Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
The genetic complement of MITOCHONDRIA as represented in their DNA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).
Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).
Proteins obtained from ESCHERICHIA COLI.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Infections with bacteria of the species ESCHERICHIA COLI.
A species of gram-negative, anaerobic, rod-shaped bacteria originally classified within the BACTEROIDES genus. This bacterium produces a cell-bound, oxygen-sensitive collagenase and is isolated from the human mouth.
Protein domains that are enriched in PROLINE. The cyclical nature of proline causes the peptide bonds it forms to have a limited degree of conformational mobility. Therefore the presence of multiple prolines in close proximity to each other can convey a distinct conformational arrangement to a peptide chain.
Infections with bacteria of the family BACTEROIDACEAE.
A genus of gram-negative, anaerobic, nonsporeforming, nonmotile rods or coccobacilli. Organisms in this genus had originally been classified as members of the BACTEROIDES genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Bacteroides species, and hence, this new genus was created.
Oral tissue surrounding and attached to TEETH.
A plant genus of the family OLEACEAE. Members contain secoiridoid glucosides.
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
Instructional materials used in teaching.
The branch of pharmacology that deals directly with the effectiveness and safety of drugs in humans.
The direct struggle between individuals for environmental necessities or for a common goal.
A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.
The most well known avian paramyxovirus in the genus AVULAVIRUS and the cause of a highly infectious pneumoencephalitis in fowl. It is also reported to cause CONJUNCTIVITIS in humans. Transmission is by droplet inhalation or ingestion of contaminated water or food.
Biological actions and events that constitute the steps by which living organisms take in and assimilate NUTRIENTS.
Uptake of substances through the lining of the INTESTINES.
An acute febrile, contagious, viral disease of birds caused by an AVULAVIRUS called NEWCASTLE DISEASE VIRUS. It is characterized by respiratory and nervous symptoms in fowl and is transmissible to man causing a severe, but transient conjunctivitis.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
The interactions between a host and a pathogen, usually resulting in disease.
The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
Acute illnesses, usually affecting the GASTROINTESTINAL TRACT, brought on by consuming contaminated food or beverages. Most of these diseases are infectious, caused by a variety of bacteria, viruses, or parasites that can be foodborne. Sometimes the diseases are caused by harmful toxins from the microbes or other chemicals present in the food. Especially in the latter case, the condition is often called food poisoning.
A species of bacteria that resemble small tightly coiled spirals. Its organisms are known to cause abortion in sheep and fever and enteritis in man and may be associated with enteric diseases of calves, lambs, and other animals.
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
A genus of gram-negative opportunistic foodborne pathogens.
A species of gram-negative bacteria in the genus CHRONOBACTER, found in the environment and in foods.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Adherence of cells to surfaces or to other cells.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A subgenus of Salmonella containing several medically important serotypes. The habitat for the majority of strains is warm-blooded animals.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Infections in animals with bacteria of the genus SALMONELLA.

Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract. (1/2491)

During colonization of the respiratory tract by Bordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal epithelial cell line HEp-2. Using B. pertussis mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of B. pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.  (+info)

Genetic characterization of a new type IV-A pilus gene cluster found in both classical and El Tor biotypes of Vibrio cholerae. (2/2491)

The Vibrio cholerae genome contains a 5.4-kb pil gene cluster that resembles the Aeromonas hydrophila tap gene cluster and other type IV-A pilus assembly operons. The region consists of five complete open reading frames designated pilABCD and yacE, based on the nomenclature of related genes from Pseudomonas aeruginosa and Escherichia coli K-12. This cluster is present in both classical and El Tor biotypes, and the pilA and pilD genes are 100% conserved. The pilA gene encodes a putative type IV pilus subunit. However, deletion of pilA had no effect on either colonization of infant mice or adherence to HEp-2 cells, demonstrating that pilA does not encode the primary subunit of a pilus essential for these processes. The pilD gene product is similar to other type IV prepilin peptidases, proteins that process type IV signal sequences. Mutational analysis of the pilD gene showed that pilD is essential for secretion of cholera toxin and hemagglutinin-protease, mannose-sensitive hemagglutination (MSHA), production of toxin-coregulated pili, and colonization of infant mice. Defects in these functions are likely due to the lack of processing of N termini of four Eps secretion proteins, four proteins of the MSHA cluster, and TcpB, all of which contain type IV-A leader sequences. Some pilD mutants also showed reduced adherence to HEp-2 cells, but this defect could not be complemented in trans, indicating that the defect may not be directly due to a loss of pilD. Taken together, these data demonstrate the effectiveness of the V. cholerae genome project for rapid identification and characterization of potential virulence factors.  (+info)

Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae. (3/2491)

Salmonella typhimurium exhibits a distinct tropism for mouse enterocytes that is linked to their expression of type 1 fimbriae. The distinct binding traits of Salmonella type 1 fimbriae is also reflected in their binding to selected mannosylated proteins and in their ability to promote secondary bacterial aggregation on enterocyte surfaces. The determinant of binding in Salmonella type 1 fimbriae is a 35-kDa structurally distinct fimbrial subunit, FimHS, because inactivation of fimHS abolished binding activity in the resulting mutant without any apparent effect on fimbrial expression. Surprisingly, when expressed in the absence of other fimbrial components and as a translational fusion protein with MalE, FimHS failed to demonstrate any specific binding tropism and bound equally to all cells and mannosylated proteins tested. To determine if the binding specificity of Salmonella type 1 fimbriae was determined by the fimbrial shaft that is intimately associated with FimHS, we replaced the amino-terminal half of FimHS with the corresponding sequence from Escherichia coli FimH (FimHE) that contains the receptor binding domain of FimHE. The resulting hybrid fimbriae bearing FimHES on a Salmonella fimbrial shaft exhibited binding traits that resembled that of Salmonella rather than E. coli fimbriae. Apparently, the quaternary constraints imposed by the fimbrial shaft on the adhesin determine the distinct binding traits of S. typhimurium type 1 fimbriae.  (+info)

P fimbriae and other adhesins enhance intestinal persistence of Escherichia coli in early infancy. (4/2491)

Resident and transient Escherichia coli strains were identified in the rectal flora of 22 Pakistani infants followed from birth to 6 months of age. All strains were tested for O-antigen expression, adhesin specificity (P fimbriae, other mannose-resistant adhesins or type 1 fimbriae) and adherence to the colonic cell line HT-29. Resident strains displayed higher mannose-resistant adherence to HT-29 cells, and expressed P fimbriae (P = 0.0036) as well as other mannose-resistant adhesins (P = 0.012) more often than transient strains. In strains acquired during the first month of life, P fimbriae were 12 times more frequent in resident than in transient strains (P = 0.0006). The O-antigen distribution did not differ between resident and transient strains, and none of the resident P-fimbriated strains belonged to previously recognized uropathogenic clones. The results suggest that adhesins mediating adherence to intestinal epithelial cells, especially P fimbriae, enhance the persistence of E. coli in the large intestine of infants.  (+info)

Environmental signals modulate ToxT-dependent virulence factor expression in Vibrio cholerae. (5/2491)

The regulatory protein ToxT directly activates the transcription of virulence factors in Vibrio cholerae, including cholera toxin (CT) and the toxin-coregulated pilus (TCP). Specific environmental signals stimulate virulence factor expression by inducing the transcription of toxT. We demonstrate that transcriptional activation by the ToxT protein is also modulated by environmental signals. ToxT expressed from an inducible promoter activated high-level expression of CT and TCP in V. cholerae at 30 degrees C, but expression of CT and TCP was significantly decreased or abolished by the addition of 0.4% bile to the medium and/or an increase of the temperature to 37 degrees C. Also, expression of six ToxT-dependent TnphoA fusions was modulated by temperature and bile. Measurement of ToxT-dependent transcription of genes encoding CT and TCP by ctxAp- and tcpAp-luciferase fusions confirmed that negative regulation by 37 degrees C or bile occurs at the transcriptional level in V. cholerae. Interestingly, ToxT-dependent transcription of these same promoters in Salmonella typhimurium was relatively insensitive to regulation by temperature or bile. These data are consistent with ToxT transcriptional activity being modulated by environmental signals in V. cholerae and demonstrate an additional level of complexity governing the expression of virulence factors in this pathogen. We propose that negative regulation of ToxT-dependent transcription by environmental signals prevents the incorrect temporal and spatial expression of virulence factors during cholera pathogenesis.  (+info)

Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of twitching motility. (6/2491)

Pseudomonas aeruginosa is a ubiquitous environmental bacterium and an important human pathogen. The production of several virulence factors by P. aeruginosa is controlled through two quorum-sensing systems, las and rhl. We have obtained evidence that both the las and rhl quorum-sensing systems are also required for type 4 pilus-dependent twitching motility and infection by the pilus-specific phage D3112cts. Mutants which lack the ability to synthesize PAI-1, PAI-2, or both autoinducers were significantly or greatly impaired in twitching motility and in susceptibility to D3112cts. Twitching motility and phage susceptibility in the autoinducer-deficient mutants were partially restored by exposure to exogenous PAI-1 and PAI-2. Both twitching motility and infection by pilus-specific phage are believed to be dependent on the extension and retraction of polar type 4 pili. Western blot analysis of whole-cell lysates and enzyme-linked immunosorbent assays of intact cells were used to measure the amounts of pilin on the cell surfaces of las and rhl mutants relative to that of the wild type. It appears that PAI-2 plays a crucial role in twitching motility and phage infection by affecting the export and assembly of surface type 4 pili. The ability of P. aeruginosa cells to adhere to human bronchial epithelial cells was also found to be dependent on the rhl quorum-sensing system. Microscopic analysis of twitching motility indicated that mutants which were unable to synthesize PAI-1 were defective in the maintenance of cellular monolayers and migrating packs of cells. Thus, PAI-1 appears to have an essential role in maintaining cell-cell spacing and associations required for effective twitching motility.  (+info)

The level of expression of the minor pilin subunit, CooD, determines the number of CS1 pili assembled on the cell surface of Escherichia coli. (7/2491)

CooD, the minor subunit of CS1 pili of enterotoxigenic Escherichia coli, is essential for the assembly of stable, functional pili. We previously proposed that CooD is a rate-limiting initiator of CS1 pilus assembly and predicted that the level of CooD expression should therefore determine the number of CS1 pili assembled on the cell surface. In this study, we confirm that CooD is required for the initiation of pilus assembly rather than for the stabilization of pili after they are assembled by demonstrating that specific modulation of cooD expression also modulates the number of CS1 pili on bacterial cells.  (+info)

Organization of biogenesis genes for aggregative adherence fimbria II defines a virulence gene cluster in enteroaggregative Escherichia coli. (8/2491)

Several virulence-related genes have been described for prototype enteroaggregative Escherichia coli (EAEC) strain 042, which has been shown to cause diarrhea in human volunteers. Among these factors are the enterotoxins Pet and EAST and the fimbrial antigen aggregative adherence fimbria II (AAF/II), all of which are encoded on the 65-MDa virulence plasmid pAA2. Using nucleotide sequence analysis and insertional mutagenesis, we have found that the genes required for the expression of each of these factors, as well as the transcriptional activator of fimbrial expression AggR, map to a distinct cluster on the pAA2 plasmid map. The cluster is 23 kb in length and includes two regions required for expression of the AAF/II fimbria. These fimbrial biogenesis genes feature a unique organization in which the chaperone, subunit, and transcriptional activator lie in one cluster, whereas the second, unlinked cluster comprises a silent chaperone gene, usher, and invasin reminiscent of Dr family fimbrial clusters. This plasmid-borne virulence locus may represent an important set of virulence determinants in EAEC strains.  (+info)

