Fluorescent antibody technique. Medical search

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

Process of growing viruses in live animals, plants, or cultured cells.

Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).

Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.

A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)

Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.

Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.

Substances that are recognized by the immune system and induce an immune reaction.

The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.

Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.

Immunoglobulins produced in response to VIRAL ANTIGENS.

The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.

Premature expulsion of the FETUS in animals.

A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)

The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.

Antibodies produced by a single clone of cells.

Immunoglobulins produced in a response to BACTERIAL ANTIGENS.

Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.

Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.

Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.

An acute inflammatory disease of the lower RESPIRATORY TRACT, caused by paramyxoviruses, occurring primarily in infants and young children; the viruses most commonly implicated are PARAINFLUENZA VIRUS TYPE 3; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; and METAPNEUMOVIRUS.

The processes triggered by interactions of ANTIBODIES with their ANTIGENS.

Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.

Substances elaborated by viruses that have antigenic activity.

Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.

A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.

A series of steps taken in order to conduct research.

The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.

Acute VIRAL CNS INFECTION affecting mammals, including humans. It is caused by RABIES VIRUS and usually spread by contamination with virus-laden saliva of bites inflicted by rabid animals. Important animal vectors include the dog, cat, bat, fox, raccoon, skunk, and wolf.

Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.

Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.

Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.

Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.

Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.

The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates.

A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.

Sites on an antigen that interact with specific antibodies.

The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).

Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)

Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.

Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)

Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.

Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.

Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.

Antibodies reactive with HIV ANTIGENS.

The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

Substances elaborated by bacteria that have antigenic activity.

Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)

A genus of the family RHABDOVIRIDAE that includes RABIES VIRUS and other rabies-like viruses.

Immunoglobulins induced by antigens specific for tumors other than the normally occurring HISTOCOMPATIBILITY ANTIGENS.

Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.

Diagnostic procedures involving immunoglobulin reactions.

A genus of protozoa found in reptiles, birds, and mammals, including humans. This heteroxenous parasite produces muscle cysts in intermediate hosts such as domestic herbivores (cattle, sheep, pigs) and rodents. Final hosts are predators such as dogs, cats, and man.

Acute inflammation of the intestine associated with infectious DIARRHEA of various etiologies, generally acquired by eating contaminated food containing TOXINS, BIOLOGICAL derived from BACTERIA or other microorganisms. Dysentery is characterized initially by watery FECES then by bloody mucoid stools. It is often associated with ABDOMINAL PAIN; FEVER; and DEHYDRATION.

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

An acute, sometimes fatal, pneumonia-like bacterial infection characterized by high fever, malaise, muscle aches, respiratory disorders and headache. It is named for an outbreak at the 1976 Philadelphia convention of the American Legion.

Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.

A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother.

Infection of the striated muscle of mammals by parasites of the genus SARCOCYSTIS. Disease symptoms such as vomiting, diarrhea, muscle weakness, and paralysis are produced by sarcocystin, a toxin produced by the organism.

A genus of flexible, spiral rods found in hydrogen sulfide-containing mud, sewage, and polluted water. None of the species properly referred to in this genus are pathogenic.

Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.

EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.

Diseases of domestic and wild horses of the species Equus caballus.

Diseases of domestic swine and of the wild boar of the genus Sus.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)

Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.

Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.

Immunoglobulins produced in a response to FUNGAL ANTIGENS.

Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

A general term indicating inflammation of the BRAIN and SPINAL CORD, often used to indicate an infectious process, but also applicable to a variety of autoimmune and toxic-metabolic conditions. There is significant overlap regarding the usage of this term and ENCEPHALITIS in the literature.

Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).

Established cell cultures that have the potential to propagate indefinitely.

Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.

Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.

Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.

A test to detect non-agglutinating ANTIBODIES against ERYTHROCYTES by use of anti-antibodies (the Coombs' reagent.) The direct test is applied to freshly drawn blood to detect antibody bound to circulating red cells. The indirect test is applied to serum to detect the presence of antibodies that can bind to red blood cells.

Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. (1/24343)

Studies of the actin-based motility of pathogens have provided important insights into the events occurring at the leading edge of motile cells [1] [2] [3]. To date, several actin-cytoskeleton-associated proteins have been implicated in the motility of Listeria or Shigella: vasodilator-stimulated phosphoprotein (VASP), vinculin and the actin-related protein complex of Arp2 and Arp3 [4] [5] [6] [7]. To further investigate the underlying mechanism of actin-tail assembly, we examined the localization of components of the actin cytoskeleton including Arp3, VASP, vinculin and zyxin during vaccinia, Listeria and Shigella infections. The most striking difference between the systems was that a phosphotyrosine signal was observed only at the site of vaccinia actin-tail assembly. Micro-injection experiments demonstrated that a phosphotyrosine protein plays an important role in vaccinia actin-tail formation. In addition, we observed a phosphotyrosine signal on clathrin-coated vesicles that have associated actin-tail-like structures and on endogenous vesicles in Xenopus egg extracts which are able to nucleate actin tails [8] [9]. Our observations indicate that a host phosphotyrosine protein is required for the nucleation of actin filaments by vaccinia and suggest that this phosphoprotein might be associated with cellular membranes that can nucleate actin. (+info)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (2/24343)

In COS cells, Ral GDP dissociation stimulator (RalGDS)-induced Ral activation was stimulated by RasG12V or a Rap1/Ras chimera in which the N-terminal region of Rap1 was ligated to the C-terminal region of Ras but not by Rap1G12V or a Ras/Rap1 chimera in which the N-terminal region of Ras was ligated to the C-terminal region of Rap1, although RalGDS interacted with these small GTP-binding proteins. When RasG12V, Ral and the Rap1/Ras chimera were individually expressed in NIH3T3 cells, they localized to the plasma membrane. Rap1Q63E and the Ras/Rap1 chimera were detected in the perinuclear region. When RalGDS was expressed alone, it was abundant in the cytoplasm. When coexpressed with RasG12V or the Rap1/Ras chimera, RalGDS was detected at the plasma membrane, whereas when coexpressed with Rap1Q63E or the Ras/Rap1 chimera, RalGDS was observed in the perinuclear region. RalGDS which was targeted to the plasma membrane by the addition of Ras farnesylation site (RalGDS-CAAX) activated Ral in the absence of RasG12V. Although RalGDS did not stimulate the dissociation of GDP from Ral in the absence of the GTP-bound form of Ras in a reconstitution assay using the liposomes, RalGDS-CAAX could stimulate it without Ras. RasG12V activated Raf-1 when they were coexpressed in Sf9 cells, whereas RasG12V did not affect the RalGDS activity. These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral, but that Rap1 does not activate Ral due to distinct subcellular localization. (+info)

Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast. (3/24343)

Cell division in a number of eukaryotes, including the fission yeast Schizosaccharomyces pombe, is achieved through a medially placed actomyosin-based contractile ring. Although several components of the actomyosin ring have been identified, the mechanisms regulating ring assembly are still not understood. Here, we show by biochemical and mutational studies that the S.pombe actomyosin ring component Cdc4p is a light chain associated with Myo2p, a myosin II heavy chain. Localization of Myo2p to the medial ring depended on Cdc4p function, whereas localization of Cdc4p at the division site was independent of Myo2p. Interestingly, the actin-binding and motor domains of Myo2p are not required for its accumulation at the division site although the motor activity of Myo2p is essential for assembly of a normal actomyosin ring. The initial assembly of Myo2p and Cdc4p at the division site requires a functional F-actin cytoskeleton. Once established, however, F-actin is not required for the maintenance of Cdc4p and Myo2p medial rings, suggesting that the attachment of Cdc4p and Myo2p to the division site involves proteins other than actin itself. (+info)

Arrestin function in G protein-coupled receptor endocytosis requires phosphoinositide binding. (4/24343)

