Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.
The collecting of fetal blood samples typically via ENDOSCOPIC ULTRASOUND GUIDED FINE NEEDLE ASPIRATION from the umbilical vein.
The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Exchange of substances between the maternal blood and the fetal blood at the PLACENTA via PLACENTAL CIRCULATION. The placental barrier excludes microbial or viral transmission.
In utero transfusion of BLOOD into the FETUS for the treatment of FETAL DISEASES, such as fetal erythroblastosis (ERYTHROBLASTOSIS, FETAL).
Transplacental passage of fetal blood into the circulation of the maternal organism. (Dorland, 27th ed)
Pathophysiological conditions of the FETUS in the UTERUS. Some fetal diseases may be treated with FETAL THERAPIES.
The process by which fetal Rh+ erythrocytes enter the circulation of an Rh- mother, causing her to produce IMMUNOGLOBULIN G antibodies, which can cross the placenta and destroy the erythrocytes of Rh+ fetuses. Rh isoimmunization can also be caused by BLOOD TRANSFUSION with mismatched blood.
A condition characterized by the abnormal presence of ERYTHROBLASTS in the circulation of the FETUS or NEWBORNS. It is a disorder due to BLOOD GROUP INCOMPATIBILITY, such as the maternal alloimmunization by fetal antigen RH FACTORS leading to HEMOLYSIS of ERYTHROCYTES, hemolytic anemia (ANEMIA, HEMOLYTIC), general edema (HYDROPS FETALIS), and SEVERE JAUNDICE IN NEWBORN.
A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).
Endoscopic examination, therapy or surgery of the fetus and amniotic cavity through abdominal or uterine entry.
A clear, yellowish liquid that envelopes the FETUS inside the sac of AMNION. In the first trimester, it is likely a transudate of maternal or fetal plasma. In the second trimester, amniotic fluid derives primarily from fetal lung and kidney. Cells or substances in this fluid can be removed for prenatal diagnostic tests (AMNIOCENTESIS).
The heart rate of the FETUS. The normal range at term is between 120 and 160 beats per minute.
Determination of the nature of a pathological condition or disease in the postimplantation EMBRYO; FETUS; or pregnant female before birth.
Deficient oxygenation of FETAL BLOOD.
The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated as the time from the last day of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization.
Abnormal accumulation of serous fluid in two or more fetal compartments, such as SKIN; PLEURA; PERICARDIUM; PLACENTA; PERITONEUM; AMNIOTIC FLUID. General fetal EDEMA may be of non-immunologic origin, or of immunologic origin as in the case of ERYTHROBLASTOSIS FETALIS.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
Specialized arterial vessels in the umbilical cord. They carry waste and deoxygenated blood from the FETUS to the mother via the PLACENTA. In humans, there are usually two umbilical arteries but sometimes one.
The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc.
The circulation of BLOOD, of both the mother and the FETUS, through the PLACENTA.
The failure of a FETUS to attain its expected FETAL GROWTH at any GESTATIONAL AGE.
The last third of a human PREGNANCY, from the beginning of the 29th through the 42nd completed week (197 to 294 days) of gestation.
The weight of the FETUS in utero. It is usually estimated by various formulas based on measurements made during PRENATAL ULTRASONOGRAPHY.
The process of bearing developing young (EMBRYOS or FETUSES) in utero in non-human mammals, beginning from FERTILIZATION to BIRTH.
The flexible rope-like structure that connects a developing FETUS to the PLACENTA in mammals. The cord contains blood vessels which carry oxygen and nutrients from the mother to the fetus and waste products away from the fetus.
Monitoring of FETAL HEART frequency before birth in order to assess impending prematurity in relation to the pattern or intensity of antepartum UTERINE CONTRACTION.
The visualization of tissues during pregnancy through recording of the echoes of ultrasonic waves directed into the body. The procedure may be applied with reference to the mother or the fetus and with reference to organs or the detection of maternal or fetal disease.
Death of the developing young in utero. BIRTH of a dead FETUS is STILLBIRTH.
Cells derived from a FETUS that retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.
Hemorrhage caused by vitamin K deficiency.
The middle third of a human PREGNANCY, from the beginning of the 15th through the 28th completed week (99 to 196 days) of gestation.
Measurement of oxygen and carbon dioxide in the blood.
The repetitive uterine contraction during childbirth which is associated with the progressive dilation of the uterine cervix (CERVIX UTERI). Successful labor results in the expulsion of the FETUS and PLACENTA. Obstetric labor can be spontaneous or induced (LABOR, INDUCED).
The major component of hemoglobin in the fetus. This HEMOGLOBIN has two alpha and two gamma polypeptide subunits in comparison to normal adult hemoglobin, which has two alpha and two beta polypeptide subunits. Fetal hemoglobin concentrations can be elevated (usually above 0.5%) in children and adults affected by LEUKEMIA and several types of ANEMIA.
A group of inherited disorders characterized by structural alterations within the hemoglobin molecule.
Contagious infection with human B19 Parvovirus most commonly seen in school age children and characterized by fever, headache, and rashes of the face, trunk, and extremities. It is often confused with rubella.
Ultrasonography applying the Doppler effect, with frequency-shifted ultrasound reflections produced by moving targets (usually red blood cells) in the bloodstream along the ultrasound axis in direct proportion to the velocity of movement of the targets, to determine both direction and velocity of blood flow. (Stedman, 25th ed)
An infant during the first month after birth.
The heart of the fetus of any viviparous animal. It refers to the heart in the postembryonic period and is differentiated from the embryonic heart (HEART/embryology) only on the basis of time.
Percutaneous transabdominal puncture of the uterus during pregnancy to obtain amniotic fluid. It is commonly used for fetal karyotype determination in order to diagnose abnormal fetal conditions.
A pathologic condition of acid accumulation or depletion of base in the body. The two main types are RESPIRATORY ACIDOSIS and metabolic acidosis, due to metabolic acid build up.
A variety of anesthetic methods such as EPIDURAL ANESTHESIA used to control the pain of childbirth.
Passage of blood from one fetus to another via an arteriovenous communication or other shunt, in a monozygotic twin pregnancy. It results in anemia in one twin and polycythemia in the other. (Lee et al., Wintrobe's Clinical Hematology, 9th ed, p737-8)
Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.
Morphological and physiological development of FETUSES.
The number of RED BLOOD CELLS per unit volume in a sample of venous BLOOD.
A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.
The threadlike, vascular projections of the chorion. Chorionic villi may be free or embedded within the DECIDUA forming the site for exchange of substances between fetal and maternal blood (PLACENTA).
Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.
The beginning third of a human PREGNANCY, from the first day of the last normal menstrual period (MENSTRUATION) through the completion of 14 weeks (98 days) of gestation.
Morphological and physiological development of EMBRYOS or FETUSES.
The hollow thick-walled muscular organ in the female PELVIS. It consists of the fundus (the body) which is the site of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus, are the FALLOPIAN TUBES.
Rhythmic, intermittent propagation of a fluid through a BLOOD VESSEL or piping system, in contrast to constant, smooth propagation, which produces laminar flow.
The volume of packed RED BLOOD CELLS in a blood specimen. The volume is measured by centrifugation in a tube with graduated markings, or with automated blood cell counters. It is an indicator of erythrocyte status in disease. For example, ANEMIA shows a low value; POLYCYTHEMIA, a high value.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
The visualization of deep structures of the body by recording the reflections or echoes of ultrasonic pulses directed into the tissues. Use of ultrasound for imaging or diagnostic purposes employs frequencies ranging from 1.6 to 10 megahertz.
Extraction of the FETUS by means of abdominal HYSTEROTOMY.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
The oxygen-carrying proteins of ERYTHROCYTES. They are found in all vertebrates and some invertebrates. The number of globin subunits in the hemoglobin quaternary structure differs between species. Structures range from monomeric to a variety of multimeric arrangements.
Volume of circulating BLOOD. It is the sum of the PLASMA VOLUME and ERYTHROCYTE VOLUME.
Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA).
An anterior pituitary hormone that stimulates the ADRENAL CORTEX and its production of CORTICOSTEROIDS. ACTH is a 39-amino acid polypeptide of which the N-terminal 24-amino acid segment is identical in all species and contains the adrenocorticotrophic activity. Upon further tissue-specific processing, ACTH can yield ALPHA-MSH and corticotrophin-like intermediate lobe peptide (CLIP).
Progenitor cells from which all blood cells derive.
Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.
The flow of BLOOD through or around an organ or region of the body.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
Glucose in blood.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.

Cocaine metabolite kinetics in the newborn. (1/4377)

The study goal was to determine the half-life elimination of cocaine and benzoylecgonine (BZE) in the newborn. Three 0.3-mL blood samples were collected during the first day of life. Urine was collected once daily. Cocaine and BZE concentrations were determined by gas chromatography-mass spectrometry. An extraction method was developed for measuring low concentrations of cocaine and BZE in small (0.1 mL) blood samples. Cocaine had a half-life of 11.6 h in one subject. The half-life of BZE during the first day of life, based on blood data in 13 subjects, was 16 h (95% confidence interval [CI], 12.8 to 21.4 h). The half-life of BZE during the first week of life, based on urine data in 16 subjects, was 11.2 h (95% CI, 10.1 to 11.8 h). The novel extraction method for small blood sample volumes should be applicable to other basic drugs.  (+info)

Cortisol in fetal fluids and the fetal adrenal at parturition in the tammar wallaby (Macropus eugenii). (2/4377)

Glucocorticoid hormones may play a critical role in initiating parturition in tammar wallabies. In this study, we investigated the concentration of cortisol in fetal fluids and cortisol production by fetal adrenals over the last 3 days of the 26-day pregnancy and within 24 h postpartum. The fetal adrenals almost doubled in size between Days 24 and 26 of pregnancy, and their cortisol content increased over 10-fold during this period, from 10 pg to over 100 pg per adrenal pair. After birth, neonatal adrenals continued to grow, but cortisol content fell dramatically to 20 pg. The prepartum increase in adrenal cortisol was reflected by a substantial rise in cortisol concentrations in yolk sac fluid, allantoic fluid, and fetal blood, which were below 10 ng/ml on Day 24 and rose to over 40 ng/ml by Day 26. Cortisol concentrations in neonatal blood decreased postpartum, mirroring decreased cortisol content in neonatal adrenals. Cortisol production by the fetal adrenal was stimulated in vitro by ACTH and prostaglandin E2, suggesting that the in vivo increase may be stimulated by release of ACTH from the fetal hypothalamic-pituitary axis and prostaglandin E2 from the placenta. These results indicate that increasing cortisol production by the fetal adrenal is a characteristic of late pregnancy in the tammar wallaby and support the suggestion that fetal cortisol may trigger the initiation of parturition in this marsupial species.  (+info)

Delay of preterm delivery in sheep by omega-3 long-chain polyunsaturates. (3/4377)

A positive correlation has been shown between dietary intake of long-chain omega-3 fatty acids in late pregnancy and gestation length in pregnant women and experimental animals. To determine whether omega-3 fatty acids have an effect on preterm labor in sheep, a fish oil concentrate emulsion was continuously infused to six pregnant ewes from 124 days gestational age. At 125 days, betamethasone was administered to the fetus to produce preterm labor. Both the onset of labor and the time of delivery were delayed by the fish oil emulsion. Two of the omega-3-infused ewes reverted from contractions to nonlabor, an effect never previously observed for experimental glucocorticoid-induced preterm labor in sheep. Maternal plasma estradiol and maternal and fetal prostaglandin E2 rose in control ewes but not in those infused with omega-3 fatty acid. The ability of omega-3 fatty acids to delay premature delivery in sheep indicates their possible use as tocolytics in humans. Premature labor is the major cause of neonatal death and long-term disability, and these studies present information that may lead to a novel therapeutic regimen for the prevention of preterm delivery in human pregnancy.  (+info)

Phenotypic and functional evidence for the expression of CXCR4 receptor during megakaryocytopoiesis. (4/4377)

The identification of stromal cell-derived factor (SDF)-1alpha as a chemoattractant for human progenitor cells suggests that this chemokine and its receptor might represent critical determinants for the homing, retention, and exit of precursor cells from hematopoietic organs. In this study, we investigated the expression profile of CXCR4 receptor and the biological activity of SDF-1alpha during megakaryocytopoiesis. CD34(+) cells from bone marrow and cord blood were purified and induced to differentiate toward the megakaryocyte lineage by a combination of stem-cell factor (SCF) and recombinant human pegylated megakaryocyte growth and development factor (PEG-rhuMGDF). After 6 days of culture, a time where mature and immature megakaryocytes were present, CD41(+) cells were immunopurified and CXCR4mRNA expression was studied. High transcript levels were detected by a RNase protection assay in cultured megakaryocytes derived from cord blood CD34(+) cells as well as in peripheral blood platelets. The transcript levels were about equivalent to that found in activated T cells. By flow cytometry, a large fraction (ranging from 30% to 100%) of CD41(+) cells showed high levels of CXCR4 antigen on their surface, its expression increasing in parallel with the CD41 antigen during megakaryocytic differentiation. CXCR4 protein was also detected on peripheral blood platelets. SDF-1alpha acts on megakaryocytes by inducing intracellular calcium mobilization and actin polymerization. In addition, in in vitro transmigration experiments, a significant proportion of megakaryocytes was observed to respond to this chemokine. This cell migration was inhibited by pertussis toxin, indicating coupling of this signal to heterotrimeric guanine nucleotide binding proteins. Although a close correlation between CD41a and CXCR4 expession was observed, cell surface markers as well as morphological criteria indicate a preferential attraction of immature megakaryocytes (low level of CD41a and CD42a), suggesting that SDF-1alpha is a potent attractant for immature megakaryocytic cells but is less active on fully mature megakaryocytes. This hypothesis was further supported by the observation that SDF-1alpha induced the migration of colony forming unit-megakaryocyte progenitors (CFU-MK) and the expression of activation-dependent P-selectin (CD62P) surface antigen on early megakaryocytes, although no effect was observed on mature megakaryocytes and platelets. These results indicate that CXCR4 is expressed by human megakaryocytes and platelets. Furthermore, based on the lower responses of mature megakaryocytes and platelets to SDF-1alpha as compared with early precursors, these data suggest a role for this chemokine in the maintenance and homing during early stages of megakaryocyte development. Moreover, because megakaryocytes are also reported to express CD4, it becomes important to reevaluate the role of direct infection of these cells by the human immunodeficiency virus (HIV)-1 in HIV-1-related thrombocytopenia.  (+info)

Detection of antibody to bovine syncytial virus and respiratory syncytial virus in bovine fetal serum. (5/4377)

Batches of commercial fetal bovine serum, described by the suppliers as antibody-free, all contained antibody to bovine syncytial virus (BSV) when tested by indirect immunofluorescence. Antibody to bovine respiratory syncytial virus (RSV) was not detected in these sera. Twenty-four percent of individual fetal bovine sera contained antibody to BSV, and 14% contained antibody to RSV when tested by indirect immunofluorescence. BSV antibody titers in fetal sera from dams with high BSV antibody levels were variable but always higher than RSV antibody titers. Radial immunodiffusion studies with BSV-positive sera revealed the presence of immunoglobulin M (IgM), IgG, and IgA, but the quantity of these immunoglobulins was not directly related to the BSV antibody titers. The evidence suggests that the antibody present in fetal sera arose as the result of infection rather than from maternal transfer across the placenta.  (+info)

Early ontogeny of monocytes and macrophages in the pig. (6/4377)

Prenatal development of cord blood monocytes and tissue macrophages was studied in pig foetuses by immunophenotyping and functional assays. The function of peripheral blood monocytes was compared in germ-free and conventional piglets. First macrophages were identified by electron microscopy in foetal liver on the 25th day of gestation. Monoclonal antibodies against porcine CD45 and SWC3 antigens were used for flow cytometric identification of myelomonocytic cells in cell suspensions prepared from the yolk sac, foetal liver, spleen and cord blood. Leukocytes expressing the common myelomonocytic antigen SWC3 were found in all organs studied since the earliest stages of development. Opsonized zymosan ingestion assay was used to determine the phagocytic capacity of foetal mononuclear phagocytes isolated from cord blood, liver and spleen. In the foetal liver, avid phagocytosis of apoptic cells had been found to occur before cells were able to ingest zymosan in vitro. The first cells capable of ingesting zymosan particles were found on the 40th day of gestation in umbilical blood and 17 days later in foetal spleen and liver. Their relative proportion increased with age. Cord blood monocytes and peripheral blood monocytes in germ-free piglets had low oxidatory burst activity as shown by iodonitrophenyl tetrazolium reduction assay. A remarkable increase of oxidatory burst activity was observed in conventional piglets, probably due to activation of immune mechanisms by the microflora colonizing gastrointestinal tract.  (+info)

Laboratory assay reproducibility of serum estrogens in umbilical cord blood samples. (7/4377)

