Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Specific molecular sites or structures on cell membranes that react with FIBROBLAST GROWTH FACTORS (both the basic and acidic forms), their analogs, or their antagonists to elicit or to inhibit the specific response of the cell to these factors. These receptors frequently possess tyrosine kinase activity.
A 17-kDa single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. It binds to HEPARIN, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 2).
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Established cell cultures that have the potential to propagate indefinitely.
A fibroblast growth factor receptor with specificity for FIBROBLAST GROWTH FACTORS; HEPARAN SULFATE PROTEOGLYCAN; and NEURONAL CELL ADHESION MOLECULES. Several variants of the receptor exist due to multiple ALTERNATIVE SPLICING of its mRNA. Fibroblast growth factor receptor 1 is a tyrosine kinase that transmits signals through the MAP KINASE SIGNALING SYSTEM.
A fibroblast growth factor receptor that is found in two isoforms. One receptor isoform is found in the MESENCHYME and is activated by FIBROBLAST GROWTH FACTOR 2. A second isoform of fibroblast growth factor receptor 2 is found mainly in EPITHELIAL CELLS and is activated by FIBROBLAST GROWTH FACTOR 7 and FIBROBLAST GROWTH FACTOR 10. Mutation of the gene for fibroblast growth factor receptor 2 can result in craniosynostotic syndromes (e.g., APERT SYNDROME; and CROUZON SYNDROME).
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A fibroblast growth factor receptor that regulates CHONDROCYTE growth and CELL DIFFERENTIATION. Mutations in the gene for fibroblast growth factor receptor 3 have been associated with ACHONDROPLASIA; THANATOPHORIC DYSPLASIA and NEOPLASTIC CELL TRANSFORMATION.
A layer of vascularized connective tissue underneath the EPIDERMIS. The surface of the dermis contains innervated papillae. Embedded in or beneath the dermis are SWEAT GLANDS; HAIR FOLLICLES; and SEBACEOUS GLANDS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A sharply elevated, irregularly shaped, progressively enlarging scar resulting from formation of excessive amounts of collagen in the dermis during connective tissue repair. It is differentiated from a hypertrophic scar (CICATRIX, HYPERTROPHIC) in that the former does not spread to surrounding tissues.
A fibroblast growth factor that is a mitogen for KERATINOCYTES. It activates FIBROBLAST GROWTH FACTOR RECEPTOR 2B and is involved in LUNG and limb development.
Restoration of integrity to traumatized tissue.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
A fibroblast growth factor that is a specific mitogen for EPITHELIAL CELLS. It binds a complex of HEPARAN SULFATE and FIBROBLAST GROWTH FACTOR RECEPTOR 2B.
The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
Signal molecules that are involved in the control of cell growth and differentiation.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
A fibroblast growth factor receptor that is mainly expressed in LUNG; KIDNEY; PANCREAS; and SPLEEN. It also plays an important role in SKELETAL MUSCLE development and can contribute to NEOPLASTIC CELL TRANSFORMATION.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Any pathological condition where fibrous connective tissue invades any organ, usually as a consequence of inflammation or other injury.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.
A fibroblast growth factor that may play a role in regulation of HAIR FOLLICLE phenotype. Spontaneous mutation of the gene for this protein results in a strain of MICE with abnormally long hair, referred to as angora mice.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The inner membrane of a joint capsule surrounding a freely movable joint. It is loosely attached to the external fibrous capsule and secretes SYNOVIAL FLUID.
A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA.
The rate dynamics in chemical or physical systems.
A HEPARIN binding fibroblast growth factor that may play a role in LIMB BUDS development.
Adherence of cells to surfaces or to other cells.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Proteins prepared by recombinant DNA technology.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A member of the metalloproteinase family of enzymes that is principally responsible for cleaving FIBRILLAR COLLAGEN. It can degrade interstitial collagens, types I, II and III.
Elements of limited time intervals, contributing to particular results or situations.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
Methods for maintaining or growing CELLS in vitro.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains.
A process in which normal lung tissues are progressively replaced by FIBROBLASTS and COLLAGEN causing an irreversible loss of the ability to transfer oxygen into the bloodstream via PULMONARY ALVEOLI. Patients show progressive DYSPNEA finally resulting in death.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
A fibroblast growth factor that preferentially activates FIBROBLAST GROWTH FACTOR RECEPTOR 4. It was initially identified as an androgen-induced growth factor and plays a role in regulating growth of human BREAST NEOPLASMS and PROSTATIC NEOPLASMS.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A naturally occurring phenomenon where terminally differentiated cells dedifferentiate to the point where they can switch CELL LINEAGES. The cells then differentiate into other cell types.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Cellular signaling in which a factor secreted by a cell affects other cells in the local environment. This term is often used to denote the action of INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS on surrounding cells.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC
The sum of the weight of all the atoms in a molecule.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
A group of inherited metabolic diseases characterized by the accumulation of excessive amounts of acid mucopolysaccharides, sphingolipids, and/or glycolipids in visceral and mesenchymal cells. Abnormal amounts of sphingolipids or glycolipids are present in neural tissue. INTELLECTUAL DISABILITY and skeletal changes, most notably dysostosis multiplex, occur frequently. (From Joynt, Clinical Neurology, 1992, Ch56, pp36-7)
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A class of cellular receptors that have an intrinsic PROTEIN-TYROSINE KINASE activity.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It is found in hypertrophic CHONDROCYTES where it may play a role in CHONDROGENESIS and endochondral ossification.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
The functions of the skin in the human and animal body. It includes the pigmentation of the skin.
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Transport proteins that carry specific substances in the blood or across cell membranes.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Glycoproteins which have a very high polysaccharide content.
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
A group of cells that includes FIBROBLASTS, cartilage cells, ADIPOCYTES, smooth muscle cells, and bone cells.
Enzymes that catalyze the degradation of collagen by acting on the peptide bonds.
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
Bony cavity that holds the eyeball and its associated tissues and appendages.
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
A cell line derived from cultured tumor cells.
The most common and most biologically active of the mammalian prostaglandins. It exhibits most biological activities characteristic of prostaglandins and has been used extensively as an oxytocic agent. The compound also displays a protective effect on the intestinal mucosa.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
The chromosomal constitution of cells, in which each type of CHROMOSOME is represented twice. Symbol: 2N or 2X.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Group of alpharetroviruses (ALPHARETROVIRUS) producing sarcomata and other tumors in chickens and other fowl and also in pigeons, ducks, and RATS.
The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.
Arrest of cell locomotion or cell division when two cells come into contact.
An extracellular endopeptidase of vertebrate tissues similar to MATRIX METALLOPROTEINASE 1. It digests PROTEOGLYCAN; FIBRONECTIN; COLLAGEN types III, IV, V, and IX, and activates procollagenase. (Enzyme Nomenclature, 1992)
The fibrous tissue that replaces normal tissue during the process of WOUND HEALING.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A complex of related glycopeptide antibiotics from Streptomyces verticillus consisting of bleomycin A2 and B2. It inhibits DNA metabolism and is used as an antineoplastic, especially for solid tumors.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
A fibrillar collagen consisting of three identical alpha1(III) chains that is widely distributed in many tissues containing COLLAGEN TYPE I. It is particularly abundant in BLOOD VESSELS and may play a role in tissues with elastic characteristics.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
The process by which a DNA molecule is duplicated.
A receptor-regulated smad protein that undergoes PHOSPHORYLATION by ACTIVIN RECEPTORS, TYPE I. Activated Smad3 can bind directly to DNA, and it regulates TRANSFORMING GROWTH FACTOR BETA and ACTIVIN signaling.
A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
A fibroblast growth factor that is expressed primarily during development.
The double-layered skin fold that covers the GLANS PENIS, the head of the penis.
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
Cellular DNA-binding proteins encoded by the sis gene (GENES, SIS). c-sis proteins make up the B chain of PLATELET-DERIVED GROWTH FACTOR. Overexpression of c-sis causes tumorigenesis.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
The fibrous CONNECTIVE TISSUE surrounding the TOOTH ROOT, separating it from and attaching it to the alveolar bone (ALVEOLAR PROCESS).
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The quality of surface form or outline of CELLS.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A cell line established in 1962 from disaggregated Swiss albino mouse embryos. This fibroblast cell line is extremely popular in research.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
A 44-kDa extracellular signal-regulated MAP kinase that may play a role the initiation and regulation of MEIOSIS; MITOSIS; and postmitotic functions in differentiated cells. It phosphorylates a number of TRANSCRIPTION FACTORS; and MICROTUBULE-ASSOCIATED PROTEINS.
Retrovirus-associated DNA sequences (src) originally isolated from the Rous sarcoma virus (RSV). The proto-oncogene src (c-src) codes for a protein that is a member of the tyrosine kinase family and was the first proto-oncogene identified in the human genome. The human c-src gene is located at 20q12-13 on the long arm of chromosome 20.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.
A vascular connective tissue formed on the surface of a healing wound, ulcer, or inflamed tissue. It consists of new capillaries and an infiltrate containing lymphoid cells, macrophages, and plasma cells.
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.

Glycopeptides from the surgace of human neuroblastoma cells. (1/31038)

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (2/31038)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Lymphocyte proliferation inhibitory factor (PIF) in alcoholic liver disease. (3/31038)

Lymphocyte proliferation inhibitory factor (PIF) was determined in the supernatants of PHA-stimulated lymphocytes from patients with alcoholic liver disease. PIF was assayed by determining inhibition of DNA synthesis in WI-38 human lung fibroblasts. A two-fold greater inhibition in thymidine incorporation into DNA by lung fibroblasts was observed in supernatants of PHA stimulated lymphocytes from patients with alcoholic hepatitis or active Laennec's cirrhosis as compared with that found in control subjects or patients with fatty liver. It is suggested that decreased liver cell regeneration seen in some patients with alcoholic hepatitis may be due to increased elaboration of PIF.  (+info)

CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (4/31038)

Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors.  (+info)

Concomitant activation of pathways downstream of Grb2 and PI 3-kinase is required for MET-mediated metastasis. (5/31038)

The Met tyrosine kinase - the HGF receptor - induces cell transformation and metastasis when constitutively activated. Met signaling is mediated by phosphorylation of two carboxy-terminal tyrosines which act as docking sites for a number of SH2-containing molecules. These include Grb2 and p85 which couple the receptor, respectively, with Ras and PI 3-kinase. We previously showed that a Met mutant designed to obtain preferential coupling with Grb2 (Met2xGrb2) is permissive for motility, increases transformation, but - surprisingly - is impaired in causing invasion and metastasis. In this work we used Met mutants optimized for binding either p85 alone (Met2xPI3K) or p85 and Grb2 (MetPI3K/Grb2) to evaluate the relative importance of Ras and PI 3-kinase as downstream effectors of Met. Met2xPI3K was competent in eliciting motility, but not transformation, invasion, or metastasis. Conversely, MetP13K/Grb2 induced motility, transformation, invasion and metastasis as efficiently as wild type Met. Furthermore, the expression of constitutively active PI 3-kinase in cells transformed by the Met2xGrb2 mutant, fully rescued their ability to invade and metastasize. These data point to a central role for PI 3-kinase in Met-mediated invasiveness, and indicate that simultaneous activation of Ras and PI 3-kinase is required to unleash the Met metastatic potential.  (+info)

Differential stability of the DNA-activated protein kinase catalytic subunit mRNA in human glioma cells. (6/31038)

DNA-dependent protein kinase (DNA-PK) functions in double-strand break repair and immunoglobulin [V(D)J] recombination. We previously established a radiation-sensitive human cell line, M059J, derived from a malignant glioma, which lacks the catalytic subunit (DNA-PKcs) of the DNA-PK multiprotein complex. Although previous Northern blot analysis failed to detect the DNA-PKcs transcript in these cells, we show here through quantitative studies that the transcript is present, albeit at greatly reduced (approximately 20x) levels. Sequencing revealed no genetic alteration in either the promoter region, the kinase domain, or the 3' untranslated region of the DNA-PKcs gene to account for the reduced transcript levels. Nuclear run-on transcription assays indicated that the rate of DNA-PKcs transcription in M059J and DNA-PKcs proficient cell lines was similar, but the stability of the DNA-PKcs message in the M059J cell line was drastically (approximately 20x) reduced. Furthermore, M059J cells lack an alternately spliced DNA-PKcs transcript that accounts for a minor (5-20%) proportion of the DNA-PKcs message in all other cell lines tested. Thus, alterations in DNA-PKcs mRNA stability and/or the lack of the alternate mRNA may result in the loss of DNA-PKcs activity. This finding has important implications as DNA-PKcs activity is essential to cells repairing damage induced by radiation or radiomimetric agents.  (+info)

Signals from the Ras, Rac, and Rho GTPases converge on the Pak protein kinase in Rat-1 fibroblasts. (7/31038)

Ras plays a key role in regulating cellular proliferation, differentiation, and transformation. Raf is the major effector of Ras in the Ras > Raf > Mek > extracellular signal-activated kinase (ERK) cascade. A second effector is phosphoinositide 3-OH kinase (PI 3-kinase), which, in turn, activates the small G protein Rac. Rac also has multiple effectors, one of which is the serine threonine kinase Pak (p65(Pak)). Here we show that Ras, but not Raf, activates Pak1 in cotransfection assays of Rat-1 cells but not NIH 3T3 cells. We tested agents that activate or block specific components downstream of Ras and demonstrate a Ras > PI 3-kinase > Rac/Cdc42 > Pak signal. Although these studies suggest that the signal from Ras through PI 3-kinase is sufficient to activate Pak, additional studies suggested that other effectors contribute to Pak activation. RasV12S35 and RasV12G37, two effector mutant proteins which fail to activate PI 3-kinase, did not activate Pak when tested alone but activated Pak when they were cotransfected. Similarly, RacV12H40, an effector mutant that does not bind Pak, and Rho both cooperated with Raf to activate Pak. A dominant negative Rho mutant also inhibited Ras activation of Pak. All combinations of Rac/Raf and Ras/Raf and Rho/Raf effector mutants that transform cells cooperatively stimulated ERK. Cooperation was Pak dependent, since all combinations were inhibited by kinase-deficient Pak mutants in both transformation assays and ERK activation assays. These data suggest that other Ras effectors can collaborate with PI 3-kinase and with each other to activate Pak. Furthermore, the strong correlation between Pak activation and cooperative transformation suggests that Pak activation is necessary, although not sufficient, for cooperative transformation of Rat-1 fibroblasts by Ras, Rac, and Rho.  (+info)

Differential roles for cyclin-dependent kinase inhibitors p21 and p16 in the mechanisms of senescence and differentiation in human fibroblasts. (8/31038)

The irreversible G1 arrest in senescent human diploid fibroblasts is probably caused by inactivation of the G1 cyclin-cyclin-dependent kinase (Cdk) complexes responsible for phosphorylation of the retinoblastoma protein (pRb). We show that the Cdk inhibitor p21(Sdi1,Cip1,Waf1), which accumulates progressively in aging cells, binds to and inactivates all cyclin E-Cdk2 complexes in senescent cells, whereas in young cells only p21-free Cdk2 complexes are active. Furthermore, the senescent-cell-cycle arrest occurs prior to the accumulation of the Cdk4-Cdk6 inhibitor p16(Ink4a), suggesting that p21 may be sufficient for this event. Accordingly, cyclin D1-associated phosphorylation of pRb at Ser-780 is lacking even in newly senescent fibroblasts that have a low amount of p16. Instead, the cyclin D1-Cdk4 and cyclin D1-Cdk6 complexes in these cells are associated with an increased amount of p21, suggesting that p21 may be responsible for inactivation of both cyclin E- and cyclin D1-associated kinase activity at the early stage of senescence. Moreover, even in the late stage of senescence when p16 is high, cyclin D1-Cdk4 complexes are persistent, albeit reduced by +info)

Rat ES cells were derived using 3I medium from E4.5 blastocysts. Rat embryonic fibroblast cells were derived form E14.5 embryos. To analyze the mechanism under the selfrenewal of rat ES cells, microarrays were used for the genome wide analysis of gene expressoin profiles in rat ES cells. Rat embryonic fibroblast cells and mouse ES cells were tested at same time as control. Our results from clustering analysis demonstrated that the gene expression profile of rat ES cells resembles mouse ES cells, but not REFs. Keyword: 3I medium; rat embryonic stem cells; mouse ES cells; rat embryonic fibroblast cells Rat ES cells were cultured in 3I medium; rat embryonic fibroblast cells were derived and cultured GMEM/10% FBS; mouse ES cells (C57/BL6)were cultured in GMEM/10% FBS added LIF and feeder cells were removed before RNA extraction. Three replicates each.
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TY - JOUR. T1 - Retinoic acid restores normal growth control to a transformed mouse embryo fibroblast cell line. AU - Levine, Alan E.. AU - Crandall, Craig A.. AU - Brattain, Diane. AU - Chakrabarty, Subhas. AU - Brattain, Michael G.. PY - 1986/10. Y1 - 1986/10. N2 - The effects of retinoic acid on a transformed mouse embryo fibroblast cell line (AKR-MCA) were examined. Treatment with retinoic acid restored a non-transformed phenotype to this transformed cell line in a dose dependent manner. Retinoic acid (RA) treated AKR-MCA cells showed a non-transformed morphology, a slower growth rate, and did not grow with anchorage independence. A 38,000 Da protein was phosphorylated to a high degree in the AKR-MCA transformed cell line compared to the non-transformed AKR-2B cell line. RA treatment greatly reduced the level of phosphorylation of this protein in AKR-MCA cells. Growth arrested AKR-MCA cells showed a mitogenic response to nutrient replenishment, but not to epidermal growth factor (EGF). ...
Murine studies have shown that immunologic targeting of the tumor vasculature, a key element of the tumor stroma, can lead to protective immunity in the absence of significant pathology. In the current study, we expand the scope of stroma-targeted immunotherapy to antigens expressed in tumor-associated fibroblasts, the predominant component of the stroma in most types of cancer. Mice were immunized against fibroblast activation protein (FAP), a product up-regulated in tumor-associated fibroblasts, using dendritic cells transfected with FAP mRNA. Using melanoma, carcinoma, and lymphoma models, we show that tumor growth was inhibited in tumor-bearing mice vaccinated against FAP and that the magnitude of the antitumor response was comparable to that of vaccination against tumor cell-expressed antigens. Both s.c. implanted tumors and lung metastases were susceptible to anti-FAP immunotherapy. The antitumor response could be further enhanced by augmenting the CD4+ T-cell arm of the anti-FAP immune ...
It is well accepted that there is an increase in the number of fibroblasts in the airways of patients with asthma that correlates with thickness of lamina reticularis and disease severity. Moreover, fibroblast activation and differentiation to myofibroblasts are also evident [1-4].. In the present study, we aimed to investigate the in vitro effect of glucocorticosteroids and short-acting β2-agonists widely used as first-line antiasthmatic drugs on human lung fibroblast proliferation and IL-6 production. We specifically choose to evaluate fibroblast proliferation because this is the first hallmark of fibrosis taking place. IL-6 was selected among a plethora of proinflammatory profibrotic cytokines produced by the fibroblast[22] that mainly influences the inflammatory response [23, 24].. We found that dexamethasone and salbutamol alone and in combination increase both human fetal lung and human bronchial fibroblast proliferation. Moreover, we demonstrate for the first time that when the ...
Title: Genetic characterization of skin fibroblast cell lines by DNA fingerprinting. Authors: NK Nagpal, BK Beniwal, GC Gahlot and SC Gupta. Source: Ruminant Science (2016)-5(2):149-158.. Cite this reference as: Nagpal NK, Beniwal BK, Gahlot GC and Gupta SC (2016). Genetic characterization of skin fibroblast cell lines by DNA fingerprinting. Ruminant Science 5(2):149-158.. Abstract. Present study was carried out for genetic characterization of Bhadawari buffalo skin fibroblast cell lines using ten microsatellite primers. The primary cells explants from tissue of six cell lines MF-22, MF-37 and MF-82 (females) and MM-31, MM-32 and MM-79 (males) collected from 6-8 months old calves of Bhadawari buffalo breed were employed for development of cell lines. To establish the integrity of cell line which may have lost due to improper passaging, sharing of cultural media or inaccurate labeling, DNA fingerprinting was exercised. The PCR and denaturing sequence gel electrophoresis were carried out for ...
