Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).
Adhesins, Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
Enterotoxigenic Escherichia coli
Strains of ESCHERICHIA COLI that produce or contain at least one member of either heat-labile or heat-stable ENTEROTOXINS. The organisms colonize the mucosal surface of the small intestine and elaborate their enterotoxins causing DIARRHEA. They are mainly associated with tropical and developing countries and affect susceptible travelers to those places.
Urinary Tract Infections
Filamentous or elongated proteinaceous structures which extend from the cell surface in gram-negative bacteria that contain certain types of conjugative plasmid. These pili are the organs associated with genetic transfer and have essential roles in conjugation. Normally, only one or a few pili occur on a given donor cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed, p675) This preferred use of "pili" refers to the sexual appendage, to be distinguished from bacterial fimbriae (FIMBRIAE, BACTERIAL), also known as common pili, which are usually concerned with adhesion.
Proline-Rich Protein Domains
Protein domains that are enriched in PROLINE. The cyclical nature of proline causes the peptide bonds it forms to have a limited degree of conformational mobility. Therefore the presence of multiple prolines in close proximity to each other can convey a distinct conformational arrangement to a peptide chain.
Uropathogenic Escherichia coli
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
P Blood-Group System
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Gene Expression Regulation, Bacterial
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Amino Acid Sequence
Salivary Proline-Rich Proteins
A family of proline-rich proteins that constitute the majority of the protein component of SALIVA. Salivary proline-rich proteins occur as acidic, basic and glycosylated basic proteins. They perform a variety of functions such as adhering to the acquired ENAMEL PELLICLE, acting as lubricants and precipitating TANNINS.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Salivary Proteins and Peptides
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Escherichia coli Vaccines
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Role of DnaK in in vitro and in vivo expression of virulence factors of Vibrio cholerae. (1/1658)The dnaK gene of Vibrio cholerae was cloned, sequenced, and used to construct a dnaK insertion mutant which was then used to examine the role of DnaK in expression of the major virulence factors of this important human pathogen. The central regulator of several virulence genes of V. cholerae is ToxR, a transmembrane DNA binding protein. The V. cholerae dnaK mutant grown in standard laboratory medium exhibited phenotypes characteristic of cells deficient in ToxR activity. Using Northern blot analysis and toxR transcriptional fusions, we demonstrated a reduction in expression of the toxR gene in the dnaK mutant strain together with a concomitant increase in expression of a htpG-like heat shock gene that is located immediately upstream and is divergently transcribed from toxR. This may be due to increased heat shock induction in the dnaK mutant. In vivo, however, although expression from heat shock promoters in the dnaK mutant was similar to that observed in vitro, expression of both toxR and htpG was comparable to that by the parental strain. In both strains, in vivo expression of toxR was significantly higher than that observed in vitro, but no reciprocal decrease in htpG expression was observed. These results suggest that the modulation of toxR expression in vivo may be different from that observed in vitro. (+info)
Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. (2/1658)The Vibrio cholerae genome contains a 5.4-kb pil gene cluster that resembles the Aeromonas hydrophila tap gene cluster and other type IV-A pilus assembly operons. The region consists of five complete open reading frames designated pilABCD and yacE, based on the nomenclature of related genes from Pseudomonas aeruginosa and Escherichia coli K-12. This cluster is present in both classical and El Tor biotypes, and the pilA and pilD genes are 100% conserved. The pilA gene encodes a putative type IV pilus subunit. However, deletion of pilA had no effect on either colonization of infant mice or adherence to HEp-2 cells, demonstrating that pilA does not encode the primary subunit of a pilus essential for these processes. The pilD gene product is similar to other type IV prepilin peptidases, proteins that process type IV signal sequences. Mutational analysis of the pilD gene showed that pilD is essential for secretion of cholera toxin and hemagglutinin-protease, mannose-sensitive hemagglutination (MSHA), production of toxin-coregulated pili, and colonization of infant mice. Defects in these functions are likely due to the lack of processing of N termini of four Eps secretion proteins, four proteins of the MSHA cluster, and TcpB, all of which contain type IV-A leader sequences. Some pilD mutants also showed reduced adherence to HEp-2 cells, but this defect could not be complemented in trans, indicating that the defect may not be directly due to a loss of pilD. Taken together, these data demonstrate the effectiveness of the V. cholerae genome project for rapid identification and characterization of potential virulence factors. (+info)
Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae. (3/1658)Salmonella typhimurium exhibits a distinct tropism for mouse enterocytes that is linked to their expression of type 1 fimbriae. The distinct binding traits of Salmonella type 1 fimbriae is also reflected in their binding to selected mannosylated proteins and in their ability to promote secondary bacterial aggregation on enterocyte surfaces. The determinant of binding in Salmonella type 1 fimbriae is a 35-kDa structurally distinct fimbrial subunit, FimHS, because inactivation of fimHS abolished binding activity in the resulting mutant without any apparent effect on fimbrial expression. Surprisingly, when expressed in the absence of other fimbrial components and as a translational fusion protein with MalE, FimHS failed to demonstrate any specific binding tropism and bound equally to all cells and mannosylated proteins tested. To determine if the binding specificity of Salmonella type 1 fimbriae was determined by the fimbrial shaft that is intimately associated with FimHS, we replaced the amino-terminal half of FimHS with the corresponding sequence from Escherichia coli FimH (FimHE) that contains the receptor binding domain of FimHE. The resulting hybrid fimbriae bearing FimHES on a Salmonella fimbrial shaft exhibited binding traits that resembled that of Salmonella rather than E. coli fimbriae. Apparently, the quaternary constraints imposed by the fimbrial shaft on the adhesin determine the distinct binding traits of S. typhimurium type 1 fimbriae. (+info)
Natural competence for DNA transformation by Legionella pneumophila and its association with expression of type IV pili. (4/1658)We have recently described the expression of two pili of different lengths on the surface of Legionella pneumophila (B. J. Stone and Y. Abu Kwaik, Infect. Immun. 66:1768-1775, 1998). Production of long pili requires a functional pilEL locus, encoding a type IV pilin protein. Since type IV pili in Neisseria gonorrhoeae are associated with competence for DNA transformation, we examined the competence of L. pneumophila for DNA transformation under conditions that allowed the expression of type IV pili. We show that L. pneumophila is naturally competent for DNA transformation by isogenic chromosomal DNA and by plasmid DNA containing L. pneumophila DNA. Many different L. pneumophila loci are able to transform L. pneumophila after addition of plasmid DNA, including gspA, ppa, asd, and pilEL. The transformation frequency is reduced when competing DNA containing either L. pneumophila DNA or vector sequences is added to the bacteria, suggesting that uptake-specific sequences may not be involved in DNA uptake. Competence for DNA transformation correlates with expression of the type IV pili, and a pilEL mutant defective in expression of type IV pili is not competent for DNA transformation. Complementation of the mutant for competence is restored by the reintroduction of a cosmid that restores production of type IV pili. Minimal competence is restored to the mutant by introduction of pilEL alone. We conclude that competence for DNA transformation in L. pneumophila is associated with expression of the type IV pilus and results in recombination of L. pneumophila DNA into the chromosome. Since expression of type IV pili also facilitates attachment of L. pneumophila to mammalian cells and protozoa, we designated the type IV pili CAP (for competence- and adherence-associated pili). (+info)
Environmental signals modulate ToxT-dependent virulence factor expression in Vibrio cholerae. (5/1658)The regulatory protein ToxT directly activates the transcription of virulence factors in Vibrio cholerae, including cholera toxin (CT) and the toxin-coregulated pilus (TCP). Specific environmental signals stimulate virulence factor expression by inducing the transcription of toxT. We demonstrate that transcriptional activation by the ToxT protein is also modulated by environmental signals. ToxT expressed from an inducible promoter activated high-level expression of CT and TCP in V. cholerae at 30 degrees C, but expression of CT and TCP was significantly decreased or abolished by the addition of 0.4% bile to the medium and/or an increase of the temperature to 37 degrees C. Also, expression of six ToxT-dependent TnphoA fusions was modulated by temperature and bile. Measurement of ToxT-dependent transcription of genes encoding CT and TCP by ctxAp- and tcpAp-luciferase fusions confirmed that negative regulation by 37 degrees C or bile occurs at the transcriptional level in V. cholerae. Interestingly, ToxT-dependent transcription of these same promoters in Salmonella typhimurium was relatively insensitive to regulation by temperature or bile. These data are consistent with ToxT transcriptional activity being modulated by environmental signals in V. cholerae and demonstrate an additional level of complexity governing the expression of virulence factors in this pathogen. We propose that negative regulation of ToxT-dependent transcription by environmental signals prevents the incorrect temporal and spatial expression of virulence factors during cholera pathogenesis. (+info)
Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. (6/1658)Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an important human pathogen. The production of several virulence factors by P. aeruginosa is controlled through two quorum-sensing systems, las and rhl. We have obtained evidence that both the las and rhl quorum-sensing systems are also required for type 4 pilus-dependent twitching motility and infection by the pilus-specific phage D3112cts. Mutants which lack the ability to synthesize PAI-1, PAI-2, or both autoinducers were significantly or greatly impaired in twitching motility and in susceptibility to D3112cts. Twitching motility and phage susceptibility in the autoinducer-deficient mutants were partially restored by exposure to exogenous PAI-1 and PAI-2. Both twitching motility and infection by pilus-specific phage are believed to be dependent on the extension and retraction of polar type 4 pili. Western blot analysis of whole-cell lysates and enzyme-linked immunosorbent assays of intact cells were used to measure the amounts of pilin on the cell surfaces of las and rhl mutants relative to that of the wild type. It appears that PAI-2 plays a crucial role in twitching motility and phage infection by affecting the export and assembly of surface type 4 pili. The ability of P. aeruginosa cells to adhere to human bronchial epithelial cells was also found to be dependent on the rhl quorum-sensing system. Microscopic analysis of twitching motility indicated that mutants which were unable to synthesize PAI-1 were defective in the maintenance of cellular monolayers and migrating packs of cells. Thus, PAI-1 appears to have an essential role in maintaining cell-cell spacing and associations required for effective twitching motility. (+info)
The level of expression of the minor pilin subunit, CooD, determines the number of CS1 pili assembled on the cell surface of Escherichia coli. (7/1658)CooD, the minor subunit of CS1 pili of enterotoxigenic Escherichia coli, is essential for the assembly of stable, functional pili. We previously proposed that CooD is a rate-limiting initiator of CS1 pilus assembly and predicted that the level of CooD expression should therefore determine the number of CS1 pili assembled on the cell surface. In this study, we confirm that CooD is required for the initiation of pilus assembly rather than for the stabilization of pili after they are assembled by demonstrating that specific modulation of cooD expression also modulates the number of CS1 pili on bacterial cells. (+info)
Characterization of the recD gene of Neisseria gonorrhoeae MS11 and the effect of recD inactivation on pilin variation and DNA transformation. (8/1658)Pilin antigenic variation in Neisseria gonorrhoeae may result following intrachromosomal recombination between homologous pil genes. Despite extensive study, recA is the only previously characterized gene known to be involved in this process. In this study, the gonococcal recD gene, encoding one subunit of the putative RecBCD holoenzyme, was characterized and its role in pilin variation assessed. The complete recD gene of N. gonorrhoeae MS11 was cloned and its nucleotide sequence determined. The gonococcal recD gene complemented a defined Escherichia coli recD mutant, based on plaque formation of bacteriophage lambda and the restoration of ATP-dependent nuclease activity. Inactivation of the gonococcal recD gene had no measurable effect on cell viability or survival following UV exposure, but did decrease the frequency of DNA transformation approximately threefold. The frequency at which non-parental pilin phenotypes were spawned was 12-fold greater in MS11 recD mutants compared with the parental MS11 rec+ strain. Similar results were obtained using recD mutants that were not competent for DNA transformation. Complementation of the MS11 recD mutant with a wild-type recD gene copy restored the frequency of pilin phenotypic variation to approximately wild-type levels. The nucleotide changes at pilE in the recD mutants were confined to the variable regions of the gene and were similar to changes previously attributed to gene conversion. (+info)
Identification of Novel Components Influencing Colonization Factor Antigen I Expression in Enterotoxigenic Escherichia coli -...
Identification of Novel Components Influencing Colonization Factor Antigen I Expression in Enterotoxigenic Escherichia coli. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation
In Gram-negative bacteria, type IV pilus assembly (T4PS) and type II secretion (T2SS) systems polymerize inner membrane proteins called major pilins or pseudopilins respectively, into thin filaments. Four minor pilins are required in both systems for efficient fibre assembly. Escherichia coli K-12 has a set of T4PS assembly genes that are silent under standard growth conditions. We studied the heterologous assembly of the E. coli type IV pilin PpdD by the Klebsiella oxytoca T2SS called the Pul system. PpdD pilus assembly in this context depended on the expression of the K. oxytoca minor pseudopilin genes pulHIJK or of the E. coli minor pilin genes ppdAB-ygdB-ppdC. The E. coli minor pilins restored assembly of the major pseudopilin PulG in a pulHIJK mutant, but not the secretion of the T2SS substrate pullulanase. Thus, minor pilins and minor pseudopilins are functionally interchangeable in initiating major pilin assembly, further extending the fundamental similarities between the two systems. The ...
