Fluorescein
Fluoresceins
Fluorescein Angiography
Fluorescein-5-isothiocyanate
Fluorophotometry
Fundus Oculi
Fluorescent Dyes
Choroidal Neovascularization
Choroid
Dextrans
Dry Eye Syndromes
Corneal and conjunctival dryness due to deficient tear production, predominantly in menopausal and post-menopausal women. Filamentary keratitis or erosion of the conjunctival and corneal epithelium may be caused by these disorders. Sensation of the presence of a foreign body in the eye and burning of the eyes may occur.
Vitreous Body
Indocyanine Green
Laser Coagulation
Visual Acuity
Clarity or sharpness of OCULAR VISION or the ability of the eye to see fine details. Visual acuity depends on the functions of RETINA, neuronal transmission, and the interpretative ability of the brain. Normal visual acuity is expressed as 20/20 indicating that one can see at 20 feet what should normally be seen at that distance. Visual acuity can also be influenced by brightness, color, and contrast.
Rose Bengal
Rhodamines
Capillary Permeability
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
Choroid Diseases
Macular Degeneration
Microscopy, Fluorescence
Macula Lutea
An oval area in the retina, 3 to 5 mm in diameter, usually located temporal to the posterior pole of the eye and slightly below the level of the optic disk. It is characterized by the presence of a yellow pigment diffusely permeating the inner layers, contains the fovea centralis in its center, and provides the best phototropic visual acuity. It is devoid of retinal blood vessels, except in its periphery, and receives nourishment from the choriocapillaris of the choroid. (From Cline et al., Dictionary of Visual Science, 4th ed)
Fluorometry
Tomography, Optical Coherence
Cornea
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Ophthalmic Solutions
Blood-Retinal Barrier
Erythrosine
Macular Edema
Fluid accumulation in the outer layer of the MACULA LUTEA that results from intraocular or systemic insults. It may develop in a diffuse pattern where the macula appears thickened or it may acquire the characteristic petaloid appearance referred to as cystoid macular edema. Although macular edema may be associated with various underlying conditions, it is most commonly seen following intraocular surgery, venous occlusive disease, DIABETIC RETINOPATHY, and posterior segment inflammatory disease. (From Survey of Ophthalmology 2004; 49(5) 470-90)
Permeability
Eosine I Bluish
Diabetic Retinopathy
Retina
The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.
Retinal Artery
Coloring Agents
Rabbits
Retinal Vein Occlusion
Anterior Chamber
The space in the eye, filled with aqueous humor, bounded anteriorly by the cornea and a small portion of the sclera and posteriorly by a small portion of the ciliary body, the iris, and that part of the crystalline lens which presents through the pupil. (Cline et al., Dictionary of Visual Science, 4th ed, p109)
Staining and Labeling
Photography
Microscopy, Confocal
Fluorescence
Retinal Vein
Retinal Artery Occlusion
Light Coagulation
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Retinal Neovascularization
Fovea Centralis
An area approximately 1.5 millimeters in diameter within the macula lutea where the retina thins out greatly because of the oblique shifting of all layers except the pigment epithelium layer. It includes the sloping walls of the fovea (clivus) and contains a few rods in its periphery. In its center (foveola) are the cones most adapted to yield high visual acuity, each cone being connected to only one ganglion cell. (Cline et al., Dictionary of Visual Science, 4th ed)
Sclera
The white, opaque, fibrous, outer tunic of the eyeball, covering it entirely excepting the segment covered anteriorly by the cornea. It is essentially avascular but contains apertures for vessels, lymphatics, and nerves. It receives the tendons of insertion of the extraocular muscles and at the corneoscleral junction contains the canal of Schlemm. (From Cline et al., Dictionary of Visual Science, 4th ed)
Intravitreal Injections
Aqueous Humor
Retinal Detachment
Separation of the inner layers of the retina (neural retina) from the pigment epithelium. Retinal detachment occurs more commonly in men than in women, in eyes with degenerative myopia, in aging and in aphakia. It may occur after an uncomplicated cataract extraction, but it is seen more often if vitreous humor has been lost during surgery. (Dorland, 27th ed; Newell, Ophthalmology: Principles and Concepts, 7th ed, p310-12).