We evaluated the reproducibility of laboratory assays for umbilical cord blood estrogen levels and its implications on sample size estimation. Specifically, we examined correlation between duplicate measurements of the same blood samples and estimated the relative contribution of variability due to study subject and assay batch to the overall variation in measured hormone levels. Cord blood was collected from a total of 25 female babies (15 Caucasian and 10 Chinese-American) from full-term deliveries at two study sites between March and December 1997. Two serum aliquots per blood sample were assayed, either at the same time or 4 months apart, for estrone, total estradiol, weakly bound estradiol, and sex hormone-binding globulin (SHBG). Correlation coefficients (Pearson's r) between duplicate measurements were calculated. We also estimated the components of variance for each hormone or protein associated with variation among subjects and variation between assay batches. Pearson's correlation coefficients were >0.90 for all of the compounds except for total estradiol when all of the subjects were included. The intraclass correlation coefficient, defined as a proportion of the total variance due to between-subject variation, for estrone, total estradiol, weakly bound estradiol, and SHBG were 92, 80, 85, and 97%, respectively. The magnitude of measurement error found in this study would increase the sample size required for detecting a difference between two populations for total estradiol and SHBG by 25 and 3%, respectively.  (+info)

Long-term fetal microchimerism in peripheral blood mononuclear cell subsets in healthy women and women with scleroderma. (8/4377)

Fetal CD34(+) CD38(+) cells have recently been found to persist in maternal peripheral blood for many years after pregnancy. CD34(+) CD38(+) cells are progenitor cells that can differentiate into mature immune-competent cells. We asked whether long-term fetal microchimerism occurs in T lymphocyte, B lymphocyte, monocyte, and natural-killer cell populations of previously pregnant women. We targeted women with sons and used polymerase chain reaction for a Y-chromosome-specific sequence to test DNA extracted from peripheral blood mononuclear cells (PBMC) and from CD3, CD19, CD14, and CD56/16 sorted subsets. We also asked whether persistent microchimerism might contribute to subsequent autoimmune disease in the mother and included women with the autoimmune disease scleroderma. Scleroderma has a peak incidence in women after childbearing years and has clinical similarities to chronic graft-versus-host disease that occurs after allogeneic hematopoietic stem-cell transplantation, known to involve chimerism. Sixty-eight parous women were studied for male DNA in PBMC and 20 for PBMC subsets. Microchimerism was found in PBMC from 33% (16 of 48) of healthy women and 60% (12 of 20) women with scleroderma, P =.046. Microchimerism was found in some women in CD3, CD19, CD14, and CD56/16 subsets including up to 38 years after pregnancy. Microchimerism in PBMC subsets was not appreciably more frequent in scleroderma patients than in healthy controls. Overall, microchimerism was found in CD3, CD19, and CD14 subsets in approximately one third of women and in CD56/16 in one half of women. HLA typing of mothers and sons indicated that HLA compatibility was not a requirement for persistent microchimerism in PBMC subsets. Fetal microchimerism in the face of HLA disparity implies that specific maternal immunoregulatory pathways exist that permit persistence but prevent effector function of these cells in normal women. Although microchimerism in PBMC was more frequent in women with scleroderma than healthy controls additional studies will be necessary to determine whether microchimerism plays a role in the pathogenesis of this or other autoimmune diseases.  (+info)

