A GATA transcription factor that is expressed in the MYOCARDIUM of developing heart and has been implicated in the differentiation of CARDIAC MYOCYTES. GATA4 is activated by PHOSPHORYLATION and regulates transcription of cardiac-specific genes.
A GATA transcription factor that is expressed predominately in SMOOTH MUSCLE CELLS and regulates vascular smooth muscle CELL DIFFERENTIATION.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A GATA transcription factor that is found predominately in LYMPHOID CELL precursors and has been implicated in the CELL DIFFERENTIATION of HELPER T-CELLS. Haploinsufficiency of GATA3 is associated with HYPOPARATHYROIDISM; SENSORINEURAL HEARING LOSS; and renal anomalies syndrome.
An essential GATA transcription factor that is expressed primarily in HEMATOPOIETIC STEM CELLS.
A GATA transcription factor that is specifically expressed in hematopoietic lineages and plays an important role in the CELL DIFFERENTIATION of ERYTHROID CELLS and MEGAKARYOCYTES.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A GATA transcription factor that is expressed predominately in SMOOTH MUSCLE CELLS and is involved in the CELL DIFFERENTIATION of CARDIAC MYOCYTES. In the developing heart, GATA5 becomes restricted to the ENDOCARDIUM and regulates transcription of genes such as cardiac TROPONIN C.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.
The series of cells in the red blood cell lineage at various stages of differentiation.
Proteins encoded by homeobox genes (GENES, HOMEOBOX) that exhibit structural similarity to certain prokaryotic and eukaryotic DNA-binding proteins. Homeodomain proteins are involved in the control of gene expression during morphogenesis and development (GENE EXPRESSION REGULATION, DEVELOPMENTAL).
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A group of transcription factors that were originally described as being specific to ERYTHROID CELLS.
A family of transcription factors that contain two ZINC FINGER MOTIFS and bind to the DNA sequence (A/T)GATA(A/G).
A family of DNA-binding transcription factors that contain a basic HELIX-LOOP-HELIX MOTIF.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A multiprotein complex composed of the products of c-jun and c-fos proto-oncogenes. These proteins must dimerize in order to bind to the AP-1 recognition site, also known as the TPA-responsive element (TRE). AP-1 controls both basal and inducible transcription of several genes.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Established cell cultures that have the potential to propagate indefinitely.
A subclass of winged helix DNA-binding proteins that share homology with their founding member fork head protein, Drosophila.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A large superfamily of transcription factors that contain a region rich in BASIC AMINO ACID residues followed by a LEUCINE ZIPPER domain.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A family of zinc finger transcription factors that share homology with Kruppel protein, Drosophila. They contain a highly conserved seven amino acid spacer sequence in between their ZINC FINGER MOTIFS.
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
The so-called general transcription factors that bind to RNA POLYMERASE II and that are required to initiate transcription. They include TFIIA; TFIIB; TFIID; TFIIE; TFIIF; TFIIH; TFII-I; and TFIIJ. In vivo they apparently bind in an ordered multi-step process and/or may form a large preinitiation complex called RNA polymerase II holoenzyme.
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
A ubiquitously expressed zinc finger-containing protein that acts both as a repressor and activator of transcription. It interacts with key regulatory proteins such as TATA-BINDING PROTEIN; TFIIB; and ADENOVIRUS E1A PROTEINS.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.
A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.
The major sequence-specific DNA-binding component involved in the activation of transcription of RNA POLYMERASE II. It was originally described as a complex of TATA-BOX BINDING PROTEIN and TATA-BINDING PROTEIN ASSOCIATED FACTORS. It is now know that TATA BOX BINDING PROTEIN-LIKE PROTEINS may take the place of TATA-box binding protein in the complex.
A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.
An activating transcription factor that plays a key role in cellular responses to GENOTOXIC STRESS and OXIDATIVE STRESS.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The first nucleotide of a transcribed DNA sequence where RNA polymerase (DNA-DIRECTED RNA POLYMERASE) begins synthesizing the RNA transcript.
A specificity protein transcription factor that regulates expression of a variety of genes including VASCULAR ENDOTHELIAL GROWTH FACTOR and CYCLIN-DEPENDENT KINASE INHIBITOR P27.
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
A family of transcription factors that control EMBRYONIC DEVELOPMENT within a variety of cell lineages. They are characterized by a highly conserved paired DNA-binding domain that was first identified in DROSOPHILA segmentation genes.
An activating transcription factor that regulates expression of a variety of GENES including C-JUN GENES; CYCLIN A; CYCLIN D1; and ACTIVATING TRANSCRIPTION FACTOR 3.
An RNA POLYMERASE II specific transcription factor. It plays a role in assembly of the pol II transcriptional preinitiation complex and has been implicated as a target of gene-specific transcriptional activators.
Nucleic acid sequences involved in regulating the expression of genes.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).
An E2F transcription factor that interacts directly with RETINOBLASTOMA PROTEIN and CYCLIN A and activates GENETIC TRANSCRIPTION required for CELL CYCLE entry and DNA synthesis. E2F1 is involved in DNA REPAIR and APOPTOSIS.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A cell line derived from cultured tumor cells.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Activating transcription factors of the MADS family which bind a specific sequence element (MEF2 element) in many muscle-specific genes and are involved in skeletal and cardiac myogenesis, neuronal differentiation and survival/apoptosis.
A family of transcription factors that contain regions rich in basic residues, LEUCINE ZIPPER domains, and HELIX-LOOP-HELIX MOTIFS.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
A family of DNA-binding proteins that are primarily expressed in T-LYMPHOCYTES. They interact with BETA CATENIN and serve as transcriptional activators and repressors in a variety of developmental processes.
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
A basic helix-loop-helix leucine zipper transcription factor that regulates the CELL DIFFERENTIATION and development of a variety of cell types including MELANOCYTES; OSTEOCLASTS; and RETINAL PIGMENT EPITHELIUM. Mutations in MITF protein have been associated with OSTEOPETROSIS and WAARDENBURG SYNDROME.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A signal transducer and activator of transcription that mediates cellular responses to INTERFERONS. Stat1 interacts with P53 TUMOR SUPPRESSOR PROTEIN and regulates expression of GENES involved in growth control and APOPTOSIS.
Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
A subunit of NF-kappa B that is primarily responsible for its transactivation function. It contains a C-terminal transactivation domain and an N-terminal domain with homology to PROTO-ONCOGENE PROTEINS C-REL.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A family of basic helix-loop-helix transcription factors that control expression of a variety of GENES involved in CELL CYCLE regulation. E2F transcription factors typically form heterodimeric complexes with TRANSCRIPTION FACTOR DP1 or transcription factor DP2, and they have N-terminal DNA binding and dimerization domains. E2F transcription factors can act as mediators of transcriptional repression or transcriptional activation.
Recurring supersecondary structures characterized by 20 amino acids folding into two alpha helices connected by a non-helical "loop" segment. They are found in many sequence-specific DNA-BINDING PROTEINS and in CALCIUM-BINDING PROTEINS.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
An activating transcription factor that regulates the expression of a variety of GENES involved in amino acid metabolism and transport. It also interacts with HTLV-I transactivator protein.
A transcription factor that takes part in WNT signaling pathway where it may play a role in the differentiation of KERATINOCYTES. The transcriptional activity of this protein is regulated via its interaction with BETA CATENIN.
An activating transcription factor that regulates expression of a variety of genes including C-JUN GENES and TRANSFORMING GROWTH FACTOR BETA2.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
One of several general transcription factors that are specific for RNA POLYMERASE III. It is a zinc finger (ZINC FINGERS) protein and is required for transcription of 5S ribosomal genes.
A conserved A-T rich sequence which is contained in promoters for RNA polymerase II. The segment is seven base pairs long and the nucleotides most commonly found are TATAAAA.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
The hollow, muscular organ that maintains the circulation of the blood.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Transcription factors that were originally identified as site-specific DNA-binding proteins essential for DNA REPLICATION by ADENOVIRUSES. They play important roles in MAMMARY GLAND function and development.
Cellular DNA-binding proteins encoded by the c-jun genes (GENES, JUN). They are involved in growth-related transcriptional control. There appear to be three distinct functions: dimerization (with c-fos), DNA-binding, and transcriptional activation. Oncogenic transformation can take place by constitutive expression of c-jun.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
A family of transcription factors that share a unique DNA-binding domain. The name derives from viral oncogene-derived protein oncogene protein v-ets of the AVIAN ERYTHROBLASTOSIS VIRUS.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
A SOXE transcription factor that plays a critical role in regulating CHONDROGENESIS; OSTEOGENESIS; and male sex determination. Loss of function of the SOX9 transcription factor due to genetic mutations is a cause of CAMPOMELIC DYSPLASIA.
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
A general transcription factor that is involved in basal GENETIC TRANSCRIPTION and NUCLEOTIDE EXCISION REPAIR. It consists of nine subunits including ATP-DEPENDENT DNA HELICASES; CYCLIN H; and XERODERMA PIGMENTOSUM GROUP D PROTEIN.
Proteins containing a region of conserved sequence, about 200 amino acids long, which encodes a particular sequence specific DNA binding domain (the T-box domain). These proteins are transcription factors that control developmental pathways. The prototype of this family is the mouse Brachyury (or T) gene product.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
An RNA POLYMERASE II specific transcription factor. It may play a role in transcriptional activation of gene expression by interacting with the TATA-BOX BINDING PROTEIN component of TRANSCRIPTION FACTOR TFIID.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A signal transducer and activator of transcription that mediates cellular responses to a variety of CYTOKINES. Stat5 activation is associated with transcription of CELL CYCLE regulators such as CYCLIN KINASE INHIBITOR P21 and anti-apoptotic genes such as BCL-2 GENES. Stat5 is constitutively activated in many patients with acute MYELOID LEUKEMIA.
A transcription factor that possesses DNA-binding and E2F-binding domains but lacks a transcriptional activation domain. It is a binding partner for E2F TRANSCRIPTION FACTORS and enhances the DNA binding and transactivation function of the DP-E2F complex.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Proteins found in any species of fungus.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Nucleotide sequences of a gene that are involved in the regulation of GENETIC TRANSCRIPTION.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.