Lasers
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
Iris
Lissamine Green Dyes
Conjunctiva
Pigment Epithelium of Eye
Flow Cytometry
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Fluorescence Polarization
Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
Photochemotherapy
Papilledema
Swelling of the OPTIC DISK, usually in association with increased intracranial pressure, characterized by hyperemia, blurring of the disk margins, microhemorrhages, blind spot enlargement, and engorgement of retinal veins. Chronic papilledema may cause OPTIC ATROPHY and visual loss. (Miller et al., Clinical Neuro-Ophthalmology, 4th ed, p175)
Epithelium, Corneal
Blood-Aqueous Barrier
Central Serous Chorioretinopathy
Hydrogen-Ion Concentration
Ophthalmoscopes
Keratoconjunctivitis Sicca
Retinal Vasculitis
Probenecid
The prototypical uricosuric agent. It inhibits the renal excretion of organic anions and reduces tubular reabsorption of urate. Probenecid has also been used to treat patients with renal impairment, and, because it reduces the renal tubular excretion of other drugs, has been used as an adjunct to antibacterial therapy.
Microscopy, Ultraviolet
Energy Transfer
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
Cerebrospinal Fluid Rhinorrhea
Extravasation of Diagnostic and Therapeutic Materials
Diffusion
Meibomian Glands
Cell Membrane Permeability
Telangiectasis
Drug Delivery Systems
Retinal Drusen
Phycoerythrin
Photosensitizing Agents
Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue. These compounds can be administered topically or systemically and have been used therapeutically to treat psoriasis and various types of neoplasms.
Retinal Pigment Epithelium
The single layer of pigment-containing epithelial cells in the RETINA, situated closely to the tips (outer segments) of the RETINAL PHOTORECEPTOR CELLS. These epithelial cells are macroglia that perform essential functions for the photoreceptor cells, such as in nutrient transport, phagocytosis of the shed photoreceptor membranes, and ensuring retinal attachment.
Biological Transport
Choroid Neoplasms
Chorioretinitis
Exudates and Transudates
Exudates are fluids, CELLS, or other cellular substances that are slowly discharged from BLOOD VESSELS usually from inflamed tissues. Transudates are fluids that pass through a membrane or squeeze through tissue or into the EXTRACELLULAR SPACE of TISSUES. Transudates are thin and watery and contain few cells or PROTEINS.
Administration, Topical
The application of drug preparations to the surfaces of the body, especially the skin (ADMINISTRATION, CUTANEOUS) or mucous membranes. This method of treatment is used to avoid systemic side effects when high doses are required at a localized area or as an alternative systemic administration route, to avoid hepatic processing for example.
Lacrimal Apparatus
Cells, Cultured
Haptens
Benzalkonium Compounds
Binding Sites
The bystander effect in the HSVtk/ganciclovir system and its relationship to gap junctional communication. (1/2851)
The bystander effect (BSE) is an interesting and important property of the herpes thymidine kinase/ganciclovir (hTK/GCV) system of gene therapy for cancer. With the BSE, not only are the hTK expressing cells killed upon ganciclovir (GCV) exposure but also neighboring wild-type tumor cells. On testing a large number of tumor cell lines in vitro, a wide range of sensitivity to bystander killing was found. Since transfer of toxic GCV metabolites from hTK-modified to wild-type tumor cells via gap junctions (GJ) seemed to be a likely mechanism of the BSE, we tested GJ function in these various tumors with a dye transfer technique and pharmacological agents known to affect GJ communication. We confirmed that mixtures of tumor cell resistant to the BSE did not show dye transfer from cell to cell while bystander-sensitive tumor cells did. Dieldrin, a drug known to decrease GJ communication, diminished dye transfer and also inhibited the BSE. Forskolin, an upregulator of cAMP did increase GJ, but directly inhibited hTK and therefore its effect on BSE could not be determined. We conclude that these observations further support port the concept that functional GJ play an important role in the BSE and further suggest that pharmacological manipulation of GJ may influence the outcome of cancer therapy with hTK/GCV. (+info)Overexpression of the multidrug resistance-associated protein (MRP1) in human heavy metal-selected tumor cells. (2/2851)
Cellular and molecular mechanisms involved in the resistance to cytotoxic heavy metals remain largely to be characterized in mammalian cells. To this end, we have analyzed a metal-resistant variant of the human lung cancer GLC4 cell line that we have selected by a step-wise procedure in potassium antimony tartrate. Antimony-selected cells, termed GLC4/Sb30 cells, poorly accumulated antimony through an enhanced cellular efflux of metal, thus suggesting up-regulation of a membrane export system in these cells. Indeed, GLC4/Sb30 cells were found to display a functional overexpression of the multidrug resistance-associated protein MRP1, a drug export pump, as demonstrated by Western blotting, reverse transcriptase-polymerase chain reaction and calcein accumulation assays. Moreover, MK571, a potent inhibitor of MRP1 activity, was found to markedly down-modulate resistance of GLC4/Sb30 cells to antimony and to decrease cellular export of the metal. Taken together, our data support the conclusion that overexpression of functional MRP1 likely represents one major mechanism by which human cells can escape the cytotoxic effects of heavy metals. (+info)Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers. (3/2851)
