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FlavivirusYellow fever virusEncephalitis Viruses, Tick-BorneYellow FeverEncephalitis, Tick-BorneFlavivirus InfectionsWest Nile virusEncephalitis, St. LouisEncephalitis Virus, St. LouisDengue VirusIndianaEncephalitis Virus, JapaneseEncephalitis, JapaneseAlphavirusLaboratoriesAlphavirus InfectionsClinical Laboratory TechniquesPublic HealthHydranencephalyClassical swine fever virusClassical Swine FeverBorder disease virusDiarrhea Viruses, Bovine ViralBorder DiseasePestivirusJob SatisfactionVentilation-Perfusion RatioJob DescriptionLungJob ApplicationOccupational DiseasesEmploymentDengueSevere DengueNational Institute of Allergy and Infectious Diseases (U.S.)Hemorrhagic Fevers, ViralChikungunya virusWest Nile Fever
Flavivirus: A genus of FLAVIVIRIDAE containing several subgroups and many species. Most are arboviruses transmitted by mosquitoes or ticks. The type species is YELLOW FEVER VIRUS.Yellow fever virus: The type species of the FLAVIVIRUS genus. Principal vector transmission to humans is by AEDES spp. mosquitoes.Encephalitis Viruses, Tick-Borne: A subgroup of the genus FLAVIVIRUS that causes encephalitis and hemorrhagic fevers and is found in eastern and western Europe and the former Soviet Union. It is transmitted by TICKS and there is an associated milk-borne transmission from viremic cattle, goats, and sheep.Yellow Fever: An acute infectious disease primarily of the tropics, caused by a virus and transmitted to man by mosquitoes of the genera Aedes and Haemagogus. The severe form is characterized by fever, HEMOLYTIC JAUNDICE, and renal damage.Encephalitis, Tick-Borne: Encephalitis caused by neurotropic viruses that are transmitted via the bite of TICKS. In Europe, the diseases are caused by ENCEPHALITIS VIRUSES, TICK-BORNE, which give rise to Russian spring-summer encephalitis, central European encephalitis, louping ill encephalitis, and related disorders. Powassan encephalitis occurs in North America and Russia and is caused by the Powassan virus. ASEPTIC MENINGITIS and rarely encephalitis may complicate COLORADO TICK FEVER which is endemic to mountainous regions of the western United States. (From Joynt, Clinical Neurology, 1996, Ch26, pp14-5)Flavivirus Infections: Infections with viruses of the genus FLAVIVIRUS, family FLAVIVIRIDAE.West Nile virus: A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE). It can infect birds and mammals. In humans, it is seen most frequently in Africa, Asia, and Europe presenting as a silent infection or undifferentiated fever (WEST NILE FEVER). The virus appeared in North America for the first time in 1999. It is transmitted mainly by CULEX spp mosquitoes which feed primarily on birds, but it can also be carried by the Asian Tiger mosquito, AEDES albopictus, which feeds mainly on mammals.Encephalitis, St. Louis: A viral encephalitis caused by the St. Louis encephalitis virus (ENCEPHALITIS VIRUS, ST. LOUIS), a FLAVIVIRUS. It is transmitted to humans and other vertebrates primarily by mosquitoes of the genus CULEX. The primary animal vectors are wild birds and the disorder is endemic to the midwestern and southeastern United States. Infections may be limited to an influenza-like illness or present as an ASEPTIC MENINGITIS or ENCEPHALITIS. Clinical manifestations of the encephalitic presentation may include SEIZURES, lethargy, MYOCLONUS, focal neurologic signs, COMA, and DEATH. (From Adams et al., Principles of Neurology, 6th ed, p750)Encephalitis Virus, St. Louis: A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE), which is the etiologic agent of ST. LOUIS ENCEPHALITIS in the United States, the Caribbean, and Central and South America.Dengue Virus: A species of the genus FLAVIVIRUS which causes an acute febrile and sometimes hemorrhagic disease in man. Dengue is mosquito-borne and four serotypes are known.IndianaEncephalitis Virus, Japanese: A species of FLAVIVIRUS, one of the Japanese encephalitis virus group (ENCEPHALITIS VIRUSES, JAPANESE), which is the etiological agent of Japanese encephalitis found in Asia, southeast Asia, and the Indian subcontinent.Encephalitis, Japanese: A mosquito-borne encephalitis caused by the Japanese B encephalitis virus (ENCEPHALITIS VIRUS, JAPANESE) occurring throughout Eastern Asia and Australia. The majority of infections occur in children and are subclinical or have features limited to transient fever and gastrointestinal symptoms. Inflammation of the brain, spinal cord, and meninges may occur and lead to transient or permanent neurologic deficits (including a POLIOMYELITIS-like presentation); SEIZURES; COMA; and death. (From Adams et al., Principles of Neurology, 6th ed, p751; Lancet 1998 Apr 11;351(9109):1094-7)Alphavirus: A genus of TOGAVIRIDAE, also known as Group A arboviruses, serologically related to each other but not to other Togaviridae. The viruses are transmitted by mosquitoes. The type species is the SINDBIS VIRUS.Laboratories: Facilities equipped to carry out investigative procedures.Alphavirus Infections: Virus diseases caused by members of the ALPHAVIRUS genus of the family TOGAVIRIDAE.Clinical Laboratory Techniques: Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease.Public Health: Branch of medicine concerned with the prevention and control of disease and disability, and the promotion of physical and mental health of the population on the international, national, state, or municipal level.Hydranencephaly: A congenital condition where the greater portions of the cerebral hemispheres and CORPUS STRIATUM are replaced by CSF and glial tissue. The meninges and the skull are well formed, which is consistent with earlier normal embryogenesis of the telencephalon. Bilateral occlusions of the internal carotid arteries in utero is a potential mechanism. Clinical features include intact brainstem reflexes without evidence of higher cortical activity. (Menkes, Textbook of Child Neurology, 5th ed, p307)Classical swine fever virus: A species of the PESTIVIRUS genus causing exceedingly contagious and fatal hemorrhagic disease of swine.Classical Swine Fever: An acute, highly contagious disease affecting swine of all ages and caused by the CLASSICAL SWINE FEVER VIRUS. It has a sudden onset with high morbidity and mortality.Border disease virus: A species of PESTIVIRUS causing a congenital sheep disease characterized by an abnormally hairy birth-coat, tremors, and poor growth.Diarrhea Viruses, Bovine Viral: A group of viruses in the genus PESTIVIRUS, causing diarrhea, fever, oral ulcerations, hemorrhagic syndrome, and various necrotic lesions among cattle and other domestic animals. The two species (genotypes), BVDV-1 and BVDV-2 , exhibit antigenic and pathological differences. The historical designation, BVDV, consisted of both (then unrecognized) genotypes.Border Disease: Congenital disorder of lambs caused by a virus closely related to or identical with certain strains of bovine viral diarrhea virus.Pestivirus: A genus of FLAVIVIRIDAE, also known as mucosal disease virus group, which is not arthropod-borne. Transmission is by direct and indirect contact, and by transplacental and congenital transmission. Species include BORDER DISEASE VIRUS, bovine viral diarrhea virus (DIARRHEA VIRUS, BOVINE VIRAL), and CLASSICAL SWINE FEVER VIRUS.Job Satisfaction: Personal satisfaction relative to the work situation.Ventilation-Perfusion Ratio: The ratio of alveolar ventilation to simultaneous alveolar capillary blood flow in any part of the lung. (Stedman, 25th ed)Job Description: Statement of the position requirements, qualifications for the position, wage range, and any special conditions expected of the employee.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Job Application: Process of applying for employment. It includes written application for employment or personal appearance.Occupational Diseases: Diseases caused by factors involved in one's employment.Employment: The state of being engaged in an activity or service for wages or salary.Dengue: An acute febrile disease transmitted by the bite of AEDES mosquitoes infected with DENGUE VIRUS. It is self-limiting and characterized by fever, myalgia, headache, and rash. SEVERE DENGUE is a more virulent form of dengue.Severe Dengue: A virulent form of dengue characterized by THROMBOCYTOPENIA and an increase in vascular permeability (grades I and II) and distinguished by a positive pain test (e.g., TOURNIQUET PAIN TEST). When accompanied by SHOCK (grades III and IV), it is called dengue shock syndrome.National Institute of Allergy and Infectious Diseases (U.S.): Component of the NATIONAL INSTITUTES OF HEALTH. It conducts and supports basic and applied research to better understand, treat, and ultimately prevent infectious, immunologic, and allergic diseases. It was established in 1948.Hemorrhagic Fevers, Viral: A group of viral diseases of diverse etiology but having many similar clinical characteristics; increased capillary permeability, leukopenia, and thrombocytopenia are common to all. Hemorrhagic fevers are characterized by sudden onset, fever, headache, generalized myalgia, backache, conjunctivitis, and severe prostration, followed by various hemorrhagic symptoms. Hemorrhagic fever with kidney involvement is HEMORRHAGIC FEVER WITH RENAL SYNDROME.Chikungunya virus: A species of ALPHAVIRUS causing an acute dengue-like fever.West Nile Fever: A mosquito-borne viral illness caused by the WEST NILE VIRUS, a FLAVIVIRUS and endemic to regions of Africa, Asia, and Europe. Common clinical features include HEADACHE; FEVER; maculopapular rash; gastrointestinal symptoms; and lymphadenopathy. MENINGITIS; ENCEPHALITIS; and MYELITIS may also occur. The disease may occasionally be fatal or leave survivors with residual neurologic deficits. (From Joynt, Clinical Neurology, 1996, Ch26, p13; Lancet 1998 Sep 5;352(9130):767-71)

Noncytopathic flavivirus replicon RNA-based system for expression and delivery of heterologous genes. (1/427)

Noncytopathic replicons of the flavivirus Kunjin (KUN) were employed for expression and delivery of heterologous genes. Replicon vector C20DX2Arep, containing a unique cloning site followed by the sequence of 2A autoprotease of foot-and-mouth disease virus, was constructed and used for expression of a number of heterologous genes including chloramphenicol acetyltransferase (CAT), green fluorescent protein (GFP), beta-galactosidase, glycoprotein G of vesicular stomatitis virus, and the Core and NS3 genes of hepatitis C virus. The expression and proper processing of these genes upon transfection of BHK21 cells with the recombinant replicon RNAs were demonstrated by immunofluorescence, radioimmunoprecipitation, and appropriate reporter gene assays. Most of these recombinant KUN replicon RNAs were also successfully packaged into secreted virus-like particles (VLPs) by subsequent transfection with Semliki Forest virus replicon RNA expressing KUN structural genes. Infection of BHK21 and Vero cells with these VLPs resulted in continuous replication of the recombinant replicon RNAs and prolonged expression of the cloned genes without any cytopathic effect. We also developed a replicon vector for generation of stable cell lines continuously expressing heterologous genes by inserting an encephalomyelocarditis virus internal ribosomal entry site-neomycin transferase gene cassette into the 3'-untranslated region of the C20DX2Arep vector. Using this vector (C20DX2ArepNeo), stable BHK cell lines persistently expressing GFP and CAT genes for up to 17 passages were established. Thus noncytopathic KUN replicon vectors with the ability to be packaged into VLPs should provide a useful tool for the development of noninfectious and noncytopathic vaccines as well as for gene therapy applications.  (+info)

Mutation patterns for two flaviviruses: hepatitis C virus and GB virus C/hepatitis G virus. (2/427)

We studied the mutation patterns of hepatitis C virus (HCV) and GB virus C/hepatitis G virus (HGV). Although the mutation patterns of the two viruses were similar to each other, they were quite different from that of HIV. In particular, the similarity of the patterns between HCV or HGV and human nuclear pseudogenes was statistically significant whereas there was no similarity between HIV and human nuclear pseudogenes. This finding suggests that the mutation patterns of HCV and HGV are similar to the patterns of spontaneous substitution mutations of human genes, implying that nucleotide analogues which are effective against HCV and HGV may have a side effect on the normal cells of humans.  (+info)

Degradation of Japanese encephalitis virus by neutrophils. (3/427)

The ability of neutrophils to degrade the phagocytosed Japanese encephalitis (JE) virion, via triggering of the respiratory burst and generation of toxic radicals has been investigated. JEV or JEV-induced macrophage derived factor (MDF) induces increase in intracellular oxidative signals with generation of superoxide anion (O2-), via activation of cytosolic NADPH and subsequent formation of hydrogen peroxide, with maximum activity on day 7 post infection. The response was sensitive to anti-MDF antibody treatment. Further, the study revealed rapid degradation of phagocytosed JE viral protein and nucleic acid. The viral protein degradation was partially dependent on the generation of toxic oxygen species as it could be abrogated by pretreatment of the cells with staurosporine.  (+info)

Mutagenesis of the NS2B-NS3-mediated cleavage site in the flavivirus capsid protein demonstrates a requirement for coordinated processing. (4/427)

