Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
The physiological renewal, repair, or replacement of tissue.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Renewal or physiological repair of damaged nerve tissue.
NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.
The largest of the three pairs of SALIVARY GLANDS. They lie on the sides of the FACE immediately below and in front of the EAR.
The channels that collect and transport the bile secretion from the BILE CANALICULI, the smallest branch of the BILIARY TRACT in the LIVER, through the bile ductules, the bile ducts out the liver, and to the GALLBLADDER for storage.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Supporting cells projecting inward from the basement membrane of SEMINIFEROUS TUBULES. They surround and nourish the developing male germ cells and secrete ANDROGEN-BINDING PROTEIN and hormones such as ANTI-MULLERIAN HORMONE. The tight junctions of Sertoli cells with the SPERMATOGONIA and SPERMATOCYTES provide a BLOOD-TESTIS BARRIER.
Neuroglial cells of the peripheral nervous system which form the insulating myelin sheaths of peripheral axons.
Endoscopic examination, therapy or surgery of the female pelvic viscera by means of an endoscope introduced into the pelvic cavity through the posterior vaginal fornix.
A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.
A drug that has been used in various urinary syndromes and as an antispasmodic. Its therapeutic usefulness and its mechanism of action are not clear. It may have local anesthetic activity and direct relaxing effects on smooth muscle as well as some activity as a muscarinic antagonist.
Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
Water swollen, rigid, 3-dimensional network of cross-linked, hydrophilic macromolecules, 20-95% water. They are used in paints, printing inks, foodstuffs, pharmaceuticals, and cosmetics. (Grant & Hackh's Chemical Dictionary, 5th ed)
Aquaporin 1 forms a water-specific channel that is constitutively expressed at the PLASMA MEMBRANE of ERYTHROCYTES and KIDNEY TUBULES, PROXIMAL. It provides these cells with a high permeability to WATER. In humans polymorphisms of this protein result in the Colton blood group antigen.
The development and use of techniques to study physical phenomena and construct structures in the nanoscale size range or smaller.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The 2nd cranial nerve which conveys visual information from the RETINA to the brain. The nerve carries the axons of the RETINAL GANGLION CELLS which sort at the OPTIC CHIASM and continue via the OPTIC TRACTS to the brain. The largest projection is to the lateral geniculate nuclei; other targets include the SUPERIOR COLLICULI and the SUPRACHIASMATIC NUCLEI. Though known as the second cranial nerve, it is considered part of the CENTRAL NERVOUS SYSTEM.
The portion of the optic nerve seen in the fundus with the ophthalmoscope. It is formed by the meeting of all the retinal ganglion cell axons as they enter the optic nerve.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.
A publication issued at stated, more or less regular, intervals.
A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.
A plastic surgical operation on the nose, either reconstructive, restorative, or cosmetic. (Dorland, 28th ed)
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
The field of biology which deals with the process of the growth and differentiation of an organism.
Conferences, conventions or formal meetings usually attended by delegates representing a special field of interest.
Process of applying for employment. It includes written application for employment or personal appearance.
A telecommunication system combining the transmission of a document scanned at a transmitter, its reconstruction at a receiving station, and its duplication there by a copier.
The showy mistletoe plant family of the order Santalales, subclass Rosidae, class Magnoliopsida. This includes parasitic tropical plants with haustoria connecting to the hosts. The leaves are opposite and thick. The flowers (4-7) have both calyx and corolla. The fruit is a berry with one seed.
Passages within the liver for the conveyance of bile. Includes right and left hepatic ducts even though these may join outside the liver to form the common hepatic duct.
Cell-cell junctions that seal adjacent epithelial cells together, preventing the passage of most dissolved molecules from one side of the epithelial sheet to the other. (Alberts et al., Molecular Biology of the Cell, 2nd ed, p22)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The biological science concerned with the life-supporting properties, functions, and processes of living organisms or their parts.
A widely expressed transmembrane glycoprotein that functions as a METASTASIS suppressor protein. It is underexpressed in a variety of human NEOPLASMS.
Pathological processes of the LIVER.
Tumors or cancer of the LIVER.

Polarized distribution of Bcr-Abl in migrating myeloid cells and co-localization of Bcr-Abl and its target proteins. (1/9343)

Bcr-Abl plays a critical role in the pathogenesis of Philadelphia chromosome-positive leukemia. Although a large number of substrates and interacting proteins of Bcr-Abl have been identified, it remains unclear whether Bcr-Abl assembles multi-protein complexes and if it does where these complexes are within cells. We have investigated the localization of Bcr-Abl in 32D myeloid cells attached to the extracellular matrix. We have found that Bcr-Abl displays a polarized distribution, colocalizing with a subset of filamentous actin at trailing portions of migrating 32D cells, and localizes on the cortical F-actin and on vesicle-like structures in resting 32D cells. Deletion of the actin binding domain of Bcr-Abl (Bcr-AbI-AD) dramatically enhances the localization of Bcr-Abl on the vesicle-like structures. These distinct localization patterns of Bcr-Abl and Bcr-Abl-AD enabled us to examine the localization of Bcr-Abl substrate and interacting proteins in relation to Bcr-Abl. We found that a subset of biochemically defined target proteins of Bcr-Abl redistributed and co-localized with Bcr-Abl on F-actin and on vesicle-like structures. The co-localization of signaling proteins with Bcr-Abl at its sites of localization supports the idea that Bcr-Abl forms a multi-protein signaling complex, while the polarized distribution and vesicle-like localization of Bcr-Abl may play a role in leukemogenesis.  (+info)

Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2. (2/9343)

Interaction between integrin alphavbeta3 and extracellular matrix is crucial for endothelial cells sprouting from capillaries and for angiogenesis. Furthermore, integrin-mediated outside-in signals co-operate with growth factor receptors to promote cell proliferation and motility. To determine a potential regulation of angiogenic inducer receptors by the integrin system, we investigated the interaction between alphavbeta3 integrin and tyrosine kinase vascular endothelial growth factor receptor-2 (VEGFR-2) in human endothelial cells. We report that tyrosine-phosphorylated VEGFR-2 co-immunoprecipitated with beta3 integrin subunit, but not with beta1 or beta5, from cells stimulated with VEGF-A165. VEGFR-2 phosphorylation and mitogenicity induced by VEGF-A165 were enhanced in cells plated on the alphavbeta3 ligand, vitronectin, compared with cells plated on the alpha5beta1 ligand, fibronectin or the alpha2beta1 ligand, collagen. BV4 anti-beta3 integrin mAb, which does not interfere with endothelial cell adhesion to vitronectin, reduced (i) the tyrosine phosphorylation of VEGFR-2; (ii) the activation of downstream transductor phosphoinositide 3-OH kinase; and (iii) biological effects triggered by VEGF-A165. These results indicate a new role for alphavbeta3 integrin in the activation of an in vitro angiogenic program in endothelial cells. Besides being the most important survival system for nascent vessels by regulating cell adhesion to matrix, alphavbeta3 integrin participates in the full activation of VEGFR-2 triggered by VEGF-A, which is an important angiogenic inducer in tumors, inflammation and tissue regeneration.  (+info)

Inhibition of transforming growth factor beta production by nitric oxide-treated chondrocytes: implications for matrix synthesis. (3/9343)

OBJECTIVE: Nitric oxide (NO) is generated copiously by articular chondrocytes activated by interleukin-1beta (IL-1beta). If NO production is blocked, much of the IL-1beta inhibition of proteoglycan synthesis is prevented. We tested the hypothesis that this inhibitory effect of NO on proteoglycan synthesis is secondary to changes in chondrocyte transforming growth factor beta (TGFbeta). METHODS: Monolayer, primary cultures of lapine articular chondrocytes and cartilage slices were studied. NO production was determined as nitrite accumulation in the medium. TGFbeta bioactivity in chondrocyte- and cartilage-conditioned medium (CM) was measured with the mink lung epithelial cell bioassay. Proteoglycan synthesis was measured as the incorporation of 35S-sodium sulfate into macromolecules separated from unincorporated label by gel filtration on PD-10 columns. RESULTS: IL-1beta increased active TGFbeta in chondrocyte CM by 12 hours; by 24 hours, significant increases in both active and latent TGFbeta were detectable. NG-monomethyl-L-arginine (L-NMA) potentiated the increase in total TGFbeta without affecting the early TGFbeta activation. IL-1beta stimulated a NO-independent, transient increase in TGFbeta3 at 24 hours; however, TGFbeta1 was not changed. When NO synthesis was inhibited with L-NMA, IL-1beta increased CM concentrations of TGFbeta1 from 24-72 hours of culture. L-arginine (10 mM) reversed the inhibitory effect of L-NMA on NO production and blocked the increases in TGFbeta1. Anti-TGFbeta1 antibody prevented the restoration of proteoglycan synthesis by chondrocytes exposed to IL-1beta + L-NMA, confirming that NO inhibition of TGFbeta1 in IL-1beta-treated chondrocytes effected, in part, the decreased proteoglycan synthesis. Furthermore, the increase in TGFbeta and proteoglycan synthesis seen with L-NMA was reversed by the NO donor S-nitroso-N-acetylpenicillamide. Similar results were seen with cartilage slices in organ culture. The autocrine increase in CM TGFbeta1 levels following prior exposure to TGFbeta1 was also blocked by NO. CONCLUSION: NO can modulate proteoglycan synthesis indirectly by decreasing the production of TGFbeta1 by chondrocytes exposed to IL-1beta. It prevents autocrine-stimulated increases in TGFbeta1, thus potentially diminishing the anabolic effects of this cytokine in chondrocytes.  (+info)

Role of thrombin receptor in breast cancer invasiveness. (4/9343)

Invasion, the ability of an epithelial cancer cell to detach from and move through a basement membrane, is a central process in tumour metastasis. Two components of invasion are proteolysis of extracellular matrix and cellular movement through it. A potential promoter of these two processes is thrombin, the serine proteinase derived from the ubiquitous plasma protein prothrombin. Thrombin promotes the invasion of MDA-MB231 breast tumour cells (a highly aggressive cell line) in an in vitro assay. Invasion by MDA-MB436 and MCF-7 cells, less aggressive cell lines, is not promoted by thrombin. Thrombin, added to the cells, is a stimulator of cellular movement; fibroblast-conditioned medium is the chemotaxin. Thrombin-promoted invasion is inhibited by hirudin. Stimulation of invasion is a receptor-mediated process that is mimicked by a thrombin receptor-activating peptide. Thrombin has no effect on chemotaxis in vitro. Thrombin receptor is detectable on the surface of MDA-MB231 cells, but not on the other two cell lines. Introduction of oestrogen receptors into MDA-MB231 cells by transfection with pHEO had no effect on thrombin receptor expression, in the presence or absence of oestradiol. This paper demonstrates that thrombin increases invasion by the aggressive breast cancer cell line MDA-MB231 by a thrombin receptor-dependent mechanism.  (+info)

Extracellular matrix remodelling in the endometrium and its possible relevance to the pathogenesis of endometriosis. (5/9343)

Essential features of endometrial physiology involve the extracellular matrix (ECM). In the pathogenesis of endometriosis, interactions of endometriosis cells with ECM can be postulated. Two systems of secreted proteases in the endometrium, the plasmin(ogen) activator/inhibitor and the matrix metalloproteinases and their inhibitors were examined in cell cultures of uterine endometrial cells from women with and without endometriosis. Soluble urokinase receptor secretion is increased, and mRNA transcription of tissue inhibitor of metalloproteinases-2 (TIMP-2) is upregulated by progestin in endometriosis. These findings are compatible with an altered ECM turnover in the endometrium of these patients that may explain a higher invasive potential of retrogradely menstruated endometrial fragments.  (+info)

Mechanisms and mediators in coal dust induced toxicity: a review. (6/9343)

Chronic inhalation of coal dust can cause several lung disorders, including simple coal workers pneumoconiosis (CWP), progressive massive fibrosis (PMF), chronic bronchitis, lung function loss, and emphysema. This review focuses on the cellular actions and interactions of key inflammatory cells and target cells in coal dust toxicity and related lung disorders, i.e. macrophages and neutrophils, epithelial cells, and fibroblasts. Factors released from or affecting these cells are outlined in separate sections, i.e. (1) reactive oxygen species (ROS) and related antioxidant protection mechanisms, and (2) cytokines, growth factors and related proteins. Furthermore, (3) components of the extracellular matrix (ECM), including the modifying role of ROS, cytokines, proteases and antiproteases are discussed in relation to tissue damage and remodelling in the respiratory tract. It is recognised that inhaled coal dust particles are important non-cellular and cellular sources of ROS in the lung, and may be significantly involved in the damage of lung target cells as well as important macromolecules including alpha-1-antitrypsin and DNA. In vitro and in vivo studies with coal dusts showed the up-regulation of important leukocyte recruiting factors, e.g. Leukotriene-B4 (LTB4), Platelet Derived Growth Factor (PDGF), Monocyte Chemotactic Protein-1 (MCP-1), and Tumor Necrosis Factor-alpha (TNF alpha), as well as the neutrophil adhesion factor Intercellular Adhesion Molecule-1 (ICAM-1). Coal dust particles are also known to stimulate the (macrophage) production of various factors with potential capacity to modulate lung cells and/or extracellular matrix, including O2-., H2O2, and NO, fibroblast chemoattractants (e.g. Transforming Growth Factor-beta (TGF beta), PDGF, and fibronectin) and a number of factors that have been shown to stimulate and/or inhibit fibroblast growth or collagen production such as (TNF alpha, TGF beta, PDGF, Insulin Like Growth Factor, and Prostaglandin-E2). Further studies are needed to clarify the in vivo kinetics and relative impact of these factors.  (+info)

Matrix valency regulates integrin-mediated lymphoid adhesion via Syk kinase. (7/9343)

