A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
A family of zinc-dependent metalloendopeptidases that is involved in the degradation of EXTRACELLULAR MATRIX components.
A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.
Large, noncollagenous glycoprotein with antigenic properties. It is localized in the basement membrane lamina lucida and functions to bind epithelial cells to the basement membrane. Evidence suggests that the protein plays a role in tumor invasion.
Hexameric extracellular matrix glycoprotein transiently expressed in many developing organs and often re-expressed in tumors. It is present in the central and peripheral nervous systems as well as in smooth muscle and tendons. (From Kreis & Vale, Guidebook to the Extracellular Matrix and Adhesion Proteins, 1993, p93)
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Adherence of cells to surfaces or to other cells.
Glycoproteins which have a very high polysaccharide content.
A member of the metalloproteinase family of enzymes that is principally responsible for cleaving FIBRILLAR COLLAGEN. It can degrade interstitial collagens, types I, II and III.
Compounds that inhibit the enzyme activity or activation of MATRIX METALLOPROTEINASES.
A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
An extracellular endopeptidase of vertebrate tissues similar to MATRIX METALLOPROTEINASE 1. It digests PROTEOGLYCAN; FIBRONECTIN; COLLAGEN types III, IV, V, and IX, and activates procollagenase. (Enzyme Nomenclature, 1992)
Extracellular substance of bone tissue consisting of COLLAGEN fibers, ground substance, and inorganic crystalline minerals and salts.
The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.
A transmembrane domain-containing matrix metalloproteinase. It is synthesized as an inactive zymogen that is activated by the action of PROPROTEIN CONVERTASES such as FURIN. Matrix metalloproteinase 14 plays a direct role in the cleavage of proteins in the pericellular environment. In addition, it can function indirectly by enzymatically activating the proprotein form of MATRIX METALLOPROTEINASE 15.
A family of secreted protease inhibitory proteins that regulates the activity of SECRETED MATRIX METALLOENDOPEPTIDASES. They play an important role in modulating the proteolysis of EXTRACELLULAR MATRIX, most notably during tissue remodeling and inflammatory processes.
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A darkly stained mat-like EXTRACELLULAR MATRIX (ECM) that separates cell layers, such as EPITHELIUM from ENDOTHELIUM or a layer of CONNECTIVE TISSUE. The ECM layer that supports an overlying EPITHELIUM or ENDOTHELIUM is called basal lamina. Basement membrane (BM) can be formed by the fusion of either two adjacent basal laminae or a basal lamina with an adjacent reticular lamina of connective tissue. BM, composed mainly of TYPE IV COLLAGEN; glycoprotein LAMININ; and PROTEOGLYCAN, provides barriers as well as channels between interacting cell layers.
A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a N-glycosylated protein, molecular weight 28 kD, produced by a vast range of cell types and found in a variety of tissues and body fluids. It has been shown to suppress metastasis and inhibit tumor invasion in vitro.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
Integrin beta-1 chains which are expressed as heterodimers that are noncovalently associated with specific alpha-chains of the CD49 family (CD49a-f). CD29 is expressed on resting and activated leukocytes and is a marker for all of the very late activation antigens on cells. (from: Barclay et al., The Leukocyte Antigen FactsBook, 1993, p164)
A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a 21-kDa nonglycosylated protein found in tissue fluid and is secreted as a complex with progelatinase A by human fibroblast and uncomplexed from alveolar macrophages. An overexpression of TIMP-2 has been shown to inhibit invasive and metastatic activity of tumor cells and decrease tumor growth in vivo.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Enzymes that catalyze the degradation of collagen by acting on the peptide bonds.
A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
A secreted matrix metalloproteinase that plays a physiological role in the degradation of extracellular matrix found in skeletal tissues. It is synthesized as an inactive precursor that is activated by the proteolytic cleavage of its N-terminal propeptide.
Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-.
The smallest member of the MATRIX METALLOPROTEINASES. It plays a role in tumor progression.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
HYALURONAN-containing proteoglycans found in the EXTRACELLULAR MATRIX of a variety of tissues and organs. Several versican isoforms exist due to multiple ALTERNATIVE SPLICING of the versican MESSENGER RNA.
A small leucine-rich proteoglycan that interacts with FIBRILLAR COLLAGENS and modifies the EXTRACELLULAR MATRIX structure of CONNECTIVE TISSUE. Decorin has also been shown to play additional roles in the regulation of cellular responses to GROWTH FACTORS. The protein contains a single glycosaminoglycan chain and is similar in structure to BIGLYCAN.
An endopeptidase that is structurally similar to MATRIX METALLOPROTEINASE 2. It degrades GELATIN types I and V; COLLAGEN TYPE IV; and COLLAGEN TYPE V.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
Matrix metalloproteinases that are associated with the CELL MEMBRANE, either through transmembrane domains or GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORS. Membrane-type matrix metalloproteinases may act within the pericellular environment to influence the process of CELL MIGRATION.
A small leucine-rich proteoglycan found in a variety of tissues including CAPILLARY ENDOTHELIUM; SKELETAL MUSCLE; CARTILAGE; BONE; and TENDONS. The protein contains two glycosaminoglycan chains and is similar in structure to DECORIN.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Established cell cultures that have the potential to propagate indefinitely.
Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.
A blood plasma glycoprotein that mediates cell adhesion and interacts with proteins of the complement, coagulation, and fibrinolytic cascade. (From Segen, Dictionary of Modern Medicine, 1992)
Any pathological condition where fibrous connective tissue invades any organ, usually as a consequence of inflammation or other injury.
A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.
Methods for maintaining or growing CELLS in vitro.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A family of related, adhesive glycoproteins which are synthesized, secreted, and incorporated into the extracellular matrix of a variety of cells, including alpha granules of platelets following thrombin activation and endothelial cells. They interact with a number of BLOOD COAGULATION FACTORS and anticoagulant factors. Five distinct forms have been identified, thrombospondin 1, -2, -3, -4, and cartilage oligomeric matrix protein (COMP). They are involved in cell adhesion, platelet aggregation, cell proliferation, angiogenesis, tumor metastasis, VASCULAR SMOOTH MUSCLE growth, and tissue repair.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Restoration of integrity to traumatized tissue.
Polymorphic cells that form cartilage.
A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Ubiquitous macromolecules associated with the cell surface and extracellular matrix of a wide range of cells of vertebrate and invertebrate tissues. They are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (From Cancer Metastasis Rev 1996; 15(2): 177-86; Hepatology 1996; 24(3): 524-32)
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
A secreted matrix metalloproteinase which is highly expressed by MACROPHAGES where it may play a role in INFLAMMATION and WOUND HEALING.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Non-collagenous, calcium-binding glycoprotein of developing bone. It links collagen to mineral in the bone matrix. In the synonym SPARC glycoprotein, the acronym stands for Secreted Protein, Acidic and Rich in Cysteine.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A subclass of matrix metalloproteinases that are secreted into the pericellular space.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Large HYALURONAN-containing proteoglycans found in articular cartilage (CARTILAGE, ARTICULAR). They form into aggregates that provide tissues with the capacity to resist high compressive and tensile forces.
Ability of neoplasms to infiltrate and actively destroy surrounding tissue.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A family of structurally related collagens that form the characteristic collagen fibril bundles seen in CONNECTIVE TISSUE.
Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.
A fibrillar collagen consisting of three identical alpha1(III) chains that is widely distributed in many tissues containing COLLAGEN TYPE I. It is particularly abundant in BLOOD VESSELS and may play a role in tissues with elastic characteristics.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Elements of limited time intervals, contributing to particular results or situations.
Specific cell surface receptors which bind to FIBRONECTINS. Studies have shown that these receptors function in certain types of adhesive contact as well as playing a major role in matrix assembly. These receptors include the traditional fibronectin receptor, also called INTEGRIN ALPHA5BETA1 and several other integrins.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
PROTEOGLYCANS-associated proteins that are major components of EXTRACELLULAR MATRIX of various tissues including CARTILAGE; and INTERVERTEBRAL DISC structures. They bind COLLAGEN fibers and contain protein domains that enable oligomer formation and interaction with other extracellular matrix proteins such as CARTILAGE OLIGOMERIC MATRIX PROTEIN.
A secreted matrix metalloproteinase that may play a role in matrix degradation during WOUND HEALING. It is expressed at high levels by KERATINOCYTES, suggesting its role in keratinocyte migration.
Proteins prepared by recombinant DNA technology.
An enzyme that catalyzes the random hydrolysis of 1,4-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. (From Enzyme Nomenclature, 1992) There has been use as ANTINEOPLASTIC AGENTS to limit NEOPLASM METASTASIS.
Surface ligands that mediate cell-to-cell adhesion and function in the assembly and interconnection of the vertebrate nervous system. These molecules promote cell adhesion via a homophilic mechanism. These are not to be confused with NEURAL CELL ADHESION MOLECULES, now known to be expressed in a variety of tissues and cell types in addition to nervous tissue.
A non-fibrillar collagen that forms a network of MICROFIBRILS within the EXTRACELLULAR MATRIX of CONNECTIVE TISSUE. The alpha subunits of collagen type VI assemble into antiparallel, overlapping dimers which then align to form tetramers.
Dystrophin-associated proteins that play role in the formation of a transmembrane link between laminin-2 and DYSTROPHIN. Both the alpha and the beta subtypes of dystroglycan originate via POST-TRANSLATIONAL PROTEIN PROCESSING of a single precursor protein.
An anchoring junction of the cell to a non-cellular substrate. It is composed of a specialized area of the plasma membrane where bundles of the ACTIN CYTOSKELETON terminate and attach to the transmembrane linkers, INTEGRINS, which in turn attach through their extracellular domains to EXTRACELLULAR MATRIX PROTEINS.
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Specialized areas at the CELL MEMBRANE where a cell attaches to the EXTRACELLULAR MATRIX or other substratum.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A cell line derived from cultured tumor cells.
Peptides composed of between two and twelve amino acids.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A family of secreted multidomain proteins that were originally identified by their association with the latent form of TRANSFORMING GROWTH FACTORS. They interact with a variety of EXTRACELLULAR MATRIX PROTEINS and may play a role in the regulation of TGB-beta bioavailability.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
The process by which cells convert mechanical stimuli into a chemical response. It can occur in both cells specialized for sensing mechanical cues such as MECHANORECEPTORS, and in parenchymal cells whose primary function is not mechanosensory.
The quality of surface form or outline of CELLS.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Major component of chondrocyte EXTRACELLULAR MATRIX of various tissues including bone, tendon, ligament, SYNOVIUM and blood vessels. It binds MATRILIN PROTEINS and is associated with development of cartilage and bone.
A member of the family of tissue inhibitor of metalloproteinases. Mutations of the gene for TIMP3 PROTEIN causes Sorsby fundus dystrophy.
A family of non-receptor, PROLINE-rich protein-tyrosine kinases.
A layer of vascularized connective tissue underneath the EPIDERMIS. The surface of the dermis contains innervated papillae. Embedded in or beneath the dermis are SWEAT GLANDS; HAIR FOLLICLES; and SEBACEOUS GLANDS.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It is found in hypertrophic CHONDROCYTES where it may play a role in CHONDROGENESIS and endochondral ossification.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Specialized structures of the cell that extend the cell membrane and project out from the cell surface.
An enzyme oxidizing peptidyl-lysyl-peptide in the presence of water & molecular oxygen to yield peptidyl-allysyl-peptide plus ammonia & hydrogen peroxide. EC
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
A biosynthetic precursor of collagen containing additional amino acid sequences at the amino-terminal and carboxyl-terminal ends of the polypeptide chains.
The thin membranous structure supporting the adjoining glomerular capillaries. It is composed of GLOMERULAR MESANGIAL CELLS and their EXTRACELLULAR MATRIX.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
An integrin found in FIBROBLASTS; PLATELETS; MONOCYTES, and LYMPHOCYTES. Integrin alpha5beta1 is the classical receptor for FIBRONECTIN, but it also functions as a receptor for LAMININ and several other EXTRACELLULAR MATRIX PROTEINS.
A BRAIN-specific hyalectin that may play a role in terminally differentiating NEURONS. It is found highly overexpressed in primary BRAIN TUMORS and in experimental models of GLIOMA.
A proteolytic enzyme that converts PLASMINOGEN to FIBRINOLYSIN where the preferential cleavage is between ARGININE and VALINE. It was isolated originally from human URINE, but is found in most tissues of most VERTEBRATES.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
A secreted matrix metalloproteinase that is believed to play a role in EXTRACELLULAR MATRIX remodeling and cell fate determination during normal and pathological processes. Matrix metalloproteinase 11 was originally isolated in primary BREAST NEOPLASMS and may be involved in the process of tumorigenesis.
Spindle-shaped cells with characteristic CONTRACTILE PROTEINS and structures that contribute to the WOUND HEALING process. They occur in GRANULATION TISSUE and also in pathological processes such as FIBROSIS.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Connective tissue comprised chiefly of elastic fibers. Elastic fibers have two components: ELASTIN and MICROFIBRILS.
A fibrillar collagen found predominantly in CARTILAGE and vitreous humor. It consists of three identical alpha1(II) chains.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Numerical expression indicating the measure of stiffness in a material. It is defined by the ratio of stress in a unit area of substance to the resulting deformation (strain). This allows the behavior of a material under load (such as bone) to be calculated.
A non-receptor protein tyrosine kinase that is localized to FOCAL ADHESIONS and is a central component of integrin-mediated SIGNAL TRANSDUCTION PATHWAYS. Focal adhesion kinase 1 interacts with PAXILLIN and undergoes PHOSPHORYLATION in response to adhesion of cell surface integrins to the EXTRACELLULAR MATRIX. Phosphorylated p125FAK protein binds to a variety of SH2 DOMAIN and SH3 DOMAIN containing proteins and helps regulate CELL ADHESION and CELL MIGRATION.
The nonstriated involuntary muscle tissue of blood vessels.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Proteases which use a metal, normally ZINC, in the catalytic mechanism. This group of enzymes is inactivated by metal CHELATORS.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Materials fabricated by BIOMIMETICS techniques, i.e., based on natural processes found in biological systems.
A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
This integrin alpha subunit combines with INTEGRIN BETA1 to form a receptor (INTEGRIN ALPHA5BETA1) that binds FIBRONECTIN and LAMININ. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds.
Glycoprotein molecules on the surface of cells that react with or bind to laminin whose function allows the binding of epithelial cells to the basement membrane. The molecular weight of this high-affinity receptor is 67 kD.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
The process of bone formation. Histogenesis of bone including ossification.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Single preparations containing two or more active agents, for the purpose of their concurrent administration as a fixed dose mixture.
Receptors such as INTEGRIN ALPHAVBETA3 that bind VITRONECTIN with high affinity and play a role in cell migration. They also bind FIBRINOGEN; VON WILLEBRAND FACTOR; osteopontin; and THROMBOSPONDINS.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Signal molecules that are involved in the control of cell growth and differentiation.
The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube.
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
The properties, processes, and behavior of biological systems under the action of mechanical forces.
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Proteins associated with the inner surface of the lipid bilayer of the viral envelope. These proteins have been implicated in control of viral transcription and may possibly serve as the "glue" that binds the nucleocapsid to the appropriate membrane site during viral budding from the host cell.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
The physiological renewal, repair, or replacement of tissue.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Resistance and recovery from distortion of shape.
A porelike structure surrounding the entire circumference of the anterior chamber through which aqueous humor circulates to the canal of Schlemm.
APOPTOSIS triggered by loss of contact with the EXTRACELLULAR MATRIX.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Antibodies produced by a single clone of cells.
An extracellular matrix glycoprotein from platelets and a variety of normal and transformed cells of both mesenchymal and epithelial origin. Thrombospondin-1 is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. Also, it has been studied for its use as a potential regulator of tumor growth and metastasis.
The main trunk of the systemic arteries.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Polymeric materials (usually organic) of large molecular weight which can be shaped by flow. Plastic usually refers to the final product with fillers, plasticizers, pigments, and stabilizers included (versus the resin, the homogeneous polymeric starting material). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An alpha integrin with a molecular weight of 160-kDa that is found in a variety of cell types. It undergoes posttranslational cleavage into a heavy and a light chain that are connected by disulfide bonds. Integrin alphaV can combine with several different beta subunits to form heterodimers that generally bind to RGD sequence-containing extracellular matrix proteins.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
The rate dynamics in chemical or physical systems.
Interstitial space between cells, occupied by INTERSTITIAL FLUID as well as amorphous and fibrous substances. For organisms with a CELL WALL, the extracellular space includes everything outside of the CELL MEMBRANE including the PERIPLASM and the cell wall.

Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins. (1/9343)

Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether Bcr-Abl assembles multi-protein complexes and if it does where these complexes are within cells. We have investigated the localization of Bcr-Abl in 32D myeloid cells attached to the extracellular matrix. We have found that Bcr-Abl displays a polarized distribution, colocalizing with a subset of filamentous actin at trailing portions of migrating 32D cells, and localizes on the cortical F-actin and on vesicle-like structures in resting 32D cells. Deletion of the actin binding domain of Bcr-Abl (Bcr-AbI-AD) dramatically enhances the localization of Bcr-Abl on the vesicle-like structures. These distinct localization patterns of Bcr-Abl and Bcr-Abl-AD enabled us to examine the localization of Bcr-Abl substrate and interacting proteins in relation to Bcr-Abl. We found that a subset of biochemically defined target proteins of Bcr-Abl redistributed and co-localized with Bcr-Abl on F-actin and on vesicle-like structures. The co-localization of signaling proteins with Bcr-Abl at its sites of localization supports the idea that Bcr-Abl forms a multi-protein signaling complex, while the polarized distribution and vesicle-like localization of Bcr-Abl may play a role in leukemogenesis.  (+info)

Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2. (2/9343)

Interaction between integrin alphavbeta3 and extracellular matrix is crucial for endothelial cells sprouting from capillaries and for angiogenesis. Furthermore, integrin-mediated outside-in signals co-operate with growth factor receptors to promote cell proliferation and motility. To determine a potential regulation of angiogenic inducer receptors by the integrin system, we investigated the interaction between alphavbeta3 integrin and tyrosine kinase vascular endothelial growth factor receptor-2 (VEGFR-2) in human endothelial cells. We report that tyrosine-phosphorylated VEGFR-2 co-immunoprecipitated with beta3 integrin subunit, but not with beta1 or beta5, from cells stimulated with VEGF-A165. VEGFR-2 phosphorylation and mitogenicity induced by VEGF-A165 were enhanced in cells plated on the alphavbeta3 ligand, vitronectin, compared with cells plated on the alpha5beta1 ligand, fibronectin or the alpha2beta1 ligand, collagen. BV4 anti-beta3 integrin mAb, which does not interfere with endothelial cell adhesion to vitronectin, reduced (i) the tyrosine phosphorylation of VEGFR-2; (ii) the activation of downstream transductor phosphoinositide 3-OH kinase; and (iii) biological effects triggered by VEGF-A165. These results indicate a new role for alphavbeta3 integrin in the activation of an in vitro angiogenic program in endothelial cells. Besides being the most important survival system for nascent vessels by regulating cell adhesion to matrix, alphavbeta3 integrin participates in the full activation of VEGFR-2 triggered by VEGF-A, which is an important angiogenic inducer in tumors, inflammation and tissue regeneration.  (+info)

Inhibition of transforming growth factor beta production by nitric oxide-treated chondrocytes: implications for matrix synthesis. (3/9343)

OBJECTIVE: Nitric oxide (NO) is generated copiously by articular chondrocytes activated by interleukin-1beta (IL-1beta). If NO production is blocked, much of the IL-1beta inhibition of proteoglycan synthesis is prevented. We tested the hypothesis that this inhibitory effect of NO on proteoglycan synthesis is secondary to changes in chondrocyte transforming growth factor beta (TGFbeta). METHODS: Monolayer, primary cultures of lapine articular chondrocytes and cartilage slices were studied. NO production was determined as nitrite accumulation in the medium. TGFbeta bioactivity in chondrocyte- and cartilage-conditioned medium (CM) was measured with the mink lung epithelial cell bioassay. Proteoglycan synthesis was measured as the incorporation of 35S-sodium sulfate into macromolecules separated from unincorporated label by gel filtration on PD-10 columns. RESULTS: IL-1beta increased active TGFbeta in chondrocyte CM by 12 hours; by 24 hours, significant increases in both active and latent TGFbeta were detectable. NG-monomethyl-L-arginine (L-NMA) potentiated the increase in total TGFbeta without affecting the early TGFbeta activation. IL-1beta stimulated a NO-independent, transient increase in TGFbeta3 at 24 hours; however, TGFbeta1 was not changed. When NO synthesis was inhibited with L-NMA, IL-1beta increased CM concentrations of TGFbeta1 from 24-72 hours of culture. L-arginine (10 mM) reversed the inhibitory effect of L-NMA on NO production and blocked the increases in TGFbeta1. Anti-TGFbeta1 antibody prevented the restoration of proteoglycan synthesis by chondrocytes exposed to IL-1beta + L-NMA, confirming that NO inhibition of TGFbeta1 in IL-1beta-treated chondrocytes effected, in part, the decreased proteoglycan synthesis. Furthermore, the increase in TGFbeta and proteoglycan synthesis seen with L-NMA was reversed by the NO donor S-nitroso-N-acetylpenicillamide. Similar results were seen with cartilage slices in organ culture. The autocrine increase in CM TGFbeta1 levels following prior exposure to TGFbeta1 was also blocked by NO. CONCLUSION: NO can modulate proteoglycan synthesis indirectly by decreasing the production of TGFbeta1 by chondrocytes exposed to IL-1beta. It prevents autocrine-stimulated increases in TGFbeta1, thus potentially diminishing the anabolic effects of this cytokine in chondrocytes.  (+info)

Role of thrombin receptor in breast cancer invasiveness. (4/9343)

Invasion, the ability of an epithelial cancer cell to detach from and move through a basement membrane, is a central process in tumour metastasis. Two components of invasion are proteolysis of extracellular matrix and cellular movement through it. A potential promoter of these two processes is thrombin, the serine proteinase derived from the ubiquitous plasma protein prothrombin. Thrombin promotes the invasion of MDA-MB231 breast tumour cells (a highly aggressive cell line) in an in vitro assay. Invasion by MDA-MB436 and MCF-7 cells, less aggressive cell lines, is not promoted by thrombin. Thrombin, added to the cells, is a stimulator of cellular movement; fibroblast-conditioned medium is the chemotaxin. Thrombin-promoted invasion is inhibited by hirudin. Stimulation of invasion is a receptor-mediated process that is mimicked by a thrombin receptor-activating peptide. Thrombin has no effect on chemotaxis in vitro. Thrombin receptor is detectable on the surface of MDA-MB231 cells, but not on the other two cell lines. Introduction of oestrogen receptors into MDA-MB231 cells by transfection with pHEO had no effect on thrombin receptor expression, in the presence or absence of oestradiol. This paper demonstrates that thrombin increases invasion by the aggressive breast cancer cell line MDA-MB231 by a thrombin receptor-dependent mechanism.  (+info)

Extracellular matrix remodelling in the endometrium and its possible relevance to the pathogenesis of endometriosis. (5/9343)

Essential features of endometrial physiology involve the extracellular matrix (ECM). In the pathogenesis of endometriosis, interactions of endometriosis cells with ECM can be postulated. Two systems of secreted proteases in the endometrium, the plasmin(ogen) activator/inhibitor and the matrix metalloproteinases and their inhibitors were examined in cell cultures of uterine endometrial cells from women with and without endometriosis. Soluble urokinase receptor secretion is increased, and mRNA transcription of tissue inhibitor of metalloproteinases-2 (TIMP-2) is upregulated by progestin in endometriosis. These findings are compatible with an altered ECM turnover in the endometrium of these patients that may explain a higher invasive potential of retrogradely menstruated endometrial fragments.  (+info)

Mechanisms and mediators in coal dust induced toxicity: a review. (6/9343)

Chronic inhalation of coal dust can cause several lung disorders, including simple coal workers pneumoconiosis (CWP), progressive massive fibrosis (PMF), chronic bronchitis, lung function loss, and emphysema. This review focuses on the cellular actions and interactions of key inflammatory cells and target cells in coal dust toxicity and related lung disorders, i.e. macrophages and neutrophils, epithelial cells, and fibroblasts. Factors released from or affecting these cells are outlined in separate sections, i.e. (1) reactive oxygen species (ROS) and related antioxidant protection mechanisms, and (2) cytokines, growth factors and related proteins. Furthermore, (3) components of the extracellular matrix (ECM), including the modifying role of ROS, cytokines, proteases and antiproteases are discussed in relation to tissue damage and remodelling in the respiratory tract. It is recognised that inhaled coal dust particles are important non-cellular and cellular sources of ROS in the lung, and may be significantly involved in the damage of lung target cells as well as important macromolecules including alpha-1-antitrypsin and DNA. In vitro and in vivo studies with coal dusts showed the up-regulation of important leukocyte recruiting factors, e.g. Leukotriene-B4 (LTB4), Platelet Derived Growth Factor (PDGF), Monocyte Chemotactic Protein-1 (MCP-1), and Tumor Necrosis Factor-alpha (TNF alpha), as well as the neutrophil adhesion factor Intercellular Adhesion Molecule-1 (ICAM-1). Coal dust particles are also known to stimulate the (macrophage) production of various factors with potential capacity to modulate lung cells and/or extracellular matrix, including O2-., H2O2, and NO, fibroblast chemoattractants (e.g. Transforming Growth Factor-beta (TGF beta), PDGF, and fibronectin) and a number of factors that have been shown to stimulate and/or inhibit fibroblast growth or collagen production such as (TNF alpha, TGF beta, PDGF, Insulin Like Growth Factor, and Prostaglandin-E2). Further studies are needed to clarify the in vivo kinetics and relative impact of these factors.  (+info)

Matrix valency regulates integrin-mediated lymphoid adhesion via Syk kinase. (7/9343)

Lymphocytes accumulate within the extracellular matrix (ECM) of tumor, wound, or inflammatory tissues. These tissues are largely comprised of polymerized adhesion proteins such as fibrin and fibronectin or their fragments. Nonactivated lymphoid cells attach preferentially to polymerized ECM proteins yet are unable to attach to monomeric forms or fragments of these proteins without previous activation. This adhesion event depends on the appropriate spacing of integrin adhesion sites. Adhesion of nonactivated lymphoid cells to polymeric ECM components results in activation of the antigen receptor-associated Syk kinase that accumulates in adhesion-promoting podosomes. In fact, activation of Syk by antigen or agonists, as well as expression of an activated Syk mutant in lymphoid cells, facilitates their adhesion to monomeric ECM proteins or their fragments. These results reveal a cooperative interaction between signals emanating from integrins and antigen receptors that can serve to regulate stable lymphoid cell adhesion and retention within a remodeling ECM.  (+info)

alphaSU2, an epithelial integrin that binds laminin in the sea urchin embryo. (8/9343)

At gastrulation in the sea urchin embryo dramatic cell adhesion changes contribute to primary mesenchyme cell ingression movements and to cell rearrangements during archenteron invagination. At ingression, quantitative adhesion assays demonstrated previously that primary mesenchyme cells (PMCs) change their affinity for neighboring cells, for a fibronectin-like substrate, and for the hyaline layer. To investigate the molecular basis for these and other differential cell affinities at gastrulation, we have identified an integrin that appears to be responsible for specific alterations in cell-substrate adhesion to laminin. During early cleavage stages blastomeres adhere poorly to laminin substrates. Around hatching there is a large increase in the ability of blastomeres to bind to laminin and this increase correlates temporally with the expression of an integrin on the basal surface all blastomeres. PMCs, after undergoing their epithelial-mesenchymal transition, have a strongly reduced affinity for laminin relative to ectoderm cells and, correspondingly, do not stain for the presence of the integrin. We identified the alpha integrin cDNA from Lytechinus variegatus by RT-PCR. Overlapping clones were obtained from a midgastrula cDNA library to provide a complete sequence for the integrin. The composite cDNA encoded a protein that was most similar to the alpha5 subgroup of vertebrate integrins, but there was not a definitive vertebrate integrin homolog. Northern blots and Western immunoblots showed that the sea urchin integrin, which we have named alphaSU2, is present in eggs and during all stages of development. Immunolocalization with specific polyclonal antibodies showed that alphaSU2 first appears on the basal cell surface of epithelia at the midblastula stage, at a time correlating with the increase in adhesive affinity for laminin. The protein remains at high levels on the basal surface of ectoderm cells but is temporarily reduced or eliminated from endoderm cells during their convergent-extension movements. To confirm integrin binding specificity, alphaSU2 was transfected into an alpha-integrin-deficient CHO cell line. alphaSU2-expressing CHO cells bound well to isolated sea urchin basal lamina and to purified laminin. The transfected cells bound weakly or not at all to fibronectin, type I collagen, and type IV collagen. This is consistent with the hypothesis that alphaSU2 integrin functions by binding epithelial cells to laminin in the basal lamina. In vivo, modulation of alphaSU2 integrin expression correlates with critical adhesive changes during cleavage and gastrulation. Thus, this protein appears to be an important contributor to the morphogenetic rearrangements that characterize gastrulation in the sea urchin embryo.  (+info)

