Intracellular proteins that reversibly bind hydrophobic ligands including: saturated and unsaturated FATTY ACIDS; EICOSANOIDS; and RETINOIDS. They are considered a highly conserved and ubiquitously expressed family of proteins that may play a role in the metabolism of LIPIDS.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
A positively charged protein found in peripheral nervous system MYELIN. Sensitive immunological techniques have demonstrated that P2 is expressed in small amounts of central nervous system myelin sheaths of some species. It is an antigen for experimental allergic neuritis (NEURITIS, EXPERIMENTAL ALLERGIC), the peripheral nervous system counterpart of experimental allergic encephalomyelitis. (From Siegel et al., Basic Neurochemistry, 5th ed, p133)
Transport proteins that carry specific substances in the blood or across cell membranes.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
An unsaturated fatty acid that is the most widely distributed and abundant fatty acid in nature. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. (Stedman, 26th ed)
A group of fatty acids that contain 18 carbon atoms and a double bond at the omega 9 carbon.
FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.
FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.
A common saturated fatty acid found in fats and waxes including olive oil, palm oil, and body lipids.
A broad category of membrane transport proteins that specifically transport FREE FATTY ACIDS across cellular membranes. They play an important role in LIPID METABOLISM in CELLS that utilize free fatty acids as an energy source.
The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
An aspartate aminotransferase found in MITOCHONDRIA.
Cells in the body that store FATS, usually in the form of TRIGLYCERIDES. WHITE ADIPOCYTES are the predominant type and found mostly in the abdominal cavity and subcutaneous tissue. BROWN ADIPOCYTES are thermogenic cells that can be found in newborns of some species and hibernating mammals.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A group of fatty acids, often of marine origin, which have the first unsaturated bond in the third position from the omega carbon. These fatty acids are believed to reduce serum triglycerides, prevent insulin resistance, improve lipid profile, prolong bleeding times, reduce platelet counts, and decrease platelet adhesiveness.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
A clinical manifestation consisting of alterations in an individual's weight from his or her norm.
An antilipemic agent that lowers CHOLESTEROL and TRIGLYCERIDES. It decreases LOW DENSITY LIPOPROTEINS and increases HIGH DENSITY LIPOPROTEINS.
Salts and esters of the 16-carbon saturated monocarboxylic acid--palmitic acid.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Enzymes that catalyze the synthesis of FATTY ACIDS from acetyl-CoA and malonyl-CoA derivatives.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A group of 16-carbon fatty acids that contain no double bonds.
An 86-amino acid polypeptide, found in central and peripheral tissues, that displaces diazepam from the benzodiazepine recognition site on the gamma-aminobutyric acid receptor (RECEPTORS, GABA). It also binds medium- and long-chain acyl-CoA esters and serves as an acyl-CoA transporter. This peptide regulates lipid metabolism.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Family of MITES, in the superfamily Acaroidea, order Astigmata. They are frequently found in cereal-based foodstuffs including GRAIN and FLOUR.
A plant genus of the family CAMPANULACEAE that contains platycodin and other triterpenoid SAPONINS. It is a constituent of kikyo-to (MEDICINE, KAMPO).
Fats present in food, especially in animal products such as meat, meat products, butter, ghee. They are present in lower amounts in nuts, seeds, and avocados.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
Lipid infiltration of the hepatic parenchymal cells resulting in a yellow-colored liver. The abnormal lipid accumulation is usually in the form of TRIGLYCERIDES, either as a single large droplet or multiple small droplets. Fatty liver is caused by an imbalance in the metabolism of FATTY ACIDS.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
A family of enzymes that catalyze the stereoselective, regioselective, or chemoselective syn-dehydrogenation reactions. They function by a mechanism that is linked directly to reduction of molecular OXYGEN.
Long chain organic acid molecules that must be obtained from the diet. Examples are LINOLEIC ACIDS and LINOLENIC ACIDS.
The protein components of a number of complexes, such as enzymes (APOENZYMES), ferritin (APOFERRITINS), or lipoproteins (APOLIPOPROTEINS).
Leukocyte differentiation antigens and major platelet membrane glycoproteins present on MONOCYTES; ENDOTHELIAL CELLS; PLATELETS; and mammary EPITHELIAL CELLS. They play major roles in CELL ADHESION; SIGNAL TRANSDUCTION; and regulation of angiogenesis. CD36 is a receptor for THROMBOSPONDINS and can act as a scavenger receptor that recognizes and transports oxidized LIPOPROTEINS and FATTY ACIDS.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Proteins prepared by recombinant DNA technology.
S-Acyl coenzyme A. Fatty acid coenzyme A derivatives that are involved in the biosynthesis and oxidation of fatty acids as well as in ceramide formation.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
The rate dynamics in chemical or physical systems.
FATTY ACIDS which have the first unsaturated bond in the sixth position from the omega carbon. A typical American diet tends to contain substantially more omega-6 than OMEGA-3 FATTY ACIDS.
BUTYRIC ACID substituted in the beta or 3 position. It is one of the ketone bodies produced in the liver.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Measurement of the intensity and quality of fluorescence.
A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR ALPHA is important in regulation of GLUCOSE metabolism and CELL GROWTH PROCESSES. It is a target of THIAZOLIDINEDIONES for control of DIABETES MELLITUS.
A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.
A nuclear transcription factor. Heterodimerization with RETINOID X RECEPTOR GAMMA is important to metabolism of LIPIDS. It is the target of FIBRATES to control HYPERLIPIDEMIAS.
The differentiation of pre-adipocytes into mature ADIPOCYTES.
Proteins in the nucleus or cytoplasm that specifically bind RETINOIC ACID or RETINOL and trigger changes in the behavior of cells. Retinoic acid receptors, like steroid receptors, are ligand-activated transcription regulators. Several types have been recognized.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
An enzyme that catalyzes the first and rate-determining steps of peroxisomal beta-oxidation of fatty acids. It acts on COENZYME A derivatives of fatty acids with chain lengths from 8 to 18, using FLAVIN-ADENINE DINUCLEOTIDE as a cofactor.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Fatty acids which are unsaturated in only one position.
A group of compounds that are derivatives of octadecanoic acid which is one of the most abundant fatty acids found in animal lipids. (Stedman, 25th ed)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
An enzyme that catalyzes reversibly the conversion of palmitoyl-CoA to palmitoylcarnitine in the inner mitochondrial membrane. EC 2.3.1.21.
Diminished effectiveness of INSULIN in lowering blood sugar levels: requiring the use of 200 units or more of insulin per day to prevent HYPERGLYCEMIA or KETOSIS.
An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
An isoenzyme of creatine kinase found in the CARDIAC MUSCLE.
Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Established cell cultures that have the potential to propagate indefinitely.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
The sum of the weight of all the atoms in a molecule.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Short-chain fatty acids of up to six carbon atoms in length. They are the major end products of microbial fermentation in the ruminant digestive tract and have also been implicated in the causation of neurological diseases in humans.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Enzymes that catalyze the formation of acyl-CoA derivatives. EC 6.2.1.
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
A status with BODY WEIGHT that is grossly above the acceptable or desirable weight, usually due to accumulation of excess FATS in the body. The standards may vary with age, sex, genetic or cultural background. In the BODY MASS INDEX, a BMI greater than 30.0 kg/m2 is considered obese, and a BMI greater than 40.0 kg/m2 is considered morbidly obese (MORBID OBESITY).
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A doubly unsaturated fatty acid, occurring widely in plant glycosides. It is an essential fatty acid in mammalian nutrition and is used in the biosynthesis of prostaglandins and cell membranes. (From Stedman, 26th ed)
Regular course of eating and drinking adopted by a person or animal.
The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.
Proteins which bind with RETINOL. The retinol-binding protein found in plasma has an alpha-1 mobility on electrophoresis and a molecular weight of about 21 kDa. The retinol-protein complex (MW=80-90 kDa) circulates in plasma in the form of a protein-protein complex with prealbumin. The retinol-binding protein found in tissue has a molecular weight of 14 kDa and carries retinol as a non-covalently-bound ligand.
Elements of limited time intervals, contributing to particular results or situations.
UNSATURATED FATTY ACIDS that contain at least one double bond in the trans configuration, which results in a greater bond angle than the cis configuration. This results in a more extended fatty acid chain similar to SATURATED FATTY ACIDS, with closer packing and reduced fluidity. HYDROGENATION of unsaturated fatty acids increases the trans content.
Eighteen-carbon essential fatty acids that contain two double bonds.
Glucose in blood.
One of the three polypeptide chains that make up the TROPONIN complex. It inhibits F-actin-myosin interactions.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A 16-kDa peptide hormone secreted from WHITE ADIPOCYTES. Leptin serves as a feedback signal from fat cells to the CENTRAL NERVOUS SYSTEM in regulation of food intake, energy balance, and fat storage.
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A class of organic compounds which contain an anilino (phenylamino) group linked to a salt or ester of naphthalenesulfonic acid. They are frequently used as fluorescent dyes and sulfhydryl reagents.
An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Important polyunsaturated fatty acid found in fish oils. It serves as the precursor for the prostaglandin-3 and thromboxane-3 families. A diet rich in eicosapentaenoic acid lowers serum lipid concentration, reduces incidence of cardiovascular disorders, prevents platelet aggregation, and inhibits arachidonic acid conversion into the thromboxane-2 and prostaglandin-2 families.
THIAZOLES with two keto oxygens. Members are insulin-sensitizing agents which overcome INSULIN RESISTANCE by activation of the peroxisome proliferator activated receptor gamma (PPAR-gamma).
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Salts and esters of the 18-carbon saturated, monocarboxylic acid--stearic acid.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A subclass of retinol-binding proteins that take part in the intracellular storage and transport of RETINOL. They are both functionally and structurally distinct from PLASMA RETINOL-BINDING PROTEINS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Oils high in unsaturated fats extracted from the bodies of fish or fish parts, especially the LIVER. Those from the liver are usually high in VITAMIN A. The oils are used as DIETARY SUPPLEMENTS. They are also used in soaps and detergents and as protective coatings.
A family of immunophilin proteins that bind to the immunosuppressive drugs TACROLIMUS (also known as FK506) and SIROLIMUS. EC 5.2.1.-
A subclass of DIABETES MELLITUS that is not INSULIN-responsive or dependent (NIDDM). It is characterized initially by INSULIN RESISTANCE and HYPERINSULINEMIA; and eventually by GLUCOSE INTOLERANCE; HYPERGLYCEMIA; and overt diabetes. Type II diabetes mellitus is no longer considered a disease exclusively found in adults. Patients seldom develop KETOSIS but often exhibit OBESITY.
High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
C22-unsaturated fatty acids found predominantly in FISH OILS.
Compounds that contain a 1-dimethylaminonaphthalene-5-sulfonyl group.
A sialic acid binding lectin that was originally identified as an adhesion molecule for inflammatory MACROPHAGES and activated MONOCYTES. This protein is the largest known siglec subtype and contains 16 immunoglobulin C2-set domains. It plays a role in cell to cell interactions and interactions with BACTERIA.
A poly(A) binding protein that has a variety of functions such as mRNA stabilization and protection of RNA from nuclease activity. Although poly(A) binding protein I is considered a major cytoplasmic RNA-binding protein it is also found in the CELL NUCLEUS and may be involved in transport of mRNP particles.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A benzofuran derivative used as a protein reagent since the terminal N-NBD-protein conjugate possesses interesting fluorescence and spectral properties. It has also been used as a covalent inhibitor of both beef heart mitochondrial ATPase and bacterial ATPase.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
A family of soluble proteins that bind insulin-like growth factors and modulate their biological actions at the cellular level. (Int J Gynaecol Obstet 1992;39(1):3-9)
Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
A family of SIALIC ACID binding proteins found in vertebrate species. They are transmembrane proteins which act as cell surface receptors for a variety of sialylated GLYCOCONJUGATES. While a subset of siglec protein subtypes are evolutionarily conserved between mammalian species, there are many others that are species specific.
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Maleness or femaleness as a constituent element or influence contributing to the production of a result. It may be applicable to the cause or effect of a circumstance. It is used with human or animal concepts but should be differentiated from SEX CHARACTERISTICS, anatomical or physiological manifestations of sex, and from SEX DISTRIBUTION, the number of males and females in given circumstances.
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.

A concise promoter region of the heart fatty acid-binding protein gene dictates tissue-appropriate expression. (1/1344)

The heart fatty acid-binding protein (HFABP) is a member of a family of binding proteins with distinct tissue distributions and diverse roles in fatty acid metabolism, trafficking, and signaling. Other members of this family have been shown to possess concise promoter regions that direct appropriate tissue-specific expression. The basis for the specific expression of the HFABP has not been previously evaluated, and the mechanisms governing expression of metabolic genes in the heart are not completely understood. We used transient and permanent transfections in ventricular myocytes, skeletal myocytes, and nonmyocytic cells to map regulatory elements in the HFABP promoter, and audited results in transgenic mice. Appropriate tissue-specific expression in cell culture and in transgenic mice was dictated by 1.2 kb of the 5'-flanking sequence of FABP3, the HFABP gene. Comparison of orthologous murine and human genomic sequences demonstrated multiple regions of near-identity within this promoter region, including a CArG-like element close to the TATA box. Binding and transactivation studies demonstrated that this element can function as an atypical myocyte enhancer-binding factor 2 site. Interactions with adjacent sites are likely to be necessary for fully appropriate, tissue-specific, developmental and metabolic regulation.  (+info)

Variation of liver-type fatty acid binding protein content in the human hepatoma cell line HepG2 by peroxisome proliferators and antisense RNA affects the rate of fatty acid uptake. (2/1344)

The liver-type fatty acid binding protein (L-FABP), a member of a family of mostly cytosolic 14-15 kDa proteins known to bind fatty acids in vitro and in vivo, is discussed to play a role in fatty acid uptake. Cells of the hepatoma HepG2 cell line endogenously express this protein to approximately 0.2% of cytosolic proteins and served as a model to study the effect of L-FABP on fatty acid uptake, by manipulating L-FABP expression in two approaches. First, L-FABP content was more than doubled upon treating the cells with the potent peroxisome proliferators bezafibrate and Wy14,643 and incubation of these cells with [1-14C]oleic acid led to an increase in fatty acid uptake rate from 0.55 to 0.74 and 0.98 nmol/min per mg protein, respectively. In the second approach L-FABP expression was reduced by stable transfection with antisense L-FABP mRNA yielding seven clones with L-FABP contents ranging from 0.03% to 0.14% of cytosolic proteins. This reduction to one sixth of normal L-FABP content reduced the rate of [1-14C]oleic acid uptake from 0.55 to 0. 19 nmol/min per mg protein, i.e., by 66%. The analysis of peroxisome proliferator-treated cells and L-FABP mRNA antisense clones revealed a direct correlation between L-FABP content and fatty acid uptake.  (+info)

Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells. (3/1344)

Epidermal growth factor (EGF) has been reported to stimulate carbohydrate, amino acid, and electrolyte transport in the small intestine, but its effects on lipid transport are poorly documented. This study aimed to investigate EGF effects on fatty acid uptake and esterification in a human enterocyte cell line (Caco-2). EGF inhibited cell uptake of [14C]palmitate and markedly reduced its incorporation into triglycerides. In contrast, the incorporation in phospholipids was enhanced. To elucidate the mechanisms involved, key steps of lipid synthesis were investigated. The amount of intestinal fatty acid-binding protein (I-FABP), which is thought to be important for fatty acid absorption, and the activity of diacylglycerol acyltransferase (DGAT), an enzyme at the branch point of diacylglycerol utilization, were reduced. EGF effects on DGAT and on palmitate esterification occurred at 2-10 ng/ml, whereas effects on I-FABP and palmitate uptake occurred only at 10 ng/ml. This suggests that EGF inhibited palmitate uptake by reducing the I-FABP level and shifted its utilization from triglycerides to phospholipids by inhibiting DGAT. This increase in phospholipid synthesis might play a role in the restoration of enterocyte absorption function after intestinal mucosa injury.  (+info)

Fatty acid binding protein in heart and skeletal muscles of the migratory barnacle goose throughout development. (4/1344)

The long-distance migratory flights of birds are predominantly fueled by the oxidation of fatty acids, which are sourced primarily from extracellular adipose stores. These fatty acids have to be transported, via the circulatory system, to the mitochondria of the active muscles. An important facilitator of fatty acid transport within the cytoplasm of muscle cells is fatty acid binding protein (FABP), which serves as an intracellular carrier of long-chain fatty acids. In mammals, the muscular FABP content is related to the fatty acid oxidation capacity of the tissue. The aim of this study was to measure FABP in samples taken from the cardiac, pectoralis, and semimembranosus muscles of a long-distance avian migrant, the barnacle goose (Branta leucopsis), at various stages of development. Western blot analysis identified a single goose muscle protein of 15 kDa that was able to bind fatty acids and showed a 66% cross-reactivity with antibodies against human heart-type FABP. Captive goslings showed no significant changes in FABP content of either the heart (62.6 +/- 10.6 microgram/g wet wt) or the semimembranosus muscle (8.4 +/- 1.9 microgram/g wet wt) during development. However, in both peripheral and deep sites within the pectoralis muscle, FABP content of samples taken from captive goslings were approximately 10-fold higher throughout development and reached values of 30-40 microgram/g wet wt in fledging goslings at 7 wk of age. A further twofold higher value was seen in wild but not in captive goslings immediately before migration (12 wk of age). Similarly, FABP content was significantly higher in pectoralis samples taken from wild adults (94.3 +/- 3.6 microgram/g wet wt) compared with those from captive adults (60.5 +/- 3.6 micro/g wet wt). These results suggest that the experience of flight activity may be of critical importance in achieving maximal expression of FABP in the pectoralis muscles of postfledging and mature geese immediately before migration.  (+info)

Differential expression of AP-2alpha and AP-2beta in the developing chick retina: repression of R-FABP promoter activity by AP-2. (5/1344)

Retinal fatty acid binding protein (R-FABP) is the avian counterpart of murine brain FABP implicated in glial cell differentiation and neuronal cell migration. R-FABP is highly expressed in the undifferentiated retina and brain of chick embryos. We have previously shown by in vitro studies that the AP-2 transcription factor binds to a consensus AP-2 binding site in the R-FABP promoter region. Based on the expression pattern of AP-2 in the developing retina and on mutational analysis of the AP-2 binding site in DNA transfection experiments, we proposed that AP-2 could be involved in the down-regulation of R-FABP transcription. Here, we describe the cDNA isolation of two members of the AP-2 family expressed in the chick retina, AP-2alpha and AP-2beta. We show that R-FABP mRNA and the AP-2 factors are expressed in mutually exclusive patterns in the differentiating retina: whereas AP-2alpha and AP-2beta are selectively expressed either in amacrine, or in amacrine and horizontal cells, respectively, R-FABP mRNAis found in Muller glial cells and/or bipolar cells. Furthermore, a decrease in R-FABP-dependent expression is obtained upon cotransfection of primary retinal cultures with AP-2 expression vectors and a CAT reporter construct. The early and cell-specific expression of AP-2alpha and AP-2beta in the developing retina suggest a role for this transcription factor family in the early steps of amacrine and horizontal cell differentiation. Repression of the R-FABP gene in these cells may be an important component of their developmental program.  (+info)

Analysis of the fatty acid components in a perchloric acid-soluble protein. (6/1344)

