Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Process of growing viruses in live animals, plants, or cultured cells.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Substances that are recognized by the immune system and induce an immune reaction.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Premature expulsion of the FETUS in animals.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Serum globulins that migrate to the gamma region (most positively charged) upon ELECTROPHORESIS. At one time, gamma-globulins came to be used as a synonym for immunoglobulins since most immunoglobulins are gamma globulins and conversely most gamma globulins are immunoglobulins. But since some immunoglobulins exhibit an alpha or beta electrophoretic mobility, that usage is in decline.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
An acute inflammatory disease of the lower RESPIRATORY TRACT, caused by paramyxoviruses, occurring primarily in infants and young children; the viruses most commonly implicated are PARAINFLUENZA VIRUS TYPE 3; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; and METAPNEUMOVIRUS.
The processes triggered by interactions of ANTIBODIES with their ANTIGENS.
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
Substances elaborated by viruses that have antigenic activity.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A series of steps taken in order to conduct research.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Acute VIRAL CNS INFECTION affecting mammals, including humans. It is caused by RABIES VIRUS and usually spread by contamination with virus-laden saliva of bites inflicted by rabid animals. Important animal vectors include the dog, cat, bat, fox, raccoon, skunk, and wolf.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.
Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.
The type species of LYSSAVIRUS causing rabies in humans and other animals. Transmission is mostly by animal bites through saliva. The virus is neurotropic multiplying in neurons and myotubes of vertebrates.
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Sites on an antigen that interact with specific antibodies.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
Local surface sites on antibodies which react with antigen determinant sites on antigens (EPITOPES.) They are formed from parts of the variable regions of FAB FRAGMENTS.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
Antibodies reactive with HIV ANTIGENS.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Substances elaborated by bacteria that have antigenic activity.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A genus of the family RHABDOVIRIDAE that includes RABIES VIRUS and other rabies-like viruses.
Immunoglobulins induced by antigens specific for tumors other than the normally occurring HISTOCOMPATIBILITY ANTIGENS.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
Diagnostic procedures involving immunoglobulin reactions.
A genus of protozoa found in reptiles, birds, and mammals, including humans. This heteroxenous parasite produces muscle cysts in intermediate hosts such as domestic herbivores (cattle, sheep, pigs) and rodents. Final hosts are predators such as dogs, cats, and man.
Acute inflammation of the intestine associated with infectious DIARRHEA of various etiologies, generally acquired by eating contaminated food containing TOXINS, BIOLOGICAL derived from BACTERIA or other microorganisms. Dysentery is characterized initially by watery FECES then by bloody mucoid stools. It is often associated with ABDOMINAL PAIN; FEVER; and DEHYDRATION.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
An acute, sometimes fatal, pneumonia-like bacterial infection characterized by high fever, malaise, muscle aches, respiratory disorders and headache. It is named for an outbreak at the 1976 Philadelphia convention of the American Legion.
Serologic tests in which a known quantity of antigen is added to the serum prior to the addition of a red cell suspension. Reaction result is expressed as the smallest amount of antigen which causes complete inhibition of hemagglutination.
A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother.
Infection of the striated muscle of mammals by parasites of the genus SARCOCYSTIS. Disease symptoms such as vomiting, diarrhea, muscle weakness, and paralysis are produced by sarcocystin, a toxin produced by the organism.
A genus of flexible, spiral rods found in hydrogen sulfide-containing mud, sewage, and polluted water. None of the species properly referred to in this genus are pathogenic.
Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
Diseases of domestic and wild horses of the species Equus caballus.
Diseases of domestic swine and of the wild boar of the genus Sus.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.
Immunoglobulins produced in a response to FUNGAL ANTIGENS.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A general term indicating inflammation of the BRAIN and SPINAL CORD, often used to indicate an infectious process, but also applicable to a variety of autoimmune and toxic-metabolic conditions. There is significant overlap regarding the usage of this term and ENCEPHALITIS in the literature.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Established cell cultures that have the potential to propagate indefinitely.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
A test to detect non-agglutinating ANTIBODIES against ERYTHROCYTES by use of anti-antibodies (the Coombs' reagent.) The direct test is applied to freshly drawn blood to detect antibody bound to circulating red cells. The indirect test is applied to serum to detect the presence of antibodies that can bind to red blood cells.

Tyrosine phosphorylation is required for actin-based motility of vaccinia but not Listeria or Shigella. (1/24343)

Studies of the actin-based motility of pathogens have provided important insights into the events occurring at the leading edge of motile cells [1] [2] [3]. To date, several actin-cytoskeleton-associated proteins have been implicated in the motility of Listeria or Shigella: vasodilator-stimulated phosphoprotein (VASP), vinculin and the actin-related protein complex of Arp2 and Arp3 [4] [5] [6] [7]. To further investigate the underlying mechanism of actin-tail assembly, we examined the localization of components of the actin cytoskeleton including Arp3, VASP, vinculin and zyxin during vaccinia, Listeria and Shigella infections. The most striking difference between the systems was that a phosphotyrosine signal was observed only at the site of vaccinia actin-tail assembly. Micro-injection experiments demonstrated that a phosphotyrosine protein plays an important role in vaccinia actin-tail formation. In addition, we observed a phosphotyrosine signal on clathrin-coated vesicles that have associated actin-tail-like structures and on endogenous vesicles in Xenopus egg extracts which are able to nucleate actin tails [8] [9]. Our observations indicate that a host phosphotyrosine protein is required for the nucleation of actin filaments by vaccinia and suggest that this phosphoprotein might be associated with cellular membranes that can nucleate actin.  (+info)

Plasma membrane recruitment of RalGDS is critical for Ras-dependent Ral activation. (2/24343)

In COS cells, Ral GDP dissociation stimulator (RalGDS)-induced Ral activation was stimulated by RasG12V or a Rap1/Ras chimera in which the N-terminal region of Rap1 was ligated to the C-terminal region of Ras but not by Rap1G12V or a Ras/Rap1 chimera in which the N-terminal region of Ras was ligated to the C-terminal region of Rap1, although RalGDS interacted with these small GTP-binding proteins. When RasG12V, Ral and the Rap1/Ras chimera were individually expressed in NIH3T3 cells, they localized to the plasma membrane. Rap1Q63E and the Ras/Rap1 chimera were detected in the perinuclear region. When RalGDS was expressed alone, it was abundant in the cytoplasm. When coexpressed with RasG12V or the Rap1/Ras chimera, RalGDS was detected at the plasma membrane, whereas when coexpressed with Rap1Q63E or the Ras/Rap1 chimera, RalGDS was observed in the perinuclear region. RalGDS which was targeted to the plasma membrane by the addition of Ras farnesylation site (RalGDS-CAAX) activated Ral in the absence of RasG12V. Although RalGDS did not stimulate the dissociation of GDP from Ral in the absence of the GTP-bound form of Ras in a reconstitution assay using the liposomes, RalGDS-CAAX could stimulate it without Ras. RasG12V activated Raf-1 when they were coexpressed in Sf9 cells, whereas RasG12V did not affect the RalGDS activity. These results indicate that Ras recruits RalGDS to the plasma membrane and that the translocated RalGDS induces the activation of Ral, but that Rap1 does not activate Ral due to distinct subcellular localization.  (+info)

Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast. (3/24343)

Cell division in a number of eukaryotes, including the fission yeast Schizosaccharomyces pombe, is achieved through a medially placed actomyosin-based contractile ring. Although several components of the actomyosin ring have been identified, the mechanisms regulating ring assembly are still not understood. Here, we show by biochemical and mutational studies that the S.pombe actomyosin ring component Cdc4p is a light chain associated with Myo2p, a myosin II heavy chain. Localization of Myo2p to the medial ring depended on Cdc4p function, whereas localization of Cdc4p at the division site was independent of Myo2p. Interestingly, the actin-binding and motor domains of Myo2p are not required for its accumulation at the division site although the motor activity of Myo2p is essential for assembly of a normal actomyosin ring. The initial assembly of Myo2p and Cdc4p at the division site requires a functional F-actin cytoskeleton. Once established, however, F-actin is not required for the maintenance of Cdc4p and Myo2p medial rings, suggesting that the attachment of Cdc4p and Myo2p to the division site involves proteins other than actin itself.  (+info)

Arrestin function in G protein-coupled receptor endocytosis requires phosphoinositide binding. (4/24343)

Internalization of agonist-activated G protein-coupled receptors is mediated by non-visual arrestins, which also bind to clathrin and are therefore thought to act as adaptors in the endocytosis process. Phosphoinositides have been implicated in the regulation of intracellular receptor trafficking, and are known to bind to other coat components including AP-2, AP180 and COPI coatomer. Given these observations, we explored the possibility that phosphoinositides play a role in arrestin's function as an adaptor. High-affinity binding sites for phosphoinositides in beta-arrestin (arrestin2) and arrestin3 (beta-arrestin2) were identified, and dissimilar effects of phosphoinositide and inositol phosphate on arrestin interactions with clathrin and receptor were characterized. Alteration of three basic residues in arrestin3 abolished phosphoinositide binding with complete retention of clathrin and receptor binding. Unlike native protein, upon agonist activation, this mutant arrestin3 expressed in COS1 cells neither supported beta2-adrenergic receptor internalization nor did it concentrate in coated pits, although it was recruited to the plasma membrane. These findings indicate that phosphoinositide binding plays a critical regulatory role in delivery of the receptor-arrestin complex to coated pits, perhaps by providing, with activated receptor, a multi-point attachment of arrestin to the plasma membrane.  (+info)

Coupling of the cell cycle and myogenesis through the cyclin D1-dependent interaction of MyoD with cdk4. (5/24343)

Proliferating myoblasts express the muscle determination factor, MyoD, throughout the cell cycle in the absence of differentiation. Here we show that a mitogen-sensitive mechanism, involving the direct interaction between MyoD and cdk4, restricts myoblast differentiation to cells that have entered into the G0 phase of the cell cycle under mitogen withdrawal. Interaction between MyoD and cdk4 disrupts MyoD DNA-binding, muscle-specific gene activation and myogenic conversion of 10T1/2 cells independently of cyclin D1 and the CAK activation of cdk4. Forced induction of cyclin D1 in myotubes results in the cytoplasmic to nuclear translocation of cdk4. The specific MyoD-cdk4 interaction in dividing myoblasts, coupled with the cyclin D1-dependent nuclear targeting of cdk4, suggests a mitogen-sensitive mechanism whereby cyclin D1 can regulate MyoD function and the onset of myogenesis by controlling the cellular location of cdk4 rather than the phosphorylation status of MyoD.  (+info)

Retinoic acid, but not arsenic trioxide, degrades the PLZF/RARalpha fusion protein, without inducing terminal differentiation or apoptosis, in a RA-therapy resistant t(11;17)(q23;q21) APL patient. (6/24343)

Primary blasts of a t(11;17)(q23;q21) acute promyelocytic leukaemia (APL) patient were analysed with respect to retinoic acid (RA) and arsenic trioxide (As2O3) sensitivity as well as PLZF/RARalpha status. Although RA induced partial monocytic differentiation ex vivo, but not in vivo, As203 failed to induce apoptosis in culture, contrasting with t(15;17) APL and arguing against the clinical use of As203 in t(11;17)(q23;q21) APL. Prior to cell culture, PLZF/RARalpha was found to exactly co-localize with PML onto PML nuclear bodies. However upon cell culture, it quickly shifted towards microspeckles, its localization found in transfection experiments. Arsenic trioxide, known to induce aggregation of PML nuclear bodies, left the microspeckled PLZF/RARalpha localization completely unaffected. RA treatment led to PLZF/RARalpha degradation. However, this complete PLZF/RARalpha degradation was not accompanied by differentiation or apoptosis, which could suggest a contribution of the reciprocal RARalpha/PLZF fusion product in leukaemogenesis or the existence of irreversible changes induced by the chimera.  (+info)

Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells. (7/24343)

