A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.
A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.
Visualization of a vascular system after intravenous injection of a fluorescein solution. The images may be photographed or televised. It is used especially in studying the retinal and uveal vasculature.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
Measurement of light given off by fluorescein in order to assess the integrity of various ocular barriers. The method is used to investigate the blood-aqueous barrier, blood-retinal barrier, aqueous flow measurements, corneal endothelial permeability, and tear flow dynamics.
The concave interior of the eye, consisting of the retina, the choroid, the sclera, the optic disk, and blood vessels, seen by means of the ophthalmoscope. (Cline et al., Dictionary of Visual Science, 4th ed)
Organic derivatives of thiocyanic acid which contain the general formula R-SCN.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The blood vessels which supply and drain the RETINA.
A pathological process consisting of the formation of new blood vessels in the CHOROID.
The thin, highly vascular membrane covering most of the posterior of the eye between the RETINA and SCLERA.
The fluid secreted by the lacrimal glands. This fluid moistens the CONJUNCTIVA and CORNEA.
A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.
Corneal and conjunctival dryness due to deficient tear production, predominantly in menopausal and post-menopausal women. Filamentary keratitis or erosion of the conjunctival and corneal epithelium may be caused by these disorders. Sensation of the presence of a foreign body in the eye and burning of the eyes may occur.
The transparent, semigelatinous substance that fills the cavity behind the CRYSTALLINE LENS of the EYE and in front of the RETINA. It is contained in a thin hyaloid membrane and forms about four fifths of the optic globe.
A tricarbocyanine dye that is used diagnostically in liver function tests and to determine blood volume and cardiac output.
The use of green light-producing LASERS to stop bleeding. The green light is selectively absorbed by HEMOGLOBIN, thus triggering BLOOD COAGULATION.
Clarity or sharpness of OCULAR VISION or the ability of the eye to see fine details. Visual acuity depends on the functions of RETINA, neuronal transmission, and the interpretative ability of the brain. Normal visual acuity is expressed as 20/20 indicating that one can see at 20 feet what should normally be seen at that distance. Visual acuity can also be influenced by brightness, color, and contrast.
A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.
Measurement of the intensity and quality of fluorescence.
A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
Disorders of the choroid including hereditary choroidal diseases, neoplasms, and other abnormalities of the vascular layer of the uvea.
Degenerative changes in the RETINA usually of older adults which results in a loss of vision in the center of the visual field (the MACULA LUTEA) because of damage to the retina. It occurs in dry and wet forms.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Examination of the interior of the eye with an ophthalmoscope.
An oval area in the retina, 3 to 5 mm in diameter, usually located temporal to the posterior pole of the eye and slightly below the level of the optic disk. It is characterized by the presence of a yellow pigment diffusely permeating the inner layers, contains the fovea centralis in its center, and provides the best phototropic visual acuity. It is devoid of retinal blood vessels, except in its periphery, and receives nourishment from the choriocapillaris of the choroid. (From Cline et al., Dictionary of Visual Science, 4th ed)
An analytical method for detecting and measuring FLUORESCENCE in compounds or targets such as cells, proteins, or nucleotides, or targets previously labeled with FLUORESCENCE AGENTS.
An imaging method using LASERS that is used for mapping subsurface structure. When a reflective site in the sample is at the same optical path length (coherence) as the reference mirror, the detector observes interference fringes.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Sterile solutions that are intended for instillation into the eye. It does not include solutions for cleaning eyeglasses or CONTACT LENS SOLUTIONS.
A specialized transport barrier, in the EYE, formed by the retinal pigment EPITHELIUM, and the ENDOTHELIUM of the BLOOD VESSELS of the RETINA. TIGHT JUNCTIONS joining adjacent cells keep the barrier between cells continuous.
A tetraiodofluorescein used as a red coloring in some foods (cherries, fish), as a disclosure of DENTAL PLAQUE, and as a stain of some cell types. It has structural similarity to THYROXINE.
Fluid accumulation in the outer layer of the MACULA LUTEA that results from intraocular or systemic insults. It may develop in a diffuse pattern where the macula appears thickened or it may acquire the characteristic petaloid appearance referred to as cystoid macular edema. Although macular edema may be associated with various underlying conditions, it is most commonly seen following intraocular surgery, venous occlusive disease, DIABETIC RETINOPATHY, and posterior segment inflammatory disease. (From Survey of Ophthalmology 2004; 49(5) 470-90)
Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.
A red fluorescein dye used as a histologic stain. It may be cytotoxic, mutagenic, and inhibit certain mitochondrial functions.
Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.
The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.
Central retinal artery and its branches. It arises from the ophthalmic artery, pierces the optic nerve and runs through its center, enters the eye through the porus opticus and branches to supply the retina.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Blockage of the RETINAL VEIN. Those at high risk for this condition include patients with HYPERTENSION; DIABETES MELLITUS; ATHEROSCLEROSIS; and other CARDIOVASCULAR DISEASES.
The space in the eye, filled with aqueous humor, bounded anteriorly by the cornea and a small portion of the sclera and posteriorly by a small portion of the ciliary body, the iris, and that part of the crystalline lens which presents through the pupil. (Cline et al., Dictionary of Visual Science, 4th ed, p109)
Diseases of the uvea.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
The photography of images produced on a fluorescent screen by X-rays.
Method of making images on a sensitized surface by exposure to light or other radiant energy.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Central retinal vein and its tributaries. It runs a short course within the optic nerve and then leaves and empties into the superior ophthalmic vein or cavernous sinus.
Sudden ISCHEMIA in the RETINA due to blocked blood flow through the CENTRAL RETINAL ARTERY or its branches leading to sudden complete or partial loss of vision, respectively, in the eye.
Bleeding from the vessels of the retina.
The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Organic compounds which contain mercury as an integral part of the molecule.
Formation of new blood vessels originating from the retinal veins and extending along the inner (vitreal) surface of the retina.
An area approximately 1.5 millimeters in diameter within the macula lutea where the retina thins out greatly because of the oblique shifting of all layers except the pigment epithelium layer. It includes the sloping walls of the fovea (clivus) and contains a few rods in its periphery. In its center (foveola) are the cones most adapted to yield high visual acuity, each cone being connected to only one ganglion cell. (Cline et al., Dictionary of Visual Science, 4th ed)
The white, opaque, fibrous, outer tunic of the eyeball, covering it entirely excepting the segment covered anteriorly by the cornea. It is essentially avascular but contains apertures for vessels, lymphatics, and nerves. It receives the tendons of insertion of the extraocular muscles and at the corneoscleral junction contains the canal of Schlemm. (From Cline et al., Dictionary of Visual Science, 4th ed)
The administration of substances into the VITREOUS BODY of the eye with a hypodermic syringe.
The clear, watery fluid which fills the anterior and posterior chambers of the eye. It has a refractive index lower than the crystalline lens, which it surrounds, and is involved in the metabolism of the cornea and the crystalline lens. (Cline et al., Dictionary of Visual Science, 4th ed, p319)
Separation of the inner layers of the retina (neural retina) from the pigment epithelium. Retinal detachment occurs more commonly in men than in women, in eyes with degenerative myopia, in aging and in aphakia. It may occur after an uncomplicated cataract extraction, but it is seen more often if vitreous humor has been lost during surgery. (Dorland, 27th ed; Newell, Ophthalmology: Principles and Concepts, 7th ed, p310-12).
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
The most anterior portion of the uveal layer, separating the anterior chamber from the posterior. It consists of two layers - the stroma and the pigmented epithelium. Color of the iris depends on the amount of melanin in the stroma on reflection from the pigmented epithelium.
Green dyes containing ammonium and aryl sulfonate moieties that facilitate the visualization of tissues, if given intravenously. They have mostly been used in the study of kidney physiology.
The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball.
The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The rate dynamics in chemical or physical systems.
Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
Therapy using oral or topical photosensitizing agents with subsequent exposure to light.
Swelling of the OPTIC DISK, usually in association with increased intracranial pressure, characterized by hyperemia, blurring of the disk margins, microhemorrhages, blind spot enlargement, and engorgement of retinal veins. Chronic papilledema may cause OPTIC ATROPHY and visual loss. (Miller et al., Clinical Neuro-Ophthalmology, 4th ed, p175)
Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.
The administration of substances into the eye with a hypodermic syringe.
Recording of electric potentials in the retina after stimulation by light.
Introduction of substances into the body using a needle and syringe.
The selectively permeable barrier, in the EYE, formed by the nonpigmented layer of the EPITHELIUM of the CILIARY BODY, and the ENDOTHELIUM of the BLOOD VESSELS of the IRIS. TIGHT JUNCTIONS joining adjacent cells keep the barrier between cells continuous.
A visual impairment characterized by the accumulation of fluid under the retina through a defect in the retinal pigment epithelium.
Inflammation of the choroid.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Devices for examining the interior of the eye, permitting the clear visualization of the structures of the eye at any depth. (UMDNS, 1999)
Drying and inflammation of the conjunctiva as a result of insufficient lacrimal secretion. When found in association with XEROSTOMIA and polyarthritis, it is called SJOGREN'S SYNDROME.
Inflammation of the retinal vasculature with various causes including infectious disease; LUPUS ERYTHEMATOSUS, SYSTEMIC; MULTIPLE SCLEROSIS; BEHCET SYNDROME; and CHORIORETINITIS.
The prototypical uricosuric agent. It inhibits the renal excretion of organic anions and reduces tubular reabsorption of urate. Probenecid has also been used to treat patients with renal impairment, and, because it reduces the renal tubular excretion of other drugs, has been used as an adjunct to antibacterial therapy.
Microscopy in which the image is formed by ultraviolet radiation and is displayed and recorded by means of photographic film.
Small breaks in the elastin-filled tissue of the retina.
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
Discharge of cerebrospinal fluid through the nose. Common etiologies include trauma, neoplasms, and prior surgery, although the condition may occur spontaneously. (Otolaryngol Head Neck Surg 1997 Apr;116(4):442-9)
The escape of diagnostic or therapeutic material from the vessel into which it is introduced into the surrounding tissue or body cavity.
The minute vessels that connect the arterioles and venules.
The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT.
Measurement of the various properties of light.
The sebaceous glands situated on the inner surface of the eyelids between the tarsal plates and CONJUNCTIVA.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
Elements of limited time intervals, contributing to particular results or situations.
Permanent dilation of preexisting blood vessels (CAPILLARIES; ARTERIOLES; VENULES) creating small focal red lesions, most commonly in the skin or mucous membranes. It is characterized by the prominence of skin blood vessels, such as vascular spiders.
Diseases affecting the eye.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
Colloid or hyaline bodies lying beneath the retinal pigment epithelium. They may occur either secondary to changes in the choroid that affect the pigment epithelium or as an autosomal dominant disorder of the retinal pigment epithelium.
The metal-free red phycobilin pigment in a conjugated chromoprotein of red algae. It functions as a light-absorbing substance together with chlorophylls.