A ubiquitously expressed octamer transcription factor that regulates GENETIC TRANSCRIPTION of SMALL NUCLEAR RNA; IMMUNOGLOBULIN GENES; and HISTONE H2B genes.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Proteins prepared by recombinant DNA technology.
A general transcription factor that plays a major role in the activation of eukaryotic genes transcribed by RNA POLYMERASES. It binds specifically to the TATA BOX promoter element, which lies close to the position of transcription initiation in RNA transcribed by RNA POLYMERASE II. Although considered a principal component of TRANSCRIPTION FACTOR TFIID it also takes part in general transcription factor complexes involved in RNA POLYMERASE I and RNA POLYMERASE III transcription.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Factors that bind to RNA POLYMERASE III and aid in transcription. They include the assembly factors TFIIIA and TFIIIC and the initiation factor TFIIIB. All combine to form a preinitiation complex at the promotor that directs the binding of RNA POLYMERASE III.
Interacting DNA-encoded regulatory subsystems in the GENOME that coordinate input from activator and repressor TRANSCRIPTION FACTORS during development, cell differentiation, or in response to environmental cues. The networks function to ultimately specify expression of particular sets of GENES for specific conditions, times, or locations.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
A heterotetrameric transcription factor composed of two distinct proteins. Its name refers to the fact it binds to DNA sequences rich in GUANINE and ADENINE. GA-binding protein integrates a variety of SIGNAL TRANSDUCTION PATHWAYS and regulates expression of GENES involved in CELL CYCLE control, PROTEIN BIOSYNTHESIS, and cellular METABOLISM.
Deletion of sequences of nucleic acids from the genetic material of an individual.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
A family of low-molecular weight, non-histone proteins found in chromatin.
An early growth response transcription factor that has been implicated in regulation of CELL PROLIFERATION and APOPTOSIS.
An ets proto-oncogene expressed primarily in adult LYMPHOID TISSUE; BRAIN; and VASCULAR ENDOTHELIAL CELLS.
Proteins found in any species of bacterium.
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. This catalyzes endonucleolytic cleavage of DNA yielding 5'-phosphodi- and oligonucleotide end-products. The enzyme has a preference for double-stranded DNA.
A transcription factor that takes part in WNT signaling pathway. The activity of the protein is regulated via its interaction with BETA CATENIN. Transcription factor 7-like 2 protein plays an important role in the embryogenesis of the PANCREAS and ISLET CELLS.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
A tissue-specific subunit of NF-E2 transcription factor that interacts with small MAF PROTEINS to regulate gene expression. P45 NF-E2 protein is expressed primarily in MEGAKARYOCYTES; ERYTHROID CELLS; and MAST CELLS.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The biosynthesis of DNA carried out on a template of RNA.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Transport proteins that carry specific substances in the blood or across cell membranes.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
A basic helix-loop-helix transcription factor that was originally identified in DROSOPHILA as essential for proper gastrulation and MESODERM formation. It plays an important role in EMBRYONIC DEVELOPMENT and CELL DIFFERENTIATION of MUSCLE CELLS, and is found in a wide variety of organisms.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Cellular DNA-binding proteins encoded by the c-fos genes (GENES, FOS). They are involved in growth-related transcriptional control. c-fos combines with c-jun (PROTO-ONCOGENE PROTEINS C-JUN) to form a c-fos/c-jun heterodimer (TRANSCRIPTION FACTOR AP-1) that binds to the TRE (TPA-responsive element) in promoters of certain genes.
Gated transport mechanisms by which proteins or RNA are moved across the NUCLEAR MEMBRANE.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Formation of an acetyl derivative. (Stedman, 25th ed)
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
One of several general transcription factors that are specific for RNA POLYMERASE III. TFIIIB recruits and positions pol III over the initiation site and remains stably bound to the DNA through multiple rounds of re-initiation by RNA POLYMERASE III.
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
One of the BASIC-LEUCINE ZIPPER TRANSCRIPTION FACTORS that is synthesized as a membrane-bound protein in the ENDOPLASMIC RETICULUM. In response to endoplasmic reticulum stress it translocates to the GOLGI APPARATUS. It is activated by PROTEASES and then moves to the CELL NUCLEUS to regulate GENETIC TRANSCRIPTION of GENES involved in the unfolded protein response.
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
A family of mammalian POU domain factors that are expressed predominately in NEURONS.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
A basic-leucine zipper transcription factor that regulates GLOBIN gene expression and is related to TRANSCRIPTION FACTOR AP-1. NF-E2 consists of a small MAF protein subunit and a tissue-restricted 45 kDa subunit.
A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A subclass of SOX transcription factors that are expressed in neuronal tissue where they may play a role in the regulation of CELL DIFFERENTIATION. Members of this subclass are generally considered to be transcriptional activators.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
A species of fruit fly much used in genetics because of the large size of its chromosomes.
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
Ubiquitously expressed basic HELIX-LOOP-HELIX MOTIF transcription factors. They bind CANNTG sequences in the promoters of a variety of GENES involved in carbohydrate and lipid metabolism.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
Elements of limited time intervals, contributing to particular results or situations.
A subclass of closely-related SOX transcription factors. Members of this subfamily have been implicated in regulating the differentiation of OLIGODENDROCYTES during neural crest formation and in CHONDROGENESIS.
A family of muscle-specific transcription factors which bind to DNA in control regions and thus regulate myogenesis. All members of this family contain a conserved helix-loop-helix motif which is homologous to the myc family proteins. These factors are only found in skeletal muscle. Members include the myoD protein (MYOD PROTEIN); MYOGENIN; myf-5, and myf-6 (also called MRF4 or herculin).
Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.
Characteristic restricted to a particular organ of the body, such as a cell type, metabolic response or expression of a particular protein or antigen.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II. EC 2.7.7.6.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
This up-regulation is directly controlled by GATA3 transcription factor. IL-33 combined with IL-2, IL-7 or TSLP also stimulates ... These signaling pathways lead to activation of many transcription factors, such as NF-κB, AP-1, c-Jun N-terminal kinase (JNK) ... Its expression is induced by transcription factor NF-κB after exposure of innate immune cells to alarmins. This occurs, for ... The Interleukin-1 family (IL-1 family) is a group of 11 cytokines that plays a central role in the regulation of immune and ...
STAT6 repress the expression of transcription factors T-bet and Foxp3 in TH9 cells, that normally block IL-9 production. GATA-3 ... "The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation". Nature ... Generally IL-1 family members enhance expression of Il9 gene. IL-25 also induces IL-9 production in vivo. Development of TH9 ... There are numbers of cytokines, transcription factors and other molecules, that have a role in TH9 differentiation. Cytokines ...
ROG then dowregulates GATA-3, a characteristic transcription factor for Th2 cells. Tr1 cells express high levels of regulatory ... IL-6 and IL-21 also plays a role in differentiation as they regulate expression of transcription factors necessary for IL-10 ... CD49b belongs to the integrin family and is a receptor for many (extracellular) matrix and non-matrix molecules. CD49b provides ... factors, such as glucocorticoid-induced tumor necrosis factor receptor (GITR), OX40 (CD134), and tumor-necrosis factor receptor ...
... belongs to the GATA family of transcription factors. Gene-deletion studies in mice indicate that Gata3 (mouse gene ... GATA3 is a transcription factor that in humans is encoded by the GATA3 gene. Studies in animal models and humans indicate that ... "Entrez Gene: GATA3 GATA binding protein 3". Zheng, Wei-ping; Flavell, Richard A (May 1997). "The Transcription Factor GATA-3 Is ... that modulate GATA3's gene-stimulating actions. The GATA3 transcription factor regulates the expression of genes involved in ...
Play media GATA transcription factors are a family of transcription factors characterized by their ability to bind to the DNA ... sequence "GATA". In humans: GATA1 (see also GATA1) GATA2 (see also GATA2) GATA3 (see also GATA3) GATA4 (see also GATA4) GATA5 ( ... "DNA-binding specificities of the GATA transcription factor family". Mol. Cell. Biol. 13 (7): 4011-22. doi:10.1128/mcb.13.7.4011 ... GATA+transcription+factors at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ...
Th2 cells, which express IL-4, are inhibited by IL-27 through the transcription factor GATA-3. Th17 cells, which express IL-17 ... Also involved are the STAT1 and STAT3 transcription factors that bind specifically to the receptor subunits, IL-27ɑ and ... Interleukin 27 (IL-27) is a member of the IL-12 cytokine family. It is a heterodimeric cytokine that is composed of two ... Tr1 cells, which express IL-10, are induced by IL-27 through the transcription factor c-Maf. Treg cells are inhibited by IL-27 ...
A well studied pioneer factor family is the Groucho-related (Gro/TLE/Grg) transcription factors that often have a negative ... as is another pioneer factor GATA3. FOXA1 particularly is expressed in 90% of breast cancer metastases and 89% of metastic ... Transcription factors with zinc finger DNA binding domains, such as the GATA family and glucocorticoid receptor. The zinc ... Pioneer factors can function passively, by acting as a bookmark for the cell to recruit other transcription factors to specific ...
The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed ... These transcription factors work with the signaling molecules Wnt1 and Fgf8 to maintain the MHB organizer. The MHB controls ... Pax2 and Pax8 regulate the expression of Gata3. Without these genes mutations in the urogenital system arise. ... RNA polymerase II transcription factor activity, sequence-specific DNA binding. • transcription factor activity, sequence- ...
Geng CD, Vedeckis WV (December 2005). "c-Myb and members of the c-Ets family of transcription factors act as molecular switches ... Transcription factor Spi-B is a protein that in humans is encoded by the SPIB gene. SPI1 (MIM 165170) and SPIB are members of a ... induced Notch1 signaling regulates the human plasmacytoid dendritic cell versus T-cell lineage decision through control of GATA-3 ... Schotte R, Nagasawa M, Weijer K, Spits H, Blom B (December 2004). "The ETS transcription factor Spi-B is required for human ...
"Expression of the T-cell transcription factors, GATA-3 and T-bet, in the neoplastic cells of Hodgkin lymphomas". The American ... T-box transcription factor TBX21, also called T-bet (T-box expressed in T cells) is a protein that in humans is encoded by the ... This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box ... The transcription factor encoded by TBX21 is T-bet, which regulates the development of naive T lymphocytes. Asthma is a disease ...