Internalization of agonist-activated G protein-coupled receptors is mediated by non-visual arrestins, which also bind to clathrin and are therefore thought to act as adaptors in the endocytosis process. Phosphoinositides have been implicated in the regulation of intracellular receptor trafficking, and are known to bind to other coat components including AP-2, AP180 and COPI coatomer. Given these observations, we explored the possibility that phosphoinositides play a role in arrestin's function as an adaptor. High-affinity binding sites for phosphoinositides in beta-arrestin (arrestin2) and arrestin3 (beta-arrestin2) were identified, and dissimilar effects of phosphoinositide and inositol phosphate on arrestin interactions with clathrin and receptor were characterized. Alteration of three basic residues in arrestin3 abolished phosphoinositide binding with complete retention of clathrin and receptor binding. Unlike native protein, upon agonist activation, this mutant arrestin3 expressed in COS1 cells neither supported beta2-adrenergic receptor internalization nor did it concentrate in coated pits, although it was recruited to the plasma membrane. These findings indicate that phosphoinositide binding plays a critical regulatory role in delivery of the receptor-arrestin complex to coated pits, perhaps by providing, with activated receptor, a multi-point attachment of arrestin to the plasma membrane. (+info)

Coupling of the cell cycle and myogenesis through the cyclin D1-dependent interaction of MyoD with cdk4. (5/24343)

Proliferating myoblasts express the muscle determination factor, MyoD, throughout the cell cycle in the absence of differentiation. Here we show that a mitogen-sensitive mechanism, involving the direct interaction between MyoD and cdk4, restricts myoblast differentiation to cells that have entered into the G0 phase of the cell cycle under mitogen withdrawal. Interaction between MyoD and cdk4 disrupts MyoD DNA-binding, muscle-specific gene activation and myogenic conversion of 10T1/2 cells independently of cyclin D1 and the CAK activation of cdk4. Forced induction of cyclin D1 in myotubes results in the cytoplasmic to nuclear translocation of cdk4. The specific MyoD-cdk4 interaction in dividing myoblasts, coupled with the cyclin D1-dependent nuclear targeting of cdk4, suggests a mitogen-sensitive mechanism whereby cyclin D1 can regulate MyoD function and the onset of myogenesis by controlling the cellular location of cdk4 rather than the phosphorylation status of MyoD. (+info)

Retinoic acid, but not arsenic trioxide, degrades the PLZF/RARalpha fusion protein, without inducing terminal differentiation or apoptosis, in a RA-therapy resistant t(11;17)(q23;q21) APL patient. (6/24343)

Primary blasts of a t(11;17)(q23;q21) acute promyelocytic leukaemia (APL) patient were analysed with respect to retinoic acid (RA) and arsenic trioxide (As2O3) sensitivity as well as PLZF/RARalpha status. Although RA induced partial monocytic differentiation ex vivo, but not in vivo, As203 failed to induce apoptosis in culture, contrasting with t(15;17) APL and arguing against the clinical use of As203 in t(11;17)(q23;q21) APL. Prior to cell culture, PLZF/RARalpha was found to exactly co-localize with PML onto PML nuclear bodies. However upon cell culture, it quickly shifted towards microspeckles, its localization found in transfection experiments. Arsenic trioxide, known to induce aggregation of PML nuclear bodies, left the microspeckled PLZF/RARalpha localization completely unaffected. RA treatment led to PLZF/RARalpha degradation. However, this complete PLZF/RARalpha degradation was not accompanied by differentiation or apoptosis, which could suggest a contribution of the reciprocal RARalpha/PLZF fusion product in leukaemogenesis or the existence of irreversible changes induced by the chimera. (+info)

Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells. (7/24343)

The human granulocytic ehrlichiosis (HGE) agent resides and multiplies exclusively in cytoplasmic vacuoles of granulocytes. Double immunofluorescence labeling was used to characterize the nature of the HGE agent replicative inclusions and to compare them with inclusions containing the human monocytic ehrlichia, Ehrlichia chaffeensis, in HL-60 cells. Although both Ehrlichia spp. can coinfect HL-60 cells, they resided in separate inclusions. Inclusions of both Ehrlichia spp. were not labeled with either anti-lysosome-associated membrane protein 1 or anti-CD63. Accumulation of myeloperoxidase-positive granules were seen around HGE agent inclusions but not around E. chaffeensis inclusions. 3-(2, 4-Dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange were not localized to either inclusion type. Vacuolar-type H+-ATPase was not colocalized with HGE agent inclusions but was weakly colocalized with E. chaffeensis inclusions. E. chaffeensis inclusions were labeled with the transferrin receptor, early endosomal antigen 1, and rab5, but HGE agent inclusions were not. Some HGE agent and E. chaffeensis inclusions colocalized with major histocompatibility complex class I and II antigens. These two inclusions were not labeled for annexins I, II, IV, and VI; alpha-adaptin; clathrin heavy chain; or beta-coatomer protein. Vesicle-associated membrane protein 2 colocalized to both inclusions. The cation-independent mannose 6-phosphate receptor was not colocalized with either inclusion type. Endogenously synthesized sphingomyelin, from C6-NBD-ceramide, was not incorporated into either inclusion type. Brefeldin A did not affect the growth of either Ehrlichia sp. in HL-60 cells. These results suggest that the HGE agent resides in inclusions which are neither early nor late endosomes and does not fuse with lysosomes or Golgi-derived vesicles, while E. chaffeensis resides in an early endosomal compartment which accumulates the transferrin receptor. (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (8/24343)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space. (+info)

I: Demonstration of antibrain globulins by fluorescent antibody techniques. Arch Gen Psychiat 16: 1-9. (Schizophrenia, Blood ...

https://en.wikipedia.org/wiki/Taraxein

This can be done with direct fluorescent antibody (DFA) techniques. It can also be achieved through indirect immunofluorescence ... Another form of microscopy is fluorescent microscopy done by staining with auramine. Other staining techniques include acid- ... vaccine has produced an antibody response in a large group of cows and also antibody response in calves fed rCP15/60-immune ... Antibodies in the serum of humans and animals infected with Cryptosporidium parvum react with several antigens, one of which is ...

https://en.wikipedia.org/wiki/Cryptosporidiosis

Also, differential staining methods and fluorescent antibody techniques were introduced for in-situ observation of ... Various new techniques, ranging from the determination of growth and activity of microorganisms in the environments to their ... The new methods and techniques were proved to be successful for the analysis of microbial community in various fields, soil and ...

https://en.wikipedia.org/wiki/International_Society_for_Microbial_Ecology

... by fluorescent-antibody technique". Journal of Invertebrate Pathology. 11 (2): 251-259. doi:10.1016/0022-2011(68)90158-4. ...

https://en.wikipedia.org/wiki/Pseudomonas_melophthora

Later, Osborn and Weber pioneered fluorescent antibody staining of cellular substructures, a major technique called indirect ... then used fluorescently-tagged secondary antibodies (antibodies to the first set of antibodies) to light up the locations of ... and unreactive to actin antibodies. They developed new antibodies against proteins of the microtubules, intermediate filaments ... Many of their antibodies have been licensed to companies for commercial development. Klaus and Osborn used their method to ...

https://en.wikipedia.org/wiki/Mary_Osborn

Burgdorfer, W. Evaluation of the fluorescent antibody technique for the detection of Rocky Mountain spotted fever rickettsiae ... A technique for detection of rickettsiae in ticks, Amer. J. Trop. Med. 19:1010-1014, 1970. Burgdorfer, W., Barbour, A. G., ...

https://en.wikipedia.org/wiki/Willy_Burgdorfer

... fluorescent antibody and cell culture isolation techniques for detection of antigen". Journal of Fish Diseases. 7: 57-64. doi: ...

https://en.wikipedia.org/wiki/Spring_viraemia_of_carp

... he and two other researchers were the first to apply a recently developed technique, fluorescent antibody imaging, to the ... "Home". Sadun, E.H, Williams, J.S, Anderson, R.I. Fluorescent antibody technic for sero-diagnosis of schistosomiasis in humans. ... and is known for the first application of fluorescent antibody imaging in the diagnosis of parasitic diseases. Sadun was born ...

https://en.wikipedia.org/wiki/Elvio_Sadun

This distribution of this antibody can then be visualized by a technique such as fluorescent labeling. Immunohistochemistry has ... In this technique, DNA that encodes a reporter gene is randomly inserted into the genome. Depending on the gene promoters ... Techniques that require fixation of tissue can only generate a single temporal time point per individual organism. However, ... For example, the reporter gene green fluorescent protein can be visualized by stimulating it with blue light and then using a ...