Title: Genetic characterization of skin fibroblast cell lines by DNA fingerprinting. Authors: NK Nagpal, BK Beniwal, GC Gahlot and SC Gupta. Source: Ruminant Science (2016)-5(2):149-158.. Cite this reference as: Nagpal NK, Beniwal BK, Gahlot GC and Gupta SC (2016). Genetic characterization of skin fibroblast cell lines by DNA fingerprinting. Ruminant Science 5(2):149-158.. Abstract. Present study was carried out for genetic characterization of Bhadawari buffalo skin fibroblast cell lines using ten microsatellite primers. The primary cells explants from tissue of six cell lines MF-22, MF-37 and MF-82 (females) and MM-31, MM-32 and MM-79 (males) collected from 6-8 months old calves of Bhadawari buffalo breed were employed for development of cell lines. To establish the integrity of cell line which may have lost due to improper passaging, sharing of cultural media or inaccurate labeling, DNA fingerprinting was exercised. The PCR and denaturing sequence gel electrophoresis were carried out for ...
TY - JOUR. T1 - PFG acted as an inducer of premature senescence in TIG-1 normal diploid fibroblast and an inhibitor of mitosis in the HeLa cells. AU - Huang, Ying. AU - Ohno, Osamu. AU - Miyamoto, Kenji. PY - 2019/6/1. Y1 - 2019/6/1. N2 - Our previous work has reported an anti-proliferative compound from moutan cortex, paeoniflorigenone which can induce cancer-selective apoptosis. However, its anti-proliferative mechanism is still unknown. According to morphology changes (hypertrophy and flattening), we hypothesized that PFG can induce senescence or inhibit cell mitosis. Here we show that PFG can induce cellular senescence, evidenced by the expression of senescence-associated β-galactosidase, G0/G1 cell cycle arrest and permanent loss of proliferative ability, in normal TIG-1 diploid fibroblast but not cancerous HeLa cells. In cancerous HeLa cells, PFG inhibited proliferation by inducing S and G2/M cell cycle arrest and mitosis inhibition. DNA damage response was activated by PFG, interestingly ...
Fibroblasts play important roles in several cancers. It was hypothesized that cholangiocarcinoma (CCA)-associated fibroblasts (Cfs) differ from non-tumorigenic liver fibroblasts (Lfs) in their gene expression profiles resulting in the capability to promote cancer. Periostin (PN) is a multi-functional protein and has emerged as a promising marker for tumor progression. The role of PN in CCA, however, has not yet been explored. In this study, the gene expression profile of Cfs in comparison to Lfs was performed using oligonucleotide microarrays. The common- and unique-expressed genes in Cfs and the promising roles in cancer promotion and progression were determined. PN was markedly over-expressed in Cfs confirmed by real time RT-PCR and western blot analysis. Immunohistochemistry examination of a number of patients with intrahepatic CCA showed the expression of PN solely in stromal fibroblasts, but was expressed neither in cancer cells nor immune cells. Low to no expression of PN was observed in tissues
In our laboratory, recent single cell electrophysiologic studies have demonstrated the absence of voltage-gated Ca2+ channels in human cardiac fibroblasts. The more positive membrane potential found in these cells suggests that Ca2+ entry occurs through a different mechanism. We hypothesized that non-voltage-gated Ca2+-permeable TRP channels are responsible for Ca2+ entry in human cardiac fibroblasts. With informed consent, right atrial biopsies were obtained from patients undergoing cardiac surgery (n=4:.3M, 1F; mean age 65±8 yrs, EF 63±5%, LVEDP 24±4 mm Hg). Fibroblasts were dissociated and cultured for 7 to 10 days. We found that TRPC1, TRPC4, TRPC6, TRPV4, TRPV5, TRPV6, TRPM4 and TRPM7 were detectable at message levels by RT-PCR. Functional expression of these channels was evaluated by patch-clamp technique. An outward rectifying current with typical I-V relation of TRPM7 was readily recorded in the fibroblasts. The averaged current density was 14.5±0.8 pA/pF (mean±SEM, n=60 from four ...
TY - JOUR. T1 - Protective effect of resveratrol against caspase 3 activation in primary mouse fibroblasts. AU - Ulakcsai, Zsófia. AU - Bagaméry, Fruzsina. AU - Vincze, István. AU - Szöko, Éva. AU - Tábi, Tamás. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Aim: To study the effect of resveratrol on survival and caspase 3 activation in non-transformed cells after serum deprivation. Methods: Apoptosis was induced by serum deprivation in primary mouse embryonic fibroblasts. Caspase 3 activation and lactate dehydrogenase release were assayed as cell viability measure by using their fluorogenic substrates. The involvement of PI3K, ERK, JNK, p38, and SIRT1 signaling pathways was also examined. Results: Serum deprivation of primary fibroblasts induced significant activation of caspase 3 within 3 hours and reduced cell viability after 24 hours. Resveratrol dose-dependently prevented caspase activation and improved cell viability with 50% inhibitory concentration (IC50) = 66.3 ± 13.81 μM. It also reduced ...
TY - JOUR. T1 - Altered transcriptome signature of phenotypically normal skin fibroblasts heterozygous for CDKN2A in familial melanoma. T2 - Relevance to early intervention. AU - Fan, Meiyun. AU - Pfeffer, Susan R.. AU - Lynch, Henry T.. AU - Cassidy, Pamela. AU - Leachman, Sancy. AU - Pfeffer, Lawrence M.. AU - Kopelovich, Levy. PY - 2013. Y1 - 2013. N2 - Familial melanoma (FM) is a dominantly heritable cancer that is associated with mutations in the tumor suppressor CDKN2A/p16. In FM, a single inherited hit occurs in every somatic cell, enabling interrogation of cultured normal skin fibroblasts (SFs) from FM gene carriers as surrogates for the cell of tumor origin, namely the melanocyte.We compared the gene expression profile of SFs from FM individuals with two distinct CDKN2A/p16 mutations (V126D-p16 and R87P-p16) with the gene expression profile of SFs from age-matched individuals without p16 mutations and with no family history of melanoma. We show an altered transcriptome signature in ...
p,Fibroblasts play a major role in heart physiology. They are at the origin of the extracellular matrix renewal and production of various paracrine and autocrine factors. In pathological conditions, fibroblasts proliferate, migrate and differentiate into myofibroblasts leading to cardiac fibrosis. This differentiated status is associated with changes in expression profile leading to neo-expression of proteins such as ionic channels. The present study investigates further electrophysiological changes associated with fibroblast differentiation focusing on the activity of voltage-gated sodium channels in human atrial fibroblasts and myofibroblasts. Using the patch clamp technique we show that human atrial myofibroblasts display a fast inward voltage gated sodium current with a density of 13.28 ± 2.88 pA pF(-1) whereas no current was detectable in non-differentiated fibroblasts. Quantitative RT-PCR reveals a large amount of transcripts encoding the Na(v)1.5 α-subunit with a fourfold increased ...
Human fibroblasts include: bladder, cardiac, dermal, gingival, lung-airway, prostate, scleral, uterine, and vas deferens.. Lifeline® normal Human Fibroblasts provide an ideal cell system to study wound healing, toxicology, cancer, or basic cell biology in various organs including skin, lung, bladder, and the reproductive systems. Our normal Human Fibroblasts can also be used for drug screening, drug development, and genome editing applications. Additionally, our fibroblast lines are ideal for establishing serum free human feeder layers for human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and other cell culture applications requiring feeder layers.. Human Dermal Fibroblasts (Adult or Neonatal) and Xeno-Free Human Dermal Fibroblasts are cryopreserved as primary cells ...
Administration of selected concentrations of ebselen and N-acetyl cysteine have been proven to display an antioxidant potential based on their effect on markers of T cell integrity and function in human peripheral blood mononuclear cells and CD4+ T cell clones. Here we assessed the impact of various antioxidant concentrations on replicative aging of primary human fibroblast strains derived from embryonic lung (MRC-5) and foreskin (HFF). None of the antioxidant concentrations affected the cumulative population doublings, levels of oxidative DNA damage, intracellular GSH:GSSG ratio, potency of heat shock responses and the induction of senescence in both fibroblast strains. Our results showed no effect of both antioxidants on primary fibroblast strains and reveal their cell type specific antioxidant potential.
TY - JOUR. T1 - The connexin mimetic peptide Gap27 increases human dermal fibroblast migration in hyperglycemic and hyperinsulinemic conditions in vitro. AU - Wright, Catherine. AU - Pollok, Simone AU - Flint, David J.. AU - Brandner, Johanna M.. AU - Martin, Patricia. N1 - publisher version of article not permitted for display in repositories (ET 18-10-13). PY - 2012/1. Y1 - 2012/1. N2 - Significant increases in skin wound healing rates occur by reducing connexin-mediated communication (CMC). Gap27, a connexin (Cx) mimetic peptide targeted to the second extracellular loop of Cx43, which inhibits CMC, increases migration of human keratinocytes and dermal fibroblasts. To examine the efficacy of Gap27 in a hyperglycemic and hyperinsulinemic in vitro environment, cell migration, gap junction, and Cx hemichannel functionality and cell-substrate adhesion assays were performed on human dermal fibroblasts and diabetic fibroblast and keratinocytes. AB - Significant increases in skin wound healing rates ...
Human fibroblasts can express and transport both PC-I aggregates and VSVG through the Golgi complex. Human fibroblasts were stimulated to synthesize PC-I and in
Applied mechanical forces, such as those resulting from fluid flow, trigger cells to change their functional behavior or phenotype. However, there is little known about how fluid flow affects fibroblasts. The hypothesis of this thesis is that dermal fibroblasts undergo significant changes of expression of differentiation genes after exposure to fluid flow (or shear stress). To test the hypothesis, human dermal fibroblasts were exposed to laminar steady fluid flow for 20 and 40 hours and RNA was collected for microarray analysis. Gene expression data was processed using gene network analysis, pathway analysis, and gene functional analysis with comparison to data from publicly available data sets. Additional treatment with PI3K/mTOR pathway inhibitor, PI-103, was performed to evaluate pathway involvement in flow modulation of gene expression. Results from overall transcription analysis demonstrated that fluid flow modulated many genes in fibroblasts including those related to differentiation, ...
Helen There is a marker called prolyl-4-hydroxylase that is supposed to react with fibroblasts. Its from Acris cat number AF5110-1. The antibody is a mouse anti-rat. It is supposed to work in FFPE material. I just received it and have not had a change to start working on it, but I can update as soon as I start working with it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 [email protected] Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Helen Ilsley Sent: Monday, July 17, 2006 3:15 AM To: [email protected] Subject: [Histonet] fibroblast marker Hi I wonder if anyone can help me. I am looking for a fibroblast marker which can cross react with any of the following: ...
Collagen type I production decreases with aging, leading to wrinkles and impaired skin function. Prostaglandin E2 (PGE2), a lipid-derived signaling molecule produced from arachidonic acid by cyclo-oxygenase, inhibits collagen production and induces matrix metallopeptidase 1 (MMP1) expression by fibroblasts in vitro. PGE2-induced collagen expression inhibition and MMP1 promotion are aging mechanisms. This study investigated the role of E-prostanoid 1 (EP1) in PGE2 signaling in normal human dermal fibroblasts (NHDFs). When EP1 expression was inhibited by EP1 small interfering RNA (siRNA), there were no significant changes in messenger RNA (mRNA) levels of collagen, type I, alpha 1 (COL1A1)/MMP1 between siRNA-transfected NHDFs and siRNA-transfected NHDFs with PGE2. This result showed that EP1 is a PGE2 receptor. Extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation after PGE2 treatment significantly increased by ~2.5 times. In addition, PGE2 treatment increased the intracellular Ca2+
Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive fibrotic lung disease for which there is no cure. Current therapeutics are only able to slow disease progression, therefore there is a need to explore alternative, novel treatment options. There is increasing evidence that the 3′, 5′ cyclic adenosine monophosphate (cAMP) pathway is an important modulator in the development of fibrosis, with increasing levels of cAMP able to inhibit cellular processes associated with IPF. In this study we investigate the expression of Gs-coupled G protein-coupled receptors (GPCR) on human lung fibroblasts (HLF), and explore which can increase cAMP levels, and are most efficacious at inhibiting proliferation and differentiation. Using TaqMan arrays we determined that fibroblasts express a range of Gs-coupled GPCR. The function of selected agonists at expressed receptors was then tested in a cAMP assay, and for their ability to inhibit fibroblast proliferation and differentiation. Expression analysis of
The importance of the tumor microenvironment on cancer growth and invasion are well appreciated (1-3), and the stiffness of the tumor stroma has been shown to drive tumorigenesis and invasion (8-10). However, in addition to matrix stiffness, interstitial flow is an important mechanical stress in the tumor stroma (5). By examining the interplay between tumor cells, fibroblasts, and interstitial flow, we showed that flow guides fibroblast invasion, leading to concurrent invasion of MDA-MB-435S tumor cells through the ECM. Without interstitial flow, fibroblasts did not affect tumor cell invasion.. TGF-β1 regulates a variety of tumor suppressive and promoting effects, including epithelial homeostasis, epithelial-to-mesenchymal transition, myofibroblast differentiation, and metastasis (36). TGF-β1 was necessary for interstitial flow-enhanced fibroblast invasion (Fig. 2A) but only indirectly involved in tumor cell invasion (Fig. 2D). We hypothesize that TGF-β1 may increase fibroblast invasion ...
TY - JOUR. T1 - c-Src enhances the spreading of src-/-fibroblasts on fibronectin by a kinase-independent mechanism. AU - Kaplan, Kenneth B.. AU - Swedlow, Jason R.. AU - Morgan, David O.. AU - Varmus, Harold E.. PY - 1995/6/15. Y1 - 1995/6/15. N2 - We have explored the role of the tyrosine kinase c-Src in cellular adhesion. Fibroblasts derived from src-/-mice (src-/-fibroblasts) exhibit a reduced rate of spreading on fibronectin. This defect is rescued by expression of wild-type chicken c-Src. Analyses of mutants suggest that c-Src increases the rate of cell spreading in src-/- fibroblasts through a kinase-independent mechanism requiring both the SH3 and SH2 domains. To further address the role of c-Src in adhesion, we examined the activity and subcellular distribution of c-Src during the adhesion of fibroblasts on fibronectin. We observed a transient increase in the specific kinase activity of c-Src accompanied by the partial dephosphorylation of the negative regulatory site Y527. Activation of ...
Identification of fibroblast derived factors in tumor progression has the potential to provide novel molecular targets for modulating tumor cell growth and metastasis. Multiple matrix metalloproteases (MMPs) are expressed by both mesenchymal and epithelial cells within head and neck squamous cell carcinomas (HNSCCs), but the relative importance of these enzymes and the cell source is the subject of controversy. The invasive potential of HNSCC tumor cells were assessed in vitro atop type I collagen gels in coculture with wild-type (WT), MMP-2 null, MMP-9 null or MT1-MMP null fibroblasts. A floor of mouth mouse model of HNSCC was used to assess in vivo growth after co-injection of FaDu tumor cells with MMP null fibroblasts. Here we report changes in tumor phenotype when FaDu HNSCCs cells are cocultured with WT, MMP-2 null, MMP-9 null or MT1-MMP null fibroblasts in vitro and in vivo. WT, MMP-2 null and MMP-9 null fibroblasts, but not MT1-MMP null fibroblasts, spontaneously invaded into type I collagen gels
Identification of fibroblast derived factors in tumor progression has the potential to provide novel molecular targets for modulating tumor cell growth and metastasis. Multiple matrix metalloproteases (MMPs) are expressed by both mesenchymal and epithelial cells within head and neck squamous cell carcinomas (HNSCCs), but the relative importance of these enzymes and the cell source is the subject of controversy. The invasive potential of HNSCC tumor cells were assessed in vitro atop type I collagen gels in coculture with wild-type (WT), MMP-2 null, MMP-9 null or MT1-MMP null fibroblasts. A floor of mouth mouse model of HNSCC was used to assess in vivo growth after co-injection of FaDu tumor cells with MMP null fibroblasts. Here we report changes in tumor phenotype when FaDu HNSCCs cells are cocultured with WT, MMP-2 null, MMP-9 null or MT1-MMP null fibroblasts in vitro and in vivo. WT, MMP-2 null and MMP-9 null fibroblasts, but not MT1-MMP null fibroblasts, spontaneously invaded into type I collagen gels
Fibroblast apoptosis is a critical component of normal repair and the acquisition of an apoptosis-resistant phenotype contributes to the pathogenesis of fibrotic repair. Fibroblasts from fibrotic lungs of humans and mice demonstrate resistance to apoptosis induced by Fas-ligand and prior studies have shown that susceptibility to apoptosis is enhanced when Fas (CD95) expression is increased in these cells. Moreover, prior work shows that Fas expression in fibrotic lung fibroblasts is reduced by epigenetic silencing of the Fas promoter. However, the mechanisms by which microenvironmental stimuli such as TGF-β1 and substrate stiffness affect fibroblast Fas expression are not well understood. Primary normal human lung fibroblasts (IMR-90) were cultured on tissue culture plastic or on polyacrylamide hydrogels with Youngs moduli to recapitulate the compliance of normal (400 Pa) or fibrotic (6400 Pa) lung tissue and treated with or without TGF-β1 (10 ng/mL) in the presence or absence of protein kinase
Wound healing is a complex process that requires an interplay between several cell types. Classically, fibroblasts have been viewed as producers of extracellular matrix, but more recently they have been recognized as orchestrators of the healing response, promoting and directing, inflammation and neovascularization processes. Compared to those from healthy tissue, inflammation-associated fibroblasts display a dramatically altered phenotype and have been described as sentinel cells, able to switch to an immunoregulatory profile on cue. However, the activation mechanism still remains largely uncharacterized. Nemosis is a model for stromal fibroblast activation. When normal human primary fibroblasts are deprived of growth support they cluster, forming multicellular spheroids. Clustering results in upregulation of proinflammatory markers such as cyclooxygenase-2 and secretion of prostaglandins, proteinases, cytokines, and growth factors. Fibroblasts in nemosis induce wound healing and tumorigenic ...
Human Pulmonary Fibroblast Cell Pellet ...
Cardiac fibrosis is a major component of heart disease and is a hallmark of decreased cardiac function. Currently, there are no treatments that attenuate fibrosis directly. This major hurdle can be overcome by targeting the resident fibroblast. Preliminary data demonstrates that loss of PDGFRα expression in the adult cardiac fibroblast lineage results in loss of over half of resident fibroblasts. A time course experiment revealed that in as little as 4 days after PDGFRα gene deletion fibroblast loss can observed. Based on the basal level of fibroblast proliferation (0.8%+/-0.9, i.e. 4 of 398 cells), we hypothesize that PDGFRα signaling is essential for fibroblast maintenance and that fibroblasts undergo rapid turnover. We have begun to elucidate which downstream signals of PDGFRα are involved the different roles of the fibroblast. Using a PDGFRα-dependent-PI3K-deficient mouse model, preliminary data indicates that PDGFRα-dependent PI3K signaling is involved in this cell survival response. ...
Cancer-associated fibroblasts (CAF) have been reported to support tumor progression by a variety of mechanisms. However, their role in the progression of non-small cell lung cancer (NSCLC) remains poorly defined. In addition, the extent to which specific proteins secreted by CAFs contribute directly to tumor growth is unclear. To study the role of CAFs in NSCLCs, a cross-species functional characterization of mouse and human lung CAFs was conducted. CAFs supported the growth of lung cancer cells in vivo by secretion of soluble factors that directly stimulate the growth of tumor cells. Gene expression analysis comparing normal mouse lung fibroblasts and mouse lung CAFs identified multiple genes that correlate with the CAF phenotype. A gene signature of secreted genes upregulated in CAFs was an independent marker of poor survival in patients with NSCLC. This secreted gene signature was upregulated in normal lung fibroblasts after long-term exposure to tumor cells, showing that lung fibroblasts are ...
Chronic kidney disease (CKD) is a global socioeconomic problem. It is characterised by the presence of differentiated myofibroblasts that, in response to TGF B-1, produce tissue fibrosis, leading to renal failure. Here we define a novel interaction between the SET9 lysine methyltransferase and SMAD3, the principle mediator of TGF B-1 signalling in myofibroblasts. We show that SET9 deficient fibroblasts exhibit globally altered gene expression profiles in response to TGF B-1, whilst overexpression of SET9 enhances SMAD3 transcriptional activity. We also show that SET9 facilitates SMAD3 nuclear import and controls SMAD3 protein degradation, in a manner involving ubiquitination. On a cellular level, we demonstrate that SET9 is broadly required for TGF B-1 effects in diseased primary renal fibroblasts; SET9 promotes fibroblast migration into wounds, expression of extracellular matrix proteins, collagen contractility and myofibroblast differentiation. Finally, we demonstrate that SET9 is recruited to ...
