A silver salt with powerful germicidal activity. It has been used topically to prevent OPHTHALMIA NEONATORUM.
Inorganic compounds that contain silver as an integral part of the molecule.
The seepage of fluids, debris, and micro-organisms between the walls of a prepared dental cavity and the restoration.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
Silver. An element with the atomic symbol Ag, atomic number 47, and atomic weight 107.87. It is a soft metal that is used medically in surgical instruments, dental prostheses, and alloys. Long-continued use of silver salts can lead to a form of poisoning known as ARGYRIA.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Measurement of the intensity and quality of fluorescence.
In glycogen or amylopectin synthesis, the enzyme that catalyzes the transfer of a segment of a 1,4-alpha-glucan chain to a primary hydroxy group in a similar glucan chain. EC 2.4.1.18.
A POU domain factor that activates neuronal cell GENETIC TRANSCRIPTION of GENES encoding NEUROFILAMENT PROTEINS, alpha internexin, and SYNAPTOSOMAL-ASSOCIATED PROTEIN 25. Mutations in the Brn-3c gene have been associated with DEAFNESS.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Provision of physical and biological barriers to the dissemination of potentially hazardous biologically active agents (bacteria, viruses, recombinant DNA, etc.). Physical containment involves the use of special equipment, facilities, and procedures to prevent the escape of the agent. Biological containment includes use of immune personnel and the selection of agents and hosts that will minimize the risk should the agent escape the containment facility.
The measure of the level of heat of a human or animal.
A 20-carbon dibenz(de,kl)anthracene that can be viewed as a naphthalene fused to a phenalene or as dinaphthalene. It is used as fluorescent lipid probe in the cytochemistry of membranes and is a polycyclic hydrocarbon pollutant in soil and water. Derivatives may be carcinogenic.
ENDOCARDIUM infection that is usually caused by STREPTOCOCCUS. Subacute infective endocarditis evolves over weeks and months with modest toxicity and rare metastatic infection.
Tree-like, highly branched, polymeric compounds. They grow three-dimensionally by the addition of shells of branched molecules to a central core. The overall globular shape and presence of cavities gives potential as drug carriers and CONTRAST AGENTS.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Facilities in which WARFARE or political prisoners are confined.
Reproductive sterilization without the consent of the patient.
A specialized field of physics and engineering involved in studying the behavior and properties of light and the technology of analyzing, generating, transmitting, and manipulating ELECTROMAGNETIC RADIATION in the visible, infrared, and ultraviolet range.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
A nontoxic radiopharmaceutical that is used in the diagnostic imaging of the renal cortex.
Electronic instruments that produce photographs or cathode-ray tube images of the gamma-ray emissions from organs containing radionuclide tracers.
A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
Exclusive legal rights or privileges applied to inventions, plants, etc.
The study of natural phenomena by observation, measurement, and experimentation.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
The preparation and analysis of samples on miniaturized devices.
Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)
Inorganic or organic compounds that contain boron as an integral part of the molecule.
Azoles of one NITROGEN and two double bonds that have aromatic chemical properties.

The optically determined size of exo/endo cycling vesicle pool correlates with the quantal content at the neuromuscular junction of Drosophila larvae. (1/15061)

According to the current theory of synaptic transmission, the amplitude of evoked synaptic potentials correlates with the number of synaptic vesicles released at the presynaptic terminals. Synaptic vesicles in presynaptic boutons constitute two distinct pools, namely, exo/endo cycling and reserve pools (). We defined the vesicles that were endocytosed and exocytosed during high K+ stimulation as the exo/endo cycling vesicle pool. To determine the role of exo/endo cycling vesicle pool in synaptic transmission, we estimated the quantal content electrophysiologically, whereas the pool size was determined optically using fluorescent dye FM1-43. We then manipulated the size of the pool with following treatments. First, to change the state of boutons of nerve terminals, motoneuronal axons were severed. With this treatment, the size of exo/endo cycling vesicle pool decreased together with the quantal content. Second, we promoted the FM1-43 uptake using cyclosporin A, which inhibits calcineurin activities and enhances endocytosis. Cyclosporin A increased the total uptake of FM1-43, but neither the size of exo/endo cycling vesicle pool nor the quantal content changed. Third, we increased the size of exo/endo cycling vesicle pool by forskolin, which enhances synaptic transmission. The forskolin treatment increased both the size of exo/endo cycling vesicle pool and the quantal content. Thus, we found that the quantal content was closely correlated with the size of exo/endo cycling vesicle pool but not necessarily with the total uptake of FM1-43 fluorescence by boutons. The results suggest that vesicles in the exo/endo cycling pool primarily participate in evoked exocytosis of vesicles.  (+info)

Single cell studies of enzymatic hydrolysis of a tetramethylrhodamine labeled triglucoside in yeast. (2/15061)

Several hundred molecules of enzyme reaction products were detected in a single spheroplast from yeast cells incubated with a tetramethylrhodamine (TMR) labeled triglucoside, alpha-d-Glc(1-->2)alpha-d-Glc(1-->3)alpha-d-Glc-O(CH2)8CONHCH2- CH2NH- COTMR. Product detection was accomplished using capillary electrophoresis and laser induced fluorescence following the introduction of a single spheroplast into the separation capillary. The in vivo enzymatic hydrolysis of the TMR-trisaccharide involves at least two enzymes, limited by processing alpha-glucosidase I, producing TMR-disaccharide, TMR-monosaccharide, and the free TMR-linking arm. Hydrolysis was reduced by preincubation of the cells with the processing enzyme inhibitor castanospermine. Confocal laser scanning microscopy studies confirmed the uptake and internalization of fluorescent substrate. This single cell analysis methodology can be applied for the in vivo assay of any enzyme with a fluorescent substrate.  (+info)

Maintenance of motility in mouse sperm permeabilized with streptolysin O. (3/15061)

One approach to studying the mechanisms governing sperm motility is to permeabilize sperm and examine the regulation of motility by manipulating the intracellular milieu of the cell. The most common method of sperm permeabilization, detergent treatment, has the disadvantage that the membranes and many proteins are extracted from the cell. To avoid this problem, we have developed a method that uses streptolysin O to create stable pores within the plasma membrane while leaving internal membranes intact. Sperm were permeabilized, preincubated, and then treated with 0.6 U/ml of streptolysin O. Permeabilization was assessed by fluorescent dye technologies and endogenous protein phosphorylation using exogenously added [gamma-32P]ATP. Streptolysin O-induced permeabilization rendered the sperm immotile, and the effect was Ca2+-dependent. When the cells were treated simultaneously with a medium containing ATP, streptolysin O-treated sperm maintained flagellar movement. These results demonstrate that the streptolysin O permeabilization model system is a useful experimental method for studying the mechanisms that regulate sperm motility since it allows the flagellar apparatus to be exposed to various exogenously added molecules.  (+info)

Quantification of tumour vasculature and hypoxia by immunohistochemical staining and HbO2 saturation measurements. (4/15061)

Despite the possibility that tumour hypoxia may limit radiotherapeutic response, the underlying mechanisms remain poorly understood. A new methodology has been developed in which information from several sophisticated techniques is combined and analysed at a microregional level. First, tumour oxygen availability is spatially defined by measuring intravascular blood oxygen saturations (HbO2) cryospectrophotometrically in frozen tumour blocks. Second, hypoxic development is quantified in adjacent sections using immunohistochemical detection of a fluorescently conjugated monoclonal antibody (ELK3-51) to a nitroheterocyclic hypoxia marker (EF5), thereby providing information relating to both the oxygen consumption rates and the effective oxygen diffusion distances. Third, a combination of fluorescent (Hoechst 33342 or DiOC7(3)) and immunohistological (PECAM-1/CD31) stains is used to define the anatomical vascular densities and the fraction of blood vessels containing flow. Using a computer-interfaced microscope stage, image analysis software and a 3-CCD colour video camera, multiple images are digitized, combined to form a photo-montage and revisited after each of the three staining protocols. By applying image registration techniques, the spatial distribution of HbO2 saturations is matched to corresponding hypoxic marker intensities in adjacent sections. This permits vascular configuration to be related to oxygen availability and allows the hypoxic marker intensities to be quantitated in situ.  (+info)

Organ-selective homing defines engraftment kinetics of murine hematopoietic stem cells and is compromised by Ex vivo expansion. (5/15061)

Hematopoietic reconstitution of ablated recipients requires that intravenously (IV) transplanted stem and progenitor cells "home" to organs that support their proliferation and differentiation. To examine the possible relationship between homing properties and subsequent engraftment potential, murine bone marrow (BM) cells were labeled with fluorescent PKH26 dye and injected into lethally irradiated hosts. PKH26(+) cells homing to marrow or spleen were then isolated by fluorescence-activated cell sorting and assayed for in vitro colony-forming cells (CFCs). Progenitors accumulated rapidly in the spleen, but declined to only 6% of input numbers after 24 hours. Although egress from this organ was accompanied by a simultaneous accumulation of CFCs in the BM (plateauing at 6% to 8% of input after 3 hours), spleen cells remained enriched in donor CFCs compared with marrow during this time. To determine whether this differential homing of clonogenic cells to the marrow and spleen influenced their contribution to short-term or long-term hematopoiesis in vivo, PKH26(+) cells were sorted from each organ 3 hours after transplantation and injected into lethally irradiated Ly-5 congenic mice. Cells that had homed initially to the spleen regenerated circulating leukocytes (20% of normal counts) approximately 2 weeks faster than cells that had homed to the marrow, or PKH26-labeled cells that had not been selected by a prior homing step. Both primary (17 weeks) and secondary (10 weeks) recipients of "spleen-homed" cells also contained approximately 50% higher numbers of CFCs per femur than recipients of "BM-homed" cells. To examine whether progenitor homing was altered upon ex vivo expansion, highly enriched Sca-1(+)c-kit+Lin- cells were cultured for 9 days in serum-free medium containing interleukin (IL)-6, IL-11, granulocyte colony-stimulating factor, stem cell factor, flk-2/flt3 ligand, and thrombopoietin. Expanded cells were then stained with PKH26 and assayed as above. Strikingly, CFCs generated in vitro exhibited a 10-fold reduction in homing capacity compared with fresh progenitors. These studies demonstrate that clonogenic cells with differential homing properties contribute variably to early and late hematopoiesis in vivo. The dramatic decline in the homing capacity of progenitors generated in vitro underscores critical qualitative changes that may compromise their biologic function and potential clinical utility, despite their efficient numerical expansion.  (+info)

Role of mitochondrial dysfunction in the Ca2+-induced decline of transmitter release at K+-depolarized motor neuron terminals. (6/15061)

The present study tested whether a Ca2+-induced disruption of mitochondrial function was responsible for the decline in miniature endplate current (MEPC) frequency that occurs with nerve-muscle preparations maintained in a 35 mM potassium propionate (35 mM KP) solution containing elevated calcium. When the 35 mM KP contained control Ca2+ (1 mM), the MEPC frequency increased and remained elevated for many hours, and the mitochondria within twitch motor neuron terminals were similar in appearance to those in unstimulated terminals. All nerve terminals accumulated FM1-43 when the dye was present for the final 6 min of a 300-min exposure to 35 mM KP with control Ca2+. In contrast, when Ca2+ was increased to 3.6 mM in the 35 mM KP solution, the MEPC frequency initially reached frequencies >350 s-1 but then gradually fell approaching frequencies <50 s-1. A progressive swelling and eventual distortion of mitochondria within the twitch motor neuron terminals occurred during prolonged exposure to 35 mM KP with elevated Ca2+. After approximately 300 min in 35 mM KP with elevated Ca2+, only 58% of the twitch terminals accumulated FM1-43. The decline in MEPC frequency in 35 mM KP with elevated Ca2+ was less when 15 mM glucose was present or when preparations were pretreated with 10 microM oligomycin and then bathed in the 35 mM KP with glucose. When glucose was present, with or without oligomycin pretreatment, a greater percentage of twitch terminals accumulated FM1-43. However, the mitochondria in these preparations were still greatly swollen and distorted. We propose that prolonged depolarization of twitch motor neuron terminals by 35 mM KP with elevated Ca2+ produced a Ca2+-induced decrease in mitochondrial ATP production. Under these conditions, the cytosolic ATP/ADP ratio was decreased thereby compromising both transmitter release and refilling of recycled synaptic vesicles. The addition of glucose stimulated glycolysis which contributed to the maintenance of required ATP levels.  (+info)

Simultaneous measurement of evoked release and [Ca2+]i in a crayfish release bouton reveals high affinity of release to Ca2+. (7/15061)

The opener neuromuscular junction of crayfish was used to determine the affinity of the putative Ca2+ receptor(s) responsible for evoked release. Evoked, asynchronous release, and steady-state intracellular Ca2+ concentration, [Ca2+]ss, were measured concomitantly in single release boutons. It was found that, as expected, asynchronous release is highly correlated with [Ca2+]ss. Surprisingly, evoked release was also found to be highly correlated with [Ca2+]ss. The quantal content (m) and the rate of asynchronous release (S) showed sigmoidal dependence on [Ca2+]ss. The slope log m/log [Ca2+]ss varied between 1.6 and 3.3; the higher slope observed at the lower [Ca2+]o. The slope log S/log [Ca2+]ss varied between 3 and 4 and was independent of [Ca2+]o. These results are consistent with the assumption that evoked release is controlled by the sum of [Ca2+]ss and the local elevation of Ca2+ concentration near the release sites resulting from Ca2+ influx through voltage-gated Ca2+ channels (Y). On the basis of the above, we were able to estimate Y. We found Y to be significantly <10 microM even for [Ca2+]o = 13.5 mM. The dissociation constant (Kd) of the Ca2+ receptor(s) associated with evoked release was calculated to be in the range of 4-5 microM. This value of Kd is similar to that found previously for asynchronous release.  (+info)

Light-induced calcium influx into retinal axons is regulated by presynaptic nicotinic acetylcholine receptor activity in vivo. (8/15061)

Visual activity is thought to be a critical factor in controlling the development of central retinal projections. Neuronal activity increases cytosolic calcium, which was hypothesized to regulate process outgrowth in neurons. We performed an in vivo imaging study in the retinotectal system of albino Xenopus laevis tadpoles with the fluorescent calcium indicator calcium green 1 dextran (CaGD) to test the role of calcium in regulating axon arbor development. We find that visual stimulus to the retina increased CaGD fluorescence intensity in retinal ganglion cell (RGC) axon arbors within the optic tectum and that branch additions to retinotectal axon arbors correlated with a local rise in calcium in the parent branch. We find three types of responses to visual stimulus, which roughly correlate with the ON, OFF, and SUSTAINED response types of RGC reported by physiological criteria. Imaging in bandscan mode indicated that patterns of calcium transients were nonuniform throughout the axons. We tested whether the increase in calcium in the retinotectal axons required synaptic activity in the retina; intraocular application of tetrodotoxin (10 microM) or nifedipine (1 and 10 microM) blocked the stimulus-induced increase in RGC axonal fluorescence. A second series of pharmacological investigations was designed to determine the mechanism of the calcium elevation in the axon terminals within the optic tectum. Injection of bis-(o-aminophenoxy)-N,N,N',N'-tetraacetic acid-AM (BAPTA-AM) (20 mM) into the tectal ventricle reduced axonal calcium levels, supporting the idea that visual stimulation increases axonal calcium. Injection of BAPTA (20 mM) into the tectal ventricle to chelate extracellular calcium also attenuated the calcium response to visual stimulation, indicating that calcium enters the axon from the extracellular medium. Caffeine (10 mM) caused a large increase in axonal calcium, indicating that intracellular stores contribute to the calcium signal. Presynaptic nicotinic acetylcholine receptors (nAChRs) may play a role in axon arbor development and the formation of the topographic retinotectal projection. Injection of nicotine (10 microM) into the tectal ventricle significantly elevated RGC axonal calcium levels, whereas application of the nAChR antagonist alphaBTX (100 nM) reduced the stimulus-evoked rise in RGC calcium fluorescence. These data suggest that light stimulus to the retina increases calcium in the axon terminal arbors through a mechanism that includes influx through nAChRs and amplification by calcium-induced calcium release from intracellular calcium stores. Such a mechanism may contribute to developmental plasticity of the retinotectal system by influencing both axon arbor elaboration and the strength of synaptic transmission.  (+info)