TY - JOUR. T1 - Extracellular matrix organization modulates fibroblast growth and growth factor responsiveness. AU - Nakagawa, Shigenori. AU - Pawelek, Pamela. AU - Grinnell, Frederick. N1 - Funding Information: We are grateful to Drs. William Snell and George Bloom for their advice and suggestions.T his research was supported by grants from the Kendall Health Care Products Co. and the NIH (DM31321).. PY - 1989/6. Y1 - 1989/6. N2 - To learn more about the relationship between extracellular matrix organization, cell shape, and cell growth control, we studied DNA synthesis by fibroblasts in collagen gels that were either attached to culture dishes or floating in culture medium during gel contraction. After 4 days of contraction, the collagen density (initially 1.5 mg/ml) reached 22 mg/ml in attached gels and 55 mg/ml in floating gels. After contraction, attached collagen gels were well organized; collagen fibrils were aligned in the plane of cell spreading; and fibroblasts had an elongated, ...
TY - JOUR. T1 - Breast cancer cell cyclooxygenase-2 expression alters extracellular matrix structure and function and numbers of cancer associated fibroblasts. AU - Krishnamachary,Balaji. AU - Stasinopoulos,Ioannis. AU - Kakkad,Samata. AU - Penet,Marie France. AU - Jacob,Desmond. AU - Wildes,Flonne. AU - Mironchik,Yelena. AU - Pathak,Arvind P.. AU - Solaiyappan,Meiyappan. AU - Bhujwalla,Zaver M.. PY - 2017. Y1 - 2017. N2 - Cyclooxygenase-2 (COX-2) is a critically important mediator of inflammation that significantly influences tumor angiogenesis, invasion, and metastasis. We investigated the role of COX-2 expressed by triple negative breast cancer cells in altering the structure and function of the extracellular matrix (ECM). COX-2 downregulation effects on ECM structure and function were investigated using magnetic resonance imaging (MRI) and second harmonic generation (SHG) microscopy of tumors derived from triple negative MDA-MB-231 breast cancer cells, and a derived clone stably expressing a ...
Here we describe extracellular matrix alterations in footpad lesions and draining lymph nodes caused by Leishmania (L.) amazonensis in mouse strains with distinct susceptibilities to this parasite: BALB/c (susceptible), C57BL/6 (intermediate), and DBA/2 (resistant). Changes in ECM were observed mainly in BALB/c mice that, in general, presented tissue damage associated with high parasite burden. Under polarized light, Sirius Red revealed type I collagen that was predominant in the primary lesion in all strains studied at the early phase of infection, but gradually decreased and was replaced by abundant type III collagen fibre in chronic phase lesions. the presence of type III collagen seemed to provide support to inflammatory cells, mainly vacuolated and parasitized macrophages. Laminin expression was not altered during infection by L. (L.) amazonensis in any of the mouse strains studied. Furthermore, the decreased fibronectin expression, in all strains, in areas where amastigotes have been ...
Antibodies for proteins involved in regulation of extracellular matrix organization pathways, according to their Panther/Gene Ontology Classification
Antibodies for proteins involved in extracellular matrix organization pathways, according to their Panther/Gene Ontology Classification
Airway remodelling describes the histopathological changes leading to fixed airway obstruction in patients with asthma and includes extra-cellular matrix (ECM) deposition. Matrix metalloproteinase-1 (MMP-1) is present in remodelled airways but its relationship with ECM proteins and the resulting functional consequences are unknown. We used airway smooth muscle cells (ASM) and bronchial biopsies from control donors and patients with asthma to examine the regulation of MMP-1 by ECM in ASM cells and the effect of MMP-1 on ASM contraction. Collagen-I and tenascin-C induced MMP-1 protein expression, which for tenascin-C, was greater in asthma derived ASM cells. Tenascin-C induced MMP-1 expression was dependent on ERK1/2, JNK and p38 MAPK activation and attenuated by function blocking antibodies against the β1 and β3 integrin subunits. Tenascin-C and MMP-1 were not expressed in normal airways but co-localised in the ASM bundles and reticular basement membrane of patients with asthma. Further, ECM ...
This chapter introduces the matrix-mechanics formulation of quantum mechanics, emphasizing both calculational techniques and conceptual understanding. Parallels between matrix mechanics and ordinary vectors and matrices are extensively utilized. Starting with the representation of ordinary vectors as rows or columns of numbers, the scalar product is discussed, followed by the transformation of vectors by matrices, as illustrated by rotations. The vector representation of quantumstates, the inner product of two such states, and the matrix representation of operators are then introduced. The simple forms assumed in matrix mechanics by a basis state, and by an operator, when either is written in its eigenbasis, are discussed, as are the specific forms of adjoint, Hermitian, and unitary operators. The chapter concludes with a brief exposition of eigenvalue equations in matrix mechanics.
Read how fascia and extra-cellular matrix (ECM) are crucial for stability and movement. Learn to define the fascial system and ECM.
Perturbation of mechanical force at muscle attachments and its effects on tendon morphogenesis provides insights into the mechanisms underlying cellular responses to tensional force and resulting extracellular matrix production.
View Notes - BIO 320 Lec18.2009.notes from BIO 50160 at University of Texas. Lecture 18 Extracellular Matrix: Interactions between Cells and Their Environment Figure 19-3 Molecular Biology of the
Insulin-like growth factor (IGF)-I binds to the ECM protein vitronectin (VN) through IGF binding proteins (IGFBPs) to enhance proliferation and migration of skin keratinocytes and fibroblasts. Although evidence exists for the role of individual components of the complex (IGF-I, IGFBP-3 and VN), the cellular functions stimulated by these proteins together as a complex remains un-investigated in melanoma cells. We report here that the IGF-I:IGFBP-3:VN trimeric complex stimulates a dose-dependent increase in the proliferation and migration of WM35 and Sk-MEL28 melanoma cells. In 3D Matrigel™ and hydrogel cultures, both cell lines formed primary tumor-like spheroids, which increased in size in a dose-dependent manner in response to the trimeric complex. Furthermore, we reveal IGFBP-3:VN protein complexes in malignant melanoma and squamous cell carcinoma patient tissues, where the IGFBP-3:VN complex was seen to be predominantly tumor cell-associated. Peptide antagonists designed to target the ...
N-acetyl proline-glycine-proline (ac-PGP) is a matrix-derived chemokine produced through the proteolytic destruction of collagen by matrix metalloproteinases (MMPs). While upregulation and activation of MMPs and concomitant degradation of the extracellular matrix are known to be associated with neurological injury in ischemic stroke, the production of ac-PGP in stroke brain and its effects on neurons have not been investigated. We examined the effects of ac-PGP on primary cortical neurons and found that it binds neuronal CXCR2 receptors, activates extracellular signal-regulated kinase 1/2 (ERK1/2), and induces apoptosis associated with caspase-3 cleavage in a dose-dependent manner. After transient ischemic stroke in rats, ac-PGP was significantly upregulated in infarcted brain tissue. The production of ac-PGP in brain in ischemia/reperfusion injury and its propensity to induce apoptosis in neurons may link MMP-mediated destruction of the extracellular matrix and opening of the blood-brain ...
Post-Doctoral Fellow, *Faculty of Dentistry, University of Toronto, *Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, Toronto, *Department of Laboratory Medicine & Pathobiology, Faculty of Medicine, University of Toronto ...
We recently showed that differential expression of extracellular matrix (ECM) genes delineates four subgroups of breast carcinomas (ECM1, -2, -3- and -4) with different clinical outcome. To further investigate the characteristics of ECM signature and its impact on tumor progression, we conducted unsupervised clustering analyses in 6 additional independent datasets of invasive breast tumors from different platforms for a total of 643 samples. Use of four different clustering algorithms identified ECM3 tumors as an independent group in all datasets tested. ECM3 showed a homogeneous gene pattern, consisting of 58 genes encoding 43 structural ECM proteins. From 26 to 41% of the cases were ECM3-enriched, and analysis of datasets relevant to gene expression in neoplastic or corresponding stromal cells showed that both stromal and breast carcinoma cells can coordinately express ECM3 genes. In in vitro experiments, β-estradiol induced ECM3 gene production in ER-positive breast carcinoma cell lines, ...
Purpose: : We previously found that IGF-I, TGF-β, and PDGF, but not FGF-2, stimulate collagen synthesis by keratocytes in culture. We also found that culturing insulin activated keratocytes under a thin layer of agarose increases the processing of procollagen to collagen and increases ECM formation (PMID: 18938157). We now evaluate the ECM formed by keratocytes cultured in these growth factors and under agarose. Methods: : Collagenase-isolated keratocytes from bovine corneas were plated at 40,000 cells/cm2 and then cultured with DMEM/F12 alone, or DMEM/F12 supplemented with either 10ng IGF-I, 2ng TGF-β, 10ng FGF-2, or 10ng PDGF/ml, all with ascorbate. Cultures were overlayed with ~1mm of 3% agarose on day 4 and harvested for analysis on day 12. Keratocytes cell number was determined by measuring DNA content (cyquant assay). Collagen was determined by pepsin digestion, SDS/PAGE, simply blue staining, and by western blots with antibodies to procollagen I and III. ECM morphology was evaluated by ...
Extracellular matrices are essential for cellular and organismal function. Recent genome-wide and phenome-wide association studies started to reveal a broad spectrum of phenotypes associated with genetic variants. However, the phenome or spectrum of all phenotypes associated with genetic variants in extracellular matrix genes is unknown. Here, we analyzed over two million recorded genotype-to-phenotype relationships across multiple species to define their extracellular matrix phenomes. By using the previously defined matrisomes of humans, mice, zebrafish, Drosophila, and C. elegans, we found that the extracellular matrix phenome comprises of 3-10% of the entire phenome. Collagens (COL1A1, COL2A1) and fibrillin (FBN1) are each associated with ,150 distinct phenotypes in humans, whereas collagen COL4A1, Wnt- and sonic hedgehog (shh) signaling are predominantly associated in other species. We determined the phenotypic fingerprints of matrisome genes and found that MSTN, CTSD, LAMB2, HSPG2, and ...
Introduction: Changes in ventricular extracellular matrix (ECM) composition of hypertrophic cardiomyopathy determine clinical outcomes. The effects of MSC transplantation upon ventricular remodeling and determinants of ECM composition in hypertrophic cardiomyopathy have not been studied.. Hypothesis: We hypothesized that MSC therapy has beneficial effects upon ventricular remodeling and ECM proteases and tissue inhibitors in a rat model of pressure overload cardiomyopathy.. Methods: Sprague-Dawley rats underwent aortic banding and were followed by echocardiography for development of heart failure. After a decrease in fractional shortening of 25% from baseline, intra-coronary randomized injection of 1 x 106 MSC (n=28) or PBS (n=20) was performed. Serial echocardiography was performed to identify reverse remodeling. Left ventricular protein analysis including matrix metalloproteinases (MMP-2, 3, 6 and 9) and tissue inhibitors of metalloproteinases (TIMP-1, 2 and 3) was performed after sacrifice on ...
Limitations associated with demineralised bone matrix and other grafting materials have motivated the development of alternative strategies to enhance the repair of large bone defects. The growth plate (GP) of developing limbs contain a plethora of growth factors and matrix cues which contribute to long bone growth, suggesting that biomaterials derived from its extracellular matrix (ECM) may be uniquely suited to promoting bone regeneration. The goal of this study was to generate porous scaffolds from decellularised GP ECM and to evaluate their ability to enhance host mediated bone regeneration following their implantation into critically-sized rat cranial defects. The scaffolds were first assessed by culturing with primary human macrophages, which demonstrated that decellularisation resulted in reduced IL-1β and IL-8 production. In vitro, GP derived scaffolds were found capable of supporting osteogenesis of mesenchymal stem cells via either an intramembranous or an endochondral pathway, demonstrating
TY - JOUR. T1 - Cardiac extracellular matrix remodeling: Fibrillar collagens and Secreted Protein Acidic and Rich in Cysteine (SPARC). AU - McCurdy, Sarah. AU - Baicu, Catalin F.. AU - Heymans, Stephane. AU - Bradshaw, Amy D.. PY - 2010/3. Y1 - 2010/3. KW - BM-40. KW - Osteonectin. KW - SPARC. KW - Extracellular matrix. KW - Remodeling. KW - Review. U2 - 10.1016/j.yjmcc.2009.06.018. DO - 10.1016/j.yjmcc.2009.06.018. M3 - Article. C2 - 19577572. VL - 48. SP - 544. EP - 549. JO - Journal of Molecular and Cellular Cardiology. JF - Journal of Molecular and Cellular Cardiology. SN - 0022-2828. IS - 3. ER - ...
Cognitive impairment associated with MDD has been well characterized (33-35). This includes deficits in declarative and spatial memory (36, 37), supporting a role for hippocampus-mediated dysfunction and other related (endo)phenotypes, for example, decreased hippocampal volume, in MDD (38). However, the molecular mechanisms underlying this association remain to be elucidated. Here, we used a preclinical rat model that induces several long-lasting depressive-like behaviors (11, 12) to investigate the connection between hippocampal pathology and cognitive deficits. Our data indicate a causal relationship between aberrant synaptic CSPG expression, alterations in the number of PNNs, and dysregulation of the hippocampal network that, together, mediate cognitive impairments in our rat model.. Collectively, our data highlight the dorsal hippocampus as a principal mediator of cognitive deficits in the SDPS paradigm. At the behavioral level, SDPS impaired short-term object location memory, as assessed by ...
TY - JOUR. T1 - Differential matrix rigidity response in breast cancer cell lines correlates with the tissue tropism. AU - Kostic, Ana. AU - Lynch, Christopher D.. AU - Sheetz, Michael. PY - 2009/7/23. Y1 - 2009/7/23. N2 - Metastasis to a variety of distant organs, such as lung, brain, bone, and liver, is a leading cause of mortality in the breast cancer patients. The tissue tropism of breast cancer metastasis has been recognized and studied extensively, but the cellular processes underlying this phenomenon, remain elusive. Modern technologies have enabled the discovery of a number of the genetic factors determining tissue tropism of malignant cells. However, the effect of these genetic differences on the cell motility and invasiveness is poorly understood. Here, we report that cellular responses to the mechanical rigidity of the extracellular matrix correlate with the rigidity of the target tissue. We tested a series of single cell populations isolated from MDA-MB-231 breast cancer cell line in ...
The purpose of this study was to determine whether extracellular matrix (ECM) composition through integrin receptors modulated the volume-sensitive osmolyte anion channels (VSOACs) in skeletal muscle-derived C2C12 cells. Cl? currents in cells plated on FN. In summary, ECM composition and integrins impact the biophysical properties and systems of onset of VSOACs profoundly. = can be the entire cell capacitance MK-2206 2HCl (in pF), can be the quantity of charge moved (in fC), and can be the degree of the voltage-clamp stage. The E+-free of charge pipette and exterior solutions had been designed to reduce the activity of endogenous E+ stations by including cesium in the inner remedy, and tetraethylammonium chloride (TEA) and barium chloride in the superfusate. The exterior hypotonic and isotonic solutions had been produced using a common foundation remedy, which included (in millimeter) 90 NaCl; 0.66 MgCl2; 1 CaCl2; 2 BaCl2; 10 TEA-Cl; 10 HEPES; and 5.5 glucose; pH was modified to 7.4 with NaOH. ...
Fibrotic cardiac disease, a leading cause of death worldwide, manifests as substantial loss of function following maladaptive tissue remodeling. Fibrosis can affect both the heart valves and the myocardium and is characterized by the activation of fibroblasts and accumulation of extracellular matrix. Valvular interstitial cells and cardiac fibroblasts, the cell types responsible for maintenance of cardiac extracellular matrix, are sensitive to changing mechanical environments, and their ability to sense and respond to mechanical forces determines both normal development and the progression of disease. Recent studies have uncovered specific adhesion proteins and mechano-sensitive signaling pathways that contribute to the progression of fibrosis. Integrins form adhesions with the extracellular matrix, and respond to changes in substrate stiffness and extracellular matrix composition. Cadherins mechanically link neighboring cells and are likely to contribute to fibrotic disease propagation. ...
Cells Expressing CD44 Antigen (CDw44 or Epican or Extracellular Matrix Receptor III or GP90 Lymphocyte Homing/Adhesion Receptor or HUTCH I or Heparan Sulfate Proteoglycan or Hermes Antigen or Hyaluronate Receptor or Phagocytic Glycoprotein 1 or CD44) - Drugs in Development, 2021 provides in depth analysis on Cells Expressing CD44 Antigen (CDw44 or Epican or Extracellular Matrix Receptor III or GP90 Lymphocyte Homing/Adhesion Receptor or HUTCH I or Heparan Sulfate Proteoglycan or Hermes Antigen or Hyaluronate Receptor or Phagocytic Glycoprotein 1 or CD44) targeted pipeline therapeutics. The report provides comprehensive information complete with Analysis by Indications, Stage of Development, Mechanism of Action (MoA), Route of Administration (RoA) and Molecule Type. The report also covers the descriptive pharmacological action of the therapeutics, its complete research and development history and latest news and press releases. Additionally, the report provides an overview of key players involved ...