We performed subtractive and differential hybridization for transcript comparison between murine fibroblasts and isogenic epithelium, and observed only a few novel intracellular genes which were relatively specific for fibroblasts. One such gene encodes a filament-associated, calcium-binding protein, fibroblast-specific protein 1 (FSP1). The promoter/enhancer region driving this gene is active in fibroblasts but not in epithelium, mesangial cells or embryonic endoderm. During development, FSP1 is first detected by in situ hybridization after day 8.5 as a postgastrulation event, and is associated with cells of mesenchymal origin or of fibroblastic phenotype. Polyclonal antiserum raised to recombinant FSP1 protein stained the cytoplasm of fibroblasts, but not epithelium. Only occasional cells stain with specific anti-FSP1 antibodies in normal parenchymal tissue. However, in kidneys fibrosing from persistent inflammation, many fibroblasts could be identified in interstitial sites of collagen ...
Human Pulmonary Fibroblast MicroRNA ...
Resident fibroblasts synthesize the cardiac extracellular matrix, and can undergo phenotype conversion to myofibroblasts to augment matrix production, impairing function and contributing to organ failure. A significant gap in our understanding of the transcriptional regulation of these processes exists. Given the key role of this phenotype conversion in fibrotic disease, the identification of such novel transcriptional regulators may yield new targets for therapies for fibrosis. Using explanted primary cardiac fibroblasts in gain- and loss-of-function studies, we found that scleraxis critically controls cardiac fibroblast/myofibroblast phenotype by direct transcriptional regulation of myriad genes that effectively define these cells, including extracellular matrix components and α-smooth muscle actin. Scleraxis furthermore potentiated the TGFβ/Smad3 signaling pathway, a key regulator of myofibroblast conversion, by facilitating transcription complex formation. While scleraxis promoted fibroblast to
Survivin encoded by BIRC5 belongs to the group of proteins that inhibit apoptosis. It consists of the BIR and α-helical C domains. In addition to its inhibitory activity, it plays an important role in cell cycle regulation. Adalimumab is an immunosuppressive drug, a recombinant human anti-TNF-α monoclonal antibody. It is used in the treatment of autoimmune diseases.The aim of the study was to evaluate changes in the expression of BIRC5 and genes encoding apoptosis inhibitors (IAP), depending on the exposure time of the cells to adalimumab. The study material consisted of normal human dermal fibroblasts (NHDF) cultured under standard conditions in the presence of adalimumab (8µg/mL) for 2, 8 and 24 hours. The expression profile of genes associated with apoptosis was determined with the use of HG-U133A 2.0 oligonucleotide microarrays (Affymetrix). The comparative analysis was performed with one-way ANOVA and Tukey's HSD tests (p,0.05) using the PL-Grid Infrastructure ...
The effect of serum deprivation on proliferating cells is well known, in contrast its role on primary cell cultures, at confluence, has not been deeply investigated. Therefore, in order to explore the response of quiescent cells to serum deprivation, ubiquitous mesenchymal cells, as normal human dermal fibroblasts, were grown, for 48 h after confluence, in the presence or absence of 10% FBS. Fibroblast behaviour (i.e. cell morphology, cell viability, ROS production and elastin synthesis) was evaluated morphologically and biochemically. Moreover, the protein profile was investigated by 2-DE and differentially expressed proteins were identified by MS. Serum withdrawal caused cell shrinkage but did not significantly modify the total cell number. ROS production, as evaluated by the dihydroethidium (DH2) probe, was increased after serum deprivation, whereas elastin synthesis, measured by a colorimetric method, was markedly reduced in the absence of serum. By proteome analysis, 41 proteins appeared to ...
Results We observed increased expression of JMJD3 in SSc skin compared to healthy controls. Fibroblast-specific overexpression of JMJD3 was also reflected in experimental fibrosis models. TGFβ upregulated JMJD3. Inhibition of JMJD3 increased H3K27me3 in vitro and in vivo. Inhibition of JMJD3 reverted the activated fibroblast phenotype in SSc fibroblasts and decreased the expression of contractile fibers and of α-smooth muscle actin. In addition, JMJD3 inhibition reduced the basal and TGFβ induced collagen secretion of SSc fibroblasts. JMJD3 regulated the TGFβ induced expression of Fra2. GSKJ4 reverted the TGFβ induced reduction of H3K27me3 at the Fra2 promotor. Moreover, the anti-fibrotic effects of JMJD3 inhibition were evened in Fra2 knockout fibroblasts. Overexpression of Fra2 in JMJD3-knockdown fibroblasts restored the profibrotic effect of JMJD3. In vivo, inhibition of JMJD3 ameliorated fibrosis in bleomycin- and TopoI- induced experimental fibrosis and reduced dermal thickening, ...
Cancer-associated fibroblasts (CAF) remain a poorly characterized, heterogeneous cell population. Here we characterized two previously described tumor-promoting CAF sub-types, smooth muscle actin (SMA)-positive myofibroblasts and senescent fibroblasts, identifying a novel link between the two. Analysis of CAF cultured ex vivo, showed that senescent CAF are predominantly SMA-positive; this was confirmed by immunochemistry in head & neck (HNSCC) and esophageal (EAC) cancers. In vitro, we found that fibroblasts induced to senesce develop molecular, ultrastructural and contractile features typical of myofibroblasts and this is dependent on canonical TGF-β signaling. Similar to TGF-β1-generated myofibroblasts, these cells secrete soluble factors that promote tumor cell motility. However, RNA-sequencing revealed significant transcriptomic differences between the two SMA-positive CAF groups, particularly in genes associated with extracellular matrix (ECM) deposition and organization, which
Both in vivo and in vitro studies have demonstrated that fibroblasts contribute to tumor formation and growth rates (6) , and can be thought of as contracted farmers used by tumors to prepare the microenvironment. Fibroblasts coinoculated with breast or bladder tumor cell lines in nude mice shorten tumor latency and increase tumor growth (7) . Fibroblasts cultured from malignant tumors have stimulatory effects on MCF-7 cells, whereas fibroblasts cultured from normal tissue are inhibitory (8) . Phenotypic differences among tumor-associated fibroblasts have also been seen. Fibroblasts with smooth muscle differentiation, termed myofibroblasts, are abundant in the stromal cells of malignant breast tissue but are rarely seen in normal breast tissue (9) . These findings suggest that tumor-associated fibroblasts are functionally distinct compared with fibroblasts that are not in the tumor microenvironment, and subpopulations of fibroblast may perform specialized functions to coordinate events ...
Idiopathic pulmonary fibrosis (IPF) is a progressive, severely debilitating disease with a high mortality rate. Nintedanib (BIBF 1120) is a receptor tyrosine kinase inhibitor specific for platelet-derived growth factor receptor, fibroblast growth factor receptor and vascular endothelial growth factor receptor. Its effect on IPF disease progression measured by lung function decline has been investigated in two replicate Phase III clinical trials (INPULSIS-1 and -2) in patients with IPF. Interleukin-1 beta (IL-1β) is a potent pro-fibrotic mediator stimulating fibroblast proliferation a hallmark of IPF.. Aim: To determine the effect of nintedanib on proliferation rate of IL-1β-stimulated primary human lung fibroblasts.. Methods: Primary human lung fibroblasts from patients with IPF (IPF-HPF) and from non-fibrotic control donors (HPF) were incubated with nintedanib (1 nM - 1000 nM) for 30 min. Subsequently the cells were stimulated with IL-1β and cell proliferation was assessed by BrdU assay ...
3T3 cells come from a cell line established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. The 3T3 cell line has become the standard fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. The 3T3 designation refers to the abbreviation of 3-day transfer, inoculum 7005300000000000000♠3×105 cells. This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called 3T3 protocol. The primary mouse embryonic fibroblast cells were transferred (the T) every 3 days (the first 3), and inoculated at the rigid density of 7005300000000000000♠3×105 cells per 20 cm2 dish (the second 3) continuously. The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named 3T3 cells. Specifically, ...
TY - JOUR. T1 - Foetal-to-adult transitions in fibroblast phenotype. T2 - their possible relevance to the pathogenesis of cancer. AU - Schor, S L. AU - Schor, A M. PY - 1987. Y1 - 1987. N2 - We have previously shown that the migration of foetal, adult and transformed fibroblasts into three-dimensional collagen gels is differentially affected by plating cell density. We now present data indicating that the migration of these fibroblasts is also differentially affected by local cell density in microdomains of the gel surface. In this article we discuss the possible biochemical and behavioural mechanisms that may contribute to the different migratory phenotypes expressed by foetal, adult and transformed fibroblasts; these include: (1) cell-induced alterations in the orientation and or packing density of collagen fibres in the gel; (2) deposition of specific matrix macromolecules by the fibroblasts; (3) social interactions between the cells; and (4) secretion of soluble factors affecting cell ...
Fibroblasts are a main player in the tumor-inhibitory microenvironment. Upon tumor initiation and progression, fibroblasts can lose their tumor-inhibitory capacity and promote tumor growth. The molecular mechanisms that underlie this switch have not been defined completely. Previously, we identified four proteins overexpressed in cancer-associated fibroblasts and linked to Rho GTPase ... read more signaling. Here, we show that knocking out the Ras homolog family member A (RhoA) gene in normal fibroblasts decreased their tumor-inhibitory capacity, as judged by neighbor suppression in vitro and accompanied by promotion of tumor growth in vivo. This also induced PC3 cancer cell motility and increased colony size in 2D cultures. RhoA knockout in fibroblasts induced vimentin intermediate filament reorganization, accompanied by reduced contractile force and increased stiffness of cells. There was also loss of wide F-actin stress fibers and large focal adhesions. In addition, we observed a significant ...
Background: Cardiac fibrosis is associated with a variety of heart diseases including atrial fibrillation (AF). Cardiac fibroblast is the major cell type in cardiac fibrogenesis cascade. However, the biological as well as electrophysiological properties of cardiac fibroblasts are not fully understood. In this study, we investigated the functional expression of voltage-gated and non-voltage-gated ion channels in artial fibroblasts from AF patients and SR patients, and their contribution to atrial fibrogenesis.. Methods: With informed consent, right atrial biopsies were obtained from AF patients or sinus rhythm (SR) patients undergoing cardiac surgery. Fibroblasts were dissociated from the biopsy samples from SR patients or AF patients. The freshly isolated cells were used for patch-clamp and ratio Ca2+-imaging experiments.. Results: We found that there are two types of voltage-gated outward potassium channels in the fibroblasts from SR patients: one is transient outward potassium current (Ito), ...
Cell lines. EBV-transformed LCLs and primary dermal fibroblasts were established as previously described (57). Previously used LCLs from an unaffected individual, AG1010 (24), termed control LCLs, were used in all experiments. In experiments with primary dermal fibroblasts, a previously used cell line from a healthy individual, 82-6 (30), termed control fibroblasts 1, was used for all experiments. To corroborate the findings in some experiments, additional cell lines from unaffected individuals (IMR-90, NHDF, and 1101-SK) were used and are termed control fibroblasts 2, 3, and 4, respectively. 88-1 normal fibroblasts were also used. Primary dermal fibroblasts were maintained in DMEM supplemented with 10% FBS. Human osteosarcoma (U2OS) and non-small-cell lung cancer (H1299) cell lines were also maintained in DMEM supplemented with 10% FBS. LCLs were maintained in RPMI medium supplemented with 10% FBS. Cell lines were obtained from Junko Oshima, Lyubomir Vassilev (Serono Research and Development ...
COPD is associated with disturbed tissue repair, possibly due to TGF-β-regulated miRNA changes in fibroblasts. Our aim was to identify TGF-β-regulated miRNAs and their differential regulation and expression in COPD compared to control fibroblasts. Small RNA sequencing was performed on TGF-β-stimulated and unstimulated lung fibroblasts from 15 COPD patients and 15 controls. Linear regression was used to identify TGF-β-regulated and COPD-associated miRNAs. Interaction analysis was performed to compare miRNAs that responded differently to TGF-β in COPD and control. Re-analysis of previously generated Ago2-IP data and Enrichr were used to identify presence and function of potential target genes in the miRNA-targetome of lung fibroblasts. In total, 46 TGF-β-regulated miRNAs were identified in COPD and 86 in control fibroblasts (FDR , 0.05). MiR-27a-5p was the most significantly upregulated miRNA. MiR-148b-3p, miR-589-5p and miR-376b-3p responded differently to TGF-β in COPD compared to control ...
AppliedStemCell eCommerce Platform Human Skin Cells (Dermal Fibroblasts) (DMD) [ASE-5014] - Catalog Number ASE-5014 Quantity 5.0 x 105 cells/mL Product Information Description Human fibroblasts are derived from cultured skin explants. These fibroblasts ar
AppliedStemCell eCommerce Platform Human Skin Cells (Dermal Fibroblasts) (LCP) [ASE-5055] - Catalog Number ASE-5055 Quantity 5.0 x 105 cells/mL Product Information Description Human fibroblasts are derived from cultured skin explants. These fibroblasts ar
Fibrotic diseases are characterized by the accumulation of extracellular matrix together with distortion and disruption of tissue architecture. Phosphodiesterase (PDE)4 inhibitors, by preventing the breakdown of cAMP, can inhibit fibroblast functions and may be able to mitigate tissue remodeling. Transforming growth factor (TGF)-β1, a mediator of fibrosis, can potentially modulate cAMP by altering PGE2 metabolism. The present study assessed whether PDE4 inhibitors functionally antagonize the profibrotic activity of fibroblasts stimulated by TGF-β1. The PDE4 inhibitors roflumilast and rolipram both inhibited fibroblast-mediated contraction of three-dimensional collagen gels and fibroblast chemotaxis toward fibronectin in the widely studied human fetal lung fibroblast strain HFL-1 and several strains of fibroblasts from adult human lung. Roflumilast was ~10-fold more potent than rolipram. There was a trend for PDE4 inhibitors to inhibit more in the presence of TGF-β1 (0.05 , P , 0.08). The ...
During the last decade it has become clear that periodontal ligament fibroblasts may contribute to the in vitro differentiation of osteoclasts. We surveyed the current findings regarding their osteoclastogenesis potential. Periodontal ligament fibroblasts have the capacity to select and attract osteoclast precursors and subsequently to retract and enable migration of osteoclast precursors to the bone surface. There, fusion of precursors takes place, giving rise to osteoclasts. The RANKL-RANK-osteoprotegerin (OPG) axis is considered crucial in this process. Periodontal ligament fibroblasts produce primarily OPG, an osteoclastogenesis-inhibitory molecule. However, they may be influenced in vivo by direct or indirect interactions with bacteria or by mechanical loading. Incubation of periodontal ligament fibroblasts with bacteria or bacterial components causes an increased expression of RANKL and other osteoclastogenesis-stimulating molecules, such as tumor necrosis factor-α and macrophage-colony ...
TY - JOUR. T1 - The effect of carboxymethyl-chitosan on proliferation and collagen secretion of normal and keloid skin fibroblasts. AU - Chen, Xi Guang. AU - Wang, Zhen. AU - Liu, Wan Shun. AU - Park, Hyun Jin. PY - 2002/12. Y1 - 2002/12. N2 - In this study, different molecular weight CM-chitosans were prepared and the effects on the growth and collagen secretion of normal skin fibroblasts and keloid fibroblasts were investigated in vitro. CM-chitosan promoted the proliferation of the normal skin fibroblast significantly but inhibited the proliferation of keloid fibroblast. The higher CM-chitosan concentration had a higher initial effect and the lower CM-chitosan concentration had a longer affecting time to the normal skin fibroblast. The lower molecular weight CM-chitosan had significant twofold activities. The CM-chitosan could reduce the ratio of type I/III collagen in keloid fibroblast by inhibiting the secretion of collagen type I; and had no effect on the secretion of types I and III ...
Normal human fibroblasts display a limited lifespan in culture, which is due to a steadily decreasing fraction of cells that are able to proliferate. Using antibodies that react with antigens present in proliferating cells only, in an indirect immunofluorescence assay, we have estimated the fraction of proliferating cells in cultures of normal human fibroblasts. Furthermore, we have estimated the rate of decline in the fraction of proliferating cells during the process of cellular ageing by application of the assay to normal human fibroblasts throughout their lifespan in culture. Werners Syndrome is an autosomal recessive disease in which individuals display symptoms of ageing prematurely. Werners Syndrome fibroblasts display a reduced lifespan in culture compared with normal human fibroblasts. Like normal human fibroblasts, the growth of Werners Syndrome fibroblasts is characterised by a decreasing fraction of cells reacting with the proliferation-associated antibodies throughout their ...
Keloid pathogenesis occurs due to the longer duration of inflammation and the increase in the production of several factors such as TGF-β1 that causes the increase of fibroblast proliferation and collagen synthesis. The role of B4 Leukotriene (LTB4) in keloid pathogenesis particularly in the inflammation phase and tissue proliferation has not been clearly elucidated. The present study was to analyze the levels of LTB4, TGF-β1 and collagen in keloid fibroblast and normal skin fibroblast. Fibroblasts were cultured by applying explant method to the keloid and normal skin of the petient with the keloid. The measurement of the levels of LTB4, TGF-β1 and collagen was conducted by using Elisa method and triplicate was conducted subsequently. Statistic testing was performed through unpaired t test. The experiment was carried out in cell culture laboratory of The Faculty of Medicine Padjajaran University Bandung. The levels of LTB4, TGF-β1 are higher in keloid fibroblast, despite the fact that it does not
Proteoglycan from salmon nasal cartilage promotes in vitro wound healing of fibroblast monolayers via the CD44 receptorProteoglycan from salmon nasal cartilage promotes in vitro wound healing of fibroblast monolayers via the CD44 receptor ...
Enalaprilat (Ena.), an angiotensin II (Ang II) converting enzyme inhibitor (ACEI), can produce some therapeutic effects on hypertension, ventricular hypertrophy and myocardial remodeling in clinic, but its precise mechanism, especially its signaling pathways remain elusive. In this study, cardiac fibroblasts (CFb) was isolated by the trypsin digestion method; a BrdU proliferation assay was adopted to determine cell proliferation; an immunofluorescence assay was used to measure intracellular reactive oxygen species (ROS); immunocytochemistry staining and Western blotting assay were used to detect phosphorylated p38 mitogen activated protein kinase (p-p38MAPK) and transforming growth factor-β1 (TGF-β1) protein expression, respectively. The results showed that Ang II (10-7 M) stimulated the cardiac fibroblast proliferation which was inhibited by NAC (an antioxidant), SB203580 (a p38MAPK inhibitor) or enalaprilat; Ang II caused an burst of intracellular ROS level within thirty minutes, an increase in p
Over the last years, electronic cigarettes (ECs) have become more popular, particularly in individuals who want to give up smoking tobacco. The aim of the present study was to assess the influence of the different e-smoking liquids on the viability and proliferation of human periodontal ligament fibroblasts. For this study six test solutions with components from ECs were selected: lime-, hazelnut- and menthol-flavored liquids, nicotine, propylene glycol, and PBS as control group. The fibroblasts were incubated up to 96 h with the different liquids, and cell viability was measured by using the PrestoBlue® reagent, the ATP detection and the migration assay. Fluorescence staining was carried out to visualize cell growth and morphology. Data were statistically analyzed by two-tailed one-way ANOVA. The cell viability assay showed that the proliferation rates of the cells incubated with nicotine or the various flavored liquids of the e-cigarettes were reduced in comparison to the controls, though not all
OBJECTIVE To compare the mechanotransduction caused by cyclic and static mechanical strains in human periodontal ligament fibroblasts (hPDLFs) cultured under identical conditions. MATERIALS AND METHODS hPDLFs, originating from the same donors, were exposed either to cyclic or to static tensile strain using specially designed devices and under identical culture conditions. Activation of all members of mitogen-activated protein kinases (MAPKs) was monitored by western immunoblot analysis. Expression levels of immediate/early genes c-fos and c-jun were assessed with quantitative real-time polymerase chain reaction. RESULTS Time course experiments revealed that both types of stresses activate the three members of MAPK, that is ERK, p38, and JNK, with cyclic stress exhibiting a slightly more extended activation. Further downstream, both stresses upregulate the immediate/early genes c-fos and c-jun, encoding components of the activator protein-1 (AP-1), a key transcription factor in osteoblastic ...