Lymphocytes accumulate within the extracellular matrix (ECM) of tumor, wound, or inflammatory tissues. These tissues are largely comprised of polymerized adhesion proteins such as fibrin and fibronectin or their fragments. Nonactivated lymphoid cells attach preferentially to polymerized ECM proteins yet are unable to attach to monomeric forms or fragments of these proteins without previous activation. This adhesion event depends on the appropriate spacing of integrin adhesion sites. Adhesion of nonactivated lymphoid cells to polymeric ECM components results in activation of the antigen receptor-associated Syk kinase that accumulates in adhesion-promoting podosomes. In fact, activation of Syk by antigen or agonists, as well as expression of an activated Syk mutant in lymphoid cells, facilitates their adhesion to monomeric ECM proteins or their fragments. These results reveal a cooperative interaction between signals emanating from integrins and antigen receptors that can serve to regulate stable lymphoid cell adhesion and retention within a remodeling ECM.  (+info)

alphaSU2, an epithelial integrin that binds laminin in the sea urchin embryo. (8/9343)

At gastrulation in the sea urchin embryo dramatic cell adhesion changes contribute to primary mesenchyme cell ingression movements and to cell rearrangements during archenteron invagination. At ingression, quantitative adhesion assays demonstrated previously that primary mesenchyme cells (PMCs) change their affinity for neighboring cells, for a fibronectin-like substrate, and for the hyaline layer. To investigate the molecular basis for these and other differential cell affinities at gastrulation, we have identified an integrin that appears to be responsible for specific alterations in cell-substrate adhesion to laminin. During early cleavage stages blastomeres adhere poorly to laminin substrates. Around hatching there is a large increase in the ability of blastomeres to bind to laminin and this increase correlates temporally with the expression of an integrin on the basal surface all blastomeres. PMCs, after undergoing their epithelial-mesenchymal transition, have a strongly reduced affinity for laminin relative to ectoderm cells and, correspondingly, do not stain for the presence of the integrin. We identified the alpha integrin cDNA from Lytechinus variegatus by RT-PCR. Overlapping clones were obtained from a midgastrula cDNA library to provide a complete sequence for the integrin. The composite cDNA encoded a protein that was most similar to the alpha5 subgroup of vertebrate integrins, but there was not a definitive vertebrate integrin homolog. Northern blots and Western immunoblots showed that the sea urchin integrin, which we have named alphaSU2, is present in eggs and during all stages of development. Immunolocalization with specific polyclonal antibodies showed that alphaSU2 first appears on the basal cell surface of epithelia at the midblastula stage, at a time correlating with the increase in adhesive affinity for laminin. The protein remains at high levels on the basal surface of ectoderm cells but is temporarily reduced or eliminated from endoderm cells during their convergent-extension movements. To confirm integrin binding specificity, alphaSU2 was transfected into an alpha-integrin-deficient CHO cell line. alphaSU2-expressing CHO cells bound well to isolated sea urchin basal lamina and to purified laminin. The transfected cells bound weakly or not at all to fibronectin, type I collagen, and type IV collagen. This is consistent with the hypothesis that alphaSU2 integrin functions by binding epithelial cells to laminin in the basal lamina. In vivo, modulation of alphaSU2 integrin expression correlates with critical adhesive changes during cleavage and gastrulation. Thus, this protein appears to be an important contributor to the morphogenetic rearrangements that characterize gastrulation in the sea urchin embryo.  (+info)