IND Applications for Minimally Manipulated, Unrelated Allogeneic Placental/Umbilical Cord Blood Intended for Hematopoietic and Immunologic Reconstitution in Patients with Disorders Affecting the Hematopoietic System, CBER, Biologics
Background: Cell therapy is a potential therapeutic approach for neurodegenerative disorders. Human umbilical cord bloodderived mesenchymal stem cells (hUCB-MSCs) are an appropriate source of stem cells for use in various cell-based therapies. Objectives: In this study, we examined a real-time PCR approach for neural differentiation of hUCB-MSCs in vitro. Materials and Methods: MSCs were cultured in DMEM medium supplemented with 10% FBS in a humidified incubator equilibration at 5% CO2 and 37°C. For the neural differentiation of MSCs, the DMEM was removed and replaced with pre-induction media (retinoic acid [RA], basic fibroblast growth factor [bFGF], and epidermal growth factor [EGF]) and basal medium for two days. They were then cultured in nerve growth factor (NGF), 3-isobutyl-1-methylxanthine (IBMX), ascorbic acid (AA), and basal medium for six days. We also monitored the expression of markers for neural differentiation with real-time PCR. Results: The results of real-time PCR showed that the
Although in several studies IL15 was an effective cytokine that could induce eradication of experimental tumors (8-11), application of MSCs to serve as a vehicle for delivering IL15 to cancer has not been reported so far. In this study, we investigated the development of IL15 gene modified MSCs for tumor therapy strategy. The hypothesis is that MSCs secreting IL15, when injected into tumor-bearing mice, would specifically migrate to and enrich in the tumor site, and exert a potent antitumor immunity. The results presented here support feasibility of this strategy.. Accumulative evidences demonstrate that MSCs are able to migrate to tumor site or inflammatory region (31, 32). In this study, histology analysis showed accumulation of MSCs in tumor site, but not in other organ sites. Higher level of IL15 was observed in tumor site, and systemic level of IL15 was significantly lower than tumor local level of IL15. These findings indicated that MSC-IL15 was able to specific home to tumor tissue, and a ...
Front Pharmacol. 2017 Sep 8 Human Umbilical Cord Blood Cell Transplantation in Neuroregenerative Strategies. Galieva LR1, Mukhamedshina YO1,2, Arkhipova SS1, Rizvanov AA1. Author information Abstract At present there is no effective treatment of pathologies associated with the death of neurons and glial cells which take place as a result of physical trauma or ischemic lesions of the nervous system. Thus, researchers have high hopes for a treatment based on the use of stem cells (SC),
Diabetes mellitus (DM) instigates a cascade of events leading to vascular damage and poor recovery after ischemic stroke. Our previous studies have found that treatment of stroke with bone-marrow-stromal cells initiated at 24h after stroke improves functional recovery in non-DM rats, but not in DM rats. Effective therapy for stroke in the non-DM population may not necessarily transfer to the DM population, prompting the need to develop therapeutic approaches specifically designed to reduce neurological deficits after stroke in the DM population. In this study, we hypothesize that treatment of stroke with human umbilical cord blood cells (HUCBCs) promotes neurorestorative effect in DM rats.. Methods: Type one DM (T1DM) was induced with a single injection of streptozotocin (60 mg/kg, ip) to adult male Wistar rats. These rats were subjected to 2h MCAo and were randomized to intravenous injection via tail-vein with: 1) phosphate-buffered saline control; 2) HUCBCs (5x106) at 24 hours after MCAo. A ...
In this study, after 21 days of ceasing CCl 4 , both placebo-treated and untreated cirrhotic mice showed reduced liver damage as indicated by a decrease of AST/ALT index, recovery of histology, and reduction of fibrosis-related gene expression. Presumably, the liver of these mice were capable of self-regeneration. The cause of the self-regeneration has been explained by the ceasing of CCl 4 administration during the 21 days of treatment. Other studies have shown similar results to our study Carvalho et al., 2008 Kuo et al., 2008 .. However, our study uniquely demonstrates that stem cell treatment in cirrhotic mice was better for restoring liver function and histology (significantly better than placebo treatment). The effects of stem cell treatment in our mouse model of liver cirrhosis may be attributed to stem cell immunomodulation, stem cell homing and differentiation, and secreted cytokines Berardis et al., 2015 .. In terms of stem cell therapy for liver cirrhosis, there are several routes for ...
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
Robust and large-scale expansion of umbilical cord blood stem cells in vitro is necessary for widening the usage of transplantation therapies for the treatment of hematological and immune diseases. The lack of understanding of the complex inter-cellular networks regulating stem cell fate in culture explains the low success met so far for the ex vivo expansion of hematopoietic stem cells. The development of a mathematical model of in vitro hematopoiesis coupled with gene expression profiling led to predictions about the secreted factors that play a crucial role in regulating hematopoietic stem cell self-renewal in culture. We tested 18 putative molecules predicted to display effects on primitive progenitor (Long-Term Culture-Initiating Cell; LTC-IC) output, functionally validating three stimulators (VEGF, PDGF, EGF) and three inhibitors (TGFβ, CCL4, CXCL10). Combinatorial studies with the stimulatory molecules showed less-than additive effects, perhaps related to redundant signaling mechanisms. Small
Umbilical cord blood (UCB) is an alternative hematopoietic stem cell (HSC) source that can ameliorate several diseases through transplantation. The purpose of this project is to analyze clinical studies comparing HSCs from a single cord blood unit (CBU) to HSCs from bone marrow, and to explore methods of increasing limited amounts of HSCs. It was found that UCB transplantation in adults is a viable method when a matched bone marrow transplant cannot be identified. Further clinical studies using two CBUs suggest better engraftment and lower risk of relapse. However, double cord blood transplantation has been faced with the challenge of single unit dominance in most studies. Ex vivo expansion of UCB HSCs is another promising method to overcome limited HSC counts.
CD34+ cells were isolated from a total of 27 cord blood samples, and the number of MNCs as well as CD34+ cells was analyzed in a Neubauer chamber. As shown in Table 1, the concentration of CD34+ cells was 28 cells/mm3 of cord blood (yield after Ficoll and MACS procedure). The purity of this cell fraction was 93.2 ± 3.6% (87-99%; N = 10). Since 22.2 ± 11.3% (12.5-50.6%) of the CD34+ cells were dead, the average frequency of viable CD34+ among total cells was 71.0 ± 13.1% (39.4-86.5%).. CD34+ cells were cultured with three combinations of growth factors (Figure 1). With TPO + FL + KL, the average increase in cell number in 9 samples studied was 3.55 ± 1.6-fold after 7 days of culture. Only 1 sample (3.a) showed a 0.56-fold decrease in cell number on day 4, but by day 7 the number of the cells in this sample had increased. The observation of individual cultures (Figure 1) showed some heterogeneity among samples, particularly when TPO + FL was used. With this combination of growth factors, the ...
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Although recent studies have demonstrated the in vitro hepatic differentiation potential of mesenchymal stem cells (MSCs), the evidence supporting the in vivo engraftment of MSCs, hepatic differentiation and improvement of hepatic function is still lacking. We investigated in vivo hepatic differentiation potential and therapeutic effect of cord blood derived-MSCs (CBMSCs) transplantation in a cirrhotic rat model. CBMSCs (2x106) were infused in Wistar rats with thioacetamide-induced chronic liver injury. Biochemical markers, liver fibrosis and engraftment of CBMSCs were assessed. Infused CBMSCs were detected in the perivascular or fibrous region of the liver and did not acquire mature hepatic phenotypes. There was no difference in biochemical markers and in the area of liver fibrosis between the experimental and placebo groups. After infusion of CBMSCs in our experimental cirrhotic rat model we did not observe an improvement of liver function and liver fibrosis. Inversely, CBMSCs could have a ...
The two most widely used sources of hematopoietic stem cells for allogeneic transplants in pediatric practice are bone marrow BM and cord blood CB. While bone marrow transplantation BMT is reaching its 30th year of application, human umbilical cord blood transplantation HUCBT is approaching its 10th. Although these procedures have basically the...
Oxidative stress is associated with the development of various diseases including cancer, arteriosclerosis, diabetes mellitus, hypertension and metabolic syndrome. However, little is known about the involvement of 8-hydroxydeoxyguanosine (8-OHdG) dur
In this scholarly study, we established and characterized human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) from four different donors. of N-cadherin-mediated cellCcell get in touch with activated reductions of ERK account activation and led to VEGF downregulation. Furthermore, by analyzing hUCB-MSCs overexpressing N-cadherin or N-cadherin knockdown hUCB-MSCs, the function was confirmed by us of N-cadherin. In addition, we noticed that DiI-labeled hUCB-MSCs sole N-cadherin in the peri-infarct interact and area with cardiomyocytes. Launch Many preclinical research have got showed that control cells can improve cardiac function and promote angiogenesis after myocardial infarction (MI).1,2 However, latest individual studies have got shown conflicting outcomes.3,4,5 There are many potential reasons for such discrepancies, including differences among species, biology, disease models, and cell arrangements before delivery. Variants in control cells from specific sufferers may end ...
Bulgular: Otuz taze KK nitesinde her KK i in ortalama de erler: Toplam ekirdekli h cre say s (TNC): 93,8 30,1x107, CD34+: 3,85 2,55x106, ALDH+: 3,14 2,55 x106, CFU-GM: 2,64 1,96x105. On dokuz KK nitesinde donma zme sonras h cre de erleri: TNC: 32,79 17,27x107, CD34+: 2,18 3,17x106, ALDH+: 2,01 2,81x106, CFUGM: 0,74 0,92x105 dir. Bulgular m z; taze KK da TNC, CD34 ve ALDH; CFU-GM, CFU-GEMM ve BFU-E ile korelasyon g sterirken (TNC, r=0,47, r=0,35, r=0,41; CD34+, r=0,44, r=0,54 r=0,41; ve ALDH, r=0,63 r=0,45 r=0,6) donma zme sonras KK da korelasyon s ras yla CFU-GM, CFU-GEMM, ve BFU-E i in, TNC r=0,59, r=0,46, r=0,56, CD34+ r=0,67, r=0,48, r=0,61 ve ALDH r=0,61, r=0,67, r=0,67 olarak saptanm t r. B t n bulgular m z istatistiksel olarak anlaml km t r ...
Objective: Cyclosporin A (CsA), effective in prophylaxis and treatment of graft-vs-host disease (GVHD) after human allogeneic transplantation, blunts T-cell responses by inhibiting nuclear factor of activated T cells 1 (NFAT1) activation. This laboratory has shown that NFAT1 protein expression is severely reduced in human UCB (umbilical cord blood) T cells. Since UCB is increasingly used as a hematopoietic stem cell source in allogeneic transplantation, it is important to determine whether CsA sensitivity in UCB differs from that of adult T cells.,br /,,br /,Methods: Surface flow cytometric analysis, intracellular cytokine staining, flow cytometric analysis of cell death, and thymidine incorporation were used in this study to determine T-cell activation and effector functions during primary and secondary stimulation in the presence of CsA.,br /,,br /,Results: Although we observed differential CsA sensitivity of T-cell activation marker (CD69, CD45RO, CD25) upregulation comparing UCB and adult, ...
HACKENSACK, N.J., September 29, 2020 ( - Royal Biologics, an ortho-biologics company specializing in the research and advancement of novel ortho-biologics solutions, today announced the 100th successful implantation of Cryo-Cord™, cryopreserved placental umbilical cord.. They also announced that on Aug. 26 the first subject enrolled in their IRB approved multi-center pilot study to evaluate the efficacy​ of non-dimethyl​ sulfoxide (non-DMSO) viable umbilical cord graft on diabetic food ulcers was treated with Cyro-Cord™ cryopreserved placental umbilical cord graft by Dr. Robert Fridman of Foot Associates of New York.. A next-generation cryopreserved placental umbilical cord allograft, Cryo-Cord™ may be used as an anatomical barrier in numerous clinical applications.. Cryo-Cord™ enables providers with the first DMSO-free viable cell umbilical cord tissue. It is processed using aseptic techniques and frozen with a proprietary cryoprotectant.. Cryo-Cord™ has been obtained ...
Definition of Fetal Blood Sample in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is Fetal Blood Sample? Meaning of Fetal Blood Sample as a finance term. What does Fetal Blood Sample mean in finance?
With the goal of deriving clinically safe USSCs, we aimed to culture established USSCs in the serum- and animal component-free medium, USSC growth mediumACF. We observe that USSCs continue to proliferate in USSC growth mediumACF, but after one to three passages, the cells aggregate and grow in suspension as spheres. We show that spheres can be dissociated and can continue to grow for five passages, as long as they are dissociated before the sphere becomes cystic. We also show the spheres can revert to monolayer growth when provided with extracellular matrix support or when plated in medium containing fetal calf serum. Cells passaged in USCC growth mediumACF maintained their gene expression profile as judged by Sox2, Brachyury, Pax6 and Gata6 expression. Cells also maintained their capacity to form bone-like Alizarin red positive cells, SPC positive epithelial cells and β-tubulin III positive neuronal cells after directed differentiation. This is the first report of a serum-free medium that ...
TY - JOUR. T1 - Tumor necrosis factor-α is decreased in the umbilical cord plasma of patients with severe preeclampsia. AU - Kupferminc, Michael J.. AU - Peaceman, Alan M.. AU - Dollberg, Shaul. AU - Socol, Michael L.. PY - 1999/1/1. Y1 - 1999/1/1. N2 - We investigated the role of the fetal immune system in pregnancies complicated by preeclampsia by assessing umbilical cord plasma levels of the cytokines tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Nineteen nulliparous patients with severe preeclampsia composed the study group (group A). A comparison group was comprised of 19 healthy nulliparous patients with uneventful pregnancies (group B). Mixed umbilical cord blood was collected immediately after delivery. Plasma was prepared and all samples were assayed for TNF-α and IL-1β by specific enzyme-linked immunoassays (ELISAs). Data are presented as the median with range of values. The length of labor was similar in both groups. TNF-α was detected less frequently in the ...
Cord blood hematopoietic progenitor cells (CB-HPCs) transplanted immunodeficient NOD/LtsZ-scidIL2Rnull (NSG) and NOD/SCID/IL2Rnull (NOG) mice want efficient individual cell engraftment for long-term HIV-1 replication research. marker and microbial translocation after HIV-1 an infection. Humanized NSG mice reconstituted regarding to your brand-new process produced, moderate humoral and mobile immune system responses to HIV-1 postinfection. We think that NSG mice reconstituted regarding to your simple to use process will provide an improved in vivo model for HIV-1 replication and anti-HIV-1 therapy studies. Introduction Lately, efforts have already been designed to reconstitute an operating individual disease fighting capability in murine versions [1], [2]. Multilineage differentiation and self-renewal capability of Compact disc34+ cells have already been explored to reconstitute several sort of immunodeficient mice [3], [4], [5], [6], [7]. Third era, NOD-Rag1nullIL2Rnull, NOD/LtSz-scid/IL2Rnull ...
Autocrine Action of Thrombospondin-2 Determines the Chondrogenic Differentiation Potential and Suppresses Hypertrophic Maturation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells
Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Improve Neuropathology and Cognitive Impairment in an Alzheimers Disease Mouse Model Through Modulation of Neuroinflammation
title: Impaired function and epigenetic changes of human cord blood-derived CD133+/C-kit+Lin- endothelial progenitor cells in preeclampsia, doi: none, category: Thesis
This is a long term follow-up study to investigate the safety and efficacy of CARTISTEM, human umbilical cord blood-derived mesenchymal stem cells, in repair of
Although pharmacological methods for treating AD have been discovered, none significantly delay the progression of the disease. However, cell transplantation research using animals modeled with AD has indicated that human umbilical cord blood cells (HUCBCs) can ameliorate some cognitive deficits and reduce the effects of the amyloid-beta (Aβ) plaques, one of the physiological hallmarks of AD, comprised of peptides of 36-43 amino acids. However, the role that HUCBCs play in Aβ clearance has yet to be elucidated ...
TY - JOUR. T1 - Expression of the costimulator molecules, CD40 and CD154, on lymphocytes from neonates and young children. AU - Elliott, Salenna. AU - Roberton, Don M.. AU - Zola, Heddy. AU - MacArdle, Peter J.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - Differential expression of the costimulator molecules CD40 and CD154 on neonatal lymphocytes may be one explanation for limited T-dependent antibody responses in human neonates. CD40 was expressed at similar levels on resting B cells from adults, young children (2-20 months of age) or cord blood. CD40 expression was higher on cord blood B cells compared to adult B cells after stimulation with PMA and ionomycin, but similar on adult and cord blood B cells activated by CD3-stimulated T cells. In contrast to previous reports, cord blood T cells stimulated with PMA and ionomycin expressed adult levels of CD154 initially, but this expression was more transient on cord blood T cells. When adult and cord blood mononuclear cells were stimulated with CD3 mAb, T ...
Cell Transplantation publishes original, peer-reviewed research and review articles on the subject of cell transplantation and its application to human diseases. To ensure high-quality contributions from all areas of transplantation, separate section editors and editorial boards have been established. Articles deal with a wide range of topics including physiological, medical, preclinical, tissue engineering, and device-oriented aspects of transplantation of nervous system, endocrine, growth factor-secreting, bone marrow, epithelial, endothelial, and genetically engineered cells, among others. Basic clinical studies and immunological research papers are also featured. To provide complete coverage of this revolutionary field, Cell Transplantation will report on relevant technological advances, and ethical and regulatory considerations of cell transplants. Cell Transplantation is now an Open Access journal starting with volume 18 in 2009, and therefore there will be an inexpensive publication ...
Diabetes mellitus (DM) is a high risk factor for stroke and leads to more severe vascular and white-matter injury than stroke in non-DM. We tested the neurorestorative effects of delayed human umbilical cord blood cell (HUCBC) treatment of stroke in type-2 diabetes (T2DM). db/db-T2DM and db/+-non-DM mice were subjected to distal middle cerebral artery occlusion (dMCAo) and were treated 3 days after dMCAo with: (a) non-DM + Phosphate buffered saline (PBS); (b) T2DM + PBS; (c) T2DM + naïve-HUCBC; (d) T2DM + miR-126(-/-) HUCBC. Functional evaluation, vascular and white-matter changes, neuroinflammation, and miR-126 effects were measured in vivo and in vitro. T2DM mice exhibited significantly decreased serum and brain tissue miR-126 expression compared with non-DM mice. T2DM + HUCBC mice exhibited increased miR-126 expression, increased tight junction protein expression, axon/myelin, vascular density, and M2-macrophage polarization. However, decreased blood-brain barrier leakage, brain hemorrhage, and miR
TY - JOUR. T1 - Determination of engraftment potential of human cord blood stem-progenitor cells as a function of donor cell dosage and gestational age in the NOD/SCID mouse model. AU - Ural, Serdar. AU - Sammel, Mary D.. AU - Blakemore, Karin J.. PY - 2005/9/1. Y1 - 2005/9/1. N2 - Objective: The purpose of this study was to determine cell dosage parameters for successful engraftment of human cord blood hematopoietic stem cells (HSC) using an in vivo assay system, and to determine if there are differences with donor gestational age. Study design: HSCs were transplanted into nonobese diabetic-severe combined immunodeficient (NOD/SCID) mice. Donor cell dosage and gestational age ranges were 1 to 40×106 CD34+ cells per mouse, and 23 to 40 weeks, respectively. Recipient bone marrow was assessed for engraftment capacity of the HSCs. Results: There was increasing engraftment levels with increasing dosages of transplanted HSCs. When controlled for donor HSC dosage, engraftment levels using donor cord ...
Human umbilical cord blood derived CD34+ stem cells are reported to mediate therapeutic effects in stroke animal models. Estrogen was known to protect against ischemic injury. The present study wished to investigate whether the protective effect of CD34+ cells against ischemic injury can be reinforced with complemental estradiol treatment in female ovariectomized rat and its possible mechanism. Experiment 1 was to determine the best optimal timing of CD34+ cell treatment for the neuroprotective effect after 60-min middle cerebral artery occlusion (MCAO). Experiment 2 was to evaluate the adjuvant effect of 17β-estradiol on CD34+ cell neuroprotection after MCAO. Experiment 1 showed intravenous infusion with CD34+ cells before MCAO (pre-treatment) caused less infarction size than those infused after MCAO (post-treatment) on 7T magnetic resonance T2-weighted images. Experiment 2 revealed infarction size was most significantly reduced after CD34+ + estradiol pre-treatment. When compared with no treatment