Analysis of flavivirus polyprotein processing has revealed the presence of a substrate for the virus-encoded NS2B-NS3 protease at the carboxy-terminal end of the C (capsid or core) protein. Cleavage at this site has been implicated in the efficient generation of the amino terminus of prM via signal peptidase cleavage. Yellow fever virus has four basic residues (Arg-Lys-Arg-Arg) in the P1 through P4 positions of this cleavage site. Multiple alanine substitutions were made for these residues in order to investigate the substrate specificity and biological significance of this cleavage. Mutants were analyzed by several methods: (i) a cell-free trans processing assay for direct analysis of NS2B-NS3-mediated cleavage; (ii) a trans processing assay in BHK-21 cells, using a C-prM polyprotein, for analysis of prM production; (iii) an infectivity assay of full-length transcripts to determine plaque-forming ability; and (iv) analysis of proteins expressed from full-length transcripts to assess processing in the context of the complete genome. Mutants that exhibited severe defects in processing in vitro and in vivo were incapable of forming plaques. Mutants that contained two adjacent basic residues within the P1 through P4 region were processed more efficiently in vitro and in vivo, and transcripts bearing these mutations were fully infectious. Furthermore, two naturally occurring plaque-forming revertants were analyzed and shown to have restored protein processing phenotypes in vivo. Finally, the efficient production of prM was shown to be dependent on the proteolytic activity of NS3. These data support a model of two coordinated cleavages, one that generates the carboxy terminus of C and another that generates the amino terminus of prM. A block in the viral protease-mediated cleavage inhibits the production of prM by the signal peptidase, inhibits particle release, and eliminates plaque formation.  (+info)

Transient expression of cellular polypyrimidine-tract binding protein stimulates cap-independent translation directed by both picornaviral and flaviviral internal ribosome entry sites In vivo. (5/427)

The regulation of cap-independent translation directed by the internal ribosome entry sites (IRESs) present in some viral and cellular RNAs is poorly understood. Polypyrimidine-tract binding protein (PTB) binds specifically to several viral IRESs. IRES-directed translation may be reduced in cell-free systems that are depleted of PTB and restored by reconstitution of lysates with recombinant PTB. However, there are no data concerning the effects of PTB on IRES-directed translation in vivo. We transfected cells with plasmids expressing dicistronic transcripts in which the upstream cistron encoded PTB or PTB deletion mutants (including a null mutant lacking amino acid residues 87 to 531). The downstream cistron encoded a reporter protein (chloramphenicol acetyltransferase [CAT]) under translational control of the poliovirus IRES which was placed within the intercistronic space. In transfected BS-C-1 cells, transcripts expressing wild-type PTB produced 12-fold more reporter protein than similar transcripts encoding the PTB null mutant. There was a 2.4-fold difference in CAT produced from these transcripts in HeLa cells, which contain a greater natural abundance of PTB. PTB similarly stimulated CAT production from transcripts containing the IRES of hepatitis A virus or hepatitis C virus in BS-C-1 cells and Huh-7 cells (37- to 44-fold increase and 5 to 5.3-fold increase, respectively). Since PTB had no quantitative or qualitative effect on transcription from these plasmids, we conclude that PTB stimulates translation of representative picornaviral and flaviviral RNAs in vivo. This is likely to reflect the stabilization of higher ordered RNA structures within the IRES and was not observed with PTB mutants lacking RNA recognition motifs located in the C-terminal third of the molecule.  (+info)

Phylogeny of the genus flavivirus using complete coding sequences of arthropod-borne viruses and viruses with no known vector. (6/427)

Attempts to define the evolutionary relationships and origins of viruses in the genus Flavivirus are hampered by the lack of genetic information particularly amongst the non-vectored flaviviruses. Using a novel protocol for sequence determination, the first complete coding sequence of St Louis encephalitis virus and those of two representative non-vectored flaviviruses, Rio Bravo (isolated from bat) and Apoi (isolated from rodent), are reported. The encoded polyproteins of Rio Bravo and Apoi virus are the smallest described to date within the genus FLAVIVIRUS: The highest similarities with other flaviviruses were found in the NS3 and NS5 genes. The proteolytic cleavage sites for the viral serine protease were highly conserved among the flaviviruses completely sequenced to date. Comparative genetic amino acid alignments revealed that p-distance cut-off values of 0.330-0.470 distinguished the arthropod-borne viruses according to their recognized serogroups and Rio Bravo and Apoi virus were assigned to two distinct non-vectored virus groups. Within these serogroups, cladogenesis based on the complete ORF sequence was similar to trees based on envelope and NS5 sequences. In contrast, branching patterns at the deeper nodes of the tree were different from those reported in the previous study of NS5 sequences. The significance of these observations is discussed.  (+info)

cis- and trans-acting elements in flavivirus RNA replication. (7/427)

Most of the seven flavivirus nonstructural proteins (NS1 to NS5) encoded in the distal two-thirds of the RNA positive-sense genome are believed to be essential components of RNA replication complexes. To explore the functional relationships of these components in RNA replication, we used trans-complementation analysis of full-length infectious RNAs of Kunjin (KUN) virus with a range of lethal in-frame deletions in the nonstructural coding region, using as helper a repBHK cell line stably producing functional replication complexes from KUN replicon RNA. Recently we showed that replication of KUN RNAs with large carboxy-terminal deletions including the entire RNA polymerase region in the NS5 gene, representing 34 to 75% of the NS5 coding content, could be complemented after transfection into repBHK cells. In this study we have demonstrated that KUN RNAs with deletions of 84 to 97% of the NS1 gene, or of 13 to 63% of the NS3 gene including the entire helicase region, were also complemented in repBHK cells with variable efficiencies. In contrast, KUN RNAs with deletions in any of the other four nonstructural genes NS2A, NS2B, NS4A, and NS4B were not complemented. We have also demonstrated successful trans complementation of KUN RNAs containing either combined double deletions in the NS1 and NS5 genes or triple deletions in the NS1, NS3, and NS5 genes comprising as much as 38% of the entire nonstructural coding content. Based on these and our previous complementation results, we have generated a map of cis- and trans-acting elements in RNA replication for the nonstructural coding region of the flavivirus genome. These results are discussed in the context of our model on formation and composition of the flavivirus replication complex, and we suggest molecular mechanisms by which functions of some defective components of the replication complex can be complemented by their wild-type counterparts expressed from another (helper) RNA molecule.  (+info)

A novel model for the study of the therapy of flavivirus infections using the Modoc virus. (8/427)

The murine Flavivirus Modoc replicates well in Vero cells and appears to be as equally sensitive as both yellow fever and dengue fever virus to a selection of antiviral agents. Infection of SCID mice, by either the intracerebral, intraperitoneal, or intranasal route, results in 100% mortality. Immunocompetent mice and hamsters proved to be susceptible to the virus only when inoculated via the intranasal or intracerebral route. Animals ultimately die of (histologically proven) encephalitis with features similar to Flavivirus encephalitis in man. Viral RNA was detected in the brain, spleen, and salivary glands of infected SCID mice and the brain, lung, kidney, and salivary glands of infected hamsters. In SCID mice, the interferon inducer poly IC protected against Modoc virus-induced morbidity and mortality and this protection was associated with a reduction in infectious virus content and viral RNA load. Infected hamsters shed the virus in the urine. This allows daily monitoring of (inhibition of) viral replication, by means of a noninvasive method and in the same animal. The Modoc virus model appears attractive for the study of chemoprophylactic or chemotherapeutic strategies against Flavivirus infections.  (+info)