The Escherichia coli P fimbriae F71, F72, F9, and F11 from four cloned strains were purified, and polyclonal antisera were raised in rabbits. Cross-reactions of these antisera with eight different cloned and purified fimbriae were measured in an enzyme-linked immunosorbent assay. These antisera showed a reaction with the homologous fimbriae and also with most heterologous fimbriae. Monoclonal antibodies (MAbs) directed against the same four native fimbriae were produced by the fusion of spleen cells from immunized BALB/c mice with SP2/0 myeloma cells. The resulting four series of MAbs were also screened in an enzyme-linked immunosorbent assay with eight different cloned and purified fimbriae. Four different F71 hybridomas produced MAbs which recognized only epitopes on F71 fimbriae. Two F72 MAbs recognized epitopes on F72 and F9 fimbriae, whereas another F72 MAb recognized an epitope on only F72 fimbriae. Three MAbs raised against F9 reacted only with epitopes on F9 fimbriae. Six MAbs against ...
TY - JOUR. T1 - Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. AU - Yoshihara, Shizue. AU - Geng, Xiao Xing. AU - Okamoto, Shinobu. AU - Yura, Kei. AU - Murata, Takashi. AU - Go, Mitiko. AU - Ohmori, Masayuki. AU - Ikeuchi, Masahiko. PY - 2001/1/1. Y1 - 2001/1/1. N2 - The relevance of pilus-related genes to motility, pilus structure on the cell surface and competency of natural transformation was studied by gene disruption analysis in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. The genes disrupted in this study were chosen as related to the pil genes for biogenesis of the type IV pili in a Gram-negative bacterium Pseudomonas aeruginosa. It was found that motility of Synechocystis cells was lost in the mutants of slr0063, slr1274, slr1275, slr1276, slr1277 and sll1694 together with a simultaneous loss of the thick pili on the cell surface. Competency of the ...
TY - JOUR. T1 - Identification of bacterial factors involved in type 1 fimbria expression using an Escherichia coli K12 proteome chip. AU - Chen, Yi Wen. AU - Teng, Ching Hao. AU - Ho, Yu Hsuan. AU - Ho, Tien Yu Jessica. AU - Huang, Wen Chun. AU - Hashimoto, Masayuki. AU - Chiang, I. Yuan. AU - Chen, Chien Sheng. PY - 2014/6. Y1 - 2014/6. N2 - Type 1 fimbriae are filamentous structures on Escherichia coli. These structures are important adherence factors. Because binding to the host cells is the first step of infection, type 1 fimbria is an important virulence factor of pathogenic E. coli. Expression of type 1 fimbria is regulated by a phase variation in which each individual bacterium can alternate between fimbriated (phase-ON) and nonfimbriated (phase-OFF) states. The phase variation is regulated by the flipping of the 314-bp fimS fragment, which contains the promoter driving the expression of the genes required for the synthesis of type 1 fimbria. Thus, the bacterial proteins able to interact ...
Salmonella enterica serotype Typhimurium produces surface-associated fimbriae that facilitate adherence of the bacteria to a variety of cells and tissues. Type 1 fimbriae with binding specificity to mannose residues are the most commonly found fimbrial type. In vitro, static-broth culture favors the growth of S. Typhimurium with type 1 fimbriae, whereas non-type 1 fimbriate bacteria are obtained by culture on solid-agar media. Previous studies demonstrated that the phenotypic expression of type 1 fimbriae is the result of the interaction and cooperation of the regulatory genes fimZ, fimY, fimW, and fimU within the fim gene cluster. Genome sequencing revealed a novel gene, stm0551, located between fimY and fimW that encodes an 11.4-kDa putative phosphodiesterase specific for the bacterial second messenger cyclic-diguanylate monophosphate (c-di-GMP). The role of stm0551 in the regulation of type 1 fimbriae in S. Typhimurium remains unclear. A stm0551-deleted stain constructed by allelic exchange
The chaperone-usher (CU) pathway is a translocation system used to assemble adhesive multi-subunit fibres on the outer surface of gram-negative bacteria. CU pili are formed by the non-covalent polymerisation of several hundreds or thousands of pilus subunits which consist of an incomplete immunoglobulin (Ig)-like fold lacking the C-terminal ß-strand. In the periplasm, a cognate chaperone assists in pilus subunit folding by donating a b-strand to complement the truncated Ig-like fold of the pilus subunit, a process termed donor-strand complementation (Figure 69, A*) [1]. Chaperone:subunit complexes are then recruited to a pilus assembly platform in the outer membrane (OM) called the usher. The usher catalyses ordered subunit polymerisation and mediates translocation of the nascent pilus to the cell surface. Polymerisation of pilus subunits occurs through an intermolecular fold complementation mechanism involving the first 10-20 residues (termed N-terminal extension or Nte) of the pilus ...
Chaperone-usher (CU) fimbriae are adhesive surface organelles common to many Gram-negative bacteria. Escherichia coli genomes contain a large variety of characterised and putative CU fimbrial operons, however, the classification and annotation of individual loci remains problematic. Here we describe …
Bacterial pathogens utilize the chaperone-usher pathway to assemble extracellular multi-subunit fibers essential for virulence. The periplasmic chaperone facilitates the initial folding of fiber subunits but then traps them in activated folding transition states. Chaperone dissociation releases the folding energy that drives subunit incorporation into the fiber, which grows through a pore formed by the outer-membrane usher.. ...
The paper in Nature Microbiology can be found here. The work presented in this paper was the result of a strong collaboration between the laboratories of Dr. Scott J. Hultgren and Dr. Peng Yuan at Washington University in St. Louis. Chaperone-usher pathway (CUP) pili are extracellular proteinaceous fibers ubiquitously found on Gram-negative bacteria. Type 1 and P pili are produced by uropathogenic strains of E. coli and are tipped with the FimH and PapG adhesins, respectively, to mediate host and tissue tropism to the bladder and kidney. During pilus assembly hundreds of individual pilus subunits called pilins are first exported across the inner membrane to the periplasm. Chaperone-pilin complexes are then guided to an outer membrane (OM) nanomachine called the usher, which catalyzes subunit-subunit interactions. The usher contains five functional domains: a 24-stranded transmembrane β-barrel domain, a β-sandwich plug domain that resides in the pore of the TD in the apo-usher, an ...
Proteinaceous, nonflagellar surface appendages constitute a variety of structures, including those known variably as fimbriae or pili. Constructed by distinct assembly pathways resulting in diverse morphologies, fimbriae have been described to mediate functions including adhesion, motility, and DNA transfer. As these structures can represent major diversifying elements among Escherichia and Salmonella isolates, multiple fimbrial classification schemes have been proposed and a number of mechanistic insights into fimbrial assembly and function have been made. Herein we describe the classifications and biochemistry of fimbriae assembled by the chaperone/usher, curli, and type IV pathways.
Jeri pioneered studies of plant colonization mechanisms by the plant symbiont S. enterica1-3. Her genetic screen to identify S. enterica root attachment factors found that the majority of such genes were among those of putative or unknown function. Based on this work, the first human pathogen plant adhesin, thin aggregative fimbriae (curli in E. coli), was discovered1. The finding of active colonization mechanisms proved that these pathogens of humans cannot be simply washed from the surface of plants. This discovery was the first S. enterica regulator recognized as important outside an animal host (AgfD). The Barak lab dissected the role of each plant colonization factor regulated by AgfD: thin aggregative fimbriae, cellulose, and O-antigen capsule2. Thin aggregative fimbriae and cellulose production were the first plant colonization factors that were shown to differentiate S. enterica from E. coli. It was known that the E. coli O157:H7 curli and cellulose regulator was defective4; however, the ...
This study investigated the role of P fimbriae in colonization of Escherichia coli, host response, and bacterial persistence in humans. Human volunteers were inoculated intravesically with the nonadherent ABU isolate E. coli 83972 and with P fimbriated transformants of the same strain. During the following 24 h all urine samples, and thereafter daily samples, were collected for urine culture, analysis of neutrophil numbers, and cytokine concentrations (IL-6 and IL-8). The P fimbriated transformants showed enhanced bacterial colonization in comparison to E. coli 83972 and lowered the bacterial numbers needed for persistent bacteriuria. The P fimbriated transformants also lowered the bacterial numbers needed for a significant neutrophil and cytokine host response. We conclude that P fimbriae enhance bacterial colonization and trigger the host response in the human urinary tract ...
This protein is required for the assembly of the type IV fimbria in Pseudomonas aeruginosa responsible for twitching motility, and for a similar pilus-like structure in Synechocystis. It is also found in species such as Deinococcus described as having natural transformation (for which a type IV pilus-like structure is proposed) but not fimbria ...
The fimbriae of the uterine tube, also known as fimbriae tubae, are small, fingerlike projections at the end of the fallopian tubes, through which eggs move from the ovaries to the uterus. The fimbriae are connected to the ovary.
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate the periodic inversion of a 300bp DNA segment that harbors the promoter for the fimbrial structural gene, FimA. FimB switches FimA on.
Shop F7-2 fimbrial protein ELISA Kit, Recombinant Protein and F7-2 fimbrial protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
AbstractNeisseria gonorrheae bacteria are the causative agent of the second most common sexually transmitted infection in the world. The bacteria move on a surface by means of twitching motility. Their movement is mediated by multiple long and flexible filaments, called type IV pili, that extend from the cell body, attach to the surface, and retract, thus generating a pulling force. Moving cells also use pili to aggregate and form microcolonies. However, the mechanism by which the pili surrounding the cell body work together to propel bacteria remains unclear. Understanding this process will help describe the motility of N. gonorrheae bacteria, and thus the dissemination of the disease which they cause. In this article we track individual twitching cells and observe that their trajectories consist of alternating moving and pausing intervals, while the cell body is preferably oriented with its wide side toward the direction of motion. Based on these data, we propose a model for the collective ...
bacteria fimD protein: fimD gene necessary for surface localization of type 1 fimbriae; amino acid sequence given in first source
[IMG] Waziri wa Nchi Ofisi ya Makamu wa Pili Mohammed Aboud Mohammed Abood apinga urais wa kupokezana Waziri wa Nchi Ofisi ya Makamu wa Pili...
Raios dorsais (total): 9; Vértebras: 35 - 37. Vomero-palatine organ with only 5 pairs of fimbriate lamella; lamellae comparatively broad, each with up to 20 short fimbriae. Body dull white or silvery overall; fins dusky; colorless; humeral mark present, otherwise no distinctive markings. ...
Habari za j pili wana jf, Jana mwenzenu yamenikuta makubwa,baada ya uchovu wa kutwa nzima hatimae kidume nikaona nilale mapema coz kiubarid kilikuwa...
K02650 pilA; type IV pilus assembly protein PilA K02650 pilA; type IV pilus assembly protein PilA K02650 pilA; type IV pilus assembly protein PilA K02652 pilB; type IV pilus assembly protein PilB K02653 pilC; type IV pilus assembly protein PilC K02654 pilD; leader peptidase (prepilin peptidase) / N-methyltransferase [EC:3.4.23.43 2.1.1.-] K02654 pilD; leader peptidase (prepilin peptidase) / N-methyltransferase [EC:3.4.23.43 2.1.1.-] K02655 pilE; type IV pilus assembly protein PilE K02655 pilE; type IV pilus assembly protein PilE K02656 pilF; type IV pilus assembly protein PilF K02657 pilG; twitching motility two-component system response regulator PilG K02658 pilH; twitching motility two-component system response regulator PilH K02659 pilI; twitching motility protein PilI K02660 pilJ; twitching motility protein PilJ K02662 pilM; type IV pilus assembly protein PilM K02663 pilN; type IV pilus assembly protein PilN K02664 pilO; type IV pilus assembly protein PilO K02665 pilP; type IV pilus assembly ...
The fimbrial subunit gene from the benign type BBacteroides nodosus isolate AC/6 was cloned into theSphI site of the multicopy vector plasmid pUC19. FiveEscherichia coli recombinants that were positive in a colony immunoassay were shown, by Western transfer analysis, to produce an immunologically cross-reacting protein of identical molecular size to fimbrial subunits prepared fromB. nodosus AC/6. Restriction endonuclease analysis showed that 4 of the recombinant plasmids carried a 6.7 kbSphI fragment. Recloning experiments showed that the fimbrial subunit gene was located within a 2.5 kbEcoRI-SphI fragment and that there was aPstI site located within the structural gene or its regulatory region. These recombinant clones will prove useful for the construction of a multivalent recombinant vaccine for the control of ovine footrot.. ...
The ability to inhabit the different niches during an ascending urinary tract infection and cause particular pathologies at each site resides largely in the island genes specific to uropathogenic E. coli. The CFT073 genome sequence has revealed many possible factors that may contribute to colonization of the urinary tract tissues and the disease. The most important examples are mentioned here.. Surface structures known as fimbriae or pili mediate specificity for and attachment to host cells, an essential event for host colonization. We found genes encoding 12 distinct, putative fimbriae in the genome of CFT073, 10 fimbriae of the chaperone-usher family, and two type IV pili. Two pap operons (pylonephritis-associated pilus) encode P fimbriae with PapGII adhesins (17), located in islands at pheV and pheU. These are specific to uropathogens but are not the sole adhesins in CFT073 that are important for virulence. The foc operon encoding F1C fimbria and a chaperone-usher family operon with two ...
p,Bacteria have evolved a wide range of sensing systems to appropriately respond to environmental signals. Here we demonstrate that the opportunistic pathogen Pseudomonas aeruginosa detects contact with surfaces on short timescales using the mechanical activity of its type IV pili, a major surface adhesin. This signal transduction mechanism requires attachment of type IV pili to a solid surface, followed by pilus retraction and signal transduction through the Chp chemosensory system, a chemotaxis-like sensory system that regulates cAMP production and transcription of hundreds of genes, including key virulence factors. Like other chemotaxis pathways, pili-mediated surface sensing results in a transient response amplified by a positive feedback that increases type IV pili activity, thereby promoting long-term surface attachment that can stimulate additional virulence and biofilm-inducing pathways. The methyl-accepting chemotaxis protein-like chemosensor PilJ directly interacts with the major pilin ...
The ability to adhere onto surfaces is of very high importance for microorganisms, enabling them to stay in a favourable habitat for life. In the case of Bacteria cell surface organelles called fimbriae/pili have been shown to be used for adhesion; corresponding cell surface appendages of Archaea have not yet been defined. The first detailed characterization of archaeal fimbriae, namely those of Methanothermobacter thermoautotrophicus, allowed us to identify mth60 as the main structural fimbrin gene. Recombinant expression of mth60 in Escherichia coli was used to generate sufficient amounts of Mth60 to induce antibodies in rabbits. The antiserum reacted specifically with the 16 kDa fimbrial glycoprotein and could specifically detach adhering M. thermoautotrophicus cells from various surfaces. In addition we proved that cells adhering to solid surfaces - organic and inorganic ones - express many more fimbriae than cells growing in liquid cultures. The Mth60 fimbriae therefore are used by M. ...
