Extracellular Matrix
Extracellular Matrix Proteins
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Fibronectins
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
Collagen
Matrix Metalloproteinases
Matrix Metalloproteinase 2
Laminin
Tenascin
Hexameric extracellular matrix glycoprotein transiently expressed in many developing organs and often re-expressed in tumors. It is present in the central and peripheral nervous systems as well as in smooth muscle and tendons. (From Kreis & Vale, Guidebook to the Extracellular Matrix and Adhesion Proteins, 1993, p93)
Cells, Cultured
Matrix Metalloproteinase 1
Matrix Metalloproteinase Inhibitors
Integrins
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
Fibroblasts
Matrix Metalloproteinase 3
Bone Matrix
Nuclear Matrix
The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)
Cell Movement
Collagen Type I
Matrix Metalloproteinase 14
A transmembrane domain-containing matrix metalloproteinase. It is synthesized as an inactive zymogen that is activated by the action of PROPROTEIN CONVERTASES such as FURIN. Matrix metalloproteinase 14 plays a direct role in the cleavage of proteins in the pericellular environment. In addition, it can function indirectly by enzymatically activating the proprotein form of MATRIX METALLOPROTEINASE 15.
Tissue Inhibitor of Metalloproteinases
Hyaluronic Acid
Glycosaminoglycans
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Basement Membrane
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
Tissue Inhibitor of Metalloproteinase-1
Transforming Growth Factor beta
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
Antigens, CD29
Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)
Tissue Inhibitor of Metalloproteinase-2
A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a 21-kDa nonglycosylated protein found in tissue fluid and is secreted as a complex with progelatinase A by human fibroblast and uncomplexed from alveolar macrophages. An overexpression of TIMP-2 has been shown to inhibit invasive and metastatic activity of tumor cells and decrease tumor growth in vivo.
Immunohistochemistry
Collagen Type IV
A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.
Tissue Engineering
Matrix Metalloproteinase 13
Chondroitin Sulfate Proteoglycans
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Gelatinases
Matrix Metalloproteinase 7
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Cartilage
Versicans
Decorin
A small leucine-rich proteoglycan that interacts with FIBRILLAR COLLAGENS and modifies the EXTRACELLULAR MATRIX structure of CONNECTIVE TISSUE. Decorin has also been shown to play additional roles in the regulation of cellular responses to GROWTH FACTORS. The protein contains a single glycosaminoglycan chain and is similar in structure to BIGLYCAN.
Matrix Metalloproteinase 9
Antigens, CD147
Matrix Metalloproteinases, Membrane-Associated
Biglycan
Cell Differentiation
Tissue Scaffolds
Vitronectin
Fibrosis
Gelatin
Amino Acid Sequence
Thrombospondins
A family of related, adhesive glycoproteins which are synthesized, secreted, and incorporated into the extracellular matrix of a variety of cells, including alpha granules of platelets following thrombin activation and endothelial cells. They interact with a number of BLOOD COAGULATION FACTORS and anticoagulant factors. Five distinct forms have been identified, thrombospondin 1, -2, -3, -4, and cartilage oligomeric matrix protein (COMP). They are involved in cell adhesion, platelet aggregation, cell proliferation, angiogenesis, tumor metastasis, VASCULAR SMOOTH MUSCLE growth, and tissue repair.
Blotting, Western
Transforming Growth Factor beta1
A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Glycoproteins
Heparan Sulfate Proteoglycans
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
Cell Adhesion Molecules
Matrix Metalloproteinase 12
Stress, Mechanical
Protein Binding
Osteonectin
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Matrix Metalloproteinases, Secreted
Models, Biological
Aggrecans
Cattle
Cell Division
Fibrillar Collagens
Biocompatible Materials
Collagen Type III
Gene Expression
Epithelial Cells
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Heparitin Sulfate
Cytoskeleton
Hydrogels
Actins
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Calcification, Physiologic
Cartilage, Articular
Connective Tissue
Chick Embryo
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Receptors, Fibronectin
Specific cell surface receptors which bind to FIBRONECTINS. Studies have shown that these receptors function in certain types of adhesive contact as well as playing a major role in matrix assembly. These receptors include the traditional fibronectin receptor, also called INTEGRIN ALPHA5BETA1 and several other integrins.
Skin
Matrilin Proteins
PROTEOGLYCANS-associated proteins that are major components of EXTRACELLULAR MATRIX of various tissues including CARTILAGE; and INTERVERTEBRAL DISC structures. They bind COLLAGEN fibers and contain protein domains that enable oligomer formation and interaction with other extracellular matrix proteins such as CARTILAGE OLIGOMERIC MATRIX PROTEIN.
Matrix Metalloproteinase 10
Hyaluronoglucosaminidase
Cell Adhesion Molecules, Neuronal
Surface ligands that mediate cell-to-cell adhesion and function in the assembly and interconnection of the vertebrate nervous system. These molecules promote cell adhesion via a homophilic mechanism. These are not to be confused with NEURAL CELL ADHESION MOLECULES, now known to be expressed in a variety of tissues and cell types in addition to nervous tissue.
Collagen Type VI
Dystroglycans
Focal Adhesions
An anchoring junction of the cell to a non-cellular substrate. It is composed of a specialized area of the plasma membrane where bundles of the ACTIN CYTOSKELETON terminate and attach to the transmembrane linkers, INTEGRINS, which in turn attach through their extracellular domains to EXTRACELLULAR MATRIX PROTEINS.
Chondroitin Sulfates
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Cell-Matrix Junctions
Up-Regulation
Morphogenesis
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Base Sequence
Tumor Cells, Cultured
Phenotype
Latent TGF-beta Binding Proteins
Heparin
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
Mechanotransduction, Cellular
Microscopy, Fluorescence
Cartilage Oligomeric Matrix Protein
Tissue Inhibitor of Metalloproteinase-3
Focal Adhesion Protein-Tyrosine Kinases
Dermis
Disease Models, Animal
Connective Tissue Growth Factor
Rats, Sprague-Dawley
Cell Communication
Cell Surface Extensions
Protein-Lysine 6-Oxidase
Gels
Procollagen
Glomerular Mesangium
Endothelium, Vascular
Transfection
Gene Expression Profiling
Epithelium
Integrin alpha5beta1
Brevican
Urokinase-Type Plasminogen Activator
Protease Inhibitors
Matrix Metalloproteinase 11
A secreted matrix metalloproteinase that is believed to play a role in EXTRACELLULAR MATRIX remodeling and cell fate determination during normal and pathological processes. Matrix metalloproteinase 11 was originally isolated in primary BREAST NEOPLASMS and may be involved in the process of tumorigenesis.
Myofibroblasts
In Situ Hybridization
Microscopy, Confocal
Elastic Tissue
Collagen Type II
Electrophoresis, Polyacrylamide Gel
Culture Media, Conditioned
Elastic Modulus
Focal Adhesion Kinase 1
A non-receptor protein tyrosine kinase that is localized to FOCAL ADHESIONS and is a central component of integrin-mediated SIGNAL TRANSDUCTION PATHWAYS. Focal adhesion kinase 1 interacts with PAXILLIN and undergoes PHOSPHORYLATION in response to adhesion of cell surface integrins to the EXTRACELLULAR MATRIX. Phosphorylated p125FAK protein binds to a variety of SH2 DOMAIN and SH3 DOMAIN containing proteins and helps regulate CELL ADHESION and CELL MIGRATION.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Metalloproteases
Endothelial Cells
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Cell Survival
Stromal Cells
Biomimetic Materials
Keratan Sulfate
A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.
Osteoblasts
Antigens, CD44
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Integrin alpha5
Receptors, Laminin
Immunoblotting
Blotting, Northern
Drug Combinations
Receptors, Vitronectin
Gene Expression Regulation, Developmental
Growth Substances
Chondrogenesis
Neovascularization, Physiologic
Binding Sites
Oligonucleotide Array Sequence Analysis
Enzyme Activation
Mesoderm
Microfilament Proteins
Gene Expression Regulation, Enzymologic
Biomechanical Phenomena
Intercellular Signaling Peptides and Proteins
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
Cell Membrane
Viral Matrix Proteins
Membrane Proteins
Lung
Receptors, Cell Surface
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Trabecular Meshwork
Phosphorylation
Thrombospondin 1
An extracellular matrix glycoprotein from platelets and a variety of normal and transformed cells of both mesenchymal and epithelial origin. Thrombospondin-1 is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. Also, it has been studied for its use as a potential regulator of tumor growth and metastasis.