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TY - JOUR. T1 - Engineering ESPT pathways based on structural analysis of LSSmKate red fluorescent proteins with large stokes shift. AU - Piatkevich, Kiryl D.. AU - Malashkevich, Vladimir N.. AU - Almo, Steven C.. AU - Verkhusha, Vladislav. PY - 2010/8/11. Y1 - 2010/8/11. N2 - LSSmKate1 and LSSmKate2 are monomeric red fluorescent proteins (RFPs) with large Stokes shifts (LSSs), which allows for efficient separation of absorbance and emission maxima, as well as for excitation with conventional two-photon laser sources. These LSSmKates differ by a single amino acid substitution at position 160 and exhibit absorbance maxima around 460 nm, corresponding to a neutral DsRed-like chromophore. However, excitation at 460 nm leads to fluorescence emission above 600 nm. Structures of LSSmKate1 and LSSmKate2, determined at resolutions of 2.0 and 1.5, respectively, revealed that the predominant DsRed-chromophore configurations are cis for LSSmKate1 but trans for LSSmKate2. Crystallographic and mutagenesis ...
Nitric Oxide (NO) is a highly-reactive radical gas that can modify a variety of cellular targets in both eukaryotes and bacteria. NO is produced endogenously by a wide variety of organisms: For example, as a cell-signaling molecule in mammals and bacteria via nitric oxide synthase (NOS) enzymes, and as a product of denitrification. As such, it is of great benefit to NO researchers to be able to sensitively detect intracellular NO and stable reactive nitrogen species (RNS) derived from NO. To this end, a protocol for fluorescent detection of intracellular NO/RNS in biofilm cultures of the Gram-positive pathogen Staphylococcus aureus has been optimized using the commercially-available cell-permeable fluorescent stain 4-Amino-5-Methylamino-2,7-Difluorofluorescein Diacetate (DAF-FM diacetate). This compound diffuses into cells and intracellular cleavage by esterase enzymes liberates weakly-fluorescent DAF-FM, which reacts with NO or other specific RNS to become highly fluorescent (Kojima et al., 1999).
Fluorescent Molecular Rotors are sensors of microenvironmental restriction that become fluorescent only if their rotation is constrained. It has been suggested that the change of fluorescence intensity is caused by the restriction of intramolecular rotational relaxation about the donor-acceptor bond of the fluorophores.Examples of molecular constraint include increased dye (aggregation)1, binding to antibodies2, or being trapped in the polymerization of actin3. There are also studies of membrane fluidity in endothelial cells under fluid shear stress4 where fluorescent molecular rotors are used.1. Bhattacharyya, A. et al., Indian J. Biochem. Biophys., 32, 442-446 (1995).2. Iwaki, T. et al., Biochem., 32, 7589-7592 (1993).3. Sawada, S. et al., Anal. Biochem., 204, 110-117 (1992).4. Farkas, D.L. and Leif R.C. (ed.), Optical diagnostics of living cells III, Proc SPIE 3291, 101-112 (2000
A red-emitting fluorescent probe was developed for the sensitive and selective detection of H2S. Upon treatment with H2S, this probe exhibited a remarkable fluorescence enhancement (10 fold) with a large Stokes shift (125 nm). The detection limit of this probe was as low as 5.7 nM based on S/N = 3. The appli
We present a technique for observing single fluorophore molecules in solution. A mode-locked laser beam is focused into the solution, thereby defining a two-photon excitation volume localized in three dimensions. Molecules diffusing into and out of this volume produce fluorescence bursts, which are detected with a high signal-to-background ratio. The theoretical foundations for the technique are laid out, including the attendant fluorescence rate distribution, and agree with experimental results obtained for Rhodamine B molecules in water.. © 1995 Optical Society of America. Full Article , PDF Article ...
Studying the implication of hydrogen peroxide in biological processes in plants remains a challenge due to the current shortcomings of H2O2-responsive probes. The use of ContPY1, a new fluorescent probe, which is highly selective and sensitive for H2O2, was investigated. To validate the use of ContPY1 on plants, we have generated protocols employing cells suspensions and leaves, and measured specifically H2O2 production by plants using spectrofluorometry and microscopy. © 2013 Landes Bioscience. ...
ortho-Aminobenzoic acid (Abz) has been used as a convenient fluorescent donor group in internally quenched fluorescent peptides, which are employed as substrates for several proteolytic enzymes. As Abz is usually bound to the N-amino terminal of these peptides, it is of interest to investigate the Abz group fluorescent properties bound to different amino acids. We report in this article the optical absorption and fluorescent properties, in aqueous media, of Abz bound to the alpha-amino group of Ala, Gly, Leu, ne, Val, Pro, Phe, Arg, Glu, Met, Asn, Tyr, and Trp, with monomethyl-amidated alpha-carboxyl group, in order to explore the origin of the drastic reduction of Abz attached to N-alpha amino group of prolyl-peptides, we also examined the fluorescence properties of Abz-NHCH3, Abz-N(CH3)(2), and Abz-pyrrolidine. Molecular dynamics simulation and NMR data indicated a lack of periplanarity of the Abz-dimethylamide, which could be the origin of low fluorescence quantum yield of ...
We have engineered a mutant of HIV Reverse Transcriptase that can be fluorescently labeled by covalent attachment of the environmentally sensitive fluorophore 7-diethylamino-3-((((2-maleimidyl)ethyl)amino)carbonyl)coumarin (MDCC). The result is a polymerase that is kinetically indistinguishable from the wild-type enzyme, but provides a signal to monitor changes in enzyme structure that result from conformational changes induced by substrate binding. Using this system, we have expanded the kinetic model governing nucleotide binding to include an enzymatic isomerization following initial nucleotide binding. In doing so, we define the role of induced-fit in nucleotide specificity and mismatch discrimination. Additionally, we have characterized the kinetics governing the specificity and discrimination of several widely administered Nucleotide Reverse Transcriptase Inhibitors (NRTIs) used to combat HIV infection including 3TC (Lamivudine), FTC (Emtricitabine), and AZT (Zidovudine) for the wild-type ...
Modular fluorescent tags: clickable BODIPYs (co-supervisor Dr. Christoph Nitsche). New dyes for electron and energy transfer. Lighting up sugars - fluorescent probes for mono-saccharides.
Fluorescent Dyes , Reactive Fluorescent Dyes , HiLyte Fluor 594 hydrazide - TFA Salt; HiLyte Fluor 594, an alternative to Alexa Fluor 594 and DyLight Fluor 594, has spectral characteristics similar to those of Texas Red. The labeling performance and stability are better than those of Texas Red. It has high extinction coefficient (80,000 M-1cm-1), high fluorescence quantum yield (0.9) and low correction factor (0.17). HiLyte FluorTM 594 based Bioconjugates exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568 nm line of Ar-Kr laser and by the 594 nm line of orange He-Ne laser. Extinction Coefficient (M-1cm-1): 80,000 Fluorescence quantum yield: 0.90 Fluorescence Life Time (ns): 4.2; HiLyte Fluor594 hydrazide is a carbonyl-reactive fluorescent labeling dye. It can be used for labeling glycoproteins such as HRP.
ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHORS REQUEST.] The design, synthesis and spectroscopic properties of several long wavelength/NIR fluorescent sensors are discussed. Amongst them, the metal complex-indicator sensors are developed based on the indicator-displacement-assay (IDA) strategy; the other BODIPY derived fluorescent sensors are developed based on the indicator-spacer-receptor (ISR) strategy. For the IDA sensors, one [PBA-Zn receptor + ARS] sensor showed some unique dual wavelength patterns when different sugar analytes were introduced. The [Cu complex + Acid Blue 45] sensors have NIR fluorescent properties and displayed high affinity towards various amino acids and warfarin. For the ISR BODIPY sensors, two of the pH responsive sensors have fluorescent emission at 600nm and displayed appropriate pKas for a physiological environment. One piperazine-BODIPY sensor with a novel structure showed a turn-on NIR fluorescent emission when protonated. One ...
TY - GEN. T1 - Spray pyrolysis synthesis of particles possessing magnetic and fluorescent properties. Application of magnetic/fluorescent particles in immunoassays. AU - Dosev, D.. AU - Nichkova, M.. AU - Dumas, R.. AU - Liu, K.. AU - Kennedy, I. M.. PY - 2005. Y1 - 2005. N2 - Many types of fluorescent nanoparticles have been synthesized as alternatives to organic dyes in biochemistry. Magnetic beads also have a long history of biological applications. In this work we apply flame spray pyrolysis in order to engineer a novel type of particle that has both fluorescent and magnetic properties. The particles have magnetic cores of iron oxide and a fluorescent shell of europium - doped gadolinium oxide (Eu:Gd 2O 3). Measurements on a Vibrating Sample Magnetometer showed an overall paramagnetic response behavior of the composite particles. Fluorescence spectroscopy showed fluorescent spectra typical for the Eu ion in a Gd 2O 3 host with a narrow emission peak centered near 615 nm. Our synthesis method ...
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Fluorescent molecular imaging helped surgeons visually identify lung adenocarcinomas during pulmonary resection, according to study results.
Optimized for western blotting with fluorescent-conjugated antibodies. Invitrogen™ iBind™ Fluorescent Detection (FD) Solution Kit is intended for use with the iBind™ Flex Western Device (SLF2000) for use in infrared detection and dual wavelength semi-quantitative analysis. iBind Fluorescent Detection Solution Kit,X1 bottle,X5 buffer, 2 vials X100 additive, 1 vial ...
We present LIVE-PAINT, a new approach to super-resolution fluorescent imaging inside live cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied, unlike conventional super-resolution methods, which rely on directly fusing the biomolecule of interest to a large fluorescent protein, organic fluorophore, or oligonucleotide. LIVE-PAINT works by observing the blinking of localized fluorescence as this peptide is reversibly bound by a protein that is fused to a fluorescent protein. We have demonstrated the effectiveness of LIVE-PAINT by imaging a number of different proteins inside live S. cerevisiae. Not only is LIVE-PAINT widely applicable, easily implemented, and the modifications minimally perturbing, but we also anticipate it will extend data acquisition times compared to those previously possible with methods that involve direct fusion to a fluorescent protein. Oi et al. describe LIVE-PAINT, a new method to achieve superresolution fluorescent imaging within live cells.
There is no simple answer to this question as the quantum yield of a fluorescent dye can vary widely, depending on the dyes micro-environment. For example, the quantum yield of a dye attached to a protein may be very different from the quantum yield of the free dye. For dyes attached to a protein, the quantum yield is highly dependent on how many molecules of the dye are attached to the protein (i.e. degree of protein labeling). In general, a higher degree of protein labeling leads to a lower dye quantum yield due to fluorescence quenching via dye-to-dye interaction. For this reason, as the degree of labeling increases, fluorescence intensity of the labeled protein will eventually reach a maximum and start to decline thereafter. In fact, one of the best ways to compare the relative quantum yields of different dyes is to plot the total fluorescence of the labeled proteins as a function of degree of labeling by the dyes as we have done with CF® dyes and other commercial dyes. CF® dyes generally ...
Single-molecule detection has long relied on fluorescent labeling with high quantum-yield fluorophores. Plasmon-enhanced detection circumvents the need for labeling by allowing direct optical detection of weakly emitting and completely nonfluorescent species. This review focuses on recent advances in single molecule detection using plasmonic metal nanostructures as a sensing platform, particularly using a single particle-single molecule approach. In the past decade two mechanisms for plasmon-enhanced single-molecule detection have been demonstrated: (1) by plasmonically enhancing the emission of weakly fluorescent biomolecules, or (2) by monitoring shifts of the plasmon resonance induced by single-molecule interactions. We begin with a motivation regarding the importance of single molecule detection, and advantages plasmonic detection offers. We describe both detection mechanisms and discuss challenges and potential solutions. We finalize by highlighting the exciting possibilities in analytical ...
GelRed, GelGreen, EvaGreen - Fluorescence dyes, EtBr substitute Apoptosis and necrosis assay kitsCell biology accessory products Nucleic acid stains for microbiology Enzyme substrates Membrane and organelle stains Qubit® kits - DNA, RNA quantification Spectrophotometers a fluorometers Consumables GelRed Nucleic Acid Gel Stain, 10.000 x in water Biot41003 119 €144,30 € incl. Btw […]. ...
Our fluorophore conjugated secondary antibodies are available in a concentrated liquid formats and many are also offered in a convenient prediluted, ready-to-use format. ...
Real-time PCR was performed using the ABI 7700 Sequence detector (Applied Biosystems) as described previously (Biecker et al., 2004). A dual-labeled fluorogenic probe (labeled with a reporter dye at the 5′-end and a second dye, quenching the emission of the reporter dye, at the 3′-end) complementary to a sequence within each PCR product was added to the PCR reaction. Cleavage of the probe during elongation by the exonuclease activity of the TaqDNA polymerase separates the reporter from its quencher. Accumulation of PCR products is detected in real time by monitoring the increase in fluorescence of the reporter dye. The PCR reaction was performed in a volume of 25 μl containing 12.5 μl2× TaqMan PCR master mix (Applied Biosystems) as well as 1.2 μl (equivalent to 300 ng of total RNA) of cDNA. The concentrations of the primers and probes are given in Table 1. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) provided as ready-to-use primers (100 nM each) and probe (200 nM) by the ...
Naturally occurring lipid granules diffuse in the cytoplasm and can be used as tracers to map out the viscoelastic landscape inside living cells. Using optical trapping and single particle tracking we found that lipid granules exhibit anomalous diffusion inside human umbilical vein endothelial cells. For these cells the exact diffusional pattern of a particular granule depends on the physiological state of the cell and on the localization of the granule within the cytoplasm. Granules located close to the actin rich periphery of the cell move less than those located towards to the center of the cell or within the nucleus. Also, granules in cells which are stressed by intense laser illumination or which have attached to a surface for a long period of time move in a more restricted fashion than those within healthy cells. For granules diffusing in healthy cells, in regions away from the cell periphery, occurrences of weak ergodicity breaking are observed, similar to the recent observations inside living
A high dynamic range apparatus for separation and detection of polynucleotide fragments has a housing adapted to receive an electrophoresis gel holder containing an electrophoresis gel loaded with fluorophore-labeled samples; one or more laser diodes for providing radiation of a frequency suitable for excitation of the fluorophore which irradiates a an array of excitation/detection sites on the electrophoresis gel; an array of detectors aligned with the excitation/detection sites for collecting fluorescent emissions; and one or more components for increasing the dynamic range of the instrument by at least an order of magnitude. These components, which can be used individually or in combination include detectors that are connected to a signal processing system that modulates the period of signal integration employed so that large signals are totaled at short time intervals and smaller signals are totaled at longer time intervals; the use of a beam splitter to produces a high intensity beam of emitted
Quinoxalinones are widely exploited for their biological activities, but more rarely for their fluorescence behavior, partly due to a lack of data. Herein, we investigated the photophysical properties of selected 3-benzoylquinoxalin-2-ones and their NaBH4-reduced products, obtained by the rearrangement of be
MIDORIGreen Xtra is a new highly sensitive green fluorescent stain for a safe visualization of DNA and RNA in agarose gels. This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr). Remarkably, agarose gels stained with MIDORIGreen Xtra have a very low background fluorescence, which makes the identification of low amounts of DNA very easy. ...
A unique DNA-binding dye with features ideal for both qPCR and High Resolution Melting® (HRM) analysis*. EvaGreen® dye binds to dsDNA via a novel release-on-demand mechanism, which permits the use of a relatively high dye concentration in qPCR without PCR inhibition (Ref. 1).. A unique feature of EvaGreen® dye is its safety. DNA-binding dyes are inherently dangerous due to their potential to cause mutation. With this in mind, Biotiums scientists designed EvaGreen® dye such that it cannot cross cell membranes, thus preventing the dye from being in contact with genomic DNA in live cells. All other commercial PCR dyes enter into cells in a matter of minutes. SYBR® Green I, for example, has been shown to be environmentally more toxic than ethidium bromide, a well-known mutagen (Ref. 2). Independent labs have confirmed that EvaGreen dye is nonmutagenic, noncytotoxic and safe to aquatic life for direct disposal in the drain. For details, download the EvaGreen® Dye Safety Report.. An added ...
To overcome this issue of reduced resolution in STED imaging due to photodegradation, ITbMs team led by Principal Investigators Shigehiro Yamaguchi, a synthetic chemist and Tetsuya Higashiyama, a plant biologist have developed a new fluorescent dye, C-Naphox that has enhanced photostability relative to conventional dyes. C-Naphox has demonstrated to be extremely photoresistant with almost no degradation of fluorescence even after prolonged STED imaging in live cells.. C-Naphox (diarylmethylene-bridged naphthophosphole P-oxide) consists of an aromatic framework with an amino moiety incorporated for its electron-donating properties and phosphorus oxide for its electron-accepting properties, leading to intense fluorescence emissions.. Although the previously synthesized molecules in the Yamaguchi group have also led to high fluorescence intensity, it is the carbon-bridged structure in C-Naphox that is the key to its extremely high resistance to high intensity light, says Aiko Fukazawa, an ...
PTI RatioMaster is a monochromator-based wide-field microscope system for measuring ratio-fluorescence or fluorescence dye intensity of labeled proteins in nanomolar concentrations. Ratio-fluorescence microscopy is the ultimate tool to study dynamic events in live cells and cellular structures. Unlike conventional intensity based fluorescence microscopy, ratio-fluorescence eliminates the effects of photo-bleaching, cell path length variation, non-uniformity of dye loading, non-uniform field of view, making ratio-fluorescence the most accurate method to determine intracellular ion concentrations. More importantly, PTI RatioMaster is fast enough to provide essential dynamic information about ion concentrations and interactions with other molecules, drugs or added reagents. The PTI RatioMaster is capable of dynamic ratio fluorescence measurements on a millisecond timescale. A xenon arc lamp provides high intensity, continuous broadband illumination. Alternating excitation wavelengths are selected ...