Nanowires that transfer electrons to extracellular acceptors are important in organic matter degradation and nutrient cycling in the environment. Geobacter pili of the group of Type IV pilus are regarded as nanowire-like biological structures. However, determination of the structure of pili remains challenging due to the insolubility of monomers, presence of surface appendages, heterogeneity of the assembly, and low-resolution of electron microscopy techniques. Our previous study provided a method to predict structures for Type IV pili. In this work, we improved on our previous method using molecular dynamics simulations to optimize structures of Neisseria gonorrhoeae (GC), Neisseria meningitidis and Geobacter uraniireducens pilus. Comparison between the predicted structures for GC and Neisseria meningitidis pilus and their native structures revealed that proposed method could predict Type IV pilus successfully. According to the predicted structures, the structural basis for conductivity in G
The production of fimbrial adhesins K99 and F41 by enterotoxigenic Escherichia coli has been measured in steady-state chemostat experiments at various specific growth rates (microseconds) and in a recycling fermentor across a range of mu values falling to less than 0.004 h-1. It has been demonstrated that the production of K99 and F41 fimbriae is correlated with mu both in aerobic and anaerobic chemostat experiments. A significant production of fimbriae was only detected at mu values higher than 0.2 h-1. This behavior was further examined by culturing the bacteria in a recycling fermentor with complete biomass retention. It could be shown that the production of K99 and F41 fimbriae only occurred during balanced growth, with a high biomass yield at mu values higher than 0.04 h-1 corresponding to mass doubling times (td) of less than 17 h. The production of both fimbriae halted during balanced growth with a lower biomass yield (at mu values between 0.012 and 0.04 h-1 corresponding to td values ...
Fimbriae of Uterine Tube, 978-620-0-08853-6, Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In the female reproductive system, the fimbria (plural, fimbriae) is a fringe of tissue around the ostium of the Fallopian tube, in the direction of the ovary. An ovary is not directly connected to its adjacent Fallopian tube. When ovulation is about to occur, the sex hormones activate the fimbriae, causing it to swell with blood and hit the ovary in a gentle, sweeping motion. An oocyte is released from the ovary into the peritoneal cavity and the cilia of the fimbriae sweep the ovum into the Fallopian tube.
The human urinary tract is one of the most common sites of bacterial infection and most of them are caused by uropathogenic Escherichia coli (UPEC). During the infection, UPEC adhere to host epithelial cells by using specific extracellular adhesive organelles termed pili. Despite a wealth of other structural and biochemical information, little is known about how pilus formation is orchestrated at the bacterial cell surface. We present here a crystal structure capturing the pilus assembly platform in the act of secreting its cognate substrate. This new breakthrough provides new insights into the molecular details of pilus assembly.. Uropathogenic Escherichia coli (UPEC) attach specifically to human bladder cells using surface structures known as type 1 pili. These are assembled by the so-called chaperone-usher (CU) pathway, one of the best-characterised secretion systems in Gram-negative bacteria [1]. Type 1 pili consist of many individual subunits that are polymerised at the outer membrane by a ...
Les fimbriae sont des structures protéiques extracellulaires retrouvées chez une vaste diversité de bactéries. Ces structures ont fait lobjet de nombreuses études et sont maintenant reconnus pour leur implication dans ladhésion et linvasion aux cellules eucaryotes, mais aussi dans la production de biofilms. Ils sont groupés selon leur voie de sécrétion. Certains utilisent une machinerie spécifique et individuelle, cest le cas des pili de type IV, tandis que dautres utilisent la voie de sécrétion générale suivit dune voie spécifique telle que la voie du chaperon-placier (« Chaperon Usher Pathway ») (fimbriae CUP) ou la voie de nucléation précipitation (« nucleation precipitation pathway ») (Curli). Malgré toutes les connaissances actuelles concernant les fimbriae, très peu dinformations sont disponibles quant aux fimbriae de Salmonella enterica sérovar Typhi (S. Typhi). Ce pathogène unique à lhomme est lagent étiologique de la fièvre typhoïde. Puisque les ...
TY - JOUR. T1 - Force-dependent polymorphism in type IV pili reveals hidden epitopes. AU - Biais, Nicolas. AU - Higashi, Dustin L.. AU - Brujić, Jasna. AU - So, Magdalene. AU - Sheetz, Michael P.. PY - 2010/6/22. Y1 - 2010/6/22. N2 - Through evolution, nature has produced exquisite nanometric structures, with features unrealized in the most advanced manmade devices. Type IV pili (Tfp) represent such a structure: 6-nmwide retractable filamentous appendages found in many bacteria, including human pathogens. Whereas the structure of Neisseria gonorrhoeae Tfp has been defined by conventional structural techniques, it remains difficult to explain the wide spectrum of functions associated with Tfp. Here we uncover a previously undescribed force-induced quaternary structure of the N. gonorrhoeae Tfp. By using a combination of optical and magnetic tweezers, atomic force microscopy, and molecular combing to apply forces on purified Tfp, we demonstrate that Tfp subjected to approximately 100 pN of force ...
Sci Rep. 2021 Apr 15;11(1):8220. doi: 10.1038/s41598-021-87428-z.. ABSTRACT. Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the piliated isolates, in relation to antimicrobial susceptibility, serotypes, and genotypes at a major tertiary hospital on the east coast of Peninsular Malaysia. A total of 100 clinical isolates collected between September 2017 and December 2019 were subjected to serotyping, antimicrobial susceptibility test, and detection of pneumococcal virulence and pilus genes. Multilocus sequence typing (MLST) and phylogenetic analysis were performed only for piliated strains. The most frequent serotypes were 14 (17%), 6A/B (16%), 23F (12%), 19A (11%), and 19F (11%). The majority of isolates were resistant to erythromycin (42%), tetracycline ...
Summary Strains of Escherichia coli producing type-1 fimbriae, associated with mannose-sensitive haemagglutinin (MSHA), or three antigenically different kinds of MRE fimbriae, associated with mannose-resistant and eluting haemagglutinins (MREHAs), adhered poorly to HEp2 epithelial cells in an in-vitro adhesion model previously used to demonstrate the importance of motility and type-1 fimbriae for the attachment of strains of Salmonella typhimurium to HEp2 cells. Strains of E. coli producing narrow-spectrum MREHA, agglutinating human erythrocytes only of 14 red-cell species tested, adhered well to HEp2 cells, particularly so when bacteria produced MSHA (and type-1 fimbriae) along with the narrow-spectrum man-only MREHA. These findings are discussed with regard to recent observations suggesting that narrow-spectrum man-only MREHA in E. coli may be associated with fine, fibrillar appendages 2-nm wide.
Many bacterial pathogens interfere with cellular functions including phagocytosis and barrier integrity. The human pathogen Neissieria gonorrhoeae generates grappling hooks for adhesion, spreading, and induction of signal cascades that lead to formation cortical plaques containing f-actin and ezrin. It is unclear whether high mechanical forces generated by type IV pili (T4P) are a direct signal that leads to cytoskeletal rearrangements and at which time scale the cytoskeletal response occurs. Here we used laser tweezers to mimic type IV pilus mediated force generation by T4P-coated beads on the order of 100pN. We found that actin-EGFP and ezrin-EGFP accumulated below pilus-coated beads when force was applied. Within 2 min, accumulation significantly exceeded controls without force or without pili, demonstrating that T4P-generated force rapidly induces accumulation of plaque proteins. This finding adds mechanical force to the many strategies by which bacteria modulate the host cell cytoskeleton.
The yqiC gene of Salmonella enterica serovar Typhimurium (S. Typhimurium) regulates bacterial growth at different temperatures and mice survival after infection. However, the role of yqiC in bacterial colonization and host immunity remains unknown. We infected human LS174T, Caco-2, HeLa, and THP-1 cells with S. Typhimurium wild-type SL1344, its yqiC mutant, and its complemented strain. Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. Postinfection production of interleukin-8 and human β-defensin-3 in LS174T cells significantly reduced because of yqiC deleted in S. Typhimurium. The phenotype of yqiC mutant exhibited few and short flagella, fimbriae on the cell surface, enhanced biofilm formation, upregulated type-1 fimbriae expression, and reduced bacterial motility. Type-1 fimbriae, flagella, SPI-1, and SPI-2 gene expression was quantified using real-time PCR. The data show that deletion of yqiC upregulated fimA and fimZ expression and
A new locus required for type 4 pilus biogenesis by Pseudomonas aeruginosa has been identified. A pilE mutant, designated MJ-6, was broadly resistant to pili-specific phages and unable to translocate across solid surfaces by the pilus-dependent mechanism of twitching motility (Twt-). Immunoblot anal …
森 修二 , 広瀬 公治 , 大井戸 真理 , 室 三之 , 磯貝 恵美子 , 中島 啓介 , 上田 五男 , 小鷲 悠典 日本歯周病学会会誌 40(1), 58-65, 1998-03-28 CiNii PDF - オープンアクセス J-STAGE 医中誌Web 参考文献22件 被引用文献2件 ...
Bacterial attachment to the host surfaces is the first and key step in colonization, which may harm or benefit the host based on the natureof host-microbial relationship. Bacteria often assemble and use hair-like organelles known as pili or fimbriae on their cell surface to quickly and effectively mediate attachment. This initial recognition between the bacteria and host through surface molecules determines the tissue tropism and defines host range. Combating bacterial infections by targeting pili or pili-mediated interactions is recognized as a promising approach that may help overcome their ever-increasing repertoires of resistance mechanisms. This requires knowledge of how bacteria assemble pili and use them for adherence. In pathogens, the pili and their components have been studied and also recognized as virulence factors and good vaccine candidates because of their key role in pathogenesis and immunogenic properties.. Interestingly, the beneficial or probiotic bacteria that benefit us also ...
Archaeal flagellum. The flagellum of Archaea is a long hair-like cell surface appendage made of polymerized flagellin with an attached hook. This rotating structure with switches propels the cell through a liquid medium. The archaeal flagellum is distinct from its bacterial equivalent in terms of architecture, composition and mechanism of assembly. Thinner (10-15 nm) compared to the bacterial flagellum (18-24 nm), it is usually composed of several types of flagellins and is glycosylated. The archeal flagellum is considered as a type IV pilus-like structure.. Category: Cellular component ...
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Among the minority of piliated cocci in the brain showing a division septum, we could distinguish bacteria in the early and late stages of cell division by high-resolution microscopy (Supplemental Figure 12, A and B). Even though most single cocci did not express DivIVA in the brain, bacteria caught in the division process were DivIVA positive. In particular, at the early stages of cell division, when bacteria had a round shape, with the formation of a division septum, DivIVA was expressed at the poles of the cells (Supplemental Figure 12A). During cell division, the bacterial cell was more elongated and more closely resembled a diplococcus with 2 nearly formed daughter cells. At this stage, the DivIVA signal was severely decreased (Supplemental Figure 12B). These findings strongly suggest that the DivIVA protein is not always absent in piliated bacteria during brain invasion but is instead present for only a limited time while single cocci are dividing. This very limited expression of DivIVA ...
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Bacterial adhesion to other bacteria, to eukaryotic cells, and to extracellular matrix proteins is frequently mediated by cell surface-associated polymers (fimbriae) consisting of one or more subunit proteins. We have found that polymerization of curlin to fimbriae-like structures (curli) on the surface of Escherichia coli markedly differs from the prevailing model for fimbrial assembly in that it occurs extracellularly through a self-assembly process depending on a specific nucleator protein. The cell surface-bound nucleator primes the polymerization of curlin secreted by the nucleator-presenting cell or by adjacent cells. The addition of monomers to the growing filament seems to be driven by mass action and guided only by the diffusion gradient between the source of secreted monomer and the surface of monomer condensation.. ...
DNA hybridization experiments demonstrated that the gene clusters encoding the F8 fimbriae (fei) as well as the type I fimbriae (pi/) exist in a single copy on the chromosome of E. coli 018:K5 strain 2980. In conjugation experiments with appropriate donors, the chromosomal site of these gene clusters was determined. The pil genes were mapped close to the gene clusters thr and Jeu controlling the biosynthesis of threonine and leucine, respectively. The fei genes were found to be located close to the galactose operon (gal) between the position 17 and 21 of the E. coli chromosomallinkage map ...
Papež Jan Pavel II. se již poněkolikáté vrátil do nemocnice, vážně nemocen. I pro nekatolíky, jako jsem já, jde o příležitost k zamyšlení, o co svět přijde, až papežovo tělo nakonec podlehne svým neduhům. Obraz, který vyvstane, je směsicí barev. Pro ty z nás, kdo považují pád komunismu z roku 1989 za zásadní událost dějin dvacátého století, je papež Jan Pavel hrdina. V Polsku byl ohniskem všech aktivit občanské společnosti. Zatímco v ostatních zemích, nejsilněji v Rumunsku, ale i v tehdejším Československu a v Maďarsku, vedle komunismu bylo vakuum nebo přinejlepším několik izolovaných organizací občanské společnosti, Polsko mělo alternativní zdroj legitimity. Tehdejší krakovský kardinál Karol Wojtyla byl jeho nejpůsobivějším představitelem, před svým zvolením i po něm. Zvolení kardinála Wojtyly papežem tak svým významem přesahovalo hranice církve. On sám vlastně ztotožňování své církve s občanskou ...
I am standing the flexibility of the bacterial pili and i was wondering if i could substantiate that in order for a bacterium to colonize the nasopharynx epithelia (mucus) should be more flexible than colonizing the lower parts of the respiratory tracts ...
Health,...Researchers discover key clues to bacterias structure ...WEDNESDAY June 10 (HealthDay News) -- Montezumas revenge also known...Now researchers have figured out how the bacteria responsible for the...The bacteria use surface pili or fimbriae to attach or bind to t...,Finding,May,Lead,to,Vaccine,for,Travelers,Diarrhea,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
A new study appearing in the journal Science shows that bacteria need the resistance to pilus retraction that occurs upon contact with a surface in order to sense surfaces and excrete the glue that makes them firmly adhere.
Finally got my bfp!(: last period was September 4th. I took a test last week an got a bfn so I had a longer cycle then I thought. Yay(:
Hi Ladies, I cant believe it .. finally got my BFP this morning..am in total shock ..the positive came up instantly! We have been trying but not tryi...
Michaelis, W. , Seifert, R. , Nauhaus, K. , Treude, T. , Thiel, V. , Blumenberg, M. , Knittel, K. , Gieseke, A. , Peterknecht, K. , Pape, T. , Boetius, A. , Amann, R. , Jorgensen, B. B. , Widdel, F. , Peckmann, J. , Pimenov, N. V. and Gulin, M. B. (2002 ...
马为民博士,教授。主要研究领域蓝藻类囊体膜上光合蛋白的网络调控。研究工作简介一直从事蓝藻光合作用等方面的研究。主持和参加国家自然科学基金各一项。发表学术论文19篇,其中SCI论文12篇。近年来承担的科研项目一种新型NADPH脱氢酶超分子复合体生理功能的研究。2008.01―2010.12,国家自然科学基金。(主持)一种新型蛋白CupB在蓝藻CO2浓缩中的调控作用。2005.01―2007.12,国家自然科学基金。(参加)近三年发表的SCI论文1.MaW,DengYandMiH(2007)RedoxofplastoquinonepoolregulatestheexpressionandactivityofNADPHdehydrogenasesupercomplexinSynechocystissp.strainPCC6803.CurrMicrobiol(Onlinepublication)2.MaW,ChenL,WeiLandWangQ(2007)ExcitationenergytransferbetweenphotosystemsinthecyanobacteriumSynechocy
