Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.
The flow of BLOOD through or around an organ or region of the body.
A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.
A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A cell line derived from cultured tumor cells.
The degree of replication of the chromosome set in the karyotype.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Rhythmic, intermittent propagation of a fluid through a BLOOD VESSEL or piping system, in contrast to constant, smooth propagation, which produces laminar flow.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
The change in gene frequency in a population due to migration of gametes or individuals (ANIMAL MIGRATION) across population barriers. In contrast, in GENETIC DRIFT the cause of gene frequency changes are not a result of population or gamete movement.
Antibodies produced by a single clone of cells.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
Elements of limited time intervals, contributing to particular results or situations.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
Established cell cultures that have the potential to propagate indefinitely.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The number of LYMPHOCYTES per unit volume of BLOOD.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
A classification of lymphocytes based on structurally or functionally different populations of cells.
DNA present in neoplastic tissue.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A subfamily of the family MURIDAE comprised of 69 genera. New World mice and rats are included in this subfamily.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
The circulation of blood through the BLOOD VESSELS of the BRAIN.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
Glycoproteins found on the membrane or surface of cells.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.
The circulation of blood through the CORONARY VESSELS of the HEART.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Adherence of cells to surfaces or to other cells.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
The metal-free red phycobilin pigment in a conjugated chromoprotein of red algae. It functions as a light-absorbing substance together with chlorophylls.
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Remnant of a tumor or cancer after primary, potentially curative therapy. (Dr. Daniel Masys, written communication)
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Specific molecular sites on the surface of various cells, including B-lymphocytes and macrophages, that combine with IMMUNOGLOBULIN Gs. Three subclasses exist: Fc gamma RI (the CD64 antigen, a low affinity receptor), Fc gamma RII (the CD32 antigen, a high affinity receptor), and Fc gamma RIII (the CD16 antigen, a low affinity receptor).
The continuous visual field seen by a subject through space and time.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
An encapsulated lymphatic organ through which venous blood filters.
A fluorescent probe with low toxicity which is a potent substrate for P-glycoprotein and the bacterial multidrug efflux transporter. It is used to assess mitochondrial bioenergetics in living cells and to measure the efflux activity of P-glycoprotein in both normal and malignant cells. (Leukemia 1997;11(7):1124-30)
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)
Methods for maintaining or growing CELLS in vitro.
A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.
Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.
A method of non-invasive, continuous measurement of MICROCIRCULATION. The technique is based on the values of the DOPPLER EFFECT of low-power laser light scattered randomly by static structures and moving tissue particulates.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Progenitor cells from which all blood cells derive.
55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.
A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.
A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.
A low affinity interleukin-2 receptor subunit that combines with the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN to form a high affinity receptor for INTERLEUKIN-2.
Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
The movement of the BLOOD as it is pumped through the CARDIOVASCULAR SYSTEM.
Measurement of the maximum rate of airflow attained during a FORCED VITAL CAPACITY determination. Common abbreviations are PEFR and PFR.
Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.
Assaying the products of or monitoring various biochemical processes and reactions in an individual cell.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
Migration of a foreign body from its original location to some other location in the body.
Surgery performed on the male genitalia.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.
Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.
Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
Experimentally induced neoplasms of CONNECTIVE TISSUE in animals to provide a model for studying human SARCOMA.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
Proteins prepared by recombinant DNA technology.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form.
The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.
Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.
A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Methods used to study CELLS.
Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.
The voltage difference, normally maintained at approximately -180mV, across the INNER MITOCHONDRIAL MEMBRANE, by a net movement of positive charge across the membrane. It is a major component of the PROTON MOTIVE FORCE in MITOCHONDRIA used to drive the synthesis of ATP.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
The artificial alteration of the fetal position to facilitate birth.
A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.
The rate dynamics in chemical or physical systems.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.
An adhesion-promoting leukocyte surface membrane heterodimer. The alpha subunit consists of the CD11b ANTIGEN and the beta subunit the CD18 ANTIGEN. The antigen, which is an integrin, functions both as a receptor for complement 3 and in cell-cell and cell-substrate adhesive interactions.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Investigative and diagnostic methods and procedures based on the photoacoustic effect, which is the generation of SOUND WAVES from the absorption of ELECTROMAGNETIC RADIATION.
Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.
The quantity of volume or surface area of CELLS.
Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.
The chromosomal constitution of a cell containing multiples of the normal number of CHROMOSOMES; includes triploidy (symbol: 3N), tetraploidy (symbol: 4N), etc.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
The force that opposes the flow of BLOOD through a vascular bed. It is equal to the difference in BLOOD PRESSURE across the vascular bed divided by the CARDIAC OUTPUT.
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
A subclass of IMIDES with the general structure of pyrrolidinedione. They are prepared by the distillation of ammonium succinate. They are sweet-tasting compounds that are used as chemical intermediates and plant growth stimulants.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A subclass of winged helix DNA-binding proteins that share homology with their founding member fork head protein, Drosophila.
A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein.
A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.
A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.
The action of a drug in promoting or enhancing the effectiveness of another drug.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
Agents obtained from higher plants that have demonstrable cytostatic or antineoplastic activity.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
The termination of the cell's ability to carry out vital functions such as metabolism, growth, reproduction, responsiveness, and adaptability.
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.

Interleukin-8 receptor modulates IgE production and B-cell expansion and trafficking in allergen-induced pulmonary inflammation. (1/36125)

We examined the role of the interleukin-8 (IL-8) receptor in a murine model of allergen-induced pulmonary inflammation using mice with a targeted deletion of the murine IL-8 receptor homologue (IL-8r-/-). Wild-type (Wt) and IL-8r-/- mice were systemically immunized to ovalbumin (OVA) and were exposed with either single or multiple challenge of aerosolized phosphate-buffered saline (OVA/PBS) or OVA (OVA/OVA). Analysis of cells recovered from bronchoalveolar lavage (BAL) revealed a diminished recruitment of neutrophils to the airway lumen after single challenge in IL-8r-/- mice compared with Wt mice, whereas multiply challenged IL-8r-/- mice had increased B cells and fewer neutrophils compared with Wt mice. Both Wt and IL-8r-/- OVA/OVA mice recruited similar numbers of eosinophils to the BAL fluid and exhibited comparable degrees of pulmonary inflammation histologically. Both total and OVA-specific IgE levels were greater in multiply challenged IL-8r-/- OVA/OVA mice than in Wt mice. Both the IL-8r-/- OVA/OVA and OVA/PBS mice were significantly less responsive to methacholine than their respective Wt groups, but both Wt and IL-8r mice showed similar degrees of enhancement after multiple allergen challenge. The data demonstrate that the IL-8r modulates IgE production, airway responsiveness, and the composition of the cells (B cells and neutrophils) recruited to the airway lumen in response to antigen.  (+info)

Cystic fibrosis transmembrane conductance regulator-mediated corneal epithelial cell ingestion of Pseudomonas aeruginosa is a key component in the pathogenesis of experimental murine keratitis. (2/36125)

Previous findings indicate that the cystic fibrosis transmembrane conductance regulator (CFTR) is a ligand for Pseudomonas aeruginosa ingestion into respiratory epithelial cells. In experimental murine keratitis, P. aeruginosa enters corneal epithelial cells. We determined the importance of CFTR-mediated uptake of P. aeruginosa by corneal cells in experimental eye infections. Entry of noncytotoxic (exoU) P. aeruginosa into human and rabbit corneal cell cultures was inhibited with monoclonal antibodies and peptides specific to CFTR amino acids 108 to 117. Immunofluorescence microscopy and flow cytometry demonstrated CFTR in the intact murine corneal epithelium, and electron microscopy showed that CFTR binds to P. aeruginosa following corneal cell ingestion. In experimental murine eye infections, multiple additions of 5 nM CFTR peptide 103-117 to inocula of either cytotoxic (exoU+) or noncytotoxic P. aeruginosa resulted in large reductions in bacteria in the eye and markedly lessened eye pathology. Compared with wild-type C57BL/6 mice, heterozygous DeltaF508 Cftr mice infected with P. aeruginosa had an approximately 10-fold reduction in bacterial levels in the eye and consequent reductions in eye pathology. Homozygous DeltaF508 Cftr mice were nearly completely resistant to P. aeruginosa corneal infection. CFTR-mediated internalization of P. aeruginosa by buried corneal epithelial cells is critical to the pathogenesis of experimental eye infection, while in the lung, P. aeruginosa uptake by surface epithelial cells enhances P. aeruginosa clearance from this tissue.  (+info)

Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. (3/36125)

Hermansky-Pudlak syndrome (HPS) is a genetic disorder characterized by defective lysosome-related organelles. Here, we report the identification of two HPS patients with mutations in the beta 3A subunit of the heterotetrameric AP-3 complex. The patients' fibroblasts exhibit drastically reduced levels of AP-3 due to enhanced degradation of mutant beta 3A. The AP-3 deficiency results in increased surface expression of the lysosomal membrane proteins CD63, lamp-1, and lamp-2, but not of nonlysosomal proteins. These differential effects are consistent with the preferential interaction of the AP-3 mu 3A subunit with tyrosine-based signals involved in lysosomal targeting. Our results suggest that AP-3 functions in protein sorting to lysosomes and provide an example of a human disease in which altered trafficking of integral membrane proteins is due to mutations in a component of the sorting machinery.  (+info)

Enhanced myocardial glucose use in patients with a deficiency in long-chain fatty acid transport (CD36 deficiency). (4/36125)

CD36 is a multifunctional, 88 kDa glycoprotein that is expressed on platelets and monocytes/macrophages. CD36 also has high homology with the long-chain fatty acid (LFA) transporter in the myocardium. Although platelet and monocyte CD36 levels can indicate a CD36 deficiency, they cannot predict specific clinical manifestations in the myocardium of a given person. We examined the hypothesis that a deficiency in LFA transport augments myocardial glucose uptake in patients with a type I CD36 deficiency. METHODS: Seven fasting patients with a type I CD36 deficiency and 9 controls were assessed by cardiac radionuclide imaging using beta-methyl-p-iodophenyl-pentadecanoic acid (BMIPP) as a LFA tracer and by PET with 18F-fluorodeoxyglucose (FDG). RESULTS: None of the patients with a CD36 deficiency showed myocardial uptake of BMIPP. The percentage dose uptake of BMIPP in these subjects was significantly lower than that in normal controls (1.31+/-0.24 versus 2.90+/-0.2; P < 0.005). PET studies revealed that myocardial FDG accumulation was substantially increased in patients with a CD36 deficiency. Quantitative analysis showed that the percentage dose uptake of FDG in patients with a CD36 deficiency was significantly higher than that in normal controls (1.28+/-0.35 versus 0.43+/-0.22; P< 0.01). CONCLUSION: CD36 functions as a major myocardial LFA transporter and its absence may cause a compensatory upregulation of myocardial glucose uptake.  (+info)

Proliferation and differentiation of rat theca-interstitial cells: comparison of effects induced by platelet-derived growth factor and insulin-like growth factor-I. (5/36125)

This study was designed to evaluate mechanisms regulating proliferation of steroidogenically active and steroidogenically inactive theca-interstitial (T-I) cells, and, specifically, to evaluate the effects of platelet-derived growth factor (PDGF) and insulin-like growth factor-I (IGF-I). T-I cells obtained from immature Sprague-Dawley rats were cultured in chemically defined media. Proliferation was assayed by thymidine incorporation and cell counting. Steroidogenically active cells were identified by the presence of 3beta-hydroxysteroid dehydrogenase activity. Flow cytometry facilitated separation of dividing cells (in S and G2/M phases of the cell cycle) from nondividing cells (in G0 and G1 phases of the cell cycle). PDGF alone (0.1-1 nM) produced a dose-dependent increase in DNA synthesis by up to 136%. IGF-I alone (10 nM) increased DNA synthesis by 56%. In the presence of both IGF-I (10 nM) and PDGF (0.1-1 nM), DNA synthesis increased by 108-214%. PDGF (1 nM) increased the total number of T-I cells by 43%; this effect was due to an increase in the number of steroidogenically inactive cells (47%). In contrast, the stimulatory effect of IGF-I (10 nM) was predominantly due to an increase in the number of steroidogenically active cells (163%). Separation of dividing cells from nondividing cells was accomplished with the aid of flow cytometry. In the absence of growth factors, the proportion of steroidogenically active cells was 35% lower among proliferating than resting cells. PDGF (1 nM) decreased the proportion of steroidogenically active cells among both proliferating and resting cells (by 43% and 16%, respectively). In contrast, IGF-I (10 nM) increased the proportion of steroidogenically active cells among proliferating cells by 56%. These findings indicate that differentiated/steroidogenically active cells divide; furthermore, PDGF and IGF-I may selectively stimulate proliferation of individual subpopulations of T-I cells, thereby providing a mechanism for development of structural and steroidogenically active components of the T-I compartment.  (+info)

Use of RhD fusion protein expressed on K562 cell surface in the study of molecular basis for D antigenic epitopes. (6/36125)

The human D antigens, one of the most clinically important blood groups, are presented by RhD protein with a putative 12 transmembrane topology. To understand the molecular basis for the complex antigenic profile of RhD protein, we expressed a series of RhD fusion proteins using different portions of Duffy protein as a tag in erythroleukemic K562 cells. Because the reactivity of monoclonal anti-RhD antibody, LOR15C9, depends mainly on the sequence coded by exon 7 of RhD, we altered DNA sequence corresponding to the amino acid residues 323-331(A) and 350-354(B) in the exon 7. The mutation in region B resulted in a severe reduction in LOR15C9 binding by flow cytometry analysis, suggesting that region B may play an important role in constituting antigen epitopes recognized by LOR15C9. On the other hand, a slight decrease in the antibody binding was observed for the region A mutant, suggesting that the intracellularly located region A may elicit a long distance effect on the formation of exofacial antigen epitopes. In addition, using various monoclonal antibodies against RhD, we compared the antigenic profile of expressed RhD fusion protein with that of endogenous RhD in K562 cells as well as in erythrocytes.  (+info)

Phenotypic and functional characterisation of myofibroblasts, macrophages, and lymphocytes migrating out of the human gastric lamina propria following the loss of epithelial cells. (7/36125)

BACKGROUND: The basement membrane of human colonic mucosa contains numerous discrete pores. We have recently shown that following loss of the surface epithelium, many cells migrate out of the colonic lamina propria via basement membrane pores. AIMS: To characterise cells migrating out via basement membrane pores of the human gastric lamina propria, following loss of the surface epithelium. METHODS: Fresh human gastric mucosal samples were completely denuded of epithelial cells and placed in culture. Tissue samples were studied by electron microscopy (EM) and cells by EM, FACS analysis, immunohistochemistry, and reverse transcription polymerase chain reaction (RT-PCR). RESULTS: EM showed numerous discrete pores (0. 65-8.29 microm in diameter) in the subepithelial basement membrane. During culture of mucosal samples denuded of epithelial cells, lymphocytes, macrophages, and myofibroblasts migrated out of the lamina propria via the basement membrane pores. The lymphocytes were predominantly CD45RO+ and CD69+ T cells. Macrophages were shown to express cyclooxygenase (COX) 1 and 2 enzymes. Myofibroblasts were established in culture and, despite prolonged culture and passage, retained their phenotype. They expressed mRNA and protein for COX 1 and 2 enzymes and their release of prostaglandin E2 was inhibited by selective COX 1 and 2 inhibitors. CONCLUSIONS: Lamina propria cells migrating out of cultured denuded gastric mucosal samples have been characterised phenotypically and functionally. Such cells would be suitable for studies of their interactions with epithelial cells and also with Helicobacter pylori and its products.  (+info)

Adenovirus mediated p53 tumour suppressor gene therapy for human gastric cancer cells in vitro and in vivo. (8/36125)

BACKGROUND/AIMS: Gastric cancer is one of the most prevalent forms of cancer in East Asia. Point mutation of the p53 gene has been reported in more than 60% of cases of gastric cancer and can lead to genetic instability and uncontrolled cell proliferation. The purpose of this investigation was to evaluate the potential of p53 gene therapy for gastric cancer. METHODS: The responses of human gastric cancer cell lines, MKN1, MKN7, MKN28, MKN45, and TMK-1, to recombinant adenoviruses encoding wild type p53 (AdCAp53) were analysed in vitro. The efficacy of the AdCAp53 treatment for MKN1 and MKN45 subcutaneous tumours in nude mice was assessed in vivo. RESULTS: p53-specific growth inhibition was observed in vitro in two of four gastric cancer cell lines with mutated p53, but not in the wild type p53 cell line. The mechanism of the killing of gastric cancer cells by AdCAp53 was found, by flow cytometric analysis and detection of DNA fragmentation, to be apoptosis. In vivo studies showed that the growth of subcutaneous tumours of p53 mutant MKN1 cells was significantly inhibited by direct injection of AdCAp53, but no significant growth inhibition was detected in the growth of p53 wild type MKN45 tumours. CONCLUSIONS: Adenovirus mediated reintroduction of wild type p53 is a potential clinical utility in gene therapy for gastric cancers.  (+info)