We had previously found that a perchloric acid-soluble protein (PSP1) occurs in rat liver, and that this novel protein inhibits protein synthesis in a rabbit reticulocyte lysate system (T. Oka, H. Tsuji, C. Noda, K. Sakai, Y.-H. Hong, I. Suzuki, S. Munoz, Y. Natori, J. Biol. Chem. 270 (1995) 30060-30067). In the present study, we analyzed lipid components bound to PSP1. Native PSP1 was purified from rat liver using Sephadex G-75, DE-52 cellulose and IgGPSP-affinity chromatography, and the lipid components were extracted. The components obtained from the purified PSP1 were shown to be free fatty acids by thin-layer chromatography. By GC-MS, six major fatty acids were identified as 14:0, 16:0, 18:0, 18:1, 18:2 and 20:4. 1 mol of PSP1 contained 1.26 mol of total fatty acid components. The fatty acid-binding assay of PSP1 showed that the Bmax was 1.25 mol fatty acid/mol PSP1 and the Kd value for palmitic acid was 6.03 microM. The concentration of PSP1 mRNA in rat liver increased 2.3-fold by the administration of peroxisome proliferator, bezafibrate. These findings show that PSP1 is a fatty acid-binding protein-like protein, which is involved in the intracellular metabolism of fatty acid and is quite different from the known fatty acid-binding proteins.  (+info)

Calcium-binding protein S100A7 and epidermal-type fatty acid-binding protein are associated in the cytosol of human keratinocytes. (7/1344)

Expression of epidermal-type fatty acid-binding protein (E-FABP) and S100A7 has previously been shown to be elevated in psoriatic skin, a disease characterized by abnormal keratinocyte differentiation. However, no causal relationship between the up-regulation of these proteins and the disease has been shown. E-FABP is thought to be involved in cytosolic fatty acid (FA) transport, whereas the role of S100A7 is still unknown. In this report, we show by overlay assays that E-FABP, immobilized on nitrocellulose, is able to capture S100A7 from cytosolic psoriatic protein extracts and vice versa, suggesting the formation of a complex between the two proteins. Using purified E-FABP and S100A7, the complex can be reconstituted only in presence of EDTA. Moreover, we show that increased EDTA concentrations in psoriatic cytosolic protein extracts enhance complex formation. Partial complex disruption was obtained by the addition of physiological concentrations of Zn2+ (0.1 mM), whereas Ca2+ at 5 mM and Mg2+ at 30 mM had no effect. On the other hand, high Ca2+ concentrations (30 mM) resulted in partial complex disruption. Oleic acid-binding properties were observed for free E-FABP and the complex E-FABP-S100A7, but not for free S100A7. By using confocal microscopy we show that S100A7 and E-FABP are co-localized in the cytoplasm of differentiating keratinocytes from lesional psoriatic skin. These data indicate that formation of the E-FABP-S100A7 complex and its FA-binding function might be regulated at least by bivalent cations.  (+info)

Phytanic acid is ligand and transcriptional activator of murine liver fatty acid binding protein. (8/1344)

Branched-chain phytanic acid is metabolized in liver peroxisomes. Sterol carrier protein 2/sterol carrier protein x (SCP2/SCPx) knockout mice, which develop a phenotype with a deficiency in phytanic acid degradation, accumulate dramatically high concentrations of this fatty acid in serum (Seedorf at al. 1998. Genes Dev. 12: 1189-1201) and liver. Concomitantly, a 6.9-fold induction of liver fatty acid binding protein (L-FABP) expression is observed in comparison to wild-type animals fed standard chow, possibly mediated by the peroxisome proliferator-activated receptor alpha (PPARalpha). Cytosolic transport of phytanic acid to either peroxisomal membranes or to the nucleus for activation of PPARalpha may be mediated by L-FABP, which gives rise to the question whether phytanic acid is a transactivator of this protein. Here we show first that phytanic acid binds to recombinant L-FABP with high affinity. Then the increase of the in vivo phytanic acid concentration by phytol feeding to mice results in a 4-fold induction of L-FABP expression in liver, which is in the order of that attained with bezafibrate, a known peroxisome proliferator. Finally to test in vitro whether this induction is conferred by phytanic acid, we cotransfected HepG2 cells with an expression plasmid for murine PPARalpha and a CAT-reporter gene with 176 bp of the murine L-FABP promoter, containing the peroxisome proliferator responsive element (PPRE). After incubation with phytanic acid, we observed a 3.2-fold induction of CAT expression. These findings, both in vivo and in vitro, demonstrate that phytanic acid is a transcriptional activator of L-FABP expression and that this effect is mediated via PPARalpha.  (+info)

Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. The HADHB gene is located on chromosome 1, with its specific location being 1p33-p32. The gene contains 5 exons. HADHB encodes a 51.2 kDa protein that is composed of 133 amino acids; 124 peptides have been observed through mass spectrometry data. Heart-type Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. Like the nine other distinct FABPs that have been identified, H-FABP is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. See FABP3 for biochemical details. The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are ...
Introduction: Biomarkers would be helpful for early prognosis in out-of-hospital cardiac arrest (OHCA) patients. Heart-type fatty acid binding protein (H-FABP) is a tissue-specific protein which is rapidly released into the circulation when the cardiomyocyte injuries happen. The role of circulating H-FABP level for outcomes remains unclear in OHCA patients.. Hypothesis: The higher plasma level of H-FABP is associated with worse outcome in OHCA patients.. Methods: The study was a prospectively cohort study enrolling non-traumatic resuscitated OHCA patients in a tertiary medical center. All of the cardiac arrest event variables were recorded according to the Utstein style recommendations. Blood samples were collected at 24 hours after cardiac arrest and resuscitation. The primary endpoint was defined as the survival to discharge. The secondary endpoint was neurological status at time point of hospital discharge evaluated by cerebral performance category (CPC) score and survival to 90 ...
The association between ongoing myocardial damage and outcomes in patients who have received an implantable cardioverter-defibrillator (ICD) is unclear. Consecutive patients with cardiomyopathy, who had received an ICD (n = 107, mean age 65 ±
To the Editor We read with interest the systematic review and meta-analysis by Bruins Slot et al concerning early diagnosis of myocardial infarction (MI) using heart-type fatty acid-binding protein (H-FABP).1 We agree with their summary that when used in isolation, H-FABP may not offer a diagnostic advantage over the current troponin standard. However, it should be noted that in five of the included studies constituting 1573 patients (42% of the pooled cohort), no … ...
Fingerprint Dive into the research topics of Heart-type fatty acid binding protein is an independent predictor of death and ventricular dysfunction after coronary artery bypass graft surgery. Together they form a unique fingerprint. ...
Li, H. [李慧颖]. (2010). Adipocyte fatty acid-binding protein : a link between inflammation and vascular dysfunction. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_ ...
TY - JOUR. T1 - Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages. AU - Shores, Darla R.. AU - Fundora, Jennifer. AU - Go, Mitzi. AU - Shakeel, Fauzia. AU - Brooks, Sandra. AU - Alaish, Samuel M.. AU - Yang, Jun. AU - Sodhi, Chhinder P.. AU - Hackam, David J.. AU - Everett, Allen. N1 - Publisher Copyright: © 2020 The Author(s).. PY - 2020/5/26. Y1 - 2020/5/26. N2 - Background: Necrotizing enterocolitis (NEC) is associated with significant morbidity and mortality. Serum biomarkers to aid diagnosis, such as intestinal fatty acid binding protein (IFABP) and calprotectin, are actively being investigated; however, the normative values of these markers among healthy premature and term infants remains unknown. We sought to identify normative values for the serum concentrations of IFABP and calprotectin across gestational (GA) and post-menstrual age. Methods: We collected serum from infants (24-40 weeks GA) in the first week of life and at ...
Fatty acid binding protein 6, ileal (gastrotropin), also known as FABP6, is a protein which in humans is encoded by the FABP6 gene. This gene encodes the ileal fatty acid binding protein. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP6 and FABP1 (the liver fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Transcript variants generated by alternate transcription promoters and/or alternate splicing have been found for this gene. GRCh38: Ensembl release 89: ENSG00000170231 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000020405 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: FABP6 fatty acid binding protein 6, ileal (gastrotropin). Börchers T, Hohoff C, Buhlmann C, Spener F (1997). Heart-type fatty acid binding protein - involvement in ...
This 10-year prospective study in 1069 patients with stable CHD at baseline provides the longest observational clinical evidence so far available for serum A-FABP and underlines that this circulating protein may be an important pathoyphysiological mediator of future cardiovascular morbidity and mortality in individuals with manifest atherosclerosis. In this population, increased serum A-FABP levels were also associated with metabolic and inflammatory cardiovascular risk factors at baseline, including adiposity, atherogenic dyslipidemia, hyperglycemia, hypertension, hs-CRP, and IL-6. During the long-term follow-up, A-FABP significantly predicted major secondary CVD events (cardiovascular death, nonfatal MI, or ischemic stroke) in crude analyses and for the most impactful outcome of cardiovascular death even after adjustment for established cardiovascular risk factors and lipid-lowering drugs. These findings suggest an independent proatherogenic role of this protein in serum, thereby expanding ...
1ICM: Escherichia coli-derived rat intestinal fatty acid binding protein with bound myristate at 1.5 A resolution and I-FABPArg106-->Gln with bound oleate at 1.74 A resolution.
Results The primary outcome measure of death or readmission with myocardial infarction after a minimum follow-up period of 12 months (median 18 months) occurred in 96 of 955 patients (10.1%). The H-FABP concentration was an independent predictor of death or myocardial infarction, after multivariate adjustment. Patients with H-FABP concentrations ,6.48 μg/l had significantly increased risk of adverse events (adjusted hazard ratio: 2.62, 95% confidence interval: 1.30 to 5.28, p = 0.007). Among troponin-negative patients (which constituted 79.2% of the cohort), the aforementioned cutoff of 6.48 μg/l identified patients at very high risk for adverse outcomes independent of patient age and serum creatinine. ...
TY - JOUR. T1 - Dynamics of internal water in fatty acid binding protein. T2 - Computer simulations and comparison with experiments. AU - Likić, Vladimir A.. AU - Prendergast, Franklyn G.. PY - 2001/4/1. Y1 - 2001/4/1. N2 - Multiple molecular dynamics (MD) simulations of fully solvated rat intestinal fatty acid binding protein (I-FABP) were conducted to investigate the dynamics of internal water molecules. Although the long time average of the number of internal water molecules in I-FABP is 22 as shown by the X-ray crystal structure, MD simulations predict large variations in the instantaneous number of internal water molecules on the nanosecond time scale. The computational model employed predicts that w135 (internal) and w217 (located on the protein surface) may be the water molecules with long residence times observed in previously reported magnetic relaxation dispersion studies. The average residence time of ∼20 internal water molecules occupying the fatty acid binding cavity is estimated ...
Alias: Fabph1, Fabph-1, Fabph4, Fabph-4, Fatty acid-binding protein, heart, Fatty acid-binding protein 3, Heart-type fatty acid-binding protein, H-FABP, Mammary-derived growth inhibitor, Mdgi, MDGI ...
Mammalian intestinal fatty acid-binding protein (I-FABP) is a small cytosolic protein and is thought to play a crucial role of intracellular fatty acid trafficking and metabolism in gut. To establish an in vivo system for investigating its tissue-specific regulation during zebrafish intestinal devel …
TY - GEN. T1 - Heart fatty-acid binding protein gene variants are associated with intramuscular fat content and back fat thickness in pigs. AU - Gerbens, F.. AU - van Erp, A.J.M.. AU - Meuwissen, T.H.E.. AU - Veerkamp, J.H.. AU - te Pas, M.F.W.. PY - 1998. Y1 - 1998. M3 - Conference paper. SP - 187. EP - 190. BT - Proc. 6th world congress on genetics applied to livestock production 26, 1998. ER - ...
SummaryThe main objective was to evaluate the association between SNPs and haplotypes of the FABP1-4 genes and type 2 diabetes, as well as its interaction with fat intake, in one general Spanish population. The association was replicated in a second population in which HOMA index was also evaluated.Methods1217 unrelated individuals were selected from a population-based study [Hortega study: 605 women; mean age 54 y; 7.8% with type 2 diabetes]. The replication population included 805 subjects from Segovia, a neighboring region of Spain (446 females; mean age 52 y; 10.3% with type 2 diabetes). DM2 mellitus was defined in a similar way in both studies. Fifteen SNPs previously associated with metabolic traits or with potential influence in the gene expression within the FABP1-4 genes were genotyped with SNPlex and tested. Age, sex and BMI were used as covariates in the logistic regression model.ResultsOne polymorphism (rs2197076) and two haplotypes of the FABP-1 showed a strong association with the ...
F0019-75W1 -500ul : Fatty Acid Binding Protein, Heart (Heart-type Fatty Acid-binding Protein, H-FABP, Fatty Acid Binding Protein 3, FABP3, FABP11, Mammary-derived Growth Inhibitor, MDGI, Muscle Fatty Acid Binding Protein, M-FABP, O-FABP) (Biotin ...
Introduction: Ischemic stroke is associated with gastrointestinal complications such as constipation, dysphagia and fecal incontinence. However, the precise mechanisms involved have not been fully elucidated. D-Lactate and Intestinal Fatty-Acid Binding Protein (IFABP) are markers of intestinal mucosa integrity and barrier function. We evaluated serum concentrations of these markers in patients with acute ischemic stroke.. Materials and methods: We included consecutive patients with ischemic stroke, and used healthy, age and sex-matched subjects as controls. We excluded cases determined to be of other or undetermined etiology according to the TOAST classification. Blood was drawn within 24 hours of symptom onset. Serum concentrations of D-Lactate and IFABP were determined using commercially available colorimetric and ELISA kits, respectively.. Results: We included a total of 61 patients and 20 controls. The majority of patients were male (57.4%), and had a median age of 64 years. The most common ...
TY - JOUR. T1 - Serum concentrations of myoglobin vs human heart-type cytoplasmic fatty acid-binding protein in early detection of acute myocardial infarction. AU - Ishii, J.. AU - Wang, J.. AU - Naruse, H.. AU - Taga, S.. AU - Kinoshita, M.. AU - Kurokawa, H.. AU - Iwase, M.. AU - Kondo, T.. AU - Nomura, M.. AU - Nagamura, Y.. AU - Watanabe, Y.. AU - Hishida, H.. AU - Tanaka, T.. AU - Kawamura, K.. PY - 1997/8/19. Y1 - 1997/8/19. N2 - We compared the diagnostic utility of serum concentrations of human heart-type cytoplasmic fatty acid-binding protein (H-FABPc), myoglobin, and their ratio for the early diagnosis of acute myocardial infarction (AMI) in 104 healthy volunteers and 165 patients at admission within 6 h of the onset of chest pain. The ROC curves of the H-FABPc [0.946, 95% confidence interval (CI) = 0.913-0.979] and myoglobin (0.895, 95% CI = 0.8460.944) between patients with AMI and healthy volunteers were significantly greater than the area under the ratio of myoglobin to H-FABPc ...
BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is a candidate tumor suppressor gene for human breast cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016 ...
Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, BIOCHEMISTRY & MOLECULAR BIOLOGY, fatty acid-binding protein, FABP, liver, intestine, FLUORESCENT-PROBE ADIFAB, HEART, TRANSPORT, LIGAND, ADIPOCYTE, EXCHANGE, VESICLES ...
15 Jan 2013 New paper in Medical Decision Making by Mark Strong and Jeremy Oakley on fast efficient computation of partial EVPI. See publications page. 3 August 2012 Manuscript discussing the cost-effectiveness of presentation versus delayed troponin testing for acute myocardial infarction published in Heart. 29 May 2012 Registration still open for the Uncertainty in Computer Models 2012 Conference, July 2-4, 2012, Sheffield, UK. 16 May 2012 Manuscript discussing heart-type fatty acid binding protein as an early marker for myocardial infarction: systematic review and meta-analysis published online in Emerging Medicine Journal. 5 April 2012 Manuscript assessing the cost-effectiveness of presentation and delayed troponin testing for acute myocardial infarction submitted to Heart. 3 February 2012 Manuscript discussing the cost-effectiveness of cilostazol, naftidrofuryl oxalate, pentoxifylline and inositol nicotinate for the treatment of intermittent claudication in people with peripheral arterial ...
F0019-90C -50ug : FABP7, CT (Fatty Acid-binding Protein, Brain, Brain Lipid-binding Protein, BLBP, Brain-type Fatty Acid-binding Protein, B-FABP, Fatty Acid-binding Protein 7, Mammary-derived Growth Inhibitor Related, BLBP, FABPB, MRG ...
Cigarette smoking causes a decrease in the glomerular filtration rate in diabetic patients with normal renal function, independent of confounding factors including severe proteinuria (1). It increases the risk of microalbuminuria and accelerates the progression from microalbuminuria to overt proteinuria as well as the progression of renal failure in patients with type 2 diabetes (2). It is widely accepted that the rate of functional decline correlates with the degree of renal tubulointerstitial fibrosis. Previous studies have shown that renal function in patients with type 2 diabetes correlates better with tubular changes than with glomerular pathology (3). Further studies on tubulointerstitial injury in patients with diabetic nephropathy may provide additional insight into the pathogenesis of diabetic nephropathy and lead to the identification of therapeutic targets. Liver-type fatty acid-binding protein (l-FABP) is expressed in the proximal tubules, where it plays a key role in fatty acid ...
Peroxisome proliferator-activated receptors (PPARα, PPARβ, and PPARγ) are transcription factors activated by fatty acids and some antidiabetogenic drugs. Wolfrum et al. identified liver fatty acid binding protein (LFABP) as a protein that binds to the same ligands as does PPARα and that interacts directly with PPARα and PPARγ. They propose that LFABP, which can be found in both the cytoplasm and the nucleus, acts as a shuttle to deliver the PPAR agonists to the nuclear-localized PPAR receptors. In support of this model, transactivation of a PPAR reporter construct increased linearly with the amount of LFABP expressed.. C. Wolfrum, C. M. Borrmann, T. Börchers, F. Spener, Fatty acids and hypolipidemic drugs regulate peroxisome proliferator-activated receptors α- and γ-mediated gene expression via liver fatty acid binding protein: A signaling path to the nucleus. Proc. Natl. Acad. Sci. U.S.A. 98, 2323-2328 (2001). [Abstract] [Full Text]. ...
Science & Technology, Life Sciences & Biomedicine, Cell Biology, CELL BIOLOGY, BODIPY, fatty acid-binding protein, FABP, fluorescence, microscopy, CELL-LINE CACO-2, SPECTRAL PROPERTIES, CRYSTAL-STRUCTURE, LIVER, TRAFFICKING, ENDOCYTOSIS, INTESTINE, TRANSPORT, MEMBRANES, PROBES ...
How is Fatty Acid Binding protein 2 abbreviated? FABP2 stands for Fatty Acid Binding protein 2. FABP2 is defined as Fatty Acid Binding protein 2 frequently.
Insulin resistance is a characteristic feature in FCHL.9 10 Since genetic variation in the FABP2 gene could be the cause of insulin resistance, we screened for variants in this gene in patients with FCHL. According to our study, the FABP2 gene is not likely to be a major gene for FCHL. However, the polymorphism in codon 54 of this gene (Ala→Thr) may contribute to abnormalities in lipid metabolism in FCHL, although it does not have a significant effect on insulin resistance.. Complex segregation analysis has suggested a major gene effect on apoB28 and on triglycerides29 in FCHL. Although some uncommon variants in the lipoprotein lipase gene26 could cause FCHL, no gene that could explain a major part of this disease has yet been found. We found one novel nucleotide substitution in the 3′ noncoding region (A→G) of the FABP2 gene in patients with FCHL. In addition, we found nucleotide substitutions in codon 54 (GCT→ACT) and in codon 118 (GTA→GTG) described previously in Pima Indians.13 ...
0011] FIG. 1. (A) Schematic of the transgene vector (10871 bp) with porcine CFTR cDNA (1790-6238) driven by the rat intestinal fatty acid binding protein (iFABP) promoter (209-1419), flanked by the intervening sequence and the bovine growth hormone poly-A (BGHpA) (6295-6508), and followed by a hygromycin cDNA sequence (7392-8415) flanked by SV40 (7049-7373) and SV40 poly-A (8428-8800) signal sequences. Bgl I restriction site is denoted. (B) Gross image of a meconium plug (green-black colored portion of stool) that was passed by a CFTR-/-; Tg-FABP-pCFTR pig (line 1e) following an enema at approximately 18 h after birth. After the meconium plug was passed, a transition to normal-appearing stool was observed (yellow-green stool denoted by white arrows) bar=2 em. (C) Gross images from the gastrointestinal tract of CFTR-/-; Tg-FABP-pCFTR piglets. At birth, in 2 of the 5 lines meconium ileus was alleviated in CFTR-/-; Tg-FABP-pCFTR pigs. Meconium ileus lesions ranged from absent (left panel, line 1a) ...
Results: Liver fatty acid binding protein mRNA and protein levels were reduced in the liver of animals with fatty liver disease when compared to controls. Increased hepatic fat content was accompanied by higher levels of oxidative stress in animals with fatty liver disease when compared to controls. An inverse association was found between the levels of hepatic liver fatty acid binding protein and the level of hepatic oxidative stress in fatty liver disease. Elevated NADPH oxidase protein levels were detected in the liver of animals with increased severity in inflammation and fibrosis. Cocoa supplementation was associated with partial attenuation of these pathological changes, although the severity of liver disease induced by the methionine choline deficient diet prevented complete reversal of any disease associated changes. Red blood cell glutathione was increased by cocoa supplementation, whereas liver glutathione was reduced by cocoa compared to methionine choline deficient diet fed animals ...
250 µCi quantities of [1-14C]-Oleic Acid are available for your research. Application of [14C]Oleic Acid can be found in: trans-10, cis-12 conjugated linoleic acid decreasing de novo lipid synthesis in human adipocytes, effect of dietary phosphatidylcholine on the assimilation and distribution of ingested free oleic acid in aquaculture, variation of liver-type fatty acid binding protein content in the human HepG2 affecting the rate of fatty acid uptake, oleic acid stimulating rapid translocation of cholinephosphate cytidylyltransferase in type II cells, intestinal-fatty acid binding protein (I-FABP) and lipid transport in human intestinal epithelial cells, etc. ...
TY - GEN. T1 - A Multi-Stage decision support Algorithm to Rule-Out patientswith suspected Acute Myocardial Infarction (AMI). AU - Navarro-Paredes, Cesar. AU - Shand, James A. AU - McEneaney, David. AU - McLaughlin, James. PY - 2016/5/27. Y1 - 2016/5/27. N2 - Objective: Provide a multi-stage rule-out algorithm to stratify patients admitted to the Emergency Room (ER) with chest pain of presumed ischemic origin. The aim is to keep at-risk patients in the ER providing a proper care while minimizing overcrowding. The algorithm uses data from biomarkers -heart-type fatty acid-binding protein (H-FABP), high sensitivity cardiac troponin T (hs-cTnT) measured at different times (presentation, 1, 2, 3, 6, 12 and 24 hours) together with ECG at presentation.Methods: Data in a randomly selected training set of 296 patients were retrospectively analysed. 182 cases comprised a test set. STEMI were not considered since biomarkers are not routinely measured for these cases. H-FABP and hs-cTnT were statistically ...
Mice null for adipocyte fatty acid binding protein (AFABP) compensate by increasing expression of keratinocyte fatty acid binding protein (KFABP) (Hotamisligil et al. Science 274:1377-1379, 1996). In the present study, AFABP knockout (KO) and wild-type (WT) mice became equally obese on a high-fat diet, as judged by fat pad weights, adipocyte size, and body composition analysis. High-fat feeding led to moderate insulin resistance in both WT and AFABP knockout mice, as indicated by an approximately 2-fold increase in plasma insulin. However, in the high fat-fed mice, plasma glucose levels were approximately 15% lower in the AFABP-KO mice. Adipocytes isolated from AFABP-KO and WT mice fed high- or low-fat diets exhibited similar rates of basal and norepinephrine-stimulated lipolysis and insulin-stimulated rates of glucose conversion to fatty acids and glyceride-glycerol. However, basal glucose conversion to fatty acids was higher in adipocytes of AFABP-KO mice. Adipocyte tumor necrosis factor-alpha ...
1AEL: Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.
In this study, we examined the relationship between severity of CAD and heart-type fatty acid-binding protein (H-FABP), a new marker of ischemia in patients with non-ST-segment elevation acute ... Archives of the Turkish Society of Cardiology, Zeren, Gé° é»®, Erer, Hatice Beté»®, Ké½¬î §, Tuncay, 轪hin, Osman, Aksu, H黶eyin, Ké° r黮�, Diyar, G黺en�, ... ...
Self-association of the cardiac fatty acid-binding protein. Influence on membrane-bound, fatty-acid-dependent enzymes: Biochemistry
glucosaminidase (NAG) are potentially useful biomarkers for the diagnosis of established AKI. Urinary NGAL, IL-18, and liver-type fatty acid binding protein, and serum NGAL and cystatin C represent the most promising biomarkers for early prediction of AKI. Urinary cystatin C, 1-microglobulin, NAG and retinol-binding protein may be useful to predict severity and outcomes of AKI. In conclusion, we identified several studies of promising biomarkers for the diagnosis, prediction and prognostication of AKI. However, we note several limitations, including small sample sizes, inadequate gold standard, exclusion of patients with chronic kidney disease, incomplete statistical analyses, utilization of research-based assays and a paucity of studies examining prediction for clinical outcomes. Future studies will need to address these limitations in order for further progress to be made.. ...
The Adipocyte Fatty Acid-Binding Protein (AFABP) is mainly expressed in fat cells. It can bind fatty acids and other lipophilic substances such as eicosanoids and retinoids. The peroxisome proliferator-activated receptor γ (PPARγ) is a nuclear receptor protein that requires ligand binding to regulate the specific gene transcription. PPARγ is expressed at extremely high levels in adipose tissue, macrophages, and the large intestine, where it controls lipid adipogenesis and energy conversion. Moreover, it has been found that AFABP and PPARγ can form a complex in vivo. It is proposed that AFABP carries the ligand and enters into the nucleus where it transfers the ligand to PPARγ by binding and macromolecular interaction. The goal of this project is to study the interaction between these two proteins in vitro, including their binding affinity, the location of the binding interface on the AFABP protein, and the dependence of these phenomena on specific ligands. New protocols were developed to obtain