Mesenchymal Stromal Cells
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Plastics
Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Integrin alphaV
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
Cornea
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Peptide Fragments
Fluorescent Antibody Technique, Indirect
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins. (1/9343)
Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether Bcr-Abl assembles multi-protein complexes and if it does where these complexes are within cells. We have investigated the localization of Bcr-Abl in 32D myeloid cells attached to the extracellular matrix. We have found that Bcr-Abl displays a polarized distribution, colocalizing with a subset of filamentous actin at trailing portions of migrating 32D cells, and localizes on the cortical F-actin and on vesicle-like structures in resting 32D cells. Deletion of the actin binding domain of Bcr-Abl (Bcr-AbI-AD) dramatically enhances the localization of Bcr-Abl on the vesicle-like structures. These distinct localization patterns of Bcr-Abl and Bcr-Abl-AD enabled us to examine the localization of Bcr-Abl substrate and interacting proteins in relation to Bcr-Abl. We found that a subset of biochemically defined target proteins of Bcr-Abl redistributed and co-localized with Bcr-Abl on F-actin and on vesicle-like structures. The co-localization of signaling proteins with Bcr-Abl at its sites of localization supports the idea that Bcr-Abl forms a multi-protein signaling complex, while the polarized distribution and vesicle-like localization of Bcr-Abl may play a role in leukemogenesis. (+info)Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2. (2/9343)
Interaction between integrin alphavbeta3 and extracellular matrix is crucial for endothelial cells sprouting from capillaries and for angiogenesis. Furthermore, integrin-mediated outside-in signals co-operate with growth factor receptors to promote cell proliferation and motility. To determine a potential regulation of angiogenic inducer receptors by the integrin system, we investigated the interaction between alphavbeta3 integrin and tyrosine kinase vascular endothelial growth factor receptor-2 (VEGFR-2) in human endothelial cells. We report that tyrosine-phosphorylated VEGFR-2 co-immunoprecipitated with beta3 integrin subunit, but not with beta1 or beta5, from cells stimulated with VEGF-A165. VEGFR-2 phosphorylation and mitogenicity induced by VEGF-A165 were enhanced in cells plated on the alphavbeta3 ligand, vitronectin, compared with cells plated on the alpha5beta1 ligand, fibronectin or the alpha2beta1 ligand, collagen. BV4 anti-beta3 integrin mAb, which does not interfere with endothelial cell adhesion to vitronectin, reduced (i) the tyrosine phosphorylation of VEGFR-2; (ii) the activation of downstream transductor phosphoinositide 3-OH kinase; and (iii) biological effects triggered by VEGF-A165. These results indicate a new role for alphavbeta3 integrin in the activation of an in vitro angiogenic program in endothelial cells. Besides being the most important survival system for nascent vessels by regulating cell adhesion to matrix, alphavbeta3 integrin participates in the full activation of VEGFR-2 triggered by VEGF-A, which is an important angiogenic inducer in tumors, inflammation and tissue regeneration. (+info)Inhibition of transforming growth factor beta production by nitric oxide-treated chondrocytes: implications for matrix synthesis. (3/9343)
OBJECTIVE: Nitric oxide (NO) is generated copiously by articular chondrocytes activated by interleukin-1beta (IL-1beta). If NO production is blocked, much of the IL-1beta inhibition of proteoglycan synthesis is prevented. We tested the hypothesis that this inhibitory effect of NO on proteoglycan synthesis is secondary to changes in chondrocyte transforming growth factor beta (TGFbeta). METHODS: Monolayer, primary cultures of lapine articular chondrocytes and cartilage slices were studied. NO production was determined as nitrite accumulation in the medium. TGFbeta bioactivity in chondrocyte- and cartilage-conditioned medium (CM) was measured with the mink lung epithelial cell bioassay. Proteoglycan synthesis was measured as the incorporation of 35S-sodium sulfate into macromolecules separated from unincorporated label by gel filtration on PD-10 columns. RESULTS: IL-1beta increased active TGFbeta in chondrocyte CM by 12 hours; by 24 hours, significant increases in both active and latent TGFbeta were detectable. NG-monomethyl-L-arginine (L-NMA) potentiated the increase in total TGFbeta without affecting the early TGFbeta activation. IL-1beta stimulated a NO-independent, transient increase in TGFbeta3 at 24 hours; however, TGFbeta1 was not changed. When NO synthesis was inhibited with L-NMA, IL-1beta increased CM concentrations of TGFbeta1 from 24-72 hours of culture. L-arginine (10 mM) reversed the inhibitory effect of L-NMA on NO production and blocked the increases in TGFbeta1. Anti-TGFbeta1 antibody prevented the restoration of proteoglycan synthesis by chondrocytes exposed to IL-1beta + L-NMA, confirming that NO inhibition of TGFbeta1 in IL-1beta-treated chondrocytes effected, in part, the decreased proteoglycan synthesis. Furthermore, the increase in TGFbeta and proteoglycan synthesis seen with L-NMA was reversed by the NO donor S-nitroso-N-acetylpenicillamide. Similar results were seen with cartilage slices in organ culture. The autocrine increase in CM TGFbeta1 levels following prior exposure to TGFbeta1 was also blocked by NO. CONCLUSION: NO can modulate proteoglycan synthesis indirectly by decreasing the production of TGFbeta1 by chondrocytes exposed to IL-1beta. It prevents autocrine-stimulated increases in TGFbeta1, thus potentially diminishing the anabolic effects of this cytokine in chondrocytes. (+info)Role of thrombin receptor in breast cancer invasiveness. (4/9343)
Invasion, the ability of an epithelial cancer cell to detach from and move through a basement membrane, is a central process in tumour metastasis. Two components of invasion are proteolysis of extracellular matrix and cellular movement through it. A potential promoter of these two processes is thrombin, the serine proteinase derived from the ubiquitous plasma protein prothrombin. Thrombin promotes the invasion of MDA-MB231 breast tumour cells (a highly aggressive cell line) in an in vitro assay. Invasion by MDA-MB436 and MCF-7 cells, less aggressive cell lines, is not promoted by thrombin. Thrombin, added to the cells, is a stimulator of cellular movement; fibroblast-conditioned medium is the chemotaxin. Thrombin-promoted invasion is inhibited by hirudin. Stimulation of invasion is a receptor-mediated process that is mimicked by a thrombin receptor-activating peptide. Thrombin has no effect on chemotaxis in vitro. Thrombin receptor is detectable on the surface of MDA-MB231 cells, but not on the other two cell lines. Introduction of oestrogen receptors into MDA-MB231 cells by transfection with pHEO had no effect on thrombin receptor expression, in the presence or absence of oestradiol. This paper demonstrates that thrombin increases invasion by the aggressive breast cancer cell line MDA-MB231 by a thrombin receptor-dependent mechanism. (+info)Extracellular matrix remodelling in the endometrium and its possible relevance to the pathogenesis of endometriosis. (5/9343)
Essential features of endometrial physiology involve the extracellular matrix (ECM). In the pathogenesis of endometriosis, interactions of endometriosis cells with ECM can be postulated. Two systems of secreted proteases in the endometrium, the plasmin(ogen) activator/inhibitor and the matrix metalloproteinases and their inhibitors were examined in cell cultures of uterine endometrial cells from women with and without endometriosis. Soluble urokinase receptor secretion is increased, and mRNA transcription of tissue inhibitor of metalloproteinases-2 (TIMP-2) is upregulated by progestin in endometriosis. These findings are compatible with an altered ECM turnover in the endometrium of these patients that may explain a higher invasive potential of retrogradely menstruated endometrial fragments. (+info)Mechanisms and mediators in coal dust induced toxicity: a review. (6/9343)
Chronic inhalation of coal dust can cause several lung disorders, including simple coal workers pneumoconiosis (CWP), progressive massive fibrosis (PMF), chronic bronchitis, lung function loss, and emphysema. This review focuses on the cellular actions and interactions of key inflammatory cells and target cells in coal dust toxicity and related lung disorders, i.e. macrophages and neutrophils, epithelial cells, and fibroblasts. Factors released from or affecting these cells are outlined in separate sections, i.e. (1) reactive oxygen species (ROS) and related antioxidant protection mechanisms, and (2) cytokines, growth factors and related proteins. Furthermore, (3) components of the extracellular matrix (ECM), including the modifying role of ROS, cytokines, proteases and antiproteases are discussed in relation to tissue damage and remodelling in the respiratory tract. It is recognised that inhaled coal dust particles are important non-cellular and cellular sources of ROS in the lung, and may be significantly involved in the damage of lung target cells as well as important macromolecules including alpha-1-antitrypsin and DNA. In vitro and in vivo studies with coal dusts showed the up-regulation of important leukocyte recruiting factors, e.g. Leukotriene-B4 (LTB4), Platelet Derived Growth Factor (PDGF), Monocyte Chemotactic Protein-1 (MCP-1), and Tumor Necrosis Factor-alpha (TNF alpha), as well as the neutrophil adhesion factor Intercellular Adhesion Molecule-1 (ICAM-1). Coal dust particles are also known to stimulate the (macrophage) production of various factors with potential capacity to modulate lung cells and/or extracellular matrix, including O2-., H2O2, and NO, fibroblast chemoattractants (e.g. Transforming Growth Factor-beta (TGF beta), PDGF, and fibronectin) and a number of factors that have been shown to stimulate and/or inhibit fibroblast growth or collagen production such as (TNF alpha, TGF beta, PDGF, Insulin Like Growth Factor, and Prostaglandin-E2). Further studies are needed to clarify the in vivo kinetics and relative impact of these factors. (+info)Matrix valency regulates integrin-mediated lymphoid adhesion via Syk kinase. (7/9343)
Lymphocytes accumulate within the extracellular matrix (ECM) of tumor, wound, or inflammatory tissues. These tissues are largely comprised of polymerized adhesion proteins such as fibrin and fibronectin or their fragments. Nonactivated lymphoid cells attach preferentially to polymerized ECM proteins yet are unable to attach to monomeric forms or fragments of these proteins without previous activation. This adhesion event depends on the appropriate spacing of integrin adhesion sites. Adhesion of nonactivated lymphoid cells to polymeric ECM components results in activation of the antigen receptor-associated Syk kinase that accumulates in adhesion-promoting podosomes. In fact, activation of Syk by antigen or agonists, as well as expression of an activated Syk mutant in lymphoid cells, facilitates their adhesion to monomeric ECM proteins or their fragments. These results reveal a cooperative interaction between signals emanating from integrins and antigen receptors that can serve to regulate stable lymphoid cell adhesion and retention within a remodeling ECM. (+info)alphaSU2, an epithelial integrin that binds laminin in the sea urchin embryo. (8/9343)
At gastrulation in the sea urchin embryo dramatic cell adhesion changes contribute to primary mesenchyme cell ingression movements and to cell rearrangements during archenteron invagination. At ingression, quantitative adhesion assays demonstrated previously that primary mesenchyme cells (PMCs) change their affinity for neighboring cells, for a fibronectin-like substrate, and for the hyaline layer. To investigate the molecular basis for these and other differential cell affinities at gastrulation, we have identified an integrin that appears to be responsible for specific alterations in cell-substrate adhesion to laminin. During early cleavage stages blastomeres adhere poorly to laminin substrates. Around hatching there is a large increase in the ability of blastomeres to bind to laminin and this increase correlates temporally with the expression of an integrin on the basal surface all blastomeres. PMCs, after undergoing their epithelial-mesenchymal transition, have a strongly reduced affinity for laminin relative to ectoderm cells and, correspondingly, do not stain for the presence of the integrin. We identified the alpha integrin cDNA from Lytechinus variegatus by RT-PCR. Overlapping clones were obtained from a midgastrula cDNA library to provide a complete sequence for the integrin. The composite cDNA encoded a protein that was most similar to the alpha5 subgroup of vertebrate integrins, but there was not a definitive vertebrate integrin homolog. Northern blots and Western immunoblots showed that the sea urchin integrin, which we have named alphaSU2, is present in eggs and during all stages of development. Immunolocalization with specific polyclonal antibodies showed that alphaSU2 first appears on the basal cell surface of epithelia at the midblastula stage, at a time correlating with the increase in adhesive affinity for laminin. The protein remains at high levels on the basal surface of ectoderm cells but is temporarily reduced or eliminated from endoderm cells during their convergent-extension movements. To confirm integrin binding specificity, alphaSU2 was transfected into an alpha-integrin-deficient CHO cell line. alphaSU2-expressing CHO cells bound well to isolated sea urchin basal lamina and to purified laminin. The transfected cells bound weakly or not at all to fibronectin, type I collagen, and type IV collagen. This is consistent with the hypothesis that alphaSU2 integrin functions by binding epithelial cells to laminin in the basal lamina. In vivo, modulation of alphaSU2 integrin expression correlates with critical adhesive changes during cleavage and gastrulation. Thus, this protein appears to be an important contributor to the morphogenetic rearrangements that characterize gastrulation in the sea urchin embryo. (+info)
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Extra-cellular matrix proteins induce matrix metalloproteinase-1 (MMP-1) activity and increase airway smooth muscle contraction...
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Mechanobiology of myofibroblast adhesion in fibrotic cardiac disease | Journal of Cell Science
Cells Expressing CD44 Antigen (CDw44 or Epican or Extracellular Matrix Receptor III or GP90 Lymphocyte Homing/Adhesion Receptor...
extracellular matrix of blood
Distribution volumes of macromolecules in human ovarian and endometrial cancers--effects of extracellular matrix structure. |...
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MicroRNA-196a links human body fat distribution to adipose tissue extracellular matrix composition - Wellcome Centre for Human...
Changes in vascular extracellular matrix composition during decidual spiral arteriole remodeling in early human pregnancy. |...
Comparison the Prognostic Value of Galectin-3 and Serum Markers of Cardiac Extracellular Matrix Turnover in Patients with...
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Modulation of extracellular matrix genes reflects the magnitude of physiological adaptation to aerobic exercise training in...
Cancer Cell Migration: Integrated Roles of Matrix Mechanics and Transforming Potential
Group Seminar: Roeland Merks (Various), Stigmergy in blood vessel growth: how indirect mechanical and chemical signaling, via...
Download Extracellular Matrix Influences on Gene Expression by Harold C. Slavkin, Harold Slavkin PDF - COLORADOLIVING365.COM...
extracellular matrix function quizlet
Volume 2.35 | Sep 8 - Extracellular Matrix News
Patent US20080140094 - Unitary surgical device and method - Google Patentsuche
Dual-site recognition of different extracellular matrix components by anti-angiogenic/neurotrophic serpin, PEDF - Fingerprint
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The ExtraCellular Matrix (of Your Vagina) - Womens Health Practice
Extracellular matrix synonyms, Extracellular matrix antonyms - FreeThesaurus.com
Disputation: Karolina Minta - Fluid biomarkers of extracellular matrix remodelling across neurological diseases | University of...
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Free-radical crosslinking of specific proteins alters the function of the egg extracellular matrix at fertilization |...
Gene Ontology Classifications
The relationship between myocardial extracellular matrix remodeling and ventricular function
Regulation of the extracellular matrix by microRNA: impact on cancer metastases - QIAGEN
Patent US6790220 - Method and apparatus for sealing access - Google Patents
RePub, Erasmus University Repository:
Integrated genomics of chemotherapy resistant ovarian cancer: a role for extracellular...