"Basic helix-loop-helix family, member e41", or BHLHE41, is a gene that encodes a basic helix-loop-helix transcription factor ... BHLH Transcription Factors in Development and Disease. Current Topics in Developmental Biology. bHLH Transcription Factors in ... Gata3 enhances T helper cell 2 (Th2) differentiation signals by regulating BHLHE41 expression through an autoregulatory loop. ... "Phylogenetic and expression analysis of the basic helix-loop-helix transcription factor gene family: genomic approach to ...
... protein encoded by this gene is a widely expressed member of the FOG family of regulators of transcription factors. The family ... For example, it has been shown to bind directly with the N-terminal zinc finger of the GATA4 transcription factor to inhibit ... Interactions with the NuRD complex or a CTBP can cause ZFPM2 to inhibit the ability of GATA3-6 proteins to stimulate the ... Its members may act as coactivators and/or corepressors to modulate the activity of GATA transcription factors. That is, the ...
TH2 and ILC2 cells both express the transcription factor GATA-3, which promotes the production of TH2 cytokines, including the ... families Enzymes, such as elastase Growth factors such as TGF beta, VEGF, and PDGF Cytokines such as IL-1, IL-2, IL-4, IL-5, IL ... Their lineage fate is determined by transcription factors, including GATA and C/EBP. Eosinophils produce and store many ... Horiuchi T, Weller PF (July 1997). "Expression of vascular endothelial growth factor by human eosinophils: upregulation by ...
... and conserved regions Fam158a chromosomal location and neighboring genes transcription factor binding sites in the promoter of ... Human FAM158A and its paralogs in other species are part of the uncharacterized protein family UPF0172 family, which is a ... Several micro-arrays demonstrate the variable expression of Fam158a in response to other factors and in various cancer types. ... "Mutation of GATA3 in human breast tumors". Oncogene. 23 (46): 7669-78. doi:10.1038/sj.onc.1207966. PMID 15361840. Dutta-Simmons ...
"Entrez Gene: forkhead box A1". Bingle CD, Gowan S (1996). "Molecular cloning of the forkhead transcription factor HNF-3 alpha ... and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of ... FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERα− cancers may identify a subset of tumors that is ... These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and ...
... and the general transcription factors TFIIE and TFIIF. Other substrates include the transcription factors Sp1, KLF5, FOXO1, ... Different families of HATs employ unique strategies in order to effect such a transformation. Members of the GNAT family have a ... transcription factor II D), TFTC (TBP-free TAF-containing complex), and NuA3/NuA4 (nucleosomal acetyltransferases of H3 and H4 ... MEF2C, SRY, GATA-4, and HNF-6, HMG-B2, STAT3, the androgen and estrogen (α) receptors, GATA-2, GATA-3, MyoD, E2F(1-3), p73α, ...
... is a transcription factor that belongs to the Signal Transducer and Activator of Transcription (STAT) family of proteins. The ... Similarly as other STAT family proteins, STAT6 is also activated by growth factors and cytokines. STAT6 is mainly activated by ... STAT6 protein also regulates other transctription factor as Gata3, which is important regulator of Th2 differentiation. STAT6 ... STAT6+Transcription+Factor at the US National Library of Medicine Medical Subject Headings (MeSH). ...
IL-5 expression is regulated by several transcription factors including GATA3. Interleukin-5 has long been associated with the ... IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other ... Lopez AF, Begley CG, Williamson DJ, Warren DJ, Vadas MA, Sanderson CJ (May 1986). "Murine eosinophil differentiation factor. An ... "Role of GATA-3 in IL-5 gene transcription by CD4+ T cells of asthmatic patients". Int. Arch. Allergy Immunol. 137 Suppl 1: 55- ...
"Entrez Gene: POU4F3 POU domain, class 4, transcription factor 3". In Vivo Interplay between p27Kip1, GATA3, ATOH1, and POU4F3 ... 1995). "The Brn-3 family of POU-domain factors: primary structure, binding specificity, and expression in subsets of retinal ... 1996). "Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development". Nature. 381 (6583): 603-6 ... POU domain, class 4, transcription factor 3 is a protein that in humans is encoded by the POU4F3 gene. GRCh38: Ensembl release ...
The protein encoded by this gene belongs to the ETS family of transcription factors. ETS2 has been shown to interact with: C- ... "Regulation of the human interleukin-5 promoter by Ets transcription factors. Ets1 and Ets2, but not Elf-1, cooperate with GATA3 ... roles of the Ets transcription factor family". Annals of the New York Academy of Sciences. 1114 (1): 36-47. Bibcode: ... Pastorcic M, Das HK (Nov 2000). "Regulation of transcription of the human presenilin-1 gene by ets transcription factors and ...
Signal transducer and activator of transcription 4 (STAT4) is a transcription factor belonging to the STAT protein family. It ... and signaling factors (MYD88). STAT4 is involved in several autoimmune and cancer diseases in animal models humans, ... "Increased mucosal expression of GATA-3 and STAT-4 in pediatric ulcerative colitis". Pediatrics International. 52 (4): 584-589. ... STAT4+Transcription+Factor at the US National Library of Medicine Medical Subject Headings (MeSH). ...
ILC2s require IL-7 for their development, activating the fundamental transcription factors RORα and GATA3. GATA3 is also ... October 2012). "The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells". Immunity. ... Innate lymphoid cells (ILCs) are the most recently discovered family of innate immune cells, derived from common lymphoid ... their transcription factors, and access of defined chromatin regions to the transcription factors, however, it still remains ...
Wilkinson DA, Neale GA, Mao S, Naeve CW, Goorha RM (January 1997). "Elf-2, a rhombotin-2 binding ets transcription factor: ... Osada H, Grutz G, Axelson H, Forster A, Rabbitts TH (October 1995). "Association of erythroid transcription factors: complexes ... Boehm T, Foroni L, Kaneko Y, Perutz MF, Rabbitts TH (May 1991). "The rhombotin family of cysteine-rich LIM-domain oncogenes: ... induce retinaldehyde dehydrogenase 2 expression in T-cell acute lymphoblastic leukemia by acting as cofactors for GATA3". ...
POU3F1, a factor required for the activation of the transcription factor PU.1. PU.1 negatively regulates GATA3 expression, ... mir-126 is regulated by the binding of two transcription factors: ETS1 and ETS2. Binding of these factors induce the ... Page for mir-126 microRNA precursor family at Rfam. ... Transcription of both occur, however mature mir-126 binds to a ... Sun YQ, Zhang F, Bai YF, Guo LL (2010). "[miR-126 modulates the expression of epidermal growth factor-like domain 7 in human ...
... indicated as a key factor in controlling Th2 cell differentiation and development by stabilizing GATA transcription factors. ... In Ewing sarcoma family of tumors (ESFT), the knockdown of BMI-1 gene would greatly influence the Notch and Wnt signaling ... "Regulation of Th2 cell development by Polycomb group gene bmi-1 through the stabilization of GATA3". J. Immunol. 177 (11): 7656 ... Trimarchi JM, Fairchild B, Wen J, Lees JA (2001). "The E2F6 transcription factor is a component of the mammalian Bmi1- ...
Transcription factors[edit]. NFAT transcription factors are implicated in breast cancer, more specifically in the process of ... "National Research Center for Women & Families.. [permanent dead link]. *^ Light Pollution as new risk factor for human Breast ... GATA-3 directly controls the expression of estrogen receptor (ER) and other genes associated with epithelial differentiation, ... Prognostic factors[edit]. Prognostic factors are reflected in the classification scheme for breast cancer including stage, (i.e ...
"Basic helix-loop-helix family, member e41", or BHLHE41, is a gene that encodes a basic helix-loop-helix transcription factor ... bHLH Transcription Factors in Development and Disease. 110. Academic Press. pp. 317-338. doi:10.1016/B978-0-12-405943-6.00009-9 ... Gata3 enhances T helper cell 2 (Th2) differentiation signals by regulating BHLHE41 expression through an autoregulatory loop.[ ... RNA polymerase II transcription factor binding. • RNA polymerase II activating transcription factor binding. • RNA polymerase ...
The transcription factor gene GATA3 is altered in mammary tumors. Down-regulation of GATA3 expression and activity by the Zinc- ... "Regulation of the Development and Function of B Cells by ZBTB Transcription Factors". Frontiers in Immunology. 9: 580. doi: ... It is a member of the Poxviruses and Zinc-finger (POZ) and Krüppel (POK) family of proteins, and was identified in multiple ... Ikeda R, Yoshida K, Inoue I (May 2007). "Identification of FAZF as a novel BMP2-induced transcription factor during ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ... GATA-3 belongs to the GATA transcription factor family, which consists of a group of vertebrate GATA factors that have two ... Family relationships *Transcription factor GATA (IPR039355) *Transcription factor, GATA-2/3 (IPR016374) *Transcription factor ... GATA-3 is a trans-acting T-cell-specific transcription factor required for several developmental steps in T-cells [PMID: ...
Transcription factor HEB (59) is an E-box protein belonging to the bHLH family. HEB, like TCF-1, has been implicated in the ... Previous studies showed that Gata3 is regulated by transcription factors HEB (37, 38), T cell factor 1 (TCF-1) (39), and RBP-J/ ... The transcription factor GATA3 is essential for the genesis and maturation of the T cell lineage, and GATA3 dysregulation has ... TCF-1, HEB, and RBP-J regulate Gata3 transcription by binding within Tce1. Several transcription factors have been proposed as ...
From the Gata family of transcription factors, only Gata2 and Gata3 are found in the nervous system in overlapping expression ... The transcription factor Gata3 is essential for the development of sympathetic neurons and adrenal chromaffin cells. As Gata3 ... The Gata3 Transcription Factor Is Required for the Survival of Embryonic and Adult Sympathetic Neurons. Konstantina Tsarovina, ... 2001) Transcription factor GATA-3 alters pathway selection of olivocochlear neurons and affects morphogenesis of the ear. J ...