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is ...
TY - JOUR. T1 - Mutations in Ribonucleic Acid Binding Protein Gene Cause Familial Dilated Cardiomyopathy. AU - Brauch, Katharine M.. AU - Karst, Margaret L.. AU - Herron, Kathleen J.. AU - de Andrade, Mariza. AU - Pellikka, Patricia A.. AU - Rodeheffer, Richard J.. AU - Michels, Virginia V.. AU - Olson, Timothy M.. PY - 2009/9/1. Y1 - 2009/9/1. N2 - Objectives: We sought to identify a novel gene for dilated cardiomyopathy (DCM). Background: DCM is a heritable, genetically heterogeneous disorder that remains idiopathic in the majority of patients. Familial cases provide an opportunity to discover unsuspected molecular bases of DCM, enabling pre-clinical risk detection. Methods: Two large families with autosomal-dominant DCM were studied. Genome-wide linkage analysis was used to identify a disease locus, followed by fine mapping and positional candidate gene sequencing. Mutation scanning was then performed in 278 unrelated subjects with idiopathic DCM, prospectively identified at the Mayo Clinic. ...
Els macròfags desenvolupen un paper clau en la formació de la lesió dateroma. Les LDL oxidades (LDLox) indueixen lexpressió de la adipocyte fatty acid-binding protein (FABP4) en els macròfags, fet que constitueix un dels desencadenants de levolució daquestes cèl·lules al fenotip de cèl·lula escumosa. Les LDLox són una font daldehids, que són els productes finals de loxidació dels àcids grassos poliinsaturats. El treball realitzat ha estat dissenyat per provar la hipòtesi de la participació dels aldehids en la inducció de lexpressió de FABP4 en macròfags. A causa del caràcter prooxidant daquestes espècies, sha avaluat si aquesta inducció podria relacionar-se amb el sistema antioxidant cel·lular dirigit pel factor de transcripció Nrf2. El treball experimental es va dur a terme amb monòcits de la línia cel·lular THP-1 que es van diferenciar a macròfags mitjançant èsters de forbol. Els aldehids apolars estudiats van ser el 2,4-decadienal (DDE) i lhexanal. La ...
Preadipocyte factor-1 (Pref-1) is a transmembrane protein highly expressed in preadipocytes. Pref-1 expression is, however, completely abolished in adipocytes. The extracellular domain of Pref-1 undergoes two proteolytic cleavage events that generate 50 and 25 kDa soluble products. To understand the function of Pref-1, we generated transgenic mice that express the full ectodomain corresponding to the large cleavage product of Pref-1 fused to human immunoglobulin-γ constant region. Mice expressing the Pref-1/hFc transgene in adipose tissue, driven by the adipocyte fatty acid-binding protein (aP2, also known as aFABP) promoter, showed a substantial decrease in total fat pad weight. Moreover, adipose tissue from transgenic mice showed reduced expression of adipocyte markers and adipocyte-secreted factors, including leptin and adiponectin, whereas the preadipocyte marker Pref-1 was increased. Pref-1 transgenic mice with a substantial, but not complete, loss of adipose tissue exhibited ...
Preadipocyte factor-1 (Pref-1) is a transmembrane protein highly expressed in preadipocytes. Pref-1 expression is, however, completely abolished in adipocytes. The extracellular domain of Pref-1 undergoes two proteolytic cleavage events that generate 50 and 25 kDa soluble products. To understand the function of Pref-1, we generated transgenic mice that express the full ectodomain corresponding to the large cleavage product of Pref-1 fused to human immunoglobulin-γ constant region. Mice expressing the Pref-1/hFc transgene in adipose tissue, driven by the adipocyte fatty acid-binding protein (aP2, also known as aFABP) promoter, showed a substantial decrease in total fat pad weight. Moreover, adipose tissue from transgenic mice showed reduced expression of adipocyte markers and adipocyte-secreted factors, including leptin and adiponectin, whereas the preadipocyte marker Pref-1 was increased. Pref-1 transgenic mice with a substantial, but not complete, loss of adipose tissue exhibited ...
TY - JOUR. T1 - Fatty acid binding protein 4 is a target of VEGF and a regulator of cell proliferation in endothelial cells. AU - Elmasri, Harun. AU - Karaaslan, Cagatay. AU - Teper, Yaroslav. AU - Ghelfi, Elisa. AU - Weng, Mei Qian. AU - Ince, Tan A.. AU - Kozakewich, Harry. AU - Bischoff, Joyce. AU - Cataltepe, Sule. PY - 2009/11. Y1 - 2009/11. N2 - Fatty acid binding protein 4 (FABP4) plays an important role in maintaining glucose and lipid homeostasis. FABP4 has been primarily regarded as an adipocyte- and macrophage-specific protein, but recent studies suggest that it may be more widely expressed. We found strong FABP4 expression in the endothelial cells (ECs) of capillaries and small veins in several mouse and human tissues, including the heart and kidney. FABP4 was also detected in the ECs of mature human placental vessels and infantile hemangiomas, the most common tumor of infancy and ECs. In most of these cases, FABP4 was detected in both the nucleus and cytoplasm. FABP4 mRNA and ...
Alzheimers disease (AD) and vascular dementia (VaD) are intertwined by mixed dementia (MD) harboring varying degrees of AD pathology in combination with cerebrovascular disease. The aim was to assess whether there is a difference in the cerebrospinal fluid (CSF) profile, of selected proteins, between patients with VaD and MD with subcortical vascular disease (SVD), AD, and healthy controls that could contribute in the separation of the groups. The study included 30 controls, 26 SVD patients (9 VaD and 17 MD) and 30 AD patients. The protein panel included total tau (T-tau), hyperphosphorylated tau 181 (P-tau181), amyloid β 1-42 (Aβ1-42), neurofilament light (NF-L), myelin basic protein (MBP), heart fatty acid binding protein (H-FABP), matrix metalloproteinases (MMP-1, -2, -3, -9, and -10), and tissue inhibitors of metalloproteinases (TIMP-1 and -2). Immunochemical methods were utilized for quantification of the proteins in CSF and data analysis was performed with a multivariate discriminant ...
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Fatty acid binding protein 4 (FABP4), also designated aP2 and Adipocyte-specific fatty acid-binding protein (A-FABP), belongs to the fatty acid-binding protein super family whose members have relative molecular masses of -15, 000, and it is exclusively expressed in differentiated adipocytes. FABP4/A-FABP is a predominant cytosolic protein of mature adipocytes, accounting for -6 % of total cellular proteins.. This protein may be an important regulator of systemic insulin sensitivity and lipid and glucose metabolism. Mice deficient in FABP4/A-FABP are protected from development of hyperinsulinemia, hyperglycemia, and insulin resistance in the context of both dietary and genetic obesity. Adipocytes obtained from FABP4/A-FABP -null mice had markedly reduced efficiency of lipolysis in vivo and in vitro and exhibited a 2- to 3-fold decrease in fatty acid release, suggesting that FABP4/A-FABP mediates efflux of fatty acids in normal physiology. In apolipoprotein E-deficient mice, ablation of the ...
The first step in determining whether a fluorescent dye can be used for antibody labeling consists in collecting data on its physical interaction with the latter. In the present study, the interaction between the 2-(2-hydroxy-5-nitrobenzylidene)-1,3-indanedione (HNBID) dye and the IgG1 monoclonal mouse antibody anti-human heart fatty acid binding protein (anti-hFABP) has been investigated by fluorescence and circular dichroism spectroscopies and complementary structural results were obtained by molecular modeling. We have determined the parameters characterizing this interaction, namely the quenching and binding constants, classes of binding sites, and excited state lifetimes, and we have predicted the localization of HNBID within the Fc region of anti-hFABP. The key glycosidic and amino acid residues in anti-hFABP interacting with HNBID have also been identified. A similar systematic study was undertaken for the well-known fluorescein isothiocyanate fluorophore, for comparison purposes. Our results
2-12 years old children undergoing repair of congenital heart defects were included, except for those with server pulmonary arterial hypertension, chromosomal defects, airway and parenchymal lung disease, immunodeficiency, or blood disorders were excluded. Children were equal randomized to EAPC(electroacupuncture preconditioning)or control group. Electroacupuncture was administered on the bilateral P6 acupoint after anesthesia induction, but prior to surgery in EAPC group. Control patients underwent sham placement of the electrode the arm without stimulation. The duration of cardiopulmonary bypass and aortic cross-clamp time was recorded. Myocardial injury was assessed by cardiac heart-type fatty acid-binding protein (HFAP) and cardiac troponin I (cTnI,0.40 ng/mL). 8-isoprostane , C-reactive protein, cytokines were measured pre- and postoperatively ...
Background and aims Epicardial adipose tissue (EAT) is a source of a number of cytokines which could act in the pathogenesis of coronary artery disease (CAD). The potential relationship between known cardiovascular risk factors, such as smoking, dyslipidaemia or diabetes mellitus and EAT humoral signalling, has not been fully elucidated. Therefore, we designed and conducted a cross-sectional study to determine whether selected cardiovascular risk factors are linked to levels of cytokines in epicardial and subcutaneous adipose tissue (SAT). Methods Samples of SAT and EAT were collected from consecutive patients undergoing scheduled cardiac surgery. Tissue concentrations of tumour necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), adipocyte fatty acid-binding protein, leptin, and adiponectin were determined by ELISA. Results We enrolled 140 patients. TNF-alpha and IL-6 concentrations in EAT and SAT were significantly higher in current smokers (CS) than in never smokers (NS) and former ...
TY - JOUR. T1 - Fatty acid-binding protein-hormone-sensitive lipase interaction. T2 - Fatty acid dependence on binding. AU - Jenkins-Kruchten, Anne E.. AU - Bennaars-Eiden, Assumpta. AU - Ross, James R.. AU - Shen, Wen Jun. AU - Kraemer, Fredric B.. AU - Bernlohr, David A.. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2003/11/28. Y1 - 2003/11/28. N2 - Adipose lipolysis is mediated, in part, via interaction of fatty acid-binding protein (FABP) with hormone-sensitive lipase (HSL). Mice with reduced FABP content in fat (adipocyte FABP null) exhibit diminished fat cell lipolysis, whereas transgenic mice with increased FABP content in fat (epithelial FABP transgenic) exhibit enhanced lipolysis. To examine the relationship between the binding of FABP to HSL and activation of catalytic activity, isothermal titration microcalorimetry as well as kinetic analysis using a variety of FABP isoforms have been employed. In the absence of fatty acids, no FABP-HSL association could be ...
BACKGROUND--There are substantial amounts of cytoplasmic heart-type fatty-acid-binding protein (FABP) (15 kDa) in myocardial tissue. The rapid release of FABP into plasma during ischaemia indicates the possibility of using this protein as a biochemical marker for ischaemic myocardial injury. OBJECTIVE--To study the completeness of the release of FABP from damaged tissue in patients with acute myocardial infarction (AMI) and the suitability of serial plasma FABP concentrations for estimation of myocardial infarct size. METHODS--Immunochemically assayed FABP and enzymatically assayed creatine kinase isoenzyme MB (CK-MB) and alpha-hydroxybutyrate dehydrogenase (HBDH) were determined serially in plasma samples from 49 patients with AMI who had been treated with thrombolytic agents within six hours after the onset of AMI. Previously validated circulatory models and a value of 2.6 h-1 for the fractional clearance rate of FABP from plasma were used to calculate cumulative protein release into plasma. ...
Hop on to get the meaning of FABP1 acronym / slang / Abbreviation. The Medical & Science Acronym / Slang FABP1 means... AcronymsAndSlang. The FABP1 acronym/abbreviation definition. The FABP1 meaning is Fatty Acid Binding Protein 1. The definition of FABP1 by AcronymAndSlang.com
FABP5 (fatty acid binding protein 5 (psoriasis-associated)), Authors: Erin Balcom, Rong-Zong Liu, Stanley Poon, Roseline Godbout. Published in: Atlas Genet Cytogenet Oncol Haematol.
Δ(9)-Tetrahydrocannabinol (THC) and cannabidiol (CBD) occur naturally in marijuana (Cannabis) and may be formulated, individually or in combination in pharmaceuticals such as Marinol or Sativex. Although it is known that these hydrophobic compounds can be transported in blood by albumin or lipoprote …
TY - JOUR. T1 - Differential role of CSF fatty acid binding protein 3, α-synuclein, and Alzheimers disease core biomarkers in Lewy body disorders and Alzheimers dementia. AU - Calabresi, Paolo. AU - Chiasserini, Davide. AU - Biscetti, Leonardo. AU - Eusebi, Paolo. AU - Salvadori, Nicola. AU - Frattini, Giulia. AU - Simoni, Simone. AU - De Roeck, Naomi. AU - Tambasco, Nicola. AU - Stoops, Erik. AU - Vanderstichele, Hugo. AU - Engelborghs, Sebastiaan. AU - Mollenhauer, Brit. AU - Parnetti, Lucilla. PY - 2017. Y1 - 2017. N2 - Background: Neurodegenerative disorders such as Alzheimers disease (AD), Parkinsons disease with dementia (PDD), and dementia with Lewy bodies (DLB) share clinical and molecular features. Cerebrospinal fluid (CSF) biomarkers may help the characterization of these diseases, improving the differential diagnosis. We evaluated the diagnostic performance of five CSF biomarkers across a well-characterized cohort of patients diagnosed with AD, DLB, PDD, and Parkinsons disease ...
Production of purified recombinant human aP2 protein, Recombinant human aP2 protein is produced by standard recombinant DNA technology. In the typical case, aP2 is produced by heterologous expression in E. coli strain BL21(D53) transformed with pET11a vector containing the full length human aP2 cDNA (Baxa, C. A., Sha, R. S., Buelt, M. K., Smith, A. J., Matarese, V., Chinander, L. L., Boundy, K. L., and Bernlohr, D. A. (1989). Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA. Biochemistry 28: 8683-8690 and Xu, Z., Buelt, M. K., Banaszak, L. J., and Bernlohr, D. A. (1991). Expression, purification and crystallization of the adipocyte lipid binding protein. J. Biol. Chem. 266: 14367-14370). Purification of aP2 from E. coli is conducted as described by Xu, yielding essentially homogeneous aP2 protein with molecular weight ˜14600 daltons and free of endogenous fatty acids. The purified aP2 is capable of binding up to one mole of free fatty acid ...
The objective of the present study was to investigate the mobilization of individual fatty acids from human white fat cells. Mammary adipose tissue from eight healthy non-obese women in their normal dietary state was collected, and isolated adipocytes were incubated with lipolytic agents. The mobilization of 34 individual fatty acids was measured by comparing the composition of non-esterified fatty acids (NEFA) with that of the triacylglycerols (TAG) from which they originated through lipolysis. Compared with TAG, NEFA were enriched in some polyunsaturated fatty acids with 18-20 carbon atoms. Conversely, the percentage of very-long-chain (20-22 carbon atoms) saturated and monounsaturated fatty acids was approx. 2 times lower in NEFA than in TAG. The relative mobilization (% in NEFA/% in TAG) of the most readily mobilized fatty acid (C20:5,n-3; 2.25) was more than 6-fold higher than that of the least readily mobilized (C22:1,n-11; 0.37). Relationships were found between the molecular structure of ...
Selection for increasing intramuscular fat content would definitively improve the palatability and juiciness of pig meat as well as the sensorial and organoleptic properties of cured products. However, evidences obtained in human and model organisms suggest that high levels of intramuscular fat might alter muscle lipid and carbohydrate metabolism. We have analysed this issue by determining the transcriptomic profiles of Duroc pigs with divergent phenotypes for 13 fatness traits. The strong aptitude of Duroc pigs to have high levels of intramuscular fat makes them a valuable model to analyse the mechanisms that regulate muscle lipid metabolism, an issue with evident implications in the elucidation of the genetic basis of human metabolic diseases such as obesity and insulin resistance. Muscle gene expression profiles of 68 Duroc pigs belonging to two groups (HIGH and LOW) with extreme phenotypes for lipid deposition and composition traits have been analysed. Microarray and quantitative PCR analysis showed
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Oxygen and nutrient limitation are common features of the tumor microenvironment and are associated with cancer progression and induction of metastasis. The inefficient vascularization of tumor tissue also limits the penetration of other serum-derived factors, such as lipids and lipoproteins, which can be rate limiting for cell proliferation and survival. Here we have investigated the effect of hypoxia and serum deprivation on sterol regulatory element-binding protein (SREBP) activity and the expression of lipid metabolism genes in human glioblastoma multiforme (GBM) cancer cells. We found that SREBP transcriptional activity was induced by serum depletion both in normoxic and hypoxic cells and that activation of SREBP was required to maintain the expression of fatty acid and cholesterol metabolism genes under hypoxic conditions. Moreover, expression of stearoyl-CoA desaturase, the enzyme required for the generation of mono-unsaturated fatty acids, and fatty acid-binding protein 7, a regulator of glioma
Prostate cancer is the most commonly occurring form of non-tobacco related cancers of man in the developed world. Our understanding of the molecular pathology of prostate cancer is currently very limited. At present, clinical therapy focuses on androgen blockage by physical or pharmaceutical castration. Previous work in our molecular pathology laboratory has led to the identification of several genes whose elevated expression may contribute to the malignant progression of the prostate cancer cells. One such gene is that coding for human cutaneous fatty acid binding protein (C-F ABP). The work described in this thesis is aimed to study the specific role of C-F ABP on the tumourigenicity of prostate cancer, to investigate whether the elevated expression of C-FABP is related to androgen sensitivity of the cancer cells, and to explore the therapeutic possibility of inhibiting the development and expansion of prostate cancer through suppressing the expression of C-F ABP gene combined with the ...
Els estudis que shan realitzat per a aquesta tesi doctoral, aporten més informació sobre quin és el paper de les Fatty acid binding protein 4 (FABP4) i Fatty acid bing protein (FABP5) en les malalties metabòliques. Es van estudiar un total de 457 pacients, afectats de diabetis tipus 2 i altres alteracions metabòliques com la síndrome metabòlica, lobesitat i la dislipèmia aterogènica. A tots els pacients, sels va realitzar anamnesi, antropometria i exploració física. A 327, sels va realitzar un estudi vascular de caròtides i dadipositat abdominal. A tots els subjectes sels va realitzar bioquímica completa, incloent perfil lipídic, metabolisme de la glucosa, marcadors dinflamació ia més es van incloure FABP4, FABP5 i PCSK9 circulants (després de 6 setmanes de rentat en pacients amb hipolipemiants). Es van estudiar 6 SNPs del gen FABP4, 2 SNPs del gen FABP5 i 2 SNPs del gen PCSK9. Totes les mostres van ser estudiades amb el mètode Liposcale, RMN-2D. Tots els pacients van ...
Animal studies also are shedding light on the complications of the metabolic syndrome. Two years ago, for example, Drs. MacRae Linton and Sergio Fazio at Vanderbilt and their colleague, Gokan Hotamisligil at Harvard found that they could protect mice from atherosclerosis, the build up of fatty deposits in blood vessels, by eliminating a protein linked to insulin resistance.. The adipocyte fatty-acid-binding protein (aP2) is produced by adipocytes (fat cells), which contribute to insulin resistance, and also by macrophages, inflammatory cells that play a crucial role in atherosclerosis.. The researchers studied apoE-deficient mice, a widely used mouse model of high cholesterol and atherosclerosis. Mice that lacked aP2 were dramatically protected from atherosclerosis, even though they remained insulin resistant, suggesting that the expression of aP2 by macrophages promotes atherosclerosis.. This finding was confirmed in the following way: apoE-deficient mice lacking expression of aP2 only in ...
Fatty acid binding protein 4 is an essential molecule for the development of kidney stones: A new understanding of why obesity and nephrolithiasis go hand in ...
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Yan, S.; Elmes, M. W.; Tong, S.; Hu, K.; Awwa, M.; Teng, Y. H.; Jing, Y. Freitag, M.; Gan, Q.; Clement, T.; Wei, L.; Sweeney, J. M.; Joseph, O. M.; Che, J.; Carbonetti, G. S.; Wang, L.; Bogdan, D. M.; Falcone, J.; Smietalo, N.; Zhou, Y.; Ralph, B. P.; Hsu, Hao-Chi; Li, H.; Rizzo, R. C.; Deutsch, D. G.; Kaczocha, M.; Ojima, I. SAR Studies on Truxillic Acid Mono Esters as a New Class of Antinociceptive Agents Targeting Fatty Acid Binding Proteins, European Journal of Medicinal Chemistry, 2018, 154, 233-252 [http://dx.doi.org/10.1016/j.ejmech.2018.04.050 DOI] PMID: ...
Buy Atp5g2 recombinant protein, ATP synthase lipid-binding protein, mitochondrial (Atp5g2) Recombinant Protein-NP_598240.1 (MBS959310) product datasheet at MyBioSource, Recombinant Proteins
Domains and Structures of Q2JFV4 | Francci3_0452 | UPF0678 fatty acid-binding protein-like protein Francci3_0452 - Also known as Y452_FRACC, Francci3_0452. May play a role in the intracellular transport of hydrophobic ligands. Homodimer.... | canSAR
L-FABP is predominantly found in the liver and is thought to be involved in the regulation of lipid transport (2) and metabolism. At present ALT and Aspartate Aminotransferase are used to both detect the presence of liver disease and monitor its progression however more sensitive markers are required due to the lack of specificity of Aspartate Aminotransferase and the fact plasma levels of ALT increase too late (12). Research has shown L-FABP levels to rise significantly after cell damage; levels are also increased a lot earlier than ALT making it a promising new biomarker. (13 ...