The human granulocytic ehrlichiosis (HGE) agent resides and multiplies exclusively in cytoplasmic vacuoles of granulocytes. Double immunofluorescence labeling was used to characterize the nature of the HGE agent replicative inclusions and to compare them with inclusions containing the human monocytic ehrlichia, Ehrlichia chaffeensis, in HL-60 cells. Although both Ehrlichia spp. can coinfect HL-60 cells, they resided in separate inclusions. Inclusions of both Ehrlichia spp. were not labeled with either anti-lysosome-associated membrane protein 1 or anti-CD63. Accumulation of myeloperoxidase-positive granules were seen around HGE agent inclusions but not around E. chaffeensis inclusions. 3-(2, 4-Dinitroanilino)-3'-amino-N-methyldipropylamine and acridine orange were not localized to either inclusion type. Vacuolar-type H+-ATPase was not colocalized with HGE agent inclusions but was weakly colocalized with E. chaffeensis inclusions. E. chaffeensis inclusions were labeled with the transferrin receptor, early endosomal antigen 1, and rab5, but HGE agent inclusions were not. Some HGE agent and E. chaffeensis inclusions colocalized with major histocompatibility complex class I and II antigens. These two inclusions were not labeled for annexins I, II, IV, and VI; alpha-adaptin; clathrin heavy chain; or beta-coatomer protein. Vesicle-associated membrane protein 2 colocalized to both inclusions. The cation-independent mannose 6-phosphate receptor was not colocalized with either inclusion type. Endogenously synthesized sphingomyelin, from C6-NBD-ceramide, was not incorporated into either inclusion type. Brefeldin A did not affect the growth of either Ehrlichia sp. in HL-60 cells. These results suggest that the HGE agent resides in inclusions which are neither early nor late endosomes and does not fuse with lysosomes or Golgi-derived vesicles, while E. chaffeensis resides in an early endosomal compartment which accumulates the transferrin receptor.  (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (8/24343)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space.  (+info)

