frozen cake, begin by baking two circular sheets of brownies from a box. Then layer the first brownie ...
https://www.trendhunter.com/trends/ice-cream-cake
Frozen Sun company - Indie DBFrozen Sun company - Indie DB
Link to Frozen Sun by selecting a button and using the embed code provided more.... ...
https://www.indiedb.com/company/frozen-sun
If your iPod wont turn on or seems frozen - Apple Support (GU)If your iPod won't turn on or seems frozen - Apple Support (GU)
If your iPod wont turn on or seems frozen. Use this article for help with your iPod classic, iPod nano, and iPod shuffle. ...
https://support.apple.com/en-gu/HT204688
Mens Star Wars Han Solo Carbonite Frozen Poster Graphic TeeMen's Star Wars Han Solo Carbonite Frozen Poster Graphic Tee
Your Star Wars fan will love showing off his fandom with this Mens Star Wars Han Solo Carbonite Frozen Poster Graphic Tee. ... Your Star Wars fan will love showing off his fandom with this Mens Star Wars Han Solo Carbonite Frozen Poster Graphic Tee. ... Your Star Wars fan will love showing off his fandom with this Mens Star Wars Han Solo Carbonite Frozen Poster Graphic Tee. ... Your Star Wars fan will love showing off his fandom with this Mens Star Wars Han Solo Carbonite Frozen Poster Graphic Tee. ...
https://www.kohls.com/product/prd-3949429/target=
Ikeas Best Packaged And Frozen Foods - Todays ParentIkea's Best Packaged And Frozen Foods - Today's Parent
The Frozen Waffles. Hey Stranger Things, theres a new waffle in town. If youve had aspirations of making your own waffles, ... These rösti (similar to latkes or hash browns) come in pancake-size portions and are baked (or fried) from frozen. Hot tip: a ... The market sells prepared foods you can take home, everything from drinks to preserves, candy and frozen dishes, all based on ... Here are the best packaged and frozen food items at Ikea Canada. ...
https://www.todaysparent.com/family/ikea-canada-best-food-market-packaged-frozen/
Re: Code freeze break request for gnome-shellRe: Code freeze break request for gnome-shell
MedlinePlus: Frozen Strawberry YogurtMedlinePlus: Frozen Strawberry Yogurt
This refreshing frozen strawberry yogurt is quick and easy to make. Its a creamy, sweet treat that tastes just like a ... Serve immediately or freeze in an air-tight container or frozen treat molds for up to 1 month. Frozen leftovers will be very ... Combine frozen strawberries, yogurt and sugar in a blender or food processor. Blend until creamy, about 2 to 4 minutes. ... Frozen berries are a good choice when strawberries are not in season. Look for those with no sugar added. ...
https://medlineplus.gov/recipes/frozen-strawberry-yogurt/
Photos: Snow, sleet, freezing rain, and plain rain on tap for SundayPhotos: Snow, sleet, freezing rain, and plain rain on tap for Sunday
... ... Photos: Snow, sleet, freezing rain, and plain rain on tap for Sunday. ...
https://www.wtvr.com/news/local-news/photos-snow-sleet-freezing-rain-and-plain-rain-on-tap-for-sunday
Glitter Frozen 2 Cupcake Kit for 24 | Party CityGlitter Frozen 2 Cupcake Kit for 24 | Party City
The Glitter Frozen 2 Cupcake Kit for 24 includes blue glittery cupcake wrappers, matching two-inch baking cups, and Anna and ... The Glitter Frozen 2 Cupcake Kit for 24 includes blue glittery cupcake wrappers, matching two-inch baking cups, and Anna and ... Give a magical touch to your treats with a Glitter Frozen 2 Cupcake Kit for 24! Blue glittery cupcake wrappers, matching baking ... Glitter Frozen 2 Cupcake Kit for 24. Paper Cupcake Wrappers, Baking Cups & Wood Picks ...
https://www.partycity.com/glitter-frozen-2-cupcake-kit-for-24-863779.html?cgid=frozen-party-supplies&fromplp=true
Frozen Foot Five Mile - Road Runners Club of AmericaFrozen Foot Five Mile - Road Runners Club of America
1501 Langston Blvd, Ste 140 Arlington, VA 22209 ...
https://www.rrca.org/event/frozen-foot-five-mile-2/
Banquet Popcorn Chicken | 24 Oz. | Frozen Chicken & Turkey | HannafordBanquet Popcorn Chicken | 24 Oz. | Frozen Chicken & Turkey | Hannaford
Banquet Popcorn Chicken found at Hannaford Supermarket. Add to online shopping list or grocery cart for Hannaford To Go.
https://www.hannaford.com/product/banquet-popcorn-chicken/1010839
frozen food Archives - NaturalCures.comfrozen food Archives - NaturalCures.com
... frozen food blog offers natural treatments for over 250 diseases and ailments. Try them out and see if they work for you. ... Frozen Food NaturalCures Aug 5, 2019. 0 Description: Frozen food entrees and meals, seafood and meats, meat-free foods, gourmet ... What to look for: Certified organic foods that are preferably flash frozen… ... frozen soups, juices, fruits and vegetables, baby food, desserts and breads. ...
