Fibroblasts
Fibroblast Growth Factor 2
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
Fibroblast Growth Factors
A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.
Cells, Cultured
Receptors, Fibroblast Growth Factor
Specific molecular sites or structures on cell membranes that react with FIBROBLAST GROWTH FACTORS (both the basic and acidic forms), their analogs, or their antagonists to elicit or to inhibit the specific response of the cell to these factors. These receptors frequently possess tyrosine kinase activity.
Fibroblast Growth Factor 1
A 17-kDa single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. It binds to HEPARIN, which potentiates its biological activity and protects it from proteolysis. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages, and also has chemotactic and mitogenic activities. It was originally named acidic fibroblast growth factor based upon its chemical properties and to distinguish it from basic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 2).
Skin
Receptor, Fibroblast Growth Factor, Type 1
A fibroblast growth factor receptor with specificity for FIBROBLAST GROWTH FACTORS; HEPARAN SULFATE PROTEOGLYCAN; and NEURONAL CELL ADHESION MOLECULES. Several variants of the receptor exist due to multiple ALTERNATIVE SPLICING of its mRNA. Fibroblast growth factor receptor 1 is a tyrosine kinase that transmits signals through the MAP KINASE SIGNALING SYSTEM.
Receptor, Fibroblast Growth Factor, Type 2
A fibroblast growth factor receptor that is found in two isoforms. One receptor isoform is found in the MESENCHYME and is activated by FIBROBLAST GROWTH FACTOR 2. A second isoform of fibroblast growth factor receptor 2 is found mainly in EPITHELIAL CELLS and is activated by FIBROBLAST GROWTH FACTOR 7 and FIBROBLAST GROWTH FACTOR 10. Mutation of the gene for fibroblast growth factor receptor 2 can result in craniosynostotic syndromes (e.g., APERT SYNDROME; and CROUZON SYNDROME).
Cell Division
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Collagen
3T3 Cells
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Receptor, Fibroblast Growth Factor, Type 3
Dermis
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Gene Expression Regulation
Transfection
Cell Transformation, Neoplastic
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Keloid
A sharply elevated, irregularly shaped, progressively enlarging scar resulting from formation of excessive amounts of collagen in the dermis during connective tissue repair. It is differentiated from a hypertrophic scar (CICATRIX, HYPERTROPHIC) in that the former does not spread to surrounding tissues.
Fibroblast Growth Factor 10
Extracellular Matrix
Fibroblast Growth Factor 7
Collagen Type I
Growth Substances
Base Sequence
Chick Embryo
Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
NIH 3T3 Cells
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)
Cell Aging
Fibronectins
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
Receptor, Fibroblast Growth Factor, Type 4
Cell Differentiation
Embryo, Mammalian
Lung
Cell Line, Transformed
Cell Movement
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Gene Expression
Fibrosis
Phosphorylation
Platelet-Derived Growth Factor
Fibroblast Growth Factor 5
Blotting, Western
Synovial Membrane
Scleroderma, Systemic
A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA.
Fibroblast Growth Factor 4
Cell Survival
Coculture Techniques
Transforming Growth Factor beta1
A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.
Culture Media, Conditioned
Mutation
Reverse Transcriptase Polymerase Chain Reaction
Matrix Metalloproteinase 1
Amino Acid Sequence
Transcription, Genetic
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Phenotype
Up-Regulation
Cell Transformation, Viral
Enzyme Activation
Immunohistochemistry
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Connective Tissue
Dose-Response Relationship, Drug
Interleukin-1
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Procollagen
Pulmonary Fibrosis
Cell Communication
Fibroblast Growth Factor 8
Stromal Cells
Blotting, Northern
Cell Cycle
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
DNA-Binding Proteins
Cell Transdifferentiation
Cricetinae
Mitogens
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Tumor Cells, Cultured
Protein Binding
Cell Membrane
Promoter Regions, Genetic
Receptors, Cell Surface
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Microscopy, Fluorescence
Membrane Proteins
Epidermal Growth Factor
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Epithelial Cells
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
L Cells (Cell Line)
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Paracrine Communication
Extracellular Matrix Proteins
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Microbial Collagenase
A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Down-Regulation
Ultraviolet Rays
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Mucolipidoses
A group of inherited metabolic diseases characterized by the accumulation of excessive amounts of acid mucopolysaccharides, sphingolipids, and/or glycolipids in visceral and mesenchymal cells. Abnormal amounts of sphingolipids or glycolipids are present in neural tissue. INTELLECTUAL DISABILITY and skeletal changes, most notably dysostosis multiplex, occur frequently. (From Joynt, Clinical Neurology, 1992, Ch56, pp36-7)
Transcription Factors
Receptor Protein-Tyrosine Kinases
Protein-Tyrosine Kinases
Proto-Oncogene Proteins
Connective Tissue Growth Factor
Keratinocytes
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
Skin Physiological Phenomena
Lysosomes
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Carrier Proteins
Cattle
Culture Media, Serum-Free
Connective Tissue Cells
Gels
Intercellular Signaling Peptides and Proteins
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
Cornea
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Enzyme Inhibitors
Protein-Serine-Threonine Kinases
DNA Primers
Glycosaminoglycans
Immunoblotting
Models, Biological
Cytoskeleton
Dinoprostone
Cloning, Molecular
Cycloheximide
Tumor Suppressor Protein p53
Myocardium
Heparin
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Nuclear Proteins
Hyaluronic Acid
Diploidy
Electrophoresis, Polyacrylamide Gel
Mitogen-Activated Protein Kinases
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
Tumor Necrosis Factor-alpha
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Avian Sarcoma Viruses
Fetus
Heparitin Sulfate
Genes, ras
Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.
Matrix Metalloproteinase 3
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
DNA, Complementary
Recombinant Fusion Proteins
Rabbits
Flow Cytometry
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Bleomycin
Cell Count
Collagen Type III
Tetradecanoylphorbol Acetate
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Oncogenes
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
Binding Sites
DNA Damage
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
Protein Biosynthesis
Gene Expression Regulation, Enzymologic
Smad3 Protein
Gelatinases
Retroviridae
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Fibroblast Growth Factor 3
Arthritis, Rheumatoid
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
Proto-Oncogene Proteins c-sis
Hybrid Cells
Interleukin-6
Periodontal Ligament
Culture Techniques
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Vascular Endothelial Growth Factor A
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
Rats, Sprague-Dawley
Antibodies
Swiss 3T3 Cells
Glycoproteins
Cytokines
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Simian virus 40
Gene Expression Profiling
Mice, Transgenic
Neovascularization, Physiologic
Mitogen-Activated Protein Kinase 3
Genes, src
Retrovirus-associated DNA sequences (src) originally isolated from the Rous sarcoma virus (RSV). The proto-oncogene src (c-src) codes for a protein that is a member of the tyrosine kinase family and was the first proto-oncogene identified in the human genome. The human c-src gene is located at 20q12-13 on the long arm of chromosome 20.
Precipitin Tests
Matrix Metalloproteinase 2
Granulation Tissue
Cytomegalovirus
A genus of the family HERPESVIRIDAE, subfamily BETAHERPESVIRINAE, infecting the salivary glands, liver, spleen, lungs, eyes, and other organs, in which they produce characteristically enlarged cells with intranuclear inclusions. Infection with Cytomegalovirus is also seen as an opportunistic infection in AIDS.
Glycopeptides from the surgace of human neuroblastoma cells. (1/31038)
Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with Pronase, and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells, and a fibroblast line derived from neuroblastoma tumor tissue show less complex glycopeptides. Although some differences exist between the cell lines and the primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking. (+info)Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (2/31038)
Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s). (+info)Lymphocyte proliferation inhibitory factor (PIF) in alcoholic liver disease. (3/31038)
Lymphocyte proliferation inhibitory factor (PIF) was determined in the supernatants of PHA-stimulated lymphocytes from patients with alcoholic liver disease. PIF was assayed by determining inhibition of DNA synthesis in WI-38 human lung fibroblasts. A two-fold greater inhibition in thymidine incorporation into DNA by lung fibroblasts was observed in supernatants of PHA stimulated lymphocytes from patients with alcoholic hepatitis or active Laennec's cirrhosis as compared with that found in control subjects or patients with fatty liver. It is suggested that decreased liver cell regeneration seen in some patients with alcoholic hepatitis may be due to increased elaboration of PIF. (+info)CAR-dependent and CAR-independent pathways of adenovirus vector-mediated gene transfer and expression in human fibroblasts. (4/31038)
Primary fibroblasts are not efficiently transduced by subgroup C adenovirus (Ad) vectors because they express low levels of the high-affinity Coxsackie virus and adenovirus receptor (CAR). In the present study, we have used primary human dermal fibroblasts as a model to explore strategies by which Ad vectors can be designed to enter cells deficient in CAR. Using an Ad vector expressing the human CAR cDNA (AdCAR) at high multiplicity of infection, primary fibroblasts were converted from being CAR deficient to CAR sufficient. Efficiency of subsequent gene transfer by standard Ad5-based vectors and Ad5-based vectors with alterations in penton and fiber was evaluated. Marked enhancement of binding and transgene expression by standard Ad5 vectors was achieved in CAR-sufficient fibroblasts. Expression by AdDeltaRGDbetagal, an Ad5-based vector lacking the arginine-glycine-aspartate (RGD) alphaV integrin recognition site from its penton base, was achieved in CAR-sufficient, but not CAR-deficient, cells. Fiber-altered Ad5-based vectors, including (a) AdF(pK7)betagal (bearing seven lysines on the end of fiber) (b) AdF(RGD)betagal (bearing a high-affinity RGD sequence on the end of fiber), and (c) AdF9sK betagal (bearing a short fiber and Ad9 knob), demonstrated enhanced gene transfer in CAR-deficient fibroblasts, with no further enhancement in CAR-sufficient fibroblasts. Together, these observations demonstrate that CAR deficiency on Ad targets can be circumvented either by supplying CAR or by modifying the Ad fiber to bind to other cell-surface receptors. (+info)Concomitant activation of pathways downstream of Grb2 and PI 3-kinase is required for MET-mediated metastasis. (5/31038)
The Met tyrosine kinase - the HGF receptor - induces cell transformation and metastasis when constitutively activated. Met signaling is mediated by phosphorylation of two carboxy-terminal tyrosines which act as docking sites for a number of SH2-containing molecules. These include Grb2 and p85 which couple the receptor, respectively, with Ras and PI 3-kinase. We previously showed that a Met mutant designed to obtain preferential coupling with Grb2 (Met2xGrb2) is permissive for motility, increases transformation, but - surprisingly - is impaired in causing invasion and metastasis. In this work we used Met mutants optimized for binding either p85 alone (Met2xPI3K) or p85 and Grb2 (MetPI3K/Grb2) to evaluate the relative importance of Ras and PI 3-kinase as downstream effectors of Met. Met2xPI3K was competent in eliciting motility, but not transformation, invasion, or metastasis. Conversely, MetP13K/Grb2 induced motility, transformation, invasion and metastasis as efficiently as wild type Met. Furthermore, the expression of constitutively active PI 3-kinase in cells transformed by the Met2xGrb2 mutant, fully rescued their ability to invade and metastasize. These data point to a central role for PI 3-kinase in Met-mediated invasiveness, and indicate that simultaneous activation of Ras and PI 3-kinase is required to unleash the Met metastatic potential. (+info)Differential stability of the DNA-activated protein kinase catalytic subunit mRNA in human glioma cells. (6/31038)
DNA-dependent protein kinase (DNA-PK) functions in double-strand break repair and immunoglobulin [V(D)J] recombination. We previously established a radiation-sensitive human cell line, M059J, derived from a malignant glioma, which lacks the catalytic subunit (DNA-PKcs) of the DNA-PK multiprotein complex. Although previous Northern blot analysis failed to detect the DNA-PKcs transcript in these cells, we show here through quantitative studies that the transcript is present, albeit at greatly reduced (approximately 20x) levels. Sequencing revealed no genetic alteration in either the promoter region, the kinase domain, or the 3' untranslated region of the DNA-PKcs gene to account for the reduced transcript levels. Nuclear run-on transcription assays indicated that the rate of DNA-PKcs transcription in M059J and DNA-PKcs proficient cell lines was similar, but the stability of the DNA-PKcs message in the M059J cell line was drastically (approximately 20x) reduced. Furthermore, M059J cells lack an alternately spliced DNA-PKcs transcript that accounts for a minor (5-20%) proportion of the DNA-PKcs message in all other cell lines tested. Thus, alterations in DNA-PKcs mRNA stability and/or the lack of the alternate mRNA may result in the loss of DNA-PKcs activity. This finding has important implications as DNA-PKcs activity is essential to cells repairing damage induced by radiation or radiomimetric agents. (+info)Signals from the Ras, Rac, and Rho GTPases converge on the Pak protein kinase in Rat-1 fibroblasts. (7/31038)
Ras plays a key role in regulating cellular proliferation, differentiation, and transformation. Raf is the major effector of Ras in the Ras > Raf > Mek > extracellular signal-activated kinase (ERK) cascade. A second effector is phosphoinositide 3-OH kinase (PI 3-kinase), which, in turn, activates the small G protein Rac. Rac also has multiple effectors, one of which is the serine threonine kinase Pak (p65(Pak)). Here we show that Ras, but not Raf, activates Pak1 in cotransfection assays of Rat-1 cells but not NIH 3T3 cells. We tested agents that activate or block specific components downstream of Ras and demonstrate a Ras > PI 3-kinase > Rac/Cdc42 > Pak signal. Although these studies suggest that the signal from Ras through PI 3-kinase is sufficient to activate Pak, additional studies suggested that other effectors contribute to Pak activation. RasV12S35 and RasV12G37, two effector mutant proteins which fail to activate PI 3-kinase, did not activate Pak when tested alone but activated Pak when they were cotransfected. Similarly, RacV12H40, an effector mutant that does not bind Pak, and Rho both cooperated with Raf to activate Pak. A dominant negative Rho mutant also inhibited Ras activation of Pak. All combinations of Rac/Raf and Ras/Raf and Rho/Raf effector mutants that transform cells cooperatively stimulated ERK. Cooperation was Pak dependent, since all combinations were inhibited by kinase-deficient Pak mutants in both transformation assays and ERK activation assays. These data suggest that other Ras effectors can collaborate with PI 3-kinase and with each other to activate Pak. Furthermore, the strong correlation between Pak activation and cooperative transformation suggests that Pak activation is necessary, although not sufficient, for cooperative transformation of Rat-1 fibroblasts by Ras, Rac, and Rho. (+info)Differential roles for cyclin-dependent kinase inhibitors p21 and p16 in the mechanisms of senescence and differentiation in human fibroblasts. (8/31038)
The irreversible G1 arrest in senescent human diploid fibroblasts is probably caused by inactivation of the G1 cyclin-cyclin-dependent kinase (Cdk) complexes responsible for phosphorylation of the retinoblastoma protein (pRb). We show that the Cdk inhibitor p21(Sdi1,Cip1,Waf1), which accumulates progressively in aging cells, binds to and inactivates all cyclin E-Cdk2 complexes in senescent cells, whereas in young cells only p21-free Cdk2 complexes are active. Furthermore, the senescent-cell-cycle arrest occurs prior to the accumulation of the Cdk4-Cdk6 inhibitor p16(Ink4a), suggesting that p21 may be sufficient for this event. Accordingly, cyclin D1-associated phosphorylation of pRb at Ser-780 is lacking even in newly senescent fibroblasts that have a low amount of p16. Instead, the cyclin D1-Cdk4 and cyclin D1-Cdk6 complexes in these cells are associated with an increased amount of p21, suggesting that p21 may be responsible for inactivation of both cyclin E- and cyclin D1-associated kinase activity at the early stage of senescence. Moreover, even in the late stage of senescence when p16 is high, cyclin D1-Cdk4 complexes are persistent, albeit reduced by +info)E-GEOD-13681 - Genome wide analysis of gene expression of rat ES cells, rat embryonic fibroblast cells and mouse ES cells -...