1990) Activity and tissue specific expression of the transcription factor NF-E1 [GATA] multigene family. Genes Dev 4, 1650-1662 ... 1999) Expression and genetic interaction of transcription factors GATA-2 and GATA-3 during development of the mouse central ... we show that the transcription factor GATA3 is dynamically expressed during hindbrain development. Function of GATA3 in ventral ... 1997) The transcription factor GATA-3 is necessary and sufficient for Th2 cytokine gene expression in CD4 T cells. Cell 89, 587 ...
We identified GATA-3 as the most highly enriched transcription factor in the mammary epi … ... The GATA family of transcription factors plays fundamental roles in cell-fate specification. However, it is unclear if these ... The GATA family of transcription factors plays fundamental roles in cell-fate specification. However, it is unclear if these ... We identified GATA-3 as the most highly enriched transcription factor in the mammary epithelium of pubertal mice. GATA-3 was ...
GATA3 belongs to the GATA family of transcription factors. Gene-deletion studies in mice indicate that Gata3 (mouse gene ... GATA3 is a transcription factor that in humans is encoded by the GATA3 gene. Studies in animal models and humans indicate that ... "Entrez Gene: GATA3 GATA binding protein 3". Zheng, Wei-ping; Flavell, Richard A (May 1997). "The Transcription Factor GATA-3 Is ... that modulate GATA3s gene-stimulating actions. The GATA3 transcription factor regulates the expression of genes involved in ...
GATA family of transcription factors 3, a Th2 master regulator (GATA3), Foxp3, cytotoxic T-lymphocyte-associated antigen 4 ( ... tumor necrosis factor; Dex, dexamethasone; GR, glucocorticoid receptor; IB, immunoblot; GATA3, GATA family of transcription ... A representative immunoblot (IB) of the relevant Th1 or Th2 transcription factors T-bet (A) and GATA3 (C) are shown. T-bet: 1, ... DC-derived factors, including augmentation of the transcription factor T-bet following activation by DC-derived IL-12. The ...
GATA family of transcription factors 3, a Th2 master regulator (GATA3), Foxp3, cytotoxic T-lymphocyte-associated antigen 4 ( ... tumor necrosis factor; Dex, dexamethasone; GR, glucocorticoid receptor; IB, immunoblot; GATA3, GATA family of transcription ... A representative immunoblot (IB) of the relevant Th1 or Th2 transcription factors T-bet (A) and GATA3 (C) are shown. T-bet: 1, ... DC-derived factors, including augmentation of the transcription factor T-bet following activation by DC-derived IL-12. The ...
Complete information for GATA3 gene (Protein Coding), GATA Binding Protein 3, including: function, proteins, disorders, ... Entrez Gene Summary for GATA3 Gene. * This gene encodes a protein which belongs to the GATA family of transcription factors. ... Targeted motifs for GATA3 Gene. HOMER Transcription Factor Regulatory Elements motif GATA3 * Consensus sequence: AGATAASR ... View all 14 R&D Systems GATA3 (GATA-3) Products. *View all 14 R&D Systems GATA3 (GATA-3) Primary Antibodies*Human GATA-3 ...
Ko, L. J. and Engel, J. D. (1993). DNA-binding specificities of the GATA transcription factor family. Mol. Cell. Biol. 13,4011 ... To address the consequences of Gata3 deficiency on other SA cell-specific transcription factors, we enriched lacZ+ E18.5 ... Gata3 encodes a transcription factor containing two steroid hormone receptor-like zinc fingers that serve as its DNA-binding ... Of the six Gata factor family members, only Gata2 and Gata3 are reportedly expressed in the nervous system (Nardelli et al., ...
This up-regulation is directly controlled by GATA3 transcription factor. IL-33 combined with IL-2, IL-7 or TSLP also stimulates ... These signaling pathways lead to activation of many transcription factors, such as NF-κB, AP-1, c-Jun N-terminal kinase (JNK) ... Its expression is induced by transcription factor NF-κB after exposure of innate immune cells to alarmins. This occurs, for ... The Interleukin-1 family (IL-1 family) is a group of 11 cytokines that plays a central role in the regulation of immune and ...
Compare and order GATA3 ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended ... This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc ... transcription factor GATA-3 , GATA binding factor-3 , transcription factor xGATA-3 , GATA-3 , NF-E1 , transcription factor NF- ... Bezeichner auf Proteinebene für GATA3 GATA-binding factor 3 , trans-acting T-cell-specific transcription factor GATA-3 , GATA- ...
Importantly, the transcription factor NFAT4, thought to act in part as a suppressor of IL-4 transcription (29, 30), was ... Based on this data, the discordant regulation of other genes such as transcription factors might be predicted to be important ... These included GATA3, STAT1, STAT4, JunB, JunD, and NFAT4. Notably, JunB and GATA3 recently were reported to be preferentially ... and the tec family kinase, Itk (20-23). These transcripts were found at significantly greater abundance in the IL-4+ clone. ...
... a member of the GATA family of transcription factors. Like other members of the family, GATA3 contain zinc finger motifs ... Primary function attributed to GATA3 is to regulate the T helper 2 (Tʜ2) cell differentiation. However, role of GATA3 is also ... Expression of GATA3 is found in developing and mature T cells, natural killer (NK) cells, subset of NKT cells, and endothelial ... GATA3 has two trans-activating domains and two zinc finger domains, each followed by a conserved basic region. ...
FOG Family Member 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and ... DNA-binding transcription factor activity, RNA polymerase II-specific. ISA. --. GO:0001085. RNA polymerase II transcription ... GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter ...
Compare and order GATA3 ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended ... GATA3 Antigen Profile Antigen Summary This gene encodes a protein which belongs to the GATA family of transcription factors. ... transcription factor GATA-3 , GATA binding factor-3 , transcription factor xGATA-3 , GATA-3 , NF-E1 , transcription factor NF- ... B. Transcription factor activity assay of GATA-3 from nuclear fractions with the GATA-3 Transcription Factor-Activity Assay Kit ...
... a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic ... Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(cat ... During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is ... transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box ...
PD-1 engagement prevents the expression of transcription factors associated with effector cell function, including GATA-3, T- ... The transcription factor FoxO1 sustains expression of the inhibitory receptor PD-1 and survival of antiviral CD8(+) T cells ... Lack of SHPTP1 results in src-family kinase hyperactivation and thymocyte hyperresponsiveness. Proc Natl Acad Sci U S A (1996) ... Transcription factor T-bet represses expression of the inhibitory receptor PD-1 and sustains virus-specific CD8+ T cell ...
There are 6 transcription factors in the GATA family: GATA1 to GATA6. GATA2 is a transcriptional activator regulating ... transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding zinc ion binding C2H2 ... PA1-100 specifically detects GATA2 and does not cross-react with GATA1 or GATA3. ... GATA2 (GATA binding protein 2) belongs to the GATA family of transcription factors. GATA proteins play a very important role in ...
The zinc-finger GATA3 transcription factor has been reported to be involved in the growth control and differentiation of breast ... This review focuses on the immunohistochemical expression of members of the MUC-type mucin family in salivary gland tumors and ... We have characterized the mouse Muc3 5 structure and regulation of its promoter by cytokines and growth factors. The first two ... GATA3 and MUC1 expression was analyzed in vitro with a GATA3 knockdown assay. Furthermore, expression of GATA3 and MUC1 genes ...
Blokzijl et al. report that, in immune cells, TGF-β induces the Smad3 assocation with GATA-3, a transcription factor whose ... Therefore, the wide range and tissue-specific effects of TGF-β-like factors are not well understood. ... family has been implicated in regulating cell-fate decisions in the immune and nervous systems. Expression of Smad3 in a T cell ... Overexpression of dominant negative forms of either Smad3 or GATA-3 in a T cell line inhibited cytokine production. Hence, GATA ...
GATA6 is a member of a transcription factor family that can be subdivided into 2 groups. GATA1, GATA2, and GATA3 are involved ... Several transcription factors have been implicated during these stages in the early embryo, including the master regulator ... Activity and tissue-specific expression of the transcription factor NF-E1 multigene family. Genes Dev. 1990;4(10):1650-1662. ... GATA-6 activates transcription of thyroid transcription factor-1. J Biol Chem. 1999;274(5):2658-2664.. View this article via: ...
Two families of nuclear transcription factors-the GATA NITRATE-INDUCIBLE CARBON-METABOLISM-INVOLVED (GNC) and GOLDEN TWO-LIKE ( ... and GATA3 family genes in Arabidopsis. Plant Cell 25: 83-101. ... The GNC family of GATA transcription factors is implicated in ... Two families of nuclear transcription factors, the GATA NITRATE-INDUCIBLE CARBON-METABOLISM-INVOLVED (GNC) and GOLDEN TWO-LIKE ... Coordination of Chloroplast Development through the Action of the GNC and GLK Transcription Factor Families. Yan O. Zubo, Ivory ...
Among its other actions, STAT6 promotes transcription of GATA3 (a Th2 cell inducer) and MHCII (myeloid and B cells), and ... as IL-4 can also elicit astrocytic expression of other CNS growth factors, such as nerve growth factor (38, 39). This function ... The type 2 complex also responds to IL-13, and it can signal other STAT molecules (i.e., STAT3) through the JAK family kinase ... and traffics to the nucleus to function as a transcription factor for Th2, IgE, and AAMΦ-associated genes. The type 1 receptor ...
The transcription factor GATA-3 controls cell fate and maintenance of type 2 innate lymphoid cells. Immunity 37, 634-648 (2012 ... ILC2 differentiation is dependent on the transcription factors GATA36 and RORα7. Once activated by alarmins (e.g., IL-33, IL-25 ... Recently, some members of the PG family, namely PGI2, PGD2 and PGE2, have emerged as key regulators of ILC2 and ILC3 functions ... Transcription factor RORalpha is critical for nuocyte development. Nat. Immunol. 13, 229-236 (2012). ...
Transcriptional activator which probably serves as a general switch factor for cell-specific development. It binds to DNA sites ... R-GGA-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs. R-GGA-983231. Factors involved in ... Family and domain databases. Gene3D Structural and Functional Annotation of Protein Families ... sp,P23825,GATA3_CHICK GATA-binding factor 3 OS=Gallus gallus GN=GATA3 PE=2 SV=1 ...