PilMNOPQ from the Pseudomonas aeruginosa Type IV Pilus System Form a Transenvelope Protein Interaction Network That Interacts...
This is the first report to provide evidence for a complete T4P transenvelope protein interaction network formed by the products of the pilMNOPQ operon. This complex has components in the cytoplasm (PilM), inner membrane (PilN, PilO, and PilP), and outer membrane (PilQ). This arrangement of the inner and outer membrane components of the pilus assembly complex may provide an unobstructed path through the periplasm, including the peptidoglycan, for the assembled pilus. Also, hypothesized interactions of PilM with the platform protein PilC, as demonstrated for orthologs in the T2SS and the T4bP system (30-32), would connect the cytoplasmic motor with the outer membrane secretin, allowing for the efficient transmission of the signals that control its opening and closing.. Interaction of the major pilin subunit with the periplasmic components PilNOP has been reported for the N. meningitidis T4P system (23) and for corresponding components of the T2SS (23, 33, 34) and the T4bP system (35). Our ...
What is Piles? Causes, Piles Symptoms and Piles Treatments - News Web BD
There are numerous possible causes of piles. In fact, they are not nervous and not hazardous. If youre on the lookout for piles remedy or strategies to do
Ayurvedic Piles Medicine Vs Piles Surgery. Which is Better? - Pfree.in
There arent too many options if you want to root piles out completely. Perhaps in that context surgery is one of the best bets. But that too, there is no foolproof guarantee that you will never have it.
What are Some Effective Remedies for Piles? | CRH ORegan System
What are some effective remedies for piles? If youre looking for piles treatment options, youve come to the right place. Read more here.
Characterization of a class II pilin expression locus from Neisseria meningitidis: evidence for increased diversity among pilin...
Strains of Neisseria meningitidis elaborate one of two classes of pili. Meningococcal class I pili have many features in common with pili produced by N. gonorrhoeae, including the ability to bind monoclonal antibody SM1 and a common gene and protein structure consisting of conserved, semivariable, and hypervariable regions. Class II pili are SM1 nonreactive and display smaller subunit molecular weights than do gonococcal or meningococcal class I pili. In this study, we have determined the N-terminal amino acid sequence for class II pilin and isolated the expression locus encoding class II pilin from N. meningitidis FAM18. Meningococcal class II pilin displays features typical of type IV pili and shares extensive amino acid identity with the N-terminal conserved regions of other neisserial pilin proteins. However, the deduced class II pilin sequence displays several unique features compared with previously reported meningococcal class I and gonococcal pilin sequences. Class II pilin lacks several ...
Identification of Novel Components Influencing Colonization Factor Antigen I Expression in Enterotoxigenic Escherichia coli
Colonization factors (CFs) mediate early adhesion of Enterotoxigenic Escherichia coli (ETEC) in the small intestine. Environmental signals including bile, glucose, and contact with epithelial cells have previously been shown to modulate CF expression in a strain dependent manner. To identify novel components modulating CF surface expression, 20 components relevant to the intestinal environment were selected for evaluation. These included mucin, bicarbonate, norepinephrine, lincomycin, carbon sources, and cations. Effects of individual components on surface expression of the archetype CF, CFA/I, were screened using a fractional factorial Hadamard matrix incorporating 24 growth conditions. As most CFs agglutinate erythrocytes, surface expression was evaluated by mannose resistant hemagglutination. Seven components, including porcine gastric mucin, lincomycin, glutamine, and glucose were found to induce CFA/I surface expression in vitro in a minimal media while five others were inhibitory, including
Topology of the outer-membrane secretin PilQ from Neisseria meningitidis
Neisseria meningitidis is the causative agent of epidemic meningococcal meningitis and septicaemia. Type IV pili are surface organelles that mediate a variety of functions, including adhesion, twitching motility, and competence for DNA binding and uptake in transformation. The secretin PilQ is required for type IV pilus expression at the cell surface, and forms a dodecameric cage-like macromolecular complex in the meningococcal outer membrane. PilQ-null mutants are devoid of surface pili, and prevailing evidence suggests that the PilQ complex facilitates extrusion and retraction of type IV pili across the outer membrane. Defining the orientation of the meningococcal PilQ complex in the membrane is a prerequisite for understanding the structure-function relationships of this important protein in pilus biology. In order to begin to define the topology of the PilQ complex in the outer membrane, polyhistidine insertions in N- and C-terminal regions of PilQ were constructed, and their subcellular ...
Identification and validation of novel chromosomal integration and expression loci in Escherichia coli flagellar region 1.
Juhas M., Ajioka J.W.. Escherichia coli is used as a chassis for a number of Synthetic Biology applications. The lack of suitable chromosomal integration and expression loci is among the main hurdles of the E. coli engineering efforts. We identified and validated chromosomal integration and expression target sites within E. coli K12 MG1655 flagellar region 1. We analyzed five open reading frames of the flagellar region 1, flgA, flgF, flgG, flgI, and flgJ, that are well-conserved among commonly-used E. coli strains, such as MG1655, W3110, DH10B and BL21-DE3. The efficiency of the integration into the E. coli chromosome and the expression of the introduced genetic circuit at the investigated loci varied significantly. The integrations did not have a negative impact on growth; however, they completely abolished motility. From the investigated E. coli K12 MG1655 flagellar region 1, flgA and flgG are the most suitable chromosomal integration and expression loci.. PLoS One 10:e0123007-e0123007(2015) ...
Pilus assembly across the bacterial outer membrane
The chaperone-usher (CU) pathway is a translocation system used to assemble adhesive multi-subunit fibres on the outer surface of gram-negative bacteria. CU pili are formed by the non-covalent polymerisation of several hundreds or thousands of pilus subunits which consist of an incomplete immunoglobulin (Ig)-like fold lacking the C-terminal ß-strand. In the periplasm, a cognate chaperone assists in pilus subunit folding by donating a b-strand to complement the truncated Ig-like fold of the pilus subunit, a process termed donor-strand complementation (Figure 69, A*) . Chaperone:subunit complexes are then recruited to a pilus assembly platform in the outer membrane (OM) called the usher. The usher catalyses ordered subunit polymerisation and mediates translocation of the nascent pilus to the cell surface. Polymerisation of pilus subunits occurs through an intermolecular fold complementation mechanism involving the first 10-20 residues (termed N-terminal extension or Nte) of the pilus ...
Fiber assembly by the chaperone-usher pathway. | SBRC
Bacterial pathogens utilize the chaperone-usher pathway to assemble extracellular multi-subunit fibers essential for virulence. The periplasmic chaperone facilitates the initial folding of fiber subunits but then traps them in activated folding transition states. Chaperone dissociation releases the folding energy that drives subunit incorporation into the fiber, which grows through a pore formed by the outer-membrane usher.. ...
New cell surface protein involved in biofilm formation by Streptococcus parasanguinis - Fingerprint - Oregon Health &...
Fingerprint Dive into the research topics of New cell surface protein involved in biofilm formation by Streptococcus parasanguinis. Together they form a unique fingerprint. ...
Ushering Pilus Assembly: Dissecting the Mechanics of an E. coli Bacterial Nanomachine | Nature Research Microbiology Community
The paper in Nature Microbiology can be found here. The work presented in this paper was the result of a strong collaboration between the laboratories of Dr. Scott J. Hultgren and Dr. Peng Yuan at Washington University in St. Louis. Chaperone-usher pathway (CUP) pili are extracellular proteinaceous fibers ubiquitously found on Gram-negative bacteria. Type 1 and P pili are produced by uropathogenic strains of E. coli and are tipped with the FimH and PapG adhesins, respectively, to mediate host and tissue tropism to the bladder and kidney. During pilus assembly hundreds of individual pilus subunits called pilins are first exported across the inner membrane to the periplasm. Chaperone-pilin complexes are then guided to an outer membrane (OM) nanomachine called the usher, which catalyzes subunit-subunit interactions. The usher contains five functional domains: a 24-stranded transmembrane β-barrel domain, a β-sandwich plug domain that resides in the pore of the TD in the apo-usher, an ...
Streptococcus parasanguinis (strain ATCC 15912 / DSM 6778 / CIP 104372 / LMG 14537)
p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.,/p> ,p>,a href=/manual/evidences>More…,/a>,/p> ...
Effects of recA Mutations on Pilus Antigenic Variation and Phase Transitions in Neisseria gonorrhoeae | Genetics
Intragenic recombination between the single complete pilin gene (expression locus) and multiple, distinct, partial pilin gene copies (silent, storage loci) is thought to account for the generation of pilus antigenic diversity and piliation phase (on-off) changes exhibited by Neisseria gonorrhoeae. The mechanisms operating in the genomic rearrangements associated with these forms of pilus variation were investigated through the study of isogenic strains of gonococci bearing either wild-type or altered recA alleles. Examination of the rates of pilus phase variation and the genetic basis for changes in piliation status displayed by these strains show that recA mediated homologous recombination is required for these high frequency events and confirm that the nonpiliated state results from mutations in the expressed pilin gene. In a strain that is deficient in recA mediated homologous recombination, pilus phase variation occurs at a 100-1000-fold reduced rate and results predominantly from one class ...
SecA2 is distinct from SecA in immunogenic specificity, subcellular distribution and requirement for membrane anchoring in...
TY - JOUR. T1 - SecA2 is distinct from SecA in immunogenic specificity, subcellular distribution and requirement for membrane anchoring in Streptococcus parasanguis. AU - Chen, Qiang. AU - Wu, Hui. AU - Kumar, Reetu. AU - Peng, Zhixiang. AU - Fives-Taylor, Paula M.. PY - 2006/11. Y1 - 2006/11. N2 - A secA2 gene is present in the genomes of a wide variety of Gram-positive bacteria. In Streptococcus parasanguis, a primary colonizer of the tooth surface, secA2 is involved in the secretion of a small group of proteins including the fimbrial adhesin, Fap1. Although the substrate specificity is different, SecA2 is predicted to be similar to SecA in structure and function based on the homology between these two proteins. In this study, polyclonal antibodies against SecA2 and SecA did not cross-react with each other, indicating that these two proteins possessed distinct immunogenic epitopes. Fractionation analysis demonstrated that SecA2 was not evenly distributed between the cytoplasmic membrane and ...
Contribution of noncanonical antigens to virulence and adaptive immunity in human infection with enterotoxigenic E. coli |...
Enterotoxigenic E. coli (ETEC) contribute significantly to the substantial burden of infectious diarrhea among children living in low and middle income countries. In the absence of a vaccine for ETEC, children succumb to acute dehydration as well as non-diarrheal sequelae related to these infections including malnutrition. The considerable diversity of ETEC genomes has complicated canonical vaccine development approaches defined by a subset of ETEC pathovar-specific antigens known as colonization factors (CFs). To identify additional conserved immunogens unique to this pathovar we employed an open-aperture approach to capture all potential conserved ETEC surface antigens in which we mined genomic sequences of 89 ETEC isolates, bioinformatically selected potential surface-exposed pathovar-specific antigens conserved in more than 40% of the genomes (n=118), and assembled the representative proteins onto microarrays, complemented with known or putative colonization factor subunit molecules ...
Chaperone-usher fimbriae of Escherichia coli
Chaperone-usher (CU) fimbriae are adhesive surface organelles common to many Gram-negative bacteria. Escherichia coli genomes contain a large variety of characterised and putative CU fimbrial operons, however, the classification and annotation of individual loci remains problematic. Here we describe …
CAZy - GT101
Family built after PMID=25023666; The GT101 module of Streptococcus parasanguinis dGT1 catalyzes the transfer of glucose to the branch point of the hexasaccharide O-linked to the serine-rich repeat of the bacterial adhesin Fap1 ...
ASMscience | Fimbriae: Classification and Biochemistry
Proteinaceous, nonflagellar surface appendages constitute a variety of structures, including those known variably as fimbriae or pili. Constructed by distinct assembly pathways resulting in diverse morphologies, fimbriae have been described to mediate functions including adhesion, motility, and DNA transfer. As these structures can represent major diversifying elements among Escherichia and Salmonella isolates, multiple fimbrial classification schemes have been proposed and a number of mechanistic insights into fimbrial assembly and function have been made. Herein we describe the classifications and biochemistry of fimbriae assembled by the chaperone/usher, curli, and type IV pathways.