TY - JOUR. T1 - The developmental potential and analysis of human fetal cord blood. AU - Cascio, Jeff Lo. AU - Boyse, Edward A.. AU - Bard, Judith. AU - Harris, David T.. PY - 1991/1/1. Y1 - 1991/1/1. UR - UR - U2 - 10.1016/0145-305X(91)90338-Y. DO - 10.1016/0145-305X(91)90338-Y. M3 - Article. AN - SCOPUS:27244462343. VL - 15. SP - S106. JO - Developmental and Comparative Immunology. JF - Developmental and Comparative Immunology. SN - 0145-305X. IS - SUPPL. 1. ER - ...
Umbilical cord blood-derived endothelial colony-forming cells (UCB-ECFC) show utility in neovascularization, but their contribution to osteogenesis has not been defined. Cocultures of UCB-ECFC with human fetal-mesenchymal stem cells (hfMSC) resulted in earlier induction of alkaline phosphatase (ALP) (Day 7 vs. 10) and increased mineralization (1.9x; p < .001) compared to hfMSC monocultures. This effect was mediated through soluble factors in ECFC-conditioned media, leading to 1.8-2.2x higher ALP levels and a 1.4-1.5x increase in calcium deposition (p < .01) in a dose-dependent manner. Transcriptomic and protein array studies demonstrated high basal levels of osteogenic (BMPs and TGF-βs) and angiogenic (VEGF and angiopoietins) regulators. Comparison of defined UCB and adult peripheral blood ECFC showed higher osteogenic and angiogenic gene expression in UCB-ECFC. Subcutaneous implantation of UCB-ECFC with hfMSC in immunodeficient mice resulted in the formation of chimeric human vessels, ...
Kim DS, Kim JH, Lee JK, Choi SJ, Kim JS, Jeun SS, Oh W, Yang YS, Chang JW. Overexpression of CXC chemokine receptors is required for the superior glioma-tracking property of umbilical cord blood-derived mesenchymal stem cells. Stem Cells Dev. 2009 Apr; 18(3):511-9 ...
There are several advantages of using umbilical cord blood stem cells over bone marrow stem cells for transplants (see Table 2). The first advantage is that umbilical cord blood is relatively easy to collect and process. Once considered a substance to be thrown away after a birth, now the cord blood can be easily saved. After it is saved and sent to a storage facility, the cord blood is quickly available for use within days to weeks after processing. In contrast, bone marrow stem cells can take much longer to find a match, collect the sample, and process. The process for bone marrow transplantation can take from weeks to months. The collection process for cord blood is not painful to either mother or child and can be done either prior to or after the delivery of the placenta (Gonzalez-Ryan, VanSyckle, Coyne, & Glover, 2000; Percer, 2009). Bone marrow transplants, on the other hand, require the donor to be hospitalized, anesthetized, and experience postcollection pain and discomfort. Thus, ...
Dendritic cells (DC) generated from human umbilical cord blood might replace patients' DC in attempts to elicit tumor-specific immune response in cancer patients. We studied the efficiency of transfection of human cord blood DC with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene, to test if nonviral gene transfer would be a method to load DC with protein antigens for immunotherapy purposes. Cord blood mononuclear cells were cultured in serum-free medium in the presence of granulocyte-monocyte colony stimulating factor (GM-CSF), stem cell factor (SCF) and Flt-3 ligand (FL), to generate DC from their precursors, and thereafter transfected by electroporation. Maturation of DC was induced by stimulation with GM-CSF, SCF, FL and phorbol myristate acetate (PMA). Transfected DC strongly expressed EGFP, but transfection efficiency of DC, defined as HLA-DR+ cells lacking lineage-specific markers, did not exceed 2.5%. Expression of the reporter gene ...
Hematopoiesis depends on the association of hematopoietic stem cells with stromal cells that constitute the hematopoietic microenvironment. The in vitro development of the endothelial cell from umbilical cord blood (UCB) is not well established and has met very limited success. In this study, UCB CD34(+) cells were cultured for 5 weeks in a stroma-free liquid culture system using thrombopoietin, flt3 ligand, and granulocyte-colony stimulating factor. By week 4-5, we found that firmly adherent fibroblast-like cells were established. These cells showed characteristics of endothelial cells expressing von Willebrand factor, human vascular cell adhesion molecule-1, human intracellular adhesion molecule-1, human CD31, E-selectin, and human macrophage. Furthermore, when comparing an ex vivo system without an established endothelial monolayer to an ex vivo system with an established endothelial monolayer, better expansion of total nucleated cells, CD34(+) cells, and colony-forming units ...
This is an open-label, delayed-treatment trial.. A total of 12 subjects fulfill the inclusion and exclusion criteria will be recruited and randomly assigned into two treatment group. Group A (early-treatment group) will receive transplant of UCBMC isolated from HLA-matched umbilical cord blood at Day 0. Group B (delayed-treatment group) will participate in 6 months observation before the UCBMC transplantation at Month 6. All subjects will be followed up for 18 months from enrollment at Day 0. Long-term follow-up will be carried up to 36 months if applicable.. The adverse events and safety parameters will be collected and recorded. In addition, the stroke scores , gait and brain MRI will be obtained before and after the treatment to assess the safety and potential treatment effect of UCBMC in chronic ischemic stroke. ...
This is an open-label, delayed-treatment trial.. A total of 12 subjects fulfill the inclusion and exclusion criteria will be recruited and randomly assigned into two treatment group. Group A (early-treatment group) will receive transplant of UCBMC isolated from HLA-matched umbilical cord blood at Day 0. Group B (delayed-treatment group) will participate in 6 months observation before the UCBMC transplantation at Month 6. All subjects will be followed up for 18 months from enrollment at Day 0. Long-term follow-up will be carried up to 36 months if applicable.. The adverse events and safety parameters will be collected and recorded. In addition, the stroke scores , gait and brain MRI will be obtained before and after the treatment to assess the safety and potential treatment effect of UCBMC in chronic ischemic stroke.. ...
We conducted over 1260 cord blood transfusions in consented volunteers with anemic (Hemoglobin less than 8gm/100ml) from 1999 till date in children an..
Deficient CD40 ligand (CD40L) expression by neonatal T cells has been proposed to explain the diminished Ig production by newborns. Therefore, we examined the expression and function of CD40L on activated neonatal CD4+ T cells isolated from cord blood. Anti-CD3 activation of neonatal T cells resulted in the expression of CD40L demonstrated with specific mAb or a soluble CD40.G1 construct. The level of expression was comparable to that of adult memory CD4+ T cells. In contrast, PMA and ionomycin induced only minimal CD40L expression by neonatal T cells, whereas adult memory T cells expressed CD40L comparably after stimulation with PMA and ionomycin or anti-CD3. The expression of CD40L was blocked by cyclosporine and prostaglandin E2 (PGE2). IL-2 IL-4 partially reversed the inhibition of CD40L expression by cyclosporine, and IL-2 reversed the inhibition by PGE2. CD40L expressed by neonatal T cells was functionally active, since neonatal T cell-dependent production of IgG4 and IgE was inhibited by ...
Seven cord blood (CB) units were tested for their capacity to repopulate irradiated NOD/SCID mice after one or two successive cryopreservation procedures. In primary transplants with frozen or refrozen CB cells we observed equivalent human colonies and percentages of human CD45+ cells, with multilineage engraftment. In secondary transplants flow cytometry and polymerase chain reaction for the a satellite region of chromosome 17 showed equivalent levels of human engraftment. Since CB units have, to date, mainly been stored in individual bags, our results suggest new options for optimizing the timing of infusions of expanded and non-expanded progenitors in transplants ...
De Wynter, E.A., Buck, D., Hart, C., Heywood, R., Coutinho, L.H., Clayton, A., Rafferty, J.A., Burt, D., Guenechea, G., Bueren, J.A., Gagen, D., Fairbairn, L.J., Lord, B.I. & Testa, N.G. (1998) CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. Stem Cells, 16, 387-396. ...
BACKGROUND: Human umbilical cord blood (HUCB) is a possible alternative to bone marrow (BM) and mobilized peripheral blood (PB) for transplantation of hematopoietic progenitors. The aim of this study was to evaluate the phenotypic profile of CD34+ progenitors present in HUCB. MATERIALS AND METHODS: A flow cytometric analysis was performed on 20 HUCB samples, using a large panel of monoclonal antibodies recognizing different lineage or activation antigens, in double labeling with CD34. RESULTS: A toal of 13,897 +/- 2,529 cells/microL, 0.84 +/- 0.83% of which were CD34+, was found. The large majority of CD34+ cells were committed toward initial myeloid differentiation (CD33+, CD13+) and expressed the transferrin receptor (CD71). A substantial proportion of these cells (about 40%) co-expressed CD45RA and CD117, while a very small number displayed markers of advanced myeloid commitment, such as CD14, CD15 and CD41 (less than 2%), or those of lymphoid differentiation: CD2, CD5, CD7, CD10 and CD19 ...
A Ph.D. student of mine had purchased a reagent she needed for her cell culture work on her thesis. The reagent was not high-tech or anything sensitive of that nature. I do not know whether anyone is interested, but she is working on a very novel topic ie; to study the effect of certain neurotransmitters on cord blood cells, which are now highly regarded in transplantation studies. She had ordered the reagent from a local company who dealt with an American producer on the other side. When she had received the reagent she found that the production label and lot number did not match with the company information provided ...
TY - JOUR. T1 - Retention of stemness and vasculogenic potential of human umbilical cord blood stem cells after repeated expansions on PES-nanofiber matrices. AU - Joseph, Matthew. AU - Das, Manjusri. AU - Kanji, Suman. AU - Lu, Jingwei. AU - Aggarwal, Reeva. AU - Chakroborty, Debanjan. AU - Sarkar, Chandrani. AU - Yu, Hongmei. AU - Mao, Hai Quan. AU - Basu, Sujit. AU - Pompili, Vincent J.. AU - Das, Hiranmoy. PY - 2014/10. Y1 - 2014/10. N2 - Despite recent advances in cardiovascular medicine, ischemic diseases remain a major cause of morbidity and mortality. Although stem cell-based therapies for the treatment of ischemic diseases show great promise, limited availability of biologically functional stem cells mired the application of stem cell-based therapies. Previously, we reported a PES-nanofiber based ex vivo stem cell expansion technology, which supports expansion of human umbilical cord blood (UCB)-derived CD133+/CD34+ progenitor cells ~225 fold. Herein, we show that using similar ...
Unrelated umbilical cord blood stem cell transplant after failure of haploidentical or matched unrelated donor hematopoietic stem cell transplant.
Objective The aim of the study was to evaluate the efficacy and safety of umbilical cord blood stem cells (USCs) transplantation combined with routine supportive therapy (RST) for liver cirrhosis (LC). Materials and methods Clinical trials involved in this research were searched from Web of Science, PubMed, EMBASE, Cochrane Library, Wanfang and CNKI database. Treatment effects, quality of life (QoL), adverse events and other outcome measures were extracted and evaluated. Results A total of 10 trials including 616 LC patients were involved in this study. Based on our analysis, the liver function of LC patients was significantly improved after USCs transplantation and RST combined therapy, indicated by decreased total bilirubin, alanine aminotransferase, aspartate aminotransferase levels and prothrombin time and increased serum albumin level and prothrombin activity. Compared to those treated by RST alone, patients treated by combined therapy
The latest market report published by Credence Research, Inc. Global Umbilical Cord Blood Banking Market - Growth, Share, Opportunities, Competitive Analysis, and Forecast, 2017 - 2025, the umbilical cord blood banking market was valued at USD 2,875.2 Mn in 2016, and is expected to reach USD 7,623.3 Mn by 2025, expanding at a CAGR of 11.3% from 2017 to 2025.. Browse the full report Umbilical Cord Blood Banking Market - Growth, Share, Opportunities, Competitive Analysis, and Forecast, 2017 - 2025 report at Market Insights. Recently, there has been several changes in the cord blood industry with a few key trends such as diversification of storage services, partnering with prenatal or neonatal or maternal health services, industry consolidation through acquisitions, and focus on cord blood cell expansion. The several diversified services of cord blood banks comprises storage of umbilical cord tissue (Whartons Jelly), ...
The latest market report published by Credence Research, Inc. Global Umbilical Cord Blood Banking Market - Growth, Share, Opportunities, Competitive Analysis, and Forecast, 2017 - 2025, the umbilical cord blood banking market was valued at USD 2,875.2 Mn in 2016, and is expected to reach USD 7,623.3 Mn by 2025, expanding at a CAGR of 11.3% from 2017 to 2025.. Browse the full report Umbilical Cord Blood Banking Market - Growth, Share, Opportunities, Competitive Analysis, and Forecast, 2017 - 2025 report at Market Insights. Recently, there has been several changes in the cord blood industry with a few key trends such as diversification of storage services, partnering with prenatal or neonatal or maternal health services, industry consolidation through acquisitions, and focus on cord blood cell expansion. The several diversified services of cord blood banks comprises storage of umbilical cord tissue (Whartons Jelly), ...
The main purpose of this investigational (not approved by the FDA) Phase I research is to test whether transplantation of umbilical cord blood cells can
Umbilical cord of a newborn baby can save lives to others who has this illness. Did you know that Cryo-Cell International, Inc. is the largest and fastest growing U-Cord stem cell banking firm? If you want to save lives, why not Bank your babys cord blood with Americas most established family cord blood bank? You can help many lives if you bank the umbilical cord of your newborn baby at Cryo-Cell International. They are the Innovative Stem Cell Solutions. They offers innovative and safe solutions for cord blood banking. They are dedicated to educating expectant parents about the benefits of preserving their newborns from cord blood stem cells banking. There are over 155,000 families preserve their newborns umbilical cord blood for potential use against many diseases. Mostly pregnancy women are those who are willing to bank the umbilical cord of their newborn baby. Are you one of them? If you want to make it sure that the umbilical cord is safe to your loveones then you can compare cord blood ...
A cord blood bank is a facility which stores umbilical cord blood for future use. Both private and public cord blood banks have developed in response to the potential for cord blood in treating diseases of the blood and immune systems. Public cord blood banks accept donations to be used for anyone in need, and as such function like public blood banks. Traditionally, public cord blood banking has been more widely accepted by the medical community. Private cord blood banks store cord blood solely for potential use by the donor or donors family. Private banks typically charge around $2,000 for the collection and around $200 a year for storage. The policy of the American Academy of Pediatrics states that private storage of cord blood as biological insurance is unwise unless there is a family member with a current or potential need to undergo a stem cell transplantation. The American Academy of Pediatrics also notes that the odds of using ones own cord blood is 1 in 200,000 while the Institute ...
Tens of thousands of patients need bone marrow transplants (BMT) every year, some for bone marrow (BM) cancers and some for inherited diseases such as sickle cell anemia and thalassemia, but many lack a BM donor. African Americans, Asian Americans, and people of Hispanic descent are more likely than others to lack a stem cell donor. BMTs provide hematopoietic (blood) stem and progenitor cells (HS/PCs) that replace the patients diseased BM with healthy BM. The new BM provides all the circulating blood cells throughout life. Many BMTs use HS/PCs that do not come from the BM. One such other source is umbilical cord blood (UCB). UCB HS/PCs have many advantages over other HS/PC sources (i.e., BM or peripheral blood). For example, we can easily obtain UCB HS/PCs without any risk to the donor, and we can keep the cells stored in freezers to be available when a patient needs them. However, most UCB samples contain too few HS/PCs to be used to treat people. Expanding the number of HS/PCs in UCB ...
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Background: Measures were introduced in both the House and Senate to establish a National Cord Blood Stem Cell Bank Network.. Umbilical cord blood stem cells are obtained from the blood contained in the delivered placenta and umbilical cord, which are normally discarded after childbirth. Obtaining these stem cells presents no inherent moral concerns. Through freezing they can be preserved for many years.. According to findings presented in the House measure, cord blood stem cell transplants can be used for bone marrow reconstitution to treat malignancies such as leukemia and lymphoma, genetic disorders such as sickle cell anemia, and acquired diseases. The findings also claim that cord blood stem cells do not have to be matched as closely as bone marrow transplants. This means patients will be more likely to find a suitable unrelated cord blood donor than a matched bone marrow donor. Supporters say a network of at least 150,000 units of ethnically balanced cord blood donors would provide ...
The University of Colorado Cord Blood Bank (UCCBB), a major component of ClinImmune Labs, has a seventeen year history of collecting, processing, banking and distributing cord blood for human transplantation. UCCBB is a public cord blood bank, which has consented 30,000 women and banked over 8,500 cord blood units, of which more than 770 have been transplanted at over 150 different transplant centers in the United States and abroad. UCCBB is a major provider of Hispanic cord blood units to patients in the United States, Mexico, and Central and South America. UCCBB is accredited by AABB and licensed by the FDA (May 24, 2012 Approval Letter-HPC, Cord Blood). The Health Resources and Services Administration (HRSA) awarded UCCBB a cord blood collection contract beginning in 2006. UCCBB provides cord blood to stem cell investigators in Michigan, New York, Tennessee, Washington, and Colorado. The bank is managed by immunogeneticists and stem cell transplant physicians with decades of experience in ...
In a new peer-reviewed article published by the Journal of Translational Medicine, scientists from Beike Biotechnology,Chinas leading stem cell research and regenerative medicine company, and Medistem, Inc.,reported positive safety data in 114 patients who were treated by doctors at Nanshan Affiliated Hospital of Guangdong Medical College (Shenzhen Nanshan Hospital) in Shenzhen using Beikes proprietary cord blood stem cell transplantation protocol.
ATLANTA —Twelve percent of adults with acute myeloid or acute lymphoblastic leukemia who received umbilical cord blood transplantation experienced graft failure, according to a large European retrospective study presented at the ASH Annual Meeting and Exposition.“Our aim was to assess the outcomes of patients with acute leukemia who experience graft failure after umbilical cord blood
0054] In important embodiments of the invention, the umbilical cord blood cells are fractionated in order to generate enriched cell populations. As used herein, an enriched cell population is a cell population that has been manipulated in order to increase the frequency of a particular cell type in the population relative to the frequency of that cell type prior to manipulation. It is to be understood that the cell type being enriched is one that existed in the population prior to manipulation, and that enrichment results from the removal of other cell types from the population rather than addition of the cell type of interest. Of particular interest according to the invention are cell populations enriched in CD34+, CD133+, or CD34+/CD133+ cells. CD34 and CD133 are cell surface protein (or markers) that have been identified previously as present on hematopoietic progenitor cells (including on hematopoietic stem cells). As used herein, a CD34+ cell is a cell that expresses CD34 on its cell ...
TY - JOUR. T1 - Polychromatic flow cytometry analysis of CD34+ hematopoietic stem cells in cryopreserved early preterm human cord blood samples. AU - D'Alessio, F.. AU - Mirabelli, P.. AU - Gorrese, M.. AU - Scalia, G.. AU - Gemei, M.. AU - Mariotti, E.. AU - Di Noto, R.. AU - Martinelli, P.. AU - Fortunato, G.. AU - Paladini, D.. AU - Del Vecchio, L.. PY - 2011/1. Y1 - 2011/1. N2 - During the last decades, extended characterizations were performed of human full-term cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. First, we observed that the percentage of CD34 PosCD45 Dim cells was higher in hEPCB compared with hTCB and that it decreased during 16th-20th week of pregnancy. Within the CD34 PosCD45 Dim population, we examined the expression of CD29, CD31, CD38, CD90, CD117, CD133, CD135, CD200, CD243, and CD338. We found that CD135 ...
