Fluoresceins. Medical search

A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.

A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.

Visualization of a vascular system after intravenous injection of a fluorescein solution. The images may be photographed or televised. It is used especially in studying the retinal and uveal vasculature.

Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Measurement of light given off by fluorescein in order to assess the integrity of various ocular barriers. The method is used to investigate the blood-aqueous barrier, blood-retinal barrier, aqueous flow measurements, corneal endothelial permeability, and tear flow dynamics.

The concave interior of the eye, consisting of the retina, the choroid, the sclera, the optic disk, and blood vessels, seen by means of the ophthalmoscope. (Cline et al., Dictionary of Visual Science, 4th ed)

Organic derivatives of thiocyanic acid which contain the general formula R-SCN.

Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.

The blood vessels which supply and drain the RETINA.

A pathological process consisting of the formation of new blood vessels in the CHOROID.

The thin, highly vascular membrane covering most of the posterior of the eye between the RETINA and SCLERA.

The fluid secreted by the lacrimal glands. This fluid moistens the CONJUNCTIVA and CORNEA.

A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.

Corneal and conjunctival dryness due to deficient tear production, predominantly in menopausal and post-menopausal women. Filamentary keratitis or erosion of the conjunctival and corneal epithelium may be caused by these disorders. Sensation of the presence of a foreign body in the eye and burning of the eyes may occur.

The transparent, semigelatinous substance that fills the cavity behind the CRYSTALLINE LENS of the EYE and in front of the RETINA. It is contained in a thin hyaloid membrane and forms about four fifths of the optic globe.

A tricarbocyanine dye that is used diagnostically in liver function tests and to determine blood volume and cardiac output.

The use of green light-producing LASERS to stop bleeding. The green light is selectively absorbed by HEMOGLOBIN, thus triggering BLOOD COAGULATION.

Clarity or sharpness of OCULAR VISION or the ability of the eye to see fine details. Visual acuity depends on the functions of RETINA, neuronal transmission, and the interpretative ability of the brain. Normal visual acuity is expressed as 20/20 indicating that one can see at 20 feet what should normally be seen at that distance. Visual acuity can also be influenced by brightness, color, and contrast.

A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.

Measurement of the intensity and quality of fluorescence.

A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.

The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.

Disorders of the choroid including hereditary choroidal diseases, neoplasms, and other abnormalities of the vascular layer of the uvea.

Degenerative changes in the RETINA usually of older adults which results in a loss of vision in the center of the visual field (the MACULA LUTEA) because of damage to the retina. It occurs in dry and wet forms.

Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.

Examination of the interior of the eye with an ophthalmoscope.

An oval area in the retina, 3 to 5 mm in diameter, usually located temporal to the posterior pole of the eye and slightly below the level of the optic disk. It is characterized by the presence of a yellow pigment diffusely permeating the inner layers, contains the fovea centralis in its center, and provides the best phototropic visual acuity. It is devoid of retinal blood vessels, except in its periphery, and receives nourishment from the choriocapillaris of the choroid. (From Cline et al., Dictionary of Visual Science, 4th ed)

An analytical method for detecting and measuring FLUORESCENCE in compounds or targets such as cells, proteins, or nucleotides, or targets previously labeled with FLUORESCENCE AGENTS.

An imaging method using LASERS that is used for mapping subsurface structure. When a reflective site in the sample is at the same optical path length (coherence) as the reference mirror, the detector observes interference fringes.

The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)

Sterile solutions that are intended for instillation into the eye. It does not include solutions for cleaning eyeglasses or CONTACT LENS SOLUTIONS.

A specialized transport barrier, in the EYE, formed by the retinal pigment EPITHELIUM, and the ENDOTHELIUM of the BLOOD VESSELS of the RETINA. TIGHT JUNCTIONS joining adjacent cells keep the barrier between cells continuous.

A tetraiodofluorescein used as a red coloring in some foods (cherries, fish), as a disclosure of DENTAL PLAQUE, and as a stain of some cell types. It has structural similarity to THYROXINE.

Fluid accumulation in the outer layer of the MACULA LUTEA that results from intraocular or systemic insults. It may develop in a diffuse pattern where the macula appears thickened or it may acquire the characteristic petaloid appearance referred to as cystoid macular edema. Although macular edema may be associated with various underlying conditions, it is most commonly seen following intraocular surgery, venous occlusive disease, DIABETIC RETINOPATHY, and posterior segment inflammatory disease. (From Survey of Ophthalmology 2004; 49(5) 470-90)

Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.

A red fluorescein dye used as a histologic stain. It may be cytotoxic, mutagenic, and inhibit certain mitochondrial functions.

Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.

The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.

Central retinal artery and its branches. It arises from the ophthalmic artery, pierces the optic nerve and runs through its center, enters the eye through the porus opticus and branches to supply the retina.

Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.

The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.

Blockage of the RETINAL VEIN. Those at high risk for this condition include patients with HYPERTENSION; DIABETES MELLITUS; ATHEROSCLEROSIS; and other CARDIOVASCULAR DISEASES.

The space in the eye, filled with aqueous humor, bounded anteriorly by the cornea and a small portion of the sclera and posteriorly by a small portion of the ciliary body, the iris, and that part of the crystalline lens which presents through the pupil. (Cline et al., Dictionary of Visual Science, 4th ed, p109)

Diseases of the uvea.

The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

The photography of images produced on a fluorescent screen by X-rays.

Method of making images on a sensitized surface by exposure to light or other radiant energy.

A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.

The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.

Central retinal vein and its tributaries. It runs a short course within the optic nerve and then leaves and empties into the superior ophthalmic vein or cavernous sinus.

Sudden ISCHEMIA in the RETINA due to blocked blood flow through the CENTRAL RETINAL ARTERY or its branches leading to sudden complete or partial loss of vision, respectively, in the eye.