F7-2 fimbrial protein elisa and antibody
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RCSB PDB - Protein Feature View - Type IV pilus biogenesis protein PilO - Q306N4 (Q306N4 MYXXD)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Behavior of Laterally Loaded Piles: I-Single Piles
Behavior of Laterally Loaded Piles: I-Single Piles. An analysis is presented for the horizontal displacement and rotation of a vertical pile subjected to lateral loading and moment, and situated in an ideal elastic mass. Influence factors are presented for a wide range of pile flexibilities and length-to-diameter ratios, for both free-head and fixed-head piles. Comparisons between the elastic solutions and the corresponding solutions obtained from the subgrade reaction theory show that the latter considerably overestimates the displacement and rotation of the pile, but gives a reasonable estimate of the moments in the pile. The elastic analysis is extended to include the effect of local yield between the soil and the pile; the load-displacement relationship for relatively flexible piles is found to be markedly influenced by local yield. The characteristics of behavior indicated by the theoretical solutions agree reasonably well with those reported from measurements on full-scale piles.
Puzzle 92. A pile of prime-spheres
Problems & Puzzles: Puzzles Puzzle 92. A pile of prime-spheres Days ago my friend Enoch Haga and me starting puzzling each other to construct a pile of balls (a tetrahedron) with the following properties: a) every ball contains a distinct prime, b) each prime-ball must be the sum of the prime numbers contained in the three balls from the immediate inferior level and in contact with the mentioned prime-ball. Can you imagine a pile of balls? A new friend of these pages, Chuck Henry, kindly and quickly provided several beautiful photos generated by him that should help to visualize a pile of balls like the one we are talking about. Please click here 1 and here 2 (*). Question: Get the least solution for a pile of n levels, for n=5, 6 & 7. ...
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TYWKIWDBI (Tai-Wiki-Widbee): Mathematical card trick
Hi, my wife just showed me the video... watching your shuffling at the beginning you can figure out that the random part does not at all change the positions of the aces in the resulting pile. The random set of x cards is on the right pile, the remaining 15-x cards on the middle pile under the next ace. In the end the middle pile goes on the right pile making it exactly the same 15 cards between the two aces that were on the middle pile before - just randomly restacked (only those 15 cards) - the same thing happens with the other random part and the right pile. To puzzle the audience more you do the 4 cards-thing at the end ...
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Useful Tips For The Treatment of Piles. Piles are considered to be one of the common problems, a number of people are endurance from. Dealing with..
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Piles or hemorrhoids are a very common condition that most people suffer. It creates much discomfort and pain especially when going to the toilet. Learn more about piles here.
FIMA / CIMA
Fundación para la Investigación Médica Aplicada (FIMA) / Centro de Investigación Médica Aplicada (CIMA). Professor Meleros group.
Questionnaire / Movax
The MOVAX side grip pile driver is based on MOVAXs Modular System and thus capable of handling a wide variety of piles [linkki]. In order to select the correct arms, clamps and pads information is needed about the main type of piles to be driven, but also whether there is a need to drive also other type of piles, now or in the future ...
Pile It High With Things That Crunch - latimes
Its easy to make a quick stop at the deli counter, come home and pile sliced sandwich meats on a bun. But these light summer sandwiches, which have all the crunch of a salad, take little time to
KEGG BRITE: Secretion System - Ralstonia pickettii 12D
K02650 pilA; type IV pilus assembly protein PilA K02650 pilA; type IV pilus assembly protein PilA K02650 pilA; type IV pilus assembly protein PilA K02652 pilB; type IV pilus assembly protein PilB K02653 pilC; type IV pilus assembly protein PilC K02654 pilD; leader peptidase (prepilin peptidase) / N-methyltransferase [EC:22.214.171.124 2.1.1.-] K02654 pilD; leader peptidase (prepilin peptidase) / N-methyltransferase [EC:126.96.36.199 2.1.1.-] K02655 pilE; type IV pilus assembly protein PilE K02655 pilE; type IV pilus assembly protein PilE K02656 pilF; type IV pilus assembly protein PilF K02657 pilG; twitching motility two-component system response regulator PilG K02658 pilH; twitching motility two-component system response regulator PilH K02659 pilI; twitching motility protein PilI K02660 pilJ; twitching motility protein PilJ K02662 pilM; type IV pilus assembly protein PilM K02663 pilN; type IV pilus assembly protein PilN K02664 pilO; type IV pilus assembly protein PilO K02665 pilP; type IV pilus assembly ...
Post-translational processing of the major structural subunit of a type IV fimbria from enteropathogenic Escherichia coli
Localized adherence (LA) to epithelial cells is a virulence-associated phenotype of enteropathogenic Escherichia coli (EPEC), a leading cause of infantile diarrhea around the world. An inducible bundle forming pilus (BFP) was proposed to be the adhesin mediating LA. The major structural subunit of BFP (bundlin) is encoded on a large EPEC plasmid by the bfpA gene, a member of the type IV fimbria gene family. Like all type IV pilins, bundlin is synthesized as a precursor which is processed at its N-terminus into the mature form after an atypical signal peptide is cleaved, and, like most fimbrial subunits of any type, bundlin has at its C-terminus two Cysteine residues which could form a disulfide bond. The gene encoding the prepilin peptidase responsible for pre-bundlin N-terminal proteolytic processing, bfpP, was cloned from the EPEC plasmid by functional complementation of a P. aeruginosa prepilin peptidase (pilD) mutant. The predicted product of bfpP is homologous to other prepilin peptidases, ...
Colonization Factors of Enterotoxigenic Escherichia coli
View more ,Enterotoxigenic Escherichia coli (ETEC) is a major cause of life-threatening diarrheal disease around the world. The major aspects of ETEC virulence are colonization of the small intestine and the secretion of enterotoxins which elicit diarrhea. Intestinal colonization is mediated, in part, by adhesins displayed on the bacterial cell surface. As colonization of the intestine is the critical first step in the establishment of an infection, it represents a potential point of intervention for the prevention of infections. Therefore, colonization factors (CFs) have been important subjects of research in the field of ETEC virulence. Research in this field has revealed that ETEC possesses a large array of serologically distinct CFs that differ in composition, structure, and function. Most ETEC CFs are pili (fimbriae) or related fibrous structures, while other adhesins are simple outer membrane proteins lacking any macromolecular structure. This chapter reviews the genetics, structure, ...
Identification of bacterial factors involved in type 1 fimbria expression using an Escherichia coli K12 proteome chip<...
TY - JOUR. T1 - Identification of bacterial factors involved in type 1 fimbria expression using an Escherichia coli K12 proteome chip. AU - Chen, Yi Wen. AU - Teng, Ching Hao. AU - Ho, Yu Hsuan. AU - Ho, Tien Yu Jessica. AU - Huang, Wen Chun. AU - Hashimoto, Masayuki. AU - Chiang, I. Yuan. AU - Chen, Chien Sheng. PY - 2014/6. Y1 - 2014/6. N2 - Type 1 fimbriae are filamentous structures on Escherichia coli. These structures are important adherence factors. Because binding to the host cells is the first step of infection, type 1 fimbria is an important virulence factor of pathogenic E. coli. Expression of type 1 fimbria is regulated by a phase variation in which each individual bacterium can alternate between fimbriated (phase-ON) and nonfimbriated (phase-OFF) states. The phase variation is regulated by the flipping of the 314-bp fimS fragment, which contains the promoter driving the expression of the genes required for the synthesis of type 1 fimbria. Thus, the bacterial proteins able to interact ...
Genetic Diversity of the Gene Cluster Encoding Longus, a Type IV Pilus of Enterotoxigenic Escherichia coli | Journal of...
Within-group analysis of lngA sequences identified three distinct allelic variants within group 1 and three variants within group 2 that differed from each other by three to seven nucleotides (Fig. 2a). However, all 15 within-group mutations resulted in amino acid substitutions, indicating that these point replacements accumulated under strong positive selection. While immune escape may drive the accumulation of these point mutations, the changes may also be functionally adaptive for ETEC isolates by modifying some functional (e.g., putative adhesive) properties of Longus fimbriae, as shown for mannose- and di-galactose-specific fimbrial adhesins of uropathogenic E. coli (24, 25, 32), bfpA of EPEC (3), decay-accelerating factor-specific Dr adhesins of uropathogenic and diarrheal E. coli (6, 17, 31), and type 1 fimbrial adhesin of Salmonella enterica serovar Typhimurium (4).. Alignment of predicted LngA protein sequences (182 amino acids long) revealed that the between-group diversity of the ...
Volume 65, Issue 2 | The American Journal of Tropical Medicine and Hygiene
Infection caused by enterotoxigenic Escherichia coli (ETEC) poses a serious health problem among children and adults in developing countries. Colonization of the small intestinal mucosa by ETEC strains is mediated by antigenically specific fimbriae, also known as colonization factor antigens (CFA). The significance of this study arises from reports that active and passive immunization with ETEC strains harboring CFAs has previously been shown to induce protective immunity against diarrhea in animal models. The aim of this study was to determine toxin-associated CFAs of ETEC isolated from a diarrheal disease case-control study in Jakarta, Indonesia. Thirteen hundred and twenty-three diarrheic and control patients with lactose-fermenting colonies were screened by ganglioside GM1-enzyme-linked immunosorbent assay (GM1-ELISA) for heat-labile (LT) and heat-stable (ST) toxins. Two hundred and forty-six (19%) ETEC isolates identified by GM1-ELISA for the LT/ST toxins were screened for CFAs by Dot blot ...
ASMscience | Neisseria gonorrhoeae: A
Uncomplicated gonorrhea is most commonly an acute urogenital infection involving the urethra in men and the endocervix in women of reproductive age. Upon entering the urogenital tract, Neisseria gonorrhoeae adheres to columnar epithelial cells, a step that presumably enables the gonococcus to withstand the flushing force of urine and the constant shedding of cervical mucus. Transcriptional regulation of genes in response to environmental stimuli in the mucus layer may coordinate adaptation of this pathogen to nutritionally different microenvironments and to physiological stress induced by nonspecific host defenses. The major pilin subunit, PilE, is encoded by one or two expression loci (pilE) on the chromosome. This chapter focuses on the survival mechanisms utilized by the gonococcus specifically in the context of the urogenital mucosa. The isolation of a gonococcal fur mutant by Thomas and Sparling facilitated the identification of numerous fur-dependent iron-induced (Fip) and iron-repressed (Frp)
Factors intervening in the variations of in vitro adhesion power of enterotoxinogenic colibacillus (ETEC) to human enterocytes]...
Human enterotoxigenic Escherichia coli adhere to the brush border of human enterocytes. The mean number of bacteria adhering to one enterocyte (adhesion index) varied from 0.5 to 3.1 when the strains produce adhesins. Different factors related to enterocytes and to bacteria are involved in this variability. The number of bacteria which adhered to enterocytes issued from the same donor varied from from 0 to 12. Moreover the proportion of enterocytes on which several bacteria sticked did not exceed 20%. This variability might be due to the disparity in the maturation of the enterocytes. On the other hand, whatever the adhesion factors considered, the adhesion index varied according to the donors. ETEC strains did not express adhesion when bacteria were grown in a liquid medium but this capacity could be restored after transfer on solid medium. This phenomenon seemed like a phase-variation and appeared to be linked to a 4 to 6 kilobases (kb) plasmid. On the other hand, when the bacteria were grown on agar
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IUCr) A slow-forming isopeptide bond in the structure of the major pilin SpaD from Corynebacterium diphtheriae has implications...
The essential lysine, Lys179 in SpaD, is in the floor of the groove and is mostly covered by the mobile β1-β2 loop. A similar β1-β2 loop is present in all major pilins for which full-length structures are available, except for Spy0128, which lacks an equivalent YPKN pilin motif. This β1-β2 loop flanks a similar groove in each case, but is usually disordered. In SpaD it is disordered in one molecule and ordered but with high B factors in the other. This flexibility may have a role in pilus polymerization, with the loop preventing unwanted inter-actions by covering the groove and then opening up to allow binding of the sortase-recognition segment of another molecule.. Our results showing a mixture of SpaD species, with the D1 internal isopeptide bond either formed or not formed, indicate that the bond in the N domain may not be fully formed in a SpaD monomer. An energy barrier clearly exists, possibly conformational in nature as shown for RrgB, and this can be overcome in vitro by warming. ...
RCSB PDB - 3HTL: Structure of the Corynebacterium diphtheriae major pilin SpaA points to a modular pilus assembly with...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
This protein is required for the assembly of the type IV fimbria in Pseudomonas aeruginosa responsible for twitching motility, and for a similar pilus-like structure in Synechocystis. It is also found in species such as Deinococcus described as having natural transformation (for which a type IV pilus-like structure is proposed) but not fimbria ...