Species, Research Grants, Research Topics, Publications, Genomes and Genes, Scientific Experts about indirect fluorescent antibody technique
Cloyd, M W.; Bolognesi, D P.; and Bigner, D D., Immunofluorescent analysis of expression of the rna tumor virus major glycoprotein, gp71, on surfaces of virus- -producing murine and other mammalian species cell lines. (1977). Subject Strain Bibliography 1977. 1745 ...
Define indirect fluorescent antibody. indirect fluorescent antibody synonyms, indirect fluorescent antibody pronunciation, indirect fluorescent antibody translation, English dictionary definition of indirect fluorescent antibody. adj. 1. Diverging from a direct course; roundabout. 2. a. Not proceeding straight to the point or object. b. Not forthright and candid; devious. 3.
Immunofluorescent analysis of Heat Shock Protein 84 using Anti-Heat Shock Protein 84 Polyclonal Antibody (Product# PA3-012) shows staining in U251 Cells. Heat Shock Protein 84 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 84 (Product# PA3-012) at a dilution of 1:100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552, Goat Anti-Rabbit). Images were taken at 60X magnification. ...
A glycoprotein with an apparent 340,000 mol wt (gp 340K) was isolated from rat kidney saline-soluble extract by ammonium sulfate precipitation, DE 52 ion-exchange cellulose chromatography, concanavalin A affinity column, Sephacryl S-300 gel filtration, and discontinuous polyacrylamide gel electrophoresis (PAGE). The relative purity of gp 340K was examined by double immunodiffusion analysis, disc PAGE, and immunoelectrophoresis. Injection of rabbit gp 340K antiserum into pregnant rats during the organogenetic period induced abnormal embryonic development, fetal growth retardation, and embryonic death. Antiserum against the immunocomplexes isolated by immobilized protein A also produced the same embryotoxic effects. The biologic effects of the antisera appeared to be dose dependent. Defects such as anophthalmia, hydrocephaly, exencephaly, cleft palate, cleft lip, and some cardiovascular anomalies were observed. The most frequently observed anomaly was anophthalmia. Immunofluorescent localization ...
Dynein tethers centrosomes to spindle poles. (a) Cytoplasmic dynein is eluted from spindles by addition of mAb 70.1. Immunofluorescent localization of dynei
Learning Objectives Describe the benefits of immunofluorescent antibody assays in comparison to nonfluorescent assays Compare direct and indirect flu
Dive into the research topics of An immunofluorescent study of the distribution of fibronectin and laminin during limb regeneration in the adult newt. Together they form a unique fingerprint. ...
Immunofluorescence localization and immunoblotting of Gαs in oocytes in Gpr3+/+ and Gpr3−/− ovaries. (A and B) Gαs in Gpr3+/+ (A) and Gpr3−/− (B) oocy
A monoclonal antibody, designated C138, was raised against a particulate fraction from young postnatal mouse cerebellum. In sections from adult mouse brain and cerebellum, this antibody stained a sub-population of neurons. Cell bodies of large neurones and fibre tracts were negative in all brain regions examined, whereas neuropil was heavily labelled. Immune-electron microscopy confirmed the specificity of the antibody for certain types of neurones and indicated that the antigen may be associated with microtubular structures. Immunofluorescence studies on dissociated cerebellar cultures showed that the antigen was expressed inside all tetanus toxin-positive neurones but not by non-neuronal cells.
Lab Reagents. Fluorescent Laboratories manufactures the fluorescent antibody reagents distributed by Genprice. The Fluorescent Antibody reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Fluorescent. Other Fluorescent products are available in stock. Specificity: Fluorescent Category: Antibody. Lab Tools information ...
Facilities for diagnosis of rabies through RT-PCR, mice-inoculation, cultural isolation and, fluorescent antibody technique have been created and is being used on a regular basis. ...
Tojasida Mol Talk combines the advantages of an easy to learn dinamicx programmable procedural scripting with the flexibility and power of a full programming language. Repeatability was also examined to ensure the reliability of the preparation method. It is designed for easy and rapid image manipulation and display of protein molecules, and is intended for users who need to quickly produce high-resolution images of protein molecules but do not have the time or inclination to use a software molecular visualization system.. This report describes the results for a bilateral comparison of ammonia in nitrogen gas mixture. Double-label immunofluorescence observations on spread BHK cells using this monoclonal antibody and a rabbit polyclonal antibody directed against the 54, and 55, mol -wt proteins showed that themol -wt species co-distributed with IF in a fibrous pattern.. Secondly, the authors do not discuss limitations of their approaches in the discussion. Asco operations staff during all modes ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
MACS® GMP CD34 Fluorescent Antibodies consist of a mouse monoclonal antibody against the human CD34 antigen conjugated to a fluorochrome. They are developed for flow cytometric identification and flow cytometric sorting of CD34-positive cells. | Principat dAndorra
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
To 2 h TR-OS challenge and remained from the media for your period from the analyze. Cells were being imaged and codistribution analyzed utilizing a binary submask. c M5 (MREG knockdown) and M5 cells transfected with MREG, (these cells are specified (R)) 108341-18-0 Purity & Documentation challenged with TR-OS for 1 h were being fastened, stained for LC3 and MREG. Cells were being imaged and codistribution analyzed working with a binary submask Pearsons coefficient 0.sixty four. The impression quantitation info in this particular figure is really an common of forty cells for every industry, with ten L-Cysteine (hydrochloride) Technical Information fields analyzed in two independent experiments. Mistake bars signify EM (p0.005). C2 details plotted is from visuals demonstrated in Fig. 2. Western blot assessment exhibiting MREG concentrations in C2, M5, and MREG rescue experiments (S. Fig. 3)Creator Manuscript Creator ManuscriptMol Neurobiol. Writer manuscript; 341031-54-7 Formula obtainable in ...
MADRID - Results from the final stage of a nationwide antibody study showed some 5.2% of Spains population has been exposed to the cor...
Fluorescent controls are useful for visual indication of delivery, and when combined with functional knockdown assessment, provide a useful tool for optimization.. siGLO Positive controls will effectively silence the indicated gene, and result in punctate cytoplasmic fluorescence. All are labeled with DY-547 (Cy3 analog). siGLO RISC-Free Control is a DY-547-labeled negative control that can also be co-transfected with functional siRNAs.. siGLO Transfection Indicators are unique non-RISC engaging molecules that localize to the nucleus, providing a distinct visual indication of transfection success. They are available with either DY-547 (Red) or 6-FAM (Green).. ...
Sino Biological offers some good and bad immunofluorescence results of some targeted proteins expressed in the cytoplasm of SHSY5Y cells.
Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye. We use the indirect immunofluorescence staining to perform cells fixed on slides and examine under a fluorescence microscope. - Immunofluorescence - AbVideo™ - Support - Abnova
The cellular localization of gastrin in the rabbit pyloric antrum was established by immunofluorescence. The gastrin cell was argyrophil (Grimelius technique) and identical with a previously described cell type that emits fluorescence upon combined formaldehydeozone treatment, a feature that has been interpreted as indicating storage of peptides with NH2-terminal tryptophan. The identity of the peptides and its relation to gastrin is unknown.
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Some laboratories offer direct immunofluorescent tests on fresh specimens, but these tests are labor- and personnel-intensive and are less sensitive than culture methods. In order to overcome the expe... more
Kajstura J, Bereiter-Hahn J. Scanning microfluorometric measurement of immunofluorescently labelled microtubules in cultured cells. Dependence of microtubule content on cell density. Histochemistry. 1987; 88(1):53-5 ...
Today is exciting because I get to do two things. 1: Post the first successful images of West Nile virus infectined cells that I took on my own and 2: Give a basic explanation one of the most visually impressive techniques at our disposal: immunofluorescent microscopy. For those of you not familiar with fluorescent microscopy…
TY - JOUR. T1 - Fine structure and immunofluorescent studies of the wish cell line. AU - Meek, William D.. AU - Davis, Walter L.. PY - 1986/12/1. Y1 - 1986/12/1. N2 - Morphological studies of the WISH cell line reveal an epithelioid cell type with some characteristics of both the original human amnion epithelium and a transformed state. WISH cells have a cytoplasm filled with microtubules; however, actin filament bundles are few, with actin localized at areas of cell contact and arranged diffusely through the cytoplasm, as viewed by indirect immunofluorescence. Fingerlike projections or short filopodia are observed connecting cells that grow in a closely apposed monolayer. Other surface features, as viewed by scanning electron microscopy, include microvilli and blebs. Transmission electron microscopy shows that WISH cultures consist of light or dark cells with organelles that include lipid droplets, abundant free ribosomes, tubular mitochondria, lysosomes, annulate lamellae, rough endoplasmic ...
BD Difco™ Fluorescent Antibody Reagents FA Bordetella Pertussis; 5mL BD Difco™ Fluorescent Antibody Reagents Antibody Binding Proteins and...
This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.. ...
As with most fluorescence techniques, a significant problem with immunofluorescence is photobleaching. Loss of activity caused by photobleaching can be controlled by reducing or limiting the intensity or time-span of light exposure, by increasing the concentration of fluorophores, or by employing more robust fluorophores that are less prone to bleaching (e.g., Alexa Fluors, Seta Fluors, or DyLight Fluors). Some problems that may arise from this technique include autofluorescence, extraneous undesired specific fluorescence, and nonspecific fluorescence. Autofluorescence includes fluorescence emitted from the sample tissue or cell itself. Extraneous undesired specific fluorescence occurs when a targeted antigen is impure and contains antigenic contaminants. Nonspecific fluorescence involves the loss of a probes specificity due to fluorophore, from improper fixation, or from a dried out specimen.[3]. Immunofluorescence is only limited to fixed (i.e., dead) cells when structures within the cell are ...
The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. If quantitative results are required titration is necessary. Such titrations may vary greatly between different laboratories. The present study involving 26 laboratories shows an improvement of interlaboratory comparability for the homogeneous fluorescence pattern when a common reference serum is used. Cultured cells as substrate appear to give better quantitative agreement than rat liver sections. National reference sera should be standardised in items of the appropriate WHO reference preparation. ...
Immunofluorescence procedures for SNs and rat cortical neurons followed those of Chin et al. (1999). Briefly, cells were fixed in a solution of 4% paraformaldehyde in PBS containing 20% sucrose. After three rinses in PBS, fixed cells were blocked for 30 min at room temperature in Superblock buffer (Pierce), 0.2% Triton X-100, and 3% normal goat serum and subsequently incubated overnight at 4°C with anti-phosphorylated ERK (pERK) antibody (1:500), anti-phosphorylated p38 (p-p38) MAPK antibody (1:200), anti-MAPK phosphatase 1 (MKP1) antibody (1:500), or anti-pCREB2 antibody (1:500). Anti-pERK antibody was purchased from Cell Signaling Technology. Anti-p-p38 MAPK and anti-MKP1 antibodies were purchased from Santa Cruz Biotechnology. The anti-pCREB2 antibody was raised by a commercial vendor (Genemed Biotechnologies) against the phosphorylated version of a CREB2 hybrid peptide (SPPDSPEQGPSSPET) constructed to juxtapose the sequences immediately surrounding two putative MAPK phosphorylation sites ...
We recently teamed-up with Virology Research Services (VRS) to test a large panel of viral antibodies in immunofluorescence applications. This work was funded by the Medical Research Council (Proximity to Discovery Award for Knowledge Exchange) through the University College London Translational Research Office and aimed to improve utilisation of the available immunofluorescence resources.
KPL DyLight conjugates offer a brilliant choice in a variety of immunofluorescence detection applications. They combine the sensitivity and reproducibility of our affinity purified secondary antibodies with a series of DyLight dyes that span the light spectra from visible to infrared. They are brighter than fluorescein, rhodamine, Cy™3 and Cy5 and offer comparable brightness and photostability to Alexa conjugates. They are ideal for use in the following applications:. • Fluorescent Western ...
Fig. 2- Representative images for the dual immunohistochemical and immunofluorescence detection of CD4+FOXP+ T-regulatory cells within human lung tissue. Cell nuclei were counterstained with either a-f) 4,6-diamidino-2-phenylindole (blue stain) or g and h) Mayers haematoxylin (blue stain). a) CD4+ cells were identified using an Alexa-488 conjugated goat anti-mouse secondary antibody (red stain) and FOXP3+ cells were detected using 3,3′-diaminobenzidine following indirect immunohistochemistry (brown stain). b) Increased magnification of the highlighted area in (a) demonstrating the distinct nuclear staining of FOXP3 (brown stain) in cells that are also CD4+ (red stain, indicated by arrow). c) A single CD4+FOXP3+ cell (arrow) detected within an airway submucosa. d) A CD4+FOXP3+ cell detected within a population of CD4+FOXP3- T-cells within an alveolar septum. e) A lymphoid follicle expressing an abundance of CD62L throughout with a few CD62L+FOXP3+ cells (arrow). f) Sub-epithelial FOXP3+ cell ...
Carboxypeptidase O (CPO), a member of the M14 family of proteolytic enzymes, preferentially cleaves C-terminal acidic amino acids, with weak affinity towards hydrophobic amino acids. We investigated the subcellular localization of CPO, and after immunofluorescent analysis of stably-transfected MDCK cells, we found that CPO co-localized with calnexin, an ER marker. To determine what CPO does in the ER, MDCK cells were transfected with plasmids expressing Gaussia Luciferase (GLuc) containing a C-terminal ER retention signal (KDEL). Previous experiments suggested that CPO cleaves the KDEL sequence of GLuc, causing its secretion. In ongoing experiments, plasmids expressing GLuc tagged with modified KDEL sequences (KDELD, KDELE, and KDELEL) will be transfected, and the intracellular activity of CPO against these substrates will be assessed.
It is well known that this reagent leads to a depletion of extracellular Ca2+ which in turn causes a disassembly of tight junction [1, 2]. The latter is reflected by a significant drop in the TER readings. Subsequent replacement of the EGTA containing medium by standard medium led to a regeneration of the tight junctions network as revealed by increasing TER readings. For validation of the temporary break down of the barrier function two cell cultures were fixed just before removal of EGTA. Samples were then stained for immunofluorescent analysis of cell nuclei and ZO-1 proteins. Imaging by Confocal Laser Scanning Microscopy and comparison with the untreated reference cell culture clearly revealed the disintegration of the tight junctions network induced by EGTA exposure. These findings are in excellent agreement with the TER results and demonstrate the benefits of using a label-free and noninvasive technique as implemented in the cellZscope.. Cell layer formation. Application of impedance ...