ECM2 encodes extracellular matrix protein 2, so named because it shares extensive similarity with known extracellular matrix proteins. 2012 Aug 7;5(236):re4. Blood. 2020 Mar 5;17(1):20. doi: 10.1186/s12987-020-00181-9. Not only does the extracellular matrix hold the cells together to form a tissue, but it also allows the cells within the tissue to communicate with each other. 11 terms. 2003 Jun;9(6):685-93. doi: 10.1038/nm0603-685. Extensive extracellular matrix (ECM) remodeling is a hallmark of metastatic pancreatic ductal adenocarcinoma (mPDA). The extracellular matrix (ECM) of a blood vessel contributes substantially to the diverse functions of the blood vessel. And just as weve talked about the insides of a cell, not just being a bunch of organelles floating around that we have a cytoskeleton that gives the inside of the cell structure and allows it to even, potentially, move and divide and transport things. COVID-19 is an emerging, rapidly evolving situation. Del Monte-Nieto G, Fischer JW, ...
Sigma-Aldrich offers abstracts and full-text articles by [Hanne Haslene-Hox, Eystein Oveland, Kathrine Woie, Helga B Salvesen, Olav Tenstad, Helge Wiig].
In this paper we review what is known about the organization of adhesion plaques, the regions where cells in culture adhere most tightly to the underlying substratum. These specialized areas of the plasma membrane serve as attachment sites for stress fibres. A major objective has been to determine how microfilament bundles are anchored at such regions. In their morphology and composition adhesion plaques resemble the adhesions fibroblasts make to the extracellular matrix. Some extracellular matrix components have been identified on the outside face of adhesion plaques. Within the plasma membrane of adhesion plaques, extracellular matrix receptors, such as the fibronectin receptor (integrin), have been identified. This transmembrane glycoprotein complex has been shown to bind the cytoplasmic protein talin, which, in turn, associates with vinculin. These proteins establish a transmembrane chain of attachment between the extracellular matrix and the cytoskeleton, although how the actin filaments ...
a cytonaute of molecular size traveling toward a cell, before reaching the plasma membrane, would first need to go through a jungle of stems, branches, rain forest vines, and lianas. In tissues, this messy tangle is the extracellular matrix. Extracellular matrix is a scaffold of proteins and carbohydrates located around the cells that is synthesized by the cells themselves. Some authors duggest that this definition only applies to the insoluble components of the extracellular matrix. Extracellular matrix was invented by multicellular organisms. It was needed to keep cells together by adhesion, and therefore tissues appeared. During evolution, extracellular matrix got many other functions, not just adhesion, such as being responsible for the mechanical properties of most tissues (both in plant and animals), keeping cell morphology, allowing cell communication, setting pathways for cell migration, modulating cell differentiation and physiology, keeping growth factors in some places, and many ...
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Sigma-Aldrich offers abstracts and full-text articles by [Samantha D Smith, Ruhul H Choudhury, Patricia Matos, James A Horn, Stephen J Lye, Caroline E Dunk, John D Aplin, Rebecca L Jones, Lynda K Harris].
1. Dickstein K, Cohen-Solal A, Filippatos G, McMurray JJ, Ponikowski P, Poole-Wilson PA. et al. ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure 2008: the Task Force for the Diagnosis and Treatment of Acute and Chronic Heart Failure 2008 of the European Society of Cardiology. Developed in collaboration with the Heart Failure Association of the ESC (HFA) and endorsed by the European Society of Intensive Care Medicine (ESICM). Eur Heart J. 2008;29:2388-442 2. Graham HK, Horn M, Trafford AW. Extracellular matrix profiles in the progression to heart failure. European Young Physiologists Symposium Keynote Lecture-Bratislava 2007. Acta Physiol (Oxf). 2008;194:3-21 3. Yamazaki T, Lee JD, Shimizu H, Uzui H, Ueda T. Circulating matrix metalloproteinase-2 is elevated in patients with congestive heart failure. Eur J Heart Fail. 2004;6:41-5 4. George J, Patal S, Wexler D, Roth A, Sheps D, Keren G. Circulating matrix metalloproteinase-2 but not matrix metalloproteinase-3, ...
Trappmann, B and Gautrot, JE and Connelly, JT and Strange, DG and Li, Y and Oyen, ML and Cohen Stuart, MA and Boehm, H and Li, B and Vogel, V and Spatz, JP and Watt, FM and Huck, WT (2012) Extracellular-matrix tethering regulates stem-cell fate. Nat Mater, 11. 742-. ISSN 1476-1122. Full text not available from this repository ...
Regular exercise reduces cardiovascular and metabolic disease partly through improved aerobic fitness. The determinants of exercise-induced gains in aerobic fitness in humans are not known. We have demonstrated that over 500 genes are activated in response to endurance-exercise training, including modulation of muscle extracellular matrix (ECM) genes. Real-time quantitative PCR, which is essential for the characterization of lower abundance genes, was used to examine 15 ECM genes potentially relevant for endurance-exercise adaptation. Twenty-four sedentary male subjects undertook six weeks of high-intensity aerobic cycle training with muscle biopsies being obtained both before and 24 h after training. Subjects were ranked based on improvement in aerobic fitness, and two cohorts were formed (n = 8 per group): the high-responder group (HRG; peak rate of oxygen consumption increased by +0.71 ± 0.1 L min-1; p | 0.0001) while the low-responder group (LRG; peak rate of oxygen consumption did not change, +0
Significant progress has been achieved toward elucidating the molecular mechanisms that underlie breast cancer progression; yet, much less is known about the associated cellular biophysical traits. To this end, we use time-lapsed confocal microscopy to investigate the interplay among cell motility, three-dimensional (3D) matrix stiffness, matrix architecture, and transforming potential in a mammary epithelial cell (MEC) cancer progression series. We use a well characterized breast cancer progression model where human-derived MCF10A MECs overexpress either ErbB2, 14-3-3ζ, or both ErbB2 and 14-3-3ζ, with empty vector as a control. Cell motility assays showed that MECs overexpressing ErbB2 alone exhibited notably high migration speeds when cultured atop two-dimensional (2D) matrices, while overexpression of 14-3-3ζ alone most suppressed migration atop 2D matrices (as compared to non-transformed MECs). Our results also suggest that co-overexpression of the 14-3-3ζ and ErbB2 proteins facilitates ...
Mathematical Institute, Leiden University, The Netherlands. Title: Stigmergy in blood vessel growth: how indirect mechanical and chemical signaling, via the extra-cellular matrix, can coordinate collective cell behavior. Abstract: Angiogenesis, the formation of new blood vessels sprouting from existing vessel, occurs in several situations like wound healing, tissue remodeling, and near growing tumors. Under hypoxic conditions, tumor cells secrete growth factors, including VEGF. VEGF activates endothelial cells (ECs) in nearby vessels, leading to the migration of ECs out of the vessel and the formation of growing sprouts. A key process in angiogenesis is cellular self-organization, and previous modeling studies have identified mechanisms for producing networks and sprouts. Most theoretical studies of cellular self-organization during angiogenesis have ignored the interactions of ECs with the extra-cellular matrix (ECM), the jelly or hard materials that cells live in. Apart from providing ...
S. and Pederson, T. (1973) Biochem. 72,2766-2773. ; and Kim, J. (1974) Arch. Bioch. 767,1-10. E. (1964) P. NAS US 52,93-100. H. 251-276, (L. , Amsterdam. E. (1963) P. NAS US 50,1026-1032. ; and Harbers, E. (1973) Bioc. Biop. A. 557,295-304. A. (1972) P. NAS US 69,3417-3421. ; and Stambaugh, R. (1974) Arch. Bioch. 767,11-19. Y. and Bonner, J. (1970) J. Mol. Biol. 45,469-487. H. L. (1962) J. Mol. Biol. 5,172-184. J. (1974) Science 755,817-824. 34 EXTRACELLULAR MATRIX INFLUENCES ON GENE EXPRESSION 24. REFERENCES 1. Grobstein, C. (1954) Tissue interaction in the morphogenesis of mouse embryonic rudiments in vitro. In Aspects of Synthesis and Order in Growth, pp. 233-256, Princeton Univ. Press, Princeton, New Jersey. 2. Grobstein, C. (1948) Optimum gonopodial morphogenesis in Platypoecilus maculatus with constant dosage of methyl testosterone. J. Exp. Zool. 109:215-237. 3. Weiss, P. (1947) The problem of specificity in growth and development. Yale J. Biol. and Med. 19:235-278. 4. Grobstein, C. ...
In some cases, the ECM accounts for more of the organisms bulk than its cells. Extracellular matrix (ECM) All cells in solid tissue are surrounded by extracellular matrix. ______________ patterns are possible within elastin. _________________ and other ECM proteins are embedded in the matrix of aggrecans. This is especially important in the biomechanical tissues of _________, ___________ and __________. It is occupied by a kind of aqueous gel of polysaccharides and fibrous proteins, together with other molecules dispersed in it, such as electrolytes, enzymes and chemical transmitters. The focus of these questions includes topics such as the structure and function of the extracellular matrix (ECM). Explain how this feature contributes to non-compressibility. A deformation in the ECM can lead to __________________ of the integrin receptor. Proteins of the ECM provide structure and allow ________ _______________. Is there a little or large amount of elastin in tendons, skin, and loose connective ...
Here researchers employ three-dimensional culture systems for conditional gene targeted primary mouse embryonic fibroblasts that better simulate the reciprocal and adaptive interactions between cells and surrounding matrix, to define the role of Cdc42 signaling pathways in extracellular matrix organization. [J Biol Chem] Abstract ...
Unitary surgical devices (10) are disclosed. One group of the illustrated devices has a pair of biocompatible, bioresorbable anchors (16,18) connected to fixed lengths suture. The anchors (16,18) and fixed length of suture are connected to each other prior to surgery. Another group of unitary surgical devices has a pair of fixating mechanisms (15,17) connected to a base (21) prior to surgery. The second group of illustrated devices generally includes extracellular matrix material either as part of the base (21) or supported on the base (21). The extracellular matrix material serves as tissue regenerating material. In the second group of unitary surgical devices, the fixating mechanisms illustrated generally comprise suture, anchors or pre-formed holes in the base. All of the illustrated unitary surgical devices are useful in repairing a damaged meniscus. The first group of unitary surgical devices can be used to approximate inner surfaces of a tear in the meniscus. The second group of devices can be
Fingerprint Dive into the research topics of Dual-site recognition of different extracellular matrix components by anti-angiogenic/neurotrophic serpin, PEDF. Together they form a unique fingerprint. ...
This is not the fifth installment of the Matrix series, but in some senses the Extracellular Matrix is truly fundamental to our existence. The Extracellular Matrix is the vital fluid around our cells in each of our tissues. This matrix constantly changes, remodels, activates, degrades and repairs its components. In many tissues its responsible for laying down collagen and elastin. The matrix has to have balance between synthesis and degradation by matrix metalloproteinases (MMPs), which are further controlled by activators and tissue inhibitors of MMPs (which is why we all use our Alastin products, more of that in another post!). Remodeling of the matrix, both the activation and the destruction to make way for the new are regulated growth factors.. Growth factors have to work through integrins are transmembrane receptors that facilitate cell-extracellular matrix (ECM) adhesion, and regulate the cells to perform their functions. In the vagina the cells that have heathy are fibroblasts. Vaginal ...
Synonyms for Extracellular matrix in Free Thesaurus. Antonyms for Extracellular matrix. 1 antonym for extracellular: intracellular. What are synonyms for Extracellular matrix?
Thesis for Medicine Doctorate at Sahlgrenska Academy, Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry
Abstract The formation and remodeling of the embryonic valves is a complex and dynamic process that occurs within a constantly changing hemodynamic environment. Defects in embryonic and fetal valve remodeling are the leading cause of congenital heart defects, yet very little is known about how fibrous leaflet tissue is created from amorphous gelatinous masses called cushions. Microenvironmental cues such as mechanical forces and extracellular matrix composition play major roles in cell differentiation, but almost all research efforts in valvulogenesis center around genetics and molecular approaches. This review summarizes what is known about the dynamic mechanical and extracellular matrix microenvironment of the atrioventricular and semilunar valves during embryonic development and their possible guidance roles. A variety of new computational tools and sophisticated experimental techniques are progressing that enable precise microenvironmental alterations that are critical to complement genetic gain and
When DArcy Wentworth Thompsons On Growth and Form was published 100 years ago, it raised the question of how biological forms arise during development and across evolution. In light of the advances in molecular and cellular biology since then, a succinct modern view of the question states: how do genes encode geometry? Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales.. Read the Editorial by guest editors Thomas Lecuit and L. Mahadevan, as they provide a perspective on the influence of DArcy Thompsons work and an overview of the articles in this issue.. ...
Exhibits extracellular matrix binding activity. Involved in cell adhesion and extracellular matrix organization. Localizes to extracellular matrix and extracellular space. Is expressed in several structures, including central nervous system; genitourinary system; heart and pericardium; sensory organ; and skeleton. Used to study corneal dystrophy. Human ortholog(s) of this gene implicated in corneal dystrophy (multiple). Orthologous to human TGFBI (transforming growth factor beta induced ...
Elevations in myocardial stress initiate structural remodeling of the heart in an attempt to normalize the imposed stress. This remodeling consists of cardiomyocyte hypertrophy and changes in the amount of collagen, collagen phenotype and collagen cross-linking. Since fibrillar collagen is a relativ …
|h6>Highlights|/h6> |ul> |li>miRNA expression is altered in cancer, often through aberrant methylation |/li> |li>Altered expression of miRNAs that regulate extracellular matrix gene expression is associated with development of metastatic cancer |/li> |li>Novel approaches exploiting miRNA technology will aid in development of new treatments and diagnostic tools|/li> |/ul>
The present invention relates to an apparatus and a method for sealing a puncture in a tubular tissue structure or the wall of a body cavity. More specifically, the present invention is directed to an apparatus and method for sealing a puncture site in the wall of a tubular tissue structure, or in the wall of a body cavity with submucosal tissue or another extracellular or matrix-derived tissue capable of remodeling endogenous connective tissue in vivo. The submucosal tissue or another extracellular matrix-derived tissue is inserted into the puncture site as a sheet on an introducer element such as a needle, a cannula, a guide wire, an introducer element adapted for dialysis, an introducer element adapted for catheterization, a trocar, or any other introducer element used to access the lumen of a tubular tissue structure or used to access a body cavity.
Abstract Epithelial ovarian cancer is the sixth most common cancer in women worldwide and the most important cause of death from gynaecological cancers in the Western world. Our explorative pathway analysis on seven published gene-sets associated with platinum resistance in ovarian cancer reveals TP53 and transforming growth factor beta as key genes. Furthermore, the extracellular matrix was associated with chemotherapy resistance in ovarian cancer as well as endocrine resistance in breast cancer. Pathway analysis again revealed transforming growth factor beta as a key gene regulating extracellular matrix gene expression. A model is presented based on literature linking transforming growth factor beta, extracellular matrix, integrin signalling, epithelial to mesenchymal transition and regulating microRNAs with a (bivalent) role in chemotherapy response.. ...
Cell / extracellular matrix signaling[edit]. The extracellular matrix is composed of glycoproteins (proteins and ... In biology, juxtacrine signalling (or contact-dependent signalling) is a type of cell / cell or cell / extracellular matrix ... An extracellular matrix glycoprotein and a membrane protein interact.. Additionally, in unicellular organisms such as bacteria ... Cell adhesion, mechanical adhesion between cells and/or the extracellular matrix. *Role of cell adhesions in neural development ...
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Extracellular matrix. *Matrix gla protein. This article incorporates text from the public domain Pfam and InterPro IPR001393 ...
extracellular region. • elastic fiber. • extracellular matrix. • collagen-containing extracellular matrix. Biological process. ... extracellular matrix structural constituent. • protein binding. Cellular component. • proteinaceous extracellular matrix. • ... extracellular matrix disassembly. • extracellular matrix organization. • blood circulation. • respiratory gaseous exchange. ... into elastin fibres immediately after their synthesis by the cell and during their export into the extracellular matrix. ...
The roles of such proteins include protection and support, forming connective tissue, tendons, bone matrices, and muscle fiber ... Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
extracellular exosome. • basal lamina. • extracellular matrix. • collagen-containing extracellular matrix. Biological process. ... extracellular matrix structural constituent. • protein binding. Cellular component. • proteinaceous extracellular matrix. • ... extracellular matrix organization. • regulation of cell migration. • regulation of embryonic development. • negative regulation ... Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They ...