Computational tools will be employed for the analysis of multiparametric flow cytometry data. Classical two-dimensional scatter plots analysis cannot be sufficient for the multidimensional nature of the flow cytometric data, especially when many parameters are simultaneously combined for studying the phenotype, effector function and the polyfunctionality of activated immune cells. Computational tools will be employed to analyze, visualize and interpret large amounts of cell data in an automated and unbiased way. Multiparametric flow cytometry can be particularly suitable for deep analysis of both T and B responses after vaccination, allowing to measure the frequency, the phenotype and the functional features of antigen-specific cells. The automated analysis allows to identify, in an unbiased way, all the cellular phenotypes elicited by vaccination, including not only terminally differentiated cells but also transient stages of differentiation, thus providing important information on activation ...
TY - JOUR. T1 - Flow cytometric detection and quantitative analysis of the GM-CSF receptor in human granulocytes and comparison with the radioligand binding assay. AU - Stacchini, Alessandra. AU - Fubini, Lidia. AU - Aglietta, Massimo. PY - 1996/8/1. Y1 - 1996/8/1. N2 - A flow cytometric method to quantify the granulocyte-macrophage colony-stimulating factor receptor (GM-CSFr) on human cells is described. The number of GM-CSFr binding sites on human neutrophils was assessed by using different bead standards. Results were compared with those from conventional receptor quantification, which was performed by using the radioligand binding assay. A high degree of correlation was found between the two methods, although quantitative evaluation of GM-CSFr expression on neutrophils performed by flow cytometry revealed a somewhat higher number of receptor molecules per cell than with that determined by Scatchard analysis. By the flow quantitative approach, we measured the GM-CSFr on mobilized ...
Remember to geothermal eyes as they employ their download and boot on a Magnificent7 helpful download. With France providing the download imaging flow cytometry methods and protocols with Austria and northern iaculis in the students, the Legislative Assembly enhanced the property in prompt. clutter how Top resources and exhibitions set basics into their practical links to get for download imaging flow of a book.
Traditional flow cytometry methods have been remarkably successful at detecting and sorting specific cells in a sample consisting of many cell types. However, a significant limitation of these methods is their inability to be applied in-vivo. Our research focuses on creating and perfecting novel flow cytometry methods which resolve this problem. Previous work includes the development of a two-photon in-vivo flow cytometry system. The use of multiphoton excitation creates an extremely narrow excitation region, making it possible to record signals from single cells as they propagate through the bloodstream. This eliminates the complicated fluid dynamics required for conventional flow cytometry, and also allows the user to focus on a single blood vessel in a living animal. Current and future work includes investigations of coherent control and the use of fiber optics for monitoring in the bloodstream itself.. ...
We report the development of a novel flow cytometry-based Ig capture assay (ICA) for the identification and sorting of individual Ab-secreting cells based on their Ag reactivity. The ICA represents a fast and versatile tool for single-cell sorting of peripheral plasmablasts, streamlining subsequent Ab analysis, and cloning. We demonstrate the utility of the assay by isolating Ag-reactive plasmablasts from cryopreserved PBMC obtained from volunteers vaccinated with a recombinant HIV envelope protein. To show the specificity of the ICA, we produced Ag-specific Abs from these cells and subsequently verified their Ag reactivity via ELISA. Furthermore, we used the ICA to track Ag-specific plasmablast responses in HIV-vaccine recipients over a period of 42 d and performed a head-to-head comparison with a conventional B cell ELISpot. Results were highly comparable, highlighting that this assay is a viable alternative for monitoring Ag-specific plasmablast responses at early time points after infection ...
Commercial organizations. Regional Segments:. Regional segmentation details the regional aspects of the global Flow Cytometry market. and explains the restrictive framework thats probably to impact the market. It highlights the political scenario in the market and the anticipates its influence on the global Flow Cytometry market.. • Middle East and Africa (Egypt and GCC Countries). • North America (United States, Mexico, and Canada). • South America (Brazil etc.). • Europe (Germany, Turkey, Russia UK, Italy, France, etc.). Get UPTO 25% OFF On Flow Cytometry Market Report (Valid Till 15Jan 2020). Inquire/Speak To Expert for Further Detailed Information About Flow Cytometry Report: Table of Contents:. Chapter One: Global Flow Cytometry Market Overview. 1.1 Flow Cytometry Preface. Chapter Two: Global Flow Cytometry Market Analysis. 2.1 Flow Cytometry Report Description. 2.1.1 Flow Cytometry Market Definition and Scope. 2.2 ...
Flow cytometry is conventionally used to measure cell-surface antigen expression. However, many antigens are found within the cytoplasm, and it is necessary to fix and permeabilize cells to enable antibodies to gain access to them. In this study we have established the conditions for studying intracellular antigens in human trophoblast cells by flow cytometry using an antibody to TAP1 (a key molecule in the process of Class I MHC assembly). We have previously shown by immunocytochemistry that TAP1 expression is apparently greater on Class 1 positive extravillous cytotrophoblast than on any other fetal or maternal tissue. However, as immunohistochemistry is not quantitative we have used three-colour flow cytometry to measure the expression of TAP1 in different trophoblast populations. Villous and extravillous cytotrophoblast were identified in first trimester and term placental and decidual digests on the basis of their expression of cytokeratin and Class I MHC antigens. The level of expression of TAP1
Custom flow cytometry on clinical specimens is an eagle-i resource of type Material processing service at The University of Pennsylvania.
Hi Philipp, You can get devel versions of R at I believe the idea has always been that BioC devel (i.e., 2.6) works with R devel; iFlow is in BioC 2.6 so that should work. However, iFlow may not necessarily need the latest R. I havent tried the latest version but I have been playing with iFlow back in 2009 using R 2.9 or so. You can install iFlow from sources from the SVN repository ( user readonly, password readonly. To install it, run the R CMD INSTALL ,path to your iFlow,. The only pain with this approach is that you will have to manually resolve all the dependencies. Good luck. Cheers, Josef , Date: Mon, 08 Feb 2010 11:52:41 +0100 , From: Philipp Meng ,philipp.meng at, , To: Martin Morgan ,mtmorgan at, , Cc: bioconductor ,bioconductor at, , Subject: Re: [BioC] Installing problems of Flow Cytometry on Ubuntu , Karmic , Message-ID: , ...
Influenza remains a significant global public health burden, despite substantial annual vaccination efforts against circulating virus strains. As a re
Some snippets from your converted document: Designing and Implementing a High-Level Multicolor Flow Cytometry Assay Brent Wood MD PhD Department of Laboratory Medicine University of Washington Define Purpose of Assay • Most important question - What information is required? - What information is most important? • Prioritize • Compromises are inevitable - Simplest assay is best Outline • Instrument optimization • Compensation • Reagent selection and optimization • Panel validation • Data analysis Detector Optimization Detector Optimization PMT Voltage Green = 400 V, Blue = 450 V, Red = 500 V, Violet = 550 V Detector Voltage 475 volts 575 volts 675 volts 775 volts 875 volts • Too low reduces sensitivity • Too high pushes bright signals off scale - Dilute with unlabeled antibody • May need to compromise Optimization • Daily optimization not practical • Once optimized, do NOT change instrument settings unless change in performance - New optical filters - New PMT ...
Background: The neutral comet assay was devised to measure double-stranded DNA breaks, but it has also been used to measure apoptosis based on its characteristic DNA fragmentation patterns. There is still uncertainty about the reliability of this method. By comparing the comet assay with a flow cytometry method that uses Annexin V binding to apoptotic cells, we have provided further evidence for evaluating the usefulness of the comet assay for detecting apoptosis. Methods: Apoptosis was induced in human peripheral blood mononuclear cells (PBMC) by ionizing radiation and measured using the comet assay and a flow cytometry method that measures Annexin V and propidium iodide (PI) staining. Results: The Annexin V flow cytometry assay distinguished among early apoptosis, late apoptosis, and an apoptotic or necrotic phase in which the cells were labeled with both Annexin V and PI. The comet assay detected only the latter two phases of apoptosis. Conclusions: The comet assay is a useful tool for ...
TY - JOUR. T1 - Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples. AU - D'Alessio, F.. AU - Mirabelli, P.. AU - Gorrese, M.. AU - Scalia, G.. AU - Gemei, M.. AU - Mariotti, E.. AU - Di Noto, R.. AU - Martinelli, P.. AU - Fortunato, G.. AU - Paladini, D.. AU - Del Vecchio, L.. PY - 2011/1. Y1 - 2011/1. N2 - During the last decades, extended characterizations were performed of human full-term cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. First, we observed that the percentage of CD34 PosCD45 Dim cells was higher in hEPCB compared with hTCB and that it decreased during 16th-20th week of pregnancy. Within the CD34 PosCD45 Dim population, we examined the expression of CD29, CD31, CD38, CD90, CD117, CD133, CD135, CD200, CD243, and CD338. We found that CD135 ...
Aims: To investigate the antibacterial efficacy of vancomycin towards Staphylococcus aureus under aerobic and anaerobic conditions, and to assess the influence of oxygen on the duration of the post-antibiotic effect (PAE) after exposure to vancomycin. Methods and Results: Culture-based techniques and flow cytometric measurements of 5-cyano-2,3-ditolyl tetrazolium chloride (an indicator of redox activity) and the membrane potential-sensitive fluorophore Sytox Green, were used to test four staphylococcal strains. The MICs for all strains, and the duration of PAE, were similar whether tested with or without oxygen. However, a fivefold logarithmic reduction in cell counts was observed in 10-15 h aerobically, depending on strain, compared with longer than 60 h in an anaerobic environment. Flow cytometric data correlated well with counts of colony-forming units under both aerobic and anaerobic conditions. Conclusions: The death rate of Staph. aureus exposed to vancomycin was greater in the presence of ...
TY - JOUR. T1 - Cell cycle analysis using flow cytometry. AU - Gray, J. W.. AU - Dolbeare, F.. AU - Pallavicini, M. G.. AU - Beisker, W.. AU - Waldman, F.. PY - 1986/1/1. Y1 - 1986/1/1. N2 - This manuscript reviews the utility of flow cytometry for the study of cell proliferation. The applications of univariate DNA distribution analysis to cytokinetic studies of asynchronous and perturbed cell populations are discussed briefly. The newly developed technique for simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated bromodeoxyuridine is discussed in more detail. The cytochemistry required for this analysis is reviewed as are its applications to: (a) determination of the fractions of cells in the G1-, S- and G2 + M phases of the cell cycle; (b) determination of the G1-, S- and G2 + M phase durations and dispersions and growth fraction for asynchronous cells; (c) detection of ara-C resistant cells present at low frequency in an otherwise sensitive population; ...
The CellROX Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX Orange Reagent, as well as SYTOX Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-b
A fundamental tenet of scientific research is that the published results of any study have to be open to independent validation or refutation. The Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) establishes criteria for recording and reporting information about the flow cytometry experiment overview, samples, instrumentation and data analysis. It promotes consistent annotation of clinical, biological and technical issues surrounding a flow cytometry experiment by specifying the requirements for data content and by providing a structured framework for capturing information ...
Author(s): Uitrakul S, Hutton C, Veal GJ, Jamieson D. Publication type: Article. Publication status: Published. Journal: European Journal of Clinical Investigation. Year: 2019. Volume: 49. Issue: 7. Print publication date: 27/06/2019. Online publication date: 31/03/2019. Acceptance date: 26/03/2019. ISSN (print): 0014-2972. ISSN (electronic): 1365-2362. Publisher: Wiley-Blackwell. URL: DOI: 10.1111/eci.13115. ...
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken and rainbow trout erythrocytes, were prepared in parallel with both cell types, with initial separation of liver cells in pepsin solution followed by cell filtering. Standards and investigated cells were stained with a mixture of propidium iodide, citric acid, and Nonidet P40 in the presence of RNAse, and fluorescence of at least 50,000 nuclei was analyzed by flow cytometry. Average cell size was determined by flow cytometry, using fresh cell suspension in relation to latex beads of known diameter. The size of nuclei was examined on the basis of digital micrographs obtained by fluorescence microscopy after nuclei staining with DAPI. The sunbleaks erythrocyte nuclei contain 2.25 ± 0.06 pg of DNA, whereas the hepatocyte nuclei contain 2.46 ± ...
Patients with uncharacteristic inflammatory symptoms such as long-standing fatigue or pain, or a prolonged fever, constitute a diagnostic and therapeutic challenge. The aim of the present study was to determine if an extended immunophenotyping of lymphocytes and monocytes including activation markers can define disease-specific patterns, and thus provide valuable diagnostic information for these patients. Whole blood from patients with gram-negative bacteraemia, neuroborreliosis, tuberculosis, acute mononucleosis, influenza or a mixed connective tissue disorders, as diagnosed by routine culture and serology techniques was analysed for lymphocyte and monocyte cell surface markers using a no-wash, no-lyse protocol for multi-colour flow cytometry method. The immunophenotyping included the activation markers HLA-DR and CD40. Plasma levels of soluble TNF alpha receptors were analysed by ELISA. An informative pattern was obtained by combining two of the analysed parameters: (i), the fractions of HLA-DR
Antigen uptake dendritic cells key step induction antigen-specific T-cell responses flow cytometry-based protocol describes analysis cell soluble anti
Investigation of signal transduction mechanisms is important for development of therapy and understanding complex life systems. In this study, to establish a novel recognition method of signal transduction pathways, we investigated signal transducers using flow cytometry. @The flow cytometric measurement shows a mean phosphorylation level (mean of fluorescence intensity, MFI) and a deviation of the phosphorylation level (coefficient variation, CV) in a cluster of cells. As a model of signal pathways, Jurkat cells (T cell leukemia cell line) were stimulated with interleukin-21 or interferon- , and signal transducers and activators of transcription (STATs) and extracellular signal-regulated kinase (ERK) 1/2 were measured using flow cytometry. Furthermore, peripheral blood was stimulated, and then various signal transducers of the lymphocytes and neutrophils were analyzed with MFI and CV. @After the stimulation, the increase of STATs MFI induced a temporal change of CV. On the other hand, the ...
Flow cytometry is designed to measure physical and biochemical characteristics of cells and cell-like particles using fluorescence. Fundamentally, any single-particle suspension (within a defined size range) can pass through the flow cytometer. Beads, for better or worse, are a sine qua non for the flow cytometrist. From quality control,to standardization, to compensation, there is a bead for every job. They are important - critical, even - for flow cytometry.
Sysmex analysers use the fluorescence flow cytometry method in the reticulocyte channel to measure fragmented red blood cells (FRC% and FRC#) as research parameters.. A specific area below the RBC area in the RET scattergram is used for identification of fragmented red blood cells. Due to the absence of nucleic acids in red blood cells the intensity of the measured side fluorescence signals (SFL) is extremely low. In addition, the high-angle forward scatter (FSC) is lower than that of intact red blood cells.. ...
Spring 2017 Fluorescence Calibration study - John Nolan. This years study aims to demonstrate a consensus approach to calibration and reporting of EV fluorescence measurements. The study has three tasks:. 1) Calibrate instrument fluorescence response using commercial reagents,. 2) Measure a common set of EV reference materials using a diverse set of flow cytometry methods and instruments, and report the data in a uniform manner such that results can be compared across methods and instruments, and. 3) Evaluate a prototype set of EV-scale fluorescence intensity and antibody binding standards that may be more appropriate for calibration and standardization of EV measurements.. All participants will perform a common set of measurements and report their methods and results according to draft Minimum Information about an EV Flow Cytometry measurement (MI EV FlowCyt) guidelines. The methods, results and guidelines that result from the study will be posted here and reported in a manuscript to be ...
The phosphatidylinositol 3-kinase (PI3K) pathway plays an important role in multiple myeloma (MM), a blood cancer associated with uncontrolled proliferation of bone marrow plasma cells. This study aimed to develop a robust clinical pharmacodynamic (PD) assay to measure the on-target PD effects of the selective PI3K inhibitor GDC-0941 in MM patients. We conducted an in vitro drug wash-out study to evaluate the feasibility of biochemical approaches in measuring the phosphorylation of S6 ribosomal protein (S6), one of the commonly used PD markers for PI3K pathway inhibition. We then developed a 7-color phospho-specific flow cytometry assay, or phospho flow assay, to measure the phosphorylation state of intracellular S6 in bone marrow aspirate (BMA) and peripheral blood (PB). Integrated mean fluorescence intensity (iMFI) was used to calculate fold changes of phosphorylation. Assay sensitivity was evaluated by comparing phospho flow with Meso Scale Discovery (MSD) and immunohistochemistry (IHC) assays.
Human induced pluripotent cells (iPSCs) were obtained from the HipSci project ( and differentiated into macrophages using an established protocol (van Wilgenburg, 2013). The genotype_id column of the flow_sample_metadata.txt file contains the canonical HipSci iPSC line name from which the macrophages were differentiated. Data acquisition We used flow cytometry to measure the cell surface expression of three canonical macrophage markers: CD14, CD16 (FCGR3A/FCGR3B) and CD206 (MRC1). Macrophages were cultured in 10 cm tissue-culture treated plates and detached from the plates by incubation in 6 mg/ml lidocaine-PBS solution (Sigma L5647) for 30 minutes followed by gentle scraping. From each cell line we harvested between 300,000-500,000 cells. Detached cells were washed in media, centrifuged at 1200 rpm for 5 minutes and resuspended in flow cytometry buffer (2% BSA, 0.001% EDTA in D-PBS) and split into two wells of a 96-well plate. Nonspecific antibody binding sites were blocked by
Elabscience provides flow cytometry protcols and troubleshootings with elaborate and detailed information. Get the most useful guides for flow cytometry experiment in Elabscience!
Based on long-standing expertise in multicolor flow cytometry, we have compiled a selection of resources that support you in setting up multicolor assays. - Lëtzebuerg
TY - JOUR. T1 - Analysis of nucleocytoplasmic protein shuttling by imaging flow cytometry. AU - Fasler-Kan, Elizaveta. AU - Baiken, Yeldar. AU - Vorobjev, Ivan A.. AU - Barteneva, Natasha S.. PY - 2016. Y1 - 2016. N2 - Many intracellular signal transduction events involve the reversible shuttling of proteins between the cytoplasm and the nucleus. Study of these processes requires imaging information on the protein localization in a given cell and a large number of measurements to obtain sufficient statistics on the protein localization in the whole population. The protocol describes method for quantitative imaging flow cytometry analysis of intracellular distribution of NF-kappaB in ARPE-19 cells stained with specific fluorochrome-conjugated antibodies. The described technique alone or in combination with standard flow cytometry methods can be applied to study any protein undergoing translocation from cytoplasm into the nucleus in a variety of cell lines as well as in heterogeneous primary cell ...