Wise presentation at is the first verified CNS nerve regeneration info weve seen. His procedure being: 1. filter out mononuclear cells from HLA-matched umibilical cord blood 2. open the spinal cord dura 3. inject the mononuclear cells 3mm into the dorsal root above and below the injury site 4. use lithium carbonate (maybe/maybe not) to stimulate nerve regrowth - still trialling this plus other grow factors 5. wait for nerves to
The authors induced SCI in 10 dogs by percutaneous balloon compression. The 10 injured dogs were assigned randomly to the following groups (2 dogs each): Group 1, evaluated 2 weeks after sham transplantation; Group 2, evaluated 2 weeks after transplantation; Group 3, evaluated 4 weeks after sham transplantation; Group 4, evaluated 4 weeks after transplantation; and Group 5, evaluated 4 weeks after multispot transplantations. The dogs with sham transplantation (Groups 1 and 3) received the same volume of saline, as a control. A spinal needle was advanced into the spinal canal, and the investigators confirmed that the end of the spinal needle was located in the ventral part of spinal cord parenchyma by using contrast medium under fluoroscopic guidance. The hUCB-derived MSCs were transplanted into the cranial end of the injured segment in 6 injured dogs at 7 days after SCI. ...
Although cord blood is currently considered discarded human material, it should only be collected for banking with an institutional review board-approved protocol and with signed informed consent from a parent.42,43 Pertinent donor information communicated to the cord blood bank should be kept confidential by the cord blood bank and used only to report important medical information obtained during the cord blood collection, processing, and screening process that is relevant to the safety of the donor and family. If cord blood was collected from a newborn who subsequently developed a genetic, immunologic, or malignant neoplastic disorder, parents should notify the cord blood bank so that the unit is not used for transplantation. All cord blood units banked for potential use should be tested for infectious diseases, similar to those tested in a blood bank, and for hereditary hematologic diseases. The informed consent must contain information pertaining to what tests are to be performed on the cord ...
There was nothing unusual about the birth of a baby girl in Sacramento one Friday afternoon late in October, 2012. This birth was special, however, because just a few hours later both Priscilla Williams (BME & DEB graduate student) and Eduardo Silva (BME & DEB Assistant Professor) were busy isolating stem cells from the babys umbilical cord blood. The Silva Lab is one of the first recipients of cord blood from the recently established Umbilical Cord Blood Collection Program.. The Silva Labs current project will isolate vascular progenitor cells from the cord blood with the premise that they can investigate and develop new strategies to actively direct their homing in vivo. In the near future, Silvas group will isolate not only vascular progenitor cells but also other progenitor and stem cell populations. For example, in the next couple of months the Silva Lab and Scott Simons Lab will collaborate to develop strategies to isolate hematopoietic stem and progenitor cells (HSPCs) and ...
To determine if overweight/obese pregnant women have altered microRNA expression patterns in fetal umbilical cord blood that may affect the development of offspring. Umbilical cord blood samples were obtained from the fetuses of 34 overweight/obese and 32 normal-weight women after delivery. Next generation sequencing (NGS) analyzed their miRNA expression patterns. miRanda and TargetScan databases were used to predict the miRNAs target genes followed by analyses of Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to perform function grouping and pathway analyses. qRT-PCR verified the identity of differentially expressed miRNAs that were revealed in the NGS results. There was a positive correlation between newborn body weight and pregestational BMI of pregnant individuals (r = 0.48, P | 0.001). One hundred and eight miRNAs were differentially expressed between the normal and overweight/obese groups, which target genes were enriched in the metabolic pathway. Five C19MC miRNAs (miR
There are options if you want to donate or save your babys cord blood:. 1. Donate your babys cord blood to a public bank, where it is made available to others, much like blood banks. Donating to a public cord blood bank should not cost you any money. Ask your provider if there will be any charge to collect the blood. 2. Save your babys cord blood in a private or family cord blood bank where it will be available to your baby and family members. Storing the cord blood in a private bank for future use by your baby or family usually costs between $1,000 to $2,000 when your baby is born. A yearly storage fee is approximately $100 to $150, and private banks do not charge a fee for the release of the cord blood to your family. There are some family banking programs available for free for those who have a sibling or family member with a condition treated by stem cells.. How do families decide if they want to save or donate cord blood? ...
Cord blood is obtained after the umbilical cord has been cut. The blood is frozen and can be stored in cord blood banks either privately or be donated to public cord blood banks.
Intrauterine exposure to gestational diabetes mellitus (GDM) confers a lifelong increased risk for metabolic and other complex disorders to the offspring. GDM-induced epigenetic modifications modulating gene regulation and persisting into later life are generally assumed to mediate these elevated disease susceptibilities. To identify candidate genes for fetal programming, we compared genome-wide methylation patterns of fetal cord bloods (FCBs) from GDM and control pregnancies. Using Illuminas 450K methylation arrays and following correction for multiple testing, 65 CpG sites (52 associated with genes) displayed significant methylation differences between GDM and control samples. Four candidate genes, ATP5A1, MFAP4, PRKCH, and SLC17A4, from our methylation screen and one, HIF3A, from the literature were validated by bisulfite pyrosequencing. The effects remained significant after adjustment for the confounding factors maternal BMI, gestational week, and fetal sex in a multivariate regression model. In
BACKGROUND: Because there are lower incidence of graft versus host disease in HLA mismatched cord blood transplantation compared to bone marrow transplantation, development of smaller scale cord blood bank could be possible. So we analysed the content of hematopoietic stem cell in cord blood and the distribution of HLA as a basic study for cord blood bank. METHODS: Seventy eight cord bloods were collected in heparinized bottle immediately after caesarian section. After expulsion of placenta, additional cord blood and placental blood were collected with heparinized syringe. Fifteen mL was sent to the laboratory for analysis and the rest was cryopreserved. RESULTS: The mean collected cord blood volume was 96.8mL (range, 55~163mL). And mean 81.8mL (range, 40~148mL) was cryopreserved. It contained mean 7.4x108 (range, 2.8x108~12.2x108) nucleated cells. In 2x105 mononuclear cells, 85 +/- 48 BFU-E, 19 +/- 17 CFU-E, 107 +/- 73 CFU-GM and 124 +/- 113 CFU-GEMM were present. With dextran/albumin thawing ...
The use of cord blood cells as hematopoietic stem cell grafts for patients with hematologic malignancies receiving an allogeneic stem cell transplant has been limited to children due to the small number of stem cells present in a single cord blood collection. The result of these limitations has been a high rate of graft failures in adult patients.. To overcome these limitations, researchers from the Icahn School of Medicine at Mount Sinai in New York City treated CD34-positivecells with a combination of histone deacetylase (HDAC) inhibitors and valproic acid. They found that the treated cells produced a greater number of repopulating cells, and established multilineage hematopoiesis in primary, secondary, and tertiary immune-deficient mice. The findings may provide clinical benefit for adults with leukemia, lymphoma, and other blood cancers. The study is published in The Journal of Clinical Investigation.. Study Methods and Results. The researchers treated dividing cord blood CD34-positive cells ...
Consider the following example. Many cellular therapeutic laboratories measure viability by staining cells with 7-AAD and flow cytometry. This method is used because, in hematopoietic stem cell processing laboratories, viability can be easily combined with other cell membrane markers, such as CD34 and CD45 in a single flow cytometric enumeration panel. This type of viability testing is regularly used for bone marrow, mobilized peripheral blood and umbilical cord blood stem cell products. Virtually all cord blood products are cryopreserved and can remain in the frozen state for many years. In the example shown in the accompanying graph, 56 cryopreserved cord blood samples were thawed, the mononuclear cells (MNC) fractionated by density gradient centrifugation and tested for viability using 7-AAD as well as measuring the proliferation, cellular functionality and viability using HALO®-96 SPC-QC. The discrepancy between dye exclusion and metabolic viability methodologies is readily apparent. In ...
Umbilical Cord Blood is the leftover blood in the umbilical cord and the placenta after the delivery of a baby and the cutting of the cord. Cord blood is generally discarded along with the placenta and the umbilical cord. When the fetal develops, the umbilical cord blood banking hails from the similar zygote as that of the human fetus comprising two umbilical arteries and one umbilical vein, concealed within Whartons Jelly. The fetus, oxygen and nutrient blood is supplied by the umbilical from the placenta. On the contrary, in case of the umbilical artery, the blood oxygen comes from nutrients.. After the successful 10 years of over 1500 stem cell transplantations, the release of stem cells from the umbilical cord blood appears nothing less than a miraculous drug for treating many dangerous diseases. Cord blood constitutes a majority of stem cells and thus, posing as a life-saving amazing technique for your baby and your family. In effect, all mothers and around half of their siblings are ...
Our data shows that EPCR represents a novel robust marker for expanded human LT-HSCs. In UM171-treated cultures, positivity for EPCR defines a highly HSC-enriched population, which includes most repopulating units. EPCR+ cells are uniformly positive for CD34, CD90, and CD133 and represent a subset of this triple positive population. EPCR is thus the most reliable indication of expanded human CB HSCs, especially in the presence of UM171. The utility of this marker to detect expanded HSCs is less obvious when using fresh CB cells because EPCR levels are lower in these cells (supplemental Figure 15; supplemental Table 6). Nonetheless, nonexpanded CB CD34+EPCR+ cells express HSC surface markers, such as CD90, CD49f, CD133, and ckit (supplemental Figure 16), and can repopulate secondary recipient mice (supplemental Figure 15), suggesting that EPCR also identifies LT-HSCs under these conditions. Using the EPCR marker as a tool to monitor ex vivo HSPC activity, we determined that EPCR-positive cells ...
ICD-10-PCS code 30233X0 for Transfusion of Autologous Cord Blood Stem Cells into Peripheral Vein, Percutaneous Approach is a medical classification as listed by CMS under Circulatory range.
Cord blood stem cell application is increasing a faster rate than other stem cell techniques because of its efficient and cost effective treatment p
... is a technique used in obstetrics during labor to confirm whether fetal oxygenation is sufficient. ... Analysis of lactate only requires 5 μl of blood. Fetal scalp stimulation test "Fetal scalp pH testing: MedlinePlus Medical ... "Determination of pH or lactate in fetal scalp blood in management of intrapartum fetal distress: Randomised controlled ... The procedure can be performed by creating a shallow cut by a transvaginally inserted blood lancet, followed by applying a thin ...
It can be used as a non-invasive alternative to fetal scalp blood testing. A firm digital pressure on head or a gentle pinch of ... An acceleration of the fetal heart rate of 15 bpm lasting at least 15 seconds is suggestive of normal fetal outcome. Fetal ... Fetal scalp stimulation test is a diagnostic test used to detect fetal metabolic acidemia. ... a clinical alternative to fetal scalp blood sampling". American Journal of Obstetrics and Gynecology. 148 (3): 274-7. doi: ...
The chorionic membrane is a fibrous tissue layer containing the fetal blood vessels. Chorionic villi form on the outer surface ... The fetal membranes separate maternal tissue from fetal tissue at a basic mechanical level. The fetal membrane is composed of a ... The fetal membranes surround the developing embryo and form the fetal-maternal interface. The fetal membranes are derived from ... Pathophysiology of the fetal membranes, such as microfractures, senescence of cells in the fetal membrane and inflammation can ...
... is the loss of fetal blood cells into the maternal circulation. It takes place in normal pregnancies ... from the red blood cells. Subsequent staining, using Shepard's method, makes fetal cells (containing foetal hemoglobin) appear ... Fetal-maternal haemorrhage is one cause of intrauterine death (IUD). The Kleihauer-Betke test is a blood test used to measure ... It is estimated that less than 1ml of fetal blood is lost to the maternal circulation during normal labour in around 96% of ...
... (cffDNA) is fetal DNA that circulates freely in the maternal blood. Maternal blood is sampled by ... Noninvasive Prenatal Diagnosis of Fetal Aneuploidy Using Cell-Free Fetal Nucleic Acids in Maternal Blood: Clinical Policy ( ... Approximately 11 to 13.4 percent of the cell-free DNA in maternal blood is of fetal origin. The amount varies widely from one ... Chiu RW, Poon LL, Lau TK, Leung TN, Wong EM, Lo YM (September 2001). "Effects of blood-processing protocols on fetal and total ...
"Animal welfare and ethics in the collection of fetal blood for the production of fetal bovine serum". Altex. 38 (2): 319-323. ... Fetal bovine serum (FBS) is derived from the blood drawn from a bovine fetus via a closed system of collection at the ... The blood is collected aseptically into a sterile container or blood bag and then allowed to clot. The normal method of ... Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to ...
Anderson, Ian (24 September 1987). Fetal fragments suggest warm-blooded dinosaurs. New Scientist. p. 25. ISSN 0262-4079. John ...
"Ultrasonic measurement of human fetal blood flow". Journal of Biomedical Engineering. 4 (1): 28-36. doi:10.1016/0141-5425(82) ...
If a pregnant woman is known to have anti-D antibodies, the Rh blood type of a fetus can be tested by analysis of fetal DNA in ... A blood type (also known as a blood group) is a classification of blood, based on the presence and absence of antibodies and ... A complete blood type would describe each of the 43 blood groups, and an individual's blood type is one of many possible ... Blood donors with exceptionally strong anti-A, anti-B or any atypical blood group antibody may be excluded from blood donation ...
Fielder, F.D.; Pocock, Pamela (1968). "Foetal blood flow detector". Ultrasonics. 6 (4): 240-241. doi:10.1016/0041-624X(68)90134 ... "Electronic foetal monitoring system, United Kingdom, 1980". Science Museum. Retrieved 2019-10-09. Brown, Tom. "An except from ... "Ultrasonic foetal heart monitor, England, 1973-1978". Science Museum. Retrieved 2019-10-09. Health and Social Service Journal, ... "Image of foetal monitoring system, united kingdom, 1980". Science & Society Picture Library. 2008-04-23. Retrieved 2019-10-09 ...
... maternal and fetal blood lead levels are virtually identical. Once in the fetal circulation, lead readily enters the developing ... Measuring a person's blood lead level requires a blood sample, which may be performed with a fingerstick or a blood draw. The ... Blood lead level (BLL), is a measure of the amount of lead in the blood. Lead is a toxic heavy metal and can cause neurological ... "since no safe blood lead level in children has been identified, a blood lead 'level of concern' cannot be used to define ...
Conventional methods for growing animal tissue in culture involves the use of fetal bovine serum (FBS). FBS is a blood product ... Nowak-Imialek, Monika; Niemann, Heiner (2016). Embryonic Stem Cells and Fetal Developmental Models. Fetal Stem Cells in ... Sothic Bioscience is building a platform for biosynthetic horseshoe crab blood production. Horseshoe crab blood contains ... "Blood, brains and burgers: The future is lab-grown everything". New Atlas. 2021-08-11. Retrieved 2021-08-15. "Under Development ...
Hennessy DP, Coghlan JP, Hardy KJ, Scoggins BA, Wintour EM (October 1982). "The origin of cortisol in the blood of fetal sheep ... and although lamb fetal cortisol is mostly of maternal origin during the first 122 days, 88% or more is of fetal origin by day ... increased fetal production of cortisol between weeks 30 and 32 initiates production of fetal lung pulmonary surfactant to ... It is released with a diurnal cycle and its release is increased in response to stress and low blood-glucose concentration. It ...
ISBN 3-540-58961-9. Ervin MG, Leake RD, Ross MG, Calvario GC, Fisher DA (May 1985). "Arginine vasotocin in ovine fetal blood, ... and possibly in mammals during the fetal stage of development. Arginine vasotocin (AVT), a hormone produced by neurosecretory ...
... blood plasma and switched strategy to search for cell-free fetal DNA in mother's blood. Lo, who by the time was married, ... using fetal DNA from fetal cells in the mother's blood. This, however, was stymied by a number of factors, including low ... His research focuses on the detection of cell-free fetal DNA in blood plasma. Lo was born in Hong Kong in 1963. His mother ... He then wondered if fetal DNA was detectable in mother's blood. Using PCR, he detected the Y chromosome in a mother bearing a ...
Moreover, neonates have much higher levels of bilirubin in their blood due to: Rapid breakdown of fetal red blood cells ... This breakdown of fetal red blood cells releases large amounts of bilirubin. Severe hemolytic disease of the newborn. Many ... The blood-brain barrier is not fully functional in neonates and therefore bilirubin is able to cross into the central nervous ... liver is heavily exercised by the breakdown of fetal hemoglobin as it is replaced with adult hemoglobin and the blood-brain ...
She was lying in the fetal position in a pool of blood. An autopsy determined that Brown had been stabbed seven times in the ...
"Fetal cells in maternal blood for prenatal diagnosis: a love story rekindled". Biomarkers in Medicine. 11 (9): 705-710. doi: ... For example, isolation of protoporphyrin IX from blood samples can be used as a diagnostic tool for atherosclerosis. Cancer ... "Liquid Biopsy: Using DNA in Blood to Detect, Track, and Treat Cancer". National Cancer Institute. 8 November 2017. Retrieved 12 ... primarily blood. Like traditional biopsy, this type of technique is mainly used as a diagnostic and monitoring tool for ...
... which is essential for fetal blood circulation. At birth, when the first breath is taken fetal blood flow is reversed to travel ... Within the fetal right atrium, blood from the inferior vena cava and the superior vena cava flow in separate streams to ... During atrial systole, blood not only empties from the atria to the ventricles, but blood continues to flow uninterrupted from ... entry hall') is one of two upper chambers in the heart that receives blood from the circulatory system. The blood in the atria ...
"Noninvasive blood tests for fetal development predict gestational age and preterm delivery". Science. 360 (6393): 1133-1136. ... "Blood Transfusion, Haemophilia, and AIDS". The Lancet. 324 (8417-8418): 1433-1435. December 1984. doi:10.1016/s0140-6736(84) ... demonstrated how the use of new technologies makes it possible based on a blood test among pregnant woman to predict ... infected with HIV and later the same year that HIV infection was caused by commercially manufactured factor VIII based on blood ...
"Fetal genotyping for the K (Kell) and Rh C, c, and E blood groups on cell-free fetal DNA in maternal plasma". Transfusion. 47 ( ... Fetal antigen status can be tested as early as 15 weeks by PCR of fetal cells. CVS is possible as well to test fetal antigen ... as well as fetal signs of anemia or hydrops. Blood is generally drawn from the father to help determine fetal antigen status. ... Blood is taken from the mother during the pregnancy, and using PCR, can detect the K, C, c, D, and E alleles of fetal DNA. This ...
Blood is taken from the mother, and using PCR, can detect the K, C, c, D, and E alleles of fetal DNA. This blood test is non- ... Fetal antigen status can be tested as early as 15 weeks by PCR of fetal cells. CVS is possible as well to test fetal antigen ... "Fetal genotyping for the K (Kell) and Rh C, c, and E blood groups on cell-free fetal DNA in maternal plasma". Transfusion. 47 ( ... For US patients, blood may be sent to either of the labs. In the US, Sensigene is done by Sequenome to determine fetal D status ...
Circulating cffDNA can be detected in maternal blood between the 5th and the 7th week of gestational age, however more fetal ... Wright, Caroline F.; Burton, Hilary (2009). "The use of cell-free fetal nucleic acids in maternal blood for non-invasive ... Clausen, Frederik Banch (2014). "Integration of noninvasive prenatal prediction of fetal blood group into clinical prenatal ... "Translating cell-free fetal DNA technology: structural lessons from non-invasive RhD blood typing". Trends in Biotechnology. 31 ...