Description DNA Dye Non-Toxic products from Biocrede represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, singl
Fluorescence microscopy is ideal for intracellular ion measurements and the most common of these measurements is intracellular calcium imaging measurements. Two of the most popular dyes for calcium imaging are Fura-2 and Indo-1. PTI EasyRatioPro is a monochromator-based wide-field microscope system for measuring ratio-fluorescence or fluorescence dye intensity of labeled proteins in nanomolar concentrations. Ratio-fluorescence microscopy is the ultimate tool to study dynamic events in live cells and cellular structures. Unlike conventional intensity based fluorescence microscopy, ratio-fluorescence eliminates the effects of photo-bleaching, cell path length variation, non-uniformity of dye loading, non-uniform field of view, making ratio-fluorescence the most accurate method to determine intracellular ion concentrations. More importantly, PTI EasyRatioPro is fast enough to provide essential dynamic information about ion concentrations and interactions with other molecules, drugs or added ...
IHC staining was visualized with a fluorescent microscope (Olympus BX51), and images were taken with a digital camera (Olympus DP30BW) using appropriate filters for different fluorophores or bright-field illumination for DAB stain. Identical images were taken for double IHC, overlaid, and pseudocolored using Olympus Software and Adobe Photoshop (Adobe Systems). Contrast and brightness were adjusted for fluorescent signals with Adobe Photoshop (Adobe Systems) for better visualization of neurons.. For Ad-iZ/EGFPf tracing from LepRb neurons in the DMH, we analyzed four mice with correct injections into the DMH and showed representative images of projection sites in the mPOA, DMH, PVN, and rRPa in Figure 4.. For FG tracing experiments, we focused our analysis on the mPOA and DMH/DHA to investigate colocalization of LepRb neurons with FG-traced neurons from the RMR/rRPa (n = 4), DMH/DHA (n = 3), or PVN (n = 2). Representative images were shown for the DMH/DHA and mPOA, and, in some cases, we ...
A robust lipophilic dye, based on the structures of the benzothiadiazole heterocycle, was shown to be a potent fluorescent stain for the selective imaging of lipid droplets (LDs) within both live and fixed human cells. Its small molecular framework, large Stokes shift, and vastly improved photostability over that of the current status quo, Nile Red, highlight its tremendous potential as a versatile chemical tool for facilitating LD imaging and research.
Dual-channel fluorometer for personal quantitation workflow. Designed to provide highly sensitive fluorescent detection when quantifying nucleic acids, the compact instrument is simple to operate. The Quantus Fluorometer is optimized with preprogrammed settings for Promega QuantiFluor™ Dye Systems to quantitate nucleic acids and offers the flexibility to create customized methods and quantitation settings for other fluorescent dyes. ...
Method for rapid-base sequencing in DNA and RNA with two-base labeling and employing fluorescent detection of single molecules at two wavelengths. Bases modified to accept fluorescent labels are used
Chromeo 505 is a bright green fluorescent dye that replaces fluorescein, FITC, Alexa 500, Dylight 505 or other dyes that are excitable at 488 nm. Chromeo 505 and its conjugates are bright, photostable and compatible with standard fluorescent microscopes.
Solon, Ohio (PRWEB) May 06, 2013 -- DNA quantitation is crucial to experimental design and interpretation of results, especially in sensitive molecular biology
Chromeo 642 is a bright dark-red fluorescent dye that replaces Cy5, Alexa 647, DyLight 649 or other dyes that are excitable from 630 nm-650 nm. Chromeo 642 and its conjugates are bright, photostable and compatible with standard fluorescent microscopes.
Contrast agents play an important role in the study of biological tissues and whole organisms, since they enable visualization of functional structures. Fluorescent contrast agents also enable specific targeting in therapeutic approaches. Developing optimized contrast agents is central to optimizing the performance of both imaging and therapy. The ideal contrast agent for optical microscopy combines high resolution, specific targeting of functional groups, 3D imaging (depth resolution), low toxicity, low bleaching (long observation time at high signal), and high signal to noise (low background). Traditional organic dyes and fluorescent proteins are excited in the ultraviolet (UV) or blue spectral region, and emit at a longer-wavelength that is Stokes-shifted. The use of the short-wavelength excitation leads to a short penetration depth of the excitation light and give rise to autofluorescence, photobleaching and photodamage to biological specimens. It is thus primarily suited to pathological and ...
A toxin is used to introduce an otherwise cell-impermeant fluorophore-antibody (or some thing which is equally specific) to bind to an intracellular protein which allows for super resolution imaging and single particle tracking inside the living cell.
Current techniques for observing the cytoskeleton can be difficult to get into living cells, can be toxic, and are usually limited in resolution and duration, since the signal wears off over time. A common technique is fluorescence microscopy, where fluorescent molecules (probes) are attached to cell structures and then lit up against a dark background.. The team of Kai Johnsson at EPFL has developed novel fluorescent probes that can easily enter live cells, are non-toxic, have long-lasting signals, and most importantly, offer unprecedented image resolution. In 2013, the researchers developed a fluorescent molecule called silicon-rhodamine (SiR), which switches on only when it binds to the charged surface of a protein like the ones found on the cytoskeleton. When SiR switches on, it emits light at far-red wavelengths.. The challenge was getting SiR to bind specifically to the cytoskeletons proteins, actin and tubulin. To achieve this, the scientists fused SiR molecules with compounds ...
In this study we employed a common high-throughput P450 enzyme assay kit to evaluate the three SCs against a specific P450 activity. The recombinant enzyme system may be more costly and less representative of physiologic conditions, but it is a more consistent platform that avoids the wide variability in enzyme expression and activity normally encountered in HLM (Snawder and Lipscomb, 2000) and hepatocytes (Rodriguez-Antona et al., 2002; Westerink and Schoonen, 2007). Also, the enzyme systems are highly specific and relatively stable, with no significant loss in activity noted after 7 hours at room temperature (Trubetskoy et al., 2005b).. Fluorescent high-throughput screening methods employ fluorescent P450 substrates that are efficiently metabolized by specific P450 isozymes to yield a product with altered fluorescent properties, usually increased fluorescent intensity (Trubetskoy et al., 2005b). The assay requires only low reactant volume to produce high signal-to-background ratio, which ...
MilliporeSigma offers a versatile range of dyes for microscopy, including high-quality Certistain® dyes, fluorescence dyes, dry dyes and dye mixtures.
Ratiometric reporters are tools to dynamically measure the relative abundance of a protein of interest. In these systems, a target protein fused to a fluorescent or bioluminescent reporter is expressed with fixed stoichiometry to a reference protein fused to a second reporter. Both fusion proteins are encoded on a single transcript but are separated during translation by a 2A
Abnormal concentrations of ATP are associated with many diseases and cancers, and quantitative detection of ATP is thus of great importance for disease diagnosis and prognosis. In the present work, we report a new dual recycling amplification sensor integrated with catalytic hairpin assembly (CHA) to achieve high sensitivity for fluorescent detection of ATP. The association of the target ATP with the aptamer beacons causes the allosteric structure switching of the aptamer beacons to expose the toehold regions, which hybridize with and unfold the fluorescently quenched hairpin signal probes (HP1) to recycle the target ATP and to trigger CHA between HP1 and the secondary hairpin probes (HP2) to form HP1/HP2 duplexes ...
We have synthesized a novel functionalized pillar[5]arene (PC5) and used it for fluorescent detection of iron ions (Fe³⁺). It displays a specificity response for iron ions over other common cations (Hg²⁺, Co²⁺, Ca²⁺, Ni²⁺, Pb²⁺, Cd²⁺, Zn²⁺, Cr³⁺, Cu²⁺, Mg²⁺ and Ag⁺) in a solution of DMSO/THF (1 : 4, v/v). Competitive cations did not show any significant changes in emission intensity and the fluore ...
Currently there are no approved biomarkers for the pre-symptomatic diagnosis of Alzheimers disease (AD). Cathepsin-D (Cat-D) is a lysosomal protease that is present at elevated levels in amyloid plaques and neurons in patients with AD and is also elevated in some cancers. We have developed a magnetic resonance imaging (MRI)/fluorescent contrast agent to detect Cat-D enzymatic activity. The purpose of this study was to investigate the cellular and tissue uptake of this MRI/fluorescent contrast agent. The agent consists of an MRI probe [DOTA-caged metal ion (Gd3+ or Tm3+)] and a fluorescent probe coupled to a cell-penetrating-peptide sequence by a Cat-D recognition site. The relaxivity of Gd3+-DOTA-CAT(cleaved) was measured in 10% heat-treated bovine serum albumin (BSA) phantoms to assess contrast efficacy at magnetic fields ranging from 0.24 mT to 9.4 T. In vitro, Tm3+-DOTA-CAT was added to neuronal SN56 cells over-expressing Cat-D and live-cell confocal microscropy was performed at 30 min. ...
TY - JOUR. T1 - Myosin V walks hand-over-hand. T2 - Single fluorophore imaging with 1.5-nm localization. AU - Yildiz, Ahmet. AU - Forkey, Joseph N.. AU - McKinney, Sean A.. AU - Ha, Taekjip. AU - Goldman, Yale E.. AU - Selvin, Paul R.. PY - 2003/6/27. Y1 - 2003/6/27. N2 - Myosin V is a dimeric molecular motor that moves processively on actin, with the center of mass moving ±37 nanometers for each adenosine triphosphate hydrolyzed. We have labeled myosin V with a single fluorophore at different positions in the light-chain domain and measured the step size with a standard deviation of ,1.5 nanometers, with 0.5-second temporal resolution, and observation times of minutes. The step size alternates between 37 + 2x nm and 37 - 2x, where x is the distance along the direction of motion between the dye and the midpoint between the two heads. These results strongly support a hand-over-hand model of motility, not an inchworm model.. AB - Myosin V is a dimeric molecular motor that moves processively on ...
Fluorescent probes are useful tools to investigate specific subcellular components in cells, tissues and organisms. A classic application of fluorescent ligands of a specific GPCR (G-protein coupled receptors) is the investigation of the receptor location, and, if their binding is reversible, it could provide pharmacological information such as affinity and proximity between interacting molecules. In cultured cell systems, methods based on fluorescence have permitted to observe the receptor cycling and the formation of oligomeric receptor complexes. In high throughput screening, fluorescent ligands represents a safer, more powerful and more versatile alternative to radioligands. [1] The introduction of the bulky fluorophore into a small molecule ligand could lead to deep modifications not only from a physicochemical point of view but also in its pharmacological properties. [2] In order to allow a correct interaction of the pharmacophore with its receptor, it is usually separated from the ...
Single molecule fluorescence measurements are used to probe the effects of GM1 in DPPC monolayers. Langmuir-Blodgett films of GM1 and DPPC were doped with ~10−8 mol% of the fluorescent lipid probe, BODIPY-PC, and transferred onto glass substrates at 23 mN/m. As shown previously, the individual orientation of each BODIPY-PC probe in the membrane can be measured using defocused polarized total internal reflection fluorescence microscopy, revealing changes in film properties at the molecular level. Here, BODIPY-PC tilt angle histograms are used to characterize the effects of GM1 in DPPC films from 0.05 mol% to 100 mol% GM1. At high GM1 levels (,5 mol% GM1), trends in the single molecule measurements agree with previous bulk measurements showing the turnover from condensing to expanding influence of GM1 at ~20 mol%, thus validating the single molecule approach. At biologically relevant, low concentrations of GM1 (,5 mol% GM1), where bulk fluorescence measurements are less informative, the single ...
TMA-DPH (1-(4-trimethylammonium)-6-phenyl-1,3,5-hexatriene), a hydrophobic fluorescent membrane probe, interacts with living cells by instantaneous incorporation into the plasma membrane, where it becomes fluorescent. It then follows the intracellular constitutive membrane traffic and acts as a bulk membrane marker of the endocytic pathway (Illinger, D., P. Poindron, P. Fonteneau, M. Modolell, and J. G. Kuhry. 1990. Biochim. Biophys. Acta. 1030:73-81; Illinger, D., P. Poindron, and J. G. Kuhry. 1991. Biol. Cell. 73:131-138). As such, TMA-DPH displays particular properties mainly due to partition between membranes and aqueous media. From these properties, original arguments can be inferred in favor of the maturation model for the endocytic pathway, against that of pre-existing compartments, in L929 cultured mouse fibroblasts. (a) TMA-DPH labeling is seen to progress from the cell periphery to perinuclear regions during endocytosis without any noticeable loss in fluorescence intensity; with a ...
购买CGP-54626A Fluorescent ligand (Red), a fluorescent GABAB antagonist。使用Abcam高品质的CGP-54626A Fluorescent ligand (Red)帮助您更快取得科研成果。
Spectral properties of carbocyanine dye 3-methyl-2-[3-methyl-2-(3-methyl-2,3-dihydro-1,3-benzothiazole-2-iliden)-1- butenyl]-1,3-benzothiazole-3-il iodide (Cyan betaiPr) in water solution, as well as in the presence of different types of double stranded DNA have been studied. While in water solution of free dye Cyan betaiPr stays mainly in monomeric form, in the presence of DNA the dye molecules form J-aggregates. The molecular structure of these J-aggregates causes the Davydov splitting of their absorption band, corresponding to the first electronic transition. A study of site-specificity showed that in the presence of poly (dA/dT) the majority of Cyan betaiPr molecules form J-aggregates, while in the presence of poly (dGC/dGC) dye molecules stay mainly in monomeric form and in presence of chicken erythrocytes DNA both J-aggregate and monomeric forms of dye are present. We suppose that Cyan betaiPr molecules aggregate in DNA groove, which serves as a template for J-aggregate forming. An ...
Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Calcium ions play an important role in cell function, acting as effectors and/or signaling molecules for a variety of cellular biochemical and physiological processes. The development of a variety of Ca2+-sensitive fluorescent indicators and advancements in imaging technology have enhanced the ability to follow both global and localized changes in intracellular Ca2+ at ever improving temporal and spatial resolution. Ratiometric Ca2+ indicators like fura-2 permit the measurement of intracellular Ca2+ concentration (Grynkiewicz et al., 1985). But, they tend to have a limited dynamic range and a significant fluorescence background, which reduce their sensitivity to small local increases in Ca2+. On the other hand, certain nonratiometric Ca2+ indicators, such as fluo-3, although not able to give a dynamic direct read-out of the Ca2+ concentration, have very low fluorescence when not bound to Ca2+ and have a significant increase in fluorescence intensity upon binding Ca2+ (e.g., ∼200 times for ...
N-Alkylation of a novel pyridine sensor results in pyridinium salts whose conformations are stabilised by pyridinium cation-π interactions resulting in a fluorescent response that can be used to sense the presence of alkylating agents in solution at low concentration.. ...
Fluorescence Dye 620-M with Streptavidin conjugate. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications. (U0293) - Products - Abnova
FDSS is a kinetic plate reader in Fluorescence and Luminescence, and suitable for reading the kinetics of the living cells which those response, calcium flux, mobilization for example, is very fast and need to monitor its kinetic property. GPCR is one of the most highly used application field for FDSS kinetic reader monitoring the calcium transient of the living cells. From the basic fluorescence dye such like Fluo-4, Fura-2 and to more various type of fluorescence dye such like FRET, and Flash Luminescence (Aequorine etc.,) reading technology can be used in our Kinetic reading platform.. FDSS7000EX is a lamp&filter base High-end system, capable of 1536 cell based assay with multiple washing ability and 2 dispenser head setup for the sticky compounds. It has more flexibility in having multiple of reading settings. Ratiometric Fura-2, Single wavelength Fluo-3 type Calcium flux, mobilization reading are possible. Some dye which requires FRET can also be done by using the motorized emission filter ...
Fam Labeled Fluorogenic Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
We have examined the migration of murine macrophages from the vascular compartment to normal and inflammatory tissues by the adoptive transfer of resident peritoneal macrophages (RPM phi) fluorescently labeled with the hydrophobic dye 1,1-dioctadecyl 3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI). After initial labeling of the plasma membrane of RPM phi, the dye accumulated stably in intracellular vesicles of low density (rho = 1.042-1.045 kg/l) and cells remained viable in culture for 4 weeks. Like the normal monocyte, DiI-RPM phi, but not exudate-derived or fixed cells, migrated to peritoneal exudates, following i.v. adoptive transfer, by a mechanism inhibitable by an antibody to the type 3 complement receptor. In the absence of an inflammatory stimulus there was no migration to the peritoneal cavity, and DiI-RPM phi accumulated within 4 h in the red pulp and marginal zone of the spleen. By day 6 these cells still formed a tight ring of fluorescence in the marginal zone alone, ...
노인성 치매에서 가장 많은 비율을 차지하는 알츠하이머병 (Alzheimers disease) 환자의 뇌에는 이 질병의 핵심 표지자로 알려져 있는 아밀로이드 단백질 (amyloid β protein)이 쌓여있으며, 이 단백질이 질병의 발달과 연관성에 대한 많은 연구가 있다. 아밀로이드 단백질 연구에 있어서 조직염색 (tissue staining)을 통한 공초점 (confocal microscope) 그리고 이광자 현미경 (two-photon microscope) 촬영이 큰 역할을 하고 있으며, 이에 아밀로이드 단백질 특이한 형광염료 (fluorescence dye)의 중요성이 부각되었다. 본 연구에서는 기존의 아밀로이드 단백질 형광염료와는 다른 새로운 형광염료를 개발하였다. 형광염료내의 전자기부자 (donor)와 수락자 (acceptor)사이의 종류 그리고 두 분자 사이의 거리를 조절함으로써 조직염색 시 더 적은 자가형광을 내며, 더 특이적으로 아밀로이드 ...