The bystander effect in the HSVtk/ganciclovir system and its relationship to gap junctional communication. (1/2851)

The bystander effect (BSE) is an interesting and important property of the herpes thymidine kinase/ganciclovir (hTK/GCV) system of gene therapy for cancer. With the BSE, not only are the hTK expressing cells killed upon ganciclovir (GCV) exposure but also neighboring wild-type tumor cells. On testing a large number of tumor cell lines in vitro, a wide range of sensitivity to bystander killing was found. Since transfer of toxic GCV metabolites from hTK-modified to wild-type tumor cells via gap junctions (GJ) seemed to be a likely mechanism of the BSE, we tested GJ function in these various tumors with a dye transfer technique and pharmacological agents known to affect GJ communication. We confirmed that mixtures of tumor cell resistant to the BSE did not show dye transfer from cell to cell while bystander-sensitive tumor cells did. Dieldrin, a drug known to decrease GJ communication, diminished dye transfer and also inhibited the BSE. Forskolin, an upregulator of cAMP did increase GJ, but directly inhibited hTK and therefore its effect on BSE could not be determined. We conclude that these observations further support port the concept that functional GJ play an important role in the BSE and further suggest that pharmacological manipulation of GJ may influence the outcome of cancer therapy with hTK/GCV.  (+info)

Overexpression of the multidrug resistance-associated protein (MRP1) in human heavy metal-selected tumor cells. (2/2851)

Cellular and molecular mechanisms involved in the resistance to cytotoxic heavy metals remain largely to be characterized in mammalian cells. To this end, we have analyzed a metal-resistant variant of the human lung cancer GLC4 cell line that we have selected by a step-wise procedure in potassium antimony tartrate. Antimony-selected cells, termed GLC4/Sb30 cells, poorly accumulated antimony through an enhanced cellular efflux of metal, thus suggesting up-regulation of a membrane export system in these cells. Indeed, GLC4/Sb30 cells were found to display a functional overexpression of the multidrug resistance-associated protein MRP1, a drug export pump, as demonstrated by Western blotting, reverse transcriptase-polymerase chain reaction and calcein accumulation assays. Moreover, MK571, a potent inhibitor of MRP1 activity, was found to markedly down-modulate resistance of GLC4/Sb30 cells to antimony and to decrease cellular export of the metal. Taken together, our data support the conclusion that overexpression of functional MRP1 likely represents one major mechanism by which human cells can escape the cytotoxic effects of heavy metals.  (+info)

Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers. (3/2851)

A fluorimetric multi-parameter cell sensor at the single cell level is presented which makes it possible to observe the physiological behavior of different cell lines, different physiological parameters, and statistical data at the same time. Different cell types were immobilized at predefined positions with high accuracy using optical tweezers and adhesion promoting surface layers. The process is applicable to both adherent and non-adherent cells. Coating of the immobilization area with mussel adhesive protein was shown to be essential for the process. Intracellular proton and calcium concentrations in different cell classes were simultaneously imaged and the specific activation of T lymphocytes was demonstrated. This method should be especially useful for drug screening due to the small sample volume and high information density.  (+info)

Shrinkage-induced activation of Na+/H+ exchange in rat renal mesangial cells. (4/2851)

Using the pH-sensitive dye 2', 7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF), we examined the effect of hyperosmolar solutions, which presumably caused cell shrinkage, on intracellular pH (pHi) regulation in mesangial cells (single cells or populations) cultured from the rat kidney. The calibration of BCECF is identical in shrunken and unshrunken mesangial cells if the extracellular K+ concentration ([K+]) is adjusted to match the predicted intracellular [K+]. For pHi values between approximately 6.7 and approximately 7.4, the intrinsic buffering power in shrunken cells (600 mosmol/kgH2O) is threefold larger than in unshrunken cells (approximately 300 mosmol/kgH2O). In the nominal absence of CO2/HCO-3, exposing cell populations to a HEPES-buffered solution supplemented with approximately 300 mM mannitol (600 mosmol/kgH2O) causes steady-state pHi to increase by approximately 0.4. The pHi increase is due to activation of Na+/H+ exchange because, in single cells, it is blocked in the absence of external Na+ or in the presence of 50 microM ethylisopropylamiloride (EIPA). Preincubating cells in a Cl--free solution for at least 14 min inhibits the shrinkage-induced pHi increase by 80%. We calculated the pHi dependence of the Na+/H+ exchange rate in cell populations under normosmolar and hyperosmolar conditions by summing 1) the pHi dependence of the total acid-extrusion rate and 2) the pHi dependence of the EIPA-insensitive acid-loading rate. Shrinkage alkali shifts the pHi dependence of Na+/H+ exchange by approximately 0.7 pH units.  (+info)

Distribution of 5-chloromethylfluorescein diacetate staining during meiotic maturation and fertilization in vitro of mouse oocytes. (5/2851)

The aim of this confocal microscopy study was to determine whether the pattern of CellTracker Green 5-chloromethylfluorescein diacetate (CMFDA) staining changes during meiotic maturation and fertilization in vitro of mouse oocytes. At different times during meiotic maturation and fertilization, oocytes, zygotes and two-cell embryos were stained with CMFDA to demonstrate intracellular glutathione S-transferase activity. After washing in CMFDA-free medium, most oocytes, zygotes and embryos were stained with dihydroethidium (HE) to visualize DNA structures. Meiotic maturation and fertilization in vitro of mouse oocytes were associated with changes in the pattern of intracellular CMFDA staining. In particular, accumulations of CMFDA-positive membranes were observed around the nucleus of germinal vesicle (GV) oocytes, overlaying the sperm nucleus as well as overlaying the first mitotic spindle if this approached the plasma membrane. Staining of oocytes and zygotes with the probes 3,3'-dihexyloxacarbocyanine iodine [DiOC6(3)], which stains all the intracellular membranes, and rhodamine 123, which stains active mitochondria, demonstrated that the intracellular structures evidenced by CMFDA staining did not correspond to accumulations of mitochondria. Exposure of oocytes and zygotes to the microtubule-disrupting agent nocodazole or the actin-depolymerizing drug cytochalasin D revealed an autonomous microfilament-dependent transport and relocation of CMFDA-positive membranes during meiotic maturation and fertilization. Such a transport of CMFDA-positive membranes may be envisaged as a protective shield built to prevent damage to DNA from endogenous and exogenous mutagen metabolites.  (+info)