A fluorimetric multi-parameter cell sensor at the single cell level is presented which makes it possible to observe the physiological behavior of different cell lines, different physiological parameters, and statistical data at the same time. Different cell types were immobilized at predefined positions with high accuracy using optical tweezers and adhesion promoting surface layers. The process is applicable to both adherent and non-adherent cells. Coating of the immobilization area with mussel adhesive protein was shown to be essential for the process. Intracellular proton and calcium concentrations in different cell classes were simultaneously imaged and the specific activation of T lymphocytes was demonstrated. This method should be especially useful for drug screening due to the small sample volume and high information density. (+info)Shrinkage-induced activation of Na+/H+ exchange in rat renal mesangial cells. (4/2851)
Using the pH-sensitive dye 2', 7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF), we examined the effect of hyperosmolar solutions, which presumably caused cell shrinkage, on intracellular pH (pHi) regulation in mesangial cells (single cells or populations) cultured from the rat kidney. The calibration of BCECF is identical in shrunken and unshrunken mesangial cells if the extracellular K+ concentration ([K+]) is adjusted to match the predicted intracellular [K+]. For pHi values between approximately 6.7 and approximately 7.4, the intrinsic buffering power in shrunken cells (600 mosmol/kgH2O) is threefold larger than in unshrunken cells (approximately 300 mosmol/kgH2O). In the nominal absence of CO2/HCO-3, exposing cell populations to a HEPES-buffered solution supplemented with approximately 300 mM mannitol (600 mosmol/kgH2O) causes steady-state pHi to increase by approximately 0.4. The pHi increase is due to activation of Na+/H+ exchange because, in single cells, it is blocked in the absence of external Na+ or in the presence of 50 microM ethylisopropylamiloride (EIPA). Preincubating cells in a Cl--free solution for at least 14 min inhibits the shrinkage-induced pHi increase by 80%. We calculated the pHi dependence of the Na+/H+ exchange rate in cell populations under normosmolar and hyperosmolar conditions by summing 1) the pHi dependence of the total acid-extrusion rate and 2) the pHi dependence of the EIPA-insensitive acid-loading rate. Shrinkage alkali shifts the pHi dependence of Na+/H+ exchange by approximately 0.7 pH units. (+info)Distribution of 5-chloromethylfluorescein diacetate staining during meiotic maturation and fertilization in vitro of mouse oocytes. (5/2851)
The aim of this confocal microscopy study was to determine whether the pattern of CellTracker Green 5-chloromethylfluorescein diacetate (CMFDA) staining changes during meiotic maturation and fertilization in vitro of mouse oocytes. At different times during meiotic maturation and fertilization, oocytes, zygotes and two-cell embryos were stained with CMFDA to demonstrate intracellular glutathione S-transferase activity. After washing in CMFDA-free medium, most oocytes, zygotes and embryos were stained with dihydroethidium (HE) to visualize DNA structures. Meiotic maturation and fertilization in vitro of mouse oocytes were associated with changes in the pattern of intracellular CMFDA staining. In particular, accumulations of CMFDA-positive membranes were observed around the nucleus of germinal vesicle (GV) oocytes, overlaying the sperm nucleus as well as overlaying the first mitotic spindle if this approached the plasma membrane. Staining of oocytes and zygotes with the probes 3,3'-dihexyloxacarbocyanine iodine [DiOC6(3)], which stains all the intracellular membranes, and rhodamine 123, which stains active mitochondria, demonstrated that the intracellular structures evidenced by CMFDA staining did not correspond to accumulations of mitochondria. Exposure of oocytes and zygotes to the microtubule-disrupting agent nocodazole or the actin-depolymerizing drug cytochalasin D revealed an autonomous microfilament-dependent transport and relocation of CMFDA-positive membranes during meiotic maturation and fertilization. Such a transport of CMFDA-positive membranes may be envisaged as a protective shield built to prevent damage to DNA from endogenous and exogenous mutagen metabolites. (+info)Partitioning of triphenylalkylphosphonium homologues in gel bead-immobilized liposomes: chromatographic measurement of their membrane partition coefficients. (6/2851)