I used single molecule fluorescence and fluorescence lifetime measurements of fluorescent dyes conjugated to DNA to better understand the photophysical and photochemical interactions between organic dye molecules and DNA. This process is important, because fluorescent dyes are frequently used as labels for DNA, but interactions with specific DNA bases can significantly affect the fluorescent properties of the dyes, leading from chromatic shifts all the way to fluorescence quenching. Thus, it is very important to obtain a full understanding of the photophysics of these interactions if cDNA or siRNAs are used as molecular probes. I studied DNA hairpins that were labeled with different numbers of red-emitting Atto655 dyes. I investigated three different samples: a DNA hairpin labeled with three dyes, one with two dyes, and one with only one dye attached to specific bases and well-known spatial separations. Sample preparation consisted of denaturing and annealing the short synthesized single strands ...
The use of labelling or staining agents has greatly assisted the study of complex biological interactions in the field of biology. In particular, fluorescent labelling of biomolecules has been demonstrated as an indispensable tool in many biological studies. Types of fluorescent labelling agents that are commonly used include conventional classes of organic fluorophores such as fluorescein and cyanine dyes, as well as newer types of inorganic nanoparticles such as QDs, and novel fluorescent latex/silica nanobeads. The newer classes of fluorescent labels are gaining increasing popularity in place of their predecessors due to their better optical properties such as possessing an enhanced photostability and a larger Stokes shift over conventional organic fluorophores, for example. This paper gives an overview of the recent advances on these luminescent nanomaterials with emphases on their optical characteristics that are crucial in fluorescence microscopy, both advantages and limitations in their ...
The RNAscope® Multiplex Fluorescent assays provide the same exceptional sensitivity as our singleplex assays, allowing single-molecule detection of up to four RNA targets simultaneously. The RNAscope® Multiplex Fluorescent assays are ideal for co-localization studies of any genes in nearly any tissue type using fluorescent labels. Advanced Cell Diagnostics offers two types of multiplex fluorescent assays. RNAscope® Fluorescent Assay, our first generation assay, is an all in one kit, ideal for fresh or fixed frozen tissues.
This application note describes the use of the SPECTRAmax GEMINI XS fluorescence microplate reader, which helps in studying the changes in levels of intracellular cAMP.
Recombinant Proteins , Streptavidin and Labeled Streptavidin , Streptavidin, HiLyte Fluor 555 conjugated; HiLyte Fluor555-streptavidin conjugate has been optimized in fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised streptavidin function. The spectrum of HiLyte Fluor555 is only slightly red-shifted compared to those of Cy3 dyes, resulting in an optimal match to filters designed for Cy3 dyes. The fluorescence of HiLyte Fluor555 can be observed at excitation/emission wavelength of 554 nm /570 nm. HiLyte Fluor555 is more photostable than Cy3 , providing researchers with additional time for capturing image. HiLyte Fluor555 - protein conjugates can sustain treatments during immunofluorescent staining, fluorescence in situ hybridization, flow cytometry and other biological applications without hydrolysis.
With respect to cellular analysis, the underlying principle of flow cytometry is that a cell suspension is focused into a single cell stream, which passes through a light source (typically a laser beam). The scattered and emitted fluorescent light (if the cells are fluorescently labeled) is subsequently measured using a range of detectors and these measurements are used to generate multi-parameter data sets that describe the physical characteristics of the cells and their fluorescent properties. The size and granularity of cells can be identified on the basis of their forward and side light scatter characteristics (FSc and SSc respectively). Their characteristics and/or expression of different proteins can be further defined by pre-staining with fluorescently-labeled antibodies or molecules that identify cellular components and / or integrity (viability) or, indeed, fluorescently-labeled proteins.. Flow cytometers can evaluate cells at an extremely rapid rate (up to several tens of thousands of ...
Fluorescence in situ hybridization (FISH) is a valuable cytogenetic technique for the detection and localization of specific DNA or RNA sequences on chromosomes. Typically, this technique can be used to define spatiotemporal expression patterns of target genes. Using fluorescent DNA probes with sequences complementary to the location of the chromosome of interest, gene expression patterns can be easily detected by fluorescence microscopy and further quantitatively analyzed. In addition, improved FISH analysis also allows simultaneous observation of multiple genes by labeling different sequence fragments with different fluorophores.. In plant systems, FISH analysis has been used in a variety of crops, including wheat, rye, cucumber and melon. Based on the information on Lifeasibles official website, Lifeasibles services cover every step of the FISH assay in the plant system:. • Probe designing and construction: aside from commercially available probes, Lifeasible also provides customized ...
Fluorescein is unionised at acidic pH and the fluorescence intensity changes with pH [19, 20]. As expected, our results clearly demonstrate this effect on fluorescence levels using a FRET pair and a quencher pair with FAM. By use of the ATTO495-ATTO647N FRET pair for Hoogsteen-based parallel triplex formation, a robust and reliably LightCycler method was established. This novel FRET pair is well-suited for Tm and ΔTm determinations over a broad pH range of parallel triplex formations.. Furthermore, this FRET pair clearly demonstrates the pH independence from pH 5.5 to 7.5 of antiparallel duplex Tm determinations in contrast to the pH dependent Tm determination of parallel triplex formation from pH 4.5 to 6.0. An interesting feature of this is the negative correlation between pH and fluorescence intensity as pH increases from 4.5 to 6.0 for parallel triplex formation (Figure 3c). This is in concordance with the expected lower efficacy of parallel triplex formation due to the lack of protonated ...
Widefield fluorescent image of epithelial cells (nuclei stained with DAPI, yellow; and filamentous actin stained with Alexa Fluor 488 phalloidin, magenta )
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On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.. ©2008 Optical Society of America. Full Article , PDF Article ...
These can be very useful. Most allow you to plot out the excitation and emission curves of a number of fluorescent dyes. Some allow the user to add filter sets and excitation sources to see how well they work with the dyes being considered. Noteworthy examples include: an extensive database of single and 2-photon dye spectra assembled here at the University of Arizona (Utzinger & Boswell), Thermo-Fishers Fluorescence spectraviewer, Semrocks searchlight, Fluorophores.org, Chromas spectra viewer, Biolegends viewer, Leicas Fluoscout, Omega Opticals curvomatic, and Zeiss Fluorescence Dye and Filter Database ...
Coupling nanomaterials with biomolecular recognition events represents a new direction in nanotechnology toward the development of novel molecular diagnostic tools. Here a graphene oxide (GO)-based multicolor fluorescent DNA nanoprobe that allows rapid, sensitive, and selective detection of DNA targets in homogeneous solution by exploiting interactions between GO and DNA molecules is reported. Because of the extraordinarily high quenching efficiency of GO, the fluorescent ssDNA probe exhibits minimal background fluorescence, while strong emission is observed when it forms a double helix with the specific targets, leading to a high signal-to-background ratio. Importantly, the large planar surface of GO allows simultaneous quenching of multiple DNA probes labeled with different dyes, leading to a multicolor sensor for the detection of multiple DNA targets in the same solution. It is also demonstrated that this GO-based sensing platform is suitable for the detection of a range of analytes when ...
Single-molecule fluorescence spectroscopy and super-resolution microscopy are important elements of the ongoing technical revolution to reveal biochemical and cellular processes in unprecedented clarity and precision. Demands placed on the photophysical properties of the fluorophores are stringent a …
The rhodamine labeled f-actin did not appear in the composite image of the three different fluorophores due to the intensity of the fluorescence being too low to register. This could be due to photobleaching that had previously occurred in this specific area of the sample, or if the sample was not labeled adequately with enough fluorescent dye. Time-lapse data for all three fluorophores under the same condition revealed discrepancies in rate of decay and initial intensity for each fluorophore. A relative high initial intensity for DAPI labeled dsDNA can be explained by the relative high net local concentration of bright fluorophores. Each nucleus contains a high concentration of dsDNA, which when stained with DAPI, creates a large solid fluorescent region with overlapping fluorophores. This differs from both tubulin and f-actin, which are of tube like nature, and appear as porous regions of interest where background light can seep through and be analyzed, making the initial brightness in the ...
We recently finished our Ask the Expert discussion on Improving Live Cell Fluorescence Imaging. This week we had several interesting questions focused on combating the negative effects of imaging on cell health and viability including looking at media options as a possible solution.
Some APC-Cy7 conjugates show changes in their emission spectra with prolonged exposure to paraformaldehyde. For instruments equipped with a 561 nm Yellow-Green laser, a 585/15 nm filter is recommended for detection. When making decisions about which fluorochromes to use in your experiments, youll want to know their relative emission spectra. APC (Allophycocyanin) spectrum - APC (Allophycocyanin) is ... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 605nm. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. BV650 is a tandem fluorochrome ...
New techniques for biological optical imaging are of great interest for the detection and visualization of processes and disease in both clinical and research areas. One major advancement has been the use of far red and near infrared (NIR) light, as it has the ability to penetrate tissues deeper than other parts of the spectrum which are readily scatter and absorbed by the surroundings. In order to improve the signal to noise ratio and resolution of optical images, contrast agents are used. Fluorescent markers can be modified to attach to specific molecular targets, creating small molecular probes. These targets can be disease sites, or biological molecules which play a major role in processes such as tumor growth. It was our goal to create a new novel fluorescent probe, consisting of a cyanine based far red to NIR marker, and an n-hydroxysuccinimide (NHS) derivative to act as a linker, which could then bind with biological species containing primary amides such as proteins and antibodies, in this
The Quantus Fluorometer is a dual-channel fluorometer for your personal quantitation workflow. Designed to provide highly sensitive fluorescent detection when quantifying nucleic acids, the compact instrument is simple to operate.
A new fluorescent Zn2+ chemosensor (P1) based on a functionalized porphyrin was synthesized and characterized. P1 displayed dramatic ratiometric variations in absorption and fluorescent emission spectra upon exposure to Zn2+ due to the formation of a 1:1 Zn2+/P1 complex. The sensor also exhibited high selectivity and sensitivity toward Zn2+ over other common metal ions in the physiological pH range with a detection limit of 1.8 mM. The sensor showed fast response times and excellent reproducibility, thus confirming its potential applicability as a fluorescent sensor for Zn2+ sensing.
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to a macromolecule, serving as a marker (or dye, or tag, or reporter) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, i.e., fluorescent imaging and spectroscopy. Fluorescein, by its amine reactive isothiocyanate derivative FITC, has been one of the most popular fluorophores. From antibody labeling, the applications have spread to ...
SH2 protein microarray assays of HA4 specificity(a) Fluorescence images of SH2 chips following incubation with fluorophore-labeled HA4. Each SH2 domain is spott
TY - JOUR. T1 - Highly photostable ketopyrrolyl-BODIPYs with red aggregation-induced emission characteristics for ultrafast wash-free mitochondria-targeted bioimaging. AU - Wu, Hao. AU - Guo, Xing. AU - Yu, Changjiang. AU - Wong, Wai Yeung. AU - Hao, Erhong. AU - Jiao, Lijuan. PY - 2020/5. Y1 - 2020/5. N2 - Subcellular organelle-specific probes, including mitochondria-targeted fluorescent probes, have attracted enormous research interests because they can monitor or visualize the morphology or biological activities of specific organelles and play an indispensable role in disease diagnosis. To follow the process, highly specific and photostable fluorescent probes are in demand. However, commercially available mitochondria probes normally suffer from poor photostability under laser irradiation and aggregation caused quenching (ACQ) in the aggregate state. In this work, two simple aggregation-induced emission(AIE)-active meso-2-ketopyrrolyl BODIPYs were developed via a convenient one-pot synthetic ...
Phosphorescent molecules are attractive complements to fluorescent compounds for bioimaging. Time-gated acquisition of the long-lived phosphorescence signals provides an effective means to eliminate unwanted background noises due to short-lived autofluorescence. We have previously investigated the molecular principles governing modulation of photoinduced electron transfer in phosphorescence zinc probes that were based on biscyclometalated Ir(III) complexes (Woo, H. et al. J. Am. Chem. Soc. 2013, 135, 4771-4787). The studies established that phosphorescence turn-on responses would be attainable for Ir(III) complexes with high triplet-state energies. This sets an upper limit to an emission wavelength, restricting the development of red- or near-IR-phosphorescence turn-on probes. To address this challenge, we designed and synthesized a new phosphorescent probe having an electron-deficient 2-(2-pyridyl)pyrazine diimine ligand tethering a di(2-picolyl)amine (DPA) zinc receptor. This ligand control ...
We offer Amine-Reactive Fluorophores; Bioconjugates; Biotin/Desthiobiotin; Buffers, Detergents; Caged Probes; Cell Analysis Kits; Cell stains; Click Chemistry; Enzyme Substrates; Fluorescent Mitochondrial Probes; Fluorescent Probes; Fluorescent/Non-Fluorescent Ion and pH Indicators; Fluorescent/Non-Fluorescent Lipids, Phospholipid Probes; Gel & Membrane Stains; Kits and Assays; Monomers; Natural Products and Their Fluorescent/None-Fluorescent Conjugates; Neurotransmitter receptors Probes; Nucleotides/Nucleosides; Protein Kinase Inhibitors (PKI); Quencher/Non-Fluorescent Probes; Reactive Probes and Related Products; Sensors; Steroids and Related Products
We offer Amine-Reactive Fluorophores; Bioconjugates; Biotin/Desthiobiotin; Buffers, Detergents; Caged Probes; Cell Analysis Kits; Cell stains; Click Chemistry; Enzyme Substrates; Fluorescent Mitochondrial Probes; Fluorescent Probes; Fluorescent/Non-Fluorescent Ion and pH Indicators; Fluorescent/Non-Fluorescent Lipids, Phospholipid Probes; Gel & Membrane Stains; Kits and Assays; Monomers; Natural Products and Their Fluorescent/None-Fluorescent Conjugates; Neurotransmitter receptors Probes; Nucleotides/Nucleosides; Protein Kinase Inhibitors (PKI); Quencher/Non-Fluorescent Probes; Reactive Probes and Related Products; Sensors; Steroids and Related Products
Nonlinear microscopy. Researcher using a two-photon excitation microscope to image living tissue (images on screen at left). Different cells within the tissue have been labelled with different fluorescent dyes. An infrared laser beam is focused through the objective lens. As it hits the fluorescent dyes it excites them, causing them to fluoresce. The laser emits pulses that last for only femtoseconds (one millionth of one billionth of a second). This imaging technique is able to penetrate deep into biological tissue and image entire networks of cells. - Stock Image C019/7668
Kinetics of B-fragment transport from EE/RE to the Golgi apparatus. (A) Confocal microscopy on living HeLa cells. Fluorophore-labeled B-fragment was internalize
The strong fluorescence response (10-fold increase) after induction with IPTG shows the functionality of GFP inside the fusion protein. After the induction a TEV protease is produced which is specifically cutting the recognition sequence build inside the linker (K1319016) between GFP and K1319002. The cutting results in a separation of GFP and REACh2 resulting in a collapse of the FRET system between the two. This results in a fluorescence signal of GFP due to the fact that the emission is no longer absorbed by REACh2 and emitted as heat but rather as fluorescence with a wavelength of 511 nm. The overall fluorescence of the double plasmid system reaches the fluorescence level of the positive control indicating a total clavage of all fusion proteins by the produced TEV protease. The very low fluorescence in the non induced double plasmid system of K1319014 and K1319008 shows the functionality of K1319002. As established before, the GFP is being expressed correctly inside the fusion protein, ...
Fluorescent Dyes[edit]. Hi Shaddack, I came across a few of your articles on Fluorescernt dyes. I have a product I'm working on ... that will require that I can apply a fluorescent dye to Cyanoacrylate (super glue), so that when combined, the two materials ... We have not been able to find an existing dye that would be compatible with cynoacrylate. Can you help? ... Perhaps you can verify/correct my guesses at the spectrum of the origin of the peaks in light from fluorescent tubes?[3]-- ...
Fluorescent dye uses[edit]. Colorless fluorescent dyes that emit blue light under UV are added as optical brighteners to paper ... UV fluorescent dyes are used in many applications (for example, biochemistry and forensics). Some brands of pepper spray will ... UV fluorescent dyes that glow in the primary colors are used in paints, papers, and textiles either to enhance color under ... In some types of nondestructive testing UV stimulates fluorescent dyes to highlight defects in a broad range of materials. ...
Fluorescent dyes can act very differently. Generally, a fluorescent dye will be excited by a coherent light source (a laser) at ... However, there are many other mechanisms by which fluorescent dyes can act. Some dyes are able to diffuse across membranes. By ... If you can identify which CD is presented on your cells of interest, then you can stain your sample with a fluorescent dye ... Certain fluorescent dyes can be used to characterize kinetic intracellular activity rather than fixing cells in formaldehyde ...
In contrast to thermofluor, no external fluorescent dye is needed because the flavin cofactor is already present in the flavin- ... Materials: A fluorometer equipped with temperature control or similar instrumentation (qPCR machines); suitable fluorescent dye ... 0.5-5 μM protein with dye into a suitable assay buffer. The exact concentrations of protein and dye are defined by experimental ... A prominent advantage of this technique is that no reporter dyes need be added as tryptophan is an intrinsic part of the ...