Primary Human Cardiac Fibroblast Cells best suppliers/vendors - Labshake
Retinoic acid restores normal growth control to a transformed mouse embryo fibroblast cell line<...
Tumor immunotherapy targeting fibroblast activation protein, a product expressed in tumor-associated fibroblasts | Read by QxMD
Dexamethasone and Salbutamol Stimulate Human Lung Fibroblast Proliferation | World Allergy Organization Journal | Full Text
Genetic characterization of skin fibroblast cell lines by DNA fingerprinting Archives » Anandpub
Genetic characterization of skin fibroblast cell lines by DNA fingerprinting » Anandpub
PFG acted as an inducer of premature senescence in TIG-1 normal diploid fibroblast and an inhibitor of mitosis in the HeLa...
Gene expression profiling of cholangiocarcinoma-derived fibroblast reveals alterations related to tumor progression and...
Abstract 953: Ca2+-permeable TRP Channels In Human Cardiac Fibroblasts | Circulation
Protective effect of resveratrol against caspase 3 activation in primary mouse fibroblasts<...
Altered transcriptome signature of phenotypically normal skin fibroblasts heterozygous for CDKN2A in familial melanoma:...
A distinct de novo expression of Nav1.5 sodium channels in human atrial fibroblasts differentiated into myofibroblasts. |...
Human Fibroblasts
Effects of ebselen and N-acetyl cysteine on replicative aging of primary human fibroblast strains | Immunity & Ageing | Full...
The connexin mimetic peptide Gap27 increases human dermal fibroblast migration in hyperglycemic and hyperinsulinemic conditions...
Human fibroblasts can express and transport both PC-I a | Open-i
Shear Stress Modulates Gene Expression in Normal Human Dermal Fibroblasts
RE: [Histonet] fibroblast marker
Prostaglandin E2 induces Skin Aging via E-prostanoid 1 in Normal Human Dermal Fibroblasts[v1] | Preprints
The inhibition of human lung fibroblast proliferation and differentiation by Gs-coupled receptors is not predicted by the...
Tumor Cell Invasion Is Promoted by Interstitial Flow-Induced Matrix Priming by Stromal Fibroblasts | Cancer Research
c-Src enhances the spreading of src-/-fibroblasts on fibronectin by a kinase-independent mechanism<...
Fibroblast-derived MT1-MMP promotes tumor progression in vitro and in vivo | BMC Cancer | Full Text
Fibroblast-derived MT1-MMP promotes tumor progression in vitro and in vivo | BMC Cancer | Full Text
Regulation of fibroblast Fas expression by soluble and mechanical pro-fibrotic stimuli | Respiratory Research | Full Text
Bustle behind the scenes of action : nemosis as a model for fibroblast inflammatory activation
Human Pulmonary Fibroblast Cell Pellet - Science PRO
Abstract 250: Elucidating the Role of Platelet Derived Growth Factor Receptor Alpha Signaling in Cardiac Fibroblasts |...
Cross-Species Functional Analysis of Cancer-Associated Fibroblasts Identifies a Critical Role for CLCF1 and IL-6 in Non-Small...
The methyltransferase SET9 regulates TGF B-1 activation of renal fibroblasts via interaction with SMAD3 | Journal of Cell...
Identification and characterization of a fibroblast marker: FSP1. | JCB
Human Pulmonary Fibroblast MicroRNA - Science PRO
The transcription factor scleraxis is a critical regulator of cardiac fibroblast phenotype | BMC Biology | Full Text
Expression of the BIRC5 gene in the presence of adalimumab in normal human dermal fibroblasts (NHDF) - World Scientific News -...
RUNG - The effect of serum withdrawal on the protein profile of quiescent human dermal fibroblasts in primary cell culture.
SAT0318 Epigenetic regulation of FRA2 by JMJD3 regulates fibroblast activation in systemic sclerosis | Annals of the Rheumatic...
Aging | Induction of fibroblast senescence generates a non-fibrogenic myofibroblast phenotype that differentially impacts on...
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3T3 cells - Wikipedia
Foetal-to-adult transitions in fibroblast phenotype: their possible relevance to the pathogenesis of cancer<...
RhoA knockout fibroblasts lose tumor-inhibitory capacity in vitro and promote tumor growth in vivo
Abstract 20971: Differential Electrophysiological Properties of Atrial Fibroblasts From AF versus Sinus Rhythm Patients |...
JCI -
Dysfunction of the MDM2/p53 axis is linked to premature aging
Marked TGF-β-regulated miRNA expression changes in both COPD and control lung fibroblasts - Research database - University of...
Human Skin Cells (Dermal Fibroblasts) (DMD) [ASE-5014] - $924.00 : AppliedStemCell eCommerce Platform, Advance stem cell...
Human Skin Cells (Dermal Fibroblasts) (LCP) [ASE-5055] - $924.00 : AppliedStemCell eCommerce Platform, Advance stem cell...
Asian Science Citation Index - Articles written by S. I. Rennard
Role of periodontal ligament fibroblasts in osteoclastogenesis: a review
The effect of carboxymethyl-chitosan on proliferation and collagen secretion of normal and keloid skin fibroblasts<...
The expression of proliferation-dependent antigens during the lifespan of normal and progeroid human fibroblasts in culture |...
The Comparison of the Levels of Leukotriene B4, TGF-β1, Collagen in Keloid Fibroblasts and Normal Skin Fibroblast
JAIRO | Proteoglycan from salmon nasal cartilage promotes in vitro wound healing of fibroblast monolayers via the CD44 receptor
Molecules | Free Full-Text | Inhibitory Effects of Enalaprilat on Rat Cardiac Fibroblast Proliferation via ROS/P38MAPK/TGF-β1...
Influence of E-smoking liquids on human periodontal ligament fibroblasts | Head & Face Medicine | Full Text
Early responses of human periodontal ligament fibroblasts to cyclic and static mechanical stretching - Zurich Open Repository...
Differential binding of <sup>125</sup>I-IGF-I preparations to human fibroblast...
Lysocardiolipin acyltransferase regulates TGF-β mediated lung fibroblast differentiation<...
Effects of (1→3), (1→6)-β-D-glucan behavior in human dermal fibroblast cells under serum starvation<...
Opposing effects of protein kinase Calpha and protein kinase Cepsilon on collagen expression by human lung fibroblasts are...
Resident fibroblasts in the kidney: a major driver of fibrosis and inflammation | Inflammation and Regeneration | Full Text
synovial fibroblast osteoarthritis
Lipoteichoic acid enhances IL-6 production in human synovial fibroblasts via TLR2 receptor, PKCdelta and c-Src dependent...
Modulation of fibroblast morphology and adhesion during collagen matrix remodeling<...
WISP1, a pro-mitogenic, pro-survival factor, mediates tumor necrosis factor-α (TNF-α)-stimulated cardiac fibroblast...
Leukoregulin down-regulates type I collagen mRNA levels and promoter activity in human dermal fibroblasts, and counteracts the...
Metabolomics Studies Show Dose-Dependent Toxicity Induced by SiO2 Nanoparticles in MRC-5 Human Fetal Lung Fibroblasts - Huang -...
Cynomolgus Monkey Primary Cardiac Fibroblasts
Clinical variation in X-linked adrenoleukodystrophy: Fatty acid and lipid metabolism in cultured fibroblasts<...
北京大学医学部机构知识库([email protected]): Sphingosylphosphorylcholine induces alpha-smooth muscle actin expression in human lung fibroblasts and...
Collagen binding proteins derived from the embryonic fibroblast cell surface recognize arginine-glycine-aspartic acid |...
Human Synovial fibroblasts - Osteoarthritis
Human Synovial fibroblasts
Effects of THz radiation on human fibroblasts in-vitro: Exposure set-up and biological endpoints</em>...
Resolution of human fibroblast interferon into 2 distinct classes by thiol exchange chromatography - GeoScience.net
Regulation of fibroblast migration by tenascin-C. - Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal...
The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy | BUT...
Marked reduction of alcohol dehydrogenase in keratoconus corneal fibroblasts<...
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Primary Dermal Fibroblast; Normal, Human, Adult (HDFa) ATCC ® PCS
Effects of physiological electric fields on migration of human dermal fibroblasts -ORCA
Fibroblasts invade at a snails pace
Heat Shock Protein 90 Inhibitor (17-AAG) Induces Apoptosis and Decreases Cell Migration/Motility of Keloid Fibroblasts<...
MicroRNA expression analysis of human skin fibroblasts treated with high-fluence light-emitting diode-red light<...
Significant improvement of direct reprogramming efficacy of fibroblasts into progenitor endothelial cells by ETV2 and hypoxia |...
Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation<...
Skin fibroblast identity | mysite
Tumor cell invasion is promoted by interstitial flow-induced matrix priming by stromal fibroblasts - Infoscience
Plasma Fibroblast Therapy - What You Need to Know | Vertical Dynamics
Low-dose of ionizing radiation enhances cell proliferation via transient ERK1/2 and p38 activation in normal human lung...
Survival of encapsulated human primary fibroblasts and erythropoietin expression under xenogeneic conditions - Infoscience
Expression of hydrogen peroxide and glutathione metabolizing enzymes in human skin fibroblasts derived from donors of different...
IL-13 induces YY1 through the AKT pathway in lung fibroblasts
c-Src Mediates Early Human Corneal Fibroblast Responses To Ad19 Infection | IOVS | ARVO Journals
A cell cycle study of the effects of Con A on synchronized mouse embryo fibroblasts: Arrest and dissociation between uptake of...
Frontiers | Alteration of the antitumor immune response by cancer-associated fibroblasts | Immunology
Myxedema
"Fibroblast". Genetics Home Reference. U.S. National Library of Medicine. 2014-05-05. Retrieved 2014-05-10. "Fibroblasts". ... It is thought that fibroblast stimulation by the thyroid stimulating hormone (TSH) receptor increases the deposition of ... The increased deposition of glycosaminoglycan is not fully understood, however, two mechanisms predominate: Fibroblast ...
Azficel-T
It consists of fibroblasts harvested from the patient's own skin. It was approved for medical use in the United States in June ... "Autologous Fibroblasts". U.S. Food and Drug Administration (FDA). 29 June 2017. Archived from the original on 22 July 2017. ... July 2012). "A multicenter, double-blind, placebo-controlled trial of autologous fibroblast therapy for the treatment of ...