Unilamellar liposomes of small or large size, SUVs and LUVs, respectively, were stably immobilized in the highly hydrophilic Sepharose 4B or Sephacryl S-1000 gel beads as a membrane stationary phase for immobilized liposome chromatography (ILC). Lipophilic cations of triphenylmethylphosphonium and tetraphenylphosphonium (TPP+) have been used as probes of the membrane potential of cells. Interaction of TPP+ and triphenylalkylphosphonium homologues with the immobilized liposomal membranes was shown by their elution profiles on both zonal and frontal ILC. Retardation of the lipophilic cations on the liposome gel bed was increased as the hydrophobicity of the cations increased, indicating the partitioning of lipophilic cations into the hydrocarbon region of the membranes. The cations did not retard on the Sepharose or Sephacryl gel bed without liposomes, confirming that the cations only interact with the immobilized liposomes. Effects of the solute concentration, flow rate, and gel-matrix substance on the ILC were studied. The stationary phase volume of the liposomal membranes was calculated from the volume of a phospholipid molecule and the amount of the immobilized phospholipid, which allowed us to determine the membrane partition coefficient (KLM) for the lipophilic cations distributed between the aqueous mobile and membrane stationary phases. The values of KLM were generally increased with the hydrophobicity of the solutes increased, and were higher for the SUVs than for the LUVs. The ILC method described here can be applied to measure membrane partition coefficients for other lipophilic solutes (e.g., drugs). (+info)Chemical transformations in individual ultrasmall biomimetic containers. (7/2851)
Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes. (+info)Kinetics of lactate and pyruvate transport in cultured rat myotubes. (8/2851)
Skeletal muscle transport of lactate and pyruvate was studied in primary cultures of rat myotubes, applying the pH-sensitive fluorescent indicator 2', 7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. The initial rate of decrease in intracellular pH (pHi) upon lactate or pyruvate incubation was used to determine total transport (carrier mediated and diffusion). Both lactate and pyruvate transport could be inhibited by a combination of 0.5 mM 4,4'-diisothiocyanostilbene-2, 2'-disulfonic acid, 5 mM mersalyl and 10 mM alpha-cyano-4-hydroxycinnamate. The kinetic parameters, Km and Vmax, for carrier-mediated transport of lactate were 9.9+/-1.1 mM and 0. 69+/-0.02 mmol l-1 s-1, respectively. For pyruvate, Km and Vmax were 4.4+/-1.3 mM and 0.30+/-0.05 mmol l-1 s-1, respectively. The diffusion component of the total transport was 0.0040+/-0.0005[S] (n=4) and 0.0048+/-0.0003[S] (n=4) for lactate and pyruvate, respectively. Furthermore, it was observed that the two monocarboxylate transporter isoforms present in mature skeletal muscles, MCT1 and MCT4 (formerly called MCT3 (M.C. Wilson, V.N. Jackson, C. Heddle, N.T. Price, H. Pilegaard, C. Juel, A. Bonen, I. Montgomery, O.F. Hutter, A.P. Halestrap, Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3, J. Biol. Chem. 273 (1998) 15920-15926)), were also expressed in primary culture of myotubes. (+info)
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Direct fluorescein-conjugated antibody synonyms, direct fluorescein-conjugated antibody antonyms - FreeThesaurus.com
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Cell sorting: automated separation of mammalian cells as a function of intracellular fluorescence. - PubMed - NCBI
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Carboxyfluorescein succinimidyl ester - Wikipedia
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JCI -
Resident lung antigen-presenting cells have the capacity to promote Th2 T cell differentiation in situ
Probing the nucleotide binding site of sarcoplasmic reticulum (Ca2+ -Mg2)-ATPase with anti-fluorescein antibodies | Biochemical...
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Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO
Photoactivatable fluorescein derivatives with azidomethyl caging groups for tracing oligonucleotides in living human cells
THE STUDY ON DETERMINATION OF β-GLUCAN BY FLUORIMETRY WITH SODIUM FLUORESCEIN--《Food Research and Development》2006年10期
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Effect of Nickel Chloride on Cell Proliferation
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Specific Staining of Ornithosis Virus by Fluorescein-labelled Incomplete Antibodies. - Semantic Scholar
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RCSB PDB
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RCSB PDB
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Electroporation affects the transfer of fluorescein thr | Open-i
What is Fluorescein? (with pictures)
Biomerica Announces Two New China CFDA Approvals and a New Distribution Channel in China
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Ophthalmic artery
Fluorescein angiography. References[edit]. .mw-parser-output .reflist{font-size:90%;margin-bottom:0.5em;list-style-type:decimal ...
60 Cycle
"Fluorescein , Biography & History , AllMusic". AllMusic. Retrieved 2016-09-04. "Troy Van Leeuwen , Queens of the Stone Age - ... Van Leeuwen and Scott went on to tour with the band Failure while Rubenstein joined the group Fluorescein. Both of these ...