Bleeding from the vessels of the retina.

The coagulation of tissue by an intense beam of light, including laser (LASER COAGULATION). In the eye it is used in the treatment of retinal detachments, retinal holes, aneurysms, hemorrhages, and malignant and benign neoplasms. (Dictionary of Visual Science, 3d ed)

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

Organic compounds which contain mercury as an integral part of the molecule.

Formation of new blood vessels originating from the retinal veins and extending along the inner (vitreal) surface of the retina.

An area approximately 1.5 millimeters in diameter within the macula lutea where the retina thins out greatly because of the oblique shifting of all layers except the pigment epithelium layer. It includes the sloping walls of the fovea (clivus) and contains a few rods in its periphery. In its center (foveola) are the cones most adapted to yield high visual acuity, each cone being connected to only one ganglion cell. (Cline et al., Dictionary of Visual Science, 4th ed)

The white, opaque, fibrous, outer tunic of the eyeball, covering it entirely excepting the segment covered anteriorly by the cornea. It is essentially avascular but contains apertures for vessels, lymphatics, and nerves. It receives the tendons of insertion of the extraocular muscles and at the corneoscleral junction contains the canal of Schlemm. (From Cline et al., Dictionary of Visual Science, 4th ed)

The administration of substances into the VITREOUS BODY of the eye with a hypodermic syringe.

The clear, watery fluid which fills the anterior and posterior chambers of the eye. It has a refractive index lower than the crystalline lens, which it surrounds, and is involved in the metabolism of the cornea and the crystalline lens. (Cline et al., Dictionary of Visual Science, 4th ed, p319)

Separation of the inner layers of the retina (neural retina) from the pigment epithelium. Retinal detachment occurs more commonly in men than in women, in eyes with degenerative myopia, in aging and in aphakia. It may occur after an uncomplicated cataract extraction, but it is seen more often if vitreous humor has been lost during surgery. (Dorland, 27th ed; Newell, Ophthalmology: Principles and Concepts, 7th ed, p310-12).

An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.

The most anterior portion of the uveal layer, separating the anterior chamber from the posterior. It consists of two layers - the stroma and the pigmented epithelium. Color of the iris depends on the amount of melanin in the stroma on reflection from the pigmented epithelium.

Green dyes containing ammonium and aryl sulfonate moieties that facilitate the visualization of tissues, if given intravenously. They have mostly been used in the study of kidney physiology.

The mucous membrane that covers the posterior surface of the eyelids and the anterior pericorneal surface of the eyeball.

The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye.

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

The rate dynamics in chemical or physical systems.

Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.

Therapy using oral or topical photosensitizing agents with subsequent exposure to light.

Swelling of the OPTIC DISK, usually in association with increased intracranial pressure, characterized by hyperemia, blurring of the disk margins, microhemorrhages, blind spot enlargement, and engorgement of retinal veins. Chronic papilledema may cause OPTIC ATROPHY and visual loss. (Miller et al., Clinical Neuro-Ophthalmology, 4th ed, p175)

Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.

The administration of substances into the eye with a hypodermic syringe.

Recording of electric potentials in the retina after stimulation by light.

Introduction of substances into the body using a needle and syringe.

The selectively permeable barrier, in the EYE, formed by the nonpigmented layer of the EPITHELIUM of the CILIARY BODY, and the ENDOTHELIUM of the BLOOD VESSELS of the IRIS. TIGHT JUNCTIONS joining adjacent cells keep the barrier between cells continuous.

A visual impairment characterized by the accumulation of fluid under the retina through a defect in the retinal pigment epithelium.

Inflammation of the choroid.

The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

Devices for examining the interior of the eye, permitting the clear visualization of the structures of the eye at any depth. (UMDNS, 1999)

Drying and inflammation of the conjunctiva as a result of insufficient lacrimal secretion. When found in association with XEROSTOMIA and polyarthritis, it is called SJOGREN'S SYNDROME.

Inflammation of the retinal vasculature with various causes including infectious disease; LUPUS ERYTHEMATOSUS, SYSTEMIC; MULTIPLE SCLEROSIS; BEHCET SYNDROME; and CHORIORETINITIS.

The prototypical uricosuric agent. It inhibits the renal excretion of organic anions and reduces tubular reabsorption of urate. Probenecid has also been used to treat patients with renal impairment, and, because it reduces the renal tubular excretion of other drugs, has been used as an adjunct to antibacterial therapy.

Microscopy in which the image is formed by ultraviolet radiation and is displayed and recorded by means of photographic film.

Small breaks in the elastin-filled tissue of the retina.

The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.

Discharge of cerebrospinal fluid through the nose. Common etiologies include trauma, neoplasms, and prior surgery, although the condition may occur spontaneously. (Otolaryngol Head Neck Surg 1997 Apr;116(4):442-9)

The escape of diagnostic or therapeutic material from the vessel into which it is introduced into the surrounding tissue or body cavity.

The minute vessels that connect the arterioles and venules.

The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT.

Measurement of the various properties of light.

The sebaceous glands situated on the inner surface of the eyelids between the tarsal plates and CONJUNCTIVA.

A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.

Elements of limited time intervals, contributing to particular results or situations.

Permanent dilation of preexisting blood vessels (CAPILLARIES; ARTERIOLES; VENULES) creating small focal red lesions, most commonly in the skin or mucous membranes. It is characterized by the prominence of skin blood vessels, such as vascular spiders.

Diseases affecting the eye.

Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.

Colloid or hyaline bodies lying beneath the retinal pigment epithelium. They may occur either secondary to changes in the choroid that affect the pigment epithelium or as an autosomal dominant disorder of the retinal pigment epithelium.

The metal-free red phycobilin pigment in a conjugated chromoprotein of red algae. It functions as a light-absorbing substance together with chlorophylls.

Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue. These compounds can be administered topically or systemically and have been used therapeutically to treat psoriasis and various types of neoplasms.

The single layer of pigment-containing epithelial cells in the RETINA, situated closely to the tips (outer segments) of the RETINAL PHOTORECEPTOR CELLS. These epithelial cells are macroglia that perform essential functions for the photoreceptor cells, such as in nutrient transport, phagocytosis of the shed photoreceptor membranes, and ensuring retinal attachment.

The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.

Tumors of the choroid; most common intraocular tumors are malignant melanomas of the choroid. These usually occur after puberty and increase in incidence with advancing age. Most malignant melanomas of the uveal tract develop from benign melanomas (nevi).

Inflammation of the choroid in which the sensory retina becomes edematous and opaque. The inflammatory cells and exudate may burst through the sensory retina to cloud the vitreous body.

Exudates are fluids, CELLS, or other cellular substances that are slowly discharged from BLOOD VESSELS usually from inflamed tissues. Transudates are fluids that pass through a membrane or squeeze through tissue or into the EXTRACELLULAR SPACE of TISSUES. Transudates are thin and watery and contain few cells or PROTEINS.

The application of drug preparations to the surfaces of the body, especially the skin (ADMINISTRATION, CUTANEOUS) or mucous membranes. This method of treatment is used to avoid systemic side effects when high doses are required at a localized area or as an alternative systemic administration route, to avoid hepatic processing for example.

Single layer of large flattened cells covering the surface of the cornea.

Diseases, dysfunctions, or disorders of or located in the iris.

The tear-forming and tear-conducting system which includes the lacrimal glands, eyelid margins, conjunctival sac, and the tear drainage system.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Small antigenic determinants capable of eliciting an immune response only when coupled to a carrier. Haptens bind to antibodies but by themselves cannot elicit an antibody response.

A mixture of alkylbenzyldimethylammonium compounds. It is a bactericidal quaternary ammonium detergent used topically in medicaments, deodorants, mouthwashes, as a surgical antiseptic, and as a as preservative and emulsifier in drugs and cosmetics.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Antibodies produced by a single clone of cells.

Damage or trauma inflicted to the eye by external means. The concept includes both surface injuries and intraocular injuries.

The bystander effect in the HSVtk/ganciclovir system and its relationship to gap junctional communication. (1/2851)

The bystander effect (BSE) is an interesting and important property of the herpes thymidine kinase/ganciclovir (hTK/GCV) system of gene therapy for cancer. With the BSE, not only are the hTK expressing cells killed upon ganciclovir (GCV) exposure but also neighboring wild-type tumor cells. On testing a large number of tumor cell lines in vitro, a wide range of sensitivity to bystander killing was found. Since transfer of toxic GCV metabolites from hTK-modified to wild-type tumor cells via gap junctions (GJ) seemed to be a likely mechanism of the BSE, we tested GJ function in these various tumors with a dye transfer technique and pharmacological agents known to affect GJ communication. We confirmed that mixtures of tumor cell resistant to the BSE did not show dye transfer from cell to cell while bystander-sensitive tumor cells did. Dieldrin, a drug known to decrease GJ communication, diminished dye transfer and also inhibited the BSE. Forskolin, an upregulator of cAMP did increase GJ, but directly inhibited hTK and therefore its effect on BSE could not be determined. We conclude that these observations further support port the concept that functional GJ play an important role in the BSE and further suggest that pharmacological manipulation of GJ may influence the outcome of cancer therapy with hTK/GCV. (+info)

Overexpression of the multidrug resistance-associated protein (MRP1) in human heavy metal-selected tumor cells. (2/2851)

Cellular and molecular mechanisms involved in the resistance to cytotoxic heavy metals remain largely to be characterized in mammalian cells. To this end, we have analyzed a metal-resistant variant of the human lung cancer GLC4 cell line that we have selected by a step-wise procedure in potassium antimony tartrate. Antimony-selected cells, termed GLC4/Sb30 cells, poorly accumulated antimony through an enhanced cellular efflux of metal, thus suggesting up-regulation of a membrane export system in these cells. Indeed, GLC4/Sb30 cells were found to display a functional overexpression of the multidrug resistance-associated protein MRP1, a drug export pump, as demonstrated by Western blotting, reverse transcriptase-polymerase chain reaction and calcein accumulation assays. Moreover, MK571, a potent inhibitor of MRP1 activity, was found to markedly down-modulate resistance of GLC4/Sb30 cells to antimony and to decrease cellular export of the metal. Taken together, our data support the conclusion that overexpression of functional MRP1 likely represents one major mechanism by which human cells can escape the cytotoxic effects of heavy metals. (+info)

Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers. (3/2851)

A fluorimetric multi-parameter cell sensor at the single cell level is presented which makes it possible to observe the physiological behavior of different cell lines, different physiological parameters, and statistical data at the same time. Different cell types were immobilized at predefined positions with high accuracy using optical tweezers and adhesion promoting surface layers. The process is applicable to both adherent and non-adherent cells. Coating of the immobilization area with mussel adhesive protein was shown to be essential for the process. Intracellular proton and calcium concentrations in different cell classes were simultaneously imaged and the specific activation of T lymphocytes was demonstrated. This method should be especially useful for drug screening due to the small sample volume and high information density. (+info)

Shrinkage-induced activation of Na+/H+ exchange in rat renal mesangial cells. (4/2851)