Desmoplasia
In the reactive stroma hypothesis, tumor cells cause the proliferation of fibroblasts and subsequent secretion of collagen. The ... Myofibroblastic cells in tumors are differentiated from fibroblasts for their positive staining of smooth-muscle actin (SMA). ... These scars are hypercellular with fibroblasts, myofibroblasts, and some immune cells present. The immature scars can be ... The tumor-induced stromal change hypothesis claims that tumor cells can dedifferentiate into fibroblasts and, themselves, ...
Prostaglandin EP3 receptor
... mouth gingival fibroblasts; and eye (corneal endothelium and keratocytes, trabecular cells, ciliary epithelium, and ...
Michael Abercrombie
Monolayering' of fibroblasts. Exp. Cell Res. 6: 293-306. Abercrombie is also known for editing and co-founding the Penguin New ... I. Speed of movement of chick heart fibroblasts in relation to their mutual contacts. Exp. Cell Res. 5: 111-131. Abercrombie, M ...
Prostaglandin EP4 receptor
... and basic fibroblast growth factor-induced osteoclast formation; bone taken from EP4(-/-) mice to re-absorb bone when induced ... and gingival fibroblasts. Standard prostanoids have the following relative efficacies in binding to and activating EP4: PGE2> ... mRNA through activation of p38 mitogen-activated protein kinase in interleukin-1 beta-treated human synovial fibroblasts". The ...
15-Hydroxyeicosatetraenoic acid
... a fibroblast cell line); rat PA adventitia fibroblasts; Baby hamster kidney cells; and diverse types of vascular endothelial ... "Hydroxyeicosatetraenoic acids released through the cytochrome P-450 pathway regulate 3T6 fibroblast growth". The Journal of ...
Stroma of ovary
These appear similar to fibroblasts. The stroma also contains ordinary connective tissue such as reticular fibers and collagen ...
Tunica externa
This is secreted by fibroblasts. The tunica externa provides basic structural support to blood vessels. It prevents vessels ...
Hayflick limit
It has been reported that the limited replicative capability of human fibroblasts observed in cell culture is far greater than ... Hayflick noticed that one of his cultures of embryonic human fibroblasts had developed an unusual appearance and that cell ... Carrel A, Ebeling AH (1921). "Age and multiplication of fibroblasts". J. Exp. Med. 34 (6): 599-606. doi:10.1084/jem.34.6.599. ... The experiment proceeded as follows: Hayflick mixed equal numbers of normal human male fibroblasts that had divided many times ...
Mercury (element)
ISBN 978-0-08-020575-5. Milo G E; Casto B C (1990). Transformation of human diploid fibroblasts. CRC Press. p. 104. ISBN 978-0- ...
Scaling and root planing
Specifically preventing the formation of fibroblasts. An alternate irrigation with povidone-iodine may be used - if no contra- ...
Mark Bretscher
Abercrombie, M; Heaysman, JE; Pegrum, SM (1970). "The locomotion of fibroblasts in culture. I. Movements of the leading edge". ... "Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts". Cell. 10 (3 ... is largely based on how cap formation occurs and the movement of carbon particles on the surfaces of migrating fibroblasts ...
OP9 cell
Mouse Embryonic Fibroblasts (MEF) Gao, J; et al. (2010). "Characterization of OP9 as authentic mesenchymal stem cell line". J ...
Jérôme Lejeune
Technique to culture fibroblasts in vitro]. Revue Française d'Études Cliniques et Biologiques. 5: 406-408. "Past ASHG Award ... In 2009, co-author Gautier claimed that the discovery was based on fibroblast tissue samples that she had prepared and noticed ...
CUL1
It is expressed in lung fibroblasts. The protein is neddylated, which enhances the ubiquitination activity of SCF. Deneddylated ...
Extracellular matrix
Fibroblasts are the most common cell type in connective tissue ECM, in which they synthesize, maintain, and provide a ... Wang JH, Thampatty BP, Lin JS, Im HJ (April 2007). "Mechanoregulation of gene expression in fibroblasts". Gene. 391 (1-2): 1-15 ... Elastins are synthesized by fibroblasts and smooth muscle cells. Elastins are highly insoluble, and tropoelastins are secreted ... Hadjipanayi E, Mudera V, Brown RA (February 2009). "Close dependence of fibroblast proliferation on collagen scaffold matrix ...
Osteonectin
Fibroblasts, including periodontal fibroblasts, synthesize osteonectin. This protein is synthesized by macrophages at sites of ... It disrupts focal adhesions in fibroblasts. It also regulates the proliferation of some cells, especially endothelial cells, ... Osteonectin is expressed by a wide variety of cells, including chondrocytes, fibroblasts, platelets, endothelial cells, ... "An osteonectinlike protein in porcine periodontal ligament and its synthesis by periodontal ligament fibroblasts". Canadian ...
Ovarian cancer
These include fibroblasts and endocrine cells. The symptoms of a sex-cord or stromal ovarian tumor can differ from other types ... and fibroblast growth factor 23. Use of blood test panels may help in diagnosis. The OVA1 panel includes CA-125, beta-2 ...
Bone healing
The fibroblasts within the granulation tissue develop into chondroblasts which also form hyaline cartilage. These two new ... Within this area, the fibroblasts replicate. Within 7-14 days, they form a loose aggregate of cells, interspersed with small ... Other sources of precursor cells are the bone marrow (when present), endosteum, small blood vessels, and fibroblasts. Primary ...
Pretibial myxedema
There is no increase in fibroblasts. Over time, secondary hyperkeratosis may occur, which may become verruciform. Many of these ... The serum contains circulating factors which stimulate fibroblasts to increase synthesis of glycosaminoglycans. There are ...
Mothers against decapentaplegic homolog 4
The FGF (Fibroblast Growth Factor) pathway stimulation leads to Smad4 phosphorylation by Erk of the canonical MAPK site located ... It is also produced by fibroblasts. The functional SMAD 4 participates in the regulation of the TGF-β signal transduction ... "TGF-beta1 stimulates human AT1 receptor expression in lung fibroblasts by cross talk between the Smad, p38 MAPK, JNK, and PI3K ...
Perineurium
The perineurium is composed from fibroblasts. In the peripheral nervous system, the myelin sheath of each axon in a nerve is ...
Tumor microenvironment
Carcinoma associated fibroblasts (CAFs) are a heterogenous group of fibroblasts whose function is pirated by cancer cells and ... fibroblast specific protein 1 (FSP-1) and fibroblast activation protein (FAP). None of these factors can be used to ... As fibroblasts, CAFs are able to rework the ECM to include more paracrine survival signals such as IGF-1 and IGF-2, thus ... As many fibroblasts are transformed into CAFs during carcinogenesis, this reduces the amount of ECM produced and the ECM that ...
Interstitial collagenase
Goldberg GI, Wilhelm SM, Kronberger A, Bauer EA, Grant GA, Eisen AZ (May 1986). "Human fibroblast collagenase. Complete primary ... Interstitial collagenase, also known as fibroblast collagenase, and matrix metalloproteinase-1 (MMP-1) is an enzyme that in ... "1.56 A structure of mature truncated human fibroblast collagenase". Proteins. 19 (2): 98-109. doi:10.1002/prot.340190203. PMID ...
Angiogenesis
The fibroblast growth factor (FGF) family with its prototype members FGF-1 (acidic FGF) and FGF-2 (basic FGF) consists to date ... One of the first applications of pro-angiogenic methods in humans was a German trial using fibroblast growth factor 1 (FGF-1) ... Besides FGF-1, one of the most important functions of fibroblast growth factor-2 (FGF-2 or bFGF) is the promotion of ... Blaber M, DiSalvo J, Thomas KA (February 1996). "X-ray crystal structure of human acidic fibroblast growth factor". ...
Interferon
They are produced by fibroblasts and monocytes. However, the production of type I IFN-α is inhibited by another cytokine known ... Meager A, Graves H, Burke DC, Swallow DM (August 1979). "Involvement of a gene on chromosome 9 in human fibroblast interferon ... Berthold W, Tan C, Tan YH (June 1978). "Chemical modifications of tyrosyl residue(s) and action of human-fibroblast interferon ... Large amounts of human beta interferon were made by superinducing the beta interferon gene in human fibroblast cells. Cantell's ...
DCHS1
The cell-cell adhesion of fibroblasts is thought to be necessary for wound healing. Mutations in this gene have been shown to ... Protein dachsous homolog 1, also known as protocadherin-16 (PCDH16) or cadherin-19 (CDH19) or cadherin-25 (CDH25) or fibroblast ... This particular cadherin family member is expressed in fibroblasts but not in melanocytes or keratinocytes. ... "Multiple cadherins are expressed in human fibroblasts". Biochem. Biophys. Res. Commun. 235 (2): 355-8. doi:10.1006/bbrc. ...
Stress fiber
Kreis, Thomas E.; Birchmeier, Walter (November 1980). "Stress fiber sarcomeres of fibroblasts are contractile". Cell. 22 (2): ... "Identification of Novel Graded Polarity Actin Filament Bundles in Locomoting Heart Fibroblasts: Implications for the Generation ...
MAFK
Embryonic fibroblasts that are derived from Maff−/−::Mafg−/−::Mafk−/− mice fail to activate Nrf2-dependent cytoprotective genes ...
Fibroblast - Wikipedia
Fibroblasts are the most common cells of connective tissue in animals. Fibroblasts have a branched cytoplasm surrounding an ... Fibroblasts, like the tumor-associated host fibroblasts (TAF), play a crucial role in immune regulation through TAF-derived ... The life span of a fibroblast, as measured in chick embryos, is 57 ± 3 days. Fibroblasts and fibrocytes are two states of the ... "Fibroblasts". Retrieved 16 August 2018. Weissman-Shomer P, Fry M (1975). "Chick embryo fibroblasts senscence in vitro: pattern ...
Modelling human blastocysts by reprogramming fibroblasts into iBlastoids | Nature
Human fibroblasts are reprogrammed to generate blastocyst-like structures called iBlastoids, which recapitulate aspects of ... Here we describe the reprogramming of fibroblasts into in vitro three-dimensional models of the human blastocyst, termed ... which propagated in fibroblast medium with classic fibroblast morphology; n = 2. Scale bars, 100 μm (arrows indicate step-wise ... Liu, X., Tan, J.P., Schröder, J. et al. Modelling human blastocysts by reprogramming fibroblasts into iBlastoids. Nature 591, ...
New Technology Directly Reprograms Skin Fibroblasts For a New Role
Researchers have discovered a way to repurpose fibroblasts into functional melanocytes, the bodys pigment-producing cells. The ... New Technology Directly Reprograms Skin Fibroblasts For a New Role. Penn study has implications for new skin disease treatments ... By generating melanocytes from the fibroblasts of melanoma patients, Xu explains, "we can screen not only to find why these ... Newswise - PHILADELPHIA - As the main component of connective tissue in the body, fibroblasts are the most common type of cell ...
P53 and Cancer-Associated Fibroblasts
Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.. *Cancer-Associated Fibroblasts: Their ... Cancer-associated fibroblasts (CAFs) are a major component of the cancer stroma. CAFs enhance the malignant features of nearby ... Resident tissue fibroblasts, bone marrow-derived mesenchymal stem cells, and hematopoietic stem cells are examples of possible ... Origins of cancer-associated fibroblast. Past studies have reported that many different cells could be the origin of CAFs. ...
Direct conversion of fibroblasts to functional neurons by defined factors
Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four ... Direct conversion of fibroblasts to functional neurons by defined factors Nature. 2010 Feb 25;463(7284):1035-41. doi: 10.1038/ ... Recent work has shown that mouse and human fibroblasts can be reprogrammed to a pluripotent state with a combination of four ... that suffice to rapidly and efficiently convert mouse embryonic and postnatal fibroblasts into functional neurons in vitro. ...
Mouse fibroblasts | 1997 Photomicrography Competition | Nikon's Small World
Pharmacologic fibroblast reprogramming into photoreceptors restores vision | Nature
Here we report that the administration of a set of five small molecules can chemically induce the transformation of fibroblasts ... A set of five small molecules can induce the transformation of fibroblasts into rod photoreceptor-like cells, which can ... We show that mitonuclear communication is a key determining factor for the reprogramming of fibroblasts into CiPCs. ... Chemical reprogramming of fibroblasts offers an opportunity to reverse vision loss; however, the generation of sensory neuronal ...
Fibroblast growth factor 21 upregulation | GreenMedInfo
Ascorbic acid attenuates cell stress by activating the fibroblast growth factor 21/fibroblast growth factor receptor 2/ ... 14 Abstracts with Fibroblast growth factor 21 upregulation Research. Filter by Study Type. Animal Study. ... Pharmacological Actions : Fibroblast growth factor 21 upregulation. Additional Keywords : Gene Expression Regulation, Risk ... Fibroblast growth factor-21 could potentially be used as a hormone therapy to extend lifespan in mammals.Dec 31, 2011. ...