https://en.wikipedia.org/wiki/Spatiotemporal_gene_expression

Adam Reich, Katarzyna Marcinow, and Rafal Bialynicki-Birula Direct+Fluorescent+Antibody+Technique at the US National Library of ... A direct fluorescent antibody (DFA or dFA), also known as "direct immunofluorescence", is an antibody that has been tagged in a ... Direct fluorescent antibody can also be used to detect parasitic infections, as was pioneered by Sadun, et al. (1960). ... where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached ...

https://en.wikipedia.org/wiki/Direct_fluorescent_antibody

To reduce the four-day waiting time needed for diagnosis, a method using the indirect fluorescent antibody technique (IFAT) was ...

https://en.wikipedia.org/wiki/Gaffkaemia

Immunohistochemistry is a technique that uses antibodies with fluorescent staining tags that target a specific antigen present ... He created an immunoflorescent technique for labelling the antibodies. This technique continues to be widely used in ... Albert Coons used for the first time a revolutionary technique that uses the principle of antibodies binding specifically to ... These examples use FISH (Fluorescent in situ hybridization). With this technique we can understand the physiological processes ...

https://en.wikipedia.org/wiki/Neuronal_lineage_marker

Benador Associates ^ "Detection of air-borne Pasteurella tularensis using the fluorescent antibody technique" by R. F. Jaeger, ...

https://en.wikipedia.org/wiki/Richard_O._Spertzel

... a historical and traditional province of Shewa in the Amhara region of Ethiopia Indirect fluorescent antibody technique, a ...

https://en.wikipedia.org/wiki/Ifat

... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... fluorescent antibody technique, direct MeSH E05.478.588.375.310 - fluorescent antibody technique, indirect MeSH E05.478.588.375 ... fluorescent antibody technique MeSH E05.478.588.375.050 - antibody-coated bacteria test, urinary MeSH E05.478.588.375.300 - ... 512.240.310 - fluorescent antibody technique, indirect MeSH E05.200.750.551.790 - periodic acid-schiff reaction MeSH E05.200. ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)

She is also well known for her work in the development of the fluorescent antibody technique-a diagnostic technique used to ... "Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum". The American Journal of Pathology. 34 (6): 1081-1097 ...

https://en.wikipedia.org/wiki/Cornelia_Mitchell_Downs

... fluorescent antibody technique, direct MeSH E01.450.495.225.230 - fluorescent antibody technique, indirect MeSH E01.450.495.225 ... fluorescent antibody technique MeSH E01.450.495.225.050 - antibody-coated bacteria test, urinary MeSH E01.450.495.225.225 - ... fluorescent treponemal antibody absorption test MeSH E01.450.495.735.850.800 - treponema immobilization test MeSH E01.450. ... enzyme multiplied immunoassay technique MeSH E01.450.495.410.380 - immunosorbent techniques MeSH E01.450.495.410.380.200 - ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E01)

... indirect fluorescent antibody testing, ELISA, PCR, and DNA probe technology techniques can be used to confirm diagnosis. The ...

https://en.wikipedia.org/wiki/Infectious_hematopoietic_necrosis_virus

... be cultured on specific growth mediums such as brain-heart infusion agar and techniques such as indirect fluorescent antibody ...

https://en.wikipedia.org/wiki/Edwardsiella_tarda

... an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen technique ... This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets ... recognizes the primary antibody and binds to it. Multiple secondary antibodies can bind a single primary antibody. This ... Since the number of fluorescent molecules that can be bound to the primary antibody is limited, direct immunofluorescence is ...

https://en.wikipedia.org/wiki/Immunofluorescence

... coli DNA extraction method plus PCR techniques. Newer technologies using fluorescent and antibody detection are also under ... Tzipori S, Sheoran A, Akiyoshi D, Donohue-Rolfe A, Trachtman H (October 2004). "Antibody therapy in the management of shiga ... such as the use of anti-induction strategies to prevent toxin production and the use of anti-Shiga toxin antibodies, have also ...

https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7

6: The fluorescent antibody test". In Kaplan MM, Koprowski H (eds.). Laboratory techniques in rabies. Monograph series. Vol. 23 ... The reference method for diagnosing rabies is the fluorescent antibody test (FAT), an immunohistochemistry procedure, which is ... to bind to and allow the visualisation of rabies antigen using fluorescent microscopy techniques. Microscopic analysis of ... Bat rabies antibodies (but not the virus) have been found in bats. On Svalbard, animals can cross the arctic ice from Greenland ...

https://en.wikipedia.org/wiki/Rabies

These cells are STED fluorescent dyes bound to antibodies through amide bonds. The first use of this technique coupled MR-121SE ... a red dye, with a secondary anti-mouse antibody. Since that first application, this technique has been applied to a much wider ... However, only fluorescent proteins provide the ability to visualize any organelle or protein in a living cell. This method was ... Using two fluorescent dyes and beam pairs, colocalized imaging of synaptic and mitochondrial protein clusters is possible with ...

https://en.wikipedia.org/wiki/STED_microscopy

Antiechinococcus antibodies can be detected with serodiagnostic tests - indirect fluorescent antibody, complement fixation, ... Cysts are detected with ultrasound, X-ray computed tomography, or other imaging techniques. ...

https://en.wikipedia.org/wiki/Echinococcus

After expansion, the tissue is far less dense and often allows for better reception of fluorescent antibodies. Markoff J (2015- ... However, this technique has since been adopted into many different fields of research and continues to grow and be applied in ... "Expansion microscopy with conventional antibodies and fluorescent proteins". Nature Methods. 13 (6): 485-8. doi:10.1038/nmeth. ... Expansion microscopy with conventional antibodies and fluorescent proteins. Nature Methods, 2016, 13, 485-488. "Microscopy ...

https://en.wikipedia.org/wiki/Expansion_microscopy

They are designed for labeling biomolecules, cells, tissues or beads in advanced fluorescent detection techniques. FluoProbes ... while FluoProbes labeled antibodies and cellular probes (i.e. Phalloidin) suit direct use in immunoassays or cell assays. ... Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes. "FluoProbes Dyes" (PDF). Interchim. 2010. ... The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. ...

https://en.wikipedia.org/wiki/FluoProbes

... such as fluorescent staining, multi-angle light scatter and monoclonal antibody tagging. Most analyzers directly measure the ... In light scattering techniques, light from a laser or a tungsten-halogen lamp is directed at the stream of cells to collect ... These techniques work on the same principle of measuring the interruption in current as cells pass through an aperture, but ... Often, these antibodies are only active at room temperature (in which case they are called cold agglutinins), and the ...

https://en.wikipedia.org/wiki/Complete_blood_count

... or by fluorescent antibodies. The technology may be used to determine whether a potential drug is disease modifying. For ... in the amounts of proteins synthesized by cells are measured using a variety of techniques such as the green fluorescent ... The wide use of the green fluorescent protein, a natural fluorescent protein molecule from jellyfish, then accelerated the ... In addition to fluorescent labeling, various label free assays have been used in high content screening. High-content screening ...

https://en.wikipedia.org/wiki/High-content_screening

... fluorescent protein based methods, and mass-spectroscopy based methods. The antibody based methods use designed antibodies to ... Secondary ion mass spectrometry (SIMS) is a technique similar to DESI, but while DESI is an ambient ionization technique, SIMS ... Imaging: Antibodies can be bound to fluorescent molecules such as quantum dots or tagged with organic fluorophores for ... Antibody-DNA quantification: another antibody-based method converts protein levels to DNA levels. The conversion to DNA makes ...

https://en.wikipedia.org/wiki/Single-cell_analysis

In a separate study, antibody-antigen binding was studied without the need for a fluorescent label to be added to the antibody ... Other microscopy techniques are commonly used in tandem with NanoSIMS that allow for multiple types of information to be ... Immunogold labeling uses antibodies to target specific proteins, and subsequently labels the antibodies with gold nano ... This technique can be used to study nutrient exchange. The mouse gut microbiome was investigated to determine which microbes ...

https://en.wikipedia.org/wiki/Nanoscale_secondary_ion_mass_spectrometry

... given the wide selection of fluorescent labels available today. For example, a technique known as calcium imaging measures the ... "Novel Bead-Based Epitope Assay is a sensitive and reliable tool for profiling epitope-specific antibody repertoire in food ... non-fluorescent molecule: binding results in a slower rotation speed of the fluorescent molecule, and in an increase in the ... A high level of polarization indicates that fluorescent is attached to a larger molecular complex. As a result, one of the ...