The 3′ untranslated regions of Kamiti River virus and Cell fusing agent virus originated by self-duplication | Microbiology...The 3′ untranslated regions of Kamiti River virus and Cell fusing agent virus originated by self-duplication | Microbiology...
Previously, it was shown that the 3′ untranslated region (3′UTR) of Kamiti River virus (KRV) is nearly twice as long as the 3′UTR of other flaviviruses (1208 nucleotides compared with 730 nucleotides for the longest 3′UTR of any virus in the Tick-borne encephalitis virus species). Additionally, KRV and the closely related Cell fusing agent virus (CFAV) were shown to contain two short, almost perfect repeat sequences of 67 nucleotides. However, the construction of a robust comparative nucleotide alignment has now revealed that the double-length 3′UTR and the direct repeats resulted from the virtually complete duplication of a primordial KRV 3′UTR. We also propose that the CFAV 3′UTR was derived from a KRV-like precursor sequence with a large deletion that nevertheless preserved the two direct repeat sequences. These data provide new insights into the evolution of the flavivirus 3′UTR.
Purification and crystallization of Kokobera virus helicase - NERC Open Research ArchivePurification and crystallization of Kokobera virus helicase - NERC Open Research Archive
Kokobera virus is a mosquito-borne flavivirus belonging, like West Nile virus, to the Japanese encephalitis virus serocomplex. The flavivirus genus is characterized by a positive-sense single-stranded RNA genome. The unique open reading frame of the viral RNA is transcribed and translated as a single polyprotein which is post-translationally cleaved to yield three structural and seven nonstructural proteins, one of which is the NS3 gene that encodes a C-terminal helicase domain consisting of 431 amino acids. Helicase inhibitors are potential antiviral drugs as the helicase is essential to viral replication. Crystals of the Kokobera virus helicase domain were obtained by the hanging-drop vapour-diffusion method. The crystals belong to space group P3121 (or P3221), with unit-cell parameters a = 88.6, c = 138.6 Å, and exhibit a diffraction limit of 2.3 Å.. ...
IJERPH | Free Full-Text | Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and...IJERPH | Free Full-Text | Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and...
In Europe, many flaviviruses are endemic (West Nile, Usutu, tick-borne encephalitis viruses) or occasionally imported (dengue, yellow fever viruses). Due to the temporal and geographical co-circulation of flaviviruses in Europe, flavivirus differentiation by diagnostic tests is crucial in the adaptation of surveillance and control efforts. Serological diagnosis of flavivirus infections is complicated by the antigenic similarities among the Flavivirus genus. Indeed, most flavivirus antibodies are directed against the highly immunogenic envelope protein, which contains both flavivirus cross-reactive and virus-specific epitopes. Serological assay results should thus be interpreted with care and confirmed by comparative neutralization tests using a panel of viruses known to circulate in Europe. However, antibody cross-reactivity could be advantageous in efforts to control emerging flaviviruses because it ensures partial cross-protection. In contrast, it might also facilitate subsequent diseases, through a
Flavivirus - WikipediaFlavivirus - Wikipedia
Flavivirus is a genus of viruses in the family Flaviviridae. This genus includes the West Nile virus, dengue virus, tick-borne encephalitis virus, yellow fever virus, Zika virus and several other viruses which may cause encephalitis, as well as insect-specific flaviviruses (ISFs) such as cell fusing agent virus (CFAV), Palm Creek virus (PCV), and Parramatta River virus (PaRV). Flaviviruses are named from the yellow fever virus, the type virus for the family; the word flavus means "yellow" in Latin. The name yellow fever in turn originated from its propensity to cause yellow jaundice in victims. Flaviviruses share several common aspects: common size (40-65 nm), symmetry (enveloped, icosahedral nucleocapsid), nucleic acid (positive-sense, single-stranded RNA around 10,000-11,000 bases), and appearance in the electron microscope. Most of these viruses are transmitted by the bite from an infected arthropod (mosquito or tick) and hence, classified as arboviruses. Human infections with most of these ...
OPUS 4 | The role of integrins in flavivirus infectionOPUS 4 | The role of integrins in flavivirus infection
The Flavivirus genus (Flaviviridae family) comprises the most important arboviruses in the world such as dengue virus, West Nile virus (WNV), Zika virus (ZIKV), Japanese encephalitis virus and yellow fever virus (YFV). Every year, several outbreaks caused by flaviviruses are reported worldwide (i.e.: ZIKV and YFV outbreaks in South America) with a huge impact on economy and public health. In the last few decades, many aspects of the flavivirus biology and the interaction of flaviviruses with host cells have been elucidated. However, many underlying mechanisms concerning receptor usage, entry process and viral interaction with host cell factors are still not completely understood. Integrins, the major class of cell adhesion molecules have been implicated in the infectious cycle of different viruses including flaviviruses. A previous report proposed that a particular integrin, the αVβ3 integrin, might act as a cellular receptor for WNV. However, this hypothesis was not confirmed by other groups. In the
Japanese Encephalitis and West Nile and Other Flavivirus Infections<...Japanese Encephalitis and West Nile and Other Flavivirus Infections<...
TY - CHAP. T1 - Japanese Encephalitis and West Nile and Other Flavivirus Infections. AU - Watts, Douglas M.. AU - Granwehr, Bruno P.. AU - Shope, Robert E.. AU - Solomon, Tom. AU - Tesh, Robert B.. PY - 2006. Y1 - 2006. UR - http://www.scopus.com/inward/record.url?scp=36448954021&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=36448954021&partnerID=8YFLogxK. U2 - 10.1016/B978-0-443-06668-9.50078-8. DO - 10.1016/B978-0-443-06668-9.50078-8. M3 - Chapter. AN - SCOPUS:36448954021. SN - 9780443066689. VL - 1. SP - 823. EP - 830. BT - Tropical Infectious Diseases. PB - Elsevier Inc.. ER - ...
Mutational Evidence for an Internal Fusion Peptide in Flavivirus Envelope Protein E | Journal of VirologyMutational Evidence for an Internal Fusion Peptide in Flavivirus Envelope Protein E | Journal of Virology
The data presented here provide functional evidence that a highly conserved loop at the tip of each subunit of the flavivirus E protein is important for fusion activity and may be directly involved in interactions with target membranes during the initial stages of membrane fusion. The notion that this portion of the protein serves as an internal fusion peptide is consistent with earlier observations that antipeptide antibodies recognizing the region from amino acids 98 to 110 are capable of blocking low-pH-induced fusion of TBE virus with artificial membranes (47) and react more strongly with the low-pH form of the dengue 2 virus E protein than with the native neutral form (40). We have recently observed that the flavivirus cross-reactive MAb A1, whose binding was shown in the present study to be abolished by amino acid substitutions at Leu 107, is also capable of inhibiting virus-liposome fusion and binding of isolated E proteins to artificial membranes (K. Stiasny et al., unpublished data). ...
Development of a Rapid Solid Phase Immunoassay for the Detection of Flavivirus Antigens in Human Blood and Mosquito Vectors.Development of a Rapid Solid Phase Immunoassay for the Detection of Flavivirus Antigens in Human Blood and Mosquito Vectors.
Antigen-capture solid phase immunoassays employing flavivirus monoclonal antibodies MAB for the detection of yellow fever YF or dengue DEN viruses in cell cultures and serum have recently been reported. The assays, which employs virus type-specific, complex-reactive or group-reactive MAB in the capture phase, are reasonably sensitive and specific. The goal of our work has been the development of a generic flavivirus detection system and emphasis has been placed on an enzyme-linked immunoassay ELISA for the detection of DEN-infected mosquitoes. Attention has been directed at the identification and characterization of MAB with broad flavivirus group-reactive domain as determined by competitive binding assay with the expectation that such antibody combination will provide the best opportunity for antigen binding and therefore maximum assay sensitivity. However, since the available broadly group-reactive MAB has been found to exhibit at least some degree of cross-competition, flavivirus type-specific
Upregulation of signalase processing and induction of prM-E secretion by the flavivirus NS2B-NS3 protease: roles of protease...Upregulation of signalase processing and induction of prM-E secretion by the flavivirus NS2B-NS3 protease: roles of protease...
Recently, we have shown that the ability of the flavivirus NS2B-NS3 protease complex to promote efficient signalase processing of the C-prM precursor, as well as secretion of prM and E, does not appear to depend strictly on cleavage of the precursor at its Lys-Arg-Gly dibasic site by the protease. We suggested that the association of the protease with the precursor via NS2B may be sufficient by itself for the above effects. To study the proposed association in more detail, we have developed an assay in which processing at the C-prM dibasic cleavage site is abolished by Lys--,Gly conversion. We constructed deletion mutants and chimeras of the West Nile (WN) flavivirus NS2B protein and expressed them in the context of [5-C--,NS3(243)] containing either wild-type C-prM or its cleavage site mutant. All NS2B variants were able to form active protease complexes. Deletion of the carboxy-terminal cluster of hydrophobic amino acids in NS2B had no apparent effect on the formation of prM and prM-E ...
Uncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen | Research - Institut PasteurUncovering Flavivirus Host Dependency Factors through a Genome-Wide Gain-of-Function Screen | Research - Institut Pasteur
Link to Pubmed [PMID] - 30650657. Viruses 2019 01;11(1). Flaviviruses, such as dengue (DENV), West Nile (WNV), yellow fever (YFV) and Zika (ZIKV) viruses, are mosquito-borne pathogens that present a major risk to global public health. To identify host factors that promote flavivirus replication, we performed a genome-wide gain-of-function cDNA screen for human genes that enhance the replication of flavivirus reporter particles in human cells. The screen recovered seventeen potential host proteins that promote viral replication, including the previously known dolichyl-diphosphooligosaccharide-protein glycosyltransferase non-catalytic subunit (DDOST). Using silencing approaches, we validated the role of four candidates in YFV and WNV replication: ribosomal protein L19 (RPL19), ribosomal protein S3 (RPS3), DDOST and importin 9 (IPO9). Applying a panel of virological, biochemical and microscopic methods, we validated further the role of RPL19 and DDOST as host factors required for optimal ...
Flavivirus NS1: a multifaceted enigmatic viral protein | Virology Journal | Full TextFlavivirus NS1: a multifaceted enigmatic viral protein | Virology Journal | Full Text
Flaviviruses are emerging arthropod-borne viruses representing an immense global health problem. The prominent viruses of this group include dengue virus, yellow fever virus, Japanese encephalitis virus, West Nile virus tick borne encephalitis virus and Zika Virus. These are endemic in many parts of the world. They are responsible for the illness ranging from mild flu like symptoms to severe hemorrhagic, neurologic and cognitive manifestations leading to death. NS1 is a highly conserved non-structural protein among flaviviruses, which exist in diverse forms. The intracellular dimer form of NS1 plays role in genome replication, whereas, the secreted hexamer plays role in immune evasion. The secreted NS1 has been identified as a potential diagnostic marker for early detection of the infections caused by flaviviruses. In addition to the diagnostic marker, the importance of NS1 has been reported in the development of therapeutics. NS1 based subunit vaccines are at various stages of development. The
Zika virus structural biology and progress in vaccine development. | Biotechnol Adv;2017 Sep 12. | MEDLINE | VHL Regional...Zika virus structural biology and progress in vaccine development. | Biotechnol Adv;2017 Sep 12. | MEDLINE | VHL Regional...
The growing number of zika virus (ZIKV) infections plus a 20-fold increase in neonatal microcephaly in newborns in Brazil have raised alarms in many countries regarding the threat to pregnant women. Instances of microcephaly and central nervous system malformations continue to increase in ZIKV outbreak regions. ZIKV is a small enveloped positive-strand RNA virus belonging to the Flavivirus genus of the Flaviviridae family. High-resolution ZIKV structures recently identified by cryo-electron microscopy indicate that the overall ZIKV structure is similar to those of other flaviviruses. With its compact surface, ZIKV is more thermally stable than the dengue virus (DENV). ZIKV E proteins have a characteristic "herringbone" structure with a single glycosylation site. The ZIKV E protein, the major protein involved in receptor binding and fusion, is formed as a head-to-tail dimer on the surfaces of viral particles. The E monomer consists of three distinct domains: DI, DII, and DIII. The finger-like DII ...
An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine...An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine...
Abstract. Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak, serologic evidence of ROCV circulation has been reported and public health authorities are concerned about a return of ROCV outbreaks in Brazil. We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue samples were collected from infected animals for immunohistochemistry and molecular biology analysis. We observed the virus in the central nervous system, the inflammatory changes induced by Th1 and Th2 cytokines, and the final irreversible damage of nervous
View source for Zika virus - microbewikiView source for Zika virus - microbewiki
Baltimore Classification and Taxonomy== Group IV: (+) sense single-stranded RNA virus ,br>,br> , Order = [[Unassigned]] , Family = [[Flaviviridae]] , Genus = [[Flavivirus]] , species = [[Zika virus]],br> {,NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=64320&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy] Genome: ,font size="2">[http://www.ncbi.nlm.nih.gov/genome/6441 Genome],/font>,} ==Description and Significance== ,br> Zika virus (ZIKV) is a mosquito-borne flavivirus that was first isolated from a rhesus monkey in the Zika forest of Uganda in 1947. [[#Reference,[3]]] In 1968, isolation from human hosts occurred in residents of Nigeria. [[#Reference,[2]]] Since then, multiple studies have confirmed ZIKV antibody in humans from a multitude of countries in Africa and parts of Asia. [[#Reference,[2]]] In 2015, ZIKV first appeared outside of Africa and Asia when it was isolated in Brazil where is has caused a minor outbreak following the 2014 FIFA World Cup. ...
View source for Zika virus - microbewikiView source for Zika virus - microbewiki