Transcription factor GATA-3 is required for development of the T-cell lineage. Nature 1996;384(6608):474-478. ... GATA2 belongs to a family of zinc finger transcription factors that are critical regulators of gene expression in hematopoietic ... Regulation of trophoblast-specific factors by GATA2 and GATA3 in bovine trophoblast CT-1 cells. J Reprod Dev 2011;57(4):518-525 ... Human transcription factor GATA-2. Evidence for regulation of preproendothelin-1 gene expression in endothelial cells. J Biol ...
  • From the Gata family of transcription factors, only Gata2 and Gata3 are found in the nervous system in overlapping expression patterns, with important roles in the development of specific neuronal populations, including neurons in the inner ear, midbrain, hindbrain, spinal cord, and sympathoadrenal system. (jneurosci.org)
  • Ubiquitous expression of Hoxb1 in the hindbrain induces ectopic expression of GATA2 and GATA3 in ventral r2 and r3. (biologists.org)
  • These findings demonstrate that GATA2 and GATA3 lie downstream of Hoxb1 and provide the first example of Hox pathway transcription factors within a defined population of vertebrate motor neurons. (biologists.org)
  • Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. (genecards.org)
  • Other transcription factors such as SP1, GATA2 and GATA3 were also found to significantly stimulate Syncytin-1 promoter activity [24]. (immune-source.com)
  • GATA-3 belongs to the GATA transcription factor family, which consists of a group of vertebrate GATA factors that have two highly conserved zinc finger motifs and recognise a six-nucleotide motif T/AGATAG/A in target genes. (ebi.ac.uk)
  • In the Gata3-deficient population, the expression of anti-apoptotic genes ( Bcl-2 , Bcl-x L , and NF κ B ) is diminished, whereas the expression of pro-apoptotic genes ( Bik , Bok , and Bmf ) was increased. (jneurosci.org)
  • Specification of neuron fate is mediated by networks of transcription factors that play essential roles in activating target genes of specific neuron fates ( Guillemot, 2007 ). (jneurosci.org)
  • GATA3 haploinsufficiency (i.e. loss of one or the two inherited GATA3 genes) results in a congenital disorder termed the Barakat syndrome. (wikipedia.org)
  • Knockout of both GATA3 genes in mice is fatal: these animals die at embryonic days 11 and 12 due to internal bleeding. (wikipedia.org)
  • The GATA3 transcription factor regulates the expression of genes involved in the development of various tissues as well as genes involved in physiological as well as pathological humoral inflammatory and allergic responses. (wikipedia.org)
  • However, SA lineage-specific restoration of Gata3 function in the Gata3 mutant background rescues the expression phenotypes of the downstream, as well as the putative upstream genes. (biologists.org)
  • Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. (thermofisher.com)
  • ChIP-seq analysis of GNC identified PHYTOCHROME INTERACTING FACTOR and brassinosteroid activity genes as targets whose repression by GNC facilitates chloroplast biogenesis. (plantphysiol.org)
  • Because some ERα target genes include transcription factors and regulatory proteins, a secondary cascade of gene expression is triggered following the initial stimulation of ERα. (mcponline.org)
  • Appearance and disappearance of Syk family protein-tyrosine kinase genes during metazoan evolution. (antievolution.org)
  • Igf1 , Tgfbr1 ), as well as genes for various transcription factors and signalling proteins. (biologists.org)
  • Recent studies have demonstrated that members of the GATA-binding protein (GATA) family (GATA4 and GATA5) might have pivotal roles in the transcriptional upregulation of mucin genes ( MUC2 , MUC3 and MUC4 ) in gastrointestinal epithelium. (beds.ac.uk)
  • Furthermore, expression of GATA3 and MUC1 genes was analyzed by real-time RT-PCR and immunohistochemistry on breast cancer-specific tissue microarrays. (beds.ac.uk)
  • In addition, we detected a statistically significant correlation in expression between GATA3 and MUC1 genes at the mRNA and protein levels both in normal breast epithelium and in breast carcinomas ( p = 0.01). (beds.ac.uk)
  • Además, se piensa que las interacciones entre los componentes de la soja, como los aminoácidos estándar, la grasa poliinsaturada y la fracción enriquecida en isoflavonoides, mejoran la oxidación de los ácidos graos en el parénquima hepático al aumentar la expresión de los genes regulados por el receptor α activado por el proliferador del peroxisoma (PPARα), disminuyendo así la acumulación de lípidos en el hígado. (isciii.es)
  • [9] Similar patterning during embryological development can be observed in "basal chordates or ascidians," in which organization of the central nervous system in ascidian larvae are also controlled by fibroblast growth factor genes. (wikipedia.org)
  • Interleukin-17 (IL-17) and IL-25 signaling induce the expression of genes encoding inflammatory factors and are implicated in the pathology of various inflammatory diseases. (jove.com)
  • Nuclear factor ?B (NF-?B) activator 1 (Act1) is an adaptor protein and E3 ubiquitin ligase that is critical for signaling by either IL-17 or IL-25, and it is recruited to their receptors (IL-17R and IL-25R) through heterotypic interactions between the SEFIR [SEF (similar expression to fibroblast growth factor genes) and IL-17R] domain of Act1 and that of the receptor. (jove.com)
  • We have isolated human and murine genes of NFAT, a transcription factor that plays a critical role in transcriptional induction of cytokine genes. (nii.ac.jp)
  • This involved calcineurin/transcription factor EB (TFEB) pathway and its target genes, cathepsin B, cathepsin D, and LAMP-1, to mediate lysosomal exocytosis. (antibody-antibodies.com)
  • In DNA microarrays of human umbilical vein endothelial cells (HUVEC), small interfering RNA-mediated knockdown of GATA3 resulted in reduced expression of genes associated with angiogenesis, including Tie2. (uniprot.org)
  • In breast cancer, some GATA3 effects shift from tumor suppressing to tumor promoting during tumorigenesis, with deregulation of three genes, BCL2, DACH1, THSD4, representing major GATA3-controlled processes in cancer progression. (antikoerper-online.de)
  • These rearrangements will juxtapose one of these T-cell receptor genes with many critical partner genes, many of which code for transcription factors. (usal.es)
  • These mutations are found in genes involved in the JAK-STAT and Ras/PI3K/AKT pathways, in epigenetic regulation, in mRNA maturation and ribosome activity, in modifying histone methylation or acetylation, and that function as regulators of transcription. (usal.es)
  • These data not only underscore the hypothesis that Gata3 is essential for the differentiation and survival of SA cells, but also suggest that their differentiation is controlled by mutually reinforcing feedback transcriptional interactions between Gata3, Mash1, Hand2 and Phox2b in the SA lineage. (biologists.org)
  • Transcriptional activator which probably serves as a general switch factor for cell-specific development. (uniprot.org)
  • Our study provides evidence indicating that GATA3 is probably a mediator for the transcriptional upregulation of MUC1 expression in some breast cancers. (beds.ac.uk)
  • The function of GATA factors is modulated by their interaction with other transcription factors, transcriptional coactivators and co-repressors. (beds.ac.uk)
  • In addition to its function in thymus, TCF1 promotes T cell differentiation to Th2 cells in the periphery through transcriptional activation of GATA3. (biolegend.com)
  • We analyzed the 3-dimensional structure and transcriptional function of this mutant GATA3. (biomedcentral.com)
  • Transcriptional inactivation and CpG island (CGI) methylation of GATA3 and GATA5, two members of the GATA family of transcription factors, have been observed in some human cancers. (readbyqxmd.com)
  • This motif is important because it is recognized by the GATA family transcription factors (see [ 1 ] and references therein), which are the transcriptional regulators of NCR in Saccharomyces cerevisiae . (springer.com)
  • These results demonstrate that Gata3 is continuously required for maintaining survival but not differentiation in the sympathetic neuron lineage up to mature neurons of adult animals. (jneurosci.org)
  • In Gata3 -null embryos, there was significant impairment of SA differentiation and increased apoptosis in adrenal chromaffin cells and sympathetic neurons. (biologists.org)
  • We show that Gata3 is essential not only for Th and Dbh activation, but also for cell survival and differentiation of sympathetic neurons and adrenal chromaffin cells. (biologists.org)
  • Primary function attributed to GATA3 is to regulate the T helper 2 (Tʜ2) cell differentiation. (miltenyibiotec.com)
  • Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. (genecards.org)
  • Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. (thermofisher.com)
  • Estrogen receptor-alpha (ERα) 1 belongs to the family of transcription factors which control cell growth and differentiation and regulate the expression of proto-oncogenes. (mcponline.org)
  • We found that modulation of WNT and transforming growth factor-β (TGF-β) signaling combined with fibroblast growth factor 3 (FGF3) and FGF10 treatment over a 6-day period drives the expression of early otic/placodal markers followed by late otic sensory markers within 13 days, indicative of a differentiation into embryonic-like HCs. (frontiersin.org)
  • The zinc-finger GATA3 transcription factor has been reported to be involved in the growth control and differentiation of breast epithelial cells. (beds.ac.uk)
  • Schematic view of the differentiation of various innate lymphoid cells (ILCs), of the factors involved in such a differentiation, and of the cytokines produced by distinct subsets of ILCs. (hindawi.com)
  • NFIL3 is a transcription factor that regulates a variety of cellular processes in lymphocyte subsets including cytokine production, class switching, and cell differentiation. (biolegend.com)
  • The present study revealed that Th2 cells may be an indispensable intermediate during the differentiation of Th0 cells into Th9 cells, which depends on the activation of the Smad3/Smad4 and interferon-regulatory factor 4 (IRF-4) pathways. (spandidos-publications.com)
  • On the other hand, GATA3 also serves as a master regulator of the differentiation of T helper type 2 cells [ 5 , 6 ], but no immune deficient symptom has been reported in patients with HDR syndrome. (biomedcentral.com)
  • We now know that T H 1 differentiation is regulated by transcription factors such as T-bet, Stat1, and Stat4, as well as cytokines such as IL-12, IL-23, IL-27, type I IFNs, and IFN-γ. (springer.com)