https://en.wikipedia.org/wiki/Plate_reader

... (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only ... or imaged simultaneously with a fluorescent antibody assay. The technology has potential applications in cancer diagnosis, ... FISH is a very general technique. The differences between the various FISH techniques are usually due to variations in the ... A variety of other techniques uses mixtures of differently colored probes. A range of colors in mixtures of fluorescent dyes ...

https://en.wikipedia.org/wiki/Fluorescence_in_situ_hybridization

Microscopy can be used for identification when bacteria are stained with fluorescent antibody stain. Histophilus species can be ... joint or pleural fluids for bacterial DNA via PCR or bacterial culture techniques. Although it is difficult to diagnose H. ... there are many cross reactive antibodies, there are conflicting effects due to herd vaccination and there is asymptomatic ... herds using a microagglutination test but this also proves difficult because clinically healthy herds might have high antibody ...

https://en.wikipedia.org/wiki/Histophilus_somni

... and fluorescent antibodies and more recently chemiluminescence. In microbiology, serologic tests are used to determine if a ... These surveys are potentially the most direct and informative technique available to infer the dynamics of a population's ... In practice, the term usually refers to the diagnostic identification of antibodies in the serum. Such antibodies are typically ... The amount of antibody in each specimen (antibody titer) is compared, and a significantly higher amount of IgG in the ...

https://en.wikipedia.org/wiki/Serology

After amplifying and then concentrating any PrPSc, the samples are labelled with a fluorescent dye using an antibody for ... The technique allowed detection of PrPSc after many fewer cycles of conversion than others have achieved, substantially ... method combines amplification with a novel technology called surround optical fiber immunoassay and some specific antibodies ...

https://en.wikipedia.org/wiki/Bovine_spongiform_encephalopathy

... technique. Like the locus-specific DNA methylation assay, the technique identified methylated DNA via its digestion methylation ... the fluorescent tag is cleaved off and the detector detects the fluorescent signal of the nucleotide incorporation. As the ... Modification-specific antibodies in turn, are used to immunoprecipitate the DNA-histone complexes. Following ... This technique has been used to such an extent that nucleosome-free regions have been labelled as DHSs, DNase I hypersensitive ...

https://en.wikipedia.org/wiki/Epigenomics

Detection is performed using either a primary or a secondary labeled antibody by chemiluminescent, fluorescent or colorimetric ... if fluorescent techniques are used. Two programs available online (P-SCAN and ProteinScan) can then be used to convert the ... Strips with single band indicate specific antibodies that are suitable for RPMA use. Antibody performance should be also ... In addition, finding the appropriate antibody could require extensive screening of many antibodies by western blotting prior to ...

https://en.wikipedia.org/wiki/Reverse_phase_protein_lysate_microarray

A large range of analytes of biological interest such as proteins, DNA, cancer cells, glucose and antibodies can be detected ... "A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves". Sensors. 13 (2): 2011- ... "Simple and Precise Preparation of a Porous Gel for a Colorimetric Glucose Sensor by a Templating Technique". Angewandte Chemie ... Emiliyanov, Grigoriy; Høiby, Poul; Pedersen, Lars; Bang, Ole (2013-03-08). "Selective Serial Multi-Antibody Biosensing with ...

https://en.wikipedia.org/wiki/Photonic_crystal_sensor

CS1 errors: missing periodical, Biochemistry, Biotechnology, Laboratory techniques, Molecular biology techniques, Protein ... FSL have been used to create human red cell kodecytes that have been used to detect and identify blood group allo-antibodies as ... doi:10.1111/j.1751-2824.2011.01505.x. Lan, C-C; Blake, D; Henry, S; Love, D R (2012). "Fluorescent Function-Spacer-Lipid ... Nadarajan, V.S.; Laing, A. A.; Saad, S. M.; Usin, M (2011). "Prevalence and specificity of red-blood-cell antibodies in a ...

https://en.wikipedia.org/wiki/Function-spacer-lipid_Kode_construct

It is widely used as a fluorescent tracer for many applications. The color of its aqueous solutions is green by reflection and ... Käss, W. Tracing Technique in Geohydrology. Rotterdam: Balkema. The Story Behind Dyeing the River Green. Greenchicagoriver.com ... Fluorescein-labelled probes can be imaged using FISH, or targeted by antibodies using immunohistochemistry. The latter is a ... The fluorescein that has been taken up into the plant can be visualized under a fluorescent microscope. Chemical derivatives of ...

https://en.wikipedia.org/wiki/Fluorescein

1996). "Antibodies to Orientia tsutsugamushi in Thai soldiers". Am J Trop Med Hyg. 55 (5): 556-9. doi:10.4269/ajtmh.1996.55.556 ... The gold standard is indirect immunofluorescence, but the main limitation of this method is the availability of fluorescent ... by the indirect immunoperoxidase technique". J Clin Microbiol. 15 (6): 1128-l. doi:10.1128/JCM.15.6.1128-1132.1982. PMC 272264 ... Serological methods are most reliable when a four-fold rise in antibody titre is found. If the patient is from a nonendemic ...

https://en.wikipedia.org/wiki/Scrub_typhus

Direct analyzation methods based on enzymatic/fluorescent detection (e.g. HRP, fluorescent dye) can be used for on-bead ... using an antibody from a complex mixture. The extract of disrupted tissue or cells is mixed with an antibody against the ... Using this technique combination, one can determine if a specific antigen is synthesized by a tissue or by a cell. Multiwell ... Such techniques are also available to create receptor antagonists in order to prevent further cascades. This provides ...

https://en.wikipedia.org/wiki/Ligand_binding_assay

Using X-ray crystallography and other techniques, Lippard and his coworkers have examined the mechanisms involved in binding ... Pentarins are nanoparticle drugs, similar to antibody-drug conjugates but smaller, that have been described as "mini-smart ... Working at the interface of inorganic chemistry and neuroscience, he has devised fluorescent imaging agents for studying mobile ... An overview of analytical techniques, approaches, and probes". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. ...

https://en.wikipedia.org/wiki/Stephen_J._Lippard

A natural lipid bilayer is not fluorescent, so at least one fluorescent dye needs to be attached to some of the molecules in ... Most techniques require sophisticated microscopy and very sensitive measurement equipment. In contrast to Ka, which is a ... More recently[when?] work has been undertaken to graft antibodies or other molecular markers onto the liposome surface in the ... A sample is excited with one wavelength of light and observed in another, so that only fluorescent molecules with a matching ...

https://en.wikipedia.org/wiki/Lipid_bilayer

Most fluorescent DNA dyes (one of exceptions is Hoechst 33342) are not plasma membrane permeant, that is, unable to pass ... Biological techniques and tools, Cell biology, Cell cycle, Flow cytometry). ... deoxyuridine antibodies". BioTechniques. 44 (7): 927-9. doi:10.2144/000112812. PMID 18533904. Vindeløv LL, Christensen IJ, ... Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as ...

https://en.wikipedia.org/wiki/Cell_cycle_analysis

The main nonculture tests include fluorescent monoclonal antibody test, enzyme immunoassay, DNA probes, rapid Chlamydia tests ... A major re-description of the Chlamydiales order in 1999, using the then new techniques of DNA analysis, split three of the ... the second detects a colored product converted by an enzyme linked to an antibody. The rapid Chlamydia tests use antibodies ... In 2009 the validity of Chlamydophila was challenged by newer DNA analysis techniques, leading to a proposal to "reunite the ...

https://en.wikipedia.org/wiki/Chlamydia_(genus)

It allows for antibody identification of cells expressing the target protein. Reporter genes: Some vectors may contain a ... Freshney IR (2005-07-29). Culture of Animal Cells: A manual of basic technique. Hoboken, New Jersey: John Wiley & Sons, Inc. ... Other commonly used reporters include green fluorescent protein and luciferase. Targeting sequence: Expression vectors may ...

https://en.wikipedia.org/wiki/Vector_(molecular_biology)

In sandwich assays an enzyme-labelled antibody is used; in competitive assays an enzyme-labelled antigen is used. On antibody- ... Although this technique is very powerful in that many sensors can be created simultaneously, it is currently only feasible for ... Secondly in 1986 Hood and co-workers devised a method to label DNA molecules with fluorescent tags instead of radiolabels, thus ... Each bead was uniquely encoded with a fluorescent signature. However, this encoding scheme is limited in the number of unique ...