Baltimore Classification and Taxonomy== Group IV: (+) sense single-stranded RNA virus ,br>,br> , Order = [[Unassigned]] , Family = [[Flaviviridae]] , Genus = [[Flavivirus]] , species = [[Zika virus]],br> {,NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&id=64320&lvl=3&lin=f&keep=1&srchmode=1&unlock Taxonomy] Genome: ,font size="2">[http://www.ncbi.nlm.nih.gov/genome/6441 Genome],/font>,} ==Description and Significance== ,br> Zika virus (ZIKV) is a mosquito-borne flavivirus that was first isolated from a rhesus monkey in the Zika forest of Uganda in 1947. [[#Reference,[3]]] In 1968, isolation from human hosts occurred in residents of Nigeria. [[#Reference,[2]]] Since then, multiple studies have confirmed ZIKV antibody in humans from a multitude of countries in Africa and parts of Asia. [[#Reference,[2]]] In 2015, ZIKV first appeared outside of Africa and Asia when it was isolated in Brazil where is has caused a minor outbreak following the 2014 FIFA World Cup. ...
Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil...Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil...
Molecular characterization of two rocio flavivirus strains isolated during the encephalitis epidemic in são paulo state, brazil and the development of a one-step rt-pcr assay for diagnosis; Caracterização molecular de duas cepas do flavivírus Rocio, isoladas durante a epidemia de encefalite no Estado de São Paulo, Brasil e desenvolvimento do teste one-step RT-PCR para ...
Zika Virus Capsid ProteinZika Virus Capsid Protein
GenWay provides the Zika Virus Capsid Protein for various research applications. The Zika virus is a member of the virus family Flaviviridae and the genus Flavivirus. The Zika Virus Capsid Protein is offered along with other various Zika virus products.
Genomics and evolution of Aedes-borne flaviviruses - NERC Open Research ArchiveGenomics and evolution of Aedes-borne flaviviruses - NERC Open Research Archive
We analysed the complete coding sequences of all recognized species of Aedes-borne flavivirus, including previously uncharacterized viruses within the yellow fever virus (YFV), Spondweni virus (SPOV) and dengue virus (DENV) groups. Two major phylogenetic lineages were revealed: one included the YFV and Entebbe bat virus groups, and the other included the DENV, SPOV and Culex-borne flavivirus groups. This analysis supported previous evidence that Culex-borne flaviviruses have evolved from ancestral Aedes-borne viruses. However, the topology at the junction between these lineages remains complex and may be refined by the discovery of viruses related to the Kedougou virus. Additionally, viral evolution was found to be associated with the appearance of new biological characteristics; mutations that may modify the envelope protein structure were identified for seven viruses within the YFV group, and an expansion of host-vector range was identified in the two major evolutionary lineages, which in turn ...
Dimerization of flavivirus NS4B protein | MetaDimerization of flavivirus NS4B protein | Meta
Flavivirus replication is mediated by a complex machinery that consists of viral enzymes, nonenzymatic viral proteins, and host factors. Many of the nonenzymatic viral proteins, such as NS4B, are associated with the endoplasmic reticulum membrane. How these membrane proteins function in...read more ...
Zika virus: What is it? Symptoms, prevention, Treatment - HealthFoodInfo.OrgZika virus: What is it? Symptoms, prevention, Treatment - HealthFoodInfo.Org
People in this world are posing many health issues and different serious viruses which are providing harms to the human beings and also to the animals. Moreover, in this world with the development in the human brains, discoveries are often unlocking the conditions and seriousness of the various deceases and viruses, against which many time people need to gather together and fight simultaneously. This situation has already been occurred in relation to the cases of many deceases and viruses, like HIV/ AIDS, Cancer, etc. And now a day, as per experts research and ongoing circumstances, there is a major threat to the human community on this earths by the most terrifying Zika virus. As per experts analysis and meaning to this term, this Zika Virus (ZIKV) is a member of the virus family flaviviridae and the genus Flavivirus, which is transmitted by daytime- active Aedes mosquitoes. The name Zika Virus is brought from the Zika Forest of Uganda, where this virus was first isolated in the year ...
Detection of transfusion-associated hepatitis caused by non-A, non-B, non-C flavivirus.<...Detection of transfusion-associated hepatitis caused by non-A, non-B, non-C flavivirus.<...
TY - JOUR. T1 - Detection of transfusion-associated hepatitis caused by non-A, non-B, non-C flavivirus.. AU - Takács, M.. AU - Berencsi, G.. AU - Mezey, I.. AU - Brojnás, J.. AU - Barcsay, E.. AU - Garamvölgyi, E.. AU - Hütter, E.. AU - Ferenczi, E.. AU - Pipirász, E.. AU - Hollós, I.. PY - 1994. Y1 - 1994. N2 - Sera of patients suffering from acute hepatitis, and different forms of chronic hepatitis were found to be reactive to reagents prepared from the yellow fever virus (YF) vaccine strain. Serum samples of 1974 patients were tested, and 133 of them were positive. Hepatitis C virus specific antibodies were absent from the majority of them. The frequency of antibodies to other flaviviruses (tick-borne encephalitis, West Nile) and hepatitis B virus markers was similar to that measured among the population in Hungary positive for any of the surrogate markers of hepatitis infections. Results of both immunofluorescence tests, and Western blots suggest that there is a non-A, non-B, non-C ...
Zika Virus Testing in Pregnant Women: New CDC Recommendations - Lab Testing MattersZika Virus Testing in Pregnant Women: New CDC Recommendations - Lab Testing Matters
As a result of increasing knowledge about the Zika virus, the CDC updated their recommendations. The changes are based on declining trends in the number of reported cases of Zika virus infection in the Americas, emerging evidence on prolonged detection of Zika IgM antibodies, and new limitations for interpreting serologic tests during pregnancy. IgM is most likely to be detected in the first 12 weeks after infection but may persist beyond 12 weeks in some infected individuals, limiting the ability of testing to determine whether an infection occurred during or prior to pregnancy. False positive results and cross-reactivity with other flaviviruses can occur with IgM assays. Therefore, it is important to ascertain whether a woman had exposure to flaviviruses other than Zika virus before the current pregnancy because a positive IgM result might have been caused by cross-reactivity from a previous flavivirus exposure. Given the possibility of a false positive result, laboratory test results should ...
Zika virus | Britannica.comZika virus | Britannica.com
Zika virus: Zika virus, infectious agent of the genus Flavivirus in the family Flaviviridae. Zika virus was first isolated in 1947 from a rhesus monkey that had been caged in the canopy
The UTMB Newsroom | UTMB Health | UTMB.eduThe UTMB Newsroom | UTMB Health | UTMB.edu
Zika virus is a mosquito-borne disease that is a member of the flavivirus group. This group includes several other viruses that cause serious, sometimes life-threatening diseases such as yellow fever, dengue fever, West Nile and Japanese encephalitis.. Zika virus has been known for decades to circulate in Africa and Asia and more recently in the Pacific Islands, but very few cases of human disease were documented before 2007. In May of 2015, the first infections were confirmed in Brazil. Since then, millions people have been infected with Zika virus. Zika is now confirmed to be circulating in several countries and territories in the Americas including Mexico and is probably present in even more. The list of countries where Zika virus has been identified can change with new cases identified. What are the symptoms of Zika virus? In children and adults, Zika virus infection is generally mild - some develop flu-like symptoms, joint pain, eye inflammation and red rashes, while other people may not ...
Zika Virus on a Spreading Spree: what we now know that was unknown in the 1950s | Virology Journal | Full TextZika Virus on a Spreading Spree: what we now know that was unknown in the 1950's | Virology Journal | Full Text
Zika virus (ZIKV) is a mosquito-borne flavivirus that is transmitted through the bite of Aedes spp mosquitoes and less predominantly, through sexual intercourse. Prior to 2007, ZIKV was associated with only sporadic human infections with minimal or no clinical manifestations. Recently the virus has caused disease outbreaks from the Pacific Islands, the Americas, and off the coast of West Africa with approximately 1.62 million people suspected to be infected in more than 60 countries around the globe. The recent ZIKV outbreaks have been associated with guillain-barré syndrome, congenital syndrome (microcephaly, congenital central nervous system anomalies), miscarriages, and even death. This review summarizes the path of ZIKV outbreak within the last decade, highlights three novel modes of ZIKV transmission associated with recent outbreaks, and points to the hallmarks of congenital syndrome. The review concludes with a summary of challenges facing ZIKV research especially the control of ZIKV infection in
Scientists solve first flavivirus structure | EurekAlert! Science NewsScientists solve first flavivirus structure | EurekAlert! Science News
Scientists now have a way to target a number of insect-borne diseases, including dengue, West Nile, yellow fever and St. Louis encephalitis. Researchers from Purdue University and the California Institute of Technology have solved the three-dimensional structure of the dengue virus, providing the first detailed view of a virus from the flavivirus family. The findings open new avenues for developing vaccines and antiviral compounds to target diseases caused by flaviviruses.
Zika Virus: Quantification, Propagation, Detection, and Storage - Current ProtocolsZika Virus: Quantification, Propagation, Detection, and Storage - Current Protocols
Zika virus (ZIKV), belonging to the family Flaviviridae, genus Flavivirus, is an arthropod‐borne virus that was first discovered from the Zika forest in Uganda in 1947
JCI Insight - Reciprocal immune enhancement of dengue and Zika virus infection in human skinJCI Insight - Reciprocal immune enhancement of dengue and Zika virus infection in human skin
Dengue virus (DENV) and Zika virus (ZIKV) are closely related mosquito-borne flaviviruses that co-circulate in tropical regions and constitute major threats to global human health. Whether preexisting immunity to one virus affects disease caused by the other during primary or secondary infections is unknown but is critical in preparing for future outbreaks and predicting vaccine safety. Using a human skin explant model, we show that DENV-3 immune sera increased recruitment and infection of Langerhans cells, macrophages, and dermal dendritic cells following inoculation with DENV-2 or ZIKV. Similarly, ZIKV immune sera enhanced infection with DENV-2. Immune sera increased migration of infected Langerhans cells to the dermis and emigration of infected cells out of skin. Heterotypic immune sera increased viral RNA in the dermis almost 10-fold and reduced the amount of virus required to infect a majority of myeloid cells by 100- to 1000-fold. Enhancement was associated with cross-reactive IgG and ...
Drug development experts at Emory join fight against Zika virus - Georgia BioDrug development experts at Emory join fight against Zika virus - Georgia Bio
Drug Innovation Ventures at Emory (DRIVE) and the Emory Institute for Drug Development (EIDD) have launched an effort to identify and develop antivirals to treat the infection caused by Zika virus. There are currently no treatments or vaccines against the virus, which has been declared a public health emergency and is linked to birth defects.. According to George Painter, PhD, CEO of DRIVE and director of EIDD, "for the past three years, we have been synthesizing and developing antivirals against alphaviruses, such as chikungunya, and flaviviruses, such as dengue. Since Zika is a flavivirus in the same family as dengue and hepatitis C, we can apply what we have learned working on alphaviruses and flaviviruses, as well as from our past success with treatments for HIV, hepatitis B, hepatitis C and herpes viruses, in our search for an effective drug.". "We are making progress developing a Zika RNA Polymerase Assay," says Abel De La Rosa, PhD, chief scientific officer of DRIVE and EIDD. "This assay ...
Antibodies Elicited by an NS1-Based Vaccine Protect Mice Against Zika Virus - PubMedAntibodies Elicited by an NS1-Based Vaccine Protect Mice Against Zika Virus - PubMed
Zika virus is a mosquito-borne flavivirus which can cause severe disease in humans, including microcephaly and other congenital malformations in newborns and Guillain-Barré syndrome in adults. There are currently no approved prophylactics or therapeutics for Zika virus; the development of a safe and …
Welcome to CDC stacks | Zika virus disease in the United States, 2015-2016 [as of March 30, 2016] - 38769 | Stephen B. Thacker...Welcome to CDC stacks | Zika virus disease in the United States, 2015-2016 [as of March 30, 2016] - 38769 | Stephen B. Thacker...
On March 18, 2016, this report was posted as an MMWR Early Release on the MMWR website. : Zika virus is an emerging mosquito-borne flavivirus. Recent outbreaks of Zika virus disease in the Pacific Islands and the Region of the Americas have identifie ...
Structure and Function of Flaviviruses - Michael RossmannStructure and Function of Flaviviruses - Michael Rossmann
Flaviviruses are major human pathogens. They include West Nile, yellow fever and dengue viruses. These viruses cause millions of deaths each year in temperate a...
Zika virus structure, animation - Stock Video Clip K005/6534 - Science Photo LibraryZika virus structure, animation - Stock Video Clip K005/6534 - Science Photo Library
Animation of the structure of a Zika virus particle. The virus has an outer coat or envelope, studded with glycoproteins that help it bind to its target cell. Inside this is the protein capsid (red), which encloses the genetic material. This virus causes Zika fever, and is spread by the bites of infected mosquitoes. The disease originated in tropical east Africa, and was first detected in Uganda in 1947. Since April 2015 there has been a significant outbreak in South America, which started in Brazil and is spreading rapidly. The World Health Organisation stated it expected the disease to have spread to the majority of the Americas by the end of 2016. There have been reports of sexual transmission of the virus, and also of birth defects in pregnant women who become infected, with a rise in the rate of microcephaly being linked to the disease. Zika virus is a flavivirus, related to yellow fever. Each virus particle is around 50 nanometres in diameter. - Stock Video Clip K005/6534
Zika viral diseases: 80% can heal itself | HubPagesZika viral diseases: '80% can heal itself ' | HubPages
Zika virus /ˈziːkə, ˈzɪkə/ (ZIKV) is a member of the virus family Flaviviridae and the genus Flavivirus. It is spread by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus.
Wadsworth Center Graduates its Third Class of Master of Science in Laboratory Sciences StudentsWadsworth Center Graduates its Third Class of Master of Science in Laboratory Sciences Students
Joshua Rose. Joshua Roses capstone work was performed in the Arbovirology Laboratory [5], under the guidance of Dr. Alex Ciota [6] and Dr. Laura Kramer [7]. He utilized a unique flavivirus model to investigate genetic differences contributing to pathogenic emergence within vertebrate hosts, increasing the understanding of flavivirus evolution. With public health threats like Zika virus rapidly emerging, insights from his project will be helpful in understanding the capabilities of flaviviruses within vertebrate hosts. His project will also provide insights for antiviral drug design and vector control measures, giving the scientific community new potential "targets" to aim for when it comes to combating flaviviruses.. "Ultimately, I want my research to benefit the health of the public," Rose said. "I find great satisfaction in helping others and couldnt imagine a more suitable career for myself than that of a research scientist.". Rose earned his bachelors degree in biochemistry with a minor ...