Product Pig ATP synthase lipid binding protein, mitochondrial(ATP5G2) ELISA kit From B-Gene - A competitive ELISA for quantitative measurement of Porcine ATP synthase lipid binding protein, mitochondrial(ATP5G2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUTION*1 vial 12. WASH SOLUTION (100 x)*1 vial 13. BALANCE SOLUTION*1 vial 14. INSTRUCTION*1
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The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
Staphylococcus aureus; strain: COL; locus tag: SACOL0435 (SACOL_RS02190); symbol: ychF; product: GTP-dependent nucleic acid-binding protein EngD
Staphylococcus aureus; strain: Newman; locus tag: NWMN_0355 (NWMN_RS02010); symbol: ychF; product: GTP-dependent nucleic acid-binding protein EngD
Mouse monoclonal liver FABP antibody [2G4] validated for WB, ELISA, IHC, Flow Cyt and tested in Human. Immunogen corresponding to recombinant full length…
Anti-human FABP3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human FABP3 protein.
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Fatty acid-binding proteins (FABPs)[sunting , sunting sumber]. Bagian ini tidak memiliki referensi atau sumber tepercaya ... Protein khas CNS seperti pancortin-2 akan berinteraksi dengan protein modulator aktin, Wiskott-Aldrich syndrome protein ... Myelin basic protein (MBP)[sunting , sunting sumber]. Bagian ini tidak memiliki referensi atau sumber tepercaya sehingga isinya ... Protein tau (TP)[sunting , sunting sumber]. Bagian ini tidak memiliki referensi atau sumber tepercaya sehingga isinya tidak ...
"An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, ... fatty acid binding. • ‏GO:0001948 ربط بروتيني. • lipid binding. • long-chain fatty acid binding. ... Storch J, Thumser AE (2000). "The fatty acid transport function of fatty acid-binding proteins". Biochim. Biophys. Acta. 1486 ( ... "Trinucleotide repeat polymorphism at the human intestinal fatty acid binding protein gene (FABP2)". Nucleic Acids Res. 18 (23 ...
... by Targeting Fatty Acid Amide Hydrolase (FAAH) and the Fatty Acid Binding Proteins (FABPs). Frontiers in Pharmacology. 2016;7: ... These include the heat shock proteins (Hsp70s) and fatty acid binding proteins for anandamide (FABPs).[6][7] FABPs such as ... "Fatty Acid-binding Proteins (FABPs) Are Intracellular Carriers for Δ9-Tetrahydrocannabinol (THC) and Cannabidiol (CBD)". ... "Targeting Fatty Acid Binding Protein (FABP) Anandamide Transporters - A Novel Strategy for Development of Anti-Inflammatory and ...
The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of ... Fatty acid binding protein 7, brain (FABP7; also brain lipid binding protein, BLBP), is a human gene. ... cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty ... "Entrez Gene: FABP7 fatty acid binding protein 7, brain". Anthony TE, Mason HA, Gridley T, Fishell G, Heintz N (May 2005). " ...
Fatty Acid-Binding Protein 1). It is also frequently known as liver-type fatty acid-binding protein (LFABP). FABP1 is primarily ... "Binding of fatty acids and peroxisome proliferators to orthologous fatty acid binding proteins from human, murine, and bovine ... "Fatty acids in cell signalling: modulation by lipid binding proteins". Prostaglandins, Leukotrienes, and Essential Fatty Acids ... Altered expression of the protein has been linked to metabolic conditions including obesity. The fatty acid-binding proteins ( ...
Fatty acid binding protein 9, testis". Stejskal D, Karpisek M, Bronsky J (2008). "Serum adipocyte-fatty acid binding protein ... Fatty acid binding protein 9, testis is a protein that in humans is encoded by the FABP9 gene. GRCh38: Ensembl release 89: ... Oko R, Morales CR (Nov 1994). "A novel testicular protein, with sequence similarities to a family of lipid binding proteins, is ... "Interaction of rat hormone-sensitive lipase with adipocyte lipid-binding protein". Proceedings of the National Academy of ...
Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and ... the liver fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid ... Fatty acid binding protein 6, ileal (gastrotropin), also known as FABP6, is a protein which in humans is encoded by the FABP6 ... 2007). "Fatty acid binding protein 6 is overexpressed in colorectal cancer". Clin. Cancer Res. 12 (17): 5090-5095. doi:10.1158/ ...
Structurally, P2 belongs to the family of cytoplasmic fatty acid-binding proteins (FABPs). GRCh38: Ensembl release 89: ... Myelin P2 protein is a protein that in humans is encoded by the PMP2 gene. Myelin protein P2 is a constituent of peripheral ... Ishaque A, Hofmann T, Eylar EH (1982). "The complete amino acid sequence of the rabbit P2 protein". J. Biol. Chem. 257 (2): 592 ... Suzuki M, Kitamura K, Sakamoto Y, Uyemura K (1983). "The complete amino acid sequence of human P2 protein". J. Neurochem. 39 (6 ...
These include the heat shock proteins (Hsp70s) and fatty acid binding proteins (FABPs). 2-Arachidonoylglycerol is synthesized ... It is an ester formed from the omega-6 fatty acid arachidonic acid and glycerol. It is present at relatively high levels in the ... and phosphatidic acid (PAs) by the action of DAG lipase and the hydrolysis of arachidonic acid-containing lysophosphatidic acid ... 2-AG is hydrolyzed in vitro by monoacylglycerol lipase (MAGL), fatty acid amide hydrolase (FAAH), and the uncharacterized ...
Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and ... Fatty acid-binding protein, epidermal is a protein that in humans is encoded by the FABP5 gene. This gene encodes the fatty ... "Calcium-binding protein S100A7 and epidermal-type fatty acid-binding protein are associated in the cytosol of human ... "Molecular cloning and expression of a novel keratinocyte protein (psoriasis-associated fatty acid-binding protein [PA-FABP]) ...
OSUMI, Noriko (2011). "Polyunsaturated Fatty Acids and Fatty Acid Binding Protein Involvements in Neurogenesis". Oleoscience. ... Osumi, Noriko (2016). "Functional analysis of fatty acid signals in brain development". Reports of Research Assisted by the ... Sakayori, Nobuyuki; Osumi, Noriko (2018). "The role of essential fatty acids in brain development". Journal of Lipid Nutrition ... Sakayori, Nobuyuki; OSUMI, Noriko (January 2011). "The function of fatty acid signals in neurogenesis". Japanese Journal of ...
Fatty acid-binding protein 2 (FABP2) also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in ... "An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, ... Storch J, Thumser AE (2000). "The fatty acid transport function of fatty acid-binding proteins" (PDF). Biochim. Biophys. Acta. ... Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine ...
Londraville RL, Duvall CS (October 2002). "Murine leptin injections increase intracellular fatty acid-binding protein in green ... two JAK2-binding motif boxes, and a STAT-binding domain), while the other four forms have only the intra-cellular region. The ... When looking at the available motif for lepr, the model suggests that it would bind easily to lepa1 and not lepa2 (Fig. 2). ... The importance of the conserved tertiary structure of Lep is most likely explained by requirements for specific LepR-binding ...
"Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine ... The hallucinogenic effects of lysergic acid diethylamide (LSD) were discovered by Albert Hofmann, who was originally working ...
... fatty acids and bilirubin. Vitamin D-binding protein binds to vitamin D and its metabolites, as well as to fatty acids. Not ... It makes up around 50% of human plasma proteins. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, ... "Complete amino acid sequence of human vitamin D-binding protein (group-specific component): evidence of a three-fold internal ... The Vitamin-D binding proteins occupy families 1-3. The other albumins are mixed among each other in families 4-6. ECM1 is in ...
... and fatty acid binding proteins (FABPs). It is found that anandamide prefer cholesterol and ceramide more than other membrane ... Studies of piglets show that dietary levels of arachidonic acid and other essential fatty acids affect the levels of anandamide ... Anandamide is derived from the non-oxidative metabolism of arachidonic acid, an essential omega-6 fatty acid. It is synthesized ... Regulation of Fatty Acid Ethanolamide Biosynthesis by Bile Acids". Structure. 23 (3): 598-604. doi:10.1016/j.str.2014.12.018. ...
"SAR studies on truxillic acid mono esters as a new class of antinociceptive agents targeting fatty acid binding proteins". ... His pioneering work on optically pure fluoroamino acids and their use in medicinally active compounds, such as enkephalin, ... and β-amino acids, oligopeptides, peptidomimetics, taxanes and taxoids. This method has been successfully applied for the ... identification of the paclitaxel binding site and its bioactive conformation in the β-tubulin based on photoaffinity labeling ...
They are proteins covalenently bound to fatty acid chains, often granting them an interface for interacting with biological ... A proteolipid is a protein covalently linked to lipid molecules, which can be fatty acids or sterols. The process of such a ... Depending on the type of fatty acid attached to the protein, a proteolipid can often contain myristoyl, palmitoyl, or prenyl ... "Proteolipids - proteins modified by covalent attachment to lipids - N-myristoylated, S-palmitoylated, prenylated proteins, ...
Other biomarkers that have been shown to be useful include BNP, IL-18, and fatty acid binding protein (FABP). However, there is ...
"Sterol regulatory element-binding proteins: activators of cholesterol and fatty acid biosynthesis". Current Opinion in ... Fatty acid synthesis starts with acetyl-CoA and builds up by the addition of two-carbon units. Fatty acid synthesis occurs in ... Long chain fatty acids are negative allosteric regulators of ACC and so when the cell has sufficient long chain fatty acids, ... Lipogenesis encompasses both fatty acid and triglyceride synthesis, with the latter being the process by which fatty acids are ...
"Structural basis of the binding of fatty acids to peptidoglycan recognition protein, PGRP-S through second binding site". ... "Structural and binding studies of C-terminal half (C-lobe) of lactoferrin protein with COX-2-specific non-steroidal anti- ... "Crystal structures of a type-1 ribosome inactivating protein from Momordica balsamina in the bound and unbound states". ... breast cancer regression proteins and matrix metanosomal proteins as well as their complexes with natural and designed ...
... and stage-specific expression of a fatty acid binding protein-like gene from amphioxus Branchiostoma belcheri" (PDF). Acta ...
SREB proteins are indirectly required for cholesterol biosynthesis and for uptake and fatty acid biosynthesis. These proteins ... Sterol regulatory element-binding proteins (SREBPs) are transcription factors that bind to the sterol regulatory element DNA ... Sterol regulatory element-binding transcription factor 1. X-ray crystallography of Sterol Regulatory Element Binding Protein 1A ... SCAP, in turn, can bind reversibly with another ER-resident membrane protein, INSIG. In the presence of sterols, which bind to ...
"Calcium-binding protein S100A7 and epidermal-type fatty acid-binding protein are associated in the cytosol of human ... Ruse M, Broome AM, Eckert RL (July 2003). "S100A7 (psoriasin) interacts with epidermal fatty acid binding protein and localizes ... This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at ... S100 calcium-binding protein A7 (S100A7), also known as psoriasin, is a protein that in humans is encoded by the S100A7 gene. ...
The corresponding specific proteins are expressed in glandular cells of the mucosa, such as fatty acid binding protein FABP6. ... Lipids (fats) are degraded into fatty acids and glycerol. Pancreatic lipase breaks down triglycerides into free fatty acids and ... Products of digestion (sugars, amino acids, and fatty acids) are absorbed into the bloodstream here. The suspensory muscle of ... The three major classes of nutrients that undergo digestion are proteins, lipids (fats) and carbohydrates: Proteins are ...
"Secretion of fatty acid binding protein aP2 from adipocytes through a nonclassical pathway in response to adipocyte lipase ... "Development of a therapeutic monoclonal antibody that targets secreted fatty acid-binding protein aP2 to treat type 2 diabetes ... through their work in an integrated field on lipid trafficking and signaling with a focus on fatty acid binding proteins, de ... including the double stranded RNA dependent protein kinase PKR and the transmembrane protein STAMP2. The Hotamisligil lab also ...
"Polyunsaturated fatty acids decrease the expression of sterol regulatory element-binding protein-1 in CaCo-2 cells: effect on ... Martin DB, Vagelos PR (June 1962). "The mechanism of tricarboxylic acid cycle regulation of fatty acid synthesis". The Journal ... in tissue distribution indicate that ACC1 maintains regulation of fatty acid synthesis whereas ACC2 mainly regulates fatty acid ... is produced which is a building block for new fatty acids and can inhibit the transfer of the fatty acyl group from acyl CoA to ...
Phosphorylated HSL translocates to the LD surface and associates with Perilipin A and Adipocyte fatty acid-binding protein ( ... Perilipin, also known as lipid droplet-associated protein, Perilipin 1, or PLIN, is a protein that, in humans, is encoded by ... Bickel PE, Tansey JT, Welte MA (June 2009). "PAT proteins, an ancient family of lipid droplet proteins that regulate cellular ... which break triglycerides into glycerol and free fatty acids for use in lipid metabolism. In times of energy deficit, Perilipin ...
The hydrophobic surface of the membrane proteins is bound to the apposed lipid fatty acid chains of the membrane bilayer. For ... Polar headgroups of these lipids bind to the hydrophilic part of the membrane protein(s) at the inner and outer surfaces of ... Thermal denaturation of membrane proteins may destroy the secondary and tertiary structure of membrane proteins, exposing newer ... are a set of lipids or lipidic molecules which preferentially bind or stick to the surface of membrane proteins in biological ...
Weisiger RA (October 2002). "Cytosolic fatty acid binding proteins catalyze two distinct steps in intracellular transport of ... such as when multiple proteins come together to form protein complexes, or when DNA-binding proteins bind to their targets in ... such as fatty acids or sterols, can be transported through the cytosol by specific binding proteins, which shuttle these ... Protein molecules that do not bind to cell membranes or the cytoskeleton are dissolved in the cytosol. The amount of protein in ...
Bile acids bind to some other proteins in addition to their hormone receptors (FXR and TGR5) and their transporters. Among ... Regulation of Fatty Acid Ethanolamide Biosynthesis by Bile Acids". Structure. 23 (3): 598-604. doi:10.1016/j.str.2014.12.018. ... Cholic acid is converted into deoxycholic acid and chenodeoxycholic acid into lithocholic acid. All four of these bile acids ... Primary bile acidsEdit. Bile acid synthesis occurs in liver cells, which synthesize primary bile acids (cholic acid and ...
... specificity is gained by coupling targeted proteins to an "E3 ubiquitin ligase". Each E3 ubiquitin ligase binds to a particular ... amino acid therapy may be helpful for regenerating damaged or atrophied muscle tissue. The branched-chain amino acids or BCAAs ... Brioche T, Pagano AF, Py G, Chopard A (April 2016). "Muscle wasting and aging: Experimental models, fatty infiltrations, and ... The protein balance at time of dormancy is also maintained by lower levels of protein breakdown during the winter time. At ...
... essential for the biosynthesis of fatty acids) in S. pneumoniae.[31] Optochin sensitivity in a culture of Streptococcus ... For a bacterium to bind, take up, and recombine exogenous DNA into its chromosome, it must enter a special physiological state ... Atromentin and leucomelone possess antibacterial activity, inhibiting the enzyme enoyl-acyl carrier protein reductase, ( ... induction of transformation by a desoxyribonucleic acid fraction isolated from pneumococcus type III". J Exp Med. 79 (2): 137- ...
1980). Use of the conjugated polyene fatty-acid parinaric-acid in assaying fatty-acids in serum or plasma. Clinical Chemistry ... Role of beer lipid-binding proteins in preventing lipid destabilization of foam. J. Agric. Food Chem., 2002, 50. vsk, nro 26, s ... Conjugated polyene fatty acids as membrane probes: preliminary characterization. Proc. Natl. Acad. Sci. U.S.A., 1975, 72. vsk, ... Gunstone F.D. (1996). Fatty Acid and Lipid Chemistry. Berlin: Springer Verlag, 10. ISBN 0-8342-1342-7.. ...
"Dietary intake of long-chain polyunsaturated fatty acids during the Paleolithic" (PDF). World Rev Nutr Diet: 12-23. doi:10.1159 ... Antarctica was ice-bound throughout the Pleistocene and the preceding Pliocene. The Andes were covered in the south by the ... Large game animals such as deer were an important source of protein in Middle and Upper Paleolithic diets. ...
These free fatty acids spur increased production of cathelicidin, HBD1, and HBD2, thus leading to further inflammation.[45] ... acnes by oxidizing its proteins through the formation of oxygen free radicals and benzoic acid. These free radicals likely ... C. acnes' ability to bind and activate a class of immune system receptors known as toll-like receptors (TLRs), especially TLR2 ... Salicylic acid[edit]. Salicylic acid is a topically applied beta-hydroxy acid that stops bacteria from reproducing and has ...
Sodium salts of fatty acids are used as soap.[197] Pure sodium metal also has many applications, including use in sodium-vapour ... The balance between potassium and sodium is maintained by ion transporter proteins in the cell membrane.[231] The cell membrane ... Sodium tetraphenylborate can also be classified as an organosodium compound since in the solid state sodium is bound to the ... Indeed, transferring of protons between chemicals is the basis of acid-base chemistry.[10]:43 Also unique is hydrogen's ability ...
... retinoic acid bound to albumin, water soluble β-glucuronides of retinol and retinoic acid, and provitamin A carotenoids.[29] ... it was thought that the sole important retinoid delivery pathway to tissues involved retinol bound to retinol-binding protein ( ... "Fatty liver in hypervitaminosis A: synthesis and release of hepatic triglycerides". The American Journal of Physiology. 234 (5 ... It is likely to exert this effect by binding to specific nuclear receptors (members of the retinoic acid receptor or retinoid X ...
These fatty acids inhibitors have been used as drugs to relieve pain because they can act as the substrate, and bind to the ... The active site is a region on an enzyme which a particular protein or substrate can bind to. The active site will only allow ... is modified to include binding of the inhibitor to the free enzyme:. EI. +. S. ⇌. k. 3. k. −. 3. E. +. S. +. I. ⇌. k. −. 1. k. ... When a competitive inhibitor is bound to an enzyme the K. m. {\displaystyle K_{m}}. increases. This means the binding affinity ...
"DNA-binding proteins and evolution of transcription regulation in the archaea". Nucleic Acids Res. 27 (23): 4658-70. doi: ... Middle, a bacterial or eukaryotic phospholipid: 5, fatty acid chains; 6, ester linkages; 7, D-glycerol moiety; 8, phosphate ... No membrane-bound organelles (questioned[56]) or nucleus. No membrane-bound organelles or nucleus. Membrane-bound organelles ... Deppenmeier, U. (2002). "The unique biochemistry of methanogenesis". Prog Nucleic Acid Res Mol Biol. Progress in Nucleic Acid ...
Water constitutes about 90% of this, with protein, trace minerals, fatty material, vitamins, and glucose contributing the ... because the proteins consist of amino acids; some are hydrophilic (attracted to water) and some are hydrophobic (repelled by ... At its isoelectric pH (6.5), it can bind two cations and assume a red or yellow color. These metal complexes are more heat ... Visual representation of protein denaturation. A globular protein becomes unfolded when exposed to heat. ...
This bacterial protein complex is a machine for folding other proteins, which get trapped within the shell. Fatty acid synthase ... Important information on protein synthesis, ligand binding and RNA interaction can be obtained using this novel technique at ... This huge enzyme complex is responsible for building the long chain fatty acids essential for cellular life. A 0.33 nanometer ... "Direct structural insight into the substrate-shuttling mechanism of yeast fatty acid synthase by electron cryomicroscopy". ...
Sterol Regulatory Element Binding Protein 1 and 2).[10] In the presence of cholesterol, SREBP is bound to two other proteins: ... alongside the nonpolar fatty acid chains of the other lipids. Some research indicates that cholesterol may act as an ... Wolkoff AW, Cohen DE (February 2003). "Bile acid regulation of hepatic physiology: I. Hepatocyte transport of bile acids". Am. ... Cholesterol is oxidized by the liver into a variety of bile acids. These in turn are conjugated with glycine, taurine, ...
... a catalyst of mitochondrial beta-oxidation of long chain fatty acids. The HADHB protein catalyzes the final step of beta- ... The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of ... fatty acid metabolic process. • metabolism. • cardiolipin acyl-chain remodeling. • fatty acid beta-oxidation. ... "Mitochondrial trifunctional protein deficiency: a severe fatty acid oxidation disorder with cardiac and neurologic involvement ...
Wnt-protein binding. • protein binding. • protein kinase binding. • ubiquitin protein ligase binding. • transmembrane signaling ... Bile acid. *Cannabinoid (CB1. *CB2, GPR (18. *55. *119)). *EBI2. *Estrogen. *Free fatty acid (1 ... gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed ... Katoh Y, Katoh M (2007). "Conserved POU-binding site linked to SP1-binding site within FZD5 promoter: Transcriptional ...
There are a number of ALS genes that encode for RNA-binding proteins. The first to be discovered was TDP-43 protein,[35] a ... and weak evidence for omega-three fatty acid intake, exposure to extremely low frequency electromagnetic fields, pesticides, ... Once these mutant RNA-binding proteins are misfolded and aggregated, they may be able to misfold normal protein both within and ... "G93A" means that the 93rd amino acid residue in the SOD1 protein has been changed from glycine to alanine. ...
Smith S (December 1994). "The animal fatty acid synthase: one gene, one polypeptide, seven enzymes". 》FASEB Journal》 8 (15): ... Hunter T (January 1995). "Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling". 》Cell》 ... Yoshikawa S, Caughey WS (May 1990). "Infrared evidence of cyanide binding to iron and copper sites in bovine heart cytochrome c ... "Nucleic Acids Research》 41 (Database issue): D764-72. doi:10.1093/nar/gks1049. PMC 3531171. PMID 23203881.. ...
The plasma protein binding of dronabinol and its metabolites is approximately 97%.. ... catalysed by an enzyme to produce cannabigerolic acid,[35] which is cyclized by the enzyme THC acid synthase to give THCA. Over ... Because they are extremely lipid soluble, cannabinoids accumulate in fatty tissues, reaching peak concentrations in 4-5 days. ... Garrett ER, Hunt CA (July 1974). "Physicochemical properties, solubility, and protein binding of Δ9-tetrahydrocannabinol". J. ...