Blood is taken from the mother, and using PCR, can detect the K, C, c, D, and E alleles of fetal DNA. This blood test is non- ... Fetal antigen status can be tested as early as 15 weeks by PCR of fetal cells. CVS is possible as well to test fetal antigen ... "Fetal genotyping for the K (Kell) and Rh C, c, and E blood groups on cell-free fetal DNA in maternal plasma". Transfusion. 47 ( ... For US patients, blood may be sent to either of the labs. In the US, Sensigene is done by Sequenome to determine fetal D status ...
Aickin, Donald Russell (1972). Prediction of fetal risk by maternal blood oestrogen measurement (MD). University of Melbourne. ... the title of his MD thesis was Prediction of fetal risk by maternal blood oestrogen measurement. In 1972, Aickin was appointed ...
This screening can also provide information about fetal sex and rhesus (Rh) blood type. A blood sample is drawn from the ... The amount of fetal DNA is assessed to determine if there is extra fetal genetic material present that may indicate an ... The testing is performed from a few drops of blood collected in the newborn period, often by a heel stick. The exact method of ... October 2016). "Noninvasive prenatal screening for fetal aneuploidy, 2016 update: a position statement of the American College ...
... is more abundant in fetal blood, hence the name "fetuin" (from Latin, fetus). Fetal bovine serum contains more fetuin ... Fetuins are blood proteins that are made in the liver and secreted into the bloodstream. They belong to a large group of ... The best known representative of carrier proteins is serum albumin,[citation needed] the most abundant protein in the blood ...
The blood vessel formations associated with SWS start in the fetal stage. Around the sixth week of development, a network of ... There is also malformation of blood vessels in the pia mater overlying the brain on the same side of the head as the birthmark ... This reduces the amount of oxygen and blood flowing to the brain, which can affect brain tissue development.[citation needed] ... The symptoms can include glaucoma, cerebral blood flow abnormalities and headaches. More research is needed on this type of ...
November 2005). "MEK kinase 1 activity is required for definitive erythropoiesis in the mouse fetal liver". Blood. 106 (10): ... doi:10.1182/blood-2005-04-1739. PMID 16081685. S2CID 19307521. Labuda T, Christensen JP, Rasmussen S, Bonnesen B, Karin M, ...
Thus, villi are part of the border between maternal and fetal blood during pregnancy. Villi can also be classified by their ... The bulk of the villi consist of connective tissues that contain blood vessels. Most of the cells in the connective tissue core ... Chorionic villi are villi that sprout from the chorion to provide maximal contact area with maternal blood. They are an ... Branches of the umbilical arteries carry embryonic blood to the villi. After circulating through the capillaries of the villi, ...
... but had traveled from the fetal blood into the maternal blood through the placenta. The paternal DNA in the mother's plasma had ... 6,258,540, which claims methods of using cell-free fetal DNA (cffDNA) circulating in maternal plasma (cell-free blood) to ... Then, they could reliably identify fetal DNA, which would in turn allow them to diagnose certain fetal genetic conditions such ... point of the invention is that the inventors discovered in 1996 that fetal DNA might be floating around in the mother's blood ( ...
Increased expression of the PTGS2 gene in the fetal membranes is connected to the presence of inflammation, causing uterine ... particularly prostacyclin which is found in blood vessel lining. Prostacyclin relaxes or unsticks platelets, so selective COX-2 ...
Doppler sonography can be used to evaluate the pulsations in the fetal heart and bloods vessels for signs of abnormalities. ... Fetal number, including number of amnionic sacs and chorionic sacs for multiple gestations Fetal cardiac activity Fetal ... "Fetal Keepsake Videos". Food and Drug Administration. Retrieved 2011-05-21. "Statement on Measurement of the Fetal Heart Rate ... Fetal number, including number of amnionic sacs and chorionic sacs for multiple gestations Embryonic/fetal cardiac activity ...
Susan E, Wert (2004). Fetal and Neonatal Physiology (3rd ed.). Bradley P. Fuhrman and Jerry J. Zimmerman. (2011). Pediatric ... 2‐dimensional color Doppler imaging could visually capture the blood flow, hence determine the existence of pulmonary ... Diagnosis and Management of the Fetal Patient". Acta Endocrinologica (Bucharest). 9 (4): 657. doi:10.4183/aeb.2013.657. ISSN ... therapy/fetal-interventions-procedures/ex_utero_intrapartum_t reatment.html Sadiqi, Jamshid; Hamidi, Hidayatullah (October 30, ...
... such as bowing the head and clasping the hands together in an almost fetal position, are suggestive of "social touch" actions. ... due to factors such as increased blood flow to the head and nasal breathing. Overall, slight health benefits have been found ...
He asserts that the Borborites engaged in a version of the eucharist in which they would smear their hands with menstrual blood ... The consumption of seminal and fetal material, as a microcosm of Barbelo's seduction of the archons to recover captive light, ... they would take her menstrual blood and everyone in the church would eat it as part of a sacred ritual. The Borborites were ... and semen and consume them as the blood and body of Christ respectively. He also alleges that, whenever one of the women in ...
Goldscheider, Eric (10 August 2003). "Fetal positions". Boston Globe. Retrieved 2 October 2009. "NOVA Online/Odyssey of Life/ ... enabled him to make groundbreaking photographs of living human blood vessels and body cavities. He achieved international fame ...
In addition, high expression of Granulysin can be found in the placenta to protect fetal epithelial cells. The 15 kDa GNLY was ... Patients with high levels of Granulysin in blood serum are better able to fight off metastasis, and generally progression of ...
"Fetal distress increases interleukin-6 and interleukin-8 and decreases tumour necrosis factor-alpha cord blood levels in ... Risk factors for fetal birth injury include fetal macrosomia (big baby), maternal obesity, the need for instrumental delivery, ... More invasive monitoring can involve a fetal scalp electrode to give an additional measure of fetal heart activity, and/or ... similar to a Doppler fetal monitor, that continuously emits ultrasound and detects motion of the fetal heart by the ...
In science class, Thayer drinks the blood of a dead fetal pig in an effort to impress his fellow students. Instead of being ...
... detect fetal abnormalities, and locate blood clots without an operation. In the 1980s, PNL researchers introduced the first ... "Portable Blood Irradiator." Radiation Protection Dosimetry 60(4): 359-362. PNNL: Pacific Northwest Center for Global Security ... portable blood irradiator for leukemia treatments, and worked with the Fred Hutchinson Cancer Research Center in Seattle under ...
Her "horses" tend to get in a fetal position or birthing position and cry tears of pain. Common syncretization is as the Virgin ... Her favourite sacrifices include black pigs, griot (seasoned fried pork), blood (seven stabs of the sword), and rum. Ti Jean ...
Paroxysmal nocturnal hemoglobinuria is characterized by red blood cell destruction, anemia (red blood cells unable to carry ... Pegcetacoplan may cause fetal harm so it should be avoided in pregnant patients. Pegcetacoplan may also interfere with silica ... "FDA approves new treatment for adults with serious rare blood disease". U.S. Food and Drug Administration (FDA). 14 May 2021. ... Patients with PNH have greater and uninhibited complement activity, which may leads to intravascular (inside blood vessels) or ...
Peripheral blood is collected for karyotyping. This helps classify the patient in one of the three main categories of DSD: ... In the normal prenatal stages of fetal development, the fetus is exposed to testosterone - albeit more in male fetuses than ... This is a more specific procedure in the detection of the defect in comparison to analysing blood. The treatment and/or ... Palpation of gonads from the labioscrotal fold to the abdomen (inguinal canal). Hydration and blood pressure assessment should ...
The levels of inflammatory cytokines (e.g., IL-1β, IL-2, IL-6, and TNF-α) in blood plasma are affected by zinc deficiency and ... Shah D, Sachdev HP (January 2006). "Zinc deficiency in pregnancy and fetal outcome". Nutrition Reviews. 64 (1): 15-30. doi: ... Diagnosis is typically made based on clinical suspicion and a low level of zinc in the blood. Any level below 70 mcg/dl (normal ...
Peripheral blood cells from the sampled patients confirmed decreased levels of the mutant RNA transcript, consistent with ... A significant level of SON expression in fetal tissue has suggested a regulatory role of SON in cellular proliferation and or ... failure of neurons to migrate properly during early development of the fetal brain. Ventriculomegaly can also be observed in ... ZTTK syndrome patients is Arnold-Chiari malformations which are structural defects in the cerebellum that manifest during fetal ...
Wangikar, P.B; Dwivedi, P; Sinha, N; Sharma, A.K; Telang, A.G (2005). "Effects of aflatoxin B1 on embryo fetal development in ... Sur, E; Celik, İ (2003). "Effects of aflatoxin B1on the development of the bursa of Fabricius and blood lymphocyte acid ... Teratogenicity The teratogenic effects of aflatoxin B1 in rabbits have been reported to include reduced fetal weights, wrist ...
Blood vessel dysfunction and clot formation (as suggested by high D-dimer levels caused by blood clots) may have a significant ... Some examples of these could be foetal growth restriction, preterm birth, and perinatal mortality, which refers to the foetal ... The virus may also enter the bloodstream from the lungs and cross the blood-brain barrier to gain access to the CNS, possibly ... A low level of blood lymphocytes may result from the virus acting through ACE2-related entry into lymphocytes. Another common ...
In short, there is still a chance for an ill liver to get rid of excessive unconjugated bilirubin in the blood plasma, ... Bhutani, Vinod K.; Wong, Ronald (2015). "Bilirubin-induced neurologic dysfunction (BIND)". Seminars in Fetal and Neonatal ... Because conjugated bilirubin is water soluble and so does in blood. Divers, Thomas J.; Barton, Michelle Henry (2018). " ... Cashore, William J. (2017). "Neonatal Bilirubin Metabolism". Fetal and Neonatal Physiology. Elsevier. pp. 929-933. doi:10.1016/ ...
1992). "Genomic organization of human fetal specific P-450IIIA7 (cytochrome P-450HFLa)-related gene(s) and interaction of ... This control becomes an important factor in several processes, such as blood pressure regulation, clotting, and inflammatory ...
... blood acidosis at birth in human fetuses. As saltatory patterns preceded late decelerations of fetal heart rate (FHR) in the ... The first commercial fetal monitor (Hewlett-Packard 8020A) was released in 1968. CTG monitoring is widely used to assess fetal ... Fetal stethoscope Nonstress test (NST) Biophysical profile (BPP) Ayres-de-Campos, Diogo (June 2018). "Electronic fetal ... A saltatory pattern of fetal heart rate is defined in cardiotocography (CTG) guidelines by FIGO as fetal heart rate (FHR) ...
She performed fetal stem cell transplants given before birth to treat inherited diseases of the immune system such as Wiskott- ... Blood and Marrow Transplantation and Cellular Therapy) at Stanford University. She is also the Director of the Stanford ...
Dhallan, Ravinder (2007). "A non-invasive test for prenatal diagnosis based on fetal DNA present in maternal blood: a ... "A non-invasive test for prenatal diagnosis based on fetal DNA present in maternal blood: a preliminary study" The Times " ... "A noninvasive test for prenatal diagnosis based on fetal DNA present in maternal blood: a preliminary study". The Lancet. 369 ( ... The company's core technology is based on its ability to increase the percentage of fetal DNA that is found in the maternal ...
The peptide endothelin constricts muscles in blood vessels, increasing blood pressure. Ambrisentan, which relaxes those muscles ... These physiologic effects are vital for the development of the fetal cardiopulmonary system. In addition to this, endothelin ... de Raaf MA, Beekhuijzen M, Guignabert C, Vonk Noordegraaf A, Bogaard HJ (2015). "Endothelin-1 receptor antagonists in fetal ...
Blood. 92 (9): 3057-3063. doi:10.1182/blood.V92.9.3057. PMID 9787139. Huehns, E.R.; Flynn, F.V.; Butler, E.A.; Beaven, G.H. " ... is a form of hemoglobin existing at low levels during embryonic and fetal life. It is composed of two alpha chains and two ... Blood. 97 (4): 1099-1105. doi:10.1182/blood.V97.4.1099. PMID 11159543. J. Eric Russell; Stephen A. Leibhaber (November 1998). " ... Embryonic hemoglobin is a tetramer produced in the blood islands in the embryonic yolk sac during the mesoblastic stage (from ...
Brodsky RA (November 2015). "Complement in hemolytic anemia". Blood. 126 (22): 2459-65. doi:10.1182/blood-2015-06-640995. PMID ... Levine F. "Basic Genetic Principles". Fetal and neonatal physiology (Fifth ed.). Philadelphia, PA. pp. 1-13. doi:10.1016/B978-0 ... When CD55 is absent, the complement system attacks red blood cells and causes them to be destroyed (hemolysis). CHAPLE syndrome ...
Premature rupture of fetal membranes (or PROM as it is more commonly referred) occurs in roughly 10% of pregnancies and is one ... PAMG-1 is present in blood and the amniotic fluid and cervico-vaginal discharge of pregnant women. The concentration of PAMG-1 ... PAMG-1 is an important biomarker for the detection of premature rupture of fetal membrane (PROM) The high concentration of PAMG ... report, "That the PartoSure test was found to be statistically superior to fFN fetal fibronectin and CL [cervical length ...
Khlat M (December 1989). "Inbreeding effects on fetal growth in Beirut, Lebanon". American Journal of Physical Anthropology. 80 ... so as to keep the Ptolemaic blood "pure" and to strengthen the line of succession. King Tutankhamun's mother is reported to ... Intellectual inbreeding Legality of incest List of coupled cousins Mahram Outbreeding depression Outcrossing Proximity of blood ...
... rarely high blood pressure. May increase or decrease liver function in some people. The side effects of low-dose doxepin for ... although the available evidence suggests it is unlikely to cause negative effects on fetal development. The lack of evidence ... Low blood pressure, (if patient arises too fast from the lying/sitting position to standing-known as orthostatic hypotension), ... introducing blood level monitoring and genotype testing". Journal of Dermatological Treatment. 33 (1): 1-7. doi:10.1080/ ...
... bleeding to mild vaginal bleeding Slightly tender uterus Normal maternal blood pressure and heart rate No coagulopathy No fetal ... decreased fetal movement decreased fetal heart rate. Vaginal bleeding, if it occurs, may be bright red or dark. A placental ... The accumulating blood pushes between the layers of the decidua, pushing the uterine wall and placenta apart. When the placenta ... Treatment may require blood transfusion or emergency hysterectomy. Placental abruption occurs in about 1 in 200 pregnancies. ...
A small puncture is made in the scalp and fetal blood droplets are collected ... Fetal blood testing is performed during labor to test the blood pH of the baby which can determine its well-being during ... Fetal blood testing is performed during labor to test the blood pH of the baby which can determine its well-being during ... A small puncture is made in the scalp and fetal blood droplets are collected in a thin glass tube. Testing the scalp pH can ...
Correlation between maternal and fetal umbilical cord blood lead concentrations in Libya  ... Placental tissue and cord blood from women with preterm and term labour were examined for microorganisms and compared in this ... Randomized placental and cord blood sampling culture in women with preterm and term labour to detect infection  ...
An intrauterine transfusion provides blood to an Rh-positive fetus when fetal red blood cells are being destroyed by the Rh- ... An intrauterine blood transfusion may be done to replace fetal red blood cells that are being destroyed by an Rh-sensitized ... An intrauterine transfusion provides blood to an Rh-positive fetus when fetal red blood cells are being destroyed by the Rh- ... An intrauterine fetal blood transfusion is done in the hospital. The mother may have to stay overnight after the procedure. ...
Associations between diet, lifestyle factors, blood glucose and fetal outcome in normal and diabetic pregnant women ...
Linear regression revealed that neonatal umbilical cord blood lead levels reflected the levels in maternal blood. Blood lead in ... of that in maternal blood, and lead concentration in neonatal blood from Ghadames was about 76.82% of that in maternal blood. ... Correlation between maternal and fetal umbilical cord blood lead concentrations in Libya ... Several studies have investigated lead levels in the blood of pregnant mothers and their fetal umbilical cord in different ...
Periosteum of fetal vertebral bodies are distributed originating from fine arteries of 70 - 120 diameter and arterioles of 30 ... Conclusions: Micro blood circulatory system of the vertebrae has angion structure. The peripheral zone of perichondrial vessels ... Results: Three concentric zones could be distinguished in the vascular architecture of the fetal vertebral bodies. 1) The ... The central zone of vertebral body is supplied with blood by the main arteriole. ...
Fetal Dopplers : Schiller Monitors : Medical Device Depot, Inc. ... Fetal Dopplers *Fetal Doppler Accessories & Probes *MedaSonics ...
... ... Title : Interdependence of blood pressure, weight gain, and fetal weight during normal human pregnancy. Personal Author(s) : ... Interdependence of blood pressure, weight gain, and fetal weight during normal human pregnancy. ... Blood Pressure Body Weight Female Humans Infant, Newborn Parity Pregnancy Research Article Time Factors ...
Prot#ART-0006: MELISSA Study: MatErnal Blood IS Source to Accurately diagnose fetal aneuploidy. A Prospective, Multi-Center ...
Fetal cells in maternal blood for prenatal diagnosis. Indian Journal of Human Genetics. 1999 Jan; 5(1): 1-14. ... This article thus reviews the latest literature on fetal cells from maternal blood with an intention of pursuing research with ... The presence of fetal cells in maternal circulation as early as 6 weeks of gestation has opened new avenues of noninvasive ... Fetal cells are one of the sources for prenatal diagnosis. Standard prenatal genetic diagnosis currently involves chorion ...
Correlation between maternal and fetal umbilical cord blood lead concentrations in Libya  Hafsa Alemam; Nabil Enattah; ... Aims: To estimate and correlate maternal and neonatal blood lead levels and compare these between neonates and their mothers in ... Tripoli and Ghadames.Methods: In this cross-sectional study, blood was collected from 120 and 116 mothers and their neonates ...
Percutaneous umbilical blood sampling (PUBS) or fetal scalp blood sampling is not recommended, as it is not helpful in ... specifically the use of fetal scalp electrodes, fetal blood sampling, ventouse delivery, and rotational forceps. ... Cordocentesis and fetal scalp blood sampling should be avoided in the management of the fetus/neonate of a mother with ITP in ... Antenatal management in fetal and neonatal alloimmune thrombocytopenia: a systematic review. Blood. 2017 Mar 16. 129 (11):1538- ...
blood-brain barrier; brain damage; early-onset fetal growth restriction; fetal hypoxia; late-onset fetal growth restriction; ... Retardo do Crescimento Fetal/sangue; Retardo do Crescimento Fetal/diagnóstico; Retardo do Crescimento Fetal/genética; Retardo ... Does the Blood-Brain Barrier Integrity Change in Regard to the Onset of Fetal Growth Restr ... Does the Blood-Brain Barrier Integrity Change in Regard to the Onset of Fetal Growth Restriction? ...
Only fetal NSE and MCA levels were positively correlated with those in maternal serum. Contrary to adult samples, no difference ... was demonstrated between male and female fetal levels of CA 125. MCA was the only maternal marker that increased significantly ...
New life-saving treatments for none in clinical trial on Collection of Maternal and NeoNatal Blood to Establish The Ontogeny of ...
The inner medullas relatively hypoxic, hypertonic, and acidotic environment is known to predispose to sickling of red blood ... Fetal hemoglobin in sickle cell anemia. Blood. 2011 Jul 7. 118 (1):19-27. [QxMD MEDLINE Link]. [Full Text]. ... While blood transfusions provide a higher proportion of HbA compared with patients own blood, ESAs likely do not provide a ... Blood pressures in the range defined as normal for the general population may thus represent hypertension in patients with SCD. ...
A fetal blood sample showed moderate anemia. Because of these negative results for virus infections, an ascitic fluid sample ... A fetal blood sample showed increased anemia and thrombocythemia.. Fetopathologic examination showed hepatosplenomegaly, thymic ... A) Fetal brain at 23... Table. * Table. Characteristics of neonates with congenital viral or bacterial infections, including a ... Fetal brain and placenta biopsy specimens and a serum sample from the mother were negative for LCMV. The matching sequence was ...
Following decreases in blood volume the fetal liver acts as a blood reservoir by releasing blood into the circulation. It ... A blood sample was taken to measure counts per ml of blood, the fetus killed and the remainder of the liver weighed. Blood ... Under normal conditions fetal blood volume was 5.1 ± 0.2 ml and the liver contained 22.7 ± 1.8% of total blood volume. This ... These results indicate the fetal liver is relatively larger than the adult and contains more blood per gram of tissue. ...
Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained ... "Fetal Blood" by people in this website by year, and whether "Fetal Blood" was a major or minor topic of these publications. ... "Fetal Blood" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Fetal Blood" by people in Profiles. ...