PublicationsPublications
in april, 1975, an epidemic of human encephalitis was detected in several counties in the state of são paulo, brazil; the epidemic continued into 1976. a virus was isolated from central nervous system (cns) tissues of a 39-year-old male who died on december 8, 1975; the virus was found to be a new flavivirus for which the name rocio virus is proposed. nine further isolations of rocio virus were obtained from cns tissues of 17 patients who died with clinical symptoms of encephalitis. isolations o ...
The Viral Polymerase Inhibitor 7-Deaza-2-C-Methyladenosine Is a Potent Inhibitor of In Vitro Zika Virus Replication and Delays...The Viral Polymerase Inhibitor 7-Deaza-2'-C-Methyladenosine Is a Potent Inhibitor of In Vitro Zika Virus Replication and Delays...
Zika virus (ZIKV) is an emerging flavivirus typically causing a dengue-like febrile illness, but neurological complications, such as microcephaly in newborns, have potentially been linked to this viral infection. We established a panel of in vitro assays to allow the identification of ZIKV inhibitor …
Zica Virus | Find Me A CureZica Virus | Find Me A Cure
Definition: Zika virus is a member of the virus family Flaviviridae and the genus Flavivirus, transmitted by daytime-active Aedes mosquitoes, such as A. aegypti
Zikas structure has been revealed, bringing scientists closer to a vaccine - Viral World News, Gossip, Entertainment And...Zika's structure has been revealed, bringing scientists closer to a vaccine - Viral World News, Gossip, Entertainment And...
Zika differs most from other flaviviruses at a spot thought to be crucial to the cellular break-in. At this site, a carbohydrate molecule - made of different sugars - sits on the viruss protein shell. The so-called glycosylation site where Zika differs actually protrudes from the shell of the virus. In other viruses, similar protrusions act like strangers offering candy, tricking the human cell into binding with the invader. Like other flaviviruses, Zika seems to have a unique smattering of amino acids around that area ...
March | 2017 | DNA metabolismMarch | 2017 | DNA metabolism
Flavivirus serostatus (i.e. dengue and JE) at baseline and safety data at each time point were summarized by vaccine group. The safety analysis set was defined for each dose as those children who received a vaccine; data were analyzed according to the vaccine received. Between. 14 August 2010 and 31 July 2012, 550 participants were enrolled and 468 completed the Selleck 3MA study (Fig. 2). The main reason for discontinuation was voluntary withdrawal. No child withdrew owing to an AE. Mean age at inclusion, BMI, and ratio of male:female were similar in the three groups (Table 1). All children except one were Asian. Before vaccination, 2 children (2.0%) in JE-CV Group, 18 children (9.1%) in MMR Group and 5 children (2.3%) in Co-Ad Group were flavivirus seropositive i.e. they presented with pre-existing antibodies against either JE or dengue virus. All groups had low seroprotection/seropositivity rates before vaccination for all antigens (JE, measles, mumps and rubella). Non-inferiority was ...
Structures of immature flavivirus particles - Fingerprint - Experts@MinnesotaStructures of immature flavivirus particles - Fingerprint - [email protected]
Ying Zhang, Jeroen Corver, Paul R. Chipman, Wei Zhang, Sergei V. Pletnev, Dagmar Sedlak, Timothy S. Baker, James H. Strauss, Richard J. Kuhn, Michael G. Rossmann ...
Gentaur Molecular :bio-gentaur \ Banzi Virus Lysate \ GEN0810093Gentaur Molecular :bio-gentaur \ Banzi Virus Lysate \ GEN0810093
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Emergence of Zika compounds the global burden of mosquito-borne virusesEmergence of Zika compounds the global burden of mosquito-borne viruses
Infectious Diseases in Children | Brazilian officials estimate that there have been at least 1.5 million cases of Zika virus infection since the current outbreak began in May 2015, according to WHO.This dramatic increase in Brazil — and throughout the Americas — coupled with the now-confirmed association between the virus and microcephaly forced the CDC to issue a travel warning advising pregnant women to avoid travel
Why is WHO freaked about Zika? | SydologyWhy is WHO freaked about Zika? | Sydology
On February 1st, 2016 World Health Organization (WHO) announced a Public Health Emergency of International Concern. Whats the deal with Zika? Is it hype? Should we panic? Zika Basics According to WHO and Center for Disease Control (CDC): ~ Zika is a mosquito-borne virus carried by many species belonging to the aedes genre of mosquitos, found…
CiNii Articles - YOSHIHIKO YOKOSECiNii Articles - YOSHIHIKO YOKOSE
16. ヒト肺癌におけるGlutathione Stransferase π form(GST-π)の免疫組織化学的検討(第48回日本肺癌学会関西支部会) （1988 ...
CiNii Articles - YOKOSE YOSHIHIKOCiNii Articles - YOKOSE YOSHIHIKO
DEMDA AYUMI , MORI YUKIO , YOKOSE YOSHIHIKO , UCHIDA KAZUHIKO , MURATA YOSHIAKI , MAKINO TAKAO , TSUTSUMI MASAHIRO , KONISHI YOICHI The annual proceedings of Gifu College of Pharmacy 35, 83, 1986-06-30 ...
Genus: Flavivirus - Flaviviridae - Positive Sense RNA Viruses - International Committee on Taxonomy of Viruses (ICTV)Genus: Flavivirus - Flaviviridae - Positive Sense RNA Viruses - International Committee on Taxonomy of Viruses (ICTV)
Figure 2.Flavivirus. Flavivirus genome organization (not to scale) and polyprotein processing. The virion RNA is about 11 kb. At the top is the viral genome with the structural and nonstructural protein coding regions and the 5′- and 3′-NCRs. Boxes below the genome indicate viral proteins generated by the proteolytic processing cascade. P, H, and R symbols indicate the localization of the NS3 protease, the NS3 RNA helicase, and the NS5 RdRp domains, respectively.. Both the 5′-NCR and the 3′-NCR contain RNA sequence motifs that are involved in viral RNA translation, replication and possibly packaging. Although RNA secondary structure and function of some elements are conserved, sequence composition, length and exact localization can vary considerably between different members of the genus, in particular between tick-borne and mosquito-borne flaviviruses. In some cases, the 3′-NCR of tick-borne encephalitis virus, for example, contains an internal poly(A) tract. Viral infection induces ...
Flavivirus encephalitis | Radiology Reference Article | Radiopaedia.orgFlavivirus encephalitis | Radiology Reference Article | Radiopaedia.org
Flavivirus encephalitis include a number of entities which characteristically involve the basal ganglia and thalami. Clinical presentation Typically these conditions present with a prodrome which is nonspecific but indicative of a viral infect...
Flavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses | mBioFlavivirus Infection Uncouples Translation Suppression from Cellular Stress Responses | mBio
Flaviviruses were proposed to prevent rather than to induce host translation shutoff (36, 37, 40). Several technical aspects might explain this difference from our findings, including the use of nonhuman cells, different DENV serotype 2 strains, and time points p.i. chosen for analysis. Of note, polysome profiles of Huh7 cells infected with the DENV serotype 2 Bangkok strain 16681 (40) revealed in our experiments a similar translation repression to that induced by DENV1 infection (see Fig. 1F for reference), which also correlated with reduced cytopathic effect in Huh7 cells (data not shown). Analyses of human cells early p.i. by polysome profiling identified host cell translation repression as a new feature of flavivirus infection.. Flaviviruses actively block eIF2α-mediated stress response at different levels, supposedly to avoid the associated host translation suppression. First, WNV and DENV inhibit SG formation in nonhuman cells (36). Consistently, we observed that DENV and WNV, as well as ...
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Mosquitoes collected in northern Italy were screened for flavivirus RNA. Positive amplicons were sequenced and found most similar to insect flavivirus (ISF), Usutu virus (USUV) and surprisingly also to Japanese encephalitis virus (JEV). The sequence (167 bp), obtained from one pool of Culex pipiens, was found identical to JEV strains from bats in China. Unfortunately additional sequence data or virus isolations were not obtained in this study. Confirmation of potential introduction of JEV to Italy and other European countries is urgently needed.
Zika FeverZika Fever
... is caused by Zika virus. Zika virus ( ZIKV ) is a member of the Flaviviridae virus family and the Flavivirus genus, transmitted by daytime- active Aedes mosquitoes, such as A. aegypti.. General. Zika virus is related to dengue, yellow fever, Japanese encephalitis, and West Nile viruses. The illness it causes is similar to a mild form of dengue fever, is treated by rest, and cannot yet be prevented by drugs or vaccines. There is a possible link between Zika fever and microcephaly in newborn babies by mother- to- child transmission, as well as a stronger one with neurological conditions in infected adults, including cases of the Gullain- Barre syndrome.. Along with other viruses in this family, Zika virus is enveloped and icosahedral and has a non segmented, single- stranded, positive sense RNA genome. It is most closely related to the spondweni virus and is one of the two viruses in the spondweni virus Clade.. Transmission. Zika virus is transmitted by daytime- active mosquitoes and ...
Immunogenicity and Efficacy of Zika Virus Envelope Domain III in DNA, Protein, and ChAdOx1 Adenoviral-Vectored Vaccines. -...Immunogenicity and Efficacy of Zika Virus Envelope Domain III in DNA, Protein, and ChAdOx1 Adenoviral-Vectored Vaccines. -...
The flavivirus envelope protein domain III (EDIII) was an effective immunogen against dengue virus (DENV) and other related flaviviruses. Whether this can be applied to the Zika virus (ZIKV) vaccinology remains an open question. Here, we tested the efficacy of ZIKV-EDIII against ZIKV infection, using several vaccine platforms that present the antigen in various ways. We provide data demonstrating that mice vaccinated with a ZIKV-EDIII as DNA or protein-based vaccines failed to raise fully neutralizing antibodies and did not control viremia, following a ZIKV challenge, despite eliciting robust antibody responses. Furthermore, we showed that ZIKV-EDIII encoded in replication-deficient Chimpanzee adenovirus (ChAdOx1-EDIII) elicited anti-ZIKV envelope antibodies in vaccinated mice but also provided limited protection against ZIKV in two physiologically different mouse challenge models. Taken together, our data indicate that contrary to what was shown for other flaviviruses like the dengue virus, which has
Flavivirus VaccinesFlavivirus Vaccines
This report provides a competitor evaluation as of February 2016 in the field of vaccines on the market or in research & development for prevention of diseases caused by flavivirus such as Zika, Dengue, Chikungunya, West Nile, Yellow Fever, Japanese Encephalitis & Tick-Borne Encephalitis Virus.
CIENCIASMEDICASNEWS: Ahead of Print -Biologic Evidence Required for Zika Disease Enhancement by Dengue Antibodies - Volume 23,...CIENCIASMEDICASNEWS: Ahead of Print -Biologic Evidence Required for Zika Disease Enhancement by Dengue Antibodies - Volume 23,...
Zika virus, a member of the Flavivirus genus, is maintained in complex African zoonotic cycles, spilling from time to time into the Aedes aegypti mosquito urban transmission cycle (5). This spillover might be a very old phenomenon. Because many flaviviruses infect humans in Africa, it was logical to ask if antibodies to these viruses enhanced Zika virus infections. The answer obtained in vitro was affirmative (6). After having been first isolated in Africa in 1947, human Zika virus disease remained sparse and mild, with no reports of diverse clinical syndromes associated with infection (7). Nor was Zika disease reported from India or Southeast Asia, where Zika virus and DENV co-circulated, evidenced by detection of neutralizing antibodies in humans as early as 1954 (8-10). Zika virus was isolated from Ae. aegypti mosquitoes collected in rural Malaysia in 1966 (11). No alarms were raised in Asia until 2007, on the Yap Islands in the Western Pacific, when it was estimated that ≈900 cases of a ...
FlavivirusesFlaviviruses
The flaviviruses are a group of medically important viruses that are mainly transmitted by mosquitoes and ticks; they include dengue, Japanese encephalitis, St Louis encephalitis, tick‐borne encephalitis, West Nile and yellow fever species
Targeting Infectious Disease : Structural and functional studies of proteins from two RNA viruses and Mycobacterium tuberculosisTargeting Infectious Disease : Structural and functional studies of proteins from two RNA viruses and Mycobacterium tuberculosis
The recent emergence of a number of new viral diseases as well as the re-emergence of tuberculosis (TB), indicate an urgent need for new drugs against viral and bacterial infections.. Coronavirus nsp1 has been shown to induce suppression of host gene expression and interfere with host immune response. However, the mechanism behind this is currently unknown. Here we present the first nsp1 structure from an alphacoronavirus, Transmissible gastroenteritis virus (TGEV) nsp1. Contrary to previous speculation, the TGEV nsp1 structure clearly shows that alpha- and betacoronavirus nsp1s have a common evolutionary origin. However, differences in conservation, shape and surface electrostatics indicate that the mechanism for nsp1-induced suppression of host mRNA translation is likely to be different in the alpha- and betacoronavirus genera.. The Modoc virus is a neuroinvasive rodent virus with similar pathology as flavivirus encephalitis in humans. The flaviviral methyltransferase catalyses the two ...
GriffithGriffith
101 - 1,000 = High, 1,001 - 10,000 = Very High, ,10,000 = Extreme.. 28/Apr/2010: Hanwood had a high catch of 144, with 30 Culex annulirostris. Barren Box yielded a low catch. 27/Apr/2010: There were no seroconversions to either MVEV or KUNV from the bleeds taken on 8/Mar/2010, 15/Mar/2010, 22/Mar/2010, 29/Mar/2010 or 6/Apr. 21/Apr/2010: Hanwood had a high catch of 234, with 52 Culex annulirostris. Barren Box yielded a medium catch of73 and included 42 Culex annulirostris. 16/Apr/2010: The flavivirus isolate from Culex annulirostris trapped at Hanwood on the 22/Mar/10 was Kokobera virus. 14Apr/2010: Hanwood had a high catch of 230, with 159 Culex quinquefasciatus. Barren Box had a low catch. 8/Apr/2010: There was one flavivirus isolate from Culex annulirostris trapped at Hanwood on 29/Mar/2010. 6/Apr/2010: Mosquito numbers continue to be well above average and high from both sites. Hanwood trapped 107 mosquitoes, with 65 Culex annulirostris. Barren Box collected a total of 413 ...
Department of Health | Alphavirus and Flavivirus Laboratory Case Definition (LCD)Department of Health | Alphavirus and Flavivirus Laboratory Case Definition (LCD)
The Public Health Laboratory Network have developed a standard case definition for the diagnosis of diseases which are notifiable in Australia. This page contains the laboratory case definition for alphavirus and flavivirus infections.
Zika Virus Outside Africa - Volume 15, Number 9-September 2009 - Emerging Infectious Diseases journal - CDCZika Virus Outside Africa - Volume 15, Number 9-September 2009 - Emerging Infectious Diseases journal - CDC