Fimbriae Function, Definition & Anatomy | Body Maps
The fimbriae of the uterine tube, also known as fimbriae tubae, are small, fingerlike projections at the end of the fallopian tubes, through which eggs move from the ovaries to the uterus. The fimbriae are connected to the ovary.
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LSHTM Research Online
Forslund, AL; Kuoppa, K; Svensson, K; Salomonsson, E; Johansson, A; Bystrom, M; Oyston, PCF; Michell, SL; Titball, RW; Noppa, L; +3 more... Frithz-Lindsten, E; Forsman, M; Forsberg, A; (2006) Direct repeat-mediated deletion of a type IV pilin gene results in major virulence attenuation of Francisella tularensis. Molecular microbiology, 59 (6). pp. 1818-1830. ISSN 0950-382X DOI: https://doi.org/10.1111/j.1365-2958.2006.05061.x Full text not available from this repository ...
Getting My medical and Health news To Work
Genetic transformation is the method by which a receiver bacterial cell usually takes up DNA from a neighboring mobile and integrates this DNA to the receivers genome by recombination. In N. meningitidis, DNA transformation involves the existence of short DNA sequences (9-10 mers residing in coding areas) on the donor DNA. These sequences are termed DNA uptake sequences (DUSs). Specific recognition of these sequences is mediated by a type IV pilin ...
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bacteria fimD protein Summary Report | CureHunter
bacteria fimD protein: fimD gene necessary for surface localization of type 1 fimbriae; amino acid sequence given in first source
Get Rid of Bleeding Piles Within 30 Minutes Permanently - 2020 Guide | Boomloaded
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Publications | Experimental Soft Condensed Matter Group
AbstractNeisseria gonorrheae bacteria are the causative agent of the second most common sexually transmitted infection in the world. The bacteria move on a surface by means of twitching motility. Their movement is mediated by multiple long and flexible filaments, called type IV pili, that extend from the cell body, attach to the surface, and retract, thus generating a pulling force. Moving cells also use pili to aggregate and form microcolonies. However, the mechanism by which the pili surrounding the cell body work together to propel bacteria remains unclear. Understanding this process will help describe the motility of N. gonorrheae bacteria, and thus the dissemination of the disease which they cause. In this article we track individual twitching cells and observe that their trajectories consist of alternating moving and pausing intervals, while the cell body is preferably oriented with its wide side toward the direction of motion. Based on these data, we propose a model for the collective ...
Patent US4818148 - Frost damage proofed pile - Google Patents
A covering is applied onto the outer surface of a pile including a steel pipe or the like to surround a predetermined length thereof so as to reduce a frost heaving force or negative friction acting on the pile in a frigid area. The covering is closely adhered by an adhesion layer the like to the pile over a given length thereof. This given length is between 0.5 and 5 m. The covering member includes a smooth-surfaced plastic covering or elastic covering. A rugged surface covering may be provided below the smooth surfaced covering.
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Piles | Symptoms & Treatments | Spire Healthcare
Piles are swellings that develop inside your back passage (rectum) or around your bottom (anus) which can cause bleeding and pain. Piles are also known as haemorrhoids. Find out more about the condition and how Spire Healthcare can treat piles.
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Raios dorsais (total): 9; Vértebras: 35 - 37. Vomero-palatine organ with only 5 pairs of fimbriate lamella; lamellae comparatively broad, each with up to 20 short fimbriae. Body dull white or silvery overall; fins dusky; colorless; humeral mark present, otherwise no distinctive markings. ...
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M rgised taimed
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Rgp gingipains are necessary for adhesion and invasion as they processed precursor proteins of long fimbriae. The P. gingivalis ... Short fimbriae (Mfa1), also known as minor fimbriae, have distinct roles from long fimbriae and are characterized to be ... P. gingivalis has long fimbriae, short fimbriae, and accessory components, each of which have distinct functions. Long fimbriae ... Fim C, D, and E accessory components associate with the main FimA protein and have a role in binding with matrix proteins and ...
The genes encoding for many of these proteins, such as fhaB (filamentous hemagglutinin) and fimA (fimbriae), as well as genes ... Components enabling its attachment include pili, filamentous hemagglutinin, fimbriae, protein autotransporters, outer membrane ... and transferrin-binding proteins. The typical host for Bordetella avium is the turkey. In turkeys this bacteria primarily leads ... which are then attached to proteins that are critical for the cell's survival. Virulence also requires iron, which is acquired ...
Bacterial cell structure
Fimbriae (sometimes called "attachment pili") are protein tubes that extend out from the outer membrane in many members of the ... Fimbriae usually function to facilitate the attachment of a bacterium to a surface (e.g. to form a biofilm) or to other cells ( ... About half of the dry mass of a bacterial cell consists of carbon, and also about half of it can be attributed to proteins. ... The periplasm is thought to exist in a gel-like state rather than a liquid due to the high concentration of proteins and ...
Fimbriae are formed of a protein called pilin (antigenic) and are responsible for the attachment of bacteria to specific ... A fimbria (plural fimbriae also known as a pilus, plural pili) is a short, thin, hair-like filament found on the surface of ... The subunit protein of microfilaments is a small, monomeric protein called actin. The subunit of microtubules is a dimeric ... The ER has two forms: the rough ER, which has ribosomes on its surface that secrete proteins into the ER, and the smooth ER, ...
Most fimbria of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the ... However, as in the case of the CFA1 fimbriae, the structural protein itself can sometimes act as an adhesion if a portion of ... Davies, J. K.; Koomey, J. M.; Seifert, H. S. (1994). "Pili (fimbriae) of Neisseria gonorrhoeae". In Klemm, Per (ed.). Fimbriae ... The bacterium synthesizes a precursor protein consisting of 300 amino acids then processes the protein by removing several ...
... is capable of carrying out its normal functions of regulating curli proteins (fimbria) and producing ECM polysaccharides ( ... D59 of the protein's primary sequence. Therefore, in the protein's active form (unphosphorylated), ... CsgD is a transcription and response regulator protein referenced to as the master modulator of bacterial biofilm development. ... encoding for curli fimbriae, and adrA, encoding for the synthesis of cyclic diguanylate. In this context, c-di-GMP functions as ...
There are many fimbriae on mainly Gram-negative bacteria, so displaying proteins on fimbriae is advantageous over some other ... There are various scaffolds which have been used such as outer membrane proteins, fimbriae/flagella proteins and CPX ( ... Once the heterologous protein has been fused with the bacterial cell surface protein, it is exposed to either an enzyme, a cell ... Another method of heterologous protein fusion is fusion with fimbriae/flagella, which are filamentous protrusions on the cell ...
Fimbrial usher protein
The fimbrial usher protein is involved in biogenesis of the pilus in Gram-negative bacteria. The biogenesis of some fimbriae ( ... Schifferli DM, Alrutz MA (1994). "Permissive linker insertion sites in the outer membrane protein of 987P fimbriae of ... The usher protein has a molecular weight ranging from 86 to 100 kDa and is composed of a membrane-spanning 24-stranded beta ... Capitani G, Eidam O, Grütter MG (2006). "Evidence for a novel domain of bacterial outer membrane ushers". Proteins. 65 (4): 816 ...
The fimbriae is assembled by a chaperone-usher system, and proteins required for the assembly are expressed by the Pap operon, ... The main body of the fimbriae is composed of approx. 1000 copies of the major fimbrial subunit protein PapA, forming a helical ... P fimbriae mediate adherence to host cells, a key event in the pathogenesis of urinary tract infections. P fimbriae are large, ... June 2019). "Fimbriae reprogram host gene expression - Divergent effects of P and type 1 fimbriae". PLOS Pathogens. 15 (6): ...
P. gingivalis mutants lacking OmpA-like proteins Pgm6/7 had reduced adherence to stimulated HUVECs, but fimbriae-deficient ... Shirure VS, Reynolds NM, Burdick MM (2012). "Mac-2 binding protein is a novel E-selectin ligand expressed by breast cancer ... E-selectin recognizes and binds to sialylated carbohydrates present on the surface proteins of certain leukocytes. E-selectin ...
Fimbriae (sometimes called "attachment pili") are fine filaments of protein, usually 2-10 nanometres in diameter and up to ... The bacterial flagellum is made of about 20 proteins, with approximately another 30 proteins required for its regulation and ... Fimbriae are believed to be involved in attachment to solid surfaces or to other cells, and are essential for the virulence of ... Flagella are rigid protein structures, about 20 nanometres in diameter and up to 20 micrometres in length, that are used for ...
Fimbriae+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH). ... All fimbriae are pili; they are only called fimbriae because of their purpose. The term "fimbria" can refer to many different ( ... including type 1 fimbriae and the P fimbriae. Fimbriae are one of the primary mechanisms of virulence for E. coli, Bordetella ... Fimbriae are only visible with the use of an electron microscope. They may be straight or flexible. Fimbriae possess adhesins ...
... by binding their interfacing regions and facilitating correct protein folding before bringing them to the usher/fimbriae ... In chaperone/usher fimbriae there are broadly two types of classification: Based on the type of usher present, or based on the ... These fimbriae are built up out of modular pilus subunits, which are transported into the periplasm in a Sec dependent manner. ... Chaperone/usher fimbriae have many roles, in many stages of pathogenicity in several species. The most well understood are ...
The P fimbriae island contains virulence factors such as haemolysin, pili, cytotoxic necrosing factor, and uropathogenic ... Regulation genes typically encoded on PAIs include AraC-like proteins and two-component response regulators. PAIs can be ... 1990). "Deletions of chro- mosomal regions coding for fimbriae and hemolysins occur in vivo and in vitro in various ... specific protein (USP). Yersinia pestis high pathogenicity island I has genes regulating iron uptake and storage. Salmonella ...
Fimbriae are formed of a protein called pilin (antigenic) and are responsible for the attachment of bacteria to specific ... Fimbriae. A fimbria (plural fimbriae also known as a pilus, plural pili) is a short, thin, hair-like filament found on the ... Protein synthesis. Main article: Protein biosynthesis. Cells are capable of synthesizing new proteins, which are essential for ... linear molecules (chromosomes) with histone proteins RNA/protein synthesis coupled in the cytoplasm RNA synthesis in the ...
A-type linkages may be important for the ability of PACs to bind to proteins, such as the adhesins present on E. coli fimbriae ...
Binding of this protein to the consensus sequence represents gene expression by reducing transcription. It is not known what ... These include filamentous haemaglutinin, pertactin, fimbriae, and pertussis toxin (though expression of pertussis toxin is ... the so-called Bvg-activated repressor protein, BvgR. BvgR binds to a consensus sequence present within the coding sequences of ... and this interaction with epithelial cells is mediated by a series of protein adhesins. ...
Other adhesins are fimbriae and petractin. Once anchored, the bacterium produces tracheal cytotoxin, which stops the cilia from ... The bacterium contains a surface protein, filamentous haemagglutinin adhesin, which binds to the sulfatides found on cilia of ... The toxin, known as pertussis toxin (or PTx), inhibits G protein coupling that regulates an adenylate cyclase-mediated ... They are being replaced by acellular vaccines, composed of purified surface antigens, mainly fimbriae, filamentous ...
Immunoglobulin-binding protein - Proteins such as protein A, protein G, and protein L that are capable of binding to antibodies ... This includes parts (coats, capsules, cell walls, flagella, fimbriae, and toxins) of bacteria, viruses, and other ... An autoantigen is usually a self-protein or protein complex (and sometimes DNA or RNA) that is recognized by the immune system ... In order to induce an immune response, it needs to be attached to a large carrier molecule such as a protein (a complex of ...
Proteins with receptor sites are located on the plasma membrane, binding to specific solutes. The receptor proteins that are ... Two unique characteristics of prokaryotes are fimbriae (finger-like projections on the surface of a cell) and flagella ( ... The pathway for proteins to move in cells starts at the ER. Lipids and proteins are synthesized[clarification needed] in the ER ... Receptor proteins are recycled back to the plasma membrane by the same vesicle. Betts, J. Gordon; et al. (April 25, 2013). "3.5 ...
Using molecular markers such as green fluorescent protein or fluorescent antibodies, it is possible to monitor the location of ... These include motility, chemotaxis to seed and root exudates, production of pili or fimbriae, production of specific cell ... The rhizobacteria require oxygen to metabolize, so oxygen is provided by a hemoglobin protein called leghemoglobin which is ... "Simultaneous imaging of Pseudomonas fluorescens WCS365 populations expressing three different autofluorescent proteins in the ...
The terms pilus and fimbria (Latin for 'fringe'; plural: fimbriae) can be used interchangeably, although some researchers ... All pili in the latter sense are primarily composed of pilin proteins, which are oligomeric. Dozens of these structures can ... Fimbriae are either located at the poles of a cell or are evenly spread over its entire surface. Mutant bacteria that lack ... This family was originally identified as "type IV fimbriae" by their appearance under the microscope. This classification ...