TY - JOUR. T1 - Differential binding of 125I-IGF-I preparations to human fibroblast monolayers. AU - Conover, C. A.. AU - Misra, P.. AU - Hintz, R. L.. AU - Rosenfeld, R. G.. N1 - Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 1988. Y1 - 1988. N2 - Specific, high affinity binding of 125I-IGF-I to the type I IGF receptor on human fibroblast monolayers was not altered by varying feeding schedules, serum lots, washing procedures, or incubation times and temperatures. However, markedly different competitive binding curves were obtained when different iodinated IGF-I preparations were used. Five of six radioligands bound preferentially to the type I IGF receptor on human fibroblast monolayers, with 50% displacement at 4-8 μg/l unlabelled IGF-I; with one radioligand a paradoxical 20-200% increase in 125I-IGF-I binding was observed at low concentrations of unlabelled IGF-I, while concentrations as high as 100 μg/l IGF-I failed to displace this radioligand. The latter binding ...
TY - JOUR. T1 - Lysocardiolipin acyltransferase regulates TGF-β mediated lung fibroblast differentiation. AU - Huang, Long Shuang. AU - Jiang, Peiyue. AU - Feghali-Bostwick, Carol. AU - Reddy, Sekhar P.. AU - Garcia, Joe G.N.. AU - Natarajan, Viswanathan. PY - 2017/11. Y1 - 2017/11. N2 - Lysocardiolipin acyltransferase (LYCAT), a cardiolipin remodeling enzyme, plays a key role in mitochondrial function and vascular development. We previously reported that reduced LYCAT mRNA levels in peripheral blood mononuclear cells correlated with poor pulmonary function outcomes and decreased survival in IPF patients. Further LYCAT overexpression reduced lung fibrosis, and LYCAT knockdown accentuated experimental pulmonary fibrosis. NADPH Oxidase 4 (NOX4) expression and oxidative stress are known to contribute to lung fibroblast differentiation and progression of fibrosis. In this study, we investigated the role of LYCAT in TGF-β mediated differentiation of human lung fibroblasts to myofibroblasts, and ...
TY - JOUR. T1 - Effects of (1→3), (1→6)-β-D-glucan behavior in human dermal fibroblast cells under serum starvation. AU - Woo, Yeon I.. AU - Son, Hyun Joo. AU - Lim, Hye Ryeon. AU - Lee, Mi Hee. AU - Baek, Hyun Sook. AU - Tsubaki, Kazufumi. AU - Park, Jong Chul. PY - 2007. Y1 - 2007. N2 - Glucans have been reported to stimulate immunity and to promote wound healing. Adult human dermal fibroblast (aHDF) cultured in serum free (serum-starvation). Proliferation of aHDF was measured at various concentrations of β-glucan by MTT assay, and migration was observed for 36h on microscope. The result of fibroblast bioassay, β-glucan had positive influence. In this study, the direct effects of β-glucan on proliferation and migration of human dermal fibroblasts were examined in vitro. That means β-D-glucan has the effect to enhance proliferation and aHDF migration speed, and has the potential as a wound healing agent.. AB - Glucans have been reported to stimulate immunity and to promote wound ...
The roles of MEK, ERK, the epsilon and alpha isoforms of protein kinase C (PKC), and caveolin-1 in regulating collagen expression were studied in normal lung fibroblasts. Knocking down caveolin-1 gave particularly striking results. A 70% decrease caused a 5-fold increase in MEK/ERK activation and collagen expression. The combined data reveal a branched signaling pathway. In its central portion MEK activates ERK, leading to increased collagen expression. Two branches converge on MEK/ERK. In one, increased PKCepsilon leads to MEK/ERK activation. In another, increased PKCalpha induces caveolin-1 expression, which in turn inhibits MEK/ERK activation and collagen expression. Lung fibroblasts from scleroderma patients with pulmonary fibrosis showed altered signaling. Consistent with their overexpression of collagen, scleroderma lung fibroblasts contain more activated MEK/ERK and less caveolin-1 than normal lung fibroblasts. Because cutaneous fibrosis is the hallmark of scleroderma, we also studied ...
Chronic kidney disease (CKD) is a leading cause of end stage renal disease (ESRD) and cardiovascular morbidity and mortality worldwide, resulting in a growing social and economic burden. The prevalence and burden of CKD is anticipated to further increase over the next decades as a result of aging. In the pathogenesis of CKD, irrespective of the etiology, resident fibroblasts are key players and have been demonstrated to play crucial roles for disease initiation and progression. In response to injury, resident fibroblasts transdifferentiate into myofibroblasts that express alpha smooth muscle actin (αSMA) and have an increased capacity to produce large amounts of extracellular matrix (ECM) proteins, leading to renal fibrosis. In addition to this fundamental role of fibroblasts as drivers for renal fibrosis, growing amounts of evidence have shown that resident fibroblasts are also actively involved in initiating and promoting inflammation during kidney injury. During the myofibroblastic transition
Nature Reviews Nephrology. fibroblast synovial cells and chondrocytes [13]. Obese osteoarthritis patients exhibit an inflammatory synovial fibroblast phenotype, which is regulated by the long non coding RNA MALAT1 November 2019 Arthritis and Rheumatology 72(4) Because we focused on the synovium, mTOR and lysophosphatidic acid were not described in greater detail, as these factors were only found to be elevated in chondrocytes/cartilage and not in synovial fibroblasts or the synovium. eCollection 2019. Bank RA Verzijl N Lafeber FP Tekoppele JM. In the past, OA was considered a disease of the cartilage only. The Smad-independent TAK-1 pathway has been shown to have profibrotic effects in regulating the expression of ECM proteins, including collagens and fibronectin [41]. OBJECTIVE: Changes in rheumatoid arthritis synovial fibroblast (RASF) gene expression are usually defined by a comparison to osteoarthritis synovial fibroblasts (OASFs). CTGF, like TGF-β, is found to be elevated in many fibrotic ...
Authors: Tang CH, Hsu CJ, Yang WH, Fong YC. Patients with rheumatoid arthritis (RA) are at increased risk of developing infections and appear to be particularly susceptible to septic arthritis. Lipoteichoic acid (LTA), a cell wall component of Gram-positive bacteria is an amphiphilic, negatively charged glycolipid. However, the effects of LTA on human synovial fibroblasts are largely unknown. We investigated the signaling pathway involved in IL-6 production stimulated by LTA in rheumatoid arthritis synovial fibroblasts (RASF). LTA caused concentration- and time-dependent increases in IL-6 production. LTA-mediated IL-6 production was attenuated by Toll-like receptor 2 (TLR2) monoclonal antibody or siRNA. Pretreatment with PKCdelta inhibitor (rottlerin), c-Src inhibitor (PP2), AP-1 inhibitor (tanshinone IIA) and NF-kappaB inhibitor (PDTC and TPCK) also inhibited the potentiating action of LTA. However, focal adhesion kinase (FAK) mutant and siRNA did not affect LTA-mediated IL-6 production. ...
TY - JOUR. T1 - Modulation of fibroblast morphology and adhesion during collagen matrix remodeling. AU - Tamariz, Elisa. AU - Grinnell, Frederick. N1 - Copyright: Copyright 2005 Elsevier B.V., All rights reserved.. PY - 2002/11/1. Y1 - 2002/11/1. N2 - When fibroblasts are placed within a three-dimensional collagen matrix, cell locomotion results in translocation of the flexible collagen fibrils of the matrix, a remodeling process that has been implicated in matrix morphogenesis during development and wound repair. In the current experiments, we studied formation and maturation of cell-matrix interactions under conditions in which we could distinguish local from global matrix remodeling. Local remodeling was measured by the movement of collagen-embedded beads towards the cells. Global remodeling was measured by matrix contraction. Our observations show that no direct relationship occurs between protrusion and retraction of cell extensions and collagen matrix remodeling. As fibroblasts globally ...
TY - JOUR. T1 - WISP1, a pro-mitogenic, pro-survival factor, mediates tumor necrosis factor-α (TNF-α)-stimulated cardiac fibroblast proliferation but inhibits TNF-α-induced cardiomyocyte death. AU - Venkatachalam, Kaliyamurthi. AU - Venkatesan, Balachander. AU - Valente, Anthony J.. AU - Melby, Peter. AU - Nandish, Sailesh. AU - Reusch, Jane E B. AU - Clark, Robert A.. AU - Chandrasekar, Bysani. PY - 2009/5/22. Y1 - 2009/5/22. N2 - WNT1-inducible signaling pathway protein-1 (WISP1), a member of the CYR61/CTGF/Nov family of growth factors, can mediate cell growth, transformation, and survival. Previously we demonstrated that WISP1 is up-regulated in post-infarct heart, stimulates cardiac fibroblast proliferation, and is induced by the proinflammatory cytokine tumor necrosis factor-α (TNF-α). Here we investigated (i) the localization of TNF-α and WISP1 in post-infarct heart, (ii) the mechanism of TNF-α-mediated WISP1 induction in primary human cardiac fibroblasts (CF), (iii) the role of ...
Leukoregulin (LR), a T-cell-derived growth factor, modulates fibroblast functions in vitro [Mauviel, Rédini, Hartmann, Loyau & Pujol (1991) J. Cell Biol. 113, 1455-1462]. In the present study, incubation of human dermal fibroblasts with LR (0.1-2 units/ml) resulted in decreases in the mRNA steady-state levels for alpha 1(I), alpha 2(I) and alpha 1(III), but not alpha 2(V), collagen genes. LR also down-regulated alpha 2(I) collagen promoter activity in transient cell transfections of control cells as well as those incubated with transforming growth factor-beta, a potent up-regulator of collagen type I gene expression. Thus LR is a strong inhibitor of type I collagen gene expression, acting at the level of transcription. ...
Huang, S.-M., Zuo, X., Li, J. J., Li, S. F. Y., Bay, B. H. and Ong, C. N. (2012), Metabolomics Studies Show Dose-Dependent Toxicity Induced by SiO2 Nanoparticles in MRC-5 Human Fetal Lung Fibroblasts. Advanced Healthcare Materials, 1: 779-784. doi: 10.1002/adhm.201200114 ...
Cynomolgus Monkey Primary Cardiac Fibroblasts. Catalog No. MK-6049. Suggested Medium: Catalog No. M2267 Fibroblast Medium /w Kit (500 ml). Product Description. Monkey Primary Cardiac Fibroblasts from Cell Biologics are isolated from tissue of Cynomolgus Monkey. Monkey Primary Cardiac Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml and is delivered frozen. Monkey Primary Cardiac Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.. Laboratory Applications. Standard biochemical procedures performed ...
TY - JOUR. T1 - Clinical variation in X-linked adrenoleukodystrophy. T2 - Fatty acid and lipid metabolism in cultured fibroblasts. AU - Boles, Debra J.. AU - Craft, Debra A.. AU - Padgett, David A.. AU - Loria, Roger M.. AU - Rizzo, William B.. PY - 1991/2. Y1 - 1991/2. N2 - To determine whether the clinical phenotype of ALD correlates with the extent of metabolic abnormality, we investigated VLFA metabolism in cultured fibroblasts from patients with the clinically severe childhood form of ALD and the milder AMN variant. No differences were seen in the content of neutral lipids or phospholipids, in incorporation of [1-14C]lignocerate into cellular lipids, or in the fatty acid composition of fibroblasts from patients with childhood ALD or AMN. [1-14C]Lignocerate oxidation was deficient to a similar extent (35-40% of normal) in both intact fibroblasts and cell homogenates from patients with childhood ALD and AMN. With the use of fibroblast homogenates, oxidation of lignocerate was partially ...
Chronic airway diseases like COPD and asthma are usually accompanied with airway fibrosis. Myofibroblasts, which are characterized by expression of smooth muscle actin (alpha-SMA), play an important role in a variety of developmental and pathological processes, including fibrosis and wound healing. Sphingosylphosphorylcholine (SPC), a sphingolipid metabolite, has been implicated in many physiological and pathological conditions. The current study tested the hypothesis that SPC may modulate tissue remodeling by affecting the expression of a-SMA in human fetal lung fibroblast (HFL-1) and fibroblast mediated gel contraction. The results show that SPC stimulates a-SMA expression in HFL-1 and augments HFL-1 mediated collagen gel contraction in a time- and concentration-dependent manner. The a-SMA protein expression and fibroblast gel contraction induced by SPC was not blocked by TGF-beta 1 neutralizing antibody: However, it was significantly blocked by S1P2 receptor antagonist JTE-013, the ...
Several cell surface proteins (Mr=120,000, 90,000, 63,000 and 47,000) apparently integral to embryonic fibroblast plasma membranes were extracted with detergent and isolated by collagen affinity chromatography. Certain of these proteins (Mr=120,000, 90,000 and 47,000) were specifically eluted from collagen affinity columns by synthetic peptides containing the amino acid sequence arginyl-glycyl-aspartic acid (RGD). These data show that a number of collagen binding proteins exist on the embryonic fibroblast cell surface. Some of the proteins may be collagen receptors binding to RGD sequences in the collagen molecule while at least one of the proteins (Mr=63,000) recognizes features other than RGD. ...
Synovial fibroblasts isolated from human synovium. High quality synovial fibroblasts from human synovium cryopreserved and provided as frozen aliquots. Human synovial fibroblasts can be from patients with osteoarthritis, rheumatoid arthritis, or with no
Synovial fibroblasts isolated from human synovium. High quality synovial fibroblasts from human synovium cryopreserved and provided as frozen aliquots. Human synovial fibroblasts can be from patients with osteoarthritis, rheumatoid arthritis, or with no
TY - CONF. T1 - Effects of THz radiation on human fibroblasts in-vitro: Exposure set-up and biological endpoints. AU - Gallerano, G.P.. AU - Giovenale, E.. AU - Nenzi, P.. AU - De Amicis, A.. AU - De Sanctis, S.. AU - Di Cristofaro, S.. AU - Franchini, V.. AU - Lista, F.. AU - Regalbuto, E.. AU - Sgura, A.. AU - Coluzzi, E.. AU - Bei, R.. AU - Fantini, M.. AU - Benvenuto, M.. AU - Masuelli, L.. PY - 2014/11/13. Y1 - 2014/11/13. N2 - In vitro exposures of human fibroblasts have been performed in a wide band between 100 and 150 GHz using the ENEA Compact Free Electron Laser. The methodology of the study and the exposure set-up will be presented together with an analysis of preliminary results.. AB - In vitro exposures of human fibroblasts have been performed in a wide band between 100 and 150 GHz using the ENEA Compact Free Electron Laser. The methodology of the study and the exposure set-up will be presented together with an analysis of preliminary results.. UR - ...
Senussi O.A.; Carwright T.; Thompson P., 1979: Resolution of human fibroblast interferon into 2 distinct classes by thiol exchange chromatography
Synthesis of new tissue by fibroblasts is required for tissue rebuilding in response to injury. Fibroblast migration from surrounding healthy tissue into the fibrin-fibronectin provisional matrix deposited upon injury is a key rate-limiting step of this stage of tissue repair. These events must be tightly regulated. Excessive deposition of scar tissue is the major hallmark of fibrotic disease. Tenascin-C is an extracellular matrix glycoprotein that is transiently expressed upon tissue injury, where it is specifically localized to the wound edge, and persistently up-regulated in fibrotic disease. We have shown that full-length tenascin-C promotes fibroblast migration within fibrin-fibronectin matrices and we have mapped the domains within the molecule critical for enhancing migration. We also demonstrated that specific fragments of tenascin-C inhibit fibroblast migration. These results suggest that transient expression of tenascin-C at the wound boundary is key to tissue repair: its induction recruits
The understanding of cell-surface interactions plays an important role for the biomaterials development and bioengineering. Although it is already known that amine groups increase the cell adhesion and proliferation, the influence of amine layers properties on cell viability is the subject of further investigation. In this work, amine-rich coatings were prepared by low pressure plasma polymerization of cyclopropylamine using radio frequency (RF) capacitively coupled discharge. Normal human dermal fibroblasts were chosen for the monitoring of biological response to the properties of amine layers. As a superior technique for the label-free monitoring of the cell-surface interaction, coherence-controlled holographic microscopy (CCHM) was exploited. CCHM enables quantitative phase imaging. From such images, valuable morphological parameters of cells directly related to the cell dry mass can be extracted. Based on those parameters, viability of cells cultivated on the plasmatreated surfaces with ...
TY - JOUR. T1 - Marked reduction of alcohol dehydrogenase in keratoconus corneal fibroblasts. AU - Mootha, Venkateswara. AU - Kanoff, J. M.. AU - Shankardas, J.. AU - Dimitrijevich, S.. PY - 2009/4/10. Y1 - 2009/4/10. N2 - Purpose: To identify differentially expressed genes in keratoconus (KC) corneal fibroblasts. Methods: Stromal keratocytes (having a fibroblast morphology) from KC keratoplasty specimens and eye bank donor corneas were isolated and expanded using a serum containing medium. RNA was isolated from three KC fibroblast cultures and five eye bank donor cornea fibroblast cultures. The targets from the cultured fibroblasts were hybridized to the Affymetrix U133 Plus 2.0 microarrays. Western blot analyses of cell lysates were performed to examine protein levels of interest in the two groups. Protein levels of select differentially expressed genes were further examined by immunohistochemistry. Keratocyte staining of archived KC keratoplasty specimens were graded using a 0 to 3+ scale and ...
The addition of cardiac fibroblast-conditioned medium to purified cardiomyocyte cultures was associated with 1) cell hypertrophy, 2) vimentin expression in the presence of MyHC, and 3) markedly reduced spontaneous rhythmic contractions intrinsic to untreated cultured cardiomyocytes. These effects on cardiomyocyte structure and chronotropy in fibroblast-conditioned medium differed both in nature and time frame from the dedifferentiation or fetal gene program previously delineated in cardiomyocytes cultured under standard conditions (9, 11-14). For example, the progressive emergence of α-smooth muscle actin across all culture conditions was consistent with the dedifferentiation paradigm in contrast to vimentin expression specific to the fibroblast-conditioned medium (9, 14). These data suggest that cardiac fibroblasts introduced a soluble factor or factors into the medium that altered cardiomyocyte function and phenotype by either 1) a direct cell membrane or intracellular interaction or 2) ...
ATCC® Normal Adult Human Primary Dermal Fibroblasts, when grown in Fibroblast Basal Media supplemented with Fibroblast Growth Kit components, provide an ideal cell system to propagate dermal fibroblasts in either serum-free or low serum conditions. The cells are cryopreserved in their first passage to ensure the highest viability and plating efficiency. ATCC® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Endogenous electric currents generated instantly at skin wounds direct migration of epithelial cells and are likely to be important in wound healing. Migration of fibroblasts is critical in wound healing. It remains unclear how wound electric fields guide migration of dermal fibroblasts. We report here that mouse skin wounds generated endogenous electric currents for many hours. Human dermal fibroblasts of both primary and cell-line cultures migrated directionally but slowly toward the anode in an electric field of 50-100 mV mm−1. This is different from keratinocytes, which migrate quickly to the cathode. It took more than 1 hour for dermal fibroblasts to manifest detectable directional migration. Larger field strength (400 mV mm−1) was required to induce directional migration within 1 hour after onset of the field. Phosphatidylinositol-3-OH kinase (PI3 kinase) mediates cathode-directed migration of keratinocytes. We tested the role of PI3 kinase in anode-directed migration of fibroblasts. ...
Cells invade tissues by sending out actin-rich protrusions called invadopodia that contain proteolytic enzymes that degrade the surrounding extracellular matrix (ECM). Fibroblasts without Snail1 formed fewer invadopodia and were less able to degrade the ECM. Rowe et al. transplanted the Snail1-deficient fibroblasts into chick embryos and found that they were completely unable to penetrate the basement membrane and the complex mix of ECM proteins beneath. Moreover, unlike wild-type fibroblasts, Snail1-deficient cells didnt stimulate the ingrowth of new blood vessels-another key function of fibroblasts during wound healing and tissue remodeling.. The team thinks that in addition to its role in EMT, Snail1 also acts as a master regulator of fibroblast function. In cancer cells, says author Grant Rowe, sustained Snail1 expression may not only cause a loss of epithelial markers but also promote tumor aggression by stimulating tissue invasion and angiogenesis.. ...