Zika virus (ZIKV) is a flavivirus related to yellow fever, dengue, West Nile, and Japanese encephalitis viruses. In 2007 ZIKV caused an outbreak of relatively mild disease characterized by rash, arthralgia, and conjunctivitis on Yap Island in the southwestern Pacific Ocean. This was the first time that ZIKV was detected outside of Africa and Asia. The history, transmission dynamics, virology, and clinical manifestations of ZIKV disease are discussed, along with the possibility for diagnostic confusion between ZIKV illness and dengue.The emergence of ZIKV outside of its previously known geographic range should prompt awareness of the potential for ZIKV to spread to other Pacific islands and the Americas.
PLOS Neglected Tropical Diseases: Molecular Evolution of Zika Virus during Its Emergence in the 20th CenturyPLOS Neglected Tropical Diseases: Molecular Evolution of Zika Virus during Its Emergence in the 20th Century
Author Summary Zika fever is a mosquito-borne illness caused by a flavivirus. Human infections with Zika virus (ZIKV) could cause fever, malaise and cutaneous rash. Despite several ZIKV reports since 1947 when it was first isolated at Zika forest in Uganda, molecular evolution of ZIKV as an emerging agent remains poorly understood. Moreover, despite several ZIKV reports from Africa and Asia, few human cases were notified until 2007 when an epidemic took place in Micronesia. In West Africa, surveillance programs have reported periodic circulation of the virus since 1968. To help fill the gap in understanding ZIKV evolution, 43 ZIKV samples were analyzed. We focused on: (i) adaptive genetic changes including protein glycosylation patterns, (ii) phylogenetic relationship among isolates and their spatiotemporal patterns of spread across Africa and Asia and, (iii) dispersion among vertebrate reservoirs and invertebrate vector species. Our results indicated that ZIKV may have experienced recombination in
Flaviviridae - Flaviviridae - Positive Sense RNA Viruses - International Committee on Taxonomy of Viruses (ICTV)Flaviviridae - Flaviviridae - Positive Sense RNA Viruses - International Committee on Taxonomy of Viruses (ICTV)
The genomic RNA of all members of the family has a similar organization and is the viral mRNA found in infected cells. It contains a single long open reading frame (ORF) flanked by 5′- and 3′-terminal non-coding regions (NCRs) that form specific secondary structures required for genome replication and translation. Members of the genus Flavivirus, but not pestiviruses, hepaciviruses or pegiviruses produce a unique, subgenomic, small (0.3-0.5 kb) non-coding RNA that is derived from the 3′-NCR of genomic RNA (Lin et al., 2004) but which is essential for virus replication in cells and modulates pathogenicity in animals. Translation-initiation of genomic RNA is cap-dependent for members of the genus Flavivirus, whereas IRES elements are present in viruses of the other genera. Viral proteins are synthesized as part of a polyprotein that is co- and post-translationally cleaved by viral and cellular proteases. The structural proteins are contained in the N-proximal portion of this polyprotein and ...
Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia - UQ...Discovery and characterisation of a new insect-specific bunyavirus from Culex mosquitoes captured in northern Australia - UQ...
Insect-specific viruses belonging to significant arboviral families have recently been discovered. These viruses appear to be maintained within the insect population without the requirement for replication in a vertebrate host. Mosquitoes collected from Badu Island in the Torres Strait in 2003 were analysed for insect-specific viruses. A novel bunyavirus was isolated in high prevalence from Culex spp. The new virus, provisionally called Badu virus (BADUV), replicated in mosquito cells of both Culex and Aedes origin, but failed to replicate in vertebrate cells. Genomic sequencing revealed that the virus was distinct from sequenced bunyavirus isolates reported to date, but phylogenetically clustered most closely with recently discovered mosquito-borne, insect-specific bunyaviruses in the newly proposed Goukovirus genus. The detection of a functional furin cleavage motif upstream of the two glycoproteins in the M segment-encoded polyprotein suggests that BADUV may employ a unique strategy to ...
Article: Suramin inhibits Zika virus replication by interfering with virus attachment and release of infectious particles -...Article: Suramin inhibits Zika virus replication by interfering with virus attachment and release of infectious particles -...
Zika virus (ZIKV) is a mosquito-borne flavivirus that mostly causes asymptomatic infections or mild disease characterized by low-grade fever, rash, conjunctivitis, and malaise. However, the recent massive ZIKV epidemics in the Americas have also linked ZIKV infection to fetal malformations like microcephaly and Guillain-Barré syndrome in adults, and have uncovered previously unrecognized routes of vertical and sexual transmission. Here we describe inhibition of ZIKV replication by suramin, originally an anti-parasitic drug, which was more recently shown to inhibit multiple viruses. In cell culture-based assays, using reduction of cytopathic effect as read-out, suramin had an EC50 of ~40μM and a selectivity index of 48. In single replication cycle experiments, suramin treatment also caused a strong dose-dependent decrease in intracellular ZIKV RNA levels and a ,3-log reduction in infectious progeny titers. Time-of-addition experiments revealed that suramin inhibits a very early step of the ...
University of Vermont Employment Opportunities | Lab Research Technician | Print PreviewUniversity of Vermont Employment Opportunities | Lab Research Technician | Print Preview
Perform clinical virology and immunology assays of dengue and other flavivirus vaccines. Perform cell culture, immunology, and molecular biology assays to study the immune response to infectious pathogens for the Vaccine Testing Center. Perform clinical virology and immunogenicity assays of dengue and other flavivirus for clinical research trials. Compile, analyze and present data. Maintain laboratory and order supplies. Work with the Principal Investigator, laboratory manager, and other technicians to update protocols and develop new assays. Assist with writing of abstracts, methods, and preparation for presentations. May also perform immunology/cellular/molecular biology assays to study the immune response to other infectious pathogens. ...
Leveraging Established Fetal Primate Models to Expedite ZIKV Investigations - UC DavisLeveraging Established Fetal Primate Models to Expedite ZIKV Investigations - UC Davis
SUMMARY / ABSTRACTThe outbreak of Zika virus (ZIKV) and the alarming rise in fetal brain malformations highlight ZIKV as an urgentpublic health concern. ZIKV has recently met Shephards criteria for teratogenic classification because of thebrain anomalies reported. This application addresses the direct relationship between ZIKV infection and fetalbrain development using our established fetal primate model of intrauterine pathogenesis. The studies outlinedin this application leverage our prior discoveries and collaborative investigations on neural precursor cellfunction in relation to microglia in the fetal primate brain, and our expertise in primate development, imaging,virology, and immunology. Our track record and experienced team provides the means to pursue the goals ofthis proposal?understanding the mechanism(s) of ZIKV teratogenesis?and the steps necessary to progressto studies focused on interventions. Our goal is to determine how ZIKV alters cortical development bycapitalizing on our ...
Journal of Pediatrics Review - Zika Virus, Congenital Infection, and Neurologic Manifestations in Children: A Narrative ReviewJournal of Pediatrics Review - Zika Virus, Congenital Infection, and Neurologic Manifestations in Children: A Narrative Review
ZIKV laboratory tests should be considered in pregnant women who travel to regions with active ZIKV, as well as neonates born to these women (68, 69). These laboratory tests include RT-PCR for symptomatic patients or those with exposure to the virus within the past 2 weeks and serological assessment for people, exposed to the infection within the past 2 to 12 weeks. However, false-positive test results could be detected in patients infected with other flaviviruses; in fact, a negative test result does not ultimately reject ZIKV infection (70).. A specific test of ZIKV is the plaque reduction neutralization test, which measures virus-specific neutralizing antibodies (anti-ZIKV IgM antibodies) (71). ZIKV RT-PCR test should be performed on both infants serum and urine samples. Also, ZIKV DetectTM IgM ELISA should be simultaneously performed on the serum within the first 2 days of life. In general, laboratory tests for pregnant women with ZIKV infection include RT-PCR to detect ZIKV RNA in maternal ...
The American Journal of Tropical Medicine and Hygiene | Differential Neurovirulence of African and Asian Genotype Zika Virus...The American Journal of Tropical Medicine and Hygiene | Differential Neurovirulence of African and Asian Genotype Zika Virus...
Abstract. Although first isolated almost 70 years ago, Zika virus (ZIKV; Flavivirus, Flaviviridae) has only recently been associated with significant outbreaks of disease in humans. Several severe ZIKV disease manifestations have also been recently documented, including fetal malformations, such as microcephaly, and Guillain-Barré syndrome in adults. Although principally transmitted by mosquitoes, sexual transmission of ZIKV has been documented. Recent publications of several interferon receptor knockout mouse models have demonstrated ZIKV-induced disease. Herein, outbred immunocompetent CD-1/ICR adult mice were assessed for susceptibility to disease by intracranial (i.c.) and intraperitoneal (i.p.) inoculation with the Ugandan prototype strain (MR766; African genotype), a low-passage Senegalese strain (DakAr41524; African genotype) and a recent ZIKV strain isolated from a traveler infected in Puerto Rico (PRVABC59; Asian genotype). Morbidity was not observed in mice inoculated by the i.p. route with
World Health Orgaization Declares Zika Virus A Global Health Emergency - Latest Science NewsWorld Health Orgaization Declares Zika Virus A Global Health Emergency - Latest Science News
http://www.iflscience.com/sites/www.iflscience.com/files/styles/ifls_large/public/blog/%5Bnid%5D/zika.jpg?itok=IpBh1WLI http://www.scienceofwonder.org/world-health-orgaization-declares-zika-virus-a-global-health-emergency/ The World Health Organization (WHO) declared the Zika virus an international health emergency on Monday,a rare moveprompted by the explosive spread of the mosquito-borne virus. The Zika virus is now in more than 20 countries and territories in the Americas, with no effective vaccine or treatmet yet developed. Although most cases of the…
Differential Dominance Of Immune Response In Flaviviral Infections | 23703Differential Dominance Of Immune Response In Flaviviral Infections | 23703
Although closely related in structure flavivirus infections in humans have a vast range of tissue trophism and clinical syndromes starting from mild fever to h...
Brazil Deploys 200,000 Troops to Combat Zika Virus - Exchanging ideas and more…Brazil Deploys 200,000 Troops to Combat Zika Virus - Exchanging ideas and more…
Soldiers will be making house-to-house visits to spread awareness of the mosquito-borne virus. Brazil is deploying more than 200,000 soldiers to battle the Zika virus, which is believed to cause severe birth defects, the health ministry announced Wednesday. The troops will make house-to-house visits to spread awareness of the mosquito-borne virus, handing out leaflets…
Zika Virus Disease (ZIKV) Infographics, Language-tailored: Innovations and Good Practices in Integrated Disease Surveillance...Zika Virus Disease (ZIKV) Infographics, Language-tailored: Innovations and Good Practices in Integrated Disease Surveillance...
Zika Virus Disease (ZIKV) Infographics, Language-tailored: Innovations and Good Practices in Integrated Disease Surveillance Programme Kashmir, India 2016,
Zika Virus Disease (ZIKV) Infographics, Language-tailored: Innovations and Good Practices in Integrated Disease Surveillance...Zika Virus Disease (ZIKV) Infographics, Language-tailored: Innovations and Good Practices in Integrated Disease Surveillance...
The Zika virus (ZIKV) epidemics, declared by WHO as a global public health emergency in 2016, captured an unprecedented attention of the general publi..
Zika Virus Double check Realtime PCRZika Virus Double check Realtime PCR
Zika virus is a mosquito borne flavivirus. This RNA virus was discovered long ago (1948) in Africa. It is transmitted through mosquitoes Aedes. This virus is now infecting the population in American countries like Mexico, Brazil, Colombia , FR340 real time PCR kit for detection in different samples: nasal swabs, plasma, serum, stool, nasopharnygeal swabs, respiratory tract samples.
Supplementary Materialscells-08-01444-s001 - Intestinal secretion of immunoglobulins and antibodiesSupplementary Materialscells-08-01444-s001 - Intestinal secretion of immunoglobulins and antibodies
Supplementary Materialscells-08-01444-s001. wellness concern world-wide [1,2,3,4]. Latest ZIKV global outbreaks, with Brazil on the epicentre, highlighted what sort Mouse monoclonal to CD59(PE) of previously neglected flavivirus can turn into a severe threat for human being health. While human being ZIKV infections remained only sporadic and with a limited impact for decades [5,6,7,8], recent outbreaks exposed that ZIKV caused clusters of severe congenital and neurological abnormalities in babies and peripheral nervous system impairments in adults [9,10,11,12]. Considering the dramatic increase of severe human being cases, strategies to efficiently control this disease, either in terms of antiviral treatments or vaccines, are urgently needed and a granted requirement for more considerable studies. Flaviviruses contain a genomic single-stranded positive RNA encoding a single large polyprotein that is consequently cleaved by cellular and viral proteases into three structural proteins (C, prM/M and ...
WHO | Zika virusWHO | Zika virus
Zika virus is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in rhesus monkeys through a monitoring network of sylvatic yellow fever.
flavivirus | This Week in Virology - Part 2flavivirus | This Week in Virology - Part 2
In the second of two shows recorded at the University of North Carolina in Chapel Hill, Vincent meets up with faculty members to talk about how they got into science, their research on RNA viruses, and what they would be doing if they were not scientists.. ...
Scientists announce important Zika milestone: First vaccine ready for human trials - NextBillionScientists announce important Zika milestone: First vaccine ready for human trials - NextBillion
Since the World Health Organization declared Zika a public health emergency in February, teams of scientists from all over the world have been attacking the virus from many different angles. They have made advances in understanding the structure of the virus, the historical path of the mosquitoes that carry it and the risk to babies still in the womb - important knowledge, but of little immediate practical use.. Now, the discoveries have finally led to something that might be able to stop the pathogen: a vaccine.. Pennsylvania vaccine maker Inovio Pharmaceuticals and South Koreas GeneOne Life Sciences said Monday that they had received approval from U.S. regulators to start testing a DNA vaccine, known as GLS-5700, on humans. The early-stage study will include 40 healthy subjects. It is primarily designed to assess the safety of the vaccine but will also measure the immune response generated by the injection. Zika, part of the flavivirus family of viruses that includes West Nile, dengue and ...