extracellular matrix Reticular fibers. Form a scaffolding for other cells. Type III collagen. liver, bone marrow, and lymphatic ... Both the ground substance and proteins (fibers) create the matrix for CT. Connective tissues are derived from the mesenchyme. ... Cells are spread through an extracellular fluid.. *Ground substance - A clear, colorless, and viscous fluid containing ...
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Extracellular Matrix Biochemistry. Elsevier, New York, 1984. Reddi A.H. (Ed.), Extracellular Matrix: Structure and Function. A. ... Homology of bone-inductive proteins from human, monkey, bovine, and rat extracellular matrix. Proc Natl Acad Sci U S A 1983; 80 ... The Reddi laboratory has also made important discoveries unraveling the role of the extracellular matrix in bone and cartilage ... They demonstrated first that BMPs bind the extracellular matrix, are present at the apical ectodermal ridge in the developing ...
extracellular matrix binding. Компонент клетки. • proteinaceous extracellular matrix. • cytoplasm. • integral component of ... Tomkowicz B., Rybinski K., Foley B., et al. Interaction of endosialin/TEM1 with extracellular matrix proteins mediates cell ... extracellular vesicular exosome. Биологический процесс. • biological_process. • positive regulation of cell proliferation. • ...
Extracellular matrix Bert JL; Pearce RH (1984). The interstitium and microvascular exchange. In: Handbook of Physiology. The ... The interstitial compartment is composed of connective and supporting tissues within the body - called the extracellular matrix ...
Kühn, Klaus (1997). "Extracellular matrix constituents as integrin ligands". In Elbe, Johannes A. (ed.). Integrin-ligand ... Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. They are a major component of the ... The trimeric proteins intersect to form a cross-like structure that can bind to other cell membrane and extracellular matrix ... M. A. Haralson; John R. Hassell (1995). Extracellular matrix: a practical approach. Ithaca, N.Y: IRL Press. ISBN 978-0-19- ...
... s are extracellular matrix glycoproteins. They are abundant in the extracellular matrix of developing vertebrate ... One mechanism to explain this may come from its ability to bind to the extracellular matrix glycoprotein fibronectin and block ...
... extracellular matrix glycoproteins. Vertebrate Emu proteins, which could interact with several different extracellular matrix ... Proteins known to contain an EMI domain include: Vertebrate Emilins, extracellular matrix glycoproteins. Vertebrate Multimerins ... The EMI domain is most often found at the N terminus of metazoan extracellular proteins that are forming or are compatible with ... a novel cysteine-rich domain of EMILINs and other extracellular proteins, interacts with the gC1q domains and participates in ...
Plasticity and the extracellular matrix. Archived 2007-12-03 at the Wayback Machine Hensch TK (2005). "Critical period ...
... extracellular Extracellular matrix; D. Furst, muscle; Joseph Bonventre, kidney and related subjects; P. Sutovsky, reproductive ...
Dietz, J (2007). "Arterial stiffness and extracellular matrix". Atherosclerosis, Large Arteries and Cardiovascular Risk. Adv. ...
Anatomy of corals Sarras, M. P.; Madden, M. E.; Zhang, X.; Gunwar, S.; Huff, J. K.; Hudson, B. G. (1991). "Extracellular matrix ... a more-or-less solid but loosely organized tissue consisting of a gel matrix [the mesoglea, in strict sense] with various ...
Extracellular Matrix Biomaterials for Soft Tissue Repair. Clin Podiatr Med Surg 26 (2009) 507-523 Article Valentin JE, Badylak ... gTissue is a type of living Tissue (biology), hence an ensemble of cells and extracellular matrix that carry out a particular ... Extracellular matrix bioscaffolds for orthopaedic applications. A comparative histologic study. J Bone Joint Surg Am 2006;88(12 ... For example, during tendon augmentation procedures, the collagen fiber architecture of the extracellular matrix can transition ...
"Signal transduction from the extracellular matrix". The Journal of Cell Biology. 120 (3): 577-85. doi:10.1083/jcb.120.3.577. ... Actin-adhesion links to the extracellular matrix". Nature Milestones. doi:10.1038/nrm2568. Burridge K, Feramisco JR; Feramisco ... "Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal ...
An extracellular matrix (ECM) is also present in metazoans. Its composition varies between cells, but collagens are the most ... Extracellular matrix Bacterial cell structure Plant cell Romaniuk JA, Cegelski L (October 2015). "Bacterial cell wall ... Frantz C, Stewart KM, Weaver VM (December 2010). "The extracellular matrix at a glance". Journal of Cell Science. 123 (Pt 24): ... Roberts K (October 1994). "The plant extracellular matrix: in a new expansive mood". Current Opinion in Cell Biology. 6 (5): ...
Accumulation of extracellular matrix proteins then occurs due to insufficient degradation by matrix metalloproteinases. ... Mesangial cells are separated by intercellular spaces containing extracellular matrix called the mesangial matrix that is ... Mesangial matrix provides structural support for the mesangium. Mesangial matrix is composed of glomerular matrix proteins such ... Mason, R; Wahab, N (2003). "Extracellular Matrix Metabolism in Diabetic Nephropathy". Journal of the American Society of ...
As the natural extracellular matrix (ECM) is important in the survival, proliferation, differentiation and migration of the ... Tibbitt, Mark W.; Anseth, Kristi S. (2009). "Hydrogels as extracellular matrix mimics for 3D cell culture". Biotechnology and ... Geckil, Hikmet; Xu, Feng; Zhang, Xiaohui; Moon, SangJun; Demirci, Utkan (2010). "Engineering hydrogels as extracellular matrix ... Hydrogel from wood-based nanofibrillated cellulose (NFC) is used as a matrix for 3D cell culture. As plant based material, it ...
Bou-Gharios G, Ponticos M, Rajkumar V, Abraham D (2004). "Extra-cellular matrix in vascular networks". Cell Prolif. 37 (3): 207 ...
... scaffolds are used in bone tissue engineering to mimic the natural extracellular matrix of the bones. The bone tissue ... Mo XM, Xu CY, Kotaki M, Ramakrishna S (May 2004). "Electrospun P(LLA-CL) nanofiber: a biomimetic extracellular matrix for ... In tissue engineering, a highly porous artificial extracellular matrix is needed to support and guide cell growth and tissue ... An electrospun nanofiber network resembles the extracellular matrix (ECM) well. This resemblance is a major advantage of ...
Papilin is an extracellular matrix glycoprotein. GRCh38: Ensembl release 89: ENSG00000100767 - Ensembl, May 2017 GRCm38: ...
Weber KT, Swamynathan SK, Guntaka RV, Sun Y (1999). "Angiotensin II and extracellular matrix homeostasis". The International ... Marked upregulation of genes encoding extracellular matrix components". Circulation Research. 75 (1): 23-32. doi:10.1161/01.res ... compensate with a number of structural alterations including hypertrophy of cardiomyocytes and increase of extracellular matrix ...
... mineralized tissues and extracellular matrix biology; biomaterials, nanobiotechnology and tissue engineering; and population ...
This work focused on the importance of the extracellular matrix and the ability of cultures in artificial 3D matrices to ... As the natural extracellular matrix (ECM) is important in the survival, proliferation, differentiation and migration of the ... Geckil H, Xu F, Zhang X, Moon S, Demirci U (April 2010). "Engineering hydrogels as extracellular matrix mimics". Nanomedicine. ... Prestwich GD (August 2007). "Simplifying the extracellular matrix for 3-D cell culture and tissue engineering: a pragmatic ...
... extra-cellular polymers, nectar, root exudates and leachates, dissolved organic matter, extra-cellular matrix, mucilage). The ...
In particular, abnormal interactions between epithelial cells and the extracellular matrix are associated with the over- ...
extracellular matrix structural constituent. •protein binding. •extracellular matrix constituent conferring elasticity. • ... proteinaceous extracellular matrix. •mitochondrion. Processo biológico. •blood vessel remodeling. •skeletal muscle tissue ... extracellular matrix organization. •regulation of actin filament polymerization. •stress fiber assembly. Sources: Amigo / ...
GO:0005578 extracellular matrix. • plasma membrane. • extracellular region. • extracellular space. • intracellular anatomical ... extracellular matrix structural constituent. • structural constituent of egg coat. Cellular component. • integral component of ... collagen-containing extracellular matrix. Biological process. • positive regulation of inflammatory response. • positive ... The zona pellucida (ZP) is a specialized extracellular matrix that surrounds the oocyte and early embryo. It is composed of ...
collagen-containing extracellular matrix. العمليات الحيوية. • bone mineralization. • biomineral tissue development. • negative ...
... are often resistant to commonly used antifungal agents because of difficulty in penetrating the extracellular polymeric matrix. ...
Borrelia burgdorferi and the extracellular matrix". Trends in Microbiology. 15 (8): 350-4. doi:10.1016/j.tim.2007.06.003. PMID ... and hiding in the extracellular matrix, which may interfere with the function of immune factors.[89][90] ...
extracellular matrix Reticular fibers. Form a scaffolding for other cells. Type III collagen. liver, bone marrow, and lymphatic ... Both the ground substance and proteins (fibers) create the matrix for CT. Connective tissues are derived from the mesenchyme. ... Cells are spread through an extracellular fluid.. *Ground substance - A clear, colorless, and viscous fluid containing ...
They are a type of extracellular matrix (ECM) protein, which, together with ameloblastins, enamelins, and tuftelins direct the ... mineralization of enamel to form a highly organized matrix of rods, interrod crystal, and protein. Although the precise role of ...
MPP+ eventually travels to the extracellular fluid by a dopamine transporter, which ultimately causes the Parkinson's symptoms ...
... s can be derived from the endoplasmic reticulum and replicate by fission.[13] Peroxisome matrix proteins are ... of peroxisomal matrix proteins signals them to be imported into the organelle. There are at least 32 known peroxisomal proteins ... is translocated into the peroxisomal matrix and recycled to the cytosol". Cell. 105 (2): 187-96. doi:10.1016/s0092-8674(01) ... and may occur without the import of the matrix (lumen) enzymes. Proliferation of the organelle is regulated by Pex11p. ...
One of the parts of the extracellular matrix is a complex molecule called a proteoglycan. Like many components of the body, ... The matrix surrounds the cells of the body in an organized meshwork and functions as the glue that holds the cells of the body ... This matrix is made up of a variety of sugars and proteins and helps to form the architectural framework of the body. ... of Hunter syndrome is related to a problem in a part of the connective tissue of the body known as the extracellular matrix. ...
"PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix-dependent phosphatidylinositol 3- ... extracellular region. • extracellular. • synaptic vesicle. • axon. • dendrite. Biological process. • brain-derived neurotrophic ...
Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis For Repair and Tissue Engineering, M. Goldberg and A.J ... Dentin is a bone-like matrix that is porous and yellow-hued material. It is made up of 70% inorganic materials (mainly ... The innermost layer of dentin is known as predentin, and is the initial dentin matrix that is laid down prior to mineralisation ... Matrix metalloproteinases (MMPs) and their specific tissue inhibitors (TIMPs) in mature human odontoblasts and pulp tissue. ...
... sequences so the transplantation cells have closely related properties to that of native tissue in the extracellular matrix.[3] ... the inability of a sufficient matrix to thrive and create a uniform population of cells, or the migratory response of the cells ... by the body and biological functions such as cell adhesion and growth will be enhanced through cell-cell and cell-matrix ...
Beginning with a small 2 x 2 matrix, participants copy the matrix pattern from memory into an empty matrix. The matrix patterns ... Hebert, A. E.; Dash, P. K. (2002). "Extracellular signal-regulated kinase activity in the entorhinal cortex is necessary for ... The matrix patterns are arranged in a way that is difficult to code verbally, forcing the participant to rely on visual spatial ... At the end, the participant is asked to indicate on a real matrix where the little man that he or she visualized finished. The ...
extracellular matrix. • cytosol. • cell nucleus. • neuron projection. • neuronal cell body. Biological process. • muscle organ ... extracellular exosome. • stress fiber. • plasma membrane. • Z disc. • cytoplasm. • cell cortex. • focal adhesion. • ...
proteinaceous extracellular matrix. • extracellular region. • extracellular exosome. • extracellular space. • extracellular ... extracellular matrix constituent conferring elasticity. • identical protein binding. • integrin binding involved in cell-matrix ... It is the best characterized member of the EMILIN family of extracellular matrix glycoproteins. ... matrix. • integrin alpha4-beta1 complex. • EMILIN complex. Biological process. • cell adhesion. • cell-matrix adhesion. • cell ...
Extracellular matrix *Cell wall. Retrieved from "https://en.wikipedia.org/w/index.php?title=Autophagy&oldid=821997179" ...
Contrast extracellular.. introduced species. Also called an exotic species, foreign species, alien species, non-native species ... In eukaryotes, the cytoskeletal matrix is a dynamic structure composed of three main proteins, which are capable of rapid ... extracellular. Of or occurring in the space outside the plasma membrane of a cell. Contrast intracellular.. extranuclear ... The minus sign indicates that the inside of the cell is negative with respect to the surrounding extracellular fluid.. ...
extracellular exosome. • نواة. • منطقة خارج الخلية. • collagen-containing extracellular matrix. العمليات الحيوية. • ...
... accelerating extracellular matrix and collagen formation and thus reducing the time for the healing process to occur.[22] ... "The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor". Biomaterials. 35 ...
The majority of mammalian ova are covered in a layer of granulosa cells intertwined in an extracellular matrix that contains a ... By catalyzing the hydrolysis of hyaluronan, a constituent of the extracellular matrix (ECM), hyaluronidase lowers the viscosity ... "Matrix Biology. 20 (8): 499-508. doi:10.1016/S0945-053X(01)00172-X. PMID 11731267.. ... although exogenous hyaluronidases can disrupt the cumulus matrix. ...
extracellular matrix organization. • positive regulation of sequence-specific DNA binding transcription factor activity. • ... extracellular region. • plasma membrane. • membrane raft. • extracellular space. Biological process. • regulation of protein ...
Extracellular matrix. Retrieved from "https://en.wikipedia.org/w/index.php?title=Autophagy&oldid=869052817" ...
Ingelesez) «Extracellular matrix structure» Advanced Drug Delivery Reviews (97): 4-27 2016-02-01 doi:10.1016/j.addr.2015.11.001 ... Ingelesez) Mecham, Robert (2011-02-16) The Extracellular Matrix: an Overview Springer Science & Business Media ISBN ... Yue, Beatrice (2014) «Biology of the Extracellular Matrix: An Overview» Journal of glaucoma: S20-S23 doi:10.1097/IJG. ... The Extracellular Matrix of Animals» Molecular Biology of the Cell. 4th edition . Noiz kontsultatua: 2018-11-22 . ...
Additionally, the extracellular matrix and dense outer layer of bacterial cells can protect the inner bacteria cells from ... biofilms by adhering to surfaces on implanted devices such as catheters and prostheses and creating an extracellular matrix for ...
... matrix 1 protein), M2, NS1 (non-structural protein 1), NS2 (other name is NEP, nuclear export protein), PA, PB1 (polymerase ... "A universal influenza A vaccine based on the extracellular domain of the M2 protein". Nat. Med. 5 (10): 1157-63. doi:10.1038/ ...
extracellular region. • platelet alpha granule lumen. • extracellular. Biological process. • regulation of gene expression by ... and Matrix Attachment Region −3 (MAR3). These three DNA sequences bind to CTCF in a way that limits downstream enhancer access ...
Extracellular matrix. Illustration depicting extracellular matrix (basement membrane and interstitial matrix) in relation to ... The animal extracellular matrix includes the interstitial matrix and the basement membrane.[5] Interstitial matrix is present ... Extracellular matrix: review of its roles in acute and chronic wounds. *Usage of Extracellular Matrix from pigs to regrow human ... In biology, the extracellular matrix (ECM) is a three-dimensional network of extracellular macromolecules, such as collagen, ...
Media in category "Extracellular matrix proteins". The following 85 files are in this category, out of 85 total. ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s007.ogv 15 s, ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s008.ogv 15 s, ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s009.ogv 15 s, ...
Matrigel Matrix , Extracellular Matrix , Corning. We use cookies to ensure the best experience on our website. ... Cells behave better on Corning Matrigel matrix-the original, trusted extracellular matrix (ECM).. Nearly 30 years ago, ... Corning Matrigel Matrix FAQs Corning Matrigel Matrix FAQs Get answers to commonly asked questions about Corning Matrigel matrix ... Extracellular matrices are complex biological reagents, and, like all biologically derived reagents, they may be subject to lot ...
Used to study apoptosis, cell signaling pathways and gene expression. MP Biomedicals Hormones: Dexamethasone acts as anti-inflammatory glucocorticoid that induces the production of phospholipase A2 inhibitory protein (lipocortin). ...
... by the extracellular matrix. The proteins and glycoconjugates that make up the extracellular matrix provide structural support ... Biology of Extracellular Matrix. Copyright. 2013. Publisher. Springer-Verlag Berlin Heidelberg. Copyright Holder. Springer- ... The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement ... Biology of Extracellular Matrix Free Preview © 2013. Evolution of Extracellular Matrix. Editors: Keeley, Fred W., Mecham, ...