TY - JOUR. T1 - Heat Shock Protein 90 Inhibitor (17-AAG) Induces Apoptosis and Decreases Cell Migration/Motility of Keloid Fibroblasts. AU - Yun, In Sik. AU - Lee, Mi Hee. AU - Rah, Dong Kyun. AU - Lew, Dae Hyun. AU - Park, Jong Chul. AU - Lee, Won Jai. PY - 2015/7/4. Y1 - 2015/7/4. N2 - Background: The regulation of apoptosis, proliferation, and migration of fibroblasts is altered in keloids. The 90-kDa heat shock protein (heat shock protein 90) is known to play a key role in such regulation. Therefore, the authors investigated whether the inhibition of heat shock protein 90 in keloid fibroblasts could induce apoptosis and attenuate keloid fibroblast proliferation and migration. Methods: The authors evaluated heat shock protein 90 expression in keloid tissues with immunohistochemistry. The authors used cell viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays and annexin V/propidium iodide staining for apoptosis, a wound healing model and cell tracking system to ...
TY - JOUR. T1 - MicroRNA expression analysis of human skin fibroblasts treated with high-fluence light-emitting diode-red light. AU - Mamalis, Andrew. AU - Koo, Eugene. AU - Tepper, Clifford G. AU - Jagdeo, Jared. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Skin fibrosis is a chronic debilitating feature of several skin diseases that lead to characteristic increases in dermal fibroblast proliferation and collagen deposition through upregulation in components of the transforming growth factor beta (TGF-B)/SMAD pathway. In contrast to ultraviolet phototherapy, high-fluence light-emitting diode-generated red light (HF-LED-RL, 633 ± 15 nm) is a safe, economic and non-invasive therapy with in vitro evidence that supports modulation of the key cellular characteristics involved in the pathogenesis of skin fibrosis. Limited data exists pertaining to the effects of HF-LED-RL on human skin fibroblast microRNA (miRNA). Herein, we explored the effects of HF-LED-RL on fibroblast miRNA levels using RNA-seq and miRNA ...
Endothelial progenitor cell (EPC) transplantation is a promising therapy for ischemic diseases such as ischemic myocardial infarction and hindlimb ischemia. However, limitation of EPC sources remains a major obstacle. Direct reprogramming has become a powerful tool to produce EPCs from fibroblasts. Some recent efforts successfully directly reprogrammed human fibroblasts into functional EPCs; however, the procedure efficacy was low. This study therefore aimed to improve the efficacy of direct reprogramming of human fibroblasts to functional EPCs. Human fibroblasts isolated from foreskin were directly reprogrammed into EPCs by viral ETV2 transduction. Reprogramming efficacy was improved by culturing transduced fibroblasts in hypoxia conditions (5 % oxygen). Phenotype analyses confirmed that single-factor ETV2 transduction successfully reprogrammed dermal fibroblasts into functional EPCs. Hypoxia treatment during the reprogramming procedure increased the efficacy of reprogramming from 1.21 ± 0.61 % in
TY - JOUR. T1 - Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation. AU - Malecka, Anna. AU - Wang, Qunwei. AU - Shah, Sabaria. AU - Sutavani, Ruhcha V.. AU - Spendlove, Ian. AU - Ramage, Judith M.. AU - Greensmith, Julie. AU - Franks, Hester A.. AU - Gough, Michael J.. AU - Saalbach, Anja. AU - Patel, Poulam M.. AU - Jackson, Andrew M.. PY - 2016/8/1. Y1 - 2016/8/1. N2 - Dendritic cell function is modulated by stromal cells, including fibroblasts. Although poorly understood, the signals delivered through this crosstalk substantially alter dendritic cell biology. This is well illustrated with release of TNF-α/IL-1β from activated dendritic cells, promoting PGE2 secretion from stromal fibroblasts. This instructs dendritic cells to up-regulate IL-23, a key Th17-polarizing cytokine. We previously showed that ionizing radiation inhibited IL-23 production by human dendritic cells in vitro. In the present study, we investigated the ...
Helena is a Postdoctoral Researcher in the lab, working to define fibroblast the role of fibroblast sub-types in human skin wound healing. She conducted her PhD thesis in the lab, funded by a Department Scholarship. This work led to a patent application, and the lab was subsequently awarded an Imperial Confidence in Concept grant, and a grant from The Rosetrees Trust, enabling Helena to continue on her thesis work as a postdoctoral researcher. There are multiple fibroblast populations found in adult skin dermis, three of which we are particularly interested in; papillary fibroblasts, reticular fibroblasts and dermal papilla fibroblasts. In 2013 it was shown that these arise from a common cellular progenitor during development, yet acquire distinct identities in adult skin [1]. Using cell culture, immunofluorescence, confocal microscopy, RT-PCR and next generation sequencing approaches, Helena is defining the morphological, molecular and behavioural differences between these three fibroblast ...
Interstitial flow emanates from tumors into the microenvironment where it promotes tumor cell invasion. Fibroblasts are key constituents of the tumor stroma that modulate the mechanical environment by matrix remodeling and contraction. Here, we explore how interstitial fluid flow affects fibroblast-tumor cell interactions. Using a 3-dimensional invasion assay and MDA-MB-435S cells cocultured with dermal fibroblasts in a collagen matrix, we showed a synergistic enhancement of tumor cell invasion by fibroblasts in the presence of interstitial flow. Interstitial flow also drove transforming growth factor (TGF)-β1 and collagenase-dependent fibroblast migration, consistent with previously described mechanisms in which flow promotes invasion through autologous chemotaxis and increased motility. Concurrently, migrating fibroblasts enhanced tumor cell invasion by matrix priming via Rho-mediated contraction. We propose a model in which interstitial flow promotes fibroblast migration through increased TGF-β1
Plasma Fibroblast therapy is an elective, aesthetic, beauty procedure that can be offered as an alternative to laser, injections, and surgical therapies to tighten, rejuvenate. and improve the skins appearance.. Plasma Fibroblast therapy targets fibroblast cells. Fibroblasts are collagen and protein-producing cells in the dermis layer of skin which is just below your outermost skin layer. Fibroblasts play an important role in maintaining skin firmness and tightness, as well as, helping skin wounds heal.. Plasma Fibroblast therapy uses a pen-like device that discharges a high-frequency energy charge or arc. Your procedure will be performed using the Plamere™ Premium Plasma Pen (FDA registered). The plasma pens tip does not directly touch the skin, but instead releases a targeted arc just above the skins surface. This arc creates a tiny, micro-injury in the skins surface layer due to a reaction called sublimation.. ...
TY - JOUR. T1 - Low-dose of ionizing radiation enhances cell proliferation via transient ERK1/2 and p38 activation in normal human lung fibroblasts. AU - Kim, Cha Soon. AU - Kim, Jin Mo. AU - Nam, Seon Young. AU - Yang, Kwang Hee. AU - Jeong, Meeseon. AU - Kim, Hee Sun. AU - Lim, Young Khi. AU - Kim, Chong Soon. AU - Jin, Young Woo. AU - Kim, Joon. PY - 2007/9/27. Y1 - 2007/9/27. N2 - This study shows the human cellular responses and the mechanism of low-dose ionizing radiation in CCD 18 Lu cells, which are derived from normal human lung fibroblasts. Cell proliferation and viability assay were measured for the cells following γ-irradiation using trypan blue, BrdU incorporation, and Wst-1 assay. We also examined genotoxicity using a micronuclei formation assay. The activation of the MAPKs pathway was determined by Western blot analysis, and the siRNA system was used to inhibit the expression of ERK1/2 and p38. We found that 0.05 Gy of ionizing radiation stimulated cell proliferation and did not ...
Allogeneic cells are the most attractive source for cell transplantation, as the use of xenogeneic cells is hampered by safety concerns and the use of autologous cells involves practical difficulties. The immune rejection of allogeneic cells can be overcome by physical immunoprotection provided by polymer encapsulation. To study the variability of cell and donor sources, we compared different primary human cells as candidates for gene therapy-mediated delivery of human erythropoietin (hEpo). DARC-3.1 fibroblasts, MDX-01 fibroblasts, and ARPE-19 retinal pigment epithelial cells were encapsulated into polyethersulfone hollow fibers and implanted for 1 month in nude mice as well as in immunocompetent and FK506-immunosuppressed mice to test their in vivo resistance, with the assumption that xenogeneic conditions constitute a stringent model for human application. DARC-3.1 fibroblasts showed the best survival, prompting us to evaluate cell lineages from the same donor (DARC-3.2) or another donor (DARC-4.3
TY - JOUR. T1 - Expression of hydrogen peroxide and glutathione metabolizing enzymes in human skin fibroblasts derived from donors of different ages. AU - Keogh, Bart P.. AU - Allen, R. G.. AU - Pignolo, Robert. AU - Horton, Joseph. AU - Tresini, Maria. AU - Cristofalo, Vincent J.. PY - 1996/6. Y1 - 1996/6. N2 - We have examined the activities and mRNA abundance of two hydrogen peroxide metabolizing enzymes (glutathione peroxidase and catalase), glutathione concen tration, and the activities of several enzymes that influence glutathione concentration, including glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G-6-PD), and γ-glutamylcysteine synthetase (γ-GCS), in 29 skin fibroblast lines derived from donors ranging in age from 14 gestational weeks to 94 years of age. H2O2 metabolizing enzyme activities and mRNA abundances were greater in skin fibroblast cultures established from postnatal donors than in fetally derived cultures. There were no significant differences in either of ...
2015 Guo et al. A key feature of lung fibrosis is the accumulation of myofibroblasts. Interleukin 13 (IL-13) is a pro-fibrotic mediator that directly and indirectly influences the activation of myofibroblasts. Transforming growth factor beta (TGF-β) promotes the differentiation of fibroblasts into myofibroblasts, and can be regulated by IL-13. However, IL-13s downstream signaling pathways are not completely understood.We previously reported that the transcription factor Yin Yang 1 (YY1) is upregulated in fibroblasts treated with TGF-β and in the lungs of mice and patients with pulmonary fibrosis. Moreover, YY1 directly regulates collagen and alpha smooth muscle actin (a-SMA) expression in fibroblasts. However, it is not known if IL-13 regulates fibroblast activation through YY1 expression. We hypothesize that IL-13 upregulates YY1 expression through regulation of AKT activation, leading to fibroblast activation. In this study we found that YY1 was upregulated by IL-13 in lung fibroblasts in a ...
Abstract: : Purpose: Adenovirus infection of the cornea manifests as punctate epithelial keratitis, geographic epithelial erosions, and delayed-onset subepithelial corneal infiltrates. We have previously shown that (1)adenovirus type 19 (Ad19) infection of human corneal fibroblasts (HCF) induces expression of the neutrophil chemokine interleukin-8 (IL-8); (2) Ad19 infection of HCF induces tyrosine phosphorylation of the intracellular signaling protein c-src; and (3) phosphorylation of c-src is necessary for the expression of IL-8 by Ad19-infected HCF. These data suggest that Ad19 induces IL-8 expression in HCF by a signaling cascade involving c-src. In the experiments described herein, we sought to determine whether adenovirus infection of HCF might induce other host responses dependent on c-src associated signaling pathways. Methods: HCF were derived from donor corneas and infected for one hour with Ad19, or mock-infected with virus-free media. Parallel experiments were performed with the ...
TY - JOUR. T1 - A cell cycle study of the effects of Con A on synchronized mouse embryo fibroblasts. T2 - Arrest and dissociation between uptake of thymidine and DNA synthesis. AU - Mallucci, L.. AU - Dunn, M.. AU - Wells, V.. AU - Delia, D.. PY - 1980. Y1 - 1980. N2 - We have examined the effects of 50 μg ml-1 of Con A added to synchronized mouse embryo fibroblasts at different times during the cell cycle. We found that Con A caused arrest of growth not solely by preventing G1-G0 cells from entering the S-phase but also by exerting a G2 block. We also found that Con A, which prevented commencement of S-phase, did not arrest cells already in S from reaching the G2 stage but inhibited the S-phase associated process of thymidine uptake. The inhibition was greater when the Con A receptors were extensively clustered.. AB - We have examined the effects of 50 μg ml-1 of Con A added to synchronized mouse embryo fibroblasts at different times during the cell cycle. We found that Con A caused arrest of ...
Among cells present in the tumor microenvironment, activated fibroblasts termed cancer-associated fibroblasts (CAFs), play a critical role in the complex process of tumor-stroma interaction. CAFs, one of the prominent stromal cell populations in most types of human carcinomas, have been involved in tumor growth, angiogenesis, cancer stemness, extracellular matrix remodeling, tissue invasion, metastasis and even chemoresistance. During the past decade, these activated tumor-associated fibroblasts have also been involved in the modulation of the anti-tumor immune response on various levels. In this review, we describe our current understanding of how CAFs accomplish this task as well as their potential therapeutic implications.
"Fibroblast". Genetics Home Reference. U.S. National Library of Medicine. 2014-05-05. Retrieved 2014-05-10. "Fibroblasts". ... It is thought that fibroblast stimulation by the thyroid stimulating hormone (TSH) receptor increases the deposition of ... The increased deposition of glycosaminoglycan is not fully understood, however, two mechanisms predominate: Fibroblast ...
It consists of fibroblasts harvested from the patient's own skin. It was approved for medical use in the United States in June ... "Autologous Fibroblasts". U.S. Food and Drug Administration (FDA). 29 June 2017. Archived from the original on 22 July 2017. ... July 2012). "A multicenter, double-blind, placebo-controlled trial of autologous fibroblast therapy for the treatment of ...
In the reactive stroma hypothesis, tumor cells cause the proliferation of fibroblasts and subsequent secretion of collagen. The ... Myofibroblastic cells in tumors are differentiated from fibroblasts for their positive staining of smooth-muscle actin (SMA). ... These scars are hypercellular with fibroblasts, myofibroblasts, and some immune cells present. The immature scars can be ... The tumor-induced stromal change hypothesis claims that tumor cells can dedifferentiate into fibroblasts and, themselves, ...
... mouth gingival fibroblasts; and eye (corneal endothelium and keratocytes, trabecular cells, ciliary epithelium, and ...
Monolayering' of fibroblasts. Exp. Cell Res. 6: 293-306. Abercrombie is also known for editing and co-founding the Penguin New ... I. Speed of movement of chick heart fibroblasts in relation to their mutual contacts. Exp. Cell Res. 5: 111-131. Abercrombie, M ...
... and basic fibroblast growth factor-induced osteoclast formation; bone taken from EP4(-/-) mice to re-absorb bone when induced ... and gingival fibroblasts. Standard prostanoids have the following relative efficacies in binding to and activating EP4: PGE2> ... mRNA through activation of p38 mitogen-activated protein kinase in interleukin-1 beta-treated human synovial fibroblasts". The ...
... a fibroblast cell line); rat PA adventitia fibroblasts; Baby hamster kidney cells; and diverse types of vascular endothelial ... "Hydroxyeicosatetraenoic acids released through the cytochrome P-450 pathway regulate 3T6 fibroblast growth". The Journal of ...
These appear similar to fibroblasts. The stroma also contains ordinary connective tissue such as reticular fibers and collagen ...
This is secreted by fibroblasts. The tunica externa provides basic structural support to blood vessels. It prevents vessels ...
It has been reported that the limited replicative capability of human fibroblasts observed in cell culture is far greater than ... Hayflick noticed that one of his cultures of embryonic human fibroblasts had developed an unusual appearance and that cell ... Carrel A, Ebeling AH (1921). "Age and multiplication of fibroblasts". J. Exp. Med. 34 (6): 599-606. doi:10.1084/jem.34.6.599. ... The experiment proceeded as follows: Hayflick mixed equal numbers of normal human male fibroblasts that had divided many times ...
ISBN 978-0-08-020575-5. Milo G E; Casto B C (1990). Transformation of human diploid fibroblasts. CRC Press. p. 104. ISBN 978-0- ...
Specifically preventing the formation of fibroblasts. An alternate irrigation with povidone-iodine may be used - if no contra- ...
Abercrombie, M; Heaysman, JE; Pegrum, SM (1970). "The locomotion of fibroblasts in culture. I. Movements of the leading edge". ... "Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts". Cell. 10 (3 ... is largely based on how cap formation occurs and the movement of carbon particles on the surfaces of migrating fibroblasts ...
Mouse Embryonic Fibroblasts (MEF) Gao, J; et al. (2010). "Characterization of OP9 as authentic mesenchymal stem cell line". J ...
Technique to culture fibroblasts in vitro]. Revue Française d'Études Cliniques et Biologiques. 5: 406-408. "Past ASHG Award ... In 2009, co-author Gautier claimed that the discovery was based on fibroblast tissue samples that she had prepared and noticed ...
It is expressed in lung fibroblasts. The protein is neddylated, which enhances the ubiquitination activity of SCF. Deneddylated ...
Fibroblasts are the most common cell type in connective tissue ECM, in which they synthesize, maintain, and provide a ... Wang JH, Thampatty BP, Lin JS, Im HJ (April 2007). "Mechanoregulation of gene expression in fibroblasts". Gene. 391 (1-2): 1-15 ... Elastins are synthesized by fibroblasts and smooth muscle cells. Elastins are highly insoluble, and tropoelastins are secreted ... Hadjipanayi E, Mudera V, Brown RA (February 2009). "Close dependence of fibroblast proliferation on collagen scaffold matrix ...
Fibroblasts, including periodontal fibroblasts, synthesize osteonectin. This protein is synthesized by macrophages at sites of ... It disrupts focal adhesions in fibroblasts. It also regulates the proliferation of some cells, especially endothelial cells, ... Osteonectin is expressed by a wide variety of cells, including chondrocytes, fibroblasts, platelets, endothelial cells, ... "An osteonectinlike protein in porcine periodontal ligament and its synthesis by periodontal ligament fibroblasts". Canadian ...
These include fibroblasts and endocrine cells. The symptoms of a sex-cord or stromal ovarian tumor can differ from other types ... and fibroblast growth factor 23. Use of blood test panels may help in diagnosis. The OVA1 panel includes CA-125, beta-2 ...
The fibroblasts within the granulation tissue develop into chondroblasts which also form hyaline cartilage. These two new ... Within this area, the fibroblasts replicate. Within 7-14 days, they form a loose aggregate of cells, interspersed with small ... Other sources of precursor cells are the bone marrow (when present), endosteum, small blood vessels, and fibroblasts. Primary ...
There is no increase in fibroblasts. Over time, secondary hyperkeratosis may occur, which may become verruciform. Many of these ... The serum contains circulating factors which stimulate fibroblasts to increase synthesis of glycosaminoglycans. There are ...
The FGF (Fibroblast Growth Factor) pathway stimulation leads to Smad4 phosphorylation by Erk of the canonical MAPK site located ... It is also produced by fibroblasts. The functional SMAD 4 participates in the regulation of the TGF-β signal transduction ... "TGF-beta1 stimulates human AT1 receptor expression in lung fibroblasts by cross talk between the Smad, p38 MAPK, JNK, and PI3K ...
The perineurium is composed from fibroblasts. In the peripheral nervous system, the myelin sheath of each axon in a nerve is ...
Carcinoma associated fibroblasts (CAFs) are a heterogenous group of fibroblasts whose function is pirated by cancer cells and ... fibroblast specific protein 1 (FSP-1) and fibroblast activation protein (FAP). None of these factors can be used to ... As fibroblasts, CAFs are able to rework the ECM to include more paracrine survival signals such as IGF-1 and IGF-2, thus ... As many fibroblasts are transformed into CAFs during carcinogenesis, this reduces the amount of ECM produced and the ECM that ...
Goldberg GI, Wilhelm SM, Kronberger A, Bauer EA, Grant GA, Eisen AZ (May 1986). "Human fibroblast collagenase. Complete primary ... Interstitial collagenase, also known as fibroblast collagenase, and matrix metalloproteinase-1 (MMP-1) is an enzyme that in ... "1.56 A structure of mature truncated human fibroblast collagenase". Proteins. 19 (2): 98-109. doi:10.1002/prot.340190203. PMID ...
The fibroblast growth factor (FGF) family with its prototype members FGF-1 (acidic FGF) and FGF-2 (basic FGF) consists to date ... One of the first applications of pro-angiogenic methods in humans was a German trial using fibroblast growth factor 1 (FGF-1) ... Besides FGF-1, one of the most important functions of fibroblast growth factor-2 (FGF-2 or bFGF) is the promotion of ... Blaber M, DiSalvo J, Thomas KA (February 1996). "X-ray crystal structure of human acidic fibroblast growth factor". ...