... cord blood; fetal transfer; manganese; toenails ... Cord blood was collected at the time of delivery. Maternal ... The main objective of this paper is to evaluate infant toenails, hair, and cord blood as biomarkers of prenatal exposure to As ...
Access to Kleihauer-Betke Test for Fetal Red Cells, Blood is restricted. Sign up now. ... Access to Kleihauer-Betke Test for Fetal Red Cells, Blood is restricted. Sign up now. ... Access to Kleihauer-Betke Test for Fetal Red Cells, Blood is restricted. Sign up now. ... Access to Kleihauer-Betke Test for Fetal Red Cells, Blood is restricted. Sign up now. ...
... refers to three techniques used to gain access to fetal blood ... Fetal blood transfusion. Fetal blood transfusion. A fetal blood ... Fetal anemia occurs when the babys blood count is too low. The condition can be life threatening, which is why fetal blood ... Our high definition Blood Bank is a hub of activity at all times, conducting vital blood tests and transfusion to save precious ... We have a dedicated team of excellent pathologists who assist gynaecologists in the blood management of fetuses.. ...
All of our products are handmade from A-Z. This means that we sculpt our own designs, make our own molds, hand pour and paint each cast.Slight differences are to be expected, as each piece is handmade and painted. These differences make for individualized designs.
Request for Fetal-Maternal-Hemorrhage/Cord Blood Testing. Attached Files. File. Action. ...
Advanced medical and surgical techniques for fetal care. ... Fetal Blood Transfusion. *Treatment for fetal thrombocytopenia ... Director, Fetal Therapy & Complex Obstetric Surgery Our Fetal Therapy program is one of a few in the nation offering the most ... Advancing the field of maternal fetal medicine is an important part of our mission. The Center for Advanced Fetal Care is part ... We have years of experience using invasive fetal management as well as minimally invasive techniques and sono-fetoscopy to ...
Cord blood parameters and fetal outcome in neonates with fetal distress due to meconium staining liquor- an observational study ... Cord blood parameters and fetal outcome in neonates with fetal distress due to meconium staining liquor- an observational study ... fetal cord blood parameter after birth (cord blood pH and lactate) and the neonatal outcome. ... The aim of this study was to analyse and compare the mode of delivery, cord blood parameters and fetal outcomes in cases ...
Results: Brain activity and blood flow were shown to be intimately linked. We observed a significant decrease in fetal brain ... Objectives: This study aimed to observe the effect of maternal cognitive activity on fetal brain blood flow to determine ... Conclusions: A correlation between the mothers engagement in mathematical activities and fetal brain blood flow may lead to ... Fetal middle cerebral artery (MCA), pulsatility index (PI) and peak systolic velocity (PSV) were monitored before, during, and ...
1996). Rare event selection of fetal nucleated erythrocytes in maternal blood by flow cytometry . 23(3), 218-227. 10.1002/(SICI ... 1996). Rare event selection of fetal nucleated erythrocytes in maternal blood by flow cytometry . 23(3), 218-227. 10.1002/(SICI ... Rare event selection of fetal nucleated erythrocytes in maternal blood by flow cytometry Article ... and LDS-751 staining and detection of fetal cells by gamma globin expression were also analyzed. Fetal cells were detected by ...
  • An intrauterine transfusion provides blood to an Rh-positive fetus when fetal red blood cells are being destroyed by the Rh-sensitized mother's immune system. (
  • Fetal blood sampling (FBS) shows that the fetus has severe anemia. (
  • Heavy metals accumulate in the maternal blood circulation system and reach the fetus through the placenta (12), leading to negative effects on pregnancy progression and fetal growth, such as spontaneous abortion, stillbirth, neonatal death, fetal physical dysmorphology, and mental retardation (13,14). (
  • Note - Conditions arising in the perinatal - - fetus, fetal period, even though death or morbidity - - - causing disproportion occurs later, should, as far as possible, be - - - - affecting fetus or newborn P03.1 coded to chapter XVI, which takes - - forces of labor precedence over chapters containing codes - - - affecting fetus or newborn P03.6 for diseases by their anatomical site. (
  • Blood of the fetus. (
  • A blood sample was taken to measure counts per ml of blood, the fetus killed and the remainder of the liver weighed. (
  • A fetal blood transfusion is a procedure used to treat anemia in the fetus. (
  • This single-center retrospective study included all women with a singleton fetus who had at least one fetal blood sample taken during labor. (
  • But researchers have identified factors in the blood that signal a fetus has Zika-related birth defects, helping mothers decide whether to terminate a pregnancy. (
  • We conclude that the placenta protects the developing fetus from maternal changes in serum BDNF that could otherwise have adverse consequences for fetal development. (
  • Pregnancy is a unique state where the blood systems of two individuals, the mother and the fetus, are intimately associated but not in direct contact. (
  • Then, if the slaughterhouse has the proper facilities, the entire reproductive tract including the fetus is cut quickly out of the carcass of the mother cow and brought to a more septic area (called: calf processing area) where the following procedure is followed to obtain its blood. (
  • The heart of the fetus must function in order to obtain an adequate (read: commercially satisfactorily) amount of fetal blood for FBS production. (
  • Alpha thalassemia major (four gene alpha thalassemia, or homozygous alpha thalassemia) interferes with the fetus' ability to make red blood cells. (
  • The exact mechanism through which the growth factors secreted by the natural killer cells promote fetal growth is not clear but it could be that they directly cross over from the mother to the fetus, or indirectly promote the growth of the placenta and blood vessels, thereby improving the nourishment to the fetus. (
  • Gilts were slaughtered at d 35 of pregnancy and a blood sample was collected from each fetus. (
  • This occurs because the lungs are not participating in gas exchange so the fetus relies on its mother fs placenta to enrich blood with oxygen. (
  • This just in: Dutch researchers have created a noninvasive blood test that has a near 100% accuracy in determining the gender of a fetus at only five weeks gestation. (
  • The simple blood test can also determine the presence of genetic abnormalities a fetus. (
  • Lead fetus through the placenta ( 12 ), leading to negative effects is a toxic heavy metal that is widely used in different on pregnancy progression and fetal growth, such as industries and it has no known function in the human spontaneous abortion, stillbirth, neonatal death, fetal body. (
  • A heart rate of less than 120 indicates fetal hypoxia, a dangerous pathological process characterized by a decreased supply of oxygen to the fetus. (
  • Often, oxygen therapy and hyperbaric oxygenation (pressure chamber) are also prescribed, which allows increasing blood oxygenation not only in the mother's body but also in the fetus. (
  • If no measures to normalize the oxygen supply to the fetus have given the desired effect or the symptoms of fetal hypoxia persist for more than twenty-eight weeks of pregnancy, it is best to have a cesarean section immediately. (
  • IPS has been noted to develop in a fetus following twin-to-twin transfusion, and authors have, in that case, postulated that the elevated blood volume/pressure may be a factor in the pathogenesis. (
  • [7] When the fetal stage commences, a fetus is typically about 30 mm (1.2 inches) in length, and the heart is beating. (
  • It has been proposed that lead is transferred across the placenta, probably by simple diffusion in the umbilical cord blood (9), and there is a suggestion that placental transfer of lead is facilitated by Ca2+ transport mechanisms during late gestation (10). (
  • Subsequently, the mother produces immunoglobulin G. The smaller size of this molecule permits passage across the placenta, resulting in the destruction of fetal platelets and neonatal thrombocytopenia. (
  • Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. (
  • Name two substances that leave the foetal blood through the placenta. (
  • One mechanism potentially accounting for the programming of disease risk is passage across the placenta of maternal factors impacting fetal development during sensitive periods, as has been suggested for the stress hormone cortisol 3 , 6 . (
  • Many studies have focused on maternal-to-fetal ZIKV transmission via blood and placenta. (
  • As part of normal fetal development, the PVR is very high to allow blood to preferentially flow to the placenta instead of to the lungs. (
  • El intercambio de nutrientes y desechos entre la sangre materna y fetal ocurre mediante la PLACENTA. (
  • Although the exact cause of preeclampsia is unknown, experts believe it may have something to do with a reduction in blood flow to the placenta, perhaps during early pregnancy. (
  • Preeclampsia causes the blood vessels of the placenta and uterus to constrict, which reduces blood flow to your baby. (
  • Ultimately, that can lead to poor fetal growth, not enough amniotic fluid, or placental abruption (separation of the placenta from the uterus). (
  • In this case, treatment is primarily aimed at normalizing the flow of blood to the uterus and placenta, but in case of acute fetal hypoxia, it is recommended to induce labor by artificial means, rather than using any treatment methods. (
  • Placental tissue and cord blood from women with preterm and term labour were examined for microorganisms and compared in this randomized prospective study. (
  • However, there was a significant correlation between maternal blood and umbilical cord blood in Tripoli and Ghadames. (
  • Linear regression revealed that neonatal umbilical cord blood lead levels reflected the levels in maternal blood. (
  • The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery. (
  • Phase I study of cord blood-derived natural killer cells combined with autologous stem cell transplantation in multiple myeloma. (
  • Yvon ES, Burga R, Powell A, Cruz CR, Fernandes R, Barese C, Nguyen T, Abdel-Baki MS, Bollard CM. Cord blood natural killer cells expressing a dominant negative TGF-ß receptor: Implications for adoptive immunotherapy for glioblastoma. (
  • PR1-specific cytotoxic T lymphocytes are relatively frequent in umbilical cord blood and can be effectively expanded to target myeloid leukemia. (
  • Enforced fucosylation of cord blood hematopoietic cells accelerates neutrophil and platelet engraftment after transplantation. (
  • Adoptive immunotherapy with the use of regulatory T cells and virus-specific T cells derived from cord blood. (
  • Low rate of infusional toxicity after expanded cord blood transplantation. (
  • The main objective of this paper is to evaluate infant toenails, hair, and cord blood as biomarkers of prenatal exposure to As and Mn and determine the relationship between maternal and infant As and Mn concentrations in these biomarkers. (
  • Cord blood was collected at the time of delivery. (
  • The aim of this study was to analyse and compare the mode of delivery, cord blood parameters and fetal outcomes in cases diagnosed with different degrees of meconium staining liquor. (
  • The cases were followed up and compared in thin or thick MSL group for the mode of delivery (normal vaginal delivery, instrumental delivery or caesarean section), fetal cord blood parameter after birth (cord blood pH and lactate) and the neonatal outcome. (
  • Maximum cases had normal cord blood parameters with a favourable neonatal outcome. (
  • Mazouri A, Fallah R, Saboute M, Taherifard P, Dehghan M. The prognostic value of the level of lactate in umbilical cord blood in predicting complications of neonates with meconium aspiration syndrome. (
  • Kumar N, Suman A, Sawant K. Relationship between immediate postpartum umbilical cord blood pH and fetal distress. (
  • We propose a relational and layered account of vulnerability and explore this concept using the case study of cord blood (CB) banking. (
  • We found no association between maternal symptoms of depression and either maternal or infant cord blood serum BDNF. (
  • We also compared the concentrations of insulin and IGFs in cord blood between the two groups. (
  • Over sixty hematology/blood count related tests on various specimens, including cord blood (post-delivery), fetal blood (in utero), and bone marrow. (
  • New laboratory panels, including the new 2014 HEDIS Value Sets, CBC with Differential panel for Cord blood , MICA (Major histocompatibility complex class I chain-related antigen A ) IgG antibodies, MERS (Middle East respiratory syndrome) coronavirus RNA panel, and a Mycobacterium tuberculosis stimulated gamma interferon & spot count panel. (
  • Safety of umbilical cord blood derived cells (UCBC) administration as determined by absence of any adverse events (AE) necessitating cessation of cell infusion (e.g. anaphylaxis or major change in cardio-respiratory status), or any AE attributable to cell administration within first 48 hours of infusion (e.g. bacterial infection). (
  • On the basis of almost 7000 cord blood samples from 20 cohorts worldwide, about 9000 methylation sites were found to be associated with gestational age at birth. (
  • The researchers found a clear overlap of methylation markers in cord blood, fetal brain tissue, and fetal lung tissue in relation to gestational age at birth, highlighting the consistency of these findings across tissues relevant for the health impacts of gestational age at birth. (
  • Interdependence of blood pressure, weight gain, and fetal weight during normal human pregnancy. (
  • The increased frequency in pregnancy could be due to frequent blood testing and unmasking of asymptomatic mild thrombocytopenia in pregnancy. (
  • Blood-brain barrier disintegration is not dependent on pregnancy advancement at the time of FGR diagnosis . (
  • Objectives: This study aimed to observe the effect of maternal cognitive activity on fetal brain blood flow to determine whether systematic maternal mathematical activity during pregnancy might influence child brain development. (
  • This is a rare blood disorder during pregnancy that can lead to brain haemorrhages in the baby. (
  • However, little is known about BDNF levels in the blood during pregnancy. (
  • Uterine natural killer cells secrete the growth factors pleiotrophin and osteoglycin that promote fetal growth and prevent retardation in the early months of pregnancy. (
  • During pregnancy, they promote the growth of blood vessels, improve birth weight and fetal growth. (
  • Retrieved on Dec 05, 2022 from (
  • Do I have to see a maternal-fetal medicine specialist for a twin pregnancy? (
  • Maternal-fetal medicine specialists see high-risk pregnancies, but not every twin pregnancy will fall into this category. (
  • Reginald Tucker] Cholera infections during pregnancy are associated with high rates of fetal death, especially when women are severely dehydrated. (
  • Although the relationship between fetal death and maternal age during cholera has not been documented, younger age is associated with increased risk for other adverse pregnancy outcomes. (
  • The relationship between fetal death and trimester of pregnancy is unclear. (
  • Science has shown that at the end of a mother's pregnancy, up to 6 percent of the DNA in her blood plasma comes from her baby. (
  • Preeclampsia, a condition that is characterized by high blood pressure during pregnancy, affects approximately 5 to 8 percent of all expectant mothers. (
  • Determining fetal hypoxia starting from the fifth month of pregnancy will not be difficult. (
  • More on pregnancy-specific distress: it is important to highlight certain features specific to the state of pregnancy and embryonic/fetal development that may have a bearing on studies of stress-related outcomes. (
  • For these reasons, we recommend that studies of stress in the context of pregnancy and fetal development should include a measure of pregnancy-specific stress (e.g. (
  • In terms of assessing aspects of maternal-placental-fetal stress biology during pregnancy, in addition to collecting stress-related biomarkers such as those related to glucocorticoid activity and pro-inflammatory state, it may be important to assess concentrations of placental corticotrophin-releasing hormone (CRH). (
  • Blood gas monitoring is used to assess acid-base status and to avoid hyperoxia and hypoxia as well as hypercapnia and hypocapnia. (
  • The hemolysis of red blood cells due to isoimmunisation results in fetal anaemia and hypoxia leading to fetal heart failure. (
  • Fetal death may occur due to fetal hypoxia and acidosis resulting from excessive maternal dehydration. (
  • A high nucleated red blood cell (NRBC) count at birth is taken as a biomarker of fetal hypoxia. (
  • Acute and chronic fetal hypoxia can lead to fetal death in the womb or death of the baby during the first week of life. (
  • How to determine fetal hypoxia? (
  • Animal studies have shown that exposure of fetuses to cortisol results in irreversible changes in fetal brain structure and function. (
  • The aim of the current study was to investigate changes in fetal NT-proBNP serum concentrations before and after intrauterine transfusions and to assess its value as a marker of fetal heart failure. (
  • The following timeline describes some of the specific changes in fetal anatomy and physiology by fertilization age (i.e. the time elapsed since fertilization). (
  • NME1 may serve as a biomarker useful in the prediction of fetal circulatory centralization and extremely low birth weight in pregnancies complicated by the early-onset FGR. (
  • Fetal cells were detected by FISH in 11 of 19 (CD71 selection) and in 13 of 15 (gamma selection) tandem pregnancies, These data support the possibility of a noninvasive method for isolation and analysis of fetal cells for prenatal diagnosis. (
  • Fetal death occurred in 141 of the 900 analyzed pregnancies, more often in women less than 20 years of age, in their third trimester, seeking treatment more than 24 hours after symptom onset, with severe dehydration or who vomited. (
  • Fetal death occurred in 141 of 900 pregnancies. (
  • Medical statistics show that in 10-15% of all pregnancies, fetal heart rate deceleration is observed. (
  • Ultrasound shows evidence of fetal hydrops , such as swollen tissues and organs. (
  • Inclusion criteria: Infants with brain injury associated with fetal stroke after confirmation of brain injury on postnatal imaging (Cranial ultrasound, MRI brain) soon after birth. (
  • Ultrasound evaluation provides an assessment of your baby's anatomy, along with follow-up ultrasounds to measure fetal well-being. (
  • The aim of study was to verify the reliability of quantification by immunoprecipitation of methylated fetal DNA in maternal blood in the prenatal diagnosis of 21 trisomy. (
  • Fetal Bovine Serum: Are Cell Cultures Cruelty Free? (
  • In this paper the focus is on the production method of fetal bovine serum (FBS) and its welfare and ethical implications. (
  • This is done by adding so called culture medium to the cells, usually combined with fetal bovine serum (serum is blood without cells and clotting factors). (
  • Fetal bovine serum contains many substances which are needed by cultured cells to grow and live properly. (
  • It is being used extensively both in research and (the life science-) industry, making fetal bovine serum a commercially interesting product. (
  • Fetal bovine serum is obtained in those areas of the world where bovines are kept in an extensive manner (e.g. continent of America, Australia, Southern Africa, CIS). (
  • Your work Afterlife "looks at the ethical ambiguities and challenges that accompany the use and manipulation of organisms, in particular the use of Fetal Bovine Serum (FBS) in cell and tissue culture. (
  • Fetal anemia occurs when the baby's blood count is too low. (
  • Virtua's maternal-fetal medicine doctors provide access to leading-edge prenatal tests and procedures to evaluate your baby's health and development. (
  • Scientists have discovered, she said, that a baby's fetal cells show up more often in a mother's healthy breast tissue and less often in a woman who has breast cancer (43 versus 14 percent). (
  • One kind of fetal cells that enter into the mother's body is the baby's stem cells. (
  • The baby's fetal stem cells can actually become the mother's own cells that make up her liver, heart, or brain. (
  • In what any ethicist might declare to be legitimate 'embryonic stem cell therapy,' the baby's fetal stem cells migrate to the mother's injured sites and offer themselves as a healing remedy, becoming part of the mother's very body. (
  • Pinctott calls the evidence "striking" that a baby's fetal cells "repair and rejuvenate moms. (
  • Both Johnson and Artlett defend the hypothesis that the baby's fetal cells have a beneficent purpose, not to hurt the mother, but to protect, defend, and repair her for the rest of her life, especially when she becomes seriously ill. (
  • This is typically seen as a reflection of birth events (delivery after 40 weeks gestation, meconium aspiration syndrome, infection such as Group B Streptococcus) or with congenital heart lesions, which the extra blood flow through the pulmonary circulation can stress the pulmonary blood vessels (premature closure of blood vessels, pulmonary venous abnormalities). (
  • To estimate and correlate maternal and neonatal blood lead levels and compare these between neonates and their mothers in Tripoli and Ghadames. (
  • Syed W, Liaqat N, Qazi Q, Yasmeen S. Relationship between immediate postpartum umbilical cord pH, fetal distress and neonatal outcome. (