Zika virus spreads fear among pregnant BraziliansZika virus spreads fear among pregnant Brazilians
By Anthony Boadle RECIFE, Brazil (Reuters) - For scores of women in the epicenter of the Zika outbreak in Brazil, the joy of pregnancy has given way to fear. In the sprawling coastal city of Recife, panic has struck maternity wards since Zika - a mosquito-borne virus first detected in the Americas
State health officials monitoring babies born to Zika mothers - WFSB 3 ConnecticutState health officials monitoring babies born to Zika mothers - WFSB 3 Connecticut
Connecticuts health department said it is monitoring dozens of babies either born or currently living in the state to mothers who tested positive for the Zika virus for Flavivirus.
As I See It: A Mosquito-borne Virus Has a SongAs I See It: A Mosquito-borne Virus Has a Song
The song also gives a "shout out to pregnant ladies" to protect themselves. Although Jamaica has so far confirmed only one case of the Zika virus, the government is telling women to delay getting pregnant for the next six to 12 months because of a possible link between Zika infection in pregnant women and microcephaly. ...
Zika virus (ZIKV)Zika virus (ZIKV)
Learn more about how the Roche diagnostics Zika test is enabling blood screening that helps healthcare authorities respond to challenging medical emergencies
Vaginal Probiotics Could Slow Zika & Other STD Infections « Invisiverse :: WonderHowToVaginal Probiotics Could Slow Zika & Other STD Infections « Invisiverse :: WonderHowTo
We might think of Zika as a mosquito-borne virus that effects developing fetuses, but, it also can be passed through sex by either a man or a woman, just like herpes and other STD viruses. New research has shown that vaginal bacteria can inhibit sexually transmitted Zika virus and Herpes Simplex Virus-2 in women.
Adaptive Changes in an Animal Model of Micro-Encephaly | Springer for Research & DevelopmentAdaptive Changes in an Animal Model of Micro-Encephaly | Springer for Research & Development
As a result of pre-natal or early post-natal exposure to a variety of agents (viruses, irradiation and cytotoxins) humans may develop microencephaly, characterized by reduction of brain size (Crome &...
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Advantages of testing for Zika virus (ZIKV) in urine have been reported, such as the persistence of ZIKV in this type of specimen for up to 20 days after ZIKV disease onset. We investigate 61 patients in the first 5 days post-symptom onset and find more patients testing positive for ZIKV in plasma samples (n=46), than in corresponding urine samples (n=37). For patients respectively testing positive in both plasma and urine (n=28), respective viral loads appeared similar.
PROGRAMACIÓN PARA SEGUNDO CICLO DE INFANTIL by Rocio Feal Rodriguez (eBook) - LuluPROGRAMACIÓN PARA SEGUNDO CICLO DE INFANTIL by Rocio Feal Rodriguez (eBook) - Lulu
Buy PROGRAMACIÓN PARA SEGUNDO CICLO DE INFANTIL by Rocio Feal Rodriguez (eBook) online at Lulu. Visit the Lulu Marketplace for product details, ratings, and reviews.
Cabañas El Rocio (Colon, Arjantin) - Otel Yorumları - TripAdvisorCabañas El Rocio (Colon, Arjantin) - Otel Yorumları - TripAdvisor
Cabañas El Rocio, Colon: Tarafsız yorumları okuyun, gerçek gezgin fotoğraflarına bakın. TripAdvisorın interaktif haritasını kullanarak konuma ve yakındaki restoran ve gezilecek yer seçeneklerine göz atın. Konaklamanız için fiyatları karşılaştırın ve en iyi teklifi alın.
Virus del Zika - Viquipèdia, lenciclopèdia lliureVirus del Zika - Viquipèdia, l'enciclopèdia lliure
El primer cas conegut de febre del Zika es va documentar en un macaco rhesus (Macaca mulatta) "sentinella" (un animal en captivitat controlada i sotmès a vigilància analítica) en un arbre del bosc de Zika, Uganda, el 1947, per un equip de científics britànics dirigits pel Dr. Haddow. Es van notificar també alguns brots a lÀfrica tropical i en algunes zones del sud-est asiàtic.[30] El primer estudi sobre embrions de pollastre infectats amb ZIKV fou publicat el 1952.[31]. Lany 1956, Boorman i Porterfield demostraren la seva transmissió per mosquits. Els moviments de tropes ocorreguts durant la IIGM (campanya de Birmània) podrien ser lorigen de lexpansió del virus de lÀfrica a lÀsia.[32] El 1978 es va descriure un petit brot de febre aguda a Indonèsia a causa de la infecció pel virus del Zika. Entre 1947 i 2006, varen ser identificats uns 20 casos dinfecció per ZIKV,[33] si bé les anàlisis actuals danticossos IgM fetes a mostres de sang antigues corresponents a malalts ...
Improved Zika Vaccine Produced by Tobacco Plant extract | SBYIRImproved Zika Vaccine Produced by Tobacco Plant extract | SBYIR
By Rideeta Raquib 19 The Zika virus (ZIKV) is a deadly virus transmitted through mosquito bites that at its climax has infected more than 1.5 million people in Brazil in 2015. The virus continues to spread with approximately 200 ZIKV cases reported in the United States this year. In order to combat the epidemic, new…
modoc county | KCETmodoc county | KCET
The weekends heavy rains may have put a damper on an annual coyote killing contest centered in the Modoc County town of Adin, but the close of the event took an unexpected turn anyway: with an alleged assault by the contests main sponsor on an oppone.... Read More. ...
US enrolls volunteers in large test of possible Zika vaccine | WTNH Connecticut NewsUS enrolls volunteers in large test of possible Zika vaccine | WTNH Connecticut News
U.S. health officials have begun enrolling volunteers for critical next-stage testing of an experimental vaccine to protect against Zika, the mosquito-borne virus that can cause devastating birth defects in pregnant women.
Why China Should be Concerned About Zika | Council on Foreign RelationsWhy China Should be Concerned About Zika | Council on Foreign Relations
Zika, a mosquito-borne virus that was first discovered in 1947 in Uganda, has resurged, spreading rapidly across the world. There were only thirteen…
Antigenic variationAntigenic variation
Dengue feverDengue fever
Dengue virusDengue virus
Japanese encephalitisJapanese encephalitis
West Nile virusWest Nile virus
Kyasanur Forest diseaseKyasanur Forest disease
Ticks of domestic animalsTicks of domestic animals
Spondweni virusSpondweni virus
FlavivirinFlavivirin
Yellow feverYellow fever
Powassan virusPowassan virus
Karshi virusKarshi virus
Tick-borne diseaseTick-borne disease
Parramatta River virusParramatta River virus
Tick-borne encephalitis virusTick-borne encephalitis virus
Transverse myelitisTransverse myelitis
Louping illLouping ill
SL2SL2
Entebbe bat virusEntebbe bat virus
West Nile feverWest Nile fever
PalmatinePalmatine
Usutu virusUsutu virus
Langat virusLangat virus
Fc receptorFc receptor
Veterinary virologyVeterinary virology
Social history of virusesSocial history of viruses
GB virus CGB virus C
Zika feverZika fever
Saint Louis encephalitisSaint Louis encephalitis
AnatomyOrganismsDiseasesAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaHealth CareGeographicals
FlavivirusYellow fever virusEncephalitis Viruses, Tick-BorneYellow FeverEncephalitis, Tick-BorneFlavivirus InfectionsWest Nile virusEncephalitis, St. LouisEncephalitis Virus, St. LouisDengue VirusIndianaEncephalitis Virus, JapaneseEncephalitis, JapaneseAlphavirusLaboratoriesAlphavirus InfectionsClinical Laboratory TechniquesPublic HealthHydranencephalyClassical swine fever virusClassical Swine FeverBorder disease virusDiarrhea Viruses, Bovine ViralBorder DiseasePestivirusJob SatisfactionVentilation-Perfusion RatioJob DescriptionLungJob ApplicationOccupational DiseasesEmploymentDengueSevere DengueNational Institute of Allergy and Infectious Diseases (U.S.)Hemorrhagic Fevers, ViralChikungunya virusWest Nile Fever
Flavivirus - WikipediaFlavivirus - Wikipedia
Scientists solve first flavivirus structure | EurekAlert! Science NewsScientists solve first flavivirus structure | EurekAlert! Science News
IJERPH | Free Full-Text | Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and...IJERPH | Free Full-Text | Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and...
Genomics and evolution of Aedes-borne flaviviruses - NERC Open Research ArchiveGenomics and evolution of Aedes-borne flaviviruses - NERC Open Research Archive
Flavivirus NS1: a multifaceted enigmatic viral protein | Virology Journal | Full TextFlavivirus NS1: a multifaceted enigmatic viral protein | Virology Journal | Full Text
EN (en)EN (en)
Development of a Rapid Solid Phase Immunoassay for the Detection of Flavivirus Antigens in Human Blood and Mosquito Vectors.Development of a Rapid Solid Phase Immunoassay for the Detection of Flavivirus Antigens in Human Blood and Mosquito Vectors.
Dimerization of flavivirus NS4B protein | MetaDimerization of flavivirus NS4B protein | Meta
Detection of transfusion-associated hepatitis caused by non-A, non-B, non-C flavivirus.<...Detection of transfusion-associated hepatitis caused by non-A, non-B, non-C flavivirus.<...
Structures of immature flavivirus particles - Fingerprint - Experts@MinnesotaStructures of immature flavivirus particles - Fingerprint - [email protected]
OPUS 4 | The role of integrins in flavivirus infectionOPUS 4 | The role of integrins in flavivirus infection
Structure and Function of Flaviviruses - Michael RossmannStructure and Function of Flaviviruses - Michael Rossmann
Zika virus: An emerging flavivirus | SpringerLinkZika virus: An emerging flavivirus | SpringerLink
Flavivirus structure and membrane fusion. - PubMed - NCBIFlavivirus structure and membrane fusion. - PubMed - NCBI
Envelope glycoprotein M, flavivirus (IPR000069) | InterPro | EMBL-EBIEnvelope glycoprotein M, flavivirus (IPR000069) | InterPro | EMBL-EBI
Flavivirus - definition of flavivirus by The Free DictionaryFlavivirus - definition of flavivirus by The Free Dictionary
Tick-borne flaviviruses. - PubMed - NCBITick-borne flaviviruses. - PubMed - NCBI
Flavivirus non-structural protein NS4A (IPR000404) | InterPro | EMBL-EBIFlavivirus non-structural protein NS4A (IPR000404) | InterPro | EMBL-EBI
Viruses | Free Full-Text | Flavivirus-Mosquito InteractionsViruses | Free Full-Text | Flavivirus-Mosquito Interactions
Flavivirus capsid hairpin cHP - WikipediaFlavivirus capsid hairpin cHP - Wikipedia
FlavivirusesFlaviviruses
Functional interplay among the flavivirus NS3 protease, helicase, and cofactors | SpringerLinkFunctional interplay among the flavivirus NS3 protease, helicase, and cofactors | SpringerLink
Frontiers | Seeking Flavivirus Cross-Protective Immunity | ImmunologyFrontiers | Seeking Flavivirus Cross-Protective Immunity | Immunology
Insulin Prevents Flavivirus Replication Within Mosquitoes - BioTechniquesInsulin Prevents Flavivirus Replication Within Mosquitoes - BioTechniques
Identification of two flavivirus-like genomes in the GB hepatitis agent. | PNASIdentification of two flavivirus-like genomes in the GB hepatitis agent. | PNAS
Department of Health | Alphavirus and Flavivirus Laboratory Case Definition (LCD)Department of Health | Alphavirus and Flavivirus Laboratory Case Definition (LCD)
Combinatorial Computational Approaches to Identify Tetracycline Derivatives as Flavivirus InhibitorsCombinatorial Computational Approaches to Identify Tetracycline Derivatives as Flavivirus Inhibitors
Viral Interference and Persistence in Mosquito-Borne FlavivirusesViral Interference and Persistence in Mosquito-Borne Flaviviruses
Monoclonal Anti-Flavivirus Group Antigen, Clone D1-4G2-4-15 (producedMonoclonal Anti-Flavivirus Group Antigen, Clone D1-4G2-4-15 (produced
Unraveling the Zika Virus Mystery: Lessons Learned from Another Flavivirus | HealthMapUnraveling the Zika Virus Mystery: Lessons Learned from Another Flavivirus | HealthMap
virus+v OR flavivirus Jobs | Juju - Smarter Job Search'virus+v' OR 'flavivirus' Jobs | Juju - Smarter Job Search
Molecular mechanisms of antibody-mediated neutralisation of flavivirus infection | Expert Reviews in...Molecular mechanisms of antibody-mediated neutralisation of flavivirus infection | Expert Reviews in...
A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in HorsesA High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses
Flavivirus Research, Vaccine Testing Center, Larner College of Medicine, UVMFlavivirus Research, Vaccine Testing Center, Larner College of Medicine, UVM
flavivirus | Antimicrobial Agents and Chemotherapyflavivirus | Antimicrobial Agents and Chemotherapy
Attenuation of the Langat tick-borne flavivirus by chimerization with mosquito-borne flavivirus dengue type 4 | PNASAttenuation of the Langat tick-borne flavivirus by chimerization with mosquito-borne flavivirus dengue type 4 | PNAS
Flavivirus VaccinesFlavivirus Vaccines
Difference between revisions of Flavivirus NS1 protein - microbewikiDifference between revisions of "Flavivirus NS1 protein" - microbewiki
Immune cross recognition of a conserved Flavivirus epitope (130.28) | The Journal of ImmunologyImmune cross recognition of a conserved Flavivirus epitope (130.28) | The Journal of Immunology
Pathogens | Free Full-Text | Role of Endoplasmic Reticulum-Associated Proteins in Flavivirus Replication and Assembly ComplexesPathogens | Free Full-Text | Role of Endoplasmic Reticulum-Associated Proteins in Flavivirus Replication and Assembly Complexes
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FlavivirusYellow fever virusEncephalitis Viruses, Tick-BorneYellow FeverEncephalitis, Tick-BorneFlavivirus InfectionsWest Nile virusEncephalitis, St. LouisEncephalitis Virus, St. LouisDengue VirusIndianaEncephalitis Virus, JapaneseEncephalitis, JapaneseAlphavirusLaboratoriesAlphavirus InfectionsClinical Laboratory TechniquesPublic HealthHydranencephalyClassical swine fever virusClassical Swine FeverBorder disease virusDiarrhea Viruses, Bovine ViralBorder DiseasePestivirusJob SatisfactionVentilation-Perfusion RatioJob DescriptionLungJob ApplicationOccupational DiseasesEmploymentDengueSevere DengueNational Institute of Allergy and Infectious Diseases (U.S.)Hemorrhagic Fevers, ViralChikungunya virusWest Nile Fever
West NileZikaVirusesInfectionsArbovirusesAntibodiesGenus flavivirusDifferent flavivirusesInfectionProteinsCharacterizationMosquitoesProteinVectorsTicksAntigensCross-reactivityIntegrinsHepatitisZIKVRefractoryInhibitionPathogenicGenesGenusFlaviviridaeProteinsGenomeDengue virusViralJournal of VirolYellow feverVirusEncephalitisPolyproteinAntigenicDiseasesSubunitDetectionCross-reactiveEnvelope proteinMonoclonalInsect-specific flavivirusAlphavirusesStructure and membraneVirologyLevels of flavivirusProtein of flavivirusesFormation of flavivirusEpidemics of flavivirusesNonstructural proteinKnown flavivirusesDifferentiationNeurotropic flavivirusesPathology
West Nile6
Zika2
Viruses7
Infections1
Arboviruses1
  • The known non-arboviruses of the flavivirus family reproduce in either arthropods or vertebrates, but not both, with one odd member of the genus affecting a nematode. (wikipedia.org)
Antibodies2
  • However, since the available broadly group-reactive MAB has been found to exhibit at least some degree of cross-competition, flavivirus type-specific detector MAB directed at sites distant from the group-reactive domain have been extensively used to evaluate the properties of potential flavivirus group-reactive capture antibodies. (dtic.mil)
  • Initial assays demonstrated the capacity of the YF-derived antibodies to capture prototype flaviviruses. (dtic.mil)
Genus flavivirus1
Different flaviviruses1
Infection4
  • The findings, published in Friday's (3/8) issue of the scientific journal Cell, provide the first detailed view of a flavivirus and offer structural information that can be used to unravel the processes that lead to viral infection. (eurekalert.org)