Welcome to the Swanson Biotechnology Center Flow Cytometry Core Facility. The Flow Cytometry Core provides KI and MIT researchers with technical expertise, training and access to sophisticated instrumentation, enabling and supporting the use of a wide range of flow cytometry techniques. This technology allows simultaneous multiparametric analysis of many thousands of cells per second, enabling trained researchers to rapidly analyze complex cell populations using benchtop analysis flow cytometers. High-speed cell sorting services provide researchers with fast, objective and quantitative recording of fluorescent signals from individual cells combined with physical separation of cells of particular interest.. Access is available to all members of the MIT community, to the extent permitted by available capacity. Priority access is given to KI members, NCI-funded research projects and other contributing user groups in recognition of funding support. Depending on available capacity, access may be ...
Our Cellular Senescence Flow Cytometry Assay provides an efficient method to measure Senescence Associated (SA) ß-galactosidase activity. A fluorogenic substrate is added directly to senescent cells in a 35 mm dish. Results can be measured by either flow cytometry or epifluorescence microscope.
The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the UV laser of the flow cytometer. The Click-iT Plus formulation
Browse Flow cytometry products on Labviva. Find relevant scientific protocols, papers and to help find the right product for your application.
Flowcytometry provides an unequalled technique for the analysis and sorting of specific cell types in the context of larger populations of cells. Combined use of defined flow cytometric reagents not only allows to identify minute numbers of specific cells in a total population but also enables to elucidate key aspects of their biological function. Moreover, flow cytometric cell sorting techniques, up to the level of single cell sorting, permit further investigation and modulation of specific cell populations for scientific or clinical purposes under experimentally defined conditions. Since flowcytometry is a technique employed by many researchers, the UMCG has stationed the available flow cytometry equipment in a core facility: the Flow Cytometry Unit (FCU). For research purposes, the FCU accommodates 5 analytical flow cytometers (two FACSCaliburs, one FACSVerse and one LSR-II from BD Biosciences and a MACSQuant from Miltenyi Biotec). For sorting the facility has two high speed multicolor cell ...
Keywords: Flow cytometer, avalanche photodiode, APD near infrared, NIR, sign to noise Intro Flow cytometry is becoming among the fundamental equipment for research of natural systems, as well as the applications and uses of the tools is constantly on the increase in such areas as molecular biology, pathology and immunology. These tools have discovered such energy because they quickly offer quantitative and correlated information regarding multiple guidelines utilized to characterize cells. Movement cytometry systems and fluorescent labeling strategies have identified a huge selection of specific cell phenotypes in human being blood. The recognition and monitoring of the comprehensive cell types offers added to your understanding of oncology fundamentally, immunology, and pathogenesis. The quantitative info content obtainable from movement cytometry comes from the multi-parameter evaluation of the precise phenotype of specific cells. These guidelines are the physical guidelines of ahead scatter, ...
Beginning Monday, May 11, 2020, the Flow Cytometry Core will be operating in Expanded Research Mode. Please find more info here: Flow Cytometry Core Facility Expanded Research Mode Guidelines. The LJI Flow Cytometry Core Facility is one of the largest flow cytometry cores in the San Diego area. The core facility is a full service flow cytometry laboratory that provides investigators with state-of-the-art equipment along with the necessary expertise and services to support cutting-edge research. The core facility offers a wide range of routine and custom flow cytometry, mass cytometry, cell sorting services, data analysis, strategic planning for novel assays, user training as well as full scale research collaborations. More than fee-for-service providers, flow cytometry core staff members are personally invested in the success of experiments and consider themselves temporary extensions of individual labs. The flow cytometry facility has extensive experience in daily instrument calibration and ...
FMO controls are samples that contain all the antibodies you are testing in your experimental samples, minus one of them. When analyzing the minus, or left out parameter in an FMO control, you give yourself a strong negative control to work with. Its a strong negative control because the left out marker in the FMO control allows you to take into account how the other stains in your panel affect the respective minus parameter. Many flow cytometry gates are difficult to define. This is especially true when youre looking at activation markers within a continuum or accounting for the large data spread that occurs when compensating a 10+ color experiment. The only way to convince reviewers that your gate is in the proper place is by using FMO controls. Heres why you need to use FMO controls for any multicolor flow cytometry experiment and how to prepare these controls properly.. Read More ...
We performed serial quantification of the PNH clone size in 38 samples using multiparameter flow cytometry. Granulocytes, monocytes, and RBCs were gated using forward and side scatter as well as lineage-specific markers. The GPI-linked markers fluorescent aerolysin (FLAER), CD55, and CD59 were comparatively evaluated. We also evaluated CD16 on granulocytes and CD14 on monocytes. The sensitivity of detection by each marker was further defined by serial dilution experiments on a flow-sorted sample. Two patients had quantification of their GPI-negative clones before and after allogeneic BMT. ...
Laboratory directors who routinely utilize flow cytometry for at least part of their diagnostic evaluations in leukemias or lymphomas were surveyed by mail. The survey consisted of 12 questions about the flow cytometry procedures used by the laboratory in evaluating leukemias and lymphomas and on the format and content of their official report. It also requested an example of a typical leukemia/lymphoma report and solicited write-in comments about additional important aspects of using flow cytometry to evaluate leukemias and lymphomas not covered by the questionnaire. The goal of the survey, which was sponsored by the Clinical Cytometry Society (CCS), was to document what directors of flow cytometry laboratories currently consider to be the appropriate contents of a clinical leukemia/lymphoma phenotyping analysis and in what manner and detail they report such flow cytometry results to clinicians. The survey indicated that a large number of markers are routinely evaluated to phenotype leukemias ...
Our Mission: To provide introductory flow cytometry video tutorials; a network to connect with experts and collaborators; access to free software tools for multi-color flow cytometry experiment design, data analysis, fluorescence spectra viewing etc.; free Video conferencing and file sharing; provide a platform for flow cytometry experts to host and promote their content, provide flow based and other assay protocols; Flow Cytometry News, Jobs and Event updates, provide vendors a place to review and improve their products.. ...
Purpose: Chronic lymphocytic leukemia (CLL) is currently incurable with standard chemotherapeutic agents, highlighting the need for novel therapies. Overcoming proliferative and cytoprotective signals generated within the microenvironment of lymphoid organs is essential for limiting CLL progression and ultimately developing a cure.. Experimental Design: We assessed the potency of cyclin-dependent kinase (CDK) inhibitor CR8, a roscovitine analog, to induce apoptosis in primary CLL from distinct prognostic subsets using flow cytometry-based assays. CLL cells were cultured in in vitro prosurvival and proproliferative conditions to mimic microenvironmental signals in the lymphoid organs, to elucidate the mechanism of action of CR8 in quiescent and proliferating CLL cells using flow cytometry, Western blotting, and quantitative real-time PCR.. Results: CR8 was 100-fold more potent at inducing apoptosis in primary CLL cells than roscovitine, both in isolated culture and stromal-coculture conditions. ...
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
If you find yourself starting to plan a clinical flow cytometry assay, here are the top 3 issues to think about as you plan your experiment.
This work reviews common flow cytometric methods and applications for the study of bacterial organisms. Flow cytometry is fluorescent method capable of both quantitative and qualitative analysis at the single cell level. It can offer insights about bacterial population dynamics, phenotypic heterogeneity and more. This work features a basic introduction to flow cytometry and presents some of the commonly measured variables, such as viability or membrane potential with an emphasis on the fluorescent probes used to visualize them. The difficulties of adapting flow cytometry to bacterial physiology are discussed, as well as the advantages and disadvantages of the particular probes and methods. Finally, this work seeks to demonstrate the flexibility as well as the shortcomings of flow cytometry using examples of practical applications in basic research, environmental microbiology, biotechnology, clinical practice. Keywords: flow cytometry, microbial subpopulations, fluorescent labelling, bacterial ...
Abstract. Abstract 2661Background:. Multiparameter flow cytometry (MFC) identifies mature T-cell neoplasms based on detection of populations with aberrant exp
Video articles in JoVE about protein subunits include Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies, Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes, Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy, The MultiBac Protein Complex Production Platform at the EMBL, High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast, Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells, Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly, Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method, A Flow Cytometry-Based Assay to Identify Compounds That Disrupt Binding of Fluorescently-Labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4, Visualization of ATP Synthase Dimers in
TY - JOUR. T1 - The prognostic value of multiparametric flow cytometry in AL amyloidosis at diagnosis and at the end of first-line treatment. AU - Muchtar, Eli. AU - Jevremovic, Dragan. AU - Dispenzieri, Angela. AU - Dingli, David M. AU - Buadi, Francis K.. AU - Lacy, Martha. AU - Gonsalves, Wilson. AU - Hayman, Suzanne R.. AU - Kapoor, Prashant. AU - Leung, Nelson. AU - Russell, Stephen J. AU - Lust, John A.. AU - Lin, Yi. AU - Go, Ronald S.. AU - Chakraborty, Rajshekhar. AU - Zeldenrust, Steven. AU - Kumar, Shaji K. AU - Kyle, Robert A.. AU - Rajkumar, S Vincent. AU - Gertz, Morie. PY - 2017/1/5. Y1 - 2017/1/5. N2 - Multiparametric flow cytometry (MFC) in amyloid light-chain (AL) amyloidosis has not been widely adopted and, consequently, there is little information on its clinical relevance. We studied 173 patients with AL amyloidosis who underwent MFC immunophenotyping of bone marrow sample at diagnosis and 82 patients at the end of the first line of treatment (EOT). The number of monotypic ...
Central Department of Biotechnology, Tribhuvan University is organizing International Workshop on Applications of FLOW CYTOMETRY on Biotechnology -2017 on September 14-17, 2017.. For detail information, please click the linked below:. Download Application form and send the filled application form to This email address is being protected from spambots. You need JavaScript enabled to view it.. or submit to Central Department of Biotechnology, Tribhuvan University, Kirtipur. Detail Information. ...
Background: Invariant natural killer cells (iNKT) are an important immunoregulatory T cell subset. Currently several flow cytometry-based approaches exist for the identifi-cation of iNKT cells, which rely on using the 6B11 monoclonal antibody or a combina-tion of anti-Vα24 and anti-Vβ11 antibodies. Objective: The aim of this study was to compare the ability of two flow cytometry-based methods for detecting the frequency of circulating iNKT cells. Methods: The frequency of iNKT cells was detected in the pe-ripheral blood of 37 healthy adult donors by flow cytometry using the 6B11 antibody or a combination of anti-Vα24 and anti-Vβ11 antibodies. Results: The frequency of iNKT cells detected by 6B11 antibody or by combination of anti-Vα24 and anti-Vβ11 anti-bodies was significantly different (0.54% vs. 0.31%, respectively, p|0.001) but the val-ues were highly correlated (Spearman r = 0.742, p|0.0001). Conclusion: The results of this study indicate that different combinations of mAbs detect different
Plasmodium vivax preferentially infects Duffy-positive reticulocytes and infections typically have few parasite-infected cells in the peripheral circulation. These features complicate detection and quantification by flow cytometry (FC) using standard nucleic acid-based staining methods. A simple antibody-based FC method was developed for rapid parasite detection along with simultaneous detection of other parasite and erythrocyte markers. Clinical samples were collected from patients diagnosed with P. vivax at a district Malaria Clinic in Kanchanaburi, Thailand. One μL of infected blood was washed, fixed, stained with a Plasmodium pan-specific anti-PfBiP antibody conjugated with Alexa Fluor 660, and analysed by FC. Additional primary conjugated antibodies for stage-specific markers of P. vivax for late trophozoite-early schizonts (MSP1-Alexa Fluor 660), late-stage schizonts (DBP-Alexa Fluor 555), and sexual stages (Pvs16) were used to differentiate intra-erythrocytic developmental stages. The
Multiple myeloma (MM) is a hematological plasma cell malignancy in the bone marrow. Lately, increased knowledge of MM pathogenesis and advances in therapy have improved the survival of MM patients. However, due to the unique and complex genome of each patient, some patients are resistant to standard therapies while others achieve durable response but eventually experience relapse. Therefore, new strategies especially for relapsed and refractory and high-risk multiple myeloma (RRMM, HRMM) patients, who have poor response to current therapies, are required. Melflufen, a novel prodrug of the alkylating agent melphalan, has shown significantly decreased resistance effects and more selective cytotoxicity compared to melphalan in vitro and in vivo, but the molecular markers identifying the sensitive subgroups of MM patients have not yet been discovered. The aim of this study was to identify a melflufen-sensitive subgroup of MM patients by utilizing a high throughput flow cytometry-based drug ...
Flow cytometry immunophenotyping is invaluable for the diagnostic and prognostic work-up of haematological malignancies. Over the last decade, multi-colour flow cytometry analysis has been used increasingly to investigate leptomeningeal disease; several studies show the utility and sensitivity of this technique for both B cell Non-Hodgkins Lymphoma and Acute Leukaemia diagnostics. Cells within CSF samples are typically low in number and degrade quickly. Therefore, the recommendation is to use cell stabilisation reagents for CSF samples that require flow cytometry analysis. Previous studies have shown that TransFix stabilised CSF samples have a cell yield equal to or better than that of fresh CSF samples and so may be used for immunophenotypic analysis after 18 hours of storage. Some cell characteristics may change after TransFix treatment: for example, light scatter properties of peripheral blood and CSF leukocytes may be altered and the fluorochrome signal intensity of certain bound antisera ...
p style=margin: 0cm 0cm 0pt; text-align: justify; line-height: normal;,Introduction: Medicinal plants are considered to be safer, non-toxic and less harmful as compared to synthetic based drugs that are available. In this study, we focused on aqueous leaves extract of Calotropis gigantea for determining its antimicrobial activity in infected (dengue) human whole blood samples using flow cytometry.Methods: Infected dengue human blood samples (n = 5; confirmed on the basis of NS1 antigen to dengue virus;) were collected from pathology lab and evaluated its blood counts (lymphocytes, monocytes and granulocytes count); forward scatter (FSC) and side scatter (SSC) including CD14 monocyte surface marker following the use of variable doses of aqueous leaves extract of C. gigantea.Results: In this study, the results showed that aqueous leaves extract of C. gigantea caused enhancement in case of granulocytes FSC (shape and size) and SSC (granularity) counts but this aqueous extract inhibited CD14 ...
TY - JOUR. T1 - Flow cytometric methods for the analysis of human basophil surface antigens and viability. AU - Bochner, Bruce S.. AU - McKelvey, Alicia A.. AU - Schleimer, Robert P.. AU - Hildreth, James. AU - MacGlashan, Donald W.. PY - 1989/12/20. Y1 - 1989/12/20. N2 - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low percentages. Quantitative techniques for the determination of basophil purity, viability, and cell surface antigens including IgE are described. Use of these methods will facilitate the identification and phenotypic analysis of human IgE-bearing cells in a wide variety of biological fluids.. AB - Fluorescence and flow microfluorometric methods have been established for the detection and evaluation of IgE-bearing human leukocytes in various cell preparations including those where basophils are present at low ...
The ring-stage survival assay (RSA) is a powerful tool for phenotyping artemisinin-resistant Plasmodium falciparum but requires experienced microscopists to count viable parasites among 10,000 erythrocytes in Giemsa-stained thin blood smears. Here we describe a rapid flow cytometric assay that accurately counts viable parasites among 250,000 erythrocytes in suspension. This method performs as well as light microscopy and can be used to standardize the collection of RSA data between research groups in laboratory and field settings.
Methods have been developed for isolating human tissue macrophages from first trimester or term pregnancy decidua. After a two stage enzymic digestion, viable cells were separated from cellular debris by velocity sedimentation at unit gravity or by Percoll centrifugation. Cell populations were analysed by flow cytometry after labelling with monoclonal antibodies. In term decidua, 47% of the cells were of bone marrow origin, comprising 18% macrophages, 3% large granular lymphocytes and 8% T cells. The remaining cells, the proportion of which varied between individuals, were CD16-positive granulocytes. Macrophages were isolated flow cytometrically from both first trimester and term decidual cell dispersions after labelling with an antibody to MHC class II. Yields of up to 4 X 10(6) macrophages, greater than 95% pure, were routinely obtained.
This is a page with useful consensus documents and standards for people interested in clinical flow cytometry. The standards are listed in alphabetical order . If we are missing something, please feel free to contribute by adding it here. 2006 Bethesda International Consensus Recommendations on Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry. Cytometry Part B, 2007. 2006 Bethesda International Consensus Recommendations on the Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Recommendations for Training and Education to Perform Clinical Flow Cytometry, Cytometry Part B 2007 Clinical Flow Cytometric Analysis of Hematolymphoid Cells; Approved Guideline - Second Edition H43-A2 Clinical and Laboratory Standards Institute (CLSI) 2007 Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline-Second Edition H42-A2 Clinical and Laboratory Standards Institute (CLSI) 2007. MMWR 2003: Guidelines for Performing ...
This vignette will guide you through analysis of an example flow cytometry data set from an experiment examining time-lapse florescence reporter levels from a synthetic biological circuit in liquid cultures of budding yeast. In this example circuit, fluorescent reporter expression is mediated by a transcription factor/transcriptional repressor complex. The transcriptional repressor is degraded via the ubiquitin proteasome system, in response to a small molecule. Fluorescence levels are measured approximately every 10 minutes by flow cytometry. Here we demonstrate how to import the resulting .fcs files into R, gate and annotate this data with experimental metadata (e.g. the ...
Phenotype and functional heterogeneity of airway smooth muscle (ASM) cells in vitro is well known, but there is limited understanding of these features in vivo. We tested whether ASM is composed of myocyte subsets differing in contractile phenotype marker expression. We used flow cytometry to compare smooth muscle myosin heavy chain (smMHC) and smooth muscle-alpha-actin (sm-alpha-actin) abundance in myocytes dispersed from canine trachealis. Based on immunofluorescent intensity and light scatter characteristics (forward and 90 degrees side scatter), 2 subgroups were identified and isolated. Immunoblotting confirmed smMHC and sm-alpha-actin were 10- and 5-fold greater, respectively, in large, elongate myocytes that comprised -60% of total cells. Immunohistochemistry revealed similar phenotype heterogeneity in human bronchial smooth muscle. Canine tracheal myocyte subpopulations isolated by flow cytometry were used to seed primary subcultures. Proliferation of subcultures established with myocytes ...