Brassicaceae
... are almost exclusively pollinated by insects. A chemical mechanism in the pollen is active in many species to avoid selfing. Two notable exceptions are exclusive self pollination in closed flowers in Cardamine chenopodifolia, and wind pollination in Pringlea antiscorbutica.[7] Although it can be pollinated, Alliaria petiolata is self-fertile. Most species reproduce sexually through seed, but Cardamine bulbifera produces gemmae and in others, such as Cardamine pentaphyllos, the coral-like roots easily break into segments, that will grow into separate plants.[7] In some species, such as in the genus Cardamine, seed pods open with force and so catapult the seeds quite far. Many of these have sticky seed coats, assisting long distance dispersal by animals, and this may also explain several intercontinental dispersal events in the genus, and its near global distribution. Brassicaceae are common on serpentine and dolomite rich in magnesium. Over a hundred species in the family accumulate ...
Angiography
Fluorescein angiography is a medical procedure in which a fluorescent dye is injected into the bloodstream. The dye highlights ... "Fluorescein Angiography". EMPIRE RETINA CONSULTANTS. Retrieved 22 August 2016. Kashani AH, Chen CL, Gahm JK, Zheng F, Richter ... Cardiac catheterization Computed tomography angiography Contrast medium Echocardiogram Electrocardiogram Fluorescein ...
Heinz Falk
Fluorescein Conjugate. Biochim. Biophys. Acta, 1427, 33 - 43 (1999) C. Etzlstorfer, I Gutman and H. Falk, Concerning the ...
Biological effects of high-energy visible light
A particular study claimed a large difference in observed fluorescein angiography examinations concluding they observed ... Bennett, Timothy J. (2017). "Fluorescein Fundamentals". Ophthalmic Photographers' Society. M, Rosenfield; RT, Li; NT, Kirsch ( ...
Optical coherence tomography angiography
"Fluorescein Angiography". Alander, Jarmo T.; Kaartinen, Ilkka; Laakso, Aki; et al. (2012). "A Review of Indocyanine Green ... Gass, JDM; Sever, RJ; Sparks, D; Goren, J (1967). "A combined technique of fluorescein fundoscopy and angiography of the eye". ... 1986). "Fluorescein angiography complication survey". Ophthalmology. 93 (5): 611-7. doi:10.1016/s0161-6420(86)33697-2. PMID ... Spaide, Richard F.; Klancnik, James M.; Cooney, Michael J. (2015-01-01). "Retinal Vascular Layers Imaged by Fluorescein ...
Joey Rubenstein
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Uveitis
"Fluorescein eye stain". NIH. Archived from the original on 20 May 2012. Retrieved 15 May 2012. Ruggieri S, Frassanito MA, ...
Fluorescent glucose biosensor
Sato, K. and J. Anzai, Fluorometric determination of sugars using fluorescein-labeled concanavalin A-glycogen conjugates. Anal ... fluorescein (485, 520); NBD (490, 540); NBDE (490, 530); JPW4039 (485, 590); JPW4042 (470, 640); and JPW4045 (470, 640)). Out ...
DICOM - Wikipédia, a enciclopédia livre
FA = Fluorescein Angiography. *FS = Fundoscopy. *HC = Hard Copy. *LP = Laparoscopy. *LS = Laser Surface Scan ...
Blacklight
Manufacturers of ethylene glycol-containing antifreezes commonly add fluorescein, which causes the patient's urine to fluoresce ... "Urine fluorescence using a Wood's lamp to detect the antifreeze additive sodium fluorescein: a qualitative adjunctive test in ...
Pyranine
Fluorescein Fluorescence "chem industry entry". "Comparative Toxicogenomics Database entry". "chemical land 21 entry". "Sci- ...
Resorcinol
With the anhydrides of dibasic acids, it yields fluoresceins. When heated with calcium chloride-ammonia to 200 °C it yields ...
Bascom Palmer Eye Institute
J. Donald M. Gass, a macular degeneration specialist developed the use of fluorescein angiography as a diagnostic tool, and Dr ... CS1 maint: discouraged parameter (link) Norton, EW; Gutman, F (1965). "Diabetic retinopathy studied by fluorescein angiography ... pioneered the use of fluorescein angiography for the diagnosis of macular and retinal diseases, which led to the accurate ...
Oligonucleotide synthesis
Brush, C. K. "Fluorescein Labelled Phosphoramidites". (1996) U.S. Patent 5,583,236. Pitner, J. B.; Linn, C. P. "Synthesis and ... exemplified by 6-FAM amidite 7 for the attachment of fluorescein and dabcyl amidite 8, respectively), hydrophilic and ...