Using the pH-sensitive dye 2', 7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF), we examined the effect of hyperosmolar solutions, which presumably caused cell shrinkage, on intracellular pH (pHi) regulation in mesangial cells (single cells or populations) cultured from the rat kidney. The calibration of BCECF is identical in shrunken and unshrunken mesangial cells if the extracellular K+ concentration ([K+]) is adjusted to match the predicted intracellular [K+]. For pHi values between approximately 6.7 and approximately 7.4, the intrinsic buffering power in shrunken cells (600 mosmol/kgH2O) is threefold larger than in unshrunken cells (approximately 300 mosmol/kgH2O). In the nominal absence of CO2/HCO-3, exposing cell populations to a HEPES-buffered solution supplemented with approximately 300 mM mannitol (600 mosmol/kgH2O) causes steady-state pHi to increase by approximately 0.4. The pHi increase is due to activation of Na+/H+ exchange because, in single cells, it is blocked in the absence of external Na+ or in the presence of 50 microM ethylisopropylamiloride (EIPA). Preincubating cells in a Cl--free solution for at least 14 min inhibits the shrinkage-induced pHi increase by 80%. We calculated the pHi dependence of the Na+/H+ exchange rate in cell populations under normosmolar and hyperosmolar conditions by summing 1) the pHi dependence of the total acid-extrusion rate and 2) the pHi dependence of the EIPA-insensitive acid-loading rate. Shrinkage alkali shifts the pHi dependence of Na+/H+ exchange by approximately 0.7 pH units. (+info)

Distribution of 5-chloromethylfluorescein diacetate staining during meiotic maturation and fertilization in vitro of mouse oocytes. (5/2851)

The aim of this confocal microscopy study was to determine whether the pattern of CellTracker Green 5-chloromethylfluorescein diacetate (CMFDA) staining changes during meiotic maturation and fertilization in vitro of mouse oocytes. At different times during meiotic maturation and fertilization, oocytes, zygotes and two-cell embryos were stained with CMFDA to demonstrate intracellular glutathione S-transferase activity. After washing in CMFDA-free medium, most oocytes, zygotes and embryos were stained with dihydroethidium (HE) to visualize DNA structures. Meiotic maturation and fertilization in vitro of mouse oocytes were associated with changes in the pattern of intracellular CMFDA staining. In particular, accumulations of CMFDA-positive membranes were observed around the nucleus of germinal vesicle (GV) oocytes, overlaying the sperm nucleus as well as overlaying the first mitotic spindle if this approached the plasma membrane. Staining of oocytes and zygotes with the probes 3,3'-dihexyloxacarbocyanine iodine [DiOC6(3)], which stains all the intracellular membranes, and rhodamine 123, which stains active mitochondria, demonstrated that the intracellular structures evidenced by CMFDA staining did not correspond to accumulations of mitochondria. Exposure of oocytes and zygotes to the microtubule-disrupting agent nocodazole or the actin-depolymerizing drug cytochalasin D revealed an autonomous microfilament-dependent transport and relocation of CMFDA-positive membranes during meiotic maturation and fertilization. Such a transport of CMFDA-positive membranes may be envisaged as a protective shield built to prevent damage to DNA from endogenous and exogenous mutagen metabolites. (+info)

Partitioning of triphenylalkylphosphonium homologues in gel bead-immobilized liposomes: chromatographic measurement of their membrane partition coefficients. (6/2851)

Unilamellar liposomes of small or large size, SUVs and LUVs, respectively, were stably immobilized in the highly hydrophilic Sepharose 4B or Sephacryl S-1000 gel beads as a membrane stationary phase for immobilized liposome chromatography (ILC). Lipophilic cations of triphenylmethylphosphonium and tetraphenylphosphonium (TPP+) have been used as probes of the membrane potential of cells. Interaction of TPP+ and triphenylalkylphosphonium homologues with the immobilized liposomal membranes was shown by their elution profiles on both zonal and frontal ILC. Retardation of the lipophilic cations on the liposome gel bed was increased as the hydrophobicity of the cations increased, indicating the partitioning of lipophilic cations into the hydrocarbon region of the membranes. The cations did not retard on the Sepharose or Sephacryl gel bed without liposomes, confirming that the cations only interact with the immobilized liposomes. Effects of the solute concentration, flow rate, and gel-matrix substance on the ILC were studied. The stationary phase volume of the liposomal membranes was calculated from the volume of a phospholipid molecule and the amount of the immobilized phospholipid, which allowed us to determine the membrane partition coefficient (KLM) for the lipophilic cations distributed between the aqueous mobile and membrane stationary phases. The values of KLM were generally increased with the hydrophobicity of the solutes increased, and were higher for the SUVs than for the LUVs. The ILC method described here can be applied to measure membrane partition coefficients for other lipophilic solutes (e.g., drugs). (+info)

Chemical transformations in individual ultrasmall biomimetic containers. (7/2851)

Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes. (+info)

Kinetics of lactate and pyruvate transport in cultured rat myotubes. (8/2851)

Skeletal muscle transport of lactate and pyruvate was studied in primary cultures of rat myotubes, applying the pH-sensitive fluorescent indicator 2', 7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. The initial rate of decrease in intracellular pH (pHi) upon lactate or pyruvate incubation was used to determine total transport (carrier mediated and diffusion). Both lactate and pyruvate transport could be inhibited by a combination of 0.5 mM 4,4'-diisothiocyanostilbene-2, 2'-disulfonic acid, 5 mM mersalyl and 10 mM alpha-cyano-4-hydroxycinnamate. The kinetic parameters, Km and Vmax, for carrier-mediated transport of lactate were 9.9+/-1.1 mM and 0. 69+/-0.02 mmol l-1 s-1, respectively. For pyruvate, Km and Vmax were 4.4+/-1.3 mM and 0.30+/-0.05 mmol l-1 s-1, respectively. The diffusion component of the total transport was 0.0040+/-0.0005[S] (n=4) and 0.0048+/-0.0003[S] (n=4) for lactate and pyruvate, respectively. Furthermore, it was observed that the two monocarboxylate transporter isoforms present in mature skeletal muscles, MCT1 and MCT4 (formerly called MCT3 (M.C. Wilson, V.N. Jackson, C. Heddle, N.T. Price, H. Pilegaard, C. Juel, A. Bonen, I. Montgomery, O.F. Hutter, A.P. Halestrap, Lactic acid efflux from white skeletal muscle is catalyzed by the monocarboxylate transporter isoform MCT3, J. Biol. Chem. 273 (1998) 15920-15926)), were also expressed in primary culture of myotubes. (+info)