  • The transcription factors Stat6, GATA-3, c-Maf, NFATs, and the cytokine IL-4 promote T H 2 differentiation. (springer.com)
  • We found that NFAT family mRNAs are developmentally regulated during the differentiation of T cells in the thymus, and that NFATx protein selectively contributes to the calcineurin-dependent NFAT-DNA binding activity in the DP stage, suggesting that NFAT is also involved in the signals required for generation of T cells in the thymus. (nii.ac.jp)
  • GATA transcription factors play an essential role in the developmental control of cell fate, cell proliferation and differentiation, organ morphogenesis, and tissue-specific gene expression. (biomedcentral.com)
  • Previous studies have shown that GATA3 function in T cell development is regulated by multiple signaling pathways and that the Notch nuclear effector, RBP-J, binds specifically to the Gata3 promoter. (jci.org)
  • Gata3 mutant mice expire of noradrenergic deficiency by embryonic day (E) 11 and can be rescued pharmacologically or, as shown here, by restoring Gata3 function specifically in sympathoadrenal (SA) lineages using the human DBH promoter to direct Gata3 transgenic expression. (biologists.org)
  • Promoter sequence analysis of the MUC1 gene identified six GATA cis consensus elements in the 5' flanking region (GATA1, GATA3 and four GATA-like sequences). (beds.ac.uk)
  • We confirmed the presence of a functional GATA3-binding site on the MUC1 promoter region in the MCF7 cell line. (beds.ac.uk)
  • Functional analysis of this mutant protein using an in vitro luciferase reporter assay system confirmed that the mutation abolished the enhancing effects of wild-type GATA3 on the promoter activity of the consensus GATA responsive element and that of human PTH gene. (biomedcentral.com)
  • Pathologically, Pax2 has been demonstrated to activate hepatocyte growth factor (HGF) gene promoter, and both have been indicated as playing a role in human prostate cancers. (wikipedia.org)
  • In co-immunoprecipitation and co-transfection assays, GATA3 and the Ets transcription factor, ELF1, physically interacted and synergized to transactivate the Tie2 promoter. (uniprot.org)
  • The results suggest that HDR syndrome is primarily caused by GATA3 haploinsufficiency and is associated with a wide phenotypic spectrum. (bmj.com)
  • However, GATA3 haploinsufficiency has been reported in only five families with a fairly typical HDR phenotype, 10 so that the frequency of GATA3 haploinsufficiency and the phenotypic spectrum remain to be determined. (bmj.com)
  • These results are consistent with the predicted functions of human GATA3-ZnF1 from three-dimensional molecular modeling and with HDR being a result of GATA3 haploinsufficiency. (ox.ac.uk)
  • GATA3 variant 1 is a linear protein consisting of 444 amino acids. (wikipedia.org)
  • GATA3 variant 2 protein is an identically structured isoform of, but 1 amino acid shorter than, GATA3 variant 1. (wikipedia.org)
  • The other zinc finger, ZNF1, is at the protein's N-terminus and interacts with various nuclear factors, including Zinc finger protein 1 (i.e. (wikipedia.org)
  • This gene encodes a protein which belongs to the GATA family of transcription factors. (genecards.org)
  • GATA3 (GATA Binding Protein 3) is a Protein Coding gene. (genecards.org)
  • Transcription factors that mediate BMP signaling in specifying the SA lineage have been identified and include the zinc-finger protein, Gata3, the bHLH proteins Mash1 (Ascl1 - Mouse Genome Informatics) and Hand2, as well as the closely related homeodomain transcription factors Phox2a and Phox2b. (biologists.org)
  • All of the members of IL-1 family, except IL-1Ra, are first synthesized as a precursor protein, which means it is synthesized as a long form of a protein which has to be proteolytically cleaved to a shorter, active molecule, which is generally called a mature protein. (wikipedia.org)
  • ZFPM1 (Zinc Finger Protein, FOG Family Member 1) is a Protein Coding gene. (genecards.org)
  • GATA2 (GATA binding protein 2) belongs to the GATA family of transcription factors. (thermofisher.com)
  • Finally, we quantified dynamics of protein expression in a rarely studied network of transcription factors with a negative feedback loop (ERα-EGR3-NAB2). (mcponline.org)
  • Because NAB2 protein is a repressor of EGR3-induced transcription, siRNA-mediated silencing of NAB2 resulted in the enhanced expression of the EGR3-induced protein ITGA2. (mcponline.org)
  • ERα action is exerted through four distinct pathways: direct ligand-dependent transcription through binding to estrogen response elements (EREs), tethered mode through its binding to other transcription factors which interact with their DNA response elements, nongenomic pathway mediated through membrane or cytoplasmic ERα and rapid signaling by protein kinases, and finally, estrogen-independent pathway through growth factor signaling and ERα phosphorylation ( 2 ). (mcponline.org)
  • We determined that GATA3 knockdown assays led to a decrease in MUC1 protein expression in MCF7 and T47D cells. (beds.ac.uk)
  • GATA3 (GATA-binding protein 3) belongs to a family of transcription factors (GATA1 to GATA6) that bind with high affinity to the consensus sequence (A/T)GATA(A/G) and share a steroid-hormone-receptor superfamily C4 zinc-finger DNA-binding motif [ 1 ]. (beds.ac.uk)
  • Trans-acting T-cell-specific transcription factor GATA-3 is a protein that in humans is encoded by the GATA3 gene. (chemical-reagent.com)
  • These complexities arise in part from multiple controls on surface coreceptor expression including protein turnover and mRNA stability, as well as transcription ( 24 , 25 ). (rupress.org)
  • Soy intake decreases the expression of sterol regulatory-element binding protein-lc (SREBP-1) and increases the expression of SREBP-2, which are transcription factors associated with the regulation of hepatic lipogenesis and reduction of cholesterol synthesis and absorption in the liver, respectively. (isciii.es)
  • A gene on chromosome 10p15 that encodes a zinc finger protein belonging to the GATA family of transcription factors, which is a key regulator of T-cell development and plays an important role in endothelial cell biology. (thefreedictionary.com)
  • Maintenance of the syncytiotrophoblast structure relies on newly fused cytotrophoblasts, a process that is regulated by different transcription factors, growth factors, cytokines, protein kinases and fusogenic proteins such as former envelope (Env) glycoproteins of human Endogenous Retroviruses (ERVs) Syncytin-1 of ERVW-1 and Syncytin-2 of ERVFRD-1. (immune-source.com)
  • The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set. (fishersci.se)
  • Generalized linear models were used to assess FOXA1 protein expression in relation to reproductive factors by ER status. (aacrjournals.org)
  • IKKi deficiency abolished IL-17-induced formation of the complex of Act1 and the adaptors TRAF2 and TRAF5, activation of mitogen-activated protein kinases (MAPKs) and mRNA stability, whereas the Act1-TRAF6-transcription factor NF-?B axis was retained. (jove.com)
  • The protein is a member of the GATA family of transcription factors, which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' ( uniprot P15976 ). (bio-rad-antibodies.com)
  • Immune checkpoint factors, such as programmed cell death protein-1/2 (PD-1, PD-2) or cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) receptors, are targets for monoclonal antibodies (MAbs) developed for cancer immunotherapy. (biomedcentral.com)
  • In 1985 two distinct, but distantly related complementary DNAs encoding proteins sharing human IL-1 activity were reported to be isolated from a macrophage cDNA library, thus defining two individual members of the IL-1 family - IL-1α and IL-1β. (wikipedia.org)
  • The MUC1 glycoprotein is a member of the mucin family of proteins, expressed mostly on the apical membrane of various glandular epithelia such as in luminal breast epithelial cells [ 16 ]. (beds.ac.uk)
  • A high expression of the phosphorylated signal transducers and activators of transcription (STAT) proteins 1 and 5 were found at the margin of the structures. (arvojournals.org)
  • Identification of enhancer sets predicted to be co-regulated by the same transcription factor. (biomedcentral.com)
  • Identification of activated enhancers and linked transcription factors in breast, prostate, and kidney tumors by tracing enhancer networks using epigenetic traits Epigenetics Chromatin. (usc.edu)
  • The C_EBP family of transcription factors regulates viral and cellular CCAAT_enhancer element-mediated transcription. (antibody-antibodies.com)
  • Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set. (thermofisher.com)
  • Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. (thermofisher.com)
  • Objective: The aim of this study was to assess the balance of CD4(+) T cell populations in relapsing-remitting MS. Methods: Blood mRNA expression of TBX21, GATA3, RORC, FOXP3 and EBI3 was assessed in 33 patients with relapsing-remitting MS and 20 healthy controls. (thisisms.com)
  • This dual zinc finger transcription factor binds DNA with its C-terminal zinc finger (ZnF2) and stabilizes this binding with its N-terminal zinc finger (ZnF1). (ox.ac.uk)
  • Several transcription factors have been implicated during these stages in the early embryo, including the master regulator thyroid transcription factor 1 (also known as NKX2.1). (jci.org)
  • At the molecular level, we report that IL-25-mediated induction of Th2 master regulator GATA-3 and the transcription factor GFI-1 is attenuated in Act1-deficient T cells. (jove.com)
  • Hypoparathyroidism, deafness, and renal dysplasia (HDR) syndrome is a rare autosomal dominant disorder caused by mutations in the zinc finger transcription factor gene, GATA3 . (biomedcentral.com)
  • Sequence analysis showed heterozygous novel mutations in three families: a missense mutation within the first zinc finger domain at exon 4 (T823A, W275R), an unusual mutation at exon 4 (900insAA plus 901insCCT or C901AACCCT) resulting in a premature stop at codon 357 with loss of the second zinc finger domain, and a nonsense mutation at exon 6 (C1099T, R367X). (bmj.com)
  • GATA3 mutations cause hypoparathyroidism with sensorineural deafness and renal dysplasia. (thefreedictionary.com)
  • The hypoparathyroidism, deafness, and renal dysplasia (HDR) syndrome is an autosomal dominant disorder caused by mutations of a member of the GATA-binding family of transcription factors, GATA3. (ox.ac.uk)
  • that binds most avidly to the consensus motif AGATCTTA ( Ko and Engel, 1993 ) and is highly conserved among all six members (Gata1 to Gata6) of this multigene family ( Patient and McGhee, 2002 ). (biologists.org)