Diaz-Almeyda E, Thome PE, Hafidi M, Iglesias-Prieto R (2010) Differential stability of photosynthetis membranes and fatty acid ... Iglesias-Prieto R, Govind NS, Trench RK (1993) Isolation and characterization of three membrane-bound chlorophyll-protein ... I. Isoenzyme and soluble protein patterns of axenic cultures of S. microadriaticum. Proc R Soc Lond B 207:405-27 ... Heat stress causes inhibition of de novo synthesis of antenna proteins and photobleaching in cultured Symbiodinium. Proc Nat ...
... molecules can also be bound to the fatty acid palmitate, creating ascorbyl palmitate, or else incorporated into ... The HIFalpha prolyl hydroxylases, termed PHDs/EGLNs (prolyl hydroxylase domain proteins/EGL nine homologues), bind to a ... E304 fatty acid esters of ascorbic acid such as ascorbyl palmitate (approved for use as a food additive in the EU,[88] U.S.[89] ... Ascorbic acid is a weak sugar acid structurally related to glucose. In biological systems, ascorbic acid can be found only at ...
In humans, a set of nine amino acids, two fatty acids, thirteen vitamins, and fifteen minerals are considered essential ... A biochemical assembly that contains both proteins and lipids, bound to the proteins, which allow fats to move through the ... protein. A polypeptide chain of amino acids. It is a body-building nutrient.. protist. psychobiology. Also called behavioral ... A process in which proteins or nucleic acids lose the quaternary, tertiary, and secondary structure which is present in their ...
Protein binding. 55%. Metabolism. Liver (mostly UGT1A4-mediated). Elimination half-life. 29 hours. ... Pheneturide; Fatty acids: Valproate. *Valpromide. *Valproate pivoxil; Carboxamides: Carbamazepine#. *Eslicarbazepine acetate. * ... Lamotrigine binds to melanin-containing tissues such as the iris of the eye. The long-term consequences of this are unknown.[51 ... Lamotrigine binds to the eye and melanin-containing tissues which can accumulate over time and may cause toxicity. Prescribers ...
Valproic acid. Valproic acid added to cells from SMA patients increased SMN2 mRNA and protein levels and that the drug directly ... fatty acid Sodium butyrate M (y) 5, 6, 7 ; H (ny) D (y) 11 M (y) 14; R (y) 15; D (y) 16, 18; H (ny) ... Rouaux C, Loeffler JP, Boutillier AL (September 2004). "Targeting CREB-binding protein (CBP) loss of function as a therapeutic ... Valproic acid. Valproic acid treatment increased mutant Htt H3 and H4 acetylation levels comparable to wild-type Htt in ...
peptide binding. • signal transducer activity. • taste receptor activity. • fatty acid binding. • lipid binding. • G-protein ... linoleic acid and oleic acid), as well as decreased neuronal response to oral fatty acids.[10] ... which contain other G-protein coupled taste receptors), and its absence leads to reduced preference to two types of fatty acid ... G-protein coupled receptor 120 is a protein that in humans is encoded by the GPR120 gene.[5][6] ...
Saliva also contains a glycoprotein called haptocorrin which is a binding protein to vitamin B12.[17] It binds with the vitamin ... amino acids and fatty acids into the bloodstream. The circular folds also slow the passage of food giving more time for ... Lipase digests the triglycerides which are broken down into two fatty acids and a monoglyceride. These are then absorbed by ... and gastric acid activates this to the enzyme pepsin which begins the digestion of proteins. As these two chemicals would ...
... and membrane fatty acid composition". J Gerontol A Biol Sci Med Sci. 61 (8): 781-94. doi:10.1093/gerona/61.8.781. PMID 16912094 ... Commonly consumed food components containing calories are carbohydrates, proteins and fat. In preliminary research, some non- ... Prolonged severe CR lowers total serum and free testosterone while increasing sex hormone binding globulin concentrations in ... brain and heart proteins, and mice placed on CR at 19 months of age show an increases in life span.[44] ...
... via sugar-binding proteins called lectins, which recognize specific carbohydrate moieties.[1] Glycosylation is an important ... Fatty acid metabolism. *Fatty acid degradation (Beta oxidation). *Fatty acid synthesis. Other. *Steroid metabolism ... In addition to their function in protein folding and cellular attachment, the N-linked glycans of a protein can modulate a ... Glycans serve a variety of structural and functional roles in membrane and secreted proteins.[1] The majority of proteins ...
The harmful proteins are those that do not break down due to the strong bonds of the protein. IgE antibodies bind to a receptor ... From two reviews, maternal intake of omega-3, long-chain fatty acids during pregnancy appeared to reduce the risks of medically ... Does the amino acid sequence of the transferred proteins resemble the sequence of known allergenic proteins? Are the ... 2018). "Association of breast milk fatty acids with allergic disease outcomes-A systematic review". Allergy. 73 (2): 295-312. ...
... many lipid-binding proteins are cytosolic and localise to the membrane by binding only the headgroups of lipids. Perhaps the ... a saturated fatty acid bonded to carbon-1, an unsaturated fatty acid bonded to carbon-2, and a phosphate group bonded to carbon ... Although some lipid-binding proteins are able to insert themselves into membranes and could hypothetically recognise the type ... Phosphatidic acids are phospholipids which on hydrolysis give rise to one molecule each of glycerol and phosphoric acid and two ...
FABP5P5 fatty acid binding protein 5 pseudogene 5 [Homo sapiens] FABP5P5 fatty acid binding protein 5 pseudogene 5 [Homo ... fatty acid binding protein 5 pseudogene 5provided by HGNC. Primary source. HGNC:HGNC:31068 Gene type. pseudo. RefSeq status. ... FABP5P5 fatty acid binding protein 5 pseudogene 5 [ Homo sapiens (human) ] Gene ID: 100288964, updated on 12-Oct-2019 ... Model RNAs and proteins are also reported here.. Reference GRCh38.p13 Primary Assembly. Genomic * NC_000005.10 Reference GRCh38 ...
FABP2 stands for Fatty Acid Binding protein 2. FABP2 is defined as Fatty Acid Binding protein 2 frequently. ... www.acronymfinder.com/Fatty-Acid-Binding-protein-2-(FABP2).html. *APA style: FABP2. (n.d.) Acronym Finder. (2019). Retrieved ... www.acronymfinder.com/Fatty-Acid-Binding-protein-2-(FABP2).html. *Chicago style: Acronym Finder. S.v. "FABP2." Retrieved ... www.acronymfinder.com/Fatty-Acid-Binding-protein-2-(FABP2).html,FABP2,/a,. ...
Fatty acid-binding proteins (FABPs) are intracellular proteins that mediate AEA transport to its catabolic enzyme fatty acid ... Fatty Acid Amide Hydrolase (FAAH); Fatty Acid Binding Protein; Lipid Trafficking; Lipid Transport; Δ-tetrahydrocannabinol (THC ... Fatty acid-binding proteins (FABPs) are intracellular carriers for Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) J Biol ... suggesting that phytocannabinoids target cellular proteins involved in endocannabinoid clearance. ...
Urinary L-type fatty acid-binding protein can reflect renal tubulointerstitial injury.. Tanaka T1, Doi K, Maeda-Mamiya R, ... This study aimed to elucidate the role of L-type fatty acid-binding protein (L-FABP) in renal tubulointerstitial injury using a ... Urinary L-Type Fatty Acid-Binding Protein Can Reflect Renal Tubulointerstitial Injury ... Urinary L-Type Fatty Acid-Binding Protein Can Reflect Renal Tubulointerstitial Injury ...
Fatty Acid Binding of a DegV family Protein from Thermus thermophilus. Rehse, P.H., Yokoyama, S.. To be published. ... Fatty Acid Binding of a DegV family Protein from Thermus thermophilus. *DOI: 10.2210/pdb2DT8/pdb ... DegV family protein. A. 280. Thermus thermophilus HB8. Mutation(s): 3 Gene Names: TTHA0951. ... Find proteins for Q5SJQ7 (Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)) ...
The fatty-acid-binding proteins (FABPs) are a family of carrier proteins for fatty acids and other lipophilic substances such ... Fatty+Acid-Binding+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH) ... Weisiger RA (2002). "Cytosolic fatty acid binding proteins catalyze two distinct steps in intracellular transport of their ... "The multigene family of fatty acid-binding proteins (FABPs): function, structure and polymorphism". J. Appl. Genet. 47 (1): 39- ...
Hanhoff T, Lucke C, Spener F: Insights into binding of fatty acids by fatty acid binding proteins. Mol Cell Biochem239 :45 -54, ... Fatty acid-binding proteins (FABPs) are cytosolic fatty acid chaperones that play a critical role in systemic regulation of ... SREBP, sterol regulatory element-binding protein. Fatty acid-binding proteins (FABPs) constitute a family of cytosolic lipid ... Fatty acid binding proteins stabilize leukotriene A4: competition with arachidonic acid but not other lipoxygenase products. J ...
Fatty acid binding protein 9Imported. ,p>Information which has been imported from another database using automatic procedures ... Fatty-acid binding protein (FABP) family.UniRule annotation. Automatic assertion according to rulesi ... tr,G3T3F9,G3T3F9_LOXAF Fatty acid binding protein 9 OS=Loxodonta africana OX=9785 GN=FABP9 PE=3 SV=1 ... View protein in InterPro. IPR012674, Calycin. IPR000463, Fatty_acid-bd. IPR031259, ILBP. IPR000566, Lipocln_cytosolic_FA-bd ...
Among the LBPs, fatty acid binding proteins (FABPs) present pref … ... and storage of a large variety of hydrophobic lipophilic molecules including fatty acids, steroids, and other lipids in the ... are soluble proteins responsible for the uptake, transport, ... Fatty Acid-Binding Proteins / chemistry* * Fatty Acid-Binding ... Among the LBPs, fatty acid binding proteins (FABPs) present preferential binding affinities for long-chain fatty acids. While ...
Fatty acid binding protein-7 (FABP-7) has been previously described in brain and breast development, but never before in the ... Decreased expression of FABP-7 in fetal CCAM compared with normal fetal lung at both the RNA and protein levels suggests FABP-7 ... Validation of differential expression was achieved at the RNA and protein levels. FABP-7 was underexpressed in fetal CCAM ... FABP-7 is a member of a family of cytoplasmic proteins that bind long-chain fatty acids and has a role in fatty acid uptake, ...
The fatty-acid-binding proteins (FABPs) are a family of transport proteins for fatty acids and other lipophilic substances such ... by Targeting Fatty Acid Amide Hydrolase (FAAH) and the Fatty Acid Binding Proteins (FABPs)". Frontiers in Pharmacology. 7: 370 ... Levels of fatty-acid-binding protein have been shown to decline with ageing in the mouse brain, possibly contributing to age- ... Fatty+Acid-Binding+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ...
Fatty acid-binding protein, adipocyte. A. 152. Homo sapiens. Mutation(s): 0 Gene Names: FABP4. ... Crystal Structure of Human Adipocyte Fatty Acid Binding Protein (FABP4) at 1.4 Ang. Resolution. Prasad, G.S., Carney, D., Small ... Crystal Structure of Human Adipocyte Fatty Acid Binding Protein (FABP4) at 1.4 Ang. Resolution. *DOI: 10.2210/pdb3Q6L/pdb ...
... fatty acid-binding protein (FABP)4 (or adipocyte FABP or adipocyte protein 2 [aP2]) serves as a carrier protein for fatty acids ... Plasma Fatty Acid-Binding Protein 4, Nonesterified Fatty Acids, and Incident Diabetes in Older Adults. ... OBJECTIVE To examine the relation of fatty acid-binding protein (FABP)4 and nonesterified fatty acids (NEFAs) to diabetes in ... Plasma Fatty Acid-Binding Protein 4, Nonesterified Fatty Acids, and Incident Diabetes in Older Adults ...
... fatty acid binding protein 5 (psoriasis-associated)), Authors: Erin Balcom, Rong-Zong Liu, Stanley Poon, Roseline Godbout. ... Fatty acid binding protein 5, fatty acids, retinoic acid, peroxisome proliferator-activated receptor, breast cancer, prostate ... Expression of fatty acid binding proteins 3 and 5 genes in rat pancreatic islets and INS-1E cells: regulation by fatty acids ... transporter activity fatty acid binding protein binding extracellular region nucleoplasm cytoplasm cytosol plasma membrane ...
... fatty acid binding protein 5 (psoriasis-associated)), Authors: Erin Balcom, Rong-Zong Liu, Stanley Poon, Roseline Godbout. ... retinoic acid binding fatty acid binding protein binding extracellular region nucleus nucleoplasm cytoplasm cytoplasm cytosol ... retinoic acid binding fatty acid binding protein binding extracellular region nucleus nucleoplasm cytoplasm cytoplasm cytosol ... Fatty acid binding protein 5, fatty acids, retinoic acid, peroxisome proliferator-activated receptor, breast cancer, prostate ...
Compare Fatty Acid Binding Protein ELISA Kits and find the right product on antibodies-online.com. ... Order Fatty Acid Binding Protein ELISA Kits for many Reactivities. Chicken, Cow, Dog and more. ... fatty acid binding protein , fatty acid-binding protein , fatty acid-binding protein, liver , liver fatty acid binding protein ... and Fatty Acid Binding Protein Proteins (3) and many more products for this protein. A total of 41 Fatty Acid Binding Protein ...
Type 2 diabetes Diabetic nephropathy Single nucleotide polymorphism β2-Adrenergic receptor Fatty acid binding protein-2 ... Weiss EP, Brown MD, Shuldiner AR, Hagberg JM (2002) Fatty acid binding protein-2 gene variants and insulin resistance: gene and ... Synergistical action of the β2 adrenoceptor and fatty acid binding protein 2 polymorphisms on the loss of glomerular filtration ... The fatty acid-binding protein-2 A54T polymorphism is associated with renal disease in patients with type 2 diabetes. Diabetes ...
Adipocyte fatty acid binding protein (A-FABP) is a key mediator of obesity-related metabolic syndrome (MetS). The aim of this ... and logarithmically transformed high sensitive C-reactive protein (log-hs-CRP; ,svg xmlns:xlink=http://www.w3.org/1999/xlink ... "The relationship between adipocyte fatty acid binding protein-4, retinol binding protein-4 levels and early diabetic ... Adipocyte fatty acid binding protein (A-FABP) is one of the most abundant proteins in mature adipocytes, which is known for the ...
Plasma Introduction Heart type fatty-acid binding proteins (h-FABPs) are members of cytosolic protein family. The name FABP ... One step Fatty Acid Binding Protein Rapid Test Cassete Specimen : serum / ... originates from their ability to adhere fatty... ...
"Revision of the amino acid sequence of human heart fatty acid-binding protein". Cellular Fatty Acid-binding Proteins. Molecular ... Azzazy HM, Pelsers MM, Christenson RH (Jan 2006). "Unbound free fatty acids and heart-type fatty acid-binding protein: ... "Structural studies on human muscle fatty acid binding protein at 1.4 A resolution: binding interactions with three C18 fatty ... Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is ...
... non-glycosylated polypeptide chain containing 131 amino acids and having a molecular mass of 15.1kDa. ... Fatty acid-binding protein 2, IFABP, I-FABP, FABPI, FABP-2, Fatty acid-binding protein intestinal, FABP2, MGC133132. ... FABP2 binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain ... intracellular metabolism and/or transport of long-chain fatty acids. FABPs are involved in the modulation of cell growth and ...
Liver-type fatty acid-binding protein (l-FABP) is expressed in the proximal tubules, where it plays a key role in fatty acid ... Kamijo A, Kimura K, Sugaya T, Yamanouchi M, Hikawa A, Hirano N, Hirata Y, Goto A, Omata M: Urinary fatty acid-binding protein ... Suzuki K, Babazono T, Murata H, Iwamoto Y: Clinical significance of urinary liver-type fatty acid-binding protein in patients ... Cigarette Smoking Affects Urinary Liver-Type Fatty Acid-Binding Protein Concentration in Patients With Early Diabetic ...
Urinary liver-type fatty acid binding protein (L-FABP) increases along with tubular hypoxia and may be a detector of systemic ... Elevation of urinary liver-type fatty acid binding protein after cardiac catheterization related to cardiovascular events ... Elevation of urinary liver-type fatty acid binding protein after cardiac catheterization related to cardiovascular events. ... Urinary liver-type fatty acid binding protein (L-FABP) increases along with tubular hypoxia and may be a detector of systemic ...
Fatty acid binding protein (FABP) was isolated from chicken liver cytosol. 2. Apparent molecular weight, pI, functional ... 0/Amino Acids; 0/Carrier Proteins; 0/Fabp4 protein, mouse; 0/Fabp7 protein, rat; 0/Fatty Acid-Binding Proteins; 0/Fatty Acids; ... Fatty Acid-Binding Proteins. Fatty Acids / metabolism*. Isoelectric Point. Liver / metabolism*. Molecular Weight. Neoplasm ... Kinetics of fatty acid binding ability of glycated human serum albumin.. 1201519 - Effect of glutamic acid on the fatty acid ...
Here, we analyzed the impact of dietary omega-3 alpha-linolenic fatty acid from linseed oil on skeletal muscle telomere biology ... Recent studies have reported the role of these fatty acids in the aging process, explicitly impacting telomere biology. The ... Our results suggest that omega-3 linolenic acid counteracts the elevation of TRF1 levels, which increase with age and due to ... However, muscles of the linseed-oil-supplemented pigs showed lower levels of the shelterin TRF1 protein compared to the control ...
"Intestinal Fatty Acid-Binding Protein" OR "Enteric fatty acid binding protein" OR "Fatty Acid-Binding Proteins, Intestinal- ... Intestinal fatty acid-binding protein (I-FABP) is a low molecular weight (approximately 15 kDa) cytoplasmic protein [8,9]. In ... Ischemia-modified albumin: crosstalk between fatty acid and cobalt binding. Prostaglandins Leukot Essent Fatty Acids. 2018;135: ... 8] Shi H, Wu B, Wan J, Liu W, Su B. The role of serum intestinal fatty acid binding protein levels and D-lactate levels in the ...
We characterized two novel fatty-acid (FA)-binding TsM proteins (TsMFABP1 and TsMFABP2). Native and recombinant proteins bound ... Their binding was specifically inhibited by oleic acid. TsMFABP1exhibited high affinity toward FA analogs, while TsMFABP2 ... structural diversification and following functional divergence to act as FABP or retinol binding protein, similar to the ... intracellular lipid binding proteins of deuterostomian animals. The canal region specific distribution of lipids, retinol and ...
The intracellular lipid-binding protein (iLBP) termed fatty acid-binding protein 5 [fatty acid-binding protein (FABP5), also ... Direct channeling of retinoic acid between cellular retinoic acid-binding protein II and retinoic acid receptor sensitizes ... including cellular retinol-binding proteins (CRBP I-IV) and cellular retinoic acid (RA)-binding proteins (CRABP-I, II; ref. 19 ... The fatty acid-binding protein FABP5 shuttles ligands from the cytosol to the nuclear receptor PPARβ/δ (encoded for by Pparδ), ...
... Jakobsson, E., Alvite, G., Bergfors, T., Esteves ...
Buy native porcine heart Fatty Acid Binding Protein (FABP) for diagnostic manufacturing and laboratory research - Inquire for ... Fatty Acid Binding Protein(s) (FABP) are a class of cytoplasmic proteins that bind long chain fatty acid and play an important ... Fatty acid Binding Protein (FABP) research: Fatty Acid Binding Protein(s)(FABP) are tissue specific intracellular molecules. ... Home / Antigens / Enzymes / Antigens / Standards / Controls / Proteins / Fatty Acid Binding Protein (fabp) ...
  • Fatty acid-binding proteins (FABPs) are intracellular proteins that mediate AEA transport to its catabolic enzyme fatty acid amide hydrolase (FAAH). (nih.gov)
  • By computational analysis and ligand displacement assays, we show that at least three human FABPs bind THC and CBD and demonstrate that THC and CBD inhibit the cellular uptake and catabolism of AEA by targeting FABPs. (nih.gov)
  • Fatty acid-binding proteins (FABPs) are cytosolic fatty acid chaperones that play a critical role in systemic regulation of lipid and glucose metabolism. (diabetesjournals.org)
  • Fatty acid-binding proteins (FABPs) constitute a family of cytosolic lipid chaperones sharing a conserved three-dimensional structure and exhibiting a distinct, tissue-specific expression pattern ( 1 ). (diabetesjournals.org)
  • Because, in the presence of aP2 deficiency, there is strong molecular compensation by mal1 in adipocytes ( 11 ), we have recently generated mice that express neither of these FABPs and observed a marked protection against diet-induced obesity, insulin resistance, type 2 diabetes, and fatty liver disease in these animals ( 12 , 13 ). (diabetesjournals.org)
  • Among the LBPs, fatty acid binding proteins (FABPs) present preferential binding affinities for long-chain fatty acids. (nih.gov)
  • While most of FABPs in vertebrates and invertebrates present similar β-barrel structures with ligands accommodated in their central cavity, parasitic nematode worms exhibit additional unusual α-helix rich fatty acid- and retinol-binding proteins (FAR). (nih.gov)
  • Herein, we report the comparison of extended molecular dynamics (MD) simulations performed on the ligand-free and palmitic acid-bond states of the Necator americanus FAR-1 (Na-FAR-1) with respect to other classical β-barrel FABPs. (nih.gov)
  • On the contrary, protein-ligand complex in β-barrel FABPs fluctuates around a unique conformation. (nih.gov)
  • The FABPs are intracellular carriers that "solubilize" the endocannabinoid anandamide (AEA), transporting AEA to the breakdown by FAAH, and compounds that bind to FABPs block AEA breakdown, raising its level. (wikipedia.org)
  • The cannabinoids (THC and CBD) are also discovered to bind human FABPs (1, 3, 5, and 7) that function as intracellular carriers, as THC and CBD inhibit the cellular uptake and catabolism of AEA by targeting FABPs. (wikipedia.org)
  • Like the nine other distinct FABPs that have been identified, H-FABP is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. (wikipedia.org)
  • The intracellular fatty acid-binding proteins (FABPs) belongs to a multigene family. (wikipedia.org)
  • FABPs are divided into 3 different types: hepatic, intestinal and cardiac which form 14-15 kDa proteins that take part in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. (prospecbio.com)
  • Fatty acid-binding proteins (FABPs) are extensively expressed in animals, involved in the metabolism of fatty acids. (eurekaselect.com)
  • FABPs share a much conserved secondary structure though they have 20% ~ 70% identities at the amino acid level. (eurekaselect.com)
  • The FABPs constitute a superfamily of highly conserved 14- to 15-kD cytosolic lipid-binding proteins. (ahajournals.org)
  • The crystal structure of Rv0813c reveals a new family of proteins that resemble the fatty acid-binding proteins (FABPs) found in eukaryotes. (epfl.ch)
  • Rv0813c adopts the 10-stranded beta-barrel fold typical of FABPs but lacks the double-helix insert that covers the entry to the binding site in the eukaryotic proteins. (epfl.ch)
  • This residue is part of a conserved Arg-X-Tyr motif much like the triad that binds the carboxylate group of fatty acids in FABPs. (epfl.ch)