They are produced by fibroblasts and monocytes. However, the production of type I IFN-α is inhibited by another cytokine known ... Meager A, Graves H, Burke DC, Swallow DM (August 1979). "Involvement of a gene on chromosome 9 in human fibroblast interferon ... Berthold W, Tan C, Tan YH (June 1978). "Chemical modifications of tyrosyl residue(s) and action of human-fibroblast interferon ... Large amounts of human beta interferon were made by superinducing the beta interferon gene in human fibroblast cells. Cantell's ...
The cell-cell adhesion of fibroblasts is thought to be necessary for wound healing. Mutations in this gene have been shown to ... Protein dachsous homolog 1, also known as protocadherin-16 (PCDH16) or cadherin-19 (CDH19) or cadherin-25 (CDH25) or fibroblast ... This particular cadherin family member is expressed in fibroblasts but not in melanocytes or keratinocytes. ... "Multiple cadherins are expressed in human fibroblasts". Biochem. Biophys. Res. Commun. 235 (2): 355-8. doi:10.1006/bbrc. ...
Kreis, Thomas E.; Birchmeier, Walter (November 1980). "Stress fiber sarcomeres of fibroblasts are contractile". Cell. 22 (2): ... "Identification of Novel Graded Polarity Actin Filament Bundles in Locomoting Heart Fibroblasts: Implications for the Generation ...
Embryonic fibroblasts that are derived from Maff−/−::Mafg−/−::Mafk−/− mice fail to activate Nrf2-dependent cytoprotective genes ...
Fibroblasts are the most common cells of connective tissue in animals. Fibroblasts have a branched cytoplasm surrounding an ... Fibroblasts, like the tumor-associated host fibroblasts (TAF), play a crucial role in immune regulation through TAF-derived ... The life span of a fibroblast, as measured in chick embryos, is 57 ± 3 days. Fibroblasts and fibrocytes are two states of the ... "Fibroblasts". Retrieved 16 August 2018. Weissman-Shomer P, Fry M (1975). "Chick embryo fibroblasts senscence in vitro: pattern ...
Human fibroblasts are reprogrammed to generate blastocyst-like structures called iBlastoids, which recapitulate aspects of ... Here we describe the reprogramming of fibroblasts into in vitro three-dimensional models of the human blastocyst, termed ... which propagated in fibroblast medium with classic fibroblast morphology; n = 2. Scale bars, 100 μm (arrows indicate step-wise ... Liu, X., Tan, J.P., Schröder, J. et al. Modelling human blastocysts by reprogramming fibroblasts into iBlastoids. Nature 591, ...
Researchers have discovered a way to repurpose fibroblasts into functional melanocytes, the bodys pigment-producing cells. The ... New Technology Directly Reprograms Skin Fibroblasts For a New Role. Penn study has implications for new skin disease treatments ... By generating melanocytes from the fibroblasts of melanoma patients, Xu explains, "we can screen not only to find why these ... Newswise - PHILADELPHIA - As the main component of connective tissue in the body, fibroblasts are the most common type of cell ...
Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.. *Cancer-Associated Fibroblasts: Their ... Cancer-associated fibroblasts (CAFs) are a major component of the cancer stroma. CAFs enhance the malignant features of nearby ... Resident tissue fibroblasts, bone marrow-derived mesenchymal stem cells, and hematopoietic stem cells are examples of possible ... Origins of cancer-associated fibroblast. Past studies have reported that many different cells could be the origin of CAFs. ...
Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four ... Direct conversion of fibroblasts to functional neurons by defined factors Nature. 2010 Feb 25;463(7284):1035-41. doi: 10.1038/ ... Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four ... that suffice to rapidly and efficiently convert mouse embryonic and postnatal fibroblasts into functional neurons in vitro. ...
Mouse fibroblasts. Barbara Danowski Affiliation. Union College. Schenectady, New York, USA. Technique. Fluorescence. ...
Here we report that the administration of a set of five small molecules can chemically induce the transformation of fibroblasts ... A set of five small molecules can induce the transformation of fibroblasts into rod photoreceptor-like cells, which can ... We show that mitonuclear communication is a key determining factor for the reprogramming of fibroblasts into CiPCs. ... Chemical reprogramming of fibroblasts offers an opportunity to reverse vision loss; however, the generation of sensory neuronal ...
Ascorbic acid attenuates cell stress by activating the fibroblast growth factor 21/fibroblast growth factor receptor 2/ ... 14 Abstracts with Fibroblast growth factor 21 upregulation Research. Filter by Study Type. Animal Study. ... Pharmacological Actions : Fibroblast growth factor 21 upregulation. Additional Keywords : Gene Expression Regulation, Risk ... Fibroblast growth factor-21 could potentially be used as a hormone therapy to extend lifespan in mammals.Dec 31, 2011. ...
... allogeneic cultured keratinocytes and fibroblasts), frequency-based adverse effects, comprehensive interactions, ... Drugs allogeneic cultured keratinocytes and fibroblasts * 2010gintuit-allogeneic-cultured-keratinocytes-fibroblasts-bovine- ... allogeneic cultured keratinocytes and fibroblasts (Rx). Brand and Other Names:StrataGraft, allogeneic cultured keratinocytes ... encoded search term (allogeneic cultured keratinocytes and fibroblasts (StrataGraft)) and allogeneic cultured keratinocytes and ...
Previous studies have shown that fibroblast growth factor 23 (FGF23), which has an important role in phosphate metabolism, is ... Fibroblast Growth Factor 23 and Left Ventricular Hypertrophy in Hemodialysis Patients () Ayaka Saito1,2, Takako Onuki2, ... Isakova, T. (2013) Fibroblast Growth Factor 23 and Adverse Clinical Outcomes in Chronic Kidney Disease. Current Opinion in ... 2012) Fibroblast Growth Factor 23 and Inflammation in CKD. Clinical Journal of the American Society of Nephrology, 7, 1155-1162 ...
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... Cell. 2007 Nov 30;131(5):861-72. doi: ... Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, ... These findings demonstrate that iPS cells can be generated from adult human fibroblasts. ...
A role for decorin in controlling proliferation, adhesion, and migration of murine embryonic fibroblasts. J Biomed Mater Res A ... Maddaluno L, Urwyler C, Werner S. Fibroblast growth factors: key players in regeneration and tissue repair. Dev Camb Engl. 2017 ... Extracellular Matrix and Dermal Fibroblast Function in the Healing Wound. Adv Wound Care. 2016;5(3):119-36 ... Zoledronic acid and geranylgeraniol regulate cellular behaviour and angiogenic gene expression in human gingival fibroblasts. J ...
Mechanical regulation of cardiac fibroblast profibrotic phenotypes. by Kate M Herum, Jonas Choppe, Aditya Kumar, Adam J Engler ... Mechanical regulation of cardiac fibroblast profibrotic phenotypes.. Molecular biology of the cell. (2017-05-05) ... It occurs as a result of cardiac fibroblast (CFB) activation and differentiation into myofibroblasts, characterized by ... These findings suggest that myocyte stretch, fibroblast stretch, and matrix stiffening following MI may separately regulate ...
... we performed transcriptome-wide RNA-seq and small RNA-seq on synovial fibroblasts from rheumatoid arthritis (RA) subject and ... next-generation sequencing to decipher the molecular mechanisms underlying aberrant rheumatoid arthritis synovial fibroblasts ( ... Synovial Fibroblasts. Primary human synovial fibroblasts isolated from one normal donor and one RA subject were obtained from ... Pap, T.; Müller-Ladner, U.; Gay, R.E.; Gay, S. Fibroblast biology. Role of synovial fibroblasts in the pathogenesis of ...
fibroblast Cell Line. NIH/3T3 Cellular Component. lamellipodium myosin II complex actin filament ... fibroblast. CIL. Dataset. ...
Wildtype mouse embryo fibroblasts were treated with the radiomimetic drug neocarzinostati... ... Wildtype mouse embryo fibroblasts were treated with the radiomimetic drug neocarzinostatin (NCS) for 1hour prior to being fixed ... Jung-Ae Kim, Michael Kruhlak, Farokh Dotiwala, Andre Nussenzweig, James E. Haber (2011) CIL:13408, Mus musculus, fibroblast. ...
CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS. In: Journal of the American Society of Nephrology. 1994 ; ... CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS. Journal of the American Society of Nephrology. 1994;5:496- ... CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS. Claire Loughrey, Paul Jackson, Ann McGinty, Jane McEneny, ... title = "CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS",. author = "Claire Loughrey and Paul Jackson and ...
The cultured fibroblasts were exposed to doses of 100, 250, 500, 750, 1000, 1500, and 3000 rads, and cell numbers were counted ... beta Radiation reduces the proliferation of human Tenons capsule fibroblasts, and at higher doses this effect may be more ... Treatment inhibited the proliferation of the fibroblasts. At seven days the cells exposed to 3000 rads showed a decrease ... of different doses of beta radiation from a strontium-90 source on the proliferation of human Tenons capsule fibroblasts were ...
A Senescence Bystander Effect in Human Lung Fibroblasts Waters, David W.; Schuliga, Michael; Pathinayake, Prabuddha S.; Wei, ... In this study, we investigated whether senescent human lung fibroblasts (LFs) and alveolar epithelial cells (AECs) could induce ... In this study, we investigated whether senescent human lung fibroblasts (LFs) and alveolar epithelial cells (AECs) could induce ... IPF and cellular senescence is well established and several studies now describe a higher abundance of senescent fibroblasts ...
Neuroscience research articles are provided.. What is neuroscience? Neuroscience is the scientific study of nervous systems. Neuroscience can involve research from many branches of science including those involving neurology, brain science, neurobiology, psychology, computer science, artificial intelligence, statistics, prosthetics, neuroimaging, engineering, medicine, physics, mathematics, pharmacology, electrophysiology, biology, robotics and technology. ...
J:29471 Ruta M, et al., Receptor for acidic fibroblast growth factor is related to the tyrosine kinase encoded by the fms-like ...
H2O2-treated skin fibroblasts (n=4, **P,0.01, ***P,0.001). (B) Skin fibroblast apoptosis in control, H2O2-treated (300 µM), 200 ... A) Human skin fibroblasts were cultured in the presence of TFPS (0, 10, 20, 50, 100, 200, and 300 µg/ml), for 24 h, and cell ... However, human skin fibroblasts pretreated with 200 µg/ml TFPS had attenuated H2O2-induced ROS generation, with a decline of ... Human skin fibroblasts (5,000 cells/well) were plated onto 24-well plates, pretreated with TFPS for 1 h and then treated with ...
The mitochondrial function and glycolytic function in MERRF skin fibroblasts were also evaluated by the XF Analyzer as compared ... In this webinar we will discuss our research regarding energy metabolism within the primary cultures of skin fibroblasts from ... Assessment of metabolic reprogramming of the skin fibroblasts from patients with mitochondrial diseases using the Seahorse XF ... Assessment of metabolic reprogramming of the skin fibroblasts from patients with mitochondrial diseases using the Seahorse XF ...
mouse fibroblasts ; human keratinocytes ; Staphylococcus aureus ; atopic dermatitis ... NC/Nga mouse fibroblasts that lacked soluble IL-33 (sST2) receptor were more sensitive to the effects of S. aureus than control ... Methods: Human keratinocytes (HEKa), and mouse embryonic fibroblasts (MEF) from the NC/Nga dermatitis prone mouse strain were ... Mechanisms by which Staphylococcus aureus induces cytokines and cell death in human keratinocytes and mouse fibroblasts ...
Fibroblast growth factor 21 (FGF21) is a metabolic hormone with multiple beneficial effects on lipid and glucose homeostasis. ... S. Jiang, C. Yan, Q.-C. Fang et al., "Fibroblast growth factor 21 is regulated by the IRE1α-XBP1 branch of the unfolded protein ... Identification of Sp1 as a Transcription Activator to Regulate Fibroblast Growth Factor 21 Gene Expression. Shuqin Chen. ,1 ... Fibroblast growth factor 21 (FGF21), an atypical member of the FGF family, functions as a hormone that coordinates lipid and ...
The current study was undertaken to uncover the role of melatonin in lipid metabolism in the murine fibroblasts. The results ... However, low levels of melatonin, with or without oleic acid, did not influence lipid metabolism in the cultured fibroblasts. ... It appears that the effects of melatonin on lipid metabolism in murine fibroblasts is mediated by melatonin membrane receptors. ... Maldonado A, Siu N, Sánchez-Hidalgo A, Acuna-Castroviejo A, Escames E, Melatonin and lipid uptake by murine fibroblasts: ...
... such as synovial fibroblasts (FLS), despite their crucial involvement in the pathogenesis of RA. Here we integrate DNA ... From: Functional genomics atlas of synovial fibroblasts defining rheumatoid arthritis heritability. Locus Chr SNP cate gory FLS ...
  • Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. (
  • Methods RASF, rheumatoid arthritis dermal fibroblasts (RADF) and osteoarthritis synovial fibroblasts (OASF) were analysed for the expression of B cell survival/proliferating factors BAFF and APRIL in resting conditions and upon stimulation with TLR2/TLR3/TLR4 ligands. (
  • Rat Dermal Fibroblasts (RDF) provided by Innoprot are isolated from Sprague Dawley rat skin by explants. (
  • Each vial of Rat Dermal Fibroblasts contains more than 500.000 viable cells. (
  • Innoprot also offers optimized medium and reagents for the growth of Rat Dermal Fibroblasts which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro RDF culture. (
  • Cultures of rat dermal fibroblasts are very useful for a wide range of cellular and molecular studies. (
  • Dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. (
  • During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype. (
  • There have been few studies to date of the phenotype of dermal fibroblasts in the presence of glyoxal, and our research shows that AGEs alter key properties of fibroblasts. (
  • For the first time, we show that glycation can induce ectopic lipid accumulation through increased expression of PLIN2 in dermal fibroblasts, independently of the AMPK pathway. (
  • In this study, we investigate the expression and the role of HDACs on the senescent phenotype of dermal fibroblasts. (
  • Moreover, treatment with SAHA, a histone deacetylase inhibitor (HDACi) also known as Vorinostat, or the specific downregulation of HDAC2 or HDAC7 by siRNA, induces the appearance of senescence biomarkers of dermal fibroblasts. (
  • Conversely, the ectopic re-expression of HDAC7 by lentiviral transduction in pre-senescent dermal fibroblasts extends their proliferative lifespan. (
  • SpinalCyte, a Texas-based tissue engineering technology company focused on regrowth of the spinal disc nucleus using human dermal fibroblasts, has been issued a new. (
  • Background: There is little information on specific interactions between dermal fibroblasts and epidermal keratinocytes. (
  • Human Dermal Fibroblast-adult Cell Pellet (HDF-a Cell Pellet) is prepared from early passage Human Dermal Fibroblasts-adult. (
  • Transcriptomics evaluation of hexavalent chromium toxicity in human dermal fibroblasts. (
  • Therefore, maintaining the population of dermal fibroblasts is important for both preventing and treating age-related skin changes. (
  • Using next-generation sequencing to decipher the molecular mechanisms underlying aberrant rheumatoid arthritis synovial fibroblasts (RASF) activation, we performed transcriptome-wide RNA-seq and small RNA-seq on synovial fibroblasts from rheumatoid arthritis (RA) subject and normal donor. (
  • In the present study, we found that Kirenol inhibited the migration, invasion, and proinflammatory of IL-6 secretion of RA-associated synovial fibroblasts (FLS) at a concentration of 100-200 μg/ml in vitro . (
  • Synovial fibroblasts (FLS) promote joint destruction via their attachment to the cartilage, and thus are key mediators of the pathogenesis of RA ( 2 ). (
  • BCP crystals increase prostacyclin production and upregulate the prostacyclin receptor in OA synovial fibroblasts: potential effects on mPGES1 and MMP-13. (
  • Mechanism of basic calcium phosphate crystal-stimulated cyclo-oxygenase-1 up-regulation in osteoarthritic synovial fibroblasts. (
  • miR-146a overexpression decreases TRAF6 protein expression levels in primary human synovial fibroblasts. (
  • B ) Western blot analysis showing significant decrease in the protein expression levels of TRAF6 in miR-146a overexpressed primary human synovial fibroblasts compared to controls and scrambled miR-146a. (
  • Objectives To dissect the role of toll-like receptor (TLR) signalling and B cell survival/proliferating factors in the crosstalk between rheumatoid arthritis synovial fibroblasts (RASF) and B cells. (
  • During osteoarthritis, synovial fibroblasts exposed to anomalous mechanical forces and an inflammatory microenvironment release factors such as a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) metalloproteinases that mediate tissue damage and perpetuate inflammation. (
  • We therefore studied the production of ADAMTS by synovial fibroblasts and their contribution to cartilage degradation. (
  • We reported that synovial fibroblasts constitutively express and release ADAMTS 4, 5, 7, and 12. (
  • Therefore, synovial fibroblasts provide the biochemical tools to the chronicity and destruction of the osteoarthritic joints. (
  • In this study, we found that gene GGT5 was highly expressed in cancer-associated fibroblasts (CAFs) in lung adenocarcinoma, predicting the unfavorable survival of patients with lung adenocarcinoma. (
  • The PDAC tumor microenvironment (TME) is characterized by a dense desmoplastic stroma, dominated by cancer-associated fibroblasts (CAF), extracellular matrix (ECM) and immune cells displaying immunosuppressive phenotypes. (
  • We postulated that cancer associated fibroblasts (CAFs) within the tumor stroma may exhibit subtype specific gene expression profiles and thus contribute to the biology of the disease in a subtype specific manner. (
  • However, breast cancer subtype-specific differences have not yet been reported for the tumor stromal cells even though multiple studies have shown that the gene expression profiles of breast cancer associated fibroblasts (CAFs) are distinctly different from their normal counterparts. (
  • Moreover, we discuss the impact of the cells responsible for this altered stromal collagen remodeling, the cancer associated fibroblasts (CAFs), and how these cells are key players in orchestrating the tumor microenvironment composition and tissue microarchitecture, hence also driving tumorigenesis and affecting response to treatment. (
  • The goal of this study is to investigate the role of cancer-associated fibroblasts and an extracellular matrix hyaluronic acids in the enhancement of metastatic liver tumor growth in ALD. (
  • Starting from a pool of nineteen candidate genes, we identified a combination of only three factors, Ascl1, Brn2 (also called Pou3f2) and Myt1l, that suffice to rapidly and efficiently convert mouse embryonic and postnatal fibroblasts into functional neurons in vitro. (
  • Methods: Human keratinocytes (HEKa), and mouse embryonic fibroblasts (MEF) from the NC/Nga dermatitis prone mouse strain were used to investigate the induction of Th2-promoting cytokines (IL-33 and TSLP) and cell death by S. aureus. (
  • Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy. (
  • By examining markers of different signaling pathways, and the differentiation behaviors of cells in the early embryonic brain, we infer that Gpc1(-/-) phenotypes most likely result from a transient reduction in fibroblast growth factor (FGF) signaling. (
  • Embryonic mouse fibroblasts (NIH‐3T3) spread on all arrays, and on contact with the substrate engulf nanopillars independently of the array pitch. (
  • Derived from day 12.5 post coitus fetuses of strain ICR mice and cultured as a monolayer in OriCell TM Mouse Embryonic Fibroblast Growth Medium (Cat. (
  • The ED50 as determined in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells is less than 3 ug/ml. (
  • Here we describe the reprogramming of fibroblasts into in vitro three-dimensional models of the human blastocyst, termed iBlastoids. (
  • The results of this in vitro study indicate that a very low BP dose (10 -9 M) can significantly affect the physiology of fibroblasts, increasing their proliferative capacity and modulating the expression of multiple genes involved in their growth and differentiation. (
  • In this study, we investigated whether senescent human lung fibroblasts (LFs) and alveolar epithelial cells (AECs) could induce a senescent-like phenotype in "naïve" non-senescent LFs in vitro. (
  • As in vivo analysis of human pulmonary fibroblasts is not possible, the current authors designed an in vitro model of the local cytokine microenvironment of the diseased lung, using supernatants of stimulated epithelial and CD8+ T-cells. (
  • The aim of the pilot study was the evaluation of the keratinised gingival fibroblast proliferation process after the use of Protefix® gel in vitro . (
  • An in vitro pilot study of human fibroblasts obtained from healthy donors and isolated from keratinised gingival tissues. (
  • In this in vitro pilot study, it was shown that Protefix® gel significantly stimulated the proliferation of human fibroblasts from primary culture. (
  • To evaluate the role of this transcription factor in cardiac fibroblasts (CFs) in diabetes mellitus, we performed a series of in vitro and in vivo experiments. (
  • We determined the local composition and mechanical properties of the myocardium in established mouse models of genetic and acquired fibrosis and tested the effect of myocardial composition on cardiomyocyte contractility in vitro by systematically manipulating the number of fibroblasts and collagen concentration in a platform of engineered cardiac microtissues. (
  • The in vitro results showed that while increasing collagen content had little effect on microtissue contraction, increasing fibroblast density caused a significant reduction in contraction force. (
  • In vitro studies were then pursued with cultured isolated cardiac fibroblasts. (