Fluorescein angiography. References[edit]. .mw-parser-output .reflist{font-size:90%;margin-bottom:0.5em;list-style-type:decimal ...

https://en.wikipedia.org/wiki/Ophthalmic_artery

"Fluorescein , Biography & History , AllMusic". AllMusic. Retrieved 2016-09-04. "Troy Van Leeuwen , Queens of the Stone Age - ... Van Leeuwen and Scott went on to tour with the band Failure while Rubenstein joined the group Fluorescein. Both of these ...

https://en.wikipedia.org/wiki/60_Cycle

... are almost exclusively pollinated by insects. A chemical mechanism in the pollen is active in many species to avoid selfing. Two notable exceptions are exclusive self pollination in closed flowers in Cardamine chenopodifolia, and wind pollination in Pringlea antiscorbutica.[7] Although it can be pollinated, Alliaria petiolata is self-fertile. Most species reproduce sexually through seed, but Cardamine bulbifera produces gemmae and in others, such as Cardamine pentaphyllos, the coral-like roots easily break into segments, that will grow into separate plants.[7] In some species, such as in the genus Cardamine, seed pods open with force and so catapult the seeds quite far. Many of these have sticky seed coats, assisting long distance dispersal by animals, and this may also explain several intercontinental dispersal events in the genus, and its near global distribution. Brassicaceae are common on serpentine and dolomite rich in magnesium. Over a hundred species in the family accumulate ...

https://en.wikipedia.org/wiki/Brassicaceae

Fluorescein angiography is a medical procedure in which a fluorescent dye is injected into the bloodstream. The dye highlights ... "Fluorescein Angiography". EMPIRE RETINA CONSULTANTS. Retrieved 22 August 2016. Kashani AH, Chen CL, Gahm JK, Zheng F, Richter ... Cardiac catheterization Computed tomography angiography Contrast medium Echocardiogram Electrocardiogram Fluorescein ...

https://en.wikipedia.org/wiki/Angiography

Fluorescein Conjugate. Biochim. Biophys. Acta, 1427, 33 - 43 (1999) C. Etzlstorfer, I Gutman and H. Falk, Concerning the ...

https://en.wikipedia.org/wiki/Heinz_Falk

A particular study claimed a large difference in observed fluorescein angiography examinations concluding they observed ... Bennett, Timothy J. (2017). "Fluorescein Fundamentals". Ophthalmic Photographers' Society. M, Rosenfield; RT, Li; NT, Kirsch ( ...

https://en.wikipedia.org/wiki/Biological_effects_of_high-energy_visible_light

"Fluorescein Angiography". Alander, Jarmo T.; Kaartinen, Ilkka; Laakso, Aki; et al. (2012). "A Review of Indocyanine Green ... Gass, JDM; Sever, RJ; Sparks, D; Goren, J (1967). "A combined technique of fluorescein fundoscopy and angiography of the eye". ... 1986). "Fluorescein angiography complication survey". Ophthalmology. 93 (5): 611-7. doi:10.1016/s0161-6420(86)33697-2. PMID ... Spaide, Richard F.; Klancnik, James M.; Cooney, Michael J. (2015-01-01). "Retinal Vascular Layers Imaged by Fluorescein ...

https://en.wikipedia.org/wiki/Optical_coherence_tomography_angiography

"Artist/Fluorescein". billboard.com. "Fluorescein Cathy's on Crank!". allmusic.com. "Weezer Poster". wolfgangs.com. "Finally, ... "Fluorescein Revels in the Dark Side". latimes.com. "Bar Crawling From Boutique to Boutique". nytimes.com. "The Final Frontier ...

https://en.wikipedia.org/wiki/Joey_Rubenstein

"Fluorescein eye stain". NIH. Archived from the original on 20 May 2012. Retrieved 15 May 2012. Ruggieri S, Frassanito MA, ...

https://en.wikipedia.org/wiki/Uveitis

Sato, K. and J. Anzai, Fluorometric determination of sugars using fluorescein-labeled concanavalin A-glycogen conjugates. Anal ... fluorescein (485, 520); NBD (490, 540); NBDE (490, 530); JPW4039 (485, 590); JPW4042 (470, 640); and JPW4045 (470, 640)). Out ...

https://en.wikipedia.org/wiki/Fluorescent_glucose_biosensor

FA = Fluorescein Angiography. *FS = Fundoscopy. *HC = Hard Copy. *LP = Laparoscopy. *LS = Laser Surface Scan ...

https://pt.wikipedia.org/wiki/DICOM

Manufacturers of ethylene glycol-containing antifreezes commonly add fluorescein, which causes the patient's urine to fluoresce ... "Urine fluorescence using a Wood's lamp to detect the antifreeze additive sodium fluorescein: a qualitative adjunctive test in ...

https://en.wikipedia.org/wiki/Black_light

Fluorescein Fluorescence "chem industry entry". "Comparative Toxicogenomics Database entry". "chemical land 21 entry". "Sci- ...

https://en.wikipedia.org/wiki/Pyranine

With the anhydrides of dibasic acids, it yields fluoresceins. When heated with calcium chloride-ammonia to 200 °C it yields ...