https://naturalcures.com/tag/frozen-food/
Multimodal Balance Training Improves Freezing of Gait in PDMultimodal Balance Training Improves Freezing of Gait in PD
Multimodal balance training with rhythmic cues improved balance and freezing of gait with persistent benefit in Parkinsons ... Freezing and Balance Freezing and loss of balance can both limit mobility and may lead to injury. Nonpharmacologic ... NICE, France - Both Parkinsons patients who have freezing of gait, known as "freezers," and those who do not, "non-freezers," ... Cite this: Multimodal Balance Training Improves Freezing of Gait in PD - Medscape - Sep 25, 2019. ...
http://www.medscape.com/viewarticle/918979
Hepatitis A infections linked to frozen strawberries | CDCHepatitis A infections linked to frozen strawberries | CDC
... contamination was found in multiple samples of ICAPP frozen strawberries, which were then recalled. This investigation is over. ... Frozen Strawberry Recall. *On October 30, 2016, ICAPP announced that it had notified its distributors that frozen strawberries ... If the facility has received any of the recalled frozen strawberries but has not served them to anyone, any remaining product ... If the facility has served any of the recalled frozen strawberries in the last 2 weeks since the notification , contact their ...
https://www.cdc.gov/hepatitis/outbreaks/2016/hav-strawberries.htm
Freezing Cabbage | HGTVFreezing Cabbage | HGTV
Learn how to freeze this nutrient-rich vegetable from the experts at HGTV Gardens. ... How to Freeze Vegetables, Fruit and More 22 Photos Learn how to freeze vegetables, fruit and even eggs and milk, and see how ... Can You Freeze Celery? Home-grown celery has a bold flavor thats tough to beat. Preserve that tangy flavor by freezing celery- ... How to Freeze Broccoli Freeze your surplus of broccoli now and enjoy the health benefits of this vegetable for months to come. ...
https://www.hgtv.com/outdoors/gardens/garden-to-table/freezing-cabbage
Sustainable reuse project continues after EPA freeze - The OracleSustainable reuse project continues after EPA freeze - The Oracle
Complications occurred with administrative transition when the Donald Trump Administration issued a freeze on all grants ... though the temporary freeze did not cause any delays and the project is still scheduled to begin in March. ... regional officials at state and local agencies received no prior warning or follow-up notice from the EPA about the freeze, ...
https://www.usforacle.com/2017/02/05/sustainable-reuse-project-continues-after-epa-freeze/
Below-freezing temperatures expected in parts of LA County, chance of rain on the wayBelow-freezing temperatures expected in parts of LA County, chance of rain on the way
Below-freezing temperatures expected in parts… Share this:. *Click to share on Facebook (Opens in new window) ... Below-freezing temperatures expected in parts of LA County, chance of rain on the way ...
https://www.presstelegram.com/2022/11/29/below-freezing-temperatures-expected-in-parts-of-la-county-chance-of-rain-on-the-way/
Canning/Freezing - Home CookingCanning/Freezing - Home Cooking
One of the best ways to savor the seasons is to can or freeze! This not only saves us money when produce is off-season, but we ... How to Freeze Green Beans Freezing is the easiest way to preserve fresh summer green beans to enjoy all year long. For the ... Vegetable Freezing Chart Most fresh vegetables must be "blanched" before they are frozen. Blanching is a two-step process... ... One of the best ways to savor the seasons is to can or freeze! This not only saves us money when produce is off-season, but we ...
https://www.bellaonline.com/subject.asp?cat=456
Egg Freezing Archives - LifeSiteEgg Freezing Archives - LifeSite
Will egg freezing liberate women? Egg freezing companies are hosting egg freezing cocktail parties. Seriously. ... Articles tagged Egg Freezing. Jennifer Anistons failed bid to have children exposes the lie that well always be young Have a ... Facebook, Apple pay for female employees to freeze eggs: what does this say to women? My jaw hit the ground when I read the ...
https://www.lifesitenews.com/tags/tag/egg-freezing/
Frozen | EW.comFrozen | EW.com
First sneak peek of new Frozen short Frozen Fever arrives Article // February 02, 2015 ... How one Frozen animator is keeping the Disney magic alive from quarantine With his Olaf short series, Hyrum Osmond shows how ... Frozen Fever to make its Disney Channel debut in time for the holidays Article // November 04, 2015 ... Frozen reunion! Kristen Bell, Idina Menzel, Josh Gad get together for charity Article // March 14, 2016 ...
https://ew.com/creative-work/frozen/
Spiced Pear Jam Frozen Yogurt - McEvoy RanchSpiced Pear Jam Frozen Yogurt - McEvoy Ranch
The spiced pear flavor really comes through and this yummy frozen yogurt made an outstanding sandwich with ginger snap ... Freeze according to the manufacturers instructions for your ice-cream maker. When the yogurt is sufficiently frozen, transfer ... Go ahead - make yourself and your kids happy! Jammy Frozen Yogurt Makes 5 cups frozen yogurt Ingredients 3 cups unsweetened ... The spiced pear flavor really comes through and this yummy frozen yogurt made an outstanding "sandwich" with ginger snap ...