Juxtacrine signalling
Cell / extracellular matrix signaling[edit]. The extracellular matrix is composed of glycoproteins (proteins and ... In biology, juxtacrine signalling (or contact-dependent signalling) is a type of cell / cell or cell / extracellular matrix ... An extracellular matrix glycoprotein and a membrane protein interact.. Additionally, in unicellular organisms such as bacteria ... Cell adhesion, mechanical adhesion between cells and/or the extracellular matrix. *Role of cell adhesions in neural development ...
Type IV collagen
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Calsequestrin
Extracellular matrix. *Matrix gla protein. This article incorporates text from the public domain Pfam and InterPro IPR001393 ...
Elastin
extracellular region. • elastic fiber. • extracellular matrix. • collagen-containing extracellular matrix. Biological process. ... extracellular matrix structural constituent. • protein binding. Cellular component. • proteinaceous extracellular matrix. • ... extracellular matrix disassembly. • extracellular matrix organization. • blood circulation. • respiratory gaseous exchange. ... into elastin fibres immediately after their synthesis by the cell and during their export into the extracellular matrix. ...
Scleroprotein
The roles of such proteins include protection and support, forming connective tissue, tendons, bone matrices, and muscle fiber ... Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Procollagen peptidase
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Laminin, alpha 4
extracellular exosome. • basal lamina. • extracellular matrix. • collagen-containing extracellular matrix. Biological process. ... extracellular matrix structural constituent. • protein binding. Cellular component. • proteinaceous extracellular matrix. • ... extracellular matrix organization. • regulation of cell migration. • regulation of embryonic development. • negative regulation ... Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They ...
Connective tissue
extracellular matrix Reticular fibers. Form a scaffolding for other cells. Type III collagen. liver, bone marrow, and lymphatic ... Both the ground substance and proteins (fibers) create the matrix for CT. Connective tissues are derived from the mesenchyme. ... Cells are spread through an extracellular fluid.. *Ground substance - A clear, colorless, and viscous fluid containing ...
Collagen, type XX, alpha 1
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
A. Hari Reddi
Extracellular Matrix Biochemistry. Elsevier, New York, 1984. Reddi A.H. (Ed.), Extracellular Matrix: Structure and Function. A. ... Homology of bone-inductive proteins from human, monkey, bovine, and rat extracellular matrix. Proc Natl Acad Sci U S A 1983; 80 ... The Reddi laboratory has also made important discoveries unraveling the role of the extracellular matrix in bone and cartilage ... They demonstrated first that BMPs bind the extracellular matrix, are present at the apical ectodermal ridge in the developing ...
CD248 - Википедия
extracellular matrix binding. Компонент клетки. • proteinaceous extracellular matrix. • cytoplasm. • integral component of ... Tomkowicz B., Rybinski K., Foley B., et al. Interaction of endosialin/TEM1 with extracellular matrix proteins mediates cell ... extracellular vesicular exosome. Биологический процесс. • biological_process. • positive regulation of cell proliferation. • ...
Interstitium
Extracellular matrix Bert JL; Pearce RH (1984). The interstitium and microvascular exchange. In: Handbook of Physiology. The ... The interstitial compartment is composed of connective and supporting tissues within the body - called the extracellular matrix ...
Laminin
Kühn, Klaus (1997). "Extracellular matrix constituents as integrin ligands". In Elbe, Johannes A. (ed.). Integrin-ligand ... Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. They are a major component of the ... The trimeric proteins intersect to form a cross-like structure that can bind to other cell membrane and extracellular matrix ... M. A. Haralson; John R. Hassell (1995). Extracellular matrix: a practical approach. Ithaca, N.Y: IRL Press. ISBN 978-0-19- ...
Tenascin
... s are extracellular matrix glycoproteins. They are abundant in the extracellular matrix of developing vertebrate ... One mechanism to explain this may come from its ability to bind to the extracellular matrix glycoprotein fibronectin and block ...
EMI domain
... extracellular matrix glycoproteins. Vertebrate Emu proteins, which could interact with several different extracellular matrix ... Proteins known to contain an EMI domain include: Vertebrate Emilins, extracellular matrix glycoproteins. Vertebrate Multimerins ... The EMI domain is most often found at the N terminus of metazoan extracellular proteins that are forming or are compatible with ... a novel cysteine-rich domain of EMILINs and other extracellular proteins, interacts with the gC1q domains and participates in ...
Chondroitinase treatment
Plasticity and the extracellular matrix. Archived 2007-12-03 at the Wayback Machine Hensch TK (2005). "Critical period ...
Cell and Tissue Research
... extracellular Extracellular matrix; D. Furst, muscle; Joseph Bonventre, kidney and related subjects; P. Sutovsky, reproductive ...
Arterial stiffness
Dietz, J (2007). "Arterial stiffness and extracellular matrix". Atherosclerosis, Large Arteries and Cardiovascular Risk. Adv. ...
Mesoglea
Anatomy of corals Sarras, M. P.; Madden, M. E.; Zhang, X.; Gunwar, S.; Huff, J. K.; Hudson, B. G. (1991). "Extracellular matrix ... a more-or-less solid but loosely organized tissue consisting of a gel matrix [the mesoglea, in strict sense] with various ...
Generative tissue
Extracellular Matrix Biomaterials for Soft Tissue Repair. Clin Podiatr Med Surg 26 (2009) 507-523 Article Valentin JE, Badylak ... gTissue is a type of living Tissue (biology), hence an ensemble of cells and extracellular matrix that carry out a particular ... Extracellular matrix bioscaffolds for orthopaedic applications. A comparative histologic study. J Bone Joint Surg Am 2006;88(12 ... For example, during tendon augmentation procedures, the collagen fiber architecture of the extracellular matrix can transition ...
Keith Burridge
"Signal transduction from the extracellular matrix". The Journal of Cell Biology. 120 (3): 577-85. doi:10.1083/jcb.120.3.577. ... Actin-adhesion links to the extracellular matrix". Nature Milestones. doi:10.1038/nrm2568. Burridge K, Feramisco JR; Feramisco ... "Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal ...
Cell wall
An extracellular matrix (ECM) is also present in metazoans. Its composition varies between cells, but collagens are the most ... Extracellular matrix Bacterial cell structure Plant cell Romaniuk JA, Cegelski L (October 2015). "Bacterial cell wall ... Frantz C, Stewart KM, Weaver VM (December 2010). "The extracellular matrix at a glance". Journal of Cell Science. 123 (Pt 24): ... Roberts K (October 1994). "The plant extracellular matrix: in a new expansive mood". Current Opinion in Cell Biology. 6 (5): ...
Mesangial cell
Accumulation of extracellular matrix proteins then occurs due to insufficient degradation by matrix metalloproteinases. ... Mesangial cells are separated by intercellular spaces containing extracellular matrix called the mesangial matrix that is ... Mesangial matrix provides structural support for the mesangium. Mesangial matrix is composed of glomerular matrix proteins such ... Mason, R; Wahab, N (2003). "Extracellular Matrix Metabolism in Diabetic Nephropathy". Journal of the American Society of ...
3D cell culture in wood-based nanocellulose hydrogel
As the natural extracellular matrix (ECM) is important in the survival, proliferation, differentiation and migration of the ... Tibbitt, Mark W.; Anseth, Kristi S. (2009). "Hydrogels as extracellular matrix mimics for 3D cell culture". Biotechnology and ... Geckil, Hikmet; Xu, Feng; Zhang, Xiaohui; Moon, SangJun; Demirci, Utkan (2010). "Engineering hydrogels as extracellular matrix ... Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture. As plant based material, it ...
Collagen, type I, alpha 1
Bou-Gharios G, Ponticos M, Rajkumar V, Abraham D (2004). "Extra-cellular matrix in vascular networks". Cell Prolif. 37 (3): 207 ...
Nanofiber
... scaffolds are used in bone tissue engineering to mimic the natural extracellular matrix of the bones. The bone tissue ... Mo XM, Xu CY, Kotaki M, Ramakrishna S (May 2004). "Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for ... In tissue engineering, a highly porous artificial extracellular matrix is needed to support and guide cell growth and tissue ... An electrospun nanofiber network resembles the extracellular matrix (ECM) well. This resemblance is a major advantage of ...
PAPLN
Papilin is an extracellular matrix glycoprotein. GRCh38: Ensembl release 89: ENSG00000100767 - Ensembl, May 2017 GRCm38: ...
Cenderitide
Weber KT, Swamynathan SK, Guntaka RV, Sun Y (1999). "Angiotensin II and extracellular matrix homeostasis". The International ... Marked upregulation of genes encoding extracellular matrix components". Circulation Research. 75 (1): 23-32. doi:10.1161/01.res ... compensate with a number of structural alterations including hypertrophy of cardiomyocytes and increase of extracellular matrix ...
McGill University Faculty of Dentistry
... mineralized tissues and extracellular matrix biology; biomaterials, nanobiotechnology and tissue engineering; and population ...
3D cell culture
This work focused on the importance of the extracellular matrix and the ability of cultures in artificial 3D matrices to ... As the natural extracellular matrix (ECM) is important in the survival, proliferation, differentiation and migration of the ... Geckil H, Xu F, Zhang X, Moon S, Demirci U (April 2010). "Engineering hydrogels as extracellular matrix mimics". Nanomedicine. ... Prestwich GD (August 2007). "Simplifying the extracellular matrix for 3-D cell culture and tissue engineering: a pragmatic ...
Food web
... extra-cellular polymers, nectar, root exudates and leachates, dissolved organic matter, extra-cellular matrix, mucilage). The ...
Catenin
In particular, abnormal interactions between epithelial cells and the extracellular matrix are associated with the over- ...
Elastina - Wikipédia, a enciclopédia livre
extracellular matrix structural constituent. •protein binding. •extracellular matrix constituent conferring elasticity. • ... proteinaceous extracellular matrix. •mitochondrion. Processo biológico. •blood vessel remodeling. •skeletal muscle tissue ... extracellular matrix organization. •regulation of actin filament polymerization. •stress fiber assembly. Sources: Amigo / ...
ZP3
GO:0005578 extracellular matrix. • plasma membrane. • extracellular region. • extracellular space. • intracellular anatomical ... extracellular matrix structural constituent. • structural constituent of egg coat. Cellular component. • integral component of ... collagen-containing extracellular matrix. Biological process. • positive regulation of inflammatory response. • positive ... The zona pellucida (ZP) is a specialized extracellular matrix that surrounds the oocyte and early embryo. It is composed of ...
Asporin - ويكيبيديا
collagen-containing extracellular matrix. العمليات الحيوية. • bone mineralization. • biomineral tissue development. • negative ...
Kluyveromyces marxianus
... are often resistant to commonly used antifungal agents because of difficulty in penetrating the extracellular polymeric matrix. ...
Lyme disease
Borrelia burgdorferi and the extracellular matrix". Trends in Microbiology. 15 (8): 350-4. doi:10.1016/j.tim.2007.06.003. PMID ... and hiding in the extracellular matrix, which may interfere with the function of immune factors.[89][90] ...
Connective tissue
extracellular matrix Reticular fibers. Form a scaffolding for other cells. Type III collagen. liver, bone marrow, and lymphatic ... Both the ground substance and proteins (fibers) create the matrix for CT. Connective tissues are derived from the mesenchyme. ... Cells are spread through an extracellular fluid.. *Ground substance - A clear, colorless, and viscous fluid containing ...
Amelogenin
They are a type of extracellular matrix (ECM) protein, which, together with ameloblastins, enamelins, and tuftelins direct the ... mineralization of enamel to form a highly organized matrix of rods, interrod crystal, and protein. Although the precise role of ...
Competitive inhibition
MPP+ eventually travels to the extracellular fluid by a dopamine transporter, which ultimately causes the Parkinson's symptoms ...
Peroxisome
... s can be derived from the endoplasmic reticulum and replicate by fission.[13] Peroxisome matrix proteins are ... of peroxisomal matrix proteins signals them to be imported into the organelle. There are at least 32 known peroxisomal proteins ... is translocated into the peroxisomal matrix and recycled to the cytosol". Cell. 105 (2): 187-96. doi:10.1016/s0092-8674(01) ... and may occur without the import of the matrix (lumen) enzymes. Proliferation of the organelle is regulated by Pex11p. ...