Fluorescent dyes can be chosen to obtain light emission at different wavelengths, and compounds such as perylene, rubrene and ... fluorescent and phosphorescent dyes and conjugated dendrimers. A number of materials are used for their charge transport ... Alq3 has been used as a green emitter, electron transport material and as a host for yellow and red emitting dyes. The ... Coherent emission from a laser dye-doped tandem SM-OLED device, excited in the pulsed regime, has been demonstrated. The ...
CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration ... Parish CR, Glidden MH, Quah BJ, Warren HS (February 2009). "Use of the intracellular fluorescent dye CFSE to monitor lymphocyte ... Weston SA, Parish CR (October 1990). "New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and ... Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples ...
"PBXL Fluorescent Dyes for Ultrasensitive Direct Detection". Journal of Fluorescence. 9 (3): 197-208. doi:10.1023/A: ... Phycobilisome versus cyanine dye detection capabilities in Western blot application. Singh, NK; Sonani, RR; Rastogi, RP; ...
1-Aminopentane Demmig, Stefan; Langhals, Heinz (1988). "Very soluble and Photostable perylene Fluorescent Dyes". Chemische ...
... (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm ... Parish, Christopher (December 1999). "Fluorescent dyes for lymphocyte migration and proliferation studies". Immunology and Cell ... The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading. It can be incorporated into ...
Resch-Genger, Ute; Grabolle; Cavaliere-Jaricot; Nitschke; Nann (August 2008). "Quantum dots versus organic dyes as fluorescent ... anti-counterfeiting dyes and paints, chemical sensing, and fluorescent tagging. However, unmodified quantum dots tend to be ... CdSe QDs have been shown to possess optical properties superior to organic dyes. The ZnS shell has a two-fold effect: to ... Cell Influence and Imaging Adding iron oxide nanoparticles to the QD micelles allows them to be fluorescent and magnetic. These ...
The use of fluorescent dyes has been explored. These involve labelled proteins targeted at biomarkers, nucleic acid sequences ... The isolate is then exposed to fluoresent dye, which will be luminescent when viewed. Improvements to existing platforms are ...
Boiling a solution of Nile blue with sulfuric acid produces Nile red (Nile blue oxazone). Nile blue is a fluorescent dye. The ... "Benzophenoxazine-based fluorescent dyes for labeling biomolecules" (PDF). Tetrahedron. 62 (48): 11021. doi:10.1016/j.tet. ... These dyes aggregate in the tumor cells, especially in the lipid membranes, and/or are sequestered and concentrated in ... Nile Blue and related naphthoxazinium dyes can be prepared by acid-catalyzed condensation of either 5-(dialkylamino)-2- ...
... owing to the high extinction coefficient combined with a comparable quantum yield to fluorescent dyes) as well as their ... "Quantum dots versus organic dyes as fluorescent labels". Nature Methods. 5 (9): 763-775. doi:10.1038/nmeth.1248. PMID 18756197 ... various kinds of organic dyes are used. However, as technology advances, greater flexibility in these dyes is sought. To this ... The technology, which is assigned to the Massachusetts Institute of Technology, uses a "quantum dot dye that is delivered, in ...
The target molecules are labelled with fluorescent dyes. The fluorescent detection enables monitoring the process in real time ... The test sample is fluorescent labelled to monitor the molecular interactions. The analysis of molecular interaction patterns ... into one fragment to ensure proper quantification of the hybridization intensity It should allow coupling of multiple dyes On- ...
Hartley, BS; V Massey (1956). "The active center of chymotrypsin: 1. Labelling with a fluorescent dye". Biochimica et ... Some of the values are used to estimate the extent of success in attempts to conjugate the dye to a protein. Other values may ... or blue-green-fluorescent sulfonamide adducts. It can also be made to react with secondary amines. Dansyl chloride is widely ...
Historically, artificially dyeing fish was common. Glassfish, in particular, were often injected with fluorescent dyes. The ... The tail is cut off and dye is injected into the body. The piece also included the first documented evidence to demonstrate ... Hong Kong suppliers were offering a service in which fish could be tattooed with company logos or messages using a dye laser; ... that parrot cichlids are injected with coloured dye. ...
Fragmented nucleic acid sequences of target, labelled with fluorescent dyes. A detection system that records and interprets the ...
Sterically Protected Fluorescent Near-IR Dyes" (PDF). J. Am. Chem. Soc. 127 (10): 3288-3289. doi:10.1021/ja042404n. PMID ... Studies with cyclodextrin-protected rotaxane azo dyes established this characteristic. More reactive squaraine dyes have also ... Potential application as long-lasting dyes is based on the enhanced stability of the inner portion of the dumbbell-shaped ... The enhanced stability of rotaxane dyes is attributed to the insulating effect of the macrocycle, which is able to block ...
Localization of embryonic antigens by antisera labelled with fluorescent dyes. Nature. 1954 Dec 4;174(4440):1059. PubMed PMID ...
... the virus particles separate the fluorescent dyes used for signalling to prevent the formation of non-fluorescent dimers that ... April 2006). "Fluorescent signal amplification of carbocyanine dyes using engineered viral nanoparticles". Journal of the ...
Biological samples are often treated with fluorescent dyes to make selected objects visible. However, the actual dye ... The authors speculate about fluorescent dyes for in vivo investigations. They cite Minsky's patent, thank Steve Baer, at the ... Also, transgenic techniques can create organisms that produce their own fluorescent chimeric molecules (such as a fusion of GFP ... Virtual CLSM (Java-based) Animations and explanations on various types of microscopes including fluorescent and confocal ...
... the transfer of properties of highly fluorescent dyes via spatial and electronic isolation of the dyes by mixing cationic dyes ... "Plug-and-Play Optical Materials from Fluorescent Dyes and Macrocycles". Chem. 6 (8): 1978-1997. doi:10.1016/j.chempr.2020.06. ... Scientists report the creation of the brightest fluorescent solid optical materials so far by enabling ... "Chemists create the brightest-ever fluorescent materials". phys.org. Retrieved 6 September 2020. "Scientists create the ...
... the virus particles separate the fluorescent dyes used for signaling in order to prevent the formation of non-fluorescent ... Soc., 125, 3192 (2003). Fluorescent signal amplification of carbocyanine dyes using engineered viral nanoparticles. Carissa M. ...
Texas Red is a red fluorescent dye. Texas Red may also refer to: The 1976 LP by Red Steagall, or the title song Texas Red ( ...
... or CFW is a fluorescent blue dye that is used to bind to the polysaccharide polymers of amebic cysts. It ... Hoch, H.C.; C.D. Galvani; D.H. Szarowski; J.N. Turner (2005). "Two new fluorescent dyes applicable for visualization of fungal ... Calcofluor-White is an fluorescent dye, meaning it has an excitation and emission wavelength that differs from one another. The ... Hoch, H. C.; Galvani, C. D.; Szarowski, D. H.; Turner, J. N. (2005-05-01). "Two new fluorescent dyes applicable for ...
Hence, one photon imaging using fluorescent dyes is not very efficient. If the same dye had good two-photon absorption, then ... In particular, laser dyes were used, since these have good photostability characteristics. However, these dyes tend to have 2- ... Two-photon excitation of a fluorophore (a fluorescent molecule) leads to two-photon-excited fluorescence where the excited ... The large scaling factor is introduced in order that 2-photon absorption cross-sections of common dyes will have convenient ...
In case both primers are labeled, different fluorescent dyes are required. While several common fluorescent dyes can be used ... or labeling both primers with a different fluorescent dye. The inability to retrieve sequences from T-RFLP often leads to the ... a rhodamine-based dye), and hexachlorofluorescein (HEX), the most widely used dye is 6-FAM. The mixture of amplicons is then ... Another modification which is sometimes used is to fluorescently label the reverse primer as well using a different dye, again ...
Magal, Einat; Weisbrod, Noam; Yakirevich, Alex; Yechieli, Yoseph (2008). "The use of fluorescent dyes as tracers in highly ... The use of fluorescent dyes as tracers in highly saline groundwater. Combined evaporation and salt precipitation in homogeneous ...
Having absorbed the sunlight, the fluorescent dyes then re-radiate it. Interaction with the metallic nanoparticles turns light ... A mixture of dyes is used to capture and absorb a wide spectrum of available light. ... The physicists and chemists at GreenSun, led by Renata Reisfeld, coat the glass with metallic nanoparticles and dyes to cause ... Sunlight scatters its energy, but the dye/nanoparticle mix in the GreenSun process delivers plasmons and photons of the right ...
This is achieved by using a fluorescent dye. The dyes that are used for HRM are known as intercalating dyes and have a unique ... This is because it is suggested that the lack of accuracy may result from "dye jumping", where dye from a melted duplex may get ... To follow the transition of dsDNA (double-stranded) to ssDNA (single-stranded), intercalating dyes are employed. These dyes ... New saturating dyes such as LC Green and LC Green Plus, ResoLight, EvaGreen, Chromofy and SYTO 9 are available on the market ...
The first major class of antibiotics was the sulfa drugs, derived by German chemists originally from azo dyes. ... gene rearrangements studies and fluorescent in situ hybridization (FISH) fall within the territory of pathology. ... discovered by Paul Ehrlich in 1908 after he observed that bacteria took up toxic dyes that human cells did not. ...
Fluorescent signal strength depends on many factors such as probe labeling efficiency, the type of probe, and the type of dye. ... A range of colors in mixtures of fluorescent dyes can be detected, so each human chromosome can be identified by a ... Although there are more chromosomes than easily distinguishable fluorescent dye colors, ratios of probe mixtures can be used to ... 2016-01-01). Feng, Yi; Zhang, Lin (eds.). Stellaris® RNA Fluorescent In Situ Hybridization for the Simultaneous Detection of ...
... in the presence of a fluorescent dye CPPO causes the emission of light, as per the following scheme involving 1,2- ...
In plant science, fluorescein, and other fluorescent dyes, have been used to monitor and study plant vasculature, particularly ... Other green dyes include Oregon Green, Tokyo Green, SNAFL, and carboxynaphthofluorescein. These dyes, along with newer ... The dye is able to be taken up into the plant the same way as water and moves from the roots to the top of the plant due to a ... Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology ...
... usually by incorporating radioactivity or tagging the molecule with a fluorescent or chromogenic dye. In some cases, the ... and the pattern of hybridization is visualized on X-ray film by autoradiography in the case of a radioactive or fluorescent ...
This coloring can be accomplished by either a dye such as Coomasie Brilliant Blue G-250 dye measured at 595 nm or by an ... This can make it difficult to manage color issues if for example one or more of the printing inks is fluorescent. Where a ... a dye binding substance can be added so that it can undergo a color change and be measured.[18] It is possible to know the ... colorant contains fluorescence, a bi-spectral fluorescent spectrophotometer is used. There are two major setups for visual ...
Dye testing[edit]. A known amount of dye (or salt) per unit time is added to a flow stream. After complete mixing, the ... Fluorescent chloride sensors. *Holographic sensor. *Hydrocarbon dew point analyzer. *Hydrogen sensor. *Hydrogen sulfide sensor ... For very large flows, tracer methods may be used to deduce the flow rate from the change in concentration of a dye or ...
NiCo2O4 nanostructures as a promising alternative for NiO photocathodes in p-type dye-sensitized solar cells with high ... Boron nitride nanotubes as vehicles for intracellular delivery of fluorescent drugs and probes» Nanomedicine 11[5] (2016) 447- ... Gastrodia elata-derived heteroatom-doped carbonmaterialsfor efficient oxygenreductionelectrocatalyst and as fluorescent ...
The gold nanoparticles facilitate the formation of a silver coating on the dye-labeled regions of DNA or RNA, allowing SERS to ... Each spectrum was specific, which is advantageous over fluorescence detection; some fluorescent markers overlap and interfere ... The advantage of this technique to identify gene sequences is that several Raman dyes are commercially available, which could ... can be used to target specific DNA and RNA sequences using a combination of gold and silver nanoparticles and Raman-active dyes ...
... and can be labelled with fluorescent proteins such as green fluorescent protein or the rhodamine dye tetramethylrhodamine ... "Fluorescent staining of acetylcholine receptors in vertebrate skeletal muscle". The Journal of Physiology. 237 (2): 385-400. ...
Fluorescence-based methods include such things as direct labeling with a fluorescent dye like calcein or labeling with europium ... that becomes fluorescent when released Eu3+ binds to a chelator. Fluorescence can be measured by means of multi-well ...
These proteins can be stained with fluorescent dye labeled antibodies and detected using flow cytometry. The limit of detection ...
The UV absorption of quinine peaks around 350 nm (in UVA). Fluorescent emission peaks at around 460 nm (bright blue/cyan hue).[ ... The first synthetic organic dye, mauveine, was discovered by William Henry Perkin in 1856 while he was attempting to synthesize ... 33] Quinine is highly fluorescent (quantum yield ~0.58) in 0.1 M sulfuric acid solution.[25][26] ...
Fluorescent dyes are used to label cellular compartments for a similar purpose.[48] ... Proteins in different cellular compartments and structures tagged with green fluorescent protein (here, white) ... such as attachment of fluorescent probes to amino acid side chains.[18] These methods are useful in laboratory biochemistry and ... such as green fluorescent protein (GFP).[46] The fused protein's position within the cell can be cleanly and efficiently ...
Biological samples are often treated with fluorescent dyes to make selected objects visible. However, the actual dye ... The authors speculate about fluorescent dyes for in vivo investigations. They cite Minsky's patent, thank Steve Baer, at the ... Also, transgenic techniques can create organisms that produce their own fluorescent chimeric molecules (such as a fusion of GFP ... animations and explanations on various types of microscopes including fluorescent and confocal microscopes. (Université Paris ...
Mitotic cells can be visualized microscopically by staining them with fluorescent antibodies and dyes. ...
gamma subdivision (enterics, fluorescent pseudomonads, purple sulfur bacteria, Legionella, (some) Beggiatoa). *delta ... This means they do not retain the violet dye in the Gram staining protocol.[2] ...
... or labelled with fluorescent dyes. CN-PAGE (commonly referred to as Native PAGE) separates acidic water-soluble and membrane ... The excess dye incorporated into the gel can be removed by destaining with the same solution without the dye. The proteins are ... Anionic dyes of a known electrophoretic mobility are usually included in the sample buffer. A very common tracking dye is ... This dye is coloured at alkali and neutral pH and is a small negatively charged molecule that moves towards the anode. Being a ...
Then the dye, along with the terminal 3' blocker, is chemically removed from the DNA, allowing for the next cycle to begin. ... The fluorescent label is detached from the nucleotide upon its incorporation into the DNA strand, leaving an unmodified DNA ... Main article: Illumina dye sequencing. Solexa, now part of Illumina, was founded by Shankar Balasubramanian and David Klenerman ... Main article: Helicos single molecule fluorescent sequencing. Heliscope sequencing is a method of single-molecule sequencing ...
2009). "Near-infrared solid-state fluorescent naphthooxazine dyes attached with bulky dibutylamino and perfluoroalkenyloxy ... Bacillus subtilis stained with Nile red as a membrane dye (shown in red). This strain grows partly as cell chains, so a ... Nile red has applications in cell biology, where it can be used as a membrane dye which can be readily visualized using an ... P Greenspan; E. P. Mayer & S. D. Fowler (1985). "Nile Red, A Selective Fluorescent Stain for Intracellular Lipid Droplets". ...
One reason for the increased use of pink for girls and blue for boys was the invention of new chemical dyes, which meant that ... The United States Manual on Uniform Traffic Control Devices specifies fluorescent pink as an optional color for traffic signs ... Color shifting was a common issue with early pink LEDs, where the red, orange, or pink phosphors or dyes faded over time, ... Insulation manufactured by Owens Corning is dyed pink, with the Pink Panther as its corporate mascot. The company holds a ...
The discovery in 1856 of mauveine, the first aniline dye, was a forerunner for the development of hundreds of synthetic dyes ... Pigments, unlike fluorescent or phosphorescent substances, can only subtract wavelengths from the source light, never add new ... In some cases, a pigment can be manufactured from a dye by precipitating a soluble dye with a metallic salt. The resulting ... Produced from harvested, dried, and crushed cochineal insects, carmine could be-and still is-used in fabric dye, food dye, body ...
Staining a cell with a dye such as Giemsa stain or crystal violet allows a microscopist to describe its size, shape, internal ... For example, the use of antibodies made artificially fluorescent (fluorescently labeled antibodies) can be directed to bind to ... Almost all cells readily stain with a number of basic dyes due to the electrostatic attraction between negatively charged ... cellular molecules and the positive charge on the dye. A cell is normally transparent under a microscope, and using a stain ...
... cells grown in culture and stained with antibody to tubulin (green), antibody to Ki-67 (red) and the blue DNA binding dye ... Multiphoton fluorescence image of cultured HeLa cells with a fluorescent protein targeted to the Golgi apparatus (orange), ... Tan X, Luo S, Wang D, Su Y, Cheng T, Shi C (2011). "A NIR heptamethine Dye with intrinsic cancer targeting, imaging and ... In 2011, HeLa cells were used in tests of novel heptamethine dyes IR-808 and other analogs which are currently being explored ...
... fluorescent sea marking dye, pyrotechnical signals, a survival radio and/or radio-beacon, formerly a distress marker light ...
2008 - Osamu Shimomura, Martin Chalfie, Roger Tsien for the discovery and development of the green fluorescent protein, GFP.[11 ... 1905 - Adolf von Baeyer for work on organic dyes and hydroaromatic compounds. ...
Fluorescent dye uses. Colorless fluorescent dyes that emit blue light under UV are added as optical brighteners to paper and ... UV fluorescent dyes are used in many applications (for example, biochemistry and forensics). Some brands of pepper spray will ... UV fluorescent dyes that glow in the primary colors are used in paints, papers, and textiles either to enhance color under ... In some types of nondestructive testing UV stimulates fluorescent dyes to highlight defects in a broad range of materials. ...