Two encode proteins involved in pathogenicity. The third island only codes for hypothetical proteins. They also found more ... fimbriae mediate attachment of the bacterium to the epithelial cells of the nasopharynx. It infects the cell by sticking to it ... Factor H binding protein (fHbp) that is exhibited in N. meningitidis and some commensal species is the main inhibitor of the ... Factor H binding protein is key to the pathogenesis of N. meningitidis, and is, therefore, important as a potential vaccine ...
Functional amyloid in Homo sapiens: Intralumenal domain of melanocyte protein PMEL Peptide/protein hormones stored as amyloids ... Amyloids may also have normal biological functions; for example, in the formation of fimbriae in some genera of bacteria, ... protein receptors, soluble proteins, RNAs, small metabolites, etc. In the clinical setting, amyloid diseases are typically ... One amyloid protein is infectious and is called prion in which the infectious form can act as a template to convert other non- ...
... pili or fimbriae (also fimbria, from the Latin word for "fringe") can be trapped by Tamm-Horsfall protein via its mannose- ... When this protein is concentrated at low pH, it forms a gel. Uromodulin represents the most abundant protein in normal human ... Tamm-Horsfall protein)--the most abundant protein in mammalian urine". Biochimica et Biophysica Acta (BBA) - Gene Structure and ... mainly consisting of immunoglobulin light chain known as Bence Jones protein, but often also contain Tamm-Horsfall protein. ...
Using green fluorescent protein (GFP) and E. coli strains, the bacteria are seen to gather specifically around the opercula, ... The bacteria contain type 1 fimbriae, which possess adhesin FimH at their fimbrial tips. FimH interacts with a mannosylated ...
Type IV pilin proteins are α+β proteins characterized by a very long N-terminal alpha helix. The assembly of these pili relies ... Although not all bacteria have pili or fimbriae, bacterial pathogens often use their fimbriae to attach to host cells. In Gram- ... These bonds provide enhanced mechanical and proteolytic stability to the pilin protein. Recently, the pilin protein from ... negative bacteria, where pili are more common, individual pilin molecules are linked by noncovalent protein-protein ...
In euglenids, it is formed from protein strips arranged spirally along the length of the body. Familiar examples of protists ... In some protozoa, the pellicle hosts epibiotic bacteria that adhere to the surface by their fimbriae (attachment pili). Some ...
Fimbriae Proteins | Journal of Bacteriology
The Pneumococcal Type 1 Pilus Genes Are Thermoregulated and Are Repressed by a Member of the Snf2 Protein Family Alan Basset, ... Antibodies Damage the Resilience of Fimbriae, Causing Them To Be Stiff and Tangled Bhupender Singh, Narges Mortezaei, Stephen J ... Glycosylation of Pilin and Nonpilin Protein Constructs by Pseudomonas aeruginosa 1244 Mohammed Qutyan, Matthew Henkel, Joseph ... Significance of a Posttranslational Modification of the PilA Protein of Geobacter sulfurreducens for Surface Attachment, ...
MULTISPECIES: type 1 fimbria regulatory protein FimE [Proteobacteria] - Protein - NCBI
MULTISPECIES: type 1 fimbria regulatory protein FimE [Proteobacteria] MULTISPECIES: type 1 fimbria regulatory protein FimE [ ... MULTISPECIES: type 1 fimbria regulatory protein FimE [Proteobacteria]. NCBI Reference Sequence: WP_000044711.1 ... Transcript/Protein Information [PANTHER Classification System] Transcript/Protein Information. PANTHER Classification System ... The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in ...
RCSB PDB - Protein Feature View - Fimbria adhesin protein - P21648 (MRKD KLEPN)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... The Protein Feature View requires a browser that supports SVG (Scalable Vector Graphics). Mouse over tracks and labels for more ... Data in red indicates combined ranges of Homology Models from SBKB and the Protein Model Portal ...
RCSB PDB - Protein Feature View - Fimbria adhesin EcpD - Q8FKL3 (ECPD ECOL6)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... This protein in other organisms (by gene name): Q8FKL3 - Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) 1 * B7UJD8 ... Protein disorder predictions are based on JRONN (Troshin, P. and Barton, G. J. unpublished), a Java implementation of RONN * ... The Protein Feature View requires a browser that supports SVG (Scalable Vector Graphics). Mouse over tracks and labels for more ...
fimB - Type 1 fimbriae regulatory protein FimB - Escherichia coli (strain K12) - fimB gene & protein
These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene ... FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. ... FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate ... Type 1 fimbriae regulatory protein FimBAdd BLAST. 200. Proteomic databases. jPOST - Japan Proteome Standard Repository/Database ...
Dominant low responsiveness in the IgG response of mice to the complex protein antigen type 1 fimbriae from Actinomyces...
Dominant low responsiveness in the IgG response of mice to the complex protein antigen type 1 fimbriae from Actinomyces ... Dominant low responsiveness in the IgG response of mice to the complex protein antigen type 1 fimbriae from Actinomyces ... Dominant low responsiveness in the IgG response of mice to the complex protein antigen type 1 fimbriae from Actinomyces ... Dominant low responsiveness in the IgG response of mice to the complex protein antigen type 1 fimbriae from Actinomyces ...
Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection.
This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)-cholera toxin B ... This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)-cholera toxin B ... The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and ...
Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae. |...
Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae.. ... It can be concluded that 35-day-old piglet mucus contains both protein and glycolipid receptors specific for K88 fimbriae, the ... Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae. ... Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae. ...
Structural and adhesive properties of the long polar fimbriae protein LpfD from adherent-invasive Escherichia coli
... Fanny ... "Structural and Adhesive Properties of the Long Polar Fimbriae Protein LpfD from Adherent-invasive Escherichia Coli." Acta ... "Structural and Adhesive Properties of the Long Polar Fimbriae Protein LpfD from Adherent-invasive Escherichia Coli." ACTA ... 2015). Structural and adhesive properties of the long polar fimbriae protein LpfD from adherent-invasive Escherichia coli. ACTA ...
Fimbriae Y protein elisa and antibody
Recombinant Protein and Fimbriae Y protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are ... Fimbriae Y protein ELISA Kit. Fimbriae Y protein Recombinant. Fimbriae Y protein Antibody. ... Protein Family Fimbriae Y protein. LOG IN MY ACCOUNT CART CONTENTS CHECKOUT ... Rec./Purified Protein. Custom ELISA Kit. Custom Protein. Custom Antibody. Antibody Matched Pairs. Antibody Peptide Pairs. ...
A new type of glycoconjugate vaccine containing Klebsiella fimbriae type 1 and 3 as carrier proteins | Critical Care | Full Text
A new type of glycoconjugate vaccine containing Klebsiellafimbriae type 1 and 3 as carrier proteins. *D Witkowska1. , ... Fimbriae mediate the attachment of many pathogenic bacteria to host cells. The mannose-specific fimbriae of type 1 are ... We have also undertaken the studv on stimulation of cytokine induction by fimbriae. The type 3 of Klebsiella fimbriae are ... A new type of glycoconjugate vaccine containing Klebsiellafimbriae type 1 and 3 as carrier proteins. Crit Care 1, P034 (1997). ...
Inhibition of S-fimbria-mediated adhesion to human ileostomy glycoproteins by a protein isolated from bovine colostrum. |...
Inhibition of S-fimbria-mediated adhesion to human ileostomy glycoproteins by a protein isolated from bovine colostrum.. AC ... The protein appears to block receptor sites for S- fimbriae on ileostomy glycoproteins, with steric hindrance being the most ... Inhibition of S-fimbria-mediated adhesion to human ileostomy glycoproteins by a protein isolated from bovine colostrum. ... Analysis of the amino acid sequence of the amino terminus of the 18K protein showed similarity with the sequence of beta- ...
Microbiology Society Journals | Inner-membrane protein MorC is involved in fimbriae production and biofilm formation in...
There was, however, a reduction in the secretion of fimbrial subunits, and fewer fimbriae were observed on the surface of the ... To investigate further the relationship between MorC and fimbriation, we identified and complemented the defect in fimbriae ... study employing an afimbriated strain and a developed mutant isogenic for the inner-membrane protein morphogenesis protein C ( ... MorC) revealed that the abundance of the proteins of the fimbrial secretion apparatus in the membrane is dependent on MorC. ...
The role of SEF14 and SEF17 fimbriae in the adherence of Salmonella enterica serotype Enteritidis to inanimate surfaces
To gain an understanding of the role of fimbriae and flagella in the adherence of Salmonella enterica serotype Enteritidis to ... Bacterial Proteins / physiology * Fimbriae Proteins* * Fimbriae, Bacterial / genetics * Fimbriae, Bacterial / immunology * ... The role of SEF14 and SEF17 fimbriae in the adherence of Salmonella enterica serotype Enteritidis to inanimate surfaces J Med ... To gain an understanding of the role of fimbriae and flagella in the adherence of Salmonella enterica serotype Enteritidis to ...
Purification and characterization of type 1 fimbriae of Salmonella typhi
Purified fimbriae retained their ability to bind to erythrocytes in a mannose-inhibitable fashion and, in doing so, behaved ... Type 1 fimbriae have been purified from a Salmonella typhi strain of clinical origin. ... of the purified fimbrial preparation using an antiserum raised against native fimbriae suggested that fimbrial proteins did not ... Type 1 fimbriae have been purified from a Salmonella typhi strain of clinical origin. Purified fimbriae retained their ability ...
45 kDa Fimbria Protein of Proteus mirabilis as Hemagglutinin and Adhesion Protein
... Mufida, Diana Chusna; Suswati, Enny; Wahyudi ... 45 kDa Fimbria Protein of Proteus mirabilis as Hemagglutinin and Adhesion Protein. DSpace/Manakin Repository. Login ... The 45 kDa fimbriae protein was also adhesion protein showed by its activity to adherence to rabbit vesica urinaria epithel ... The study showed that the 45 kDa fimbria protein of P mirabilis was a hemagglutinin protein that could agglutinate mice ...
Structural and adhesive properties of the long polar fimbriae protein LpfD from adherent-invasive Escherichia coli. | SBRC
Escherichia coli Proteins, Fimbriae Proteins, Humans, Intestine, Small, Mice, Models, Molecular, Molecular Sequence Data, ... Structural and adhesive properties of the long polar fimbriae protein LpfD from adherent-invasive Escherichia coli.. Title. ... Structural and adhesive properties of the long polar fimbriae protein LpfD from adherent-invasive Escherichia coli.. ... AIEC strains express long polar fimbriae (LPF), which are known to target Peyers patches in a mouse CD model. Here, the ...
Solution NMR structure of the plasmid-encoded fimbriae regulatory protein PefI from Salmonella enterica serovar Typhimurium<...
Solution NMR structure of the plasmid-encoded fimbriae regulatory protein PefI from Salmonella enterica serovar Typhimurium. ... Solution NMR structure of the plasmid-encoded fimbriae regulatory protein PefI from Salmonella enterica serovar Typhimurium. / ... Solution NMR structure of the plasmid-encoded fimbriae regulatory protein PefI from Salmonella enterica serovar Typhimurium. ... title = "Solution NMR structure of the plasmid-encoded fimbriae regulatory protein PefI from Salmonella enterica serovar ...
Long polar fimbriae of enterohemorrhagic Escherichia coli O157: H7 bind to extracellular matrix proteins<...
Long polar fimbriae of enterohemorrhagic Escherichia coli O157: H7 bind to extracellular matrix proteins. Infection and ... Long polar fimbriae of enterohemorrhagic Escherichia coli O157 : H7 bind to extracellular matrix proteins. In: Infection and ... Long polar fimbriae of enterohemorrhagic Escherichia coli O157 : H7 bind to extracellular matrix proteins. / Farfan, Mauricio J ... title = "Long polar fimbriae of enterohemorrhagic Escherichia coli O157: H7 bind to extracellular matrix proteins", ...
Sequence analyses of fimbriae subunit FimA proteins on Actinomyces naeslundii genospecies 1 and 2 and Actinomyces odontolyticus...
... assembly and sorting of fimbrial proteins. The highly conserved pilin, E box and LPXTG motifs are present in fimbriae proteins ... All three FimA protein variants contained a signal peptide, pilin motif, E box, proline-rich segment and an LPXTG sorting motif ... Western blot analyses with FimA antisera revealed monomers and oligomers of FimA in whole cell protein extracts and a purified ... The genus Actinomyces involves a diversity of unique FimA proteins with conserved pilin, E box and LPXTG motifs, depending on ...
Efficacy of bacterin-, outer membrane protein- and fimbriae extract-based vaccines for the control of Salmonella Enteritidis...