Hunter syndrome
One of the parts of the extracellular matrix is a complex molecule called a proteoglycan. Like many components of the body, ... The matrix surrounds the cells of the body in an organized meshwork and functions as the glue that holds the cells of the body ... This matrix is made up of a variety of sugars and proteins and helps to form the architectural framework of the body. ... of Hunter syndrome is related to a problem in a part of the connective tissue of the body known as the extracellular matrix. ...
Brain-derived neurotrophic factor
"PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix-dependent phosphatidylinositol 3- ... extracellular region. • extracellular. • synaptic vesicle. • axon. • dendrite. Biological process. • brain-derived neurotrophic ...
Dentin
Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis For Repair and Tissue Engineering, M. Goldberg and A.J ... Dentin is a bone-like matrix that is porous and yellow-hued material. It is made up of 70% inorganic materials (mainly ... The innermost layer of dentin is known as predentin, and is the initial dentin matrix that is laid down prior to mineralisation ... Matrix metalloproteinases (MMPs) and their specific tissue inhibitors (TIMPs) in mature human odontoblasts and pulp tissue. ...
Olfactory ensheathing cells
... sequences so the transplantation cells have closely related properties to that of native tissue in the extracellular matrix.[3] ... the inability of a sufficient matrix to thrive and create a uniform population of cells, or the migratory response of the cells ... by the body and biological functions such as cell adhesion and growth will be enhanced through cell-cell and cell-matrix ...
Spatial memory
Beginning with a small 2 x 2 matrix, participants copy the matrix pattern from memory into an empty matrix. The matrix patterns ... Hebert, A. E.; Dash, P. K. (2002). "Extracellular signal-regulated kinase activity in the entorhinal cortex is necessary for ... The matrix patterns are arranged in a way that is difficult to code verbally, forcing the participant to rely on visual spatial ... At the end, the participant is asked to indicate on a real matrix where the little man that he or she visualized finished. The ...
FLNB
extracellular matrix. • cytosol. • cell nucleus. • neuron projection. • neuronal cell body. Biological process. • muscle organ ... extracellular exosome. • stress fiber. • plasma membrane. • Z disc. • cytoplasm. • cell cortex. • focal adhesion. • ...
EMILIN1
proteinaceous extracellular matrix. • extracellular region. • extracellular exosome. • extracellular space. • extracellular ... extracellular matrix constituent conferring elasticity. • identical protein binding. • integrin binding involved in cell-matrix ... It is the best characterized member of the EMILIN family of extracellular matrix glycoproteins. ... matrix. • integrin alpha4-beta1 complex. • EMILIN complex. Biological process. • cell adhesion. • cell-matrix adhesion. • cell ...
Autophagy
Extracellular matrix *Cell wall. Retrieved from "https://en.wikipedia.org/w/index.php?title=Autophagy&oldid=821997179" ...
Glossary of biology
Contrast extracellular.. introduced species. Also called an exotic species, foreign species, alien species, non-native species ... In eukaryotes, the cytoskeletal matrix is a dynamic structure composed of three main proteins, which are capable of rapid ... extracellular. Of or occurring in the space outside the plasma membrane of a cell. Contrast intracellular.. extranuclear ... The minus sign indicates that the inside of the cell is negative with respect to the surrounding extracellular fluid.. ...
S100A6 - ويكيبيديا
extracellular exosome. • نواة. • منطقة خارج الخلية. • collagen-containing extracellular matrix. العمليات الحيوية. • ...
Platelet-derived growth factor
... accelerating extracellular matrix and collagen formation and thus reducing the time for the healing process to occur.[22] ... "The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor". Biomaterials. 35 ...
Hyaluronidase
The majority of mammalian ova are covered in a layer of granulosa cells intertwined in an extracellular matrix that contains a ... By catalyzing the hydrolysis of hyaluronan, a constituent of the extracellular matrix (ECM), hyaluronidase lowers the viscosity ... "Matrix Biology. 20 (8): 499-508. doi:10.1016/S0945-053X(01)00172-X. PMID 11731267.. ... although exogenous hyaluronidases can disrupt the cumulus matrix. ...
Tumor necrosis factor alpha
extracellular matrix organization. • positive regulation of sequence-specific DNA binding transcription factor activity. • ... extracellular region. • plasma membrane. • membrane raft. • extracellular space. Biological process. • regulation of protein ...
Autophagy
Extracellular matrix. Retrieved from "https://en.wikipedia.org/w/index.php?title=Autophagy&oldid=869052817" ...
Zelula kanpoko matrize, entziklopedia askea.
Ingelesez) «Extracellular matrix structure» Advanced Drug Delivery Reviews (97): 4-27 2016-02-01 doi:10.1016/j.addr.2015.11.001 ... Ingelesez) Mecham, Robert (2011-02-16) The Extracellular Matrix: an Overview Springer Science & Business Media ISBN ... Yue, Beatrice (2014) «Biology of the Extracellular Matrix: An Overview» Journal of glaucoma: S20-S23 doi:10.1097/IJG. ... The Extracellular Matrix of Animals» Molecular Biology of the Cell. 4th edition . Noiz kontsultatua: 2018-11-22 . ...
Medical microbiology
Additionally, the extracellular matrix and dense outer layer of bacterial cells can protect the inner bacteria cells from ... biofilms by adhering to surfaces on implanted devices such as catheters and prostheses and creating an extracellular matrix for ...
Influenza
... matrix 1 protein), M2, NS1 (non-structural protein 1), NS2 (other name is NEP, nuclear export protein), PA, PB1 (polymerase ... "A universal influenza A vaccine based on the extracellular domain of the M2 protein". Nat. Med. 5 (10): 1157-63. doi:10.1038/ ...
Insulin-like growth factor 2
extracellular region. • platelet alpha granule lumen. • extracellular. Biological process. • regulation of gene expression by ... and Matrix Attachment Region −3 (MAR3). These three DNA sequences bind to CTCF in a way that limits downstream enhancer access ...
Extracellular matrix - Wikipedia
Extracellular matrix. Illustration depicting extracellular matrix (basement membrane and interstitial matrix) in relation to ... The animal extracellular matrix includes the interstitial matrix and the basement membrane.[5] Interstitial matrix is present ... Extracellular matrix: review of its roles in acute and chronic wounds. *Usage of Extracellular Matrix from pigs to regrow human ... In biology, the extracellular matrix (ECM) is a three-dimensional network of extracellular macromolecules, such as collagen, ...
Category:Extracellular matrix proteins - Wikimedia Commons
Media in category "Extracellular matrix proteins". The following 85 files are in this category, out of 85 total. ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s007.ogv 15 s, ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s008.ogv 15 s, ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s009.ogv 15 s, ...
Matrigel Matrix | Extracellular Matrix | Corning
Matrigel Matrix , Extracellular Matrix , Corning. We use cookies to ensure the best experience on our website. ... Cells behave better on Corning Matrigel matrix-the original, trusted extracellular matrix (ECM).. Nearly 30 years ago, ... Corning Matrigel Matrix FAQs Corning Matrigel Matrix FAQs Get answers to commonly asked questions about Corning Matrigel matrix ... Extracellular matrices are complex biological reagents, and, like all biologically derived reagents, they may be subject to lot ...
Extracellular Matrices
Evolution of Extracellular Matrix | Fred W. Keeley | Springer
... by the extracellular matrix. The proteins and glycoconjugates that make up the extracellular matrix provide structural support ... Biology of Extracellular Matrix. Copyright. 2013. Publisher. Springer-Verlag Berlin Heidelberg. Copyright Holder. Springer- ... The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement ... Biology of Extracellular Matrix Free Preview © 2013. Evolution of Extracellular Matrix. Editors: Keeley, Fred W., Mecham, ...
Matrix mechanics and water permeation regulate extracellular vesicle transport | Nature Nanotechnology
Stress relaxation properties of the matrix as well as water transport through aquaporin-1 enable extracellular vesicles to ... which requires EVs to traverse the extracellular matrix (ECM). However, given that the size of EVs is usually larger than the ... Matrix stress relaxation allows EVs to overcome the confinement, and a higher crosslinking density facilitates a fluctuating ... Using engineered hydrogels, we demonstrate that the mechanical properties of the matrix regulate anomalous EV transport under ...
Extracellular Sugar-Based Biopolymers Matrices | Ephraim Cohen | Springer
Bacterial proteins binding to the mammalian extracellular matrix. - PubMed - NCBI
Many of the interactions have also been demonstrated on tissue sections or in vivo, and adherence to the extracellular matrix ... This review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the ... Some of these bacterial proteins are highly specific for an extracellular matrix protein, some are multifunctional and express ... Pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e ...
The extracellular matrix of the human optic nerve. - PubMed - NCBI
The extracellular matrix of the human optic nerve.. Goldbaum MH1, Jeng SY, Logemann R, Weinreb RN. ... The nerve fibers of the optic nerve are enclosed and segmented by extracellular matrix. With immunostains, we localized ... and fibronectin in frozen sections of the extracellular matrix of the prelaminar, laminar, and retrolaminar human optic nerve. ... The internal limiting lamina of the optic nerve has an extracellular composition similar to the thicker adjacent retinal ...
Keystone Meeting on The Extracellular Matrix
... Michael G. Hahn hahn at MOND1.CCRC.UGA.EDU Tue Nov 14 15:26:40 EST 1995 * ... Matrix Polysaccharides, Callose, and Cellulose Deborah Delmer, Bruce Wasserman, John Ralph Role of the Extracellular Matrix in ... roles of the extracellular matrix in plant development and in interaction= s with sybiotic and pathogenic organisms, and the ... The Extracellular Matrix of Plants: Molecular, Cellular and Developmental Biology Tamarron, Colorado. March 15-21, 1996 The ...
Complex bile duct network formation within liver decellularized extracellular matrix hydrogels | Scientific Reports
Here we demonstrate the ability of decellularized liver extracellular matrix (dECM) hydrogels to induce the in vitro formation ... Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels. Tissue Eng. Part A 16, 1075-1082 ... Complex bile duct network formation within liver decellularized extracellular matrix hydrogels. *Phillip L. Lewis1,2. , ... The extracellular matrix (ECM) of the developing liver plays an integral role in the formation and maturation of the biliary ...
Extracellular Matrix and Integrins in Cardiovascular Disease
Kliniken & Institute … Kliniken Zentrum für Innere… Innere Medizin III:… Forschung Grundlagenforschung … Extracellular Matrix… ... AG Extracellular Matrix and Integrins in Cardiovascular Disease. Integrin signaling and heart failure Integrins are ... By establishing a Tamoxifen (Tx) inducible knockout mouse, we have demonstrated that the extracellular matrix glycoprotein ... In this project we analyze effects of extracellular cues for hypertrophic remodeling and examine transcription factor circuits ...
Extracellular-matrix tethering regulates stem-cell fate
Interactions of human tenascin-X domains with dermal extracellular matrix molecules | SpringerLink
... is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and blood vessels. ... a new extracellular matrix glycoprotein localized on collagen fibrils. Matrix Biol 15:11-19PubMedCrossRefGoogle Scholar ... Tenascin-X (TNX) is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and ... Bristow J, Tee MK, Gitelman SE, Mellon SH, Miller WL (1993) Tenascin-X: a novel extracellular matrix protein encoded by the ...
Quantitative Analysis of Extracellular Matrix Formation in Vivo and in Vitro | SpringerLink
1988) Extracellular matrix specificity for the differentiation of capillary endothelial cells. Exp. Cell Res., 178: 426-434. ... 1991) Effects of soluble factors and extracellular matrix components on vascular cell behavior in vitro and in vivo: models of ... Madri, J.A., Pratt, B.M., and Yannariello-Brown, J.(1989) Endothelial cell-extracellular matrix interactions. In: Endothelial ... 1978) An ultrastructural study of developing extracellular matrix in vitelline blood vessels of the early chick embryo. Am. J. ...