The use of the fluorescent dyes is limited to measuring the free Mg2+. If the ion concentration is buffered by the cell by ... Mg2+ chelators lag behind and the major fluorescence dye for Mg2+ (mag-fura 2[59]) actually has a higher affinity for Ca2+.[60] ... By fluorescent indicatorsEdit. A number of chelators of divalent cations have different fluorescence spectra in the bound and ... The major advantages are that readings can be made from cells over relatively long periods of time, and that unlike dyes very ...
Transported radioactive substrates or labeled fluorescent dyes can be directly measured, or in an indirect set up, the ... Alternatively, the compounds are radiolabeled, fluorescent or have a fluorescent tag so that the radioactivity or fluorescence ... fluorescent dyes like rhodamine 123, or calcein) can be determined in the presence of a test drug. Calcein-AM, A highly ... Activity of multidrug transporter is reflected by the difference between the amounts of dye accumulated in the presence and the ...
Fluorescent dyes bind to calcium and allow in vitro imaging of calcium activity which correlates directly with insulin release. ...
Note: See fluorescent_dye for more definitions.). Quick definitions from WordNet (fluorescent dye). ▸ noun: a yellow dye that ... Fluorescent dyes: Wikipedia, the Free Encyclopedia [home, info] Medicine (2 matching dictionaries). *Fluorescent Dyes: Medical ... Click on the first link on a line below to go directly to a page where "fluorescent dyes" is defined. General (1 matching ... Search for fluorescent dyes on Google or Wikipedia Search completed in 0.025 seconds.. Home Reverse Dictionary Customize Browse ...
Synonyms of fluorescent dye. Find synonyms for:. Noun. 1. fluorescein, fluoresceine, fluorescent dye, resorcinolphthalein, dye ... usage: a yellow dye that is visible even when highly diluted; used as an absorption indicator when silver nitrate solution is ...
A new range of rhodamine-based fluorescent dyes, known as Janelia Fluor® Dyes has been recently developed by Luke Lavis lab at ... Fluorescent Dyes for Fluorescence Imaging. It is important to note that the precise labeling of biomolecules, using bright ... Developing Fluorescent Dyes for Use in Super-Resolution Microscopy. *Download PDF Copy ... 2020, May 16). Developing Fluorescent Dyes for Use in Super-Resolution Microscopy. News-Medical. Retrieved on April 17, 2021 ...
fluorescent dye: Vocabulary.com [home, info] *fluorescent dye: Dictionary.com [home, info] *Fluorescent dye: Wikipedia, the ... fluorescent dye: Free Dictionary [home, info] *fluorescent dye: Mnemonic Dictionary [home, info] *fluorescent dye: WordNet 1.7 ... Note: See fluorescent_dyes for more definitions.). Quick definitions from WordNet (fluorescent dye). ▸ noun: a yellow dye that ... fluorescent dye: Encyclopedia [home, info] Medicine (1 matching dictionary). *fluorescent dye: Medical dictionary [home, info] ...
Two new fluorescent dyes attracted to cancer cells may help neurosurgeons more accurately localize and completely resect brain ... Green and Near-Infrared Fluorescent Dyes Make Cancer Cells Glow... CLR1501 and CLR1502 are synthetic analogs of the tumor- ... Fluorescent dyes light up brain cancer cells, reports Neurosurgery New agents show promise for fluorescence-guided tumor ... Used during surgery for brain cancer, these fluorescent dyes could help neurosurgeons to locate the tumor and to resect it as ...
Fluorescent dye-coated nanoparticles could be used to target and turn off disease-related genes. To test this concept, a team ... Fluorescent Dyes Target Disease Genes. EuroPhotonics. Mar 2015 JENA, Germany - Fluorescent dye-coated nanoparticles could be ... The biodegradable polymer nanoparticles were covered with near-infrared fluorescent polymethine dyes that made it possible to ... But the dyes also play another key function: Because they mimic a cellular transporter of liver epithelial cells, the liver ...
Not all fluorescent calcium indicators can be measured in a standard confocal microscope. An UV laser is not usually installed ... In general, any fluorescent microscope with the required excitation/emission lasers/filters can be adjusted for measuring ... Different brands and models of microscope offer different settings, so choose a fluorescent calcium indicator that suits yours ...
... fluorescent dye; wavelength effective; single fluorescent; fluorescence emissions; specific chromosomes; fluorescent emission; ... Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different ... chromosome; characterization; single; fluorescent; dye; chromosomes; characterized; emissions; excited; wavelengths; mixture; ... The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or ...
Swager, who is head of the chemistry department, and colleagues developed the new dye, called NIAD-4, through a targeted design ... To make the technique truly noninvasive, scientists must further refine the dye so it fluoresces at a slightly longer ... But now MIT scientists have a promising injectable dye that could light up amyloid plaques characteristic of the disease:. ...
Atto labels represent a comprehensive series of fluorescent labels for biomolecules, which are designed for highest sensitivity ... The Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies:. *Enhanced ... With the extensive selection available, Atto dyes can replace commonly used fluorescent dyes. There are Atto dyes suitable for ... Atto Dye Conjugates. Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for ...
ATTO-TEC® has developed the second generation of fluorescent dyes which are stable at room temperature for more than six months ... With Atto 520, Atto 565 and Atto 590 we are pleased to offer three stable fluorescent dyes as amine-reactive succinimidyl ... ATTO-TEC® Stable activated fluorescent dyes at room temperature (RT). 19.10.2001 ... Further stable activated fluorescent probes including Atto 610, Atto 655 and Atto 680 will be also available shortly. ...
Controlling the excited electronic states in luminescent systems remains a challenge in the development of fluorescent and ... phosphorescent dyes. Now, scientists in Japan have developed a unique org ... ... Smart Fluorescent Dyes. Smart Fluorescent Dyes. Modulating photo- and electroluminescence in a stimuli-responsive molecular dye ... excited electronic states in luminescent systems remains a challenge in the development of fluorescent and phosphorescent dyes ...
... as a fluorescent dye for a linear hydrolysis probe used in qPCR.... ... This is the first report describing the possibility of using a green fluorescent protein chromophore synthetic analog, P-HOBDI- ... as a fluorescent dye for a linear hydrolysis probe used in qPCR. The study was carried out on a system for detection of the ... It was demonstrated that a synthetic dye based on the P-HOBDI-BF2 chromophore can be used for labeling hydrolysis probes for ...
Looking for MAGNAFLUX Fluorescent Dye Penetrant Kit (52YX12)? Graingers got your back. Price:$2332.00. Easy ordering & ... Magnaflux® Fluorescent Dye Penetrant Kit comes with dyes, a UV light, and other accessories to help you find the tiniest cracks ... Highly sensitive fluorescent dyes comply with most military specifications for fluorescent inspection. ...
Select 2017 high quality Fluorescent Dyes products in best price from certified Chinese Chemicals manufacturers, China Dyes ... Direct Dyes Wax Dyes Acid Fluorescent Dyes Fluorescent Disperse Dyes Fluorescent Dyes Factory Wholesale Fluorescent Dyes More ... Solvent Dyes Manufacturers Direct Dyes Suppliers Wax Dyes Suppliers Acid Fluorescent Dyes Suppliers Fluorescent Disperse Dyes ... Fluorescent Acrylic Sheet Shoe Polish Dyes Fluorescent Whitening Agent Naphthol Dyes Reactive Black Dyes Fluorescent Whitening ...
... June 9, 2008 , APPEARED IN VOLUME 86, ISSUE 23 ... Until now, there hasnt been a general fluorescent dye that scientists could use to stain the material secreted by cells and ... The researchers used the dye to stain fruit fly tissue as well as bovine collagen, finding that the dye is compatible with ... Current dyeing methods, including antibody-targeted staining, have been limited to visualizing one component or only certain ...
In vivo tracking of bone marrow fibroblasts with fluorescent carbocyanine dye.. Ferrari A1, Hannouche D, Oudina K, Bourguignon ... We used a fluorescent lipophilic probe, CM-Dil, that avidly binds to the cell membrane. Human bone marrow stromal fibroblasts ...
Full spectral range of DyLight Dyes as NHS ester-activated reagents to label antibodies (protein amines) as probes for ... NHS ester activated proprietary blue fluorescent dye. Sufficient For. 5 uses to label a total of 3mg of IgG using typical ... Thermo Scientific™ Amine-Reactive DyLight Dyes are NHS ester-activated DyLight Fluors for fluorescent labeling of antibodies ... The DyLight Dyes exhibit higher fluorescence intensity and photostability than Alexa™, CyDye™ and LI-COR™ Dyes in many ...
This fluorescent tracer dye is for traditional coolant systems and pinpoints the exact location of coolant fluid leaks. Locate ... Can I use fluorescent tracer dye for leak detection?. *Yes, tracer dye is a great tool for leak detection. The dye will be ... ACDelco Coolant System Fluorescent Tracer Dye is a fluorescent leak-detection dye for traditional coolant systems. This ... ACDelco Coolant System Fluorescent Tracer Dye can be used with DEX-COOL coolants, but it may change the color of the coolants. ...
... researchers have developed a way to adjust the properties of fluorescent dyes deliberately, resultin ... The team developed the Janelia Fluor (JF) series of dyes - fluorescent molecules up to 50 times brighter than other dyes and ... Broader Palette of Fluorescent Dyes Could Advance Biological Imaging. Photonics.com. Oct 2017 ASHBURN, Va., Oct. 19, 2017 - To ... For their work, researchers at Howard Hughes Medical Institute (HHMI) focused on rhodamines, a family of fluorescent dyes that ...
"Having two color sources allows researchers to tag the sample with two dyes," said Sands. "One dye serves as a control to ... When light within a certain wavelength range hits a specific dye, the dye becomes "excited" and emits its own light, usually at ... Engineers create lab-on-a-chip using fluorescent dye to detect toxins ... For example, a dye that is excited by blue light at about 490 nanometers emits a green light in a range of 510-530 nanometers. ...
Angewandte Chemie »Chemie »STED microscopy »Yamaguchi »biological systems »dye »fluorescence »fluorescent »fluorescent dyes » ... photodegradation of fluorescent dyes becomes a serious issue. Representative photoresistant fluorescent dyes such as Alexa ... a plant biologist have developed a new fluorescent dye, C-Naphox that has enhanced photostability relative to conventional dyes ... A new photostable fluorescent dye for super resolution microscopy could serve as a powerful tool to visualize biological events ...
... a cell-permeable fluorescent dye for determining cell viability. Join researchers using high quality Calcein AM, fluorescent ... Cell-permeable fluorescent dye for determining cell viability. Hydrolyzes to green-fluorescent calcein on contact with ...
Creating a New Fluorescent Dye. Fluorescent chromophores are compounds that can emit light under certain circumstances. They ... A new highly fluorescent and symmetric pyrrole-BF2 chromophore: BOPHY. Journal of the American Chemical Society 136 (15) (APR ... BOPHY is important because it can be produced with a simple two-step procedure, and it is highly fluorescent. ... discuss a new fluorescent chromophore, or fluorophore, called BOPHY. (BOPHY stands for bis(difluoroboron)1,2-bis(1H-pyrrol-2-yl ...
Uvitex 2B fluorescent dye will bind to the chitin in fungal walls and is highly selective for fungi and algae in tissue ... A cationic fluorescent dye which specifically binds to adenine-thymine-rich DNA. Applications include detection of nanogram ... Dye can be melted or dissolved in solvent then be used for application into small injection sites.. 1 mg of solid dye can be ... 1cm² nylon filter coated with the lipophilic green emitting dye, NeuroVue® Jade.. Typical dye loading: 22-30 nmoles/mm,sup>2. ...
Previously reported methods were used to condense the two dye halves. The purified chloro-dyes (TO-Cl-1, TO-Cl-2 and TO-Cl-3) ... We have synthesized fluorescent DNA duplexes featuring multiple thiazole orange (TO) intercalating dyes covalently attached to ... Fluorescent DNA nanotags featuring covalently attached intercalating dyes: synthesis, antibody conjugation, and intracellular ... Fluorescent DNA Nanotags Featuring Covalently Attached Intercalating Dyes: Synthesis, Antibody Conjugation and Intracellular ...
Intravitreal injection of a fluorescent dye, Procion yellow, results in the complete and systematic staining of a cone ... Staining of blue-sensitive cones of the macaque retina by a fluorescent dye Science. 1981 Sep 11;213(4513):1278-81. doi: ... Intravitreal injection of a fluorescent dye, Procion yellow, results in the complete and systematic staining of a cone ...
Use maleimide chemistry to attach infrared fluorescent dyes to free sulfhydryl groups on biomolecules. Several maleimide dye ... These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein ... LI-COR IRDye® infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared ( ... If you are interested in commercial development rights using IRDye infrared dyes, please contact Business Development. ...
2-PackPowder that turns snow or water into a bright fluorescent green signal. You can write in the snow or on the ground. ... Fluorescent Dye Marker - 2-Pack. Powder that turns snow or water into a bright fluorescent green signal. You can write in the ... Orion Fluorescent Dye Marker - 2-Pack. Rating Required Select Rating. 1 star (worst). 2 stars. 3 stars (average). 4 stars. 5 ...
CaseETC PC blacklight and UV fluorescent coolant dye. Review date: 28 June 2001.. Last modified 03-Dec-2011. .. ... The dye fluoresces under ultraviolet light, which is what the blacklight emits. Put a few drops of the dye into your coolant - ... Well, the CaseETC coolant dye is The Business. If you need water dye that glows in UV light, they have it. And it is powerful ... The dye does not appear to be as glow-y when its soaked into fabric as when its floating free in water. ...
  • Tocris Bioscience is your trusted supplier of high-performance life science reagents, including receptor agonists & antagonists, enzyme inhibitors, ion channel modulators, fluorescent probes & dyes, and compound libraries. (news-medical.net)
  • A novel optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described. (osti.gov)
  • Atto dyes can be used to conjugate probes and biomolecules for multiplex applications . (sigmaaldrich.com)
  • Further stable activated fluorescent probes including Atto 610, Atto 655 and Atto 680 will be also available shortly. (innovations-report.com)
  • It was demonstrated that a synthetic dye based on the P-HOBDI-BF 2 chromophore can be used for labeling hydrolysis probes for qPCR, but fluorescence increase levels for P-HOBDI-BF 2 -labeled probes were slightly lower than those for 6-FAM-labeled ones. (springer.com)
  • Fluorescent dyes and probes are key components in multiphoton based fluorescence microscopy imaging of biological samples. (spie.org)
  • They have been functionalized with moieties to act, e.g., as efficient amine-reactive fluorescent probes for the covalent attachment onto, e.g., proteins and antibodies. (spie.org)
  • Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes. (fishersci.ca)
  • Thermo Scientific™ Amine-Reactive DyLight Dyes are NHS ester-activated DyLight Fluors for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods. (fishersci.ca)
  • I have been working on the synthesis of molecular frameworks containing specific elements since 2006, but it was since the start of ITbM in 2013 that we really started to focus on synthesizing molecules as fluorescent probes for live cell imaging," she continues. (innovations-report.com)
  • Maleimide chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. (licor.com)
  • This panel features a wide range of fluorescent probes specific to various disease areas as well as a fluorescent labeling kit to tag your own proteins, peptides or antibodies. (perkinelmer.com)
  • A distinctive combination of reagents comprised of exceptional fluorescent molecular probes including CELLESTIAL® dyes for live, fixed and permeabilized cells. (enzolifesciences.com)
  • Compatibility with the cells, with other probes in the same experiment, and with the treatments and protocols you are using can lead to the question: How do I choose one fluorescent probe over another? (thermofisher.com)
  • Authored by our R&D scientists Jason Kilgore and Nick Dolman, who have developed and tested many Molecular Probes® organelle stains, as well as imaging guru Michael Davidson of Florida State University, this series pulls together a wealth of references and personal knowledge that covers the most common fluorescent reagents for nearly every cellular structure or organelle, focusing primarily on microscopic analysis. (thermofisher.com)
  • One tool we found to be especially useful is the SYTO vital nucleic acid dyes from Molecular Probes. (wormbook.org)
  • Fluorescent probes enable researchers to detect particular components of complex biomolecular assemblies (including live cells) with exquisite sensitivity and selectivity. (biomol.com)
  • Amine-reactive fluorescent probes are widely used to modify peptides, proteins, oligonucleotides, nucleic acids, ligands and other biomolecules. (biomol.com)
  • Intercalating Dyes or Fluorescent Probes For RT-qPCR? (bitesizebio.com)
  • However, the two major categories to choose from are fluorescent intercalating dyes and fluorescent oligonucleotide probes. (bitesizebio.com)
  • Lighting up sugars - fluorescent probes for mono-saccharides. (edu.au)
  • Both PALM and dSTORM are single molecule imaging techniques involving the sequencing of individual fluorescent molecules and their repeated activation and deactivation using light of specific wavelengths. (news-medical.net)
  • Diode laser excitation at 635 nm and red-absorbing fluorescent dyes were shown to reduce autofluorescence of biological samples sufficiently so that individual antigen and antibody molecules could be detected in human serum samples. (sigmaaldrich.com)
  • The team developed the Janelia Fluor (JF) series of dyes - fluorescent molecules up to 50 times brighter than other dyes and offering more photostability as well. (photonics.com)
  • These dyes are a complete game-changer," said Ethan Garner, a bacterial cell biologist at Harvard University who has used the dyes to trace the path of single molecules in his lab. (photonics.com)
  • If molecules of a specific substance - such as an avian flu virus or anthrax - are present in the sample, the dye tags them. (berkeley.edu)
  • In order to attain an optimal fluorescent quantum yield, about 200 to 300 dye molecules can be incorporated per liposome. (uni-muenchen.de)
  • In a new study, published online in the journal Angewandte Chemie International Edition, a team of chemists and biologists at the Institute of Transformative Bio-Molecules (ITbM), Nagoya University has developed a new fluorescent dye, "C-Naphox" with enhanced photostability to enable continuous live cell imaging by STED microscopy, which opens doors to observe real-time biological events for extended time periods with high resolution. (innovations-report.com)