Freshly isolated peripheral blood mononuclear cells (PBMC) from 10 healthy volunteers, 28 patients with rheumatoid arthritis (RA), eight patients with osteoarthritis, and five patients with ankylosing spondylitis were examined for interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) production using monoclonal antibodies and an indirect immunofluorescent method. In freshly isolated PBMC from healthy controls very few cells were stained for either IL-1 type. All 20 RA patients who were not receiving parenteral gold therapy had PBMC staining for IL-1 alpha. In these patients, up to 7.5% of PBMC showed bright IL-1 alpha staining (range 1.2-7.5%). No IL-1 beta staining was seen. These IL-1 alpha-staining cells had a dendritic morphology and the percentage of cells staining correlated well with levels of C-reactive protein, an index of disease activity in these RA patients. Significantly fewer IL-1 alpha-staining cells were present in the peripheral blood of RA patients receiving gold therapy
Spa2p and Cdc10p both participate in bud site selection and cell morphogenesis in yeast, and spa2delta cdc10-10 cells are inviable. To identify additional components important for these processes in yeast, a colony-sectoring assay was used to isolate high-copy suppressors of the spa2delda cdc10-10 lethality. One such gene, AXL2, has been characterized in detail. axl2 cells are defective in bud site selection in haploid cells and bud in a bipolar fashion. Genetic analysis indicates that AXL2 falls into the same epistasis group as BUD3. Axl2p is predicted to be a type I transmembrane protein. Tunicamycin treatment experiments, biochemical fractionation and extraction experiments, and proteinase K protection experiments collectively indicate that Axl2p is an integral membrane glycoprotein at the plasma membrane. Indirect immunofluorescence experiments using either Axl2p tagged with three copies of a hemagglutinin epitope or high-copy AXL2 and anti-Axl2p antibodies reveal a unique localization ...
Intermediate Filaments;Sodium Dodecyl Sulfate;Antibodies;Retinitis Pigmentosa;Fluorescent Antibody Technique, Indirect;Microscopy, Fluorescence;Electrophoresis, Polyacrylamide Gel;Spinal ...
Immunofluorescence localization was performed as previously described.31 Briefly, corneas were fixed with cold methanol or 4% paraformaldehyde in PBS, cryoprotected with sucrose-PBS in a series of dilutions (10%, 20%, and then 30%), embedded and frozen in OCT medium (Sakura Finetek, Torrance, CA, USA). Cross sections of 6 μm were cut using a cryostat (Microm HM 505E; GMI, Ramsey, MN, USA) followed by immunofluorescence localization. For corneal flat mounts, freshly enucleated corneas were removed under an operating microscope (Carl Zeiss Microscopy, Oberkochen, Germany). Cornea flat mounts were digested in 1 mg/mL collagenase solution (Worthington Biochemical Corporation, Lakewood, NJ, USA) in PBS for 7 minutes at 37°C to facilitate antibody penetration into the tissue. No collagenase was used for BrdU or Ki-67 flat mounts which were fixed in cold methanol. For BrdU staining, tissue was treated with 2N HCl at 37°C for 15 minutes to denature DNA and neutralized in boric acid (pH 8.5) 3 times ...
Methods and Materials This review targets the role of antibody methods and sialylation because of its quantitation. this lectin-affinity small fraction holds the entire anti-inflammatory activity, with the nonbinding fraction being essentially ineffective. At first glance, these results appear to match nicely with the 11% of sialylated N-glycans found in the Fc region [8, 22]. The situation, however, is more complex. Site-specific analysis of the SNA binding and nonbinding fractions of IVIG revealed no significant difference in Fc sialylation [22]. The obvious conclusion was that the fractionation of IgG on SNA was solely based on the N-glycans in the variable domains, whereas the sialoglycans in the CH2 domain name were inaccessible to the lectin. This view was seemingly supported by SNA fractionation of isolated Fab fragments [22]. However, it harshly contradicts the earlier conclusion that this anti-inflammatory activity depended on sialylation of the Fc region N-glycans [3, 4]. Stadlmann and ...
You can use an immunoassay like a homogeneous sandwich FRET assay: You can use donor-dye-labelled fluorophore antibodies that are able to recognise specific antigens on the virus to bind the virus antigen, then introduce a second set of antibodies labelled with acceptor-dye fluorophore. If the vaccine works and produces an immune response in the in-vivo model that destroys the virus, the antigen that joins the donor-labelled and acceptor-labelled antibodies is also destroyed and can no longer sandwich the two antibodies close together within the Förster distance to exhibit FRET.. ...
The culture of African green monkey kidney fibroblasts that appears in this section was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Rhodamine Red-X.
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
The culture of sheep kidney cells (MDOK line) illustrated in this section was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to fluorescein.
A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dependable means of measuring serum antibody to B. burgdorferi.
A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dependable means of measuring serum antibody to B. burgdorferi. ...
TY - JOUR. T1 - Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method. AU - Noda, N.. AU - Ikuta, H.. AU - Ebie, Y.. AU - Hirata, A.. AU - Tsuneda, Satoshi. AU - Matsumura, M.. AU - Sumino, T.. AU - Inamori, Y.. PY - 2000. Y1 - 2000. N2 - Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed bacterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO 14298) and sixteen against Nitrobacter winogradskyi (IFO 14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and ...
Figure 3. Immunofluorescent localization of procollagen I and Hsp47 in the presence or absence of brefeldin A treatment in CEC. Cells were treated with 2 µg/ml brefeldin A for 30 min, fixed, permeabilized, and stained as described in the text. A. Procollagen I (green) and Hsp47 (red) in brefeldin A-treated cells. B. Prolyl 4-hydroxylase (green) and procollagen I (red) in brefeldin A-treated cells. C. Prolyl 4-hydroxylase (green) and Hsp47 (red) in brefeldin A-treated cells. D. Phase-contrast microscopy of C. Bar, 10 µm.. ...
Parafusin (PFUS), a superfamily member of phosphoglucomutase (PGM), is involved in the exocytic nano-machinery. It is a 63kD phosphoglycoprotein with two cyclic covalent modifications (phosphorylation/dephosphorylation and phosphoglucosylation/dephosphoglucosylation). It is a component of the scaffold associated with dense core secretory vesicles (DCSV). This thesis focuses on the role of PFUS and its post-translational modifications in exocytosis.;To conclusively establish the importance of PFUS in exocytosis, PFUS RNAi was performed in Paramecium by bacteria feeding. Cells exposed to RNAi showed loss of exocytosis (60-70%) after 3-4 generations of growth (72 hr). Using pan-PGM and PFUS antibodies on western blots, PFUS was shown to be specifically knocked-down without obvious change in overall PFUS/PGM level. Immunofluorescent localization in RNAi cells showed reduction of PFUS synthesis and number of DCSVs, indicating PFUS affects the secretory pathway. These cells recover exocytosis capacity ...
A procedure has been developed for the determination of the concentration of infective Newcastle disease virus (NDV) based on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. Infective virus assayed by the fluorescent cell-counting procedure is expressed in terms of cell-infecting units (CIU).. Adsorption of NDV to HeLa cell monolayers reached a plateau 1 to 1.5 hours after inoculation of coverslip cultures, and 12 per cent of the infective particles inoculated failed to adsorb. The half-life of NDV in protein-free Eagles medium at 37°C. was 2.1 hours. There was a linear relationship between virus concentration and the number of infected cells. The coefficient of variation of the mean of replicate determinations of infective NDV was 8.2 per cent. The distribution of single infected HeLa cells in the monolayer corresponded to the Poisson distribution. With NDV the cell-infecting unit (CIU) determined in HeLa cells is ...
The SHV-1 β-lactamase gene was sub-cloned into the pBC SK(-) vector (Stratagene, LaJolla, CA) from a clinical strain of K. pneumoniae (15571), and transformed into E. coli DH10B cells (Invitrogen, Carlsbad, CA) [15]. The K. pneumoniae clinical isolate possessed the SHV-5 ESBL and was obtained from a previous study [16]. E. coli DH10B without the blaSHV-1 gene served as a negative control.. The procedures used to isolate, express and purify the SHV-1 β-lactamase and to produce the anti-SHV β-lactamase antibodies have been previously detailed [13]. Purified anti-SHV antibodies were fluorescein-labeled with the EZ-Label™ fluorescent labeling kit (Pierce, Rockford, IL), according to the instructions of the manufacturer. In brief, 1 mg of polyclonal anti-SHV antibodies in 1 ml phosphate buffered saline (PBS, 2 mM monobasic sodium phosphate, 8 mM dibasic sodium phosphate, 154 mM sodium chloride, pH 7.4) was mixed with 7.6 μl of a 10 mg/ml solution of NHS-fluorescein in N, N-dimethylformamide for ...
Background Hypertonic saline (HS) continues to be successfully utilized clinically for treatment of varied types of cerebral edema. using Traditional western blotting, dual immunofluorescence and real-time RT-PCR, as well as the model also was useful for evaluation of mind drinking water content material (BWC) and infarct size. SB203580 and SP600125, particular inhibitors from the p38 and JNK signaling pathways, had been used to take care of primary microglia ethnicities to determine if the two signaling pathways had been necessary for the inhibition of HS on microglia expressing and secreting TNF- and IL-1 using Traditional western blotting, dual immunofluorescence and enzyme-linked immunosorbent assay (ELISA). The result of TNF- and IL-1 on NKCC1 manifestation in major astrocyte ethnicities was determined. Furthermore, the immediate inhibitory aftereffect of HS on NKCC1 manifestation in major astrocytes was also looked into by Traditional western blotting, dual immunofluorescence and real-time ...
Single-cell analysis reveals aspects of cellular physiology not evident from population-based studies, particularly in the case of highly multiplexed methods such as mass cytometry (CyTOF) able to correlate the levels of multiple signaling, differentiation and cell fate markers. Immunofluorescence (IF) microscopy adds information on cell morphology and the microenvironment that are not obtained using flow-based techniques, but the multiplicity of conventional IF is limited. This has motivated development of imaging methods that require specialized instrumentation, exotic reagents or proprietary protocols that are difficult to reproduce in most laboratories. Here we report a public-domain method for achieving high multiplicity single-cell IF using cyclic immunofluorescence (CycIF), a simple and versatile procedure in which four-color staining alternates with chemical inactivation of fluorophores to progressively build a multichannel image. Because CycIF uses standard reagents and instrumentation ...
Summary Fluorescent antibody technics were used to determine the localization and distribution of Schistosoma mansoni antigen in tissue cells, the presence of circulating antibody, and the sites of antibody production or in vivo antigen-antibody combination. The experiments were performed in mice after a primary infection with cercariae, after several challenges, and after antigen stimulation and challenge. Evidence of the presence of circulating antibody was first observed at 20 days in the inhibition fluorescent antibody test, at 25 days in the indirect fluorescent antibody test, at 42 days with the cercarial fluorescent antibody test, and at 47 days in the Cercarienhullen reaction. Sites of antibody production or in vivo antigen-antibody combination were observed in inflammatory cells of the portal tracts, especially those along the smaller arteries, and in granulomas and isolated cells in the parenchyma of the liver; in the perivascular tissue cells, isolated parenchymal cells and granulomas of the
Synonyms for direct fluorescein-conjugated antibody in Free Thesaurus. Antonyms for direct fluorescein-conjugated antibody. 21 words related to antibody: active site, protein, autoantibody, precipitin, ABO antibodies, Rh antibody, antitoxin, agglutinin, Forssman antibody.... What are synonyms for direct fluorescein-conjugated antibody?
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
BioAssay record AID 332145 submitted by ChEMBL: Induction of cellular microtubule disrupting activity in rat A10 cells at 1 ug/ml after 24 hrs by indirect immunofluorescence technique.
We wished to determine whether CD4+ T cells could reject a skin graft that was discordant for a single minor transplantation Ag in the absence of CD8+ T cells or Ab. Transgenic A1(M) mice were constructed that express the rearranged V beta 8.2 and V alpha 10 TCR genes from a T cell clone that is specific for the male Ag (H-Y) in the context of H2-Ek. In addition, the RAG-1(-/-) background was bred onto these mice to eliminate any endogenous TCR rearrangements. As expected, clonal deletion was found to be complete in the thymus of male A1(M) x RAG-1(-/-) mice, while only CD4+ T cells were positively selected and found in the periphery of females. Female A1(M) x RAG-1(-/-) mice were able to rapidly reject (in |14 days) male (but not female) skin grafts in a CD4-dependent fashion. After multiple grafts, it was confirmed that no CD8+ T cells or surface Ig+ B cells were present. An immunofluorescent analysis of spleen cells after grafting showed that the majority of T cells expressed activation markers (CD44
In the present study, we examined AQP4 function in reactive astrocytes compared between wild-type (WT) and AQP4-deficient (AQP4/KO) mice after a stab wound to the cerebral cortex. To examine activity of astrocytes, proliferating cells were labeled with 5-bromo-2-deoxyuridine (BrdU) incorporated in the drinking water provided to mice. By the immunofluorescent analysis using anti-BrdU antibody, astrocyte reactivity was at high level around the lesion site for WT mice 3 days after the stab wound to the brain, while it was much less for AQP4/KO mice. To identify the molecules related in injured mouse brain, we performed microarray analysis and found that more than 400 genes around the lesion site were upregulated 3 days after the wounding in WT mice ...
Efficient cleavage and probe versatility for highly-multiplex detection and co-localization. Modified Lightning Terminators™ provide a robust method for detecting multiple antigens from a single sample. Immunofluorescent detection of antigens in fixed tissue specimens and on immunoblots is used routinely in clinical practice and research laboratories, but current methods are limited to the detection of one to four antigens per tissue section or blot. Consequently, the detection of additional antigens requires multiple independent stains on separate sections and limits the co-localization of antigens on a single section. Whether in immunohistochemistry or immunoblotting, our photocleavable fluorescent labels (PCLs) provide advantages over current methods.. Highly Serial and Multiplex Detection. PCL technology uses our expertise in photocleavable chemistry to provide structures that can both efficiently bind to target antigens and rapidly cleave the dye reporter after exposure to UV light. The ...
Animals, Antilymphocyte Serum, Cell Separation, Complement System Proteins, Fluorescent Antibody Technique, Genetic Linkage, Isoantigens, Killer Cells, Natural, Male, Mice, Mice, Inbred A, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Inbred DBA, Mice, Inbred NZB. ...
Recent investigations have shown the feasibility of applying the fluorescent antibody method to Lancefield grouping of streptococci.1-4 The present study was un
Buy Goat Anti-Rabbit IgG (H+L chain specific) secondary antibody (MBS674759) product datasheet at MyBioSource, Secondary Antibodies. Application: ELISA; Immunoblot; Immunofluorescence; Immunohistochemistry
Cy3 Conjugated Anti-Mouse IgG SABC Kit is for indirect sensitive immunofluorescent detection of proteins in ICC or IHC via StreptAvidin-Biotin Complex (SABC) method.