https://www.mcevoyranch.com/blogs/recipes-and-more/spiced-pear-jam-frozen-yogurt
Boiron Lychee Frozen Puree 1k - Surfas OnlineBoiron Lychee Frozen Puree 1k - Surfas Online
One of our favorites for a Lychee martini! Sweet and refreshing Lychee fruit puree by Boiron of France is made from meticulously selected lychees made with a pasteurizing process of rapid heating and cooling that preserves the fresh taste and color. Defrost the puree in the refrigerator for 24 to 48 hours before using,
https://www.surfasonline.com/products/frzn-puree-boiron-lychee-1k
Frozen Produce Nutrition Versus Fresh Produce | PreventionFrozen Produce Nutrition Versus Fresh Produce | Prevention
... weve pitted frozen produce against fresh-and were shocked by what the numbers revealed. Check out our latest Health Food Face- ...
https://www.prevention.com/food-nutrition/healthy-eating/a20466243/frozen-produce-nutrition-versus-fresh-produce/
CalorieKing - Low Fat Recipes and Low Carb Recipes - Frozen Yogurt Apricot FloatCalorieKing - Low Fat Recipes and Low Carb Recipes - Frozen Yogurt Apricot Float
Frozen Yogurt Apricot Float - Low carb and Low fat recipes, Full of flavor and healthier than the traditional ice cream float, ... If melba frozen yogurt isnt available, or for variety, use peach or orange frozen low-fat yogurt or sorbet. If desired, you ... Frozen Yogurt Apricot Float. Full of flavor and healthier than the traditional ice cream float, this Recipe Makeover is mmm... ... By replacing the regular soft drink with the natural sweetness of fruit nectar, and the full-fat ice cream with low-fat frozen ...
https://www.calorieking.com/recipes/Recipe-Makeovers/Healthy-Recipe-Makeovers/Frozen-Yogurt-Apricot-Float_Y2lkPTM5JnNpZD03MSZyaWQ9ODk5.html
Tracing and freezing cryptocurrency in Hong Kong and Singapore | Perspectives | Reed Smith LLPTracing and freezing cryptocurrency in Hong Kong and Singapore | Perspectives | Reed Smith LLP
Freezing injunction: This is a freezing order (also known as a Mareva injunction) which may be granted in Hong Kong and ... Home Perspectives Tracing and freezing cryptocurrency in Hong Kong and Singapore With the rise of cryptocurrency, fraud ... including a freezing injunction to freeze the account held at the cryptocurrency exchange. ... Proprietary declarations and vesting orders: Once monies are frozen in place, the court can vest monies in, or declare monies ...
https://www.reedsmith.com/en/perspectives/2021/08/tracing-and-freezing-cryptocurrency-in-hong-kong-and-singapore
SearchVegetables20172016Green BeansStrawberriesSleetEggsBlanchYogurtMeltingEmbryo transferPreserveCrystalsDeep freezeTemperaturesAdhesive capsulitisFreshThawAirtightFoodTimeLikely source of this outbreakIngredientsFreezer bagsLeftoversRecipesFreshnessFlavorPortionsApproachesTendSpotsDouble freezeAdvancesGroceryBowlFertilityDisney FrozenOlafVictoryMeatsCabbageParchmentBlenderDegrees
Search2
  • After a thoughtful, thorough, and well-vetted search, we ended where we started, with Hugh Freeze,' Auburn athletic director John Cohen said in a statement. (yahoo.com)
  • Search for recipes online, and try them out on your family before planning to freeze a large batch. (hgtv.com)
Vegetables9
  • clickable map to find pick your own farms and orchards for fruit, vegetables, pumpkins and canning & freezing instructions! (pickyourown.org)
  • Learn how to freeze vegetables, fruit and even eggs and milk, and see how easy it is to extend the life of perishable foods. (hgtv.com)
  • Learn methods for freezing vegetables so you can have fresh taste long after the growing season ends. (hgtv.com)
  • Most fresh vegetables must be "blanched" before they are frozen. (bellaonline.com)
  • Research shows that the nutrient profiles of frozen fruits and vegetables are nearly equivalent to those of fresh produce. (nrdc.org)
  • One exception is fresh fruits and vegetables-those usually require a quick blanch or purée beforehand, and you should not freeze them if they have been left out of the refrigerator for more than six hours. (nrdc.org)
  • Frozen peas are one of the most popular frozen vegetables, and have been since Clarence Birdseye discovered in the 1920s that peas quickly blanched before being frozen result in a vividly green pea. (wired.com)
  • He experimented with freezing vegetables and caribou meat, simply as a matter of ensuring he had enough to eat. (wired.com)
  • Similarly, compared to refined grains with trans fats, refined grains without trans fat significantly increased snack price, as did fruit and canned or frozen vegetables. (cdc.gov)
20171
  • Freeze resigned from Ole Miss ahead of the 2017 season after it was revealed he made calls to escorts from his Ole Miss phone. (yahoo.com)
20163
  • On August 8, 2016, Tropical Smoothie Café reported that they removed the Egyptian frozen strawberries from their restaurants in Maryland, North Carolina, Virginia, and West Virginia and switched to another supplier out of an abundance of caution. (cdc.gov)
  • On October 30, 2016, the International Company for Agricultural Production & Processing (ICAPP) recalled all of its frozen strawberries that were imported into the U.S. since January 1, 2016. (cdc.gov)
  • On October 30, 2016, ICAPP announced that it had notified its distributors that frozen strawberries were being recalled due to a potential contamination with hepatitis A. (cdc.gov)