TY - JOUR. T1 - Extracellular matrix organization modulates fibroblast growth and growth factor responsiveness. AU - Nakagawa, Shigenori. AU - Pawelek, Pamela. AU - Grinnell, Frederick. N1 - Funding Information: We are grateful to Drs. William Snell and George Bloom for their advice and suggestions.T his research was supported by grants from the Kendall Health Care Products Co. and the NIH (DM31321).. PY - 1989/6. Y1 - 1989/6. N2 - To learn more about the relationship between extracellular matrix organization, cell shape, and cell growth control, we studied DNA synthesis by fibroblasts in collagen gels that were either attached to culture dishes or floating in culture medium during gel contraction. After 4 days of contraction, the collagen density (initially 1.5 mg/ml) reached 22 mg/ml in attached gels and 55 mg/ml in floating gels. After contraction, attached collagen gels were well organized; collagen fibrils were aligned in the plane of cell spreading; and fibroblasts had an elongated, ...
TY - JOUR. T1 - Breast cancer cell cyclooxygenase-2 expression alters extracellular matrix structure and function and numbers of cancer associated fibroblasts. AU - Krishnamachary,Balaji. AU - Stasinopoulos,Ioannis. AU - Kakkad,Samata. AU - Penet,Marie France. AU - Jacob,Desmond. AU - Wildes,Flonne. AU - Mironchik,Yelena. AU - Pathak,Arvind P.. AU - Solaiyappan,Meiyappan. AU - Bhujwalla,Zaver M.. PY - 2017. Y1 - 2017. N2 - Cyclooxygenase-2 (COX-2) is a critically important mediator of inflammation that significantly influences tumor angiogenesis, invasion, and metastasis. We investigated the role of COX-2 expressed by triple negative breast cancer cells in altering the structure and function of the extracellular matrix (ECM). COX-2 downregulation effects on ECM structure and function were investigated using magnetic resonance imaging (MRI) and second harmonic generation (SHG) microscopy of tumors derived from triple negative MDA-MB-231 breast cancer cells, and a derived clone stably expressing a ...
Here we describe extracellular matrix alterations in footpad lesions and draining lymph nodes caused by Leishmania (L.) amazonensis in mouse strains with distinct susceptibilities to this parasite: BALB/c (susceptible), C57BL/6 (intermediate), and DBA/2 (resistant). Changes in ECM were observed mainly in BALB/c mice that, in general, presented tissue damage associated with high parasite burden. Under polarized light, Sirius Red revealed type I collagen that was predominant in the primary lesion in all strains studied at the early phase of infection, but gradually decreased and was replaced by abundant type III collagen fibre in chronic phase lesions. the presence of type III collagen seemed to provide support to inflammatory cells, mainly vacuolated and parasitized macrophages. Laminin expression was not altered during infection by L. (L.) amazonensis in any of the mouse strains studied. Furthermore, the decreased fibronectin expression, in all strains, in areas where amastigotes have been ...
Antibodies for proteins involved in regulation of extracellular matrix organization pathways, according to their Panther/Gene Ontology Classification
Antibodies for proteins involved in extracellular matrix organization pathways, according to their Panther/Gene Ontology Classification
Airway remodelling describes the histopathological changes leading to fixed airway obstruction in patients with asthma and includes extra-cellular matrix (ECM) deposition. Matrix metalloproteinase-1 (MMP-1) is present in remodelled airways but its relationship with ECM proteins and the resulting functional consequences are unknown. We used airway smooth muscle cells (ASM) and bronchial biopsies from control donors and patients with asthma to examine the regulation of MMP-1 by ECM in ASM cells and the effect of MMP-1 on ASM contraction. Collagen-I and tenascin-C induced MMP-1 protein expression, which for tenascin-C, was greater in asthma derived ASM cells. Tenascin-C induced MMP-1 expression was dependent on ERK1/2, JNK and p38 MAPK activation and attenuated by function blocking antibodies against the β1 and β3 integrin subunits. Tenascin-C and MMP-1 were not expressed in normal airways but co-localised in the ASM bundles and reticular basement membrane of patients with asthma. Further, ECM ...
This chapter introduces the matrix-mechanics formulation of quantum mechanics, emphasizing both calculational techniques and conceptual understanding. Parallels between matrix mechanics and ordinary vectors and matrices are extensively utilized. Starting with the representation of ordinary vectors as rows or columns of numbers, the scalar product is discussed, followed by the transformation of vectors by matrices, as illustrated by rotations. The vector representation of quantumstates, the inner product of two such states, and the matrix representation of operators are then introduced. The simple forms assumed in matrix mechanics by a basis state, and by an operator, when either is written in its eigenbasis, are discussed, as are the specific forms of adjoint, Hermitian, and unitary operators. The chapter concludes with a brief exposition of eigenvalue equations in matrix mechanics.
Read how fascia and extra-cellular matrix (ECM) are crucial for stability and movement. Learn to define the fascial system and ECM.
Perturbation of mechanical force at muscle attachments and its effects on tendon morphogenesis provides insights into the mechanisms underlying cellular responses to tensional force and resulting extracellular matrix production.
View Notes - BIO 320 Lec18.2009.notes from BIO 50160 at University of Texas. Lecture 18 Extracellular Matrix: Interactions between Cells and Their Environment Figure 19-3 Molecular Biology of the
Insulin-like growth factor (IGF)-I binds to the ECM protein vitronectin (VN) through IGF binding proteins (IGFBPs) to enhance proliferation and migration of skin keratinocytes and fibroblasts. Although evidence exists for the role of individual components of the complex (IGF-I, IGFBP-3 and VN), the cellular functions stimulated by these proteins together as a complex remains un-investigated in melanoma cells. We report here that the IGF-I:IGFBP-3:VN trimeric complex stimulates a dose-dependent increase in the proliferation and migration of WM35 and Sk-MEL28 melanoma cells. In 3D Matrigel™ and hydrogel cultures, both cell lines formed primary tumor-like spheroids, which increased in size in a dose-dependent manner in response to the trimeric complex. Furthermore, we reveal IGFBP-3:VN protein complexes in malignant melanoma and squamous cell carcinoma patient tissues, where the IGFBP-3:VN complex was seen to be predominantly tumor cell-associated. Peptide antagonists designed to target the ...
N-acetyl proline-glycine-proline (ac-PGP) is a matrix-derived chemokine produced through the proteolytic destruction of collagen by matrix metalloproteinases (MMPs). While upregulation and activation of MMPs and concomitant degradation of the extracellular matrix are known to be associated with neurological injury in ischemic stroke, the production of ac-PGP in stroke brain and its effects on neurons have not been investigated. We examined the effects of ac-PGP on primary cortical neurons and found that it binds neuronal CXCR2 receptors, activates extracellular signal-regulated kinase 1/2 (ERK1/2), and induces apoptosis associated with caspase-3 cleavage in a dose-dependent manner. After transient ischemic stroke in rats, ac-PGP was significantly upregulated in infarcted brain tissue. The production of ac-PGP in brain in ischemia/reperfusion injury and its propensity to induce apoptosis in neurons may link MMP-mediated destruction of the extracellular matrix and opening of the blood-brain ...
Post-Doctoral Fellow, *Faculty of Dentistry, University of Toronto, *Lunenfeld Tanenbaum Research Institute, Mt. Sinai Hospital, Toronto, *Department of Laboratory Medicine & Pathobiology, Faculty of Medicine, University of Toronto ...
We recently showed that differential expression of extracellular matrix (ECM) genes delineates four subgroups of breast carcinomas (ECM1, -2, -3- and -4) with different clinical outcome. To further investigate the characteristics of ECM signature and its impact on tumor progression, we conducted unsupervised clustering analyses in 6 additional independent datasets of invasive breast tumors from different platforms for a total of 643 samples. Use of four different clustering algorithms identified ECM3 tumors as an independent group in all datasets tested. ECM3 showed a homogeneous gene pattern, consisting of 58 genes encoding 43 structural ECM proteins. From 26 to 41% of the cases were ECM3-enriched, and analysis of datasets relevant to gene expression in neoplastic or corresponding stromal cells showed that both stromal and breast carcinoma cells can coordinately express ECM3 genes. In in vitro experiments, β-estradiol induced ECM3 gene production in ER-positive breast carcinoma cell lines, ...
Purpose: : We previously found that IGF-I, TGF-β, and PDGF, but not FGF-2, stimulate collagen synthesis by keratocytes in culture. We also found that culturing insulin activated keratocytes under a thin layer of agarose increases the processing of procollagen to collagen and increases ECM formation (PMID: 18938157). We now evaluate the ECM formed by keratocytes cultured in these growth factors and under agarose. Methods: : Collagenase-isolated keratocytes from bovine corneas were plated at 40,000 cells/cm2 and then cultured with DMEM/F12 alone, or DMEM/F12 supplemented with either 10ng IGF-I, 2ng TGF-β, 10ng FGF-2, or 10ng PDGF/ml, all with ascorbate. Cultures were overlayed with ~1mm of 3% agarose on day 4 and harvested for analysis on day 12. Keratocytes cell number was determined by measuring DNA content (cyquant assay). Collagen was determined by pepsin digestion, SDS/PAGE, simply blue staining, and by western blots with antibodies to procollagen I and III. ECM morphology was evaluated by ...
Introduction: Changes in ventricular extracellular matrix (ECM) composition of hypertrophic cardiomyopathy determine clinical outcomes. The effects of MSC transplantation upon ventricular remodeling and determinants of ECM composition in hypertrophic cardiomyopathy have not been studied.. Hypothesis: We hypothesized that MSC therapy has beneficial effects upon ventricular remodeling and ECM proteases and tissue inhibitors in a rat model of pressure overload cardiomyopathy.. Methods: Sprague-Dawley rats underwent aortic banding and were followed by echocardiography for development of heart failure. After a decrease in fractional shortening of 25% from baseline, intra-coronary randomized injection of 1 x 106 MSC (n=28) or PBS (n=20) was performed. Serial echocardiography was performed to identify reverse remodeling. Left ventricular protein analysis including matrix metalloproteinases (MMP-2, 3, 6 and 9) and tissue inhibitors of metalloproteinases (TIMP-1, 2 and 3) was performed after sacrifice on ...
Limitations associated with demineralised bone matrix and other grafting materials have motivated the development of alternative strategies to enhance the repair of large bone defects. The growth plate (GP) of developing limbs contain a plethora of growth factors and matrix cues which contribute to long bone growth, suggesting that biomaterials derived from its extracellular matrix (ECM) may be uniquely suited to promoting bone regeneration. The goal of this study was to generate porous scaffolds from decellularised GP ECM and to evaluate their ability to enhance host mediated bone regeneration following their implantation into critically-sized rat cranial defects. The scaffolds were first assessed by culturing with primary human macrophages, which demonstrated that decellularisation resulted in reduced IL-1β and IL-8 production. In vitro, GP derived scaffolds were found capable of supporting osteogenesis of mesenchymal stem cells via either an intramembranous or an endochondral pathway, demonstrating
TY - JOUR. T1 - Cardiac extracellular matrix remodeling: Fibrillar collagens and Secreted Protein Acidic and Rich in Cysteine (SPARC). AU - McCurdy, Sarah. AU - Baicu, Catalin F.. AU - Heymans, Stephane. AU - Bradshaw, Amy D.. PY - 2010/3. Y1 - 2010/3. KW - BM-40. KW - Osteonectin. KW - SPARC. KW - Extracellular matrix. KW - Remodeling. KW - Review. U2 - 10.1016/j.yjmcc.2009.06.018. DO - 10.1016/j.yjmcc.2009.06.018. M3 - Article. C2 - 19577572. VL - 48. SP - 544. EP - 549. JO - Journal of Molecular and Cellular Cardiology. JF - Journal of Molecular and Cellular Cardiology. SN - 0022-2828. IS - 3. ER - ...
Cognitive impairment associated with MDD has been well characterized (33-35). This includes deficits in declarative and spatial memory (36, 37), supporting a role for hippocampus-mediated dysfunction and other related (endo)phenotypes, for example, decreased hippocampal volume, in MDD (38). However, the molecular mechanisms underlying this association remain to be elucidated. Here, we used a preclinical rat model that induces several long-lasting depressive-like behaviors (11, 12) to investigate the connection between hippocampal pathology and cognitive deficits. Our data indicate a causal relationship between aberrant synaptic CSPG expression, alterations in the number of PNNs, and dysregulation of the hippocampal network that, together, mediate cognitive impairments in our rat model.. Collectively, our data highlight the dorsal hippocampus as a principal mediator of cognitive deficits in the SDPS paradigm. At the behavioral level, SDPS impaired short-term object location memory, as assessed by ...
TY - JOUR. T1 - Differential matrix rigidity response in breast cancer cell lines correlates with the tissue tropism. AU - Kostic, Ana. AU - Lynch, Christopher D.. AU - Sheetz, Michael. PY - 2009/7/23. Y1 - 2009/7/23. N2 - Metastasis to a variety of distant organs, such as lung, brain, bone, and liver, is a leading cause of mortality in the breast cancer patients. The tissue tropism of breast cancer metastasis has been recognized and studied extensively, but the cellular processes underlying this phenomenon, remain elusive. Modern technologies have enabled the discovery of a number of the genetic factors determining tissue tropism of malignant cells. However, the effect of these genetic differences on the cell motility and invasiveness is poorly understood. Here, we report that cellular responses to the mechanical rigidity of the extracellular matrix correlate with the rigidity of the target tissue. We tested a series of single cell populations isolated from MDA-MB-231 breast cancer cell line in ...
Fibrotic cardiac disease, a leading cause of death worldwide, manifests as substantial loss of function following maladaptive tissue remodeling. Fibrosis can affect both the heart valves and the myocardium and is characterized by the activation of fibroblasts and accumulation of extracellular matrix. Valvular interstitial cells and cardiac fibroblasts, the cell types responsible for maintenance of cardiac extracellular matrix, are sensitive to changing mechanical environments, and their ability to sense and respond to mechanical forces determines both normal development and the progression of disease. Recent studies have uncovered specific adhesion proteins and mechano-sensitive signaling pathways that contribute to the progression of fibrosis. Integrins form adhesions with the extracellular matrix, and respond to changes in substrate stiffness and extracellular matrix composition. Cadherins mechanically link neighboring cells and are likely to contribute to fibrotic disease propagation. ...
Sigma-Aldrich offers abstracts and full-text articles by [Hanne Haslene-Hox, Eystein Oveland, Kathrine Woie, Helga B Salvesen, Olav Tenstad, Helge Wiig].
a cytonaute of molecular size traveling toward a cell, before reaching the plasma membrane, would first need to go through a jungle of stems, branches, rain forest vines, and lianas. In tissues, this messy tangle is the extracellular matrix. Extracellular matrix is a scaffold of proteins and carbohydrates located around the cells that is synthesized by the cells themselves. Some authors duggest that this definition only applies to the insoluble components of the extracellular matrix. Extracellular matrix was invented by multicellular organisms. It was needed to keep cells together by adhesion, and therefore tissues appeared. During evolution, extracellular matrix got many other functions, not just adhesion, such as being responsible for the mechanical properties of most tissues (both in plant and animals), keeping cell morphology, allowing cell communication, setting pathways for cell migration, modulating cell differentiation and physiology, keeping growth factors in some places, and many ...
Sigma-Aldrich offers abstracts and full-text articles by [Samantha D Smith, Ruhul H Choudhury, Patricia Matos, James A Horn, Stephen J Lye, Caroline E Dunk, John D Aplin, Rebecca L Jones, Lynda K Harris].
1. Dickstein K, Cohen-Solal A, Filippatos G, McMurray JJ, Ponikowski P, Poole-Wilson PA. et al. ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure 2008: the Task Force for the Diagnosis and Treatment of Acute and Chronic Heart Failure 2008 of the European Society of Cardiology. Developed in collaboration with the Heart Failure Association of the ESC (HFA) and endorsed by the European Society of Intensive Care Medicine (ESICM). Eur Heart J. 2008;29:2388-442 2. Graham HK, Horn M, Trafford AW. Extracellular matrix profiles in the progression to heart failure. European Young Physiologists Symposium Keynote Lecture-Bratislava 2007. Acta Physiol (Oxf). 2008;194:3-21 3. Yamazaki T, Lee JD, Shimizu H, Uzui H, Ueda T. Circulating matrix metalloproteinase-2 is elevated in patients with congestive heart failure. Eur J Heart Fail. 2004;6:41-5 4. George J, Patal S, Wexler D, Roth A, Sheps D, Keren G. Circulating matrix metalloproteinase-2 but not matrix metalloproteinase-3, ...