StrataGraft (allogeneic cultured keratinocytes and fibroblasts) dosing, indications, interactions, adverse effects, and more
... allogeneic cultured keratinocytes and fibroblasts), frequency-based adverse effects, comprehensive interactions, ... Drugs allogeneic cultured keratinocytes and fibroblasts * 2010gintuit-allogeneic-cultured-keratinocytes-fibroblasts-bovine- ... allogeneic cultured keratinocytes and fibroblasts (Rx). Brand and Other Names:StrataGraft, allogeneic cultured keratinocytes ... encoded search term (allogeneic cultured keratinocytes and fibroblasts (StrataGraft)) and allogeneic cultured keratinocytes and ...
Fibroblast Growth Factor 23 and Left Ventricular Hypertrophy in Hemodialysis Patients
Previous studies have shown that fibroblast growth factor 23 (FGF23), which has an important role in phosphate metabolism, is ... Fibroblast Growth Factor 23 and Left Ventricular Hypertrophy in Hemodialysis Patients () Ayaka Saito1,2, Takako Onuki2, ... Isakova, T. (2013) Fibroblast Growth Factor 23 and Adverse Clinical Outcomes in Chronic Kidney Disease. Current Opinion in ... 2012) Fibroblast Growth Factor 23 and Inflammation in CKD. Clinical Journal of the American Society of Nephrology, 7, 1155-1162 ...
INSIGHTS Fibroblasts Dangerous travel companions - SIAPeC-IAP
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FAQ - Fibroblast Cell Cultures
Induction of pluripotent stem cells from adult human fibroblasts by defined factors
Impact of bisphosphonates on the proliferation and gene expression of human fibroblasts
A role for decorin in controlling proliferation, adhesion, and migration of murine embryonic fibroblasts. J Biomed Mater Res A ... Maddaluno L, Urwyler C, Werner S. Fibroblast growth factors: key players in regeneration and tissue repair. Dev Camb Engl. 2017 ... Extracellular Matrix and Dermal Fibroblast Function in the Healing Wound. Adv Wound Care. 2016;5(3):119-36 ... Zoledronic acid and geranylgeraniol regulate cellular behaviour and angiogenic gene expression in human gingival fibroblasts. J ...
Mechanical regulation of cardiac fibroblast profibrotic phenotypes. | Sigma-Aldrich
Mechanical regulation of cardiac fibroblast profibrotic phenotypes. by Kate M Herum, Jonas Choppe, Aditya Kumar, Adam J Engler ... Mechanical regulation of cardiac fibroblast profibrotic phenotypes.. Molecular biology of the cell. (2017-05-05) ... It occurs as a result of cardiac fibroblast (CFB) activation and differentiation into myofibroblasts, characterized by ... These findings suggest that myocyte stretch, fibroblast stretch, and matrix stiffening following MI may separately regulate ...
Diagnostics | Free Full-Text | Differential Expression Profiles of the Transcriptome and miRNA Interactome in Synovial...
... we performed transcriptome-wide RNA-seq and small RNA-seq on synovial fibroblasts from rheumatoid arthritis (RA) subject and ... next-generation sequencing to decipher the molecular mechanisms underlying aberrant rheumatoid arthritis synovial fibroblasts ( ... Synovial Fibroblasts. Primary human synovial fibroblasts isolated from one normal donor and one RA subject were obtained from ... Pap, T.; Müller-Ladner, U.; Gay, R.E.; Gay, S. Fibroblast biology. Role of synovial fibroblasts in the pathogenesis of ...
CIL:26527, Mus musculus, fibroblast. CIL. Dataset
CIL:13408, Mus musculus, fibroblast. CIL. Dataset
Wildtype mouse embryo fibroblasts were treated with the radiomimetic drug neocarzinostati... ... Wildtype mouse embryo fibroblasts were treated with the radiomimetic drug neocarzinostatin (NCS) for 1hour prior to being fixed ... Jung-Ae Kim, Michael Kruhlak, Farokh Dotiwala, Andre Nussenzweig, James E. Haber (2011) CIL:13408, Mus musculus, fibroblast. ...
CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS<...
CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS. In: Journal of the American Society of Nephrology. 1994 ; ... CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS. Journal of the American Society of Nephrology. 1994;5:496- ... CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS. Claire Loughrey, Paul Jackson, Ann McGinty, Jane McEneny, ... title = "CYTOTOXICITY OF CARBAMYLATED LDL CHOLESTEROL TO HUMAN FIBROBLASTS",. author = "Claire Loughrey and Paul Jackson and ...
Effect of beta radiation on proliferating human Tenon's capsule fibroblasts. | British Journal of Ophthalmology
The cultured fibroblasts were exposed to doses of 100, 250, 500, 750, 1000, 1500, and 3000 rads, and cell numbers were counted ... beta Radiation reduces the proliferation of human Tenons capsule fibroblasts, and at higher doses this effect may be more ... Treatment inhibited the proliferation of the fibroblasts. At seven days the cells exposed to 3000 rads showed a decrease ... of different doses of beta radiation from a strontium-90 source on the proliferation of human Tenons capsule fibroblasts were ...
A Senescence Bystander Effect in Human Lung Fibroblasts - UBC Library Open Collections
A Senescence Bystander Effect in Human Lung Fibroblasts Waters, David W.; Schuliga, Michael; Pathinayake, Prabuddha S.; Wei, ... In this study, we investigated whether senescent human lung fibroblasts (LFs) and alveolar epithelial cells (AECs) could induce ... In this study, we investigated whether senescent human lung fibroblasts (LFs) and alveolar epithelial cells (AECs) could induce ... IPF and cellular senescence is well established and several studies now describe a higher abundance of senescent fibroblasts ...
fibroblasts News Research Tags Articles - Neuroscience News
Neuroscience research articles are provided.. What is neuroscience? Neuroscience is the scientific study of nervous systems. Neuroscience can involve research from many branches of science including those involving neurology, brain science, neurobiology, psychology, computer science, artificial intelligence, statistics, prosthetics, neuroimaging, engineering, medicine, physics, mathematics, pharmacology, electrophysiology, biology, robotics and technology. ...
Fgfr1 MGI Mouse Gene Detail - MGI:95522 - fibroblast growth factor receptor 1
Tremella fuciformis polysaccharide suppresses hydrogen peroxide-triggered injury of human skin fibroblasts via upregulation of...
H2O2-treated skin fibroblasts (n=4, **P,0.01, ***P,0.001). (B) Skin fibroblast apoptosis in control, H2O2-treated (300 µM), 200 ... A) Human skin fibroblasts were cultured in the presence of TFPS (0, 10, 20, 50, 100, 200, and 300 µg/ml), for 24 h, and cell ... However, human skin fibroblasts pretreated with 200 µg/ml TFPS had attenuated H2O2-induced ROS generation, with a decline of ... Human skin fibroblasts (5,000 cells/well) were plated onto 24-well plates, pretreated with TFPS for 1 h and then treated with ...
Assessment of metabolic reprogramming of the skin fibroblasts from patients with mitochondrial diseases using the Seahorse XF...
The mitochondrial function and glycolytic function in MERRF skin fibroblasts were also evaluated by the XF Analyzer as compared ... In this webinar we will discuss our research regarding energy metabolism within the primary cultures of skin fibroblasts from ... Assessment of metabolic reprogramming of the skin fibroblasts from patients with mitochondrial diseases using the Seahorse XF ... Assessment of metabolic reprogramming of the skin fibroblasts from patients with mitochondrial diseases using the Seahorse XF ...
British Library EThOS: Mechanisms by which Staphylococcus aureus induces cytokines and cell death in human keratinocytes and...
mouse fibroblasts ; human keratinocytes ; Staphylococcus aureus ; atopic dermatitis ... NC/Nga mouse fibroblasts that lacked soluble IL-33 (sST2) receptor were more sensitive to the effects of S. aureus than control ... Methods: Human keratinocytes (HEKa), and mouse embryonic fibroblasts (MEF) from the NC/Nga dermatitis prone mouse strain were ... Mechanisms by which Staphylococcus aureus induces cytokines and cell death in human keratinocytes and mouse fibroblasts ...
Identification of Sp1 as a Transcription Activator to Regulate Fibroblast Growth Factor 21 Gene Expression
Fibroblast growth factor 21 (FGF21) is a metabolic hormone with multiple beneficial effects on lipid and glucose homeostasis. ... S. Jiang, C. Yan, Q.-C. Fang et al., "Fibroblast growth factor 21 is regulated by the IRE1α-XBP1 branch of the unfolded protein ... Identification of Sp1 as a Transcription Activator to Regulate Fibroblast Growth Factor 21 Gene Expression. Shuqin Chen. ,1 ... Fibroblast growth factor 21 (FGF21), an atypical member of the FGF family, functions as a hormone that coordinates lipid and ...
Melatonin and lipid uptake by murine fibroblasts: clinical implications. - NeL.edu
The current study was undertaken to uncover the role of melatonin in lipid metabolism in the murine fibroblasts. The results ... However, low levels of melatonin, with or without oleic acid, did not influence lipid metabolism in the cultured fibroblasts. ... It appears that the effects of melatonin on lipid metabolism in murine fibroblasts is mediated by melatonin membrane receptors. ... Maldonado A, Siu N, Sánchez-Hidalgo A, Acuna-Castroviejo A, Escames E, Melatonin and lipid uptake by murine fibroblasts: ...
Functional genomics atlas of synovial fibroblasts defining rheumatoid arthritis heritability | Genome Biology | Full Text
Dermal fibroblastsSynovial fibroblastsCancer-associated fibroblastsEmbryonicVitroExtracellular matrixCellsEpithelialSenescentInduceActivity in fibroblastsCardiac fibroblastLungHuman fibroblastDifferentiationMouse-Fibroblast GrowthProteinCAFsPathwayGene expressionTissueMacrophagesProteinsMethodsEndothelialCollagen productionRegulating fibroblast growthInducesAdult cardiac fibroblastsPlasma fibroblast treatmentCorrelated fibroblast densityApoptosisSenescenceSkinReceptorsTreatmentsRheumatoidRole of fibroblastsNumbers of fibroblastsConversion of fibroblastsCytokinesMetabolismInhibitionWrinklesProliferation of humanPhenotypes
Dermal fibroblasts18
- Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. (nih.gov)
- Methods RASF, rheumatoid arthritis dermal fibroblasts (RADF) and osteoarthritis synovial fibroblasts (OASF) were analysed for the expression of B cell survival/proliferating factors BAFF and APRIL in resting conditions and upon stimulation with TLR2/TLR3/TLR4 ligands. (bmj.com)
- Rat Dermal Fibroblasts (RDF) provided by Innoprot are isolated from Sprague Dawley rat skin by explants. (innoprot.com)
- Each vial of Rat Dermal Fibroblasts contains more than 500.000 viable cells. (innoprot.com)
- Innoprot also offers optimized medium and reagents for the growth of Rat Dermal Fibroblasts which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro RDF culture. (innoprot.com)
- Cultures of rat dermal fibroblasts are very useful for a wide range of cellular and molecular studies. (innoprot.com)
- Dermal fibroblasts also secrete large quantities of hyaluronan in response to inflammatory stimuli. (innoprot.com)
- During wound healing, dermal fibroblasts switch from a migratory, repopulating phenotype to a contractile, matrix-reassembling phenotype. (innoprot.com)
- There have been few studies to date of the phenotype of dermal fibroblasts in the presence of glyoxal, and our research shows that AGEs alter key properties of fibroblasts. (bmj.com)
- For the first time, we show that glycation can induce ectopic lipid accumulation through increased expression of PLIN2 in dermal fibroblasts, independently of the AMPK pathway. (bmj.com)
- In this study, we investigate the expression and the role of HDACs on the senescent phenotype of dermal fibroblasts. (aging-us.com)
- Moreover, treatment with SAHA, a histone deacetylase inhibitor (HDACi) also known as Vorinostat, or the specific downregulation of HDAC2 or HDAC7 by siRNA, induces the appearance of senescence biomarkers of dermal fibroblasts. (aging-us.com)
- Conversely, the ectopic re-expression of HDAC7 by lentiviral transduction in pre-senescent dermal fibroblasts extends their proliferative lifespan. (aging-us.com)
- SpinalCyte, a Texas-based tissue engineering technology company focused on regrowth of the spinal disc nucleus using human dermal fibroblasts, has been issued a new. (spinalnewsinternational.com)
- Background: There is little information on specific interactions between dermal fibroblasts and epidermal keratinocytes. (utwente.nl)
- Human Dermal Fibroblast-adult Cell Pellet (HDF-a Cell Pellet) is prepared from early passage Human Dermal Fibroblasts-adult. (sciencellonline.com)
- Transcriptomics evaluation of hexavalent chromium toxicity in human dermal fibroblasts. (cdc.gov)
- Therefore, maintaining the population of dermal fibroblasts is important for both preventing and treating age-related skin changes. (biomedcentral.com)
Synovial fibroblasts12
- Using next-generation sequencing to decipher the molecular mechanisms underlying aberrant rheumatoid arthritis synovial fibroblasts (RASF) activation, we performed transcriptome-wide RNA-seq and small RNA-seq on synovial fibroblasts from rheumatoid arthritis (RA) subject and normal donor. (mdpi.com)
- In the present study, we found that Kirenol inhibited the migration, invasion, and proinflammatory of IL-6 secretion of RA-associated synovial fibroblasts (FLS) at a concentration of 100-200 μg/ml in vitro . (frontiersin.org)
- Synovial fibroblasts (FLS) promote joint destruction via their attachment to the cartilage, and thus are key mediators of the pathogenesis of RA ( 2 ). (frontiersin.org)
- BCP crystals increase prostacyclin production and upregulate the prostacyclin receptor in OA synovial fibroblasts: potential effects on mPGES1 and MMP-13. (semanticscholar.org)
- Mechanism of basic calcium phosphate crystal-stimulated cyclo-oxygenase-1 up-regulation in osteoarthritic synovial fibroblasts. (semanticscholar.org)