  • We examined the level of brain-type (B) and liver-type (L) FABP mRNA and the protein expression profiles of both FABPs in renal cell carcinoma. (biomedcentral.com)
  • Fatty acid-binding proteins (FABPs) are involved in the uptake, the intracellular transport, and the delivery of fatty acids to beta-oxidation. (biomedcentral.com)
  • It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. (biomol.com)
  • FABPs are are inovlved in fatty acid uptake, transport, and metabolism. (creativebiomart.net)
  • FABP7 is expressed in radial glia by the activation of Notch receptors and binds DHA with the highest affinity among all of FABPs. (creativebiomart.net)
  • how ligands could access the internalized binding site(s) of FABPs. (nus.edu.sg)
  • FABPs (fatty acid binding proteins) are a group of cytoplasmic, small mol wt (14-15 kDa) and proteins that has widespread tissue distribution. (4adi.com)
  • Fatty acid-binding proteins (FABPs) play regulatory roles at the nexus of lipid metabolism and signaling. (biomedcentral.com)
  • Fatty acid-binding proteins (FABPs) are a family of fatty acid-binding small proteins essential for lipid trafficking, energy storage and gene regulation. (worktribe.com)
  • Recently, it was shown that fatty acid binding proteins (FABPs) performed intra- and extracellular fatty acid transport. (fapesp.br)
  • This study aimed to elucidate the role of L-type fatty acid-binding protein (L-FABP) in renal tubulointerstitial injury using a mouse adenine-induced renal injury model. (nih.gov)
  • The fatty-acid-binding protein s ( FABP s) are a family of carrier proteins for fatty acids and other lipophilic substances such as eicosanoids and retinoids . (wikidoc.org)
  • In animals lacking the adipocyte/macrophage FABP isoforms aP2 and mal1, there is strong protection against diet-induced obesity, insulin resistance, type 2 diabetes, fatty liver disease, and hypercholesterolemic atherosclerosis. (diabetesjournals.org)
  • These results indicated that both decreased body weight and enhanced muscle AMPK activity in aP2-mal1 −/− mice are potentially leptin dependent but improved systemic insulin sensitivity and protection from liver fatty infiltration are largely unrelated to leptin action and that insulin-sensitizing effects of FABP deficiency are, at least in part, independent of its effects on total-body adiposity. (diabetesjournals.org)
  • Fatty acid binding protein-7 (FABP-7) has been previously described in brain and breast development, but never before in the lung. (nature.com)
  • Decreased expression of FABP-7 in fetal CCAM compared with normal fetal lung at both the RNA and protein levels suggests FABP-7 may have a role in pulmonary development and in the pathogenesis of CCAM. (nature.com)
  • OBJECTIVE To examine the relation of fatty acid-binding protein (FABP)4 and nonesterified fatty acids (NEFAs) to diabetes in older adults. (diabetesjournals.org)
  • As one of these adipokines, fatty acid-binding protein (FABP)4 (or adipocyte FABP or adipocyte protein 2 [aP2]) serves as a carrier protein for fatty acids and other lipophilic substances between extra- and intracellular membranes ( 6 , 7 ). (diabetesjournals.org)
  • The FABP5 gene encodes a member of the fatty acid binding protein family which is also known as epidermal or cutaneous FABP. (atlasgeneticsoncology.org)
  • On www.antibodies-online.com are 13 Fatty Acid Binding Protein (FABP) ELISA Kits from 2 different suppliers available. (antibodies-online.com)
  • The lipid binding process in L-FABP is associated with backbone dynamics. (antibodies-online.com)
  • The goat heart-type fatty acid binding protein (H-FABP (show FABP3 ELISA Kits )) expression vector pEGFP- H-FABP (show FABP3 ELISA Kits ) was injected into the testis of 6 mice randomly by liposome mediated transfection. (antibodies-online.com)
  • The rat intestinal fatty acid binding protein (I-FABP) gene exhibits cell-specific as well as regional differences in its expression within the continuously regenerating small intestinal epithelium. (pnas.org)
  • Adipocyte fatty acid binding protein (A-FABP) is a key mediator of obesity-related metabolic syndrome (MetS). (hindawi.com)
  • Adipocyte fatty acid binding protein (A-FABP) is one of the most abundant proteins in mature adipocytes, which is known for the ability to bind fatty acids and related compounds throughout various cellular compartments, including the peroxisomes, mitochondria, endoplasmic reticulum, lipid droplets, and nucleus [ 3 , 4 ]. (hindawi.com)
  • Heart-type Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. (wikipedia.org)
  • Liver-type fatty acid-binding protein ( l -FABP) is expressed in the proximal tubules, where it plays a key role in fatty acid metabolism. (diabetesjournals.org)
  • Urinary liver-type fatty acid binding protein (L-FABP) increases along with tubular hypoxia and may be a detector of systemic circulation injury. (dovepress.com)
  • 1. Fatty acid binding protein (FABP) was isolated from chicken liver cytosol. (biomedsearch.com)
  • 3. Fatty acids bound to liver FABP affect the electrophoretic nature of FABP. (biomedsearch.com)
  • The purpose of this study was to clarify the value of intestinal fatty acid-binding protein (I-FABP) for the early diagnosis of strangulated intestinal obstruction through a meta-analysis. (degruyter.com)
  • The FABP can be divided into nine types of proteins that are named according to the different organs in which they are found. (degruyter.com)
  • PORCINE HEART FATTY ACID BINDING PROTEIN (FABP) bulk quantities and custom fills/buffers available. (leebio.com)
  • Fatty acid Binding Protein (FABP) research: Fatty Acid Binding Protein(s)(FABP) are tissue specific intracellular molecules. (leebio.com)
  • Fatty Acid Binding Protein(s) (FABP) are a class of cytoplasmic proteins that bind long chain fatty acid and play an important role in the intracellular utilization of fatty acids. (leebio.com)
  • In Acute Mycardial Infarction (AMI) cardiac fatty acid binding protein (FABP) is rapidly released from damaged cardiomyocytes into the circulation due to its small size. (leebio.com)
  • Expression of epidermal-type fatty acid-binding protein (E-FABP) and S100A7 has previously been shown to be elevated in psoriatic skin, a disease characterized by. (acronymattic.com)
  • Heart-type fatty acid binding protein (H-FABP) is a tissue-specific protein which is rapidly released into the circulation when the cardiomyocyte injuries happen. (ahajournals.org)
  • Most importantly, expression of adipose FA binding protein (A-FABP) in macrophages facilitated metabolism of excess sFAs for Cer synthesis. (jimmunol.org)
  • BACKGROUND--There are substantial amounts of cytoplasmic heart-type fatty-acid-binding protein (FABP) (15 kDa) in myocardial tissue. (bmj.com)
  • The rapid release of FABP into plasma during ischaemia indicates the possibility of using this protein as a biochemical marker for ischaemic myocardial injury. (bmj.com)
  • Previously validated circulatory models and a value of 2.6 h-1 for the fractional clearance rate of FABP from plasma were used to calculate cumulative protein release into plasma. (bmj.com)
  • However, infarct size estimated from the cumulative release of the proteins and expressed as gram equivalents of healthy myocardium per litre of plasma yielded a comparable value of 4-6 for both FABP and the two enzymes. (bmj.com)
  • Abstract Epidermal fatty acid-binding protein (E-FABP), previously characterized in human keratinocytes, is a cytoplasmic protein of 15 kD that specifically binds fatty acids (FAs). (ahajournals.org)
  • 1 2 3 Each FABP type displays a characteristic pattern of tissue expression and distinct ligand-binding properties, suggesting specific and complementary functions for each protein. (ahajournals.org)
  • We previously characterized E-FABP in human epidermis and showed that it specifically binds FAs, with high affinity for stearic and linoleic acids. (ahajournals.org)
  • To identify early markers of necrotizing enterocolitis (NEC), we hypothesized that continuous abdominal near-infrared spectroscopy (A-NIRS) measurement of splanchnic tissue oxygen saturation and intermittent plasma intestinal fatty-acid binding protein (pI-FABP) measured every 6 hours can detect NEC prior to onset of clinical symptoms. (nih.gov)
  • Intestinal Fatty Acid Binding Protein (I-FABP) in Plasma.Plasma I-FABP values stratified by NEC severity groups demonstrating a precipitous rise in the f-NEC group shortly after the initiation of feeds and a more gradual rise in the nf-NEC group that paralleled clinical disease progression. (nih.gov)
  • In another study, the serum levels of brain-type fatty acid-binding protein (FABP) and H-FABP in patients with dementia-involving diseases were measured. (thefreedictionary.com)
  • Inductions of FABP in hepatic cytosol by administration of tiadenol and clofibric acid were studied in rats, mice and guinea-pigs. (biomedsearch.com)
  • The increase in [1-14C]oleic acid binding capacity was responsible for the increase in the content of FABP in livers. (biomedsearch.com)
  • There was no essential difference in the inductions of FABP, acyl-CoA hydrolases and peroxisomal beta-oxidation between tiadenol and clofibric acid, despite a marked difference in their chemical structures. (biomedsearch.com)
  • It is concluded that the variability in hepatic pharmacokinetics of unchanged [ 3 H]palmitate and its low-molecular-weight metabolites in perfused rat livers is related to levels of L-FABP and not those of other intrahepatic proteins. (physiology.org)
  • Uptake of fatty acids has been shown to parallel the level of liver fatty acid binding protein (L-FABP) ( 22 ). (physiology.org)
  • Although the variation in the content of various intrahepatic proteins in liver disease has been used to account for the uptake, binding, and metabolism of solutes ( 20 ), the influence of intrahepatic proteins other than L-FABP [e.g., microsomal protein (MP), cytochrome P -450 (CYP), albumin (Alb), and glutathione S -transferase (GST)] on the hepatic disposition of [ 3 H]palmitate has been studied to a limited extent. (physiology.org)
  • To the Editor We read with interest the systematic review and meta-analysis by Bruins Slot et al concerning early diagnosis of myocardial infarction (MI) using heart-type fatty acid-binding protein (H-FABP). (bmj.com)
  • Effects of genetic variation in porcine adipocyte and heart fatty acid-binding protein genes, A-FABP and H-FABP, respectively, on intramuscular fat (IMF) content and backfat thickness (BFT) were examined in F2 crossbreds of Meishan and Western pigs. (wur.nl)
  • Objective- Adipocyte fatty acid-binding protein (A-FABP) abundantly expressed in mature adipocytes and activated macrophages has dramatic effects on atherosclerosis in mice. (ahajournals.org)
  • Proteomic studies have identified the fatty acid binding protein (FABP) as one of molecules present in the parasite ESPs. (asm.org)
  • Most of the residues forming the internal surface of the cavity are conserved in homologous protein sequences found in CG-rich prokaryotes, strongly suggesting that Rv0813c is a member of a new family of bacterial FABP-like proteins that may have roles in the recognition, transport, and/or storage of small molecules in the bacterial cytosol. (epfl.ch)
  • Western-blot analysis using antiserum to 3T3-L1-cell fatty acid binding protein (FABP) revealed that pig adipose tissue contains a 15 kDa protein immunologically similar to the murine protein. (biochemj.org)
  • however, cleavage with CNBr allowed recovery of a 12-amino-acid peptide which was identical with the murine a-FABP sequence (residues 36-48) at 10 of 12 positions. (biochemj.org)
  • The pig a-FABP bound 12-(9-anthroyloxy)oleic acid saturably and stoichiometrically, with an apparent dissociation constant of 1.0 microM. (biochemj.org)
  • Fatty acid-binding proteins (FABP) are involved in the intracellular transport of fatty acids (FA). (biomedcentral.com)
  • Objectives The purpose of this study was to establish the prognostic value of measuring heart fatty acid-binding protein (H-FABP) in patients with suspected acute coronary syndrome (ACS) (in particular, low- to intermediate-risk patients), in addition to troponin measured with the latest third-generation troponin assay. (onlinejacc.org)
  • The fatty acid-binding protein (FABP) family consists of a number of conserved cytoplasmic proteins with roles in intracellular lipid transport, storage, and metabolism. (garvan.org.au)
  • Heart-type fatty acid-binding protein (H-FABP) is a novel marker of myocardial injury and has been reported to be associated with cardiovascular diseases (CVD) including patients with diabetes mellitus (DM). (springer.com)
  • Udenze, Ifeoma 2018-02-01 00:00:00 Background/purposeSerum Intestinal Fatty-Acid Binding Protein (I-FABP) is a useful marker of bowel necrosis in pediatric intussusception. (deepdyve.com)
  • Members of the fatty acid binding protein (FABP) family have been widely reported to act as intracellular lipid binding proteins for hydrophobic lipid species in aqueous cytosolic environments. (nottingham.ac.uk)
  • Members of the fatty acid binding protein (FABP) family have been identified in Echinococcus granulosus, one of which, EgFABP1 is expressed at the tegumental level in the protoscoleces, but it has also been described in both hydatid cyst fluid and secretions of protoscoleces. (gla.ac.uk)
  • WB Control-Rat Heart Fatty acid binding protein (FABP) purified WB +Ve control-Alpha Diagnostic International Inc. (4adi.com)
  • FABP protein were purified (>95%) from the cardiac/hearts of various species. (4adi.com)
  • FABP protein was purified (>95%) from human heart (#FABP11-C) or rat heart (#FABP13-C). For Western blot +ve control (Cat # FABP11-C or FABP13-C) is supplied in SDS-PAGE sample buffer (reduced). (4adi.com)
  • Heart fatty acid-binding protein gene (H-FABP) is an important candidate gene for meat quality. (thefreedictionary.com)
  • Adipocyte fatty acid-binding protein (A-FABP), which belongs to the FABP family, plays an essential role in long-chain fatty acid uptake and metabolic homeostasis, especially in adipose tissue. (koreascience.or.kr)
  • Liver-type fatty-acid binding protein (L-FABP) is expressed in the proximal tubules of the human kidney and participates in fatty acid metabolism. (indianjnephrol.org)
  • FFAs loaded on the proximal tubules are then bound to L-FABP and transported to mitochondria or peroxisomes, where they are metabolized by β-oxidization. (indianjnephrol.org)
  • [3] Human L-FABP gene expression in the kidney was found to be up-regulated and urinary excretion of human L-FABP was increased by stress, such as urinary protein overload, tubular stretch, hypertension, ischemia-reperfusion, and toxins that cause tubulointerstitial damage. (indianjnephrol.org)
  • In particular, the intronless FABP genes, in most of which the key residues involved in fatty acid binding varied, were common in five phyla. (worktribe.com)
  • Heart-type fatty acid binding protein (H-FABP): early marker of acute cardiac syndrome and risk stratification in pulmonary embolism. (advimmuno.com)
  • Our previous studies have demonstrated that expression of epidermal fatty acid binding protein (E-FABP) in tumor associated macrophages (TAMs) promotes macrophage anti-tumor activity by enhancing IFNβ responses in tumor models. (umn.edu)
  • Although EI-05 is unable to bind E-FABP directly, it significantly increases E-FABP expression in macrophages during inflammation. (umn.edu)
  • Multiple molecular dynamics (MD) simulations of fully solvated rat intestinal fatty acid binding protein (I-FABP) were conducted to investigate the dynamics of internal water molecules. (elsevier.com)
  • Exchange of internal water in I-FABP appears to occur almost exclusively through the interface of β-strands EF with the rest of the protein, which has significant implications for the pathways of the fatty acid entry and exit from the binding cavity. (elsevier.com)
  • H-FABP is a small cytoplasmic protein found in the heart and skeletal muscles. (randoxonlinestore.com)
  • The Randox Heart-Type Fatty Acid Binding Protein Assay is an immunoturbidimetric assay and provides quantitative determination of H-FABP in serum with the first result generated in just 14 minutes. (randoxonlinestore.com)
  • Fatty acid-binding protein 4 (FABP4/A-FABP/aP2) is expressed in both adipocytes and macrophages. (ovid.com)
  • GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. (antibodies-online.com)
  • They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. (wikipedia.org)
  • Adipocyte metabolism in adipocyte fatty acid binding protein knockout mice (aP2-/-) after short-term high-fat feeding: functional compensation by the keratinocyte [correction of keritinocyte] fatty acid binding protein. (diabetesjournals.org)
  • To observe the longitudinal changes in serum adipocyte fatty acid-binding protein (AFABP), carbohydrate, and lipid metabolism parameters in women with and without gestational diabetes mellitus (GDM) during mid- and late pregnancy periods, as well as to identify whether there is any association between AFABP and development of GDM. (unboundmedicine.com)
  • The involvement of this protein in cholesterol, bile-acid and triacylglycerol metabolism is discussed. (portlandpress.com)
  • Peroxisomes are important organelles in plant metabolism, containing all the enzymes required for fatty acid β-oxidation. (plantphysiol.org)
  • Although the expression behavior was not related to the survival outcome of the RCC patients, it can be assumed that these changes indicate fundamental alterations in the fatty metabolism in the RCC carcinogenesis. (biomedcentral.com)
  • Loss of the acyl-CoA binding protein (Acbp) results in fatty acid metabolism abnormalities in mouse hair and skin. (semanticscholar.org)
  • Proper fatty acid metabolism is critical for hair and skin development and maintenance. (semanticscholar.org)
  • Fatty acids are important for general cellular metabolism. (4adi.com)
  • Heart fatty acid-binding protein and myoglobin can accurately detect successful reperfusion as early as 15 min after reperfusion [Abstract]. (thefreedictionary.com)
  • abstract = "Much of the recent effort in protein folding has focused on the possibility that residual structures in the unfolded state may provide an initiating site for protein folding. (elsevier.com)
  • Lipid-binding proteins (LBPs) are soluble proteins responsible for the uptake, transport, and storage of a large variety of hydrophobic lipophilic molecules including fatty acids, steroids, and other lipids in the cellular environment. (nih.gov)
  • Most studies investigating the fatty acid uptake into the liver have used isolated hepatocytes with some studies using intact livers ( 26 , 38 ). (physiology.org)
  • The transporter required for fatty acid uptake into peroxisomes has not yet been characterized in any plant species. (plantphysiol.org)
  • It has been hypothesized that the mutation may result in enhanced intestinal uptake of fatty acids, and thereby an impairment of insulin action. (semanticscholar.org)
  • Low Abdominal NIRS Values and Elevated Plasma Intestinal Fatty Acid-Binding Protein in a Premature Piglet Model of Necrotizing Enterocolitis. (nih.gov)
  • D-Lactate and Intestinal Fatty-Acid Binding Protein (IFABP) are markers of intestinal mucosa integrity and barrier function. (ahajournals.org)
  • An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increased fat oxidation, and insulin resistance. (semanticscholar.org)
  • Evidence for linkage of postchallenge insulin levels with intestinal fatty acid-binding protein (FABP2) in Mexican-Americans. (semanticscholar.org)
  • This problem has been simplified for intestinal fatty acid binding protein (IFABP) by incorporating fluorinated aromatic amino acids during synthesis in Escherichia coli. (elsevier.com)
  • Ropson, IJ , Boyer, JA & Dalessio, PM 2006, ' A residual structure in unfolded intestinal fatty acid binding protein consists of amino acids that are neighbors in the native state ', Biochemistry , vol. 45, no. 8, pp. 2608-2617. (elsevier.com)
  • Multiple phases have been observed during the folding and unfolding of intestinal fatty acid binding protein (WT-IFABP) by stopped-flow fluorescence. (elsevier.com)
  • 3Q6L: Crystal Structure of Human Adipocyte Fatty Acid Binding Protein (FABP4) at 1.4 Ang. (rcsb.org)
  • FABP4 is expressed by adipocytes, where it makes up ~8% of the total protein of mature adipocytes, and by macrophages ( 8 , 9 ). (diabetesjournals.org)
  • Adipocyte-fatty acid binding protein (aP2) or FABP4 is expressed predominantly in adipose and macrophages. (ahajournals.org)
  • Although fatty acid binding protein 4 (FABP4) may increase risk of diabetes and exert negative cardiac inotropy, it is unknown whether plasma concentrations of FABP4 are associated with incidence of sudden cardiac death (SCD). (harvard.edu)
  • Here we show that fatty acid binding protein 4 (FABP4/aP2) is an adipokine released from adipocytes under obesogenic conditions, such as hypoxia, to augment insulin secretion. (garvan.org.au)
  • FABP4 is a fatty acid binding protein found in adipocytes. (biomol.com)
  • Synthetic peptide corresponding to amino acid residues surrounding Y20 of human FABP4 (KLH). (biomol.com)
  • Background: Fatty acid-binding protein 4 (FABP4) has been linked to metabolic syndrome development, diabetes, and arteriosclerosis, but the role of FABP4 in target organ damage has not been assessed. (aaccjnls.org)
  • Among them, some members of the lipocalin family-retinol-binding protein 4 (RBP4), 1 lipocalin-2, and fatty acid-binding protein 4 (FABP4)-have been suggested to play important roles in the mechanisms of insulin resistance. (aaccjnls.org)
  • Fatty acid binding protein 4 (FABP4) is an abundant protein in adipocytes, and its production is influenced by high-fat diet (HFD) or obesity. (oncotarget.com)
  • Here, we show that high FABP4 expression and its secretion by PCa cells directly stimulated PCa cell invasiveness by upregulating matrix metalloproteinases through phosphatidylinositol 3-kinase and mitogen-activated protein kinase signaling pathways. (oncotarget.com)
  • MBS700911 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the fatty acid binding protein 4, adipocyte (FABP4) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Standards and samples are pipetted into the wells and any FABP4 present is bound by the immobilized antibody. (mybiosource.com)
  • Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP4 bound in the initial step. (mybiosource.com)
  • Objective: To examine the association between fatty acid binding protein 4 (FABP4) and pre-eclampsia risk in women with type 1 diabetes. (qub.ac.uk)
  • Mice null for adipocyte fatty acid binding protein (AFABP) compensate by increasing expression of keratinocyte fatty acid binding protein (KFABP) (Hotamisligil et al. (diabetesjournals.org)
  • 2011) New aromatic substituted pyrazoles as selective inhibitors of human adipocyte fatty acid-binding protein. (guidetopharmacology.org)