The ECM-Cell Interaction of Cartilage Extracellular Matrix on Chondrocytes
In biology, the extracellular matrix (ECM) is the extracellular part of multicellular structure (e.g., organisms, tissues, and ... to pro-anabolic upon integration to the extracellular matrix," Matrix Biology, vol. 31, no. 5, pp. 290-298, 2012. View at ... P. G, "The extracellular matrix and cell adhesion," in Cells, B. Lewin, L. Cassimeris, V. Lingappa, and G. Plopper, Eds., 2007. ... "Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia," Matrix ...
extracellular matrix | Gastrointestinal and Liver Physiology
Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro | PNAS
1993) Extracellular matrix 3: Evolution of the extracellular matrix in invertebrates. FASEB J 7:1115-1123.. ... Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro. Yael Helman, Frank Natale, Robert ... 2000) Extracellular matrix (ECM) components in a very primitive multicellular animal, the dicyemid mesozoan Kantharella ... Extracellular production of organic matrices and calcium carbonate particles was examined in primary, nondividing cell cultures ...
JCI -
Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
Bonnans C, Chou J, Werb Z. Remodelling the extracellular matrix in development and disease. Nat Rev Mol Cell Biol. 2014;15(12): ... Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques. Sarah R. Langley,1,2 Karin ... The subendothelial extracellular matrix modulates JNK activation by flow. Circ Res. 2009;104(8):995-1003.. View this article ... Extracellular matrix composition and remodeling in human abdominal aortic aneurysms: a proteomics approach. Mol Cell Proteomics ...
COMPOSITION AND METHODS FOR REGULATING EXTRACELLULAR MATRIX ACCUMULATION - UNIVERSITY HEALTH NETWORK
There is described herein methods of treating a disease associated with extracellular matrix (ECM) in a patient. In some cases ... 1 shows extracellular matrix accumulation (ECM) in normal and fibrotic skin corresponds to a shift in metabolism from fatty ... 21 shows extracellular matrix deposition in the murine skin fibrosis after CD36high fibroblast vs. vehicle treatment. Tissue ... The skin is the largest organ of the human body and 70% of its dry weight is comprised of extracellular matrix (ECM). The ...
JCI -
Immobilization after injury alters extracellular matrix and stem cell fate
A protective extracellular matrix underpins HIV infectivity - Press release Institut Pasteur
A protective extracellular matrix underpins HIV infectivity. All press releases are available in the Press area of the Institut ... A protective extracellular matrix underpins HIV infectivity. A "viral biofilm" (in green) produced at the surface of a CD4 T ... HomePress areaPress documentsA protective extracellular matrix underpins HIV infectivity ... transported in an adhesive extracellular matrix, which also shelters them from the immune system and antiretroviral drugs. "We ...
Dystroglycan: an extracellular matrix receptor linked to the cytoskeleton
277e) The Impact of Decellularization Agents on Renal Tissue Extracellular Matrix | AIChE
Transitional Remodeling of the Hepatic Extracellular Matrix in Alcohol-Induced Liver Injury
The purpose of this review is to summarize the established and proposed changes to the hepatic extracellular matrix (ECM) that ... The fibrotic stage of ALD is primarily characterized by robust accumulation of extracellular matrix (ECM) proteins (collagens) ... "The extracellular matrix: tools and insights for the omics era," Matrix Biology, vol. 49, pp. 10-24, 2016. View at: Publisher ... The extracellular matrix (ECM) consists of a diverse range of components that work bidirectionally with surrounding cells to ...
ECM2 - Extracellular matrix protein 2 - Equus caballus (Horse) - ECM2 gene & protein
... extracellular matrix organization, positive regulation of cell-substrate adhesion ... Extracellular region or secreted. *extracellular matrix Source: GO_CentralInferred from biological aspect of ancestori*. " ... extracellular matrix organization Source: GO_CentralInferred from biological aspect of ancestori*. "Phylogenetic-based ... Extracellular matrix protein 2Imported. ,p>Information which has been imported from another database using automatic procedures ...
Frontiers | Shaping Synapses by the Neural Extracellular Matrix | Frontiers in Neuroanatomy
... and postsynaptic neuronal elements with astroglial processes and extracellular matrix (ECM) for formation and plasticity of ... Here we outline the major mechanisms driving: (i) synaptogenesis by secreted extracellular scaffolding molecules, like ... Here we outline the major mechanisms driving (i) synaptogenesis by secreted extracellular scaffolding molecules, like ... Particularly, we focused on potential importance of activity-dependent concerted activation of multiple extracellular proteases ...
Cutaneous extracellular matrix | HSTalks
Hironobu Fujiwara on Cutaneous extracellular matrix, part of a collection of online lectures. ... Cutaneous extracellular matrix. *Dr. Hironobu Fujiwara - RIKEN Center for Developmental Biology (CDB) Kobe, Japan ... Fujiwara, H. (2014, May 4). Cutaneous extracellular matrix [Video file]. In The Biomedical & Life Sciences Collection, Henry ... Today Id like to talk about several of the extracellular matrices, ECM in mammalian skin. ...
Matrix extracellular phosphoglycoprotein - wikidoc
Review articles on Matrix extracellular phosphoglycoprotein Articles on Matrix extracellular phosphoglycoprotein in N Eng J Med ... Ongoing Trials on Matrix extracellular phosphoglycoprotein at Clinical Trials.gov Trial results on Matrix extracellular ... Patient Handouts on Matrix extracellular phosphoglycoprotein Directions to Hospitals Treating Matrix extracellular ... Most recent articles on Matrix extracellular phosphoglycoprotein Most cited articles on Matrix extracellular ...
ProteinsProteinMacromoleculesProteasesAbstractCellsTissueMoleculesInteractionsCytoskeletonIntegrinsTissuesRole of the extracellular matrixDegradationMetalloproteinaseAdhesionPolymeric substancesLamininBasement membranesFibronectinFound within the extracellularMetalloproteinasesIntracellularCompositionDifferentiationStiffnessInterstitial matrixGlycoproteinsScaffoldMicroenvironmentFibersSignal transductionGeneBiologyMigrationCellularEpitheliumVitroSolubleGlycoproteinAlterationsMediatesMammalianRemodelingFibroblastsStructuralAMSBIOFilamentsMicroenvironmentsSpringerReceptor
Proteins37
- Glycosaminoglycans (GAGs) are carbohydrate polymers and mostly attached to extracellular matrix proteins to form proteoglycans (hyaluronic acid is a notable exception, see below). (wikipedia.org)
- In the extracellular matrix, especially basement membranes , the multi-domain proteins perlecan , agrin , and collagen XVIII are the main proteins to which heparan sulfate is attached. (wikipedia.org)
- The development of Corning Matrigel matrix, a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors. (corning.com)
- The proteins and glycoconjugates that make up the extracellular matrix provide structural support to cellular complexes, facilitate cell adhesion and migration, and impart mechanical properties that are important for tissue function. (springer.com)
- The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement membranes. (springer.com)
- The extracellular matrix (ECM) is an acellular three-dimensional network composed of proteins, glycoproteins, proteoglycans and exopolysaccharides. (springer.com)
- Bacterial proteins binding to the mammalian extracellular matrix. (nih.gov)
- Pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e. collagens, laminin, fibronectin or proteoglycans. (nih.gov)
- Some of these bacterial proteins are highly specific for an extracellular matrix protein, some are multifunctional and express binding activities towards a number of target proteins. (nih.gov)
- The scheduled sessions include those on structural studies of the major macromolecules that make up the plant extracellular matrix (polysaccharides, proteins, lignin), biosynthesis of wall macromolecules,= roles of the extracellular matrix in plant development and in interaction= s with sybiotic and pathogenic organisms, and the importance of plant cell walls= in food and industry. (bio.net)
- Cartilage extracellular matrix (ECM) is composed primarily of the network type II collagen (COLII) and an interlocking mesh of fibrous proteins and proteoglycans (PGs), hyaluronic acid (HA), and chondroitin sulfate (CS). (hindawi.com)
- This protective mesh is composed principally of proteins and carbohydrates and is comparable to that of bacterial biofilm, an extracellular matrix network secreted by some bacteria as protection against their environment. (pasteur.fr)
- 8,200 antibodies specific for proteins of the extracellular matrix. (antibodies-online.com)
- Background: The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. (cellsignal.com)
- Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). (cellsignal.com)
- The glue is fibronectin, a cell adhesion protein that allows cells to attach to the different matrix elements, including collagen, GAGs, and integrins, membrane proteins that connect cells to the environment and play an important role in signaling cascades. (jove.com)
- ECM2 encodes extracellular matrix protein 2, so named because it shares extensive similarity with known extracellular matrix proteins. (wikipedia.org)
- AlphaD1 exits only of Alpha-helices, whereas SASDL2 and -3 are capable of binding most of the extracellular matrix proteins identified so far (collagen type IV, laminin 332, fibronectin , perlecan, fibulin 1C/D , fibulin-3 and MMP-9 ) (Sercu et al. (atlasgeneticsoncology.org)
- Macromolecular crowding in extracellular culture media directly induced supramolecular assembly and alignment of extracellular matrix proteins deposited by cells, which in turn increased alignment of the intracellular actin cytoskeleton. (mit.edu)
- This new edition of Guidebook to the Extracellular Matrix and Adhesion Proteins brings up-to-date information on the purification, activities, antibodies and genes for the proteins found in the extracellular matrix and those known to be involved in cell-cell contact and adhesion. (google.it)
- Drawing on a wide range of sources, this volume addresses all aspects of the structure and function of modules in the extracellular matrix, adhesion molecules, cell adhesion and cell-cellcontact proteins. (google.it)
- CD36 is a busy molecule, regularly making contact with a wide variety of proteins in the extracellular matrix and on neighboring cells. (thefreedictionary.com)
- The carboxy terminus of dystrophin binds to a plasma membrane anchor, [beta]-dystroglycan, which is associated on the external side with the extracellular matrix receptor, [alpha]-dystroglycan, which binds to the basal lamina proteins laminin 1, laminin 2, and agrin. (thefreedictionary.com)
- Thus interaction between integrins and specific matrix proteins is responsible for sensing mechanical strain in vascular smooth muscle cells. (jci.org)
- to investigate the lymphocyte pathways regulating the viral biofilms formation and composition in extracellular matrix (ECM) proteins (using quantitative polymerase chain reaction (qPCR) and siRNA). (clinicaltrials.gov)
- In this study we explored the effect of selective extracellular matrix (ECM) proteins on the potential of insulin-producing cell differen-tiation using ARIP cells, an adult rat pancreatic ductal epithelial cell line, as a model in vitro. (scirp.org)
- Is the Subject Area "Extracellular matrix proteins" applicable to this article? (plos.org)
- For example, matrix proteins sequester water that provides turgor to soft tissues and minerals that give rigidity to skeletal tissues. (humpath.com)
- neurofascin-186 [NF-186] and neuron glia-related CAM [NrCAM]), cytoskeletal proteins (ankyrinG and βIV spectrin), and the extracellular chondroitin-sulfate proteoglycan brevican. (rupress.org)
- Thus, the clustering of ion channels at nodes of Ranvier is thought to depend on binding to cytoskeletal and scaffolding proteins that are positioned along axons by extracellular, heterophilic interactions between axonal and glial CAMs ( Schafer and Rasband, 2006 ). (rupress.org)
- Rather than linking two cells, hemi-desmosomes attach cells to the extracellular matrix and use integrin cell adhesion proteins rather than cadherins. (sabiosciences.com)
- We have shown that, in vivo , SCLC cells are surrounded by an extensive stroma of extracellular matrix (ECM) at both primary and metastatic sites which contains, among other proteins, fibronectin, laminin and collagen IV. (clinsci.org)
- Next, cell migration uses adhesion proteins on both the cell surface and in the extracellular matrix (ECM) to guide remodeling and cell movement through the environment. (jimmunol.org)
- Tissue inhibitors of metalloproteinases (TIMPs) regulate the ECM turnover through negative regulation of matrix metalloproteinases (MMPs), which degrade the ECM structural proteins. (ahajournals.org)
- 3 - 7 Matrix metalloproteinases (MMPs) degrade ECM proteins, whereas their proteolytic activity is kept in check by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). (ahajournals.org)
- Proteolytic assays were performed with extracellular matrix proteins to assess the putative role of Cwp84 in the pathogenicity of C. difficile . (asm.org)