Partitioning of triphenylalkylphosphonium homologues in gel bead-immobilized liposomes: chromatographic measurement of their membrane partition coefficients. (6/2851)

Unilamellar liposomes of small or large size, SUVs and LUVs, respectively, were stably immobilized in the highly hydrophilic Sepharose 4B or Sephacryl S-1000 gel beads as a membrane stationary phase for immobilized liposome chromatography (ILC). Lipophilic cations of triphenylmethylphosphonium and tetraphenylphosphonium (TPP+) have been used as probes of the membrane potential of cells. Interaction of TPP+ and triphenylalkylphosphonium homologues with the immobilized liposomal membranes was shown by their elution profiles on both zonal and frontal ILC. Retardation of the lipophilic cations on the liposome gel bed was increased as the hydrophobicity of the cations increased, indicating the partitioning of lipophilic cations into the hydrocarbon region of the membranes. The cations did not retard on the Sepharose or Sephacryl gel bed without liposomes, confirming that the cations only interact with the immobilized liposomes. Effects of the solute concentration, flow rate, and gel-matrix substance on the ILC were studied. The stationary phase volume of the liposomal membranes was calculated from the volume of a phospholipid molecule and the amount of the immobilized phospholipid, which allowed us to determine the membrane partition coefficient (KLM) for the lipophilic cations distributed between the aqueous mobile and membrane stationary phases. The values of KLM were generally increased with the hydrophobicity of the solutes increased, and were higher for the SUVs than for the LUVs. The ILC method described here can be applied to measure membrane partition coefficients for other lipophilic solutes (e.g., drugs).  (+info)

Chemical transformations in individual ultrasmall biomimetic containers. (7/2851)

Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes.  (+info)

Kinetics of lactate and pyruvate transport in cultured rat myotubes. (8/2851)

Skeletal muscle transport of lactate and pyruvate was studied in primary cultures of rat myotubes, applying the pH-sensitive fluorescent indicator 2', 7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. The initial rate of decrease in intracellular pH (pHi) upon lactate or pyruvate incubation was used to determine total transport (carrier mediated and diffusion). Both lactate and pyruvate transport could be inhibited by a combination of 0.5 mM 4,4'-diisothiocyanostilbene-2, 2'-disulfonic acid, 5 mM mersalyl and 10 mM alpha-cyano-4-hydroxycinnamate. The kinetic parameters, Km and Vmax, for carrier-mediated transport of lactate were 9.9+/-1.1 mM and 0. 69+/-0.02 mmol l-1 s-1, respectively. For pyruvate, Km and Vmax were 4.4+/-1.3 mM and 0.30+/-0.05 mmol l-1 s-1, respectively. The diffusion component of the total transport was 0.0040+/-0.0005[S] (n=4) and 0.0048+/-0.0003[S] (n=4) for lactate and pyruvate, respectively. Furthermore, it was observed that the two monocarboxylate transporter isoforms present in mature skeletal muscles, MCT1 and MCT4 (formerly called MCT3 (M.C. Wilson, V.N. Jackson, C. Heddle, N.T. Price, H. Pilegaard, C. Juel, A. Bonen, I. Montgomery, O.F. Hutter, A.P. Halestrap, Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3, J. Biol. Chem. 273 (1998) 15920-15926)), were also expressed in primary culture of myotubes.  (+info)