  • 2007) Potent and selective biphenyl azole inhibitors of adipocyte fatty acid binding protein (aFABP). (guidetopharmacology.org)
  • HKU Scholars Hub: Adipocyte fatty acid binding protein as a potential circulating metabolic hormone: clinical and functional studies. (hku.hk)
  • 1998. The adipocyte fatty acid-binding protein locus: Characterization and association with intramuscular fat content in pigs. (koreascience.or.kr)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. (uniprot.org)
  • Since altered sympathetic nerve activity and insulin resistance are implicated in the pathogenesis of type 2 diabetic nephropathy, we investigated the effect of polymorphic Arg16Gly and Gln27Glu in the β2 adrenoceptor gene and Ala54Thr in the fatty acid binding protein 2 gene on the estimated glomerular filtration rate (eGFR) in Chinese patients with the above disease. (springer.com)
  • Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. (wikipedia.org)
  • Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. (wikipedia.org)
  • We have generated mutant mice for epidermal-type fatty acid binding protein by the gene targeting technique and examined the phenotype in detail. (acronymattic.com)
  • A gene on chromosome 6q22-q23 that encodes a brain fatty acid-binding protein possibly involved in the transport of an as-yet unknown hydrophobic ligand with potential morphogenic activity during CNS development. (thefreedictionary.com)
  • Fatty acid synthase (FAS) and acetyl-CoA carboxylase mRNA levels and gene and protein expression of SREBPs were estimated. (biochemj.org)
  • SREBP-2 gene or mature protein expression was not altered. (biochemj.org)
  • In CaCo-2 cells, polyunsaturated fatty acids decrease gene and protein expression of SREBP-1 and FAS mRNA, probably through interference with LXR activity. (biochemj.org)
  • Alanine to threonine substitution at codon 54 of the fatty acid-binding protein 2 (FABP2) gene was recently shown to be associated with insulin resistance in Pima Indians. (semanticscholar.org)
  • This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. (cancer.gov)
  • Duchenne muscular dystrophy is a neuromuscular disorder, which is caused by abnormalities in the DMD gene that encodes the membrane cytoskeletal protein dystrophin. (docwirenews.com)
  • 1992. Fatty acid regulation of gene expression. (koreascience.or.kr)
  • 1999. Effect of genetic variants of the heart fatty acid-binding protein gene on intramuscular fat and performance traits in pigs. (koreascience.or.kr)
  • Although aP2 exhibits higher affinity toward saturated long-chain fatty acids, it also binds to unsaturated fatty acids and retinoic acid ( 3 , 4 ). (diabetesjournals.org)
  • FABP5 can bind retinoic acid as well as polyunsaturated and saturated fatty acids. (atlasgeneticsoncology.org)
  • Transport of FABP5 ligands such as arachidonic acid and retinoic acid to the nucleus is believed to activate the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARβ/δ). (atlasgeneticsoncology.org)
  • The association constants for the complex formation are 1.03 × 106M-1, 1.92 × 105M-1, 2.38 × 105M-1 or 1.25 × 105M-1 for cholesteryl oleate, oleic acid, retinol, or retinoic acid at pH 7.4. (ovid.com)
  • Analysis of the equilibrium binding data from binding assay using [H3]-retinol and [H3]-retinoic acid reveals a singular type of retinoid-binding site in the protein with the association constant of 4.92 × 105M-1 and 1.17 × 105M-1 for retinol and retinoic acid, respectively. (ovid.com)
  • Concomitant increase by peroxisome proliferators of fatty acid-binding protein, peroxisomal beta-oxidation and cytosolic acyl-CoA hydrolase in liver. (biomedsearch.com)
  • Concomitantly an increase of fatty-acid binding by hepatic cytosolic proteins was observed, which could be attributed to an elevation of the content of the fatty-acid-binding protein (Mr 12000). (portlandpress.com)
  • In the current study, we continued the effort to explore the dynamical properties of liver fatty acid binding protein (LFABP) using NMR spectroscopy and MD simulation techniques, aiming at advancing our knowledge on this interesting topic. (nus.edu.sg)
  • Although it seems clear that saturated FAs (sFA [e.g., palmitic acid (PA) and stearic acid (SA)]) are more lipotoxic than unsaturated FAs (uFAs [e.g., oleic acid (OA) and linoleic acid (LA)]) to various types of cells, whether and how they are transported and metabolized in macrophages to induce chronic inflammation in obesity is unclear ( 14 - 16 ). (jimmunol.org)
  • In rats and mice, [1-14C]oleic acid-binding capacity of hepatic cytosol was increased, in association with induction of peroxisomal beta-oxidation, by the administration of either the two peroxisome proliferators. (biomedsearch.com)
  • In an ex vivo experiment using isolated upper small intestine, oleic acid induced only a slight increase in GIP release, which was markedly enhanced by coadministration of bile and oleic acid together with attenuated GIP response in the FABP5 −/− sample. (physiology.org)
  • Fluorescence-based ligand binding analysis confirmed that the recombinant Hp-FAR-1 (rHp-FAR-1) binds the fluorescent fatty acid analog 11-((5-[dimethylaminoaphthalene-1-sulfonyl)amino)undecanoic acid (DAUDA), and by competition oleic acid. (gla.ac.uk)
  • FABP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 131 amino acids and having a molecular mass of 15.1kDa. (prospecbio.com)
  • Previous studies performed with recombinant forms of F. hepatica proteins, such as CatL1, GST, and TxP, have shown that these proteins interact with cells of immune system in animal model of fascioliasis ( 14 - 16 ). (asm.org)
  • We first found that bacterial recombinant EgFABP1 is loaded with fatty acids from the synthesising bacteria, and that fatty acid binding increases its resistance to proteinases, possibly due to subtle conformational changes induced on EgFABP1. (gla.ac.uk)
  • Recombinant Human Fatty Acid Binding Protein-7 RECOMBINANT & NATURAL PROTEINS Human samples 80 % of the research is conducted on human samples. (antibody-antibodies.com)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Recombinant Human Fatty Acid Binding Protein-7 RECOMBINANT & NATURAL PROTEINS. (antibody-antibodies.com)
  • FABP5 (mal1) is detected more broadly, including in epidermal cells, brain, kidney, and lung, but is also expressed in adipocytes and macrophages, where it binds to fatty acids ( 5 ). (diabetesjournals.org)
  • Adipocytes isolated from AFABP-KO and WT mice fed high- or low-fat diets exhibited similar rates of basal and norepinephrine-stimulated lipolysis and insulin-stimulated rates of glucose conversion to fatty acids and glyceride-glycerol. (diabetesjournals.org)
  • However, basal glucose conversion to fatty acids was higher in adipocytes of AFABP-KO mice. (diabetesjournals.org)
  • Our analysis showed that fatty acid binding protein 7 (FABP7) has the highest mean differential overexpression in ccRCC compared to normal kidney. (biomedcentral.com)
  • prolyl hydroxylase 3 (PHD3), fatty acid binding protein 7 (FABP7), carbonic anhydrase IX (CAIX), NADH dehydrogenase 1 alpha subcomplex, 4-like 2 (NDUFA4L2), and monocarboxylate transporter 4 (MCT4). (biomedcentral.com)
  • To the best of our knowledge, this is the first report about the complementary role of fatty acid binding protein 6 (FABP6) for FABP7 in ccRCC. (biomedcentral.com)
  • FABP7 is a brain fatty acid binding protein. (creativebiomart.net)
  • The FABP7 protein solution contains 20mM Tris-HCl pH-8 and 50% Glycerol. (creativebiomart.net)
  • FABP7 although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). (creativebiomart.net)
  • The fatty acid-binding protein FABP5 shuttles ligands from the cytosol to the nuclear receptor PPARβ/δ (encoded for by Pparδ ), thereby enhancing the transcriptional activity of the receptor. (aacrjournals.org)
  • However, it is becoming increasingly clear that these proteins also have specific functions through which they regulate the biologic activities of their ligands (reviewed in ref. 20 ). (aacrjournals.org)
  • Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. (biomol.com)
  • Lipid species include the endocannabinoid anandamide (AEA) and the linoleic acid metabolite 13 (S) hydroxyoctadecadienoic acid (HODE) which act as endogenous TRPV1 ligands (endovanilloids) by evoking TRPV1 mediated Ca2+ entry. (nottingham.ac.uk)
  • Isolation, characterization, and expression of fatty acid binding protein in the liver of Gallus domesticus. (biomedsearch.com)
  • 2009) Identification and characterization of a small molecule inhibitor of Fatty Acid binding proteins. (guidetopharmacology.org)
  • Characterization of a BODIPY-labeled fluorescent fatty acid analogue. (surrey.ac.uk)
  • Ten SNPs have been validated in the coding region of FABP5, 7 missense and 3 synonymous (not affecting the amino acid sequence). (atlasgeneticsoncology.org)
  • FABP5 is a member of the intracellular lipid binding protein family. (atlasgeneticsoncology.org)
  • High expression level of FABP5 is correlated with poor patient survival in some cancers ( 9 , 14 ), and it was shown that the protein displays pro-proliferative and antiapoptotic activities ( 13 , 15-18 ). (aacrjournals.org)
  • A recent study shows that fatty acid-binding protein 5 (FABP5) is expressed in murine K cells and is involved in fat-induced GIP secretion. (physiology.org)
  • Furthermore, subsequent cell free competitive binding assays confirmed that FABP5, 7 and 8 could all physically bind to AEA and 13(S)HODE albeit with variable affinities. (nottingham.ac.uk)
  • However, the molecular mechanisms underlying the response of macrophages to elevated fatty acids (FAs) and their contribution to metabolic inflammation in obesity remain to be fully elucidated. (jimmunol.org)
  • The immunological effect of the purified protein, termed Fh12, was assayed in vitro using monocyte-derived macrophages (MDM) obtained from healthy human donors. (asm.org)
  • Predictions of secondary structure based on the Hp-FAR-1 amino acid sequence indicated that an alpha-helix predominates in Hp-FAR-1, possibly with some coiled-coil conformation, with to beta-structure. (gla.ac.uk)
  • Although they have 20 to 70% amino acid sequence identity, these proteins share a conserved tertiary structure comprised of ten beta sheets and two alpha helixes. (worktribe.com)
  • The clinical features, serum AFABP, other adipocytokines (leptin, adiponectin, retinol-binding protein 4), homeostasis model assessment of insulin resistance, and lipid profiles were measured in the second and third trimesters of pregnancy. (unboundmedicine.com)
  • Hydrophobic compounds such as fatty acids, retinoids, and cholesteryl oleate in the protein solution diminish the protein fluorescence. (ovid.com)
  • Analysis of the fluorescence data suggests that the protein has a binding site for hydrophobic ligand. (ovid.com)
  • Thus, a template of nativelike hydrophobic contacts in the unfolded state may serve as an initiating site for folding this β-sheet protein. (elsevier.com)
  • A slow-diffusion/bound model was found to better describe the hepatic disposition of unchanged [ 3 H]palmitate than other pharmacokinetic models. (physiology.org)
  • Calcium-binding protein S100A7 and epidermal-type fatty acid-binding protein are associated in the cytosol of human. (acronymattic.com)
  • The transient receptor potential vanilloid 1 (TRPV1) channel protein is activated by lipid metabolites synthesized in the cytosol of nociceptors in response to noxious stimulation. (nottingham.ac.uk)
  • Long-chain fatty acids (LCFAs) are a major type of lipid but free LCFAs are insoluble in the cytosol. (biomedcentral.com)
  • Homology to ALD protein and other human and yeast peroxisomal transporters suggests that PXA1 imports coenzyme A esters of fatty acids and IBA into the peroxisome for β-oxidation. (plantphysiol.org)
  • Thr-54 protein is associated with increased fat oxidation and resistance. (prospecbio.com)
  • The Arabidopsis pxa1 mutant, originally isolated because it is resistant to the auxin indole-3-butyric acid (IBA), developmentally arrests when germinated without supplemental sucrose, suggesting defects in fatty acid β-oxidation. (plantphysiol.org)
  • Because IBA is converted to the more abundant auxin, indole-3-acetic acid (IAA), in a mechanism that parallels β-oxidation, the mutant is likely to be IBA resistant because it cannot convert IBA to IAA. (plantphysiol.org)
  • Translocator Protein (TSPO) Affects Mitochondrial Fatty Acid Oxidation in Steroidogenic Cells. (semanticscholar.org)
  • MHC molecules are cell surface proteins, which take active part in host immune reactions and involvement of MHC class in response to almost all antigens and it give effects on specific sites. (omicsonline.org)
  • Fragments identified through this approach tend to be high efficiency binders, in which larger percentage of their atoms are directly involved in binding as compared to larger molecules. (omicsonline.org)
  • Binding ability prediction of peptides to major histocompatibility complex (MHC) class I & II molecules is important in vaccine development from fatty-acid-binding protein of the human blood fluke Schistosoma japonicum. (omicsonline.org)
  • This case study offers an example of how the biophysical properties of proteins could be influenced by buffer molecules, which would deserve the attention of scientists in the in vitro manipulation of protein molecules. (nus.edu.sg)
  • 1994. Fatty acids as signal transducing molecules: involvement in the differentiation of preadipose to adipose cells. (koreascience.or.kr)
  • The computational model employed predicts that w135 (internal) and w217 (located on the protein surface) may be the water molecules with long residence times observed in previously reported magnetic relaxation dispersion studies. (elsevier.com)
  • The average residence time of ∼20 internal water molecules occupying the fatty acid binding cavity is estimated to be between 0.6 and 2.0 nanoseconds. (elsevier.com)
  • FABP2 binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. (prospecbio.com)
  • The results suggest that this protein may be actively involved in the exchange and transport of fatty acids between different membranes and cellular compartments within the parasite. (gla.ac.uk)
  • The environments around Trp-31 and Trp-81 of this protein were studied by intrinsic fluorescence and solute quenching. (ovid.com)
  • The unfolding and refolding kinetics of the mutant protein containing only tryptophan 82 (W6Y-IFABP) showed that the tryptophan at this location was critical to the fluorescence signal changes observed throughout the unfolding reaction and early in the refolding reaction. (elsevier.com)
  • However, the kinetic patterns of the mutant protein containing only tryptophan 6 (W82Y-IFABP) indicated that the tryptophan at this location participated in the fluorescence signal changes observed early in the unfolding reaction and late in the refolding reaction. (elsevier.com)
  • FABP3 is known to interact with TNNI3K in the context of interacting with cardiac troponin I. The protein also interacts with, VPS28, KIAA159, NUP62, PLK1, UBC, and Xpo1. (wikipedia.org)
  • In HIV, a synthetic peptide corresponding to the immunosuppressive domain (amino acids 574-592) of HIV-1 gp41 downregulates the expression of fatty acid binding protein 3 (FABP3) in peptide-treated PBMCs. (wikipedia.org)
  • Further, we show that differential methylation of FABP3 affects binding activity with nuclear proteins from heart tissue. (biomedcentral.com)
  • We postulated that FABP3 might be responsible for fatty acid transport from cumulus cells to the oocytes via transzonal projections (TZPs) during IVM. (fapesp.br)
  • Transcript and protein levels of FABP3 were analyzed in both in vivo- and in vitro-matured cumulus-oocyte-complexes and were increased in IVM samples. (fapesp.br)
  • We therefore speculated that altered traffic of fatty acids via FABP3 during IVM was the mechanism leading to the excess of lipids accumulated within IVM oocytes. (fapesp.br)
  • Furthermore, we demonstrated an increase in FABP3 levels and lipid content during the first 9 h of IVM, further strengthening the possibility of fatty acid transport via FABP3 and TZPs. (fapesp.br)
  • The α-helix fold encompasses a complex internal ligand-binding cavity with a remarkable conformational plasticity that allows reversible switching between distinct states in the holo-Na-FAR-1. (nih.gov)
  • The cavity can change up to one-third of its size affected by conformational changes of the protein-ligand complex. (nih.gov)
  • These changes in the ligand conformation follow changes in the cavity size dictated by the transient protein conformation. (nih.gov)
  • The barrel encloses a deep cavity, at the bottom of which a small cyclic ligand was found to bind to the hydroxyl group of Tyr192. (epfl.ch)
  • Trp-31 or/and Trp-81 is in or very near the binding site and the gross conformation of protein changes considerably as the formation of protein-ligand complex. (ovid.com)
  • The experimental result demonstrates that the intrinsic millisecond dynamics of LFABP, somewhat disappointedly, does not represent a critical conformational reorganization required for ligand entry due to the contradiction of timescales, but implies that it may represent a dynamical equilibrium between the apo-state and a state resembling the singly-bound conformation. (nus.edu.sg)
  • Despite fast advancement of experimental techniques for exploring protein dynamics, direct visualization of ligand entry/exit processes which potentially involves multiple transient steps is still formidable nowadays. (nus.edu.sg)
  • Different ligand egress routes were identified for LFABP in this work, which furthered our understanding on the protein-ligand interplay. (nus.edu.sg)
  • At pH 7.4, the spectrum in the UV region appears as one negative band with a minimum mean residue ellipticity of -3,600 deg·cm2·dmole-1 at 217 nm, suggesting a very low or no helical content, but a considerable amount of β-form, β-turn, and unordered form in the protein molecule. (ovid.com)
  • 1972. Influence of muscle location on fatty acid composition of total intramuscular lipids in the pig. (koreascience.or.kr)
  • Evaluation of the diagnostic performance of heart-type fatty acid binding protein in the BWH-TIMI ED chest pain study. (acronymattic.com)
  • Heart-type fatty acid binding protein in early post-cardiac arrest phase is associated with survival to discharge in OHCA patients. (ahajournals.org)
  • Site-directed mutagenesis has been used to examine the role of each of the two tryptophans of this protein in these processes. (elsevier.com)
  • A population association study of four candidate genes (hexokinase II, glucagon-like peptide-1 receptor, fatty acid binding protein-2, and apolipoprotein C-II) with type 2 diabetes and impaired glucose tolerance in Japanese subjects. (semanticscholar.org)
  • Recent reports suggest that CBD and THC elevate the levels of the endocannabinoid anandamide (AEA) when administered to humans, suggesting that phytocannabinoids target cellular proteins involved in endocannabinoid clearance. (nih.gov)
  • nonesterified, long-chain fatty acids (hereafter referred to as fatty acids) play a vital role in many cellular processes. (physiology.org)
  • Some fatty acids are precursors for biological mediators, whereas others may interact with the cell membrane to regulate various cellular functions. (physiology.org)
  • As for AFABP, the rate of fatty acid transfer from KFABP to model phospholipid vesicles was increased with acceptor membrane concentration and by inclusion of acidic phospholipids, indicating a similar mechanism of transfer. (diabetesjournals.org)
  • Search, Find and Buy Antibodies, ELISA Kits and Proteins. (antibodies-online.com)
  • Additionally we are shipping Fatty Acid Binding Protein Antibodies (24) and Fatty Acid Binding Protein Proteins (3) and many more products for this protein. (antibodies-online.com)
  • The DNA encoding Hp-FAR-1 was recovered by screening a fourth larval (L-4) H. polygyrus DNA expression library, using antibodies raised against L-4 stage excretory/secretory (E/S) proteins. (gla.ac.uk)
  • Validation of differential expression was achieved at the RNA and protein levels. (nature.com)
  • Results from the assay indicate that Fh12 produced a significantly increased arginase expression and activity and induced the expression of chitinase-3-like protein (CHI3L1). (asm.org)
  • Although only 18:2, 20:4, 20:5 and 22:6 suppressed SREBP-1, acetyl-CoA carboxylase and FAS expression, all fatty acids decreased the rate of fatty acid synthesis. (biochemj.org)
  • Since all fatty acids decreased fatty acid synthesis, mechanisms other than changes in SREBP-1c expression must be entertained. (biochemj.org)
  • 2001. Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals. (koreascience.or.kr)
  • PXA1 encodes a predicted peroxisomal ATP-binding cassette transporter that is 42% identical to the human adrenoleukodystrophy (ALD) protein, which is defective in patients with the demyelinating disorder X-linked ALD. (plantphysiol.org)
  • The conformation of 24p3 protein purified from mouse uterine luminal fluid was studied by circular dichroism spectroscopy in 200-300 nm. (ovid.com)
  • No increases in urinary protein, albumin, or N-acetyl-beta-D-glucosaminidase levels were found for 6 weeks in any group. (nih.gov)
  • Plasma-free FAs (also called nonesterified FAs) are usually bound to plasma albumin, which usually ranges from 200 to 400 μM in healthy people, but from 400 to 800 μM in obese individuals and diabetic patients ( 12 , 13 ). (jimmunol.org)
  • Free fatty acids (FFAs) are bound to albumin, filtered through the glomeruli, and reabsorbed into the proximal tubules. (indianjnephrol.org)
  • As assessed by Western blot analysis, the level of KFABP protein in AFABP-KOs was approximately 40% of the level of AFABP in WT controls. (diabetesjournals.org)
  • The binding affinities of KFABP for long-chain fatty acids were 2- to 4-fold higher than those of AFABP, but the relative affinities for different fatty acids were similar. (diabetesjournals.org)
  • T0901317 increased endogenous fatty acid synthesis yet did not increase secretion of triacylglycerol-rich lipoproteins. (biochemj.org)
  • Increased endogenous fatty acid synthesis does not promote triacylglycerol-rich lipoprotein secretion. (biochemj.org)
  • [1] These proteins are thought to facilitate the transfer of fatty acids between extra- and intracellular membranes . (wikidoc.org)
  • The shelterin protein complex, located at the extremities of chromosomes, ensures telomere protection and length regulation. (mdpi.com)
  • Regulation of sterol regulatory element-binding proteins (SREBPs) by fatty acid flux was investigated in CaCo-2 cells. (biochemj.org)
  • Likić, VA & Prendergast, FG 2001, ' Dynamics of internal water in fatty acid binding protein: Computer simulations and comparison with experiments ', Proteins: Structure, Function and Genetics , vol. 43, no. 1, pp. 65-72. (elsevier.com)