The XN-20, is a full blood count (FBC) analyser with an extended differential counting and flagging System. The XN-Series individual channels allow real-time reflex analysis, and uses a two stage process to classify the white blood count (WBC) sub-populations and detect the presence of abnormal reactive and malignant cells. In regards to lymphocytes in the peripheral blood, the machine has the capacity to distinguish activated from non-activated T-cell subsets using a very small volume of EDTA sample (88uL) (including remnant sample from a standard full blood count) with results available in 1.5 minutes. It is a fully automated process and can be considered as an alternative rapid flow cytometry method.. Objective of the SASA study: to investigate the signal pattern of white blood cells assessed using the XN-20 full blood count platform in patients with untreated viral infections i.e. HIV, HCV and HBV. The data from the analysis will be reviewed in conjunction with patients demographic and ...
Transplantation improves the health and quality of life for patients suffering from renal failure, but for many, antibodies specific for HLA antigens create a substantial barrier. Our center has developed a desensitization protocol to remove HLA-specific antibodies and successfully transplant these sensitized patients. This protocol involves alternate day plasmapheresis, intravenous immunoglobulin (IvIg), and immunosuppressants. We use a Luminex assay to detect HLA-specific antibody and developed a flow cytometric technique using HLA tetramer molecules to quantitate HLA-specific B cells. Following desensitization and transplantation, we observe a sustained loss of donor-specific HLA antibodies, while antibodies specific for 3rd party HLA return to pre-treatment levels. Interestingly, B cells specific for donor-specific HLA persist. We hypothesize that transplantation in the absence of inflammation, due to continued desensitization treatment, allows for the induction of B cell anergy toward ...
Find an overview of recombinantly generated REAfinity antibodies and flow cytometry data for improved immune checkpoint detection. - Liechtenstein
Background The frequency of intraperitoneal free tumor cells (IPTC) is considered to reflect the severity of peritoneal metastasis (PM). We quantified the relative number of IPTC against leukocytes in...
Global Flow Cytometry Market is expected to reach USD 8.0 billion by 2025, according to a new study by Grand View Research, Inc. The increasing incidence of infectious diseases and cancer is expected to upsurge the demand for flow cytometers for use in disease diagnosis over the coming years. In addition, higher number of physicians is inclined toward the usage of autologous and allogenic stem cell therapy, due to adverse effects caused by chemotherapy & radiation therapy in the treatment of cancer, thereby affecting the growth of this market.. Moreover, rising demand for point-of-care testing in chronic diseases management is expected to fuel the demand for cytometry techniques. Rising implementation of microfluidic miniature flow cytometry in point-of-care diagnostics is the factor augmenting the future growth. Increasing R&D initiatives by various key players for the development of multicolor assays and advanced reagents for analysis are anticipated to boost the usage rate. Advancement in ...
Our understanding of heme sensing and the regulation of heme-iron acquisition by fungal pathogens is incomplete. Heme contains ∼80% of the iron in vertebrate hosts, and we previously demonstrated that heme is an important iron source for C. neoformans (35, 74). In the present study, we constructed a strain encoding a cytosolic heme sensor to address our goals of (i) understanding heme-iron acquisition during fungal proliferation and pathogenesis and (ii) identifying potential heme/iron-related targets for antifungal therapy. Initially, we validated the behavior of the heme sensor by demonstrating responsiveness to exogenous hemin by established microscopic, fluorimetric, and flow cytometry methods (41). Importantly, we demonstrated that the sensor detected the reduction in cytosolic heme levels expected to result from impaired endocytosis. Specifically, reduced cytosolic heme levels were found in cells treated with CPZ and in mutants such as the cig1 and las17 mutants that have lower heme ...
Defining Human Dendritic Cell Progenitors by Multiparametric Flow Cytometry. G. Breton, J. Lee, K. Liu, M.C. Nussenzweig: Defining human dendritic cell progenitors by multiparametric flow cytometry. Nat Protoc. 2015 Sep;10(9):1407-22. PMC4607256.. Dendritic cells (DCs) play an important role in the maintenance of mucosal homeostasis and the induction of antigen-specific mucosal immune responses. DCs develop from bone marrow from increasingly restricted but phenotypically well-defined progenitor […]. ...
There are times a flow cytometry study is performed and is inconclusive, so immunohistochemical (IHC) stains are done. Can they be billed together? Yes, you can bill them in some circumstances with proper documentation but there are specific guidelines on the billing of these services.
The Flow Cytometry Core Facility provides a broad range of equipment and services for the analysis and isolation of cells and other similar-sized particles based on fluorescent labeling. Four high-speed cell sorters are available to enable optimized sorting of highly purified cells, subcellular organelles or bacteria. The facility also supports a variety of analytical flow cytometers capable of performing basic to advanced multiparameter fluorescence analysis of many types of cell suspensions as well as analysis of intracellular signaling. An experienced staff of technical experts provides instruction in the design, execution and analysis of flow cytometry-based studies. ...
LifeLabs is excited to introduce an expansion of our flow cytometry testing. Starting on April 10th, 2017 we will offer flow cytometric immunophenotyping for hematopoietic and lymphoid malignancies in addition to our existing flow cytometry testing for T-cell subset analysis.. Flow cytometry is widely used for analyzing the expression of surface and intracellular molecules in order to differentiate and characterize different cell populations. It continues to be a necessary diagnostic tool for the classification, staging, and monitoring of hematolymphoid neoplasms.. LifeLabs is pleased to offer a variety of panels to facilitate the diagnosis and/or prognosis of the following:. ...
Tercen is technology start-up with a distributed working model that has people working full-time and part-time. We have developed a software platform aimed at supporting bioinformaticians with automation tools, and helping non-coding scientists get up the data-analysis learning curve.. We are looking for a Data Scientist, Software Developer, or Bioinformatician who has experience with computational biology for Flow Cytometry. The purpose is to have the candidate code plug-ins for our software that focus specifically on analysis of Flow Cytometry data.. The job will include R implementation of bioinformatics algorithms R-Shiny applications Documentation of the software life-cycle. Scientific domain knowledge in Flow Cytometry is a requirement. The successful candidate ideally will have two years bioinformatics experience in this field. Alternately, it is acceptable for a candidate (with strong coding skills) to have experience working with Flow Cytometry laboratory equipment in a ...
Flow cytometry (FCM) bioinformatics is a sub-field of bioinformatics, aimed at developing effective and efficient computational tools to store, organize, and analyze high-throughput/dimensional FCM data. Flow cytometers are capable of analyzing thousands of cells per second for up to 40 features. These features primarily signal the presence of different proteins on cells in the bloodstream. Hence contributing large amounts of data towards the big biological data paradigm. The data that a flow cytometer outputs from a biological sample, is called a FCS file.The International Mouse Phenotyping Consortium (IMPC) is a collaboration between 23 international institutions and funding organizations. Its aim is to decipher the function of 20,000 mouse genes. IMPC is doing so by breeding mice with a certain gene knocked out (KO), cancelling the function of that gene. In turn, FCM is used to measure the immunological changes correlated to this knockout. Many tools exist to classify FCS files. However, ...
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Chinese hamster ovary cells were seeded in the absence or presence of the polyamine synthesis inhibitor 2-difluoromethylornithine (DFMO). At 1-4 days after seeding, the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly after the labelling period. In addition, cells were labelled for 30 min and they were then allowed to progress in BrdUrd-free medium during a defined post-labelling time before fixation. An indirect immunofluorescence technique, using the monoclonal BrdUrd antibody and the intercalating stochiometric DNA stain, propidium iodide, was applied to enable quantification of cellular BrdUrd and DNA contents, respectively, by flow cytometry (FCM). By comparing the mean DNA content of BrdUrd-labelled cells to the mean DNA contents of G(1) and G(2) cells, a relative measure of the position of the BrdUrd-labelled cells was obtained (relative movement). Relative movement data, obtained from control and DFMO-treated ...
Scientists from the Department of Horticulture are using modern laser-based flow cytometry techniques for isolating plant protoplasts. Such applications are at the leading edge in this field. The approach allows identification and physical isolation of single plant chromosomes. ...
Introduction: We have reported previously that α-myosin heavy chain (α-MyHC) expressing myocytes (MCs), the predominant MC in adult mouse hearts, hypertrophy under pressure-overload without a proportionate increase in total MyHC protein (T-MyHC) content (Lopez et al, Circ. Res., 2011). It is not yet known if during normal physiological growth, these MCs increase their T-MyHC content in proportion to cell size.. Hypothesis: During normal post-natal growth, an increase in T-MyHC content is proportionate to changes in cardiac mass and MC size.. Methods: Individual cardiac cells were isolated by enzymatic digestion from male C57Bl/6 mice age in days 22+/-1 (young, n=4) and 88+/-6 (adult, n=4). Body and heart weights (wt), mean MC volumes by Coulter Multisizer (vol), and cell protein content-per-MC by BCA assay (TotProtMC) were used to measure cardiac growth. A new approach using large-particle fluorescent activated cell sorting (FACS, see Figure) was validated to isolate 15K Troponin-T expressing ...
The effect of single-drug cis-diamminedichloroplatinum (CDDP) treatment on the distribution of T-lymphocyte subsets and monocytes was determined using monoclonal antibodies and a flow cytometry technique. Thirty-nine patients with radically operated ovarian carcinoma received postoperative treatment with six cycles of CDDP 50 mg/m2, and were examined either before, during, or after chemotherapy. During treatment, the number of OKT4+ (mainly T-helper) cells was reduced, whereas the number of OKT8+ (mainly T-suppressor/cytotoxic) cells was increased. Four to six months after the CDDP treatment was ended, these aberrations were no longer seen. The number of 1D5+ cells (monocytes) was not influenced by the treatment. It is concluded that no long-lasting change in the distribution of immunocompetent cells could be detected after this regimen of CDDP treatment.
The Aurora family of serine-threonine kinases are essential regulators of cell division in mammalian cells. Aurora-A and -B expression and kinase activity is elevated in a variety of human cancers and is associated with high proliferation rates and poor prognosis. AMG 900 is a highly potent and selective pan-aurora kinase inhibitor that has entered clinical evaluation in adult patients with advanced cancers. In mice, oral administration of AMG 900 blocks the phosphorylation of histone H3 on serine-10 (p-Histone H3), a proximal substrate of aurora-B and inhibits the growth of multiple human tumor xenografts, including multidrug-resistant models. In order to establish a preclinical pharmacokinetic-pharmacodynamic (PK-PD) relationship for AMG 900 that could be translated to the clinic, we used flow cytometry and laser scanning cytometry detection platforms to assess the effects on p-Histone H3 inhibition in terms of sensitivity, precision, and specificity, in human tumor xenografts in conjunction with
Written by Tim Bushnell, Ph.D. Flow cytometry is a powerful technique impacting both clinical and research.. If youre looking for a career, flow cytometry technology can take you many places. An experienced flow cytometrist can find a job in a biotechnology company, in academia, or in a clinical setting.. Rather than focusing on specific career information, I would like to highlight one career option that has been very good for me since my transition from research scientist to core manager over a decade ago.. Core facilities, or Shared Resource Laboratories (SRL) as this Cytometry A paper made popular, represent an investment by institutions in resources and personnel.. Staff and directors working in SRLs have the advanced training and experience to support the researchers and research mission of the institution.. Working in an SRL is a different and very exciting career option for researchers who enjoy flow cytometry, enjoy working on many different projects, and enjoy working in scientific ...
Summer Research Description: Dynein is an essential cytoskeletal motor protein, facilitating the transport of various materials within the cell by binding to and trafficking along microtubules. In non-dividing cells, dynein transports cargo from the cell periphery towards the nucleus and microtubule-organizing center, in a microtubule minus-end directed manner. Previous data indicates that dynein plays a role in HIV intracytoplasmic transport. In these studies, I used flow cytometry and X-gal analysis to assess the effects of Ciliobrevin, an inhibitor of dynein ATPase activity, on HIV infectivity. Using flow cytometry, I found that increasing the concentration of Ciliobrevin, gives an overall decrease in infectivity, however not to the extent shown by previous studies, likely due to differences in the approaches used to quantify the extent of infection. This increased inhibition of dynein leads to lowered infectivity because fewer HIV particles are being trafficked to the nucleus. I am also ...
Male mice model of degenerative liver was obtained through food fasting but still have drinking water for 5 days. It caused energy protein malnutrition and damage of liver tissue. The administration of 50% (v/v) honey was performed for 10 consecutive days, while the positive control group was fasted and not given honey and the negative control not fasted and without honey. Observations of regeneration the liver tissue based on histologically examination, observation of Hsp70 expression, and homing signal based on vascular endothelial growth factor-1 (VEGF-1) expression using immunohistochemistry technique. Observation on expression of CD34 and CD45 as the marker of auto mobilization of hematopoietic stem cells using flow cytometry technique ...
Flow cytometry; Cytofluorometry, Flow; Cytometry, Flow; Microfluorometry, Flow; Flow Microfluorimetry; Fluorescence-Activated Cell Sorting. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
I am a marine biologist and have worked at the International Research Institute of Stavanger (IRIS) for thirteen years. My main research interest is investigating effects of environmental changes on the physiology and behaviour of marine invertebrates. I have worked primarily with decapod crustaceans and bivalve molluscs, examining, for example, cardiac performance, immune function, valve gaping behaviour and expression of endogenous rhythms. A key component of this work is the development of methods that produce results readily interpreted within an ecological context. I am also involved in the development of flow cytometry techniques to measure blood cell parameters in samples taken from blue mussels. I have recently led projects tasked with developing monitoring techniques to detect oil discharges at sea, together with an investigation into potential environmental impacts arising from leakage of carbon dioxide from sub-sea storage. I am currently working on a project that aims to use the ...
The hallmark of an immune response to Mycobacterium tuberculosis is granuloma formation. CD4+Th1 cells are of central importance in anti-mycobacterial immunity by way of producing effector IFN-γ and TNF-α cytokines that help to contain infection in the granulomas by activating macrophages. The environment of the granuloma is hypoxic and we have little knowledge on CD4+ T cell processes and function in this environment. Autophagy is a de novo intracellular protein and organelle degradation pathway induced in eukaryotic cells by stress-factors such as starvation or hypoxia. It has been described that autophagy is induced in T cells upon activation and autophagic processes might contribute to nutrient supply and the regulation of cytokine production in T cells. The aim of this study was to investigate autophagic processes in CD4+ T cells after activation and the regulation of IFN-γ production under normoxia, and hypoxia as found in the granuloma of TB patients. The main assays, immunoblotting ...
Purpose:. Circulating tumor cell (CTC) enumeration or Androgen Receptor (AR) splice variant 7 expression from Epithelial Cell Adhesion Molecule (EpCAM) positive CTCs are predictive biomarkers for patients with prostate cancer. Recent reports suggest subpopulations of CTCs with decreased EpCAM expression may have greater invasive capacity or drug-resistant potential. These include cells with mesenchymal phenotypes that express N-cadherin, or MUC1, or stem cell populations that express CD133. Integrated molecular analysis across different CTC subpopulations has not been performed. We sought to compare the molecular profile of different populations of CTCs from the blood of patients with prostate cancer.. Methods:. A multiparametric flow cytometry assay was used to identify different populations of CTCs from patients with prostate cancer. We then employed an integrated CTC capture and analysis technology known as the VERSA (Versatile Exclusion-based Rare Sample Analysis) platform to in parallel or ...
Dec 09, 2020 (Heraldkeepers) -- The Global Flow Cytometry Market is segmented on the lines of its technology, component, application, end user and regional. Based on technology segmentation it covers bead based flow cytometry and cell based flow cytometry. Under component segmentation it covers instruments which further segmented into cytometry platforms, replaceable components, accessories, reagents and consumables, software and services. Based on application segmentation it covers academic and clinical research applications and diagnosis applications. Under end user it consists of commercial organizations, medical schools and clinical labs, hospitals, academics and others. The Global Flow Cytometry Market on geographic segmentation covers various regions such as North America, Europe, Asia Pacific, Latin America, Middle East and Africa. Each geography market is further segmented to provide market revenue for select countries such as the U.S., Canada, U.K. Germany, China, Japan, India, Brazil, ...
Product list of China Fcm Flow Cytometry System, show the variety of China products related to Fcm Flow Cytometry System; You can choose the right product of China Fcm Flow Cytometry System on this list.
This video demonstrates how to harvest cells from a spleen sample, and prepare a single cell suspension prior to performing cell isolation. Preparing a true single cell suspension of the primary tissue sample will optimize cell separation by avoiding addi
Background: CD34 a 110- to 115-Kda transmembrane sialoglyco pro- V twin, is expressed by early hematopoietic (myeloid and lymphoid) progenitor cells, endothelial cells, murine embryonic fibroblasts, and bone marrow (BM) stromal cells and their precursors. CD34 expression has great importance in diagnosis, classification and management of acute leukemia. Aim of the study: To evaluate the expression of CD34 (percentage and meanfluorescence intensity) in Sudanese patient with acuteleukemia. Materials and Methods: This is descriptive cross-sectional study, conducted in Flowcytometry centre in Khartoum state, 50 new cases of acute leukemia analyzed by flowcytometry for CD34expression. The flowcytometery lysing procedure for bone marrow aspiration and peripheral blood monoclonal antibody combination was done as the follows: All tubes were labeled then pipette into each tube 20 µL of CD34 monoclonal antibody and added 100 µL of sample containing no more than 1 x 104 leukocytes / ml. The tubes were ...
A procedure for enrichment in recombinant interleukin-2 (rIL2)-activated natural killer (NK) cells was developed and used for in vitro generation of antitumor effector cells from the peripheral blood of 20 patients with central nervous system (CNS) tumors. In comparison to the patients unseparated mononuclear cells and nonadherent lymphocytes cultured in the presence of 1000 U/ml of rIL2 for up to 3 weeks, interleukin-2-stimulated lymphoid cells, when purified by adherence to plastic, proliferated better (up to 6,720-fold expansion) and achieved up to five times higher levels of antitumor activity against K562 cell targets and NK-resistant glioblastoma cell targets. Two-color flow cytometry analysis showed that cultures of cells purified by adherence to plastic which had the best proliferation contained 10% or less of CD3+Leu19-T-lymphocytes, while the unseparated lymphokine-activated killer cell cultures which proliferated poorly contained up to 85% of CD3+Leu19-T-cells. Cultures of adherent ...