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Synonyms for direct fluorescein-conjugated antibody in Free Thesaurus. Antonyms for direct fluorescein-conjugated antibody. 21 words related to antibody: active site, protein, autoantibody, precipitin, ABO antibodies, Rh antibody, antitoxin, agglutinin, Forssman antibody.... What are synonyms for direct fluorescein-conjugated antibody?
A vital dye used in biological research. One use is in determining the viability of pollen grains; grains which are alive take up the fluorescein diacet...
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at pH 7 makes it easy to prepare green-fluorescent thiol conjugates of biomolecules. 5- Iodoacetamidofluorescein has been one of the most extensively used visible wavelength- excitable, thiol-reactive dyes for modification of proteins, nucleic acids and other biopolymers in the literature. Following conjugation to thiols, fluorescein-5- iodoacetamide can be radioiodinated. Conjugates of M0638 have a high intrinsic detectability, particularly when used with instrumentation that incorporate the 488 nm spectral line of the argon-ion laser. Furthermore, its negative charge also makes capillary electrophoretic separation of labeled adducts possible. (MGT inc. Product information sheet www.markergen.com) ...
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Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye. CFSE is cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules,[1] notably, to intracellular lysine residues and other amine sources. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Also, due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. CFSE is commonly confused with carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), although they are not strictly the same molecule; CFDA-SE, due to its acetate groups, is highly cell permeable, while CFSE is much less so. As CFDA-SE, which is non-fluorescent, enters the cytoplasm of cells, intracellular esterases remove the acetate groups and convert the molecule to the fluorescent ester. CFSE was originally developed as a fluorescent dye that could be used to stably label lymphocytes and track their migration within ...
Large benthic foraminifera are unicellular calcifying reef organisms that can form symbiotic relationships with a range of different microalgae. However, the cellular functions, such as symbiosis and calcification, and other aspects of cellular physiology in large benthic foraminifera are not fully understood. Amphisorus kudakajimensis was used as a model to determine the detailed cellular characteristics of large benthic foraminifera. We used calcein acetoxymethyl ester (calcein AM) as a fluorescent indicator for live confocal imaging. We demonstrated that calcein AM is a useful fluorescent indicator to stain the fine network of reticulopodia and the cytoplasm in living A. kudakajimensis. We showed that at least two types of reticulopodia exist in A. kudakajimensis: the straight bundle of reticulopodia that spreads from the aperture and the fine reticulopodia along the surface of the aperture and chamber walls. The cytoplasm in outer chambers was highly branched and contained a few dinoflagellates. In
Anti-fluorescein antibodies are excellent model systems for studying the biochemical basis of molecular recognition because a prodigious amount of both physico-chemical and structural information is available for these antibodies. Furthermore, recombinant single-chain antibodies have been produced for several anti-fluorescein antibodies, and site-specific mutagenesis studies have defined the energetic contributions of a number of key active-site residues. In previous studies, we determined the three-dimensional structure of an antigen-binding fragment of a high-affinity anti-fluorescein antibody (4-4-20) in complex with fluorescein. These studies showed that fluorescein binds tightly in an aromatic slot and participates in a network of electrostatic interactions. In this report, we examine the role of electrostatic interactions in the 4-4-20 antigen-combining site by observing the effects of pH on the fluorescence of fluorescein and antigen-binding affinity. These studies showed that the salt ...
Animals. All mice were used at 6-8 weeks of age. C57BL/6 mice were purchased from the National Cancer Institute (Frederick, Maryland, USA). A breeding pair of lymphotoxin-α (LTα) knockout mice (on a mixed 129 × C57BL/6 background) were originally obtained from David Chaplin (Washington University, St. Louis, Missouri, USA) and have since been backcrossed for more than eight generations onto a C57BL/6 background. For some experiments, LTα knockout mice were splenectomized 2 weeks prior to immunization. Splenectomies were performed by staff of the Veterinarian Care Services at Yale University.. Antigens. L. major promastigotes of the WR309 substrain were maintained at 23°C in Schneiders Drosophila medium (GIBCO BRL; Invitrogen Corporation, Grand Island, New York, USA) supplemented with 20% FCS and 50 μg/ml gentamicin. For some experiments, L. major parasites were labeled with CFSE (5- and 6-carboxyfluorescein diacetate succinimidyl ester; Molecular Probes Inc., Eugene, Oregon, USA) using a ...
ANA M. MATA, ANN E. SCHOFIELD, JANE WOODBINE, ANTHONY G. LEE, J. MALCOLM EAST; Probing the nucleotide binding site of sarcoplasmic reticulum (Ca2+ -Mg2)-ATPase with anti-fluorescein antibodies. Biochem Soc Trans 1 December 1989; 17 (6): 1105-1106. doi: https://doi.org/10.1042/bst0171105. Download citation file:. ...
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The present investigation has demonstrated that ionizing radiation in the therapeutic dose range stimulates a transient cellular generation of ROS/RNS. Temporally coincident is a radiation-induced reversible depolarization of the mitochondrial ΔΨ and decrease in mitochondrial entrapped calcein fluorescence, both hallmarks of the mitochondrial permeability transition. The amount of ROS/RNS generated is relatively constant over the dose range tested, but the number of cells that respond increases with the dose. The radiation-induced ROS/RNS generation, ΔΨ depolarization, and calcein release are inhibited by CsA but not by the structural analogue, CsH. Overexpression of the Ca2+-binding protein calbindin 28K or treatment of cells with BAPTA/AM, an intracellular Ca2+ chelator, also effectively block radiation-induced ROS/RNS. The increased ROS/RNS generation observed with radiation is common to all of the cell types examined but for one important exception, the mitochondrial DNA-less ρo ...
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TRPV4 was originally identified as a plasma membrane channel activated by hypotonic cell swelling (Liedtke et al., 2000; Strotmann et al., 2000; Watanabe et al., 2002; Vriens et al., 2004; Loukin et al., 2010a). A decrease in extracellular osmolarity induces Ca2+ influx through TRPV4 channels (Güler et al., 2002; Liedtke and Friedman, 2003; Raoux et al., 2007; Phan et al., 2009). To determine whether hypotonicity modulates [Ca2+]RGCs, cells were exposed to saline solutions with osmolarity reduced from 280 to 190 mOsm. As illustrated in Figure 6A for an RGC loaded with the Ca2+-insensitive cell-volume indicator dye calcein AM, a reduction in osmolarity of the superfusing saline from 280 to 192 mOsm saline evoked sustained swelling of the cell. The resulting increase in cell volume was detected as a decrease in the intensity of calcein fluorescence (Fig. 6A, green trace). In contrast to hypotonic stimuli, no change in intracellular volume was observed during exposure to 100 μm glutamate.. Cell ...
Fluorescent Dyes , Fluorescent Physiological and Neurological Indicators , Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO; Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability. In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells. Hela cells stained with AnaSpec s Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204).; MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# AS-89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.; C46H46N2O23
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Determination of β-glucan in carbohydrates was studied by detecting change of exciting wavelength and emission wavelength as sodium fluorescein was mixed with β-glucan, glucose, fructose and maltose. The results showed that if sodium fluorescein was mixed with β-glucan, UV-absorption of fluorescein sodium changed from 436.5 nm to 510.6 nm, fluorescence absorption also changed: exciting wavelength from 487.3 nm to 475.9 nm, emission wavelength from 517.6 nm to 510.6 nm, if sodium fluorescein was mixed with other carbohydrates such as glucose, fructose or maltose, no change happened for fluorescence absorption. The optimal proportion of sodium fluorescein and β-glucan was 6:1, the linear concentration range was 0.1 μg/mL~0.25 μg/mL, the regression equation: y = -2.014x + 1.075 7,R2 = 0.997 8, the purity of β-glucan in raw material was 95 % using such equation, comparing with 96 % by standard enzyme method.
China Food and Drug Administration (CFDA) has released a handful of draft documents about clinical trials of medical devices. The documents, which detail the process for on-site inspections and the products that are exempt from studies, continue the ongoing overhaul of the regulation of medical devices in China.. CFDA released the documents in separate posts that went out within days of each other. The first post covered three draft documents about inspections of medical device clinical trials. In one of the documents, CFDA provides a long list of points regulatory officials should check when inspecting the conduct of medical device clinical trials. Most of the points, such as whether the informed consent process meets the relevant requirements, are applicable to all clinical research programs. Others are more specific to the conduct of clinical trials of medical devices.. The regulator released the checklist alongside two other draft documents. One of the texts, referred to as annex 3, details ...
Ocular Albumin Fluorophotometric Quantitation of Endotoxin-Induced Vascular Permeability: Bacterial endotoxin (lipopolysaccharide; LPS) is known to alter system
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Anti-Goat IgG F(ab)2 (Fluorescein Conjugated) Secondary Antibody, Rabbit Polyclonal, Fluorescein (FITC) validated in IF, FC (ASR3361), Abgent
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In the present experiments on microdissected tubules of rabbit kidney we present a refined stop-flow method for determining the rate of HCO3- absorption (J(HCO3)) or H+ secretion (JH) that can be applied to isolated microperfused tubules. Using the pH-sensitive indicator dye BCECF (2,7-bis [2-carboxyethyl]-5[6]-carboxyfluorescein) the luminal perfusate pH is continuously ...