May testing thread: 1- snameoy - BFP! , peanutoven 2- kitzycat 3- lilbubs4us 4- bubbles21 , Fauvette - BFP! 5- 6- 2weekhate 7- 8- Lizzy083 - page 75
M rgised taimed Autor: Pille Kolk Rosella | Pilt Papagoimaja galeriist Eess na: Andmeid m rgiste taimede kohta olen kogunud erinevatelt veebilehek lgedelt,
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Fimbriae. A fimbria (plural fimbriae also known as a pilus, plural pili) is a short, thin, hair-like filament found on the ... Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus ... Fimbriae are formed of a protein called pilin (antigenic) and are responsible for the attachment of bacteria to specific ... Michie KA, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annual Review of Biochemistry. 75: 467-92. doi: ...
Fimbria. A fimbria also known as a pilus is a short, thin, hair-like filament found on the surface of bacteria. Fimbriae, or ... Michie KA, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annual Review of Biochemistry. 75: 467-92. doi: ... The bacterial flagellum stretches from cytoplasm through the cell membrane(s) and extrudes through the cell wall. They are long ... Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus ...
Bacterial nanowires Flagellum Sortase P fimbriae "pilus" at Dorland's Medical Dictionary Brinton, Charles (1954). " ... The terms pilus and fimbria (Latin for 'fringe'; plural: fimbriae) can be used interchangeably, although some researchers ... Fimbriae are either located at the poles of a cell or are evenly spread over its entire surface. Mutant bacteria that lack ... Bacterial type IV pili are similar in structure to the component flagellins of archaella (archaeal flagella), and both are ...
Bacterial flagella are thicker than archaella, and the bacterial filament has a large enough hollow "tube" inside that the ... Fimbriae and pili are also thin appendages, but have different functions and are usually smaller. Three types of flagella have ... The similarities between bacterial flagella and bacterial secretory system structures and proteins provide scientific evidence ... They provide two of several kinds of bacterial motility. Archaeal flagella (archaella) are superficially similar to bacterial ...
Length changes in bacterial SSRs can affect fimbriae formation in Haemophilus influenzae, by altering promoter spacing. ...
Short fimbriae (Mfa1), also known as minor fimbriae, have distinct roles from long fimbriae and are characterized to be ... List of bacterial vaginosis microbiota Naito M, Hirakawa H, Yamashita A, Ohara N, Shoji M, Yukitake H, et al. (August 2008). " ... P. gingivalis has long fimbriae, short fimbriae, and accessory components, each of which have distinct functions. Long fimbriae ... Short fimbriae are involved in cell-cell adhesion with other dental commensals. It was found to coadhere and develop biofilm in ...
Although not all bacteria have pili or fimbriae, bacterial pathogens often use their fimbriae to attach to host cells. In Gram- ... Genetic transformation is the process by which a recipient bacterial cell takes up DNA from a neighboring cell and integrates ... Kang HJ, Coulibaly F, Clow F, Proft T, Baker EN (2007). "Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus ...
Most members of Enterobacteriaceae have peritrichous, type I fimbriae involved in the adhesion of the bacterial cells to their ...
... fimbriae) to receptor. The bacterial adhesion reduction is reached by Met I ad concentration similar to the plasmatic peak ... Antiadhesive activity Erdosteine is able to interfere with bacterial adhesion. In fact, Met I can affect the integrity of the ... Erdosteine showed in vivo and in vitro synergistic activity with antibiotics, against bacterial adhesiveness, in patients with ... the opening of this bond can induce a morphological change that interferes with the binding of bacterial adhesin ( ...
"Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro ... The smaller size and single domain make these antibodies easier to transform into bacterial cells for bulk production, making ...
Swarming motility is the coordinated translocation of a bacterial population driven by flagellar rotation in film or on fluid ... These include adherence due to the presence of fimbriae or afimbrial adhesins, invasiveness, swarming phenomenon, hemolytic ... Second German-Polish-Russian Meeting on Bacterial Carbohydrates, Moscow, September 10-12, 2002. Zych K, Kowalczyk M, Knirel YA ... Type strain of Proteus penneri at BacDive - the Bacterial Diversity Metadatabase Biology portal. ...
Prokaryotes have adhesion molecules on their cell surface termed bacterial adhesins, apart from using its pili (fimbriae) and ... Pizarro-Cerdá, Javier; Cossart, Pascale (2006). "Bacterial Adhesion and Entry into Host Cells". Cell. 124 (4): 715-727. doi: ... Ofek, Itzhak; Hasty, David L; Sharon, Nathan (2003). "Anti-adhesion therapy of bacterial diseases: prospects and problems". ... Klemm, Per; Schembri, Mark A. (2000). "Bacterial adhesins: function and structure". International Journal of Medical ...
He received a PhD from the University of Wisconsin for his work on bacterial pili and fimbriae involved in host-pathogen ...
A fimbria (plural fimbriae also known as a pilus, plural pili) is a short, thin, hair-like filament found on the surface of ... The bacterial flagellum stretches from cytoplasm through the cell membrane(s) and extrudes through the cell wall. They are long ... Fimbriae are formed of a protein called pilin (antigenic) and are responsible for the attachment of bacteria to specific ... Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus ...
pilus) are cellular appendages, slightly larger than fimbriae, that can transfer genetic material between bacterial cells in a ... There are typically 40 million bacterial cells in a gram of soil and a million bacterial cells in a millilitre of fresh water. ... Fimbriae are believed to be involved in attachment to solid surfaces or to other cells, and are essential for the virulence of ... Bacterial cells are about one-tenth the size of eukaryotic cells and are typically 0.5-5.0 micrometres in length. However, a ...
Bacterial resistance against taurolidine has never been observed in various studies. The use of a taurolidine lock solution may ... Catheter lock solution: Taurolidine decreases the adherence of bacteria and fungi to host cells by destructing the fimbriae and ... Side effects and the induction of bacterial resistance is uncommon. It is also being studied as a treatment for cancer. It is ... These labile N-methylol derivatives of taurultam and taurinamide react with the bacterial cell-wall resulting in lysis of the ...
In this context, c-di-GMP functions as a bacterial secondary messenger, enhancing the production of extracellular cellulose and ... fimbria) and producing ECM polysaccharides (cellulose). Following a promoter-lacZ fusion assay of CsgD binding to specific ... CsgD is a transcription and response regulator protein referenced to as the master modulator of bacterial biofilm development. ... encoding for curli fimbriae, and adrA, encoding for the synthesis of cyclic diguanylate. ...
Bacterial preys will then collapse, as they do for example when treated with Β-lactam antibiotics. Filloux carried on studying ... or fimbriae, he called Cup (Ref Vallet et al., PNAS, 2001). Working with Stephen Lory at Harvard Medical School, Filloux ... Filloux used bacterial genetics to identify a series of molecular determinants involved in the film formation process, notably ... It was known that high levels of c-di-GMP in bacterial cells turns on biofilm development, and Filloux showed that ...
The persistence of B. longum in the gut is attributed to the glycoprotein-binding fimbriae structures and bacterial ... the Bacterial Diversity Metadatabase New Strain of Bifidobacterium May Help Improve Metabolic and Mental Health. On: sci-news. ...
... fimbriae, bacterial MeSH A11.284.180.290 - flagella MeSH A11.284.180.290.835 - sperm tail MeSH A11.284.180.565 - microvilli ... bacterial MeSH A11.284.187.190.170 - chromosomes, artificial, bacterial MeSH A11.284.187.360 - chromosomes, fungal MeSH A11.284 ... bacterial chromatophores MeSH A11.284.430.214.190.875.117 - cell nucleus MeSH A11.284.430.214.190.875.117.550 - macronucleus ... bacterial MeSH A11.284.187.178.190 - chromosomes, artificial, mammalian MeSH A11.284.187.178.190.117 - chromosomes, artificial ...
... pili or fimbriae (also fimbria, from the Latin word for "fringe") can be trapped by Tamm-Horsfall protein via its mannose- ... A role in bacterial binding and sequestration is suggested by studies showing that Escherichia coli which express MS (mannose- ...
A variety of bacterial traits and specific genes contribute to this process, but only a few have been identified. These include ... motility, chemotaxis to seed and root exudates, production of pili or fimbriae, production of specific cell surface components ... "Genetic approaches for analysis and manipulation of rhizosphere colonization by bacterial biocontrol agents". In Boland, Greg J ...
Bacterial gliding is a type of gliding motility that can also use pili for propulsion. The speed of gliding varies between ... and fimbriae. Gliding allows microorganisms to travel along the surface of low aqueous films. The mechanisms of this motility ... Bacterial gliding is a process of motility whereby a bacterium can move under its own power. Generally, the process occurs ... McBride, M. . (2001). "Bacterial gliding motility: multiple mechanisms for cell movement over surfaces". Annual Review of ...
This stimulates antibody production against the bacterial pilli to prevent their attachment and invasion of the conjunctiva. ... M. bovis uses several different serotyped fimbriae as virulence factors, consequently pharmaceutical companies have exploited ... and oversized or incorrectly placed ear tags may cause mechanical damage to the eye and facilitate bacterial colonization. The ...
... in the juice's ability to block bacterial adhesion, demonstrated by the compressing the fimbria of E. coli bacteria in the ...
It has polar fimbriae and is the causative agent of ovine foot rot as well as interdigital dermatitis. It is the lone species ... Environmental management and treatment of infected is required to prevent further bacterial spread. D. nodosus is a rod shaped ... It has polar fimbriae epitomes that separate the bacteria into multiple serogroups. D. nodosus, along with Fusobacterium ... D. nodosus releases protease enzymes, it is the number of protease enzymes, type of protease enzymes and the type IV fimbriae ...
Schembri MA, Blom J, Krogfelt KA, Klemm P (August 2005). "Capsule and fimbria interaction in Klebsiella pneumoniae". Infection ... Bacterial cell structure Quellung reaction, a method to visualize capsule under a microscope Peterson JW (1996). Bacterial ... The bacterial capsule is a large structure common to many bacteria. It is a polysaccharide layer that lies outside the cell ... When viewed, bacterial capsules appear as a bright halo around the cell on a dark background. The capsule is considered a ...
There are many fimbriae on mainly Gram-negative bacteria, so displaying proteins on fimbriae is advantageous over some other ... Bacterial display systems were first introduced by Freudl et al. and Charbit et al. in 1986, when they used bacterial surface ... Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro ... OMPs are common scaffolds for bacterial display. Proteins can also be displayed on the bacterial cell surface through the use ...
The typical structure of a bacterial adhesion is that of a fimbria or pilus. The bacterial adhesion consists primarily of an ... However, bacterial adhesins do not serve as a sort of universal bacterial Velcro. Rather, they act as specific surface ... Adhesion and bacterial adhesins are also a potential target for prophylaxis or treatment of bacterial infections. Bacteria are ... During the bacterial lifespan, a bacterium is subjected to frequent shear-forces. In the crudest sense, bacterial adhesins ...
Abel-Santos, E (editor) (2012). Bacterial Spores: Current Research and Applications. Caister Academic Press. ISBN 978-1-908230- ... Further information: Bacterial morphological plasticity. Under conditions of starvation, especially the lack of carbon and ... While the rest of a bacterial cell may stain, the endospore is left colourless. To combat this, a special stain technique ... Bacterial endospores are resistant to antibiotics, most disinfectants, and physical agents such as radiation, boiling, and ...
This includes parts (coats, capsules, cell walls, flagella, fimbriae, and toxins) of bacteria, viruses, and other ... or because of viral or intracellular bacterial infection. The fragments are then presented on the cell surface in the complex ... named the hypothetical substances halfway between bacterial constituents and antibodies "substances immunogenes ou antigenes" ( ...
"En Ladant, Daniel; Alouf, Joseph E.; Popoff, Michel R. The Comprehensive Sourcebook of Bacterial Protein Toxins. Academic Press ... Lindler LE, Tall BD (1993). "Yersinia pestis pH 6 antigen forms fimbriae and is induced by intracellular association with ... "Identification of murine T cells reactive with the bacterial superantigen Yersinia pseudotuberculosis-derived mitogen (YPM) and ... "Bacterial resistance to complement killing mediated by the Ail protein of Yersinia enterocolitica". Proc. Natl. Acad. Sci. U.S ...
Tanging sa Bakteryang negatibo sa metodo ni Gram: Bacterial outer membrane (Porin, Lipopolysaccharide) · Periplasmic space ...
Fimbria (with 29. Fimbria ovarica). 30. Ovary. 31. Visceral pelvic peritoneum: 23. Perimetrium; 32. Broad ligament (with 33. ... Bacterial vaginosisEdit. Main article: Bacterial vaginosis. This is a vaginal infection in women. It differs from vaginitis in ... Bacterial vaginosis is polymicrobial, consisting of many bacteria species. The diagnosis for bacterial vaginosis is made if ...
For a bacterial cell to reproduce through binary fission, more than a million peptidoglycan subunits (NAM-NAG+oligopeptide) ... The peptidoglycan layer in the bacterial cell wall is a crystal lattice structure formed from linear chains of two alternating ... Some antibacterial drugs such as penicillin interfere with the production of peptidoglycan by binding to bacterial enzymes ... Peptidoglycan is also involved in binary fission during bacterial cell reproduction. The peptidoglycan layer is substantially ...
a b Bacterial phyla entry in LPSN [. Euzéby, J.P. (1997). "List of Bacterial Names with Standing in Nomenclature: a folder ... Branching order of bacterial phyla (Rappe and Giovanoni, 2004). *Branching order of bacterial phyla after ARB Silva Living Tree ... List of sequenced bacterial genomes. Footnotes[edit]. *^ Past editions of Brock Biology of Microorganisms have referred to the ... When bacterial nomenclature was controlled under the Botanical Code, the term division was used, but now that bacterial ...
Arrangement of Bacterial Flagella. Diakses pada 22 Juni 2011.. *^ Wenner M. 2007. Humans Carry More Bacterial Cells than Human ... Banyak bakteri memiliki struktur di luar sel lainnya seperti flagel dan fimbria yang digunakan untuk bergerak, melekat dan ... Online collaboration for bacterial taxonomy.. *PATRIC, a Bioinformatics Resource Center for bacterial pathogens, funded by ... Bacterial Chemotaxis Interactive Simulator - A web-app that uses several simple algorithms to simulate bacterial chemotaxis. ...
For example, while bacterial cell membranes are made from phosphoglycerides with ester bonds, archaean membranes are made of ... However, many bacterial species can transfer DNA between individual cells by a horizontal gene transfer process referred to as ... Their genome is usually a circular bacterial chromosome - a single loop of DNA, although they can also harbor small pieces of ... These plasmids can be transferred between cells through bacterial conjugation. Bacteria have an enclosing cell wall, which ...
Parts of a bacterial cell. *Bacterial Chemotaxis Interactive Simulator - A web-app that uses several simple algorithms to ... Tanging sa Bakteryang negatibo sa metodo ni Gram: Bacterial outer membrane (Porin, Lipopolysaccharide) · Periplasmic space ... Cabeen M, Jacobs-Wagner C (2005). "Bacterial cell shape". Nat Rev Microbiol. 3 (8): 601-10. doi:10.1038/nrmicro1205. PMID ... Bacterial Nomenclature Up-To-Date from DSMZ. *Genera of the domain Bacteria - list of Prokaryotic names with Standing in ...
It is part of a biopolymer in the bacterial cell wall, which is built from alternating units of GlcNAc and N-acetylmuramic acid ...