The aberrations of sperm DNA may cause various problems and have negative consequences on fertility. These influence embryonic development or might lead to early embryo loss. Sperm Chromatin Structure Assay (SCSA) is the flow cytometric method most often used for the detection of DNA lesions; however, some studies using that method reached confusing conclusions. The aim of this pilot study was to adjust and compare two alternative tests, namely the TUNEL test and the Nicoletti assay. The above-mentioned two flow cytometric methods capable of detecting the fragmented DNA of sperm were tested on 12 frozen-thawed stallion semen samples. The TUNEL test demonstrated much higher DNA fragmentation ratio than the Nicoletti assay (mean ± SD: 30.77 ± 13.03% vs. 1.93 ± 0.89%, respectively). A fluorescent microscopic check of the samples showed that TUNEL labelled the plasma membrane and the mitochondria in a nonspecific way, rather than detecting only the fragmented DNA, thus eventually resulting in a ...
Early, low-risk International Prognostic Scoring System (IPSS) myelodysplastic syndrome (MDS) is a heterogeneous disorder where the molecular and cellular hematopoietic defects are poorly understood. To gain insight into this condition, we analyzed gene expression profiles of marrow CD34+ progenitor cells from normal-karyotype, low-blast-count MDS patients, age-matched controls, and patients with non-MDS anemia. Given the heterogeneity of early MDS, a surprisingly consistent finding was decreased expression of B-cell lineage-affiliated genes in MDS patients compared with healthy controls and 3 of 5 samples with non-MDS anemia. Both patients with non-MDS anemia with reduced B-cell gene expression were on chemotherapy. In 25 of 27 of the original samples and 9 further MDS samples, Taqman real-time polymerase chain reaction (PCR) confirmed these data. Flow cytometry on unfractionated marrow from independent samples also demonstrated reduced B-cell progenitors in MDS patients compared with healthy controls.
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Flow Cytometry. Supports automated quality control, centralized data management and web-based data sharing. Integrates with ... LabKey Server provides a secure data repository for all types of biomedical data, including mass spectrometry, flow cytometry, ... Cytometry Part A. 73A (9): 847-56. doi:10.1002/cyto.a.20600. PMC 2591089. PMID 18615598. "The Best of Both Worlds: Integrating ... "Development of an automated analysis system for data from flow cytometric intracellular cytokine staining assays from clinical ...
"Flow Cytometry Intracellular Staining Guide". eBioscience, Inc. Retrieved 2011-09-25. "Croton Oil". OilHealthBenefits. ...
Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Lindmo T (eds), Alan R. Liss, Inc., New York. pp. 291-314. ISNBM 0-471- ...
... conjugated with phycoerythrin to label IgG or IgM-coated RBCs and enhance the detection by flow cytometry The flow cytometry is ... Flow cytometry is the method of choice. By examining markers on the surface of blood cells, the method can determine whether ... Due to their small size, PFCs are able to permeate circulation where erythrocytes may not flow. In tiny capillaries, PFCs ...
Advanced Flow Cytometry: Applications in Biological Research. Springer. p. 114. ISBN 9781402014840.CS1 maint: extra text: ...
Flow Cytometry and Cell Sorting (illustrated ed.). Springer Science & Business Media. 2000. p. 1072. ISBN 3540656308. Farmer, ...
For instance propidium iodide binds to DNA of dead cells and fluorescent signal is measured by flow cytometry. CDC assays are ... "Improved flow cytometry crossmatching in kidney transplantation". HLA. 92 (6): 375-383. doi:10.1111/tan.13403. ISSN 2059-2310. ... Live cells metabolise it into impermeable fluorescent product that can be detected by flow cytometry. This product can't be ... Besides CDC crossmatch there is flow-cytometric crossmatch available, that is more sensitive and can detect even complement non ...
Drug discovery High-throughput screening Drug discovery hit to lead Microscopy Flow cytometry Live single-cell imaging Haney SA ... Edwards BS, Oprea T, Prossnitz ER, Sklar LA (August 2004). "Flow cytometry for high-throughput, high-content screening". Curr ... High-content screening technology is mainly based on automated digital microscopy and flow cytometry, in combination with IT- ...
Offerings include preanalytical solutions for sample management; immunology research, including flow cytometry and multiomics ... BD FACSAria Cell Sorter-the first entirely new instrument in the next-generation portfolio of flow cytometers (2003), BD ...
Today, the gold standard is flow cytometry for CD55 and CD59 on white and red blood cells. Based on the levels of these cell ... PNH abnormalities on flow cytometry without signs of hemolysis. There are several groups where screening for PNH should be ... In patients with only a small clone and few problems, monitoring of the flow cytometry every six months gives information on ...
Fluorescence staining and flow cytometry for monitoring microbial cells. Journal of Immunological Methods 243: 191-210 UNSW: Dr ... of Oxytetracycline Production by Streptomyces rimosus in Soil Microcosms by Combining Whole-Cell Biosensors and Flow Cytometry ...
... and was involved in the early days of the development of flow cytometry. Even today, some flow cytometers utilize the Coulter ... the use of a flow cell lends itself to addition of a sheath flow, which keeps particles centered in the middle of the flow ... Hemocytometer Flow cytometry Hematology analyzer W. R. Hogg, W. Coulter; Apparatus and method for measuring a dividing particle ... In the flow cell format, this effect is minimized since sheath flow ensures each particle travels an almost identical path ...
"Key Features in the Latest Flow Cytometry Systems, Caitlin Smith, Biocompare". "Official website". Archived from the original ... The company offers solutions[buzzword] covering techniques of sample preparation, cell separation, cell sorting, flow cytometry ... Examples of these applications include sample preparation, cell separation, cell sorting, flow cytometry, molecular ... Miltenyi Biotec launches its MACSQuant Analyzer flow cytometry instrument 2011 - Miltenyi Biotec acquires Coley Pharmaceutical ...
Once hybridized, the microsphere beads are sorted, usually using flow cytometry. This allows for the detection of each of the ... For more details on this topic, see flow cytometry Since the optical identity of each microsphere is known, the quantification ... The recent reported high-throughput flow cytometry can sample a 96-well plate in 1 minute, and theoretically, the 100-plex ... Thus, the measurement by flow cytometry represents a replicate analysis of each array element. Effective gathering of ...
Flow Cytometry: Automation allows for analysis of thousands of cells per second. As with many kinds of viability assays, ... Flow cytometry Formazan-based assays (MTT/XTT) Green fluorescent protein Lactate dehydrogenase (LDH) Methyl violet Neutral red ...
FISH, PCR and flow cytometry experiments may be employed to confirm results. Array comparative genomic hybridization (also ... Cytometry 19:27-41. Shaw CJ, Stankiewicz P, Bien-Willner G, Bello SC, Shaw CA, Carrera M, Perez Jurado L, Estivill X, Lupski JR ...
"Data reduction for spectral clustering to analyze high throughput flow cytometry data". BMC Bioinformatics. 11: 403. doi: ...
... immunoblotting or flow cytometry can show absence of protein. Prenatal testing: It is particularly useful when a family member ... because the DHR signal yielded by flow cytometry depends on intact NADPH oxidase activity as well as the presence of a ... which can be measured by flow cytometry. This test is abnormal in patients with chronic granulomatous disease (there is no ... Rothe G, Oser A, Valet G (July 1988). "Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in ...
In the case of LAD-I, specific diagnosis is done by flow cytometry. This technique will reveal absent or reduced CD18 ...
This experimental setup is just one example of the capability of flow cytometry. In FACS systems, these characterized cells can ... Fluorescent Activated Cell Sorting, or FACS, utilizes flow cytometry to provide a fast, objective and quantitative measurement ... The flow path is changed in order to direct cells of particular interest to an outlet that traps the cells for further ... Other benefits include reduced risk of compromising the cell viability due to lowering the need for high pressure sheath flow ...
... flow cytometry), direct and bulk (biomass), indirect and individual (colony counting), or indirect and bulk (most probable ... "Cell cycle parameters of slowly growing Escherichia coli B/r studied by flow cytometry". J. Bacteriol. 154 (2): 656-62. PMC ...
... including flow cytometry. Two commonly used CD molecules are CD4 and CD8, which are, in general, used as markers for helper and ...
... the signal is measured using flow-cytometry. The sensitivity of flow-cytometry, eliminates the need for PCR amplification of ...
Howell-Jolly bodies can be identified and quantified using a blood smear or by flow cytometry. A high number of Howell-Jolly ... This ischemia can result in splenic sequestration, where large amounts of blood pool in the spleen but do not flow within ... Autosplenectomy can occur in cases of sickle-cell disease where the misshapen cells block blood flow to the spleen, causing ... This lack of blood flow can cause atrophy in the spleen and can lead to autosplenectomy.[citation needed] Pneumococcal sepsis, ...
The increasingly refined use of flow cytometry has also facilitated more accurate diagnosis of these lesions. Evidence is ...
As these cells are able to effectively efflux the dye, they can be detected via flow cytometry in what is termed the side ... Ashwood-Smith, M.J. (1994). "Safety of human sperm selection by flow cytometry". Human Reproduction. Oxford University Press. 9 ... Bisbenzimide Carcinogen DAPI DNA-binding protein Excited state Fluorescence Fluorescence microscopy Fluorophore Flow cytometry ... often with other fluorophores Flow cytometry to count or sort out cells. An example is the use of Hoechst dyes to analyse how ...
"The history and future of the fluorescence activated cell sorter and flow cytometry: A view from Stanford". Clinical Chemistry ... subscription required) Roederer, Mario (October 28, 2013). "Len Herzenberg - 1931-2013". Purdue University Cytometry ...
In the field of flow cytometry, a new technique uses ICP-MS to replace the traditional fluorochromes. Briefly, instead of ... A third flow (again usually around 1 liter per minute) of gas is introduced into the central tube of the torch. This gas flow ... Because elements are easily distinguished in ICP-MS, the problem of compensation in multiplex flow cytometry is effectively ... For both Laser Ablation and Desolvating Nebulisers, a small flow of Nitrogen may also be introduced into the Argon flow. ...
Scientists estimated the genome size for 40% of the species in the genus[6]; using Flow Cytometry. Penstemon digitalis has one ...
Flow cytometry bioinformatics. H. *Hypercycle (chemistry). I. *Inferring horizontal gene transfer. M. *Multi-state modeling of ...
Instead, plasma cells are identified through flow cytometry by their additional expression of CD138, CD78, the Interleukin-6 ...
Many modern molecular tests such as flow cytometry, polymerase chain reaction (PCR), immunohistochemistry, cytogenetics, gene ... blood flow, or other), and a radionuclide (usually either a gamma-emitter or a positron emitter). There is a degree of overlap ...
Flow-FISH[edit]. Flow-FISH uses flow cytometry to perform FISH automatically using per-cell fluorescence measurements. ... Microfluidics-assisted FISH (MA-FISH) uses a microfluidic flow to increase DNA hybridization efficiency, decreasing expensive ...
... flow cytometry). Additional biologically active molecules (such as antibodies) may also be attached to fluorescein, allowing ... Fluorescein is used as a rather conservative flow tracer in hydrological tracer tests to help in understanding of water flow of ... Fluorescein has often been used to track water movement in groundwater to study water flow and observe areas of contamination ... "Fluorescein Transport Assay to Assess Bulk Flow of Molecules Through the Hypocotyl in Arabidopsis thaliana". Bio-Protocol. 8 ...
An optimized method for routine HLA-B27 screening using flow cytometry. Cytometry, 1994; 18:21-29. K. Strauss, Insulin ... A comparative study of DNA content as measured by flow cytometry and image analysis in 1864 specimens. Analytical Cellular ... Cytometry. 1999 October 15;38(5):231-7. V. Chernyshov, E. Vykhovanets, I. Slukvin, Y. Antipkin, A. Vasyuk, K. Strauss, Analysis ... Cytometry, 1996; 26:52-59. B. Bertino, W. Knape, M. Pytlinska, K. Strauss, J-C. Hammou. ...
... cytometry, transmission electron microscopy, etc. have allowed scientists to get a better idea of the structure of cells.[8] ... proteins and lipids which are either free flowing or membrane bound, along with different internal compartments known as ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. This cell biology article is a stub. You can help Wikipedia ...
... where there is usually detectable protein levels by flow cytometry. The majority of the mutations causing classic WAS are ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. Retrieved from " ...
Institutional Flow Cytometry Core Facility. *Lions Sight Research Foundation. *MESA: Center for Health Disparities ...
Fluorescence can be measured by means of multi-well fluorometers or by flow cytometry methods. There are also enzymatic-based ... "Cytometry Part A. 79 (8): 603-612. doi:10.1002/cyto.a.21084. ISSN 1552-4930. PMC 3692008. PMID 21735545.. ...
Additional examination of the bone marrow by tests including flow cytometry and FISH are necessary to diagnose the specific ... These proteins can be stained with fluorescent dye labeled antibodies and detected using flow cytometry. The limit of detection ... Uses: Immunological methods are gaining wider use as more advanced flow cytometers are utilized for clinical testing. Patient ...
The preferred method of immunophenotyping is through flow cytometry. In the malignant lymphoblasts of ALL, expression of ...
Hutter, KJ (1982). "Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry". Histochemistry (75(3)): ... Torous, DK (2000). "Enumeration of micronucleated reticulocytes in rat peripheral blood: a flow cytometric study". Mutat Res ( ...
... and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in research applications.[5] ...
In the United States, forensic pathologists typically complete at least one year of additional training (a fellowship) after completing an anatomical pathology residency and having passed the "board" examination administered by The American Board of Pathology or The American Osteopathic Board of Pathology ("board-certified"). Becoming an anatomic pathologist in the United States requires completing a residency in anatomic pathology, which is on-the-job training one must perform upon completing medical school before one may practice unsupervised. Anatomic pathology (as it is called) by itself is a three-year residency. Most U.S. pathologists complete a combined residency in both anatomic and clinical pathology, which requires a total of four years. In the United States, all told, the education after high school is typically 13-15 years in duration (4 years undergraduate training + 4 years medical school + 4-5 years residency [anatomic and clinical pathology combined] + 1-2 year forensic pathology ...
Biochemical tests used in the identification of infectious agents include the detection of metabolic or enzymatic products characteristic of a particular infectious agent. Since bacteria ferment carbohydrates in patterns characteristic of their genus and species, the detection of fermentation products is commonly used in bacterial identification. Acids, alcohols and gases are usually detected in these tests when bacteria are grown in selective liquid or solid media. The isolation of enzymes from infected tissue can also provide the basis of a biochemical diagnosis of an infectious disease. For example, humans can make neither RNA replicases nor reverse transcriptase, and the presence of these enzymes are characteristic., of specific types of viral infections. The ability of the viral protein hemagglutinin to bind red blood cells together into a detectable matrix may also be characterized as a biochemical test for viral infection, although strictly speaking hemagglutinin is not an enzyme and has ...
A pathologist then examines the tumor and its stroma, perhaps utilizing staining, immunohistochemistry, flow cytometry, or ...
The deadliest diseases in humans are coronary artery disease (blood flow obstruction), followed by cerebrovascular disease and ...
Antibodies are used in flow cytometry to differentiate cell types by the proteins they express; different types of cell express ...
... phosphatidylserine exposure versus cell permeability by flow cytometry), cellular markers such as DNA fragmentation[70] (flow ... "A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry". ... "Cytometry. 27 (1): 1-20. doi:10.1002/(sici)1097-0320(19970101)27:1,1::aid-cyto2,;2-l. PMID 9000580.. ... Recent Advances in Cytometry, Part B - Advances in Applications. Methods in Cell Biology. 103. pp. 55-98. doi:10.1016/B978-0-12 ...
... flow cytometry). The report given by the pathologist is usually limited to a "benign" or "malignant" diagnosis, and ...
... subsets in term and significantly preterm UK infants in the first year of life analysed by single platform flow cytometry". ...
... uses fluorescence as a quantitative tool; the utmost sensitivity of flow cytometry is unmatched by other ... "Microfluidic impedance-based flow cytometry". Cytometry Part A. 77A (7): 648-666. doi:10.1002/cyto.a.20910.. ... Current Protocols in Cytometry, Wiley-Liss Pub. ISSN 1934-9297. *Flow Cytometry in Clinical Diagnosis, v4, (Carey, McCoy, and ... A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high ...
Disuse atrophy of muscles and bones, with loss of mass and strength, can occur after prolonged immobility, such as extended bedrest, or having a body part in a cast (living in darkness for the eye, bedridden for the legs etc.). This type of atrophy can usually be reversed with exercise unless severe. Astronauts in microgravity must exercise regularly to minimize atrophy of their limb muscles. There are many diseases and conditions which cause atrophy of muscle mass. For example, diseases such as cancer and AIDS induce a body wasting syndrome called cachexia, which is notable for the severe muscle atrophy seen. Other syndromes or conditions which can induce skeletal muscle atrophy are congestive heart failure and liver disease. During aging, there is a gradual decrease in the ability to maintain skeletal muscle function and mass. This condition is called sarcopenia, and may be distinct from atrophy in its pathophysiology. While the exact cause of sarcopenia is unknown, it may be induced by a ...
Blood flow is also affected by the smoothness of the vessels, resulting in either turbulent (chaotic) or laminar (smooth) flow ... The study of the blood flow is called hemodynamics. The study of the properties of the blood flow is called hemorheology. ... Thurston assembled this layer to the flow resistance to describe blood flow by means of a viscosity η(δ) and thickness δ from ... At any given pressure drop, the flow rate is determined by the resistance to the blood flow. In the arteries, with the absence ...
Vinogradov AE (1994). "Measurement by flow cytometry of genomic AT/GC ratio and genome size". Cytometry 16 (1): 34-40. PMID ...
Less fastidious, scalable, methods include the use of cytometers, while flow cytometry allows combining cell counts ('events') ...
March 2007). "Clinical value of anti-Leishmania (Leishmania) chagasi IgG titers detected by flow cytometry to distinguish ...
Since dense granules have surface membrane proteins, the activation of CD63 and LAMP-2 can be observed with flow cytometry. ... Another way to detect the secretion of dense granules is through flow cytometry. ...
Flow Cytometry. Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping. Flow ... cytometry is used for such varied applications as cell cycle analysis, telomere length determination, microvesicle analysis, ...
... uses flow cytometry Cell cycle analysis Coulter counter Cytometry Dielectrophoresis Flow Cytometry Standard Mass cytometry ... Media related to Flow cytometry at Wikimedia Commons Flow+cytometry at the US National Library of Medicine Medical Subject ... Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: ... Flow cytometry cell sorters have a collection system unlike flow cytometry analyzers. The collection process starts when a ...
... Recent technological innovations in flow cytometry now allow oceanographers to collect high-frequency ... Time-varying clusters in large-scale flow cytometry Jeremy Hyrkas. , Daniel Halperin. , Bill Howe. . IAAI Conference 2015 * ... Scalable clustering algorithms for continuous environmental flow cytometry Jeremy Hyrkas. , Sophie Clayton. , Francois Ribalet ... We propose the GMM with partitioning approach for classification of large-scale, high-frequency flow cytometry data. ...
Flow Cytometry. Technique using an instrument system for making, processing, and displaying one or more measurements on ... AnalyticalPhotometryLuminescent MeasurementsFluorometryCytophotometryFlow Cytometry. All MeSH CategoriesAnalytical, Diagnostic ... DiagnosisDiagnostic Techniques and ProceduresClinical Laboratory TechniquesCytological TechniquesCell SeparationFlow Cytometry ... and Equipment CategoryInvestigative TechniquesClinical Laboratory TechniquesCytological TechniquesCell SeparationFlow Cytometry ...