Trappmann, B and Gautrot, JE and Connelly, JT and Strange, DG and Li, Y and Oyen, ML and Cohen Stuart, MA and Boehm, H and Li, B and Vogel, V and Spatz, JP and Watt, FM and Huck, WT (2012) Extracellular-matrix tethering regulates stem-cell fate. Nat Mater, 11. 742-. ISSN 1476-1122. Full text not available from this repository ...
Significant progress has been achieved toward elucidating the molecular mechanisms that underlie breast cancer progression; yet, much less is known about the associated cellular biophysical traits. To this end, we use time-lapsed confocal microscopy to investigate the interplay among cell motility, three-dimensional (3D) matrix stiffness, matrix architecture, and transforming potential in a mammary epithelial cell (MEC) cancer progression series. We use a well characterized breast cancer progression model where human-derived MCF10A MECs overexpress either ErbB2, 14-3-3ζ, or both ErbB2 and 14-3-3ζ, with empty vector as a control. Cell motility assays showed that MECs overexpressing ErbB2 alone exhibited notably high migration speeds when cultured atop two-dimensional (2D) matrices, while overexpression of 14-3-3ζ alone most suppressed migration atop 2D matrices (as compared to non-transformed MECs). Our results also suggest that co-overexpression of the 14-3-3ζ and ErbB2 proteins facilitates ...
Mathematical Institute, Leiden University, The Netherlands. Title: Stigmergy in blood vessel growth: how indirect mechanical and chemical signaling, via the extra-cellular matrix, can coordinate collective cell behavior. Abstract: Angiogenesis, the formation of new blood vessels sprouting from existing vessel, occurs in several situations like wound healing, tissue remodeling, and near growing tumors. Under hypoxic conditions, tumor cells secrete growth factors, including VEGF. VEGF activates endothelial cells (ECs) in nearby vessels, leading to the migration of ECs out of the vessel and the formation of growing sprouts. A key process in angiogenesis is cellular self-organization, and previous modeling studies have identified mechanisms for producing networks and sprouts. Most theoretical studies of cellular self-organization during angiogenesis have ignored the interactions of ECs with the extra-cellular matrix (ECM), the jelly or hard materials that cells live in. Apart from providing ...
S. and Pederson, T. (1973) Biochem. 72,2766-2773. ; and Kim, J. (1974) Arch. Bioch. 767,1-10. E. (1964) P. NAS US 52,93-100. H. 251-276, (L. , Amsterdam. E. (1963) P. NAS US 50,1026-1032. ; and Harbers, E. (1973) Bioc. Biop. A. 557,295-304. A. (1972) P. NAS US 69,3417-3421. ; and Stambaugh, R. (1974) Arch. Bioch. 767,11-19. Y. and Bonner, J. (1970) J. Mol. Biol. 45,469-487. H. L. (1962) J. Mol. Biol. 5,172-184. J. (1974) Science 755,817-824. 34 EXTRACELLULAR MATRIX INFLUENCES ON GENE EXPRESSION 24. REFERENCES 1. Grobstein, C. (1954) Tissue interaction in the morphogenesis of mouse embryonic rudiments in vitro. In Aspects of Synthesis and Order in Growth, pp. 233-256, Princeton Univ. Press, Princeton, New Jersey. 2. Grobstein, C. (1948) Optimum gonopodial morphogenesis in Platypoecilus maculatus with constant dosage of methyl testosterone. J. Exp. Zool. 109:215-237. 3. Weiss, P. (1947) The problem of specificity in growth and development. Yale J. Biol. and Med. 19:235-278. 4. Grobstein, C. ...
Here researchers employ three-dimensional culture systems for conditional gene targeted primary mouse embryonic fibroblasts that better simulate the reciprocal and adaptive interactions between cells and surrounding matrix, to define the role of Cdc42 signaling pathways in extracellular matrix organization. [J Biol Chem] Abstract ...
Unitary surgical devices (10) are disclosed. One group of the illustrated devices has a pair of biocompatible, bioresorbable anchors (16,18) connected to fixed lengths suture. The anchors (16,18) and fixed length of suture are connected to each other prior to surgery. Another group of unitary surgical devices has a pair of fixating mechanisms (15,17) connected to a base (21) prior to surgery. The second group of illustrated devices generally includes extracellular matrix material either as part of the base (21) or supported on the base (21). The extracellular matrix material serves as tissue regenerating material. In the second group of unitary surgical devices, the fixating mechanisms illustrated generally comprise suture, anchors or pre-formed holes in the base. All of the illustrated unitary surgical devices are useful in repairing a damaged meniscus. The first group of unitary surgical devices can be used to approximate inner surfaces of a tear in the meniscus. The second group of devices can be
Fingerprint Dive into the research topics of Dual-site recognition of different extracellular matrix components by anti-angiogenic/neurotrophic serpin, PEDF. Together they form a unique fingerprint. ...
This is not the fifth installment of the Matrix series, but in some senses the Extracellular Matrix is truly fundamental to our existence. The Extracellular Matrix is the vital fluid around our cells in each of our tissues. This matrix constantly changes, remodels, activates, degrades and repairs its components. In many tissues its responsible for laying down collagen and elastin. The matrix has to have balance between synthesis and degradation by matrix metalloproteinases (MMPs), which are further controlled by activators and tissue inhibitors of MMPs (which is why we all use our Alastin products, more of that in another post!). Remodeling of the matrix, both the activation and the destruction to make way for the new are regulated growth factors.. Growth factors have to work through integrins are transmembrane receptors that facilitate cell-extracellular matrix (ECM) adhesion, and regulate the cells to perform their functions. In the vagina the cells that have heathy are fibroblasts. Vaginal ...
Synonyms for Extracellular matrix in Free Thesaurus. Antonyms for Extracellular matrix. 1 antonym for extracellular: intracellular. What are synonyms for Extracellular matrix?
Abstract The formation and remodeling of the embryonic valves is a complex and dynamic process that occurs within a constantly changing hemodynamic environment. Defects in embryonic and fetal valve remodeling are the leading cause of congenital heart defects, yet very little is known about how fibrous leaflet tissue is created from amorphous gelatinous masses called cushions. Microenvironmental cues such as mechanical forces and extracellular matrix composition play major roles in cell differentiation, but almost all research efforts in valvulogenesis center around genetics and molecular approaches. This review summarizes what is known about the dynamic mechanical and extracellular matrix microenvironment of the atrioventricular and semilunar valves during embryonic development and their possible guidance roles. A variety of new computational tools and sophisticated experimental techniques are progressing that enable precise microenvironmental alterations that are critical to complement genetic gain and
When DArcy Wentworth Thompsons On Growth and Form was published 100 years ago, it raised the question of how biological forms arise during development and across evolution. In light of the advances in molecular and cellular biology since then, a succinct modern view of the question states: how do genes encode geometry? Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales.. Read the Editorial by guest editors Thomas Lecuit and L. Mahadevan, as they provide a perspective on the influence of DArcy Thompsons work and an overview of the articles in this issue.. ...
Exhibits extracellular matrix binding activity. Involved in cell adhesion and extracellular matrix organization. Localizes to extracellular matrix and extracellular space. Is expressed in several structures, including central nervous system; genitourinary system; heart and pericardium; sensory organ; and skeleton. Used to study corneal dystrophy. Human ortholog(s) of this gene implicated in corneal dystrophy (multiple). Orthologous to human TGFBI (transforming growth factor beta induced ...
Elevations in myocardial stress initiate structural remodeling of the heart in an attempt to normalize the imposed stress. This remodeling consists of cardiomyocyte hypertrophy and changes in the amount of collagen, collagen phenotype and collagen cross-linking. Since fibrillar collagen is a relativ …
|h6>Highlights|/h6> |ul> |li>miRNA expression is altered in cancer, often through aberrant methylation |/li> |li>Altered expression of miRNAs that regulate extracellular matrix gene expression is associated with development of metastatic cancer |/li> |li>Novel approaches exploiting miRNA technology will aid in development of new treatments and diagnostic tools|/li> |/ul>
The present invention relates to an apparatus and a method for sealing a puncture in a tubular tissue structure or the wall of a body cavity. More specifically, the present invention is directed to an apparatus and method for sealing a puncture site in the wall of a tubular tissue structure, or in the wall of a body cavity with submucosal tissue or another extracellular or matrix-derived tissue capable of remodeling endogenous connective tissue in vivo. The submucosal tissue or another extracellular matrix-derived tissue is inserted into the puncture site as a sheet on an introducer element such as a needle, a cannula, a guide wire, an introducer element adapted for dialysis, an introducer element adapted for catheterization, a trocar, or any other introducer element used to access the lumen of a tubular tissue structure or used to access a body cavity.
Patrick McGean, director of the Cellular Matrix Study, talks about the health benefits of sulfur . How to choose the right supplement and more on ORN.
Extracellular matrix provides the microenvironment for the cells and serves as a tissue scaffold, guiding cell migration during embryonic development and wound repair. Beyond that, it also functions as the repository and modulator of growth factors and cytokines, and therefore is responsible for transmitting environmental signals to the cells.. Among proteases, the matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family are often associated with ECM degradation and remodeling. The inhibitors of MMPs are called tissue inhibitors of metalloproteinases (TIMPs), which are comprised of TIMP-1, TIMP-2, TIMP-3, and TIMP-4. The interactions between these proteases and their inhibitors play important roles in cell morphogenesis, angiogenesis, tissue remodeling, tissue repair, tumor metastasis, cirrhosis, and arthritis. The features of the ECM are determined both by the cells that produce the matrix and by the cells growing in it. QIAGENs ...
Aging is a major risk factor for cardiovascular disease. Although the impact of aging has been extensively studied, little is known regarding the aging processes in cells of the heart. Here we analyzed the transcriptomes of hearts of 12-week-old and 18-month-old mice by single-nucleus RNA-sequencing. Among all cell types, aged fibroblasts showed most significant differential gene expression, increased RNA dynamics, and network entropy. Aged fibroblasts exhibited significantly changed expression patterns of inflammatory, extracellular matrix organization angiogenesis, and osteogenic genes. Functional analyses indicated deterioration of paracrine signatures between fibroblasts and endothelial cells in old hearts. Aged heart-derived fibroblasts had impaired endothelial cell angiogenesis and autophagy and augmented proinflammatory response. In particular, expression of Serpine1 and Serpine2 were significantly increased and secreted by old fibroblasts to exert antiangiogenic effects on endothelial ...
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Matrix metalloproteinases (MMPs) are now acknowledged as key players in the regulation of both cell-cell and cell-extracellular matrix interactions. They are involved in modifying matrix structure, growth factor availability and the function of cell surface signalling systems, with consequent effects on cellular differentiation, proliferation and apoptosis. They play central roles in morphogenesis, wound healing, tissue repair and remodelling in response to injury and in the progression of diseases such as arthritis, cancer and cardiovascular disease. Because of their wide spectrum of activities and expression sites, the elucidation of their potential as drug targets in disease or as important features of the repair process will be dependent upon careful analysis of their role in different cellular locations and at different disease stages. Novel approaches to the specific regulation of individual MMPs in different contexts are also being developed.
Matrix metalloproteinases (MMPs) are now acknowledged as key players in the regulation of both cell-cell and cell-extracellular matrix interactions. They are involved in modifying matrix structure, growth factor availability and the function of cell surface signalling systems, with consequent effects on cellular differentiation, proliferation and apoptosis. They play central roles in morphogenesis, wound healing, tissue repair and remodelling in response to injury and in the progression of diseases such as arthritis, cancer and cardiovascular disease. Because of their wide spectrum of activities and expression sites, the elucidation of their potential as drug targets in disease or as important features of the repair process will be dependent upon careful analysis of their role in different cellular locations and at different disease stages. Novel approaches to the specific regulation of individual MMPs in different contexts are also being developed.
Atherosclerosis is an inflammatory disease in which endothelial cell (EC) activation leads to leukocyte recruitment into artery walls, followed by formation of plaques containing lipid-laden macrophages and smooth muscle cells.1 Plaques can occlude vessels and cause ischemia, or rupture to cause stroke or myocardial infarction. Whereas systemic risk factors such as hypertension, smoking, and obesity play important roles in atherogenesis, plaques show a predilection for vessel branch points and regions of high curvature, where flow is low and shows a variety of complex patterns that are grouped together under the term disturbed flow.2 These areas show increased EC turnover, altered redox regulation, and upregulation of proinflammatory genes that contribute to atherosclerotic progression.3,4 By contrast, areas of high laminar shear show downregulation of proatherogenic genes and upregulation of atheroprotective genes and are resistant to atherosclerosis.5. In vitro, acute application of laminar ...
NPL scientists have created a functional model of the native extracellular matrix which provides structural support to cells to aid growth and proliferation and could lead to advances in regenerative medicine.
All vascular implants, including stents, heart valves and graft materials exhibit suboptimal biocompatibility that significantly reduces their clinical efficacy. A range of biomolecules in the subendothelial space have been shown to play critical roles in local regulation of thrombosis, endothelial growth and smooth muscle cell proliferation, making these attractive candidates for modulation of vascular device biointegration. However, classically used biomaterial coatings, such as fibronectin and laminin, modulate only one of these components; enhancing endothelial cell attachment, but also activating platelets and triggering thrombosis. This review examines a subset of extracellular matrix molecules that have demonstrated multi-faceted vascular compatibility and accordingly are promising candidates to improve the biointegration of vascular biomaterials.
Understanding biological structures toward nature-inspired design is an important field of research (1-5). Over the past several decades, numerous studies had been carried out to identify the scientific basis for the naturally adaptive growth of biological structures through environmental exposure of biomaterials and continuous optimization of the response of these biomaterials at various geometric length scales to satisfy specific environmental conditions (2, 6-11). A significant advancement has been made in recent years in this field of science, and it has led to important applications in defense and safety, automotive, and architecture (9-11). Several groups of researchers have reported detailed reviews summarizing these advancements (2, 6-12). Most biological materials consist of fascinating structure at micron and submicron scales (2, 6-17). Examples are the organization of nanoscale collagen fibrils in extracellular matrix structures, the two-level hierarchy of open cell structure in ...
Cell / extracellular matrix signaling[edit]. The extracellular matrix is composed of glycoproteins (proteins and ... In biology, juxtacrine signalling (or contact-dependent signalling) is a type of cell / cell or cell / extracellular matrix ... An extracellular matrix glycoprotein and a membrane protein interact.. Additionally, in unicellular organisms such as bacteria ... Cell adhesion, mechanical adhesion between cells and/or the extracellular matrix. *Role of cell adhesions in neural development ...
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Extracellular matrix. *Matrix gla protein. This article incorporates text from the public domain Pfam and InterPro IPR001393 ...
extracellular region. • elastic fiber. • extracellular matrix. • collagen-containing extracellular matrix. Biological process. ... extracellular matrix structural constituent. • protein binding. Cellular component. • proteinaceous extracellular matrix. • ... extracellular matrix disassembly. • extracellular matrix organization. • blood circulation. • respiratory gaseous exchange. ... into elastin fibres immediately after their synthesis by the cell and during their export into the extracellular matrix. ...