- miR-146a overexpression decreases TRAF6 protein expression levels in primary human synovial fibroblasts. (figshare.com)
- B ) Western blot analysis showing significant decrease in the protein expression levels of TRAF6 in miR-146a overexpressed primary human synovial fibroblasts compared to controls and scrambled miR-146a. (figshare.com)
- Objectives To dissect the role of toll-like receptor (TLR) signalling and B cell survival/proliferating factors in the crosstalk between rheumatoid arthritis synovial fibroblasts (RASF) and B cells. (bmj.com)
- During osteoarthritis, synovial fibroblasts exposed to anomalous mechanical forces and an inflammatory microenvironment release factors such as a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) metalloproteinases that mediate tissue damage and perpetuate inflammation. (ucm.es)
- We therefore studied the production of ADAMTS by synovial fibroblasts and their contribution to cartilage degradation. (ucm.es)
- We reported that synovial fibroblasts constitutively express and release ADAMTS 4, 5, 7, and 12. (ucm.es)
- Therefore, synovial fibroblasts provide the biochemical tools to the chronicity and destruction of the osteoarthritic joints. (ucm.es)
Cancer-associated fibroblasts6
- In this study, we found that gene GGT5 was highly expressed in cancer-associated fibroblasts (CAFs) in lung adenocarcinoma, predicting the unfavorable survival of patients with lung adenocarcinoma. (aging-us.com)
- The PDAC tumor microenvironment (TME) is characterized by a dense desmoplastic stroma, dominated by cancer-associated fibroblasts (CAF), extracellular matrix (ECM) and immune cells displaying immunosuppressive phenotypes. (kcl.ac.uk)
- We postulated that cancer associated fibroblasts (CAFs) within the tumor stroma may exhibit subtype specific gene expression profiles and thus contribute to the biology of the disease in a subtype specific manner. (biomedcentral.com)
- However, breast cancer subtype-specific differences have not yet been reported for the tumor stromal cells even though multiple studies have shown that the gene expression profiles of breast cancer associated fibroblasts (CAFs) are distinctly different from their normal counterparts. (biomedcentral.com)
- Moreover, we discuss the impact of the cells responsible for this altered stromal collagen remodeling, the cancer associated fibroblasts (CAFs), and how these cells are key players in orchestrating the tumor microenvironment composition and tissue microarchitecture, hence also driving tumorigenesis and affecting response to treatment. (biomedcentral.com)
- The goal of this study is to investigate the role of cancer-associated fibroblasts and an extracellular matrix hyaluronic acids in the enhancement of metastatic liver tumor growth in ALD. (grantome.com)
Embryonic7
- Starting from a pool of nineteen candidate genes, we identified a combination of only three factors, Ascl1, Brn2 (also called Pou3f2) and Myt1l, that suffice to rapidly and efficiently convert mouse embryonic and postnatal fibroblasts into functional neurons in vitro. (nih.gov)
- Methods: Human keratinocytes (HEKa), and mouse embryonic fibroblasts (MEF) from the NC/Nga dermatitis prone mouse strain were used to investigate the induction of Th2-promoting cytokines (IL-33 and TSLP) and cell death by S. aureus. (bl.uk)
- Time lapse movie of mouse embryonic fibroblasts in culture imaged at 30 second intervals by phase contrast microscopy. (cellimagelibrary.org)
- By examining markers of different signaling pathways, and the differentiation behaviors of cells in the early embryonic brain, we infer that Gpc1(-/-) phenotypes most likely result from a transient reduction in fibroblast growth factor (FGF) signaling. (escholarship.org)
- Embryonic mouse fibroblasts (NIH‐3T3) spread on all arrays, and on contact with the substrate engulf nanopillars independently of the array pitch. (ntnu.no)
- Derived from day 12.5 post coitus fetuses of strain ICR mice and cultured as a monolayer in OriCell TM Mouse Embryonic Fibroblast Growth Medium (Cat. (cyagen.com)
- The ED50 as determined in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells is less than 3 ug/ml. (cusabio.com)
Vitro12
- Here we describe the reprogramming of fibroblasts into in vitro three-dimensional models of the human blastocyst, termed iBlastoids. (nature.com)
- The results of this in vitro study indicate that a very low BP dose (10 -9 M) can significantly affect the physiology of fibroblasts, increasing their proliferative capacity and modulating the expression of multiple genes involved in their growth and differentiation. (medsci.org)
- In this study, we investigated whether senescent human lung fibroblasts (LFs) and alveolar epithelial cells (AECs) could induce a senescent-like phenotype in "naïve" non-senescent LFs in vitro. (ubc.ca)
- As in vivo analysis of human pulmonary fibroblasts is not possible, the current authors designed an in vitro model of the local cytokine microenvironment of the diseased lung, using supernatants of stimulated epithelial and CD8+ T-cells. (ersjournals.com)
- The aim of the pilot study was the evaluation of the keratinised gingival fibroblast proliferation process after the use of Protefix® gel in vitro . (termedia.pl)
- An in vitro pilot study of human fibroblasts obtained from healthy donors and isolated from keratinised gingival tissues. (termedia.pl)
- In this in vitro pilot study, it was shown that Protefix® gel significantly stimulated the proliferation of human fibroblasts from primary culture. (termedia.pl)
- To evaluate the role of this transcription factor in cardiac fibroblasts (CFs) in diabetes mellitus, we performed a series of in vitro and in vivo experiments. (eurekamag.com)
- We determined the local composition and mechanical properties of the myocardium in established mouse models of genetic and acquired fibrosis and tested the effect of myocardial composition on cardiomyocyte contractility in vitro by systematically manipulating the number of fibroblasts and collagen concentration in a platform of engineered cardiac microtissues. (tue.nl)
- The in vitro results showed that while increasing collagen content had little effect on microtissue contraction, increasing fibroblast density caused a significant reduction in contraction force. (tue.nl)
- In vitro studies were then pursued with cultured isolated cardiac fibroblasts. (marshall.edu)
- In order to determine if mononuclear cells may be secreting factors capable of modulating fibroblast growth, the in vitro proliferative response of fibroblasts to cytokines known to be secreted by mononuclear cells was measured, using both growth arrested and proliferating cells. (edu.au)
Extracellular matrix16
- A fibroblast is a type of biological cell that synthesizes the extracellular matrix and collagen, produces the structural framework (stroma) for animal tissues, and plays a critical role in wound healing. (wikipedia.org)
- The main function of fibroblasts is to maintain the structural integrity of connective tissues by continuously secreting precursors of the extracellular matrix. (wikipedia.org)
- Fibroblasts secrete the precursors of all the components of the extracellular matrix, primarily the ground substance and a variety of fibers. (wikipedia.org)
- Fibroblasts, like the tumor-associated host fibroblasts (TAF), play a crucial role in immune regulation through TAF-derived extracellular matrix (ECM) components and modulators. (wikipedia.org)
- It occurs as a result of cardiac fibroblast (CFB) activation and differentiation into myofibroblasts, characterized by proliferation, extracellular matrix (ECM) production and stiffening, and contraction due to the expression of smooth muscle α-actin. (sigmaaldrich.com)
- Fibroblasts are responsible for forming and maintaining soft connective tissue and constitute the main source of collagen for the extracellular matrix (ECM). (medsci.org)
- Especially adhesion-related genes are upregulated in fibroblasts of chronic obstructive pulmonary disease patients, which can indicate a more pronounced role of fibroblasts in the inflammatory process in chronic obstructive pulmonary disease, possibly resulting in reduced function as effectors of extracellular matrix repair. (ersjournals.com)
- Fibroblasts are important in tissue repair and modulation by producing extracellular matrix (ECM) proteins, ECM-degrading enzymes and their inhibitors 9 . (ersjournals.com)
- Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. (innoprot.com)
- Fibrosis is mediated partly by extracellular matrix-depositing fibroblasts in the heart. (escholarship.org)
- We investigated the effect of its ethanol extract on hyaluronan (HA), an extracellular matrix compound with proinflammatory activity synthesis in human oral fibroblasts. (who.int)
- Fibroblasts are responsible for maintaining the extracellular matrix in healthy hearts, and differentiate into the wound-healing phenotype, myofibroblasts, following cardiac injury (3). (edu.au)
- Cancer-associated fibroblast (CAF) is a component of tumor microenvironment and can produce extracellular matrix (ECM), which could play an important role in cancer growth, invasion, and metastasis. (grantome.com)
- A cardiac fibroblast is a connective tissue cell in the heart which secretes an extracellular matrix rich in collagen and other macromolecules. (virtualflybrain.org)
- Fibroblasts play a key role in the production of these extracellular matrix components in the skin. (biomedcentral.com)
- Skin aging is the consequence of reduced numbers of fibroblasts, lower levels of extracellular matrix proteins, and decreased skin elasticity and tonus, thereby resulting in the formation of wrinkles[ 2 ]. (biomedcentral.com)
Cells61
- Fibroblasts are the most common cells of connective tissue in animals. (wikipedia.org)
- Fibroblasts can also migrate slowly over substratum as individual cells, again in contrast to epithelial cells. (wikipedia.org)
- While epithelial cells form the lining of body structures, it is fibroblasts and related connective tissues which sculpt the "bulk" of an organism. (wikipedia.org)
- Fibroblasts and fibrocytes are two states of the same cells, the former being the activated state, the latter the less active state, concerned with maintenance and tissue metabolism. (wikipedia.org)
- Like other cells of connective tissue, fibroblasts are derived from primitive mesenchyme. (wikipedia.org)
- In certain situations, epithelial cells can give rise to fibroblasts, a process called epithelial-mesenchymal transition (EMT). (wikipedia.org)
- Receptors on the surface of fibroblasts also allow regulation of hematopoietic cells and provide a pathway for immune cells to regulate fibroblasts. (wikipedia.org)
- Induction of pluripotent stem cells from adult human fibroblasts by defined factors. (nature.com)
- Cancer cells that were cultured with CAFs had down-regulated E-cadherin expression compared to cancer cells that were cultured with normal fibroblasts. (news-medical.net)
- Resident tissue fibroblasts, bone marrow-derived mesenchymal stem cells, and hematopoietic stem cells are examples of possible predecessors of CAFs. (news-medical.net)
- One method involves cancer cells reprogramming resident tissue fibroblasts to become CAFs, using small non-coding RNA molecules known as microRNAs or miRNAs (such as miR-31 and miR-214). (news-medical.net)
- Finally, bone marrow-derived mesenchymal stem cells (BM-MSCs) can alter secreted transforming growth factor-β1 (TGF-β1), which promotes the formation of CAFs from fibroblasts. (news-medical.net)
- Here we report that the administration of a set of five small molecules can chemically induce the transformation of fibroblasts into rod photoreceptor-like cells. (nature.com)
- These findings demonstrate that iPS cells can be generated from adult human fibroblasts. (nih.gov)
- Human CCD-1064Sk epithelial fibroblast cells were incubated in culture medium with 10 -5 , 10 -7 , or 10 -9 M zoledronate, alendronate, or ibandronate. (medsci.org)
- Fibroblasts proliferation was significantly increased at the lowest dose (10 -9 M) of each BP but was not affected at the higher doses (10 -5 and 10 -7 M). The proliferation increase may be related to the rise in TGF-β1 and TGFβR1 expression detected after the treatment of cells with 10 -9 M of zoledronate, alendronate, or ibandronate. (medsci.org)
- An association between IPF and cellular senescence is well established and several studies now describe a higher abundance of senescent fibroblasts and epithelial cells in the lungs of IPF patients compared with age-matched controls. (ubc.ca)
- We compare testosterone (T) metabolism in primary cultures of epithelial cells and fibroblasts separated from benign prostate hypertrophy (BPH) and prostate cancer tissues. (regrowth.com)
- The amounts of 5 alpha-dihydrotestosterone (DHT), formed by reduction of T by 5 alpha-reductase (5 alpha-R), were small: 5 and 2% (BPH) and 8 and 15% (adenocarcinoma) for epithelial cells and fibroblasts, respectively. (regrowth.com)
- The 5 alpha-reductase inhibitors, 4-MA (17 beta-(N,N-diethyl)carbamoyl-4-methyl-4-aza-5 alpha-androstan-3-one) and finasteride, inhibited DHT formation with a preferential action of 4-MA on epithelial cells (BPH or adenocarcinoma) and of finasteride on fibroblasts from adenocarcinoma. (regrowth.com)
- On the other hand, the lipido-sterol extract of Serenoa repens (LSESr, Permixon) inhibited the formation of all the T metabolites studied [IC50 S = 40 and 200 micrograms/ml (BPH) and 90 and 70 micrograms/ml (adenocarcinoma) in epithelial cells and fibroblasts, respectively]. (regrowth.com)
- In this study, we examined the sub-membrane mechanical structures of normal fibroblasts TIG-1 cells, and cervical cancer Hela cells using local elasticity mapping method of atomic force microscope. (scirp.org)