Green Beans9
  • Freezing is the easiest way to preserve fresh summer green beans to enjoy all year long. (bellaonline.com)
  • It was just plain easier for Mom to drop a block of frozen Birds Eye French-cut green beans out of the cardboard box and into a pot of boiling water. (wired.com)
  • Times have changed dramatically: Mom wouldn't think of buying frozen green beans now-instead she buys fresh haricots verts, the slender and tender green bean, available year-round at her local Publix. (wired.com)
  • Freezing Squash and Green Beans? (thriftyfun.com)
  • I specifically want to know how to preserve fresh squash and green beans I do not like what freezing does to them they have no taste when cooked.If any one can help please answer. (thriftyfun.com)
  • If you choose to freeze green beans it's best if you blanch them first, then lay them out on a cookie sheet in the freezer till they are frozen and bag. (thriftyfun.com)
  • what if you just freeze fresh green beans without blanching first. (thriftyfun.com)
  • I do not freeze green beans. (thriftyfun.com)
  • I cook green beans, and cool completely, then freeze in zip lock bags. (thriftyfun.com)
Strawberries8
  • Combine frozen strawberries, yogurt and sugar in a blender or food processor. (medlineplus.gov)
  • Frozen berries are a good choice when strawberries are not in season. (medlineplus.gov)
  • Epidemiologic and traceback evidence indicate frozen strawberries imported from Egypt were the likely source of this outbreak. (cdc.gov)
  • FDA traceback information indicated that the frozen strawberries served in the Tropical Smoothie Café locations were from the International Company for Agricultural Production & Processing (ICAPP), imported from Egypt. (cdc.gov)
  • The FDA reports that hepatitis A virus contamination was found in multiple samples of ICAPP frozen strawberries. (cdc.gov)
  • Facilities and retailers should not sell or serve recalled frozen strawberries. (cdc.gov)
  • If the facility has received any of the recalled frozen strawberries but has not served them to anyone, any remaining product should be discarded and no further action is needed. (cdc.gov)
  • the origin of the epidemic was traced to contaminated frozen strawberries. (cdc.gov)
Sleet4
  • Are Sleet and Freezing Rain the Same Thing? (wral.com)
  • RALEIGH - Are the terms sleet and freezing rain interchangeable? (wral.com)
  • The raindrop falls into a layer of cold air and then re-freezes and turn into little ice pellets - what is called sleet. (wral.com)
  • Some Triangle residents tend to dismiss both sleet and freezing rain as non-events, believing if there is no snow, there is no danger - that is a mistake. (wral.com)
Eggs11
  • But there's an art in knowing what - and how - to freeze fruits, veggies and everything from milk to herbs to eggs. (hgtv.com)
  • Facebook, Apple pay for female employees to freeze eggs: what does this say to women? (lifesitenews.com)
  • For women at risk of losing their fertility through medical treatments or premature menopause, preserving some of their eggs ahead of time by freezing them can leave open the possibility of having children. (scientificamerican.com)
  • Eggs generally deteriorate during the freezing process because they are not sufficiently dehydrated. (scientificamerican.com)
  • Rafaella Fabbri of the University of Bologna and her colleagues found that increasing the amount of time the eggs were exposed to chemical protectants and boosting the amount of sugar in the freezing solution improved dehydration. (scientificamerican.com)
  • There have been huge advances in egg freezing, and now frozen eggs are almost as viable as fresh ones as long as the fertility specialist knows what he or she is doing. (cnn.com)
  • If you're not ready to have a baby in your 20s, or early- to mid-30s, harvesting and freezing your eggs is a great option for women who want to postpone motherhood. (cnn.com)
  • What if electively freezing one's eggs is not a means of empowerment but a surrender to corporate control? (newyorker.com)
  • While the successful freezing of human semen and embryos had been possible for decades, eggs proved much more fragile, prone to forming ice crystals that rendered them unviable. (newyorker.com)
  • By 2012, the evidence was in: scientists determined that, for young women, eggs that have been frozen using these methods are just as likely to result in pregnancy and the birth of a healthy child as ones freshly harvested in a cycle of in-vitro fertilization. (newyorker.com)
  • What if, rather than being a means of empowerment-whereby a young woman is no longer subject to anything so quaintly analog as the ticking of a biological clock-freezing one's eggs is understood as a surrender to the larger, more invisibly pervasive force of corporate control? (newyorker.com)
Blanch3
  • For the longest-lasting frozen cabbage, blanch wedges for 90 seconds. (hgtv.com)
  • I then blanch them, let them dry a little, freeze them on cookie sheets and package them in vaccum seal bags. (thriftyfun.com)
  • Almost every method of freezing summer squash says to blanch it. (thriftyfun.com)
Yogurt8
  • The spiced pear flavor really comes through and this yummy frozen yogurt made an outstanding "sandwich" with ginger snap cookies. (mcevoyranch.com)