Stress relaxation properties of the matrix as well as water transport through aquaporin-1 enable extracellular vesicles to ... which requires EVs to traverse the extracellular matrix (ECM). However, given that the size of EVs is usually larger than the ... Matrix stress relaxation allows EVs to overcome the confinement, and a higher crosslinking density facilitates a fluctuating ... Using engineered hydrogels, we demonstrate that the mechanical properties of the matrix regulate anomalous EV transport under ...
The extracellular matrix (ECM) is an acellular three-dimensional network composed of proteins, glycoproteins, proteoglycans and ... "Extracellular Composite Matrices in Arthropods" published by Springer in 2016.. Hans Merzendorfer is appointed as a Professor ...
Many of the interactions have also been demonstrated on tissue sections or in vivo, and adherence to the extracellular matrix ... This review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the ... Some of these bacterial proteins are highly specific for an extracellular matrix protein, some are multifunctional and express ... Pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e ...
The extracellular matrix of the human optic nerve.. Goldbaum MH1, Jeng SY, Logemann R, Weinreb RN. ... The nerve fibers of the optic nerve are enclosed and segmented by extracellular matrix. With immunostains, we localized ... and fibronectin in frozen sections of the extracellular matrix of the prelaminar, laminar, and retrolaminar human optic nerve. ... The internal limiting lamina of the optic nerve has an extracellular composition similar to the thicker adjacent retinal ...
... Michael G. Hahn hahn at MOND1.CCRC.UGA.EDU Tue Nov 14 15:26:40 EST 1995 * ... Matrix Polysaccharides, Callose, and Cellulose Deborah Delmer, Bruce Wasserman, John Ralph Role of the Extracellular Matrix in ... roles of the extracellular matrix in plant development and in interaction= s with sybiotic and pathogenic organisms, and the ... The Extracellular Matrix of Plants: Molecular, Cellular and Developmental Biology Tamarron, Colorado. March 15-21, 1996 The ...
Here we demonstrate the ability of decellularized liver extracellular matrix (dECM) hydrogels to induce the in vitro formation ... Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels. Tissue Eng. Part A 16, 1075-1082 ... Complex bile duct network formation within liver decellularized extracellular matrix hydrogels. *Phillip L. Lewis1,2. , ... The extracellular matrix (ECM) of the developing liver plays an integral role in the formation and maturation of the biliary ...
Kliniken & Institute … Kliniken Zentrum für Innere… Innere Medizin III:… Forschung Grundlagenforschung … Extracellular Matrix… ... AG Extracellular Matrix and Integrins in Cardiovascular Disease. Integrin signaling and heart failure Integrins are ... By establishing a Tamoxifen (Tx) inducible knockout mouse, we have demonstrated that the extracellular matrix glycoprotein ... In this project we analyze effects of extracellular cues for hypertrophic remodeling and examine transcription factor circuits ...
... Nat Mater. 2012 May 27;11(7):642-9. doi: 10.1038/nmat3339. ... could not form stable focal adhesions and differentiated as a result of decreased activation of the extracellular-signal- ...
... is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and blood vessels. ... a new extracellular matrix glycoprotein localized on collagen fibrils. Matrix Biol 15:11-19PubMedCrossRefGoogle Scholar ... Tenascin-X (TNX) is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and ... Bristow J, Tee MK, Gitelman SE, Mellon SH, Miller WL (1993) Tenascin-X: a novel extracellular matrix protein encoded by the ...
1988) Extracellular matrix specificity for the differentiation of capillary endothelial cells. Exp. Cell Res., 178: 426-434. ... 1991) Effects of soluble factors and extracellular matrix components on vascular cell behavior in vitro and in vivo: models of ... Madri, J.A., Pratt, B.M., and Yannariello-Brown, J.(1989) Endothelial cell-extracellular matrix interactions. In: Endothelial ... 1978) An ultrastructural study of developing extracellular matrix in vitelline blood vessels of the early chick embryo. Am. J. ...
In biology, the extracellular matrix (ECM) is the extracellular part of multicellular structure (e.g., organisms, tissues, and ... to pro-anabolic upon integration to the extracellular matrix," Matrix Biology, vol. 31, no. 5, pp. 290-298, 2012. View at ... P. G, "The extracellular matrix and cell adhesion," in Cells, B. Lewin, L. Cassimeris, V. Lingappa, and G. Plopper, Eds., 2007. ... "Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia," Matrix ...
Extracellular matrix composition significantly influences pancreatic stellate cell gene expression pattern: role of transgelin ...
1993) Extracellular matrix 3: Evolution of the extracellular matrix in invertebrates. FASEB J 7:1115-1123.. ... Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro. Yael Helman, Frank Natale, Robert ... 2000) Extracellular matrix (ECM) components in a very primitive multicellular animal, the dicyemid mesozoan Kantharella ... Extracellular production of organic matrices and calcium carbonate particles was examined in primary, nondividing cell cultures ...
Bonnans C, Chou J, Werb Z. Remodelling the extracellular matrix in development and disease. Nat Rev Mol Cell Biol. 2014;15(12): ... Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques. Sarah R. Langley,1,2 Karin ... The subendothelial extracellular matrix modulates JNK activation by flow. Circ Res. 2009;104(8):995-1003.. View this article ... Extracellular matrix composition and remodeling in human abdominal aortic aneurysms: a proteomics approach. Mol Cell Proteomics ...
There is described herein methods of treating a disease associated with extracellular matrix (ECM) in a patient. In some cases ... 1 shows extracellular matrix accumulation (ECM) in normal and fibrotic skin corresponds to a shift in metabolism from fatty ... 21 shows extracellular matrix deposition in the murine skin fibrosis after CD36high fibroblast vs. vehicle treatment. Tissue ... The skin is the largest organ of the human body and 70% of its dry weight is comprised of extracellular matrix (ECM). The ...
Immobilization after injury alters extracellular matrix and stem cell fate. Amanda K. Huber,1 Nicole Patel,1 Chase A. Pagani,1 ... Cells sense extracellular environment and mechanical stimuli and translate these signals into intracellular responses through ...
A protective extracellular matrix underpins HIV infectivity. All press releases are available in the Press area of the Institut ... A protective extracellular matrix underpins HIV infectivity. A "viral biofilm" (in green) produced at the surface of a CD4 T ... HomePress areaPress documentsA protective extracellular matrix underpins HIV infectivity ... transported in an adhesive extracellular matrix, which also shelters them from the immune system and antiretroviral drugs. "We ...
... Curr Opin Cell Biol. 1996 Oct;8(5):625-31. doi: ...
The impact of decellularization agents on renal tissue extracellular matrix Nafiseh Poornejad, Lara B. Schaumann, Travis ... leaving intact collagenous extracellular matrices (ECMs). Although complete removal of cellular remnants should be achieved to ...
The purpose of this review is to summarize the established and proposed changes to the hepatic extracellular matrix (ECM) that ... The fibrotic stage of ALD is primarily characterized by robust accumulation of extracellular matrix (ECM) proteins (collagens) ... "The extracellular matrix: tools and insights for the omics era," Matrix Biology, vol. 49, pp. 10-24, 2016. View at: Publisher ... The extracellular matrix (ECM) consists of a diverse range of components that work bidirectionally with surrounding cells to ...
... extracellular matrix organization, positive regulation of cell-substrate adhesion ... Extracellular region or secreted. *extracellular matrix Source: GO_CentralInferred from biological aspect of ancestori*. " ... extracellular matrix organization Source: GO_CentralInferred from biological aspect of ancestori*. "Phylogenetic-based ... Extracellular matrix protein 2Imported. ,p>Information which has been imported from another database using automatic procedures ...
... and postsynaptic neuronal elements with astroglial processes and extracellular matrix (ECM) for formation and plasticity of ... Here we outline the major mechanisms driving: (i) synaptogenesis by secreted extracellular scaffolding molecules, like ... Here we outline the major mechanisms driving (i) synaptogenesis by secreted extracellular scaffolding molecules, like ... Particularly, we focused on potential importance of activity-dependent concerted activation of multiple extracellular proteases ...
Hironobu Fujiwara on Cutaneous extracellular matrix, part of a collection of online lectures. ... Cutaneous extracellular matrix. *Dr. Hironobu Fujiwara - RIKEN Center for Developmental Biology (CDB) Kobe, Japan ... Fujiwara, H. (2014, May 4). Cutaneous extracellular matrix [Video file]. In The Biomedical & Life Sciences Collection, Henry ... Today Id like to talk about several of the extracellular matrices, ECM in mammalian skin. ...
Review articles on Matrix extracellular phosphoglycoprotein Articles on Matrix extracellular phosphoglycoprotein in N Eng J Med ... Ongoing Trials on Matrix extracellular phosphoglycoprotein at Clinical Trials.gov Trial results on Matrix extracellular ... Patient Handouts on Matrix extracellular phosphoglycoprotein Directions to Hospitals Treating Matrix extracellular ... Most recent articles on Matrix extracellular phosphoglycoprotein Most cited articles on Matrix extracellular ...
  • Glycosaminoglycans (GAGs) are carbohydrate polymers and mostly attached to extracellular matrix proteins to form proteoglycans (hyaluronic acid is a notable exception, see below). (wikipedia.org)
  • In the extracellular matrix, especially basement membranes , the multi-domain proteins perlecan , agrin , and collagen XVIII are the main proteins to which heparan sulfate is attached. (wikipedia.org)
  • The development of Corning Matrigel matrix, a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors. (corning.com)
  • The proteins and glycoconjugates that make up the extracellular matrix provide structural support to cellular complexes, facilitate cell adhesion and migration, and impart mechanical properties that are important for tissue function. (springer.com)
  • The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement membranes. (springer.com)
  • The extracellular matrix (ECM) is an acellular three-dimensional network composed of proteins, glycoproteins, proteoglycans and exopolysaccharides. (springer.com)
  • Bacterial proteins binding to the mammalian extracellular matrix. (nih.gov)
  • Pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e. collagens, laminin, fibronectin or proteoglycans. (nih.gov)
  • Some of these bacterial proteins are highly specific for an extracellular matrix protein, some are multifunctional and express binding activities towards a number of target proteins. (nih.gov)
  • The scheduled sessions include those on structural studies of the major macromolecules that make up the plant extracellular matrix (polysaccharides, proteins, lignin), biosynthesis of wall macromolecules,= roles of the extracellular matrix in plant development and in interaction= s with sybiotic and pathogenic organisms, and the importance of plant cell walls= in food and industry. (bio.net)
  • Cartilage extracellular matrix (ECM) is composed primarily of the network type II collagen (COLII) and an interlocking mesh of fibrous proteins and proteoglycans (PGs), hyaluronic acid (HA), and chondroitin sulfate (CS). (hindawi.com)
  • This protective mesh is composed principally of proteins and carbohydrates and is comparable to that of bacterial biofilm, an extracellular matrix network secreted by some bacteria as protection against their environment. (pasteur.fr)
  • 8,200 antibodies specific for proteins of the extracellular matrix. (antibodies-online.com)
  • Background: The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. (cellsignal.com)
  • Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). (cellsignal.com)
  • The glue is fibronectin, a cell adhesion protein that allows cells to attach to the different matrix elements, including collagen, GAGs, and integrins, membrane proteins that connect cells to the environment and play an important role in signaling cascades. (jove.com)
  • ECM2 encodes extracellular matrix protein 2, so named because it shares extensive similarity with known extracellular matrix proteins. (wikipedia.org)
  • AlphaD1 exits only of Alpha-helices, whereas SASDL2 and -3 are capable of binding most of the extracellular matrix proteins identified so far (collagen type IV, laminin 332, fibronectin , perlecan, fibulin 1C/D , fibulin-3 and MMP-9 ) (Sercu et al. (atlasgeneticsoncology.org)
  • Macromolecular crowding in extracellular culture media directly induced supramolecular assembly and alignment of extracellular matrix proteins deposited by cells, which in turn increased alignment of the intracellular actin cytoskeleton. (mit.edu)
  • This new edition of Guidebook to the Extracellular Matrix and Adhesion Proteins brings up-to-date information on the purification, activities, antibodies and genes for the proteins found in the extracellular matrix and those known to be involved in cell-cell contact and adhesion. (google.it)
  • Drawing on a wide range of sources, this volume addresses all aspects of the structure and function of modules in the extracellular matrix, adhesion molecules, cell adhesion and cell-cellcontact proteins. (google.it)
  • CD36 is a busy molecule, regularly making contact with a wide variety of proteins in the extracellular matrix and on neighboring cells. (thefreedictionary.com)
  • The carboxy terminus of dystrophin binds to a plasma membrane anchor, [beta]-dystroglycan, which is associated on the external side with the extracellular matrix receptor, [alpha]-dystroglycan, which binds to the basal lamina proteins laminin 1, laminin 2, and agrin. (thefreedictionary.com)
  • Thus interaction between integrins and specific matrix proteins is responsible for sensing mechanical strain in vascular smooth muscle cells. (jci.org)
  • to investigate the lymphocyte pathways regulating the viral biofilms formation and composition in extracellular matrix (ECM) proteins (using quantitative polymerase chain reaction (qPCR) and siRNA). (clinicaltrials.gov)
  • In this study we explored the effect of selective extracellular matrix (ECM) proteins on the potential of insulin-producing cell differen-tiation using ARIP cells, an adult rat pancreatic ductal epithelial cell line, as a model in vitro. (scirp.org)
  • Is the Subject Area "Extracellular matrix proteins" applicable to this article? (plos.org)
  • For example, matrix proteins sequester water that provides turgor to soft tissues and minerals that give rigidity to skeletal tissues. (humpath.com)
  • neurofascin-186 [NF-186] and neuron glia-related CAM [NrCAM]), cytoskeletal proteins (ankyrinG and βIV spectrin), and the extracellular chondroitin-sulfate proteoglycan brevican. (rupress.org)
  • Thus, the clustering of ion channels at nodes of Ranvier is thought to depend on binding to cytoskeletal and scaffolding proteins that are positioned along axons by extracellular, heterophilic interactions between axonal and glial CAMs ( Schafer and Rasband, 2006 ). (rupress.org)
  • Rather than linking two cells, hemi-desmosomes attach cells to the extracellular matrix and use integrin cell adhesion proteins rather than cadherins. (sabiosciences.com)
  • We have shown that, in vivo , SCLC cells are surrounded by an extensive stroma of extracellular matrix (ECM) at both primary and metastatic sites which contains, among other proteins, fibronectin, laminin and collagen IV. (clinsci.org)
  • Next, cell migration uses adhesion proteins on both the cell surface and in the extracellular matrix (ECM) to guide remodeling and cell movement through the environment. (jimmunol.org)
  • Tissue inhibitors of metalloproteinases (TIMPs) regulate the ECM turnover through negative regulation of matrix metalloproteinases (MMPs), which degrade the ECM structural proteins. (ahajournals.org)
  • 3 - 7 Matrix metalloproteinases (MMPs) degrade ECM proteins, whereas their proteolytic activity is kept in check by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). (ahajournals.org)
  • Proteolytic assays were performed with extracellular matrix proteins to assess the putative role of Cwp84 in the pathogenicity of C. difficile . (asm.org)
  • P47 shows strong and specific binding to gastrointestinal tissues and some extracellular matrix proteins (type I collagen, thrombospondin, and vitronectin) ( 4 ). (asm.org)
  • In fact, collagen is the most abundant protein in the human body [15] [16] and accounts for 90% of bone matrix protein content. (wikipedia.org)
  • Higher protein concentration provides greater matrix stiffness and scaffold integrity. (corning.com)
  • The other protein components of the BM are thought to be non-covalently immobilized in the matrix formed by laminin and collagen IV6. (sigmaaldrich.com)
  • However, cells on polyacrylamide of low elastic modulus (0.5 kPa) could not form stable focal adhesions and differentiated as a result of decreased activation of the extracellular-signal-related kinase (ERK)/mitogen-activated protein kinase (MAPK) signalling pathway. (nih.gov)
  • Tenascin-X (TNX) is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and blood vessels. (springer.com)
  • Bristow J, Tee MK, Gitelman SE, Mellon SH, Miller WL (1993) Tenascin-X: a novel extracellular matrix protein encoded by the human XB gene overlapping P450c21B. (springer.com)
  • In this regard, especially the matrix protein Tenascin C (Tnc) proved to be an important regulator of astrocyte precursor cell proliferation and migration during spinal cord development. (frontiersin.org)
  • Collagen I is a heterotrimeric extracellular matrix protein found in the skin and other connective tissues. (thermofisher.com)
  • Extracellular matrix protein 2 is a protein that in humans is encoded by the ECM2 gene. (wikipedia.org)
  • We quantified the impact of induced crowding on the extracellular and intracellular protein organization of human mesenchymal stem cells (MSCs) via immunocytochemistry, atomic force microscopy (AFM), and AFM-enabled nanoindentation. (mit.edu)
  • Cysteine rich 61 (CCN1) is an extracellular matrix (ECM) protein elevated in cancer cells that modulates their adhesion and migration by binding cell surface receptors. (aacrjournals.org)