... 's primary role is to sequester free iron, and in doing so remove essential substrate required for bacterial growth ... Sojar HT, Hamada N, Genco RJ (1998). "Structures involved in the interaction of Porphyromonas gingivalis fimbriae and human ... Lactoferrin binds to lipopolysaccharide of bacterial walls, and the oxidized iron part of the lactoferrin oxidizes bacteria via ... Singh PK, Parsek MR, Greenberg EP, Welsh MJ (2002). "A component of innate immunity prevents bacterial biofilm development". ...
Length changes in bacterial SSRs can affect fimbriae formation in Haemophilus influenzae, by altering promoter spacing.[31] ...
Michie K, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annu Rev Biochem 75: 467-92. doi:10.1146/annurev. ... Fimbria (bacteriology) wikipedia https://en.wikipedia.org/wiki/Fimbria_(bacteriology) *↑ ಬ್ಯಾಕ್ಟೀರಿಯಾ ಕಾಂಜುಗೇಶನ್‌- ...
The cause can be viral or bacterial, however, E. coli is often involved. As MOS can bind E. coli (see Effects of MOS on the ... Different forms of mannose-type sugars interact differently with type-1-fimbriae. The form present in the cell wall of ... Different studies showed that salmonella can bind via type-1-fimbriae (finger-like projections) to mannose. The binding to ... As mentioned earlier MOS affects bacterial attachment in the intestinal tract. In controlled studies with chickens, a reduction ...
Fimbriae. A fimbria (plural fimbriae also known as a pilus, plural pili) is a short, thin, hair-like filament found on the ... Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus ... Fimbriae are formed of a protein called pilin (antigenic) and are responsible for the attachment of bacteria to specific ... Michie KA, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annual Review of Biochemistry. 75: 467-92. doi: ...
Most members of Enterobacteriaceae have peritrichous, type I fimbriae involved in the adhesion of the bacterial cells to their ...
Hockertz S. Augmentation of host defence against bacterial and fungal infections of mice pretreated with the non-pathogenic ... M and S fimbriae), no hemolysins, no serum resistance (i.e. no risk of sepsis), no uropathogenicity and no antibiotic- ... Mutaflor is a probiotic consisting of a viable non-pathogenic bacterial strain named Escherichia coli Nissle 1917. The ... is one of the best-examined and therapeutically relevant bacterial strains worldwide. The Mutaflor strain was isolated by ...
For a catch-bond, the force on the cell pulls the adhesive tip of a fimbria to close tighter on its target cell. As the ... Thomas WE, Trintchina E, Forero M, Vogel V, Sokurenko EV (June 2002). "Bacterial adhesion to target cells enhanced by shear ... Thomas WE, Trintchina E, Forero M, Vogel V, Sokurenko EV (June 2002). "Bacterial adhesion to target cells enhanced by shear ... People with LAD suffer from recurrent bacterial infections and impaired wound healing. Neutrophilia is a hallmark of LAD. ...
Prokaryotes have adhesion molecules on their cell surface termed bacterial adhesins, apart from using its pili (fimbriae) and ... Pizarro-Cerdá, Javier; Cossart, Pascale (2006). "Bacterial Adhesion and Entry into Host Cells". Cell. 124 (4): 715-727. doi: ... Klemm, Per; Schembri, Mark A. (2000). "Bacterial adhesins: function and structure". International Journal of Medical ... Ofek, Itzhak; Hasty, David L; Sharon, Nathan (2003). "Anti-adhesion therapy of bacterial diseases: prospects and problems". ...
There are different catabolite-specific EIIA in a single cell, even though different bacterial groups have specificities to ...
Tanging sa Bakteryang negatibo sa metodo ni Gram: Bacterial outer membrane (Porin, Lipopolysaccharide) · Periplasmic space ... Infectious diseases · Bacterial diseases: G+ (primarily A00-A79, 001-041, 080-109) ...
in the bacterial phylum Firmicutes". Int. J. Syst. Evol. Microbiol. 60 (Pt 6): 1271-9. doi:10.1099/ijs.0.013102-0. PMID ... Bacterial transformation[edit]. Transformation is one of three processes for horizontal gene transfer, in which exogenous ... A given bacteria's staining result, bacterial membrane organization, and lineage groupings do not always match up.[6][7][8][9] ... Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the gram-staining method of bacterial ...
Parts of a bacterial cell. *Bacterial Chemotaxis Interactive Simulator - A web-app that uses several simple algorithms to ... Fimbriae ni nyuzi nyororo za protini, zenye kipenyo cha nonimita 20-10 tu na urefu wa mikromita kadhaa. Huwa wamesambaa kwenye ... Young K (2006). "The selective value of bacterial shape". Microbiol Mol Biol Rev 70 (3): 660-703. doi:10.1128/MMBR.00001-06 . ... Cabeen M, Jacobs-Wagner C (2005). "Bacterial cell shape". Nat Rev Microbiol 3 (8): 601-10. doi:10.1038/nrmicro1205 . PMID ...
Principles of Bacterial Pathogenesis. Academic Press. pp. 619-674. ISBN 0-12-304220-8. Mattoo S, Cherry J (2005). "Molecular ... These include filamentous haemaglutinin, pertactin, fimbriae, and pertussis toxin (though expression of pertussis toxin is ... being a normal product of the breakdown of the bacterial cell wall. Other bacteria recycle this molecule back into the ...
... is a protein found in the immune system, and is a common defense against bacterial infections, which it is able to ... Sojar HT, Hamada N, Genco RJ (January 1998). "Structures involved in the interaction of Porphyromonas gingivalis fimbriae and ... Lactoferrin's primary role is to sequester free iron, and in doing so remove essential substrate required for bacterial growth. ... Singh PK, Parsek MR, Greenberg EP, Welsh MJ (May 2002). "A component of innate immunity prevents bacterial biofilm development ...
To differentiate any bacterial growth from other species a small amount of a bacterial colony is tested for oxidase, catalase ... fimbriae mediate attachment of the bacterium to the epithelial cells of the nasopharynx.[14][15] It infects the cell by ... 2009). Bacterial Polysaccharides: Current Innovations and Future Trends. Caister Academic. ISBN 978-1-904455-45-5.. ... It causes the only form of bacterial meningitis known to occur epidemically, mainly Africa and Asia. It occurs worldwide in ...
Fimbria[Redakto , Redakto nëpërmjet kodit]. Fimbria e njohur edhe si pilus është një fije e shkurtër, e hollë, si flokët, që ... Michie KA, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annual Review of Biochemistry. 75: 467-92. doi: ... Fimbria formohet nga një proteinë e quajtur pilin ( antigjenik ) dhe janë përgjegjës për ngjitjen e baktereve në receptorët ...
Bacterial capsule. *Slime layer. *S-layer. *Glycocalyx. *Pilus. *Fimbria. *Non-motile bacteria ...
P-fimbriae, bacterial adhesion, and pyelonephritis. Message subject: (Your Name) has forwarded a page to you from Archives of ...
Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation. ... Influence of Fimbriae on Bacterial Adhesion and Viscoelasticity and Correlations of the Two Properties with Biofilm Formation ... Capsular polysaccharides and fimbriae are two major components on a bacterial surface, which are critical for mediating cell- ... Wang, H, Wilksch, J, Chen, L, Tan, J, Strugnell, R and Gee, M 2016, Influence of Fimbriae on Bacterial Adhesion and ...
Purified fimbriae retained their ability to bind to erythrocytes in a mannose-inhibitable fashion and, in doing so, behaved ... Type 1 fimbriae have been purified from a Salmonella typhi strain of clinical origin. ... Fimbriae Proteins* * Fimbriae, Bacterial* / chemistry * Fimbriae, Bacterial* / immunology * Fimbriae, Bacterial* / ... Type 1 fimbriae have been purified from a Salmonella typhi strain of clinical origin. Purified fimbriae retained their ability ...
Fimbriae. A fimbria (plural fimbriae also known as a pilus, plural pili) is a short, thin, hair-like filament found on the ... Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus ... Fimbriae are formed of a protein called pilin (antigenic) and are responsible for the attachment of bacteria to specific ... Michie KA, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annual Review of Biochemistry. 75: 467-92. doi: ...
... fimbriae are adhesive surface organelles common to many Gram-negative bacteria. Escherichia coli genomes contain a large ... Fimbriae, Bacterial / genetics * Fimbriae, Bacterial / metabolism* * Genome, Bacterial * Molecular Chaperones / genetics* * ... A group of core-associated E. coli CU fimbriae were defined and include the Type 1, Yad, Yeh, Yfc, Mat, F9 and Ybg fimbriae. ... Chaperone-usher fimbriae of Escherichia coli PLoS One. 2013;8(1):e52835. doi: 10.1371/journal.pone.0052835. Epub 2013 Jan 30. ...
Fimbria. A fimbria also known as a pilus is a short, thin, hair-like filament found on the surface of bacteria. Fimbriae, or ... Michie KA, Löwe J (2006). "Dynamic filaments of the bacterial cytoskeleton". Annual Review of Biochemistry. 75: 467-92. doi: ... The bacterial flagellum stretches from cytoplasm through the cell membrane(s) and extrudes through the cell wall. They are long ... Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus ...
HomeBacteriologyBacterial Pili (Fimbriae): Characteristics, Types and Medical Importance. Bacterial Pili (Fimbriae): ... Fimbriae (pili) are shorter, straighter and more numerous than bacterial flagella and are composed by subunits of protein ... Bacterial Flagella: Structure, importance and examples of flagellated bacteria April 28, 2013 Acharya Tankeshwar Bacteriology, ... Medical Importance of Fimbriae or Pili. *Common pili (Adhesins): They mediate the attachment of bacteria to specific receptors ...
FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. These proteins mediate ... Ensembl bacterial and archaeal genome annotation project. More...EnsemblBacteriai. AAC77268; AAC77268; b4312. BAE78305; ... Type 1 fimbriae regulatory protein FimBAdd BLAST. 200. Proteomic databases. jPOST - Japan Proteome Standard Repository/Database ... "Inversion-independent phase variation of type 1 fimbriae in Escherichia coli.". McClain M.S., Blomfield I.C., Eberhardt K.J., ...
Western blotting.Bacterial cells from plates were resuspended to an optical density at 600 nm (OD600) of 1.0 in 50 mM sodium ... However, it was unclear whether the algR mutants failed to synthesize surface fimbriae or if the fimbriae were expressed but ... 4A) and Western analyses of sheared surface fimbriae (data not shown) showed that PAKalgR10 overproduces fimbriae on the cell ... Type IV fimbriae were detected using antipilin antiserum and are indicative of levels of surface fimbriae in these strains. ...
... of these strains express K88ac fimbriae (48). In addition, K88ac fimbriae, like other bacterial flagella or fimbriae, are ... Porcine brush border bacterial adherence assay to examine chimeric fimbriae in binding.Jejunal small intestine segments were ... Bacterial adherence inhibition by serum and fecal samples from rabbits immunized with chimeric K88ac fimbriae. Porcine ETEC ... K88ac fimbriae, like other bacterial adhesins, may also serve as platforms for developing epitope vaccines against other ...
Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species OGAWA T. ... Bacteroides gingivalis fimbriae stimulate production of thymocyte-activating factor by human gingival fibroblasts HANAZAWA S. ... Detection of Fimbriae and Fimbrial Antigens on the Oral Anaerobe Bacteroides gingivalis by Negative Staining and Serological ... Localization of Porphyromonas gingivalis-carrying Fimbriae in situ in Human Periodontal Pockets NOIRI Y. , LI L. , YOSHIMURA F ...
in The comprehensive sourcebook of bacterial protein toxins. eds Allouf J. E., Freer J. (Academic Press, London, England), pp 3 ... F1C-fimbriae (foc), and S-fimbria-related fimbriae (sfr), PCRs were established with primer pairs which are specific for sfaA I ... F1C-fimbriae (Foc); and S/F1C-related fimbriae (Sfr) (25, 46). The AC/I-fimbriae (Fac) which are expressed by avian-pathogenic ... 1988) Cloning and characterization of a new type of fimbriae (S/FlC related fimbria) expressed by an Escherichia coli O75:K1:H7 ...
Bacterial strains were grown on CFA agar with or without bile salts overnight at 37°C and suspended to 109 bacteria/ml in ... Purification of ETEC fimbriae.CFA/I and CS17 fimbriae were purified as previously described (16, 17). Briefly, bacteria were ... Our findings suggest that the minor subunits of class 5 fimbriae may be superior to whole fimbriae in inducing antiadhesive ... Fimbriae, p. 146-157. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. ...
Bacterial pathogenesis - Bacterial adhesion. - Salmonella and Escherichia coli fimbriae - Salmonella and adhesin vaccines - ... Our research is directed towards bacterial ligands that bind to specific host receptors and mediate bacterial colonization, ... Schifferli, Dieter M.: Histone H1 proteins act as receptors for the 987P fimbriae of enterotoxigenic Escherichia coli. Journal ... Galván Estela M, Chen Huaiqing, Schifferli Dieter M: The Psa fimbriae of Yersinia pestis interact with phosphatidylcholine on ...
fimbriae. What component of the bacterial cell wall gives it rigidity?. peptidoglycan. ... The gelatinous contents within the bacterial envelope is called the. cytoplasm. The correct morphological term for a rod shaped ... A bacterial process of recombination that involves the uptake of naked DNA is called. transformation. ... A bacterial process of recombination that requires cell to cell contact is called. conjugation. ...
Streptococcus suis Fimbria-Like Protein SssP1 Contributes to Bacterial Virulence. Streptococcus suis is an important zoonotic ... The mechanisms by which S. suis constructs these fimbria-like structures on the cell surface and adheres to host cells were ... e01385-18) demonstrated that SssP1, a novel fimbria-like protein transported by the SecY2/A2 system, contributes to the ...
... les souches nécrotoxinogènes de type NTEC2 produisent des adhésines de la famille des fimbriae F17. Ces fimbriae sont ... Ces résultats confirment le lien entre fimbriae F17 et NTEC2 et mettent en évidence le rôle évolutif de supports plasmidiques ... The F17-G3 encoding gene was exclusively carried by bacterial chromosome, notably on a pathogenicity island. The F17e-A ... les souches nécrotoxinogènes de type NTEC2 produisent des adhésines de la famille des fimbriae F17. Ces fimbriae sont ...
Conserved PAMPS e.g. gram -ve LPS or bacterial flagellin recognised by host epithelial cells and innate immune cells - ...
What are some extracellular bacterial structures? Flagella. endoflagella/axial filament. Pili/fimbriae. Capsule. Biofilm ... Through *sex pili that allow one bacterial cell adhere to another and exchange genetic material. ...
Do streptococcal fimbriae play a role in bacterial tonsillitis?. Annals of the Royal College of Surgeons of England 2005, 87(6 ... Whos talking to whom? Epithelial-bacterial pathogen interactions. In: International Symposium on Epithelial-Bacterial Pathogen ... Fontes CMGA, Ali S, Gilbert HJ, Hazlewood GP, Hirst BH, Hall J. Bacterial xylanase expression in mammalian cells and transgenic ... Co-integration and expression of bacterial and genomic transgenes in the pancreatic and intestinal tissues of transgenic mice. ...
Fimbriae Proteins / genetics. Fimbriae, Bacterial / genetics. Food Microbiology*. Polymerase Chain Reaction*. Salmonella / ... Antigens, Bacterial / genetics. DNA, Bacterial / analysis. Electrophoresis, Agar Gel. Escherichia coli / genetics, isolation & ... 0/Adhesins, Escherichia coli; 0/Antigens, Bacterial; 0/DNA, Bacterial; 0/SEF21 protein, Salmonella enteritidis; 147680-16-8/ ...
... upregulated type-1 fimbriae expression, and reduced bacterial motility. Type-1 fimbriae, flagella, SPI-1, and SPI-2 gene ... upregulated type-1 fimbriae expression, and reduced bacterial motility. Type-1 fimbriae, flagella, SPI-1, and SPI-2 gene ... Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. Postinfection ... Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. Postinfection ...
Yersinia pestis, Salmonella, Escherichia coli, fimbriae, adhesion, plague. Hao Shen. Listeria, Ebola, T lymphocyte, bacterial ...
Fimbriae. : Protruding the outer membrane of the cell wall, thin proteinaceous surface appendages aid and mediate bacterial ... Fimbriae: Protruding the outer membrane of the cell wall, thin proteinaceous surface appendages aid and mediate bacterial ... The development of novel approaches to combat bacterial infections has increased due to the ongoing emergence and documentation ... Goulbourne, P. A. and Ellen, R. P. (1991) Evidence that Porphyromonas (Bacteroides) gingivalis fimbriae function in adhesion to ...