The roles of such proteins include protection and support, forming connective tissue, tendons, bone matrices, and muscle fiber ... Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
extracellular exosome. • basal lamina. • extracellular matrix. • collagen-containing extracellular matrix. Biological process. ... extracellular matrix structural constituent. • protein binding. Cellular component. • proteinaceous extracellular matrix. • ... extracellular matrix organization. • regulation of cell migration. • regulation of embryonic development. • negative regulation ... Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They ...
extracellular matrix Reticular fibers. Form a scaffolding for other cells. Type III collagen. liver, bone marrow, and lymphatic ... Both the ground substance and proteins (fibers) create the matrix for CT. Connective tissues are derived from the mesenchyme. ... Cells are spread through an extracellular fluid.. *Ground substance - A clear, colorless, and viscous fluid containing ...
Extracellular matrix. Collagen. Fibril forming. *type I *COL1A1. *COL1A2. *type II (COL2A1) ...
extracellular matrix binding. Компонент клетки. • proteinaceous extracellular matrix. • cytoplasm. • integral component of ... Tomkowicz B., Rybinski K., Foley B., et al. Interaction of endosialin/TEM1 with extracellular matrix proteins mediates cell ... extracellular vesicular exosome. Биологический процесс. • biological_process. • positive regulation of cell proliferation. • ...
During chemotaxis, cell movement is facilitated by the binding of β1 integrins to components of the extracellular matrix: VLA-3 ... Sorokin L (October 2010). "The impact of the extracellular matrix on inflammation". Nature Reviews. Immunology. Nature ... VLA-4 and VLA-5 to fibronectin and VLA-2 and VLA-3 to collagen and other extracellular matrix components. ... Cellular activation via extracellular chemokines causes pre-formed β2 integrins to be released from cellular stores. Integrin ...
Flaim, Christopher J; Chien, Shu; Bhatia, Sangeeta N (2005). "An extracellular matrix microarray for probing cellular ... Cell micropatterning can be done using microcontact patterning of extracellular matrix proteins, cellular electrophoresis, ... Extracellular microelectrodes have been patterned onto an inflatable helix-shaped plastic in cochlear implants to improve ... or fabricating the microneedles with drug and coating matrix for maximum drug loading. Microneedles for interstitial fluid ...
They proposed a class of emergency response molecules which are released from the extracellular matrix at the site of an injury ... "Matrikines in the regulation of extracellular matrix degradation". Biochimie. 87 (3-4): 353-60. doi:10.1016/j.biochi.2004.10. ... The small size of GHK permits speedy traveling in extracellular space and its easy access to cellular receptors. The molecular ... "The tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+ stimulates matrix metalloproteinase-2 expression by fibroblast ...
... and localised proteolysis are mediated mainly by matrix metalloproteases (MMPs). Degradation of the extracellular matrix begins ... The primary extracellular matrix components and cell-surface receptors which aid in metastasis are: Integrin signalling ... TNC is an adhesion-modulating extracellular matrix glycoprotein. It is highly expressed in tumor stroma and stimulates tumor- ... or extracellular matrix) of BMFs. Inactive MMP-2 present on the surface of BMFs is displaced by breast-cancer cells. Cancer ...
Extracellular Matrix Biochemistry. Elsevier, New York, 1984. Reddi A.H. (Ed.), Extracellular Matrix: Structure and Function. A. ... Homology of bone-inductive proteins from human, monkey, bovine, and rat extracellular matrix. Proc Natl Acad Sci U S A 1983; 80 ... The Reddi laboratory has also made important discoveries unraveling the role of the extracellular matrix in bone and cartilage ... They demonstrated first that BMPs bind the extracellular matrix, are present at the apical ectodermal ridge in the developing ...
... this makes plasma the extracellular matrix of blood cells. It makes up about 55% of the body's total blood volume.[1] It is the ... intravascular fluid part of extracellular fluid (all body fluid outside cells). It is mostly water (up to 95% by volume), and ...
extracellular matrix. • proteinaceous extracellular matrix. • basement membrane. • extracellular exosome. • intracellular. • ... extracellular matrix disassembly. • extracellular matrix organization. • heart development. • camera-type eye development. • ... extracellular matrix constituent conferring elasticity. • protein complex binding. • extracellular matrix structural ... sequestering of BMP in extracellular matrix. • sequestering of TGFbeta in extracellular matrix. • negative regulation of ...
The extracellular matrix is the key to generating entire organs in vitro. It was found that by carefully removing the cells of ... 2015). "Extracellular Matrix as a Driver for Lung Regeneration". Annals of Biomedical Engineering. 43: 568-576. doi:10.1007/ ... Alan Spievack, was researching regeneration and provided him with powdered extracellular matrix, developed by Dr. Stephen ...
Extracellular matrix Bert JL; Pearce RH (1984). The interstitium and microvascular exchange. In: Handbook of Physiology. The ... The interstitial compartment is composed of connective and supporting tissues within the body - called the extracellular matrix ...
Borrelia burgdorferi and the extracellular matrix". Trends in Microbiology. 15 (8): 350-4. doi:10.1016/j.tim.2007.06.003. PMID ... and hiding in the extracellular matrix, which may interfere with the function of immune factors.[89][90] ...
Tricuspid Valve Replacement with Extracellular Matrix Sleeve for Ebstein's Anomaly 2012 Burke at Miami Children's Hospital ...
extracellular matrix. • proteinaceous extracellular matrix. • fibrillar collagen trimer. • lysosomal lumen. • Golgi lumen. • ... extracellular exosome. • extracellular space. • extracellular region. Biological process. • response to organic cyclic compound ... Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12.[5] ... Lumican is present in the extracellular matrix of uteral tissues in fertile women.[16] There is an increase of lumican during ...
extracellular matrix disassembly. • extracellular matrix organization. • odontogenesis of dentin-containing tooth. • cell ... Basigin (BSG) also known as extracellular matrix metalloproteinase inducer (EMMPRIN) or cluster of differentiation 147 (CD147) ... 2002). "Extracellular matrix metalloproteinase inducer (EMMPRIN) is induced upon monocyte differentiation and is expressed in ... 1996). "Human keratinocytes express EMMPRIN, an extracellular matrix metalloproteinase inducer". J. Invest. Dermatol. 106 (6): ...
Arias, J. L.; Fernandez, M. S. (2001). "Role of extracellular matrix molecules in shell formation and structure". World's ... The chicken eggshell is 95[citation needed]-97%[2] calcium carbonate crystals, which are stabilized by a protein matrix.[3][4][ ... While the bulk of eggshell is made of calcium carbonate, it is now thought that the protein matrix has an important role to ... Typically, eggshells were used as biofiller in polyaniline matrix to detect ammonia gas. The optimum ratio between eggshells ...
Kühn, Klaus (1997). "Extracellular matrix constituents as integrin ligands". In Elbe, Johannes A. (ed.). Integrin-ligand ... Laminins are high-molecular weight (~400 to ~900 kDa) proteins of the extracellular matrix. They are a major component of the ... The trimeric proteins intersect to form a cross-like structure that can bind to other cell membrane and extracellular matrix ... M. A. Haralson; John R. Hassell (1995). Extracellular matrix: a practical approach. Ithaca, N.Y: IRL Press. ISBN 978-0-19- ...
... s are extracellular matrix glycoproteins. They are abundant in the extracellular matrix of developing vertebrate ... One mechanism to explain this may come from its ability to bind to the extracellular matrix glycoprotein fibronectin and block ...
... extracellular matrix glycoproteins. Vertebrate Emu proteins, which could interact with several different extracellular matrix ... Proteins known to contain an EMI domain include: Vertebrate Emilins, extracellular matrix glycoproteins. Vertebrate Multimerins ... The EMI domain is most often found at the N terminus of metazoan extracellular proteins that are forming or are compatible with ... a novel cysteine-rich domain of EMILINs and other extracellular proteins, interacts with the gC1q domains and participates in ...
Plasticity and the extracellular matrix. Archived 2007-12-03 at the Wayback Machine Hensch TK (2005). "Critical period ...
... extracellular Extracellular matrix; D. Furst, muscle; Joseph Bonventre, kidney and related subjects; P. Sutovsky, reproductive ...
Dietz, J (2007). "Arterial stiffness and extracellular matrix". Atherosclerosis, Large Arteries and Cardiovascular Risk. Adv. ...
... extra-cellular polymers, nectar, root exudates and leachates, dissolved organic matter, extra-cellular matrix, mucilage). The ...
In particular, abnormal interactions between epithelial cells and the extracellular matrix are associated with the over- ...
extracellular matrix structural constituent. •protein binding. •extracellular matrix constituent conferring elasticity. • ... proteinaceous extracellular matrix. •mitochondrion. Processo biológico. •blood vessel remodeling. •skeletal muscle tissue ... extracellular matrix organization. •regulation of actin filament polymerization. •stress fiber assembly. Sources: Amigo / ...
collagen-containing extracellular matrix. العمليات الحيوية. • bone mineralization. • biomineral tissue development. • negative ...
... are often resistant to commonly used antifungal agents because of difficulty in penetrating the extracellular polymeric matrix. ...
extracellular matrix Reticular fibers. Form a scaffolding for other cells. Type III collagen. liver, bone marrow, and lymphatic ... Both the ground substance and proteins (fibers) create the matrix for CT. Connective tissues are derived from the mesenchyme. ... Cells are spread through an extracellular fluid.. *Ground substance - A clear, colorless, and viscous fluid containing ...
They are a type of extracellular matrix (ECM) protein, which, together with ameloblastins, enamelins, and tuftelins direct the ... mineralization of enamel to form a highly organized matrix of rods, interrod crystal, and protein. Although the precise role of ...
MPP+ eventually travels to the extracellular fluid by a dopamine transporter, which ultimately causes the Parkinson's symptoms ...
... s can be derived from the endoplasmic reticulum and replicate by fission.[13] Peroxisome matrix proteins are ... of peroxisomal matrix proteins signals them to be imported into the organelle. There are at least 32 known peroxisomal proteins ... is translocated into the peroxisomal matrix and recycled to the cytosol". Cell. 105 (2): 187-96. doi:10.1016/s0092-8674(01) ... and may occur without the import of the matrix (lumen) enzymes. Proliferation of the organelle is regulated by Pex11p. ...
One of the parts of the extracellular matrix is a complex molecule called a proteoglycan. Like many components of the body, ... The matrix surrounds the cells of the body in an organized meshwork and functions as the glue that holds the cells of the body ... This matrix is made up of a variety of sugars and proteins and helps to form the architectural framework of the body. ... of Hunter syndrome is related to a problem in a part of the connective tissue of the body known as the extracellular matrix. ...
"PTEN interactions with focal adhesion kinase and suppression of the extracellular matrix-dependent phosphatidylinositol 3- ... extracellular region. • extracellular. • synaptic vesicle. • axon. • dendrite. Biological process. • brain-derived neurotrophic ...
Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis For Repair and Tissue Engineering, M. Goldberg and A.J ... Dentin is a bone-like matrix that is porous and yellow-hued material. It is made up of 70% inorganic materials (mainly ... The innermost layer of dentin is known as predentin, and is the initial dentin matrix that is laid down prior to mineralisation ... Matrix metalloproteinases (MMPs) and their specific tissue inhibitors (TIMPs) in mature human odontoblasts and pulp tissue. ...
... sequences so the transplantation cells have closely related properties to that of native tissue in the extracellular matrix.[3] ... the inability of a sufficient matrix to thrive and create a uniform population of cells, or the migratory response of the cells ... by the body and biological functions such as cell adhesion and growth will be enhanced through cell-cell and cell-matrix ...
Beginning with a small 2 x 2 matrix, participants copy the matrix pattern from memory into an empty matrix. The matrix patterns ... Hebert, A. E.; Dash, P. K. (2002). "Extracellular signal-regulated kinase activity in the entorhinal cortex is necessary for ... The matrix patterns are arranged in a way that is difficult to code verbally, forcing the participant to rely on visual spatial ... At the end, the participant is asked to indicate on a real matrix where the little man that he or she visualized finished. The ...
extracellular matrix. • cytosol. • cell nucleus. • neuron projection. • neuronal cell body. Biological process. • muscle organ ... extracellular exosome. • stress fiber. • plasma membrane. • Z disc. • cytoplasm. • cell cortex. • focal adhesion. • ...
proteinaceous extracellular matrix. • extracellular region. • extracellular exosome. • extracellular space. • extracellular ... extracellular matrix constituent conferring elasticity. • identical protein binding. • integrin binding involved in cell-matrix ... It is the best characterized member of the EMILIN family of extracellular matrix glycoproteins. ... matrix. • integrin alpha4-beta1 complex. • EMILIN complex. Biological process. • cell adhesion. • cell-matrix adhesion. • cell ...
Extracellular matrix *Cell wall. Retrieved from "" ...
extracellular exosome. • نواة. • منطقة خارج الخلية. • collagen-containing extracellular matrix. العمليات الحيوية. • ...
... accelerating extracellular matrix and collagen formation and thus reducing the time for the healing process to occur.[22] ... "The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor". Biomaterials. 35 ...
The majority of mammalian ova are covered in a layer of granulosa cells intertwined in an extracellular matrix that contains a ... By catalyzing the hydrolysis of hyaluronan, a constituent of the extracellular matrix (ECM), hyaluronidase lowers the viscosity ... "Matrix Biology. 20 (8): 499-508. doi:10.1016/S0945-053X(01)00172-X. PMID 11731267.. ... although exogenous hyaluronidases can disrupt the cumulus matrix. ...
extracellular matrix organization. • positive regulation of sequence-specific DNA binding transcription factor activity. • ... extracellular region. • plasma membrane. • membrane raft. • extracellular space. Biological process. • regulation of protein ...
Extracellular matrix. Retrieved from "" ...
Ingelesez) «Extracellular matrix structure» Advanced Drug Delivery Reviews (97): 4-27 2016-02-01 doi:10.1016/j.addr.2015.11.001 ... Ingelesez) Mecham, Robert (2011-02-16) The Extracellular Matrix: an Overview Springer Science & Business Media ISBN ... Yue, Beatrice (2014) «Biology of the Extracellular Matrix: An Overview» Journal of glaucoma: S20-S23 doi:10.1097/IJG. ... The Extracellular Matrix of Animals» Molecular Biology of the Cell. 4th edition . Noiz kontsultatua: 2018-11-22 . ...
Additionally, the extracellular matrix and dense outer layer of bacterial cells can protect the inner bacteria cells from ... biofilms by adhering to surfaces on implanted devices such as catheters and prostheses and creating an extracellular matrix for ...
... matrix 1 protein), M2, NS1 (non-structural protein 1), NS2 (other name is NEP, nuclear export protein), PA, PB1 (polymerase ... "A universal influenza A vaccine based on the extracellular domain of the M2 protein". Nat. Med. 5 (10): 1157-63. doi:10.1038/ ...
extracellular region. • platelet alpha granule lumen. • extracellular. Biological process. • regulation of gene expression by ... and Matrix Attachment Region −3 (MAR3). These three DNA sequences bind to CTCF in a way that limits downstream enhancer access ...
... extracellular matrix attachment. The N-linked glycosylation process occurs in eukaryotes in the lumen of the endoplasmic ...
... is a medical condition characterized by widespread swelling of the skin due to effusion of fluid into the extracellular space; ... diagonal matrix, ph. [math.] వికర్ణ మాత్రిక;. *diagonal, n. వికర్ణం; కర్ణం; ఐమూలరేఖ;. *diagonalization, n. వికర్ణీకరణం; ఒక ...
Extracellular matrix. Illustration depicting extracellular matrix (basement membrane and interstitial matrix) in relation to ... The animal extracellular matrix includes the interstitial matrix and the basement membrane.[5] Interstitial matrix is present ... Extracellular matrix: review of its roles in acute and chronic wounds. *Usage of Extracellular Matrix from pigs to regrow human ... In biology, the extracellular matrix (ECM) is a three-dimensional network of extracellular macromolecules, such as collagen, ...
Media in category "Extracellular matrix proteins". The following 85 files are in this category, out of 85 total. ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s007.ogv 15 s, ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s008.ogv 15 s, ... Glioma-Initiating-Cells-Form-a-Differentiation-Niche-Via-the-Induction-of-Extracellular-Matrices-pone.0059558.s009.ogv 15 s, ...