Animals , Bacterial Outer Membrane Proteins , Fimbriae, Bacterial , Chickens/immunology , Bacterial Vaccines/therapeutic use , ... Efficacy of bacterin-, outer membrane protein- and fimbriae extract-based vaccines for the control of Salmonella Enteritidis ... Efficacy of bacterin-, outer membrane protein- and fimbriae extract-based vaccines for the ... outer membrane proteins (OMP) and fimbriae crude extract (FE). The chickens were vaccinated intramuscularly with two doses of ...
Articles of Significant Interest in This Issue | Applied and Environmental Microbiology
Streptococcus suis Fimbria-Like Protein SssP1 Contributes to Bacterial Virulence. Streptococcus suis is an important zoonotic ... e01385-18) demonstrated that SssP1, a novel fimbria-like protein transported by the SecY2/A2 system, contributes to the ... The mechanisms by which S. suis constructs these fimbria-like structures on the cell surface and adheres to host cells were ... Chemical activity-based probes facilitate identification and measurement of protein activity from any biological sample. Rosnow ...
Amyloid-β induces synaptic dysfunction through G protein-gated inwardly rectifying potassium channels in the fimbria-CA3...
Amyloid-β induces synaptic dysfunction through G protein-gated inwardly rectifying potassium channels in the fimbria-CA3 ... Here, the acute effects of Aβ on CA3 pyramidal neurons and their synaptic activation from septal part of the fimbria were ... In addition, it was found that the specific pharmacological modulation of the GABAB receptor effector, G-protein-coupled inward ... system where learning and memory processes take place depending on accurate oscillatory activity tuned at fimbria-CA3 synapse. ...
Reconstruction of Long Noncircumferential Tracheal or Carinal Resections with Bronchial Flaps.
McGrath videolaryngoscope versus Macintosh laryngoscope for tracheal intubation: A systematic review and meta-analysis with...
Fimbria (bacteriology) - Wikipedia
Fimbriae+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH). ... All fimbriae are pili; they are only called fimbriae because of their purpose. The term "fimbria" can refer to many different ( ... including type 1 fimbriae and the P fimbriae. Fimbriae are one of the primary mechanisms of virulence for E. coli, Bordetella ... Fimbriae are only visible with the use of an electron microscope. They may be straight or flexible. Fimbriae possess adhesins ...
Common components in the assembly of type 4 fimbriae, DNA transfe...: Ingenta Connect
These may either be true type 4 fimbriae, or components of protein complexes which act in the transport of macromolecules (DNA ... Common components in the assembly of type 4 fimbriae, DNA transfer systems, filamentous phage and protein‐secretion apparatus: ... Pil‐like proteins are also involved in protein secretion, DNA transfer by conjugation and transformation, and morphogenesis of ... The Pseudomonas aeruginosa genes pilB‐D and pilQ are necessary for the assembly of type 4 fimbriae. Homologues of these genes ...
Rapid detection of food-borne pathogens by using molecular techniques.
Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae: effects of insert position and host background. -...
The potential of the major structural protein of type 1 fimbriae as a display system for heterologous sequences was tested. As ... Display of chimeric fimbriae was tested with respect to host background in three different Escherichia coli strains, i.e. an ... Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae: effects of insert position and host background.. ... Several of the chosen positions seemed amenable even for large foreign inserts; the chimeric proteins were exposed on the ...
Free Science Flashcards about Microbiology
AdhesinsAdhesionPiliOuter membrane proteinSubunitColiBacteriaAntigensStrainsAntigenProline-rich proteinsSalmonellaReceptorsBiofilmAntibodiesExpression of type 1 fimbriaeReceptorAutotransporterGenePertactinColonizationAdherenceBindStructuresGlycoproteinsPilusSecretionGenesActinomycesVaccinesStrainChimeric fimbriaePorphyromonasExtracellularSerumVitroSalivaryMembraneBiosynthesisElectrophoresisRegulatoryIsolateProteinaceous fibrilsInfectionFilamentousSubunitsAntibodyBacterial surfaceTypeToxin
- Fimbriae possess adhesins which attach them to some sort of substratum so that the bacteria can withstand shear forces and obtain nutrients. (wikipedia.org)
- Certain fimbriae are protein adhesins. (vetstream.com)
- Although recent studies suggest that amyloid adhesins are abundant in natural biofilms ( 22 ), curli fimbriae remain the sole example of an amyloid protein that has been shown to be an important functional component of a biofilm matrix. (pnas.org)
- It is hypothesized that a mucosal vaccine against a combination of S. mutans surface proteins would protect against dental caries by inducing specific salivary immunoglobulin A (IgA) antibodies which may reduce bacterial pathogenesis and adhesion to the tooth surface by affecting several adhesins simultaneously. (asm.org)
- Fimbriae are surface appendages assembled from protein subunits, with distal adhesins specifically recognizing host-cell receptors. (diva-portal.org)
- Major variable surface components include pili (fimbriae), the pilus-associated adhesin PilC, the opacity protein (Opa) family of bacterial adhesins, and LPS. (rupress.org)
- We have shown that 2 adhesins of this organism, the major and minor fimbriae proteins bind to and signal through TLR2 for an inflammatory response in human aortic endothelial cells. (bu.edu)
- Different suppurative T. pyogenes infections in livestock and companion animals are associated with a variety of virulence factors, particularly exotoxin pyolysin and adhesins (fimbriae, neuraminidases, and collagen-binding protein) [ 6 ]. (hindawi.com)
- Specificity was confirmed by demonstrating that adhesion to the total lipids could be inhibited by pretreatment of the immobilized lipids with K88 fimbriae. (asm.org)
- Inhibition of S-fimbria-mediated adhesion to human ileostomy glycoproteins by a protein isolated from bovine colostrum. (colostrumresearch.org)
- The aim of this study was to isolate and purify the component in bovine colostrum which is responsible for the inhibition of S-fimbria-mediated adhesion of Escherichia coli. (colostrumresearch.org)
- 10K fractions and the colostral whey were tested for inhibition of in vitro adhesion of radiolabelled S-fimbria-bearing E. coli to human ileostomy glycoproteins, which provide a model for human intestinal mucus. (colostrumresearch.org)
- The pathogenic mechanism of the bacteria are not fully elucidated especially its potential activity of the protein as hemagglutinin and adhesion molecule. (unej.ac.id)
- After identification, bacterial isolate of fimbria fraction 12,5 % SDS-PAGE were used to isolate fimbria protein followed by hemagglutinin test and in vitro adhesion test. (unej.ac.id)
- The 45 kDa fimbriae protein was also adhesion protein showed by its activity to adherence to rabbit vesica urinaria epithel receptor. (unej.ac.id)
- The term "fimbria" can refer to many different (structural) types of pilus, as many different types of pili have been used for adhesion, a case of convergent evolution. (wikipedia.org)
- Genomic comparison between strains showed differences in regions containing a fimbriae/adhesion virulence protein. (ajtmh.org)
- 1991. Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces. (springer.com)
- PACs in cranberries are said to inhibit the adhesion capabilities of the fimbriae, causing the inhibited bacteria to pass through the urethra. (todaysdietitian.com)
- P-fimbriae is a strain, or adhesion protein, in E. coli , which has bacterial tips that bind to the uroepithelium. (todaysdietitian.com)
- These include bacterial cell aggregation, adhesion to surfaces of host cells such as, in the case of gut bacteria, the linings of the intestine, adhesion to other microbial cells in biofilms , gene and protein injection into other cells, DNA uptake by naturally transformable bacteria, and virulence attributes of pathogenic bacteria. (citizendium.org)
- Type 1 fimbriae are a crucial factor for the virulence of uropathogenic Escherichia coli during the first steps of infection by mediating adhesion to epithelial cells. (nih.gov)
- Some PilB‐like and PilQ‐like proteins are apparently also involved in the assembly of non‐type 4 polymeric structures (filamentous phage virions and conjugative pili). (ingentaconnect.com)
- An important group of biofilm matrix-associated proteins are those that polymerize into fibers variously known as pili or fimbriae ( 9 ). (pnas.org)
- For example, bacteria adhere to cells with hairlike fimbriae (pili). (todaysdietitian.com)
- Escherichia coli cell with bacterial fimbriae (pili). (citizendium.org)
- pili ) is a hairlike protein structure on the surface of a cell , especially Gram-negative bacteria . (citizendium.org)
- The terms pili and fimbriae are often used interchangeably. (citizendium.org)
- However, all types of pili are primarily composed of oligomeric pilin proteins which form the pilus rod structure. (citizendium.org)
- F-pili are involved in bacterial conjugation and connect the bacterium to another bacterium and enable a bridge between the cytoplasms of the cells which becomes the channel for one way transfer of a single-strand of DNA and certain protein molecules. (citizendium.org)
- A wide variety of different types of pili have been discovered whose biological roles include being used as molecular machines to transfer proteins and DNA between different cells. (citizendium.org)
Outer membrane protein2
- This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)-cholera toxin B subunit (CTB) in the intranasal murine model of infection. (biomedsearch.com)
- These strains differed only in that the latter one cannot produce intact K88 fimbriae because of a deletion in the gene coding for the major fimbrial subunit. (asm.org)
- Here, the recombinant production of a soluble, self-complemented construct of the LpfD protein of E. coli LF82 is reported and it is demonstrated that it forms the adhesive tip subunit of LPF. (ugent.be)
- The transformed strain expressing a plasmid containing genes encoding the WT fimbrial subunit and the prepilin peptidase displayed all of the hallmarks of a fimbriated bacterium including the distinct star-like colony morphology, robust biofilm formation, biofilm architecture composed of discrete microcolonies and the presence of fimbriae. (microbiologyresearch.org)
- Further characterization of this phenotype, by using antiserum raised against the LpfA1 putative major fimbrial subunit and by addition of mannose, showed that a reduced binding of EHEC to ECM proteins was observed in a long polar fimbria (lpf) mutant. (utmb.edu)
- Purified Lpf major subunit bound to all of the ECM proteins tested. (utmb.edu)
- Actinomyces naeslundii genospecies 1 and 2 express type-2 fimbriae (FimA subunit polymers) with variant Galβ binding specificities and Actinomyces odontolyticus a sialic acid specificity to colonize different oral surfaces. (biomedcentral.com)
- In addition, a sortase independent oligomerization of FimA subunit proteins in solution was indicated. (biomedcentral.com)
- The major subunit genes of type-2 and type-1 fimbriae, fimA and fimP , respectively, have been cloned and sequenced from A. naeslundii genospecies 1 (strain 12104) and 2 (strain T14V) [ 22 - 26 ]. (biomedcentral.com)
- The deduced FimA and FimP subunit proteins are 534 and 533 amino acid proteins, respectively, with 34 % amino acid identity. (biomedcentral.com)
- This was carried out by introduction of new restriction sites by PCR-mediated site-directed mutagenesis of fimA in positions predicted to correspond to optimally surface-located regions of the subunit protein. (nih.gov)
- An epitope from the B subunit of LT toxin (LTP1, 8 LCSEYRNTQIYTIN 21 ) and an STa toxoid epitope ( 5 CCELCCNPQCAGCY 18 ) were embedded in the FaeG major subunit of E. coli K88ac fimbriae. (asm.org)
- The results suggest that a third of rough-colony strains harbor ChoP and that ChoP is attached to fimbrial subunit protein Flp1. (diva-portal.org)
- FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. (uniprot.org)
- Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli K88 fimbriae. (asm.org)
- Therefore we conjugated fimbrial protein with a core oligosaccharide fraction obtained from Escherichia coli K-12 lipopolysaccharide, which has been found to contain an epitope common for several enterobacterial species [ 2 ]. (biomedcentral.com)
- Display of chimeric fimbriae was tested with respect to host background in three different Escherichia coli strains, i.e. an isogenic set of K-12 strains, differing in the presence of an indigenous fim gene cluster, as well as a wild-type isolate. (nih.gov)
- The 987P-like CS18 fimbriae of human enterotoxigenic Escherichia coli ON phase variants. (upenn.edu)
- Fimbriae are one of the primary mechanisms of virulence for E. coli, Bordetella pertussis, Staphylococcus and Streptococcus bacteria. (wikipedia.org)
- Woodward, MJ (2002), "The role of type 1 and curli fimbriae of Shiga toxin-producing Escherichia coli in adherence to abiotic surfaces", Int J Med Microbiol, 292 (3-4): 195-205, doi:10.1078/1438-4221-00203, PMID 12398210. (wikipedia.org)
- It is a fusion protein N - Lpp aa's 1-9 - OmpA aa's 46-159 - surface protein - C. E. coli K12 genome and BioBrick primers for the full OmpA gene (start through stop) have been ordered. (openwetware.org)
- Data from this study demonstrated that K88ac fimbriae expressing LT and STa epitope antigens elicited neutralizing anti-toxin antibodies and anti-adhesin antibodies and suggested that E. coli fimbriae could serve as a platform for the development of broad-spectrum vaccines against ETEC. (asm.org)
- To determine whether the antiserum against A.actinomycetemcomitans reacted with a protein of the sonic extract of the E.coli clone w … More as analyzed by Western blot analysis. (nii.ac.jp)