Arnall Patz
Patz, Arnall; Fine, Stuart L. (1977). Interpretation of the Fundus Fluorescein Angiogram. Boston: Little, Brown. OCLC 3206372. ...
Branch retinal vein occlusion
Fluorescein angiography is a helpful adjunct. Findings include delayed venous filling, hypofluorescence caused by hemorrhage ...
Oxygen radical absorbance capacity
Fluorescein is currently used most as a fluorescent probe. Equipment that can automatically measure and calculate the capacity ... Most of them employ the same principle (i.e. measurement of AAPH-radical mediated damage of fluorescein); however, ORAC-EPR, ... The degeneration (or decomposition) of fluorescein is measured as the presence of the antioxidant slows the fluorescence decay ... The assay measures the oxidative degradation of the fluorescent molecule (either beta-phycoerythrin or fluorescein) after being ...
Conjunctiva
Meyer, P. A. (1988-01-01). "Patterns of blood flow in episcleral vessels studied by low-dose fluorescein videoangiography". Eye ... Fluorescein angiography has been used to study the blood flow of the bulbar conjunctiva and to differentiate the bulbar ... "Low dose fluorescein angiography of the conjunctiva and episclera". British Journal of Ophthalmology. 71 (1): 2-10. doi ...
Susac's syndrome
Fluorescein angiography may demonstrate leakage in areas remote from the retinal infarctions. In a recent analysis (Susac et al ... Gass Plaques and Fluorescein Leakage in Susac Syndrome. Journal of Neurological Sciences 299(1-2): 97-100; 2010 Susac JO, ... Both patients underwent fluorescein retinal angiography that demonstrated multifocal retinal artery occlusions without evidence ...
6-Carboxyfluorescein
A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents ... Fluorescein Molecular Imaging Products Company (2005-08-26). "5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg ...
Foveal avascular zone
It is an important landmark in Fluorescein angiography. Its diameter is 0.5mm, the central 1.5 degrees of an individual's ...
Ventricular septal defect
j) Multiple muscular VSDs are a challenge to close, achieving a complete closure can be aided by the use of fluorescein dye. ... "Use of Fluorescein Dye to Identify Residual Defects". Ann Thorac Surg. 97 (1): e27-8. doi:10.1016/j.athoracsur.2013.10.059. ...
Corneal ulcer
The use of fluorescein stain, which is taken up by exposed corneal stroma and appears green, helps in defining the margins of ... Next, the cornea is stained with 1% fluorescein. The ulcer is stained green. Corneal ulcers are a common human eye disease. ...
Jeffrey W. Berger
Variability in fluorescein angiography interpretation for photodynamic therapy in age-related macular degeneration. Retina. ... Quantitative image sequence analysis of fundus fluorescein angiography. Ophthalmic Surg Lasers. 1999 Jan;30(1):72-3. 19. Shin ... Quantitative, spatio-temporal image analysis of fluorescein angiography in age-related macular degeneration. Proc SPIE 1998; ... of quantitative retinal imaging with a number of publications on image analysis methods for modalities such as fluorescein ...
K-casein
Κ-casein labeled with the fluorochrome fluorescein isothiocyanate (FITC) to yield the fluorescein thiocarbamoyl (FTC) ... casein labeled with the fluorochrome fluorescein isothiocyanate (FITC) to yield the fluorescein thiocarbamoyl (FTC) derivative ... Ageitos, J.M.; Vallejo, J.A.; Poza, M.; Villa, T.G. (2006). "Fluorescein Thiocarbamoyl-Kappa-Casein Assay for the Specific ... InterPro: IPR000117 Kappa casein Fluorescein Thiocarbamoyl-Kappa-Casein Assay for the Specific Testing of Milk-Clotting ...
Eosin
... Y is a tetrabromo derivative of fluorescein. Eosin B is a dibromo dinitro derivative of fluorescein. Eosin is most often ... and stains them dark red or pink as a result of the actions of bromine on fluorescein. In addition to staining proteins in the ...
Coarctation of the aorta
Fluorescein dye can aid in the localisation of chyle leak. Hypertension is defined when a patient's blood pressure in the arm ... "Intraoperative Identification of Chyle Leak During Coarctation Repair Using Fluorescein Dye". The Annals of Thoracic Surgery. ...
Tadpole pupil
Fluorescein Angiogram: evaluation of blood circulation in the retina. Dilated Pupillary Exam: special drops expand the pupil, ...