Matrigel Matrix , Extracellular Matrix , Corning. We use cookies to ensure the best experience on our website. ... Cells behave better on Corning Matrigel matrix-the original, trusted extracellular matrix (ECM).. Nearly 30 years ago, ... Corning Matrigel Matrix FAQs Corning Matrigel Matrix FAQs Get answers to commonly asked questions about Corning Matrigel matrix ... Extracellular matrices are complex biological reagents, and, like all biologically derived reagents, they may be subject to lot ...
Used to study apoptosis, cell signaling pathways and gene expression. MP Biomedicals Hormones: Dexamethasone acts as anti-inflammatory glucocorticoid that induces the production of phospholipase A2 inhibitory protein (lipocortin). ...
... by the extracellular matrix. The proteins and glycoconjugates that make up the extracellular matrix provide structural support ... Biology of Extracellular Matrix. Copyright. 2013. Publisher. Springer-Verlag Berlin Heidelberg. Copyright Holder. Springer- ... The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement ... Biology of Extracellular Matrix Free Preview © 2013. Evolution of Extracellular Matrix. Editors: Keeley, Fred W., Mecham, ...
The extracellular matrix (ECM) is an acellular three-dimensional network composed of proteins, glycoproteins, proteoglycans and ... "Extracellular Composite Matrices in Arthropods" published by Springer in 2016.. Hans Merzendorfer is appointed as a Professor ...
Stress relaxation properties of the matrix as well as water transport through aquaporin-1 enable extracellular vesicles to ... which requires EVs to traverse the extracellular matrix (ECM). However, given that the size of EVs is usually larger than the ... Matrix stress relaxation allows EVs to overcome the confinement, and a higher crosslinking density facilitates a fluctuating ... Using engineered hydrogels, we demonstrate that the mechanical properties of the matrix regulate anomalous EV transport under ...
Many of the interactions have also been demonstrated on tissue sections or in vivo, and adherence to the extracellular matrix ... This review summarizes our current knowledge on the mechanisms of bacterial adherence to extracellular matrices and on the ... Some of these bacterial proteins are highly specific for an extracellular matrix protein, some are multifunctional and express ... Pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e ...
The extracellular matrix of the human optic nerve.. Goldbaum MH1, Jeng SY, Logemann R, Weinreb RN. ... The nerve fibers of the optic nerve are enclosed and segmented by extracellular matrix. With immunostains, we localized ... and fibronectin in frozen sections of the extracellular matrix of the prelaminar, laminar, and retrolaminar human optic nerve. ... The internal limiting lamina of the optic nerve has an extracellular composition similar to the thicker adjacent retinal ...
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the ... The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the ... Life Science > Stem Cell Biology > 3-D Stem Cell Culture > Learning Center > Extracellular Matrix ... The extracellular matrix and blood vessel formation: not just a scaffold, J. Cell. Mol. Med. 11(2): 176-205 (2007). ...
... Michael G. Hahn hahn at MOND1.CCRC.UGA.EDU Tue Nov 14 15:26:40 EST 1995 * ... Matrix Polysaccharides, Callose, and Cellulose Deborah Delmer, Bruce Wasserman, John Ralph Role of the Extracellular Matrix in ... roles of the extracellular matrix in plant development and in interaction= s with sybiotic and pathogenic organisms, and the ... The Extracellular Matrix of Plants: Molecular, Cellular and Developmental Biology Tamarron, Colorado. March 15-21, 1996 The ...
Here we demonstrate the ability of decellularized liver extracellular matrix (dECM) hydrogels to induce the in vitro formation ... Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels. Tissue Eng. Part A 16, 1075-1082 ... Complex bile duct network formation within liver decellularized extracellular matrix hydrogels. *Phillip L. Lewis1,2. , ... The extracellular matrix (ECM) of the developing liver plays an integral role in the formation and maturation of the biliary ...
Kliniken & Institute … Kliniken Zentrum für Innere… Innere Medizin III:… Forschung Grundlagenforschung … Extracellular Matrix… ... AG Extracellular Matrix and Integrins in Cardiovascular Disease. Integrin signaling and heart failure Integrins are ... By establishing a Tamoxifen (Tx) inducible knockout mouse, we have demonstrated that the extracellular matrix glycoprotein ... In this project we analyze effects of extracellular cues for hypertrophic remodeling and examine transcription factor circuits ...
... Nat Mater. 2012 May 27;11(7):642-9. doi: 10.1038/nmat3339. ... could not form stable focal adhesions and differentiated as a result of decreased activation of the extracellular-signal- ...
... is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and blood vessels. ... a new extracellular matrix glycoprotein localized on collagen fibrils. Matrix Biol 15:11-19PubMedCrossRefGoogle Scholar ... Tenascin-X (TNX) is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and ... Bristow J, Tee MK, Gitelman SE, Mellon SH, Miller WL (1993) Tenascin-X: a novel extracellular matrix protein encoded by the ...
1988) Extracellular matrix specificity for the differentiation of capillary endothelial cells. Exp. Cell Res., 178: 426-434. ... 1991) Effects of soluble factors and extracellular matrix components on vascular cell behavior in vitro and in vivo: models of ... Madri, J.A., Pratt, B.M., and Yannariello-Brown, J.(1989) Endothelial cell-extracellular matrix interactions. In: Endothelial ... 1978) An ultrastructural study of developing extracellular matrix in vitelline blood vessels of the early chick embryo. Am. J. ...
In biology, the extracellular matrix (ECM) is the extracellular part of multicellular structure (e.g., organisms, tissues, and ... to pro-anabolic upon integration to the extracellular matrix," Matrix Biology, vol. 31, no. 5, pp. 290-298, 2012. View at ... P. G, "The extracellular matrix and cell adhesion," in Cells, B. Lewin, L. Cassimeris, V. Lingappa, and G. Plopper, Eds., 2007. ... "Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia," Matrix ...
Extracellular matrix composition significantly influences pancreatic stellate cell gene expression pattern: role of transgelin ...
1993) Extracellular matrix 3: Evolution of the extracellular matrix in invertebrates. FASEB J 7:1115-1123.. ... Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro. Yael Helman, Frank Natale, Robert ... 2000) Extracellular matrix (ECM) components in a very primitive multicellular animal, the dicyemid mesozoan Kantharella ... Extracellular production of organic matrices and calcium carbonate particles was examined in primary, nondividing cell cultures ...
Bonnans C, Chou J, Werb Z. Remodelling the extracellular matrix in development and disease. Nat Rev Mol Cell Biol. 2014;15(12): ... Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques. Sarah R. Langley,1,2 Karin ... The subendothelial extracellular matrix modulates JNK activation by flow. Circ Res. 2009;104(8):995-1003.. View this article ... Extracellular matrix composition and remodeling in human abdominal aortic aneurysms: a proteomics approach. Mol Cell Proteomics ...
There is described herein methods of treating a disease associated with extracellular matrix (ECM) in a patient. In some cases ... 1 shows extracellular matrix accumulation (ECM) in normal and fibrotic skin corresponds to a shift in metabolism from fatty ... 21 shows extracellular matrix deposition in the murine skin fibrosis after CD36high fibroblast vs. vehicle treatment. Tissue ... The skin is the largest organ of the human body and 70% of its dry weight is comprised of extracellular matrix (ECM). The ...
Immobilization after injury alters extracellular matrix and stem cell fate. Amanda K. Huber,1 Nicole Patel,1 Chase A. Pagani,1 ... Cells sense extracellular environment and mechanical stimuli and translate these signals into intracellular responses through ...
A protective extracellular matrix underpins HIV infectivity. All press releases are available in the Press area of the Institut ... A protective extracellular matrix underpins HIV infectivity. A "viral biofilm" (in green) produced at the surface of a CD4 T ... HomePress areaPress documentsA protective extracellular matrix underpins HIV infectivity ... transported in an adhesive extracellular matrix, which also shelters them from the immune system and antiretroviral drugs. "We ...
... Curr Opin Cell Biol. 1996 Oct;8(5):625-31. doi: ...
The impact of decellularization agents on renal tissue extracellular matrix Nafiseh Poornejad, Lara B. Schaumann, Travis ... leaving intact collagenous extracellular matrices (ECMs). Although complete removal of cellular remnants should be achieved to ...
The purpose of this review is to summarize the established and proposed changes to the hepatic extracellular matrix (ECM) that ... The fibrotic stage of ALD is primarily characterized by robust accumulation of extracellular matrix (ECM) proteins (collagens) ... "The extracellular matrix: tools and insights for the omics era," Matrix Biology, vol. 49, pp. 10-24, 2016. View at: Publisher ... The extracellular matrix (ECM) consists of a diverse range of components that work bidirectionally with surrounding cells to ...
... extracellular matrix organization, positive regulation of cell-substrate adhesion ... Extracellular region or secreted. *extracellular matrix Source: GO_CentralInferred from biological aspect of ancestori*. " ... extracellular matrix organization Source: GO_CentralInferred from biological aspect of ancestori*. "Phylogenetic-based ... Extracellular matrix protein 2Imported. ,p>Information which has been imported from another database using automatic procedures ...
... and postsynaptic neuronal elements with astroglial processes and extracellular matrix (ECM) for formation and plasticity of ... Here we outline the major mechanisms driving: (i) synaptogenesis by secreted extracellular scaffolding molecules, like ... Here we outline the major mechanisms driving (i) synaptogenesis by secreted extracellular scaffolding molecules, like ... Particularly, we focused on potential importance of activity-dependent concerted activation of multiple extracellular proteases ...
Hironobu Fujiwara on Cutaneous extracellular matrix, part of a collection of online lectures. ... Cutaneous extracellular matrix. *Dr. Hironobu Fujiwara - RIKEN Center for Developmental Biology (CDB) Kobe, Japan ... Fujiwara, H. (2014, May 4). Cutaneous extracellular matrix [Video file]. In The Biomedical & Life Sciences Collection, Henry ... Today Id like to talk about several of the extracellular matrices, ECM in mammalian skin. ...
  • Glycosaminoglycans (GAGs) are carbohydrate polymers and mostly attached to extracellular matrix proteins to form proteoglycans (hyaluronic acid is a notable exception, see below). (
  • In the extracellular matrix, especially basement membranes , the multi-domain proteins perlecan , agrin , and collagen XVIII are the main proteins to which heparan sulfate is attached. (
  • The development of Corning Matrigel matrix, a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors. (
  • The proteins and glycoconjugates that make up the extracellular matrix provide structural support to cellular complexes, facilitate cell adhesion and migration, and impart mechanical properties that are important for tissue function. (
  • The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement membranes. (
  • Bacterial proteins binding to the mammalian extracellular matrix. (
  • Pathogenic bacteria frequently express surface proteins with affinity for components of the mammalian extracellular matrix, i.e. collagens, laminin, fibronectin or proteoglycans. (
  • Some of these bacterial proteins are highly specific for an extracellular matrix protein, some are multifunctional and express binding activities towards a number of target proteins. (
  • The scheduled sessions include those on structural studies of the major macromolecules that make up the plant extracellular matrix (polysaccharides, proteins, lignin), biosynthesis of wall macromolecules,= roles of the extracellular matrix in plant development and in interaction= s with sybiotic and pathogenic organisms, and the importance of plant cell walls= in food and industry. (
  • Cartilage extracellular matrix (ECM) is composed primarily of the network type II collagen (COLII) and an interlocking mesh of fibrous proteins and proteoglycans (PGs), hyaluronic acid (HA), and chondroitin sulfate (CS). (
  • This protective mesh is composed principally of proteins and carbohydrates and is comparable to that of bacterial biofilm, an extracellular matrix network secreted by some bacteria as protection against their environment. (
  • 8,200 antibodies specific for proteins of the extracellular matrix. (
  • Background: The ADAM (A Disintegrin and A Metalloprotease) family of multidomain membrane proteins influences cell signaling and adhesion by shedding cell surface proteins such as cytokines and growth factors, by influencing cell adhesion to the extracellular matrix (ECM), and by directly remodeling the ECM. (
  • Phosphorylation of the cytoplasmic tail as well as its interaction with other signaling proteins may influence intra- and extracellular signaling (1). (
  • The glue is fibronectin, a cell adhesion protein that allows cells to attach to the different matrix elements, including collagen, GAGs, and integrins, membrane proteins that connect cells to the environment and play an important role in signaling cascades. (
  • AlphaD1 exits only of Alpha-helices, whereas SASDL2 and -3 are capable of binding most of the extracellular matrix proteins identified so far (collagen type IV, laminin 332, fibronectin , perlecan, fibulin 1C/D , fibulin-3 and MMP-9 ) (Sercu et al. (
  • Macromolecular crowding in extracellular culture media directly induced supramolecular assembly and alignment of extracellular matrix proteins deposited by cells, which in turn increased alignment of the intracellular actin cytoskeleton. (
  • This new edition of Guidebook to the Extracellular Matrix and Adhesion Proteins brings up-to-date information on the purification, activities, antibodies and genes for the proteins found in the extracellular matrix and those known to be involved in cell-cell contact and adhesion. (
  • Drawing on a wide range of sources, this volume addresses all aspects of the structure and function of modules in the extracellular matrix, adhesion molecules, cell adhesion and cell-cellcontact proteins. (
  • ECM2 encodes extracellular matrix protein 2, so named because it shares extensive similarity with known extracellular matrix proteins. (
  • CD36 is a busy molecule, regularly making contact with a wide variety of proteins in the extracellular matrix and on neighboring cells. (
  • The carboxy terminus of dystrophin binds to a plasma membrane anchor, [beta]-dystroglycan, which is associated on the external side with the extracellular matrix receptor, [alpha]-dystroglycan, which binds to the basal lamina proteins laminin 1, laminin 2, and agrin. (
  • Thus interaction between integrins and specific matrix proteins is responsible for sensing mechanical strain in vascular smooth muscle cells. (
  • to investigate the lymphocyte pathways regulating the viral biofilms formation and composition in extracellular matrix (ECM) proteins (using quantitative polymerase chain reaction (qPCR) and siRNA). (
  • In this study we explored the effect of selective extracellular matrix (ECM) proteins on the potential of insulin-producing cell differen-tiation using ARIP cells, an adult rat pancreatic ductal epithelial cell line, as a model in vitro. (
  • For example, matrix proteins sequester water that provides turgor to soft tissues and minerals that give rigidity to skeletal tissues. (
  • neurofascin-186 [NF-186] and neuron glia-related CAM [NrCAM]), cytoskeletal proteins (ankyrinG and βIV spectrin), and the extracellular chondroitin-sulfate proteoglycan brevican. (
  • Thus, the clustering of ion channels at nodes of Ranvier is thought to depend on binding to cytoskeletal and scaffolding proteins that are positioned along axons by extracellular, heterophilic interactions between axonal and glial CAMs ( Schafer and Rasband, 2006 ). (
  • Rather than linking two cells, hemi-desmosomes attach cells to the extracellular matrix and use integrin cell adhesion proteins rather than cadherins. (
  • We have shown that, in vivo , SCLC cells are surrounded by an extensive stroma of extracellular matrix (ECM) at both primary and metastatic sites which contains, among other proteins, fibronectin, laminin and collagen IV. (
  • Next, cell migration uses adhesion proteins on both the cell surface and in the extracellular matrix (ECM) to guide remodeling and cell movement through the environment. (
  • Tissue inhibitors of metalloproteinases (TIMPs) regulate the ECM turnover through negative regulation of matrix metalloproteinases (MMPs), which degrade the ECM structural proteins. (
  • 3 - 7 Matrix metalloproteinases (MMPs) degrade ECM proteins, whereas their proteolytic activity is kept in check by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). (
  • Proteolytic assays were performed with extracellular matrix proteins to assess the putative role of Cwp84 in the pathogenicity of C. difficile . (
  • P47 shows strong and specific binding to gastrointestinal tissues and some extracellular matrix proteins (type I collagen, thrombospondin, and vitronectin) ( 4 ). (
  • In fact, collagen is the most abundant protein in the human body [15] [16] and accounts for 90% of bone matrix protein content. (
  • Higher protein concentration provides greater matrix stiffness and scaffold integrity. (
  • The other protein components of the BM are thought to be non-covalently immobilized in the matrix formed by laminin and collagen IV6. (
  • However, cells on polyacrylamide of low elastic modulus (0.5 kPa) could not form stable focal adhesions and differentiated as a result of decreased activation of the extracellular-signal-related kinase (ERK)/mitogen-activated protein kinase (MAPK) signalling pathway. (