- In addition to ECM production, fibroblasts can be actively involved in the inflammatory processes via cytokine and chemokine production and by direct interaction with inflammatory cells 7 , 17 , 18 . (ersjournals.com)
- The data suggest that the malignant state of human fibroblasts is always associated with high levels of activity of receptor-bound u-PA, and in addition cells transformed to the malignant state are very likely to exhibit high levels of receptorbound t-PA and secreted forms of plasminogen activators. (aacrjournals.org)
- While untreated cells appeared fibroblast -like ( spindle or bundle shape), the alignment of exposed cells was altered into irregular polygonal configurations. (emf-portal.org)
- This is mainly because fibroblasts are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. (innoprot.com)
- Carcinoma-associated fibroblasts (CAFs) are abundant stromal cells in tumor microenvironment that are critically involved in cancer progression. (whiterose.ac.uk)
- Analysis of the cell states revealed different transcriptional programs in luminal epithelial cells (hormone receptor positive and secretory), basal epithelial cells (myoepithelial or basement-like), endothelial cells (lymphatic or vascular), macrophages (M1 or M2) and fibroblasts (three subgroups) and provided insight into progenitors of each cell types, she said. (medscape.com)
- For example, the proportion of fibroblasts was lower in 3 of the 11 patients, and even though the cells were pathologically normal, immune cell populations, including T-cells and macrophages, were also seen. (medscape.com)
- Results We found that the proliferative and migratory capacities of the cells were greatly reduced by glycation, which could be explained by an increase in fibroblast tensile strength. (bmj.com)
- Growth stimulatory factor originally isolated from bovine brain and pituitary and found to stimulate DNA synthesis in cultured fibroblast cells. (drugfuture.com)
- The increase of the inhibiting action of shaken serum on homologous fibroblasts is due possibly to the disappearance of a substance favoring the activity of homologous cells. (rupress.org)
- Exposure to hemin increased the gene and protein expression level of HO-1 in fibroblasts and diminished ROS levels, senescence, the inflammatory profile and simultaneously rescued mitochondria dysfunction by restoring mitophagy in COPD cells. (inserm.fr)
- We showed that Thy1(+)CD45(-)CD31(-)CD11b(-)Ter119(-) cells constitute the majority of cardiac fibroblasts. (escholarship.org)
- We used lineage tracing, transplantation studies, and parabiosis to show that most adult cardiac fibroblasts derive from the epicardium, a minority arises from endothelial cells, and a small fraction from Pax3-expressing cells. (escholarship.org)
- We did not detect generation of cardiac fibroblasts by bone marrow or circulating cells. (escholarship.org)
- In this study, it was investigated whether the expression of Thy-1 (CD90), a member of the immunoglobulin superfamily of adhesion molecules with constitutive expression on fibroblast cells, is modulated following infection with HCMV. (microbiologyresearch.org)
- Interestingly, after infection of fibroblasts with a recombinant GFP-expressing virus, infected as well as non-infected cells showed a reduced Thy-1 cell surface expression. (microbiologyresearch.org)
- Isolated from human kidney biopsies, these fibroblasts are adherent-dependent cells cultured on gelatin-precoated flasks and are transported in adherent form only. (kosheeka.com)
- Several years ago, a postdoctoral researcher working in Fu's lab was taking that approach, using a technique called siRNA to silence the PTB gene in connective tissue cells known as fibroblasts. (home.blog)
- We aimed to optimize a flow cytometric method described by other workers using endothelial cells to better differentiate between populations of fibroblasts in degrees of SA-β-gal activity. (vu.nl)
- Freshly dissociated fibroblasts were exposed to 30, 60 or 120 minutes of ischaemia by coating pelleted cells with mineral oil (modified from 5), followed by 60 minutes of reperfusion when mineral oil was replaced with culture media. (edu.au)
- IR of all durations evoked significant differentiation of fibroblasts into myofibroblasts, with 88±1% (mean±S.E.M., n=5) of cells differentiating following 30 minutes of ischaemia, 93±2% (n=4) following 60 minutes of ischaemia, and 92±1% (n=5) following 120 minutes of ischaemia. (edu.au)
- of cells differentiated from fibroblasts into myofibroblasts without IR. (edu.au)
- Fibroblasts are mesenchymal cells which perform many vital functions during development and in adulthood. (sciencellonline.com)
- Fibroblasts are one of the most accessible mammalian cell types and one of the easiest types of cells to grow in culture. (sciencellonline.com)
- By gently exfoliating the skin with electrostatic energy, the Dermavel Plasma Pen stimulates collagen and protein production in the fibroblast cells of the skin. (dermavel.com)
- The cytotoxicity effects for human fibroblasts were then compared with those reported for HCT 116 cells, and the findings point out that it is relevant to consider the cellular size. (edu.sa)
- Somatic mosaicism of an intragenic FANCB duplication in both fibroblast and peripheral blood cells observed in a Fanconi anemia patient leads to milder phenotype. (pacb.com)
- Fibroblasts are the most common biological cells that develop and maintain connective tissue in the body. (plasmapenuk.com)
- Gingival fibroblasts (GFs) present in the bone-lining mucosa have the capacity to activate the formation of osteoclasts, but little is known about which local immune cells (co-)mediate this process. (vumc.nl)
- After 21 days, comparable numbers of multinucleated cells (osteoclasts) were found in gingival fibroblast (GF)-PBMC and GF-monocyte cocultures. (vumc.nl)
- Fibroblasts constitute a dynamic and versatile population of cells of mesenchymal origin, implicated in both regenerative strategies and pathological conditions. (pedrogranja.net)
- This review intends to highlight the potential of fibroblasts in orchestrating tissue regeneration, as well as to contribute to uncover uncharted prospective applications of these cells. (pedrogranja.net)
- Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which sµggested a role in testicular embryogenesis Fibroblast Growth Factor 9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. (neobiolab.com)
- Furthermore, by developing a fibroblast stem cells (FSC)-enriched fibroblast focus model to mimic in vivo fibrogenic response, we demonstrated a dose-dependent increase in fibroblast focus formation and collagen production by primary lung fibroblasts treated with multi-walled carbon nanotubes (MWCNTs). (cdc.gov)
- In this study, we investigated the viability of human lung fibroblast-derived (HFL1) cells to different concentrations of cigarette smoke extract (CSE). (ladysilvia.it)
- GBA1 activities in both total lysate and PM of GD fibroblasts were low, and their relative percentages were similar to those of control cells. (unimi.it)
- The current study demonstrated that iPSC reprogramming in N2B27+2i/LIF culture converted chimpanzee fibroblasts into a multipotent cancerous state with unique gene expression, but not fully pluripotent stem cells. (elsevier.com)
- Plasma fibroblast therapy is a noninvasive way to stimulate the cells that produce collagen within your skin, called fibroblasts. (medphysique.com)
- The plasma fibroblast treatment uses naturally occurring plasma cells to break down the bonds between collagen and elastin in the skin. (aloterraenergy.com)
Epithelial1
- Conversely, fibroblasts in some situations may give rise to epithelia by undergoing a mesenchymal to epithelial transition (MET) and organizing into a condensed, polarized, laterally connected true epithelial sheet. (wikipedia.org)
Senescent7
- Lung fibroblasts from smokers and COPD patients displayed in long-term culture a senescent phenotype, characterized by a reduced replicative capacity, an increased senescence and inflammatory profile. (inserm.fr)
- These parameters were significantly higher in senescent COPD fibroblasts which also exhibited decreased mitochondrial activity (respiration, glycolysis, and ATP levels) which led to an increased production of ROS, and mitochondria biogenesis and impaired mitophagy process. (inserm.fr)
- The impact of senescent fibroblasts was further investigated in a pilot study using radiofrequency (RF) intervention for melasma. (ajou.ac.kr)
- This leads us to the speculation that senescent fibroblasts may contribute to drive melasma and might be considered as a potential therapeutic target. (ajou.ac.kr)
- Secretion of leukotrienes by senescent lung fibroblasts promotes pulmonary fibrosis. (instem.res.in)
- The LT-rich CM of senescent lung fibroblasts (IMR-90) induced profibrotic signaling in naive fibroblasts, which were abrogated by inhibitors of ALOX5, the principal enzyme in LT biosynthesis. (instem.res.in)
- Unlike human fibroblasts from unused donor lungs made senescent by irradiation, senescent IPF fibroblasts secreted LTs but failed to synthesize PGs. (instem.res.in)
Induce7
- Conclusions: S. aureus is unique amongst staphylococcal species in it's ability to induce an inflammatory response and cytotoxicity in human keratinocytes and mouse fibroblasts. (bl.uk)
- Research design and methods To investigate this, we treated cultured human fibroblasts with 0.6 mM glyoxal to induce acute glycation. (bmj.com)
- Background: To investigate whether monosodium urate (MSU) crystals induce interleukin (IL)-1β in human fibroblast-like synoviocytes (FLS), and whether the NLRP3 inflammasome is involved in the inflammatory mechanism. (harvard.edu)
- ROS production, known to induce senescence, is increased in COPD fibroblasts and mitochondria dysfunction participates in this process. (inserm.fr)
- We hypothesized that basic fibroblast growth factor might induce a more aggressive phenotype dependent on the amount of protein expressed in melanoma. (elsevier.com)
- To induce stress-induced senescence, fibroblasts were exposed to rotenone. (vu.nl)
- A synthetic elastase inhibitor failed to induce HGF, but α 1 -antitrypsin also stimulated HGF production in lung fibroblasts. (elsevier.com)
Activity in fibroblasts3
- G can have very low GAA activity in fibroblasts but express higher activity in muscle and store less glycogen in muscle than patients with classic-infantile Pompe disease. (eur.nl)
- Under stress-induced conditions the flow cytometric method but not the cytochemical method revealed significant higher SA-β-gal activity in fibroblasts from very old compared to young subjects (P = 0.004 and P = 0.635 respectively). (vu.nl)
- We studied the effects of two imino sugars, deoxynojirimycin (DNJ) and N-butyldeoxynojirimycin (NB-DNJ), on residual GAA activity in fibroblasts from eight patients with different forms of Pompe disease (two classic infantile, two non-classic infantile onset, four late-onset forms), and with different mutations of the GAA gene. (units.it)
Cardiac fibroblast4
- Mechanical regulation of cardiac fibroblast profibrotic phenotypes. (sigmaaldrich.com)
- Human Cardiac Fibroblast-adult ventricular total RNA (HCF-av tRNA) is prepared from early passage Human Cardiac Fibroblasts-adult ventricular using the Qiagen AllPrep DNA/RNA Mini kit. (3hbiomedical.com)
- We performed the following studies to examine whether MBG might directly stimulate cardiac fibroblast collagen production. (marshall.edu)
- The process in which a relatively unspecialized cell acquires the specialized structural and/or functional features of a cardiac fibroblast. (virtualflybrain.org)
Lung12
- A Senescence Bystander Effect in Human Lung Fibroblasts Waters, David W. (ubc.ca)
- The current authors explored the effects of the cytokine microenvironment on cell-cell interaction gene expression in pulmonary fibroblasts of controls (n = 6), and Global Initiative for Chronic Obstructive Lung Disease stage II (n = 7) and stage IV (n = 7) COPD patients. (ersjournals.com)
- The present data show that fibroblasts of chronic obstructive pulmonary disease patients display an altered response to the cytokine microenvironment, depending on both the disease stage and the central or peripheral location in the lung. (ersjournals.com)
- The current authors have previously observed that lung fibroblasts of patients with severe COPD exhibit an altered ECM production profile compared with fibroblasts of controls pointing to an important role of fibroblasts in the defective ECM modulation observed in COPD 8 , 10 - 12 . (ersjournals.com)
- The current study was conducted to further explore the role of fibroblasts in COPD and especially to investigate the influence of the complex cytokine microenvironment of the diseased lung on cell-to-cell interaction and matrix production. (ersjournals.com)
- Characterization of Distinct Populations of Carcinoma-Associated Fibroblasts from Non-Small Cell Lung Carcinoma Reveals a Role for ST8SIA2 in Cancer Cell Invasion. (whiterose.ac.uk)
- Chronic obstructive pulmonary disease (COPD) is associated with lung fibroblast senescence, a process characterized by an irreversible proliferation arrest associated with secretion of inflammatory mediators. (inserm.fr)
- Fibroblasts from smoker controls (S-C) and COPD patients were isolated from lung biopsies. (inserm.fr)
- Rat neonatal lung fibroblasts were isolated from the lung of adult SD rats. (ubpone.com)
- Here we report the positive regulation of hepatocyte growth factor (HGF) production in human lung fibroblasts exerted by SLPI or its C-terminal domain under physiologic concentrations (1 to 10 μM). (elsevier.com)