Fluorescein - Wikipedia
Main article: Fluorescein (medical use). Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a ... Fluorescein is also known as a color additive (D&C Yellow no. 7). The disodium salt form of fluorescein is known as uranine or ... Wikimedia Commons has media related to Fluorescein.. *Absorption and Emission Spectra of Fluorescein in Ethanol and Basic ... Many derivatives of fluorescein are known. Example *fluorescein isothiocyanate 1, often abbreviated as FITC, features an ...
FLUORESCEIN ANGIOGRAPHY
... (FFA). This is a special test used for examination of blood vessels in the eye. It is an office ... Fluorescein Angiography may be done when any retinal disease, especially that involving the blood vessels is suspected, like ... A bright yellow- green dye, called fluorescein is injected into a vein in the arm. Various photos of the blood vessels are ...
fluorescein (lactone form) (CHEBI:31624)
... has functional parent fluorescein (acid form) (CHEBI:172923) fluorescein (lactone form ... fluorescein (lactone form) (CHEBI:31624) is a γ-lactone (CHEBI:37581) fluorescein (lactone form) (CHEBI:31624) is a 2- ... fluorescein (lactone form) (CHEBI:31624) is a oxaspiro compound (CHEBI:37948) fluorescein (lactone form) (CHEBI:31624) is a ... fluorescein isothiocyanate (CHEBI:37926) has functional parent fluorescein (lactone form) (CHEBI:31624). magnesium green (CHEBI ...
Fluorescein angiography: MedlinePlus Medical Encyclopedia
Fluorescein angiography is an eye test that uses a special dye and camera to look at blood flow in the retina and choroid. ... Fluorescein angiography is an eye test that uses a special dye and camera to look at blood flow in the retina and choroid. ... After the first group of pictures is taken, a dye called fluorescein is injected into a vein. Most often it is injected at the ... Retinal fluorescein angiography: a sensitive and specific tool to predict coronary slow flow. Egypt Heart J. 2018;70(3):167-171 ...
Fluorescein - Everything2.com
Fluorescein is used in medicine to diagnose corneal ulcers and certain other eye disorders. A solution of the chemical is ... fluorescein isothiocyanate is a fluorescent chemical derived from fluorescein which is used to label proteins. fluorescein- ... Fluorescein diacetate. fluorescence immunoassay. fluorescent label. Clone High. Ponce de Leon. Aquaplaning. grid reference. ... Fluorescein is used in medicine to diagnose corneal ulcers and certain other eye disorders. A solution of the chemical is ...
Minims fluorescein sodium
Minims fluorescein are single-use preservative-free eye drops that contain the active ingredient fluorescein. This is a ... Minims fluorescein are single-use preservative-free eye drops that contain the active ingredient fluorescein. This is a ... Fluorescein can also be used to find foreign bodies such as grit in the eye, as they show up surrounded by a green ring. ... Fluorescein can only enter damaged cells of the eye. It will temporarily stain any cells it enters and therefore marks any ...
Fluorescein amidite - Wikipedia
Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide ... Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons ... FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the ... US 5583236, Brush, C. K., "Fluorescein Labelled Phosphoramidites" ...
Fluorescein eye stain: MedlinePlus Medical Encyclopedia
Fluorescein diacetate - Everything2.com
One use is in determining the viability of pollen grains; grains which are alive take up the fluorescein diacet... ... One use is in determining the viability of pollen grains; grains which are alive take up the fluorescein diacetate and ... hydrolyze it intracellularly; the released fluorescein will fluoresce, and can be detected with a microscope using filters that ...
Fluorescein - definition of fluorescein by The Free Dictionary
fluorescein synonyms, fluorescein pronunciation, fluorescein translation, English dictionary definition of fluorescein. n. An ... Related to fluorescein: fluorescein isothiocyanate, Fluorescein sodium. fluo·res·ce·in. (flo͝o-rĕs′ē-ĭn, flô-, flō-). n.. An ... fluorescein. (ˌflʊəˈrɛsɪɪn) or fluoresceine. n. (Elements & Compounds) an orange-red crystalline compound that in aqueous ... Fluorescein staining is enhanced with cell degeneration or cell death, which increases membrane permeability to fluorescein.. ...
Fluoresceine - definition of fluoresceine by The Free Dictionary
fluoresceine synonyms, fluoresceine pronunciation, fluoresceine translation, English dictionary definition of fluoresceine. ... Noun 1. fluoresceine - a yellow dye that is visible even when highly diluted; used as an absorption indicator when silver ... Related to fluoresceine: fluorescein isothiocyanate, Fluorescein sodium, fluorescein strips. ThesaurusAntonymsRelated Words ... Fluoresceine - definition of fluoresceine by The Free Dictionary https://www.thefreedictionary.com/fluoresceine ...