  • Tenascin-X (TNX) is a large 450 kDa extracellular matrix protein expressed in a variety of tissues including skin, joints and blood vessels. (
  • Bristow J, Tee MK, Gitelman SE, Mellon SH, Miller WL (1993) Tenascin-X: a novel extracellular matrix protein encoded by the human XB gene overlapping P450c21B. (
  • Synthetic peptide within Human Extracellular matrix protein 1 aa 100-200. (
  • In this regard, especially the matrix protein Tenascin C (Tnc) proved to be an important regulator of astrocyte precursor cell proliferation and migration during spinal cord development. (
  • We quantified the impact of induced crowding on the extracellular and intracellular protein organization of human mesenchymal stem cells (MSCs) via immunocytochemistry, atomic force microscopy (AFM), and AFM-enabled nanoindentation. (
  • Cysteine rich 61 (CCN1) is an extracellular matrix (ECM) protein elevated in cancer cells that modulates their adhesion and migration by binding cell surface receptors. (
  • It is a secreted protein which typically localizes in the extracellular matrix (ECM) and on the cell surface ( 5 ), in which it binds integrin receptors to modulate a variety of cellular functions including adhesion, migration, and proliferation ( 6 ). (
  • Extracellular matrix protein 2 is a protein that in humans is encoded by the ECM2 gene. (
  • Cells adhered, spread, and proliferated on each matrix protein, but the mitogenic response to strain was matrix dependent. (
  • Here, the use of the C-C motif chemokine ligand 25 (CCL)25 in comparison to differentiation factors such as transforming growth factor (TGF)β3, bone morphogenetic protein (BMP)2, BMP7, BMP12, and BMP14 (all in concentrations of 10, 50 and 100 ng/mL) was tested in an in vitro micro mass pellet model with isolated and cultivated human AF-cells ( n = 3) to induce and enhance AF-matrix formation. (
  • This work demonstrates a role for a brain-specific extracellular matrix protein in glioma invasion, opening new therapeutic avenues for a uniformly fatal disease. (
  • We now show that JNK activation by both onset of laminar flow and long-term oscillatory flow is matrix-specific, with enhanced activation on fibronectin compared to basement membrane protein or collagen. (
  • The roles of the protein-serine/threonine kinase, Akt1, in signaling pathways associated with cell motility and extracellular matrix invasion were examined in the immortalized mouse mammary epithelial cell line, COMMA-1D. (
  • The composition of the matrix of the interstitium and the proportion and the expression of each protein can have a profound influence on cardiac structure and compliance that will determine its hemodynamic functions ( 20 ). (
  • In biology , the extracellular matrix ( ECM ) is a three-dimensional network of extracellular macromolecules , such as collagen , enzymes , and glycoproteins , that provide structural and biochemical support of surrounding cells. (
  • [5] Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). (
  • [7] Some single-celled organisms adopt multicellular biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS). (
  • Cells behave better on Corning Matrigel matrix-the original, trusted extracellular matrix (ECM). (
  • The evolution of single cells into multicellular organisms was mediated, in large part, by the extracellular matrix. (
  • Cells release extracellular vesicles (EVs) to communicate over long distances, which requires EVs to traverse the extracellular matrix (ECM). (
  • The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. (
  • Here we demonstrate the ability of decellularized liver extracellular matrix (dECM) hydrogels to induce the in vitro formation of complex biliary networks using encapsulated immortalized mouse small biliary epithelial cells (cholangiocytes). (
  • Cells attach to proteoglycans and glycoproteins on the surface of other cells as well as in the extracellular matrix (ECM) substratum via adhesion molecules to define tissue shape, structure, and function. (
  • In biology, the extracellular matrix (ECM) is the extracellular part of multicellular structure (e.g., organisms, tissues, and biofilms) that typically provides structural and biochemical support to the surrounding cells [ 1 ]. (
  • Integrins are transmembrane receptors that mediate the attachment between a cell and its surroundings, such as other cells or the extracellular matrix (ECM). (
  • The evolution of multicellularity in animals required the production of extracellular matrices that serve to spatially organize cells according to function. (
  • In this report, we examine the production of these three matrices by using an in vitro culturing system for coral cells. (
  • Using HIV-infected CD4+ T cells, Dr Thoulouze and colleagues analyzed the extracellular mesh formed at the surface of these cells and evaluated viral infection after its destruction. (
  • Effective decellularization uses agents that lyse cells and remove all cellular materials, leaving intact collagenous extracellular matrices (ECMs). (
  • The agreement is based upon Kensey Nash 's proprietary tissue processing technology, known as the Optrix process, which gently disinfects tissues, inactivates viruses and removes cells while preserving extracellular matrix components. (
  • Human prolactin secreting adenoma cells maintained on extracellular matrix. (
  • In vitro maintenance of human prolactin secreting adenoma cells on a extracellular matrix (ECM) produced by bovine corneal endothelial cells is associated with the adoption of morphological properties that are not expressed when the cells are seeded on plastic. (
  • Both tumor cells and normal cells are more likely to resemble their in vivo counterparts when maintained on extracellular matrix than on plastic, and therefore, cells cultured on ECM may provide a better system for oncogenic and endocrine studies. (
  • In biology, the extracellular matrix ( ECM ) is the extracellular part of animal tissue that usually provides structural support to the animal cells in addition to performing various other important functions. (
  • Methods for culturing eukaryotic cells and studying their growth characteristics, including the invasive growth characteristics of tumor cells, on the submucosal matrix are described. (
  • Supporting many cellular tissues is the extracellular matrix, or ECM, an interconnected network composed of fibers and ground substance, which is mostly interstitial fluid that fills the space between the cells, connective tissue fibers, and capillaries. (
  • In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). (
  • In biology, the extracellular matrix (ECM) is a three-dimensional network consisting of extracellular macromolecules and minerals, such as collagen, enzymes, glycoproteins and hydroxyapatite that provide structural and biochemical support to surrounding cells. (
  • This is realized using an etched silicon pillar flow chamber filled with extracellular matrix (ECM) gel mixed with cells. (
  • Coloured scanning electron micrograph (SEM) of the extracellular matrix (ECM) found around multicellular aggregates of yeast cells, such as biofilms. (
  • These reactive species ultimately eliminate highly proliferating cells by damaging membranes, DNA and other cell structures, and also by affecting extracellular matrix (ECM) components. (
  • Are factors originating from serum, plasma, or cultured cells involved in the growth-promoting effect of the extracellular matrix produced by cultured bovine corneal endothelial cells? (
  • The molecules of the extracellular matrix (ECM) comprise the connective tissues of animals and form basal laminae underlying sheets of epithelial cells. (
  • Our work elucidated a highly intriguing question: how cells move when they are in the complex and physiologically relevant environment of a 3-D extracellular matrix ," said Hyun (Michel) Koo, a professor in the Department of Orthodontics at Penn's School of Dental Medicine. (
  • Mechanical strain of rat vascular smooth muscle cells is sensed by specific extracellular matrix/integrin interactions. (
  • It is not clear whether the extracellular matrix plays only a passive role or a more active, instructive role in shaping organs, in part, because it is difficult to measure the physical forces within densely packed cells. (
  • This work reveals that, along with providing structural support to cells, the mechanical properties of the matrix also actively guide how organs form. (
  • resistance to these forces is mediated by cells and by the extracellular matrix (ECM), including the basement membranes (BMs) that line all epithelia. (
  • Here, though, and increasingly in scientific and research circles and professionals worldwide, 'fascia' has a wider definition: all the collagenous-based soft-tissues in the body, including the cells that create and maintain that network of extra-cellular matrix (ECM). (
  • The composition of the extracellular matrix may be a critical factor in determining the invasive potential of cancer cells, such that the production of matrix elements by glioma cells might mediate their invasion into normal tissue. (
  • Behaviors characteristic of cells in the developing brain, such as cell proliferation and migration, neuronal and glial process outgrowth, and the elaboration of the capillary network, take place in a soluble matrix that is permissive for cell movement. (
  • A model of extracellular matrix (ECM) of collagen fibers has been built, in which cells could communicate with distant partners via fiber-mediated long-range-transmitted stress states. (
  • The Extracellular matrix (ECM) is the network of extracellular macromolecules that provide cells structural and biochemical support. (
  • The Extracellular Matrices and Substrates are a group of products that provide such support for 2D and 3D cell culture research and allow for more options to provide the most in vivo -like environment for the specific cells that are being used. (
  • The extra-cellualr matrix (ECM) is secreted locally and assembles into a network in the spaces surrounding cells (intercellualr space). (
  • The interstitial matrix is present in spaces between epithelial, endothelial, and smooth muscle cells and in connective tissue . (
  • The cells in tissue are in contact with secreted extracellular macromolecules referred to as the extracellular matrix . (
  • The matrix helps hold the cells and tissues together and provides a means in which cell can migrate and interact. (
  • In vivo, cells are exposed to mechanical forces as a consequence of blood flow, interstitial flow, blood pressure, and extracellular matrix (ECM) stiffness. (
  • Matrix metalloproteinase (MMP)-1, -3 and MMP-13 expression was reduced in aging ACL but increased in degenerated ACL, mainly in the chondrocyte-like cells. (
  • Tight junctions prevent the movement of the extracellular matrix between cells. (
  • The nonliving matrix of the connective tissue through which the blood cells freely flow is the? (
  • Extracellular matrix provides the microenvironment for the cells and serves as a tissue scaffold, guiding cell migration during embryonic development and wound repair. (
  • The features of the ECM are determined both by the cells that produce the matrix and by the cells growing in it. (
  • Cell adhesion is the binding of the cells to each other and to the extracellular matrix through cell adhesion molecules (CAMs) such as integrins, selectins, cadherins, the Ig (immunoglobulin) superfamily, and lymphocyte homing receptors. (
  • Thus, extracellular macromolecules influence and organize not only the basal cell surface but also the actin-rich basal cell cortex of epithelial cells. (
  • Cancer cell invasion and metastasis is a complex, multistep process involving interactions between invading cells, the extracellular matrix, and other stromal elements ( 1 ). (
  • Proteolytic enzymes secreted by tumor and/or host cells are required for cancer cells to invade the extracellular matrix and infiltrate lymphatic or blood vessel walls to metastasize to regional or distant sites. (
  • The collagen fibrils that these cells synthesise provide a template for postnatal growth by structure-based matrix expansion. (
  • Extracellular matrix (ECM) mechanical stiffness and its dynamic change is one of the main cues that directly affects the differentiation and proliferation of normal cells as well as the progression of disease processes such as fibrosis and cancer. (
  • The injured tissue environment upon sensing the stress turns on a precisely orchestrated network of immune responses by regulating cytokine-chemokine production, recruitment of immune cells, and modulating fibrogenic niche and extracellular matrix (ECM) cross-talk during fibrotic pathologies like cardiac fibrosis, liver fibrosis, laryngotracheal stenosis, systemic scleroderma, interstitial lung disease and inflammatory bowel disease. (
  • These macromolecules are present in intercellular junctions and cell surfaces and may assemble into two general organizations: interstitial matrix and basement membrane (BM). (
  • the myocardial extracellular matrix (ECM) represents a complex three-dimensional network of various macromolecules. (
  • This volume in the Biology of Extracellular Matrix series provides a review of the known classes of proteases that degrade ECM both outside and inside the cell. (
  • The topics covered in this volume provide an important context for understanding the role that matrix-degrading proteases play in normal tissue remodeling and in diseases such as cancer and lung disease. (
  • Comparative genomics has identified a conserved core ECM adhesome, that includes integrin adhesion receptors, matrix proteases and the central ECM components of connective ECM and basement membranes. (
  • Particularly, we focused on potential importance of activity-dependent concerted activation of multiple extracellular proteases, such as ADAMTS4/5/15, MMP9 and neurotrypsin, for permissive and instructive events in synaptic remodeling through localized degradation of perisynaptic ECM and generation of proteolytic fragments as inducers of synaptic plasticity. (
  • Collagen is exocytosed in precursor form (procollagen), which is then cleaved by procollagen proteases to allow extracellular assembly. (
  • Due to the potential applications of this therapy, many studies have been reported regarding the effect of the treatment on cell survival/death, cell proliferation, matrix assembly, proteases and inhibitors, among others. (
  • Among proteases, the matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family are often associated with ECM degradation and remodeling. (
  • The Human Extracellular Matrix & Adhesion Molecules RT² Profiler PCR Array profiles the expression of 84 genes important for cell-cell and cell-matrix interactions. (
  • The Mouse Extracellular Matrix & Adhesion Molecules RT² Profiler PCR Array profiles the expression of 84 genes important for cell-cell and cell-matrix interactions. (
  • The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules. (
  • However, the early astrocytes influence their environment not only by releasing and responding to diverse soluble factors but also express a wide range of extracellular matrix (ECM) molecules, in particular proteoglycans of the lectican family and tenascins. (
  • Thus, the focus of the present paper is related to the effects of PDT observed on the extracellular matrix and on the molecules associated with it, such as, adhesion molecules, matrix metalloproteinases, growth factors, and immunological mediators. (
  • Thus, cell migration comprises cell-cell adhesion and cell-ECM interaction and is orchestrated by cell adhesion molecules and integrin receptors, ECM components, chemoattractant molecules, matrix proteinases, and glycosidases. (
  • EFEMP1 is one member of fibulins that serve to modulate cellular behavior and functions by connecting and integrating multiple partner molecules in extracellular compartment. (
  • The Extracellular Matrix and Cell Adhesion PCR Arrays are designed to determine the gene expression profile of the molecules involved in cell-to-cell and cell-to-matrix interactions. (
  • Response of basal epithelial cell surface and Cytoskeleton to solubilized extracellular matrix molecules. (
  • Loss of flexibility is due at least in part to chemical changes in the Extracellular Matrix (ECM) in the tissue, converting a flexible network of molecules into a rigid mesh. (
  • Regulated turnover of extracellular matrix (ECM) is an important component of tissue homeostasis. (
  • Many of the interactions have also been demonstrated on tissue sections or in vivo, and adherence to the extracellular matrix has been shown to promote bacterial colonization of damaged tissues. (
  • They orchestrate cell-cell and cell-matrix adhesive interactions from embryonic development to mature tissue function. (
  • Cartilaginous ECM is remodeled continuously by a combination of production, degradation by matrix metalloproteinases (MMPs), and inhibition of MMPs activity by tissue inhibitors of MMPs [ 4 ]. (
  • The metazoan extracellular matrix (ECM), an extracellular system of insoluble networks, sheets and fibrils of proteinaceous material, is a central mediator of multicellularity that is required for developmental processes, tissue organisation, homoeostasis and control of cell phenotypes throughout life. (
  • The extracellular matrix is the defining feature of connective tissue in animals. (
  • The extracellular matrix is vital for tissue organization in multicellular organisms. (
  • To isolate extracellular matrix from the kidney, place the diced cortex tissue into the beaker containing SDS solution. (
  • This conference will focus on the converging roles of the extracellular matrix (ECM) and soluble factors for the repair or replacement of tissue lost or damaged due to degenerative disease, cancer, or trauma. (