  • Jammy Frozen Yogurt Makes 5 cups frozen yogurt Ingredients 3 cups unsweetened whole milk yogurt (not Greek style) 1 cup granulated sugar 1/2 cup jam (I used McEvoy Ranch Spiced Pear Jam ) 1/4 teaspoon fine sea salt Directions Pour the yogurt, sugar, jam, and salt into a blender and puree. (mcevoyranch.com)
  • When the yogurt is sufficiently frozen, transfer it to an airtight container, cover tightly, and freeze. (mcevoyranch.com)
  • By replacing the regular soft drink with the natural sweetness of fruit nectar, and the full-fat ice cream with low-fat frozen yogurt, this float is a refreshing treat for the whole family! (calorieking.com)
  • Top with frozen yogurt. (calorieking.com)
  • If melba frozen yogurt isn't available, or for variety, use peach or orange frozen low-fat yogurt or sorbet. (calorieking.com)
  • 5. Working quickly, use a small ice cream scoop to form small, bite-sized pieces of frozen yogurt and place on a parchment-lined baking sheet. (buzzfeed.com)
  • Dip the frozen yogurt balls in the melted chocolate and place back on a parchment-lined baking sheet. (buzzfeed.com)
Melting3
  • Since melting snow stays at freezing, the melting layer is often at a uniform temperature of 32°F. Eventually the snow melts completely and we reach the snow level, below which only rain is observed. (rei.com)
  • And then there is melting: When snow falls into air warmer than freezing, it melts. (rei.com)
  • Melting thus causes the freezing and snow levels to fall. (rei.com)
Embryo transfer2
  • ABSTRACT This study in Turkey evaluated the impact of age-based mandatory single-embryo transfer (SET) legislation with the subsequent increase in frozen-thawed embryo transfer (FT-ET) on pregnancy outcome of in vitro fertilization (IVF) patients. (who.int)
  • Frozen Embryo Transfer in IVF--As Good as Fresh? (medscape.com)
Preserve1
  • Preserve that tangy flavor by freezing celery-we'll explain how. (hgtv.com)
Crystals4
  • Freezing water forms a spiky kaleidoscope of colorful crystals. (exploratorium.edu)
  • A team led by Roger Gosden at McGill University in Montreal bathed eight complete rat ovaries in a solution containing fructose and dimethylsulphoxide - chemicals designed to prevent large, destructive ice crystals forming between cells during freezing. (newscientist.com)
  • And with freezing, he paid particular attention to the size of the ice crystals relative to the way the fish or meat was frozen. (wired.com)
  • He recognized that when the crystals were large, a result of slow freezing, they damaged the cell structure, the meat leaked juices, and its texture became mealy. (wired.com)
Deep freeze1
  • Learn how to prep onions for safe storage in the deep freeze. (hgtv.com)
Temperatures1
  • I am 10 years old and my granmother asked me what is the degree for freezing temperatures. (wral.com)
Adhesive capsulitis4
  • A frozen shoulder is the term that refers to a condition of the shoulder joint medically known as adhesive capsulitis . (medicinenet.com)
  • Frozen shoulder (adhesive capsulitis) develops as the result of scarring, thickening, and shrinkage of the joint capsule. (medicinenet.com)
  • A frozen shoulder (adhesive capsulitis) is when the shoulder joint experiences a significant loss in its range of motion due to. (medicinenet.com)
  • First of all it was a rotator cuff problem, it would get better, it would settle down, and then quite soon I must say, I was diagnosed with what was called a Frozen Shoulder, technical term, Adhesive Capsulitis. (abc.net.au)
Fresh11
  • Is Frozen As Healthy As Fresh? (prevention.com)
  • To find out, we've pitted frozen produce against fresh-and were shocked by what the numbers revealed. (prevention.com)
  • By freezing zucchini you'll have access to this fresh veggie year-round and won't have to worry about your bounty going to waste. (allrecipes.com)
  • But are frozen foods as good for you as fresh? (nrdc.org)
  • The technology of freezing fish has evolved to the point where it's comparable to, if not better than, fresh fish," explained chef Barton Seaver, who is also the director of the sustainable seafood and health initiative at Harvard's Center for Health and the Global Environment. (nrdc.org)
  • Freeze shelled almonds and walnuts to keep them fresh for up to two years. (ehow.com)
  • All nuts contain natural oils that become rancid over time when stored at room temperature, but freezing helps keep them fresh for longer. (ehow.com)
  • Fresh frozen plasma (FFP) is a blood component containing procoagulant factors, which is sometimes used in cardiovascular surgery with the aim of reducing the risk of bleeding. (nih.gov)
  • Treatment measures include fresh frozen plasma (FFP), prothrombin complex concentrates (PCCs), and vitamin K. Additionally, in patients with acquired factor II deficiency, the underlying cause should be found and treated. (medscape.com)
  • In this study, the steady-state flux and lag time of diethyl phthalate (DEP) were measured for fresh human skin and skin frozen at .85 degrees C for 1, 2, 3, 6, 9, 12, and 18 months with 10% glycerol as a cryoprotective agent. (cdc.gov)
  • No significant differences in steady-state flux were found between fresh and previously frozen samples (p = 0.6). (cdc.gov)