https://en.wikipedia.org/wiki/Biochip

"Actin antibody: the specific visualization of actin filaments in non-muscle cells". The fluorescent signal could be easily ... This technique has also become a routine part of lab practice all around the world. He was a coauthor on a third fundamentally ... other cellular structures with specific antibodies and then tag these antibodies with a second fluorescently labelled antibody ... In summary, he contributed to the development of three of the most important and routinely used lab techniques. Among his other ...

https://en.wikipedia.org/wiki/Klaus_Weber

These proteins can be stained with fluorescent dye labeled antibodies and detected using flow cytometry. The limit of detection ... Some new techniques use Next-Generation Sequencing to detect MRD. These are based on detecting a leukemic specific RNA sequence ... treatment with monoclonal antibodies which target cancer cells cancer vaccines It is important that doctors interpreting tests ... Generally this is achieved through the use of the polymerase chain reaction, a highly sensitive technique that underpins much ...

https://en.wikipedia.org/wiki/Minimal_residual_disease

... antibodies labeled with radioactive isotopes for quantifying the levels of IgE antibody in the blood. Other, newer methods use ... Revue Scientifique et Technique. 19 (1): 240-58. doi:10.20506/rst.19.1.1218. PMID 11189719. Carvalho EM, Bastos LS, Araújo MI ( ... and fluorescent enzyme immunoassay (FEIA). Challenge testing: Challenge testing is when tiny amounts of a suspected allergen ... A rule of thumb is that the higher the IgE antibody value, the greater the likelihood of symptoms. Allergens found at low ...

https://en.wikipedia.org/wiki/Allergy

Genetics techniques, Laboratory techniques, Biological techniques and tools). ... antibody-phosphatase binding to RNA-probe (some hours) staining of antibody (e.g., with alkaline phosphatase) The protocol ... Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (RNA in ... The key techniques currently in use include in situ hybridization to mRNA with oligonucleotide and RNA probes (both radio- ...

https://en.wikipedia.org/wiki/In_situ_hybridization

... by binding phycobiliproteins to antibodies in a technique known as immunofluorescence. There are four types of phycobilins: ... Articles containing Ancient Greek (to 1453)-language text, Photosynthetic pigments, Tetrapyrroles, Fluorescent dyes). ...

https://en.wikipedia.org/wiki/Phycobilin

Hence, immunofluorescence refers to the detection of a fluorescent antibody (immuno) and immunoperoxidase refers to the ... This technique makes many copies of the virus genome using virus-specific probes. Variations of PCR such as nested reverse ... Both types of antibodies are measured when tests for immunity are carried out. Antibody testing has become widely available. It ... To do this, antibodies that are specific to different types of viruses are mixed with the tissue sample. After the tissue is ...

https://en.wikipedia.org/wiki/Laboratory_diagnosis_of_viral_infections

Early phage display techniques in the 1980s allowed targeting of mutations and selection to a single protein. This enabled ... Hawkins RE, Russell SJ, Winter G (August 1992). "Selection of phage antibodies by binding affinity. Mimicking affinity ... Nguyen AW, Daugherty PS (March 2005). "Evolutionary optimization of fluorescent proteins for intracellular FRET". Nature ... Methods to evolve enzymes were developed in the 1990s and brought the technique to a wider scientific audience. The field ...

https://en.wikipedia.org/wiki/Directed_evolution

Proliferating Daoy cells were placed on a glass coverslip, fixed and stained with fluorescent antibodies for survivin and alpha ... The idea and general principle behind his technique is described below. Mice were immunized with the oral vaccination and then ... To see which subcellular compartments contained the survivin splice variants complexes, fluorescent antibody markers for ... B. Humoral Antibody Response Taking blood samples from cancer patients, scientists have found antibodies that are specific for ...

https://en.wikipedia.org/wiki/Survivin

... through the presence of naturally occurring antibodies. Significant amounts of anti Z-DNA antibodies were found in SLE patients ... This was performed by measuring the intensity values between the donor and acceptor fluorescent dyes, also known as ... Furthermore, using micrococcal nuclease-crosslinking technique, Z-DNA is unlikely to form nucleosomes, which are often located ... Lafer, E M; Valle, R P; Möller, A; Nordheim, A; Schur, P H; Rich, A; Stollar, B D (1983-02-01). "Z-DNA-specific antibodies in ...

https://en.wikipedia.org/wiki/Z-DNA

Laboratory techniques, DNA profiling techniques, Amplifiers, Roche, Biotechnology, Molecular biology techniques, American ... However, antibodies don't appear until many weeks after infection, maternal antibodies mask the infection of a newborn, and ... The first method consists of using fluorescent dyes that are retained nonspecifically in between the double strands. The second ... Through this combined technique, mRNA is converted to cDNA, which is further quantified using qPCR. This technique lowers the ...

https://en.wikipedia.org/wiki/Polymerase_chain_reaction

The fluorescent antibody technique as a measure of antibody to malaria parasites / by A. Voller and R. S. Bray ... Antibody levels detected by the fluorescent antibody technique in mice infected with Plasmodium vinckei / by F. E. G. Cox, ... The Fluorescent antibody technique as applied to malaria World Health Organization (‎World Health OrganizationWorld Health ... Fluorescent antibody technique applied to the study of serological cross-reactions of exo-erythrocytic schizonts of avian, ...

https://extranet.who.int/iris/restricted/browse?authority=Fluorescent+Antibody+Technique&type=mesh

Bacteriological Techniques Diarrhea, Infantile Escherichia Coli False Positive Reactions Feces Fluorescent Antibody Technique ... Title : Fluorescent antibody techniques for Salmonella and other enteric pathogens. Personal Author(s) : Cherry, W. B.;Thomason ... Fluroescent antibody technique for identification of presumptively positive gonococcal cultures. Cite CITE. Title : Fluroescent ... antibody technique for identification of presumptively positive gonococcal cultures. Personal Author(s) : Peacock, William L.; ...

https://stacks.cdc.gov/view/cdc/74997

The Fluorescent antibody technique as applied to malaria World Health Organization (‎WHO/Mal/69.703, 1969)‎ ...

https://apps.who.int/iris/discover?rpp=10&etal=0&group_by=none&page=4&filtertype_0=author&filtertype_1=dateIssued&filtertype_2=dateIssued&filter_relational_operator_1=equals&filter_relational_operator_0=equals&filter_2=%5B1960+TO+1999%5D&filter_1=%5B1960+TO+1969%5D&filter_relational_operator_2=equals&filter_0=World+Health+Organization

Results of search for su:{Fluorescent antibody technique.} Refine your search. *. Availability. * Limit to currently ... A fluorescent-antibody technique for the detection of enterotoxin-producing cells of Clostridium perfringens type A / Manuel J ... Immunofluorescence techniques in diagnostic microbiology / edited by Joan M. B. Edwards, C. E. D. Taylor, A. H. Tomlinson. by ...

https://kohahq.searo.who.int/cgi-bin/koha/opac-search.pl?q=su:%7BFluorescent%20antibody%20technique.%7D

Koshi G, Chacko J. Rapid diagnosis of bacterial meningitis by the fluorescent-antibody (FA) technique. Indian Journal of ...

https://imsear.searo.who.int/handle/123456789/16702

Fluorescent Antibody Technique * Glycogen Synthase Kinase 3 / genetics * Glycogen Synthase Kinase 3 / metabolism* ...

https://pubmed.ncbi.nlm.nih.gov/17543867/?dopt=Abstract

... indirect fluorescent antibody technique; ND, not done; CSF, cerebrospinal fluid.. †The ELISA is positive if ratio ,1.1 for IgM ...

https://wwwnc.cdc.gov/eid/article/19/4/12-1594-t1

antigen by the direct fluorescent antibody technique is not suitable for environmental samples. ... Collection and culture techniques are outlined (Box C.2). Swabs can be streaked directly onto buffered charcoal yeast extract ... Water Sampling Strategies and Culture Techniques for Detecting Legionellae *Box C.1. Potential sampling sites for Legionella ... 3. Water Sampling Strategies and Culture Techniques for Detecting Legionellae. Legionella spp. are ubiquitous and can be ...