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Mesenchymal stem cells (MSCs) have been shown to alleviate acute lung injury (ALI) and induce the production of regulatory dendritic cells (DCregs), but the potential link between these two cell types remains unclear. The goal of this study was to investigate the effect and mechanism of MSC-induced regulatory dendritic cells in ALI mice. In vivo experiments, C57BL/6 wild-type male mice were sacrificed at different times after intratracheal injection of LPS to observe changes in lung DC maturation and pathological damage. MSCs, DCregs or/and carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled DCs were administered to the mice by tail vein, and flow cytometry was performed to measure the phenotype of lung DCs and T cells. Lung injury was estimated by the lung wet weight/body weight ratio and histopathological analysis. In vitro, Western blotting or flow cytometry was used to detect the expression of Notch ligand or receptor in MSCs or DCs after coculture or LPS stimulation. Finally, in vivo and
Eukaryotic cells have a protective plasma membrane, which restricts the free movement of molecules from the external environment to the internal environment. This study aims to computationally model the transport of fluorescein derivatives across the monolayer of human brain microvascular endothelial cells (HBMEC). The determination of plausible effective diffusion constants (Deff) will allow models to be built that could be useful beyond in vitro experimentation. Fluorescein-5-isothiocyanate (FITC) modeling produced a Deff range of 1E-20 to 5E-20 cm2/s at a 1 µm cell monolayer thickness and a Deff constant near 5E-29 cm2/s at a 5 µm cell monolayer thickness. Both fluorescein and sodium fluorescein (NaFl) modeling at the 1 and 5 µm thicknesses did not produce simulations that closely resembled the HBMEC in vitro model. Overall, it is possible that the fluorescent intensity noted with fluorescein and NaFl may be better explained by a mechanism other than passive diffusion. Simulations of FITC
0148] Likewise, at least for the microspheres used in incubation step (a), the signal precursor molecules may be low molecular weight substances selected from the group consisting of fluorophores and their derivatives, luminophores and their derivatives, chromophores and their derivatives, prosthetic groups, or redox active substances selected from redox mediators, electrode-active substances. Preferably, low molecular weight signal precursor molecules are fluorophores such as fluoresceins, cyanines, carbocyanines, rhodamines, xanthenes, diazo-dye based fluorescent substances, and small fluorescent aromatic and heteroaromatic molecules. Alternatively, the low molecular weight signal precursor molecules may be chromophores such as pyrazolone, anthraquinone, carotenoid and diazo- and monoazo, oxazine, indigoid, or riboflavine based dye substances. Most preferably, the low molecular weight signal precursor molecules are fluorescein derivatives such as fluorescein diacetate (FDA), fluorescein ...
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Colicin E1 induces the efflux of carboxyfluorescein and calcein from liposomes whose phospholipid composition is similar to that of Escherichia coli. This colicin action takes place at protein-to-liposome ratios and within pH ranges that are physiologically meaningful. Colicin-induced permeability of carboxyfluorescein is not limited to the initial phase of colicin membrane interaction but is sustained thereafter. Colicin E1 requires negatively charged phospholipids in the liposomal membrane in order to bind and induce efflux.
We have developed a quantitative assay to monitor the oxidative burst (H2O2 production) of polymorphonuclear leukocytes (PMNL) using single cell analysis by flow cytometry, and have examined whether PMNL respond to membrane stimulation with an all-or-none oxidative burst. During incubation with normal neutrophils, dichlorofluorescin diacetate diffused into the cells, was hydrolyzed to 2,7-dichlorofluorescin (DCFH) and was thereby trapped within the cells. The intracellular DCFH, a nonfluorescent fluorescein analogue, was oxidized to highly fluorescent 2,7-dichlorofluorescein (DCF) by PMNL stimulated by phorbol myristate acetate (PMA). That the oxidative product was DCF was shown by excitation/emission spectra and by mass spectrometry of the product from PMA-stimulated PMNL. Normal resting and PMA-stimulated PMNL oxidized 6.9 +/- 0.7 and 160 +/- 13 attomoles DCF per cell, respectively, in 15 min. Absence of calcium and magnesium ions and/or addition of 2 mM EDTA did not inhibit DCF formation ...
Sodium fluorescein leakage for mono-, co-, and tetraculture in transwell inserts with 0.4, 1 and 3 μm pore size. Medium containing 10 µg/mL of sodium fluores
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The first step in determining whether a fluorescent dye can be used for antibody labeling consists in collecting data on its physical interaction with the latter. In the present study, the interaction between the 2-(2-hydroxy-5-nitrobenzylidene)-1,3-indanedione (HNBID) dye and the IgG1 monoclonal mouse antibody anti-human heart fatty acid binding protein (anti-hFABP) has been investigated by fluorescence and circular dichroism spectroscopies and complementary structural results were obtained by molecular modeling. We have determined the parameters characterizing this interaction, namely the quenching and binding constants, classes of binding sites, and excited state lifetimes, and we have predicted the localization of HNBID within the Fc region of anti-hFABP. The key glycosidic and amino acid residues in anti-hFABP interacting with HNBID have also been identified. A similar systematic study was undertaken for the well-known fluorescein isothiocyanate fluorophore, for comparison purposes. Our results
The CFDA has entered into an alliance with the countrys tax department to set up a social credit system. CFDA is presenting the credit system as a way to build a more complete picture of the corporate integrity of the businesses it regulates, a resource it sees as helping focus its efforts on high-risk organizations.. By partnering with the Chinese state tax administration, CFDA is looking to gain access to resources and capabilities to set up and build out the database of drug company credit ratings it proposed late last year. CFDA will work with the tax department to set up a credit information sharing platform. The system will enable the two organizations to pool information on companies. As well as the financial information associated with credit scores in the West, the CFDA database will include details of the regulatory status of each company.. Full details of what CFDA plans to track are yet to emerge. In the government briefing document that outlined the social credit system, the ...
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Martin Hughes (Bioc) (mjgh at uk.ac.cam.bio.mbfs) wrote: : ,In article ,26lcir$n6j at bigboote.WPI.EDU, eeyore at wpi.WPI.EDU ( eeYORE ) writes: : ,,Subject: viability assays : , : [about wanting a viability assay for roots : ,,I have tried FDA (fluorescein diacetate) stain, but this is not quantitative : ,,or reliable for plant tissue. Anyone have any ideas or experience? : ,, Melissa : I would also like to add a request. I have been using FDA to assay : for viability in protoplasts, which is a normal recomended method. : I am trying (as a control) to kill off protoplasts. Unfortunately, : follwing a death treatment (30 min in 0.1% NaAzide, or 0.3% KCyanide)- : which I imagine should be pretty fatal to the cells, the FDA stain : still shows positive (this is also the case with even higher toxin : levels). Any suggestions for a more sensitive method of viability : staining would be appreciated. I used neutral red which is accumulated in the vacuoles of living cells with a characteristic ...
1DZG: The Conformational Activation of Antithrombin. A 2. 85-A Structure of a Fluorescein Derivative Reveals an Electrostatic Link between the Hinge and Heparin Binding Regions.
1DZH: The Conformational Activation of Antithrombin. A 2. 85-A Structure of a Fluorescein Derivative Reveals an Electrostatic Link between the Hinge and Heparin Binding Regions.
Electroporation affects the transfer of fluorescein through inner membranes, but not the chorion. Embryos were incubated with 10 μg/ml fluorescein for 40 minut
Fluorescein is a fluorescent dye thats used in everything from eye exams to oceanography. Many researchers use fluorescein to...
Biomerica, Inc. (OTCBB: BMRA), a global provider of advanced diagnostic products for the early detection of medical conditions, today announced that it received two new China CFDA approvals for its Parathyroid Hormone (PTH) and Calcitonin products. The products will be distributed through a new China distribution channel unrelated to Biomericas current distribution channel in China
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This book characterize the efficiency of symplasmic transport, mechanisms of molecule passage via plasmodesmata, and the external and internal factors that regulate plasmodesmatal conductivity ...
Fluorescent Dyes , Reactive Fluorescent Dyes , 5(6)-TAMRA-X, SE; TAMRA-X contains a seven-atom aminohexanoyl spacer (known as X ) between TAMRA fluorophore and the succinimidyl ester. The X spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. We recommend this TAMRA-X derivative as the preferred dye for preparing TAMRA-labeled proteins when the fluorescence quenching of the labeling dye by protein is a serious problem.; C35H36N4O8
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Subject: Re: fluorescein > >Storage should be in the dark, to prevent photo bleaching of FITC when >samples are collected, fixed, handled - etc. After cutting sections >must be in dark after mounting with antifade mounting media. Vector >has a nice one, Vectashield Hardset. > >She can probably do all three preparations, but remember that if she >fixes in formalin or paraformaldehyde, the tissue will have some >autofluorescence. If the FITC signal is stronger, brighter than then >dimmer autofluoresence, then all should go well. In general, the >microcapsules are huge! and very bright. > >I would think ingesting FITC might damage it (digestive enzymes, etc) >but not sure, there may be some pH and enzyme considerations. Have >her contact tech services at Molecular Probes, they usually have >answers for fluorochromes. > >We have used FITC labelled microcapsules injected into bovine tonsil, >removed tissue, snap freeze and cryosectioned and fixed sections >immediately with NBF. Some autofluorescence ...