Confocal immunofluorescent detection of wingless protein (green), engrailed protein (blue), and enhancer trap 5953 (red) in a reiterated segment of embryonic Drosophila neuroectoderm (ventral midline, center; anterior, top). The wingless protein nonautonomously activates 5953 expression, maintains expression of the engrailed protein, and controls the formation and specification of central nervous system precursors. See page 1594. [Photo: Chris Q. Doe] ...
Background Bcl-2 (B cell lymphoma/leukemia gene-2) may be the 1st proto-oncogene proven to function by inhibiting programmed cell loss of life/apoptosis. immunofluoresence and blot staining were employed to testify the marker protein of both mesenchymal and epithelial cells. Outcomes Unexpectedly, a dramatic modification of phenotype from epithelial cells to fibroblast-like cells was seen in Bcl-2-transfected cells. Traditional western blot immunofluoresence and evaluation MS-275 staining outcomes proven how the E-cadherin and desmoplakin, markers of epithelial cells, had MS-275 been downregulated in the Bcl-2-transfected cells. Nevertheless, Vimentin and N-cadherin, markers of mesenchymal cells, had been upregulated in these cells. Redistributions of cytokeratin and beta-catenin were seen in the Bcl-2-transfected cells also. Our outcomes demonstrated how the Bcl-2-transfected MCF10ATG3B cells maintained some epithelial markers additional, such as for example epithelial particular antigen (ESA) ...
annotations (the reliablity of the annotated protein expression using immunohistochemically (IH) stained on human tissues, the reliablity of the annotated protein expression in immunofluorescently (IF) stained human cell lines, tissue specificity (the distribution of antibody staining or protein expression in human cell types), cell line specificity (the distribution of RNA abundance in cell lines) and subcellular location (based on immunofluorescent staining of cell lines ...
[button size=small text=MSDS & Datasheet link=/wp-content/uploads/media/BCDatasheets_C_10.26/AGK/AGK-007-2.pdf]Pure Goat Anti-Rabbit IgG Gel, Spe
Creative Diagnostics provides DiagNano™ Goat Anti-Rabbit IgG, QD 655 nm conjugate for immunoassay, bioseparation and medical imaging applications.
Main Page , Multiple Labeling. It is often useful to be able to stain for two or more antigens in one common tissue section. This can be achieved by immunofluorescence method using different fluorescent dyes. Multiple staining can also be done with peroxidase conjugated antibodies developed with different chromogen substrates to produce the end products of different colors. There are three basic approaches in planning multiple staining: parallel, sequential and adjacent. In addition, the antibody dilution and condition are also important factors to be considered. Finally, appropriate color combination is also crucial since improper color combination may produce poor result and fail to demonstrate multiple antigens in the same section. For best result, the careful design and test of multiple staining protocols are necessary.. ...
The Gibbs laboratory is focused on the development of novel fluorophores and fluorescence imaging technologies to improve cancer detection and treatment. The main research focuses of the group are on fluorophore development for image-guided surgery, fluorescent labeling of small molecule therapeutics to improve understanding of effective cancer therapy and development of highly multiplexed immunofluorescence imaging technologies to permit deep multiplexed imaging on tissues. Current projects in the Gibbs laboratory include (1) development of near infrared nerve-specific fluorophore to improve nerve sparing during radical prostatectomy, (2) contrast agent and imaging methodology development to improve breast cancer margin assessment in the operating room, (3) design and synthesis of fluorescently labeled small molecule therapeutics for personalized therapy prediction in cancer, and (4) development of improved cyclic immunofluorescence methods to permit up to 50 color staining on a single tissue ...
Lab Tests Online - UK is a website written by practising laboratory doctors and scientists to help you understand the many clinical laboratory tests that are used in diagnosis, monitoring and treatment of disease ...
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet ...
The past couple months, the media has shined light on how dangerous COVID-19 can be, but it seems they have failed to mention the latest developments and simply preyed on the fear of Americans.. Looking back to March 3, WHO said the coronavirus death rate was 3.4%, around 34 times more lethal than that of the seasonal flu. With that given estimate, it was reasonable to close down stores, restaurants and take precautions necessary.. But as more data comes to light with the novel virus, it could be that the projected 3.4% death rate is greatly exaggerated.. A study conducted in early April at Santa Clara county concluded that a fatality rate for the county was around 0.1-0.2%, extremely similar to the seasonal flu. The fatality rate was far lower because of unconfirmed and asymptomatic cases.. However, it is important to not judge a virus fatality rate on just one study. That leads me to an antibody study conducted in New York.. New York is reporting 15,500 COVID-19 deaths, and if 2.7 million ...
HRP Conjugated anti-Rabbit IgG SABC Kit, with the specific secondary antibody and all the reagents needed for performing IHC-P;IHC-F;ICC
Filling a gap in the literature, this atlas stands as a unique authoritative source to thoroughly review the many immunofluorescence patterns seen in immunoderm
iPSc-EGFRvIII. CLTH/iPSc-EGFRvIII cell line is induced pluripotent stem cell line that stably expresses high level of EGF mutant receptor vIII and puromycin-resistant gen introduced by virus transduction. EGFRvIII is under EF1 promoter control, allowing high levels of protein expression. Immunofluorescent studies indicate expression of transcription factors (Sox2, Oct4, Nanog) as well as surface proteins (Tra1-81 and Tra1-60) characteristic for iPS cells.. ...
Find and order accessory reagents and products like Rabbit TrueBlot® Anti-Rabbit IgG HRP on www.antibodies-online.com. Order product ABIN1589973.
I: Demonstration of antibrain globulins by fluorescent antibody techniques. Arch Gen Psychiat 16: 1-9.. ...
This can be done with direct fluorescent antibody (DFA) techniques. It can also be achieved through indirect immunofluorescence ... Another form of microscopy is fluorescent microscopy done by staining with auramine. Other staining techniques include acid- ... vaccine has produced an antibody response in a large group of cows and also antibody response in calves fed rCP15/60-immune ... Antibodies in the serum of humans and animals infected with Cryptosporidium parvum react with several antigens, one of which is ...
Also, differential staining methods and fluorescent antibody techniques were introduced for in-situ observation of ... Various new techniques, ranging from the determination of growth and activity of microorganisms in the environments to their ... The new methods and techniques were proved to be successful for the analysis of microbial community in various fields, soil and ...
... by fluorescent-antibody technique". Journal of Invertebrate Pathology. 11 (2): 251-259. doi:10.1016/0022-2011(68)90158-4. ...
Later, Osborn and Weber pioneered fluorescent antibody staining of cellular substructures, a major technique called indirect ... then used fluorescently-tagged secondary antibodies (antibodies to the first set of antibodies) to light up the locations of ... and unreactive to actin antibodies. They developed new antibodies against proteins of the microtubules, intermediate filaments ... Many of their antibodies have been licensed to companies for commercial development. Klaus and Osborn used their method to ...
Burgdorfer, W. Evaluation of the fluorescent antibody technique for the detection of Rocky Mountain spotted fever rickettsiae ... A technique for detection of rickettsiae in ticks, Amer. J. Trop. Med. 19:1010-1014, 1970. Burgdorfer, W., Barbour, A. G., ...
... fluorescent antibody and cell culture isolation techniques for detection of antigen". Journal of Fish Diseases. 7: 57-64. doi: ...
This distribution of this antibody can then be visualized by a technique such as fluorescent labeling. Immunohistochemistry has ... In this technique, DNA that encodes a reporter gene is randomly inserted into the genome. Depending on the gene promoters ... Techniques that require fixation of tissue can only generate a single temporal time point per individual organism. However, ... For example, the reporter gene green fluorescent protein can be visualized by stimulating it with blue light and then using a ...
... a district in the Oromia Region of Ethiopia The Indirect Fluorescent Antibody Technique, a diagnostic process employing ...
To reduce the four-day waiting time needed for diagnosis, a method using the indirect fluorescent antibody technique (IFAT) was ...
Immunohistochemistry is a technique that uses antibodies with fluorescent staining tags that target a specific antigen present ... He created an immunoflorescent technique for labelling the antibodies. This technique continues to be widely used in ... Albert Coons used for the first time a revolutionary technique that uses the principle of antibodies binding specifically to ... These examples use FISH (Fluorescent in situ hybridization). With this technique we can understand the physiological processes ...
Benador Associates ^ "Detection of air-borne Pasteurella tularensis using the fluorescent antibody technique" by R. F. Jaeger, ...
... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... fluorescent antibody technique, direct MeSH E05.478.588.375.310 - fluorescent antibody technique, indirect MeSH E05.478.588.375 ... fluorescent antibody technique MeSH E05.478.588.375.050 - antibody-coated bacteria test, urinary MeSH E05.478.588.375.300 - ... 512.240.310 - fluorescent antibody technique, indirect MeSH E05.200.750.551.790 - periodic acid-schiff reaction MeSH E05.200. ...
She is also well known for her work in the development of the fluorescent antibody technique-a diagnostic technique used to ... "Isothiocyanate Compounds as Fluorescent Labeling Agents for Immune Serum". The American Journal of Pathology. 34 (6): 1081-1097 ...
... fluorescent antibody technique, direct MeSH E01.450.495.225.230 - fluorescent antibody technique, indirect MeSH E01.450.495.225 ... fluorescent antibody technique MeSH E01.450.495.225.050 - antibody-coated bacteria test, urinary MeSH E01.450.495.225.225 - ... fluorescent treponemal antibody absorption test MeSH E01.450.495.735.850.800 - treponema immobilization test MeSH E01.450. ... enzyme multiplied immunoassay technique MeSH E01.450.495.410.380 - immunosorbent techniques MeSH E01.450.495.410.380.200 - ...
"Laboratory techniques in rabies: the fluorescent antibody test." Monograph series. World Health Organization 23 (1973): 73. ... Fluorescent Antibody Test ବା FAT) ଏହାର ସ୍ୱର୍ଣ୍ଣିମ ପରୀକ୍ଷା ଯାହା ଡବ୍ଲ୍ୟୁ.ଏଚ୍.ଓ.ଦ୍ୱାରା ସ୍ଥିରିକୃତ ହୋଇଛି ।[୨୭] ମୃତ୍ୟୁ ପରେ ପଚି ... "Rabies antibodies in sera of wild birds" (PDF). Journal of Wildlife Diseases. 12 (3): 392-5. PMID 16498885 ...
... indirect fluorescent antibody testing, ELISA, PCR, and DNA probe technology techniques can be used to confirm diagnosis. The ...
... be cultured on specific growth mediums such as brain-heart infusion agar and techniques such as indirect fluorescent antibody ...
... an antigen can also be conjugated to the antibody with a fluorescent probe in a technique called fluorescent antigen technique ... This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets ... recognizes the primary antibody and binds to it. Multiple secondary antibodies can bind a single primary antibody. This ... Since the number of fluorescent molecules that can be bound to the primary antibody is limited, direct immunofluorescence is ...
Adam Reich, Katarzyna Marcinow, and Rafal Bialynicki-Birula Direct+Fluorescent+Antibody+Technique at the US National Library of ... A direct fluorescent antibody (DFA or dFA), also known as "direct immunofluorescence", is an antibody that has been tagged in a ... Direct fluorescent antibody can also be used to detect parasitic infections, as was pioneered by Sadun, et al. (1960). ... where the primary antibody binds the target antigen, with a secondary antibody directed against the primary, and a tag attached ...
... coli DNA extraction method plus PCR techniques. Newer technologies using fluorescent and antibody detection are also under ... Tzipori S, Sheoran A, Akiyoshi D, Donohue-Rolfe A, Trachtman H (October 2004). "Antibody therapy in the management of shiga ... such as the use of anti-induction strategies to prevent toxin production and the use of anti-Shiga toxin antibodies, have also ...
6: The fluorescent antibody test". In Kaplan MM, Koprowski H (eds.). Laboratory techniques in rabies. Monograph series. 23 (3rd ... The reference method for diagnosing rabies is the fluorescent antibody test (FAT), an immunohistochemistry procedure, which is ... to bind to and allow the visualisation of rabies antigen using fluorescent microscopy techniques. Microscopic analysis of ... Some light microscopy techniques may also be used to diagnose rabies at a tenth of the cost of traditional fluorescence ...
These cells are STED fluorescent dyes bound to antibodies through amide bonds. The first use of this technique coupled MR-121SE ... a red dye, with a secondary anti-mouse antibody. Since that first application, this technique has been applied to a much wider ... However, only fluorescent proteins provide the ability to visualize any organelle or protein in a living cell. This method was ... Using two fluorescent dyes and beam pairs, colocalized imaging of synaptic and mitochondrial protein clusters is possible with ...
Antiechinococcus antibodies can be detected with serodiagnostic tests - indirect fluorescent antibody, complement fixation, ... Cysts are detected with ultrasound, X-ray computed tomography, or other imaging techniques. ...
This technique is being used to characterize the immune glycome. Table 1:Advantages and disadvantages of mass spectrometry in ... This method uses either naturally occurring lectins or artificial monoclonal antibodies, where both are immobilized on a ... January 2009). "Comparison of fluorescent labels for oligosaccharides and introduction of a new postlabeling purification ... It is a non-scanning technique, wherein each transition is detected individually and the detection of multiple transitions ...
They are designed for labeling biomolecules, cells, tissues or beads in advanced fluorescent detection techniques. FluoProbes ... while FluoProbes labeled antibodies and cellular probes (i.e. Phalloidin) suit direct use in immunoassays or cell assays. ... Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes. "FluoProbes Dyes" (PDF). Interchim. 2010. ... The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. ...
... such as fluorescent staining, multi-angle light scatter and monoclonal antibody tagging. Most analyzers directly measure the ... In light scattering techniques, light from a laser or a tungsten-halogen lamp is directed at the stream of cells to collect ... These techniques work on the same principle of measuring the interruption in current as cells pass through an aperture, but ... Often, these antibodies are only active at room temperature (in which case they are called cold agglutinins), and the ...
... or by fluorescent antibodies. The technology may be used to determine whether a potential drug is disease modifying. For ... in the amounts of proteins synthesized by cells are measured using a variety of techniques such as the green fluorescent ... The wide use of the green fluorescent protein, a natural fluorescent protein molecule from jellyfish, then accelerated the ... In addition to fluorescent labeling, various label free assays have been used in high content screening. High-content screening ...
... fluorescent protein based methods, and mass-spectroscopy based methods. The antibody based methods use designed antibodies to ... Researchers are trying to develop a technique that can fulfil what current techniques are lacking: high throughput, higher ... Imaging: Antibodies can be bound to fluorescent molecules such as quantum dots or tagged with organic fluorophores for ... Antibody-DNA quantification: another antibody-based method converts protein levels to DNA levels. The conversion to DNA makes ...