https://www.cdc.gov/infectioncontrol/guidelines/environmental/appendix/water.html

Fluorescent Antibody Technique E1.450.495.225 E1.450.495.435.375. E5.478.588.375 E5.478.594.430.375. Fluorescent Antibody ... Fluorescent Antibody Technique, Indirect. E1.450.495.225.230 E1.450.495.435.375.310. E5.478.588.375.310 E5.478.594.430.375.310 ... Fluorescent Treponemal Antibody-Absorption Test. E5.478.594.760.850.200. Fluoroimmunoassay E1.450.495.225.240 E1.450.495.435. ... Enzyme Multiplied Immunoassay Technique. E5.478.567.350.180 E1.450.495.435.400.180. E5.478.588.400.180 E5.478.594.410.350.180. ...

https://decs.bvs.br/P/mnchg2008.htm

Fluorescent antibody technique. Porphyria cutanea tarda/complications. Porphyria cutanea tarda/pathology. Porphyria cutanea ...

https://www.teses.usp.br/teses/disponiveis/5/5133/tde-11102006-105501/pt-br.php

Categories: Fluorescent Antibody Technique Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, ...

https://phil.cdc.gov/AdvancedSearchResults.aspx?Search=Fluorescent+Antibody+Technique&parentid=13864&catid=10829

Infected contacts may be identified rapidly by the fluorescent antibody (FA) technique; however, culture techniques identify ... Sensitive screening techniques exist, for example, fluorescent antibody to membrane antigen (FAMA), immune adherence ... Techniques are not yet available to detect specific antigens and antibodies or to determine the period of infectivity after ... The Mantoux technique using 5 TU PPD should be used. Category II * The 2-step test should be used to minimize the likelihood of ...

https://wonder.cdc.gov/wonder/prevguid/p0000446/p0000446.asp

... fluorescent antibody technique; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics; viruses; Show ...

https://pubag.nal.usda.gov/?q=%22Influenza+A+virus%22&search_field=subject

Detection and Identification of Streptococcus Iniae Using a Monoclonal Antibody-Based Indirect Fluorescent Antibody Technique ( ...

https://www.ars.usda.gov/research/publications/publications-at-this-location/?modecode=60-40-10-10&locpubs=yes&filterYear=2005

... and fluorescent-antibody techniques (4). Paramushir was isolated from Ixodes uriae and I. signatus in the eastern USSR (3,4). ...

https://wwwn.cdc.gov/ArboCAT/VirusDetails.aspx?ID=38&SID=14

Sputum examination may be supplemented by using a Legionella -specific fluorescent antibody. However, this technique has a high ... Antigen-antibody testing has little clinical effect in an emergency department setting, although it may help in recalcitrant or ... A Legionella serum antibody titer of 1:128 or more is suggestive of the diagnosis. Pneumococcal antigen tests for serum, urine ... Chacko R, Rajan A, Lionel P, Thilagavathi M, Yadav B, Premkumar J. Oral decontamination techniques and ventilator-associated ...

https://www.medscape.com/answers/300157-19152/what-are-the-radiographic-findings-of-k-pneumoniae-lobar-pneumonia

Fluorescent Antibody Technique Medicine & Life Sciences 4% View full fingerprint Cite this. * APA ...

https://pure.qub.ac.uk/en/publications/targeting-treatment-resistant-breast-cancer-stem-cells-with-fkbpl

Gerencser, M. A. & Slack, J. M.(1976). Serological identification of Actinomyces using fluorescent antibody techniques. J Dent ... In Nucleic Acid Techniques in Bacterial Systematics, pp. 115-175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley ...

https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000950-0

Diagnosis of rabies in tested animals was by direct fluorescent antibody technique (DFAT). A positive reaction was demonstrated ... Continued research towards the development of novel diagnostic techniques and newer vaccines and techniques are also desired. ... by visualization of bright apple-green fluorescent particles ranging in size and morphology from dust-like particles to ...

http://www.emro.who.int/ar/emhj-volume-21-2015/volume-21-issue-8/epidemiology-of-rabies-in-oman-a-retrospective-study-19912013.html

Non-fluorescent TiO2 NPs were detected using simple microscopic techniques with the scFv antibody isolated against them. The ... The novel antibodies isolated when used in conjunction with other existing techniques for NP detection will comprise a powerful ... Confocal and fluorescence microscopy are also common techniques used to detect fluorescent NPs, however their detection ability ... antibodies each displayed monovalently on the gene III coat protein of a M13 filamentous phage. The scFv antibodies are ...

http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=+%27ultrafine%2A%27+OR+%28%27nano%2A%27+AND+%27particle%2A%27%29+OR+%27nanotech%2A%27+OR+%27nanomaterial%2A%27+OR+%27nanoparticle%2A%27+OR+%27nanotube%2A%27+&f1=%2A&Startyear=&terms=3&Adv=1&ct=&B1=Search&Limit=25000&Sort=DP+DESC&whichdate=DP&D1=10&EndYear=&PageNo=76&RecNo=754&View=f&

Fluorescent Antibody Technique 46% 89 Scopus citations * Do cannabinoids reduce multiple sclerosis-related spasticity?. Thaera ... Are corticosteroids efficacious for preventing or treating neutralizing antibodies in multiple sclerosis patients treated with ...

https://mayoclinic.pure.elsevier.com/en/persons/dean-marko-wingerchuk/publications/?page=2

The virus can also be identified using fluorescent antibody, complement fixation, and ELISA techniques. There is no treatment ... Suspected cases can also be verified using an immunofluorescent antibody technique on frozen tissue. Transmission is horizontal ... Disease and pathogen confirmation are based on clinical signs, species, histopathologic findings, immunofluorescent antibody ...

https://www.merckvetmanual.com/exotic-and-laboratory-animals/aquaculture/viral-diseases-in-aquaculture

... fluorescent antibody technique, GTP-binding proteins, gene knockout techniques, humans, lysosomes, membrane fusion, mice, ...

https://research.aston.ac.uk/en/publications/transglutaminase-2-is-involved-in-autophagosome-maturation

Indirect Fluorescent Antibody Technique Medicine & Life Sciences 16% * Chromatin Medicine & Life Sciences 13% ...

https://jhu.pure.elsevier.com/en/publications/immunofluorescent-study-of-histone-h5-in-chick-erythroid-cells-fr-4

In this technique, antibodies against distemper virus are tagged with fluorescent markers. The antibodies bind to virus if it ... DISTEMPER ANTIBODY LEVELS - Distemper titers (another word of "antibody level") of either the "IgM" type (produced in early ... Distemper antibodies in cerebrospinal fluid is highly indicative of distemper infection as vaccine-induced antibodies do not ... CEREBROSPINAL FLUID ANTIBODY LEVELS - In neurologic distemper cases, cerebrospinal fluid is often tapped and distemper antibody ...

https://marvistavet.com/canine-distemper.pml

... fluorescent antibody technique, human, leukocytoclastic vasculitis, male, pathology, photosensitivity disorder, porphyria, ... Fluorescent Antibody Technique, Humans, Iron, Male, Photosensitivity Disorders, Protoporphyria, Erythropoietic, Vasculitis, ...

https://research-repository.uwa.edu.au/en/publications/leukocytoclastic-vasculitis-masking-chronic-vascular-changes-in-p

This specimen was prepared using the fluorescent antibody (FA) staining technique, which yielded this positive result.. ...

https://phil.cdc.gov/Details.aspx?pid=21315

Fluorescent Antibody Technique. Humans. Peptide Fragments. RNA-Binding Proteins. Transcription, Genetic. Tumor Cells, Cultured ... fluorescent antibody technique. genetic transcription. genetics. metabolism. pathology. tumor cell culture. Western blotting. ...

https://scholarbank.nus.edu.sg/handle/10635/180864

Fluorescent Dyes (1) Fluorescent Antibody Technique -... (1) Crystallization - Methods Breast Neoplasms - Pathology (1) ... Diverse Techniques Diagnostic Imaging - Methods (1) more .... Diagnostic Imaging - Instrumenta... (1) Fibroblasts - Metabolism ... topic:Diverse Techniques. topic:Crystallization - Methods. topic:Fundamental And Applied Biological Sciences. Psychology ...