Fluorescein angiogram - American Academy of Ophthalmology
Fluorescein | Memorial Sloan Kettering Cancer Center
CLIP-Cell™ Fluorescein | NEB
... is based on dipivaloylfluorescein and is suitable for standard fluorescein filter sets ... Fluorescein is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells or in ... Figure 3. Structure of CLIP-Cell Fluorescein (MW 756.8 g/mol).. Product Categories:. Discontinued Products. * Properties & ... CLIP-Cell™ Fluorescein is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells ...
FLUORESCITE (fluorescein sodium injection, solution) | Healthgrades.com
Fluorescein dye disappearance - American Academy of Ophthalmology
What is Fluorescein? (with pictures)
Fluorescein is a fluorescent dye thats used in everything from eye exams to oceanography. Many researchers use fluorescein to ... Several chemical companies manufacture fluorescein and derivatives of this compound, such as fluorescein isothiocyanate. These ... Is fluorescein detection possible with the naked eye, or do the molecules only glow in the dark or under something like a dark ... In pure state, fluorescein is a red or orange powder which is dissolved in alkaline solutions to make up a yellow solution ...
Fluorescite (Fluorescein): Uses, Dosage, Side Effects, Interactions, Warning
Fluorescein) may treat, side effects, dosage, drug interactions, warnings, patient labeling, reviews, and related medications ... FLUORESCITE® (fluorescein injection, USP) 10% contains fluorescein sodium (equivalent to fluorescein 10% w/v). It is a sterile ... Fluorescein undergoes rapid metabolism to fluorescein monoglucuronide. After IV administration of fluorescein sodium (14 mg/kg ... Single use 5 mL vial containing 100 mg/mL fluorescein.. Storage And Handling. FLUORESCITE® (fluorescein injection, USP) 10% is ...
CTTv Allelic Ladder Mix, (Fluorescein), 150ul
To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...
Anti-HA-Fluorescein Troubleshooting | Sigma-Aldrich
Fluorescein / proparacaine ophthalmic and tafenoquine Drug Interactions - Drugs.com
RCSB PDB - 1DZH: P14-FLUORESCEIN-N135Q-S380C-ANTITHROMBIN-III
Fluorescein (Intravenous Route) Description and Brand Names - Mayo Clinic
Fluorescein - DrugBank
Fluorescein Lite. Fluorescein sodium (100 mg/1mL). Injection, solution. Intravenous. Altaire Pharmaceuticals Inc.. 2001-10-01. ... Fluorescein Lite. Fluorescein sodium (250 mg/1mL). Injection, solution. Intravenous. Altaire Pharmaceuticals Inc.. 2002-01-01. ... Minims Lidocaine and Fluorescein. Fluorescein sodium (0.25 %) + Lidocaine hydrochloride (4 %). Solution / drops. Ophthalmic; ... The excretion of Fluorescein can be decreased when combined with Acetaminophen.. Acetazolamide. The excretion of Fluorescein ...
fluorescein isothiocyanate dextran | China-Mainland | Sigma-Aldrich
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.1,2,3 Conflicting data on the branch lengths implies that the average branch length is less than three glucose units.4,5 However, other methods indicate branches of greater than 50 glucose units exist.6,7 Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.8,9,10 Lower MW dextrans will exhibit slightly less branching4 and have a more narrow range of MW distribution.11 Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry.7,12,13 Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil.12 At MW below 2,000, dextran is more rod-like.14 The MW of dextran is measured by one or more of the following methods: low angle laser light scattering,15 size exclusion chromatography,16 ...
Anti-Fluorescein antibody (ab19491) | Abcam
Rabbit polyclonal Fluorescein antibody validated for WB, ELISA. Referenced in 8 publications. Immunogen corresponding to ... Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to ... Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and ... Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 ...
Ak-Fluor (Fluorescein Injection): Side Effects, Interactions, Warning, Dosage & Uses
Fluorescein Injection) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and ... AK-FLUOR® (Fluorescein Injection, USP) is a sterile solution in Water for Injection, of Fluorescein prepared with the aid of ... Fluorescein has been demonstrated to be excreted in human milk. Caution should be exercised when AK-FLUOR®(Fluorescein ... AK-FLUOR®, 10% (Fluorescein Injection, USP - Sterile) 100 mg/mL. NDC 17478-253-10 5 mL single dose vials in packs of 12. NDC ...
Streptavidin, Fluorescein conjugated/tagged from R&D Systems
Fluorescein conjugated/tagged from R&D Systems,Request Info,biological,biology supply,biology supplies,biology product ... ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene. 8. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent ... CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON. 11. ApopTag Fluorescein Direct In Situ Apoptosis Detection ... Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio ...