Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Image Cytometry: A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.Regional Blood Flow: The flow of BLOOD through or around an organ or region of the body.Blood Flow Velocity: A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.Laser Scanning Cytometry: A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.Immunophenotyping: Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Antigens, CD: Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Cell SeparationFluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Cell Line, Tumor: A cell line derived from cultured tumor cells.Ploidies: The degree of replication of the chromosome set in the karyotype.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Pulsatile Flow: Rhythmic, intermittent propagation of a fluid through a BLOOD VESSEL or piping system, in contrast to constant, smooth propagation, which produces laminar flow.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Gene Flow: The change in gene frequency in a population due to migration of gametes or individuals (ANIMAL MIGRATION) across population barriers. In contrast, in GENETIC DRIFT the cause of gene frequency changes are not a result of population or gamete movement.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Microspheres: Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.Propidium: Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.Monocytes: Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Fluorescein-5-isothiocyanate: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.Time Factors: Elements of limited time intervals, contributing to particular results or situations.CD4-Positive T-Lymphocytes: A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.Antigens, CD45: High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.Fluoresceins: A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.Leukocytes, Mononuclear: Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Lymphocyte Count: The number of LYMPHOCYTES per unit volume of BLOOD.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Fluorescence: The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Cell-Derived Microparticles: Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Annexin A5: A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.Leukocytes: White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).Lymphocytes: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.Lymphocyte Subsets: A classification of lymphocytes based on structurally or functionally different populations of cells.DNA, Neoplasm: DNA present in neoplastic tissue.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Microscopy, Confocal: A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.B-Lymphocytes: Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.Platelet Activation: A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Rheology: The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.T-Lymphocyte Subsets: A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.CD8-Positive T-Lymphocytes: A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Interferon-gamma: The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Mice, Inbred BALB CBiological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Antigens, CD3: Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).Cerebrovascular Circulation: The circulation of blood through the BLOOD VESSELS of the BRAIN.Blood Platelets: Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.Membrane Glycoproteins: Glycoproteins found on the membrane or surface of cells.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Green Fluorescent Proteins: Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Aneuploidy: The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).Mice, Inbred C57BLBlood Cells: The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.Coronary Circulation: The circulation of blood through the CORONARY VESSELS of the HEART.Antigens, Surface: Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.Antineoplastic Agents: Substances that inhibit or prevent the proliferation of NEOPLASMS.Antigens, CD34: Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.Cell Adhesion: Adherence of cells to surfaces or to other cells.Up-Regulation: A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Dendritic Cells: Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).Flow Injection Analysis: The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.Antigens, CD14: Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.Caspase 3: A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.Phycoerythrin: The metal-free red phycobilin pigment in a conjugated chromoprotein of red algae. It functions as a light-absorbing substance together with chlorophylls.P-Selectin: Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Neoplasm, Residual: Remnant of a tumor or cancer after primary, potentially curative therapy. (Dr. Daniel Masys, written communication)Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.HLA-DR Antigens: A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.Killer Cells, Natural: Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.Receptors, IgG: Specific molecular sites on the surface of various cells, including B-lymphocytes and macrophages, that combine with IMMUNOGLOBULIN Gs. Three subclasses exist: Fc gamma RI (the CD64 antigen, a low affinity receptor), Fc gamma RII (the CD32 antigen, a high affinity receptor), and Fc gamma RIII (the CD16 antigen, a low affinity receptor).Optic Flow: The continuous visual field seen by a subject through space and time.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Cytophotometry: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.T-Lymphocytes, Regulatory: CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Erythrocytes: Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Spleen: An encapsulated lymphatic organ through which venous blood filters.Rhodamine 123: A fluorescent probe with low toxicity which is a potent substrate for P-glycoprotein and the bacterial multidrug efflux transporter. It is used to assess mitochondrial bioenergetics in living cells and to measure the efflux activity of P-glycoprotein in both normal and malignant cells. (Leukemia 1997;11(7):1124-30)Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.HL-60 Cells: A promyelocytic cell line derived from a patient with ACUTE PROMYELOCYTIC LEUKEMIA. HL-60 cells lack specific markers for LYMPHOID CELLS but express surface receptors for FC FRAGMENTS and COMPLEMENT SYSTEM PROTEINS. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli. (From Hay et al., American Type Culture Collection, 7th ed, pp127-8)Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Leukemia, Lymphocytic, Chronic, B-Cell: A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.Antigens, CD19: Differentiation antigens expressed on B-lymphocytes and B-cell precursors. They are involved in regulation of B-cell proliferation.Cell Adhesion Molecules: Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.Antigens, CD56: The 140 kDa isoform of NCAM (neural cell adhesion molecule) containing a transmembrane domain and short cytoplasmic tail. It is expressed by all lymphocytes mediating non-MHC restricted cytotoxicity and is present on some neural tissues and tumors.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Proto-Oncogene Proteins c-bcl-2: Membrane proteins encoded by the BCL-2 GENES and serving as potent inhibitors of cell death by APOPTOSIS. The proteins are found on mitochondrial, microsomal, and NUCLEAR MEMBRANE sites within many cell types. Overexpression of bcl-2 proteins, due to a translocation of the gene, is associated with follicular lymphoma.Laser-Doppler Flowmetry: A method of non-invasive, continuous measurement of MICROCIRCULATION. The technique is based on the values of the DOPPLER EFFECT of low-power laser light scattered randomly by static structures and moving tissue particulates.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.DNA Fragmentation: Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Leukocyte Count: The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.Microfluidic Analytical Techniques: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Antigens, CD4: 55-kDa antigens found on HELPER-INDUCER T-LYMPHOCYTES and on a variety of other immune cell types. CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in MAJOR HISTOCOMPATIBILITY COMPLEX class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as INTERLEUKIN-15 receptors and bind to the HIV receptors, binding directly to the HIV ENVELOPE PROTEIN GP120.Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.Immunomagnetic Separation: A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field. Among other applications, the technique is commonly used to remove tumor cells from the marrow (BONE MARROW PURGING) of patients who are to undergo autologous bone marrow transplantation.Interleukin-2 Receptor alpha Subunit: A low affinity interleukin-2 receptor subunit that combines with the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN to form a high affinity receptor for INTERLEUKIN-2.Antigens, Differentiation, T-Lymphocyte: Antigens expressed on the cell membrane of T-lymphocytes during differentiation, activation, and normal and neoplastic transformation. Their phenotypic characterization is important in differential diagnosis and studies of thymic ontogeny and T-cell function.CD4-CD8 Ratio: Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.K562 Cells: An ERYTHROLEUKEMIA cell line derived from a CHRONIC MYELOID LEUKEMIA patient in BLAST CRISIS.Blood Circulation: The movement of the BLOOD as it is pumped through the CARDIOVASCULAR SYSTEM.Peak Expiratory Flow Rate: Measurement of the maximum rate of airflow attained during a FORCED VITAL CAPACITY determination. Common abbreviations are PEFR and PFR.S Phase: Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.Single-Cell Analysis: Assaying the products of or monitoring various biochemical processes and reactions in an individual cell.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).Antigens, Differentiation: Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Spermatozoa: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.Antigens, CD38: A bifunctional enzyme that catalyzes the synthesis and HYDROLYSIS of CYCLIC ADP-RIBOSE (cADPR) from NAD+ to ADP-RIBOSE. It is a cell surface molecule which is predominantly expressed on LYMPHOID CELLS and MYELOID CELLS.Microfluidics: The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.Coloring Agents: Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.Antigens, CD11b: A CD antigen that contains a conserved I domain which is involved in ligand binding. When combined with CD18 the two subunits form MACROPHAGE-1 ANTIGEN.Carbocyanines: Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Diploidy: The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.Antigens, Neoplasm: Proteins, glycoprotein, or lipoprotein moieties on surfaces of tumor cells that are usually identified by monoclonal antibodies. Many of these are of either embryonic or viral origin.Real-Time Polymerase Chain Reaction: Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Lectins, C-Type: A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Lasers: An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.Organic Chemicals: A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form.Hemorheology: The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.Reactive Oxygen Species: Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of signal transduction and gene expression, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS.Antigens, CD95: A tumor necrosis factor receptor subtype found in a variety of tissues and on activated LYMPHOCYTES. It has specificity for FAS LIGAND and plays a role in regulation of peripheral immune responses and APOPTOSIS. Multiple isoforms of the protein exist due to multiple ALTERNATIVE SPLICING. The activated receptor signals via a conserved death domain that associates with specific TNF RECEPTOR-ASSOCIATED FACTORS in the CYTOPLASM.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cytological Techniques: Methods used to study CELLS.Mice, Nude: Mutant mice homozygous for the recessive gene "nude" which fail to develop a thymus. They are useful in tumor studies and studies on immune responses.Membrane Potential, Mitochondrial: The voltage difference, normally maintained at approximately -180mV, across the INNER MITOCHONDRIAL MEMBRANE, by a net movement of positive charge across the membrane. It is a major component of the PROTON MOTIVE FORCE in MITOCHONDRIA used to drive the synthesis of ATP.Caspases: A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.Lymph Nodes: They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Rhodamines: A family of 3,6-di(substituted-amino)-9-benzoate derivatives of xanthene that are used as dyes and as indicators for various metals; also used as fluorescent tracers in histochemistry.Kinetics: The rate dynamics in chemical or physical systems.Reference Values: The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.Sialic Acid Binding Ig-like Lectin 3: A 67-kDa sialic acid binding lectin that is specific for MYELOID CELLS and MONOCYTE-MACROPHAGE PRECURSOR CELLS. This protein is the smallest siglec subtype and contains a single immunoglobulin C2-set domain. It may play a role in intracellular signaling via its interaction with SHP-1 PROTEIN-TYROSINE PHOSPHATASE and SHP-2 PROTEIN-TYROSINE PHOSPHATASE.Blood Pressure: PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS.Macrophage-1 Antigen: An adhesion-promoting leukocyte surface membrane heterodimer. The alpha subunit consists of the CD11b ANTIGEN and the beta subunit the CD18 ANTIGEN. The antigen, which is an integrin, functions both as a receptor for complement 3 and in cell-cell and cell-substrate adhesive interactions.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Jurkat Cells: A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Photoacoustic Techniques: Investigative and diagnostic methods and procedures based on the photoacoustic effect, which is the generation of SOUND WAVES from the absorption of ELECTROMAGNETIC RADIATION.Fixatives: Agents employed in the preparation of histologic or pathologic specimens for the purpose of maintaining the existing form and structure of all of the constituent elements. Great numbers of different agents are used; some are also decalcifying and hardening agents. They must quickly kill and coagulate living tissue.Cell Size: The quantity of volume or surface area of CELLS.Antigens, Differentiation, Myelomonocytic: Surface antigens expressed on myeloid cells of the granulocyte-monocyte-histiocyte series during differentiation. Analysis of their reactivity in normal and malignant myelomonocytic cells is useful in identifying and classifying human leukemias and lymphomas.Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Antigens, CD8: Differentiation antigens found on thymocytes and on cytotoxic and suppressor T-lymphocytes. CD8 antigens are members of the immunoglobulin supergene family and are associative recognition elements in MHC (Major Histocompatibility Complex) Class I-restricted interactions.Polyploidy: The chromosomal constitution of a cell containing multiples of the normal number of CHROMOSOMES; includes triploidy (symbol: 3N), tetraploidy (symbol: 4N), etc.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Vascular Resistance: The force that opposes the flow of BLOOD through a vascular bed. It is equal to the difference in BLOOD PRESSURE across the vascular bed divided by the CARDIAC OUTPUT.Microcirculation: The circulation of the BLOOD through the MICROVASCULAR NETWORK.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Antigens, CD44: Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)Intercellular Adhesion Molecule-1: A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.Granulocytes: Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.Lipopolysaccharides: Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)Succinimides: A subclass of IMIDES with the general structure of pyrrolidinedione. They are prepared by the distillation of ammonium succinate. They are sweet-tasting compounds that are used as chemical intermediates and plant growth stimulants.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Forkhead Transcription Factors: A subclass of winged helix DNA-binding proteins that share homology with their founding member fork head protein, Drosophila.bcl-2-Associated X Protein: A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein.Interleukin-2: A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Fluorescein: A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium such as the aqueous humor.Drug Synergism: The action of a drug in promoting or enhancing the effectiveness of another drug.Mice, SCID: Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.

Interleukin-8 receptor modulates IgE production and B-cell expansion and trafficking in allergen-induced pulmonary inflammation. (1/36125)

We examined the role of the interleukin-8 (IL-8) receptor in a murine model of allergen-induced pulmonary inflammation using mice with a targeted deletion of the murine IL-8 receptor homologue (IL-8r-/-). Wild-type (Wt) and IL-8r-/- mice were systemically immunized to ovalbumin (OVA) and were exposed with either single or multiple challenge of aerosolized phosphate-buffered saline (OVA/PBS) or OVA (OVA/OVA). Analysis of cells recovered from bronchoalveolar lavage (BAL) revealed a diminished recruitment of neutrophils to the airway lumen after single challenge in IL-8r-/- mice compared with Wt mice, whereas multiply challenged IL-8r-/- mice had increased B cells and fewer neutrophils compared with Wt mice. Both Wt and IL-8r-/- OVA/OVA mice recruited similar numbers of eosinophils to the BAL fluid and exhibited comparable degrees of pulmonary inflammation histologically. Both total and OVA-specific IgE levels were greater in multiply challenged IL-8r-/- OVA/OVA mice than in Wt mice. Both the IL-8r-/- OVA/OVA and OVA/PBS mice were significantly less responsive to methacholine than their respective Wt groups, but both Wt and IL-8r mice showed similar degrees of enhancement after multiple allergen challenge. The data demonstrate that the IL-8r modulates IgE production, airway responsiveness, and the composition of the cells (B cells and neutrophils) recruited to the airway lumen in response to antigen.  (+info)

Cystic fibrosis transmembrane conductance regulator-mediated corneal epithelial cell ingestion of Pseudomonas aeruginosa is a key component in the pathogenesis of experimental murine keratitis. (2/36125)

Previous findings indicate that the cystic fibrosis transmembrane conductance regulator (CFTR) is a ligand for Pseudomonas aeruginosa ingestion into respiratory epithelial cells. In experimental murine keratitis, P. aeruginosa enters corneal epithelial cells. We determined the importance of CFTR-mediated uptake of P. aeruginosa by corneal cells in experimental eye infections. Entry of noncytotoxic (exoU) P. aeruginosa into human and rabbit corneal cell cultures was inhibited with monoclonal antibodies and peptides specific to CFTR amino acids 108 to 117. Immunofluorescence microscopy and flow cytometry demonstrated CFTR in the intact murine corneal epithelium, and electron microscopy showed that CFTR binds to P. aeruginosa following corneal cell ingestion. In experimental murine eye infections, multiple additions of 5 nM CFTR peptide 103-117 to inocula of either cytotoxic (exoU+) or noncytotoxic P. aeruginosa resulted in large reductions in bacteria in the eye and markedly lessened eye pathology. Compared with wild-type C57BL/6 mice, heterozygous DeltaF508 Cftr mice infected with P. aeruginosa had an approximately 10-fold reduction in bacterial levels in the eye and consequent reductions in eye pathology. Homozygous DeltaF508 Cftr mice were nearly completely resistant to P. aeruginosa corneal infection. CFTR-mediated internalization of P. aeruginosa by buried corneal epithelial cells is critical to the pathogenesis of experimental eye infection, while in the lung, P. aeruginosa uptake by surface epithelial cells enhances P. aeruginosa clearance from this tissue.  (+info)

Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. (3/36125)

Hermansky-Pudlak syndrome (HPS) is a genetic disorder characterized by defective lysosome-related organelles. Here, we report the identification of two HPS patients with mutations in the beta 3A subunit of the heterotetrameric AP-3 complex. The patients' fibroblasts exhibit drastically reduced levels of AP-3 due to enhanced degradation of mutant beta 3A. The AP-3 deficiency results in increased surface expression of the lysosomal membrane proteins CD63, lamp-1, and lamp-2, but not of nonlysosomal proteins. These differential effects are consistent with the preferential interaction of the AP-3 mu 3A subunit with tyrosine-based signals involved in lysosomal targeting. Our results suggest that AP-3 functions in protein sorting to lysosomes and provide an example of a human disease in which altered trafficking of integral membrane proteins is due to mutations in a component of the sorting machinery.  (+info)

Enhanced myocardial glucose use in patients with a deficiency in long-chain fatty acid transport (CD36 deficiency). (4/36125)

CD36 is a multifunctional, 88 kDa glycoprotein that is expressed on platelets and monocytes/macrophages. CD36 also has high homology with the long-chain fatty acid (LFA) transporter in the myocardium. Although platelet and monocyte CD36 levels can indicate a CD36 deficiency, they cannot predict specific clinical manifestations in the myocardium of a given person. We examined the hypothesis that a deficiency in LFA transport augments myocardial glucose uptake in patients with a type I CD36 deficiency. METHODS: Seven fasting patients with a type I CD36 deficiency and 9 controls were assessed by cardiac radionuclide imaging using beta-methyl-p-iodophenyl-pentadecanoic acid (BMIPP) as a LFA tracer and by PET with 18F-fluorodeoxyglucose (FDG). RESULTS: None of the patients with a CD36 deficiency showed myocardial uptake of BMIPP. The percentage dose uptake of BMIPP in these subjects was significantly lower than that in normal controls (1.31+/-0.24 versus 2.90+/-0.2; P < 0.005). PET studies revealed that myocardial FDG accumulation was substantially increased in patients with a CD36 deficiency. Quantitative analysis showed that the percentage dose uptake of FDG in patients with a CD36 deficiency was significantly higher than that in normal controls (1.28+/-0.35 versus 0.43+/-0.22; P< 0.01). CONCLUSION: CD36 functions as a major myocardial LFA transporter and its absence may cause a compensatory upregulation of myocardial glucose uptake.  (+info)

Proliferation and differentiation of rat theca-interstitial cells: comparison of effects induced by platelet-derived growth factor and insulin-like growth factor-I. (5/36125)

This study was designed to evaluate mechanisms regulating proliferation of steroidogenically active and steroidogenically inactive theca-interstitial (T-I) cells, and, specifically, to evaluate the effects of platelet-derived growth factor (PDGF) and insulin-like growth factor-I (IGF-I). T-I cells obtained from immature Sprague-Dawley rats were cultured in chemically defined media. Proliferation was assayed by thymidine incorporation and cell counting. Steroidogenically active cells were identified by the presence of 3beta-hydroxysteroid dehydrogenase activity. Flow cytometry facilitated separation of dividing cells (in S and G2/M phases of the cell cycle) from nondividing cells (in G0 and G1 phases of the cell cycle). PDGF alone (0.1-1 nM) produced a dose-dependent increase in DNA synthesis by up to 136%. IGF-I alone (10 nM) increased DNA synthesis by 56%. In the presence of both IGF-I (10 nM) and PDGF (0.1-1 nM), DNA synthesis increased by 108-214%. PDGF (1 nM) increased the total number of T-I cells by 43%; this effect was due to an increase in the number of steroidogenically inactive cells (47%). In contrast, the stimulatory effect of IGF-I (10 nM) was predominantly due to an increase in the number of steroidogenically active cells (163%). Separation of dividing cells from nondividing cells was accomplished with the aid of flow cytometry. In the absence of growth factors, the proportion of steroidogenically active cells was 35% lower among proliferating than resting cells. PDGF (1 nM) decreased the proportion of steroidogenically active cells among both proliferating and resting cells (by 43% and 16%, respectively). In contrast, IGF-I (10 nM) increased the proportion of steroidogenically active cells among proliferating cells by 56%. These findings indicate that differentiated/steroidogenically active cells divide; furthermore, PDGF and IGF-I may selectively stimulate proliferation of individual subpopulations of T-I cells, thereby providing a mechanism for development of structural and steroidogenically active components of the T-I compartment.  (+info)

Use of RhD fusion protein expressed on K562 cell surface in the study of molecular basis for D antigenic epitopes. (6/36125)

The human D antigens, one of the most clinically important blood groups, are presented by RhD protein with a putative 12 transmembrane topology. To understand the molecular basis for the complex antigenic profile of RhD protein, we expressed a series of RhD fusion proteins using different portions of Duffy protein as a tag in erythroleukemic K562 cells. Because the reactivity of monoclonal anti-RhD antibody, LOR15C9, depends mainly on the sequence coded by exon 7 of RhD, we altered DNA sequence corresponding to the amino acid residues 323-331(A) and 350-354(B) in the exon 7. The mutation in region B resulted in a severe reduction in LOR15C9 binding by flow cytometry analysis, suggesting that region B may play an important role in constituting antigen epitopes recognized by LOR15C9. On the other hand, a slight decrease in the antibody binding was observed for the region A mutant, suggesting that the intracellularly located region A may elicit a long distance effect on the formation of exofacial antigen epitopes. In addition, using various monoclonal antibodies against RhD, we compared the antigenic profile of expressed RhD fusion protein with that of endogenous RhD in K562 cells as well as in erythrocytes.  (+info)

Phenotypic and functional characterisation of myofibroblasts, macrophages, and lymphocytes migrating out of the human gastric lamina propria following the loss of epithelial cells. (7/36125)

BACKGROUND: The basement membrane of human colonic mucosa contains numerous discrete pores. We have recently shown that following loss of the surface epithelium, many cells migrate out of the colonic lamina propria via basement membrane pores. AIMS: To characterise cells migrating out via basement membrane pores of the human gastric lamina propria, following loss of the surface epithelium. METHODS: Fresh human gastric mucosal samples were completely denuded of epithelial cells and placed in culture. Tissue samples were studied by electron microscopy (EM) and cells by EM, FACS analysis, immunohistochemistry, and reverse transcription polymerase chain reaction (RT-PCR). RESULTS: EM showed numerous discrete pores (0. 65-8.29 microm in diameter) in the subepithelial basement membrane. During culture of mucosal samples denuded of epithelial cells, lymphocytes, macrophages, and myofibroblasts migrated out of the lamina propria via the basement membrane pores. The lymphocytes were predominantly CD45RO+ and CD69+ T cells. Macrophages were shown to express cyclooxygenase (COX) 1 and 2 enzymes. Myofibroblasts were established in culture and, despite prolonged culture and passage, retained their phenotype. They expressed mRNA and protein for COX 1 and 2 enzymes and their release of prostaglandin E2 was inhibited by selective COX 1 and 2 inhibitors. CONCLUSIONS: Lamina propria cells migrating out of cultured denuded gastric mucosal samples have been characterised phenotypically and functionally. Such cells would be suitable for studies of their interactions with epithelial cells and also with Helicobacter pylori and its products.  (+info)

Adenovirus mediated p53 tumour suppressor gene therapy for human gastric cancer cells in vitro and in vivo. (8/36125)

BACKGROUND/AIMS: Gastric cancer is one of the most prevalent forms of cancer in East Asia. Point mutation of the p53 gene has been reported in more than 60% of cases of gastric cancer and can lead to genetic instability and uncontrolled cell proliferation. The purpose of this investigation was to evaluate the potential of p53 gene therapy for gastric cancer. METHODS: The responses of human gastric cancer cell lines, MKN1, MKN7, MKN28, MKN45, and TMK-1, to recombinant adenoviruses encoding wild type p53 (AdCAp53) were analysed in vitro. The efficacy of the AdCAp53 treatment for MKN1 and MKN45 subcutaneous tumours in nude mice was assessed in vivo. RESULTS: p53-specific growth inhibition was observed in vitro in two of four gastric cancer cell lines with mutated p53, but not in the wild type p53 cell line. The mechanism of the killing of gastric cancer cells by AdCAp53 was found, by flow cytometric analysis and detection of DNA fragmentation, to be apoptosis. In vivo studies showed that the growth of subcutaneous tumours of p53 mutant MKN1 cells was significantly inhibited by direct injection of AdCAp53, but no significant growth inhibition was detected in the growth of p53 wild type MKN45 tumours. CONCLUSIONS: Adenovirus mediated reintroduction of wild type p53 is a potential clinical utility in gene therapy for gastric cancers.  (+info)

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Traditional flow cytometry methods have been remarkably successful at detecting and sorting specific cells in a sample consisting of many cell types. However, a significant limitation of these methods is their inability to be applied in-vivo. Our research focuses on creating and perfecting novel flow cytometry methods which resolve this problem. Previous work includes the development of a two-photon in-vivo flow cytometry system. The use of multiphoton excitation creates an extremely narrow excitation region, making it possible to record signals from single cells as they propagate through the bloodstream. This eliminates the complicated fluid dynamics required for conventional flow cytometry, and also allows the user to focus on a single blood vessel in a living animal. Current and future work includes investigations of coherent control and the use of fiber optics for monitoring in the bloodstream itself.. ...
We report the development of a novel flow cytometry-based Ig capture assay (ICA) for the identification and sorting of individual Ab-secreting cells based on their Ag reactivity. The ICA represents a fast and versatile tool for single-cell sorting of peripheral plasmablasts, streamlining subsequent Ab analysis, and cloning. We demonstrate the utility of the assay by isolating Ag-reactive plasmablasts from cryopreserved PBMC obtained from volunteers vaccinated with a recombinant HIV envelope protein. To show the specificity of the ICA, we produced Ag-specific Abs from these cells and subsequently verified their Ag reactivity via ELISA. Furthermore, we used the ICA to track Ag-specific plasmablast responses in HIV-vaccine recipients over a period of 42 d and performed a head-to-head comparison with a conventional B cell ELISpot. Results were highly comparable, highlighting that this assay is a viable alternative for monitoring Ag-specific plasmablast responses at early time points after infection ...
Commercial organizations. Regional Segments:. Regional segmentation details the regional aspects of the global Flow Cytometry market. and explains the restrictive framework thats probably to impact the market. It highlights the political scenario in the market and the anticipates its influence on the global Flow Cytometry market.. • Middle East and Africa (Egypt and GCC Countries). • North America (United States, Mexico, and Canada). • South America (Brazil etc.). • Europe (Germany, Turkey, Russia UK, Italy, France, etc.). Get UPTO 25% OFF On Flow Cytometry Market Report (Valid Till 15Jan 2020). Inquire/Speak To Expert for Further Detailed Information About Flow Cytometry Report: https://marketresearch.biz/report/flow-cytometry-market/#inquiry. Table of Contents:. Chapter One: Global Flow Cytometry Market Overview. 1.1 Flow Cytometry Preface. Chapter Two: Global Flow Cytometry Market Analysis. 2.1 Flow Cytometry Report Description. 2.1.1 Flow Cytometry Market Definition and Scope. 2.2 ...
Flow cytometry is conventionally used to measure cell-surface antigen expression. However, many antigens are found within the cytoplasm, and it is necessary to fix and permeabilize cells to enable antibodies to gain access to them. In this study we have established the conditions for studying intracellular antigens in human trophoblast cells by flow cytometry using an antibody to TAP1 (a key molecule in the process of Class I MHC assembly). We have previously shown by immunocytochemistry that TAP1 expression is apparently greater on Class 1 positive extravillous cytotrophoblast than on any other fetal or maternal tissue. However, as immunohistochemistry is not quantitative we have used three-colour flow cytometry to measure the expression of TAP1 in different trophoblast populations. Villous and extravillous cytotrophoblast were identified in first trimester and term placental and decidual digests on the basis of their expression of cytokeratin and Class I MHC antigens. The level of expression of TAP1
Hi Philipp, You can get devel versions of R at ftp://ftp.stat.math.ethz.ch/Software/R/. I believe the idea has always been that BioC devel (i.e., 2.6) works with R devel; iFlow is in BioC 2.6 so that should work. However, iFlow may not necessarily need the latest R. I havent tried the latest version but I have been playing with iFlow back in 2009 using R 2.9 or so. You can install iFlow from sources from the SVN repository (https://hedgehog.fhcrc.org/bioconductor/trunk/madman/Rpacks) user readonly, password readonly. To install it, run the R CMD INSTALL ,path to your iFlow,. The only pain with this approach is that you will have to manually resolve all the dependencies. Good luck. Cheers, Josef , Date: Mon, 08 Feb 2010 11:52:41 +0100 , From: Philipp Meng ,philipp.meng at meduniwien.ac.at, , To: Martin Morgan ,mtmorgan at fhcrc.org, , Cc: bioconductor ,bioconductor at stat.math.ethz.ch, , Subject: Re: [BioC] Installing problems of Flow Cytometry on Ubuntu , Karmic , Message-ID: , ...
Some snippets from your converted document: Designing and Implementing a High-Level Multicolor Flow Cytometry Assay Brent Wood MD PhD Department of Laboratory Medicine University of Washington Define Purpose of Assay • Most important question - What information is required? - What information is most important? • Prioritize • Compromises are inevitable - Simplest assay is best Outline • Instrument optimization • Compensation • Reagent selection and optimization • Panel validation • Data analysis Detector Optimization Detector Optimization PMT Voltage Green = 400 V, Blue = 450 V, Red = 500 V, Violet = 550 V Detector Voltage 475 volts 575 volts 675 volts 775 volts 875 volts • Too low reduces sensitivity • Too high pushes bright signals off scale - Dilute with unlabeled antibody • May need to compromise Optimization • Daily optimization not practical • Once optimized, do NOT change instrument settings unless change in performance - New optical filters - New PMT ...
Background: The neutral comet assay was devised to measure double-stranded DNA breaks, but it has also been used to measure apoptosis based on its characteristic DNA fragmentation patterns. There is still uncertainty about the reliability of this method. By comparing the comet assay with a flow cytometry method that uses Annexin V binding to apoptotic cells, we have provided further evidence for evaluating the usefulness of the comet assay for detecting apoptosis. Methods: Apoptosis was induced in human peripheral blood mononuclear cells (PBMC) by ionizing radiation and measured using the comet assay and a flow cytometry method that measures Annexin V and propidium iodide (PI) staining. Results: The Annexin V flow cytometry assay distinguished among early apoptosis, late apoptosis, and an apoptotic or necrotic phase in which the cells were labeled with both Annexin V and PI. The comet assay detected only the latter two phases of apoptosis. Conclusions: The comet assay is a useful tool for ...
TY - JOUR. T1 - Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples. AU - D'Alessio, F.. AU - Mirabelli, P.. AU - Gorrese, M.. AU - Scalia, G.. AU - Gemei, M.. AU - Mariotti, E.. AU - Di Noto, R.. AU - Martinelli, P.. AU - Fortunato, G.. AU - Paladini, D.. AU - Del Vecchio, L.. PY - 2011/1. Y1 - 2011/1. N2 - During the last decades, extended characterizations were performed of human full-term cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. First, we observed that the percentage of CD34 PosCD45 Dim cells was higher in hEPCB compared with hTCB and that it decreased during 16th-20th week of pregnancy. Within the CD34 PosCD45 Dim population, we examined the expression of CD29, CD31, CD38, CD90, CD117, CD133, CD135, CD200, CD243, and CD338. We found that CD135 ...
The CellROX Orange Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX Orange Reagent, as well as SYTOX Red Dead Cell Stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-b
A fundamental tenet of scientific research is that the published results of any study have to be open to independent validation or refutation. The Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) establishes criteria for recording and reporting information about the flow cytometry experiment overview, samples, instrumentation and data analysis. It promotes consistent annotation of clinical, biological and technical issues surrounding a flow cytometry experiment by specifying the requirements for data content and by providing a structured framework for capturing information ...
Author(s): Uitrakul S, Hutton C, Veal GJ, Jamieson D. Publication type: Article. Publication status: Published. Journal: European Journal of Clinical Investigation. Year: 2019. Volume: 49. Issue: 7. Print publication date: 27/06/2019. Online publication date: 31/03/2019. Acceptance date: 26/03/2019. ISSN (print): 0014-2972. ISSN (electronic): 1365-2362. Publisher: Wiley-Blackwell. URL: https://doi.org/10.1111/eci.13115. DOI: 10.1111/eci.13115. ...
The aim of this study was to compare DNA content in hepatocyte and erythrocyte nuclei of the European sunbleak, Leucaspius delineatus, in relation to nuclear and cell size by means of flow cytometry and fluorescence microscopy. The DNA standards, chicken and rainbow trout erythrocytes, were prepared in parallel with both cell types, with initial separation of liver cells in pepsin solution followed by cell filtering. Standards and investigated cells were stained with a mixture of propidium iodide, citric acid, and Nonidet P40 in the presence of RNAse, and fluorescence of at least 50,000 nuclei was analyzed by flow cytometry. Average cell size was determined by flow cytometry, using fresh cell suspension in relation to latex beads of known diameter. The size of nuclei was examined on the basis of digital micrographs obtained by fluorescence microscopy after nuclei staining with DAPI. The sunbleaks erythrocyte nuclei contain 2.25 ± 0.06 pg of DNA, whereas the hepatocyte nuclei contain 2.46 ± ...
Investigation of signal transduction mechanisms is important for development of therapy and understanding complex life systems. In this study, to establish a novel recognition method of signal transduction pathways, we investigated signal transducers using flow cytometry. @The flow cytometric measurement shows a mean phosphorylation level (mean of fluorescence intensity, MFI) and a deviation of the phosphorylation level (coefficient variation, CV) in a cluster of cells. As a model of signal pathways, Jurkat cells (T cell leukemia cell line) were stimulated with interleukin-21 or interferon- , and signal transducers and activators of transcription (STATs) and extracellular signal-regulated kinase (ERK) 1/2 were measured using flow cytometry. Furthermore, peripheral blood was stimulated, and then various signal transducers of the lymphocytes and neutrophils were analyzed with MFI and CV. @After the stimulation, the increase of STATs MFI induced a temporal change of CV. On the other hand, the ...
Sysmex analysers use the fluorescence flow cytometry method in the reticulocyte channel to measure fragmented red blood cells (FRC% and FRC#) as research parameters.. A specific area below the RBC area in the RET scattergram is used for identification of fragmented red blood cells. Due to the absence of nucleic acids in red blood cells the intensity of the measured side fluorescence signals (SFL) is extremely low. In addition, the high-angle forward scatter (FSC) is lower than that of intact red blood cells.. ...
Spring 2017 Fluorescence Calibration study - John Nolan. This years study aims to demonstrate a consensus approach to calibration and reporting of EV fluorescence measurements. The study has three tasks:. 1) Calibrate instrument fluorescence response using commercial reagents,. 2) Measure a common set of EV reference materials using a diverse set of flow cytometry methods and instruments, and report the data in a uniform manner such that results can be compared across methods and instruments, and. 3) Evaluate a prototype set of EV-scale fluorescence intensity and antibody binding standards that may be more appropriate for calibration and standardization of EV measurements.. All participants will perform a common set of measurements and report their methods and results according to draft Minimum Information about an EV Flow Cytometry measurement (MI EV FlowCyt) guidelines. The methods, results and guidelines that result from the study will be posted here and reported in a manuscript to be ...
The phosphatidylinositol 3-kinase (PI3K) pathway plays an important role in multiple myeloma (MM), a blood cancer associated with uncontrolled proliferation of bone marrow plasma cells. This study aimed to develop a robust clinical pharmacodynamic (PD) assay to measure the on-target PD effects of the selective PI3K inhibitor GDC-0941 in MM patients. We conducted an in vitro drug wash-out study to evaluate the feasibility of biochemical approaches in measuring the phosphorylation of S6 ribosomal protein (S6), one of the commonly used PD markers for PI3K pathway inhibition. We then developed a 7-color phospho-specific flow cytometry assay, or phospho flow assay, to measure the phosphorylation state of intracellular S6 in bone marrow aspirate (BMA) and peripheral blood (PB). Integrated mean fluorescence intensity (iMFI) was used to calculate fold changes of phosphorylation. Assay sensitivity was evaluated by comparing phospho flow with Meso Scale Discovery (MSD) and immunohistochemistry (IHC) assays.
Human induced pluripotent cells (iPSCs) were obtained from the HipSci project (http://www.hipsci.org) and differentiated into macrophages using an established protocol (van Wilgenburg, 2013). The genotype_id column of the flow_sample_metadata.txt file contains the canonical HipSci iPSC line name from which the macrophages were differentiated. Data acquisition We used flow cytometry to measure the cell surface expression of three canonical macrophage markers: CD14, CD16 (FCGR3A/FCGR3B) and CD206 (MRC1). Macrophages were cultured in 10 cm tissue-culture treated plates and detached from the plates by incubation in 6 mg/ml lidocaine-PBS solution (Sigma L5647) for 30 minutes followed by gentle scraping. From each cell line we harvested between 300,000-500,000 cells. Detached cells were washed in media, centrifuged at 1200 rpm for 5 minutes and resuspended in flow cytometry buffer (2% BSA, 0.001% EDTA in D-PBS) and split into two wells of a 96-well plate. Nonspecific antibody binding sites were blocked by
Based on long-standing expertise in multicolor flow cytometry, we have compiled a selection of resources that support you in setting up multicolor assays. - Lëtzebuerg
The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the UV laser of the flow cytometer. The Click-iT Plus formulation
Browse Flow cytometry products on Labviva. Find relevant scientific protocols, papers and to help find the right product for your application.
Flowcytometry provides an unequalled technique for the analysis and sorting of specific cell types in the context of larger populations of cells. Combined use of defined flow cytometric reagents not only allows to identify minute numbers of specific cells in a total population but also enables to elucidate key aspects of their biological function. Moreover, flow cytometric cell sorting techniques, up to the level of single cell sorting, permit further investigation and modulation of specific cell populations for scientific or clinical purposes under experimentally defined conditions. Since flowcytometry is a technique employed by many researchers, the UMCG has stationed the available flow cytometry equipment in a core facility: the Flow Cytometry Unit (FCU). For research purposes, the FCU accommodates 5 analytical flow cytometers (two FACSCaliburs, one FACSVerse and one LSR-II from BD Biosciences and a MACSQuant from Miltenyi Biotec). For sorting the facility has two high speed multicolor cell ...
FMO controls are samples that contain all the antibodies you are testing in your experimental samples, minus one of them. When analyzing the minus, or left out parameter in an FMO control, you give yourself a strong negative control to work with. Its a strong negative control because the left out marker in the FMO control allows you to take into account how the other stains in your panel affect the respective minus parameter. Many flow cytometry gates are difficult to define. This is especially true when youre looking at activation markers within a continuum or accounting for the large data spread that occurs when compensating a 10+ color experiment. The only way to convince reviewers that your gate is in the proper place is by using FMO controls. Heres why you need to use FMO controls for any multicolor flow cytometry experiment and how to prepare these controls properly.. Read More ...
We performed serial quantification of the PNH clone size in 38 samples using multiparameter flow cytometry. Granulocytes, monocytes, and RBCs were gated using forward and side scatter as well as lineage-specific markers. The GPI-linked markers fluorescent aerolysin (FLAER), CD55, and CD59 were comparatively evaluated. We also evaluated CD16 on granulocytes and CD14 on monocytes. The sensitivity of detection by each marker was further defined by serial dilution experiments on a flow-sorted sample. Two patients had quantification of their GPI-negative clones before and after allogeneic BMT. ...
Laboratory directors who routinely utilize flow cytometry for at least part of their diagnostic evaluations in leukemias or lymphomas were surveyed by mail. The survey consisted of 12 questions about the flow cytometry procedures used by the laboratory in evaluating leukemias and lymphomas and on the format and content of their official report. It also requested an example of a typical leukemia/lymphoma report and solicited write-in comments about additional important aspects of using flow cytometry to evaluate leukemias and lymphomas not covered by the questionnaire. The goal of the survey, which was sponsored by the Clinical Cytometry Society (CCS), was to document what directors of flow cytometry laboratories currently consider to be the appropriate contents of a clinical leukemia/lymphoma phenotyping analysis and in what manner and detail they report such flow cytometry results to clinicians. The survey indicated that a large number of markers are routinely evaluated to phenotype leukemias ...
Purpose: Chronic lymphocytic leukemia (CLL) is currently incurable with standard chemotherapeutic agents, highlighting the need for novel therapies. Overcoming proliferative and cytoprotective signals generated within the microenvironment of lymphoid organs is essential for limiting CLL progression and ultimately developing a cure.. Experimental Design: We assessed the potency of cyclin-dependent kinase (CDK) inhibitor CR8, a roscovitine analog, to induce apoptosis in primary CLL from distinct prognostic subsets using flow cytometry-based assays. CLL cells were cultured in in vitro prosurvival and proproliferative conditions to mimic microenvironmental signals in the lymphoid organs, to elucidate the mechanism of action of CR8 in quiescent and proliferating CLL cells using flow cytometry, Western blotting, and quantitative real-time PCR.. Results: CR8 was 100-fold more potent at inducing apoptosis in primary CLL cells than roscovitine, both in isolated culture and stromal-coculture conditions. ...
If you find yourself starting to plan a clinical flow cytometry assay, here are the top 3 issues to think about as you plan your experiment.
Video articles in JoVE about protein subunits include Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies, Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes, Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy, The MultiBac Protein Complex Production Platform at the EMBL, High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast, Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells, Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly, Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method, A Flow Cytometry-Based Assay to Identify Compounds That Disrupt Binding of Fluorescently-Labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4, Visualization of ATP Synthase Dimers in
We present a novel automated methodology that compensates for the effect of cell morphology on flow cytometry data, and thereby enables a quantitative analysis of high‐throughput flow cytometry data. The algorithm normalizes the effect of the physical characteristics of cell size and cell granularity on the fluorescence intensity, thereby enabling the analysis of fluorescence intensities (protein abundance) in the presence of different morphological characteristics of cells in a population. In contrast to traditional gating, which discards the large majority of cells, the regression model retains all cells and thereby provides more accurate statistics, higher consistency across replicates and the ability to handle biological samples that contain far fewer cells (at least 10‐fold), allowing for faster and cheaper data acquisition. This is relevant when one is looking for rare cells (e.g., stem cells), or when performing high‐throughput screens where only a few hundred cells per experimental ...
As a leading supplier of research products and custom services to global academic, pharmaceutical and biotech communities, Creative Diagnostics recently launches flow cytometry for all bio-researchers focusing on scientific study. The flow cytometry data is extremely quantitative and it provides fast, objective and simultaneous multi-parameter analysis of single cells as well as physical separation of cells of particular interest. This flow cytometry is for research use only, not for use in diagnostic procedures.. Flow cytometry is a laser-based technique to count and analyze the size, shape and properties of individual cells within a heterogeneous population of cells. Flow cytometry is a widely used approach to the phenotype of the cells and to the assessing of the purity of isolated subpopulations. Predominantly, it is measured by the fluorescence intensity resulted from fluorescent-conjugated antibodies directly recognizing target proteins, or ligands specifically binding to molecules (such ...
Leveraging their expertise from successes in the Collaboration for AIDS Vaccine Discovery (CAVD), the Vaccine Statistical Support platform will assemble interdisciplinary teams to collaborate on specific vaccine research projects in BMGF-priority infectious diseases beyond CAVD. The team will provide state-of-the-art statistical computational methods for study design and data analyses. Specific areas of expertise and novel methodological development are the analysis of immunologic checkerboard data, signal processing, normalization and analysis of flow cytometry-based assays, immunologic correlates of protection, sieve analyses, and repeated low-dose challenge experiments in animal model systems. The team also designs data models for novel immunologic assays and supports data sharing and collaboration.. Through its work on each project, the statistical team identifies best practices for study designs and data analysis and may advise on standard nomenclatures and data formats to facilitate data ...
Alcuni citometri a flusso utilizzano laser a semiconduttori (633 nm) e coloranti fluorescenti per acidi nucleici che separano i leucociti utilizzando i segnali di:. Forward Scatter (FSc) Side Fluorescence (SFl) Side Scatter (SSc);. Leucocytes. Lymphocytes Monocytes/Macrophages Granulocytes:...
Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitement; therefore, it must be possible to mark the particles with one or more fluorescent substances. Information is also collected regarding the size and structural complexity of each particle. This multiparameter study of each particle enables us to analyse subpopulations in complex samples through electronic sorting. Some cytometers are also equipped with sorting and collection modules for particles of interest.. The most important applications of flow cytometry include those relating to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzyme activity, cell integrity and membrane permeability and calcium flows.. The Unit currently hosts five analyzers and two ...
Research Application. Imperative data on development opportunities, market hazards in Flow Cytometry Instrument industry will delineate the business execution at present and in not so distant future. Flow Cytometry Instrument Industry plans and approaches, new product launch events, mergers and securing and innovative headways are clarified. The upstream raw material providers of Flow Cytometry Instrument, manufacturing base, cost structures and production process examination are broke down. Likewise, the marketing channels of Flow Cytometry Instrument industry, downstream purchasers, work cost included and value structures are expounded.. The Global Flow Cytometry Instrument market value and growth rate for each application, type and region is studied from 2013-2018. The import-export details, production and consumption status of Flow Cytometry Instrument Market is provided for every region and key countries present in this region. Furthermore, the SWOT analysis to predict the Flow Cytometry ...
Este artículo ilustra un método eficaz para cuantificar las mitocondrias o lisosomas en las células vivas. La combinación de tintes...
... ,FACSCalibur is the only four-color, automated benchtop flow cytometry system that can perform both analysis and cell sorting. Designed specifically to support a wide range of applications, the FACSCalibur system offers software instrument control, auto-sample loading, and push-button fluidic contro,medicine,medical supply,medical supplies,medical product
Another issue is flow cytometry analyses. As mentioned above, flow cytometry is essential when assessing BM involvement. Current guidelines regard a small clonal population (, 2%) as uninvolved BM [30]. However, to the best of our knowledge, no clinical study has addressed this issue. Moreover, recent advances in flow cytometry technology have caused a wide range of variation in the sensitivity of flow cytometric analyses among institutions. Thus, it is desirable to document the minimal criteria for flow cytometry technology in a standard guideline. The last issue in flow cytometry is the meaning of discordance between a bone marrow biopsy (BMB) and flow cytometry. Recently, in a study that included 757 NHL patients, there was considerable discordance between BMB and flow cytometry [33]. For example, in FL and LPLs, the discordance rates were up to 22% and 24%, respectively. Based on BMB and flow cytometry results, four subsets can be created in patients with NHL: positive BMB and positive flow ...
Background Non specific inflammation contributes to the pathogenesis of Heart Failure (HF) through a complex interaction between endothelial cells, platelets, and leukocytes. Leukocyte expression of CD11b, a well-known marker of leukocyte activation, and levels of cytoplasmic nitric oxide (NO), an important anti-inflammatory mediator, can be readily measured by flow cytometry.. Objectives: The purpose of this investigation was to determine the degree of expression of activation marker CD11b in leukocytes as well as levels of nitric oxide (NO) in leukocytes in patients with different states of HF using flow cytometry methods.. Methods Patients with known HF were divided in three different groups: NHYA I-II, NYHA III-IV (defined as chronic stable New York Heart Association symptomatology) and Acutely Decompensated HF (defined as HF with a relatively rapid onset of signs and symptoms, resulting in hospitalization or unplanned office or emergency room visits). A peripheral blood sample was obtained ...
Following this seminar, the attendee should be able to establish this method in their own laboratories, which will facilitate transfers of assays developed at their sites to other sites with who have also standardized their instruments with this new method.
Flow cytometry to verify murine BMDCs problem - posted in Flow Cytometry: Hi everyone. Hoping someone could shed some light.... Ive been having real issues with staining murine bone marrow DCs positive for CD11c and MHCII. Currently about 1% of the non-adherent cells I collect are staining on flow cytometry as CD11c+ and about 30-40% as MHCII +. I have no idea what Im doing wrong as morphologically they look like dendritic cells! My protocol is as follows: I...
Stroke places a serious health burden on our society in terms of mortality, morbidity, and economic cost. Leukocyte-mediated inflammation is known to damage the brain several hours to days following stroke. However, it is unclear whether leukocytes accumulate and cause injury when blood is first returned to the brain. Therefore, the specific aims of this proposal are to 1) identify the exact locations of leukocyte accumulation in the rat cerebral microcirculation during the first 60 minutes of reperfusion following stroke using the clinically relevant model of stroke, middle cerebral artery occlusion, and in-vivo video microscopy 2) examine the contribution of the cytokine, interleukin-1 (IL-1) to early leukocyte retention by measuring the levels of IL-1 in brain and peripheral blood, by examining the effect of IL-1 on leukocyte adhesion molecule CD1b expression and oxygen radical production with flow cytometric techniques, and by pretreating animals with stroke with an IL-1 receptor antagonist ...
Join Dr. Kevin Shoulars, from Duke University Medical Center, as he discusses his work with Dr. Joanne Kurtzberg on the development of a rapid flow cytometry-based method for measuring the potency of cord blood units.
Hello, I am writing a chapter on flow cytometry in bivalve pathology for a marine biotechnology book. I am having some trouble deciding why my research on differential hemocyte counts in oysters using log90 vs FLS differs from other research on oysters and clams. I get 3 consistent hemocyte subpopulations while other people get 1 broad group and I would like to be able to tell if this is real or if it is the difference between log and linear signal collection. The methods mention that side scatter was used and the axis of the plots is FLS vs SSC. In other studies (mammalian), I see 3 or so wbc groups in histograms labelled FLS vs SSC and they talk about side scatter in the text. Does SSC or side scatter ever imply that log scale was used or should I assume that SSC is a linear scale? There are no numbers along the axes that would help me. Thank you, Kathy Alcox ************************************* Kathryn A. Ashton-Alcox Haskin Shellfish Research Laboratory Rutgers University 6959 Miller ...
Clarify the aim of the experiment by asking, "What was the question or hypothesis being investigated?" This will be required to adjust the raw results to the appropriate format and settings for further analysis using statistical cytometry software. Make whatever changes are necessary in order to have the data displayed with the relevant settings (e.g. positive cells, negative gates, fluorescence intensity, cell populations etc.).. Find gates. Cells can be grouped or simply observed clustered together on a density plot or contour diagram. The groups often separate depending on their identity. If one group stains very intensely for a particular marker or antibody, it is concluded that the members of that group all have the identity of the specific cell- type, which expresses that marker. It is common to find cells that are positive for more than one of these markers, and these cells are usually an intermediate and denoted as "double-positive.". Look at scattergraphs. The way the groups of cells ...
Multiparameter flow cytometry studies were performed on clinical samples of human bladder tumors to simultaneously analyse DNA content and the expression of surface glycoproteins defined by monoclonal antibodies T16, Om5, T43, and T138. The results of tests performed on 80 samples of bladder irrigations and tumors from 68 patients were correlated with clinical findings at the time of sampling and with disease outcome prospectively (mean follow-up 2 years). Measuring the level of the panurothelial antigen T16 provided more precision in DNA analysis and served as an internal standard to measure the relative expression of the other cell surface antigens studied. The panel of monoclonal antibodies improved the analytical capacity to study the heterogeneity of antigenic phenotypes within individual samples. Aneuploidy frequently correlated with high stage cancers and with a high rate of clinical cancer progression defined as metastasis or death by cancer. However ploidy was not an entirely reliable ...
CD4 counts can be obtained using flow cytometry results in conjunction with absolute lymphocyte counts from a hematology cell counter or can be quantitated on a flow cytometer with the help of fluorescent beads added at a specific volume for comparison. Although laboratories have various choices of instrumentation and methods for quantifying CD4 counts using flow cytometry, the Center for Disease Control (CDC) provides guidelines for standardization and laboratories must ensure accuracy and precision of their methods for CD4 analysis ...
The Inside Stain Kit contains reagents for the fixation and permeabilization of cells for intracellular staining, for example for flow cytometric evaluation. The kit was also optimized to function in combination with MACS® Technology. Typical applications of the Inside Stain Kit include: Intracellular staining of cytokine producing cells for cytokine detection by flow cytometry Intracellular staining of enriched cells, e.g., CD138+ cells for detection of intracellular kappa/lambda light chains, or melanoma cells for detection of Melan A expression Intracellular staining of other cells enriched using MACS Technology - Principat dAndorra
The Flow Cytometry group is a core facility of the Center for Integrated BioSystems at Utah State University. The facility provides instrumentation, personnel, and expertise to assist researchers in flow cytometry and fluorescence-activated cell sorting (FACS) applications. The laboratory is equipped with a BD Biosciences Special Order FACSAria™ II, which is a high-speed FACS that can perform high-resolution, multicolor flow cytometry analysis. Our FACSAria™ II has 4 lasers that can detect up to 13 different colors in addition to the cell sorting functionality, and uses BD FACSDiva™ software for data acquisition and analysis.. For a complete list of available filter sets, lasers, and wavelengths, see our filter worksheet. Services are offered as fee for service (see fee schedule). For more information please contact Dr. Lihong Teng.. ...
Detection of activated integrin aIIbb3 and P-selectin-expression on mouse platelets 106mouse platelets in 25 l Tyrode-Hepes buffer (1 mM CaCl2) were left untreated or stimulated with 10 M adenosine diphosphate (ADP) or 0.2 U/ml thrombin and directly stained with 10 l of a mixure of Antibody A and Antibody B for 15 min. at RT. Samples were filled up with 400 l PBS and analyzed directly. Platelets were gated by FSC/SSC characteristics.. Note: ADP is a relatively weak agonist which stimulates (reversible) integrin IIbb3 activation but virtually no P-selectin expression. In contrast, thrombin is a very strong platelet agonist which induces strong and irreversible integrin IIbb3 activation and P-selectin expression.. Data sheet (PDF). References: 1. Bergmeier W, Schulte V, Brockhoff G, Bier U, Zirngibl H, Nieswandt B. (2002) Flow cytometric detection of activated mouse integrin alphaIIbbeta3 with a novel monoclonal antibody. Cytometry 1;48:80-6.. 2. Phillips DR, Charo IF, Scarborough RM. (1990) ...
Dr Filby obtained his PhD from the National Institute for Medical Research (NIMR) in Mill Hill, London where he studied the role of the Src family kinases Lck and Fyn in T cell function. He is currently head of the Flow Cytometry Core Facilit (FCCF) at Newcastle University leading a dedicated team of flow cytometry specialists with the sole aim of providing a comprehensive, cutting edge cytometry resource to the wider research community at Newcastle University and beyond. A significant part of his focus is the development of novel cytometry-based techniques that have underpinned several high profile publications in journals including Science and Cell. He also received the Cytometry Part A paper of the year accolade in 2011. He specialises in Imaging Flow Cytometry and the use of fluorescence dyes to track cell proliferation. Dr Filby is also an International Society for the Advancement of Cytometry (ISAC) Shared Resource Laboratory Emerging Leader (SRL-EL) and is heavily involved in a number of ...
We used flow cytometry to investigate the percentage of IL-17-expressing blood T cells ex vivo in 49 healthy controls. Nonadherent PBMCs were stained for CD3, CD4, CD8, and IL-17. No IL-17-producing T cells were detected in the absence of activation (unpublished data). Upon activation with PMA-ionomycin, the percentage of CD3-positive cells producing IL-17 ranged from 0.06 to 2% (Fig. 1, A and B). The vast majority (,90%) of IL-17-positive cells were CD4-positive and CD8-negative (unpublished data). Thus, within the general population, there is considerable interindividual variability in the numbers of IL-17-producing cells present among freshly isolated T cells activated ex vivo. This makes it difficult to assess the impact of genetic lesions on the development of IL-17-producing T cells. We tested nine patients with null mutations in IRAK4 or MYD88, whose cells were unresponsive to IL-1β (and most TLRs and other IL-1 cytokine family members). The proportion of IL-17-producing T cells was not ...
The Invitrogen™ eBioscience™ Super Bright polymer dyes represent a suite of bright fluorophores excited by the violet laser (405 nm). Optimized for use in flow cytometry, the Super Bright dyes allow for expanded use of violet laser excitation and promote streamlined multicolor panel design. This scientific poster describes the use of these dyes and the Invitrogen ™ Attune™ NxT Flow Cytometer to &
The Flow Cytometry facility of the Hellenic Pasteur Institute (HPI) is equipped with a FACS ARIA cell sorter and a FACS Calibur flow cytometer. The facility is supported by the junior researcher Despina Smirlis specialising in the field of Molecular Microbiology and the Research Assistant Dr. Evangelia Xingi. Aim of the facility is to provide advanced flow cytometry and sorting facilities to the HPI scientists as well as to visiting scientists.Flow cytometry is an automated, quantitative and dynamic and multi-parameter way of analysing the physical and chemical properties of particles and cells, based on light scattering by the particles or fluorescence emmission. A flow cytometer is characterized as a successful combination of an haematological analyzer and a fluorescence microscope, that uses the last microscopy field achievements and biochemical analysis as well as computer evolution. ...
Fact.MR has adopted multi-disciplinary approach to shed light on the evolution of the global Flow Cytometry market during the historical period of 2014 - 2019. The study presents a deep-dive assessment of the current growth dynamics, major avenues in the estimation year of 2020, and key prospects over the forecast period 2020 - 2025.. On the back of these aforementioned factors, the global flow cytometry market is expected to grow at a staggering CAGR of 11.4% during the forecast period (2020-2025). It shall surpass a valuation of US$ 8.1 Bn by 2025-end. Increasing usage of image flow cytometers in clinical applications is also expected to boost the flow cytometry market in the future. Extensive rounds of primary and a comprehensive secondary research have been leveraged by the analysts at Fact.MR to arrive at various estimations and projections of the Flow Cytometry market, both at global and regional levels. The analysts have used numerous industry-wide prominent business intelligence tools to ...
Background Multiparameter flow cytometric analysis of bone marrow and peripheral blood cells has ... syndromes . However, the usefulness of flow cytometry for the detection of megakaryocytic and platelet .... Research Article last updated 06/15/2012 - 2:25pm.. ...
This unit describes the use of a novel violet‐excited membrane‐binding probe F2N12S [4′‐N,N‐diethylamino‐6‐(N,N,N‐dodecyl‐methylamino‐sulfopropyl)‐methyl‐3‐hydroxyflavone] for the flow cytometric detection of the changes in membrane asymmetry, fluidity, and charge that accompanies apoptosis
A large body of literature indicates that ESAT-6 and CFP10 induce potent immune responses to protect against M. tuberculosis infection in animal models. In contrast, secretion of these proteins by the ESX-1 specialized secretion system is associated with mycobacterial virulence and pathogenicity. Because these proteins mediate critical host-pathogen interactions, it is important to understand the full range of their biological effects.. In this study, we made the surprising finding that higher concentrations (1.6-3.3 μM) of ESAT-6 inhibited IFN-γ production by M. tuberculosis-responsive T cells in PBMC, and suppressed both proliferation and IFN-γ production by purified CD3+ T cells stimulated with anti-CD3 and anti-CD28, indicating that these effects were independent of APCs. A direct effect of ESAT-6 on human T cells was also suggested by flow cytometry studies that demonstrated binding of ESAT-6 but not CFP10 to CD4+ and CD8+ T cells. Further, ESAT-6 inhibited expression of T cell ...
This studys initial objective was to assess pancreatic β-cell numbers and physiology during immune-mediated islet destruction by following the insulin-positive-to-glucagon-positive cell ratios and other parameters made possible by advanced flow cytometry techniques. We were surprised to observe that the relative frequency of the two endocrine subsets consistently pointed to an unexpected depletion of α-cells, along with the expected β-cell loss at diabetes onset. It is important to point out that because the flow technique lacks an internal reference standard, one cannot accurately determine the absolute number of α- or β-cells in the pancreas, only their relative proportion. We therefore turned to immunofluorescence microscopy and quantitative immunohistochemistry, supported by qRT-PCR, to provide additional, overlapping, and independent techniques. As discussed in our results, all studies supported our initial conclusion that while β-cells are being depleted during autoimmune diabetes, ...
The potentiation of the cytotoxicity of 6-TG by MMR is associated with induced chromosomal rearrangements and growth arrest (10 , 20 , 21) , although the precise nature of the signal generated by MMR and how the signal is transduced is unknown. To study response to 6-TG in the hMLH1-expressing cell lines more fully, we used flow cytometry to analyze the cell cycle profile of MEFs cultured in different doses of 6-TG. In a dose- and time-dependent manner, the wild-type MEFs showed a significant increase in the 4N population, consistent with 6-TG-induced G2 cell cycle arrest (Fig. 3A) ⇓ . G2 cell cycle arrest was followed 1 day later by significant cell death, possibly by apoptosis as evident by the appearance of cells with a DNA content less than that of G1 (Fig. 3A) ⇓ . In contrast to the response of the wild type cells, the mMlh1-deficient cell line CT-5 did not show a specific G2 arrest at any dose tested, including doses where the survival of the cells was reduced dramatically (Fig. 3B) ...
Background Fluorescent Cell Barcoding is a flow cytometry technique that allows you to answer a larger number of questions in an...
3., determination of bone-turnover markers using immunoassays and molecular biological methods.. Serum tumor marker determinations. The majority of these tests (11 analytes: PSA, free PSA, CEA, AFP, HCG-β, CA125, CA15-3, CA19-9, CA72-4, Cyfra 21-1, NSE) are measured by a Roche MODULAR E170 analyzer. Two markers (Thyreoglobulin and TPA) are performed by the Immunochemistry Division, while Calcitonin is measured by the Endocrinology Division of the Department. The interest in the serum tumor marker determination is increasing constantly since the foundation of the division. The number of tests was 8,500 in 2000 while 61,000 in 2009. Due to the increasing number of tests we changed the frequency of measurement and provide results each day of a week.. Molecular Oncology. The diagnostic tests using molecular biological methods can be grouped into two major profiles. The first group of assays - together with the flow cytometric determinations - serves the diagnostics and follow-up of the ...
[108 Pages Report] Check for Discount on Global Multi-parameter Monitoring Market Professional Survey Report 2017 report by QYResearch Group. This report studies Multi-parameter Monitoring in Global market, especially...
Fully automated 5-part differential haematology analyser, benchtop model. Also for standardised body fluid analysis. The XT-4000i uses Sysmexs unique fluorescence flow cytometry, which means it looks at RNA/DNA content, cell size and inner cell complexity rather than just cell size - for great differentiation results.
Neuman Room 1-6823 Tim Mosmann and Gaurav Sharma The Bioinformatics cluster meets monthly for student and faculty talks on current research in the area of Bioinformatics. The meeting is open to all members of the University community. Tim Mosmann, Center for Vaccine Biology and Gaurav Sharma, Electrical & Computer Engineering are discussing "SWIFT: High-resolution High-dimensional Analysis of Flow-Cytometry Data - Clustering, Templating, and Competition." Lunch will be provided from the generous support of UCIS.. Links: ...
In this report, we characterize the novel human cell cycle gene, Spy1. Akin to its homologous Xenopus relative, Spy1 induces maturation of Xenopus oocytes upon microinjection. In mammalian cells, we demonstrate that Spy1 is a regulator of cell cycle progression. Overexpression of Spy1 increases proliferation in several mammalian cell lines, as indicated by cell growth curves. This may be explained by the ability of Spy1 to bind to and prematurely activate the G1/S kinase, cdk2. In support of this, flow cytometry studies using 293T cells demonstrate that Spy1 decreases the overall population of cells in G1 phase of the cell cycle. Furthermore, cells overexpressing Spy1 demonstrate an enhancement in the incorporation of BrdU and in MTT activity, respectively, indicating that both DNA synthesis and mitochondrial enzyme activity are enhanced. Taken together, Spy1 appears to be a potent regulator of cell cycle progression.. Previous studies demonstrated that the Xenopus Spy1 homologues, X-Spy1 and ...
Flow cytometers associated computers, controls instrument set-up and sample acquisition through specialised softwares. As data is acquired, a file of data, often referred as flow cytometry standard (fcs) data file is created. The computer program can then be used to analyse data subsequent to its acquisition or to export the data.. BD FACSDivaTM software controls the efficient setup and acquisition of flow cytometry data from the BD LSRFortessa and the BD FACSAria Fusion sorter. Acquisition in the BD Accuri C6 is controlled by the Accuri C6 software, while sorting in the FACSMelody is performed by the very interactive BDFACSChorus platform.. ...
While meta-analysis has demonstrated increased statistical power and more robust estimations in studies, the application of this commonly accepted methodology to cytometry data has been challenging.
van der Pol E, Coumans FAW, Grootemaat AE, Gardiner C, Sargent IL, Harrison P, Sturk A, van Leeuwen TG, Nieuwland R. Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost 2014; 12: 1182-92.. Sarah E. Headland, Hefin R. Jones, Adelina S. V. DSa, Mauro Perretti & Lucy V. Norling Cutting-Edge Analysis of Extracellular Microparticles using ImageStreamX Imaging Flow Cytometry. Nature Scientific Reports 4 : 5237 DOI: 10.1038 June 10 2014.. Erdbrügger U, Rudy CK, E Etter M, Dryden KA, Yeager M, Klibanov AL, Lannigan J. Imaging flow cytometry elucidates limitations of microparticle analysis by conventional flow cytometry. Cytometry A. 2014 Sep;85(9):756-70. doi: 10.1002/cyto.a.22494. Epub 2014 Jun 5.. ...
FC allows correlation of up to six different markers on a single cell and is much faster than immunohistochemistry (IHC), which can only analyze one marker per slide. In addition, FC can be used on peripheral blood, bone marrow aspirate, and cerebrospinal fluid - samples that are not able to be analyzed by IHC. Overall, FC is less expensive and much faster (one to two lab hours) to process than IHC, although the interpretation by a skilled technician remains.. The one disadvantage of FC is that the architecture of a sample is lost as the cells are analyzed in a suspension, making this method less desirable than tumor sections or slides in solid tumors where the architecture of the tumor is important in staging.. Because this new technology detects very low levels of B-cell leukemic cells, it will enable clinicians to cease therapy with a level of clinical certainty unheard of in the recent past, thus potentially preventing unnecessary courses of expensive therapy. Common current therapy includes ...
The Immune Response in PLP-Overexpressing Mice Chi Wang Ip, Antje Kroner, Martin Bendszus, Christoph Leder, Igor Kobsar, Stefan Fischer, Heinz Wiendl, Klaus-Armin Nave, and Rudolf Martini. (see pages 8206-8216). This week, Ip et al. examined mice that overexpress the myelin component proteolipid protein (PLP) and display late-onset demyelination. The authors report that primary glial damage caused a secondary immune response that contributes to the pathology. CD8-expressing T-lymphocytes were upregulated in the PLP transgenic mice, and the T-cells were closely associated with major histocompatibility complex I (MHC-I). The authors surmised that MHC-I+ mutant oligodendrocytes were targeted by T-cells. Flow cytometry experiments confirmed that the brain CD8+ cells were activated mature effector cells. The authors crossed the PLP mice with mice deficient for recombination activating gene-1 (RAG-1) that lack mature T- and B-lymphocytes. Demyelination was reduced in these mice, an effect that was ...
Holme, A.L., Pervaiz, S., Yadav, S.K. (2007). Automated laser scanning cytometry: A powerful tool for multi-parameter analysis of drug-induced apoptosis. Cytometry Part A 71 (2) : 80-86. [email protected] Repository. https://doi.org/10.1002/cyto.a. ...
Christine Probst holds her masters and bachelors degrees in bioengineering. She currently works as an application scientist at Merck where she develops new assays using Amnis® imaging flow cytometry. For the past 4 years, her primary focus has been analysis of sub-visible particles in therapeutic protein and vaccines using imaging flow cytometry. In this presentation, Christine will discuss how imaging flow cytometry addresses limitations of current particle analysis techniques, and will present several case studies where imaging flow cytometry yielded new insights into therapeutic formulations ...
The innate immune response elicited by activation of TLRs (Toll-like receptors) plays an important role in the pathogenesis of atherosclerosis. We hypothesized that cardiovascular risk factors are associated with the activation status of the innate immune system. We therefore assessed the responsiveness of TLRs on circulating cells in two groups of patients with established atherosclerosis and related this to the presence of cardiovascular risk factors. TNF (tumour necrosis factor)-α release induced by TLR2 and TLR4 activation was measured in patients with established coronary [PCI (percutaneous coronary intervention) study, n=78] or carotid artery disease [CEA (carotid endarterectomy) study, n=104], by stimulating whole blood samples with lipopolysaccharide (TLR4 ligand) and Pam3CSK4 [tripalmitoylcysteinylseryl-(lysyl)4; TLR2 ligand]. As an early activation marker, CD11b expression was measured by flow cytometry on CD14+ cells. Obesity was the only risk factor that correlated with the TLR ...
Product list of China Flow Cytometry Device, show the variety of China products related to Flow Cytometry Device; You can choose the right product of China Flow Cytometry Device on this list.
Although HeLa cells served as an established model for the initial assessment of EHD1 in β1 integrin trafficking (Becker and Hannun, 2003; Powelka et al., 2004), integrin function has been studied more extensively in fibroblasts. Using MEF cells derived from mice lacking EHD1 expression (Rapaport et al., 2006), we examined the role of EHD1 in regulating the trafficking of β1 integrins. Loss of EHD1 led to an accumulation of intracellular β1 integrins observed after 2 hours of chase, when compared with β1 integrins in normal Ehd1+/+ MEF counterpart cells. Moreover, after 4 hours of chase, an approximately twofold increase was observed in the level of non-recycled β1 integrins. The role of EHD1 in regulating β1 integrin recycling was further supported by flow cytometry experiments with the MB1.2 antibody (which recognizes conformation-independent β1 integrins), demonstrating that overall levels of β1 integrins are reduced on the cell surface as a result of impaired recycling (Fig. 4B). To ...
Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer 600mL Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer Flow Cytometry...
Mitochondria are an important source of superoxide production contributing to physiological and pathological responses, including vascular oxidative stress that is relevant to cardiovascular diseases
PROBLEM: To address the hypothesis that pre-eclampsia (PE) impacts the fetal immune system, we investigated the prevalence of distinct immune cell subsets along with plasma cortisol and cytokine levels in pre-term newborns of PE mothers. METHOD OF STUDY: Cord blood and peripheral blood samples on the 1st, 3rd and 7th postnatal days of life were collected from 14 pre-term infants affected by PE and 14 non-PE pregnancies. We measured plasma cortisol and cytokine levels with immunoassays and assessed the prevalence of T, NK and DC subsets using flow cytometry. RESULTS: The prevalence of CD4+ cells was lower in PE infants, while that of memory T cells was higher. Myeloid DCs had a lower prevalence in PE neonates. Cytokine and cortisol levels were lower in PE neonates. CONCLUSION: Our observations show that PE pregnancies are associated with altered newborn immune status during the first week of life.. ...
Perform sample processing and run multicolor flow cytometry panels for clinical research sample testing with an emphasis on sample processing, assay procedure, instrument operation, troubleshooting and data analysis.. Follow this link for more information.. ...
THP-1 cell surface markers for Flow Cytometry - posted in Flow Cytometry: I am working with THP-1 cells (ATCC TIB 202), a human monocytic cell line. The majority of people that use this cell line are interested in the differentiated macrophage phenotype, which can be achieved by stimulating THP-1 cells with PMA or Vitamin D. I mainly use these cells in the undifferentiated form without any type of stimulation. I am planning on doing some cell surface expression of various mole...
Sigma-Aldrich offers abstracts and full-text articles by [Valentina Salzman, Valentina Porro, Mariela Bollati-Fogolín, Pablo S Aguilar].
MRD in AML can be based on molecular methods (PCR targeting fusion genes, mutations or expression levels of appropriate genes) or on multicolour flow cytometry. In flow cytometric MRD analysis leukemia-associated immunophenotypes (LAIPs) are indentified at diagnosis and applied to follow-up samples. Earlier, with four to six colour combinations, patient-specific LAIPs were designed on the basis of antigen profiles at diagnosis. Our strategy with eight to ten colour flow cytometry is to apply four standard combinations (for granulocytes, monocytes, stem cells and aberrant markers, respectively). This strategy not only allows the identification of LAIPs but also the analysis of granulocytic and monocytic differentiation patterns. The combinations also contain markers to identify e.g. mast cells, plasmacytoid dendritic cells, basophils and plasma cells, which may contaminate the so called blast area in CD45/side scatter plots. We in Tampere have three-year experience of ten-colour flow cytometric ...
In an effort to objectively assess TILs, we have developed an automated, reproducible method for in situ measurement of lymphocyte infiltrate, quantifying expression of up to three TIL subpopulations within the tumor and adjacent stroma. We validated this method by comparison with flow cytometry on tonsil, and demonstrated reproducibility on breast tissue. For breast tumors before neoadjuvant treatment, high stromal expression of CD3, CD8, and CD20 all predicted pCR in univariate analysis. Moreover, CD20 predicted pCR in multivariate analysis independently of age, tumor size, nuclear grade, nodal metastasis, ER, PgR, and HER2 status, and Ki-67 AQUA score. Agreement between this automated objective assay and traditional semiquantitative pathologist estimates is very good.. This is the first study to significantly correlate CD20 expression with response to neoadjuvant chemotherapy in both univariate and multivariate analysis. Previously, this association has been equivocal. Although one study ...
Discovery accesses a deep reservoir of specimens, facilities, technologies, and expertise to equip our researcher clients with large-scale, custom services in flow cytometry. Our scientists are accomplished in designing and executing flow cytometric analyses and provide custom consultations to optimize project results. Our team also can aide in panel design, such as the custom validation of fluorophores and antibody clones for your assays. Our scientist analyze and report data outputs. Sophisticated technologies are used in our flow cytometry laboratory; these have high-throughput and automation capabilities to handle large-scale projects. Our primary cell processing facility incorporates a dedicated, separate flow cytometry laboratory. ...
PPD® Laboratories flow cytometry assays integrate into our bioanalytical and central labs. This integration provides flexibility to leverage our operational and organizational structures for a tiered regulatory approach to meet clients needs.. From fit-for-purpose assays to projects that require GCLP-, GLP- or CLIA-compliant assays we have the technology platforms, the clinical expertise and the collaborative and flexible mindset needed to develop customized solutions for our clients.. We have a dedicated team of scientists at the bioanalytical lab and many cross-trained scientists across the various central lab locations. Research and development for flow cytometry assays is performed in our Richmond, Virginia, location, where our scientists have more than 25 years of combined experience.. ...
Flow Cytometry or Fluorescence Activated Cell Sorting (FACS) as a technique was developed in the 1960s and allowed sorting and collection of viable cells from a heterogeneous mixture. The first FACS systems used a mercury arc light source and could measure just one parameter, fluorescence intensity. Data archival was primitive by todays standards and instruments could process 75-100,000 cells per minute. With the advent of computing, data storage and analysis technologies along with significant developments in lasers and optical technologies in the decades that ensued, flow cytometers have become the mainstay of the modern day clinical laboratory. Cytometry has found applications in fields as diverse as molecular biology and immunology to marine science. These instruments can now process millions of cells per minute. There are three main systems within the Flow Cytometry instrument. A fluidics system transports sample particles in the fluid stream to the light source. The optical system is the heart of
Degranulation assay of derived MCs.A. A representative result of flow cytometry on original CD34+ hematopoietic precursors using anti-FcεRI and anti-CD117 anti
ProPure® rProtein G Rapid Flow is an affinity medium coupled with Biomedal rProtein G (3xC) and designed for immunoglobulin capture and purification. ProPure® rProtein G Rapid Flow medium is compatible with chromatography systems such as ÄKTA™ or FPLC™, providing high binding capacity and a cost-efficient purification process.. ...
ProPure® rProtein A Rapid Flow is an affinity medium coupled with Biomedal rProtein A (4xZ) and designed for immunoglobulin capture and purification. ProPure® rProtein A Rapid Flow medium is compatible with chromatography systems such as ÄKTA™ or FPLC™, providing high binding capacity and a cost-efficient purification process ...
Figure 2. Flow cytometric characterization of γδ T cells in our patient demonstrates specific expansion of a homogeneous Vδ2+ T cell population. Flow cytometry performed on frozen PBMC either unstimulated for surface markers or PMA/ionomycin/brefeldin A stimulated for intracellular molecules. (A) FACS used to identify live, singlet lymphocytes (not shown), which were gated on to identify CD3+ T cells in the patient and a representative IFX-treated control (HC). CD3+ cells further gated on to identify Vδ1+/Vδ2+ T cells. The indicated plots display markers specific to the Vδ2+ T cell population with gates based on FMO control (not shown). (B) Mass cytometry used to identify live, singlet, CD3+CD14-CD19-TCRγδ+ cells in the patients PBMC for expression of the indicated markers. (C) viSNE analysis performed using 36 markers to cluster PBMC populations in the patient and 5 age-matched male patients with AS. CD3ε, CD4, CD8α, CD14, CD19, CD56, and TCRγδ antigens were used to gate indicated ...
Cytokine flow cytometry (CFC) provides a multiparameter alternative to ELISPOT assays for rapid quantitation of antigen-specific T cells. To increase the throughput of CFC assays, we have optimized methods for stimulating, staining, and acquiring whole blood or PBMC samples in 96-well or 24-well plates. We have developed a protocol for whole blood stimulation and processing in deep-well 24- or 96-well plates, and fresh or cryopreserved peripheral blood mononuclear cell (PBMC) stimulation and processing in conventional 96-well round-bottom plates. Samples from both HIV-1-seronegative and HIV-1-seropositive donors were tested. We show that the percent response, staining intensity, and cell recovery are comparable to stimulation and processing in tubes using traditional methods. We also show the equivalence of automated gating templates to manual gating for CFC data analysis. When combined with flow cytometry analysis using an automated plate loader and an automated analysis algorithm, these plate-based
Flow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradient centrifugation and evaluated for their antigen specificity or reactivity towards a vaccine. Here, we describe staining of bovine lymphocytes with peptide (p)-MHC class I tetramers and antibodies specific towards cellular activation markers for evaluation by multiparametric flow cytometry, as well as interferon (IFN)-γ ELISpot and cytotoxicity using chromium (51Cr) release assays. A small component on the use of immunoinformatics for fine-tuning the identification of a minimal CTL epitope is included ...
Definition : Reagents used in cytologic assays performed by flow cytometers. They include labels for cell membranes and standards to normalize instrument setup, cell count, and proliferation. These reagents are frequently available in kits that allow the calibration, control, and standardization of flow cytometry analysis.. Entry Terms : "Antibodies, Monoclonal, Flow Cytometry" , "Flow Cytometry Reagents" , "Reagents, Lymphoma Cell Immunophenotype" , "Reagents, Lymphocyte T/B Cell Subpopulation" , "Reagents, Lymphocyte T/B Cell Population" , "Reagents, Lymphocyte T Cell Population" , "Reagents, Flow Cytometry" , "Reagents, Cytology/Histology, Flow Cytometry". UMDC code : 17052 ...
In a recent unit in this series, protocols for the isolation, cryopreservation, thawing, and immunophenotyping of HPBMC isolated from peripheral whole blood using cell surface marker (CSM) staining and multi‐color flow cytometry analysis were presented
the right answer, the first time, with the least variability.. We provide a wide variety of human immunology and mouse immunology services. These include up to 20-color cell subset phenotyping and intracellular staining, lymphoproliferation assays, ELISPOTs, and multiplexed cytokine determination.. We also provide operator-assisted cell sorting (FACS) and round-the-clock access to a variety of flow cytometers.. Quality control is a priority of our Shared Resource as is proficiency testing of our Technologists. We continuously conduct proficiency testing and participate in multiple proficiency testing programs.. DartLab staff are available for consultation and assistance with planning, execution, analysis and interpretation of immunological assays and multi-color flow cytometry.. We encourage users to become self-sufficient and have an active teaching and training program, including a 5-lecture flow cytometry course taught by DartLab staff.. In addition, our services are available to Pharma for ...
Lets take a look at some key factors in choosing flow cytometry antibodies ! Choosing high-quality and reliable fluorescent antibodies ensures the smooth running of flow cytometry and data analysis, especially multi-color flow cytometry that needs high resolution for color compensation.
In an observational study,AF was associated with a homogeneously elevated CV risk marker profile in HFrEF patients, whereas in HFpEF, the presence of AF was associated with a more scattered risk marker profile, indicating different underlying pathophysiological mechanisms.
Flow Cytometry is a rapidly developing and amazingly flexible technology that combines many of the advantages of microscopy and biochemical analysis to allow fast, sensitive and reproducible multiparametric analyses at the single cell level. It has introduced new horizons in the identification and characterization of phenotypically heterogeneous cell populations in a wide range of research areas. Cell Sorting is an advanced key technology in Flow Cytometry and a fundamental tool in medical research, which allows rapid analysis and accurate collection of specific cell populations at extremely high speed, high purity and high viability for further research or clinical purposes. ...
TY - JOUR. T1 - The prognostic value of multiparametric flow cytometry in AL amyloidosis at diagnosis and at the end of first-line treatment. AU - Muchtar, Eli. AU - Jevremovic, Dragan. AU - Dispenzieri, Angela. AU - Dingli, David M. AU - Buadi, Francis K.. AU - Lacy, Martha. AU - Gonsalves, Wilson. AU - Hayman, Suzanne R.. AU - Kapoor, Prashant. AU - Leung, Nelson. AU - Russell, Stephen J. AU - Lust, John A.. AU - Lin, Yi. AU - Go, Ronald S.. AU - Chakraborty, Rajshekhar. AU - Zeldenrust, Steven. AU - Kumar, Shaji K. AU - Kyle, Robert A.. AU - Rajkumar, S Vincent. AU - Gertz, Morie. PY - 2017/1/5. Y1 - 2017/1/5. N2 - Multiparametric flow cytometry (MFC) in amyloid light-chain (AL) amyloidosis has not been widely adopted and, consequently, there is little information on its clinical relevance. We studied 173 patients with AL amyloidosis who underwent MFC immunophenotyping of bone marrow sample at diagnosis and 82 patients at the end of the first line of treatment (EOT). The number of monotypic ...
Epirubicin (EPI) is one of the most widely used anticarcinogens; however, serious side effects, including cardiomyopathy and congestive heart failure, limit its long-term administration. To overcome this problem, the HAIYPRH peptide ligand was used with EPI in the synthesis of a HAIYPRH-EPI conjugate. The anticancer activity and cellular uptake of the conjugate were measured and evaluated. The results of the present study indicated that the cytotoxicity of HAIYPRH-EPI was correlated with the expression of the cell surface transferrin receptor (TfR). The conjugate exerted high cytotoxicity and proapoptotic function when in an LN229 glioma cell line, which overexpresses surface TfR. It was hypothesized that transferrin (Tf) can promote cytotoxicity. Conversely, the conjugate exhibited very low cytotoxicity and proapoptotic function in a U87 glioma cell line, in which surface TfR expression was undetectable. In addition, fluorescence microscopy and flow cytometry methods were used to evaluate ...
Article Identification and characterization of bacillus anthracis spores by multiparameter flow cytometry. In response to the need for methods that can rapidly detect potentially virulent Bacillus anthracis spores, we developed a two-color flow cytom...
The number of skin cancers continues to rise, accounting for approximately 40% of all cancers reported in the United States and approximately 9,500 deaths per year. Studies have shown reactive oxygen species (ROS) type free radicals are linked to skin cancer and aging. Therefore, it is important for us to identify agents that have anti-oxidant properties to protect skin against free radical damage. The purpose of this research is to investigate the anti-oxidant properties of bisabolol, silymarin, and ectoin that are components from chamomile, milk thistle, and halophilic bacteria, respectively. We measured the ability of bisabolol, silymarin, and ectoin to modulate the hydrogen peroxide (H2O2)-induced upregulation of ROS free radicals in normal human skin fibroblasts in vitro. Using a flow cytometry-based assay, we demonstrated that varying concentrations of these natural components were able to inhibit upregulation of H2O2-generated free radicals in human skin fibroblasts in vitro. Our results indicate
Introduction Mucosal-Associated Invariant T cells (MAIT) are recently described human T lymphocytes with possible functions in mucosal antibacterial host defence.1 MAIT express a highly conserved T cell receptor (TCR) alpha chain consisting of an invariant Vα7.2-Jα33 rearrangement.2 Recent evidence suggests a role for T lymphocytes in the pathogenesis of chronic pancreatitis (CP), but little is known about the composition of T cell subsets in this disease.3 4 Intestinal bacterial overgrowth is common in chronic pancreatitis patients.5 We hypothesised that this antigenic load may promote MAIT infiltration of the pancreas and used flow cytometry to study T lymphocytes in resected pancreatic tissue and blood of patients with chronic pancreatitis.. ...
The cytokinesis-block micronucleus assay can be employed in triage radiation biodosimetry to determine the dose of radiation to an exposed individual by quantifying the frequency of micronuclei in binucleated lymphocyte cells. Partially automated analysis of the assay has been applied to traditional microscope-based methods, and most recently, the assay has been adapted to an automated imaging flow cytometry method. This method is able to automatically score a larger number of binucleated cells than are typically scored by microscopy. Whole blood samples were irradiated, divided into 2 mL and 200 mL aliquots, cultured for 48 h and 72 h, and processed to generate calibration curves from 0-4 Gy. To validate the method for use in radiation biodosimetry, nine separate whole blood samples were then irradiated to known doses, blinded, and processed. Results indicate that dose estimations can be determined to within ±0.5 Gy of the delivered dose after only 48 h of culture time with an initial blood ...
The global microbial CH4 production is estimated to reach one billion tons annually. Methanogenic archaea produce CH4 in wetlands, rice fields, ruminant and termite digestive systems and have a decisive impact on the planets atmospheric carbon cycle [42]. At the same time, the industrial scale anaerobic digestion of biomass to CH4 plays a vital role in the future global energy mix. All methanogenic archaea capable of CO2 reduction contain the cofactor F420 as an integral part of the methanogenic pathway. In this study, F420 autofluorescence was tested as a universal marker for methanogenic archaea. Genes encoding for F420 biosynthesis enzymes were identified in 653 bacterial and 173 archaeal species [43]. Non-methanogenic but F420 containing microorganisms have reported F420 concentrations of about one fortieth of the concentrations in hydrogenotrophic methanogenic archaea [19], which is below detection limit of the developed protocol. For the methanogenic archaea, however, the F420 cofactor ...
TY - JOUR. T1 - Flow Cytometry and T-Cell Response Monitoring after Smallpox Vaccination. AU - Poccia, Fabrizio. AU - Gioia, Cristiana. AU - Montesano, Carla. AU - Martini, Federico. AU - Horejsh, Douglas. AU - Castilletti, Concetta. AU - Pucillo, Leopoldo Paolo. AU - Capobianchi, Maria Rosaria. AU - Ippolito, Giuseppe. PY - 2003/11. Y1 - 2003/11. N2 - Orthopoxvirus zoonosis or smallpox as result of bioterrorism or biological warfare represents a risk for epidemic spread. By monitoring T-cell responses by flow cytometry, we observed a recall response after recent vaccination against smallpox. When the high similarity between the orthopoxviruses is considered, this rapid assay that uses vaccinia antigens could identify recently exposures.. AB - Orthopoxvirus zoonosis or smallpox as result of bioterrorism or biological warfare represents a risk for epidemic spread. By monitoring T-cell responses by flow cytometry, we observed a recall response after recent vaccination against smallpox. When the ...
TY - JOUR. T1 - Invariant natural killer T cells direct B cell responses to cognate lipid antigen in an IL-21-dependent manner. AU - King, Irah L.. AU - Fortier, Anne. AU - Tighe, Michael. AU - Dibble, John. AU - Watts, Gerald F.M.. AU - Veerapen, Natacha. AU - Haberman, Ann M.. AU - Besra, Gurdyal S.. AU - Mohrs, Markus. AU - Brenner, Michael B.. AU - Leadbetter, Elizabeth A.. PY - 2012/1/1. Y1 - 2012/1/1. N2 - Mouse invariant natural killer T cells (iNKT cells) provide cognate and noncognate help for lipid and protein-specific B cells, respectively. However, the long-term outcome for B cells after cognate help is provided by iNKT cells is unknown at present. Here we found that cognate iNKT cell help resulted in a B cell differentiation program characterized by extrafollicular plasmablasts, germinal-center formation, affinity maturation and a robust primary immunoglobulin G (IgG) antibody response that was uniquely dependent on iNKT cell-derived interleukin 21 (IL-21). However, cognate help ...
Scientists estimated the genome size for 40% of the species in the genus[6]; using Flow Cytometry. Penstemon digitalis has one ...
Flow cytometry bioinformatics. H. *Hypercycle (chemistry). I. *Inferring horizontal gene transfer. M. *Multi-state modeling of ...
Measured by flow cytometry: Normal values ,2.5% total T cells; ,1% of total lymphocytes in peripheral blood ...
Flow cytometry in veterinary oncology. Reggeti F, Bienzle, D. Vet Pathol. 2011 Jan;48(1):223-35 Utility of polymerase chain ... Flow cytometry detects antibodies linked to tumour cell surface antigens in fluid samples or cell suspensions. Polymerase chain ...
Flow Cytometry and Sorting. Melamed MR, Mendelsohn M & Lindmo T (eds), Alan R. Liss, Inc., New York. pp. 291-314. ISNBM 0-471- ...
Flow cytometry. Methods in Cell Biology. 42. San Diego: Academic Press. pp. 263-74. ISBN 0-12-203052-4. http://www. ...
Flow Cytometry. Supports automated quality control, centralized data management and web-based data sharing. Integrates with ... LabKey Server provides a secure data repository for all types of biomedical data, including mass spectrometry, flow cytometry, ... Cytometry Part A. 73A (9): 847. doi:10.1002/cyto.a.20600. "The Best of Both Worlds: Integrating a Java Web Application with SAS ... "Development of an automated analysis system for data from flow cytometric intracellular cytokine staining assays from clinical ...
Analysis by flow cytometry and fluorescence microscopy". Journal of Immunological Methods. 133 (1): 87-97. doi:10.1016/0022- ... "Determination of lymphocyte division by flow cytometry". Journal of Immunological Methods. 171 (1): 131-7. doi:10.1016/0022- ... Lyons AB, Doherty KV (February 2004). "Flow cytometric analysis of cell division by dye dilution". Current Protocols in ... Ueckert JE, Nebe von-Caron G, Bos AP, ter Steeg PF (October 1997). "Flow cytometric analysis of Lactobacillus plantarum to ...
... is a very common flow cytometry test in which fluorophore-conjugated antibodies are used as probes for ... Young, Yoon Kow; Bolt, Alicia M.; Ahn, Ryuhjin; Mann, Koren K. (2016). "Analyzing the Tumor Microenvironment by Flow Cytometry ... An example of information provided through Immunophenotyping: "The flow cytometric immunophenotyping report indicated the ... The labelled cells are processed in a flow cytometer, a laser-based instrument capable of analyzing thousands of cells per ...
Flow cytometry Dynabeads. ... flow through. With this method, the cells can be separated ... S. Miltenyi, W. Muller, W. Weichel, and A. Radbruch, "High Gradient Magnetic Cell Separation With MACS," Cytometry, vol. 11, no ...
Practical Flow Cytometry. John Wiley & Sons. pp. 480-. ISBN 978-0-471-43403-0. " Hematologic Diagnostics Go with the Flow". ... Flow Cytometry in Clinical Diagnosis. ASCP. pp. 308-. ISBN 978-0-89189-625-8. "Low-cost neonatal test may save many babies". 26 ... Flow Cytometry, Hemostasis and Thrombosis, Molecular Diagnostics, Hematology Informatics, Hemoglobinopathies, Hemolytic Anemias ... In 2001 the organization published the first flow based reference method for platelet counting. In 2005 it published the ...
"Flow Cytometry Basic". Archived from the original on September 20, 2013. Retrieved 2012-11-17. CS1 maint: Unfit url (link) ... "flow karyotyping" of cells. In flow karotyping, isolated metaphase chromosomes are stained and measured in a flow ... Another use of microfluorometry is flow cytometry which uses the emission of fluorochrome molecules and usually a laser as a ... For example, E. coli bacteriophages lambda and T4 were able to be separated by flow cytometry which allowed for genomic ...
Flow cytometry is a diagnostic tool in order to count/visualize the amount of lymphatic cells in the body. T cells, B cells and ... Landay AL, Muirhead KA (July 1989). "Procedural guidelines for performing immunophenotyping by flow cytometry". Clin. Immunol. ... NK cells are nearly impossible to distinguish under a microscope, therefore one must use a flow cytometer to distinguish them. ...
Founding Director of the NCI-funded National Urinary Bladder Flow Cytometry Network which established clinical flow cytometry ... "Flow cytometric evaluation of bladder cancer: recommendations of the NCI flow cytometry network for bladder cancer". World ... Coon JS, Weinstein RS, (ed): Diagnostic Flow Cytometry. Williams and Wilkens, Co., Baltimore, pp. 1-199, 1991 Bacus, JW; Wiley ... urinary bladder flow cytometry; image analysis; holographic microscopy; robotic telepathology; quantitative ...
Givan, Alice L. (2011-01-01). "Flow cytometry: an introduction". Methods in Molecular Biology (Clifton, N.J.). 699: 1-29. doi: ... Bone marrow studies including aspiration, Flow-cytometry, Cytogenetics, Fluorescent in situ hybridisation and molecular studies ...
Flow Cytometry First Principles. p. 6. "Applied Energy Program". Los Alamos National Laboratory. Retrieved January 31, 2017. " ... The laboratory contributed to the early development of the flow cytometry technology. In the 1950s, researcher Mack Fulwyler ... which produces a laminar flow of liquid that breaks up into separate, fine drops. In 1969, Los Alamos reported the first ...
Larry A. Sklar (2 September 2005). Flow Cytometry for Biotechnology. Oxford University Press, USA. pp. 218-219. ISBN 978-0-19- ...
... a flow cytometry laboratory; a bioreactor facility; and a laboratory focused on the oceanic deep biosphere. Center for Ocean ... MacIsaac Facility for Aquatic Cytometry. The Laboratory was established by Drs. Charles and Clarice Yentsch in 1974 as a ...
Ultra-High Performance Flow Cytometry. Financial support of Atlantic Philanthropies, the Government of Queensland and The ...
"Flow Cytometry Intracellular Staining Guide". eBioscience, Inc. Retrieved 2011-09-25. "Croton Oil". OilHealthBenefits. ...
"Flow Cytometry Intracellular Staining Guide". eBioscience, Inc. Retrieved 2011-09-25. Cohen, Adina; Brodie, Chaya; Sarid, Ronit ...
ISBN 0-683-30751-7. Lecoeur H (2002). "Nuclear apoptosis detection by flow cytometry: influence of endogenous endonucleases". ...
However, flow cytometry is the only currently used technique able to determine the sex of future progeny by measuring DNA ... Sperm sorting by flow cytometry is an established technique in veterinary practice, and in the dairy industry most female cows ... Flow cytometry is another method used to sort sperm and adaptations of this technique opens new opportunities in sperm sorting ... However, because flow cytometry-based sperm sorting often uses fluorescent dyes that often stain DNA, the safety of this ...
An optimized method for routine HLA-B27 screening using flow cytometry. Cytometry, 1994; 18:21-29. K. Strauss, Insulin ... A comparative study of DNA content as measured by flow cytometry and image analysis in 1864 specimens. Analytical Cellular ... Cytometry. 1999 October 15;38(5):231-7. V. Chernyshov, E. Vykhovanets, I. Slukvin, Y. Antipkin, A. Vasyuk, K. Strauss, Analysis ... Cytometry, 1996; 26:52-59. B. Bertino, W. Knape, M. Pytlinska, K. Strauss, J-C. Hammou. ...
... conjugated with phycoerythrin to label IgG or IgM-coated RBCs and enhance the detection by flow cytometry The flow cytometry is ... Flow Cytometry is the method of choice. By examining markers on the surface of blood cells, the method can determine whether ... Due to their small size, PFCs are able to permeate circulation where erythrocytes may not flow. In tiny capillaries, PFCs ...
CS1 maint: Extra text: authors list (link) R.C. Sobti , Awtar Krishan (Editors) (2003). Advanced Flow Cytometry: Applications ...
Instead, plasma cells are identified through flow cytometry by their additional expression of CD138, CD78, the Interleukin-6 ...
"Flow visualization and flow cytometry with holographic video microscopy". Optics Express. 17 (15): 13071-13079. Bibcode: ... Flow cytometry and particle tracking and characterization. Images created by digital holography are calculated from the ... Kemmler M; Fratz M; Giel D; Saum N; Brandenburg A; Hoffman C (2007). "Noninvasive time-dependent cytometry monitoring by ...
The Flow and Image Cytometry Laboratory provides the University of Oklahoma research community with state-of-the-art cell ... The Laboratory for Molecular Biology and Cytometry Research is a state of the art facility offeri... ...
... the most common examples are flow cytometry and image cytometry, which are primarily optical methods. Flow Cytometry. Flow ... and is used to measure many of the same parameters as flow cytometry. Image cytometry, however, carries the added ability of ... Hallmarks of flow cytometry are analysis speed, detection sensitivity, the ability to measure many parameters simultaneously, ... Recent trends in flow cytometry technology include single cell spectroscopy, single cell mass spectrometry, imaging of ...
Flow Cytometry. Current applications in flow cytometry extend far beyond traditional lymphocyte immunophenotyping. Flow ... cytometry is used for such varied applications as cell cycle analysis, telomere length determination, microvesicle analysis, ...
Flow cytometry uses fluorescence as a quantitative tool; the utmost sensitivity of flow cytometry is unmatched by other ... "Microfluidic impedance-based flow cytometry". Cytometry Part A. 77A (7): 648-666. doi:10.1002/cyto.a.20910.. ... Current Protocols in Cytometry, Wiley-Liss Pub. ISSN 1934-9297. *Flow Cytometry in Clinical Diagnosis, v4, (Carey, McCoy, and ... A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high ...
... Recent technological innovations in flow cytometry now allow oceanographers to collect high-frequency ... Time-varying clusters in large-scale flow cytometry Jeremy Hyrkas. , Daniel Halperin. , Bill Howe. . IAAI Conference 2015 * ... Scalable clustering algorithms for continuous environmental flow cytometry Jeremy Hyrkas. , Sophie Clayton. , Francois Ribalet ... We propose the GMM with partitioning approach for classification of large-scale, high-frequency flow cytometry data. ...
... *. Beckman Coulter Cell-Sorter (MoFlo XDP). Location: Department of Dermatology, Experimental Dermatology and ... It unites the possibility of high throughput data acquisition of flow cytometry with morphological information obtained by ... 10-colour flow cytometer, Gallios, Beckman Coulter. Location: Neurology, c/o ICB, Mendelstr. 7 Contact Person: Prof. Dr. ... The ImageStreamX is a functional combination of a flow cytometer and a fluorescence microscope. ...
English: Flow cytometry is a method in cell biology that employs the deflection of laser light a well as the excitation of ... flow cytometry technique of suspending cells in a stream of fluid and passing them by an electronic detection apparatus ... Media in category "Flow cytometry". The following 200 files are in this category, out of 349 total. ... Pages in category "Flow cytometry". This category contains only the following page. ...
Introduction to Flow Cytometry and FACSDiva 6.0 tutorials.. *Tools for developing a flow cytometry experiment including a ... The training class will cover the basics of flow cytometry and an introduction to the use of the instrument and software ... The UMass Flow Core Facility has been a great resource for our research on brain development. Aside from the high quality ... Training on the flow analysis equipment for new students/postdocs and research technicians will be conducted quarterly, an ...
The most important clinical application of flow cytometry is in hematologic malignancy diagnosis. ... The development of a specialized flow cytometer in the 1970s, triggered by the HIV pandemic, paved the way to a broad range of ... Flow cytometry is an invaluable tool used to analyze the chemical and physical properties of cells. This laboratory technique ... Role of antibodies in flow cytometry. Antibodies are an invaluable component of flow cytometry. The advent of monoclonal ...
You must make an appointment to utilize the staff-assisted machines. Please plan your experiments ahead and make sure the facility can accommodate your schedule. We recommend a minimum of 2 week advanced bookings. Please keep your appointments, and show up for them promptly. You will be billed for late cancellations.. For appointments and consultations, contact Facility Manager Rochelle Diamond, x3998 (afternoons), x4947 (message), at [email protected] A completed Biosafety Form must be submitted prior to requesting an appointment. ...
CA Flow Cytometry Website Flow cytometry measures and analyzes the characteristics of single particles, normally cells, as they ... Tens of thousands of cells can be interrogated by a flow cytometer in a single second. Flow cytometry can be used for a variety ... Thousands of cells can be analyzed by a flow cytometer in a single second. Among the measurements derived from flow cytometry ... The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps ...
Flow Cytometry. La Jolla, California Campus. The Flow Cytometry Core Facility is home to a variety of fluorescence-based ... Introduction to Flow Cytometry Web-Based Training] - BD Biosciences. \n*[Multicolor Flow Cytometry Resources] - BD Biosciences ... Practical Flow Cytometry] - Howard M. Shapiro, 2003. \n" }, { "title": "Flow Cytometry Organizations", "content": "\n*[ISAC - ... Current Protocols in Cytometry] - Scripps Research Network or valid login only. \n*[Cytometry Protocols] - Purdue University ...
The introduction of smaller and easy-to-operate laser systems will further expand applications of flow cytometry to routine ... and suggests specific product and marketing opportunities for flow cytometry instruments and reagents.. Rationale. During the ... has announced the addition of the 2015 Strategies in the Global Flow Cytometry Market report to their offering. ... will have a profound impact on the flow cytometry markets worldwide. New molecular diagnostic and monoclonal antibody tests ...
Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test. ...
... sills at CALSHP.CALS.WISC.EDU sills at CALSHP.CALS.WISC.EDU Tue Oct 20 12:35:46 EST 1992 *Previous message: new ... I would appreciate hearing from anybody who has experience with flow cytometry in Arabidopsis. Short of that, I am interested ...
The standard reference for anyone interested in understanding flow cytometry technology.. American Journal of Clinical ... Practical Flow Cytometry. Copyright © 2003 John Wiley & Sons, Inc. All rights reserved. ...
Flow Cytometry support is not available on University or bank holidays.. Within normal working hours. Liaise with the ... Flow Cytometry Core Facility. Newcastle University , Flow Cytometry Core Facility , Services. Top Services. Services. Overview ...
... Becton Dickinson Immune Function Laboratory. Location: Becton Dickinson Immune ... Our experienced staff is pleased to offer flow cytometry training as well as assisted and independent instrument use. Please ... Examples of services offered include flow cytometry, cell sorting, cytokine/chemokine analysis, and experimental design. ... To learn more about the Ross facility, visit the Flow Cytometry website. ...
... flow cytometry is forcing cells to pass detectors in single file. But it is also looking into cells more deeply than ever, ... GEN: Flow cytometry continues to evolve from its origins as a research tool. Outside the laboratory, where is flow cytometry ... Garret Guenther, Ph.D., Global Support Manager, Flow Cytometry, ACEA Biosciences. GEN: How is flow cytometry contributing to ... These activities, we have determined, are being facilitated by flow cytometry.. At Bio-Rad, we have incorporated flow cytometry ...
Fluorescence microscopy and flow cytometry are used to quantify the total number and type of cells in a sample. They differ ... What is flow cytometry?. With flow cytometry, the properties of cells can be quantified. This is done on an individual basis ... Flow cytometry, however, can handle larger volumes of up to hundreds of thousands of cells. Furthermore, flow cytometry can use ... Fluorescence microscopy and flow cytometry in use. As mentioned, while fluorescence microscopy and flow cytometry achieve the ...
... Welcome to the Ross Flow Cytometry Core Facility. We are located on the 10th floor of the ... Home , Flow Cytometry Facility , Ross Flow Cytometry. ... The core offers state-of-the-art flow cytometry cell analysis ... Please do not hesitate to contact us if you have any questions about potential uses of flow cytometry for your research ... how to appropriately design experiments for flow cytometry, prepare samples, or how to analyze your data. ...
The Core Facility Flow Cytometry is also involved in training and education of PhD-students and members of the DKFZ (see ... Head of Core Facility Flow Cytometry (FACS ). E-Mail: [email protected] Tel.:. +49 6221 42-1261 (Office). +49 ... Flow Cytometry is a powerful analysis tool in modern biology and life sciences. The applications are numerous and have ... The core facility flow cytometry is equipped with various instruments and software modules to analyse and sort your samples. ...
... part 1 This forms the first session of the Garvan Flow , Flow Cytometry Training course. this is a 1 1/2 day training course a… ... BASICS OF FLOW CYTOMETRY ? Measurement METRY Cells CYTO * 14. BASICS OF FLOW CYTOMETRY ? Measurement METRY Cells CYTO Flow Flow ... Flow Cytometry Measurement METRY Cells CYTO Flow Flow * 16. FLOW CELL Hydrodynamic focusing of sample to laser intercept - ( ... Flow Cytometry Training talks - part 1 This forms the first session of the Garvan Flow , Flow Cytometry Training course. this ...
Newcastle University , Flow Cytometry Core Facility , Core Technologies , Mass Cytometry. Top Mass Cytometry. Mass Cytometry. ... The technology is conceptually akin to traditional fluorescent flow cytometry except that antibodies and detection reagents are ... The Helios is the latest generation of the Cytof Mass Cytometer combining a flow cytometer with the power of a Time of Flight ... These systems combine the sample delivery and data output of a flow cytometer with the detection capabilities of a Time of ...
Flow cytometry. BrisSynBio has access to the equipment and expertise housed in the Flow Cytometry Facility. This comprises high ... Flow cytometry Equipment booking BrisSynBio users are welcome to book our equipment. ... Flow cytometry provides a technique for obtaining information about cells or other particles in a suitable buffer based on ... The Flow Cytometry Facility is located in Cellular and Molecular Medicine, in the Biomedical Sciences Building. ...
  • The Flow and Image Cytometry Laboratory provides the University of Oklahoma research community with state-of-the-art cell analysis and sorting instrumentation, and the technical expertise to best utilize this technology. (i2e.org)
  • The Laboratory for Molecular Biology and Cytometry Research is a state of the art facility offeri. (i2e.org)
  • Antibodies are an invaluable component of flow cytometry. (news-medical.net)
  • The advent of monoclonal antibodies in 1977 promised an unlimited supply of highly specific antibodies and dramatically changed the flow cytometry technique. (news-medical.net)
  • The development of monoclonal antibodies against specific phosphoepitopes that can help in detection of protein activation states have enabled the use of flow cytometry to study cellular function. (news-medical.net)
  • Also, high concentrations of antibodies in flow cytometry can lead to non-specific binding which will complicate the process. (news-medical.net)
  • Therefore, these aggregates and polymers need to be removed from antibodies before using them in flow cytometry. (news-medical.net)
  • Sigma® Life Science, the Life Science division of Sigma-Aldrich, provides monoclonal antibodies validated specifically for flow cytometry in pure form or conjugated to some commonly used fluorochromes. (news-medical.net)
  • Thermo Fisher Scientific offers antibodies conjugated to 24 different fluorescent dyes and proteins for use in flow cytometry. (news-medical.net)
  • During the next five years, continued advances in molecular diagnostics, monoclonal antibodies, lasers and IT, as well as growing understanding of immunologic forces regulating systemic diseases, will have a profound impact on the flow cytometry markets worldwide. (prnewswire.com)
  • This article reviews technical aspects of flow cytometry, availability of antibodies suitable for studies in domestic animals, and applications in veterinary oncology with emphasis on characterization of round cell tumors. (mendeley.com)
  • Flow cytometry, utilizing antibodies recognizing targets restricted to the hPSC surfaceome, offers an invaluable tool for high-throughput validation of hPSC lines. (springer.com)
  • Here we describe the immunophenotyping of live human embryonic stem cell (hESC, H9) and human induced pluripotent stem cell (hiPSC, KB3) lines by flow cytometry using a panel of antibodies identified as either stem cell reference markers (CD90, EpCam) or reported as being prevalent or restricted (c-Kit, HPI-1, Integrin α6, Semaphorin-6A) to these cells. (springer.com)
  • Choose from hundreds of flow cytometry validated CST™ antibodies. (cellsignal.com)
  • Flow cytometric analysis, with monoclonal antibodies, has so far been restricted to leukocyte populations, which had been separated from erythrocytes before staining and/or analysis. (abcam.com)
  • We highly encourage you to contact the flow core for help with multi-color panel design before purchasing antibodies and for advice on sorting preparation. (slu.edu)
  • Thus combined with the detection of cell surface markers intracellular flow cytometry has evolved into a powerful platform, which enables characterization of signaling networks at a single cell level within phenotypically distinct cell populations. (cellsignal.com)
  • Intracellular flow cytometry offers unique advantages to the study of signaling events when compared to western blot analysis. (cellsignal.com)
  • Here, we provide a variety of protocols to aid in the preparation of cells for flow cytometry (how to do a proper antibody titration, general procedures to stain extracellular as well as intracellular antigens, etc. (uvm.edu)
  • Flow cytometry is a method in cell biology that employs the deflection of laser light a well as the excitation of fluorescent dyes to analyse various properties of a high number cells in a relatively short time. (wikimedia.org)
  • Lessened spill over compared to fluorescent cytometry. (ncl.ac.uk)
  • Not only can we use flow cytometry with associated fluorescent markers to understand the biology of cancer, but also the wide array of existing and developing markers provides us with important diagnostic tools in the detection of cancer early in either the malignant or relapse process. (springer.com)
  • Phagocytosis and postphagocytic reaction of cord blood and adult blood monocyte after infection with green fluorescent protein-labeled Escherichia coli and group B Streptococci," Cytometry B , vol. 76, no. 4, pp. 271-284, 2009. (hindawi.com)
  • Flow cytometry, typically using fluorescent probes that bind to specific cell-associated molecules, allows measurements of various phenotypic, biochemical and molecular characteristics of individual cells (or particles) suspended in a fluid stream. (slu.edu)
  • Flow Cytometry is a technique that allows for the rapid measurement of characteristics of small particles, such as cells, microspheres, or certain molecules, through the use of fluorescent markers and scattered light, and the isolation of particles with unique properties based on those markers. (yalecancercenter.org)
  • This kit contains suspensions of four high intensity microparticles, which have been tested under flow cytometry conditions for uniformity of size and fluorescent signal. (polysciences.com)
  • Factors such as continuous technological advancements in the field of flow cytometry, prevalence of target diseases (such as HIV/AIDS and cancer), growing adoption of flow cytometry in advanced research activities and clinical trials, and the increasing availability of novel application-specific flow cytometry products are driving the growth of the flow cytometry market. (marketsandmarkets.com)
  • The result is a flow cytometry readout with information about whether given proteins are present, and their numbers. (wisegeek.com)
  • If appropriate, a doctor may recommend platelet flow cytometry for this ongoing monitoring, in the interest of keeping a close eye on platelet counts and specific proteins in the blood. (wisegeek.com)
  • The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps on a fee-for-service basis. (scripps.edu)
  • this is a 1 1/2 day training course aimed at giving new and experienced researchers a better understanding of cytometry in medical and biological research. (slideshare.net)
  • The software developed by the researchers generates a two-parameter dot-plot display, similar to those found in flow cytometry, offering the objectivity of the latter method but with tools generally available to hematopathologists and other clinicians. (photonics.com)
  • Researchers have developed a method for converting immunohistochemistry data into objective, flow cytometrylike information. (photonics.com)
  • We provide a flow cytometry service to medical researchers in sydney australia. (freelancer.com)
  • Learn researchers how to analyze flow cytometry data in an efficient way using FlowJo v10,the world's nr.1 analysis software tool for flow cytometry data. (vib.be)
  • The core staff is here to help all researchers, whether novice or expert in flow cytometry, understand how this powerful technology can benefit your research endeavors. (slu.edu)
  • The services complement researchers' work in flow cytometry and allows for custom pipeline development, including plugin "app" development for the FlowJo application. (businesswire.com)
  • Flow cytometry is also finding new uses in familiar settings, such as the research laboratory and the biopharmaceutical production line. (genengnews.com)
  • Outside the laboratory, where is flow cytometry making significant contributions? (genengnews.com)
  • Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. (booktopia.com.au)
  • Dr Filby is also an International Society for the Advancement of Cytometry (ISAC) Shared Resource Laboratory Emerging Leader (SRL-EL) and is heavily involved in a number of educational initiatives for cytometry at both national and international levels. (rms.org.uk)
  • After studying Chemistry at Salford University, Richard worked in the oligonucleotide synthesis group at the MRC's Laboratory of Molecular Biology, before changing direction and running the flow cytometry equipment for the LMB for 8 years. (rms.org.uk)
  • Flow cytometry has found increasing application in the field of apoptosis research. (booktopia.com.au)
  • NIR Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) ab112149 is designed to detect cell apoptosis by measuring the loss of the mitochondrial membrane potential. (abcam.com)
  • More recently, flow cytometry has been used to improve the analysis of small particles such as circulating exosomes, which promise to inform liquid biopsies. (genengnews.com)
  • The point at which the particles are delivered into the sheath fluid is commonly termed the Flow Cell. (sheffield.ac.uk)
  • A flow cytometry apparatus for determining one or more characteristics of particles or the like flowing in a liquid stream includes a nozzle for generating a liquid flow stream for moving particles therethrough substantially one at a time. (google.ca)
  • Flow cytometry analyzes the whole blood component, i.e., physical and chemical characteristics of the particles present in the blood. (mynewsdesk.com)
  • All particles have been screened by flow cytometry labs to be suitable for instrument alignment. (polysciences.com)
  • Training on the flow analysis equipment for new students/postdocs and research technicians will be conducted quarterly, an email will be sent to the flow-core-l email list and the schedule will be posted. (umass.edu)
  • Chargeback costs are quite reasonable considering her expertise on the machine and I would highly recommend using this core to anyone needing flow cytometry. (umass.edu)
  • The core offers state-of-the-art flow cytometry cell analysis and high-speed sorting to the research community at Hopkins. (hopkinsmedicine.org)
  • The mission of URMC Flow Cytometry Core is to provide investigators with state-of-the-art instrumentation along with the human expertise to support all that is possible now, while pushing the limits of what can be done with flow cytometry. (rochester.edu)
  • The URMC Flow Core sincerely thanks the University of Rochester Clinical & Translational Science Institute's (Grant number UL1 RR024160) for their considerable and continued support of mission critical equipment. (rochester.edu)
  • The URMC Flow Cytometry Core sincerely thanks the UR Developmental Center for AIDS Research (P30AI078498, NIH/NIAID) for their support in the acquisition of the Amnis ImageStreamX. (rochester.edu)
  • The URMC Flow Core would also like to thank the Rochester Human Immunology Center for their support of mission critical equipment. (rochester.edu)
  • The URMC Flow Core is grateful to NCRR for the funding of a new Aria II high speed cell sorter in part from grant number 1S10RR0292299-01. (rochester.edu)
  • Comments about the Flow Core? (rochester.edu)
  • The core will have a configuration up that user will be able to access to better enable them to design multi-parametric panels that will work with the instruments found in the NSU Flow Core. (nova.edu)
  • You can have your files uploaded to an external memory device to take back to your own lab for data analysis or you may request that the flow core staff analyze the data for you. (slu.edu)
  • The UMMC Cancer Center and Research Institute Flow Cytometry Core provides state-of-the-art flow cytometry analysis and related services to the UMMC research community and affiliated institutions. (umc.edu)
  • The core provides flow cytometry analysis and sorting, as well as consultation for experimental design and data interpretation. (umc.edu)
  • He is currently head of the Flow Cytometry Core Facilit (FCCF) at Newcastle University leading a dedicated team of flow cytometry specialists with the sole aim of providing a comprehensive, cutting edge cytometry resource to the wider research community at Newcastle University and beyond. (rms.org.uk)
  • On the basis of product, the hematology and flow cytometry market is segmented into flow cytometry instruments, reagent & consumables, and accessories. (mynewsdesk.com)
  • Tools for developing a flow cytometry experiment including a spectrum viewer of commonly used fluorochromes and a multicolor panel designer. (umass.edu)
  • Flow cytometry is validating transfection, confirming whether desirable edits have been achieved, measuring the functional effects of gene editing, and enriching cell populations based on functional cell sorting. (genengnews.com)
  • The Flow Cytometry Lysing Solution can be used with or without sample washing and is suitable for the analysis of normal and malignant leukocyte populations derived from various human biological samples (blood, bone marrow and others) using Flow Cytometry. (abcam.com)
  • With Abcam's Flow Cytometry Lysing Solution the analysis of whole blood by flow cytometry has become as easy and accurate as the analysis of separated cell populations. (abcam.com)
  • Moreover, flow cytometric cell sorting techniques, up to the level of single cell sorting, permit further investigation and modulation of specific cell populations for scientific or clinical purposes under experimentally defined conditions. (rug.nl)
  • The quantitative values derived with flow cytometry can be both relative and absolute values, but are typically relative. (news-medical.net)
  • A new system for automated flow cytometry sample preparation, the Laminar Wash™ AUTO 1000, offers scientists a fully-automated flow cytometry staining platform designed to produce the most quantitative and reproducible results for flow cytometry. (genengnews.com)
  • Laserglow lasers are integrated into several OEM cytometry systems where our DPSS systems provide the light source in a stable, cost-effective manner. (photonics.com)
  • Be sure that your flow machine is equipped with the proper filters and lasers for the fluors you are interested in using. (biolegend.com)
  • Together with FlowJo ® , the leading single-cell flow cytometry analysis software, the partnership aims to put the most powerful bioinformatics approaches into the hands of anyone who needs them, in a manner that is familiar and friendly to use. (businesswire.com)
  • I knew over 10 years ago when we started developing automated analysis tools for flow cytometry we were never going to come close to being able to deliver the ease of use that users gain from FlowJo. (businesswire.com)
  • In the end you will be able to analyze and visualize your flow data and learn how to get the statistics out of it. (vib.be)
  • Guava® and Muse® flow cytometry kits are designed for the analysis of cellular events and/or cell phenotypes. (luminexcorp.com)
  • In its present configuration, the sorter is capable of performing 20 parameters (forward and side scatter plus eighteen color flow cytometry). (nova.edu)
  • In its present configuration, the analyzer is capable of performing twenty parameters (forward and side scatter plus eighteen color flow cytometry). (nova.edu)
  • While the combination of flow cytometry and single-cell technology is still new, it is already defining new developmental states, identifying unculturable microbes, and revealing how cellular heterogeneity relates to health and disease. (genengnews.com)
  • Flow cytometry promises to be a useful tool in this field, allowing the determination of different cellular, dissolved, and functional pathophysiological components of sepsis. (hindawi.com)
  • The size and shape of the antibody used and its conjugates influence the staining measurements in flow cytometry, especially in the case of cytoplasmic or intranuclear staining. (news-medical.net)
  • Antibody stability is another concern that affects staining in flow cytometry. (news-medical.net)
  • All these factors need to be carefully considered before the formulation of antibody-conjugates for flow cytometry. (news-medical.net)
  • Abcam's Flow Cytometry Lysing Solution is a ready-to-use solution formulated for lysing erythrocytes following monoclonal antibody staining of whole blood. (abcam.com)
  • Turn complexity into clarity by choosing the ideal flow cytometry platform for your lab, your budget, and your experimental design. (sigmaaldrich.com)
  • Over the years, updates were incorporated to adapt to technological advancements in both flow cytometry and computing technologies. (wikipedia.org)