  • It is a secreted protein which typically localizes in the extracellular matrix (ECM) and on the cell surface ( 5 ), in which it binds integrin receptors to modulate a variety of cellular functions including adhesion, migration, and proliferation ( 6 ). (aacrjournals.org)
  • Cells adhered, spread, and proliferated on each matrix protein, but the mitogenic response to strain was matrix dependent. (jci.org)
  • Here, the use of the C-C motif chemokine ligand 25 (CCL)25 in comparison to differentiation factors such as transforming growth factor (TGF)β3, bone morphogenetic protein (BMP)2, BMP7, BMP12, and BMP14 (all in concentrations of 10, 50 and 100 ng/mL) was tested in an in vitro micro mass pellet model with isolated and cultivated human AF-cells ( n = 3) to induce and enhance AF-matrix formation. (mdpi.com)
  • This work demonstrates a role for a brain-specific extracellular matrix protein in glioma invasion, opening new therapeutic avenues for a uniformly fatal disease. (jneurosci.org)
  • We now show that JNK activation by both onset of laminar flow and long-term oscillatory flow is matrix-specific, with enhanced activation on fibronectin compared to basement membrane protein or collagen. (ahajournals.org)
  • The composition of the matrix of the interstitium and the proportion and the expression of each protein can have a profound influence on cardiac structure and compliance that will determine its hemodynamic functions ( 20 ). (physiology.org)
  • In biology , the extracellular matrix ( ECM ) is a three-dimensional network of extracellular macromolecules , such as collagen , enzymes , and glycoproteins , that provide structural and biochemical support of surrounding cells. (wikipedia.org)
  • In biology, the extracellular matrix (ECM) is a three-dimensional network consisting of extracellular macromolecules and minerals, such as collagen, enzymes, glycoproteins and hydroxyapatite that provide structural and biochemical support to surrounding cells. (wikipedia.org)
  • The Extracellular matrix (ECM) is the network of extracellular macromolecules that provide cells structural and biochemical support. (peprotech.com)
  • These macromolecules are present in intercellular junctions and cell surfaces and may assemble into two general organizations: interstitial matrix and basement membrane (BM). (humpath.com)
  • The cells in tissue are in contact with secreted extracellular macromolecules referred to as the extracellular matrix . (humpath.com)
  • Thus, extracellular macromolecules influence and organize not only the basal cell surface but also the actin-rich basal cell cortex of epithelial cells. (rupress.org)
  • the myocardial extracellular matrix (ECM) represents a complex three-dimensional network of various macromolecules. (physiology.org)
  • This volume in the Biology of Extracellular Matrix series provides a review of the known classes of proteases that degrade ECM both outside and inside the cell. (springer.com)
  • The topics covered in this volume provide an important context for understanding the role that matrix-degrading proteases play in normal tissue remodeling and in diseases such as cancer and lung disease. (springer.com)
  • Comparative genomics has identified a conserved core ECM adhesome, that includes integrin adhesion receptors, matrix proteases and the central ECM components of connective ECM and basement membranes. (els.net)
  • Collagen is exocytosed in precursor form (procollagen), which is then cleaved by procollagen proteases to allow extracellular assembly. (wikipedia.org)
  • Particularly, we focused on potential importance of activity-dependent concerted activation of multiple extracellular proteases, such as ADAMTS4/5/15, MMP9 and neurotrypsin, for permissive and instructive events in synaptic remodeling through localized degradation of perisynaptic ECM and generation of proteolytic fragments as inducers of synaptic plasticity. (frontiersin.org)
  • Among proteases, the matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family are often associated with ECM degradation and remodeling. (sabiosciences.com)
  • The abstract deadline for the Keystone Meeting on The Extracellular Matri= x of Plants: Molecular, Cellular and Developmental Biology HAS BEEN EXTENDED to November 20, 1995. (bio.net)
  • [5] Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). (wikipedia.org)
  • [7] Some single-celled organisms adopt multicellular biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS). (wikipedia.org)
  • Cells behave better on Corning Matrigel matrix-the original, trusted extracellular matrix (ECM). (corning.com)
  • The evolution of single cells into multicellular organisms was mediated, in large part, by the extracellular matrix. (springer.com)
  • Cells release extracellular vesicles (EVs) to communicate over long distances, which requires EVs to traverse the extracellular matrix (ECM). (nature.com)
  • The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. (sigmaaldrich.com)
  • Here we demonstrate the ability of decellularized liver extracellular matrix (dECM) hydrogels to induce the in vitro formation of complex biliary networks using encapsulated immortalized mouse small biliary epithelial cells (cholangiocytes). (nature.com)
  • Cells attach to proteoglycans and glycoproteins on the surface of other cells as well as in the extracellular matrix (ECM) substratum via adhesion molecules to define tissue shape, structure, and function. (qiagen.com)
  • In biology, the extracellular matrix (ECM) is the extracellular part of multicellular structure (e.g., organisms, tissues, and biofilms) that typically provides structural and biochemical support to the surrounding cells [ 1 ]. (hindawi.com)
  • Integrins are transmembrane receptors that mediate the attachment between a cell and its surroundings, such as other cells or the extracellular matrix (ECM). (hindawi.com)
  • The evolution of multicellularity in animals required the production of extracellular matrices that serve to spatially organize cells according to function. (pnas.org)
  • In this report, we examine the production of these three matrices by using an in vitro culturing system for coral cells. (pnas.org)
  • Using HIV-infected CD4+ T cells, Dr Thoulouze and colleagues analyzed the extracellular mesh formed at the surface of these cells and evaluated viral infection after its destruction. (pasteur.fr)
  • Effective decellularization uses agents that lyse cells and remove all cellular materials, leaving intact collagenous extracellular matrices (ECMs). (aiche.org)
  • The agreement is based upon Kensey Nash 's proprietary tissue processing technology, known as the Optrix process, which gently disinfects tissues, inactivates viruses and removes cells while preserving extracellular matrix components. (medindia.net)
  • Human prolactin secreting adenoma cells maintained on extracellular matrix. (biomedsearch.com)
  • In vitro maintenance of human prolactin secreting adenoma cells on a extracellular matrix (ECM) produced by bovine corneal endothelial cells is associated with the adoption of morphological properties that are not expressed when the cells are seeded on plastic. (biomedsearch.com)
  • Both tumor cells and normal cells are more likely to resemble their in vivo counterparts when maintained on extracellular matrix than on plastic, and therefore, cells cultured on ECM may provide a better system for oncogenic and endocrine studies. (biomedsearch.com)
  • In biology, the extracellular matrix ( ECM ) is the extracellular part of animal tissue that usually provides structural support to the animal cells in addition to performing various other important functions. (medicalxpress.com)
  • Methods for culturing eukaryotic cells and studying their growth characteristics, including the invasive growth characteristics of tumor cells, on the submucosal matrix are described. (google.com)
  • Supporting many cellular tissues is the extracellular matrix, or ECM, an interconnected network composed of fibers and ground substance, which is mostly interstitial fluid that fills the space between the cells, connective tissue fibers, and capillaries. (jove.com)
  • In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). (jove.com)
  • Geltrex™ matrix is a soluble form of reduced growth factor (RGF) basement membrane extract (BME) purified from continuous sheets of specialized extracellular matrix that form an interface between Engelbreth-Holm-Swarm (EHS) tumor cells. (thermofisher.com)
  • Coloured scanning electron micrograph (SEM) of the extracellular matrix (ECM) found around multicellular aggregates of yeast cells, such as biofilms. (sciencephoto.com)
  • Are factors originating from serum, plasma, or cultured cells involved in the growth-promoting effect of the extracellular matrix produced by cultured bovine corneal endothelial cells? (thefreedictionary.com)
  • The molecules of the extracellular matrix (ECM) comprise the connective tissues of animals and form basal laminae underlying sheets of epithelial cells. (thefreedictionary.com)
  • Our work elucidated a highly intriguing question: how cells move when they are in the complex and physiologically relevant environment of a 3-D extracellular matrix ," said Hyun (Michel) Koo, a professor in the Department of Orthodontics at Penn's School of Dental Medicine. (thefreedictionary.com)
  • Mechanical strain of rat vascular smooth muscle cells is sensed by specific extracellular matrix/integrin interactions. (jci.org)
  • 7. The biomaterial scaffold of claim 1, wherein the reconstituted extracellular matrix is derived from cultured cells or animal tissue. (freepatentsonline.com)
  • 21. A biomaterial scaffold comprising reconstituted extracellular matrix, polyelectrolyte complex fibers and seeded cells, wherein the extracellular matrix is derived from the same or similar cell type as the seeded cells. (freepatentsonline.com)
  • It is not clear whether the extracellular matrix plays only a passive role or a more active, instructive role in shaping organs, in part, because it is difficult to measure the physical forces within densely packed cells. (elifesciences.org)
  • This work reveals that, along with providing structural support to cells, the mechanical properties of the matrix also actively guide how organs form. (elifesciences.org)
  • resistance to these forces is mediated by cells and by the extracellular matrix (ECM), including the basement membranes (BMs) that line all epithelia. (elifesciences.org)
  • Here, though, and increasingly in scientific and research circles and professionals worldwide, 'fascia' has a wider definition: all the collagenous-based soft-tissues in the body, including the cells that create and maintain that network of extra-cellular matrix (ECM). (anatomytrains.com)
  • The composition of the extracellular matrix may be a critical factor in determining the invasive potential of cancer cells, such that the production of matrix elements by glioma cells might mediate their invasion into normal tissue. (jneurosci.org)
  • Behaviors characteristic of cells in the developing brain, such as cell proliferation and migration, neuronal and glial process outgrowth, and the elaboration of the capillary network, take place in a soluble matrix that is permissive for cell movement. (jneurosci.org)
  • A model of extracellular matrix (ECM) of collagen fibers has been built, in which cells could communicate with distant partners via fiber-mediated long-range-transmitted stress states. (aps.org)
  • The Extracellular Matrices and Substrates are a group of products that provide such support for 2D and 3D cell culture research and allow for more options to provide the most in vivo -like environment for the specific cells that are being used. (peprotech.com)
  • The extra-cellualr matrix (ECM) is secreted locally and assembles into a network in the spaces surrounding cells (intercellualr space). (humpath.com)
  • The interstitial matrix is present in spaces between epithelial, endothelial, and smooth muscle cells and in connective tissue . (humpath.com)
  • The matrix helps hold the cells and tissues together and provides a means in which cell can migrate and interact. (humpath.com)
  • In vivo, cells are exposed to mechanical forces as a consequence of blood flow, interstitial flow, blood pressure, and extracellular matrix (ECM) stiffness. (sciencemag.org)
  • Matrix metalloproteinase (MMP)-1, -3 and MMP-13 expression was reduced in aging ACL but increased in degenerated ACL, mainly in the chondrocyte-like cells. (biomedsearch.com)
  • Tight junctions prevent the movement of the extracellular matrix between cells. (jiskha.com)
  • The nonliving matrix of the connective tissue through which the blood cells freely flow is the? (jiskha.com)
  • Extracellular matrix provides the microenvironment for the cells and serves as a tissue scaffold, guiding cell migration during embryonic development and wound repair. (sabiosciences.com)
  • The features of the ECM are determined both by the cells that produce the matrix and by the cells growing in it. (sabiosciences.com)
  • Cell adhesion is the binding of the cells to each other and to the extracellular matrix through cell adhesion molecules (CAMs) such as integrins, selectins, cadherins, the Ig (immunoglobulin) superfamily, and lymphocyte homing receptors. (sabiosciences.com)
  • Cancer cell invasion and metastasis is a complex, multistep process involving interactions between invading cells, the extracellular matrix, and other stromal elements ( 1 ). (aacrjournals.org)
  • Proteolytic enzymes secreted by tumor and/or host cells are required for cancer cells to invade the extracellular matrix and infiltrate lymphatic or blood vessel walls to metastasize to regional or distant sites. (aacrjournals.org)
  • It is enriched on the surface of tumor cells and stimulates the production of matrix metalloproteinases (MMPs) by adjacent stromal cells. (aacrjournals.org)
  • The collagen fibrils that these cells synthesise provide a template for postnatal growth by structure-based matrix expansion. (elifesciences.org)
  • Extracellular matrix (ECM) mechanical stiffness and its dynamic change is one of the main cues that directly affects the differentiation and proliferation of normal cells as well as the progression of disease processes such as fibrosis and cancer. (rsc.org)
  • The injured tissue environment upon sensing the stress turns on a precisely orchestrated network of immune responses by regulating cytokine-chemokine production, recruitment of immune cells, and modulating fibrogenic niche and extracellular matrix (ECM) cross-talk during fibrotic pathologies like cardiac fibrosis, liver fibrosis, laryngotracheal stenosis, systemic scleroderma, interstitial lung disease and inflammatory bowel disease. (intechopen.com)
  • Regulated turnover of extracellular matrix (ECM) is an important component of tissue homeostasis. (springer.com)
  • Many of the interactions have also been demonstrated on tissue sections or in vivo, and adherence to the extracellular matrix has been shown to promote bacterial colonization of damaged tissues. (nih.gov)
  • They orchestrate cell-cell and cell-matrix adhesive interactions from embryonic development to mature tissue function. (hindawi.com)
  • Cartilaginous ECM is remodeled continuously by a combination of production, degradation by matrix metalloproteinases (MMPs), and inhibition of MMPs activity by tissue inhibitors of MMPs [ 4 ]. (hindawi.com)
  • The metazoan extracellular matrix (ECM), an extracellular system of insoluble networks, sheets and fibrils of proteinaceous material, is a central mediator of multicellularity that is required for developmental processes, tissue organisation, homoeostasis and control of cell phenotypes throughout life. (els.net)
  • The extracellular matrix is the defining feature of connective tissue in animals. (medicalxpress.com)
  • This conference will focus on the converging roles of the extracellular matrix (ECM) and soluble factors for the repair or replacement of tissue lost or damaged due to degenerative disease, cancer, or trauma. (grc.org)
  • To isolate extracellular matrix from the kidney, place the diced cortex tissue into the beaker containing SDS solution. (jove.com)
  • Studies based on Efemp1 knock-out mouse implicate EFEMP1 function in withholding tissue integrity by stimulating the expression of Timp1 and Timp3 and inhibiting the expression and activities of matrix metalloproteinase Mmp2 , and Mmp9 (Rahn et al. (atlasgeneticsoncology.org)
  • In this educational video, Dr. Marc Gerdisch of the St. Francis Heart Center describes extracellular matrix and how it is being used to enhance tissue function in repaired mitral valves. (heart-valve-surgery.com)
  • The extracellular matrix is considered one of the defining features of connective tissue in animals. (heart-valve-surgery.com)
  • The extra-cellualr matrix (ECM) forms a significant proportion of the volume of any tissue. (humpath.com)
  • Tissue and extracellular matrix (ECM) stiffness is transduced into intracellular stiffness, signaling, and changes in cellular behavior. (sciencemag.org)
  • Preparation and Characterization of a Chitosan/Gelatin/Extracellular Matrix Scaffold and Its Application in Tissue Engineering. (sigmaaldrich.com)
  • Previous studies have demonstrated that extracellular matrix (ECM) can be used in tissue engineering due to its bioactivity. (sigmaaldrich.com)
  • Loss of flexibility is due at least in part to chemical changes in the Extracellular Matrix (ECM) in the tissue, converting a flexible network of molecules into a rigid mesh. (ddw-online.com)
  • The ability of tendons to transmit such large forces is directly attributable to an extracellular matrix (ECM) comprising collagen fibrils aligned parallel to the tissue long axis. (elifesciences.org)
  • However, the absence of a robust method of imaging cell-matrix organisation has precluded a detailed study of how the cell-rich anlage found in embryogenesis grows into a mechanically strong connective tissue in the adult. (elifesciences.org)
  • Additionally, all tissue-based neutrophilic responses to fungal infections necessitate contact with the extracellular matrix (ECM). (jimmunol.org)
  • 56% women), we related circulating concentrations of matrix metalloproteinase-9 (binary variable: detectable versus undetectable), log of tissue inhibitor of matrix metalloproteinase-1, and log of procollagen type III aminoterminal peptide (PIIINP) to incident CVD and death. (ahajournals.org)
  • Each SD increment of log of tissue inhibitor of matrix metalloproteinase-1 and log-PIIINP was associated with multivariable-adjusted hazards ratios of 1.72 (95% CI, 1.30 to 2.27) and 1.47 (95% CI, 1.11 to 1.96), respectively, for mortality risk. (ahajournals.org)