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Page contains details about fluorescein isothiocyanate-loaded targeted lipid-polymer hybrid nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Fluorescein injection, USP is a sterile solution for use intravenously as a diagnostic aid. It is a dark reddish orange solution with a pH of 8.3 to 9.8 and an osmolarity of 572 to 858mOsm/kg.. ...
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Fluorescein Conjugate. Biochim. Biophys. Acta, 1427, 33 - 43 (1999) C. Etzlstorfer, I Gutman and H. Falk, Concerning the ...
"Fluorescein eye stain". NIH. Retrieved 15 May 2012. Ruggieri, S; Frassanito, MA; Dammacco, R; Guerriero, S (2012-05-07). "Treg ...
Fluorescein angiography "Medscape: Medscape Access". medscape.com. Retrieved 2015-10-23. Hayreh, SS. "Posterior Ciliary Artery ...
With the anhydrides of dibasic acids, it yields fluoresceins. When heated with calcium chloride-ammonia to 200 °C it yields ...
"Expert panel approves fluorescein strips use". Optician Online. 25 September 2013. Retrieved 17 July 2015. "Optical ... The Confederation negotiated a solution to the difficulties posed by the use of fluorescein impregnated paper strips when ...
The defect stains brightly with fluorescein. In the very early stages the desquamation may be patchy before progressing to ...
J. Donald M. Gass, a macular degeneration specialist developed the use of fluorescein angiography as a diagnostic tool, and Dr ... Norton, EW; Gutman, F (1965). "Diabetic retinopathy studied by fluorescein angiography". Transactions of the American ... pioneered the use of fluorescein angiography for the diagnosis of macular and retinal diseases, which led to the accurate ...
Brush, C. K. "Fluorescein Labelled Phosphoramidites". (1996) U.S. Patent 5,583,236. Pitner, J. B.; Linn, C. P. "Synthesis and ... exemplified by 6-FAM amidite 7 for the attachment of fluorescein and dabcyl amidite 8, respectively), hydrophilic and ...
Fluorescein angiography is a helpful adjunct. Findings include delayed venous filling, hypofluorescence caused by hemorrhage ...
... can be labelled with fluorescein isothiocyanate. The conjugate retains its antiproteolytic and carbohydrate-binding ...
Fluorescein angiography may demonstrate leakage in areas remote from the retinal infarctions. In a recent analysis (Susac et al ... Gass Plaques and Fluorescein Leakage in Susac Syndrome. Journal of Neurological Sciences 299(1-2): 97-100; 2010 Susac JO, ... Both patients underwent fluorescein retinal angiography that demonstrated multifocal retinal artery occlusions without evidence ...
A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents ... Fluorescein Molecular Imaging Products Company (2005-08-26). "5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg ...
It is an important landmark in Fluorescein angiography. Its diameter is 0.5mm, the central 1.5 degrees of an individual's ...
j) Multiple muscular VSDs are a challenge to close, achieving a complete closure can be aided by the use of fluorescein dye. ... "Use of Fluorescein Dye to Identify Residual Defects". Ann Thorac Surg. 97 (1): e27-8. doi:10.1016/j.athoracsur.2013.10.059. ...
... labeled with the fluorochrome fluorescein isothiocyanate (FITC) to yield the fluorescein thiocarbamoyl (FTC) ... casein labeled with the fluorochrome fluorescein isothiocyanate (FITC) to yield the fluorescein thiocarbamoyl (FTC) derivative ... Ageitos, J.M.; Vallejo, J.A.; Poza, M.; Villa, T.G. (2006). "Fluorescein Thiocarbamoyl-Kappa-Casein Assay for the Specific ... InterPro: IPR000117 Kappa casein Fluorescein Thiocarbamoyl-Kappa-Casein Assay for the Specific Testing of Milk-Clotting ...
Variability in fluorescein angiography interpretation for photodynamic therapy in age-related macular degeneration. Retina. ... Quantitative image sequence analysis of fundus fluorescein angiography. Ophthalmic Surg Lasers. 1999 Jan;30(1):72-3. 19. Shin ... Quantitative, spatio-temporal image analysis of fluorescein angiography in age-related macular degeneration. Proc SPIE 1998; ... of quantitative retinal imaging with a number of publications on image analysis methods for modalities such as fluorescein ...
... Y is a tetrabromo derivative of fluorescein. Eosin B is a dibromo dinitro derivative of fluorescein. Eosin is most often ... and stains them dark red or pink as a result of the actions of bromine on fluorescein. In addition to staining proteins in the ...
Fluorescein is currently used most as a fluorescent probe. Equipment that can automatically measure and calculate the capacity ... Most of them employ the same principle (i.e. measurement of AAPH-radical mediated damage of fluorescein); however, ORAC-EPR, ... The degeneration (or decomposition) of fluorescein is measured as the presence of the antioxidant slows the fluorescence decay ... The assay measures the oxidative degradation of the fluorescent molecule (either beta-phycoerythrin or fluorescein) after being ...
Fluorescein Angiogram: evaluation of blood circulation in the retina. Dilated Pupillary Exam: special drops expand the pupil, ...
Examples of commonly used fluorophores are fluorescein or rhodamine. The antibodies can be tailor-made for a chemical compound ...
Since then, Fluorescein was created as a fluorescent dye by Adolph von Baeyer in 1871 and the method of staining was developed ... Usually fluorescein or biotin is used as the fluorophore. Chemical labeling or the use of chemical tags utilizes the ... Ethidium bromide, fluorescein and green fluorescent protein are common tags. The most commonly labelled molecules are ...
Fluorescein angiography is commonly used to diagnose the syndrome. There have been several methods in treating patients who ... Fluorescein angiography is quite useful in diagnosing the disease, and the use of ultrasonography and optical coherence ... Malformations in arteriovenous connections and irregular functions in the veins may be distinguished by fluorescein ...
A fluorescein strip containing 10% fluorescein is applied topically to the affected area and is examined with a cobalt blue ... The change in the color of the fluorescein strip is due to dilution of fluorescein caused by the aqueous leakage in the cornea ... If the fluorescein strip turns pale upon application to the corneal surface, the person tests positive for the corneal ... At this point, the fluorescein appears green in color. Any changes in color or surface of the fluorescence area indicate the ...
Fluorescein is among the first fluorescent dyes, developed in 1871. Its disodium salt under the trademark "Uranine" was ... see fluorescein in biological research). For example, the fluorescent-activated cell sorting in flow cytometry makes it ...
Fluorescein dye can aid in the localisation of chyle leak. Hypertension is defined when a patient's blood pressure in the arm ... "Intraoperative Identification of Chyle Leak During Coarctation Repair Using Fluorescein Dye". The Annals of Thoracic Surgery. ...
Menon M: The use of fluorescein to demarcate the avascular plane in anatrophic nephrolithotomy. Urology, 22:183, 1984. ... Menon, M (1984). "Use of fluorescein to demonstrate avascular plane in anatrophic nephrolithotomy". Urology. 23 (2): 183. doi: ...
The abnormal vessels are even better seen with fluorescein angiography. In advanced disease, glaucoma is diagnosed by measuring ...
It is excited at 508 nm and emits 528 nm, a green-yellow, of free fluorescein. The quantum yield is 0.49 for 250 nM FlAsH is ... The modification of the fluorescein moiety also allows multicolor analysis. It has been proven to be a good alternative to ... Its structure is based around a fluorescein core with two 1,3,2-dithiarsolane substituents. It is used in bioanalytical ... FlAsH-EDT2 can be prepared in three steps from fluorescein (see figure). Many studies show that trivalent arsenic compounds ...
Amar Agarwal (2007). Fundus Fluorescein and Indocyanine Green Angiography: A Textbook and Atlas. SLACK Incorporated. pp. 18-. ...
Unlike resorcinol it does not give a fluorescein with phthalic anhydride. Oxidation of the ammoniacal solution gives orcein, ...
One use is in determining the viability of pollen grains; grains which are alive take up the fluorescein diacet... ... One use is in determining the viability of pollen grains; grains which are alive take up the fluorescein diacetate and ... hydrolyze it intracellularly; the released fluorescein will fluoresce, and can be detected with a microscope using filters that ...
Global Fluorescein Diacetate Market Size, Status and Forecast 2021. August 2, 2017 stanley Business 0 ... The "Fluorescein Diacetate Market" report covers an in-depth study of current rules, policies, and regulations, and industrial ... The market report, titled as Fluorescein Diacetate Market, is an exploratory survey performed on the basis of global market, ... Made by using accomplished systematic techniques such SWOT analysis, the "Worldwide Fluorescein Diacetate Market" report gives ...
This helps you to get all India Fluorescein Diacetate Export data. You can find previous two days Fluorescein Diacetate Export ... LABORATORY CHEMICALS FLUORESCEIN DIACETATE USED AS CELL VIABILITY STAIN. Tamatave (Toamasina). 1. PCS. 46959.76. 46959.76. ... Fluorescein Diacetate Export data can be useful in different kind of analysis such as: Export price, Quantity, market scenarios ... Export Data And Price Of Fluorescein Diacetate , www.eximpulse.com Eximpulse Services is the place where you can find the ...
Antonyms for direct fluorescein-conjugated antibody. 21 words related to antibody: active site, protein, autoantibody, ... What are synonyms for direct fluorescein-conjugated antibody? ... Synonyms for direct fluorescein-conjugated antibody in Free ... redirected from direct fluorescein-conjugated antibody). Also found in: Dictionary, Medical, Encyclopedia. #vtZoom,.vt-link{ ... Direct fluorescein-conjugated antibody synonyms, direct fluorescein-conjugated antibody antonyms - FreeThesaurus.com https:// ...
Chemical derivatives of fluorescein: Eosin Carboxyfluorescein Fluorescein isothiocyanate (FITC) Fluorescein amidite (FAM) ... Fluorescein is also known as a color additive (D&C Yellow no. 7). The disodium salt form of fluorescein is known as uranine or ... Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and ... Fluorescein has an isosbestic point (equal absorption for all pH values) at 460 nm. There are many fluorescein derivatives. For ...
Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons ... Fluorescein amidite, abbreviated as FAM (commercially available 6-FAM version is shown in Figure), is an important synthetic ... FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the ... Brush, C. K. Fluorescein Labelled Phosphoramidites. US Patent 5,583,236. [1]. ...
... is a γ-lactone (CHEBI:37581) fluorescein (CHEBI:31624) is a 2-benzofurans (CHEBI:38831) fluorescein ( ... fluorescein (CHEBI:31624) is a polyphenol (CHEBI:26195) fluorescein (CHEBI:31624) is a xanthene dye (CHEBI:37929) ... fluorescein (CHEBI:31624) has role fluorescent dye (CHEBI:51121) fluorescein (CHEBI:31624) has role radioopaque medium (CHEBI: ... 5-CM-H2DCFDA (CHEBI:90122) has functional parent fluorescein (CHEBI:31624). 6-O-(carboxymethyl)fluorescein ethyl ester (CHEBI: ...
... (FFA). This is a special test used for examination of blood vessels in the eye. It is an office ... Fluorescein Angiography may be done when any retinal disease, especially that involving the blood vessels is suspected, like ... A bright yellow- green dye, called fluorescein is injected into a vein in the arm. Various photos of the blood vessels are ...
Fluorescein is used in medicine to diagnose corneal ulcers and certain other eye disorders. A solution of the chemical is ... fluorescein isothiocyanate is a fluorescent chemical derived from fluorescein which is used to label proteins. fluorescein- ... Fluorescein diacetate. fluorescence immunoassay. fluorescent label. Clone High. Ponce de Leon. Aquaplaning. grid reference. ... Fluorescein is used in medicine to diagnose corneal ulcers and certain other eye disorders. A solution of the chemical is ...
Minims fluorescein are single-use preservative-free eye drops that contain the active ingredient fluorescein. This is a ... Minims fluorescein are single-use preservative-free eye drops that contain the active ingredient fluorescein. This is a ... Fluorescein can also be used to find foreign bodies such as grit in the eye, as they show up surrounded by a green ring. ... Fluorescein can only enter damaged cells of the eye. It will temporarily stain any cells it enters and therefore marks any ...
... fluorescein) and a blue light to detect foreign bodies in the eye. This test can also detect damage to the cornea. The cornea ... This is a test that uses orange dye (fluorescein) and a blue light to detect foreign bodies in the eye. This test can also ...
fluorescein synonyms, fluorescein pronunciation, fluorescein translation, English dictionary definition of fluorescein. n. An ... Related to fluorescein: fluorescein isothiocyanate, Fluorescein sodium. fluo·res·ce·in. (flo͝o-rĕs′ē-ĭn, flô-, flō-). n.. An ... fluorescein. (ˌflʊəˈrɛsɪɪn) or fluoresceine. n. (Elements & Compounds) an orange-red crystalline compound that in aqueous ... Fluorescein staining is enhanced with cell degeneration or cell death, which increases membrane permeability to fluorescein.. ...
Fluorescein angiogram of the left eye. a. This image shows an arterial phase of the dye, with areas of hypofluorescence ... Fluorescein angiogram of the left eye. a. This image shows an arterial phase of the dye, with areas of hypofluorescence ...
Fluorescein - Generated on July 19, 2019. ©2019 Memorial Sloan Kettering Cancer Center. ...
... is based on dipivaloylfluorescein and is suitable for standard fluorescein filter sets ... Fluorescein is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells or in ... Figure 3. Structure of CLIP-Cell Fluorescein (MW 756.8 g/mol).. Product Categories:. Discontinued Products. * Properties & ... CLIP-Cell™ Fluorescein is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells ...
... fluorescein sodium injection, solution), including warnings and precautions, directions, and the names of other drugs and ...
Fluorescein is a fluorescent dye thats used in everything from eye exams to oceanography. Many researchers use fluorescein to ... Several chemical companies manufacture fluorescein and derivatives of this compound, such as fluorescein isothiocyanate. These ... Is fluorescein detection possible with the naked eye, or do the molecules only glow in the dark or under something like a dark ... In pure state, fluorescein is a red or orange powder which is dissolved in alkaline solutions to make up a yellow solution ...
Fluorescein) may treat, side effects, dosage, drug interactions, warnings, patient labeling, reviews, and related medications ... FLUORESCITE® (fluorescein injection, USP) 10% contains fluorescein sodium (equivalent to fluorescein 10% w/v). It is a sterile ... Fluorescein undergoes rapid metabolism to fluorescein monoglucuronide. After IV administration of fluorescein sodium (14 mg/kg ... Single use 5 mL vial containing 100 mg/mL fluorescein.. Storage And Handling. FLUORESCITE® (fluorescein injection, USP) 10% is ...
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A Moderate Drug Interaction exists between fluorescein / proparacaine ophthalmic and tafenoquine. View detailed information ... Drug Interactions between fluorescein / proparacaine ophthalmic and tafenoquine. This report displays the potential drug ...
Fluorescein injection is used to help certain parts of the eye (eg, retina, iris) become more visible during eye medical ...
fluorescein. fluorescein isocyanate. fluorescein isothiocyanate. fluoresceine. fluorescence. fluorescence microscopy. ... turns pink when no chloride ions are left in solution and negative fluorescein ions are then absorbed). - fluoresceine, ... Noun: fluorescein flûre-see-un [N. Amer], flo-ru,seen or flûr-u,seen [Brit]. *(solution) a yellow dye that is visible even ...
Fluorescein Lite. Fluorescein sodium (100 mg/1mL). Injection, solution. Intravenous. Altaire Pharmaceuticals Inc.. 2001-10-01. ... Fluorescein Lite. Fluorescein sodium (250 mg/1mL). Injection, solution. Intravenous. Altaire Pharmaceuticals Inc.. 2002-01-01. ... Minims Lidocaine and Fluorescein. Fluorescein sodium (0.25 %) + Lidocaine hydrochloride (4 %). Solution / drops. Ophthalmic; ... The excretion of Fluorescein can be decreased when combined with Acetaminophen.. Acetazolamide. The excretion of Fluorescein ...
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.1,2,3 Conflicting data on the branch lengths implies that the average branch length is less than three glucose units.4,5 However, other methods indicate branches of greater than 50 glucose units exist.6,7 Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.8,9,10 Lower MW dextrans will exhibit slightly less branching4 and have a more narrow range of MW distribution.11 Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry.7,12,13 Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil.12 At MW below 2,000, dextran is more rod-like.14 The MW of dextran is measured by one or more ...
Rabbit polyclonal Fluorescein antibody validated for WB, ELISA. Referenced in 8 publications. Immunogen corresponding to ... Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to ... Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and ... Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 ...
Fluorescein Injection) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and ... AK-FLUOR® (Fluorescein Injection, USP) is a sterile solution in Water for Injection, of Fluorescein prepared with the aid of ... Fluorescein has been demonstrated to be excreted in human milk. Caution should be exercised when AK-FLUOR®(Fluorescein ... AK-FLUOR®, 10% (Fluorescein Injection, USP - Sterile) 100 mg/mL. NDC 17478-253-10 5 mL single dose vials in packs of 12. NDC ...
Fluorescein conjugated/tagged from R&D Systems,Request Info,biological,biology supply,biology supplies,biology product ... ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene. 8. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent ... CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON. 11. ApopTag Fluorescein Direct In Situ Apoptosis Detection ... Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio ...
Shop a large selection of Tertiary Colors Stains Dyes and Indicators products and learn more about Fluorescein, disodium salt, ...
Fluorescein diacetate, 97%, pure, ACROS Organics 5g; Glass bottle Chemicals:Organic Compounds:Organoheterocyclic compounds: ... fluorescein diacetate,3,6-diacetoxyfluoran,diacetylfluorescein,di-o-acetylfluorescein,fluorescein, diacetate,3,6- ... fluorescein diacetate,3,6-diacetoxyfluoran,diacetylfluorescein,di-o-acetylfluorescein,fluorescein, diacetate,3,6- ...
Anti-Fluorescein antibody conjugated to Texas Red ® validated for WB, ELISA, Flow Cyt, ICC/IF. Referenced in 1 publication and ... Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to ... Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and ... Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 ...
  • Eximpulse Services is the place where you can find the recent and updated Trade intelligence report of Fluorescein Diacetate Export Data. (eximpulse.com)
  • This helps you to get all India Fluorescein Diacetate Export data. (eximpulse.com)
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  • In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. (abcam.com)
  • Maximum fluorescence occurs at a pH of 7.4, but the pH of fluorescein sodium for angiographic use is adjusted to a range of 8 to 9.8 for stability. (opsweb.org)
  • Fluorescein-DHPE (N-(Fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) is a useful surface pH indicator (1) and has also been used for measuring lateral diffusion in membranes via fluorescence photobleaching recovery method. (biotium.com)
  • Changes in the cytoplasmic matrix were measured by means of intracellular fluorescein fluorescence polarization (IFFP) using the Cellscan apparatus. (spiedigitallibrary.org)
  • Dror Fixler , Reuven Tirosh, Avi Eisenthal, Shlomo Lalchuk, Oleg Marder, Yosef Irlin, Mordechai Deutsch, "Prelytic stimulation of target and effector cells following conjugation as measured by intracellular fluorescein fluorescence polarization," Journal of Biomedical Optics 3(3), (1 July 1998). (spiedigitallibrary.org)
  • This is a technique that enables the observation of the vessels of the retina using a series of photographs taken after an intravenous injection of fluorescein. (specsavers.co.uk)
  • The intravenous injection of fluorescein is done just after having changed the color camera by the black and white one. (vin.com)
  • This diagnostic procedure utilizes a specialized fundus camera or scanning laser ophthalmoscope to capture rapid-sequence photographs of the retina following an intravenous injection of fluorescein sodium. (opsweb.org)