... plus PCR techniques. Newer technologies using fluorescent and antibody detection are also under development. ... and the use of anti-Shiga toxin antibodies,[20] have also been proposed. ... "Antibody therapy in the management of shiga toxin-induced hemolytic uremic syndrome" (PDF). Clinical Microbiology Reviews. 17 ...
... (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only ... or imaged simultaneously with a fluorescent antibody assay. The technology has potential applications in cancer diagnosis,[14] ... A variety of other techniques uses mixtures of differently colored probes. A range of colors in mixtures of fluorescent dyes ... FISH is a very general technique. The differences between the various FISH techniques are usually due to variations in the ...
Käss, W. Tracing Technique in Geohydrology. Rotterdam: Balkema.. *^ The Story Behind Dyeing the River Green. Greenchicagoriver. ... Fluorescein-labelled probes can be imaged using FISH, or targeted by antibodies using immunohistochemistry. The latter is a ... In plant science, fluorescein, and other fluorescent dyes, have been used to monitor and study plant vasculature, particularly ... Yet another technique termed molecular beacons makes use of synthetic fluorescein-labeled oligonucleotides. ...
Quantitative IgE antibody assays in allergic diseases»։ Journal of Allergy and Clinical Immunology 105 (6): 1077-1084։ June ... fluorescent enzyme immunoassay FEIA)[84]: Այլ թեսթավորումներ[խմբագրել , խմբագրել կոդը]. Հսկիչ թեսթեր. երբ ենթադրյալ ալերգենը ... Revue Scientifique et Technique. 19 (1): 240-58. doi:10.20506/rst.19.1.1218. PMID 11189719. ... Specificity of IgE antibodies to sequential epitopes of hen's egg ovomucoid as a marker for persistence of egg allergy»։ ...
SERS-based immunoassay is a novel technique for detection of low abundance biomarkers. Using antibodies and gold particles this ... Each spectrum was specific, which is advantageous over fluorescence detection; some fluorescent markers overlap and interfere ... Surface-enhanced Raman spectroscopy or surface-enhanced Raman scattering (SERS) is a surface-sensitive technique that enhances ... Research in 2015 on a more powerful extension of the SERS technique called SLIPSERS (Slippery Liquid-Infused Porous SERS)[11] ...
These proteins can be stained with fluorescent dye labeled antibodies and detected using flow cytometry. The limit of detection ... Techniques for measuring minimal residual disease in leukaemia[edit]. DNA-based tests[edit]. These are based on detecting a ... Some new techniques use Next-Generation Sequencing to detect MRD. RNA-based tests[edit]. These are based on detecting a ... 2 Techniques for measuring minimal residual disease in leukaemia *2.1 DNA-based tests ...
This technique also uses an antibody to the protein of interest, along with classical electron microscopy techniques. The ... Fluorescent dyes are used to label cellular compartments for a similar purpose.[48] ... antibodies have no such constraints. An antibody's binding affinity to its target is extraordinarily high.[37] ... Antibodies are protein components of an adaptive immune system whose main function is to bind antigens, or foreign substances ...
Multiple displacement amplification (MDA) is a widely used technique, enabling amplifying femtograms of DNA from bacterium to ... by employing fluorescent in situ hybridization (FISH) and/or post-sequencing confirmation.[10] The bias of MDA against high %GC ... were introduced to simultaneously measure single-cell mRNA and protein expression through oligonucleotide-labeled antibodies ... a major improvement to this technique, called WGA-X, was introduced by taking advantage of a thermostable mutant of the phi29 ...
Also, transgenic techniques can create organisms that produce their own fluorescent chimeric molecules (such as a fusion of GFP ... Green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in ... An alternative technique is confocal theta microscopy. In this technique the cone of illuminating light and detected light are ... green fluorescent protein with the protein of interest).. Techniques used for horizontal scanning[edit]. Four types of confocal ...
For example, the use of antibodies made artificially fluorescent (fluorescently labeled antibodies) can be directed to bind to ... Complex serological techniques have been developed into what are known as Immunoassays. Immunoassays can use the basic antibody ... and can be made exquisitely specific when used in combination with antibody based techniques. ... Other techniques (such as X-rays, CAT scans, PET scans or NMR) are used to produce images of internal abnormalities resulting ...
How Lymphocytes Produce Antibody from Cells Alive!. *Antibody applications Fluorescent antibody image library, University of ... Rosetta Antibody is a novel antibody FV region structure prediction server, which incorporates sophisticated techniques to ... Antibody mimetic[edit]. Antibody mimetics are organic compounds that, like antibodies, can specifically bind antigens. They are ... Asymmetrical antibodies[edit]. Heterodimeric antibodies, which are also asymmetrical and antibodies, allow for greater ...
Hemolytic disease of the newborn:[9] This disease occurs when a newborn's red blood cells are being attacked by antibodies from ... poor tooth brushing technique, tobacco products, and exposure to iron salts and chlorhexidine can affect the colour of a tooth. ... the years of tooth development causes yellow-green discoloration of dentine visible through the enamel which is fluorescent ...
In addition to the ability to label and identify individual cells via fluorescent antibodies, cellular products such as ... The technique was expanded by Len Herzenberg, who was responsible for coining the term FACS.[27] Herzenberg won the Kyoto Prize ... Mass cytometry overcomes the fluorescent labeling limit by utilizing lanthanide isotopes attached to antibodies. This method ... Transgenic products in vivo, particularly the green fluorescent protein or related fluorescent proteins ...
The attachment of fluorescent group on one head of the molecule confers contrast properties. A new strategy to enhance the ... It was shown that Pep-1 could facilitate rapid cellular uptake of various peptides, proteins, and even full-length antibodies ... cells can be labeled in vitro with contrast agents and then they can be injected and monitored in vivo by using MRI techniques. ... Thereby, the authors of the study were able to deliver fluorescent antigen binding proteins into cells facilitating live-cell ...
This technique is typically coupled with reflection high energy electron diffraction (RHEED) and reflection high-energy loss ... The thin layer of tissue remains bound to the metal replica so it can be immunogold labeled with antibodies to the structures ... may be viewed by projecting the magnified electron image onto a fluorescent viewing screen coated with a phosphor or ... The technique required varies depending on the specimen and the analysis required: *Chemical fixation - for biological ...
Development of techniques that increase skin permeability has led to more drugs that can be applied via transdermal patches and ... Skin with high permeability allowed quantum dots with an antibody attached to the surface for active targeting to successfully ... Nanorods are used in experiments because of their unique fluorescent properties but have shown mediocre penetration. ... Nanoparticles along with multi-modal imaging techniques have been used as a way to diagnose cancer non-invasively. ...
A pancreatic islet that uses fluorescent antibodies to show the location of different cell types in the pancreatic islet. ... Spaull, Susan; Bruce-Gardyne, Lucinda (2003). Leiths Techniques Bible (1 ed.). Bloomsbury. p. 451. ISBN 0747560463. .. ... Antibodies against glucagon, secreted by alpha cells, show their peripheral position. Antibodies against insulin, secreted by ... "The Human Pancreas Proteome Defined by Transcriptomics and Antibody-Based Profiling". PLOS ONE. 9 (12): e115421. Bibcode ...
Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. ELISA ... fluorescent and DIG-labeled tracers for competitive immunoassays, i.e. to limit detect digoxin, a drug used to cure cardiac ... Journal of Biomolecular Techniques. 20 (2): 96-100. PMC 2685603. PMID 19503620.. ... The antibodies are labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection. ...
Hemolytic disease of the newborn:[9] This disease occurs when a newborn's red blood cells are being attacked by antibodies from ... Greenwall, Linda (2017-04-11), "Tooth Sensitivity Associated with Tooth Whitening", Tooth Whitening Techniques, CRC Press, pp. ... the years of tooth development causes yellow-green discoloration of dentine visible through the enamel which is fluorescent ... poor tooth brushing technique, tobacco products, and exposure to iron salts and chlorhexidine can darken the colour of a tooth. ...
Natural fluorescence (Many optical dosimetry techniques, such as fluorescence spectroscopy, depend on fluorescence.)[14] ... Strategies include directly attaching photosensitisers to biologically active molecules such as antibodies.[5] ... including oxygen transport and photosynthesis and have applications in fields ranging from fluorescent imaging to medicine. ...
The technique is recommended for couples who will not accept a child of the undesired gender.[27][28] ... Additional applications and methods were added, including antibodies, from 1987 through 1997.[35] At the time of the patent ... semen is labeled with a fluorescent dye called Hoechst 33342 which binds to the DNA of each spermatozoon. As the X chromosome ... The IVF/PGD technique is favored over the Ericsson method because of the stricter control of the offspring gender in the ...
Antibodies specific for a desired antigen can be conjugated with an isotopic (radio) or fluorescent label or with a color- ... and antigen has made the antibody an excellent tool for the detection of substances by a variety of diagnostic techniques. ... The humoral (antibody) response is defined as the interaction between antibodies and antigens.[16] Antibodies are specific ... Passively acquired maternal antibodies can suppress the antibody response to active immunization. Similarly the response of T- ...
In the development of the technique, five model cofactors with different topology constructs were used: TOP1-4 and INT with an ... CRISPR-Display can target the sgRNA with a particular epitope sequence to various loci, and fluorescently tagged antibodies can ... transient reporter gene expression of luciferase and fluorescent protein was measured. Two variations of such a transcription ... One example of artificial ncRNA functionalization is incorporating RNA domains recognized by specific antibodies to the sgRNA. ...
Mutagenesis (molecular biology technique). References[edit]. *^ "Terms and Acronyms". U.S. Environmental Protection Agency ... Human cells in which some proteins are fused with green fluorescent protein to allow them to be visualised ... Roque AC, Lowe CR, Taipa MA (2004). "Antibodies and genetically engineered related molecules: production and purification". ... The technique of gene targeting uses homologous recombination to make desired changes to a specific endogenous gene. This tends ...
This technique is useful as it reveals specific areas of gene expression in a tissue as well as throughout an entire embryo ... Labeling with a fluorescent dye or radioactive tag allows for the visualization of the probe and their location within the ... In contrast to in situ hybridization however, immunofluorescence uses a fluorophore attached to an antibody with biomolecule ... The use of such techniques vary with the stage of development and overall research goals, but include such methods as cell ...
Through this combined technique, mRNA is converted to cDNA, which is further quantified using qPCR. This technique lowers the ... However, antibodies don't appear until many weeks after infection, maternal antibodies mask the infection of a newborn, and ... The first method consists of using fluorescent dyes that are retained nonspecifically in between the double strands. The second ... An interesting technique combination is real-time PCR and reverse transcription. This sophisticated technique, called RT-qPCR, ...
... the virus particles separate the fluorescent dyes used for signalling to prevent the formation of non-fluorescent dimers that ... Antibodies can continue to be an effective defence mechanism even after viruses have managed to gain entry to the host cell. A ... The powerful techniques developed by life sciences are becoming the basis of engineering approaches towards nanomaterials, ... Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21). Proceedings of the National Academy ...
His invention became a central technique in biochemistry and molecular biology, described by The New York Times as "highly ... Why not divert a fraction of those antibodies to the influenza strain you just picked up? A chemical linker synthesized with an ... belief in astrology and an encounter with an extraterrestrial in the form of a fluorescent raccoon. ... Accreditation of the PCR technique[edit]. A concept similar to that of PCR had been described before Mullis' work. Nobel ...
The duo is also referred to as a fluorescently labeled antibody. ... Immunofluorescence refers to the combination of an antibody and ... fluorescent antibody technique A method for detecting the location of a specific antigen in a cell by staining a section of the ... fluorescent antibody technique A Dictionary of Plant Sciences © A Dictionary of Plant Sciences 1998, originally published by ... www.encyclopedia.com/science/dictionaries-thesauruses-pictures-and-press-releases/fluorescent-antibody-technique ...
Fluorescent antibody technique definition at Dictionary.com, a free online dictionary with pronunciation, synonyms and ... Either of two techniques used to test for antigen with a fluorescent antibody: direct, in which immunoglobulin conjugated with ... after which the antigen-antibody complex may be labeled with a fluorescent antibody. ... a fluorescent dye is added to tissue and combines with a specific antigen; or indirect, in which unlabeled immunoglobulin is ...
... techniques for locating antigens in a prepared tissue sample by using antibody,antibodies with fluorescent labels which ... ... fluorescent antibody techniques (thing). See all of fluorescent antibody techniques, no other writeups in this node. ... These are techniques for locating antigens in a prepared tissue sample by using antibodies with fluorescent labels which will ...
Use of Fluorescent-Antibody Staining of Cytocentrifuge-Prepared Smears in Combination with Cell Culture for Direct Detection of ... Indirect Fluorescent-Antibody Test, and Virus Isolation D. G. Bausch, P. E. Rollin, A. H. Demby, M. Coulibaly, J. Kanu, A. S. ... Use of Monoclonal Antibodies To Identify Serotypes of Enterovirus Isolates Alma S. Rigonan, Linda Mann, Tasnee Chonmaitree ... Evaluation of Indirect Fluorescence Antibody Assay for Detection of Bartonella clarridgeiae and Seroprevalence of B. ...
... Summary. Summary: A form of fluorescent antibody technique commonly used to detect ... viral antibodies*biological markers*fluorescent antibody technique*antineutrophil cytoplasmic antibodies*monoclonal antibodies* ... fluorescent antibody technique , indirect fluorescent antibody technique ... The exception is malaria antibody detection that is performed by the indirect fluorescent antibody technique using commercially ...
Chemical compound and disease context of Fluorescent Antibody Technique. *Biological context of Fluorescent Antibody Technique ... Gene context of Fluorescent Antibody Technique. *Analytical, diagnostic and therapeutic context of Fluorescent Antibody ... Gene context of Fluorescent Antibody Technique. *Immunofluorescence localization studies using affinity-purified antibody ... Associations of Fluorescent Antibody Technique with chemical compounds. *Both acetone and formaldehyde fixation were used for ...
... Series title. Journal of the Fisheries Research ... Myxosoma cerebralis: fluorescent antibody techniques for antigen recognition. Journal of the Fisheries Research Board of Canada ...