- In contrast, human skin fibroblasts exerted no SLPI-stimulated increase in HGF production, despite the fact that IL-1β increased HGF production with an intensity similar to that of human lung fibroblasts. (elsevier.com)
- Both the time-course and dose-response studies in human lung fibroblasts revealed that the induction of HGF messenger RNA (mRNA) and protein occurred in parallel, indicating that the mechanism existed at the steady-state mRNA level. (elsevier.com)
Human fibroblast4
- The induced proteins were identified as procollagenases by immunoprecipitation of induced medium with antibodies to purified human fibroblast collagenase. (elsevier.com)
- We analyzed the activities of CBE-sensitive β-glucosidase (GBA1) and AMP-DNM-sensitive β-glucosidase (GBA2) in total cell lysates and PM of human fibroblast cell lines from control (normal) subjects and from patients with GD clinical types 1, 2, and 3. (unimi.it)
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fibroblast Growth Factor 11 (FGF11) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (lipidx.org)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fibroblast Growth Factor 11 (FGF11) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (lipidx.org)
Differentiation4
- Continued accumulation and activity of fibroblasts along with differentiation into myofibroblasts, however, results in thickened, dense bands of highly aligned fibers. (aserf.org)
- IPC produced by 15 minutes ischaemia and 30 minutes reperfusion, prior to 60 minutes of ischaemia and 60 minutes of reperfusion, significantly reduced the differentiation of fibroblasts into myofibroblasts from 88±2% (n=7, no IPC) to 46±4% (n=7, IPC)(Fig. 1). (edu.au)
- These data indicate that IR injury strongly stimulates differentiation of cardiac fibroblasts into the wound-healing phenotype, the myofibroblast. (edu.au)
- IPC prevented IR-induced differentiation in a glibenclamide-sensitive manner, suggesting that activation of KATP channels is part of the mechanism by which IPC protects cardiac fibroblasts. (edu.au)
Mouse-Fibroblast Growth1
- Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fibroblast Growth Factor 11 (FGF11) in samples from serum, plasma or other biological fluids. (lipidx.org)
Protein11
- We tested 3 different targets, namely fibroblast activation protein (FAP), macrophages, and integrin α v β 3 . (snmjournals.org)
- Importantly, and in agreement with the recombinant protein work, an interaction between CaMKII and IKKβ was evident following Proximity Ligation Assays in adult cardiac fibroblasts. (strath.ac.uk)
- A protein called fibroblast growth factor 22 (or FGF22 for short) helps synapses to initially form within the hippocampus. (elifesciences.org)
- We have demonstrated the effects of retinoic acids of all-trans retinoic acid and 9-cis retinoic acid on the cell growth, alkaline phosphatase (ALPase) activity and protein synthesis of human deciduous and permanent periodontal ligments derived fibroblasts (HPLF-Y and HPFL,respectively). (nii.ac.jp)
- The data document that the killing of replicating mouse fibroblasts by etoposide represents an example of programmed cell death (apoptosis) that depends on protein synthesis. (elsevier.com)
- Fibroblast activity is enhanced by increases in store-operated Ca2+ entry (SOCE) and calcium release-activated calcium channel protein 1 (Orai1) levels. (elsevier.com)
- The main structural protein secreted by fibroblasts is collagen which helps to minimise fine lines, micro-furrows and wrinkles on the face and improves the elasticity, suppleness and hydration of our skin. (plasmapenuk.com)
- Heufelder, AE, Wenzel, BE & Bahn, RS 1992, ' Cell surface localization of a 72 kilodalton heat shock protein in retroocular fibroblasts from patients with Graves' ophthalmopathy ', Journal of Clinical Endocrinology and Metabolism , vol. 74, no. 4, pp. 732-736. (elsevier.com)
- Bahn, Rebecca S. / Cell surface localization of a 72 kilodalton heat shock protein in retroocular fibroblasts from patients with Graves' ophthalmopathy . (elsevier.com)
- Lyophilized Fibroblast Growth Factor-9 althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF9 Mouse Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. (neobiolab.com)
- Fibroblast activation protein-alpha and dipeptidyl peptidase IV (CD26): cell-surface proteases that activate cell signaling and are potential targets for cancer therapy. (uams.edu)
CAFs4
- fibroblasts (CAFs) are a major component of the cancer stroma. (news-medical.net)
- The conversion of CAFs from normal fibroblasts can occur in many ways. (news-medical.net)
- More recent research has discovered that the conversion of normal fibroblasts into CAFs involves the alteration of p53 from tumor-suppressive actions to tumor-supportive actions. (news-medical.net)
- Several studies have reported gene expression profile differences between CAFs and normal breast fibroblasts but in none of these studies were the results stratified based on tumor subtypes. (biomedcentral.com)
Pathway1
- The keratinocyte growth factor receptor or fibroblast growth factor receptor 2b (KGFR/FGFR2b) is activated by the specific interaction with the keratinocyte growth factor (KGF/FGF7), which targets the receptor to the degradative pathway, and the fibroblast growth factor 10 (FGF10/KGF2), which drives the receptor to the juxtanuclear recycling route. (uniroma1.it)
Gene expression3
- Manzano-Moreno FJ, Illescas-Montes R, Melguizo-Rodriguez L, Costela-Ruiz VJ, García-Martínez O, Ruiz C, Ramos-Torrecillas J. Impact of bisphosphonates on the proliferation and gene expression of human fibroblasts. (medsci.org)
- To determine differences in gene expression among CD26+ and CD26- fibroblasts from irradiated and non-irradiated breast capsules. (aserf.org)
- Furthermore, RNA-seq analysis demonstrated that Tie2-derived and Tbx18-derived fibroblasts within each operation group exhibit similar gene expression profiles. (escholarship.org)
Tissue11
- Though morphologically inconspicuous, ectopically transplanted fibroblasts can often retain positional memory of the location and tissue context where they had previously resided, at least over a few generations. (wikipedia.org)
- Tissue damage stimulates fibrocytes and induces the production of fibroblasts. (wikipedia.org)
- citation needed] Besides their commonly known role as structural components, fibroblasts play a critical role in an immune response to a tissue injury. (wikipedia.org)
- To determine whether a relationship exists among urokinase plasminogen activator (u-PA) activity, tissue plasminogen activator (t-PA) activity, and the malignant transformation of human fibroblasts, we measured receptor-bound and secreted u-PAs and t-PA activity in fibroblast cell strains of a unique cell lineage and compared the results with the values obtained in human fibrosarcoma-derived cell lines and control cell lines. (aacrjournals.org)
- GAA activity and glycogen content in fibroblasts and muscle tissue in healthy controls, adult patients with Pompe disease and classic-infantile patients were compared with those of the three index patients. (eur.nl)
- We found a fibroblast-like cell group composed of 6 clusters, 2 of which were validated for their enrichment in Aromhum LAM tissue. (northwestern.edu)
- Human Liver Fibroblasts are isolated from normal human liver tissue. (accegen.com)
- Human Liver Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Culture Complete Growth Medium generally for 3-7 days. (accegen.com)
- Gingival fibroblasts are the major constituents of gingival tissue and play a key role in their maintenance. (sciencellonline.com)
- When Plasma fibroblast is carried out by a trained professional in a clinical environment, it can provide skin tightening effects, improve skin texture, and encourage tissue regeneration. (abellasalon.net)
- Despite being frequently associated to disease development, particularly through the establishment of fibrotic tissue, fibroblasts hold great potential for tissue engineering and regenerative medicine applications. (pedrogranja.net)
Macrophages2
- Histologic studies have shown an accumulation of macrophages, lymphocytes, and fibroblasts, the latter of which lay down multi-directional collagen fibers during initial capsule formation. (aserf.org)
- IL-1β, TNFα produced by macrophages, IL-6 produced by fibroblasts). (biomedcentral.com)
Proteins6
- In human foreskin fibroblast cultures two proteins with M(r) 60,000 and 55,000 were found to be induced about 3.5-fold by epidermal growth factor (EGF), platelet-derived growth factor, and β-transforming growth factor. (elsevier.com)
- Two human melanoma cell lines, M14 and 1F6, known to have low endogenous basic fibroblast growth factor expression and slow growth as subcutaneous xenografts, were stably transfected with vectors encoding either the 18 kDa or all (ALL) isoform proteins of human basic fibroblast growth factor. (elsevier.com)
- Different clones overexpressing the 18 kDa or ALL basic fibroblast growth factor proteins were easily obtained. (elsevier.com)
- When the micro-damage occurs fibroblasts near the wound will multiply, travel to the site of damage and secrete collagen and other proteins to isolate the injury and facilitate wound repair. (plasmapenuk.com)
- Because several other parkinsonism-associated proteins have been connected to mitochondrial function and mitophagy, we studied the impact of endogenous mutations in ATPase type 13A2 (ATP13A2) on mitochondria in fibroblasts from KRS patients compared with controls. (uni.lu)
- Cell surface proteins of NIL1 hamster fibroblasts labeled by a galactose oxidase, tritiated borohydride method. (wikidata.org)
Methods5
- METHODS: Human cultured fibroblasts of three different donors and three different short-term human lymphocyte cultures were exposed to 1,950 MHz UMTS below the specific absorption rate (SAR) safety limit of 2 W/kg. (twoday.net)
- Methods: DNA was sequenced and GAA activity and glycogen content were measured in leukocytes, fibroblasts and muscle. (eur.nl)
- Methods: The effects of basic fibroblast growth factor on flexor tendon healing were evaluated with use of a canine model. (elsevier.com)
- Methods: Human keratinocytes were seeded on collagen matrices populated with increasing numbers of fibroblasts and cultured for 2 weeks at the air-liquid interface. (utwente.nl)
- The methods followed by SAGE données à l'appui des recommandations sont publiés en même and the processes for preparation of vaccine position temps que la note de synthèse. (who.int)
Endothelial2
- These include vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), stromal cell-derived factor 1 (SDF-1), fibroblast growth factor (FGF), and interleukin-6 (IL-6). (news-medical.net)
- Biological activity of the overexpressed basic fibroblast growth factor was confirmed in a human umbilical vein endothelial cell proliferation assay. (elsevier.com)
Collagen production2
- Marinobufagenin Stimulates Fibroblast Collagen Production and Causes F" by Jihad Elkareh, David J. Kennedy et al. (marshall.edu)
- This was associated with a significant attenuation of both basal and hypoxia/re-oxygenation-induced collagen production by the fibroblasts. (omeka.net)
Regulating fibroblast growth1
- Concentrated fish oil ameliorates non-alcoholic fatty liver disease by regulating fibroblast growth factor 21-adiponectin axis. (greenmedinfo.com)
Induces3
- The aim of this thesis was to elucidate the cellular and molecular mechanisms by which S. aureus induces it's pathogenic effects on keratinocyte and fibroblast cell lines. (bl.uk)
- Here we show that fibroblast growth factor 22 (FGF22), a target-derived presynaptic organizer in the mouse hippocampus, induces the expression of insulin-like growth factor 2 (IGF2) for the stabilization of presynaptic terminals. (elifesciences.org)
- Based on these findings, we propose that MBG directly induces increases in collagen expression by fibroblasts, and we suggest that this may be important in the cardiac fibrosis seen with experimental renal failure. (marshall.edu)
Adult cardiac fibroblasts1
- CaMKIIδ regulation of IκΒα degradation was explored in adult cardiac fibroblasts exposed to pharmacological stimulation. (strath.ac.uk)
Plasma fibroblast treatment3
- After a plasma fibroblast treatment, the targeted skin area will be red and swollen. (abellasalon.net)
- Is Plasma Fibroblast treatment safe and does it hurt? (empirebeauty.org)
- What can I expect after my Plasma Fibroblast treatment? (empirebeauty.org)
Correlated fibroblast density1
- Several studies have also correlated fibroblast density and cellular alignment with capsular contracture, and more recent reports have shown a functional difference in fibroblasts, with dysregulation of several inflammatory and fibrotic genes noted when comparing fibroblasts derived from non-contracted and contracted breast implant capsules (Kyle and Bayat 2015). (aserf.org)
Apoptosis11
- Hydrogen peroxide decreased human skin fibroblast viability with a concurrent increase in ROS generation and cell apoptosis. (spandidos-publications.com)
- Notably, TFPS pre‑treatment reduced oxidative stress and cell apoptosis in hydrogen peroxide‑treated skin fibroblasts. (spandidos-publications.com)
- These results indicated that TFPS alleviated hydrogen peroxide‑induced oxidative stress and apoptosis in skin fibroblasts via upregulation of SIRT1 expression, indicating that TFPS may act as a potential therapeutic agent for oxidative‑stress‑associated skin diseases and aging. (spandidos-publications.com)
- Farber, John L. / Programmed cell death (apoptosis) of mouse fibroblasts is induced by the topoisomerase II inhibitor etoposide . (elsevier.com)
- Oxidative stress has been suggested as a mechanism underlying skin aging, as it triggers apoptosis in various cell types, including fibroblasts, which play important roles in the preservation of healthy, youthful skin. (biomedcentral.com)