Thaw3
  • There's no need to thaw frozen zucchini when cooking. (allrecipes.com)
  • Abdelhadi explained that the cryoablation technique includes a triple-freeze protocol, opposed to double-freeze techniques: The tumor is frozen for 3 minutes, thawed for 6 minutes, frozen for 10 minutes, followed by a 13-minute thaw period, and finally frozen for 20 minutes. (medpagetoday.com)
  • This video explores what's happening to your food at a molecular level as it goes through a freeze-thaw cycle. (exploratorium.edu)
Airtight1
  • Some tips for becoming a successful food-freezing champion: Freeze in portions, use airtight containers to reduce freezer burn, and do a survey of what's in the freezer every once in a while, since quality does decline over time. (nrdc.org)
Food11
  • Here are the best packaged and frozen food items at Ikea Canada. (todaysparent.com)
  • During the Vietnam Conflict we started making freeze-dried food as Long Range Patrol rations for the military. (ofd.com)
  • In the 1960s, we started supplying freeze-dried food for simulated space missions and successfully sent our products into space on the Apollo missions, Skylab and joint U.S.-Russian space ventures. (ofd.com)
  • And despite its bad rap, frozen food-and freezing food -is one of the keys to cutting down on what you toss. (nrdc.org)
  • Less food goes to waste when it's shipped frozen, since it's not as perishable on the journey from the farm to your refrigerator. (nrdc.org)
  • For the most part, if frozen food has been thawed in the refrigerator, it's fine to refreeze it, though the quality might suffer a bit. (nrdc.org)
  • Birdseye was the father of frozen food, the man most responsible for what is now a $240 billion global industry. (wired.com)
  • As with so many of our food innovations, frozen food arose from the unpredictable mix of an eccentric, adventurous, inquisitive mind, a man's serendipitous move through the world (his interest in frozen food was rooted in fox farming in Labrador, in northeastern Canada, a place that gets mighty cold in the winter), and advances in technology and in packaging during the 1920s that allowed new ideas to find their physical realization. (wired.com)
  • Survival required him to learn all methods of preserving food-salting, drying, and freezing. (wired.com)
  • Freezing rain causes havoc with power lines, so it is best to be prepared with flashlights, food and logs for the fireplace. (wral.com)
  • CDC collaborated with public health and regulatory officials in several states and the U.S. Food and Drug Administration External (FDA) to investigate an outbreak of Salmonella Paratyphi B variant L(+) tartrate(+) and Salmonella Weltevreden infections linked to frozen raw tuna. (cdc.gov)
Time15
  • Freeze ultimately compiled a 39-25 record over five seasons at Ole Miss and the team's recruiting ticked up during his time with the team. (yahoo.com)
  • What results in a short amount of time is frozen hair. (kgab.com)
  • Good thing because you don't have time to waste at the end of a long, stressful, freezing day. (wearenotmartha.com)
  • Follow these four easy steps and you can freeze zucchini for a little taste of summer any time you want. (allrecipes.com)
  • Once your zucchini is cooled, it's time to move onto freezing. (allrecipes.com)
  • If you're in a rush, or really don't mind a little extra cooking time after freezing, you can simply tuck your blanched zucchini into zip-topped plastic bags and stick them in the freezer. (allrecipes.com)
  • The additional travel time allowed by freezing also means the fish can be shipped by land instead of air. (nrdc.org)
  • Entire frozen ovaries have been successfully thawed and transplanted for the first time. (newscientist.com)
  • My neighbor sliced her squash, dipped it in flour , layed it out in single pieces on a large tray , put in to the freezer until frozen then into ziplock bags for ever how many you wish to fry at a time. (thriftyfun.com)
  • Both the freezing level and snow level can change in time as precipitation falls, and the direction is usually down. (rei.com)
  • The National Weather Service forecasts often talk about freezing and snow levels and how they will change in time: information worth being aware of. (rei.com)
  • Haukaas conveys both a sense of fluid motion and frozen time in this image, a modern example of Native American ledger art (a genre of narrative drawing or painting on paper or cloth) that developed during the Indian Wars era and continued in the forced relocations of Plains tribes to government reservations from the 1860s through the 1920s. (cdc.gov)
  • As they reach puberty and encounter the incest-related threats it entails, some adolescents may be tempted to freeze the passage of time, in other words to stop temporal flow in an effort to take refuge from the drive's circuit. (bvsalud.org)
  • Thus, freezing time amounts to ensuring that nothing arises any longer, or indeed moves on. (bvsalud.org)
  • frozen time. (bvsalud.org)
Likely source of this outbreak1
  • Epidemiologic, laboratory, and traceback findings indicated that frozen raw tuna was the likely source of this outbreak. (cdc.gov)
Ingredients1
  • A clutch of top-notch bars and restaurants across the country are freezing rosé or blending it with ice, sweetening it up with ingredients like simple syrup and citrus, and making it into a refreshingly chilly drink. (robbreport.com)