  • Conclusion- Higher circulating tissue inhibitor of matrix metalloproteinase-1 and PIIINP concentrations are associated with mortality, and higher PIIINP is associated with incident CVD, in the community. (ahajournals.org)
  • The Human Extracellular Matrix & Adhesion Molecules RT² Profiler PCR Array profiles the expression of 84 genes important for cell-cell and cell-matrix interactions. (qiagen.com)
  • The Mouse Extracellular Matrix & Adhesion Molecules RT² Profiler PCR Array profiles the expression of 84 genes important for cell-cell and cell-matrix interactions. (qiagen.com)
  • The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules. (jove.com)
  • However, the early astrocytes influence their environment not only by releasing and responding to diverse soluble factors but also express a wide range of extracellular matrix (ECM) molecules, in particular proteoglycans of the lectican family and tenascins. (frontiersin.org)
  • EFEMP1 is one member of fibulins that serve to modulate cellular behavior and functions by connecting and integrating multiple partner molecules in extracellular compartment. (atlasgeneticsoncology.org)
  • The Extracellular Matrix and Cell Adhesion PCR Arrays are designed to determine the gene expression profile of the molecules involved in cell-to-cell and cell-to-matrix interactions. (sabiosciences.com)
  • Response of basal epithelial cell surface and Cytoskeleton to solubilized extracellular matrix molecules. (rupress.org)
  • This review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the biological significance of these interactions. (nih.gov)
  • Accumulating data support the importance of interactions between pre- and postsynaptic neuronal elements with astroglial processes and extracellular matrix (ECM) for formation and plasticity of chemical synapses, and thus validate the concept of a tetrapartite synapse. (frontiersin.org)
  • This method can help answer key questions in the field of biomaterials, such as how cell matrix interactions affect sulfate. (jove.com)
  • For a basic understanding of the chemical and physical interactions of the EPS matrix, model biofilms with increasing complexity are designed and the influence of structure and composition on the physical properties is investigated. (wur.nl)
  • Finally, we determined the functional importance of integrin-fibronectin interactions and matrix metalloprotease (MMP)-facilitated cell movement during re-epithelialization and propose a model for cell locomotion during postmenstrual repair. (bioone.org)
  • Articular cartilage ECM plays a crucial role in regulating chondrocyte metabolism and functions, such as organized cytoskeleton through integrin-mediated signaling via cell-matrix interaction. (hindawi.com)
  • Thus, NF-186 assembles and links the specialized brevican-containing AIS extracellular matrix to the intracellular cytoskeleton. (rupress.org)
  • His work ties in with that of Donald Ingber on tensegrity within the cytoskeleton, and the links between the cytoskeleton and the Extra Cellular Matrix as described by James Oschman. (indigo.ca)
  • Integrins are heterodimers of α- and β - subunits that bind to the ECM through large extracellular domains and connect intracellularly to the actin cytoskeleton filaments. (sabiosciences.com)
  • I ) In the absence of extracellular signals, BMPRs and integrins do not engage without ligand binding. (portlandpress.com)
  • Subsequent binding of newly activated integrins to extracellular matrix (ECM) initiates downstream signals. (ahajournals.org)
  • Hyaluronic acid in the extracellular space confers upon tissues the ability to resist compression by providing a counteracting turgor (swelling) force by absorbing significant amounts of water. (wikipedia.org)
  • The expression of these gelatinases is relatively low in normal tissues and is induced when extracellular matrix remodeling is required. (aacrjournals.org)
  • The interstitial ECM, which makes up most of the extracellular mass of the body, consists of many different types of collagen, tenascin, proteoglycans and elastin in elastic tissues such as skin or tendon. (ddw-online.com)
  • Recent advancements in biomaterials have enabled a wide range of polymer matrices that could mimic the ECM of different tissues for a wide range of in vitro basic research and drug discovery. (rsc.org)
  • However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. (mcponline.org)
  • The podosome rosettes set the scene for global extracellular matrix degradation and internalization. (thefreedictionary.com)
  • The extracellular matrix (ECM) is a complex network of different combinations of collagens, proteoglycans, hyaluronic acid, laminin, fibronectin, and many other glycoproteins, including proteolytic enzymes involved in degradation and remodeling of the extracellular matrix. (sabiosciences.com)
  • Matrix metalloproteinases (MMP), especially MMP2, are commonly overexpressed in squamous cell carcinoma and are known to promote invasion through degradation of collagen in the basement membrane. (aacrjournals.org)
  • Matrix metalloproteinase-2 is required for the switch to the angiogenic phenotype in a tumor model," Proceedings of the National Academy of Sciences of the United States of America , vol. 97, no. 8, pp. 3884-3889, 2000. (hindawi.com)
  • Similar results were found for matrix metalloproteinase (MMP)-3 (765 vs 370 pg·mL −1 , p=0.004). (ersjournals.com)
  • The molecular mechanism of SCCRO-mediated effects on matrix metalloproteinase-2 (MMP2) levels and activity were assessed using a combination of cell biological and molecular methods, including real-time PCR, reporter assay, RNA interference, and chromatin immunoprecipitation assay. (aacrjournals.org)
  • Matrix metalloproteinase-9 was unrelated to either outcome. (ahajournals.org)
  • QIAGEN provides a broad range of assay technologies for extracellular matrix and adhesion molecule research that enable analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. (qiagen.com)
  • The resulting cell-matrix reciprocity further affected adhesion, proliferation, and migration behavior of MSCs. (mit.edu)
  • Cell adhesion mediates cell attachment, migration, and signaling to and from the extracellular matrix. (sabiosciences.com)
  • The attachment and growth of bacteria on these membranes results in formation of biofilms: to create a favourable living environment they immobilize themselves in a slimy matrix of extracellular polymeric substances (EPS). (wur.nl)
  • Down: Microscopic image of a biofilm, showing bacteria and extracellular polymeric substances. (wur.nl)
  • With immunostains, we localized collagen types I through VI, laminin, and fibronectin in frozen sections of the extracellular matrix of the prelaminar, laminar, and retrolaminar human optic nerve. (nih.gov)
  • When strain was applied on matrices containing both laminin and vitronectin, the mitogenic response to strain depended upon the vitronectin content of the matrix. (jci.org)
  • The extracellular ligands that anchor these adhesions include laminin, fibronectin, vitronectin, and various collagens. (sabiosciences.com)
  • EFEMP1 is likely to contribute the integrity of basement membrane zones and anchor other extracellular matrix structures such as elastic fibers to basement membranes. (atlasgeneticsoncology.org)
  • By establishing a Tamoxifen (Tx) inducible knockout mouse, we have demonstrated that the extracellular matrix glycoprotein fibronectin (Fn), activates critical survival signals via ß1 integrin following myocardial infarction (1). (uni-heidelberg.de)
  • Described below are the different types of proteoglycan found within the extracellular matrix. (wikipedia.org)
  • A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. (umassmed.edu)
  • L. M. Coussens and Z. Werb, Matrix metalloproteinases and the development of cancer ,, Chemistry and Biology , 3 (1996), 895. (aimsciences.org)
  • Matrix metalloproteinases (MMPs) degrade ECM components and regulate normal cell migration as well as tumor cell invasion and metastases ( 5 , 6 ). (jimmunol.org)
  • Several studies suggest that matrix metalloproteinases (MMP), a family of zinc-dependent endopeptidases, play a significant role in extracellular matrix invasion ( 3 ). (aacrjournals.org)
  • Therefore, tight extracellular and intracellular regulation of BMP signaling is critical. (portlandpress.com)
  • The internal limiting lamina of the optic nerve has an extracellular composition similar to the thicker adjacent retinal internal limiting lamina. (nih.gov)
  • The invention relates to extracellular matrix (ECM) accumulation and more particularly to composition and methods for either increasing or decreasing the same. (freepatentsonline.com)
  • Here we present a protocol to fabricate a kidney cortex extracellular matrix-derived hydrogel to retain the native kidney extracellular matrix (ECM) structural and biochemical composition. (jove.com)
  • Bosman FT, Stamenkovic I. Functional structure and composition of the extracellular matrix. (humpath.com)
  • Native extracellular matrix preserves mesenchymal stem cell 'stemness' and differentiation potential under serum-free culture conditions. (nature.com)
  • The investigators' preliminary data indicate that besides ' free ' infectious viral particles, HIV-1 infected cluster of differentiation 4 (CD4+) lymphocytes also produce extracellular viral assemblies wrapped in an extracellular matrix cocoon and tightly bound to the surface of the cell. (clinicaltrials.gov)
  • Kohn JC, Zhou DW, Bordeleau F, Zhou AL, Mason BN, Mitchell MJ, King MR, Reinhart-King CA. Cooperative effects of matrix stiffness and fluid shear stress on endothelial cell behavior. (umassmed.edu)
  • The animal extracellular matrix includes the interstitial matrix and the basement membrane . (wikipedia.org)
  • Second, I'll talk about the skin ECM, which comprises a basement membrane and interstitial matrix. (hstalks.com)
  • Extracellular matrix (ECM) exists in two forms: interstitial matrix that fills in the intercellular space and the more specialized basement membrane, which is a thin sheet of extracellular matrix underlying the epithelium. (sabiosciences.com)
  • The extracellular matrix (ECM) is an insoluble network of collagens, glycoproteins, proteoglycans and glycosaminoglycans. (els.net)
  • Extracellular matrix (ECM) contains collagen and elastic fibers embedded in a viscoelastic gel consisting of proteoglycans (PGs), hyaluronan and glycoproteins ( 29 ). (scielo.cl)
  • 4. The biomaterial scaffold of claim 2 wherein reconstituted extracellular matrix is incorporated into the polycation precursor and the polyanion precursor. (freepatentsonline.com)
  • 11. The biomaterial scaffold of claim 1, wherein the reconstituted extracellular matrix is derived from an osteoblast cell line or a hepatocarcinoma cell line. (freepatentsonline.com)
  • MAPTrix™ produces a uniform ECM surface that provides a highly controlled 2D extracellular microenvironment for cell cultures and related applications. (amsbio.com)
  • A promising tool for the in vitro exploration of extracellular microenvironment (ECM) factors that regulate cell functions is the combinatorial library approach. (amsbio.com)
  • The nerve fibers of the optic nerve are enclosed and segmented by extracellular matrix. (nih.gov)
  • The extracellular matrix consists of fibers and a basic compound. (antibodies-online.com)
  • 6 ]. It must be noted that tenocytes require a long time to produce a dense, collagen-rich neo-matrix consisting of parallel aligned type I collagen fibers. (mdpi.com)
  • wherein the matrix and the fibers are functionally associated. (freepatentsonline.com)
  • The Gordon Research Conference (GRC) on Signal Transduction by Engineered Extracellular Matrices was established in 2000 to enhance the intersection of bioengineering and cell biology with an aim to foster translation of cell-based technologies into practice. (grc.org)
  • This GRC will be held in conjunction with the "Signal Transduction by Engineered Extracellular Matrices (GRS)" Gordon Research Seminar (GRS). (grc.org)
  • Matrix, extracellular phosphoglycoprotein with ASARM motif (bone) , also known as MEPE , is a human gene . (wikidoc.org)
  • Greenhouse DG, Murphy A, Mignatti P, Zavadil J, Galloway AC, Balsam LB. Mitral valve prolapse is associated with altered extracellular matrix gene expression patterns. (umassmed.edu)
  • The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology. (springer.com)
  • 3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D ANNOUNCEMENT (Reminder) Keystone Symposium: The Extracellular Matrix of Plants: Molecular, Cellular and Developmental Biology Tamarron, Colorado. (bio.net)
  • In particular, this meeting will focus on the role that engineered matrices can play in advancing developmental biology and in vitroorganogenesis, single cell biology, microphysiological models and biofabrication ( i.e. "organs-on-chips"), regenerative medicine therapies, and immunology to study human health and disease. (grc.org)
  • Jemma Evans , Tu'uhevaha Kaitu'u-Lino , and Lois A. Salamonsen "Extracellular Matrix Dynamics in Scar-Free Endometrial Repair: Perspectives from Mouse In Vivo and Human In Vitro Studies," Biology of Reproduction 85(3), 511-523, (25 May 2011). (bioone.org)
  • The basement membrane (BM) and extracellular matrix (ECM) play critical roles in developmental and cancer biology, and are of great interest in biomathematics. (aimsciences.org)
  • 6 Division of Pulmonary, Allergy and Critical Care Medicine, Program in Protease and Matrix Biology, Gregory Fleming James Cystic Fibrosis Centre and Lung Health Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA. (sciencemag.org)
  • We aimed to characterize and determine the importance of extracellular matrix (ECM) dynamics in cell migration during endometrial repair. (bioone.org)
  • Microarray analysis revealed that in addition to modulating many of the same DC maturation genes as LPS, galectin-1 also uniquely up-regulated a significant subset of genes related to cell migration through the extracellular matrix (ECM). (jimmunol.org)
  • Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis. (biomedsearch.com)
  • Hypoxia promotes extracellular matrix (ECM) remodeling, cellular metabolic adaptation, and cancer cell metastasis. (sciencemag.org)
  • Corneal epithelium removed from underlying extracellular matrix (ECM) extends numerous cytoplasmic processes (blebs) from the formerly smooth basal surface. (rupress.org)
  • We further demonstrate in vitro production of SOM and extracellular mineralized particles in cell cultures of M. digitata . (pnas.org)
  • reported that in vitro a specialized brevican-containing matrix surrounds the AIS. (rupress.org)
  • A return to an immature, more soluble matrix during tumor growth could facilitate tumor cell motility and angiogenesis. (jneurosci.org)
  • This study examines the effect of soluble extracellular matrix components on the basal surface. (rupress.org)
  • Lethias C, Descollonges Y, Boutillon MM, Garrone R (1996) Flexilin: a new extracellular matrix glycoprotein localized on collagen fibrils. (springer.com)
  • In this minireview, we will examine key extracellular matrix and matricellular components, their changes with aging, and discuss how these alterations might influence the subsequent progression of cancer in the aged host. (wiley.com)
  • Dynamic alterations in extracellular matrix expression are essential for endometrial repair. (bioone.org)
  • Furthermore, water permeation through aquaporin-1 mediates the EV deformability, which further supports EV transport in hydrogels and a decellularized matrix. (nature.com)
  • Fig. 4: Aquaporin-1 mediates the ability of EVs to transport in engineered and decellularized matrices by increasing the EV deformability. (nature.com)
  • The brain's extracellular matrix (ECM) mediates structural stability by enwrapping synaptic contacts fundamental for long-term memory storage. (pnas.org)
  • Today I'd like to talk about several of the extracellular matrices, ECM in mammalian skin. (hstalks.com)
  • In this project we analyze effects of extracellular cues for hypertrophic remodeling and examine transcription factor circuits regulating metabolic reprogramming. (uni-heidelberg.de)
  • These structural elements form from the same mesodermal primordium, develop in similar ways, have a core leather-like matrix of collagen, and are as responsive - subject to Wolffe's Law of remodeling - to sustained forces going through the body as is the rest of the fascia. (anatomytrains.com)
  • Extracellular matrix remodeling and its contribution to protective adaptation following lengthening contractions in human muscle. (umassmed.edu)
  • 9 Matrix remodeling may therefore promote an activated EC phenotype in these regions. (ahajournals.org)
  • Background- Remodeling of the extracellular matrix (ECM) is a key aspect of myocardial response to biomechanical stress and heart failure. (ahajournals.org)
  • Objective- To evaluate if biomarkers reflecting left ventricular/vascular extracellular matrix remodeling are associated with cardiovascular disease (CVD) and death in the community. (ahajournals.org)
  • Extracellular matrix (ECM) turnover is an integral component of cardiovascular remodeling. (ahajournals.org)
  • 1. A method of treating a disease associated with extracellular matrix (ECM) accumulation in a patient, the method comprising administering to the patient a therapeutically effective amount of fibroblasts which express CD36. (freepatentsonline.com)
  • During brain maturation, the occurrence of the extracellular matrix (ECM) terminates juvenile plasticity by mediating structural stability. (pnas.org)
  • AMSBIO supplies M ussel A dhesive P rotein based ma trix ( MAPTrix TM ) recombinant extracellular matrix (ECM) that act as biometic mimics for traditional basement membrane extracts. (amsbio.com)
  • Employing optical spectroscopy we have performed a comparative study of the dielectric response of extracellular matrix and filaments of electrogenic bacteria Shewanella oneidensis MR-1, cytochrome c, and bovine serum albumin. (springer.com)
  • With the use of combinatorial libraries, one can explore the effect of numerous know and unknown factors, alone or in complex combinations in analogous to those encountered in natural extracellular microenvironments. (amsbio.com)
  • He is the editor of the recent successful book: "Extracellular Composite Matrices in Arthropods" published by Springer in 2016. (springer.com)
  • In this study, we address the possibility that CD147 could be a receptor for itself, and we identify the key extracellular domain required for CD147-dependent binding. (aacrjournals.org)