... relationships between the malaria parasites and piroplasms of mice as determined by the fluorescent-antibody technique.. ... relationships between the malaria parasites and piroplasms of mice as determined by the fluorescent-antibody technique.. ...
ENUMERATION OF CELL-INFECTING PARTICLES OF NEWCASTLE DISEASE VIRUS BY THE FLUORESCENT ANTIBODY TECHNIQUE. E. Frederick Wheelock ... ENUMERATION OF CELL-INFECTING PARTICLES OF NEWCASTLE DISEASE VIRUS BY THE FLUORESCENT ANTIBODY TECHNIQUE ... on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. ... Infective virus assayed by the fluorescent cell-counting procedure is expressed in terms of cell-infecting units (CIU). ...
Report of Six Cases and Study of the Renal Lesion by the Fluorescent Antibody Technique and Electron Microscopy DONALD A. ... Report of Six Cases and Study of the Renal Lesion by the Fluorescent Antibody Technique and Electron Microscopy. Ann Intern Med ...
Rapid Detection of Small Numbers of Airborne Bacteria by a Membrane Filter Fluorescent-Antibody Technique. Rolf Jost, Hans Fey ... Bacteria from stirred settling aerosols were recovered and identified by a rapid fluorescent-antibody technique previously ... Rapid Detection of Small Numbers of Airborne Bacteria by a Membrane Filter Fluorescent-Antibody Technique ... Rapid Detection of Small Numbers of Airborne Bacteria by a Membrane Filter Fluorescent-Antibody Technique ...
The fluorescent antibody technique in the diagnosis of a number of poultry diseases: manufacture of conjugates and their use [ ... The fluorescent antibody technique in the diagnosis of a number of poultry diseases: manufacture of conjugates and their use [ ... The fluorescent antibody technique in the diagnosis of a number of poultry diseases: manufacture of conjugates and their use [ ... The fluorescent antibody technique in the diagnosis of a number of poultry diseases: manufacture of conjugates and their use [ ...
"Fluorescent Antibody Technique" by people in this website by year, and whether "Fluorescent Antibody Technique" was a major or ... Fluorescent Antibody Technique*Fluorescent Antibody Technique. *Antibody Technique, Fluorescent. *Antibody Techniques, ... by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of ... "Fluorescent Antibody Technique" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ...
Describe the benefits of immunofluorescent antibody assays in comparison to nonfluorescent assays Compare direct and indirect ... Direct Fluorescent Antibody Techniques. Direct fluorescent antibody (DFA) tests use a fluorescently labeled mAb to bind and ... Indirect Fluorescent Antibody Techniques. Indirect fluorescent antibody (IFA) tests (Figure 20.29) are used to look for ... In a direct fluorescent antibody test, what does the fluorescent antibody bind to? ...
wp-content/uploads/2016/03/[email protected] 0 0 syn-admin /wp-content/uploads/2016/03/[email protected] syn-admin2002-02-01 20:22:552016-02-08 19:37:00SSeCKS immunolabeling in rat primary sensory neurons. ...
This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.. ...
Indirect Fluorescent Antibody Techniques. Indirect fluorescent antibody (IFA) tests ([link]) are used to look for antibodies in ... Direct Fluorescent Antibody Techniques. Direct fluorescent antibody (DFA) tests use a fluorescently labeled mAb to bind and ... Fluorescent Antibody Techniques. Learning Objectives. *Describe the benefits of immunofluorescent antibody assays in comparison ... In a direct fluorescent antibody test, what does the fluorescent antibody bind to? ...
Fluorescent Antibody Technique. Hs68 fibroblasts were seeded at a density of 105 cells/dish and treated with ADM for 14 days in ... The fluorescent image was photographed under fluorescent microscopy paired with CCD system. (B) The fluorescent intensity of ... Thereafter, the primary antibodies of anti-collagen I IgG produced in rabbit (1:500 in PBS, ab34710, Abcam, Cambridge, United ... After washing with PBS twice, the secondary antibody of H&L Dylight 594 anti-mouse IgG produced in goat (1:50 in PBS, ab96881 ...
... as a counterstain in fluorescent antibody staining techniques for the detection of antibodies to haemoprotozoa. / Jones, T. W. ... as a counterstain in fluorescent antibody staining techniques for the detection of antibodies to haemoprotozoa. In: Annals of ... as a counterstain in fluorescent antibody staining techniques for the detection of antibodies to haemoprotozoa. Annals of ... as a counterstain in fluorescent antibody staining techniques for the detection of antibodies to haemoprotozoa, Annals of ...
Evaluation of FA and conventional techniques for identifying group A beta hemolytic streptococci ...
... fluorescent antibody technique Remove constraint Subject: fluorescent antibody technique Start Over ... antibodies; blood serum; confidence interval; dogs; fluorescent antibody technique; humans; kidney diseases; myeloperoxidase; ... 1. Use of a Granulocyte Immunofluorescence Assay Designed for Humans for Detection of Antineutrophil Cytoplasmic Antibodies in ... BACKGROUND: Perinuclear antineutrophil cytoplasmic antibodies (pANCA) previously have been shown to be serum markers in dogs ...
Indirect Fluorescent Antibody Technique. The IFAT test is also an OIE approved screening method for BKD. The method detects ... labelled with a fluorescent dye is allowed to bind to those antibodies that have bound to the bacteria. A fluorescent antibody ... In some cases, if there is suspicion of BKD, an Indirect Fluorescent Antibody Technique (IFAT) test may be carried out on the ... Once bound a second antibody targeted against the first antibody (usually rabbit or sheep anti mouse antibodies) ...
New instruments for the laboratory diagnosis of hog cholera using the fluorescent antibody technique. *. W. Bommeli ... New instruments for the laboratory diagnosis of hog cholera using the fluorescent antibody technique}, author={W. Bommeli}, ...
1967). Quantitative fluorescent antibody technique in human and experimental schistosomiasis. Annales de la Société Belge de ... Quantitative fluorescent antibody technique in human and experimental schistosomiasis. In: Annales de la Société Belge de ... Quantitative fluorescent antibody technique in human and experimental schistosomiasis. Annales de la Société Belge de Médecine ... Quantitative fluorescent antibody technique in human and experimental schistosomiasis. PL Gigase, N Van Meirvenne, PG Janssens ...
Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein ... and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. ... Fluorescent Antibody Technique * Fluorescent Dyes* / chemistry * Genetic Techniques * Immunohistochemistry * Luminescent ... The fluorescent toolbox for assessing protein location and function Science. 2006 Apr 14;312(5771):217-24. doi: 10.1126/science ...
Fluorescent Antibody Technique* * Limbic System / anatomy & histology * Locus Coeruleus / anatomy & histology * Mammillary ... The projections of the ventral tegmental area and adjacent regions: a combined fluorescent retrograde tracer and ...
Fluorescent Antibody Technique. *Fluorescent Antibody Technique: methods. *Humans. *Liver Cirrhosis. *Liver Diseases ... Antibodies to SS-A/Ro-52kD and centromere in autoimmune liver disease: a clue to diagnosis and prognosis of primary biliary ... If confirmed in further studies with adequate follow-up, anti-SS-A/Ro-52kD antibodies might identify PBC patients with a more ... AIM: To investigate the frequency and significance of rheumatological antinuclear antibodies in the field of autoimmune ...
Fluorescent antibody technique in early syphilis. Arch Pathol. 1964; 77:220.PubMedGoogle Scholar ... antimicrobe antibodies) techniques may be important for microbe isolation. ... Fluorescent staining for mycobacteria in sarcoid and tuberculous granulomas. Am J Clin Pathol. 1969;51:584.PubMedGoogle Scholar ... IgG rheumatoid factor and antinuclear antibodies in rheumatoid vasculitis. Clin Exp Immunol. 1983; 52:333.Google Scholar ...
Other: fluorescent antibody technique. *(and 2 more...). Interventional. Phase 1. *University Health Network, Toronto ...
Fluorescent Antibody Technique (FAT). The most widely used test for postmortem rabies diagnosis is the fluorescent antibody ... D. J. Dean, M. K. Ableseth, and P. Atanasiu, "The fluorescent antibody test," in Laboratory Techniques in Rabies, F.-X. Meslin ... Figure 1: Fluorescent antibody technique (FAT) on human brain smear positive for rabies. ... P. Hostnik, "The modification of fluorescent antibody virus neutralization (FAVN) test for the detection of antibodies to ...
  • Resolution of details to the molecular level has been made possible during the 1990s with the advent of the technique of confocal laser microscopy. (encyclopedia.com)
  • The use of immunofluorescent compounds in combination with confocal microscopy has allowed the fluorescent probing of samples which do not need to be chemically preserved (or "fixed") prior to examination. (encyclopedia.com)
  • Fluorescent antibodies were also used, as well as advanced microscopy and molecular biology techniques. (news-medical.net)
  • For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. (gentaur.com)
  • This usually meant that enrichment culture techniques were required prior to immunofluorescence microscopy. (nanowerk.com)
  • The use of antibodies to antigen that are critical to disease processes in microorganisms allow immunofluorescence to act as a detection and screening tool in the monitoring of a variety of materials. (encyclopedia.com)
  • Jones, TW & Conrad, PA 1983, ' The use of Eriochrome black A (diamond black) as a counterstain in fluorescent antibody staining techniques for the detection of antibodies to haemoprotozoa ', Annals of Tropical Medicine and Parasitology , vol. 77, no. 5, pp. 535-536. (elsevier.com)
  • Recent advances in technology have led to the improvement or development of several diagnostic assays which include methods for rabies viral antigen and antibody detection and assays for viral nucleic acid detection and identification of specific biomarkers. (hindawi.com)
  • Fluorescent Western blotting can offer many advantages to an already robust protein detection technique. (jacksonimmuno.com)
  • The technique is highly sensitive, can be used quantitatively and allows for multiplex detection on the same blot without stripping and reprobing. (jacksonimmuno.com)
  • Figure 1: Indirect Fluorescent detection. (jacksonimmuno.com)
  • Fluorescent Western blotting offers a number advantages over chemiluminescent and colorimetric detection. (jacksonimmuno.com)
  • Fluorescent detection allows the blots to be used quantitatively because proteins on the blot are not perturbed by harsh stripping chemicals. (jacksonimmuno.com)
  • Jackson ImmunoResearch offers fluorescent dye-conjugated secondary antibodies that have minimal cross-reactivity with selected species, enabling detection of individual primary antibodies (and therefore target proteins) without signal overlap. (jacksonimmuno.com)
  • When combined with a wide dynamic range, optimized fluorescent Western blots can be used quantitatively, since both low and high abundance proteins can be detected on the same blot without saturating detection. (jacksonimmuno.com)
  • However to increase the sensitivity and specificity of antinuclear antibody detection further approaches were explored. (scribd.com)
  • This article provides an overview on advancement in antinuclear antibody detection methods, their future prospects, advantages, disadvantages and guidelines for use of these tests. (scribd.com)
  • Various detection methods are in use and there is continuous pouring of newer techniques to facilitate diagnosis and therapeutic monitoring in CTD patients. (scribd.com)
  • improvements in a method for the detection of antigen by means of fluorescent antibody. (abcam.com)
  • Current detection techniques such as ISO method 6579, fluorescent-antibody (FA), enzyme-linked immunosorbent assay (ELISA), or polymerase chain reaction (PCR) are time-consuming, cumbersome, and have limited sensitivity. (nanowerk.com)
  • A number of investigators have used the fluorescent-antibody (FA) technique for Salmonella detection. (nanowerk.com)
  • In some cases, if there is suspicion of BKD, an Indirect Fluorescent Antibody Technique (IFAT) test may be carried out on the fixed tissue. (thefishsite.com)
  • One of these, MAb VHS-10, reacted only with genotype IVa in indirect fluorescent antibody technique (IFAT) and ELISA. (int-res.com)
  • The prevalence of aspergilli and antibodies to different Aspergillus species were detected using cultural methods and serological technique like agar gel double diffusion (DD), counter-immuno-electrophoresis (CIEP) and enzyme-linked immunosorbent assay (ELISA). (jpgmonline.com)
  • Ten hybridoma clones secreting monoclonal antibodies (MAbs) against VHSV were established. (int-res.com)
  • Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques. (duke.edu)
  • Here, we have studied the capacity of anti-Env monoclonal antibodies (mAbs) against either the immunodominant region of gp41 (7B2 IgG1), the first constant region of gp120 (A32 IgG1), or the third variable loop (V3) of gp120 (CH22 IgG1) to modulate in vivo rectal mucosal transmission of a high-dose simian-human immunodeficiency virus (SHIV-BaL) in rhesus macaques. (duke.edu)
  • In the second option the target antigen molecule binds a socalled secondary antibody. (encyclopedia.com)
  • The secondary antibody then can itself be the target to which the fluorescently labeled antibody binds. (encyclopedia.com)
  • The serum is washed off and a secondary antibody added. (openstax.org)
  • A. Fluorescent dye-conjugated secondary antibody detects the primary antibody for the protein of interest. (jacksonimmuno.com)
  • Human fetal eyes, 9-20 weeks of gestation (wg), and adult human corneas with different diagnosis were treated for immunohistochemistry with specific antibodies against LM and alpha11 integrin chains. (diva-portal.org)
  • ELISA test showed 20% of cases to be having antibodies to A. fumigatus. (jpgmonline.com)
  • Allergic broncho-pulmonary aspergillosis can be detected in the early course of the disease in bronchial asthmatics using highly sensitive technique like ELISA. (jpgmonline.com)
  • Sera were tested for the presence of antibodies to extractable nuclear antigens (anti-ENA) by counterimmunoelectrophoresis, enzyme-linked and immunoblot (IB) assay, and for the presence of anti-centromere antibodies (ACA) by indirect immunofluorescence on HEp-2 cells and IB. (mendeley.com)
  • In contrast, antibodies that do not neutralize primary HIV-1 strains in the TZM-bl infection assay are readily induced by current vaccine candidates and have also been implicated as secondary correlates of decreased HIV-1 risk in the RV144 vaccine efficacy trial. (duke.edu)
  • Antigenic relationships between the malaria parasites and piroplasms of mice as determined by the fluorescent-antibody technique. (who.int)
  • Thus, the presence of different antibodies that are labeled with fluorescent compounds that fluoresce at the different wavelengths can produce an image of the location of two antigens in the same sample at the same time. (encyclopedia.com)
  • Here, we demonstrate a high-dynamic-range quantification of antibody binding to single antigens in a multiplexed suspension bead array format. (bioportfolio.com)
  • cytotoxic antibody any specific antibody directed against cellular antigens, which when bound to the antigen, activates the complement pathway or activates killer cells, resulting in cell lysis. (thefreedictionary.com)
  • It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. (ebookmall.com)
  • AIM: To investigate the frequency and significance of 'rheumatological' antinuclear antibodies in the field of autoimmune chronic liver disease, with special regard to PBC. (mendeley.com)
  • Antinuclear antibodies also occur in patients with rheumatoid arthritis, Sjögren's syndrome, and scleroderma. (thefreedictionary.com)
  • It has been more than 50 years since antinuclear antibodies were first discovered and found to be associated with connective tissue diseases. (scribd.com)
  • Connective tissue diseases (CTD) are a group of autoimmune disorders which are characterized by presence of antinuclear antibodies (ANA) in the blood of patients. (scribd.com)
  • The fluorescent antibody technique in the diagnosis of a number of poultry diseases: manufacture of conjugates and their use [virus diseases]. (fao.org)