Freezer bags2
  • After it's frozen, toss wedges into freezer bags in bulk. (hgtv.com)
  • Place in freezer bags and freeze. (thriftyfun.com)
Leftovers2
  • Frozen leftovers will be very firm. (medlineplus.gov)
  • Note that leftovers should be frozen within three to four days. (nrdc.org)
Recipes1
  • The market sells prepared foods you can take home, everything from drinks to preserves, candy and frozen dishes, all based on Swedish recipes and traditions. (todaysparent.com)
Freshness2
  • For the highest quality and nutrition, they should be frozen at the peak of freshness. (bellaonline.com)
  • Use bags or containers especially designed for freezing foods to prevent freezer burn and ensure maximum freshness. (ehow.com)
Flavor1
  • The extraordinary flavor of Goya's frozen fruit pulps will surprise you. (goya.com)
Portions2
  • These rösti (similar to latkes or hash browns) come in pancake-size portions and are baked (or fried) from frozen. (todaysparent.com)
  • Of course if you've cooked any previously raw, frozen foods, you can refreeze any portions you don't eat. (nrdc.org)
Approaches2
  • In the first of this series, as a high-level overview, we explore different methodologies and approaches to tracing, freezing and recovering cryptocurrency in Hong Kong, Singapore and other common law jurisdictions. (reedsmith.com)
  • Freezing and snow levels can also rise as warm air floods a region, particularly as a warm front approaches. (rei.com)
Tend1
  • While it is possible to freeze cabbage, its structure does tend to break down on freezing. (wikihow.com)
Spots2
  • Here are a few of our favorite spots serving frozen rosé, and a DIY tip from each. (robbreport.com)
  • Just try to do what I did and see if it freezes for you at those spots. (epicgames.com)
Double freeze1
  • If you want zucchini that's easier to divide, do a double freeze. (allrecipes.com)
Advances1
  • Two years ago, the American Society for Reproductive Medicine, an association of infertility specialists, issued an optimistic report about advances in oöcyte cryopreservation-egg freezing, in common parlance. (newyorker.com)
Grocery1
  • And as an adult, I was the one reaching into the frozen vegetable case at the grocery store for the rock-solid peas, because that's all my kids would eat. (wired.com)
Bowl1
  • If he can somehow lead New England to victory in "the Froze Bowl," all the scoffers will have to shut up for at least a week. (theothermccain.com)
Fertility2
  • A unique service we are able to provide through our recipient mare herd is to determine efficacy of different semen treatments, as well as, frozen semen by actually performing fertility trials by breeding mares and determining pregnancy rates. (selectbreeders.com)
  • In the past five years, however, more refined freezing methods have been developed and made available to some women whose fertility has been compromised by chemotherapy or premature menopause. (newyorker.com)
Disney Frozen1
Olaf1
  • Get ready for more Olaf: A new short will show what happens directly after Elsa conjured him in the first Frozen . (ew.com)
Victory1
  • I considered getting the kids to eat anything green a triumph, and frozen peas were an easy, inexpensive, reliable victory. (wired.com)
Meats1
  • Don't count out frozen meats and fish. (nrdc.org)
Cabbage11
  • Can you freeze cabbage? (hgtv.com)
  • Learn all you need to know to start freezing cabbage. (hgtv.com)
  • Freezing cabbage makes it possible to have this nutrient-rich vegetable available beyond the growing season. (hgtv.com)
  • The first step in freezing cabbage is washing heads. (hgtv.com)
  • Quick-freeze cabbage individually on a cookie sheet. (hgtv.com)
  • Cabbage should freeze in 12 to 24 hours, depending on how large your wedges are. (hgtv.com)
  • You can also place blanched cabbage into individual packages prior to freezing. (hgtv.com)
  • For the most efficient freezing, don't over-pack bags, but keep cabbage pieces in a single layer. (hgtv.com)
  • For best quality, use frozen cabbage within nine to 14 months. (hgtv.com)
  • Use frozen cabbage as a stand-alone side dish, such as skillet cabbage and onions or stewed cabbage. (hgtv.com)
  • Provided you're okay with that, here is how to freeze a glut of cabbage. (wikihow.com)
Parchment1
  • Alternatively, the leaves can be arranged on a cookie sheet lined with parchment paper, then frozen, then bagged or added to a container. (wikihow.com)
Blender1
  • In a blender, put 2 C ice, 1 C chopped frozen pineapple, ½ C light coconut milk, ½ C tequila, and ¼ C triple sec. (wearenotmartha.com)
Degrees3
  • MIKE MOSS SAYS: Taylor, I hope I'm interpreting your question correctly, but the typical reference to 'freezing' usually means the temperature at which water will solidify into ice, which is 32 degrees Fahrenheit or 0 Celsius. (wral.com)
  • The freezing point of paradichlorobenzene is 127 degrees Fahrenheit at standard pressure. (reference.com)
  • If delivery will be delayed for more than 3-4 days, specimen should be frozen at -70 degrees Celsius (-94 or permanent marker). (cdc.gov)
(youtube.com)
RECIPES: Frozen Drinks
RECIPES: Frozen Drinks (indystar.com)
'Frozen' success: Not lost in...