https://en.wikipedia.org/wiki/Resorcinol

J. Donald M. Gass, a macular degeneration specialist developed the use of fluorescein angiography as a diagnostic tool, and Dr ... CS1 maint: discouraged parameter (link) Norton, EW; Gutman, F (1965). "Diabetic retinopathy studied by fluorescein angiography ... pioneered the use of fluorescein angiography for the diagnosis of macular and retinal diseases, which led to the accurate ...

https://en.wikipedia.org/wiki/Bascom_Palmer_Eye_Institute

Brush, C. K. "Fluorescein Labelled Phosphoramidites". (1996) U.S. Patent 5,583,236. Pitner, J. B.; Linn, C. P. "Synthesis and ... exemplified by 6-FAM amidite 7 for the attachment of fluorescein and dabcyl amidite 8, respectively), hydrophilic and ...

https://en.wikipedia.org/wiki/Oligonucleotide_synthesis

Patz, Arnall; Fine, Stuart L. (1977). Interpretation of the Fundus Fluorescein Angiogram. Boston: Little, Brown. OCLC 3206372. ...

https://en.wikipedia.org/wiki/Arnall_Patz

Fluorescein angiography is a helpful adjunct. Findings include delayed venous filling, hypofluorescence caused by hemorrhage ...

https://en.wikipedia.org/wiki/Branch_retinal_vein_occlusion

Fluorescein is currently used most as a fluorescent probe. Equipment that can automatically measure and calculate the capacity ... Most of them employ the same principle (i.e. measurement of AAPH-radical mediated damage of fluorescein); however, ORAC-EPR, ... The degeneration (or decomposition) of fluorescein is measured as the presence of the antioxidant slows the fluorescence decay ... The assay measures the oxidative degradation of the fluorescent molecule (either beta-phycoerythrin or fluorescein) after being ...

https://en.wikipedia.org/wiki/Oxygen_radical_absorbance_capacity

Meyer, P. A. (1988-01-01). "Patterns of blood flow in episcleral vessels studied by low-dose fluorescein videoangiography". Eye ... Fluorescein angiography has been used to study the blood flow of the bulbar conjunctiva and to differentiate the bulbar ... "Low dose fluorescein angiography of the conjunctiva and episclera". British Journal of Ophthalmology. 71 (1): 2-10. doi ...

https://en.wikipedia.org/wiki/Conjunctiva

Fluorescein angiography may demonstrate leakage in areas remote from the retinal infarctions. In a recent analysis (Susac et al ... Gass Plaques and Fluorescein Leakage in Susac Syndrome. Journal of Neurological Sciences 299(1-2): 97-100; 2010 Susac JO, ... Both patients underwent fluorescein retinal angiography that demonstrated multifocal retinal artery occlusions without evidence ...

https://en.wikipedia.org/wiki/Susac's_syndrome

A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents ... Fluorescein Molecular Imaging Products Company (2005-08-26). "5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg ...

https://en.wikipedia.org/wiki/6-Carboxyfluorescein

It is an important landmark in Fluorescein angiography. Its diameter is 0.5mm, the central 1.5 degrees of an individual's ...

https://en.wikipedia.org/wiki/Foveal_avascular_zone

j) Multiple muscular VSDs are a challenge to close, achieving a complete closure can be aided by the use of fluorescein dye. ... "Use of Fluorescein Dye to Identify Residual Defects". Ann Thorac Surg. 97 (1): e27-8. doi:10.1016/j.athoracsur.2013.10.059. ...

https://en.wikipedia.org/wiki/Ventricular_septal_defect

The use of fluorescein stain, which is taken up by exposed corneal stroma and appears green, helps in defining the margins of ... Next, the cornea is stained with 1% fluorescein. The ulcer is stained green. Corneal ulcers are a common human eye disease. ...

https://en.wikipedia.org/wiki/Corneal_ulcer

Variability in fluorescein angiography interpretation for photodynamic therapy in age-related macular degeneration. Retina. ... Quantitative image sequence analysis of fundus fluorescein angiography. Ophthalmic Surg Lasers. 1999 Jan;30(1):72-3. 19. Shin ... Quantitative, spatio-temporal image analysis of fluorescein angiography in age-related macular degeneration. Proc SPIE 1998; ... of quantitative retinal imaging with a number of publications on image analysis methods for modalities such as fluorescein ...

https://en.wikipedia.org/wiki/Jeffrey_W._Berger

Κ-casein labeled with the fluorochrome fluorescein isothiocyanate (FITC) to yield the fluorescein thiocarbamoyl (FTC) ... casein labeled with the fluorochrome fluorescein isothiocyanate (FITC) to yield the fluorescein thiocarbamoyl (FTC) derivative ... Ageitos, J.M.; Vallejo, J.A.; Poza, M.; Villa, T.G. (2006). "Fluorescein Thiocarbamoyl-Kappa-Casein Assay for the Specific ... InterPro: IPR000117 Kappa casein Fluorescein Thiocarbamoyl-Kappa-Casein Assay for the Specific Testing of Milk-Clotting ...

https://en.wikipedia.org/wiki/K-casein

... Y is a tetrabromo derivative of fluorescein. Eosin B is a dibromo dinitro derivative of fluorescein. Eosin is most often ... and stains them dark red or pink as a result of the actions of bromine on fluorescein. In addition to staining proteins in the ...

https://en.wikipedia.org/wiki/Eosin

Fluorescein dye can aid in the localisation of chyle leak. Hypertension is defined when a patient's blood pressure in the arm ... "Intraoperative Identification of Chyle Leak During Coarctation Repair Using Fluorescein Dye". The Annals of Thoracic Surgery. ...

https://en.wikipedia.org/wiki/Coarctation_of_the_aorta

Fluorescein Angiogram: evaluation of blood circulation in the retina. Dilated Pupillary Exam: special drops expand the pupil, ...