  • In the present study, murine cell lines lacking the expression of one or more integrin subunits were used to evaluate the involvement of different integrins in the flavivirus infection cycle. (uni-greifswald.de)
  • Our findings clearly demonstrate that β1, β3 and αVβ3 integrins do not act as flavivirus cellular receptor or attachment factor since their ablation does not completely abrogate flavivirus infection in the investigated cell lines. (uni-greifswald.de)
  • The results strongly indicate that the investigated integrins play an important role in flavivirus infection and might represent a novel host cell factor that enhances flavivirus replication. (uni-greifswald.de)
Proteins2
Characterization1
  • Attention has been directed at the identification and characterization of MAB with broad flavivirus group-reactive domain as determined by competitive binding assay with the expectation that such antibody combination will provide the best opportunity for antigen binding and therefore maximum assay sensitivity. (dtic.mil)
Mosquitoes1
  • The goal of our work has been the development of a generic flavivirus detection system and emphasis has been placed on an enzyme-linked immunoassay ELISA for the detection of DEN-infected mosquitoes. (dtic.mil)
Protein1
  • isoelectrofocusing of nonionic-detergent-disrupted flaviviruses separated the envelope glycoprotein of 53,000 to 58,000 daltons and the nucleocapsid protein of 14,000 daltons. (liverpool.ac.uk)
Vectors1
  • Development of a Rapid Solid Phase Immunoassay for the Detection of Flavivirus Antigens in Human Blood and Mosquito Vectors. (dtic.mil)
Ticks2
  • the isolation of saumarez reef virus, a new flavivirus, from bird ticks ornithodoros capensis and ixodes eudyptidis in australia. (liverpool.ac.uk)
  • strains of a new flavivirus, for which the name saumarez reef virus is proposed, were isolated from seabird ticks collected from four localities. (liverpool.ac.uk)
Antigens1
  • the buoyant densities of western equine encephalomyelitis virus (an alphavirus) and tick-borne encephalitis (tbe) virus (a flavivirus) antigens prepared by different methods were studied. (liverpool.ac.uk)
Cross-reactivity1
  • Results of both immunofluorescence tests, and Western blots suggest that there is a non-A, non-B, non-C hepatitis virus circulating among the Hungarian population, which possesses antigenic cross-reactivity with the yellow fever virus, but the identity to any of the known flaviviruses could not be verified yet. (elsevier.com)
Integrins3
Hepatitis1
ZIKV1
  • In contrast to that, loss of αVβ3 integrin in the MEF-αVβ3-/- cells showed a statistically significant decrease in WNV and USUV internalization while ZIKV, YFV-17D and LGTV internalization remained unaffected suggesting that αVβ3 integrin might be involved in the internalization process of at least some flaviviruses. (uni-greifswald.de)
Refractory1
  • A second model using Chinese hamster ovary cells (CHO-K1), a cell line that has been described to be refractory to some flaviviruses, were modified to express either αV (CHO-αV+/+) or β3 (CHO-β3+/+) integrin subunits. (uni-greifswald.de)
Inhibition1
Pathogenic1
  • He utilized a unique flavivirus model to investigate genetic differences contributing to pathogenic emergence within vertebrate hosts, increasing the understanding of flavivirus evolution. (wadsworth.org)
Genes1
Genus23
Flaviviridae1
Proteins16
Genome8
Dengue virus4
Viral9
Journal of Virol1
  • The study released this week online in the Journal of Virology describes how a single virus protein--NS5--from the tick-borne Langat flavivirus counteracts the natural ability of interferon to combat the virus. (rxpgnews.com)
Yellow fever3
Virus26
  • Recombinant subviral particles from tick-borne encephalitis virus are fusogenic and provide a model system for studying flavivirus envelope glycoprotein functions. (ebi.ac.uk)
  • Flaviviruses have been especially difficult to study in part because their properties make it difficult to produce the large amounts of undamaged particles needed for the high-resolution techniques used to study virus structures. (eurekalert.org)
  • To do these experiments, we need large amounts of undamaged virus, and the flaviviruses are difficult to culture," he says. (eurekalert.org)
  • Limited sequence identity with various isolates of hepatitis C virus and the relative positions of putative RNA helicases and RNA-dependent RNA polymerases in the predicted protein products of these molecules suggested that the GB agent contains two unique flavivirus-like genomes. (pnas.org)
  • The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes. (hindawi.com)
  • Langat virus (LGT) strain TP21 is the most attenuated of the tick-borne flaviviruses for humans. (pnas.org)
  • Even though LGT has low-level neurovirulence for humans, it, and its more attenuated egg-passage derivative, strain E5, exhibit significant neurovirulence and neuroinvasiveness in normal mice, albeit less than that associated with tick-borne encephalitis virus (TBEV), the most virulent of the tick-borne flaviviruses. (pnas.org)
  • RML's Sonja Best, Ph.D., who spearheaded the Langat virus work, says the group will continue to study tick-borne flaviviruses by examining the role and location of NS5 in Powassan virus. (rxpgnews.com)
  • National Institutes of Health (NIH) scientists have filled a research gap by developing a laboratory model to study ticks that transmit flaviviruses, such as Powassan virus. (nih.gov)
  • Powassan virus and the closely related deer tick virus are the only flaviviruses known to be spread by ticks in North America. (nih.gov)
  • Kunjin virus (KUN) is an Australian flavivirus closely related to other members of the Japanese encephalitis (JE) virus subgroup ( 32 ). (asm.org)
  • Indeed, most flavivirus antibodies are directed against the highly immunogenic envelope protein, which contains both flavivirus cross-reactive and virus-specific epitopes. (mdpi.com)
  • Two major phylogenetic lineages were revealed: one included the YFV and Entebbe bat virus groups, and the other included the DENV, SPOV and Culex-borne flavivirus groups. (nerc.ac.uk)
  • Researchers at The University of Queensland have developed a new chimeric virus platform suitable for use in flavivirus vaccine and diagnostic applications. (uniquest.com.au)
  • Tick-borne encephalitis virus (TBEV), a zoonotic flavivirus that occurs on the Eurasian continent and causes tick-borne encephalitis (TBE) in humans, is considered medically the most important arthropod vector transmitted virus in Europe. (biomedcentral.com)
  • The new EMNT assay combines the biological functional assessment of virus neutralization activity and the technical advantages of ELISA and, is simple, reliable, practical, and could be automated for high-throughput implementation in flavivirus surveillance studies and vaccine trials. (nih.gov)
  • Isolation of a new flavivirus related to Cell fusing agent virus (CFAV) from field-collected. (deepdyve.com)
  • Results from cross-neutralization tests suggested that, although the BLAST search indicated homology to CFAV, the virus isolated represented a new insect flavivirus. (deepdyve.com)
  • We propose this new flavivirus be designated Kamiti River virus (KRV). (deepdyve.com)
  • Flavivirus NS1 is a nonstructural protein involved in virus replication and regulation of the innate immune response. (pubmedcentralcanada.ca)
  • Flavivirus NS1 is a multifunctional protein shown to play a role in virus replication and assembly ( 13 , 15 , 18 , 20 ), as well as in the modulation of the innate immune response ( 6 , 7 , 24 ). (pubmedcentralcanada.ca)
  • It is proposed that attenuation of flaviviruses following passage in HeLa cells is dependant on the tropism of the virus. (surrey.ac.uk)
  • JE virus, a mosquitoborne flavivirus, is the most common vaccine-preventable cause of encephalitis in Asia. (cdc.gov)
  • Host cell cytoskeletons have been reported to have close contact with flaviviruses during virus entry, intracellular transport, replication, and egress process, although many detailed mechanisms are still unclear. (virosin.org)
  • Brault JB, Kudelko M, Vidalain PO, Tangy F, Despres P, Pardigon N (2011) The interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication. (virosin.org)
  • Detection of flavivirus seroconversions in sentinel chicken flocks across Australia provides an early warning of increased levels of Murray Valley encephalitis virus and Kunjin virus activity. (health.gov.au)
Encephalitis4
Polyprotein3
Antigenic4
Diseases6
  • The discovery may help scientists develop antiviral compounds and other strategies to target dengue and other diseases caused by flaviviruses, says Richard Kuhn, associate professor of biological sciences and lead author of the study. (eurekalert.org)
  • Moreover, cross-reactive epitopes presented in this work may be involved in dynamics of diseases caused by flaviviruses worldwide. (frontiersin.org)
  • This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases. (hindawi.com)
  • The unusual model involves culturing organs taken from Ixodes scapularis ticks and then infecting those organ cultures with flaviviruses, according to researchers at Rocky Mountain Laboratories, part of NIH's National Institute of Allergy and Infectious Diseases (NIAID). (nih.gov)
  • An understanding of flavivirus E-glycoprotein cross-reactive epitopes is therefore critical for improving public health responses to these serious diseases. (asm.org)
  • Flavivirus causes arthropod-borne severe diseases that sometimes lead to the death. (ncbs.res.in)
Subunit1
Detection3
  • Development of a Rapid Solid Phase Immunoassay for the Detection of Flavivirus Antigens in Human Blood and Mosquito Vectors. (dtic.mil)
  • The goal of our work has been the development of a generic flavivirus detection system and emphasis has been placed on an enzyme-linked immunoassay ELISA for the detection of DEN-infected mosquitoes. (dtic.mil)
  • Detection of transfusion-associated hepatitis caused by non-A, non-B, non-C flavivirus. (elsevier.com)
Cross-reactive1
Envelope protein4
Monoclonal1
Insect-specific flavivirus1
Alphaviruses1
Structure and membrane1
Virology1
Levels of flavivirus2
Protein of flaviviruses1
  • NS5 protein of flaviviruses contains motifs for methyltransferase (MT) and RNA-dependent RNA polymerase (RdRp) ( 25 , 26 , 34 ), and NS3 protein contains motifs for serine protease, nucleoside triphosphatase, and helicase ( 16 , 17 , 43 ). (asm.org)
Formation of flavivirus1
  • This review highlights the fact that the ER multiprotein complexes are crucial for the formation of flavivirus replication and assembly complexes, and the ER complexes could be considered as a target for developing successful broad-spectrum anti-flavivirus drugs. (mdpi.com)
Epidemics of flaviviruses2
  • Many areas of the world present both, vector fauna and geographical conditions compatible with co-circulation, importing, emergence, and epidemics of flaviviruses of different serocomplexes. (frontiersin.org)
  • Members of this family that infect humans frequently cause severe morbidity and mortality, and epidemics of flaviviruses continue to be a major public health concern worldwide. (asm.org)
Nonstructural protein1
Known flaviviruses1
Differentiation1
Neurotropic flaviviruses2
  • Thus, T cell signaling to microglia drives post-infectious cognitive sequelae that are associated with emerging neurotropic flaviviruses. (nature.com)
  • Therefore, it is suggested that the attenuation of flaviviruses following passage in HeLa cells provides a model system to examine the molecular basis of attenuation of non-neurotropic flaviviruses. (surrey.ac.uk)
Pathology1
View source for Zika virus - microbewiki
View source for Zika virus - microbewiki (microbewiki.kenyon.edu)
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Combinatorial Computational Approaches to Identify Tetracycline Derivatives as Flavivirus Inhibitors (journals.plos.org)
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Japanese Encephalitis Vaccine: Recommendations of the Advisory Committee on Immunization Practices | MMWR (cdc.gov)
Zika virus: An emerging flavivirus | SpringerLink
Zika virus: An emerging flavivirus | SpringerLink (link.springer.com)
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Japanese Encephalitis Vaccines (cdc.gov)
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Search Results (atcc.org)
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Updates on Brucellosis | IntechOpen (intechopen.com)
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Chagas Disease - Basic Investigations and Challenges | IntechOpen (intechopen.com)
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Human Papillomavirus | IntechOpen (intechopen.com)
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Understanding Tuberculosis - Deciphering the Secret Life of the Bacilli | IntechOpen (intechopen.com)
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Department of Health | Sentinel Chicken Surveillance Program in Australia, July 2003 to June 2004 (health.gov.au)
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Frontiers | Seeking Flavivirus Cross-Protective Immunity | Immunology (frontiersin.org)
Functional interplay among the flavivirus NS3 protease, helicase, and cofactors | SpringerLink
Functional interplay among the flavivirus NS3 protease, helicase, and cofactors | SpringerLink (link.springer.com)
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Identification of two flavivirus-like genomes in the GB hepatitis agent. | PNAS
Identification of two flavivirus-like genomes in the GB hepatitis agent. | PNAS (pnas.org)
Japanese encephalitis - Wikipedia
Japanese encephalitis - Wikipedia (en.wikipedia.org)
Frontiers | Complement Evasion Strategies of Viruses: An Overview | Microbiology
Frontiers | Complement Evasion Strategies of Viruses: An Overview | Microbiology (frontiersin.org)
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Update: Interim Guidance for Health Care Providers Caring for Pregnant Women with Possible Zika Virus Exposure - United States ... (cdc.gov)
Flavivirus NS1 protein - microbewiki
Flavivirus NS1 protein - microbewiki (microbewiki.kenyon.edu)
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Ted Pierson, Ph.D. | NIH: National Institute of Allergy and Infectious Diseases (niaid.nih.gov)
Scientists Develop Infection Model for Tick-borne Flaviviruses | NIH: National Institute of Allergy and Infectious Diseases
Scientists Develop Infection Model for Tick-borne Flaviviruses | NIH: National Institute of Allergy and Infectious Diseases (niaid.nih.gov)
Dr Adjanie Patabendige / Staff Profile / The University of Newcastle, Australia
Dr Adjanie Patabendige / Staff Profile / The University of Newcastle, Australia (newcastle.edu.au)
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Engineered Mosquitos Carry Antibodies For Dengue Virus - BioTechniques (biotechniques.com)
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On The Hunt For a Coronavirus Vaccine - BioTechniques (biotechniques.com)
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Closing the Loop on Genome Folding - BioTechniques (biotechniques.com)
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Biotech Recipes for Sustainable Food - BioTechniques (biotechniques.com)
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Israel Journal of Veterinary Medicine - Vol. 70(2) - Jun. 2015 | IVIS (ivis.org)
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Virology Proteomics News | Thermo Fisher Scientific (thermofisher.com)
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Powassan Virus News, Research (news-medical.net)