  • Nonetheless, the light intensity required for super-resolution fluorescence microscopy is much higher than conventional microscopy, which leads to photodegradation of fluorescent dye molecules. (innovations-report.com)
  • IRDye maleimides provide the functionality for labeling molecules that contain free sulfhydryl (-SH) groups with IRDye infrared dyes. (licor.com)
  • To achieve maximum glucose targeting and enhanced tumor uptake, the probe has been designed to contain four 2-DG molecules per dye molecule. (perkinelmer.com)
  • This is explained here as the result of magnetic field dependent triplet-triplet annihilation process, in which the triplet excitons trapped on the dye molecules play the most important role. (ebscohost.com)
  • Nanoparticles coupled to a fluorescent dye can be used to target tumor-specific molecules in breast cancer , providing a way to track the particles by NIR spectroscopy , to enhance magnetic resonance imaging (MRI) and to deliver an anticancer payload only to diseased cells. (bioopticsworld.com)
  • We propose a new, to our knowledge, monolithic multilayer optical storage medium in which data may be stored through the diffusional redistribution of fluorescent molecules within a polymer host. (osapublishing.org)
  • We believe that as fluorescent molecules bond to the polymer matrix they become more highly concentrated in the polymerized regions, resulting in the modulated data pattern. (osapublishing.org)
  • Tandem dyes are composed of two covalently attached fluorescent molecules. (biotium.com)
  • With their high density of molecules, dye nano- and microcrystals absorb strongly in the ultraviolet and the visible. (coursehero.com)
  • Since fluorescent dye molecules with orientations most closely aligned with the laser light's polarization will appear brightest, measuring the brightness of each molecule in each camera frame allowed the researchers to quantify orientation and rotational dynamics on a molecule-by-molecule basis. (thefreedictionary.com)
  • Activated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), fluorescence resonance energy transfer (FRET) techniques, receptor binding assays, and enzyme assays. (sigmaaldrich.com)
  • Proteins labeled with pyrylium dyes maintain their native charge and isoelectric points, which results in sharp and characteristic bands during electrophoresis. (activemotif.com)
  • Pyrylium dyes change their color and fluorescent properties upon reacting with proteins. (activemotif.com)
  • Due to the unique conjugation mechanism, proteins labeled with Py-Dyes maintain their native charge and isoelectric point. (activemotif.com)
  • Most of our fluorescent dyes are available in both succinimide ester and maleimide chemistries for convenient labeling of proteins. (perkinelmer.com)
  • In addition to click chemistry reagents, Chromeo Dyes are offered in a variety of formats like amine reactive NHS-Esters, which have been shown to label amines in peptides, proteins or amino modified DNA. (activemotif.com)
  • These include isotype controls as well as biotin and fluorescent conjugates for use in evaluating intracellular and extracellular proteins. (enzolifesciences.com)
  • Fluorescent indicators for Ca.sup.2+ based on green fluorescent proteins and calmodulin," Nature, vol. 388, Aug. 28, 1997, pp. 882-887. (patentgenius.com)
  • In Part I the authors focus on the BacMam reagents, which have been used for transient transduction of fluorescent proteins targeted to specific cellular structures. (thermofisher.com)
  • Localization is achieved by noncovalent, electrostatic and hydrophobic binding of dye to proteins, with signal being detected at 610 nm. (spie.org)
  • Since proteins are not covalently modified by the dye, compatibility with downstream microchemical characterization techniques such as matrix-assisted laser desorption/ionization-mass spectrometry is assured. (spie.org)
  • He does by taking slices of mouse brains and exposing the issue to misfolded proteins, and then he adds fluorescent dye. (iowapublicradio.org)
  • Pro-Q Emerald 300 glycoprotein stain generates a bright-green fluorescent signal upon reacting with periodic acid-oxidized carbohydrate groups on proteins. (springer.com)
  • With this dye it is possible to detect proteins directly in the gel without the need to transfer them to a membrane. (springer.com)
  • By taking advantage of this property of the dye, users can characterize intracellular activity as well as surface-expression of proteins. (wikipedia.org)
  • These techniques are limited by their requirement for both highly purified proteins and solvatochromic dyes, prompting the need for more robust high-throughput technologies that can be used with crude protein samples. (wikipedia.org)
  • This need was met with the development of a new high-throughput technology for the quantitative determination of protein stability and ligand binding by differential scanning fluorimetry of proteins tagged with green fluorescent protein (GFP). (wikipedia.org)
  • In general, any fluorescent microscope with the required excitation/emission lasers/filters can be adjusted for measuring intracellular calcium, but advantages and restrictions of each equip have to be considered for experimental setup. (embl.de)
  • Selection of two Atto dyes with separated emission signals supports multiple excitation and measurement results from a single experiment. (sigmaaldrich.com)
  • One major difficulty is that only a small amount of the incoming light actually results in excitation and subsequent dye emission," said Chediak. (berkeley.edu)
  • The emission color can be changed to orange or red by addition of energy-accepting Cy3 or Cy5 dyes attached covalently to the DNA duplex. (nih.gov)
  • Fluorescence emission spectra recorded for ODN3 duplex in the absence or presence of Cy5 energy acceptor dye on complementary strand. (nih.gov)
  • The shift of the absorption & emission bands together with the increased fluorescence quantum yield effectively eliminates background from any unbound dye. (activemotif.com)
  • When a short wavelength laser is used for exciting the organic violet fluorescent compound (I), a fluorescence having an emission wavelength larger than 500 nm is induced, and a reading signal can be provided by detecting the intensity of the fluorescence radiation. (freepatentsonline.com)
  • The selective binding of the bisBenizimide H33258 dye to A-T base pairs results in significant fluorescent emission at 460 nm in the presence of DNA. (prweb.com)
  • EQ quencher dyes are suitable as acceptors in fluorescence resonance energy transfer (FRET) applications due to their broad visible absorption but no detectable fluorescence emission. (enzolifesciences.com)
  • Not only must the excitation and emission spectra of the reagent match your imaging instrument, but often you also need to choose from among a number of reagents-including organic dyes, drug conjugates, and fluorescent protein constructs-that target the same structure. (thermofisher.com)
  • The dyes come in a range of excitation and emission maxima and therefore should be amenable to a variety of applications. (wormbook.org)
  • Aggregation-induced emission (AIE) dye-based fluorescent organic nanoparticles (FONs) were prepared via Schiff base interactions between an AIE dye and a carbohydrate polymer (carboxymethyl chitosan). (rsc.org)
  • Sulforhodamine 101 (SR101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). (medchemexpress.com)
  • Using long-wavelength activated Atto dyes with the appropriate excitation wavelength reduces autofluorescence due to sample, solvent, glass, or polymer support. (sigmaaldrich.com)
  • There are Atto dyes suitable for use with any common excitation light source. (sigmaaldrich.com)
  • It also allowed the team to establish principles for fine-tuning the properties of fluorophores and to develop a palette of new fluorescent and fluorogenic labels with excitation ranging from blue to the far-red. (photonics.com)
  • The fluorescent dye of the present invention comprises an organic violet fluorescent compound having a chemical structure (I) is suitable for using a short wavelength laser having a wavelength less than 500 nm as an excitation source. (freepatentsonline.com)
  • For broad compatibility with imaging platforms, an optimized fluorescent dye developed for proteomics applications should be designed taking into account that laser scanners use visible light excitation and that charge-coupled device camera systems and gas discharge transilluminators rely upon UV excitation. (spie.org)
  • The intensity of each module can be adjusted independently, precisely and reproducibly in percentage steps, so that for every fluorescent dye , the output emitted is precisely that which is needed to achieve the best possible compromise between the required excitation intensity and maximum sample protection. (thefreedictionary.com)
  • Immunoblot detection of Protein 1 and Protein 2 using two primary antibodies and two anti-IgG-Atto dye conjugates. (sigmaaldrich.com)
  • They are available conjugated to biotin or streptavidin and are offered as optimized Fluorescent Secondary Antibody Conjugates for enhanced detection. (activemotif.com)
  • They also discuss options for vesicular organelles such as endosomes (featuring pHrodo™ dextran conjugates), lysosomes (featuring the organic dye LysoTracker® Red DND-99), peroxisomes (featuring the BacMam reagent CellLight® Peroxisome-GFP, Figure 1 ), and autophagosomes (featuring another BacMam reagent, the Premo™ Autophagy Sensor GFP-LC3B). (thermofisher.com)
  • In Part III the authors describe reagents for actin (featuring Alexa Fluor® phalloidin peptide conjugates, Figure 2), tubulin (featuring the drug conjugate Tubulin Tracker™ Green), cellular membranes (featuring plasma membrane labeling with fluorescent wheat germ agglutinin (WGA) conjugates, Figure 3 ), and whole-cell and cytoplasm reagents (featuring the organic dye CFSE, also called CFDA, SE). (thermofisher.com)
  • In these applications, the stability of the chemical bond between the amine-reactive dye and biomolecule is particularly important because the fluorescent conjugates are often subjected to rigorous incubation, hybridization and washing steps. (biomol.com)
  • A number of fluorescent amino-reactive dyes have been developed to label various biomolecules, and the resultant conjugates are widely used in biological applications. (biomol.com)
  • Although FITC (fluorescein isothiocyanate), one of the most popular fluorescent labeling dyes, is predominantly used for preparing a variety of fluorescent bioconjugates, the low conjugation efficiency of FITC and the short life time of its conjugates are still troublesome for some critical biological applications. (biomol.com)
  • Mix-n-Stain™ Fluorescent Protein & Tandem Dye Antibody Labeling Kits utilize a revolutionary antibody labeling technology that allows you to easily label your antibody with APC, R-PE, PerCP or tandem dyes (phycobiliprotein/small dye conjugates) in about 4 hours. (biotium.com)
  • AAT Bioquest offers all the popular amine-reactive fluorescent dyes for peptide/protein labelings, nucleotide modifications and microarray applications. (biomol.com)
  • Neither the fluorescent properties nor the stability of these liposomes are influenced by the additional incorporation of various ara C-derivatives and lipophilic anchor groups which subsequently enable the coupling of antibodies to the liposomes. (uni-muenchen.de)
  • We labeled live neuron terminals with fluorescent primary antibodies to HRP, and also visualized the perisynaptic space with fluorescent Dextrans. (jove.com)
  • CytoTell dyes can be readily used for the multicolor applications with either GFP cells or FITC-labeled antibodies. (jimmunol.org)
  • Combined with traditional fluorochromes, such as FITC, PE, PerCP, and APC, the Vio Dyes expand Miltenyi Biotec's antibody portfolio and allow a greater selection of antibodies for multiparameter cell analysis. (miltenyibiotec.com)
  • This study was designed to load different antibodies (Abs) and a fluorescent dye onto the red blood cell (RBC) surface. (fraunhofer.de)
  • In contrast to fluorescein and cyanine-based dyes, Atto 655 and Atto 647N have a more rigid molecular structure and are more photostable under prolonged light exposure (see Figure 1 ). (sigmaaldrich.com)
  • A class of dyes, BODIPY fluorophores, has been identified for automated DNA sequencing that has improved spectral characteristics compared with conventional fluorescein and rhodamine dyes. (sciencemag.org)
  • Of the green dyes, (visualized in the fluorescein channel) SYTO 11-16 and 20-24, we found #13 to be most effective. (wormbook.org)
  • The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. (osti.gov)
  • Two unique families of fluorophores are used, namely energy-transfer fluorescent beads and semiconductor nanocrystal (NC) quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. (osti.gov)
  • To make the technique truly noninvasive, scientists must further refine the dye so it fluoresces at a slightly longer wavelength, closer to the infrared region. (medgadget.com)
  • When light within a certain wavelength range hits a specific dye, the dye becomes "excited" and emits its own light, usually at a higher wavelength. (berkeley.edu)
  • In principle, the system can be adapted to any dye or nanoparticle able to oxidize DAB when excited by a specific wavelength of light. (frontiersin.org)
  • Because of the high fluorescence quantum yield ( ~ 0.68), APC is by far the brightest dye for the 650 nm wavelength region. (biotium.com)
  • Because of the high extinction coefficient ( ~ 1,960,000) and high fluorescence quantum yield ( ~ 0.82), R-PE is by far the brightest dye for the 565 nm wavelength region. (biotium.com)
  • The method consists of a fluorescent dye material, acting as a marker fluid, which is selectively responsive to ultraviolet light of a single wavelength. (trb.org)
  • Generally, a fluorescent dye will be excited by a coherent light source (a laser) at a particular wavelength and emit light at a lower energy and longer wavelength. (wikipedia.org)
  • JENA, Germany - Fluorescent dye-coated nanoparticles could be used to target and turn off disease-related genes. (photonics.com)
  • The biodegradable polymer nanoparticles were covered with near-infrared fluorescent polymethine dyes that made it possible to track the nanoparticles' movements in vivo using intravital microscopy or noninvasive multispectral optoacoustic tomography. (photonics.com)
  • Our fluorescent reagents and nanoparticles are optimized for use in the full range of optical in vivo imaging systems, as well as other fluorescence microscopy systems and many in vitro and cell-based systems. (perkinelmer.com)
  • The DyLight Dyes exhibit higher fluorescence intensity and photostability than Alexa™, CyDye™ and LI-COR™ Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). (fishersci.ca)
  • The high fluorescent potential and the exceptional photostability of lipophilic derivatives of perylene-3,4:9,10-bis(dicarboximides) are utilized for the fluorescence-labelling of liposomes. (uni-muenchen.de)
  • To overcome this issue of reduced resolution in STED imaging due to photodegradation, ITbM's team led by Principal Investigators Shigehiro Yamaguchi, a synthetic chemist and Tetsuya Higashiyama, a plant biologist have developed a new fluorescent dye, C-Naphox that has enhanced photostability relative to conventional dyes. (innovations-report.com)
  • The photostability of the newly synthesized dye was proven by irradiation experiments with a xenon lamp. (innovations-report.com)
  • Our comprehensive suite of fluorescent dyes, agents, and labeling kits enable unmatched imaging of a broad range of disease-related biomarkers and pathways in your research models. (perkinelmer.com)
  • The superior brightness and properties of our fluorescent dyes and labeling kits are ideal for labeling and imaging across a wide range of research areas. (perkinelmer.com)
  • The Janelia Fluor ® dyes are suitable to be used in conventional imaging techniques, such as confocal microscopy. (news-medical.net)
  • Not all fluorescent calcium indicators can be measured in a standard confocal microscope. (embl.de)
  • Until now, there hasn't been a general fluorescent dye that scientists could use to stain the material secreted by cells and view it under a confocal microscope. (acs.org)
  • Confocal fluorescence imaging of streptavidin-coated polystyrene microspheres (2 μm diameter) conjugated with biotinylated ODN3 duplex (left) or ODN3 duplex labeled with Cy3 (center) or Cy5 (right) FRET acceptor dyes. (nih.gov)
  • We described structural features of the Glia-neuromuscular synapses in a novel Inside-out tissue preparation of live fly larvae using fluorescent dyes with confocal microscopy. (jove.com)
  • In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. (mdpi.com)
  • AMRESCO's Fluorescent DNA Quantitation Kit quantifies double-stranded DNA in the low nanogram up to 5 microgram per ml range using fluorescent bisBenzimide H33258 (Hoechst) dye. (prweb.com)
  • The kit utilizes the fluorescent DNA intercalator, bisBenzimide H33258, otherwise known as Hoechst dye, and a simple method that may be easily scaled for a single cuvette or for high throughput in multi-well plates. (prweb.com)
  • In addition to a wide selection of gold standard labeling dyes such as DAPI, Hoechst, and JC-1, Enzo Life Sciences has translated its expertise in fluorescent probe chemistry and cellular analysis into our CELLestial® portfolio of unique probe-based assays and reagents to meet the emerging needs of the life sciences and drug discovery markets. (enzolifesciences.com)
  • Conventional fluorescent dyes for organelle detection & function including DAPI, Hoechst, JC-1, and more. (enzolifesciences.com)
  • Cells were counterstained with Hoechst® 33342 dye (blue, nuclei). (thermofisher.com)
  • While many commercially available fluorescent dyes have sufficed, most exhibit relatively low two-photon absorption (2PA) cross-section values in the tunability range of Ti:sapphire lasers commonly used in multiphoton microscopy imaging. (spie.org)
  • The shift in absorption and the induction of fluorescence effectively eliminate background effects from any unbound dye. (activemotif.com)
  • The slight red shift in absorption spectrum makes Fluorescent Dye 647-I Azide an optimal match to filters designed Cy5 dyes.Besides, their fluorescence is pH-independent from pH 3 to 11. (abnova.com)
  • Tocris has acquired a license for Janelia Fluor ® dyes and their photoactivatable derivatives from HHMI, Janelia Research Campus, making the first commercially available in 2017. (news-medical.net)
  • Near-Infrared Fluorescent Dye Maleimide Derivatives. (licor.com)
  • Indocyanine green (ICG) derivatives having nucleophilic substituents were synthesized as pH-responsive near-infrared dyes. (rsc.org)
  • Thiocyanate and triazinyl chloride derivatives of fluorescent dyes have been employed for the covalent labeling of components of the connective tissue of the rabbit cornea. (arvojournals.org)
  • In Part II the authors discuss reagents for labeling the endoplasmic reticulum and nuclear membranes (featuring the ER-Tracker™ dyes), Golgi apparatus (featuring dye-labeled ceramides), mitochondria (featuring the organic dye MitoTracker® Red CMXRos, Figure 2 ), and nucleoli (featuring the nucleic acid dye SYTO® RNASelect™ Green). (thermofisher.com)
  • Additionally, some amine-containing fluorescent reagents are also used to modify biomolecules, in particular, to label glycoproteins. (biomol.com)