- P47 shows strong and specific binding to gastrointestinal tissues and some extracellular matrix proteins (type I collagen, thrombospondin, and vitronectin) ( 4 ). (asm.org)
Protein17
- In fact, collagen is the most abundant protein in the human body [15] [16] and accounts for 90% of bone matrix protein content. (wikipedia.org)
- Higher protein concentration provides greater matrix stiffness and scaffold integrity. (corning.com)
- The other protein components of the BM are thought to be non-covalently immobilized in the matrix formed by laminin and collagen IV6. (sigmaaldrich.com)
- However, cells on polyacrylamide of low elastic modulus (0.5 kPa) could not form stable focal adhesions and differentiated as a result of decreased activation of the extracellular-signal-related kinase (ERK)/mitogen-activated protein kinase (MAPK) signalling pathway. (nih.gov)
- Tenascin-X (TNX) is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and blood vessels. (springer.com)
- Bristow J, Tee MK, Gitelman SE, Mellon SH, Miller WL (1993) Tenascin-X: a novel extracellular matrix protein encoded by the human XB gene overlapping P450c21B. (springer.com)
- In this regard, especially the matrix protein Tenascin C (Tnc) proved to be an important regulator of astrocyte precursor cell proliferation and migration during spinal cord development. (frontiersin.org)
- Collagen I is a heterotrimeric extracellular matrix protein found in the skin and other connective tissues. (thermofisher.com)
- Extracellular matrix protein 2 is a protein that in humans is encoded by the ECM2 gene. (wikipedia.org)
- We quantified the impact of induced crowding on the extracellular and intracellular protein organization of human mesenchymal stem cells (MSCs) via immunocytochemistry, atomic force microscopy (AFM), and AFM-enabled nanoindentation. (mit.edu)
- Cysteine rich 61 (CCN1) is an extracellular matrix (ECM) protein elevated in cancer cells that modulates their adhesion and migration by binding cell surface receptors. (aacrjournals.org)
- It is a secreted protein which typically localizes in the extracellular matrix (ECM) and on the cell surface ( 5 ), in which it binds integrin receptors to modulate a variety of cellular functions including adhesion, migration, and proliferation ( 6 ). (aacrjournals.org)
- Cells adhered, spread, and proliferated on each matrix protein, but the mitogenic response to strain was matrix dependent. (jci.org)
- Here, the use of the C-C motif chemokine ligand 25 (CCL)25 in comparison to differentiation factors such as transforming growth factor (TGF)β3, bone morphogenetic protein (BMP)2, BMP7, BMP12, and BMP14 (all in concentrations of 10, 50 and 100 ng/mL) was tested in an in vitro micro mass pellet model with isolated and cultivated human AF-cells ( n = 3) to induce and enhance AF-matrix formation. (mdpi.com)
- This work demonstrates a role for a brain-specific extracellular matrix protein in glioma invasion, opening new therapeutic avenues for a uniformly fatal disease. (jneurosci.org)
- We now show that JNK activation by both onset of laminar flow and long-term oscillatory flow is matrix-specific, with enhanced activation on fibronectin compared to basement membrane protein or collagen. (ahajournals.org)
- The composition of the matrix of the interstitium and the proportion and the expression of each protein can have a profound influence on cardiac structure and compliance that will determine its hemodynamic functions ( 20 ). (physiology.org)
Macromolecules7
- In biology , the extracellular matrix ( ECM ) is a three-dimensional network of extracellular macromolecules , such as collagen , enzymes , and glycoproteins , that provide structural and biochemical support of surrounding cells. (wikipedia.org)
- In biology, the extracellular matrix (ECM) is a three-dimensional network consisting of extracellular macromolecules and minerals, such as collagen, enzymes, glycoproteins and hydroxyapatite that provide structural and biochemical support to surrounding cells. (wikipedia.org)
- The Extracellular matrix (ECM) is the network of extracellular macromolecules that provide cells structural and biochemical support. (peprotech.com)
- These macromolecules are present in intercellular junctions and cell surfaces and may assemble into two general organizations: interstitial matrix and basement membrane (BM). (humpath.com)
- The cells in tissue are in contact with secreted extracellular macromolecules referred to as the extracellular matrix . (humpath.com)
- Thus, extracellular macromolecules influence and organize not only the basal cell surface but also the actin-rich basal cell cortex of epithelial cells. (rupress.org)
- the myocardial extracellular matrix (ECM) represents a complex three-dimensional network of various macromolecules. (physiology.org)
Proteases6
- This volume in the Biology of Extracellular Matrix series provides a review of the known classes of proteases that degrade ECM both outside and inside the cell. (springer.com)
- The topics covered in this volume provide an important context for understanding the role that matrix-degrading proteases play in normal tissue remodeling and in diseases such as cancer and lung disease. (springer.com)
- Comparative genomics has identified a conserved core ECM adhesome, that includes integrin adhesion receptors, matrix proteases and the central ECM components of connective ECM and basement membranes. (els.net)
- Collagen is exocytosed in precursor form (procollagen), which is then cleaved by procollagen proteases to allow extracellular assembly. (wikipedia.org)
- Particularly, we focused on potential importance of activity-dependent concerted activation of multiple extracellular proteases, such as ADAMTS4/5/15, MMP9 and neurotrypsin, for permissive and instructive events in synaptic remodeling through localized degradation of perisynaptic ECM and generation of proteolytic fragments as inducers of synaptic plasticity. (frontiersin.org)
- Among proteases, the matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family are often associated with ECM degradation and remodeling. (sabiosciences.com)
Abstract1
- The abstract deadline for the Keystone Meeting on The Extracellular Matri= x of Plants: Molecular, Cellular and Developmental Biology HAS BEEN EXTENDED to November 20, 1995. (bio.net)
Cells54
- [5] Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). (wikipedia.org)
- [7] Some single-celled organisms adopt multicellular biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS). (wikipedia.org)
- Cells behave better on Corning Matrigel matrix-the original, trusted extracellular matrix (ECM). (corning.com)
- The evolution of single cells into multicellular organisms was mediated, in large part, by the extracellular matrix. (springer.com)
- Cells release extracellular vesicles (EVs) to communicate over long distances, which requires EVs to traverse the extracellular matrix (ECM). (nature.com)
- The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. (sigmaaldrich.com)
- Here we demonstrate the ability of decellularized liver extracellular matrix (dECM) hydrogels to induce the in vitro formation of complex biliary networks using encapsulated immortalized mouse small biliary epithelial cells (cholangiocytes). (nature.com)
- Cells attach to proteoglycans and glycoproteins on the surface of other cells as well as in the extracellular matrix (ECM) substratum via adhesion molecules to define tissue shape, structure, and function. (qiagen.com)
- In biology, the extracellular matrix (ECM) is the extracellular part of multicellular structure (e.g., organisms, tissues, and biofilms) that typically provides structural and biochemical support to the surrounding cells [ 1 ]. (hindawi.com)
- Integrins are transmembrane receptors that mediate the attachment between a cell and its surroundings, such as other cells or the extracellular matrix (ECM). (hindawi.com)
- The evolution of multicellularity in animals required the production of extracellular matrices that serve to spatially organize cells according to function. (pnas.org)
- In this report, we examine the production of these three matrices by using an in vitro culturing system for coral cells. (pnas.org)
- Using HIV-infected CD4+ T cells, Dr Thoulouze and colleagues analyzed the extracellular mesh formed at the surface of these cells and evaluated viral infection after its destruction. (pasteur.fr)
- Effective decellularization uses agents that lyse cells and remove all cellular materials, leaving intact collagenous extracellular matrices (ECMs). (aiche.org)
- The agreement is based upon Kensey Nash 's proprietary tissue processing technology, known as the Optrix process, which gently disinfects tissues, inactivates viruses and removes cells while preserving extracellular matrix components. (medindia.net)
- Human prolactin secreting adenoma cells maintained on extracellular matrix. (biomedsearch.com)
- In vitro maintenance of human prolactin secreting adenoma cells on a extracellular matrix (ECM) produced by bovine corneal endothelial cells is associated with the adoption of morphological properties that are not expressed when the cells are seeded on plastic. (biomedsearch.com)
- Both tumor cells and normal cells are more likely to resemble their in vivo counterparts when maintained on extracellular matrix than on plastic, and therefore, cells cultured on ECM may provide a better system for oncogenic and endocrine studies. (biomedsearch.com)
- In biology, the extracellular matrix ( ECM ) is the extracellular part of animal tissue that usually provides structural support to the animal cells in addition to performing various other important functions. (medicalxpress.com)
- Methods for culturing eukaryotic cells and studying their growth characteristics, including the invasive growth characteristics of tumor cells, on the submucosal matrix are described. (google.com)
- Supporting many cellular tissues is the extracellular matrix, or ECM, an interconnected network composed of fibers and ground substance, which is mostly interstitial fluid that fills the space between the cells, connective tissue fibers, and capillaries. (jove.com)
- In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). (jove.com)
- Geltrex™ matrix is a soluble form of reduced growth factor (RGF) basement membrane extract (BME) purified from continuous sheets of specialized extracellular matrix that form an interface between Engelbreth-Holm-Swarm (EHS) tumor cells. (thermofisher.com)
- Coloured scanning electron micrograph (SEM) of the extracellular matrix (ECM) found around multicellular aggregates of yeast cells, such as biofilms. (sciencephoto.com)
- Are factors originating from serum, plasma, or cultured cells involved in the growth-promoting effect of the extracellular matrix produced by cultured bovine corneal endothelial cells? (thefreedictionary.com)
- The molecules of the extracellular matrix (ECM) comprise the connective tissues of animals and form basal laminae underlying sheets of epithelial cells. (thefreedictionary.com)
- Our work elucidated a highly intriguing question: how cells move when they are in the complex and physiologically relevant environment of a 3-D extracellular matrix ," said Hyun (Michel) Koo, a professor in the Department of Orthodontics at Penn's School of Dental Medicine. (thefreedictionary.com)
- Mechanical strain of rat vascular smooth muscle cells is sensed by specific extracellular matrix/integrin interactions. (jci.org)
- 7. The biomaterial scaffold of claim 1, wherein the reconstituted extracellular matrix is derived from cultured cells or animal tissue. (freepatentsonline.com)
- 21. A biomaterial scaffold comprising reconstituted extracellular matrix, polyelectrolyte complex fibers and seeded cells, wherein the extracellular matrix is derived from the same or similar cell type as the seeded cells. (freepatentsonline.com)
- It is not clear whether the extracellular matrix plays only a passive role or a more active, instructive role in shaping organs, in part, because it is difficult to measure the physical forces within densely packed cells. (elifesciences.org)
- This work reveals that, along with providing structural support to cells, the mechanical properties of the matrix also actively guide how organs form. (elifesciences.org)
- resistance to these forces is mediated by cells and by the extracellular matrix (ECM), including the basement membranes (BMs) that line all epithelia. (elifesciences.org)
- Here, though, and increasingly in scientific and research circles and professionals worldwide, 'fascia' has a wider definition: all the collagenous-based soft-tissues in the body, including the cells that create and maintain that network of extra-cellular matrix (ECM). (anatomytrains.com)
- The composition of the extracellular matrix may be a critical factor in determining the invasive potential of cancer cells, such that the production of matrix elements by glioma cells might mediate their invasion into normal tissue. (jneurosci.org)
- Behaviors characteristic of cells in the developing brain, such as cell proliferation and migration, neuronal and glial process outgrowth, and the elaboration of the capillary network, take place in a soluble matrix that is permissive for cell movement. (jneurosci.org)