  • Rabbit anti GATA3 antibody recognizes GATA3, also known as Trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3. (bio-rad-antibodies.com)
  • The development of Th subpopulations is dependant on the expression of lineage-specific transcription factors. (thisisms.com)
  • A bioinformatics analysis of these data was performed focusing on transcription factors, immunoglobulin superfamily members, and lineage-specific transcripts. (bloodjournal.org)
  • There are 6 transcription factors in the GATA family: GATA1 to GATA6. (thermofisher.com)
  • Mouse anti Human GATA3 antibody recognizes the transcription factor GATA-3, also known as GATA-binding factor 3 and trans-acting T-cell-specific transcription factor GATA-3. (bio-rad-antibodies.com)
  • Like other members of the family, GATA3 contain zinc finger motifs involved in recognizing the canonical GATA DNA sequence. (miltenyibiotec.com)
  • Our results define key regulatory features of the GNC and GLK transcription factor families that contribute to the control of chloroplast biogenesis and photosynthetic activity, including areas of independence and cross talk. (plantphysiol.org)
  • Collections of transcription factor binding profiles (Transfac, Jaspar) are essential to identify regulatory elements in DNA sequences. (biomedcentral.com)
  • Simultaneously, the levels of interferon‑regulatory factor 4 (IRF‑4) and Smad3/Smad4 were significantly increased following Th9‑cell polarization. (spandidos-publications.com)
  • Etv5 deficiency did not affect the expression of other transcription factors known to be important for expression of IL-10, including Jun family members, GATA3, E4BP4, and IFN regulatory factor 4. (elsevier.com)
  • Ectopic Etv5 expression in Th2 cells that lack Etv5 restored IL-10 production and the binding of IL-10-inducing transcription factors including E4BP4, IFN regulatory factor 4, and GATA3. (elsevier.com)
  • Antp is a sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. (antibody-antibodies.com)
  • In electrophoretic gel mobility shift assays and chromatin immunoprecipitation, GATA3 was shown to bind to regulatory regions within the 5'-untranslated region of the Tie2 gene. (uniprot.org)
  • In SAGE (serial analysis of gene expression) studies we observed an intriguing significant correlation between GATA3 and MUC1 mRNA expression in breast carcinomas. (beds.ac.uk)
  • The mRNA expression levels of matrix metalloproteinase 2 (MMP-2) and tissue inhibitor of metalloproteinase 1, 2 (TIMP1, 2) were measured using quantitative real-time reverse transcription-polymerase chain reaction. (bvsalud.org)
  • The Interleukin-1 family (IL-1 family) is a group of 11 cytokines that plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. (wikipedia.org)
  • IL-1 family is a group of 11 cytokines, which induces a complex network of proinflammatory cytokines and via expression of integrins on leukocytes and endothelial cells, regulates and initiates inflammatory responses. (wikipedia.org)
  • Specifically, ILCs1 express the transcription factor T-bet and secrete T helper type-1- (Th1-) related cytokines, ILCs2 are dependent on the transcription factor ROR α and express Gata-3 and the chemokine receptor homologous molecule (CRTH2) and produce Th2-related cytokines, and ILCs3 express the transcription factor ROR γ t and synthesize interleukin- (IL-) 17, IL-22, and, under specific stimuli, interferon- γ . (hindawi.com)
  • In current study was analyzed factors that facilitate the Th-subset specific expression of cytokines. (nii.ac.jp)
  • In the SG of Gata3 mutants, normal expression of Phox2 and other neuronal markers, but not Th or Dbh, was detected, suggesting that Gata3 acts genetically downstream of Phox2 and is required only for the expression of terminal noradrenergic traits. (biologists.org)
  • We have previously reported the critical role of the U-box-type E3 ubiquitin ligase Act1 (1) for the downstream signaling of the IL-17 cytokine family including the Th2-promoting cytokine IL-25 (IL-17E) (2). (jove.com)
  • There is increasing evidence that the GATA family of transcription factors function as signal transducers, coupling changes in the extracellular environment to changes in downstream target gene expression. (uniprot.org)
  • T1/ST2 is an IL-1 receptor family member originally identified in murine fibroblasts 19 20 . (pubmedcentralcanada.ca)
  • The transcription factor Gata3 is essential for the development of sympathetic neurons and adrenal chromaffin cells. (jneurosci.org)
  • In contrast to drug-rescued Gata3 mutants, SA tissue-specific Gata3 complementation restored nearly normal development of sympathetic neurons and neuroendocrine chromaffin cells as well as of Mash1, Hand2 and Phox2b expression. (biologists.org)
  • 1999 ) The transcription factor GATA6 is essential for early extraembryonic development. (biologists.org)
  • The transcription factor GATA6 has been shown to be important for lung development and branching morphogenesis in mouse models, but its role in human lung development is largely unknown. (jci.org)
  • One such pair is shown as a scatter plot of all breast cancer (BRCA) tumor and adjacent normal samples, for the GATA motif and the GATA3 TF. (biomedcentral.com)
  • GATA3 expression was also highly associated with estrogen receptor and progesterone receptor status ( p = 0.0001) and tumor grade ( p = 0.004) in breast carcinomas. (beds.ac.uk)
  • We examined FOXA1 in breast tumor and adjacent normal tissue in relation to reproductive factors, particularly higher parity and no breastfeeding, that are associated with ER − tumors. (aacrjournals.org)
  • Moreover, exposure of human umbilical vein endothelial cells to tumor necrosis factor-alpha resulted in marked down-regulation of GATA3 expression and reduction in Tie2 expression. (uniprot.org)
  • Diseases associated with GATA3 include Hypoparathyroidism, Sensorineural Deafness, And Renal Disease and End Stage Renal Disease . (genecards.org)
  • Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (provided by RefSeq, Nov 2009). (bio-rad-antibodies.com)
  • raises the question as to the role of Gata3 in mature sympathetic neurons and chromaffin cells. (jneurosci.org)
  • Additionally, mRNA analyses of purified chromaffin cells from Gata3 mutants show that levels of Mash1, Hand2 and Phox2b (postulated upstream regulators of Gata3) as well as terminally differentiated SA lineage products (tyrosine hydroxylase, Th, and dopamineβ -hydroxylase, Dbh) are markedly altered. (biologists.org)
  • In contrast, chromaffin cell survival depends on Gata3 only during embryonic development. (jneurosci.org)
  • The GATA3 transcription factor is critical for the embryonic development of various tissues as well as for inflammatory and humoral immune responses and the proper functioning of the endothelium of blood vessels. (wikipedia.org)
  • Gene-deletion studies in mice indicate that Gata3 (mouse gene equivalent to GATA3) is critical for the embryonic development and/or function of various cell types (e.g. fat cells, neural crest cells, lymphocytes) and tissues (e.g. kidney, liver, brain, spinal cord, mammary gland). (wikipedia.org)
  • [8] The Pax2 gene encodes for the transcription factor which appears to be essential in the organization of the midbrain and hindbrain regions, and at the earliest can be detected on either side of the sulcus limitans, which separates motor and sensory nerve nuclei. (wikipedia.org)
  • Analysis of GATA3 in the family revealed a heterozygous missense mutation resulting in a nonconservative change of a single amino acid (R276P) in the ZnF1 domain. (ox.ac.uk)
  • Clone REA174 recognizes GATA3, a member of the GATA family of transcription factors. (miltenyibiotec.com)
  • Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. (thermofisher.com)
  • 3) GATA3 promotes the secretion of IL-4, IL-5, and IL-13 from Th2 cells in humans and has similar actions on comparable mouse lymphocytes. (wikipedia.org)
  • Subsequently, miR-195-5p/NOTCH2 suppressed GATA3-mediated IL-4 secretion in CRC cells and ultimately inhibited M2-like TAM polarization. (springer.com)
  • GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Lysosomal Ca2+ Signaling Regulates High Glucose-Mediated Interleukin-1β Secretion via Transcription Factor EB in Human Monocytic Cells. (antibody-antibodies.com)
  • Lysosomal Ca2+ Signaling Regulates High Glucose-Mediated Interleukin-1β Secretion via Transcription Factor EB in Human Monocytic Cells. (antibody-antibodies.com)
  • Cross-talk between site-specific transcription factors and DNA methylation states J Biol Chem. (usc.edu)
  • NFIL3-deficient mice showed impaired development of NK cells and reduced expression of transcription factors ID2, EOMES, GATA3, and TBX21. (biolegend.com)
  • TCFL5 is a new bHLH transcription factor that negatively regulates upstream transcription factor-dependent transcription. (antibody-antibodies.com)
  • Function of GATA3 in ventral rhombomere (r) 4 is dependent on functional GATA2, which in turn is under the control of Hoxb1. (biologists.org)
  • Here, we determined the degree of functional overlap between the two transcription factor families in Arabidopsis ( Arabidopsis thaliana ), characterizing their ability to regulate chloroplast biogenesis both alone and in concert. (plantphysiol.org)
  • Chromatin immunoprecipitation and electrophoretic mobility-shift assays were employed to study the presence of a functional GATA3-binding site. (beds.ac.uk)
  • At a functional level, GATA3 knockdown inhibited angiopoietin (Ang)-1-mediated but not vascular endothelial cell growth factor (VEGF)-mediated AKT signaling, cell migration, survival, and tube formation. (uniprot.org)
  • GATA3 and MUC1 expression was analyzed in vitro with a GATA3 knockdown assay. (beds.ac.uk)
  • GATA3 knockdown blocked the ability of Ang-1 to attenuate vascular endothelial cell growth factor stimulation of vascular cell adhesion molecule-1 expression and monocytic cell adhesion. (uniprot.org)
  • Hematopoiesis is a carefully controlled process that is regulated by complex networks of transcription factors that are, in part, controlled by signals resulting from ligand binding to cell-surface receptors. (bloodjournal.org)
  • All three of these interleukins serve to promote allergic responses, 4) GATA3 induces the maturation of precursor cells into breast epithelial cells and maintains these cells in their mature state in mice and possibly humans. (wikipedia.org)
  • report that, in immune cells, TGF-β induces the Smad3 assocation with GATA-3, a transcription factor whose family has been implicated in regulating cell-fate decisions in the immune and nervous systems. (sciencemag.org)