https://en.wikipedia.org/wiki/Tadpole_pupil

Main article: Fluorescein (medical use). Fluorescein sodium, the sodium salt of fluorescein, is used extensively as a ... Fluorescein is also known as a color additive (D&C Yellow no. 7). The disodium salt form of fluorescein is known as uranine or ... Wikimedia Commons has media related to Fluorescein.. *Absorption and Emission Spectra of Fluorescein in Ethanol and Basic ... Many derivatives of fluorescein are known. Example *fluorescein isothiocyanate 1, often abbreviated as FITC, features an ...

https://en.wikipedia.org/wiki/Fluorescein

... (FFA). This is a special test used for examination of blood vessels in the eye. It is an office ... Fluorescein Angiography may be done when any retinal disease, especially that involving the blood vessels is suspected, like ... A bright yellow- green dye, called fluorescein is injected into a vein in the arm. Various photos of the blood vessels are ...

http://www.members.tripod.com/manisha_b/Eye_Exams/Fluorescein_Angiography.htm

... has functional parent fluorescein (acid form) (CHEBI:172923) fluorescein (lactone form ... fluorescein (lactone form) (CHEBI:31624) is a γ-lactone (CHEBI:37581) fluorescein (lactone form) (CHEBI:31624) is a 2- ... fluorescein (lactone form) (CHEBI:31624) is a oxaspiro compound (CHEBI:37948) fluorescein (lactone form) (CHEBI:31624) is a ... fluorescein isothiocyanate (CHEBI:37926) has functional parent fluorescein (lactone form) (CHEBI:31624). magnesium green (CHEBI ...

https://www.ebi.ac.uk/chebi/searchId.do?chebiId=31624

Fluorescein angiography is an eye test that uses a special dye and camera to look at blood flow in the retina and choroid. ... Fluorescein angiography is an eye test that uses a special dye and camera to look at blood flow in the retina and choroid. ... After the first group of pictures is taken, a dye called fluorescein is injected into a vein. Most often it is injected at the ... Retinal fluorescein angiography: a sensitive and specific tool to predict coronary slow flow. Egypt Heart J. 2018;70(3):167-171 ...

https://medlineplus.gov/ency/article/003846.htm

Fluorescein is used in medicine to diagnose corneal ulcers and certain other eye disorders. A solution of the chemical is ... fluorescein isothiocyanate is a fluorescent chemical derived from fluorescein which is used to label proteins. fluorescein- ... Fluorescein diacetate. fluorescence immunoassay. fluorescent label. Clone High. Ponce de Leon. Aquaplaning. grid reference. ... Fluorescein is used in medicine to diagnose corneal ulcers and certain other eye disorders. A solution of the chemical is ...

https://everything2.com/title/Fluorescein

Minims fluorescein are single-use preservative-free eye drops that contain the active ingredient fluorescein. This is a ... Minims fluorescein are single-use preservative-free eye drops that contain the active ingredient fluorescein. This is a ... Fluorescein can also be used to find foreign bodies such as grit in the eye, as they show up surrounded by a green ring. ... Fluorescein can only enter damaged cells of the eye. It will temporarily stain any cells it enters and therefore marks any ...

http://www.netdoctor.co.uk/medicines/eyes/a7112/minims-fluorescein-sodium/

Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide ... Oligonucleotides labeled with fluorescein at one of the termini and with a quencher at the other can serve as molecular beacons ... FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the ... US 5583236, Brush, C. K., "Fluorescein Labelled Phosphoramidites" ...

https://en.wikipedia.org/wiki/Fluorescein_amidite

... fluorescein) and a blue light to detect foreign bodies in the eye. This test can also detect damage to the cornea. The cornea ... This is a test that uses orange dye (fluorescein) and a blue light to detect foreign bodies in the eye. This test can also ...

https://medlineplus.gov/ency/article/003845.htm

One use is in determining the viability of pollen grains; grains which are alive take up the fluorescein diacet... ... One use is in determining the viability of pollen grains; grains which are alive take up the fluorescein diacetate and ... hydrolyze it intracellularly; the released fluorescein will fluoresce, and can be detected with a microscope using filters that ...

https://everything2.com/title/Fluorescein+diacetate

fluorescein synonyms, fluorescein pronunciation, fluorescein translation, English dictionary definition of fluorescein. n. An ... Related to fluorescein: fluorescein isothiocyanate, Fluorescein sodium. fluo·res·ce·in. (flo͝o-rĕs′ē-ĭn, flô-, flō-). n.. An ... fluorescein. (ˌflʊəˈrɛsɪɪn) or fluoresceine. n. (Elements & Compounds) an orange-red crystalline compound that in aqueous ... Fluorescein staining is enhanced with cell degeneration or cell death, which increases membrane permeability to fluorescein.. ...

https://www.thefreedictionary.com/fluorescein

fluoresceine synonyms, fluoresceine pronunciation, fluoresceine translation, English dictionary definition of fluoresceine. ... Noun 1. fluoresceine - a yellow dye that is visible even when highly diluted; used as an absorption indicator when silver ... Related to fluoresceine: fluorescein isothiocyanate, Fluorescein sodium, fluorescein strips. ThesaurusAntonymsRelated Words ... Fluoresceine - definition of fluoresceine by The Free Dictionary https://www.thefreedictionary.com/fluoresceine ...

https://www.thefreedictionary.com/fluoresceine

Fluorescein angiogram of the left eye. a. This image shows an arterial phase of the dye, with areas of hypofluorescence ... Fluorescein angiogram of the left eye. a. This image shows an arterial phase of the dye, with areas of hypofluorescence ...

https://www.aao.org/image/fluorescein-angiogram-4

https://www.mskcc.org/cancer-care/patient-education/fluorescein-01

... is based on dipivaloylfluorescein and is suitable for standard fluorescein filter sets ... Fluorescein is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells or in ... Figure 3. Structure of CLIP-Cell Fluorescein (MW 756.8 g/mol).. Product Categories:. Discontinued Products. * Properties & ... CLIP-Cell™ Fluorescein is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells ...