- A model of extracellular matrix (ECM) of collagen fibers has been built, in which cells could communicate with distant partners via fiber-mediated long-range-transmitted stress states. (aps.org)
- The Extracellular Matrices and Substrates are a group of products that provide such support for 2D and 3D cell culture research and allow for more options to provide the most in vivo -like environment for the specific cells that are being used. (peprotech.com)
- The extra-cellualr matrix (ECM) is secreted locally and assembles into a network in the spaces surrounding cells (intercellualr space). (humpath.com)
- The interstitial matrix is present in spaces between epithelial, endothelial, and smooth muscle cells and in connective tissue . (humpath.com)
- The matrix helps hold the cells and tissues together and provides a means in which cell can migrate and interact. (humpath.com)
- In vivo, cells are exposed to mechanical forces as a consequence of blood flow, interstitial flow, blood pressure, and extracellular matrix (ECM) stiffness. (sciencemag.org)
- Matrix metalloproteinase (MMP)-1, -3 and MMP-13 expression was reduced in aging ACL but increased in degenerated ACL, mainly in the chondrocyte-like cells. (biomedsearch.com)
- Tight junctions prevent the movement of the extracellular matrix between cells. (jiskha.com)
- The nonliving matrix of the connective tissue through which the blood cells freely flow is the? (jiskha.com)
- Extracellular matrix provides the microenvironment for the cells and serves as a tissue scaffold, guiding cell migration during embryonic development and wound repair. (sabiosciences.com)
- The features of the ECM are determined both by the cells that produce the matrix and by the cells growing in it. (sabiosciences.com)
- Cell adhesion is the binding of the cells to each other and to the extracellular matrix through cell adhesion molecules (CAMs) such as integrins, selectins, cadherins, the Ig (immunoglobulin) superfamily, and lymphocyte homing receptors. (sabiosciences.com)
- Cancer cell invasion and metastasis is a complex, multistep process involving interactions between invading cells, the extracellular matrix, and other stromal elements ( 1 ). (aacrjournals.org)
- Proteolytic enzymes secreted by tumor and/or host cells are required for cancer cells to invade the extracellular matrix and infiltrate lymphatic or blood vessel walls to metastasize to regional or distant sites. (aacrjournals.org)
- It is enriched on the surface of tumor cells and stimulates the production of matrix metalloproteinases (MMPs) by adjacent stromal cells. (aacrjournals.org)
- The collagen fibrils that these cells synthesise provide a template for postnatal growth by structure-based matrix expansion. (elifesciences.org)
- Extracellular matrix (ECM) mechanical stiffness and its dynamic change is one of the main cues that directly affects the differentiation and proliferation of normal cells as well as the progression of disease processes such as fibrosis and cancer. (rsc.org)
- The injured tissue environment upon sensing the stress turns on a precisely orchestrated network of immune responses by regulating cytokine-chemokine production, recruitment of immune cells, and modulating fibrogenic niche and extracellular matrix (ECM) cross-talk during fibrotic pathologies like cardiac fibrosis, liver fibrosis, laryngotracheal stenosis, systemic scleroderma, interstitial lung disease and inflammatory bowel disease. (intechopen.com)
Tissue22
- Regulated turnover of extracellular matrix (ECM) is an important component of tissue homeostasis. (springer.com)
- Many of the interactions have also been demonstrated on tissue sections or in vivo, and adherence to the extracellular matrix has been shown to promote bacterial colonization of damaged tissues. (nih.gov)
- They orchestrate cell-cell and cell-matrix adhesive interactions from embryonic development to mature tissue function. (hindawi.com)
- Cartilaginous ECM is remodeled continuously by a combination of production, degradation by matrix metalloproteinases (MMPs), and inhibition of MMPs activity by tissue inhibitors of MMPs [ 4 ]. (hindawi.com)
- The metazoan extracellular matrix (ECM), an extracellular system of insoluble networks, sheets and fibrils of proteinaceous material, is a central mediator of multicellularity that is required for developmental processes, tissue organisation, homoeostasis and control of cell phenotypes throughout life. (els.net)
- The extracellular matrix is the defining feature of connective tissue in animals. (medicalxpress.com)
- This conference will focus on the converging roles of the extracellular matrix (ECM) and soluble factors for the repair or replacement of tissue lost or damaged due to degenerative disease, cancer, or trauma. (grc.org)
- To isolate extracellular matrix from the kidney, place the diced cortex tissue into the beaker containing SDS solution. (jove.com)
- Studies based on Efemp1 knock-out mouse implicate EFEMP1 function in withholding tissue integrity by stimulating the expression of Timp1 and Timp3 and inhibiting the expression and activities of matrix metalloproteinase Mmp2 , and Mmp9 (Rahn et al. (atlasgeneticsoncology.org)
- In this educational video, Dr. Marc Gerdisch of the St. Francis Heart Center describes extracellular matrix and how it is being used to enhance tissue function in repaired mitral valves. (heart-valve-surgery.com)
- The extracellular matrix is considered one of the defining features of connective tissue in animals. (heart-valve-surgery.com)
- The extra-cellualr matrix (ECM) forms a significant proportion of the volume of any tissue. (humpath.com)
- Tissue and extracellular matrix (ECM) stiffness is transduced into intracellular stiffness, signaling, and changes in cellular behavior. (sciencemag.org)
- Preparation and Characterization of a Chitosan/Gelatin/Extracellular Matrix Scaffold and Its Application in Tissue Engineering. (sigmaaldrich.com)
- Previous studies have demonstrated that extracellular matrix (ECM) can be used in tissue engineering due to its bioactivity. (sigmaaldrich.com)
- Loss of flexibility is due at least in part to chemical changes in the Extracellular Matrix (ECM) in the tissue, converting a flexible network of molecules into a rigid mesh. (ddw-online.com)
- The ability of tendons to transmit such large forces is directly attributable to an extracellular matrix (ECM) comprising collagen fibrils aligned parallel to the tissue long axis. (elifesciences.org)
- However, the absence of a robust method of imaging cell-matrix organisation has precluded a detailed study of how the cell-rich anlage found in embryogenesis grows into a mechanically strong connective tissue in the adult. (elifesciences.org)
- Additionally, all tissue-based neutrophilic responses to fungal infections necessitate contact with the extracellular matrix (ECM). (jimmunol.org)
- 56% women), we related circulating concentrations of matrix metalloproteinase-9 (binary variable: detectable versus undetectable), log of tissue inhibitor of matrix metalloproteinase-1, and log of procollagen type III aminoterminal peptide (PIIINP) to incident CVD and death. (ahajournals.org)
- Each SD increment of log of tissue inhibitor of matrix metalloproteinase-1 and log-PIIINP was associated with multivariable-adjusted hazards ratios of 1.72 (95% CI, 1.30 to 2.27) and 1.47 (95% CI, 1.11 to 1.96), respectively, for mortality risk. (ahajournals.org)
- Conclusion- Higher circulating tissue inhibitor of matrix metalloproteinase-1 and PIIINP concentrations are associated with mortality, and higher PIIINP is associated with incident CVD, in the community. (ahajournals.org)
Molecules7
- The Human Extracellular Matrix & Adhesion Molecules RT² Profiler PCR Array profiles the expression of 84 genes important for cell-cell and cell-matrix interactions. (qiagen.com)
- The Mouse Extracellular Matrix & Adhesion Molecules RT² Profiler PCR Array profiles the expression of 84 genes important for cell-cell and cell-matrix interactions. (qiagen.com)
- The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules. (jove.com)
- However, the early astrocytes influence their environment not only by releasing and responding to diverse soluble factors but also express a wide range of extracellular matrix (ECM) molecules, in particular proteoglycans of the lectican family and tenascins. (frontiersin.org)
- EFEMP1 is one member of fibulins that serve to modulate cellular behavior and functions by connecting and integrating multiple partner molecules in extracellular compartment. (atlasgeneticsoncology.org)
- The Extracellular Matrix and Cell Adhesion PCR Arrays are designed to determine the gene expression profile of the molecules involved in cell-to-cell and cell-to-matrix interactions. (sabiosciences.com)
- Response of basal epithelial cell surface and Cytoskeleton to solubilized extracellular matrix molecules. (rupress.org)
Interactions5
- This review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the biological significance of these interactions. (nih.gov)
- Accumulating data support the importance of interactions between pre- and postsynaptic neuronal elements with astroglial processes and extracellular matrix (ECM) for formation and plasticity of chemical synapses, and thus validate the concept of a tetrapartite synapse. (frontiersin.org)
- This method can help answer key questions in the field of biomaterials, such as how cell matrix interactions affect sulfate. (jove.com)
- For a basic understanding of the chemical and physical interactions of the EPS matrix, model biofilms with increasing complexity are designed and the influence of structure and composition on the physical properties is investigated. (wur.nl)
- Finally, we determined the functional importance of integrin-fibronectin interactions and matrix metalloprotease (MMP)-facilitated cell movement during re-epithelialization and propose a model for cell locomotion during postmenstrual repair. (bioone.org)
Cytoskeleton4
- Articular cartilage ECM plays a crucial role in regulating chondrocyte metabolism and functions, such as organized cytoskeleton through integrin-mediated signaling via cell-matrix interaction. (hindawi.com)
- Thus, NF-186 assembles and links the specialized brevican-containing AIS extracellular matrix to the intracellular cytoskeleton. (rupress.org)
- His work ties in with that of Donald Ingber on tensegrity within the cytoskeleton, and the links between the cytoskeleton and the Extra Cellular Matrix as described by James Oschman. (indigo.ca)
- Integrins are heterodimers of α- and β - subunits that bind to the ECM through large extracellular domains and connect intracellularly to the actin cytoskeleton filaments. (sabiosciences.com)
Integrins2
- I ) In the absence of extracellular signals, BMPRs and integrins do not engage without ligand binding. (portlandpress.com)
- Subsequent binding of newly activated integrins to extracellular matrix (ECM) initiates downstream signals. (ahajournals.org)
Tissues4
- Hyaluronic acid in the extracellular space confers upon tissues the ability to resist compression by providing a counteracting turgor (swelling) force by absorbing significant amounts of water. (wikipedia.org)
- The expression of these gelatinases is relatively low in normal tissues and is induced when extracellular matrix remodeling is required. (aacrjournals.org)
- The interstitial ECM, which makes up most of the extracellular mass of the body, consists of many different types of collagen, tenascin, proteoglycans and elastin in elastic tissues such as skin or tendon. (ddw-online.com)
- Recent advancements in biomaterials have enabled a wide range of polymer matrices that could mimic the ECM of different tissues for a wide range of in vitro basic research and drug discovery. (rsc.org)
Role of the extracellular matrix1
- However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. (mcponline.org)
Degradation3
- The podosome rosettes set the scene for global extracellular matrix degradation and internalization. (thefreedictionary.com)
- The extracellular matrix (ECM) is a complex network of different combinations of collagens, proteoglycans, hyaluronic acid, laminin, fibronectin, and many other glycoproteins, including proteolytic enzymes involved in degradation and remodeling of the extracellular matrix. (sabiosciences.com)
- Matrix metalloproteinases (MMP), especially MMP2, are commonly overexpressed in squamous cell carcinoma and are known to promote invasion through degradation of collagen in the basement membrane. (aacrjournals.org)
Metalloproteinase4
- Matrix metalloproteinase-2 is required for the switch to the angiogenic phenotype in a tumor model," Proceedings of the National Academy of Sciences of the United States of America , vol. 97, no. 8, pp. 3884-3889, 2000. (hindawi.com)
- Similar results were found for matrix metalloproteinase (MMP)-3 (765 vs 370 pg·mL −1 , p=0.004). (ersjournals.com)
- The molecular mechanism of SCCRO-mediated effects on matrix metalloproteinase-2 (MMP2) levels and activity were assessed using a combination of cell biological and molecular methods, including real-time PCR, reporter assay, RNA interference, and chromatin immunoprecipitation assay. (aacrjournals.org)
- Matrix metalloproteinase-9 was unrelated to either outcome. (ahajournals.org)