'Frozen' success: Not lost in... (usatoday.com)
Wendy's® | Home of Fresh, Never Frozen Beef Since 1969
Wendy's® | Home of Fresh, Never Frozen Beef Since 1969 (wendys.com)
Yes, please, brain freeze
Yes, please, brain freeze (registerguard.com)
Lafayette under freezing rain advisory
Lafayette under freezing rain advisory (jconline.com)
Deep freeze for water pipes
Deep freeze for water pipes (metrowestdailynews.com)
Frozen surprise for Middletown family
Frozen surprise for Middletown family (app.com)
Women Freezing Their Eggs for Later Pregnancies - NBC4 Washington
Women Freezing Their Eggs for Later Pregnancies - NBC4 Washington (nbcwashington.com)
Michigan weather forecast: Expect frost, freeze Up North
Michigan weather forecast: Expect frost, freeze Up North (freep.com)
Fat Freezer Platinum: Freeze away stubborn fat | Indiegogo
Fat Freezer Platinum: Freeze away stubborn fat | Indiegogo (indiegogo.com)
Federal pay freeze: Trump cancels pay raise for federal workers
Federal pay freeze: Trump cancels pay raise for federal workers (usatoday.com)
Dogs left in freezing weather? Here's what Michigan law says about it
Dogs left in freezing weather? Here's what Michigan law says about it (freep.com)
Quincy Police voting tonight on wage freeze
Quincy Police voting tonight on wage freeze (patriotledger.com)
Free Pizza at Sweet Mimiz Frozen Yogurt
Free Pizza at Sweet Mimiz Frozen Yogurt (greenbaypressgazette.com)
Mercury, freezing rain expected to fall on Thanksgiving
Mercury, freezing rain expected to fall on Thanksgiving (hutchnews.com)
Dawes hosts 'Frozen' themed event
Dawes hosts 'Frozen' themed event (newarkadvocate.com)
Grands!™ Frozen Biscuits - Pillsbury.com
Grands!™ Frozen Biscuits - Pillsbury.com (pillsbury.com)
Driver uses Siri to call 911 after vehicle plunges into freezing Iowa river
Driver uses Siri to call 911 after vehicle plunges into freezing Iowa river (desmoinesregister.com)
Evansville nonprofit's account frozen weeks after controversial hire
Evansville nonprofit's account frozen weeks after controversial hire (courierpress.com)
Red Bull Frozen Rush Returning to NE - NECN
Red Bull Frozen Rush Returning to NE - NECN (necn.com)
Georgia citrus growers chilled but not frozen out
Georgia citrus growers chilled but not frozen out (savannahnow.com)
Best Chicken Pot Pie - Frozen Chicken Pot Pie
Best Chicken Pot Pie - Frozen Chicken Pot Pie (goodhousekeeping.com)
Trump plan calls for Palestinian state, settlement freeze
Trump plan calls for Palestinian state, settlement freeze (bangordailynews.com)
(youtube.com)
Order Icecapade Frozen Treats eGift Cards
Order Icecapade Frozen Treats eGift Cards (squareup.com)
Order Birdie's Frozen Drinks eGift Cards
Order Birdie's Frozen Drinks eGift Cards (squareup.com)
Menchie's Frozen Yogurt | Dublin, OH 43016
Menchie's Frozen Yogurt | Dublin, OH 43016 (experiencecolumbus.com)
Are canned, frozen, dried, or fresh fruits, veggies better?
Are canned, frozen, dried, or fresh fruits, veggies better? (news-press.com)
Louisville weather forecast: Flash freeze possible Saturday
Louisville weather forecast: Flash freeze possible Saturday (courier-journal.com)
Andy's Frozen Custard is opening in Knoxville on Kingston Pike
Andy's Frozen Custard is opening in Knoxville on Kingston Pike (knoxnews.com)
EPA freeze could gum up Holley Superfund site, other projects
EPA freeze could gum up Holley Superfund site, other projects (democratandchronicle.com)
Denver weather: No freezing temps or snow next week | FOX31 News
Denver weather: No freezing temps or snow next week | FOX31 News (kdvr.com)