  • It is a test that involves the intravenous injection of fluorescein dye, followed by imaging of the dye's passage through the blood vessels inside the eye. (clinicaltrials.gov)
  • Patients having retinal detachment, tractional or rhegmatogenous, vitreous haemorrhage and macular surface disorders (assessed with slit lamp biomicroscope and funds condensing lenses, IOP was measured with AT and fluorescein ) were included. (thefreedictionary.com)
  • Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-17F Fluorescein-conjugated Monoclonal Antibody. (antibodies-online.com)
  • Using highly optimized microwave based solid-phase chemistry a series of fluorescein-labelled cationic peptoid conjugates were synthesized within 24h and cellular uptake into HeLa, L929 and K562 cells examined via flow cytometry. (biomedsearch.com)
  • In addition to using straight fluorescein, researchers also work with various derivatives of the compound which are designed to conjugate to specific types of molecules and perform other functions. (wisegeek.com)
  • The green-fluorescent fluorescein heparin conjugate should be a useful tool for studying binding of this mucopolysaccharide in cells and tissue. (thermofisher.com)
  • 2 It is the water-soluble salt of fluorescein, also known as resorcinolphthalein sodium, or uranine. (opsweb.org)
  • Fluorescein is a fluorescent dye which is used in a number of different applications in the sciences and medicine. (wisegeek.com)
  • The fluorescent dye used is sodium fluorescein injected intra venously. (vin.com)
  • Fluorescein sodium was the first fluorescent dye used for water tracing purposes. (opsweb.org)
  • Bilateral fluorescein angiographic findings in unilateral Coats' disease. (biomedsearch.com)
  • Figure 1: Ultra-widefield oral fluorescein angiographic image (left eye) demonstrating peripheral vascular telangiectasias and leakage in a 6-year-old boy with Coats' Disease. (arvojournals.org)
  • Figure 2: Ultra-widefield oral fluorescein angiographic image (right eye) demonstrating mid-peripheral neovascularization and avascular peripheral retina in a 12-year-old girl with Sickle Cell Retinopathy. (arvojournals.org)
  • FLUORESCITE ® (fluorescein injection, USP) 10% contains fluorescein sodium (equivalent to fluorescein 10% w/v). It is a sterile solution for use intravenously as a diagnostic aid. (rxlist.com)
  • FLUORESCITE ® (fluorescein injection, USP) 10% is supplied as a sterile, unpreserved, unit dose aqueous solution, that has a pH of 8.0 - 9.8 and an osmolality of 572-858 mOsm/kg. (rxlist.com)
  • If the needle has extravasated, the patient's blood will be seen to bulge the skin and the injection should be stopped before any fluorescein is injected. (rxlist.com)
  • When assured that extravasation has not occurred, the room light may be turned off and the fluorescein injection completed. (rxlist.com)
  • FLUORESCITE ® (fluorescein injection, USP) 10% is supplied in a single use 5 mL glass vial with a gray FluroTec coated chlorobutyl stopper and purple flip-off aluminum seal. (rxlist.com)
  • Fluorescein injection is used to help certain parts of the eye (eg, retina, iris) become more visible during eye medical procedures. (mayoclinic.org)
  • AK-FLUOR® (Fluorescein Injection, USP) is a sterile solution in Water for Injection, of Fluorescein prepared with the aid of Sodium Hydroxide. (rxlist.com)
  • AK-FLUOR® (fluorescein injection) is used intravenously as a diagnostic aid. (rxlist.com)
  • A syringe, filled with fluorescein, is attached to transparent tubing and a 25 gauge scalp vein needle for injection. (rxlist.com)
  • Care must be taken to avoid extravasation during injection as the high pH of fluorescein solution can result in severe local tissue damage. (rxlist.com)
  • Hey W. Injection of fluorescein in the study of bladder tumors. (jaoa.org)
  • The stain caused by fluorescein differs according to the part of the eye affected. (netdoctor.co.uk)
  • One of the most common tests done if a corneal ulcer is suspected is a fluorescein stain. (thefreedictionary.com)
  • In fact, its common use in industrial plumbing led a plumbers union to start the tradition of using fluorescein to stain the Chicago River green for the annual St. Patrick's Day celebration. (opsweb.org)
  • 5-7 In ophthalmology, topical application of fluorescein sodium is routinely used for applanation tonometry, and as a vital stain in the documentation of ocular surface disorders such as corneal ulcers, abrasions, or other epithelial defects. (opsweb.org)
  • Fluorescein angiogram of the left eye. (aao.org)
  • I am very allergic to this compound and am due to have a fluorescein angiogram in a few weeks. (wisegeek.com)
  • Montage Fluorescein Angiogram Images. (healio.com)
  • Left) Fluorescein Angiogram of the Right Eye Demonstrating Peripheral Avascular Retina Bordered Posteriorly by Vessels Running Parallel to the Ora Serrata (arrows). (healio.com)
  • FLUORESCEIN ANGIOGRAM OF EYE Photo essay at the hospital of Meaux (77), France. (sciencephoto.com)
  • Fluorescein Sodium and Proparacaine Hydrochloride Ophthalmic Solution, USP (Sterile) is a sterile ophthalmic solution combining the disclosing action of Fluorescein with the anesthetic action of Proparacaine Hydrochloride. (nih.gov)
  • Many of the medical and ophthalmic applications of fluorescein are analogous to its uses in plumbing or industrial flow dynamics. (opsweb.org)
  • The disodium salt form of fluorescein is known as uranine or D&C Yellow no. 8. (wikipedia.org)
  • The active ingredient, Fluorescein Sodium , has the chemical name Spiro [isobenzofuran-1 ( 3 H) , 9'-[9 H ]xanthene]-3-one, 3' ,6' dihydroxy-,disodium salt. (nih.gov)
  • In previous studies micronized disodium fluorescein (DF) powders were coated with lauric or capric acid. (rti.org)
  • Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a diagnostic tool in the field of ophthalmology and optometry, where topical fluorescein is used in the diagnosis of corneal abrasions, corneal ulcers and herpetic corneal infections. (wikipedia.org)
  • GFP Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. (rockland-inc.com)
  • Fluorescein-Conjugated Goat Anti Mouse IgG is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. (mpbio.com)
  • Appointments for the above testing procedures can be made by contacting the Fluorescein Lab in the Retina Service at Mass. Eye and Ear at 617-573-3577 . (masseyeandear.org)
  • Other uses of fluorescein include using it as a water-soluble dye added to rainwater in environmental testing simulations to aid in locating and analyzing any water leaks, and in Australia and New Zealand as a methylated spirit dye. (wikipedia.org)
  • Fluorescein labeled lectin has an excitation maximum at 495 nm and an emission maximum at 515 nm. (vectorlabs.com)
  • Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. (abcam.com)
  • This lectin screening kit is designed to provide the investigator with a panel of seven fluorescein labeled lectin conjugates. (vectorlabs.com)
  • Synthesis, penetrability and intracellular targeting of fluorescein-tagged peptoids and peptide-peptoid hybrids. (biomedsearch.com)
  • Axonal guidance mutants of Caenorhabditis elegans identified by filling sensory neurons with fluorescein dyes. (nih.gov)
  • Eight pairs of chemosensory neurons in Caenorhabditis elegans take up fluorescein dyes entering through the chemosensory organs. (nih.gov)
  • The Alexa Fluor ® , Cascade Blue ® , lucifer yellow, fluorescein, and Oregon Green ® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red ® dyes are essentially neutral. (thermofisher.com)
  • Water resource agencies and groundwater scientists use fluorescein dyes to trace ground water flows that supply surface waters that may contain threatened or endangered mollusk species. (usgs.gov)
  • When an eye with fluorescein drops in is exposed to blue light, the fluorescein also gives a bright greenish yellow glow to the entire corneal surface. (netdoctor.co.uk)
  • Fluorescein drops are placed onto the surface of the eye to detect ulcers of the cornea or damage to the corneal surface. (viovet.co.uk)
  • The fluorescein helps the optometrist perform the exam, while the lidocaine numbs the eye to stop you feeling anything. (netdoctor.co.uk)
  • Disclaimer: This information is independently developed by MIMS based on Lidocaine + Fluorescein from various references and is provided for your reference only. (mims.com)
  • Fluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. (wikipedia.org)
  • Additional biologically active molecules (such as antibodies) may also be attached to fluorescein, allowing biologists to target the fluorophore to specific proteins or structures within cells. (wikipedia.org)
  • Fluorescein-labelled probes can be imaged using FISH, or targeted by antibodies using immunohistochemistry. (wikipedia.org)
  • FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the complementary nucleic acids or primers for polymerase chain reaction. (wikipedia.org)
  • Our findings show the value of fluorescein analogues as probes for mechanistic studies of SecA functions and for the potential development of new antimicrobial agents with SecA as the target. (wiley.com)
  • Fluorescein is a diagnostic dye. (mims.com)
  • The use of fluorescein amidite shown above allows one to synthesize labeled oligonucleotides for the same purpose. (wikipedia.org)
  • Fluorescein amidite, abbreviated as FAM (commercially available 6-FAM version is shown in Figure), is an important synthetic equivalent of fluorescein dye used in oligonucleotide synthesis and molecular biology. (wikipedia.org)
  • Yet another technique termed molecular beacons makes use of synthetic fluorescein-labeled oligonucleotides. (wikipedia.org)
  • Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons. (wikipedia.org)
  • Fluorescein is used in medicine to diagnose corneal ulcer s and certain other eye disorders. (everything2.com)
  • The Trans-Epithelial Permeability (TEP) Assay, is a cell-based assay used to evaluate the potential ocular irritancy of test chemicals by measuring the permeability of sodium fluorescein (or fluorescein leakage) through a confluent monolayer of Madin-Darby Canine Kidney (MDCK) cells. (iivs.org)
  • In addition, fluorescein derivatives are used in the lab to identify compounds in fluid samples, with the chemical attaching to specific proteins to act as a flag. (wisegeek.com)
  • CLIP-Cell™ Fluorescein is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells or in vitro . (neb.com)
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. (rockland-inc.com)
  • The ocular structures impregnated by fluorescein are illuminated by a blue radiation which makes them luminescent. (vin.com)