Adhesion3
- QIAGEN provides a broad range of assay technologies for extracellular matrix and adhesion molecule research that enable analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. (qiagen.com)
- The resulting cell-matrix reciprocity further affected adhesion, proliferation, and migration behavior of MSCs. (mit.edu)
- Cell adhesion mediates cell attachment, migration, and signaling to and from the extracellular matrix. (sabiosciences.com)
Polymeric substances2
- The attachment and growth of bacteria on these membranes results in formation of biofilms: to create a favourable living environment they immobilize themselves in a slimy matrix of extracellular polymeric substances (EPS). (wur.nl)
- Down: Microscopic image of a biofilm, showing bacteria and extracellular polymeric substances. (wur.nl)
Laminin3
- With immunostains, we localized collagen types I through VI, laminin, and fibronectin in frozen sections of the extracellular matrix of the prelaminar, laminar, and retrolaminar human optic nerve. (nih.gov)
- When strain was applied on matrices containing both laminin and vitronectin, the mitogenic response to strain depended upon the vitronectin content of the matrix. (jci.org)
- The extracellular ligands that anchor these adhesions include laminin, fibronectin, vitronectin, and various collagens. (sabiosciences.com)
Basement membranes1
- EFEMP1 is likely to contribute the integrity of basement membrane zones and anchor other extracellular matrix structures such as elastic fibers to basement membranes. (atlasgeneticsoncology.org)
Fibronectin1
- By establishing a Tamoxifen (Tx) inducible knockout mouse, we have demonstrated that the extracellular matrix glycoprotein fibronectin (Fn), activates critical survival signals via ß1 integrin following myocardial infarction (1). (uni-heidelberg.de)
Found within the extracellular2
- Described below are the different types of proteoglycan found within the extracellular matrix. (wikipedia.org)
- A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. (umassmed.edu)
Metalloproteinases3
- L. M. Coussens and Z. Werb, Matrix metalloproteinases and the development of cancer ,, Chemistry and Biology , 3 (1996), 895. (aimsciences.org)
- Matrix metalloproteinases (MMPs) degrade ECM components and regulate normal cell migration as well as tumor cell invasion and metastases ( 5 , 6 ). (jimmunol.org)
- Several studies suggest that matrix metalloproteinases (MMP), a family of zinc-dependent endopeptidases, play a significant role in extracellular matrix invasion ( 3 ). (aacrjournals.org)
Intracellular1
- Therefore, tight extracellular and intracellular regulation of BMP signaling is critical. (portlandpress.com)
Composition4
- The internal limiting lamina of the optic nerve has an extracellular composition similar to the thicker adjacent retinal internal limiting lamina. (nih.gov)
- The invention relates to extracellular matrix (ECM) accumulation and more particularly to composition and methods for either increasing or decreasing the same. (freepatentsonline.com)
- Here we present a protocol to fabricate a kidney cortex extracellular matrix-derived hydrogel to retain the native kidney extracellular matrix (ECM) structural and biochemical composition. (jove.com)
- Bosman FT, Stamenkovic I. Functional structure and composition of the extracellular matrix. (humpath.com)
Differentiation2
- Native extracellular matrix preserves mesenchymal stem cell 'stemness' and differentiation potential under serum-free culture conditions. (nature.com)
- The investigators' preliminary data indicate that besides ' free ' infectious viral particles, HIV-1 infected cluster of differentiation 4 (CD4+) lymphocytes also produce extracellular viral assemblies wrapped in an extracellular matrix cocoon and tightly bound to the surface of the cell. (clinicaltrials.gov)
Stiffness1
- Kohn JC, Zhou DW, Bordeleau F, Zhou AL, Mason BN, Mitchell MJ, King MR, Reinhart-King CA. Cooperative effects of matrix stiffness and fluid shear stress on endothelial cell behavior. (umassmed.edu)
Interstitial matrix3
- The animal extracellular matrix includes the interstitial matrix and the basement membrane . (wikipedia.org)
- Second, I'll talk about the skin ECM, which comprises a basement membrane and interstitial matrix. (hstalks.com)
- Extracellular matrix (ECM) exists in two forms: interstitial matrix that fills in the intercellular space and the more specialized basement membrane, which is a thin sheet of extracellular matrix underlying the epithelium. (sabiosciences.com)
Glycoproteins2
- The extracellular matrix (ECM) is an insoluble network of collagens, glycoproteins, proteoglycans and glycosaminoglycans. (els.net)
- Extracellular matrix (ECM) contains collagen and elastic fibers embedded in a viscoelastic gel consisting of proteoglycans (PGs), hyaluronan and glycoproteins ( 29 ). (scielo.cl)
Scaffold2
- 4. The biomaterial scaffold of claim 2 wherein reconstituted extracellular matrix is incorporated into the polycation precursor and the polyanion precursor. (freepatentsonline.com)
- 11. The biomaterial scaffold of claim 1, wherein the reconstituted extracellular matrix is derived from an osteoblast cell line or a hepatocarcinoma cell line. (freepatentsonline.com)
Microenvironment2
- MAPTrix™ produces a uniform ECM surface that provides a highly controlled 2D extracellular microenvironment for cell cultures and related applications. (amsbio.com)
- A promising tool for the in vitro exploration of extracellular microenvironment (ECM) factors that regulate cell functions is the combinatorial library approach. (amsbio.com)
Fibers4
- The nerve fibers of the optic nerve are enclosed and segmented by extracellular matrix. (nih.gov)
- The extracellular matrix consists of fibers and a basic compound. (antibodies-online.com)
- 6 ]. It must be noted that tenocytes require a long time to produce a dense, collagen-rich neo-matrix consisting of parallel aligned type I collagen fibers. (mdpi.com)
- wherein the matrix and the fibers are functionally associated. (freepatentsonline.com)
Signal transduction2
- The Gordon Research Conference (GRC) on Signal Transduction by Engineered Extracellular Matrices was established in 2000 to enhance the intersection of bioengineering and cell biology with an aim to foster translation of cell-based technologies into practice. (grc.org)
- This GRC will be held in conjunction with the "Signal Transduction by Engineered Extracellular Matrices (GRS)" Gordon Research Seminar (GRS). (grc.org)
Gene2
- Matrix, extracellular phosphoglycoprotein with ASARM motif (bone) , also known as MEPE , is a human gene . (wikidoc.org)
- Greenhouse DG, Murphy A, Mignatti P, Zavadil J, Galloway AC, Balsam LB. Mitral valve prolapse is associated with altered extracellular matrix gene expression patterns. (umassmed.edu)
Biology6
- The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology. (springer.com)
- 3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D ANNOUNCEMENT (Reminder) Keystone Symposium: The Extracellular Matrix of Plants: Molecular, Cellular and Developmental Biology Tamarron, Colorado. (bio.net)
- In particular, this meeting will focus on the role that engineered matrices can play in advancing developmental biology and in vitroorganogenesis, single cell biology, microphysiological models and biofabrication ( i.e. "organs-on-chips"), regenerative medicine therapies, and immunology to study human health and disease. (grc.org)
- Jemma Evans , Tu'uhevaha Kaitu'u-Lino , and Lois A. Salamonsen "Extracellular Matrix Dynamics in Scar-Free Endometrial Repair: Perspectives from Mouse In Vivo and Human In Vitro Studies," Biology of Reproduction 85(3), 511-523, (25 May 2011). (bioone.org)
- The basement membrane (BM) and extracellular matrix (ECM) play critical roles in developmental and cancer biology, and are of great interest in biomathematics. (aimsciences.org)
- 6 Division of Pulmonary, Allergy and Critical Care Medicine, Program in Protease and Matrix Biology, Gregory Fleming James Cystic Fibrosis Centre and Lung Health Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA. (sciencemag.org)
Migration2
- We aimed to characterize and determine the importance of extracellular matrix (ECM) dynamics in cell migration during endometrial repair. (bioone.org)
- Microarray analysis revealed that in addition to modulating many of the same DC maturation genes as LPS, galectin-1 also uniquely up-regulated a significant subset of genes related to cell migration through the extracellular matrix (ECM). (jimmunol.org)
Cellular2
- Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis. (biomedsearch.com)
- Hypoxia promotes extracellular matrix (ECM) remodeling, cellular metabolic adaptation, and cancer cell metastasis. (sciencemag.org)
Epithelium1
- Corneal epithelium removed from underlying extracellular matrix (ECM) extends numerous cytoplasmic processes (blebs) from the formerly smooth basal surface. (rupress.org)
Vitro2
- We further demonstrate in vitro production of SOM and extracellular mineralized particles in cell cultures of M. digitata . (pnas.org)
- reported that in vitro a specialized brevican-containing matrix surrounds the AIS. (rupress.org)
Soluble2
- A return to an immature, more soluble matrix during tumor growth could facilitate tumor cell motility and angiogenesis. (jneurosci.org)
- This study examines the effect of soluble extracellular matrix components on the basal surface. (rupress.org)
Glycoprotein1
- Lethias C, Descollonges Y, Boutillon MM, Garrone R (1996) Flexilin: a new extracellular matrix glycoprotein localized on collagen fibrils. (springer.com)
Alterations2
- In this minireview, we will examine key extracellular matrix and matricellular components, their changes with aging, and discuss how these alterations might influence the subsequent progression of cancer in the aged host. (wiley.com)
- Dynamic alterations in extracellular matrix expression are essential for endometrial repair. (bioone.org)
Mediates3
- Furthermore, water permeation through aquaporin-1 mediates the EV deformability, which further supports EV transport in hydrogels and a decellularized matrix. (nature.com)
- Fig. 4: Aquaporin-1 mediates the ability of EVs to transport in engineered and decellularized matrices by increasing the EV deformability. (nature.com)
- The brain's extracellular matrix (ECM) mediates structural stability by enwrapping synaptic contacts fundamental for long-term memory storage. (pnas.org)
Mammalian1
- Today I'd like to talk about several of the extracellular matrices, ECM in mammalian skin. (hstalks.com)
Remodeling7
- In this project we analyze effects of extracellular cues for hypertrophic remodeling and examine transcription factor circuits regulating metabolic reprogramming. (uni-heidelberg.de)
- These structural elements form from the same mesodermal primordium, develop in similar ways, have a core leather-like matrix of collagen, and are as responsive - subject to Wolffe's Law of remodeling - to sustained forces going through the body as is the rest of the fascia. (anatomytrains.com)
- Extracellular matrix remodeling and its contribution to protective adaptation following lengthening contractions in human muscle. (umassmed.edu)
- 9 Matrix remodeling may therefore promote an activated EC phenotype in these regions. (ahajournals.org)
- Background- Remodeling of the extracellular matrix (ECM) is a key aspect of myocardial response to biomechanical stress and heart failure. (ahajournals.org)
- Objective- To evaluate if biomarkers reflecting left ventricular/vascular extracellular matrix remodeling are associated with cardiovascular disease (CVD) and death in the community. (ahajournals.org)
- Extracellular matrix (ECM) turnover is an integral component of cardiovascular remodeling. (ahajournals.org)
Fibroblasts1
- 1. A method of treating a disease associated with extracellular matrix (ECM) accumulation in a patient, the method comprising administering to the patient a therapeutically effective amount of fibroblasts which express CD36. (freepatentsonline.com)
Structural1
- During brain maturation, the occurrence of the extracellular matrix (ECM) terminates juvenile plasticity by mediating structural stability. (pnas.org)
AMSBIO1
- AMSBIO supplies M ussel A dhesive P rotein based ma trix ( MAPTrix TM ) recombinant extracellular matrix (ECM) that act as biometic mimics for traditional basement membrane extracts. (amsbio.com)
Filaments1
- Employing optical spectroscopy we have performed a comparative study of the dielectric response of extracellular matrix and filaments of electrogenic bacteria Shewanella oneidensis MR-1, cytochrome c, and bovine serum albumin. (springer.com)
Microenvironments1
- With the use of combinatorial libraries, one can explore the effect of numerous know and unknown factors, alone or in complex combinations in analogous to those encountered in natural extracellular microenvironments. (amsbio.com)
Springer1
- He is the editor of the recent successful book: "Extracellular Composite Matrices in Arthropods" published by Springer in 2016. (springer.com)
Receptor1
- In this study, we address the possibility that CD147 could be a receptor for itself, and we identify the key extracellular domain required for CD147-dependent binding. (aacrjournals.org)