... with major virulence factors being STII and STI toxins and F4 fimbriae). Curiously, two of the E. coli isolates consisted of ... The objective of the present work was to investigate the presence of viral (RVA, TGEV and PEDV) and bacterial (different E. ... Both viral and/or bacterial pathogens were found in 96% of the submissions, which represents a fairly high number of cases with ... Viral and bacterial investigations on the aetiology of recurrent pig neonatal diarrhoea cases in Spain. ...
Fimbriae: Protruding the outer membrane of the cell wall, thin proteinaceous surface appendages aid and mediate bacterial ... Goulbourne, P. A. and Ellen, R. P. (1991) Evidence that Porphyromonas (Bacteroides) gingivalis fimbriae function in adhesion to ... Two distinct fimbriae types are displayed on the cell surfaces of the bacteria, known as FimA and Mfa protein [8]. These ... Upon contact the bacterium must resist the plethora of host responses working against bacterial colonisation [13]. Host factors ...
Pili, optical tweezers, bacterial adhesion, fimbriae, uncoiling National Category Other Physics Topics Biophysics Identifiers. ... fimbriae, pili, uncoiling, damping, bacterial adhesion National Category Other Physics Topics Biophysics Research subject ... These pili, which are vital for bacterial adhesion, thereby serve as a new possible approach in the fight against bacterial ... The biophysical properties of CS2 fimbriae assessed in this work classify them into a low-force unwinding group of fimbriae ...
The FIMBUG project has provided new knowledge on the role of fimbriae in bacterial adhesion and biofilm formation. Also, new ... Comparison of the bacterial community composition in the granular and the suspended phase of sequencing batch reactors E. Szabo ... Comparison of the bacterial community composition in the granular and the suspended phase of sequencing batch reactors E. Szabo ... Fimbriae are hair-like surface organelles found on many pathogenic bacteria. These structures are known to strongly promote ...
Bacterial Cell Wall. Kevin D Young. Published online: April 2010. Bacterial Pili and Fimbriae. I‐Hsiu Huang, Prabhat Dwivedi, ... Bacterial Flagella. Kelly T Hughes, Marc Erhardt. Published online: October 2011. Peptidoglycan. Kevin D Young. Published ... Bacterial and Archaeal Inclusions. Jessup M Shively, Gordon C Cannon, Sabine Heinhorst, Donald A Bryant, Shiladitya DasSarma, ... Bacterial Membrane Transport: Organization of Membrane Activities. Etana Padan. Published online: September 2009. ...
2007) Acellular pertussis vaccines and the role of pertactin and fimbriae. Expert Rev Vaccines 6(1):47-56. ... Autotransporter-mediated bacterial clumping. Begoña Heras, Makrina Totsika, Kate M. Peters, Jason J. Paxman, Christine L. Gee, ... Autotransporter-mediated bacterial clumping. Begoña Heras, Makrina Totsika, Kate M. Peters, Jason J. Paxman, Christine L. Gee, ... Bacterial Cell-Aggregation Assays.. Plasmids encoding ag43a wild-type and mutant variants were transformed into the previously ...
  • P-fimbriae, bacterial adhesion, and pyelonephritis. (bmj.com)
  • In this study, we employed atomic force microscopy (AFM) to interrogate how the adhesion work and the viscoelasticity of a bacterial pathogen, Klebsiella pneumoniae, influence biofilm formation. (edu.au)
  • This study indicates that AFM-based adhesion measurements of bacteria can be used to evaluate the function of bacterial surface polymers in biofilm formation and to predict the ability of bacterial biofilm formation. (edu.au)
  • An E. coli bacterial infection starts with adhesion to a host cell using cell surface expressed adhesion polymers, called adhesion pili. (diva-portal.org)
  • These pili, which are vital for bacterial adhesion, thereby serve as a new possible approach in the fight against bacterial infections by targeting and disabling these structures using novel chemicals. (diva-portal.org)
  • The FIMBUG project has provided new knowledge on the role of fimbriae in bacterial adhesion and biofilm formation. (gu.se)
  • These include bacterial cell aggregation, adhesion to surfaces of host cells such as, in the case of gut bacteria, the linings of the intestine, adhesion to other microbial cells in biofilms , gene and protein injection into other cells, DNA uptake by naturally transformable bacteria, and virulence attributes of pathogenic bacteria. (citizendium.org)
  • Adhesion of bacterial cells has an important survival role in their survival as micro-colonies - called biofilms - on solid surfaces in the natural environment, and F-pili determine the final shapes of the structures seen in mature surface biofilms formed by Eschericha coli bacteria, as mutants affected in the plasmid specified F-pili form a biofilm of a different structure [7] . (citizendium.org)
  • In contrast, the agfBA mutant unable to form thin aggregative fimbriae still maintained long-range intercellular adhesion. (nih.gov)
  • AdrA mutant cells, which still formed thin aggregative fimbriae with all binding characteristics, exhibited community behaviour but, unlike the wild type, lacked long-range intercellular adhesion. (nih.gov)
  • PACs in cranberries are said to inhibit the adhesion capabilities of the fimbriae, causing the inhibited bacteria to pass through the urethra. (todaysdietitian.com)
  • P-fimbriae is a strain, or adhesion protein, in E. coli , which has bacterial tips that bind to the uroepithelium. (todaysdietitian.com)
  • Constructed by distinct assembly pathways resulting in diverse morphologies, fimbriae have been described to mediate functions including adhesion, motility, and DNA transfer. (asmscience.org)
  • Type 1 fimbriae are adhesion organelles expressed by many Gram-negative bacteria. (pnas.org)
  • Most members of Enterobacteriaceae have peritrichous Type I fimbriae involved in the adhesion of the bacterial cells to their hosts. (princeton.edu)
  • Genomic comparison between strains showed differences in regions containing a fimbriae/adhesion virulence protein. (ajtmh.org)
  • These fimbriae recognize receptors of the host cell surface and may improve bacterial adhesion. (scielo.br)
  • Here we highlight recent work demonstrating that surface attachment drives a transcriptional response in bacterial pathogens, including uropathogenic Escherichia coli (E. coli), and discuss the complexity of experimental design when dissecting the specific role of adhesion-mediated signaling during infection. (rutgers.edu)
  • Methods of inhibiting bacterial adhesion to medical implants and reducing device-associated infection are effectuated by administering an effective amount of apo-transferrin to an individual with such an implant. (google.com)
  • 1. A method of inhibiting adhesion of bacteria to an implanted device in an individual comprising administering to said individual an amount of apo-transferrin effective for inhibiting bacterial adhesion to the device. (google.com)
  • This invention relates to a method of inhibiting bacterial adhesion to biomaterials. (google.com)
  • More particularly, the invention relates to a method of reducing device-associated infections by inhibiting bacterial adhesion to biomaterials with apo-transferrin. (google.com)
  • These complications may be directly attributed to the presence of biomaterial, which is foreign to the human body and provides a suitable substrate for bacterial adhesion at a site sequestered from the body's immunological defenses. (google.com)
  • A Western blot analysis of the purified fimbrial preparation using an antiserum raised against native fimbriae suggested that fimbrial proteins did not carry any major sequential epitope and that, in native fimbriae, conformational epitopes, possibly generated between different subunits, might provide for the major immunogenic epitopes. (nih.gov)
  • FimB is one of the 2 regulatory proteins which control the phase variation of type 1 fimbriae in E.coli. (uniprot.org)
  • Despite their abundance and role in bacterial pathogenesis, most AT proteins have not been structurally characterized, and there is a paucity of detailed information with regard to their mode of action. (pnas.org)
  • Thus, the bacterial proteins able to interact with fimS are likely to be involved in regulating the expression of type 1 fimbria. (ncku.edu.tw)
  • These include the locus of enterocyte effacement (LEE)-encoded type III secretion system (T3SS), various T3-secreted effector proteins, H7 flagellin, and a number of specific adhesins, including F9 fimbriae and autotransporters. (frontiersin.org)
  • Bacterial fimbriae can contain lectin proteins generally at their tips. (citizendium.org)
  • AdrA is a putative transmembrane protein with a C-terminal GGDEF domain of unknown function although it is present in over 50 bacterial proteins. (nih.gov)
  • the chimeric proteins were exposed on the bacterial surface and the cholera toxin epitope was authentically displayed, i.e. it was recognized on bacteria by specific antiserum. (nih.gov)
  • The fimbriae is assembled by a chaperone-usher system, and proteins required for the assembly are expressed by the Pap operon, which is located on pathogenicity islands. (wikipedia.org)
  • Whereas toxins are generally released by the pathogens into the extracellular milieu and can thus act at distant sites, adhesins typically remain associated with the bacterial surface, allowing the microorganisms to adhere to host determinants such as glycolipids, proteoglycans, surface, and matrix proteins ( 1 - 5 ). (rupress.org)
  • Atomic resolution detail of the proteins in the fibers and analysis of genetic variability among different clinical strains were combined to show that each bacterial strain presents a different outer surface of the major protein while preserving the protein components that are buried within the fiber," said senior author Esther Bullitt, an associate professor in the department of physiology and biophysics at BUSM. (medicalnewstoday.com)
  • The interaction of AIEC with IEC depends on bacterial factors mainly type 1 pili, flagella, and outer membrane proteins. (plos.org)
  • The long-term goal of this laboratory is to understand how bacterial pathogens initiate their infectious process. (upenn.edu)
  • A better understanding of the structure and function of the microbial ligands and host receptors will help to design new prophylactic and therapeutic approaches against bacterial pathogens. (upenn.edu)
  • To circumvent the problem of drug-resistant bacterial pathogens, we need to develop new effective methods, substances, and materials that can disarm and prevent them from causing infections. (diva-portal.org)
  • My lab is focused on understanding the survival and virulence strategies employed by a group of important bacterial pathogens collectively known as E xtraintestinal P athogenic E scherichia c oli , or ExPEC. (utah.edu)
  • In this book, the authors present current research in the study of the virulence mechnisms, diagnosis and management of bacterial pathogens. (novapublishers.com)
  • It has recently become feasible to quantify all mRNAs encoded by the genomes of bacterial pathogens and their eukaryotic host cells and to apply this approach to study the interaction of Mycobacterium tuberculosis with its primary host cell, the macrophage. (stanford.edu)
  • Bacterial attachment to plants has predominantly been studied for its role in virulence of plant pathogens. (asm.org)
  • However, only one study has begun to address the fundamental questions of what allows animal bacterial pathogens to associate with plants initially and how they remain attached ( 14 ). (asm.org)
  • Nejsum, Lene N. 2017-08-01 00:00:00 To target bacterial pathogens that invade and proliferate inside host cells, it is necessary to design intervention strategies directed against bacterial attachment, cellular invasion and intracellular proliferation. (deepdyve.com)
  • The main research interests of the Koomey Group lie in studies of the molecular and cell biology of microbial pathogenesis, that is the study of how bacterial pathogens cause disease in man. (pewtrusts.org)
  • Together, Tfp expressing bacterial pathogens are responsible for an extensive amount of morbidity and mortality worldwide. (pewtrusts.org)
  • The expression of virulence by bacterial pathogens often requires the production and action of toxins and adhesins. (rupress.org)
  • Whether bats carry clinically significant bacterial pathogens is unknown. (thefreelibrary.com)
  • An inflammatory pathology is a disease condition where the host's immune defences constantly encounter invading pathogens or bacterial virulence factors. (hindawi.com)
  • Bacterial adhesins and heat-labile (LT) and heat-stable (ST) enterotoxins are the virulence determinants in ETEC diarrhea. (asm.org)
  • The virulence determinants of ETEC in diarrhea are bacterial adhesins and enterotoxins ( 1 , 7 , 24 , 25 , 37 , 41 , 43 ). (asm.org)
  • Adhesins mediate the attachment of ETEC bacteria to host epithelium cells in the small intestine and facilitate subsequent bacterial colonization. (asm.org)
  • The AC/I-fimbriae (Fac) which are expressed by avian-pathogenic E. coli strains also belong to the S-family of adhesins ( 5 , 6 ). (asm.org)
  • The uropathogenic E. coli strain 536 (O6:K15:H31) has previously been shown to produce various types of fimbrial adhesins, including type 1, P-related, and S-fimbriae ( 26 ). (asm.org)
  • Among pathogenic E. coli in calves, necrotoxigenic strains of NTEC2 type produce adhesins of the F17 fimbriae family. (archives-ouvertes.fr)
  • Fimbriae are adhesins that attach to specific sugar based receptors on uroepithelial cells. (sciencebasedmedicine.org)
  • The sweet connection: Solving the riddle of multiple sugar-binding fimbrial adhesins in Escherichia coli: Multiple E. coli fimbriae form a versatile arsenal of sugar-binding lectins potentially involved in surface-colonisation and tissue tropism. (semanticscholar.org)
  • Among them, chaperone-usher (CU) fimbriae adhesins, related to prototypical type 1 fimbriae, interact in highly specific ways with different ligands at different stages of bacterial infection or surface colonisation. (semanticscholar.org)
  • Uropathogenic E. coli express a variety of adhesins, including P and type I fimbriae. (urologyweb.com)
  • In summary, infections are possible when several conditions coexist, such as the finding of specific receptor sites on the urinary tract cells such as glycolipids and mannose, for certain uropathogens such as E. coli and enterococcus faecalis, and factors such as lipopolysaccharides (0 antigens), capsular antigens (K), adhesins (particularly type P and I fimbriae) and hemolysin. (urologyweb.com)
  • UPEC expresses multiple adhesins and virulence factors that provoke inflammation and enable bacterial colonization of the bladder as the first step in UTI pathogenesis ( 2 - 5 ). (jimmunol.org)
  • More recently, it has become apparent that some bacterial adhesins are, in part, released from the cell surface into the extracellular milieu ( 6 - 11 ). (rupress.org)
  • These interactions of fimbriae and nonfimbrial adhesins with their respective receptors provide a dynamic surface matrix for subsequent colonization of Haemophilus parainfluenzae ( Hpar ), Propionibacterium acnes ( Pacn ), Prevotella loescheii ( Ploe ), Viellonella spp. (asmscience.org)
  • Linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus Borrelia notably Borrelia burgdorferi , which causes Lyme disease. (wikipedia.org)
  • Chaperone-usher (CU) fimbriae are adhesive surface organelles common to many Gram-negative bacteria. (nih.gov)
  • Bacteria use adherence fimbriae (pili) to overcome the body's defense mechanism and cause disease. (microbeonline.com)
  • Fimbriae are hair-like surface organelles found on many pathogenic bacteria. (gu.se)
  • The bacterial capsule is a large structure common to many bacteria. (wikipedia.org)
  • The capsule-which can be found in both gram negative and gram-positive bacteria-is different from the second lipid membrane - bacterial outer membrane, which contains lipopolysaccharides and lipoproteins and is found only in gram-negative bacteria. (wikipedia.org)
  • In some cases, mutant pathogenic bacteria that lack fimbriae cannot adhere to their usual target host cell surfaces, and thus cannot cause disease . (citizendium.org)
  • Many bacteria contain other extracellular structures such as flagella and fimbriae which are used for motility (movement), attachment, and conjugation respectively. (citizendium.org)
  • Some bacteria also contain capsules or slime layers that also facilitate bacterial attachment to surfaces and biofilm formation. (citizendium.org)