- Structure and function of periplasmic chaperone-like proteins involved in the biosynthesis of K88 and K99 fimbriae in enterotoxigenic Escherichia coli. (nii.ac.jp)
- The ability of E. coli strands to colonize the urinary tract is dependent on the presence of the P fimbriae, confirming its role in adhering to the urinary tract wall (15). (ecochildsplay.com)
- Some of the more frequently encountered fimbriae, such as coli surface antigen 1 (CS1), have been characterized in considerable detail. (biomedcentral.com)
- Type 1 fimbriae, a colonization factor of uropathogenic Escherichia coli, are controlled by the metabolic sensor CRP-cAMP. (nih.gov)
- Fimbriae mediate the attachment of many pathogenic bacteria to host cells. (biomedcentral.com)
- Elaboration of surface antigens by adherent bacteria was assayed by fimbriae- and flagella-specific ELISAs. (nih.gov)
- The highly conserved pilin, E box and LPXTG motifs are present in fimbriae proteins from other Gram-positive bacteria. (biomedcentral.com)
- e00955-18 ) used a cobalamin activity-based probe (B 12 -ABP) to demonstrate that B 12 -auxotrophic bacteria and archaea can be cultured on the B 12 -ABP, and they present results showing how the probe transports into cells and interacts with protein, RNA, and DNA. (asm.org)
- the chimeric proteins were exposed on the bacterial surface and the cholera toxin epitope was authentically displayed, i.e. it was recognized on bacteria by specific antiserum. (nih.gov)
- Negatively stained preparations incubated with PRP-gold conjugates but not bovine serum albumin-gold complexes bound specifically to bacteria possessing type 1 fimbriae, A. viscosus T14V-J1 and 5519. (wikigenes.org)
- MorC is a 141 kDa inner membrane protein that is conserved in Gram-negative bacteria. (uvm.edu)
- Therefore, bacterial fimbriae play an important role in first stage of the pathogenesis caused by pathogenic bacteria. (nii.ac.jp)
- In bacteriology, a fimbria (Latin for 'fringe', plural fimbriae), also referred to as an "attachment pilus" by some scientists, is a short appendage found on many Gram-negative and some Gram-positive bacteria, and that is thinner and shorter than a flagellum. (wikipedia.org)
- Fimbriae are used by bacteria to adhere to one another and to adhere to animal cells and some inanimate objects. (wikipedia.org)
- This layer, called a pellicle, consists of many aerobic bacteria that adhere to the surface by their fimbriae. (wikipedia.org)
- Thus, fimbriae allow the aerobic bacteria to remain both on the broth, from which they take nutrients, and near the air. (wikipedia.org)
- The fimbrial usher protein is involved in biogenesis of the pilus in Gram-negative bacteria. (wikipedia.org)
- Fimbriae are protein appendages used by bacteria to attach to other cells. (cichlidresearch.com)
- The fimbriae are hair-like structures protruding from the bacterial cell and by attaching to specific receptors in the urinary tract they mediate adherence to different cell types, allowing the bacteria to resist the shear forces from urine flow. (diva-portal.org)
- Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
- Several different attachment mechanisms have been identified for oral bacteria (i.e., through surface proteins, such as glucosyltransferases [GTF] and glucan-binding proteins, by sucrose-dependent mechanisms and through surface antigen P1 and/or fimbriae in sucrose-independent functions). (asm.org)
- In some cases, mutant pathogenic bacteria that lack fimbriae cannot adhere to their usual target host cell surfaces, and thus cannot cause disease . (citizendium.org)
- These lectins are one of the many types of adhesin protein produced by bacteria. (citizendium.org)
- Moreover, the underlying studies revealed that CRP-cAMP controls the expression of another global regulator in Gram-negative bacteria, the leucine-responsive protein Lrp. (nih.gov)
- AIEC strains express long polar fimbriae (LPF), which are known to target Peyer's patches in a mouse CD model. (ugent.be)
- To gain an understanding of the role of fimbriae and flagella in the adherence of Salmonella enterica serotype Enteritidis to inanimate surfaces, the extent of adherence of viable wild-type strains to a polystyrene microtitration plate was determined by a crystal violet staining assay. (nih.gov)
- Mutant strains unable to elaborate SEF14 and SEF17 fimbriae adhered poorly at 37 degrees C and 25 degrees C, respectively, but adherence was not abolished. (nih.gov)
- Non-motile mutant strains showed reduced adherence whilst type-1, PEF and LPF fimbriae appeared not to contribute to adherence in this assay. (nih.gov)
- However, the fimbrial nature of the sialic acid binding property and sequence information about FimA proteins from multiple strains are lacking. (biomedcentral.com)
- Three unique subtypes of FimA proteins with 63.8-66.4% sequence identity were present in strains of A. naeslundii genospecies 1 and 2 and A. odontolyticus . (biomedcentral.com)
- Moreover, only strains of genospecies 1 were agglutinated with type-2 fimbriae antisera derived from A. naeslundii genospecies 1 strain 12104, emphasizing that the overall folding of FimA may generate different functionalities. (biomedcentral.com)
- Binding of the PRP-gold probes to strains deficient in type 1 fimbriae, i.e., strains 5951 (type 2 fimbriae only) and 147 (no fimbriae), was negligible. (wikigenes.org)
- Interestingly, this study also indicated that the amounts of the individual protein components of the leukotoxin secretion apparatus are unchanged when comparing the wild type and mutant strains. (uvm.edu)
- 1984. The function and distribution of different fimbriae on the strains of Actinomyces viscousus and Actinomyces naeslundii . (springer.com)
- Bacterial invasion was observed only for strains carrying the P.IA subtype of porin, which is typically associated with the development of disseminated neisserial disease, and did not require opacity outer membrane proteins, previously recognized as gonococcal invasins. (rupress.org)
- Dominant low responsiveness in the IgG response of mice to the complex protein antigen type 1 fimbriae from Actinomyces viscosus T14V. (jimmunol.org)
- Our study concentrates on a glycoconjugate vaccine based on covalently attached bacterial antigen to Klebsiella fimbriae type 1 and 3. (biomedcentral.com)
- 14. Immunogenic Complex according to claim 11, whereby expression of the DNA transcription unit, that encodes an Antigen, in a host cell leads to production of a protein which is capable of inducing an immune response against said Antigen. (freepatentsonline.com)
- Bennick A. Salivary proline-rich proteins. (springer.com)
- Type 1 fimbriae have been purified from a Salmonella typhi strain of clinical origin. (nih.gov)
- In Salmonella , the phenomenon of rugosity (also called rdar or red, dry, and rough phenotype) has been investigated and shown to require curli ( 28 , 37 ), proteinaceous fibrils ( 7 ), also referred to as thin aggregative fimbriae or Tafi ( 35 ), and cellulose, which largely comprise the organism's exopolymeric substances (EPS) ( 37 ). (asm.org)
- It can be concluded that 35-day-old piglet mucus contains both protein and glycolipid receptors specific for K88 fimbriae, the latter being galactosylceramide. (asm.org)
- Extracellular matrix (ECM) proteins might act as receptors involved in the recognition of enteric pathogens, including EHEC. (utmb.edu)
- In many cases, the adherence takes place through the binding of fimbriae to specific receptors on the host cell surface. (nii.ac.jp)
- We have previously demonstrated that the fimbriae attaches to monocytes and fibroblasts through the binding of the fimbriae to specific receptors, and induce inflammatory cytokines that stimulate bone resorption. (nii.ac.jp)
- 1988. Adsorbed salivary proline-rich protein-1 and statherin: receptors for type 1 fimbriae of Actinomyces viscousus T14V-J1 on apatitic surfaces. (springer.com)
- This suggests that MorC is involved in fimbriae secretion and we have demonstrated the importance of this protein in fimbriae biogenesis and biofilm formation. (uvm.edu)
- Given the importance of TasA in biofilm formation, we characterized the physicochemical properties of this protein. (pnas.org)
- When added to a culture of a tasA mutant, purified TasA restored wild-type biofilm morphology, indicating that the purified protein retained biological activity. (pnas.org)
- Its simultaneous expression of multiple fimbriae enables colonization and biofilm formation. (diva-portal.org)
- CSE up-regulates P. gingivalis FimA at the protein level, suppresses the production of capsular polysaccharides at the ultrastructural level, and creates conditions that promote biofilm formation. (nih.gov)
- We also provide evidence that the repression of type 1 fimbriae by CRP-cAMP occurs during fast growth conditions (logarithmic phase) and is alleviated during slow growth (stationary phase), which is consistent with an involvement of type 1 fimbriae in the adaptation to stress conditions by promoting biofilm growth or entry into host cells. (nih.gov)
- Custom ELISA Kits, Recombinant Proteins and Antibodies can be designed, manufactured and produced according to the researcher's specifications. (mybiosource.com)
- Similarly, chimeric fimbriae with an STa peptide expressed in ETEC adhesin CS31A elicited neutralizing anti-STa antibodies ( 4 , 5 ). (asm.org)
- We have isolated a mixture of S. mutans surface proteins, which contained fimbria components (fimbria-enriched preparation), as demonstrated by immunostaining and electron microscopy, and have elicited antibodies in rabbits against this preparation ( 7 ). (asm.org)
- An in vitro microbial model ( 5 ) was used to demonstrate, for the first time, the efficacy of antibodies against the fimbria-enriched preparation in preventing the formation of carious lesions ( 6 ). (asm.org)
Expression of type 1 fimbriae2
- The effect of FocB on expression of type 1 fimbriae was also tested. (diva-portal.org)
- Our work suggests that the metabolic sensor CRP-cAMP plays a role in coupling the expression of type 1 fimbriae to environmental conditions, thereby also affecting subsequent attachment and colonization of host tissues. (nih.gov)
- Receptor activity specific for the K88 fimbriae was demonstrated in the lipids extracted from mucus, as the neutral lipids contained six times as much receptor activity as the acidic lipid fraction. (asm.org)
- LpfD binding was found to be resistant to treatment with O- or N-glycosidases, but was lost in collagenase-treated tissue sections, indicating the possible involvement of an intestinal matrix-associated protein as the LpfD receptor. (ugent.be)
- The protein appears to block receptor sites for S- fimbriae on ileostomy glycoproteins, with steric hindrance being the most likely mechanism. (colostrumresearch.org)
- The gene clusters of R-tailocins (bacteriocins resembling contractile bacteriophage tails) typically encode one receptor-binding tail fiber protein per R-tailocin, which determines target specificity. (asm.org)
- Agglutinin and acidic proline-rich protein receptor patterns may modulate bacterial adherence and colonization on tooth surfaces. (springer.com)
- The first step in the initiation of infection by this pathogenic bacterium is its attachment (i.e., through bacterial surface proteins such as glucosyltransferases, P1, glucan-binding proteins, and fimbriae) to a suitable receptor. (asm.org)
- These foci can be detected underneath bound CO particles within 30 seconds of cell activation, and their formation requires active protein kinase C. Complement receptor-mediated internalization requires intact microtubules and is accompanied by the accumulation of vesicles beneath the forming phagosome . (imm.org)
- By contrast, during the ingestion of AO particles (Fcgamma receptor mediated phagocytosis), all proteins are uniformly distributed on or near the phagosome surface. (imm.org)
- The adhesive properties of YapV and paralogous autotransporter proteins of Yersinia pestis. (upenn.edu)
- In the original and later autotransporter papers, the passenger protein was mutated to lack cysteines in order to prevent disulfide bond formation in the periplasm: tertiary structure prevents passage of the protein through the beta barrel. (openwetware.org)
- These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene, FimA. (uniprot.org)
- p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors. (uniprot.org)
- This morphology is associated with the expression of a novel gene, morC (morphogenesis protein C), identified in my laboratory. (uvm.edu)
- and a method for gene therapy comprising administering to an animal a GM1-binding protein and a functional polynucleotide and expressing the polynucleotide in the animal whereby the function of the polynucleotide confers on the animal a therapeutic effect. (google.it)
- Publications] Murakami,Y.: 'N-acetyl-D-galactosamine inhibits TNF-a gene expression induced in mouse peritonalmacrophages by fimbriae of' Biochem.Biophys.Res.Commun.192. (nii.ac.jp)
- The Gene Ontology system does not treat fimbriae as a distinct type of appendage, using the generic pilus (GO:0009289) type instead. (wikipedia.org)
- 7 , 8 The presence of some of these genes has been related to virulence factors such as the fliC gene (flagellin), papC gene (fimbria P) and kpsMTII gene (capsule). (dovepress.com)
- In the present study, astrocytes from transgenic mice that express enhanced green fluorescent protein (eGFP) under the control of a bacterial artificial chromosome (BAC) containing a very large region of DNA surrounding the GLT-1 gene (BAC GLT-1 eGFP mice) were used to assess the role of nuclear factor-κB (NF-κB) in neuron-dependent activation of the GLT-1 promoter. (jneurosci.org)
- B. bronchiseptica" expresses many protein factors, such as filamentous haemagglutinin (FHA), pertactin and fimbriae. (kenyon.edu)