  • For example, fluorescence-labeled antibodies against Streptococcus pyogenes ( group A strep ) can be used to obtain a diagnosis of strep throat from a throat swab. (openstax.org)
  • Antibodies to SS-A/Ro-52kD and centromere in autoimmune liver disease: a clue to diagnosis and prognosis of primary biliary cirrhosis. (mendeley.com)
  • The diagnosis of human African trypanosomiasis (T. gambiense) by the fluorescent antibody test. (geoscience.net)
  • For many decades immunofluorescent antinuclear antibody test has been the 'gold standard' in the diagnosis of these disorders. (scribd.com)
  • Not only are these antibodies involved in the disease pathogenesis, but they also constitute the basis for diagnosis and treatment of CTD. (scribd.com)
  • Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. (ebookmall.com)
  • The duo is also referred to as a fluorescently labeled antibody. (encyclopedia.com)
  • In a light microscopic application of the technique, sections of sample are exposed to the fluorescently labeled antibody. (encyclopedia.com)
  • In the first option, the fluorescently labeled antibody directly binds to the target antigen molecule. (encyclopedia.com)
  • Direct fluorescent antibody (DFA) tests use a fluorescently labeled mAb to bind and illuminate a target antigen. (openstax.org)
  • Ito T, Olesen NJ, Skall HF, Sano M, Kurita J, Nakajima K, Iida T (2010) Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa. (int-res.com)
  • The serum levels of IgA, IgG and IgM were determined by single radial immuno-diffusion technique in 104 male patients of acute viral hepatitis. (jpgmonline.com)
  • Thus, some antibodies that bind HIV-1 Env but fail to neutralize virus in traditional neutralization assays may limit the number of T/F viruses involved in transmission without leading to enhancement of viral infection. (duke.edu)
  • Viral hemagglutination inhibition tests for antibodies by the antibodies' ability to prevent viruses from agglutinating RBCs. (slideserve.com)
  • A procedure has been developed for the determination of the concentration of infective Newcastle disease virus (NDV) based on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. (rupress.org)
  • Phosphorylation of pp120 can also be triggered by attachment of cells to anti-integrin antibodies, and this requires the cytoplasmic domain of the integrin beta 1 subunit. (nih.gov)
  • Thus interaction of beta 1 integrins with extracellular ligands (fibronectin or antibodies) triggers phosphorylation of an intracellular 120-kDa protein, pp120, that may be involved in the responses of cells to attachment. (nih.gov)
  • Antibodies are synthesized by the plasma cells formed when antigen-specific groups ( clones ) of B lymphocytes respond to the presence of antigen. (thefreedictionary.com)
  • complete antibody antibody capable of agglutinating cells in physiologic saline solution. (thefreedictionary.com)
  • Immunofluorescence is a technique using antibodies to attach fluorescent dyes to cells. (sciencephoto.com)
  • Tools for evaluating -morphology:appearance can differentiate -immunophenotyping: use of antibodies to determine substances cells express. (brainscape.com)
  • In addition a fluorescent antibody technique was applied for the localization of α-chymotrypsin within the soybean cells. (wur.nl)
  • However, when we applied the fluorescent antibody technique to localize α-chymotrypsin within the cells from unheated sections, we used antibodies (γ-globulins) having a mol. (wur.nl)
  • A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. (labome.org)
  • Indirect fluorescent antibody (IFA) tests ( Figure 20.29 ) are used to look for antibodies in patient serum. (openstax.org)
  • Patient serum is spread over the smear and anti-treponemal antibodies, if present, are allowed to bind. (openstax.org)
  • On examination, the T. pallidum bacteria will only be visible if they have been bound by the antibodies from the patient's serum. (openstax.org)
  • BACKGROUND: Perinuclear antineutrophil cytoplasmic antibodies (pANCA) previously have been shown to be serum markers in dogs with chronic enteropathies, with dogs that have food‐responsive disease (FRD) having higher frequencies of seropositivity than dogs with steroid‐responsive disease (SRD). (usda.gov)
  • Through the use of a filter-paper technique to collect daily serum samples, it was possible to determine that the initial increase in FA response was on the 7th to 9th day of patency. (ajtmh.org)
  • However, when scrapings are negative, analysis of conjunctival and scleral specimens by histopathology (stainings and cultures), tissue homogenization (PCR, culture, or cell culture lines), and indirect immunofluorescence (antimicrobe antibodies) techniques may be important for microbe isolation. (springer.com)
  • Fluorescent Antibody Technique" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)
  • They can be used for high sensitivity, quantitative and in-gel Western blotting as well as microWestern, in-cell and on-cell Western arrays and other techniques that require the brightest dyes. (jacksonimmuno.com)
  • Then, other antigenic sites in the sample that might also bind the fluorescent antibody are "blocked" by the addition of a molecule that more globally binds to antigenic sites. (encyclopedia.com)
  • Rapid visualization of bacteria from a clinical sample such as a throat swab or sputum can be achieved through fluorescent antibody (FA) techniques that attach a fluorescent marker ( fluorogen ) to the constant region of an antibody, resulting in a reporter molecule that is quick to use, easy to see or measure, and able to bind to target markers with high specificity. (openstax.org)
  • The fluorescent dye absorbs light at its excitation wavelength, and then releases photons at its emission wavelength to return the molecule to its ground electronic state. (jacksonimmuno.com)
  • The light emitted by the fluorescent molecule (fluorophore) is then detected by a digital imager equipped with the correct filters. (jacksonimmuno.com)
  • an immunoglobulin molecule having a specific amino acid sequence that gives each antibody the ability to adhere to and interact only with the antigen that induced its synthesis. (thefreedictionary.com)
  • Small molecule fluorescent probes offer significant advantages over conventional antibody and fluorescent protein labeling techniques. (rsc.org)
  • 5 days after onset of symptoms, serologic techniques are used to detect IgM and/or IgG responses to the virus. (cdc.gov)
  • Like colorimetric and chemiluminescent Western blotting, fluorescent Western blotting uses the antigen-antibody complex to detect specific proteins immobilized on a blotting membrane after separation by electrophoresis. (jacksonimmuno.com)
  • The fluorescent antibody technique is used to detect disease-associated biomarkers (proteins). (konicaminolta.eu)
  • While the fixation regimens have been designed to avoid change of the sample's internal structure, especially the chemistry and three-dimensional structure of the site of the antigen to which the antibody will bind, the avoidance of any form of chemical modification is preferred. (encyclopedia.com)
  • These are technique s for locating antigen s in a prepared tissue sample by using antibodies with fluorescent labels which will bind to the antigen s of interest. (everything2.com)
  • As with the enzyme assays, FA methods may be direct, in which a labeled mAb binds an antigen, or indirect, in which secondary polyclonal antibodies bind patient antibodies that react to a prepared antigen. (openstax.org)
  • In a direct fluorescent antibody test, what does the fluorescent antibody bind to? (openstax.org)
  • Identification of Actinomyces israelii and Actinomyces naeslundii by fluorescent-antibody and agar-gel diffusion techniques. (medscape.com)
  • Technical report: xMAPr - High-dynamic-range (HDR) quantification of antigen-specific antibody binding. (bioportfolio.com)
  • This antigen-specific property of the antibody is the basis of the antigen-antibody reaction that is essential to an immune response . (thefreedictionary.com)
  • or indirect, in which unlabeled immunoglobulin is added to tissue and combines with a specific antigen, after which the antigen-antibody complex may be labeled with a fluorescent antibody. (dictionary.com)
  • The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (labome.org)
  • Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). (ucdenver.edu)
  • It is shown that, when particular conditions are met, the technique is a useful substitute for or adjunct to the isolation of virus, which requires more time and labour. (fao.org)
  • Bacteria from stirred settling aerosols were recovered and identified by a rapid fluorescent-antibody technique previously described by Danielsson. (asm.org)
  • Thus, the DFA technique is valuable for visualizing certain bacteria that are difficult to isolate or culture from patient samples. (openstax.org)
  • Figure 20.28 A green fluorescent mAb against L. pneumophila is used here to visualize and identify bacteria from a smear of a sample from the respiratory tract of a pneumonia patient. (openstax.org)
  • Newly developed staining techniques with fluorescent antibodies helped locate bacteria more accurately. (sciencentral.com)
  • Still, details of the trafficking of a protein from the site of its manufacture to the surface of a cell, for example, is possible, by the application of different antibodies. (encyclopedia.com)
  • A well-known example in biological and microbiological studies is the green fluorescent protein. (encyclopedia.com)
  • C. The excited fluorescent dye emits light at its emission wavelength which is detected via a digital imager, allowing visualization of the protein of interest. (jacksonimmuno.com)
  • Dye conjugated secondary antibodies directed against primaries raised in different species can be used to label each specific antigen (protein) with a dye with its own individual emission wavelength - spectral characteristics. (jacksonimmuno.com)
  • The nematodes used in the study were transgenic, with a fluorescent protein sequence inserted into their DNA to enable in vivo observation of protein expression. (news-medical.net)
  • The fluorescent compound that is attached to an antibody is able to absorb light of a certain wavelength, the particular wavelength being dependent on the molecular construction of the compound. (encyclopedia.com)
  • Here, we developed a molecular imprinting-based dynamic molding approach to fabricate antibody-conjugated signaling nanocavities capable of size recognition. (bioportfolio.com)
  • Based in molecular amplification techniques and host range specificity, several species of Cryptosporidium have been described. (clinicaladvisor.com)
  • Watch the animation on this page to review the procedures of the direct fluorescent antibody test. (openstax.org)
  • The IFA test for syphilis provides an important complement to the VDRL test discussed in Detecting Antigen-Antibody Complexes . (openstax.org)
  • IF-ANA: The standard ANA testing technique Before development of IF-ANA test.19]. (scribd.com)
  • The presence of cryptosporidium in stools can be determined by microscopic examination of stools using acid-fast staining and direct fluorescent antibody (DFA). (clinicaladvisor.com)
  • CONCLUSIONS: In the autoimmune liver disease setting, anti-SS-A/Ro-52kD and ACA have a high specificity for PBC and can thus be of diagnostic relevance in anti-mitochondrial antibodies negative cases. (mendeley.com)
  • One serologic testing method is the indirect fluorescent antibody (IFA) technique. (cdc.gov)
  • Meta-analysis: diagnostic accuracy of anti-cyclic citrullinated peptide antibody and rheumatoid factor for rheumatoid arthritis. (springer.com)
  • Fortunately, the immune response of antibody and complement can be transferred passively from one individual to another, as for example the transfer of maternal antibody across the placental barrier to the fetus, who has not yet developed a mature immune system. (thefreedictionary.com)
  • This activity is assisted by complement , which interacts with the antigen-antibody complex in such a way that the cell ruptures and there is dissolution ( lysis ) of the cell body. (thefreedictionary.com)
  • complement-fixing antibody antibody (primarily IgM and the IgG subclasses 1, 2, and 3) that activates complement when reacted with antigen. (thefreedictionary.com)
  • Paramushir virus was recently demonstrated to be serologically identical to Avalon virus by complement-fixation, neutralization, and fluorescent-antibody techniques (4). (cdc.gov)
  • Aim: The aim of this study to Assess the healing process of the immediate implant loading with two different provisional techniques (direct and indirect). (bioportfolio.com)
  • blocking antibody any antibody that by combining with an antigen blocks another immunologic reaction with the antigen. (thefreedictionary.com)
  • The antigen-antibody reaction begins as soon as substances interpreted as foreign invaders gain entrance into the body. (thefreedictionary.com)
  • Use of appropriate diagnostic techniques is key to identifying ranavirus infections and determining the effects of infection on host species. (springer.com)
  • We point out the limitations of certain diagnostic techniques, and identify needed areas of improvement. (springer.com)
  • With the availability of commercial serological tests that reliably can distinguish type-specific HSV antibodies, it is now possible to determine the type of maternal infection and, thus, further refine management of infants delivered to women who have active genital HSV lesions. (aappublications.org)
  • Get access to the best antibodies, discovery platforms, and know-how to advance your diagnostic and therapeutic programs. (abcam.com)
  • The research team, which also included Dr. Ralph A. Tripp from the Department of Infectious Diseases at the University of Georgia, fabricated a hetero-structured silicon/gold nanorod array by the glancing angle deposition (or GLAD) thin film method and functionalized it with anti- Salmonella antibodies and organic dye molecules. (nanowerk.com)
  • The preparation and testing of conjugates for use in the direct fluorescent antibody technique in infectious bursal disease, Newcastle disease, infectious laryngotracheitis and infectious bronchitis are described. (fao.org)
  • Far-red and infrared fluorescent dye conjugates such as Alexa Fluor ® 680 and 790 (Figure 2) offer greater sensitivity due to low fluorescence quenching, high extinction coefficients and low background autofluorescence. (jacksonimmuno.com)
  • Immunofluorescence refers to the combination of an antibody and a compound that will fluoresce when illuminated by light of a specific wavelength. (encyclopedia.com)
  • Investigation of the Antibacterial Activity of Ag-NPs Conjugated with a Specific Antibody against Staphylococcus aureus after Photoactivation. (bioportfolio.com)
  • All subjects studied (44) with the deformity who lived outside areas endemic for onchocerciasis were skin-snip negative and lacked specific antibody to onchocercal antigen. (ajtmh.org)
  • By making fluorescent materials bond chemically with the specific proteins and then detecting the fluorescence, the presence and behaviour of, for example, a sign of acute myocardial infarction can be shown up earlier. (konicaminolta.eu)
  • Reactions may be atypical in HIV positive patients and some degree loss of treponemal specific antibodies may occur over time in up to 10% of patents. (health.gov.au)
  • Properties If you buy Antibodies supplied by fabgen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This fabgen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. (gentaur.com)
  • Although FA procedures offer considerable time savings, a large number of the pathogen needs to be present in samples in order to observe detectable fluorescent signals. (nanowerk.com)
  • If confirmed in further studies with adequate follow-up, anti-SS-A/Ro-52kD antibodies might identify PBC patients with a more advanced and active disease. (mendeley.com)
  • Maintenance hosts are essential for the continued existence of the virus, usually living in symbiosis with the viruses, without actual disease, but they do develop antibodies. (educate-yourself.org)
  • The response of monkeys infected with Plasmodium knowlesi was followed using the indirect fluorescent-antibody technique. (ajtmh.org)