https://beluga.sub.uni-hamburg.de/vufind/Primo/Search?lookfor=Wu%2C+Xingyong+&type=Author&filter%5B%5D=rtype%3A%22Articles%22&filter%5B%5D=topic%3A%22Diverse+Techniques%22&filter%5B%5D=topic%3A%22Crystallization+-+Methods%22&filter%5B%5D=topic%3A%22Fundamental+And+Applied+Biological+Sciences.+Psychology%22&dfApplied=1

Indirect Fluorescent Antibody Technique Medicine & Life Sciences 7% * Immunocompromised Host Medicine & Life Sciences 7% ... Patients were considered to be infected if one or more of the comparison techniques gave a positive result. Immunoblotting was ... Patients were considered to be infected if one or more of the comparison techniques gave a positive result. Immunoblotting was ... Patients were considered to be infected if one or more of the comparison techniques gave a positive result. Immunoblotting was ...

https://novaresearch.unl.pt/en/publications/performance-of-immunoblotting-in-diagnosis-of-visceral-leishmania

The antibodies do not exhibit non-specific binding to dissimilar NPs such as gold NPs or carbon nanotube s as demonstrated through custom-designed in vitro assays. (cdc.gov)
Anticardiolipin and plasma B lymphocyte stimulator (BLyS) were measured by enzyme-linked immunosorbent assay, antinuclear antibodies (ANAs) by indirect immunofluorescence, and 13 autoantibodies by bead-based assays. (elsevier.com)
Meridian Life Science offers a range of reagents that are suitable for multiple assay formats, including IgM and IgG antibody detection, rapid anti-IgM assays, and Immunofluorescence assays (IFA). (meridianbioscience.com)
Assays are commercially available for the detection of IgG, IgM, or both IgG and IgM antibodies. (meridianbioscience.com)
Antibody testing can complement molecular assays for detecting COVID-19. (who.int)

We evaluated the concurrence between lateral flow assay and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). (who.int)

3] T. Kumanomido, Y. Okuda, and Y. Akiyama, "Sudies on outbreak of equine influenza in Japan in 1971: early diagnosis by direct fluorescent antibody technique. (fujipress.jp)

IFA is a traditional laboratory technique that utilizes fluorescent dyes to identify the presence of antibodies bound to specific antigens. (meridianbioscience.com)

Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. (lookformedical.com)
Lateral flow assay and ELISA techniques gave consistent results for IgG/IgM antibody measurements towards spike and nucleocapsid proteins, suggesting that both methods can be used to detect COVID-19 where access to molecular test kits is difficult. (who.int)
The scope of this study encompasses an investigation of the markets cell and gene therapy tools such as GMP proteins, media, cell separation and activation reagents, viral and non-viral, cytokine release syndrome monitoring products, GMP antibodies, leukapheresis instrumentation, immunoassays (multiplex and singleplex) and bioreactors. (medgadget.com)
Other in silico methods that are routinely used in research laboratories include molecular modelling (a technique used to model or mimic the structure of molecules) and protein sequencing and its alignment (methods used to evaluate identities and similarities in the amino acid sequence of proteins) [25-28]. (biomedscis.com)

Platelets, lymphocytes, and megakaryocytes, specifically processed for the intracellular detection of antigens, were studied by indirect immunofluorescence techniques using monospecific antibodies directed against factor VUI-related antigen (F-VIII-RA). (elsevier.com)
Antibodies present in the recipient's serum may be directed against antigens in the donor product. (lookformedical.com)
Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. (lookformedical.com)

Antigen-antibody testing has little clinical effect in an emergency department setting, although it may help in recalcitrant or unclear cases. (medscape.com)
A blood test ( Legionella antibody serology) or urinary antigen tests for Legionella are 2 tests for diagnosis. (legionella.org)
Soluble antigen is added in excess allowing the IgM antibody-antigen reaction to occur in the absence of competing IgG. (meridianbioscience.com)
Finally, a labelled detection antibody is added that has specific reactivity against the antigen. (meridianbioscience.com)
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). (bvsalud.org)
The solid-phase antibody is reacted with a urine specimen, and the urine albumin-antigen complexes with the solid-phase antibody. (cdc.gov)
This so-referred to as multiplex immunoassay is based on a mix of bead subsets which might be each labelled with a novel mixture of inner fluorescent signal and antigen diabetes center [url=http://healthdept.sp.gov.lk/purchase/Forxiga/]purchase forxiga 10 mg with mastercard[/url]. (ehd.org)

In this work, the results of the immunoblot analysis were compared to those of parasitological examination (Giemsa-stained smears and/or parasite isolation in Novy, Nicolle, and MacNeal medium from bone marrow) and indirect immunofluorescence and counterimmunoelectrophoresis techniques. (unl.pt)

The scFv antibodies are engineered with a FLAG tag to allow for secondary detection using standard immunohistochemistry methods. (cdc.gov)

Non-specific protein-protein interactions are also of interest in the formulation of biotherapeutics such as monoclonal antibodies and other biologic drugs. (wyatt.com)
Monoclonal antibodies can be used to diagnose B hermsii infection. (medscape.com)

For most ToRCH organisms, the initial screening test is based on the detection of antibodies to the organism. (meridianbioscience.com)

Antibody-Coated Bacteria Test, Urinary" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
The presence or absence of antibody-coated bacteria in urine correlates with localization of urinary tract infection in the kidney or bladder, respectively. (ucdenver.edu)
This graph shows the total number of publications written about "Antibody-Coated Bacteria Test, Urinary" by people in this website by year, and whether "Antibody-Coated Bacteria Test, Urinary" was a major or minor topic of these publications. (ucdenver.edu)
Below are the most recent publications written about "Antibody-Coated Bacteria Test, Urinary" by people in Profiles. (ucdenver.edu)
A solid-phase fluorescent immunoassay for the measurement of human urinary albumin is described by Chavers et al. (cdc.gov)
Results of the fluorescent immunoassay (FIA) are reproducible, and the test is accurate and sensitive for the detection of human urinary albumin excretion. (cdc.gov)

During 2000-2013, samples from 468 animals showing clinical signs of rabies or to whom humans were exposed were submitted to the CVL for rabies testing by using the fluorescent antibody test ( 5 ). (cdc.gov)

Immunofluorescence techniques in diagnostic microbiology / edited by Joan M. B. Edwards, C. E. D. Taylor, A. H. Tomlinson. (who.int)

The fluorescent immunoassay is a non-competitive, double-antibody method for the determination of human albumin in urine. (cdc.gov)

ELISA detected IgM nucleocapsid (N) antibodies in 18 samples, and IgG (N) and IgG spike 1 (S1) antibodies in 31 and 29 samples, respectively. (who.int)

In this work, we have developed antibodies to NPs from a phage library containing approximately 2x109 unique single-chain variable fragment (scFv) antibodies each displayed monovalently on the gene III coat protein of a M13 filamentous phage. (cdc.gov)
Renowned as the #1 antibody event in the industry, this year's agenda boasts 15 dedicated topic streams, 3 unmissable training course add-on options and will bring together more than 700 of the antibody and protein community. (cshlpress.org)

A Legionella serum antibody titer of 1:128 or more is suggestive of the diagnosis. (medscape.com)
You did have Legionnaires' disease, but the antibody titer declined because the test was drawn late. (legionella.org)
However, the antibody titer often reaches its peak 3 - 6 months later, and the rest remains positive for years in a few patients. (legionella.org)

Subsequent screening, if required, is carried out using a monoclonal antibody-based immunofluorescent assay (IFA). (meridianbioscience.com)
The assay works by binding IgM antibodies in the patient's specimen to a solid phase coated with an anti-IgM capture antibody. (meridianbioscience.com)
The lateral flow assay detected immunoglobulin M (IgM) and G (IgG) antibodies in 35 and 37 study group samples. (who.int)
After washing and blocking with PBST+5% BSA, detection was performed using Human Anti-Nivolumab Antibody, clone AbD30255 (HCA299) at a concentration of 2 μg/ml and an HRP labeled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent ( BUF049A ) followed by QuantaBlu Fluorogenic Peroxidase Substrate. (bio-rad-antibodies.com)
After washing and blocking with PBST+5% BSA, detection was performed using Human Anti-Nivolumab Antibody, clone AbD30255 (HCA299) titrated to the given concentrations in PBST followed by an HRP labeled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent ( BUF049A ) and QuantaBlu Fluorogenic Peroxidase Substrate. (bio-rad-antibodies.com)
Detection was performed using HRP conjugated Human Anti-Nivolumab Antibody ( HCA301 ) at a concentration of 2 µg/ml in HISPEC Assay Diluent ( BUF049A ) and QuantaBlu Fluorogenic Peroxidase Substrate. (bio-rad-antibodies.com)