  • P fimbriae (also known as pyelonephritis-associated pili) or P pili or Pap are chaperon-usher type (specifically of the π family) fimbrial appendages found on the surface of many Escherichia coli bacteria. (wikipedia.org)
  • For example, bacteria adhere to cells with hairlike fimbriae (pili). (todaysdietitian.com)
  • Shorter pili called fimbriae help bacteria attach to surfaces. (majortests.com)
  • The fimbriae on the surface of the E. coli enable the bacteria to bind to specific carbohydrate receptors on the surface of uroepithelial cells. (urologyweb.com)
  • P fimbriae are commonly found on bacteria, causing pyelonephritis but not cystitis or asymptomatic bacteria. (urologyweb.com)
  • Researchers at Boston University School of Medicine (BUSM), the Naval Medical Research Center and the National Institutes of Health, have solved the structure of thin hair-like fibers called "pili" or "fimbriae" on the surface of bacteria that cause traveler's diarrhea . (medicalnewstoday.com)
  • Most bacteria do not grow on laboratory media, and next-generation sequencing technologies have proven useful for studying bacterial species diversity and dynamics, even in complex systems like the gut (8). (thefreelibrary.com)
  • Fimbriae mediate the attachment of many pathogenic bacteria to host cells. (biomedcentral.com)
  • The bacteria use surface pili or fimbriae to attach or bind to t. (bio-medicine.org)
  • The fluid that surrounds the central nervous system, provides nutrient/waste product transport to the brain, and acts as the medium for bacteria growth in bacterial meningitis. (jems.com)
  • bacterial pathogenesis are key to design effective treatments. (kenyon.edu)
  • and the role of HtrA as a chaperone and protease in bacterial pathogenesis. (novapublishers.com)
  • P fimbriae mediate adherence to host cells, a key event in the pathogenesis of urinary tract infections. (wikipedia.org)
  • These results show that type 1 fimbriae in the genetic background of a uropathogenic strain contribute to the pathogenesis of E. coli in the urinary tract. (pnas.org)
  • Excepting the toxemic syndromes determined by toxic-shock syndrome toxin 1 (TSST-1), scalded skin syndrome as a result of epidermolytic toxins activity, and food poisoning as a consequence of ingestion of preformed enterotoxins, the pathogenesis of S. aureus is the result of interaction between a variety of host factors and the bacterial virulence determinants [ 1 ]. (mdpi.com)
  • To address the role of adhesin release in pathogenesis, we used Bordetella pertussis as a model, since its major adhesin, filamentous hemagglutinin (FHA), partitions between the bacterial surface and the extracellular milieu. (rupress.org)
  • ETEC uses surface "pili" or "fimbriae" to attach to host intestinal epithelia, an early, vital step in diarrhea pathogenesis. (medicalnewstoday.com)
  • In the pathogenesis of PD, subgingival complex bacterial biofilm induces inflammation that leads to connective tissue degradation and alveolar bone resorption around the teeth. (hindawi.com)
  • These fimbriae are essential for the attachment of the pathogen to host epithelial tissues and mediate a form of surface translocation known as twitching motility, which is implicated in the spread of infection ( 23 ) or the aggregation into microcolonies during biofilm formation ( 30 ). (asm.org)
  • The mechanism by which fimbriae promote biofilm formation and spreading of antibiotic resistance genes are not well understood. (gu.se)
  • The phenotype of yqi C mutant exhibited few and short flagella, fimbriae on the cell surface, enhanced biofilm formation, upregulated type-1 fimbriae expression, and reduced bacterial motility. (frontiersin.org)
  • Aggregation and biofilm formation are critical mechanisms for bacterial resistance to host immune factors and antibiotics. (pnas.org)
  • These results suggested that the FimA fimbriae promote initial biofilm formation but exert a restraining regulation on biofilm maturation, whereas Mfa and Kgp have suppressive and regulatory roles during biofilm development. (biomedcentral.com)
  • We first evidenced that the cupE gene cluster was specifically expressed in biofilm conditions and was responsible for fibre assembly containing at least CupE1 protein, at the bacterial cell surface. (dtu.dk)
  • These fimbriae not only played a significant role in the early stages (microcolony and macrocolony formation) but also in shaping 3D mushrooms during P. aeruginosa biofilm development. (dtu.dk)
  • The transformed strain expressing a plasmid containing genes encoding the WT fimbrial subunit and the prepilin peptidase displayed all of the hallmarks of a fimbriated bacterium including the distinct star-like colony morphology, robust biofilm formation, biofilm architecture composed of discrete microcolonies and the presence of fimbriae. (microbiologyresearch.org)
  • Colonization and establishment of infection by P. aeruginosa are dependent on the production of a number of virulence factors, including extracellular toxins, proteases, lipases, siderophores, and polar filamentous structures called type IV fimbriae. (asm.org)
  • Colonization factor antigen I (CFA/I) is the archetype of eight genetically related fimbriae of enterotoxigenic Escherichia coli (ETEC) designated class 5 fimbriae. (asm.org)
  • ETEC typically attaches to host cells via filamentous bacterial surface structures known as colonization factors (CFs). (asm.org)
  • Our research is directed towards bacterial ligands that bind to specific host receptors and mediate bacterial colonization, host cell signaling, and/or optimal toxin delivery. (upenn.edu)
  • However, the role of yqiC in bacterial colonization and host immunity remains unknown. (frontiersin.org)
  • Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. (frontiersin.org)
  • Our results suggest that the filamentous haemagglutinin plays a more crucial role than fimbriae in the colonization of the upper respiratory tract of the mouse. (nih.gov)
  • The FimH subunit of type 1-fimbriated Escherichia coli has been implicated as an important determinant of bacterial adherence and colonization of the urinary tract. (jci.org)
  • In humans, proteases can act as host defence mechanisms to counteract bacterial colonization. (plos.org)
  • It is believed that vaccines inducing anti-adhesin immunity to inhibit bacterial adherence and anti-toxin immunity to eliminate toxin activity would provide broad-spectrum protection against ETEC. (asm.org)
  • Subclass divisions generally correlate with distinguishing in vitro adherence phenotypes of strains bearing the ETEC fimbriae. (asm.org)
  • Bacterial adherence inhibition by serum and fecal samples from rabbits immunized with chimeric K88ac fimbriae. (asm.org)
  • Porcine ETEC strain 3030-2 that expresses K88ac fimbriae and porcine cell line IPEC-J2 expressing K88ac receptors were used in adherence inhibition assays. (asm.org)
  • We validated and extended these findings by demonstrating that antibodies directed at the putative binding region of FimH or at synthetic peptides corresponding to epitopes within the binding domain could specifically block type 1 fimbriae-mediated bacterial adherence to bladder epithelial cells in situ and yeast cells in vitro. (jci.org)
  • The mechanism of protection was attributed, at least in part, to inhibition of bacterial adherence to the bladder surface by s-FimH1-25-specific antibody molecules that had filtered through the kidneys into the urine. (jci.org)
  • Adherence to host uroepithelial cells is a crucial step during the infection that allows uropathogenic E.coli to colonize the urinary tract and prevents bacterial removal during micturition. (wikipedia.org)
  • S. Typhimurium is capable of producing up to 13 distinct surface structures called fimbriae that presumably mediate its adherence to surfaces. (asm.org)
  • it is used in bacterial cell conjugation, and (b) a smaller form which is of major significance in adherence of bacterial cells to epithelial surfaces such as the intestinal mucosa or mammary gland epithelium. (thefreedictionary.com)
  • The Actinomyces fimbrial system is a versatile adhesive principle for promoting bacterial coaggregation and host tissue adherence that leads to the development of one of the most complex biofilms, the dental plaque. (asmscience.org)
  • The response regulator AlgR is required for both alginate biosynthesis and type IV fimbria-mediated twitching motility in Pseudomonas aeruginosa . (asm.org)
  • Search Results for 'bacterial-type flagellum-dependent swarming motility' AND ARG_TAG:flag - 8 interactions found in 5 entries. (helmholtz-muenchen.de)
  • Flagella are found primarily in bacterial rods and are used for motility. (cliffsnotes.com)
  • Therefore, YqiC can negatively regulate FimZ for type-1 fimbriae expression and manipulate the functions of its downstream virulence factors including flagella, SPI-1, and SPI-2 effectors. (frontiersin.org)
  • 2. Identify and functionally define bacterial fitness and virulence factors, including small regulatory RNAs and other regulators, that enable ExPEC isolates to resist the multitude of environmental stresses encountered during the course of an infection. (utah.edu)
  • A) Antibodies against PT and FHA neutralize secreted virulence factors and mitigate disease progression but are not targeted to the bacterial surface. (cdc.gov)
  • Porphyromonas gingivalis , a periodontal pathogen, expresses a number of virulence factors, including long (FimA) and short (Mfa) fimbriae as well as gingipains comprised of arginine-specific (Rgp) and lysine-specific (Kgp) cysteine proteinases. (biomedcentral.com)
  • P. gingivalis expresses a broad range of virulence factors, of these cysteine proteases (gingipains) are of special importance both for the bacterial survival/proliferation and for the pathological outcome. (intechopen.com)
  • To do this, the wild-type, type 3 fimbriae-deficient, and type 3 fimbriae-overexpressed K. pneumoniae strains have been investigated in an aqueous environment. (edu.au)
  • Immunolabeling TEM images showing biosynthesized K88ac chimeric fimbriae of the 8551, 8549, 8576, and 8616 strains. (asm.org)
  • Display of chimeric fimbriae was tested with respect to host background in three different Escherichia coli strains, i.e. an isogenic set of K-12 strains, differing in the presence of an indigenous fim gene cluster, as well as a wild-type isolate. (nih.gov)
  • 1959. Fimbriae and adhesive properties in Klebsiella strains. (asmscience.org)
  • Among the characterized genes were strains with insertions in the intergenic region between agfB , the surface-exposed aggregative fimbria (curli) nucleator, and agfD , a transcriptional regulator of the LuxR superfamily, and rpoS , the stationary-phase sigma factor. (asm.org)
  • Bacterial strains, media, and culture conditions. (asm.org)
  • P-fimbriae were present in 91% (33/35) of the urinary strains causing acute pyelonephritis. (sciencebasedmedicine.org)
  • Among strains causing cystitis and asymptomatic bacteriuria P-fimbriae were found in 19% and 14% of cases, respectively. (sciencebasedmedicine.org)
  • Factor Afa (encoded by afa gene) is an afimbrial adhesin, and together with P and S fimbriae, have been epidemiologically related to E. coli strains that cause UTI in humans and pets (4). (scielo.br)
  • Three inter-related areas, intestinal nutrient transport, intestinal drug transport and epithelial-bacterial pathogen interactions are the focus of current research. (ncl.ac.uk)
  • We present an automated microscopy-based, fast, high-throughput method for analyzing size and number of intracellular bacterial colonies in infected tissue culture cells.Cells are seeded in 48-well plates and infected with a GFP-expressing bacterial pathogen. (deepdyve.com)
  • Bats as reservoir hosts of human bacterial pathogen, Bartonella mayotimonensis. (thefreelibrary.com)
  • sex pilus, - transfer from one bacterial cell to another- conjugation. (powershow.com)
  • F-pili are involved in bacterial conjugation and connect the bacterium to another bacterium and enable a bridge between the cytoplasms of the cells which becomes the channel for one way transfer of a single-strand of DNA and certain protein molecules. (citizendium.org)
  • Schematic drawing of bacterial conjugation. (citizendium.org)
  • Fimbriae (pili) are shorter, straighter and more numerous than bacterial flagella and are composed by subunits of protein called pilin. (microbeonline.com)
  • Type-1 fimbriae, flagella, SPI-1, and SPI-2 gene expression was quantified using real-time PCR. (frontiersin.org)
  • Respiratory enzymes are located at the cell membrane of prokaryotes, and the membrane assists DNA replication and has attachment points for bacterial flagella. (cliffsnotes.com)
  • The mechanisms by which S. suis constructs these fimbria-like structures on the cell surface and adheres to host cells were also elucidated. (asm.org)
  • What are some extracellular bacterial structures? (brainscape.com)
  • These structures are known to strongly promote formation of bacterial biofilms on tissue and biomaterials. (gu.se)
  • Type 1 fimbriae are filamentous structures on Escherichia coli. (ncku.edu.tw)
  • The helical structures of Bordetella pertussis fimbriae of serotypes 2 and 6 were determined by optical diffraction analysis of electron micrographs of negatively stained paracrystalline bundles of purified fimbriae. (asm.org)
  • tubulare, hairlike structures of protein larger and more rare than fimbriae. (powershow.com)
  • P fimbriae are large, linear structures projecting from the surface of the bacterial cell. (wikipedia.org)
  • Proteinaceous, nonflagellar surface appendages constitute a variety of structures, including those known variably as fimbriae or pili. (asmscience.org)
  • Pili - Hair-like structures on the surface of the cell that attach to other bacterial cells. (majortests.com)
  • Identifying fimbriae produced during infection will provide important insights into how these bacterial structures contribute to. (asm.org)
  • The terms pili and fimbriae are often used interchangeably. (citizendium.org)
  • Note-the above value uses pili and fimbriae interchangeably. (harvard.edu)
  • Construction and analysis of Bordetella pertussis mutants defective in the production of fimbriae. (nih.gov)
  • Although the role of fimbriae in bacterial disease has been well established, little is known about the function of Bordetella pertussis fimbriae. (nih.gov)
  • Helical structure of Bordetella pertussis fimbriae. (asm.org)
  • To investigate the significance of adhesin release during bacterial infection, we used Bordetella pertussis , the whooping cough agent, as a model. (rupress.org)
  • Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection. (biomedsearch.com)
  • This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)-cholera toxin B subunit (CTB) in the intranasal murine model of infection. (biomedsearch.com)
  • Type 1 fimbriae have been purified from a Salmonella typhi strain of clinical origin. (nih.gov)
  • The yqiC gene of Salmonella enterica serovar Typhimurium ( S . Typhimurium) regulates bacterial growth at different temperatures and mice survival after infection. (frontiersin.org)
  • Here we show that dopamine increases bacterial iron incorporation and promotes Salmonella Typhimurium growth both in vitro and in vivo . (asm.org)
  • Salmonella enterica is the leading cause of bacterial food-borne infection in the United States. (asm.org)
  • The 987P-like CS18 fimbriae of human enterotoxigenic Escherichia coli ON phase variants. (upenn.edu)
  • Constructed K88ac-toxin chimeric fimbriae were harvested and used for rabbit immunization. (asm.org)
  • Similarly, chimeric fimbriae with an STa peptide expressed in ETEC adhesin CS31A elicited neutralizing anti-STa antibodies ( 4 , 5 ). (asm.org)
  • All of the chimeric fimbriae were recognized by hybridoma supernatant of anti-K88ac MAbs 36/41 and 30/17, whereas chimeric fimbriae carrying the LTP1 and STa 13 epitopes were detected by anti-CT and anti-STa antisera. (asm.org)
  • Immunization of rabbits with purified chimeric fimbriae resulted in serum which specifically recognized cholera toxin B chain, confirming the utility of the employed strategy. (nih.gov)
  • To correlate this with functional attributes, we made antibodies against CFA/I and CS17 whole fimbriae and maltose-binding protein fusions with the amino-terminal half of the corresponding minor subunits. (asm.org)
  • The potential of the major structural protein of type 1 fimbriae as a display system for heterologous sequences was tested. (nih.gov)
  • P fimbriae can trigger release of interleukins and C reactive protein and bring about reduced renal concentrating capacity in patients with a UTI in addition to causing inflammation and scarring. (urologyweb.com)