  • Three major classes of amine-reactive fluorescent reagents are currently used to label biopolymers: succinimidyl esters (SE), isothiocyanates, and sulfonyl chlorides. (biomol.com)
  • Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. (osti.gov)
  • 4 Dyes such as Cy5, Cy5.5, or Alexa Fluor ® 647 may form cis-isomers and triplet states, which precludes their usage in demanding techniques including fluorescence correlation spectroscopy (FCS), single molecule detection (SMD), and as acceptors in fluorescence resonance energy transfer (FRET). (sigmaaldrich.com)
  • At the same time, the sensitivity of P-HOBDI-BF 2 -based assays remained high, and this fact together with acceptable fluorescence levels suggests that this dye can be considered as an efficient alternative for reporters traditionally used for fluorescence detection in the FAM channel. (springer.com)
  • ACDelco Coolant System Fluorescent Tracer Dye is a fluorescent leak-detection dye for traditional coolant systems. (acdelco.com)
  • Can I use fluorescent tracer dye for leak detection? (acdelco.com)
  • Yes, tracer dye is a great tool for leak detection. (acdelco.com)
  • The Fungi-Fluor ® Kit for Fungal Detection offers a quick fluorescent stain/counterstain procedure for various fungal organisms. (polysciences.com)
  • The sensitivity of DNA detection with AMRESCO's Fluorescent DNA Quantitation Kit ranges from the low nanogram range (10 ng/mL) up to 5 micrograms per mL. (prweb.com)
  • A new range of rhodamine-based fluorescent dyes, known as Janelia Fluor® Dyes has been recently developed by Luke Lavis' lab at the Janelia Research Campus, Howard Hughes Medical Institute (HHMI). (news-medical.net)
  • The advantage of Janelia Fluor ® dyes is that they are extremely bright and photostable. (news-medical.net)
  • Representative photoresistant fluorescent dyes such as Alexa Fluor® 488 and ATTO 488 are also known to encounter photodegradation in STED microscopy, making it difficult to conduct continuous live-imaging of biological systems while retaining high resolution. (innovations-report.com)
  • The Fungi Fluor ® Pneumocystis kit offers a fast, fluorescent staining procedure for Pneumocystis carinii in bronchial specimens. (polysciences.com)
  • PolySciTech division of Akina, Inc ( www.polyscitech.com ) provides a wide array of research products including Flamma Fluor dyes. (acs.org)
  • Novel dye selectively stains extracellular material red in fruit fly tissue. (acs.org)
  • Hydrolyzes to green-fluorescent calcein on contact with intracellular esterases in live cells. (abcam.com)
  • Fluorescent DNA nanotags featuring covalently attached intercalating dyes: synthesis, antibody conjugation, and intracellular imaging. (nih.gov)
  • The dye-modified DNA duplexes were then attached to a secondary antibody for intracellular fluorescence imaging of centrosomes in Drosophila embryos. (nih.gov)
  • pH-responsive dyes 1-C with closed-ring structures smoothly internalized and converted to emissive open-ring structures 1-O in response to relatively low pHs in acidic intracellular compartments of HeLa cells. (rsc.org)
  • Induction of apoptosis by intracellular potassium ion depletion : using the fluorescent dye PBFI in a 96-well plate method in cultured lung cancer cells. (diva-portal.org)
  • In order to determine intracellular K+ content, the potassium-binding fluorescent probe PBFI-AM was used in a 96-well plate assay. (diva-portal.org)
  • Certain fluorescent dyes can be used to characterize kinetic intracellular activity rather than fixing cells in formaldehyde and losing viable cells. (wikipedia.org)
  • This is the first report describing the possibility of using a green fluorescent protein chromophore synthetic analog, P-HOBDI-BF 2 , as a fluorescent dye for a linear hydrolysis probe used in qPCR. (springer.com)
  • We used a fluorescent lipophilic probe, CM-Dil, that avidly binds to the cell membrane. (nih.gov)
  • These unique dyes are used in a variety of NIR imaging applications including Western blotting , plate-based assays , protein arrays, tissue section imaging, probe development , and small animal imaging . (licor.com)
  • XenoLight RediJect 2-DeoxyGlucosone (DG) 750 is a fluorescent imaging probe for in vivo targeting of tumors that typically exhibit elevated glucose uptake rate in comparison to surrounding tissues. (perkinelmer.com)
  • A fluorescent probe is a fluorophore designed to localize within a specific region of a biological specimen or to respond to a specific stimulus. (biomol.com)
  • So which is best, dye or probe? (bitesizebio.com)
  • A fluorescent reporter is placed at the 5' end of the probe and a fluorescent quencher at the 3' end of the probe. (bitesizebio.com)
  • Please comment below with your preference of dye or probe. (bitesizebio.com)
  • A new photostable fluorescent dye for super resolution microscopy could serve as a powerful tool to visualize biological events and structural details in living cells at real-time for prolonged recording periods. (innovations-report.com)
  • Yet, the gradual degradation of fluorescent dyes when exposed to the high intensity light necessary for super resolution microscopy has been a major obstacle for long-term observations. (innovations-report.com)
  • Single and double BODIPY dye primers were characterized in commercially available DNA sequencers and showed uniform electrophoretic mobilities and high fluorescence intensities. (sciencemag.org)
  • Intravitreal injection of a fluorescent dye, Procion yellow, results in the complete and systematic staining of a cone population in the monkey retina. (nih.gov)
  • The high sensitivity of BODIPY dye primers requires at least 33 percent less reagent consumed per reaction than conventional dye primers, which should affect the costs of large genome-sequencing efforts. (sciencemag.org)
  • Under proper illumination, the dye provides a highly visible marker fluid, well-suited to conventional photography. (trb.org)
  • The results of the oil-contaminated aquifer study showed that conventional dye-tracing is possible in a heavily oil-contaminated aquifer despite the common thinking that organic dyes cannot be used in organic-rich environments. (syr.edu)
  • Green and Near-Infrared Fluorescent Dyes Make Cancer Cells Glow. (eurekalert.org)
  • As the spatial resolution of STED imaging correlates with an increase in STED light intensity, photodegradation of fluorescent dyes becomes a serious issue. (innovations-report.com)
  • Our results show that the decrease in electroluminescence intensity at high magnetic field, which survives only at low temperatures for pure Alq3-based devices, persists in dye-doped devices even at room temperature. (ebscohost.com)
  • Intercalating fluorescent insert into DNA during the process of amplification, so the intensity of fluorescence increases respectively, and you can make measurements in real-time. (bitesizebio.com)
  • This allowed the team to tune the spectral and chemical properties of the rhodamine dyes with unprecedented precision. (photonics.com)
  • ASHBURN, Va., Oct. 19, 2017 - To better illuminate the inner workings of cells, researchers have developed a way to adjust the properties of fluorescent dyes deliberately, resulting in an expanded palette of dyes that are bolder, brighter and more cell-permeable. (photonics.com)
  • Additionally, the water solubility of the DyLight Dyes allows a high dye-to-protein ratio without precipitation during conjugation. (fishersci.ca)
  • Figure 1: Color shift of Chromeo Py-Dyes caused by dye conjugation. (activemotif.com)
  • We here, using nanofluidic channels and fluorescence microscopy, investigate the heterogeneous staining on the single DNA molecule level and demonstrate that the dye distribution is continuous. (chalmers.se)
  • Improved overall sensitivity in biological analysis and imaging techniques can be obtained since Atto dyes have less interference from fluorophores with shorter lifetimes. (sigmaaldrich.com)
  • Chromeo Py-Dyes have been shown to work efficiently and with high sensitivity in different methods of protein electrophoresis. (activemotif.com)
  • Better accuracy and sensitivity can be achieved using AMRESCO's new Fluorescent DNA Quantitation Kit . (prweb.com)
  • The dye has low affinity for RNA, which improves specificity and sensitivity of the assay results when using impure samples. (prweb.com)
  • From simple organelle specific dyes for imaging cell structure and determining cell viability, to more complex dyes and reporter assays for monitoring cell signaling, death pathways, and toxicity, every product is developed and reliably manufactured to provide sensitivity, specificity, and convenience. (enzolifesciences.com)
  • Please always try a strand and sensitivity test prior to using any hair dye product. (beeunique.co.uk)
  • A correlation between LF and LF with dyes and mineral loss and receiver operating characteristics analysis are performed, as well as comparisons of sensitivity, specificity, and accuracy values. (spiedigitallibrary.org)
  • Fluorescent dsDNA dyes are frequently used to quantify dsDNA as they offer high sensitivity and specificity. (bmglabtech.com)
  • These dyes are also suitable for use in SRM techniques, including dSTORM. (news-medical.net)
  • The dye has minimal non-specific toxicity, provides a larger signal window, and is more suitable for longer term studies than other dyes. (selectscience.net)
  • This dye is not suitable for use on hair with more than 10% grey coverage. (bluebanana.com)
  • Bright fluorescent foci were observed at the centrosomes in both the donor (TO) and acceptor (Cy5) channels, because the energy transfer efficiency is moderate. (nih.gov)
  • Powder that turns snow or water into a bright fluorescent green signal. (alltackle.com)
  • There is also a large increase in quantum yield that changes the non- or only weakly fluorescent dye into a bright fluorescent conjugate . (activemotif.com)
  • Dye your water features to bright fluorescent red colour. (hydra-aqua.com)
  • bright fluorescent colors. (thefreedictionary.com)
  • However, current monitoring methods fall short, said the study's first author Evan W. Miller, a post-doctoral researcher in the lab of Roger Tsien, PhD, Howard Hughes Medical Institute investigator, UC San Diego professor of pharmacology, chemistry and biochemistry and 2008 Nobel Prize co-winner in chemistry for his work on green fluorescent protein. (ucsd.edu)
  • The labeled protein is ready to use immediately, and any possible background from unbound dye is eliminated. (activemotif.com)
  • Because of their unique features, Chromeo Py-Dyes (Chromeo P429, P503, P540 and P543) have been used successfully in a number of "no-wash" applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies. (activemotif.com)
  • Amine-reactive pyrylium dyes have been used as a pre-stain in protein electrophoresis. (activemotif.com)
  • fluorescent antibody test a test for the distribution of cells expressing a specific protein by binding antibody specific for the protein and detecting complexes by fluorescent labeling of the antibody. (thefreedictionary.com)
  • The mutant form GGBP/H152C of the D-glucose/D-galactose-binding protein with the solvatochromic dye BADAN linked to cysteine residue Cys 152 can be used as a potential base for a sensitive element of glucose biosensor system. (peerj.com)
  • The most common method for measuring protein thermal shifts is differential scanning fluorimetry (DSF) or thermofluor, which utilizes specialized fluorogenic dyes. (wikipedia.org)
  • When the protein unfolds, the exposed hydrophobic surfaces bind the dye, resulting in an increase in fluorescence by excluding water. (wikipedia.org)
  • With the extensive selection available, Atto dyes can replace commonly used fluorescent dyes. (sigmaaldrich.com)
  • The commonly used fluorescent dye YOYO-1 (YOYO) has, using bulk techniques, been demonstrated to stain DNA heterogeneously at substoichiometric concentrations. (chalmers.se)
  • The commonly used fluorescent dyes to detect mitochondria activity include Rhodamine 123 (R123), Mitotracker Green TM (17), Mitotracker Red CMXROs, Mitotracker Deep Red 633 (M-22426) (18), Mitotracker Orange TM (19), DiO[D.sub.6](3) and JC-1 (20). (thefreedictionary.com)
  • We have synthesized fluorescent DNA duplexes featuring multiple thiazole orange (TO) intercalating dyes covalently attached to the DNA via a triazole linkage. (nih.gov)
  • Smaller numbers of mycobacteria are easily identified with a fluorescent stain. (polysciences.com)
  • Fluorescent chromosome stain. (polysciences.com)
  • If you can identify which CD is presented on your cells of interest, then you can stain your sample with a fluorescent dye specific to it and using FACS, sorting out only those cells. (wikipedia.org)
  • The water-soluble dye is composed of a central perylene chromophore, a polyphenylene dendrimer scaffold, and a polymer shell bearing multiple amine groups. (acs.org)
  • The internal location of the microelectrode has been confirmed by electrophoretically microinjecting the water-soluble dye Lucifer yellow CH into giant mitochondria or mitoplasts. (sciencemag.org)
  • However, researchers can use a variety of fluorescent dyes to design multi-color panels to achieve successful, simultaneous sorting of multiple, precisely defined cell-types. (wikipedia.org)
  • The authors demonstrated the ability to use these dyes to detect cell death during drug screening in the article " Evaluation of Impermeant, DNA-Binding Dye Fluorescence as a Real-Time Readout of Eukaryotic Cell Toxicity in a High Throughput Screening Format . (eurekalert.org)
  • To detect the exact location of the coolant leak before it becomes a severe problem, use ACDelco Coolant System Fluorescent Tracer Dye. (acdelco.com)
  • So the dye in specific conditions, you can detect the infection," says Kanthasmy. (iowapublicradio.org)
  • This fluorescent tracer dye quickly pinpoints the exact location of coolant fluid leaks. (acdelco.com)
  • ACDelco Coolant System Fluorescent Tracer Dye may also be used to isolate power steering system leaks. (acdelco.com)
  • ACDelco Coolant System Fluorescent Tracer Dye can be used with DEX-COOL coolants, but it may change the color of the coolants. (acdelco.com)
  • For dye that will not change long life coolant color use ACDelco Coolant System Tracer Dye. (acdelco.com)
  • Dye, water cooled computer coolant for the decoration of, one fluid ounce (about 30cc). (dansdata.com)
  • Put a few drops of the dye into your coolant - because a little goes a long way - and install the blacklight in your case, and your see-through coolant tubing glows spooky green. (dansdata.com)
  • Multi-color organelle-specific dyes for co-localization studies and monitoring changes in cell structure. (enzolifesciences.com)
  • Among the reactive dyes, amine-reactive dyes are most often used to prepare various bioconjugates for immunochemistry, histochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor binding and other biological applications since amino groups are either abundant or easily introduced into biomolecules. (biomol.com)
  • Amine-reactive dyes are most often used to prepare bioconjugates for immunochemistry, fluorescence in situ hybridization (FISH), cell tracing, receptor labeling and fluorescent analog cytochemistry. (biomol.com)
  • Although conjugating dyes to biomolecules is usually easy, preparing the optimal conjugate may require extensive experimentation. (biomol.com)
  • Later, we devised indirect solutions such as calcium imaging or dyes that gave big but slow responses to voltage. (ucsd.edu)
  • Different brands and models of microscope offer different settings, so choose a fluorescent calcium indicator that suits yours (and also your experiment! (embl.de)
  • High-performance dyes for cell cycle analysis, calcium mobilization, cell lineage tracing, and more. (enzolifesciences.com)
  • CFSE is the most popular fluorescent dye for cell proliferation studies. (jimmunol.org)
  • Do you have NucView® substrates for other caspases or with different fluorescent dye colors? (biotium.com)
  • The new agents were molecularly altered to carry fluorescent dyes that glow under lights with specific wavelengths--either in the green (CLR1501) or near-infrared (CLR1502) range. (eurekalert.org)
  • Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. (osti.gov)
  • Many of Atto dyes (Atto 590 and above) can be excited using wavelengths greater than 600 nm. (sigmaaldrich.com)
  • Atto dyes exhibit longer fluorescence signal lifetimes (0.6-3.8 ns) than either carbocyanine dyes or most of the autofluorescence inherent in cells and biomolecules. (sigmaaldrich.com)
  • Fluorescent signals are stronger with the same molar amount of Atto 655 and Atto 680 since less dye is lost to inactivation . (sigmaaldrich.com)
  • Other Atto dyes also have low triplet formation. (sigmaaldrich.com)
  • ATTO-TEC® has developed the second generation of fluorescent dyes which are stable at room temperature for more than six months. (innovations-report.com)
  • With Atto 520, Atto 565 and Atto 590 we are pleased to offer three stable fluorescent dyes as amine-reactive succinimidyl esters which will be available from November 2001 on. (innovations-report.com)
  • Flow cytometry combined with fluorescent staining is a powerful tool for monitoring viable cells. (jimmunol.org)
  • Vio® Dyes represent a family of fluorochromes for flow cytometry and fluorescence microscopy developed by Miltenyi Biotec. (miltenyibiotec.com)
  • Fluorescent Activated Cell Sorting, or FACS, utilizes flow cytometry to provide a fast, objective and quantitative measurement of intra- and extracellular properties, not including morphology, for sorting a heterogeneous mixture of cells. (wikipedia.org)
  • Choosing a fluorescent reagent for imaging cellular structures and organelles in live or fixed cells can be daunting. (thermofisher.com)
  • Each fluorescent reagent comes with its own set of characteristics that may prove to be beneficial or detrimental for a particular assay or environmental condition. (thermofisher.com)
  • Researchers at the University of California, San Diego School of Medicine have created a new generation of fast-acting fluorescent dyes that optically highlight electrical activity in neuronal membranes. (ucsd.edu)
  • For their work, researchers at Howard Hughes Medical Institute (HHMI) focused on rhodamines, a family of fluorescent dyes that are especially bright and cell-permeable, but somewhat limited in their color range. (photonics.com)
  • The researchers grafted different chemicals into the rhodamines and analyzed the new dyes' properties. (photonics.com)
  • LI-COR IRDye ® infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. (licor.com)
  • Further research will be needed to assess issues related to dye administration, visualization, and timing, as well as the optimal technologies for practical use during surgery. (eurekalert.org)
  • One of the major problems of SLN mapping in colorectal cancer is the lack of an optimal dye and technique for identification of the nodes. (sages.org)
  • Current dyeing methods, including antibody-targeted staining, have been limited to visualizing one component or only certain kinds of tissues. (acs.org)
  • Easily label your antibody with phycobiliproteins APC, R-PE and PerCP, or tandem dyes APC-CF®750T and RPE-CF®647T, in about 4 hours. (biotium.com)
  • Typically, the dyes were dissolved in a hydrophilic organic solvent and then suddenly placed in an aqueous environment, where they spontaneously produce molecular assemblies. (coursehero.com)
  • In vivo tracking of bone marrow fibroblasts with fluorescent carbocyanine dye. (nih.gov)