  • In order to determine if mononuclear cells may be secreting factors capable of modulating fibroblast growth, the in vitro proliferative response of fibroblasts to cytokines known to be secreted by mononuclear cells was measured, using both growth arrested and proliferating cells. (
  • A fibroblast is a type of biological cell that synthesizes the extracellular matrix and collagen, produces the structural framework (stroma) for animal tissues, and plays a critical role in wound healing. (
  • The main function of fibroblasts is to maintain the structural integrity of connective tissues by continuously secreting precursors of the extracellular matrix. (
  • Fibroblasts secrete the precursors of all the components of the extracellular matrix, primarily the ground substance and a variety of fibers. (
  • Fibroblasts, like the tumor-associated host fibroblasts (TAF), play a crucial role in immune regulation through TAF-derived extracellular matrix (ECM) components and modulators. (
  • It occurs as a result of cardiac fibroblast (CFB) activation and differentiation into myofibroblasts, characterized by proliferation, extracellular matrix (ECM) production and stiffening, and contraction due to the expression of smooth muscle α-actin. (
  • Fibroblasts are responsible for forming and maintaining soft connective tissue and constitute the main source of collagen for the extracellular matrix (ECM). (
  • Especially adhesion-related genes are upregulated in fibroblasts of chronic obstructive pulmonary disease patients, which can indicate a more pronounced role of fibroblasts in the inflammatory process in chronic obstructive pulmonary disease, possibly resulting in reduced function as effectors of extracellular matrix repair. (
  • Fibroblasts are important in tissue repair and modulation by producing extracellular matrix (ECM) proteins, ECM-degrading enzymes and their inhibitors 9 . (
  • Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. (
  • Fibrosis is mediated partly by extracellular matrix-depositing fibroblasts in the heart. (
  • We investigated the effect of its ethanol extract on hyaluronan (HA), an extracellular matrix compound with proinflammatory activity synthesis in human oral fibroblasts. (
  • Fibroblasts are responsible for maintaining the extracellular matrix in healthy hearts, and differentiate into the wound-healing phenotype, myofibroblasts, following cardiac injury (3). (
  • Cancer-associated fibroblast (CAF) is a component of tumor microenvironment and can produce extracellular matrix (ECM), which could play an important role in cancer growth, invasion, and metastasis. (
  • A cardiac fibroblast is a connective tissue cell in the heart which secretes an extracellular matrix rich in collagen and other macromolecules. (
  • Fibroblasts play a key role in the production of these extracellular matrix components in the skin. (
  • Skin aging is the consequence of reduced numbers of fibroblasts, lower levels of extracellular matrix proteins, and decreased skin elasticity and tonus, thereby resulting in the formation of wrinkles[ 2 ]. (
  • Fibroblasts are the most common cells of connective tissue in animals. (
  • Fibroblasts can also migrate slowly over substratum as individual cells, again in contrast to epithelial cells. (
  • While epithelial cells form the lining of body structures, it is fibroblasts and related connective tissues which sculpt the "bulk" of an organism. (
  • Fibroblasts and fibrocytes are two states of the same cells, the former being the activated state, the latter the less active state, concerned with maintenance and tissue metabolism. (
  • Like other cells of connective tissue, fibroblasts are derived from primitive mesenchyme. (
  • In certain situations, epithelial cells can give rise to fibroblasts, a process called epithelial-mesenchymal transition (EMT). (
  • Receptors on the surface of fibroblasts also allow regulation of hematopoietic cells and provide a pathway for immune cells to regulate fibroblasts. (
  • Induction of pluripotent stem cells from adult human fibroblasts by defined factors. (
  • Cancer cells that were cultured with CAFs had down-regulated E-cadherin expression compared to cancer cells that were cultured with normal fibroblasts. (
  • Resident tissue fibroblasts, bone marrow-derived mesenchymal stem cells, and hematopoietic stem cells are examples of possible predecessors of CAFs. (
  • One method involves cancer cells reprogramming resident tissue fibroblasts to become CAFs, using small non-coding RNA molecules known as microRNAs or miRNAs (such as miR-31 and miR-214). (
  • Finally, bone marrow-derived mesenchymal stem cells (BM-MSCs) can alter secreted transforming growth factor-β1 (TGF-β1), which promotes the formation of CAFs from fibroblasts. (
  • Here we report that the administration of a set of five small molecules can chemically induce the transformation of fibroblasts into rod photoreceptor-like cells. (
  • These findings demonstrate that iPS cells can be generated from adult human fibroblasts. (
  • Human CCD-1064Sk epithelial fibroblast cells were incubated in culture medium with 10 -5 , 10 -7 , or 10 -9 M zoledronate, alendronate, or ibandronate. (
  • Fibroblasts proliferation was significantly increased at the lowest dose (10 -9 M) of each BP but was not affected at the higher doses (10 -5 and 10 -7 M). The proliferation increase may be related to the rise in TGF-β1 and TGFβR1 expression detected after the treatment of cells with 10 -9 M of zoledronate, alendronate, or ibandronate. (
  • An association between IPF and cellular senescence is well established and several studies now describe a higher abundance of senescent fibroblasts and epithelial cells in the lungs of IPF patients compared with age-matched controls. (
  • We compare testosterone (T) metabolism in primary cultures of epithelial cells and fibroblasts separated from benign prostate hypertrophy (BPH) and prostate cancer tissues. (
  • The amounts of 5 alpha-dihydrotestosterone (DHT), formed by reduction of T by 5 alpha-reductase (5 alpha-R), were small: 5 and 2% (BPH) and 8 and 15% (adenocarcinoma) for epithelial cells and fibroblasts, respectively. (
  • The 5 alpha-reductase inhibitors, 4-MA (17 beta-(N,N-diethyl)carbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one) and finasteride, inhibited DHT formation with a preferential action of 4-MA on epithelial cells (BPH or adenocarcinoma) and of finasteride on fibroblasts from adenocarcinoma. (
  • On the other hand, the lipido-sterol extract of Serenoa repens (LSESr, Permixon) inhibited the formation of all the T metabolites studied [IC50 S = 40 and 200 micrograms/ml (BPH) and 90 and 70 micrograms/ml (adenocarcinoma) in epithelial cells and fibroblasts, respectively]. (
  • In this study, we examined the sub-membrane mechanical structures of normal fibroblasts TIG-1 cells, and cervical cancer Hela cells using local elasticity mapping method of atomic force microscope. (
  • In addition to ECM production, fibroblasts can be actively involved in the inflammatory processes via cytokine and chemokine production and by direct interaction with inflammatory cells 7 , 17 , 18 . (
  • The data suggest that the malignant state of human fibroblasts is always associated with high levels of activity of receptor-bound u-PA, and in addition cells transformed to the malignant state are very likely to exhibit high levels of receptorbound t-PA and secreted forms of plasminogen activators. (
  • While untreated cells appeared fibroblast -like ( spindle or bundle shape), the alignment of exposed cells was altered into irregular polygonal configurations. (
  • This is mainly because fibroblasts are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. (
  • Carcinoma-associated fibroblasts (CAFs) are abundant stromal cells in tumor microenvironment that are critically involved in cancer progression. (
  • Analysis of the cell states revealed different transcriptional programs in luminal epithelial cells (hormone receptor positive and secretory), basal epithelial cells (myoepithelial or basement-like), endothelial cells (lymphatic or vascular), macrophages (M1 or M2) and fibroblasts (three subgroups) and provided insight into progenitors of each cell types, she said. (
  • For example, the proportion of fibroblasts was lower in 3 of the 11 patients, and even though the cells were pathologically normal, immune cell populations, including T-cells and macrophages, were also seen. (
  • Results We found that the proliferative and migratory capacities of the cells were greatly reduced by glycation, which could be explained by an increase in fibroblast tensile strength. (
  • Growth stimulatory factor originally isolated from bovine brain and pituitary and found to stimulate DNA synthesis in cultured fibroblast cells. (
  • The increase of the inhibiting action of shaken serum on homologous fibroblasts is due possibly to the disappearance of a substance favoring the activity of homologous cells. (
  • Exposure to hemin increased the gene and protein expression level of HO-1 in fibroblasts and diminished ROS levels, senescence, the inflammatory profile and simultaneously rescued mitochondria dysfunction by restoring mitophagy in COPD cells. (
  • We showed that Thy1(+)CD45(-)CD31(-)CD11b(-)Ter119(-) cells constitute the majority of cardiac fibroblasts. (
  • We used lineage tracing, transplantation studies, and parabiosis to show that most adult cardiac fibroblasts derive from the epicardium, a minority arises from endothelial cells, and a small fraction from Pax3-expressing cells. (
  • We did not detect generation of cardiac fibroblasts by bone marrow or circulating cells. (
  • In this study, it was investigated whether the expression of Thy-1 (CD90), a member of the immunoglobulin superfamily of adhesion molecules with constitutive expression on fibroblast cells, is modulated following infection with HCMV. (
  • Interestingly, after infection of fibroblasts with a recombinant GFP-expressing virus, infected as well as non-infected cells showed a reduced Thy-1 cell surface expression. (
  • Isolated from human kidney biopsies, these fibroblasts are adherent-dependent cells cultured on gelatin-precoated flasks and are transported in adherent form only. (
  • Several years ago, a postdoctoral researcher working in Fu's lab was taking that approach, using a technique called siRNA to silence the PTB gene in connective tissue cells known as fibroblasts. (
  • We aimed to optimize a flow cytometric method described by other workers using endothelial cells to better differentiate between populations of fibroblasts in degrees of SA-β-gal activity. (
  • Freshly dissociated fibroblasts were exposed to 30, 60 or 120 minutes of ischaemia by coating pelleted cells with mineral oil (modified from 5), followed by 60 minutes of reperfusion when mineral oil was replaced with culture media. (
  • IR of all durations evoked significant differentiation of fibroblasts into myofibroblasts, with 88±1% (mean±S.E.M., n=5) of cells differentiating following 30 minutes of ischaemia, 93±2% (n=4) following 60 minutes of ischaemia, and 92±1% (n=5) following 120 minutes of ischaemia. (
  • of cells differentiated from fibroblasts into myofibroblasts without IR. (
  • Fibroblasts are mesenchymal cells which perform many vital functions during development and in adulthood. (
  • Fibroblasts are one of the most accessible mammalian cell types and one of the easiest types of cells to grow in culture. (
  • By gently exfoliating the skin with electrostatic energy, the Dermavel Plasma Pen stimulates collagen and protein production in the fibroblast cells of the skin. (
  • The cytotoxicity effects for human fibroblasts were then compared with those reported for HCT 116 cells, and the findings point out that it is relevant to consider the cellular size. (
  • Somatic mosaicism of an intragenic FANCB duplication in both fibroblast and peripheral blood cells observed in a Fanconi anemia patient leads to milder phenotype. (
  • Fibroblasts are the most common biological cells that develop and maintain connective tissue in the body. (
  • Gingival fibroblasts (GFs) present in the bone-lining mucosa have the capacity to activate the formation of osteoclasts, but little is known about which local immune cells (co-)mediate this process. (
  • After 21 days, comparable numbers of multinucleated cells (osteoclasts) were found in gingival fibroblast (GF)-PBMC and GF-monocyte cocultures. (
  • Fibroblasts constitute a dynamic and versatile population of cells of mesenchymal origin, implicated in both regenerative strategies and pathological conditions. (
  • This review intends to highlight the potential of fibroblasts in orchestrating tissue regeneration, as well as to contribute to uncover uncharted prospective applications of these cells. (
  • Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which sµggested a role in testicular embryogenesis Fibroblast Growth Factor 9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. (
  • Furthermore, by developing a fibroblast stem cells (FSC)-enriched fibroblast focus model to mimic in vivo fibrogenic response, we demonstrated a dose-dependent increase in fibroblast focus formation and collagen production by primary lung fibroblasts treated with multi-walled carbon nanotubes (MWCNTs). (
  • In this study, we investigated the viability of human lung fibroblast-derived (HFL1) cells to different concentrations of cigarette smoke extract (CSE). (
  • GBA1 activities in both total lysate and PM of GD fibroblasts were low, and their relative percentages were similar to those of control cells. (
  • The current study demonstrated that iPSC reprogramming in N2B27+2i/LIF culture converted chimpanzee fibroblasts into a multipotent cancerous state with unique gene expression, but not fully pluripotent stem cells. (
  • Plasma fibroblast therapy is a noninvasive way to stimulate the cells that produce collagen within your skin, called fibroblasts. (
  • The plasma fibroblast treatment uses naturally occurring plasma cells to break down the bonds between collagen and elastin in the skin. (
  • Conversely, fibroblasts in some situations may give rise to epithelia by undergoing a mesenchymal to epithelial transition (MET) and organizing into a condensed, polarized, laterally connected true epithelial sheet. (
  • Lung fibroblasts from smokers and COPD patients displayed in long-term culture a senescent phenotype, characterized by a reduced replicative capacity, an increased senescence and inflammatory profile. (
  • These parameters were significantly higher in senescent COPD fibroblasts which also exhibited decreased mitochondrial activity (respiration, glycolysis, and ATP levels) which led to an increased production of ROS, and mitochondria biogenesis and impaired mitophagy process. (
  • The impact of senescent fibroblasts was further investigated in a pilot study using radiofrequency (RF) intervention for melasma. (
  • This leads us to the speculation that senescent fibroblasts may contribute to drive melasma and might be considered as a potential therapeutic target. (
  • Secretion of leukotrienes by senescent lung fibroblasts promotes pulmonary fibrosis. (
  • The LT-rich CM of senescent lung fibroblasts (IMR-90) induced profibrotic signaling in naive fibroblasts, which were abrogated by inhibitors of ALOX5, the principal enzyme in LT biosynthesis. (
  • Unlike human fibroblasts from unused donor lungs made senescent by irradiation, senescent IPF fibroblasts secreted LTs but failed to synthesize PGs. (
  • Conclusions: S. aureus is unique amongst staphylococcal species in it's ability to induce an inflammatory response and cytotoxicity in human keratinocytes and mouse fibroblasts. (
  • Research design and methods To investigate this, we treated cultured human fibroblasts with 0.6 mM glyoxal to induce acute glycation. (
  • Background: To investigate whether monosodium urate (MSU) crystals induce interleukin (IL)-1β in human fibroblast-like synoviocytes (FLS), and whether the NLRP3 inflammasome is involved in the inflammatory mechanism. (
  • ROS production, known to induce senescence, is increased in COPD fibroblasts and mitochondria dysfunction participates in this process. (
  • We hypothesized that basic fibroblast growth factor might induce a more aggressive phenotype dependent on the amount of protein expressed in melanoma. (
  • To induce stress-induced senescence, fibroblasts were exposed to rotenone. (
  • A synthetic elastase inhibitor failed to induce HGF, but α 1 -antitrypsin also stimulated HGF production in lung fibroblasts. (
  • G can have very low GAA activity in fibroblasts but express higher activity in muscle and store less glycogen in muscle than patients with classic-infantile Pompe disease. (
  • Under stress-induced conditions the flow cytometric method but not the cytochemical method revealed significant higher SA-β-gal activity in fibroblasts from very old compared to young subjects (P = 0.004 and P = 0.635 respectively). (
  • We studied the effects of two imino sugars, deoxynojirimycin (DNJ) and N-butyldeoxynojirimycin (NB-DNJ), on residual GAA activity in fibroblasts from eight patients with different forms of Pompe disease (two classic infantile, two non-classic infantile onset, four late-onset forms), and with different mutations of the GAA gene. (
  • Mechanical regulation of cardiac fibroblast profibrotic phenotypes. (
  • Human Cardiac Fibroblast-adult ventricular total RNA (HCF-av tRNA) is prepared from early passage Human Cardiac Fibroblasts-adult ventricular using the Qiagen AllPrep DNA/RNA Mini kit. (
  • We performed the following studies to examine whether MBG might directly stimulate cardiac fibroblast collagen production. (
  • The process in which a relatively unspecialized cell acquires the specialized structural and/or functional features of a cardiac fibroblast. (
  • A Senescence Bystander Effect in Human Lung Fibroblasts Waters, David W. (
  • The current authors explored the effects of the cytokine microenvironment on cell-cell interaction gene expression in pulmonary fibroblasts of controls (n = 6), and Global Initiative for Chronic Obstructive Lung Disease stage II (n = 7) and stage IV (n = 7) COPD patients. (
  • The present data show that fibroblasts of chronic obstructive pulmonary disease patients display an altered response to the cytokine microenvironment, depending on both the disease stage and the central or peripheral location in the lung. (
  • The current authors have previously observed that lung fibroblasts of patients with severe COPD exhibit an altered ECM production profile compared with fibroblasts of controls pointing to an important role of fibroblasts in the defective ECM modulation observed in COPD 8 , 10 - 12 . (
  • The current study was conducted to further explore the role of fibroblasts in COPD and especially to investigate the influence of the complex cytokine microenvironment of the diseased lung on cell-to-cell interaction and matrix production. (
  • Characterization of Distinct Populations of Carcinoma-Associated Fibroblasts from Non-Small Cell Lung Carcinoma Reveals a Role for ST8SIA2 in Cancer Cell Invasion. (
  • Chronic obstructive pulmonary disease (COPD) is associated with lung fibroblast senescence, a process characterized by an irreversible proliferation arrest associated with secretion of inflammatory mediators. (
  • Fibroblasts from smoker controls (S-C) and COPD patients were isolated from lung biopsies. (
  • Rat neonatal lung fibroblasts were isolated from the lung of adult SD rats. (
  • Here we report the positive regulation of hepatocyte growth factor (HGF) production in human lung fibroblasts exerted by SLPI or its C-terminal domain under physiologic concentrations (1 to 10 μM). (
  • In contrast, human skin fibroblasts exerted no SLPI-stimulated increase in HGF production, despite the fact that IL-1β increased HGF production with an intensity similar to that of human lung fibroblasts. (
  • Both the time-course and dose-response studies in human lung fibroblasts revealed that the induction of HGF messenger RNA (mRNA) and protein occurred in parallel, indicating that the mechanism existed at the steady-state mRNA level. (
  • The induced proteins were identified as procollagenases by immunoprecipitation of induced medium with antibodies to purified human fibroblast collagenase. (
  • We analyzed the activities of CBE-sensitive β-glucosidase (GBA1) and AMP-DNM-sensitive β-glucosidase (GBA2) in total cell lysates and PM of human fibroblast cell lines from control (normal) subjects and from patients with GD clinical types 1, 2, and 3. (
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor 11 (FGF11) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fibroblast Growth Factor 11 (FGF11) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (
  • Continued accumulation and activity of fibroblasts along with differentiation into myofibroblasts, however, results in thickened, dense bands of highly aligned fibers. (
  • IPC produced by 15 minutes ischaemia and 30 minutes reperfusion, prior to 60 minutes of ischaemia and 60 minutes of reperfusion, significantly reduced the differentiation of fibroblasts into myofibroblasts from 88±2% (n=7, no IPC) to 46±4% (n=7, IPC)(Fig. 1). (
  • These data indicate that IR injury strongly stimulates differentiation of cardiac fibroblasts into the wound-healing phenotype, the myofibroblast. (
  • IPC prevented IR-induced differentiation in a glibenclamide-sensitive manner, suggesting that activation of KATP channels is part of the mechanism by which IPC protects cardiac fibroblasts. (
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fibroblast Growth Factor 11 (FGF11) in samples from serum, plasma or other biological fluids. (
  • We tested 3 different targets, namely fibroblast activation protein (FAP), macrophages, and integrin α v β 3 . (
  • Importantly, and in agreement with the recombinant protein work, an interaction between CaMKII and IKKβ was evident following Proximity Ligation Assays in adult cardiac fibroblasts. (
  • A protein called fibroblast growth factor 22 (or FGF22 for short) helps synapses to initially form within the hippocampus. (
  • We have demonstrated the effects of retinoic acids of all-trans retinoic acid and 9-cis retinoic acid on the cell growth, alkaline phosphatase (ALPase) activity and protein synthesis of human deciduous and permanent periodontal ligments derived fibroblasts (HPLF-Y and HPFL,respectively). (