  • Led by the Leiden University Medical Center (LUMC), the first international multicenter study into treatment strategies and outcomes for babies with fetal and neonatal allo-immune thrombocytopenia (FNAIT) has been published in The Lancet Haematology. (
  • In addition, blood lead levels in neonatal umbilical cord and mothers in the concentration was inversely and significantly associated coastal city of Tripoli and desert city of Ghadames. (
  • In April 2012, MSF established a CTC to improve fetal outcomes in pregnant women by facilitating intensive follow- up for dehydration and rapid access to obstetric and neonatal services. (
  • We have a dedicated team of excellent pathologists who assist gynaecologists in the blood management of fetuses. (
  • Blood samples were measured for hematocrit, cell number, hemoglobin, % nucleated cells, % reticulocytes and erythropoietin (largest and smallest fetuses only). (
  • The fetal serum concentration of NT-proBNP appears to be a satisfactory marker for heart failure in fetuses inflicted with severe anaemia caused by hemolytic disease. (
  • He helped to describe the exchange of blood between pregnant women and their fetuses. (
  • By 2002, Lo distinguished the DNA differences between pregnant women and their fetuses, enabling scientists to identify fetal DNA in pregnant women's blood. (
  • Additionally, in late-onset FGR, the higher release of the S100B into the maternal serum in regard to fetal distress was observed. (
  • Fluctuations in maternal serum BDNF may consequently adversely influence fetal development. (
  • Once the blood has been obtained, it is allowed to clot at low temperature, after which process the clotted substance is seperated from the serum by refrigerated centrifugation. (
  • The serum is derived from the blood of fetal cows. (
  • While the idea of draining unborn calves of their blood may sound horrifying, the calves are essentially a bi-product of meat production and while their blood is harvested to produce serum, their bodies are discarded, deemed unfit for consumption. (
  • Serum creatinine levels, creatinine clearance, and blood urea nitrogen (BUN) levels suffice in most cases. (
  • To reduce the complications, in the last years different methods to detect fetal cells or DNA in maternal blood were developed. (
  • It also helps control blood pressure in women with preeclampsia by alleviating labor pain, and it blunts the hemodynamic effects of uterine contractions and the associated pain response in patients with other medical complications. (
  • Fetal complications can arise due to tangling of the umbilical cords or an imbalance in nutrients, blood or other vital life supporting systems. (
  • Transfusions can be given through the fetal abdomen or, more often, by delivering the blood into the umbilical vein or artery. (
  • The condition can be life threatening, which is why fetal blood transfusions need to be conducted shortly after discovering fetal anemia. (
  • Also, cordocentesis can be used to deliver blood transfusions and medication to the baby through the umbilical cord. (
  • Another fraction of the primitive blood cells remains hemoglobin-free. (
  • as, in addition to the production of hemoglobin-containing cells, they also give rise to megakaryocytes and diverse other elements in the yolk sack, which have nothing to do with the red blood corpuscles. (
  • Intrauterine crowding may accelerate blood development and interfere with hemoglobin synthesis. (
  • Hemoglobin concentration, red blood cells count, hematocrit and NT-proBNP concentrations were assessed in samples taken before and after each transfusion. (
  • That blood is called fetal hemoglobin. (
  • CTX001 uses the CRISPR gene-editing technology to increase the production of fetal hemoglobin in patients' red blood cells. (
  • Fetal hemoglobin (HbF) induction in erythroid progeny after base editing or nuclease editing was similar. (
  • The medium cerebral artery peak systolic velocity was 1.98 multiples of median, which indicated fetal anemia. (
  • We detected moderate blood lead levels among pregnant women in Tripoli and Ghadames. (
  • The patient was a 29-year-old G1P0 pregnant woman hospitalized at 23 weeks' gestation after routine ultrasonography because of fetal ascites and minor ventriculomegaly. (
  • For example, the common practice of slaughtering pregnant cows , and subsequent availability of fetal calf blood, has enabled great advancements in cell and tissue culture and contributed to the development of new medical technologies and treatments for humans and other organisms. (
  • A reduced number of the natural killer cells in pregnant mice resulted in severe fetal growth restriction and defective development of the fetal skeletal system. (
  • When such cells are transferred into pregnant mice with impaired fetal growth, the impairment of fetal growth could be reversed. (
  • The new procedure involves taking a blood plasma sample from the mom-to-be and then extracting the fetal DNA which circulates in the blood of a pregnant woman. (
  • In 1997, Lo discovered fetal DNA in maternal plasma, which is the liquid component of a pregnant woman's blood. (
  • Science has been studying the phenomena of fetal cell microchimerism for more than 30 years, after researchers at Stanford University were shocked in 1979 to discover a pregnant mother's blood containing cells with Y sex chromosomes. (
  • In their research, Johnson found that a teaspoon of blood from a pregnant mother contained "dozens, perhaps even hundreds of cells… from the baby. (
  • At the first manifestations of symptoms of fetal heart rate deceleration, a pregnant woman is immediately hospitalized. (
  • The peripheral zone of perichondrial vessels has combined blood supply surrounded by blindly ending capil lary network drained up and down from the central zone. (
  • O. Demed, Y. Damdinjav, A. Amgalanbaatar, E. Sundui and A. Dorjkhuu, "The Structure of Internal Blood Vessels in Mongolian Fetal Vertebral Bodies," Open Journal of Applied Sciences , Vol. 3 No. 1, 2013, pp. 62-66. (
  • In humans, there is a barrier between the maternal and fetal circulations composed of a single continuous layer of syncytiotrophoblast over a layer of villous cytotrophoblast covering the endothelial cell-lined fetal blood vessels 20 . (
  • With the first few breaths at birth, the lungs open up, the blood vessels dilate, the PVR begins to drop dramatically and blood flow is now directed to the lungs as the sole source of oxygen. (
  • As a result, there are fewer blood vessels in the lungs, and the PVR is naturally higher to start. (
  • There is some ability of the blood vessels to widen after birth, but it fs limited. (
  • The lungs and blood vessels are normally developed in these infants, but the blood vessels themselves are constricted or narrowed. (
  • The echocardiogram is the test used to make the diagnosis of PPHN, and it will show signs of elevated right ventricular pressure and abnormal shunting of blood through persistent fetal circulation vessels. (
  • In twin-to-twin transfusion syndrome (TTTS), the communication of placental vessels between the donor and recipient twin creates an imbalance of blood flow resulting in anemia in the donor and polycythemia in the recipient. (
  • The communication of placental vessels between the donor and recipient twin creates an imbalance of blood flow resulting in anemia in the donor and polycythemia in the recipient. (
  • The simple version of Vasa Previa is that fetal blood vessels are now embedded across my cervix. (
  • If my cervix were to dilate, those vessels would rupture and the baby would bleed out in minutes because he's so little and frankly, doesn't have much blood. (
  • Experimental construction of the pulmonary trunk in fetal lambs is accompanied by relatively thin-walled PA resistance vessels. (
  • Desai D, Maitra N, Patel P. Fetal heart rate patterns in patients with thick meconium staining of amniotic fluid and its association with perinatal outcome. (
  • A new NCHS report presents 2013 fetal and perinatal mortality data by maternal age, marital status, race, Hispanic origin, and state of residence, as well as by fetal birthweight, gestational age, plurality, and sex. (
  • Trends in fetal and perinatal mortality are also examined. (
  • An intrauterine fetal blood transfusion is done in the hospital. (
  • An intrauterine blood transfusion may be done to replace fetal red blood cells that are being destroyed by an Rh-sensitized mother's immune system (Rh disease). (
  • Fetal survival after transfusion depends upon the severity of the fetus's illness, the method of transfusion, and the skill of the doctor who does the procedure. (
  • Our high definition Blood Bank is a hub of activity at all times, conducting vital blood tests and transfusion to save precious little lives in the womb. (
  • Think of a randomized study in which two different blood transfusion strategies are compared. (
  • A small puncture is made in the scalp and fetal blood droplets are collected in a thin glass tube. (
  • Fetal scalp blood sampling: Do pH and lactates provide the same information? (
  • Gandhi G, Chandnani K. Association between clinical diagnosis of fetal distress with umbilical artery acidaemia at birth in women undergoing caesarean section for fetal distress. (
  • Periosteum of fetal vertebral bodies are distributed originating from fine arteries of 70 - 120 diameter and arterioles of 30 - 50 diameter which are part of arteries of network of micro blood circulation. (
  • W. R. Ferguson, "Some Observations on the Circulation in Fetal and Infant Spines," Journal of Bone and Joint Surgery, Vol. 32A, No. 3, 1950, pp. 640-648. (
  • The presence of fetal cells in maternal circulation as early as 6 weeks of gestation has opened new avenues of noninvasive approach to prenatal diagnosis in identifying successfully both Chromosome and molecular genetic abnormalities. (
  • Following decreases in blood volume the fetal liver acts as a blood reservoir by releasing blood into the circulation. (
  • A noninvasive method of prenatal genetic diagnosis requires fetal cell selection from the maternal circulation that allows efficient recovery for analysis by fluorescence in situ hybridization (FISH). (
  • We have solved several problems that negatively affect the isolation and FISH analysis of fetal nucleated red blood cells (nRBCs) in the maternal circulation. (
  • These metals accumulate in the in the maternal blood circulation system and reach the human body and cause serious health disorders ( 1 , 2 ). (
  • [6] , [7] Any distress during fetal development stimulates hepatic erythropoiesis which results in a high number of NRBC in the circulation. (
  • Some researchers postulate this decreased medial muscular layer is caused by a higher oxygen tension of the blood perfusing the fetal pulmonary circulation. (
  • and LDS-751 staining and detection of fetal cells by gamma globin expression were also analyzed. (
  • IMSEAR at SEARO: Fetal cells in maternal blood for prenatal diagnosis. (
  • The production of growth factors was less from the fetal tissue that has undergone miscarriage. (
  • Fetal growth can be terminated by various factors, including miscarriage , feticide committed by a third party, or induced abortion . (
  • The risk of miscarriage decreases sharply at the beginning of the fetal stage. (
  • Prot#ART-0006: MELISSA Study: MatErnal Blood IS Source to Accurately diagnose fetal aneuploidy. (
  • Meconium staining liquor (MSL) results from passage of meconium during labour and is used to diagnose fetal distress. (
  • These results indicate the fetal liver is relatively larger than the adult and contains more blood per gram of tissue. (
  • These products include fetal tissue, which is usually pinkish gray, as well as blood or blood clots. (
  • At this stage, pieces of fetal tissue that are too large to leave the cervix and body can become trapped inside the uterus, preventing a complete abortion. (
  • Infection after incomplete abortion is often the result of pieces of fetal tissue trapped in the uterus, and will often be accompanied by a high fever and heavy bleeding. (
  • Conclusions: A correlation between the mother's engagement in mathematical activities and fetal brain blood flow may lead to enhancement of the fetus's brain function and a cognitive advantage for the child. (
  • Now, the mother's plasma can be tested by way of a quick and easy blood test, only five weeks after conception occurs-a very short time indeed. (
  • The reason symptoms don't show up at birth is that people with sickle cell disease are protected from it if they're producing the kind of blood found in the mother's womb. (
  • Pinctott pointed out that as the quantity of fetal cells in a mother's body increase the activity of autoimmune conditions such as rheumatoid arthritis and multiple sclerosis decreases. (
  • One would expect them [the fetal cells in the mother's body] to be attacked fairly rapidly. (
  • Fetal infection. (
  • Our study was a positive vaginal culture who were not hospitalized, and two undertaken to delineate trends and better characterize the epi- with positive blood cultures whose hospital charts could not be demiologic and clinical features of this emerging infection in retrieved). (
  • Thus, it is possible that the initiation, if not the maintenance, of the fetal response to some maternal manipulations may be mediated by fetal perceptual detection of changes in the intrauterine milieu. (
  • Intrauterine therapy decreases the severity of anaemia and reduces the fetal heart failure index. (
  • These findings thus suggest that insufficient secretion of growth-promoting factors by a specific subset of natural killer cells may be responsible for restricted fetal development in humans. (
  • However, this elephant is the oldest of the four animals studied, and blood pressure parameters generally increase with age in humans and this may be the case with this elephant. (
  • In humans, during embryonic and fetal development, the liver is a major hematopoietic organ. (
  • In humans, the fetal stage of prenatal development begins about eight weeks after fertilization , when the major structures and organ systems have formed, until birth. (
  • Cordocentesis is also known as percutaneous umbilical blood sampling (PUBS), umbilical vein sampling, and fetal blood sampling. (
  • This article thus reviews the latest literature on fetal cells from maternal blood with an intention of pursuing research with this novel noninvasive approach, which is the need of today in India. (
  • We sought to evaluate a multiplexed massively parallel shotgun sequencing assay for noninvasive trisomy 21 detection using circulating cell-free fetal DNA. (
  • A number of clinical and laboratory studies are continuing throughout the world to determine the feasibility of isolation of fetal cells from maternal blood and its subsequent use in genetic diagnosis by FISH and PCR technology. (
  • Recovering and analysing fetal erythrocytes from maternal blood is being pursued for non‐invasive prenatal genetic diagnosis. (
  • Cordocentesis is a highly specialized prenatal test in which a fetal blood sample is removed from the umbilical cord and tested for genetic problems, infections, or anemia (low blood cell count). (
  • Genetic testing in patients with possible foetal alcohol spectrum disorder. (
  • With intravenous Disopyramide Phosphate, either increases in systolic/diastolic or decreases in systolic blood pressure have been reported, depending on the infusion rate and the patient population. (
  • Several attempts have been made to detect and retrieve fetal nucleated cells including nucleated erythrocytes (NRBCs), leucocytes and trophoblasts in maternal blood. (
  • Bilirubin production is elevated because of increased breakdown of fetal erythrocytes. (
  • This is the result of the shortened lifespan of fetal erythrocytes and the higher erythrocyte mass in neonates. (
  • A fetal blood sample showed moderate anemia. (
  • Hoffmann AL, Hjortdal JØ, Secher NJ, Weile B. The relationship between Apgar score, umbilical artery pH and operative delivery for fetal distress in 2778 infants born at term. (
  • The available data best support the hypothesis that the fetal effects were likely associated with modulation of the thyroid hormones by iodide during development. (
  • Although much of the earlier work in this area was limited by many conceptual and methodological problems, over the last two decades, larger, better-designed prospective, population-based human studies, as well as mechanistic ones focused on brain development and epigenetics, have provided further evidence in support of what is known as the prenatal programming hypothesis or fetal origins of adult disease hypothesis. (
  • An increase in the concentrations of blood parameters was strongly correlated with a decrease in the concentration of NT-proBNP. (
  • NT-proBNP concentrations were the grea test with the smallest Hb (4.0-5.9 g/dl), hematocrit and red blood cell (RBC) concentrations, respectively. (
  • [3] studied 98 twins and found a significant intertwin difference in the Log 10 (NRBC/100 white blood cell [WBC]), concluding that the smaller twins experienced a relative lack of oxygen compared with the larger twin in utero . (
  • The few literature citations involving blood pressure (BP) measurements have utilized direct arterial measurement of immobilized or stationary conditioned elephants. (
  • Furthermore, the purpose of the study was to investigate the relationship between the blood-brain barrier breakdown and neurological disorders in FGR newborns . (
  • The Dana-Farber/Boston Children's Cancer and Blood Disorders Center is an integrated pediatric hematology and oncology program through Dana-Farber Cancer Institute and Boston Children's Hospital. (
  • The Thalassemia Program at the Dana-Farber/Boston Children's Cancer and Blood Disorders Center treats all forms of thalassemia. (
  • Base editing by nucleotide deaminases linked to programmable DNA-binding proteins represents a promising approach to permanently remedy blood disorders, although its application in engrafting hematopoietic stem cells (HSCs) remains unexplored. (
  • As labor progresses, the descent of fetal head and subsequent pressure on the pelvic floor, vagina, and perineum, generates somatic pain, which is transmitted by the pudendal nerve (S2-4). (
  • Previously, the United States Centers for Disease Control and Prevention proposed a population-based paediatric blood lead concentration of 10 μg/dl as an acceptable level (6). (
  • NAIT is caused by maternal immunization against fetal paternally derived platelet-specific antigens (similar to rhesus [Rh] disease). (
  • Hydrops fetalis is sometimes used as a synonym for homozygous alpha thalassemia, a lethal or life-threatening disease of mid- and late fetal development caused by the inability to make red blood cells. (
  • Excipients Included in U.S. Vaccines, by Vaccine" on the official website of the United States Centers for Disease Control and Prevention lists as ingredients cell lines cultured from aborted fetal cells. (
  • N-terminal pro-B-type natriuretic peptide: a potential marker of fetal heart failure in hemolytic disease. (
  • Luterek K, Szymusik I, Bartkowiak R, Koltowski L, Filipiak K, Wielgos M. N-terminal pro-B-type natriuretic peptide: a potential marker of fetal heart failure in hemolytic disease. (
  • From the "Illustrated Manual for the Recognition and Diagnosis of Certain Animal Diseases", published in 1982, by the Mexico-United States Commission for the Prevention of Foot and Mouth Disease, taken during a fetal necropsy, this photograph depicted an aborted fetal ruminant with numerous hemorrhages, and an apparent hemothorax (blood within the thoracic cavity). (
  • FNAIT arises because the platelets, small cell fragments in our blood that prevent bleeding, of the unborn baby are broken down by antibodies from the mother. (
  • We report the observation of 46, XY/47, XXY mosaicism in fetal cells from a woman whose first‐trimester chorionic villus sampling (CVS) initially showed only 46, XY. (
  • This free bilirubin is able to cross lipid-containing membranes, including the blood-brain barrier, leading to neurotoxicity. (
  • Recent studies have indicated that exposures to methyl iodide (MeI) produce a number of effects in laboratory animals, including fetal toxicity, neurotoxicity, and degeneration of the nasal epithelium. (
  • Recent studies have indicated that exposures to methyl based pharmacokinetic (PBPK) modeling, and evaluat- iodide (MeI) produce a number of effects in laboratory ani- ing the relevance of the observations in animals to human mals, including fetal toxicity, neurotoxicity, and degenera- health. (
  • These studies have shown that there is no safe lead level for fetal intellectual development. (
  • The Center for Advanced Fetal Care is part of the North American Fetal Therapy Network (NAFTNet) and an active participant in the research and development of novel managements for complicated fetal conditions - meaning we continue to perfect new approaches to offer the earliest, most precise fetal testing and treatment. (
  • The development of the human blood-CSF-brain barrier. (
  • Pleiotrophin may be involved in blood vessel proliferation and the development of the brain, bone and cartilage, while osteoglycin takes part in the development of the heart, skin and eyes. (
  • Natural Killer Cells Promote Fetal Development through the Secretion of Growth-Promoting Factors. (
  • These data suggest that blood development in ME gilts is accelerated. (
  • Improvements in blood cell development could be beneficial to fetal survival in swine. (
  • The hypoxic, acidotic, and hyperosmolar environment of the inner medulla are known to promote sickling of red blood cells (RBCs) with resultant impairment in renal medullary blood flow, ischemia, microinfarction, and papillary necrosis. (
  • In addition, routinely monitor blood pressure, as infants with bronchopulmonary dysplasia can also develop systemic hypertension. (