  • Studies based on Efemp1 knock-out mouse implicate EFEMP1 function in withholding tissue integrity by stimulating the expression of Timp1 and Timp3 and inhibiting the expression and activities of matrix metalloproteinase Mmp2 , and Mmp9 (Rahn et al. (
  • The extra-cellualr matrix (ECM) forms a significant proportion of the volume of any tissue. (
  • Tissue and extracellular matrix (ECM) stiffness is transduced into intracellular stiffness, signaling, and changes in cellular behavior. (
  • Preparation and Characterization of a Chitosan/Gelatin/Extracellular Matrix Scaffold and Its Application in Tissue Engineering. (
  • Previous studies have demonstrated that extracellular matrix (ECM) can be used in tissue engineering due to its bioactivity. (
  • The ability of tendons to transmit such large forces is directly attributable to an extracellular matrix (ECM) comprising collagen fibrils aligned parallel to the tissue long axis. (
  • However, the absence of a robust method of imaging cell-matrix organisation has precluded a detailed study of how the cell-rich anlage found in embryogenesis grows into a mechanically strong connective tissue in the adult. (
  • Additionally, all tissue-based neutrophilic responses to fungal infections necessitate contact with the extracellular matrix (ECM). (
  • 56% women), we related circulating concentrations of matrix metalloproteinase-9 (binary variable: detectable versus undetectable), log of tissue inhibitor of matrix metalloproteinase-1, and log of procollagen type III aminoterminal peptide (PIIINP) to incident CVD and death. (
  • Each SD increment of log of tissue inhibitor of matrix metalloproteinase-1 and log-PIIINP was associated with multivariable-adjusted hazards ratios of 1.72 (95% CI, 1.30 to 2.27) and 1.47 (95% CI, 1.11 to 1.96), respectively, for mortality risk. (
  • Conclusion- Higher circulating tissue inhibitor of matrix metalloproteinase-1 and PIIINP concentrations are associated with mortality, and higher PIIINP is associated with incident CVD, in the community. (
  • The abstract deadline for the Keystone Meeting on The Extracellular Matri= x of Plants: Molecular, Cellular and Developmental Biology HAS BEEN EXTENDED to November 20, 1995. (
  • Articular cartilage ECM plays a crucial role in regulating chondrocyte metabolism and functions, such as organized cytoskeleton through integrin-mediated signaling via cell-matrix interaction. (
  • Thus, NF-186 assembles and links the specialized brevican-containing AIS extracellular matrix to the intracellular cytoskeleton. (
  • His work ties in with that of Donald Ingber on tensegrity within the cytoskeleton, and the links between the cytoskeleton and the Extra Cellular Matrix as described by James Oschman. (
  • Integrins are heterodimers of α- and β - subunits that bind to the ECM through large extracellular domains and connect intracellularly to the actin cytoskeleton filaments. (
  • I ) In the absence of extracellular signals, BMPRs and integrins do not engage without ligand binding. (
  • Subsequent binding of newly activated integrins to extracellular matrix (ECM) initiates downstream signals. (
  • Hyaluronic acid in the extracellular space confers upon tissues the ability to resist compression by providing a counteracting turgor (swelling) force by absorbing significant amounts of water. (
  • The expression of these gelatinases is relatively low in normal tissues and is induced when extracellular matrix remodeling is required. (
  • The interstitial ECM, which makes up most of the extracellular mass of the body, consists of many different types of collagen, tenascin, proteoglycans and elastin in elastic tissues such as skin or tendon. (
  • Recent advancements in biomaterials have enabled a wide range of polymer matrices that could mimic the ECM of different tissues for a wide range of in vitro basic research and drug discovery. (
  • However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. (
  • L. M. Coussens and Z. Werb, Matrix metalloproteinases and the development of cancer ,, Chemistry and Biology , 3 (1996), 895. (
  • Matrix metalloproteinases (MMPs) degrade ECM components and regulate normal cell migration as well as tumor cell invasion and metastases ( 5 , 6 ). (
  • Matrix metalloproteinases (MMP), especially MMP2, are commonly overexpressed in squamous cell carcinoma and are known to promote invasion through degradation of collagen in the basement membrane. (
  • Several studies suggest that matrix metalloproteinases (MMP), a family of zinc-dependent endopeptidases, play a significant role in extracellular matrix invasion ( 3 ). (
  • The podosome rosettes set the scene for global extracellular matrix degradation and internalization. (
  • The extracellular matrix (ECM) is a complex network of different combinations of collagens, proteoglycans, hyaluronic acid, laminin, fibronectin, and many other glycoproteins, including proteolytic enzymes involved in degradation and remodeling of the extracellular matrix. (
  • The attachment and growth of bacteria on these membranes results in formation of biofilms: to create a favourable living environment they immobilize themselves in a slimy matrix of extracellular polymeric substances (EPS). (
  • Down: Microscopic image of a biofilm, showing bacteria and extracellular polymeric substances. (
  • With immunostains, we localized collagen types I through VI, laminin, and fibronectin in frozen sections of the extracellular matrix of the prelaminar, laminar, and retrolaminar human optic nerve. (
  • When strain was applied on matrices containing both laminin and vitronectin, the mitogenic response to strain depended upon the vitronectin content of the matrix. (
  • The extracellular ligands that anchor these adhesions include laminin, fibronectin, vitronectin, and various collagens. (
  • By establishing a Tamoxifen (Tx) inducible knockout mouse, we have demonstrated that the extracellular matrix glycoprotein fibronectin (Fn), activates critical survival signals via ß1 integrin following myocardial infarction (1). (
  • Finally, we determined the functional importance of integrin-fibronectin interactions and matrix metalloprotease (MMP)-facilitated cell movement during re-epithelialization and propose a model for cell locomotion during postmenstrual repair. (
  • Described below are the different types of proteoglycan found within the extracellular matrix. (
  • A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. (
  • In addition to ECM-intracellular signal transduction, cell-cell communication takes place in the extracellular environment when a chemical secreted by a signaling cell interacts with a receptor on the membrane of a second (signal receiving) cell (2). (
  • Therefore, tight extracellular and intracellular regulation of BMP signaling is critical. (
  • 3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D ANNOUNCEMENT (Reminder) Keystone Symposium: The Extracellular Matrix of Plants: Molecular, Cellular and Developmental Biology Tamarron, Colorado. (
  • Cellular and extracellular matrix changes in anterior cruciate ligaments during human knee aging and osteoarthritis. (
  • Hypoxia promotes extracellular matrix (ECM) remodeling, cellular metabolic adaptation, and cancer cell metastasis. (
  • QIAGEN provides a broad range of assay technologies for extracellular matrix and adhesion molecule research that enable analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. (
  • The Gordon Research Conference (GRC) on Signal Transduction by Engineered Extracellular Matrices was established in 2000 to enhance the intersection of bioengineering and cell biology with an aim to foster translation of cell-based technologies into practice. (
  • This GRC will be held in conjunction with the "Signal Transduction by Engineered Extracellular Matrices (GRS)" Gordon Research Seminar (GRS). (
  • The internal limiting lamina of the optic nerve has an extracellular composition similar to the thicker adjacent retinal internal limiting lamina. (
  • The invention relates to extracellular matrix (ECM) accumulation and more particularly to composition and methods for either increasing or decreasing the same. (
  • Here we present a protocol to fabricate a kidney cortex extracellular matrix-derived hydrogel to retain the native kidney extracellular matrix (ECM) structural and biochemical composition. (
  • For a basic understanding of the chemical and physical interactions of the EPS matrix, model biofilms with increasing complexity are designed and the influence of structure and composition on the physical properties is investigated. (
  • Bosman FT, Stamenkovic I. Functional structure and composition of the extracellular matrix. (
  • Native extracellular matrix preserves mesenchymal stem cell 'stemness' and differentiation potential under serum-free culture conditions. (
  • The investigators' preliminary data indicate that besides ' free ' infectious viral particles, HIV-1 infected cluster of differentiation 4 (CD4+) lymphocytes also produce extracellular viral assemblies wrapped in an extracellular matrix cocoon and tightly bound to the surface of the cell. (
  • EFEMP1 is likely to contribute the integrity of basement membrane zones and anchor other extracellular matrix structures such as elastic fibers to basement membranes. (
  • The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology. (
  • In particular, this meeting will focus on the role that engineered matrices can play in advancing developmental biology and in vitroorganogenesis, single cell biology, microphysiological models and biofabrication ( i.e. "organs-on-chips"), regenerative medicine therapies, and immunology to study human health and disease. (
  • Jemma Evans , Tu'uhevaha Kaitu'u-Lino , and Lois A. Salamonsen "Extracellular Matrix Dynamics in Scar-Free Endometrial Repair: Perspectives from Mouse In Vivo and Human In Vitro Studies," Biology of Reproduction 85(3), 511-523, (25 May 2011). (
  • The basement membrane (BM) and extracellular matrix (ECM) play critical roles in developmental and cancer biology, and are of great interest in biomathematics. (
  • 6 Division of Pulmonary, Allergy and Critical Care Medicine, Program in Protease and Matrix Biology, Gregory Fleming James Cystic Fibrosis Centre and Lung Health Center, University of Alabama at Birmingham, Birmingham, AL 35294, USA. (
  • Kohn JC, Zhou DW, Bordeleau F, Zhou AL, Mason BN, Mitchell MJ, King MR, Reinhart-King CA. Cooperative effects of matrix stiffness and fluid shear stress on endothelial cell behavior. (
  • The animal extracellular matrix includes the interstitial matrix and the basement membrane . (
  • Second, I'll talk about the skin ECM, which comprises a basement membrane and interstitial matrix. (
  • Extracellular matrix (ECM) exists in two forms: interstitial matrix that fills in the intercellular space and the more specialized basement membrane, which is a thin sheet of extracellular matrix underlying the epithelium. (
  • The extracellular matrix (ECM) is an insoluble network of collagens, glycoproteins, proteoglycans and glycosaminoglycans. (
  • Extracellular matrix (ECM) contains collagen and elastic fibers embedded in a viscoelastic gel consisting of proteoglycans (PGs), hyaluronan and glycoproteins ( 29 ). (
  • MAPTrix™ produces a uniform ECM surface that provides a highly controlled 2D extracellular microenvironment for cell cultures and related applications. (
  • A promising tool for the in vitro exploration of extracellular microenvironment (ECM) factors that regulate cell functions is the combinatorial library approach. (
  • Matrix metalloproteinase-2 is required for the switch to the angiogenic phenotype in a tumor model," Proceedings of the National Academy of Sciences of the United States of America , vol. 97, no. 8, pp. 3884-3889, 2000. (
  • Similar results were found for matrix metalloproteinase (MMP)-3 (765 vs 370 pg·mL −1 , p=0.004). (
  • The molecular mechanism of SCCRO-mediated effects on matrix metalloproteinase-2 (MMP2) levels and activity were assessed using a combination of cell biological and molecular methods, including real-time PCR, reporter assay, RNA interference, and chromatin immunoprecipitation assay. (
  • Matrix metalloproteinase-9 was unrelated to either outcome. (
  • Matrix, extracellular phosphoglycoprotein with ASARM motif (bone) , also known as MEPE , is a human gene . (
  • Greenhouse DG, Murphy A, Mignatti P, Zavadil J, Galloway AC, Balsam LB. Mitral valve prolapse is associated with altered extracellular matrix gene expression patterns. (
  • The resulting cell-matrix reciprocity further affected adhesion, proliferation, and migration behavior of MSCs. (
  • We aimed to characterize and determine the importance of extracellular matrix (ECM) dynamics in cell migration during endometrial repair. (
  • Cell adhesion mediates cell attachment, migration, and signaling to and from the extracellular matrix. (
  • Microarray analysis revealed that in addition to modulating many of the same DC maturation genes as LPS, galectin-1 also uniquely up-regulated a significant subset of genes related to cell migration through the extracellular matrix (ECM). (
  • Corneal epithelium removed from underlying extracellular matrix (ECM) extends numerous cytoplasmic processes (blebs) from the formerly smooth basal surface. (
  • We further demonstrate in vitro production of SOM and extracellular mineralized particles in cell cultures of M. digitata . (
  • reported that in vitro a specialized brevican-containing matrix surrounds the AIS. (
  • Lethias C, Descollonges Y, Boutillon MM, Garrone R (1996) Flexilin: a new extracellular matrix glycoprotein localized on collagen fibrils. (
  • In this project we analyze effects of extracellular cues for hypertrophic remodeling and examine transcription factor circuits regulating metabolic reprogramming. (
  • These structural elements form from the same mesodermal primordium, develop in similar ways, have a core leather-like matrix of collagen, and are as responsive - subject to Wolffe's Law of remodeling - to sustained forces going through the body as is the rest of the fascia. (
  • Extracellular matrix remodeling and its contribution to protective adaptation following lengthening contractions in human muscle. (
  • 9 Matrix remodeling may therefore promote an activated EC phenotype in these regions. (
  • Background- Remodeling of the extracellular matrix (ECM) is a key aspect of myocardial response to biomechanical stress and heart failure. (
  • Objective- To evaluate if biomarkers reflecting left ventricular/vascular extracellular matrix remodeling are associated with cardiovascular disease (CVD) and death in the community. (
  • Extracellular matrix (ECM) turnover is an integral component of cardiovascular remodeling. (
  • 3. A method of treating a disease associated with extracellular matrix (ECM) accumulation in a patient, the method comprising administering to the patient a therapeutically effective amount of an agent capable of shifting ECM homeostasis from glycolysis to fatty acid oxidation (FAO). (
  • 7. A method of increasing extracellular matrix (ECM) accumulation in a patient, the method comprising administering to the patient a therapeutic agent capable of shifting ECM homeostasis from fatty acid oxidation (FAO) to glycolysis. (
  • In this minireview, we will examine key extracellular matrix and matricellular components, their changes with aging, and discuss how these alterations might influence the subsequent progression of cancer in the aged host. (
  • Dynamic alterations in extracellular matrix expression are essential for endometrial repair. (
  • Furthermore, water permeation through aquaporin-1 mediates the EV deformability, which further supports EV transport in hydrogels and a decellularized matrix. (
  • Fig. 4: Aquaporin-1 mediates the ability of EVs to transport in engineered and decellularized matrices by increasing the EV deformability. (
  • The brain's extracellular matrix (ECM) mediates structural stability by enwrapping synaptic contacts fundamental for long-term memory storage. (
  • For instance, matrices are filled with polymerized glycosaminoglycans, or GAGs for short. (
  • Today I'd like to talk about several of the extracellular matrices, ECM in mammalian skin. (
  • A return to an immature, more soluble matrix during tumor growth could facilitate tumor cell motility and angiogenesis. (
  • This study examines the effect of soluble extracellular matrix components on the basal surface. (
  • During brain maturation, the occurrence of the extracellular matrix (ECM) terminates juvenile plasticity by mediating structural stability. (
  • Together with other studies, these data elucidate a network of matrix-specific pathways that determine inflammatory events in response to fluid shear stress. (
  • The nerve fibers of the optic nerve are enclosed and segmented by extracellular matrix. (
  • The extracellular matrix consists of fibers and a basic compound. (
  • 6 ]. It must be noted that tenocytes require a long time to produce a dense, collagen-rich neo-matrix consisting of parallel aligned type I collagen fibers. (
  • AMSBIO supplies M ussel A dhesive P rotein based ma trix ( MAPTrix TM ) recombinant extracellular matrix (ECM) that act as biometic mimics for traditional basement membrane extracts. (
  • Employing optical spectroscopy we have performed a comparative study of the dielectric response of extracellular matrix and filaments of electrogenic bacteria Shewanella oneidensis MR-1, cytochrome c, and bovine serum albumin. (
  • The benefits of matrix organization are said to be that it facilitates interdepartmental coordination during innovation, and, by weakening departmental boundaries, encourages greater flexibility and creativity. (
  • Extracellular matrix proteinases play an important role in the pathogenesis of RSV bronchiolitis. (