  • The data document that the killing of replicating mouse fibroblasts by etoposide represents an example of programmed cell death (apoptosis) that depends on protein synthesis. (
  • Fibroblast activity is enhanced by increases in store-operated Ca2+ entry (SOCE) and calcium release-activated calcium channel protein 1 (Orai1) levels. (
  • The main structural protein secreted by fibroblasts is collagen which helps to minimise fine lines, micro-furrows and wrinkles on the face and improves the elasticity, suppleness and hydration of our skin. (
  • Heufelder, AE, Wenzel, BE & Bahn, RS 1992, ' Cell surface localization of a 72 kilodalton heat shock protein in retroocular fibroblasts from patients with Graves' ophthalmopathy ', Journal of Clinical Endocrinology and Metabolism , vol. 74, no. 4, pp. 732-736. (
  • Bahn, Rebecca S. / Cell surface localization of a 72 kilodalton heat shock protein in retroocular fibroblasts from patients with Graves' ophthalmopathy . (
  • Lyophilized Fibroblast Growth Factor-9 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF9 Mouse Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. (
  • Fibroblast activation protein-alpha and dipeptidyl peptidase IV (CD26): cell-surface proteases that activate cell signaling and are potential targets for cancer therapy. (
  • fibroblasts (CAFs) are a major component of the cancer stroma. (
  • The conversion of CAFs from normal fibroblasts can occur in many ways. (
  • More recent research has discovered that the conversion of normal fibroblasts into CAFs involves the alteration of p53 from tumor-suppressive actions to tumor-supportive actions. (
  • Several studies have reported gene expression profile differences between CAFs and normal breast fibroblasts but in none of these studies were the results stratified based on tumor subtypes. (
  • The keratinocyte growth factor receptor or fibroblast growth factor receptor 2b (KGFR/FGFR2b) is activated by the specific interaction with the keratinocyte growth factor (KGF/FGF7), which targets the receptor to the degradative pathway, and the fibroblast growth factor 10 (FGF10/KGF2), which drives the receptor to the juxtanuclear recycling route. (
  • Manzano-Moreno FJ, Illescas-Montes R, Melguizo-Rodriguez L, Costela-Ruiz VJ, García-Martínez O, Ruiz C, Ramos-Torrecillas J. Impact of bisphosphonates on the proliferation and gene expression of human fibroblasts. (
  • To determine differences in gene expression among CD26+ and CD26- fibroblasts from irradiated and non-irradiated breast capsules. (
  • Furthermore, RNA-seq analysis demonstrated that Tie2-derived and Tbx18-derived fibroblasts within each operation group exhibit similar gene expression profiles. (
  • Though morphologically inconspicuous, ectopically transplanted fibroblasts can often retain positional memory of the location and tissue context where they had previously resided, at least over a few generations. (
  • Tissue damage stimulates fibrocytes and induces the production of fibroblasts. (
  • citation needed] Besides their commonly known role as structural components, fibroblasts play a critical role in an immune response to a tissue injury. (
  • To determine whether a relationship exists among urokinase plasminogen activator (u-PA) activity, tissue plasminogen activator (t-PA) activity, and the malignant transformation of human fibroblasts, we measured receptor-bound and secreted u-PAs and t-PA activity in fibroblast cell strains of a unique cell lineage and compared the results with the values obtained in human fibrosarcoma-derived cell lines and control cell lines. (
  • GAA activity and glycogen content in fibroblasts and muscle tissue in healthy controls, adult patients with Pompe disease and classic-infantile patients were compared with those of the three index patients. (
  • We found a fibroblast-like cell group composed of 6 clusters, 2 of which were validated for their enrichment in Aromhum LAM tissue. (
  • Human Liver Fibroblasts are isolated from normal human liver tissue. (
  • Human Liver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Culture Complete Growth Medium generally for 3-7 days. (
  • Gingival fibroblasts are the major constituents of gingival tissue and play a key role in their maintenance. (
  • When Plasma fibroblast is carried out by a trained professional in a clinical environment, it can provide skin tightening effects, improve skin texture, and encourage tissue regeneration. (
  • Despite being frequently associated to disease development, particularly through the establishment of fibrotic tissue, fibroblasts hold great potential for tissue engineering and regenerative medicine applications. (
  • Histologic studies have shown an accumulation of macrophages, lymphocytes, and fibroblasts, the latter of which lay down multi-directional collagen fibers during initial capsule formation. (
  • IL-1β, TNFα produced by macrophages, IL-6 produced by fibroblasts). (
  • In human foreskin fibroblast cultures two proteins with M(r) 60,000 and 55,000 were found to be induced about 3.5-fold by epidermal growth factor (EGF), platelet-derived growth factor, and β-transforming growth factor. (
  • Two human melanoma cell lines, M14 and 1F6, known to have low endogenous basic fibroblast growth factor expression and slow growth as subcutaneous xenografts, were stably transfected with vectors encoding either the 18 kDa or all (ALL) isoform proteins of human basic fibroblast growth factor. (
  • Different clones overexpressing the 18 kDa or ALL basic fibroblast growth factor proteins were easily obtained. (
  • When the micro-damage occurs fibroblasts near the wound will multiply, travel to the site of damage and secrete collagen and other proteins to isolate the injury and facilitate wound repair. (
  • Because several other parkinsonism-associated proteins have been connected to mitochondrial function and mitophagy, we studied the impact of endogenous mutations in ATPase type 13A2 (ATP13A2) on mitochondria in fibroblasts from KRS patients compared with controls. (
  • Cell surface proteins of NIL1 hamster fibroblasts labeled by a galactose oxidase, tritiated borohydride method. (
  • METHODS: Human cultured fibroblasts of three different donors and three different short-term human lymphocyte cultures were exposed to 1,950 MHz UMTS below the specific absorption rate (SAR) safety limit of 2 W/kg. (
  • Methods: DNA was sequenced and GAA activity and glycogen content were measured in leukocytes, fibroblasts and muscle. (
  • Methods: The effects of basic fibroblast growth factor on flexor tendon healing were evaluated with use of a canine model. (
  • Methods: Human keratinocytes were seeded on collagen matrices populated with increasing numbers of fibroblasts and cultured for 2 weeks at the air-liquid interface. (
  • The methods followed by SAGE données à l'appui des recommandations sont publiés en même and the processes for preparation of vaccine position temps que la note de synthèse. (
  • These include vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), stromal cell-derived factor 1 (SDF-1), fibroblast growth factor (FGF), and interleukin-6 (IL-6). (
  • Biological activity of the overexpressed basic fibroblast growth factor was confirmed in a human umbilical vein endothelial cell proliferation assay. (
  • Marinobufagenin Stimulates Fibroblast Collagen Production and Causes F" by Jihad Elkareh, David J. Kennedy et al. (
  • This was associated with a significant attenuation of both basal and hypoxia/re-oxygenation-induced collagen production by the fibroblasts. (
  • Concentrated fish oil ameliorates non-alcoholic fatty liver disease by regulating fibroblast growth factor 21-adiponectin axis. (
  • The aim of this thesis was to elucidate the cellular and molecular mechanisms by which S. aureus induces it's pathogenic effects on keratinocyte and fibroblast cell lines. (
  • Here we show that fibroblast growth factor 22 (FGF22), a target-derived presynaptic organizer in the mouse hippocampus, induces the expression of insulin-like growth factor 2 (IGF2) for the stabilization of presynaptic terminals. (
  • Based on these findings, we propose that MBG directly induces increases in collagen expression by fibroblasts, and we suggest that this may be important in the cardiac fibrosis seen with experimental renal failure. (
  • CaMKIIδ regulation of IκΒα degradation was explored in adult cardiac fibroblasts exposed to pharmacological stimulation. (
  • After a plasma fibroblast treatment, the targeted skin area will be red and swollen. (
  • Is Plasma Fibroblast treatment safe and does it hurt? (
  • What can I expect after my Plasma Fibroblast treatment? (
  • Several studies have also correlated fibroblast density and cellular alignment with capsular contracture, and more recent reports have shown a functional difference in fibroblasts, with dysregulation of several inflammatory and fibrotic genes noted when comparing fibroblasts derived from non-contracted and contracted breast implant capsules (Kyle and Bayat 2015). (
  • Hydrogen peroxide decreased human skin fibroblast viability with a concurrent increase in ROS generation and cell apoptosis. (
  • Notably, TFPS pre‑treatment reduced oxidative stress and cell apoptosis in hydrogen peroxide‑treated skin fibroblasts. (
  • These results indicated that TFPS alleviated hydrogen peroxide‑induced oxidative stress and apoptosis in skin fibroblasts via upregulation of SIRT1 expression, indicating that TFPS may act as a potential therapeutic agent for oxidative‑stress‑associated skin diseases and aging. (
  • Farber, John L. / Programmed cell death (apoptosis) of mouse fibroblasts is induced by the topoisomerase II inhibitor etoposide . (
  • Oxidative stress has been suggested as a mechanism underlying skin aging, as it triggers apoptosis in various cell types, including fibroblasts, which play important roles in the preservation of healthy, youthful skin. (
  • In this study, we investigated the effect of (+)-catechin on apoptosis induced by oxidative stress in fibroblasts. (
  • The effect of (+)-catechin on cell viability, apoptosis, phosphorylation of c-Jun terminal kinases (JNK) and p38, and activation of caspase-3 in fibroblasts under oxidative stress were evaluated. (
  • Pretreatment of the fibroblasts with (+)-catechin inhibited hydrogen peroxide-induced apoptosis and reduced phosphorylation of JNK and p38 and activation of caspase-3. (
  • it reduces the number of skin fibroblasts by inducing apoptosis and decreasing their regenerative capacity, which in turn leads to increased skin sagging. (
  • Therefore, suppression of oxidative stress-induced apoptosis in skin fibroblasts is a potential treatment and prevention strategy for maintaining healthy youthful skin. (
  • In this study, we demonstrate that (+)-catechin has an inhibitory effect against oxidative stress-induced apoptosis in fibroblasts, accompanied by suppression of phosphorylation of p38 and c-Jun terminal kinases (JNK), both of which play an important role in intracellular apoptotic signaling induced by oxidative stress. (
  • The loss of p53 allows the fibroblasts to overcome senescence, enhances expression of CAF effectors, and promotes stromal and cancer cell expansion. (
  • Therefore, we investigated whether pharmacological induction of HO-1 by chronic hemin treatment attenuates senescence and improves dysfunctional mitochondria in COPD fibroblasts. (
  • We conclude that HO-1 attenuates senescence in COPD fibroblasts by protecting, at least in part, against mitochondria dysfunction and restoring mitophagy. (
  • We found the modified flow cytometric method measuring SA-β-gal activity superior in discriminating between degrees of senescence in different populations of fibroblasts. (
  • The aim of the present study was to investigate the protective effects of TFPS on hydrogen peroxide‑induced injury of human skin fibroblasts and to elucidate the aforementioned underlying mechanisms. (
  • A hydrogen peroxide‑induced human skin fibroblast injury model was firstly established. (
  • Here we describe an optimized protocol to generate iAstrocytes starting from skin fibroblasts and this approach can be adapted for a wide range of somatic cell types. (
  • Treatment of fibroblasts with glyoxal reproduces some of the modifications found in the skin of diabetics. (
  • A previous isolation of a human cytomegalovirus (CMV) from fibroblasts derived from intact skin of a Charcot-Marie-Tooth disease patient has prompted examination of six blind-coded cultured human skin lines by CMV DNA hybridization. (
  • 1 reported that primary cultures of human skin fibroblasts "possess muscarinic receptors with the properties of specific binding, saturability, pharmacologic specificity, inhibition of norepinephrine-stimulated adenylate cyclase, and increased binding after incubation with an antagonist. (
  • abstract = "To the Editor: In the July 26 issue, Nadi et al.1 reported that primary cultures of human skin fibroblasts "possess muscarinic receptors with the properties of specific binding, saturability, pharmacologic specificity, inhibition of norepinephrine-stimulated adenylate cyclase, and increased binding after incubation with an antagonist. (
  • Skin fibroblasts were isolated from young (mean age ± SD: 25.5 ± 1.8) and very old (age 90.2 ± 0.3) subjects. (
  • Our regular price is 240.00 usd for … Fibroblast or plasma skin resurfacing is a collagen induction therapy. (
  • Fibroblast is the world's most advanced, non-invasive, skin lifting, skin tightening and rejuvenation device used to treat wrinkles, and sagging, dull skin. (
  • The Fibroblast Pen is an FDA approved, CE approved device used to perform fibroblast therapy, a technique used to stimulate production of collagen in the skin. (
  • Ett alternativ till kirurgiskt ingrepp med effektivt Upper lower eye-lid lift before and after 10 days #Plasmapenresults by Dr. Mir Plasma Pen Fibroblast Technology is a great option for: Skin Tightening Med hjälp av plasmaljus stramas huden åt på ögonlocken. (
  • Detta en helt ny behandling för uppstramning eller "​Skin tightening" med plasmaimpulser. (
  • The Fibroblast treatment uses a Plasma Pen to remove excess skin from the eyelid which causes hooding. (
  • Fibroblast Skin Tightening. (
  • These treatments are slightly technical, but the Milwaukee Specialists are … The Plasma Pen/Fibroblast Pen Skin Tightening treatment is an effective anti-aging solution for those who do not want to undergo invasive, surgical cosmetic procedures. (
  • Treatments are Non Invasive Beauty treatments carried out by Certified Fibroblast Skin tightening Technicians and Certified Body Contouring Technicians. (
  • PlasmaPen treats the fibroblasts in your skin more profoundly than a laser or a chemical peel can, leaving the top layer of your skin free of unsightly wounds. (
  • Plasma fibroblast is a non-surgical cosmetic procedure designed to improve the appearance of skin. (
  • The skin rejuvenation results from plasma fibroblast rival those normally only achieved with invasive surgery. (
  • While Plasma fibroblast Skin Tightening therapy is a relatively safe procedure, like with all cosmetic treatments, side effects are possible. (
  • Those who are allergic to topical anesthetics, have a skin infection, or are using isotretinoin shouldn't get plasma fibroblast therapy. (
  • Unfortunately, plasma fibroblast treatments are not as effective on darker skin tones. (
  • Thee most important thing for those thinking about plasma fibroblast therapy is to find a skin clinic that is fully licensed and employs certified technicians who have experience with the technique. (
  • Plasma skin tightening, Fibroblast Plasma, plasma pen, and Fibroblast are some of the names that this treatment goes by. (
  • Plasma Fibroblast skin tightening is associated with success when it comes to tummy tightening, non-surgical eye lifts, and treatment for excess skin on different areas of the body. (
  • What Areas Are Suitable For A Fibroblast Skin Tightening Treatment? (
  • Fibroblast Skin Tightening is proven as a safe and non-invasive technique , provided it is done by a technician that is certified. (
  • As we age, the dermis which is the middle layer of the skin where fibroblasts are found starts to thin which causes it to lose collagen and elastin. (
  • Luckily, fibroblast skin tightening treatments are available to reduce the appearance of fine lines and wrinkles. (
  • How do PlasmaPen fibroblast skin-tightening treatments work? (
  • Fibroblast is the most Advanced Non Invasive Skin Tightening & Lifting for Skin Rejuvenation. (
  • Fibroblast is a non-surgical treatment that uses a device to deliver the energy in the form of plasma to rejuvenate skin by improving facial lines & wrinkles, skin pigmentation associated with aging & other skin irregularities. (
  • Plasma fibroblast is a non-surgical, skin-tightening procedure that rejuvenates the skin and has very little side effects or downtime. (
  • The use of engineered skin equivalents consisting of organotypic cocultures of keratinocytes and fibroblasts offers an attractive approach for such studies. (
  • The expression of integrin α6β4 and of E-cadherin was comparable with that seen in native skin and was not significantly modulated by fibroblasts. (
  • Our Aurorae Pro is a high-quality beauty tool and is the only cosmetic non-laser device that reduces the volume of excess skin by harnessing the power of plasma fibroblast energy. (
  • Keep your skin in tip top shape with the help of our premium plasma fibroblast skin tightening pen. (
  • How will my skin look immediately after the Plasma Fibroblast skin Tightening treatment? (
  • Are there any potential side effects with the Plasma Fibroblast skin Tightening treatment? (
  • Learn about the fibroblast plasma treatment which uses microscopic plasma bubbles for targeted skin tightening. (
  • The main benefit of the Fibroblast Plasma Treatment is that it is a very effective way to achieve skin tightening. (
  • Fibroblast growth factor 21 (FGF21), an atypical member of the FGF family, functions as a hormone that coordinates lipid and glucose metabolism through binding of the FGF receptors in the presence of the coreceptor β -klotho [ 1 - 5 ]. (
  • It appears that the effects of melatonin on lipid metabolism in murine fibroblasts is mediated by melatonin membrane receptors. (
  • Fibroblast growth factor receptors (FGFRs) 1-4 are involved in prostate cancer (PCa) regulation, but the role of FGFR-like 1 (FGFRL1) in PCa is unclear. (
  • The discovery of these 2 potentially novel and unique hernia-associated fibroblasts may lead to the development of novel treatments that can nonsurgically prevent or reverse inguinal hernias. (
  • Treatments provided by Premier Fibroblast Skintight are not medical treatments, nor use of medical grade equipment. (
  • Are you wondering exactly how fibroblast treatments could help your clients? (
  • Enabled fibroblast-like synoviocytes (FLSs) plays a central role in synovial pannus formation and joint destruction in rheumatoid arthritis (RA). (
  • Fibroblast like synoviocytes (FLSs) exert important effects in the occurrence and progression of rheumatoid arthritis (RA). (
  • The aim of this study was to elucidate the role of fibroblasts in bisphosphonate-related osteonecrosis of the jaw (BRONJ), evaluating the effect of zoledronate, alendronate, and ibandronate on the proliferation of fibroblasts and on their expression of genes essential for fibroblast physiology. (
  • With increasing numbers of fibroblasts incorporated into the collagen matrix, the expression of markers associated with keratinocyte activation, e.g. keratins 6, 16 and 17 and the cornified envelope precursor SKALP decreased, and involucrin localization shifted toward the granulosum layer. (
  • Conclusions: Our results illustrate that numbers of fibroblasts in the collagen matrix and their functional state is a critical factor for establishment of normal epidermal morphogenesis. (
  • Reactive oxygen species (ROS) also promote the conversion of fibroblasts into highly migrating myofibroblasts via the accumulation of the hypoxia-inducible factor-1α (HIF-1α) transcription factor and the CXCL12 chemokine. (
  • Fig. 1: Conversion of fibroblasts and the molecular characterization of CiPCs. (
  • These cytokines may influence the present fibroblasts, the key effectors in the defective ECM repair and maintenance in COPD. (
  • Previous studies have shown that fibroblast growth factor 23 (FGF23), which has an important role in phosphate metabolism, is elevated in HD patients. (
  • The current study was undertaken to uncover the role of melatonin in lipid metabolism in the murine fibroblasts. (
  • However, low levels of melatonin, with or without oleic acid, did not influence lipid metabolism in the cultured fibroblasts. (
  • Fibroblast growth factor 23 (FGF-23) is a phosphate metabolism regulator in patients with chronic kidney disease (CKD). (
  • Phosphoribosylpyrophosphate and purine metabolism in cultured fibroblasts. (
  • Studies on the metabolism and biological effects of nitropyrene and related nitro-polycyclic aromatic compounds in diploid human fibroblasts / Veronica M. Maher, Joe Dale Patton, and J. Justin McCormick. (
  • Primary adult rat cardiac fibroblasts were then used to study the effects of selective CaMKII inhibition on pharmacologically-induced NF-κB activation as well as interaction between CaMKII and specific IKK isoforms in a cardiac cellular setting. (
  • Eliminate fine lines, wrinkles, and more with Fibroblast. (
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  • Plasma fibroblast can also treat wrinkles on the face or neck, improve the appearance of scarring- including stretch marks, acne, and surgical scars. (
  • Use the fibroblast plasma pen to remove wrinkles from your eyelids, eyebags, and even your forehead. (
  • The effects of different doses of beta radiation from a strontium-90 source on the proliferation of human Tenon's capsule fibroblasts were studied. (
  • beta Radiation reduces the proliferation of human Tenon's capsule fibroblasts, and at higher doses this effect may be more pronounced one and two weeks after irradiation. (
  • We elucidated the developmental origins of cardiac fibroblasts and characterized their corresponding phenotypes. (