- In this study, we investigated the effect of (+)-catechin on apoptosis induced by oxidative stress in fibroblasts. (biomedcentral.com)
- The effect of (+)-catechin on cell viability, apoptosis, phosphorylation of c-Jun terminal kinases (JNK) and p38, and activation of caspase-3 in fibroblasts under oxidative stress were evaluated. (biomedcentral.com)
- Pretreatment of the fibroblasts with (+)-catechin inhibited hydrogen peroxide-induced apoptosis and reduced phosphorylation of JNK and p38 and activation of caspase-3. (biomedcentral.com)
- it reduces the number of skin fibroblasts by inducing apoptosis and decreasing their regenerative capacity, which in turn leads to increased skin sagging. (biomedcentral.com)
- Therefore, suppression of oxidative stress-induced apoptosis in skin fibroblasts is a potential treatment and prevention strategy for maintaining healthy youthful skin. (biomedcentral.com)
- In this study, we demonstrate that (+)-catechin has an inhibitory effect against oxidative stress-induced apoptosis in fibroblasts, accompanied by suppression of phosphorylation of p38 and c-Jun terminal kinases (JNK), both of which play an important role in intracellular apoptotic signaling induced by oxidative stress. (biomedcentral.com)
Senescence4
- The loss of p53 allows the fibroblasts to overcome senescence, enhances expression of CAF effectors, and promotes stromal and cancer cell expansion. (news-medical.net)
- Therefore, we investigated whether pharmacological induction of HO-1 by chronic hemin treatment attenuates senescence and improves dysfunctional mitochondria in COPD fibroblasts. (inserm.fr)
- We conclude that HO-1 attenuates senescence in COPD fibroblasts by protecting, at least in part, against mitochondria dysfunction and restoring mitophagy. (inserm.fr)
- We found the modified flow cytometric method measuring SA-β-gal activity superior in discriminating between degrees of senescence in different populations of fibroblasts. (vu.nl)
Skin42
- The aim of the present study was to investigate the protective effects of TFPS on hydrogen peroxide‑induced injury of human skin fibroblasts and to elucidate the aforementioned underlying mechanisms. (spandidos-publications.com)
- A hydrogen peroxide‑induced human skin fibroblast injury model was firstly established. (spandidos-publications.com)
- Here we describe an optimized protocol to generate iAstrocytes starting from skin fibroblasts and this approach can be adapted for a wide range of somatic cell types. (uu.nl)
- Treatment of fibroblasts with glyoxal reproduces some of the modifications found in the skin of diabetics. (bmj.com)
- A previous isolation of a human cytomegalovirus (CMV) from fibroblasts derived from intact skin of a Charcot-Marie-Tooth disease patient has prompted examination of six blind-coded cultured human skin lines by CMV DNA hybridization. (utmb.edu)
- 1 reported that primary cultures of human skin fibroblasts "possess muscarinic receptors with the properties of specific binding, saturability, pharmacologic specificity, inhibition of norepinephrine-stimulated adenylate cyclase, and increased binding after incubation with an antagonist. (elsevier.com)
- abstract = "To the Editor: In the July 26 issue, Nadi et al.1 reported that primary cultures of human skin fibroblasts "possess muscarinic receptors with the properties of specific binding, saturability, pharmacologic specificity, inhibition of norepinephrine-stimulated adenylate cyclase, and increased binding after incubation with an antagonist. (elsevier.com)
- Skin fibroblasts were isolated from young (mean age ± SD: 25.5 ± 1.8) and very old (age 90.2 ± 0.3) subjects. (vu.nl)
- Our regular price is 240.00 usd for … Fibroblast or plasma skin resurfacing is a collagen induction therapy. (web.app)
- Fibroblast is the world's most advanced, non-invasive, skin lifting, skin tightening and rejuvenation device used to treat wrinkles, and sagging, dull skin. (web.app)
- The Fibroblast Pen is an FDA approved, CE approved device used to perform fibroblast therapy, a technique used to stimulate production of collagen in the skin. (web.app)
- Ett alternativ till kirurgiskt ingrepp med effektivt Upper lower eye-lid lift before and after 10 days #Plasmapenresults by Dr. Mir Plasma Pen Fibroblast Technology is a great option for: Skin Tightening Med hjälp av plasmaljus stramas huden åt på ögonlocken. (web.app)
- Detta en helt ny behandling för uppstramning eller "Skin tightening" med plasmaimpulser. (web.app)
- The Fibroblast treatment uses a Plasma Pen to remove excess skin from the eyelid which causes hooding. (web.app)
- Fibroblast Skin Tightening. (web.app)
- These treatments are slightly technical, but the Milwaukee Specialists are … The Plasma Pen/Fibroblast Pen Skin Tightening treatment is an effective anti-aging solution for those who do not want to undergo invasive, surgical cosmetic procedures. (web.app)
- Treatments are Non Invasive Beauty treatments carried out by Certified Fibroblast Skin tightening Technicians and Certified Body Contouring Technicians. (premierfibroblast.com)
- PlasmaPen treats the fibroblasts in your skin more profoundly than a laser or a chemical peel can, leaving the top layer of your skin free of unsightly wounds. (mayallure.com)
- Plasma fibroblast is a non-surgical cosmetic procedure designed to improve the appearance of skin. (abellasalon.net)
- The skin rejuvenation results from plasma fibroblast rival those normally only achieved with invasive surgery. (abellasalon.net)
- While Plasma fibroblast Skin Tightening therapy is a relatively safe procedure, like with all cosmetic treatments, side effects are possible. (abellasalon.net)
- Those who are allergic to topical anesthetics, have a skin infection, or are using isotretinoin shouldn't get plasma fibroblast therapy. (abellasalon.net)
- Unfortunately, plasma fibroblast treatments are not as effective on darker skin tones. (abellasalon.net)
- Thee most important thing for those thinking about plasma fibroblast therapy is to find a skin clinic that is fully licensed and employs certified technicians who have experience with the technique. (abellasalon.net)
- Plasma skin tightening, Fibroblast Plasma, plasma pen, and Fibroblast are some of the names that this treatment goes by. (oldmillhigh.org)
- Plasma Fibroblast skin tightening is associated with success when it comes to tummy tightening, non-surgical eye lifts, and treatment for excess skin on different areas of the body. (oldmillhigh.org)
- What Areas Are Suitable For A Fibroblast Skin Tightening Treatment? (oldmillhigh.org)
- Fibroblast Skin Tightening is proven as a safe and non-invasive technique , provided it is done by a technician that is certified. (oldmillhigh.org)
- As we age, the dermis which is the middle layer of the skin where fibroblasts are found starts to thin which causes it to lose collagen and elastin. (plasmapenuk.com)
- Luckily, fibroblast skin tightening treatments are available to reduce the appearance of fine lines and wrinkles. (plasmapenuk.com)
- How do PlasmaPen fibroblast skin-tightening treatments work? (plasmapenuk.com)
- Fibroblast is the most Advanced Non Invasive Skin Tightening & Lifting for Skin Rejuvenation. (nkd-beauty.com)
- Fibroblast is a non-surgical treatment that uses a device to deliver the energy in the form of plasma to rejuvenate skin by improving facial lines & wrinkles, skin pigmentation associated with aging & other skin irregularities. (nkd-beauty.com)
- Plasma fibroblast is a non-surgical, skin-tightening procedure that rejuvenates the skin and has very little side effects or downtime. (smp-ink.com)
- The use of engineered skin equivalents consisting of organotypic cocultures of keratinocytes and fibroblasts offers an attractive approach for such studies. (utwente.nl)
- The expression of integrin α6β4 and of E-cadherin was comparable with that seen in native skin and was not significantly modulated by fibroblasts. (utwente.nl)
- Our Aurorae Pro is a high-quality beauty tool and is the only cosmetic non-laser device that reduces the volume of excess skin by harnessing the power of plasma fibroblast energy. (pnwmobilemassage.com)
- Keep your skin in tip top shape with the help of our premium plasma fibroblast skin tightening pen. (thegiftofsunshine.org)
- How will my skin look immediately after the Plasma Fibroblast skin Tightening treatment? (empirebeauty.org)
- Are there any potential side effects with the Plasma Fibroblast skin Tightening treatment? (empirebeauty.org)
- Learn about the fibroblast plasma treatment which uses microscopic plasma bubbles for targeted skin tightening. (aloterraenergy.com)
- The main benefit of the Fibroblast Plasma Treatment is that it is a very effective way to achieve skin tightening. (aloterraenergy.com)
Receptors3
- Fibroblast growth factor 21 (FGF21), an atypical member of the FGF family, functions as a hormone that coordinates lipid and glucose metabolism through binding of the FGF receptors in the presence of the coreceptor β -klotho [ 1 - 5 ]. (hindawi.com)
- It appears that the effects of melatonin on lipid metabolism in murine fibroblasts is mediated by melatonin membrane receptors. (nel.edu)
- Fibroblast growth factor receptors (FGFRs) 1-4 are involved in prostate cancer (PCa) regulation, but the role of FGFR-like 1 (FGFRL1) in PCa is unclear. (utu.fi)
Treatments3
- The discovery of these 2 potentially novel and unique hernia-associated fibroblasts may lead to the development of novel treatments that can nonsurgically prevent or reverse inguinal hernias. (northwestern.edu)
- Treatments provided by Premier Fibroblast Skintight are not medical treatments, nor use of medical grade equipment. (premierfibroblast.com)
- Are you wondering exactly how fibroblast treatments could help your clients? (plasmapenuk.com)
Rheumatoid2
- Enabled fibroblast-like synoviocytes (FLSs) plays a central role in synovial pannus formation and joint destruction in rheumatoid arthritis (RA). (synoviocytes.com)
- Fibroblast like synoviocytes (FLSs) exert important effects in the occurrence and progression of rheumatoid arthritis (RA). (synoviocytes.com)
Role of fibroblasts1
- The aim of this study was to elucidate the role of fibroblasts in bisphosphonate-related osteonecrosis of the jaw (BRONJ), evaluating the effect of zoledronate, alendronate, and ibandronate on the proliferation of fibroblasts and on their expression of genes essential for fibroblast physiology. (medsci.org)
Numbers of fibroblasts2
- With increasing numbers of fibroblasts incorporated into the collagen matrix, the expression of markers associated with keratinocyte activation, e.g. keratins 6, 16 and 17 and the cornified envelope precursor SKALP decreased, and involucrin localization shifted toward the granulosum layer. (utwente.nl)
- Conclusions: Our results illustrate that numbers of fibroblasts in the collagen matrix and their functional state is a critical factor for establishment of normal epidermal morphogenesis. (utwente.nl)
Conversion of fibroblasts2
- Reactive oxygen species (ROS) also promote the conversion of fibroblasts into highly migrating myofibroblasts via the accumulation of the hypoxia-inducible factor-1α (HIF-1α) transcription factor and the CXCL12 chemokine. (news-medical.net)
- Fig. 1: Conversion of fibroblasts and the molecular characterization of CiPCs. (nature.com)
Cytokines1
- These cytokines may influence the present fibroblasts, the key effectors in the defective ECM repair and maintenance in COPD. (ersjournals.com)
Metabolism6
- Previous studies have shown that fibroblast growth factor 23 (FGF23), which has an important role in phosphate metabolism, is elevated in HD patients. (scirp.org)
- The current study was undertaken to uncover the role of melatonin in lipid metabolism in the murine fibroblasts. (nel.edu)
- However, low levels of melatonin, with or without oleic acid, did not influence lipid metabolism in the cultured fibroblasts. (nel.edu)
- Fibroblast growth factor 23 (FGF-23) is a phosphate metabolism regulator in patients with chronic kidney disease (CKD). (indianjnephrol.org)
- Phosphoribosylpyrophosphate and purine metabolism in cultured fibroblasts. (jci.org)
- Studies on the metabolism and biological effects of nitropyrene and related nitro-polycyclic aromatic compounds in diploid human fibroblasts / Veronica M. Maher, Joe Dale Patton, and J. Justin McCormick. (who.int)
Inhibition1
- Primary adult rat cardiac fibroblasts were then used to study the effects of selective CaMKII inhibition on pharmacologically-induced NF-κB activation as well as interaction between CaMKII and specific IKK isoforms in a cardiac cellular setting. (strath.ac.uk)
Wrinkles4
- Eliminate fine lines, wrinkles, and more with Fibroblast. (scalpashop.com)
- Get trained with our intuitive online fibroblast training courses and learn how to instantly vanish the appearance of wrinkles. (scalpashop.com)
- Plasma fibroblast can also treat wrinkles on the face or neck, improve the appearance of scarring- including stretch marks, acne, and surgical scars. (smp-ink.com)
- Use the fibroblast plasma pen to remove wrinkles from your eyelids, eyebags, and even your forehead. (thegiftofsunshine.org)
Proliferation of human2
- The effects of different doses of beta radiation from a strontium-90 source on the proliferation of human Tenon's capsule fibroblasts were studied. (bmj.com)
- beta Radiation reduces the proliferation of human Tenon's capsule fibroblasts, and at higher doses this effect may be more pronounced one and two weeks after irradiation. (bmj.com)
Phenotypes1
- We elucidated the developmental origins of cardiac fibroblasts and characterized their corresponding phenotypes. (escholarship.org)