  • These may either be true type 4 fimbriae, or components of protein complexes which act in the transport of macromolecules (DNA or protein) into or out of the cell. (ingentaconnect.com)
  • Pili (singular: pilus, or "hair") are a second type of protein structure projecting beyond the bacterial surface (Eisenstein, 1987). (harvard.edu)
  • Purified fimbriae retained their ability to bind to erythrocytes in a mannose-inhibitable fashion and, in doing so, behaved preferentially as a monovalent adhesin. (nih.gov)
  • Data from this study demonstrated that K88ac fimbriae expressing LT and STa epitope antigens elicited neutralizing anti-toxin antibodies and anti-adhesin antibodies and suggested that E. coli fimbriae could serve as a platform for the development of broad-spectrum vaccines against ETEC. (asm.org)
  • These fimbriae are composed of the polymorphic pilin F17A and adhesin F17G, for which several variants are unknown. (archives-ouvertes.fr)
  • Localization of a domain in the FimH adhesin of Escherichia coli type 1 fimbriae capable of receptor recognition and use of a domain-specific antibody to confer protection against experimental urinary tract infection. (jci.org)
  • The binding of the P fimbriae to epithelial cells is mediated by the tip adhesin PapG. (wikipedia.org)
  • This work provides insight into the structure-function mechanisms that facilitate bacterial interactions during infection. (pnas.org)
  • Because binding to the host cells is the first step of infection, type 1 fimbria is an important virulence factor of pathogenic E. coli. (ncku.edu.tw)
  • First recurrence of symptomatic urinary tract infection, defined as bacterial growth ≥10 5 colony forming units/ml in a clean voided midstream urine specimen. (bmj.com)
  • This study presents evidence that type 1 fimbriae increase the virulence of Escherichia coli for the urinary tract by promoting bacterial persistence and enhancing the inflammatory response to infection. (pnas.org)
  • No. It is the minority of E. coli that cause UTI that have fimbria, and the presence of fimbriae may be more important for the development of pyelonephritis (kidney infection) than cystitis (bladder infection) . (sciencebasedmedicine.org)
  • Multiple 48-well plates can be processed sequentially and the procedure can be completed in one working day.As a model we quantified intracellular bacterial colonies formed by uropathogenic Escherichia coli (UPEC) during infection of human kidney cells (HKC-8). (deepdyve.com)
  • This is due to the reduction of bacterial factors necessary for infection, which is likely to be caused by the retention of. (asm.org)
  • Early transcriptional activation events that occur in bladder immediately following bacterial urinary tract infection (UTI) are not well defined. (jimmunol.org)
  • These fibers, which are needed for ETEC infection to take place in the intestines, exit the bacterium through a pore on the bacterial surface, the researchers explained in a news release from the Boston University School of Medicine. (bio-medicine.org)
  • Our findings suggest that the minor subunits of class 5 fimbriae may be superior to whole fimbriae in inducing antiadhesive immunity. (asm.org)
  • Despite serological and biochemical differences between type 2 and type 6 fimbriae, the packing arrangements of their fimbrial subunits are identical. (asm.org)
  • From this observation, we infer that the respective subunits may have in common conserved regions whose packing dictates the helical geometry of the fimbria. (asm.org)
  • Construction of chimeric faeG genes and detection of chimeric FaeG major subunits of E. coli K88ac fimbriae. (asm.org)
  • This locus, widely conserved in different bacterial species, contains four additional genes encoding non-archetypal fimbrial subunits. (dtu.dk)
  • There was, however, a reduction in the secretion of fimbrial subunits, and fewer fimbriae were observed on the surface of the mutant strain. (microbiologyresearch.org)
  • The genes for the major subunit of two of the other related fimbriae have been reported previously ( 13 , 15 ). (asm.org)
  • The phase variation is regulated by the flipping of the 314-bp fimS fragment, which contains the promoter driving the expression of the genes required for the synthesis of type 1 fimbria. (ncku.edu.tw)
  • The Pseudomonas aeruginosa genes pilB‐D and pilQ are necessary for the assembly of type 4 fimbriae. (ingentaconnect.com)
  • Homologues of these genes and of the subunit (pilin) gene have been described in various different bacterial species, but not always in association with type 4 fimbrial biosynthesis and function. (ingentaconnect.com)
  • The S fimbria (encoded by sfa genes) has also been implicated in the process of UTI. (scielo.br)
  • Many persistent and chronic bacterial infections are associated with the formation of large cell aggregates and biofilms that are difficult to treat. (pnas.org)
  • In periodontal disease (PD) there is paucity of information concerning the molecular pathways in the host cells, which could regulate their inflammatory response to complex subgingival bacterial biofilms. (hindawi.com)
  • Microbial biofilms develop on the surfaces of medical devices and proceed to cause full blown bacterial infections and sepsis. (mdpi.com)
  • Estimates by Costerton attribute more than half of bacterial infections in immuno-compromised patients to slime encased microbial colonies (biofilms) [ 5 ]. (mdpi.com)
  • Some bacterial viruses ( bacteriophages ) attach to receptors on sex pili at the start of their reproductive cycle. (citizendium.org)
  • K88ac fimbriae purified from ETEC wild-type strain 3030-2 were used as antigens to titrate anti-K88ac antibodies in an ELISA. (asm.org)
  • Antigens of the type-1 fimbriae of salmonellae and other enterobacteria. (asmscience.org)
  • Most bacterial capsules are composed of polysaccharide, but some species use other materials, such as poly-D-glutamic acid in Bacillus anthracis. (wikipedia.org)
  • The activation of immune cells leads to a release of an array of inflammatory mediators, for example, cytokines, chemokines, proteases, reactive oxygen species (ROS), and eicosanoids, which struggle against the bacterial burden. (intechopen.com)
  • Some bacterial species also have magnetosomes , which contain magnetic substances to help orient the organisms to hospitable environments. (cliffsnotes.com)
  • The mannose-specific fimbriae of type 1 are expressed in many enterobacterial species. (biomedcentral.com)
  • An epitope from the B subunit of LT toxin (LTP1, 8 LCSEYRNTQIYTIN 21 ) and an STa toxoid epitope ( 5 CCELCCNPQCAGCY 18 ) were embedded in the FaeG major subunit of E. coli K88ac fimbriae. (asm.org)
  • The P fimbriae is considered to be one of the most important virulence factor in uropathogenic E. coli and plays an important role in upper urinary tract infections. (wikipedia.org)
  • The prevalence of uropathogenic Escherichia coli bearing type 1 and/or p fimbriae was assessed in 179 adult women with urinary tract infections, and the presence of specific fimbriae types was correlated with results of localization studies. (sciencebasedmedicine.org)
  • Urinary tract infections caused by UPEC are among the most common bacterial infectious diseases in humans. (deepdyve.com)
  • They represent opportunistic infections arising from the patient's own bacterial personal flora. (urologyweb.com)
  • The dominant antibiotics used for treating infections today are the β -lactam antibiotics, which inhibit transpeptidases participating in bacterial cell wall synthesis. (hindawi.com)
  • The vaccination as a primary defence strategy and the development of new vaccines of broad specificity offer an important tool to use against bacterial infections. (biomedcentral.com)
  • Persistent bacterial infections associated with implant devices remain a serious and costly complication with both temporary and permanent implants. (google.com)
  • Hopefully, your detailed history and exam have lead you to the correct diagnosis, because I'm talking about infections of the central nervous system (CNS)--specifically bacterial meningitis . (jems.com)
  • The level of FimH antibodies entering the bladder from the circulatory system of the immunized mice was found to be markedly enhanced upon bacterial challenge. (jci.org)
  • B) Antibodies attaching to fimbriae poorly activate the complement system far from the bacterial membrane. (cdc.gov)
  • To do this, they make fimbriae, proteinaceous fibers on the bacterial cell wall. (sciencebasedmedicine.org)
  • According to the researchers, this study elucidates the mechanics of assembly of the fimbriae helical fibers as they exit the bacterium through a pore on the bacterial surface. (medicalnewstoday.com)
  • Capsular polysaccharides and fimbriae are two major components on a bacterial surface, which are critical for mediating cell-surface interactions. (edu.au)
  • Found mainly in Gram negative organisms, Fimbriae or pili ( singlular: pilus ) are hair like filaments (tiny hollow projections) that extend from the cell membrane into the external environment. (microbeonline.com)
  • What component of the bacterial cell wall gives it rigidity? (studystack.com)
  • Through *sex pili that allow one bacterial cell adhere to another and exchange genetic material. (brainscape.com)
  • It is a polysaccharide layer that lies outside the cell envelope, and is thus deemed part of the outer envelope of a bacterial cell. (wikipedia.org)
  • When viewed, bacterial capsules appear as a bright halo around the cell on a dark background. (wikipedia.org)
  • Bacterial cell structure Quellung reaction, a method to visualize capsule under a microscope Peterson JW (1996). (wikipedia.org)
  • Ultrastructure of bacterial cell. (powershow.com)
  • Escherichia coli cell with bacterial fimbriae (pili). (citizendium.org)
  • They generally have cell walls , like plant and fungal cells , but bacterial cell walls are normally made out of peptidoglycan instead of cellulose (as in plants ) or chitin (as in fungi and arthropods), and are not homologous with eukaryotic cell walls. (citizendium.org)
  • The most important bacterial structural characteristic is the cell wall . (citizendium.org)
  • This is followed by automated microscopy and subsequent semi-automated spot detection to determine the number of intracellular bacterial colonies, their size distribution, and the average number per host cell. (deepdyve.com)
  • Capsule - Found in some bacterial cells, this additional outer covering protects the cell when it is engulfed by other organisms, assists in retaining moisture, and helps the cell adhere to surfaces and nutrients. (majortests.com)
  • Cell Wall - Outer covering of most cells that protects the bacterial cell and gives it shape. (majortests.com)
  • This chapter provides an account of the current knowledge of a key adhesive principle, the fimbriae of A. oris , which are considered to be the main players for the cell-cell and cell-substratum interactions involving the early colonizers of dental plaque. (asmscience.org)
  • Bacterial diseases such as chronic periodontal disease evoke the host's immune responses, involving both the innate and adaptive signalling mechanisms with cell recruitment from the systemic circulation (macrophages, plasma cells, and T and B lymphocytes) that infiltrate the gingival soft tissues [ 1 ]. (hindawi.com)
  • Physiology of the Bacterial Cell. (harvard.edu)
  • Deletion of spr in the neonatal meningitis E. coli strain RS218 significantly increased the ratio of the bacterial colonies that contained the type 1 fimbria phase-ON cells on agar plates. (ncku.edu.tw)
  • Intracellular colonies are known to be clonal, originating from single invading UPEC.In our experimental setup, we found UPEC CFT073 intracellular bacterial colonies to be heterogeneous in size and present in nearly one third of the HKC-8 cells.This high-throughput experimental format substantially reduces experimental time and enables fast screening of the intracellular bacterial load and cellular distribution of multiple bacterial isolates. (deepdyve.com)
  • P fimbria is a hemagglutinin which recognizes a P blood group antigen of glycolipids found on uroepithelial cells. (urologyweb.com)
  • A bacterial artificial chromosome (BAC) library was generated from a derivative of the sequenced E. coli O157:H7 Sakai strain. (frontiersin.org)
  • E. coli CN1016 reconstituted with type 1 fimbriae (E. coli CN1018) had restored virulence similar to that of the wild-type parent strain. (pnas.org)
  • However, when compared with the B. pseudomallei -1026b strain, the Gabonese isolate was significantly less virulent in terms of bacterial dissemination, inflammatory response, and organ damage in mice. (ajtmh.org)
  • To investigate further the relationship between MorC and fimbriation, we identified and complemented the defect in fimbriae production in the afimbriated laboratory strain. (microbiologyresearch.org)
  • Also, new methods for investigating bacterial surface interactions, especially in realistic flow conditions, have been developed. (gu.se)
  • This complex activates complement to form a MAC, activates complement to deposit components such as C3b that opsonize the bacterial surface, and binds FcRs on phagocytes to activate phagocytosis. (cdc.gov)
  • Escherichia coli K-12 possesses multiple cryptic but functional chaperone-usher fimbriae with distinct surface specificities. (semanticscholar.org)
  • Our research is focused on studies of bacterial surface organelles termed Type IV pili (Tfp) or fimbriae. (pewtrusts.org)
  • CFA/I is the archetype of a family of eight ETEC fimbriae that share genetic and biochemical features ( 9 , 14 , 15 , 24 ). (asm.org)
  • Nucleoid Region - Area of the cytoplasm that contains the single bacterial DNA molecule. (majortests.com)
  • Evaluation of the distribution and prevalence of CU fimbrial types among different pathogenic and phylogenic groups provides an overview of group specific fimbrial profiles and insight into the ancestry and evolution of CU fimbriae in E. coli. (nih.gov)
  • I propose that bacterial pathogenicity is the result of multiple events in any given bacterium (vs. singular events) that occurred after the Fall and that no intentional pathogenic mechanisms exist. (answersingenesis.org)
  • Our study concentrates on a glycoconjugate vaccine based on covalently attached bacterial antigen to Klebsiella fimbriae type 1 and 3. (biomedcentral.com)
  • The type 3 of Klebsiella fimbriae are moderate inductors of IL-6 and interferon, whereas type 1 is even less potent inductor. (biomedcentral.com)
  • The potential broad spectrum activity of the protective FimH antibody was indicated from its serologic cross-reactivity with various urinary tract bacterial isolates bearing type 1 fimbriae. (jci.org)
  • In a clinical study, we observed that disease severity was greater in children infected with E. coli O1:K1:H7 isolates expressing type 1 fimbriae than in those infected with type 1 negative isolates of the same serotype. (pnas.org)
  • The E. coli O1:K1:H7 isolates had the same electrophoretic type, were hemolysin-negative, expressed P fimbriae, and carried the fim DNA sequences. (pnas.org)
  • AgfD has already been identified to regulate the extracellular matrix associated with the multicellular morphotype composed of thin aggregative fimbriae (agf). (nih.gov)
  • Bacteroides (Porphyromonas) gingivalis fimbriae activate mouse peritoneal macrophages and induce gene expression and production of interleukin-1. (nii.ac.jp)
  • The bacterial plaque formed by P. gingivalis results in inflammation of the periodontal tissues, resulting in epithelial migration and the destruction of the alveolar bone and periodontal ligament (12). (kenyon.edu)
  • These observations suggest the potential hazards of pharmacological catecholamine administration in patients with bacterial sepsis but also suggest that the inhibition of bacterial iron acquisition might provide a useful approach to antimicrobial therapy. (asm.org)
  • Environmental factors regulating adrA correspond to the regulation of thin aggregative fimbriae. (nih.gov)
  • Allelic variation contributes to bacterial host specificity. (upenn.edu)
  • We are working to delineate both bacterial and host factors that control the ability of ExPEC to colonize and persist within diverse host environments, with a major goal being the development of improved anti-bacterial therapeutics. (utah.edu)
  • Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae: effects of insert position and host background. (nih.gov)