https://www.neb.com/products/s9218-clip-cell-fluorescein

... fluorescein sodium injection, solution), including warnings and precautions, directions, and the names of other drugs and ...

https://www.healthgrades.com/drugs/fluorescite

Easiest and most common, place fluorescein drops in each eye, Wait five minutes, Objectively grade remaining fluorescein. If it ...

https://www.aao.org/image/fluorescein-dye-disappearance

Fluorescein is a fluorescent dye thats used in everything from eye exams to oceanography. Many researchers use fluorescein to ... Several chemical companies manufacture fluorescein and derivatives of this compound, such as fluorescein isothiocyanate. These ... Is fluorescein detection possible with the naked eye, or do the molecules only glow in the dark or under something like a dark ... In pure state, fluorescein is a red or orange powder which is dissolved in alkaline solutions to make up a yellow solution ...

http://www.wisegeek.com/what-is-fluorescein.htm

Fluorescein) may treat, side effects, dosage, drug interactions, warnings, patient labeling, reviews, and related medications ... FLUORESCITE® (fluorescein injection, USP) 10% contains fluorescein sodium (equivalent to fluorescein 10% w/v). It is a sterile ... Fluorescein undergoes rapid metabolism to fluorescein monoglucuronide. After IV administration of fluorescein sodium (14 mg/kg ... Single use 5 mL vial containing 100 mg/mL fluorescein.. Storage And Handling. FLUORESCITE® (fluorescein injection, USP) 10% is ...

https://www.rxlist.com/fluorescite-drug.htm

To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...

https://www.promega.com/resources/msds/msdss/dg2000/dg2121/

2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. Reproduction of any materials from the site is strictly forbidden without permission. Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. Site Use Terms , Privacy ...

https://www.sigmaaldrich.com/technical-documents/articles/biology/roche/anti-ha-fluorescein.html

A Moderate Drug Interaction exists between fluorescein / proparacaine ophthalmic and tafenoquine. View detailed information ... Drug Interactions between fluorescein / proparacaine ophthalmic and tafenoquine. This report displays the potential drug ...

https://www.drugs.com/drug-interactions/fluorescein-proparacaine-ophthalmic-with-tafenoquine-1109-0-3928-0.html

We determined the crystal structure of an activated antithrombin variant, N135Q S380C-fluorescein (P14-fluorescein), in order ... A 2. 85-A Structure of a Fluorescein Derivative Reveals an Electrostatic Link between the Hinge and Heparin Binding Regions.. ...

https://www.rcsb.org/structure/1DZH

Fluorescein injection is used to help certain parts of the eye (eg, retina, iris) become more visible during eye medical ...

https://www.mayoclinic.org/drugs-supplements/fluorescein-intravenous-route/description/drg-20311323

Fluorescein Lite. Fluorescein sodium (100 mg/1mL). Injection, solution. Intravenous. Altaire Pharmaceuticals Inc.. 2001-10-01. ... Fluorescein Lite. Fluorescein sodium (250 mg/1mL). Injection, solution. Intravenous. Altaire Pharmaceuticals Inc.. 2002-01-01. ... Minims Lidocaine and Fluorescein. Fluorescein sodium (0.25 %) + Lidocaine hydrochloride (4 %). Solution / drops. Ophthalmic; ... The excretion of Fluorescein can be decreased when combined with Acetaminophen.. Acetazolamide. The excretion of Fluorescein ...

https://www.drugbank.ca/drugs/DB00693

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.1,2,3 Conflicting data on the branch lengths implies that the average branch length is less than three glucose units.4,5 However, other methods indicate branches of greater than 50 glucose units exist.6,7 Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million.8,9,10 Lower MW dextrans will exhibit slightly less branching4 and have a more narrow range of MW distribution.11 Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry.7,12,13 Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil.12 At MW below 2,000, dextran is more rod-like.14 The MW of dextran is measured by one or more of the following methods: low angle laser light scattering,15 size exclusion chromatography,16 ...

https://www.sigmaaldrich.com/china-mainland/zh/technical-documents/protocols/biology/fluorescein-isothiocyanate-dextran.html

Rabbit polyclonal Fluorescein antibody validated for WB, ELISA. Referenced in 8 publications. Immunogen corresponding to ... Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to ... Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and ... Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 ...

https://www.abcam.com/fluorescein-antibody-ab19491.html

Fluorescein Injection) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and ... AK-FLUOR® (Fluorescein Injection, USP) is a sterile solution in Water for Injection, of Fluorescein prepared with the aid of ... Fluorescein has been demonstrated to be excreted in human milk. Caution should be exercised when AK-FLUOR®(Fluorescein ... AK-FLUOR®, 10% (Fluorescein Injection, USP - Sterile) 100 mg/mL. NDC 17478-253-10 5 mL single dose vials in packs of 12. NDC ...

https://www.rxlist.com/ak-fluor-drug.htm

Fluorescein conjugated/tagged from R&D Systems,Request Info,biological,biology supply,biology supplies,biology product ... ABsolute QRT-PCR SYBR Green Fluorescein Mix from ABgene. 8. Label IT siRNA Tracker Fluorescein Kit with TransIT-siQUEST Reagent ... CaspaTag™ Pan-Caspase In Situ Assay Kit, Fluorescein from CHEMICON. 11. ApopTag Fluorescein Direct In Situ Apoptosis Detection ... Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio ...

http://www.bio-medicine.org/biology-products/Streptavidin--Fluorescein-conjugated-tagged-from-R-26D-Systems-4707-1/

Shop a large selection of Tertiary Colors Stains Dyes and Indicators products and learn more about Fluorescein, disodium salt, ...

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