- Taken together, these findings suggest that the interaction of Lpf and ECM proteins contributes to the EHEC colonization of the gastrointestinal tract. (utmb.edu)
- The focus of my research is the identification and characterization of bacterial proteins that are required for tissue colonization, the initiation of any infection. (uvm.edu)
- We also found that the two regulators, H-NS and Ler, had an effect in EHEC Lpf-mediated binding to ECM, supporting the roles of these tightly regulated fimbriae as adherence factors. (utmb.edu)
- Finally, increased bacterial adherence was observed when T84 cells, preincubated with ECM proteins, were infected with EHEC. (utmb.edu)
- Bacterial fimbriae may be an important cell structure for the adherence. (nii.ac.jp)
- It is our belief that fimbriae are important virulence factors for S. mutans and are at least partially responsible for S. mutans sucrose-independent adherence to enamel surfaces. (asm.org)
- Pathogenicity is also dependent upon the expression of fimbriae, which function as adherence factors, allowing the pathogen to colonize the small intestine. (biomedcentral.com)
- The recently reported finding that K88 fimbriae bind to glycoproteins in mucus from the piglet small intestine was confirmed in two ways. (asm.org)
- Purified fimbriae retained their ability to bind to erythrocytes in a mannose-inhibitable fashion and, in doing so, behaved preferentially as a monovalent adhesin. (nih.gov)
- One thing to consider: in the absence of aptamers that bind to the cell surface, we might as well try it with aptamers that bind proteins in vitro as done in 12000850. (openwetware.org)
- FimA binds to mannose sugars present on a variety of different host cell surface structures and can be considered to be a lectin , the general name for proteins that bind to sugar residues  . (citizendium.org)
- The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). (biomedsearch.com)
- The mechanisms by which S. suis constructs these fimbria-like structures on the cell surface and adheres to host cells were also elucidated. (asm.org)
- It seems likely that the Pil homologues function in the processing and export of proteins resembling type 4 fimbrial sub‐units, and in their organization into fimbrial‐like structures. (ingentaconnect.com)
- My research interests are concentrated on the membrane proteins and surface structures of the Gram-negative bacterium Aggregatibacter actinomycetemcomitans . (uvm.edu)
- The extracellular matrix protein adhesin A (EmaA) is a 202 kDa protein that trimerizes to form antenna-like structures on the surface of the bacterium. (uvm.edu)
- These structures are mainly composed of tubulin proteins supported by microfilaments and microtubules. (cichlidresearch.com)
- tubulare, hairlike structures of protein larger and more rare than fimbriae. (powershow.com)
- These are structures (not present in all prokaryotes) made of proteins that facilitate movement and communication between cells. (wikipedia.org)
- Fimbriae have been identified on numerous gram-negative microorganisms as long fibrillar structures but have been reported for only a limited number of gram-positive microorganisms, including some oral streptococci, in which they typically appear as a much shorter fuzzy coat ( 4 , 21 ). (asm.org)
- During the ingestion of CO particles, punctate structures rich in F-actin, vinculin, alpha-actinin, paxillin, and phosphotyrosine-containing proteins are distributed over the phagosome surface . (imm.org)
- The eukaryotic flagellum is host to a variety of dynamic behaviors, including flagellar beating, the motility of glycoproteins in the flagellar membrane, and intraflagellar transport (IFT), the bidirectional traffic of protein particles between the flagellar base and tip. (cichlidresearch.com)
- Once a tooth surface is cleaned, a conditioning film of proteins and glycoproteins is adsorbed rapidly to the tooth surface. (springer.com)
- Binding of Porphyromonas gingivalis fimbriae to proline-rich glycoproteins in parotid saliva via a domain shared by major salivary components. (springer.com)
- P fimbriae Pilus Proft, T. (wikipedia.org)
- fimbriae ) is a short pilus. (citizendium.org)
- They are composed of several different protein components including FimH adhesin that is part of the short thin fibrillar pilus tip, and FimA which makes up the thicker 7 nm thick long rod of the pilus. (citizendium.org)
- The assembly of the Type I pilus occurs in the periplasm and involves the "chaperone/usher" protein folding and delivery system. (citizendium.org)
- A recent quantitative proteomics study employing an afimbriated strain and a developed mutant isogenic for the inner-membrane protein morphogenesis protein C (MorC) revealed that the abundance of the proteins of the fimbrial secretion apparatus in the membrane is dependent on MorC. (microbiologyresearch.org)
- There was, however, a reduction in the secretion of fimbrial subunits, and fewer fimbriae were observed on the surface of the mutant strain. (microbiologyresearch.org)
- All three FimA protein variants contained a signal peptide, pilin motif, E box, proline-rich segment and an LPXTG sorting motif among other conserved segments for secretion, assembly and sorting of fimbrial proteins. (biomedcentral.com)
- Pil‐like proteins are also involved in protein secretion, DNA transfer by conjugation and transformation, and morphogenesis of filamentous bacteriophages. (ingentaconnect.com)
- In addition, the absence of this protein abolishes the secretion of leukotoxin, a toxin that specifically targets human white blood cells. (uvm.edu)
- This suggests that the absence of MorC results in a conformation change of one or more of the proteins associated with the secretion apparatus, which leads to reduced secretion of the toxin. (uvm.edu)
- The same data set has also revealed a decrease in multiple fimbriae secretion apparatus proteins. (uvm.edu)
- The Pseudomonas aeruginosa genes pilB‐D and pilQ are necessary for the assembly of type 4 fimbriae. (ingentaconnect.com)
- The emphasis of my research is based on two genes and the associated proteins identified in my laboratory. (uvm.edu)
- Previous studies have indicated that there is a co-regulation between different fimbrial genes and one factor that has been implicated in this is the PapB protein, acting as a transcriptional regulator of P-fimbrial expression. (diva-portal.org)
- In this study, the role of the FocB protein in the regulation of F1C fimbriae as well as in the co-regulation with other fimbrial genes was investigated. (diva-portal.org)
- The genus Actinomyces involves a diversity of unique FimA proteins with conserved pilin, E box and LPXTG motifs, depending on subspecies and associated binding specificity. (biomedcentral.com)
- To investigate further the relationship between MorC and fimbriation, we identified and complemented the defect in fimbriae production in the afimbriated laboratory strain. (microbiologyresearch.org)
- We have completed a quantitative proteomic survey comparing the membrane proteins of the wild type with the morC mutant strain. (uvm.edu)
- The study has revealed that the phenotypes displayed by the morC mutant strain are due to changes in the abundance of a small cohort of membrane proteins. (uvm.edu)
- The TEPC-15-reactive protein was undetectable in flp1 mutant strain. (diva-portal.org)
- 3 Formation of MAC is under control of two inhibitors, the membrane protein CD59 and the multifunctional glycoprotein vitronectin that is found both in plasma and in the extracellular matrix (ECM) ( 1 ). (jimmunol.org)
- The link of high cytosolic glucose concentration to metabolic dysfunction was demonstrated by overexpression of the GLUT1 glucose transporter in renal mesangial cells that thereby acquired the characteristics of the diabetic phenotype, including increased extracellular matrix protein synthesis and activation of the polyol pathway ( 7 ). (diabetesjournals.org)
- Although the specific composition of the extracellular matrix also varies from species to species, polysaccharides, proteins, and nucleic acids are important components of the matrix that help maintain the structural integrity of biofilms, mediating both cell-to-cell and cell-to-surface interactions ( 3 - 8 ). (pnas.org)
- This organization is largely dependent upon the presence of the extracellular matrix, which is composed mainly of an exopolysaccharide and the protein TasA ( 25 ). (pnas.org)
- It was later shown that TasA is indeed the major protein component of the extracellular matrix of B. subtilis biofilms ( 25 ). (pnas.org)
- 1986a and b) also provided evidence for extracellular fungal stuctures, called "fungal fimbriae", which the authors described as primarily proteinaceous. (springer.com)
- Serum intestinal fatty acid-binding protein in the noninvasive diagnosis of celiac disease. (bireme.br)
- The purpose of this study is to measure the levels of serum proteins and other biomarkers in cancer patients and in patients suspected of having cancer. (stanford.edu)
- 0.05) higher salivary IgA and serum IgG antibody responses to the mixture of surface proteins and to whole bacterial cells than did the other two groups (B and C). No significant differences were found in the average numbers of recovered S. mutans cells among groups. (asm.org)
- 1983. Effect of packing density and polysaccharide to protein ratio of plaque samples cultured in vitro upon their permeability. (springer.com)
- Conformational changes in salivary proline- rich protein 1 upon adsorption to calcium phosphate crystals. (springer.com)
- FimA and FimP contain seven conserved proline-containing regions involved in folding of the two proteins and an LPXTG sorting signal followed by a N-terminal membrane spanning domain [ 25 ]. (biomedcentral.com)
- The mechanism by which this critical protein functions is unclear and the long term goal of this arm of my research is to define how MorC and associated proteins determine membrane structure and function in this oral pathogen. (uvm.edu)
- Cell surface polysacebaride and outer membrane proteins upregulated the IL-6 production by human gingival fibroblast cell lines as well as LPS.These activities were dependent on the increase of the preparations dose. (nii.ac.jp)
- Cells consist of cytoplasm enclosed within a membrane , which contains many biomolecules such as proteins and nucleic acids . (wikipedia.org)
- They correspond to functions allowing opportunistic pathogens to colonise the mucus layers, breach the host barriers and invade the mucosae, which could still be aggravated by decreased host-derived pancreatic zymogen granule membrane protein GP2 in CD patients. (bmj.com)
- HfsK is a soluble protein but associates with the cell membrane during most of the cell cycle. (asm.org)
- The curli are synthesized at stationary phase below 30°C ( 28 ), while cellulose is assembled only at temperatures below 30°C because it requires the temperature-sensitive regulatory protein AdrA for complete biosynthesis ( 37 ). (asm.org)
- Design We first developed and validated a workflow-including extraction of microbial communities, two-dimensional difference gel electrophoresis (2D-DIGE), and LC-MS/MS-to discover protein signals from CD-associated gut microbial communities. (bmj.com)
- The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. (diva-portal.org)
- In order to isolate pure fimbriae, simple methods have been adopted, especially considering the contamination with lipopolysaccharide. (biomedcentral.com)
- Surface proteinaceous fibrils (fimbriae) on filamentous fungi. (springer.com)
- Bacterial fimbriae have been defined as small (100 to 300 nm), nonflagellar, filamentous, proteinaceous surface appendages that do not participate in the transfer of bacterial or viral nucleic acids ( 1 ). (asm.org)
- A Western blot analysis of the purified fimbrial preparation using an antiserum raised against native fimbriae suggested that fimbrial proteins did not carry any major sequential epitope and that, in native fimbriae, conformational epitopes, possibly generated between different subunits, might provide for the major immunogenic epitopes. (nih.gov)
- If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
- 4. The composition of Claim 1 wherein the mucosa-binding protein is an antibody. (google.it)
- 2) The fimbria-stimulated bone resorption was inhibited markedly by antibody against the fimbrias. (nii.ac.jp)
- Activation of this system leads to a deposition of complement proteins on the bacterial surface, which results in opsonization of pathogens. (jimmunol.org)
- p>This indicates the type of evidence that supports the existence of the protein. (uniprot.org)
- The mannose-specific fimbriae of type 1 are expressed in many enterobacterial species. (biomedcentral.com)
- The type 3 of Klebsiella fimbriae are moderate inductors of IL-6 and interferon, whereas type 1 is even less potent inductor. (biomedcentral.com)
- These results prompt us for studies on practical use of fimbriae, especially type 1, as carriers for conjugate vaccine. (biomedcentral.com)
- Common components in the assembly of type 4 fimbriae, DNA transfe. (ingentaconnect.com)
- These may either be true type 4 fimbriae, or components of protein complexes which act in the transport of macromolecules (DNA or protein) into or out of the cell. (ingentaconnect.com)
- Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae: effects of insert position and host background. (nih.gov)
- The potential of the major structural protein of type 1 fimbriae as a display system for heterologous sequences was tested. (nih.gov)
- Curli are a type of fimbriae. (wikipedia.org)
- Another type are called type 1 fimbriae. (wikipedia.org)
- The chaperone-usher pathway is responsible for moving many types of fimbriae out of the cell, including type 1 fimbriae and the P fimbriae. (wikipedia.org)
- 3. The composition of Claim 1 wherein the mucosa-binding protein is selected from the group consisting of cholera toxin and its derivatives. (google.it)