  • In this study, we demonstrate that the ETS family transcription factor ETS variant (Etv)5 regulates IL-10 production in Th2 cells. (elsevier.com)
  • Trans-acting T-cell specific transcription factor GATA-3 is a member of GATA family of transcription factors that regulates development of multiple tissues. (antibody-antibodies.com)
  • The transcription factor GATA3 is essential for the genesis and maturation of the T cell lineage, and GATA3 dysregulation has pathological consequences. (jci.org)
  • GATA-3 is a trans-acting T-cell-specific transcription factor required for several developmental steps in T-cells [ PMID: 20969588 ]. (ebi.ac.uk)
  • As Gata3 expression is maintained up to the adult stage, we addressed its function in differentiated sympathoadrenal cells at embryonic and adult stages by conditional Gata3 elimination. (jneurosci.org)
  • The initial development of sympathoadrenal cells is regulated by a network of transcription factors, including Phox2b, Ascl1, Hand2, Insm1, and Gata2/3 ( Goridis and Rohrer, 2002 ). (jneurosci.org)
  • 2) In humans, GATA3 is required for the development and/or function of innate lymphoid cells (ILCs), particularly Group 2 ILCs as well as for the development of T helper cells,(Th cells), particularly Th2 cells. (wikipedia.org)
  • Group 2 ILCs and Th2 cells, and thereby GATA3, are critical for the development of allergic and humoral immune responses in humans. (wikipedia.org)
  • 5) In mice, GATA3 is responsible for the normal development of various tissues including the skin, fat cells, the thymus, and the nervous system. (wikipedia.org)
  • Expression of GATA3 is found in developing and mature T cells, natural killer (NK) cells, subset of NKT cells, and endothelial cells. (miltenyibiotec.com)
  • Transcription factor activity assay of GATA-3 from nuclear extracts of Jurkat cells or HeLa cells. (antibodies-online.com)
  • Transcription factor activity assay of GATA-3 from nuclear extracts of Jurkat cells or HeLa cells with the specific competitor or non-specific competitor. (antibodies-online.com)
  • Development of stem cell-based therapy requires a better understanding of factors and signals that drive stem cells into otic sensory progenitor cells (OSPCs) to replace lost HCs. (frontiersin.org)
  • We therefore designed the present study to elucidate whether MUC1 expression is regulated by GATA3 in breast cancer cells. (beds.ac.uk)
  • Previous studies have indicated that Th2 cells were able to differentiate into Th9 cells following the addition of transforming growth factor β (TGF-β) in the presence of IL-4 ( 7 ), and that Th9 cell polarization was further enhanced by IL-1, IL-2 and IL-25 ( 8 - 10 ). (spandidos-publications.com)
  • How these transcription factors work together during the development of CD4 and CD8 T cells, and how they are regulated by positive selection signals, is not yet clear. (rupress.org)
  • T1/ST2 is a surface ligand of the IL-1 receptor family, expressed on Th2- but not on interferon (IFN)-γ-producing Th1 cells. (pubmedcentralcanada.ca)
  • GCM1 (Glial Cells Missing factor 1) is an essential transcription factor for the expression of Syncytin-1 and is dependent on MAPK14 (also known as p38) phosphorylation [22, 23]. (immune-source.com)
  • Moreover, RNA sequencing demonstrated that rs4349147 G/− cells express a lower relative ratio of IL-32α to non-α isoforms than rs4349147 −/A cells and display increased expression of lymphocyte activation factors rendering them more prone to infection with HIV-1. (sciencemag.org)
  • The transcription factors that regulate IL-10 production in Th2 cells are still incompletely described. (elsevier.com)
  • Taken together, we conclude that Etv5 plays a crucial role in regulating IL-10 production in Th2 cells by facilitating the binding of IL-10-inducing transcription factors at the Il10 locus. (elsevier.com)
  • Here we provide evidence that the Ikaros family of DNA binding factors is critical for the activity of hemopoietic stem cells (HSCs) in the mouse. (rupress.org)
  • POU5F1 is a POU transcription factor expressed by early embryo cells and germ cells. (antibody-antibodies.com)
  • ERRs, which are coexpressed with ERs in prostatic cells, could regulate cell growth and modulate ER-mediated pathways via interference on ERalpha transcription in prostatic cells. (antibody-antibodies.com)
  • Immunofluorescent analysis of GATA3 (green) in MCF7 (left panel) and SH-SY5Y cells (right panel). (bio-rad-antibodies.com)
  • Cells were probed with GATA3 polyclonal antibody ( AHP2403 ) at a dilution of 1/50 for at least 1 hour at room temperature, washed with PBS, and incubated with a goat anti-rabbit IgG secondary antibody (green) for 30 minutes at room temperature. (bio-rad-antibodies.com)
  • Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells. (uniprot.org)
  • Together, these findings suggest that GATA3 is indispensable for Ang-1-Tie2-mediated signaling in large vessel endothelial cells. (uniprot.org)
  • Comparable studies in animals implicate GATA3 in the development of lymphocytes that mediate allergic and humoral immunity as well as allergic and humeral immune responses. (wikipedia.org)
  • Identification of the programed cell death 1 (PD-1) as another inhibitory receptor and inclusion of its ligands as additional members of the B7-1/B7-2-CD28/CTLA-4 family ( 14 , 15 ) re-established the importance of immune checkpoints to safeguard the maintenance of T cell tolerance. (frontiersin.org)
  • These SNPs were identified by in silico search to be located in transcription factor recognition sites which factors are presumably involved in the Th1 immune response and in the Th2 regulation pathway. (biomedcentral.com)
  • Several transcription factors that govern immune cell responses were detectable in the tertiary lymphoid follicles, among them Zap70, TFIIB, GATA3, and IRF4. (arvojournals.org)
  • Transfection experiments with dominant negative Trametinib mutants and constitutively Trametinib activated CREB expression vectors in addition to Chromatin Immunoprecipitation suggested that a CREB family member, CREB2 was binding and acting through the CRE/AP-1 motif. (immune-source.com)
  • Dr. Farnham is an international leader in the study of chromatin regulation and its control of transcription factor binding and function. (usc.edu)
  • Here we have examined the consequences of Gata3 deficiency on sympathetic neuron and adrenal medullary chromaffin cell development, anticipating possible revelation of new insights into Gata3 neuroendocrine function(s). (biologists.org)
  • and this significantly restores Th and Dbh deficiency in Gata3 mutant mice, thereby overcoming the Gata3 mutation-induced embryonic lethality. (biologists.org)
  • GATA3 has two trans-activating domains and two zinc finger domains, each followed by a conserved basic region. (miltenyibiotec.com)
  • GATA3 has 2 zinc finger domains, which play an important role in the increase in target gene transcription activity. (biomedcentral.com)
  • 3 4 In this context, GATA3, a transcription factor with two transactivating domains (TA1 and TA2) and two zinc finger domains (ZF1 and ZF2), 5 6 has been mapped to distal 10p, 7 and human GATA3 expression has been detected in the developing parathyroid glands, inner ears, and kidneys, together with the thymus and central nervous system (CNS). (bmj.com)
  • Among C2H2 ZNFs, there are a large number of transcription factors with the C- x -C- x -H- x -H motif, which mediates direct interaction with DNA. (nature.com)
  • These results thereby demonstrate that induced expression of Syncytin-2 is highly dependent on the interaction of bZIP-containing transcription factors to a CRE/AP-1 motif and that this element is important for the regulation of Syncytin-2 expression, which results Rabbit polyclonal to SERPINB5 in the formation of the peripheral syncytiotrophoblast layer. (immune-source.com)
  • The GATA3 gene is located close to the end of the short arm of chromosome 10 at position p14. (wikipedia.org)
  • Expression of GATA3 may be regulated in part or at times by the antisense RNA, GATA3-AS1, whose gene is located close to the GATA3 gene on the short arm of chromosome 10 at position p14. (wikipedia.org)
  • The location of GATA3 borders that of other critical sites on chromosome 10, particularly a site located at 10p14-p13. (wikipedia.org)
  • GATA3 belongs to the GATA family of transcription factors. (wikipedia.org)
  • NFIL3, also known as E4BP4, belongs to the basic leucine zipper (bZIP) family of transcription factors. (biolegend.com)
  • The basis for the term "interleukin" was to streamline the growing number of biological properties attributed to soluble factors from macrophages and lymphocytes. (wikipedia.org)
  • The lack of GATA3 expression in r4 inhibits the projection of contralateral vestibuloacoustic efferent neurons and the migration of facial branchiomotor neurons similar to Hoxb1-deficient mice. (biologists.org)
  • We identified GATA-3 as the most highly enriched transcription factor in the mammary epithelium of pubertal mice. (nih.gov)
  • This study highlights the importance of transcription factor ets-2 in the pathogenesis of pulmonary fibrosis in mice and humans through the regulation of Type I collagen expression. (pubmedcentralcanada.ca)
  • B. Transcription factor activity assay of GATA-3 from nuclear fractions with the GATA-3 Transcription Factor-Activity Assay Kit. (antibodies-online.com)
  • Two families of nuclear transcription factors, the GATA NITRATE-INDUCIBLE CARBON-METABOLISM-INVOLVED (GNC) and GOLDEN TWO-LIKE (GLK) families, have been implicated in directly and positively regulating chloroplast development. (plantphysiol.org)
  • Two families of nuclear transcription factors-the GATA NITRATE-INDUCIBLE CARBON-METABOLISM-INVOLVED (GNC) and GOLDEN TWO-LIKE (GLK) families-have been implicated in the direct, positive regulation of chloroplast biogenesis and thus are candidates for such master controllers. (plantphysiol.org)
  • Western blot analysis of GATA3 was performed by loading 15 ug of SH-SY5Y whole cell lysate (WCL), SH-SY5Y nuclear extract (NE) onto a 4-20% Tris-HCl polyacrylamide gel. (bio-rad-antibodies.com)
  • For example, both ATF and CREB matrices belong to the same bZIP family and recognise the TGACGT consensus sequence. (biomedcentral.com)
  • But this is not a common feature for all IL-1 family members, since IL-1β and IL-18 precursor forms do not bind their receptors and require proteolytic cleavage by either intracellular caspase-1 or extracellular neutrophilic proteases. (wikipedia.org)
  • In addition to cis-acting DNA sequences, and the transcription factors that bind them, information about mechanism of gene regulation can also come from analysis of the position of alleles within the nucleus ( 12 - 15 ). (rupress.org)