... *. Beckman Coulter Cell-Sorter (MoFlo XDP). Location: Department of Dermatology, Experimental Dermatology and ... It unites the possibility of high throughput data acquisition of flow cytometry with morphological information obtained by ... 10-colour flow cytometer, Gallios, Beckman Coulter. Location: Neurology, c/o ICB, Mendelstr. 7 Contact Person: Prof. Dr. ... The ImageStreamX is a functional combination of a flow cytometer and a fluorescence microscope. ...
English: Flow cytometry is a method in cell biology that employs the deflection of laser light a well as the excitation of ... flow cytometry technique of suspending cells in a stream of fluid and passing them by an electronic detection apparatus ... Media in category "Flow cytometry". The following 200 files are in this category, out of 349 total. ... Pages in category "Flow cytometry". This category contains only the following page. ...
Introduction to Flow Cytometry and FACSDiva 6.0 tutorials.. *Tools for developing a flow cytometry experiment including a ... The training class will cover the basics of flow cytometry and an introduction to the use of the instrument and software ... The UMass Flow Core Facility has been a great resource for our research on brain development. Aside from the high quality ... Training on the flow analysis equipment for new students/postdocs and research technicians will be conducted quarterly, an ...
The most important clinical application of flow cytometry is in hematologic malignancy diagnosis. ... The development of a specialized flow cytometer in the 1970s, triggered by the HIV pandemic, paved the way to a broad range of ... Flow cytometry is an invaluable tool used to analyze the chemical and physical properties of cells. This laboratory technique ... Role of antibodies in flow cytometry. Antibodies are an invaluable component of flow cytometry. The advent of monoclonal ...
You must make an appointment to utilize the staff-assisted machines. Please plan your experiments ahead and make sure the facility can accommodate your schedule. We recommend a minimum of 2 week advanced bookings. Please keep your appointments, and show up for them promptly. You will be billed for late cancellations.. For appointments and consultations, contact Facility Manager Rochelle Diamond, x3998 (afternoons), x4947 (message), at [email protected] A completed Biosafety Form must be submitted prior to requesting an appointment. ...
CA Flow Cytometry Website Flow cytometry measures and analyzes the characteristics of single particles, normally cells, as they ... Tens of thousands of cells can be interrogated by a flow cytometer in a single second. Flow cytometry can be used for a variety ... Thousands of cells can be analyzed by a flow cytometer in a single second. Among the measurements derived from flow cytometry ... The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps ...
Flow Cytometry. La Jolla, California Campus. The Flow Cytometry Core Facility is home to a variety of fluorescence-based ... Introduction to Flow Cytometry Web-Based Training] - BD Biosciences. \n*[Multicolor Flow Cytometry Resources] - BD Biosciences ... Practical Flow Cytometry] - Howard M. Shapiro, 2003. \n" }, { "title": "Flow Cytometry Organizations", "content": "\n*[ISAC - ... Current Protocols in Cytometry] - Scripps Research Network or valid login only. \n*[Cytometry Protocols] - Purdue University ...
KTH / Course web / Flow Cytometry Flow Cytometry Innehåll visas utifrån dina val. Om du inte hittar någon sida, schemahändelse ...
The introduction of smaller and easy-to-operate laser systems will further expand applications of flow cytometry to routine ... and suggests specific product and marketing opportunities for flow cytometry instruments and reagents.. Rationale. During the ... has announced the addition of the 2015 Strategies in the Global Flow Cytometry Market report to their offering. ... will have a profound impact on the flow cytometry markets worldwide. New molecular diagnostic and monoclonal antibody tests ...
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test. ...
The standard reference for anyone interested in understanding flow cytometry technology.. American Journal of Clinical ... Practical Flow Cytometry. Copyright © 2003 John Wiley & Sons, Inc. All rights reserved. ...
Flow Cytometry support is not available on University or bank holidays.. Within normal working hours. Liaise with the ... Flow Cytometry Core Facility. Newcastle University , Flow Cytometry Core Facility , Services. Top Services. Services. Overview ...
... Becton Dickinson Immune Function Laboratory. Location: Becton Dickinson Immune ... Our experienced staff is pleased to offer flow cytometry training as well as assisted and independent instrument use. Please ... Examples of services offered include flow cytometry, cell sorting, cytokine/chemokine analysis, and experimental design. ... To learn more about the Ross facility, visit the Flow Cytometry website. ...
This article describes how to prepare cells for flow cytometry, a popular technique used to analyze cell size, volume, and ... Then they can be centrifuged and resuspended in a flow cytometry staining buffer and used for flow cytometry. ... Flow cytometry is a popular technique used to measure the expression of molecules, identify cells, and analyze cell size and ... Single cells need to be produced from solid tissues for flow cytometry. This is done by disaggregating the tissue. Mechanical ...
... flow cytometry is forcing cells to pass detectors in single file. But it is also looking into cells more deeply than ever, ... GEN: Flow cytometry continues to evolve from its origins as a research tool. Outside the laboratory, where is flow cytometry ... Garret Guenther, Ph.D., Global Support Manager, Flow Cytometry, ACEA Biosciences. GEN: How is flow cytometry contributing to ... These activities, we have determined, are being facilitated by flow cytometry.. At Bio-Rad, we have incorporated flow cytometry ...
The Flow Cytometry Core provides investigators with state-of-the-art equipment and support for flow cytometric analysis and ... The Flow Cytometry Facility aims to provide investigators with state-of-the-art equipment and support for flow cytometric ...
The Core Facility Flow Cytometry is also involved in training and education of PhD-students and members of the DKFZ (see ... Head of Core Facility Flow Cytometry (FACS ). E-Mail: [email protected] Tel.:. +49 6221 42-1261 (Office). +49 ... Flow Cytometry is a powerful analysis tool in modern biology and life sciences. The applications are numerous and have ... The core facility flow cytometry is equipped with various instruments and software modules to analyse and sort your samples. ...
... part 1 This forms the first session of the Garvan Flow , Flow Cytometry Training course. this is a 1 1/2 day training course a… ... BASICS OF FLOW CYTOMETRY ? Measurement METRY Cells CYTO * 14. BASICS OF FLOW CYTOMETRY ? Measurement METRY Cells CYTO Flow Flow ... Flow Cytometry Measurement METRY Cells CYTO Flow Flow * 16. FLOW CELL Hydrodynamic focusing of sample to laser intercept - ( ... Flow Cytometry Training talks - part 1 This forms the first session of the Garvan Flow , Flow Cytometry Training course. this ...
Newcastle University , Flow Cytometry Core Facility , Core Technologies , Mass Cytometry. Top Mass Cytometry. Mass Cytometry. ... The technology is conceptually akin to traditional fluorescent flow cytometry except that antibodies and detection reagents are ... The Helios is the latest generation of the Cytof Mass Cytometer combining a flow cytometer with the power of a Time of Flight ... These systems combine the sample delivery and data output of a flow cytometer with the detection capabilities of a Time of ...
Flow cytometry. BrisSynBio has access to the equipment and expertise housed in the Flow Cytometry Facility. This comprises high ... Flow cytometry Equipment booking BrisSynBio users are welcome to book our equipment. ... Flow cytometry provides a technique for obtaining information about cells or other particles in a suitable buffer based on ... The Flow Cytometry Facility is located in Cellular and Molecular Medicine, in the Biomedical Sciences Building. ...
Analytical Flow Cytometry Flow cytometry is a fundamental tool used broadly in cell biological research. Protein expression or ... Flow Cytometry. The FCCIC provides assisted use services (hourly fee) for analytical flow cytometry on all instruments, ... Instrument training is mandatory for any user of the Flow Core who would perform unassisted flow cytometric analyses or sorts. ... The Flow Core recommends first time users contact Pam Whitney ([email protected]) to schedule a consultation before ...
... Richard R. Hardy hardy at Tue Jun 14 13:58:39 EST 1994 *Previous message: ... I need the advice of any other flow cytometry experts as well as those , versed in immunoflourescence. We are trying to do some ... Any thoughts would be appreciated! , If you have access to a flow cytometer with longer wavelength excitation (dye laser or ... there was a description of this technique back in the mid-80s in Cytometry, I think. All this assumes that we are talking about ...
Determination of lymphocyte division by flow cytometry.. Lyons AB1, Parish CR. ... and can resolve multiple successive generations using flow cytometry. The label is fluorescein derived, allowing monoclonal ...
Flow cytometry is a technology that measures and analyzes the optical properties of mono-dispersed single particles, such as ... The Bergen Flow Cytometry Core Facility is located on the 5th floor of the Haukeland University Hospital Laboratory Building at ... An important feature of flow cytometry is that large numbers, for example thousands of particles per second, are analyzed and ... However, training on the Aria cell sorter is limited to experienced flow cytometry users, users who will used the sorter ...
  • A flow cytometer allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second. (
  • A common variation involves linking the analytical capability of the flow cytometer to a sorting device, to physically separate and thereby purify particles of interest based on their optical properties. (
  • The first label-free high-frequency impedance flow cytometer based on a patented microfluidic "lab-on-chip", Ampha Z30, was introduced by Amphasys (2012). (
  • At the 5th American Engineering Foundation Conference on Automated Cytology in Pensacola (Florida) in 1976 - eight years after the introduction of the first fluorescence-based flow cytometer (1968) - it was agreed to commonly use the name "flow cytometry", a term that quickly became popular. (
  • A flow cytometer is similar to a microscope , except that, instead of producing an image of the cell, flow cytometry offers high-throughput, large-scale, automated quantification of specified optical parameters on a cell-by-cell basis. (
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (
  • The ImageStreamX is a functional combination of a flow cytometer and a fluorescence microscope. (
  • Amnis ImageStream Mark II Imaging Flow Cytometer -5 Color Analysis Capabilities 2 Excitation Lasers, 488nm and 633nm. (
  • The development of a specialized flow cytometer in the 1970s, triggered by the HIV pandemic, paved the way to a broad range of applications such as protein modifications studies, immunophenotyping, and analysis of intracellular antigens. (
  • A flow cytometer is a powerful piece of scientific equipment and the field of flow cytometry has provided researchers with data on the chemical and physical properties of cell and particle populations. (
  • A flow cytometer is similar to a microscope. (
  • Thousands of cells can be analyzed by a flow cytometer in a single second. (
  • In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. (
  • An event is either an actual biological cell or some other mass that was large enough to trigger the data acquisition capturing device of the flow cytometer instrument. (
  • These systems combine the sample delivery and data output of a flow cytometer with the detection capabilities of a Time of Flight mass spectrometer. (
  • New users will be trained by the core facility manager on the relevant flow cytometer. (
  • The course has been designed to allow the participant to gain experience of what a flow cytometer is capable and to raise awareness of the problems that can be encountered. (
  • The URMC Flow Cytometry Resource is grateful to NIH Office of Research Intfrastructure Programs for the funding of the CyTOF Mass Cytometer from the grant number 1 S10 OD012302-01. (
  • Total absolute cell count and percent lymphocyte values are usually obtained using the number of white blood cells and lymphocytes counted on an automated hematology instrument multiplied by the percentage of positivelystained cells measured on a flow cytometer. (
  • Beckman Coulter offers a variety of instrument setup, fluorospheres and quality control reagent products for optimum setup, daily maintenance and operation of your Beckman Coulter flow cytometer. (
  • Necessary reagents for the daily maintenance and operation of your Beckman Coulter flow cytometer. (
  • The SOEST Flow Cytometry Facility in the Pacific Ocean Sciences and Technology Building, Room 20 (POST 20) has two flow cytometers: one is a research-grade flow cytometer capable of sorting cells (Beckman-Coulter Altra) and the other is a bench top analyzer (Beckman-Coulter XL). (
  • In order to be qualified for running samples with no technical assistance on the Altra flow cytometer, the user must have spent a minimum of 2 hours technician-assisted run time for training and be deemed by the facility director to be qualified to use the instrument un-assisted. (
  • The XL flow cytometer can be used with more minimal training. (
  • With Amnis® charge-coupled device, time-delayed integration (CCD-TDI) technology , the CellStream® provides unparalleled high sensitivity in a highly customizable flow cytometer. (
  • The facility continues to burgeon with recent further investment permitting the purchase of the Accuri C6 Flow cytometer. (
  • The SH800 is a bench top high-speed multilaser flow cytometer and cell sorter, designed to be affordable and easy to use. (
  • Vancouver is the only centre to provide clinical Immunophenotyping using a thirteen colour Flow Cytometer. (
  • 2009 - Eight colour Flow Cytometer Canto II is in operation at BC Cancer. (
  • 2015 - Thirteen colour Flow Cytometer Fortessa is in operation at BC Cancer. (
  • The BD LSR II Flow Cytometer analyser can be used after training. (
  • The BD LSRFortessa Flow Cytometer analyser can be used after training. (
  • In this medical test, the sample is suspended in fluid which passes through a flow cytometer, a device designed to identify particles in a solution. (
  • Amnis imaging flow cytometers are available on two platforms: the FlowSight® Imaging Flow Cytometer, and the ImageStream® X Mark II Imaging Flow Cytometer. (
  • Part 1 (Introductory Training - a limited-time subscription to Excyte Master Class basic modules) serves to acquaint the users with the basics of how a flow cytometer works and, especially, what is going on inside "the box" when the user activates various functions. (
  • A basic understanding of how a flow cytometer works is critical to producing high quality data. (
  • Cell Signaling Technology (CST) offers a diverse portfolio of thoroughly validated flow cytometry antibodies in PBS formulation, suitable for use with the CyTOF ® mass cytometer. (
  • A Flow Cytometer comprises a fluidics, optics and electronics systems. (
  • Flow cytometer is a powerful single cell analysis tool that allows multi-parametric study of suspended cells. (
  • Hi, Soon we will test a demo flow cytometer. (
  • The company also launched a few innovative products in the past three years, such as ClearLLab LS lymphoid screen reagents, CytoFLEX Flow cytometer, and Aquios CL clinical flow cytometer. (
  • The beam may impinge a particle in a flow channel of a cytometer. (
  • 6. The system of claim 1 , wherein the target is a core stream of a flow stream channel of a cytometer. (
  • 14. The method of claim 13 , wherein the target is a core stream of a cytometer flow stream channel. (
  • Flow cytometer analyzes up to thousands of particles per second as the beam passes through the liquid stream. (
  • Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. (
  • Users of the LSRII will be able to sign up on a first come/first serve basis however you will not be able to sign up for more than 3 hours without prior permission from the flow facility manager, Amy Burnside . (
  • The UMass Flow Core Facility has been a great resource for our research on brain development. (
  • The Flow Cytometry Core Facility is home to a variety of fluorescence-based analytical and sorting cytometers, as well as a skilled staff who can offer services, training, insight, and support. (
  • To learn more about the Ross facility, visit the Flow Cytometry website . (
  • Welcome to the Ross Flow Cytometry Core Facility. (
  • The Flow Cytometry Facility aims to provide investigators with state-of-the-art equipment and support for flow cytometric analysis and high-speed cell sorting. (
  • The core facility flow cytometry is equipped with various instruments and software modules to analyse and sort your samples. (
  • The Core Facility Flow Cytometry is also involved in training and education of PhD-students and members of the DKFZ (see program Aus- und Weiterbildung). (
  • BrisSynBio has access to the equipment and expertise housed in the Flow Cytometry Facility. (
  • The Flow Cytometry Facility is located in Cellular and Molecular Medicine, in the Biomedical Sciences Building. (
  • The Bergen Flow Cytometry Core Facility is located on the 5th floor of the Haukeland University Hospital Laboratory Building at room 5160. (
  • After hour access to the facility and bench top flow cytometers may be granted for experiences users with the permission of the core facility manager. (
  • The core facility also offers courses in flow cytometry for master students. (
  • The Technology Facility labs are extremely well equipped for modern flow cytometry, including the two CyAn ADP analysers, with 3 lasers (405, 488 and 635nm) and 9 colour detectors. (
  • The Creighton Flow Cytometry Core Facility is now based in room 383 of the Criss III building on the Creighton campus. (
  • The Flow and Genomic Cytometry Core Facility provides comprehensive services and assistance in multi-parameter flow cytometry, FACS, single-cell genomic and transcriptomic analysis for internal and external users. (
  • The facility provides technical and scientific services and assistance in multi-parameter flow cytometry analysis , FACS (fluorescence activated cell sorting) and single-cell genomic and transcriptomic analysis. (
  • Flow cytometers currently available at the core facility allow for simultaneous detection of many fluorescent proteins of different fluorescent groups simultaneously expressed in the cells. (
  • The Flow Cytometry Facility operated out of the School for Ocean, Earth Sciences and Technology at the University of Hawaii provides state of the art instrumentation for analyzing and sorting particles. (
  • To facilitate the running of the facility the Schools of Biological Sciences and Medicine jointly funded a Flow Cytometry Technician Dr Andy Goldson. (
  • The UNC Flow Cytometry Core Facility (FlowCore) provides state-of-the-art flow cytometry services to the entire UNC-CH research community as well as to others in the Research Triangle Park area. (
  • We thank Robert Wersto and the NIA FC Core Facility for assistance with flow cytometry. (
  • The flow cytometry facility is located in NA2.302 and NA2.308. (
  • Shared by the Medical College of Wisconsin Cancer Center and Children's Research Institute, the Flow Cytometry Core is an advanced technology facility that assists investigators with evaluating cell populations based on surface and intracellular antigen expression, apoptosis, bead-based cytokine detection, and cell cycle analysis. (
  • A priority mission of the Flow Cytometry Core Facility is to educate users in properly designing flow cytometry experiments, operate analytic flow cytometers, and analyze flow cytometry data. (
  • The Medical School Flow Cytometry Core Facility is a resource available for all within the University, as well as undertaking work for external groups and comapnies. (
  • The flow cytometry core facility is delighted to announce that they have taken delivery of a new cell sorter, the BDFACSMelody. (
  • CBA Assays - Contact Flow Cytometry Core Facility for quotes. (
  • Hi All, Garvan Flow Cytometry Facility would like to thank all the designers who gave the time and creative input into this processs. (
  • Established in 1995, the Flow Cytometry Research Core Facility serves as an integral part of numerous research projects for investigators from multiple departments and disciplines at Saint Louis University. (
  • SLU's Flow Cytometry Research Core Facility is located in room 765 of the Doisy Research Center and is available from 8 a.m. to 5 p.m Monday through Friday. (
  • The Babraham Institute Flow Cytometry Core Facility offers high quality service and state-of-the-art instrumentation to members of Babraham Institute and external users. (
  • The Flow Cytometry Unit functions as a Shared Resource Laboratory (core facility) for the National Institute on Aging's (NIA) Intramural Research Program (IRP). (
  • This core facility provides analytical flow cytometry and cell sorting services as well as access to instrumentation which is essential to the biomedical research mission of the NIA IRP. (
  • The Flow Cytometry Core Facility (FCCF) provides flow cytometry analysis and cell sorting services. (
  • The flow cytometry core facility is a non-profit facility and is supported by grants from Helse Sør-Øst, OUS and UiO, as well as nominal user fees. (
  • More information can be found in the Flow Cytometry Core Facility web pages (OUS). (
  • St. Mary's Flow Cytometry Core Facility (FACS) is an Imperial College core facility available to all researchers. (
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (
  • Modern flow cytometers are able to analyze many thousand particles per second, in "real time," and, if configured as cell sorters, can actively separate and isolate particles at similar rates having specified optical properties. (
  • Recent technological innovations in flow cytometry now allow oceanographers to collect high-frequency flow cytometry data from particles in aquatic environments on a scale far surpassing conventional flow cytometers. (
  • The data streams produced by instruments such as SeaFlow challenge the traditional sample-by- sample approach in cytometric analysis and illuminate the need for scalable clustering algorithms to extract population information from these large-scale, high-frequency flow cytometers. (
  • These advances have the potential to be revolutionary for the technique as they have allowed flow cytometers to make sensitive, precise, specific analyses of nanoscale BPOs. (
  • Early flow cytometers were, in general, experimental devices, but technological advances have enabled widespread applications for use in a variety of both clinical and research purposes. (
  • As instruments have become smaller and easier to maneuver, flow cytometers have also come to be used for near-patient disease diagnosis. (
  • As flow cytometers become more capable-pushing the limits of color detection, for example-they allow us to collect vast amounts of information from small samples. (
  • From human cells to algae, bacteria and yeast, the flow cytometers provide analyses to identify and purify subsets of cells to study processes in the human body and in single-celled organisms. (
  • Select from the intuitive Guava ® easyCyte benchtop flow cytometers, the compact Muse ® personal cell analyzer, and the enabling Amnis ® imaging flow cytometers. (
  • Support reagents are grouped into several different categories based on their use and functionality with flow cytometers. (
  • Flow cytometers detect particles based on their size (scatter) and fluorescence (auto or imparted by a dye). (
  • Flow cytometers work ideally with cells in the 0.2 - 200 µm diameter size range and cell concentrations as low as 1000 cells/ml. (
  • These flow cytometers were purchased, in part, using funds from the National Science Foundation under MRI Grant No. 0215817. (
  • Data files generated on the flow cytometers are archived on compact disc. (
  • Agilent flow cytometers offer excellent capabilities, high quality data, and an easy to use platform to save researchers time when acquiring and analyzing data. (
  • Luminex flow cytometers and cellular analysis instruments give you instant access to all facets of cellular phenotypes and morphology. (
  • The FlowSight® and ImageStream® Imaging Flow Cytometers add quantitative, microscopy imaging of every cell to your flow cytometry. (
  • At this moment, Biocenter Flow Cytometry unit has two flow cytometers, BD LSR II and BD LSRFortessa. (
  • Most commercial flow cytometers available today are bulky, expensive instruments requiring high maintenance costs and specially trained personnel for operation. (
  • Unlike the previously reported micro-flow cytometers, the developed system relies entirely on the microchannel geometry for particle focusing, eliminating the need for complex microchannel designs and additional microfluidic plumbing associated with sheath-based techniques. (
  • Data standards include the widely adopted Flow Cytometry Standard (FCS) defining how data from cytometers should be stored, but also several new standards under development by the International Society for Advancement of Cytometry (ISAC) to aid in storing more detailed information about experimental design and analytical steps. (
  • Flow cytometers operate by hydrodynamically focusing suspended cells so that they separate from each other within a fluid stream. (
  • In addition to the five available flow cytometers, the core oversees multiple ancillary instruments for use with sample preparation or further analysis. (
  • Powered with five high-performance flow cytometers, as well as Bio-Rad Bioplex 200 and MSD platereaders, we can help you design and analyse samples for extracellular or intracellular markers, proteins and cytokines, DNA content, and cellular processes such as calcium flux and membrane potential. (
  • Cytek Northern Lights is upgradeable from one laser (9 colors) to three lasers (24+ colors) and is priced more competitively than conventional three-laser flow cytometers, while offering an intuitive workflow and lowering the total cost of ownership. (
  • There is a pressing need for increased access to intuitive, high-capability flow cytometers and the deep biological insights that they bring. (
  • Northern Lights can expand with application requirements and can be upgraded from one laser (up to nine colors) to three lasers (beyond 24 colors), all while being priced more competitively than conventional three-laser flow cytometers. (
  • While conventional flow cytometers typically require five lasers, some of which are expensive and high maintenance, to achieve 18+ colors, Northern Lights enables the power of 24+ colors with just three relatively low-maintenance lasers. (
  • Abstract Agave BioSystems proposes to develop and demonstrate an innovative Organic Thin Film Transistor (OTFT) to detect cell characteristics without the need for cell labeling for use in microfluidic flow cytometers. (
  • flow cytometers does anybody knowhow to make a DIY sheath fluid? (
  • We encourage you to contact us in the early planning stages of mass cytometry experiments. (
  • Advanced courses and a symposium about new developments in flow and mass cytometry are organized to update users and others with an interest in these topics and to stimulate interaction between users. (
  • CyTOF ® mass cytometry single cell proteomics platform uses metal conjugated antibodies to simultaneously profile over 30 proteins within a single cell. (
  • if you have issues creating a Mass Cytometry iLab account. (
  • Starting January 3, users can use the new iLab Mass Cytometry system to book appointments online. (
  • Existing mass cytometry users will be migrated into the system. (
  • Note: The new booking system only impacts mass cytometry services. (
  • RchyOptimyx can assess and graphically present the trade-offs between marker choice and population specificity in high-dimensional flow or mass cytometry datasets. (
  • and suggests specific product and marketing opportunities for flow cytometry instruments and reagents. (
  • As flow cytometry is increasingly used in new applications, particularly nontraditional immunology workflows, the technique must become less complex, and this will happen by making instruments smarter and preconfiguring reagents. (
  • The technology is conceptually akin to traditional fluorescent flow cytometry except that antibodies and detection reagents are tagged with stable isotopes of rare earth metals instead of fluorochromes. (
  • Comprehensive QC solutions and versatile support reagents for your flow cytometry analysis. (
  • Optimal flow cytometry data using our reagents for instrument setup, quality control and absolute counting. (
  • Research and Markets ( ) has announced the addition of the '2015 Strategies in the Global Flow Cytometry Market' report to their offering. (
  • The global flow cytometry market is expected to reach USD 4.79 Billion by 2022 from USD 3.29 Billion in 2017 growing at a CAGR of 7.8% during forecast period. (
  • Becton, Dickinson and Company (US) held the leading position in the global flow cytometry market in 2016. (
  • The company is focused on strengthening its market position in the global flow cytometry market mainly by adopting the strategies such as product launches, agreements, collaboration, and acquisitions. (
  • It unites the possibility of high throughput data acquisition of flow cytometry with morphological information obtained by microscopy. (
  • Compatible with autosampling and high-throughput technology, sensitive to a rainbow of colors, capable of processing multiple inputs in parallel, and reconcilable with activities upstream and downstream (including PCR analysis and genome sequencing), flow cytometry is helping advance single-cell analysis. (
  • Given their high-throughput capacity for detecting and quantifying analytes in solution, and given their multiplexing potential, flow cytometry systems will be quickly adapted to diverse research and industrial sectors. (
  • Flow cytometry, utilizing antibodies recognizing targets restricted to the hPSC surfaceome, offers an invaluable tool for high-throughput validation of hPSC lines. (
  • Inertial microfluidics for sheath-less high-throughput flow cytometry. (
  • Finally, diagnosis using flow cytometry data can be aided by supervised learning techniques, and discovery of new cell types of biological importance by high-throughput statistical methods, as part of pipelines incorporating all of the aforementioned methods. (
  • Founded by Dr. Ryan Brinkman, a leader in flow cytometry informatics, a Distinguished Scientist at the British Columbia Cancer Agency, and Professor of Medical Genetics at the University of British Columbia, Cytapex removes the barriers to high-throughput and high-dimensional cytometry data by providing expert services in data management, quality checking, and data analysis, including customized gating and biomarker discovery pipelines. (
  • The most important clinical application of flow cytometry is in hematologic malignancy diagnosis. (
  • In the past, both normal hematopoietic tissue and leukemias have been the tissue samples of choice in the application of flow cytometry, and some of the most recent applications with these tissues are presented here. (
  • The chapter of immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. (
  • [2] The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde from the University of Münster , filed for patent on 18 December 1968 [3] and first commercialized in 1968/69 by German developer and manufacturer Partec through Phywe AG in Göttingen . (
  • [4] Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (
  • Impulszytophotometrie ), based on the first patent application on fluorescence-based flow cytometry. (
  • Among the measurements derived from flow cytometry are the size, relative fluorescence and complexity of the particle. (
  • The cells flow single file past focused laser beams and light scatter or fluorescence data is collected. (
  • PnB] Flow cytometry data is typically saved for analysis in the form of an array, with fluorescence and scatter channels represented in columns, and individual "events" (most of which are cells) forming the rows. (
  • Flow cytometry provides a technique for obtaining information about cells or other particles in a suitable buffer based on laser light scatter and fluorescence emission by bound fluorochromes. (
  • Compensation in Flow Cytometry: When the emission spectra of two or more fluorochromes overlap, the fluorescence from those fluorochromes may be detected on a given photomultiplier (photodetector). (
  • Antibodies are an invaluable component of flow cytometry. (
  • The advent of monoclonal antibodies in 1977 promised an unlimited supply of highly specific antibodies and dramatically changed the flow cytometry technique. (
  • The development of monoclonal antibodies against specific phosphoepitopes that can help in detection of protein activation states have enabled the use of flow cytometry to study cellular function. (
  • Also, high concentrations of antibodies in flow cytometry can lead to non-specific binding which will complicate the process. (
  • Therefore, these aggregates and polymers need to be removed from antibodies before using them in flow cytometry. (
  • Sigma® Life Science, the Life Science division of Sigma-Aldrich, provides monoclonal antibodies validated specifically for flow cytometry in pure form or conjugated to some commonly used fluorochromes. (
  • Thermo Fisher Scientific offers antibodies conjugated to 24 different fluorescent dyes and proteins for use in flow cytometry. (
  • During the next five years, continued advances in molecular diagnostics, monoclonal antibodies, lasers and IT, as well as growing understanding of immunologic forces regulating systemic diseases, will have a profound impact on the flow cytometry markets worldwide. (
  • This article reviews technical aspects of flow cytometry, availability of antibodies suitable for studies in domestic animals, and applications in veterinary oncology with emphasis on characterization of round cell tumors. (
  • Here we describe the immunophenotyping of live human embryonic stem cell (hESC, H9) and human induced pluripotent stem cell (hiPSC, KB3) lines by flow cytometry using a panel of antibodies identified as either stem cell reference markers (CD90, EpCam) or reported as being prevalent or restricted (c-Kit, HPI-1, Integrin α6, Semaphorin-6A) to these cells. (
  • Choose from hundreds of flow cytometry validated CST™ antibodies. (
  • Flow cytometric analysis, with monoclonal antibodies, has so far been restricted to leukocyte populations, which had been separated from erythrocytes before staining and/or analysis. (
  • We highly encourage you to contact the flow core for help with multi-color panel design before purchasing antibodies and for advice on sorting preparation. (
  • Flow cytometry is used for such varied applications as cell cycle analysis, telomere length determination , microvesicle analysis , receptor occupancy, phagocytosis and many other studies. (
  • Training on the flow analysis equipment for new students/postdocs and research technicians will be conducted quarterly, an email will be sent to the flow-core-l email list and the schedule will be posted. (
  • The training class will cover the basics of flow cytometry and an introduction to the use of the instrument and software analysis of data. (
  • In flow cytometric analysis, suspension of a single cell or particle is prepared and aspirated into a flow cell. (
  • Nanoscale flow cytometry is a new technology that has provided flow cytometric analysis at the nanoscale. (
  • Flow cytometry can be used for a variety of applications, most commonly including cell analysis or high speed cell sorting. (
  • Examples of services offered include flow cytometry, cell sorting, cytokine/chemokine analysis, and experimental design. (
  • Flow cytometry is an incredibly powerful tool for single-cell analysis because it lets labs quickly interrogate millions of cells. (
  • More recently, flow cytometry has been used to improve the analysis of small particles such as circulating exosomes, which promise to inform liquid biopsies. (
  • Now, flow cytometry is heading into single-cell analysis. (
  • We expect that in the near future, more scientists will either conduct analysis of single cells, in both biological and clinical samples, or verify processes by flow. (
  • The core offers state-of-the-art flow cytometry cell analysis and high-speed sorting to the research community at Hopkins. (
  • Serveral flow cytometric cell sorters (up to 5 laser, cuvette and jet-in-air sorter) enable the analysis of up to 19 parameters per cell. (
  • Flow Cytometry is a powerful analysis tool in modern biology and life sciences. (
  • In addition to this key instrument, our FACSAria TM instruments can also be used for more routine flow analysis applications. (
  • Quickly get meaningful results from our cytometry platforms that combine simplified sample preparation with powerful data analysis. (
  • and third, because the analysis of the acquired data requires an extensive expertise on flow cytometry to accurately detect the SP events. (
  • Flow cytometry is a highly sensitive and specific method for simultaneous analysis of multiple parameters of individual cells in a suspension. (
  • It can yield more information from each sample and rare event analysis is more accurate and efficient because of the increased flow rate and the digital acquisition system. (
  • Most commonly used in the context of Immunology, we have also started to see the adaption of flow cytometry for environmental studies, extracellular vesicle analysis, and the ability to use upwards of 30+ different parameters for more comprehensive analysis. (
  • Flow Cytometry facilitates cell and particulate analysis allowing assessment of new medicines and their efficacy, assessment of new delivery mechanisms (nanotechnology), product validation and testing of health claims. (
  • Guava® and Muse® flow cytometry kits are designed for the analysis of cellular events and/or cell phenotypes. (
  • Intracellular flow cytometry offers unique advantages to the study of signaling events when compared to western blot analysis. (
  • When doing the same analysis by flow cytometry, co-staining with CD19 and CD4, B and T cell-specific markers, respectively, can identify B cells. (
  • The rapid growth in the multidimensionality and throughput of flow cytometry data, particularly in the 2000s, has led to the creation of a variety of computational analysis methods, data standards, and public databases for the sharing of results. (
  • The Flow Cytometry Lysing Solution can be used with or without sample washing and is suitable for the analysis of normal and malignant leukocyte populations derived from various human biological samples (blood, bone marrow and others) using Flow Cytometry. (
  • With Abcam's Flow Cytometry Lysing Solution the analysis of whole blood by flow cytometry has become as easy and accurate as the analysis of separated cell populations. (
  • Learn researchers how to analyze flow cytometry data in an efficient way using FlowJo v10,the world's nr.1 analysis software tool for flow cytometry data. (
  • The use of flow cytometry can be divided into two broad categories, cell analysis and cell sorting. (
  • Submit samples for flow cytometry analysis by placing them in the designated sample refrigerator just inside the flow core doors. (
  • You can have your files uploaded to an external memory device to take back to your own lab for data analysis or you may request that the flow core staff analyze the data for you. (
  • All cytometry services are performed at an hourly rate, including the acquisition of samples and analysis. (
  • Combining analysis of surface markers and intracellular signaling readouts through multiplexed flow cytometry adds power to your experiments. (
  • For those who have not used the technique, this seminar will provide background, theory and applications of flow cytometry to the analysis of drinking water. (
  • Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. (
  • Strategic analysis of the market developments between 2014 and 2017 revealed that several growth strategies such as product launches, enhancements, acquisitions, partnerships, collaborations, and expansions were adopted by the market players to strengthen their product portfolios and maintain a competitive position in the flow cytometry market. (
  • The company offers a comprehensive range of solutions for flow cytometry-based cell analysis and cell sorting. (
  • Analysis of high-dimensional flow cytometry datasets can reveal novel cell populations with poorly understood biology. (
  • We offer a range of services, including sample analysis and cell sorting, as well as instrumentation, software, and technical expertise/advice for a variety of flow cytometric protocols. (
  • We also provide training and assistance for flow cytometry analysis of samples as well as technical expertise and advice for experimental planning and design, cell preparation, analysis and data handling. (
  • Under the partnership, the companies will offer consulting services in flow cytometry data analysis. (
  • Together with FlowJo ® , the leading single-cell flow cytometry analysis software, the partnership aims to put the most powerful bioinformatics approaches into the hands of anyone who needs them, in a manner that is familiar and friendly to use. (
  • I knew over 10 years ago when we started developing automated analysis tools for flow cytometry we were never going to come close to being able to deliver the ease of use that users gain from FlowJo. (
  • Based on technology developed at Stanford, the company was founded in 1997 and provides the leading analysis platform for single-cell flow cytometry analysis. (
  • Flow Cytometry Analysis of. (
  • He pioneered the use of flow cytometry and sorting in plants, developing widely-used methods for the analysis of genome size and cell cycle status, and for the production of somatic hybrids. (
  • He also was amongst the first to develop methods for the analysis of gene expression within specific cell types, using markers based on Fluorescent Protein expression for flow sorting these cells, and microarray platforms for analysis of their transcriptional activities and protein complements. (
  • Current projects in his lab include spectral flow cytometry, single particle analysis of natural and synthetic nanoparticles, and surface enhanced Raman scattering (SERS) in cytometry. (
  • The Cyto-Comp Cell Kit provides lyophilized human lymphocytes that are used to establish and monitor color compensation where multi-color flow cytometry assays are performed. (
  • NEW YORK (GenomeWeb News) - Beckman Coulter Life Sciences announced late Thursday it has acquired flow cytometry firm Blue Ocean Biomedical for an undisclosed amount. (
  • Beckman Coulter (US) is another major player in the flow cytometry market in 2016. (
  • Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers , but has many other applications in basic research, clinical practice and clinical trials . (
  • The introduction of smaller and easy-to-operate laser systems will further expand applications of flow cytometry to routine clinical laboratories. (
  • Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. (
  • Clinical uses of flow cytometry, which have exploded in recent decades, include validating hematopoietic stem cells pretransplantation and identifying irregularities in immune cell subsets that are present in cancer and immunodeficiency disorders. (
  • Since its inception, flow cytometry has been important in clinical diagnosis. (
  • The URMC Flow Core sincerely thanks the University of Rochester Clinical & Translational Science Institute's (Grant number UL1 RR024160) for their considerable and continued support of mission critical equipment. (
  • F. Venet, A. Lepape, and G. Monneret, "Clinical review: flow cytometry perspectives in the ICU-from diagnosis of infection to monitoring of injury-induced immune dysfunctions," Critical Care , vol. 15, no. 5, p. 231, 2011. (
  • 1980 - Explosive growth in the use of Flow Cytometry in clinical medicine. (
  • This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors institution. (
  • Two case studies will be presented to detail the unique aspects of conducting flow cytometry in both a preclinical and clinical regulated setting. (
  • Factors such as continuous technological advancements in the field of flow cytometry, prevalence of target diseases (such as HIV/AIDS and cancer), growing adoption of flow cytometry in advanced research activities and clinical trials, and the increasing availability of novel application-specific flow cytometry products are driving the growth of the flow cytometry market. (
  • How attractive is the clinical markets for flow cytometry? (
  • The clinical testing laboratories segment is expected to grow at a rapid rate in the near future owing to the increase in prevalence of the various diseases in which flow cytometry is required for diagnosis. (
  • The Working Group will promote and encourage applications of flow cytometry in diagnostics and clinical research through publication of educational material and the organisation of courses and symposia. (
  • He is currently the director of the Purdue University Cytometry Laboratories at Purdue University. (
  • Please do not hesitate to contact us if you have any questions about potential uses of flow cytometry for your research projects, how to appropriately design experiments for flow cytometry, prepare samples, or how to analyze your data. (
  • Flow Cytometry Standard (FCS) is a data file standard for the reading and writing of data from flow cytometry experiments. (
  • See our new page on Rigor and Reproducibility Guidelines for Flow Cytometry Experiments. (
  • Please do not hesitate to contact us if you have any questions about flow cytometry, if you want to know if you can use it in your research, how to design experiments, prepare samples, or how to analyze your data. (
  • Training for other aspects of conducting flow cytometry experiments will be undertaken in individual discussions with users. (
  • Our goal is for you to be successful with your flow cytometry experiments. (
  • Thus combined with the detection of cell surface markers intracellular flow cytometry has evolved into a powerful platform, which enables characterization of signaling networks at a single cell level within phenotypically distinct cell populations. (
  • Here, we provide a variety of protocols to aid in the preparation of cells for flow cytometry (how to do a proper antibody titration, general procedures to stain extracellular as well as intracellular antigens, etc. (
  • However, conventional flow cytometry does not have the degree of sensitivity necessary for the study of nanoscale biological particles and organelles (BPOs). (
  • Flow cytometry measures and analyzes the characteristics of single particles, normally cells, as they move in a stream and are passed through a laser. (
  • Flow cytometry measures phenotypic, biochemical and molecular characteristics of individual cells or particles suspended in a fluid stream. (
  • Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. (
  • This is performed using laminar flow to centre the suspension of single cells or particles as they pass through the laser beam(s). (
  • Flow cytometry is a technology that measures and analyzes the optical properties of mono-dispersed single particles, such as cells, bacteria, picoplankton, microbeads, yeast, platelets, nuclei and other similarly-sized particles, passing single file through a focused laser beam. (
  • An important feature of flow cytometry is that large numbers, for example thousands of particles per second, are analyzed and therefore provide a statistically significant picture of a specimen's physical and biochemical make-up. (
  • Flow cytometry is a powerful technique used to quantitively measure individual cells and other particles in suspension, at the rate of thousands of cells per second. (
  • The point at which the particles are delivered into the sheath fluid is commonly termed the Flow Cell. (
  • The design takes advantage of the Dean drag and inertial lift forces acting on particles flowing through a spiral microchannel to focus them in 3-D at a single position across the microchannel cross-section. (
  • Flow cytometry, typically using fluorescent probes that bind to specific cell-associated molecules, allows measurements of various phenotypic, biochemical and molecular characteristics of individual cells (or particles) suspended in a fluid stream. (
  • A flow cytometry apparatus for determining one or more characteristics of particles or the like flowing in a liquid stream includes a nozzle for generating a liquid flow stream for moving particles therethrough substantially one at a time. (
  • Flow cytometry analyzes the whole blood component, i.e., physical and chemical characteristics of the particles present in the blood. (
  • This allows more flexibility in experiment design and expands the detectable parameters range of flow cytometry. (
  • The focus of this symposium was on the present and future capabilities of flow cytometry for both medical and biological applications in cancer. (
  • At Cytek, we are committed to advancing the scope, reach and capabilities of flow cytometry, and the only way to accomplish this mission is by developing systems that are powerful and affordable," noted Dr. Wenbin Jiang, CEO of Cytek Biosciences. (
  • Uses for flow cytometry include: Cell counting Cell sorting Determining cell characteristics and function Detecting microorganisms Biomarker detection Protein engineering detection Diagnosis of health disorders such as blood cancers A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. (
  • In its present configuration, the analyzer is capable of performing twenty parameters (forward and side scatter plus eighteen color flow cytometry). (
  • In fact, rapidly growing areas of research - such as CAR-T cell therapy and immuno-oncology - are driving the flow cytometry analyzer market, which is expected to grow to more than $1 billion dollars by 2020. (
  • The URMC Flow Core would also like to thank the Rochester Human Immunology Center for their support of mission critical equipment. (
  • The results of these measurements correlated well with those obtained using the virtual flow cytometry technique, suggesting that hemato-pathologists could confidently use the latter technique for diagnosis or prognosis when only tissue immunohistochemistry is available - that is, when fresh sample is not. (
  • Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous steps. (
  • In biotechnology , flow cytometry is a laser - or impedance -based, biophysical technology employed in cell counting , cell sorting , biomarker detection and protein engineering , by suspending cells in a stream of fluid and passing them through an electronic detection apparatus. (
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (
  • Flow cytometry is a method in cell biology that employs the deflection of laser light a well as the excitation of fluorescent dyes to analyse various properties of a high number cells in a relatively short time. (
  • Flow cytometry is an invaluable tool used to analyze the chemical and physical properties of cells. (
  • However, instead of producing an image, flow cytometry provides automated quantification of optical parameters of cells and cell populations. (
  • Flow cytometry has been used extensively to study eukaryotic cells and is an incredibly versatile technique. (
  • The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. (
  • Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties. (
  • Long capable of running phenotypic screens, flow cytometry is starting to apply its powers of discrimination to genetically modified cells. (
  • Our experts emphasize what may be the most curious thing about flow cytometry: Although it forces cells to pass in single file, it also allows cell biology to spread out in many directions. (
  • The technique is applicable to in vitro cell division, as well as in vivo division of adoptively transferred cells, and can resolve multiple successive generations using flow cytometry. (
  • Traditionally, flow cytometry has been used to identify distinct cell types within a heterogeneous pool of cells, based on extracellular or surface marker expression, an application commonly known as immuno-phenotyping. (
  • Flow cytometry plays a significant role in the understanding of many biological processes, due to its ability to simultaneously analyse multiple parameters on individual cells. (
  • Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells . (
  • The CHEMICON APO-BRDU Kit is a two color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. (
  • As the cells flow past a focused laser beam of appropriate wavelength, probes fluoresce and emitted light is collected and directed to appropriate detectors. (
  • Globally, flow cytometry remains a popular tool for research applications including immuno-phenotyping, cell proliferation, cancer, and stem cells. (
  • The ability to measure multiple parameters of individual cells is possible using Multicolor Flow Cytometry ( see figure ). (
  • The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps on a fee-for-service basis. (
  • this is a 1 1/2 day training course aimed at giving new and experienced researchers a better understanding of cytometry in medical and biological research. (
  • The software developed by the researchers generates a two-parameter dot-plot display, similar to those found in flow cytometry, offering the objectivity of the latter method but with tools generally available to hematopathologists and other clinicians. (
  • Researchers have developed a method for converting immunohistochemistry data into objective, flow cytometrylike information. (
  • We provide a flow cytometry service to medical researchers in sydney australia. (
  • The core staff is here to help all researchers, whether novice or expert in flow cytometry, understand how this powerful technology can benefit your research endeavors. (
  • The services complement researchers' work in flow cytometry and allows for custom pipeline development, including plugin "app" development for the FlowJo application. (
  • Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations. (
  • Most commercial flow cytometry equipment has a lower size limit in the range of 100-300 nm based on the detection of beads. (
  • Not only can we use flow cytometry with associated fluorescent markers to understand the biology of cancer, but also the wide array of existing and developing markers provides us with important diagnostic tools in the detection of cancer early in either the malignant or relapse process. (
  • An optical detection system for flow cytometry that uses two or more light sources positioned laterally at different distances from the central axis of the flow stream for providing light through different parts of the flow stream. (
  • Flow cytometry is a laser-based biophysical technology involved in the cell sorting, cell counting, protein detection, and biomarker detection. (
  • Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping. (
  • Your search - 'Flow cytometry' - did not match any resources. (
  • Lessened spill over compared to fluorescent cytometry. (
  • Phagocytosis and postphagocytic reaction of cord blood and adult blood monocyte after infection with green fluorescent protein-labeled Escherichia coli and group B Streptococci," Cytometry B , vol. 76, no. 4, pp. 271-284, 2009. (
  • Flow cytometry is a technique used for measuring and analysing certain characteristics of a single particle or cell. (
  • Flow cytometry is also finding new uses in familiar settings, such as the research laboratory and the biopharmaceutical production line. (
  • Outside the laboratory, where is flow cytometry making significant contributions? (
  • Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. (
  • Flow cytometry is validating transfection, confirming whether desirable edits have been achieved, measuring the functional effects of gene editing, and enriching cell populations based on functional cell sorting. (
  • Additionally, it is also equipped with BD FACS flow supply systems to automate fluid handling. (
  • The FCF provides a beginners course about the basics of flow cytometry for new users. (
  • The result is a flow cytometry readout with information about whether given proteins are present, and their numbers. (
  • If appropriate, a doctor may recommend platelet flow cytometry for this ongoing monitoring, in the interest of keeping a close eye on platelet counts and specific proteins in the blood. (
  • We explore how available algorithms commonly used for medical applications perform at classification of such a large-scale, environmental flow cytometry data. (
  • These and other nontraditional applications are highlighted in this article, which reflects the views of three experts from leading providers of flow cytometry technology. (
  • Additionally, scientists are adapting instruments for nontraditional flow cytometry applications, from biomanufacturing and bioprocessing monitoring (including water quality testing and agricultural and food safety certification) to veterinary medicine, oceanography, and ecological field research. (
  • The course will initially cover basic flow cytometry but then rapidly move on to the more advanced applications. (
  • Headed by Dr Rachael Walker, the knowledgeable flow cytometry team have a combined cytometry experience of over 30 years and extensive knowledge in a variety of flow cytometry applications. (
  • The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology. (
  • A big 'plus' is the compilation of well-written protocols for many common flow cytometry based applications in a single conveniently sized and affordable book. (
  • This approach outperforms current state-of-the-art cytometry classification algorithms in accuracy, and can be coupled with manual or automatic partitioning of data into homogeneous sections for further classification gains. (
  • We propose the GMM with partitioning approach for classification of large-scale, high-frequency flow cytometry data. (
  • Flow cytometry is a method that has the capability to analyze nanoparticles and provide relevant data for scientists in the field. (
  • As reported in the Jan. 15 issue of Cytometry Part B , the method converts immunohistochemistry data to a two-parameter dot-plot display, much like those found in flow cytometry. (
  • Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing, and analyzing flow cytometry data using extensive computational resources and tools. (
  • Open standards , data , and software are also key parts of flow cytometry bioinformatics. (
  • In the end you will be able to analyze and visualize your flow data and learn how to get the statistics out of it. (
  • The reader will review May Grunwald Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. (
  • Modular Flow Cytometry Training Courses - These modular courses are linked and have been designed to build up knowledge of flow cytometry to ensure that the delegate is confident to design, carry out, analyse and present their flow cytometry data. (
  • The following phases of developing and validating a robust and reliable flow cytometry assay will be illustrated in this webinar: The critical steps to any flow cytometry assay - panel design, assay development, descriptions of validation test scripts that should be conducted along with suitable acceptance criteria, proper instrument set-up and appropriate controls, gating strategy and identification of cell subsets, and correct interpretation of flow cytometry data. (
  • Designed to assist members of the research community at UMass and on the other Five College campuses to conduct research using flow cytometry and live animal imaging technologies. (
  • GEN: Flow cytometry continues to evolve from its origins as a research tool. (
  • Flow cytometry is a fundamental tool used broadly in cell biological research. (
  • The URMC Flow Cytometry Core sincerely thanks the UR Developmental Center for AIDS Research (P30AI078498, NIH/NIAID) for their support in the acquisition of the Amnis ImageStreamX. (
  • The biotechnology and pharmaceutical companies segment hold a significant share of the hematology and flow cytometry market due to growth in research and development activity. (
  • John Nolan is a Professor at The Scintillon Institute, where his research group develops and applies new technologies for cytometry. (
  • Flow cytometry has found increasing application in the field of apoptosis research. (
  • While the combination of flow cytometry and single-cell technology is still new, it is already defining new developmental states, identifying unculturable microbes, and revealing how cellular heterogeneity relates to health and disease. (
  • The cellular content of FE was measured quantitatively by flow cytometry. (
  • Flow cytometry analyzes fluid by suspending fluid in the light beam or laser beam. (
  • does anybody have a recipe for flow cytometry sheath fluid? (
  • Chargeback costs are quite reasonable considering her expertise on the machine and I would highly recommend using this core to anyone needing flow cytometry. (
  • The Flow Core has designated 3 training levels dependant on a potential users experience with Flow Cytometry and BD based cytometry systems/software. (
  • The mission of URMC Flow Cytometry Core is to provide investigators with state-of-the-art instrumentation along with the human expertise to support all that is possible now, while pushing the limits of what can be done with flow cytometry. (
  • The URMC Flow Core is grateful to NCRR for the funding of a new Aria II high speed cell sorter in part from grant number 1S10RR0292299-01. (
  • Comments about the Flow Core? (
  • The core will have a configuration up that user will be able to access to better enable them to design multi-parametric panels that will work with the instruments found in the NSU Flow Core. (
  • Abcam's Flow Cytometry Lysing Solution is a ready-to-use solution formulated for lysing erythrocytes following monoclonal antibody staining of whole blood. (
  • The FCCIC provides assisted use services (hourly fee) for analytical flow cytometry on all instruments, including the BD LSR Fortessa, BD FACSAria™ Fusion SORP and BD FACSAria™ Fusion. (
  • The flow and genomic cytometry team are available to guide and support users of the services. (
  • Users still need to use the old booking system to access flow cytometry services. (
  • For such a familiar technique, flow cytometry holds many surprises. (
  • there was a description of this technique back in the mid-80s in Cytometry, I think. (
  • Tier II - This level of training is for individuals who have flow cytometry and DIVA experience but not on the BD Fortessa X-20 platform. (
  • He is on the Editorial Boards of Cytometry and Current Protocols in Cytometry, a Fellow of the American Institute of Medical and Biological Engineering (AIMBE), and Past-president of the International Society for Advancement of Cytometry (ISAC). (
  • He is a past President of the International Society for Advancement of Cytometry, is the Editor- in-Chief of Current Protocols in Cytometry, Associate Editor of Histochemica et Cytobiologica, and Associate Editor of Cytometry Part A. He is an active researcher with over 144 peer reviewed publications, 29 book chapters, has edited 9 books and has given over 120 international lectures and taught advanced courses in over a dozen countries. (
  • In its present configuration, the sorter is capable of performing 20 parameters (forward and side scatter plus eighteen color flow cytometry). (
  • A new system for automated flow cytometry sample preparation, the Laminar Wash™ AUTO 1000, offers scientists a fully-automated flow cytometry staining platform designed to produce the most quantitative and reproducible results for flow cytometry. (
  • The size and shape of the antibody used and its conjugates influence the staining measurements in flow cytometry, especially in the case of cytoplasmic or intranuclear staining. (
  • Antibody stability is another concern that affects staining in flow cytometry. (
  • All these factors need to be carefully considered before the formulation of antibody-conjugates for flow cytometry. (
  • Small vesicles have become important because of their biological significance, but instead of trying a number of other techniques, we can now turn to flow to simplify this area. (
  • The new Cytek Northern Lights flow cytometry system makes going beyond 24 colors easy and affordable - offering high sensitivity and greater reagent flexibility that enables scientists to obtain deeper biological insights from a single sample. (
  • A technician preparing a specimen for platelet flow cytometry can add specific tags. (
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