Fluorescent antibody technique for visualizing antibody-bacteria complexes in urine. (ucdenver.edu)
This equilibrium is concentration-dependent, so a fractionation technique such as SEC-MALS - with the accompanying time-dependent dilution - is not suitable for full and rigorous characterization of reversible complexes. (wyatt.com)
Among the most useful techniques for studying reversible complexes is composition-gradient light scattering ( CG-MALS ). (wyatt.com)

IMSEAR at SEARO: Rapid diagnosis of bacterial meningitis by the fluorescent-antibody (FA) technique. (who.int)
This study confirms that the humoral response in patients coinfected with HIV and Leishmania is much lower than that in immunocompetent patients and that the immunoblot method is a sensitive, noninvasive, and specific serological technique for the diagnosis of visceral leishmaniasis in immunocompromised patients. (unl.pt)

Discovery and characterization of antibodies that bind nanoparticle s. (cdc.gov)

Serological identification of Actinomyces using fluorescent antibody techniques. (microbiologyresearch.org)

The lab of co-author Dr. Robert Bast Jr., an expert in ovarian cancer and vice president for translational research at MD Anderson, inserted gel-bound carbon nanotubes into the ovaries of rodents to mimic the accumulations that are expected for nanotubes linked to special antibodies that recognize tumor cells. (news-medical.net)

Confocal and fluorescence microscopy are also common techniques used to detect fluorescent NPs, however their detection ability is often obscured by tissue autofluorescence and are limited to detecting fluorescent NPs. (cdc.gov)
These techniques may also be used to visualize spirochetes using a fluorescence microscope. (medscape.com)
The fluorescent probe 4,4-difluoro-5-(4-phenyl-1, 3-butadienyl)-4-bora-3a, 4a-diaza-s-indacene-3-undecanoic acidity (C11-BODIPY), found in the PLIR technique, modifies its fluorescence from reddish colored (FL2) to green (FL1)8,10 due to oxidation. (healthweblognews.info)

A fluorescent-antibody technique for the detection of enterotoxin-producing cells of Clostridium perfringens type A / Manuel J. Torres-Anjel, Hans P. Riemann, and Che C. Tsai. (who.int)
The new results suggested that antibody-nanotube probes could potentially detect tumors with as few as 100 ovarian cancer cells, which could make it a valuable tool for early detection. (news-medical.net)
[3] The underlying mechanism involves immunoglobulin E antibodies (IgE), part of the body's immune system, binding to an allergen and then to a receptor on mast cells or basophils where it triggers the release of inflammatory chemicals such as histamine . (mdwiki.org)

Diagnostics was performed by fluorescent antibody test (FAT) and RT-PCR using diagnostic kits of domestic production. (bvsalud.org)

Sputum examination may be supplemented by using a Legionella -specific fluorescent antibody. (medscape.com)

The antibody serology blood test still may be useful in diagnosing your illness. (legionella.org)
A ToRCH serologic test must detect both IgM and IgG antibodies to the ToRCH panel of infectious diseases (Toxo, Rubella, CMV and HSV). (meridianbioscience.com)
Therefore, we targeted to judge the result of test storage for the PLIR technique. (healthweblognews.info)

Conventional techniques employed to detect NPs in tissues such as transmission electron microscopy coupled with energy dispersive x-ray spectroscopy offer superior nanoscale resolution, however pose limitations due to the high cost of sample processing and limited sample analysis throughput. (cdc.gov)
The novel antibodies isolated when used in conjunction with other existing techniques for NP detection will comprise a powerful tool kit, and enable researchers to use them to detect NPs both in the environment and in a biological milieu. (cdc.gov)

An IgG antibody produced in the first few months following an initial infection has a lower avidity than an IgG antibody produced several months or years later. (meridianbioscience.com)
Consequently, low-avidity antibody can be used to specifically identify high-risk mothers with a primary infection. (meridianbioscience.com)
The first exposure of an individual without antibodies against the virus is called primary infection. (bvsalud.org)

We have developed NP binding antibodies to commercially important NPs including QDs and TiO2 NPs using phage display technology. (cdc.gov)

IgM is the immediate antibody that is produced once a human is exposed to a bacteria, virus or a toxin and disappears within 2 to 3 weeks. (meridianbioscience.com)
Antibody to human albumin is covalently attached to derivatized polyacrylamide beads. (cdc.gov)
A microtiter plate was coated overnight with Human Anti-Nivolumab Antibody, clone AbD30255 (HCA299) at a concentration of 1 µg/ml. (bio-rad-antibodies.com)
After washing and blocking with PBST+5% BSA, a pre-incubated mixture of nivolumab (0.3 µg/ml), spiked with increasing concentrations of Human Anti-Nivolumab Antibody, clone AbD30255 (HCA299) was added. (bio-rad-antibodies.com)

Doing a PhD in Virology, you'll become proficient in the laboratory with techniques including fluorescent antibody staining, polymerase chain reaction (PCR) and cell culture. (findaphd.com)

We used Deming regression to evaluate the antibody measurements. (who.int)

Sensitivity to experimental conditions is also explored and it is shown that alternate methods like optical traps provide an even better technique for biological agent detection. (silverchair.com)

Results of search for 'su:{Fluorescent antibody technique. (who.int)

Antibodies of these substances are usually weak and quite labile, stimulated by erythrocytes. (lookformedical.com)

Anatomy3

Organisms13

Diseases11

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes7

Information Science1

Health Care3

Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. (1/24343)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (2/24343)

Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast. (3/24343)

Arrestin function in G protein-coupled receptor endocytosis requires phosphoinositide binding. (4/24343)

Coupling of the cell cycle and myogenesis through the cyclin D1-dependent interaction of MyoD with cdk4. (5/24343)

Retinoic acid, but not arsenic trioxide, degrades the PLZF/RARalpha fusion protein, without inducing terminal differentiation or apoptosis, in a RA-therapy resistant t(11;17)(q23;q21) APL patient. (6/24343)

Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells. (7/24343)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (8/24343)

Fluorescent Antibody Technique

Virus Cultivation

Antibodies

Immune Sera

Fluorescent Antibody Technique, Direct

Culture Techniques

Immunodiffusion

Antigens

Immunoglobulin G

Cattle

Antibodies, Viral

Rabbits

Abortion, Veterinary

Fluorescent Antibody Technique, Indirect

Antibody Specificity

Antibodies, Monoclonal

Antibodies, Bacterial

Complement Fixation Tests

gamma-Globulins

Cross Reactions

Bronchiolitis, Viral

Antigen-Antibody Reactions

Cattle Diseases

Antigens, Viral

Immunologic Techniques

Immunoglobulin M

Methods

Antibody Formation

Rabies

Antibodies, Neutralizing

Immunoenzyme Techniques

Microscopy, Electron

Antibodies, Protozoan

Antibodies, Anti-Idiotypic

Rabies virus

Antibody Affinity

Epitopes

Neutralization Tests

Hemagglutination Tests

Binding Sites, Antibody

Agglutination Tests

Kidney

Evaluation Studies as Topic

Dog Diseases

HIV Antibodies

Staining and Labeling

Antigens, Bacterial

Sensitivity and Specificity

Lyssavirus

Antibodies, Neoplasm

Antibodies, Antinuclear

Serologic Tests

Sarcocystis

Dysentery

Enzyme-Linked Immunosorbent Assay

Legionnaires' Disease

Hemagglutination Inhibition Tests

Neospora

Sarcocystosis

Spirochaeta

Legionella

Seroepidemiologic Studies

Horse Diseases

Swine Diseases

Molecular Sequence Data

Dogs

Immunoglobulin A

Autoantibodies

Antibodies, Fungal

Microscopy, Fluorescence

Encephalomyelitis

Swine

Cell Line

Viruses

Goats

Horses

Coombs Test

Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. (1/24343)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (2/24343)

Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast. (3/24343)