Dental Enamel Proteins: The proteins that are part of the dental enamel matrix.Dental Enamel: A hard thin translucent layer of calcified substance which envelops and protects the dentin of the crown of the tooth. It is the hardest substance in the body and is almost entirely composed of calcium salts. Under the microscope, it is composed of thin rods (enamel prisms) held together by cementing substance, and surrounded by an enamel sheath. (From Jablonski, Dictionary of Dentistry, 1992, p286)Amelogenin: A major dental enamel-forming protein found in mammals. In humans the protein is encoded by GENES found on both the X CHROMOSOME and the Y CHROMOSOME.Amelogenesis: The elaboration of dental enamel by ameloblasts, beginning with its participation in the formation of the dentino-enamel junction to the production of the matrix for the enamel prisms and interprismatic substance. (Jablonski, Dictionary of Dentistry, 1992).Dental Enamel Hypoplasia: An acquired or hereditary condition due to deficiency in the formation of tooth enamel (AMELOGENESIS). It is usually characterized by defective, thin, or malformed DENTAL ENAMEL. Risk factors for enamel hypoplasia include gene mutations, nutritional deficiencies, diseases, and environmental factors.Ameloblasts: Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)Enamel Organ: Epithelial cells surrounding the dental papilla and differentiated into three layers: the inner enamel epithelium, consisting of ameloblasts which eventually form the enamel, and the enamel pulp and external enamel epithelium, both of which atrophy and disappear before and upon eruption of the tooth, respectively.Matrix Metalloproteinase 20: A secreted matrix metalloproteinase that is the predominant proteolytic activity in the enamel matrix. The enzyme has a high specificity for dental enamel matrix protein AMELOGENIN.Tooth Germ: The collective tissues from which an entire tooth is formed, including the DENTAL SAC; ENAMEL ORGAN; and DENTAL PAPILLA. (From Jablonski, Dictionary of Dentistry, 1992)Dental Enamel Permeability: The property of dental enamel to permit passage of light, heat, gases, liquids, metabolites, mineral ions and other substances. It does not include the penetration of the dental enamel by microorganisms.Tooth Calcification: The process whereby calcium salts are deposited in the dental enamel. The process is normal in the development of bones and teeth. (Boucher's Clinical Dental Terminology, 4th ed, p43)Amelogenesis Imperfecta: A clinically and genetically heterogeneous group of hereditary conditions characterized by malformed DENTAL ENAMEL, usually involving DENTAL ENAMEL HYPOPLASIA and/or TOOTH HYPOMINERALIZATION.Tooth Demineralization: A tooth's loss of minerals, such as calcium in hydroxyapatite from the tooth matrix, caused by acidic exposure. An example of the occurrence of demineralization is in the formation of dental caries.Dental Caries: Localized destruction of the tooth surface initiated by decalcification of the enamel followed by enzymatic lysis of organic structures and leading to cavity formation. If left unchecked, the cavity may penetrate the enamel and dentin and reach the pulp.Dental Enamel Solubility: The susceptibility of the DENTAL ENAMEL to dissolution.Dental Care: The total of dental diagnostic, preventive, and restorative services provided to meet the needs of a patient (from Illustrated Dictionary of Dentistry, 1982).Hardness: The mechanical property of material that determines its resistance to force. HARDNESS TESTS measure this property.Tooth Remineralization: Therapeutic technique for replacement of minerals in partially decalcified teeth.Tooth Bleaching: The use of a chemical oxidizing agent to whiten TEETH. In some procedures the oxidation process is activated by the use of heat or light.Tooth Erosion: Progressive loss of the hard substance of a tooth by chemical processes that do not involve bacterial action. (Jablonski, Dictionary of Dentistry, 1992, p296)Tooth Discoloration: Any change in the hue, color, or translucency of a tooth due to any cause. Restorative filling materials, drugs (both topical and systemic), pulpal necrosis, or hemorrhage may be responsible. (Jablonski, Dictionary of Dentistry, 1992, p253)Hardness Tests: A test to determine the relative hardness of a metal, mineral, or other material according to one of several scales, such as Brinell, Mohs, Rockwell, Vickers, or Shore. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Tooth: One of a set of bone-like structures in the mouth used for biting and chewing.Cariostatic Agents: Substances that inhibit or arrest DENTAL CARIES formation. (Boucher's Clinical Dental Terminology, 4th ed)Molar: The most posterior teeth on either side of the jaw, totaling eight in the deciduous dentition (2 on each side, upper and lower), and usually 12 in the permanent dentition (three on each side, upper and lower). They are grinding teeth, having large crowns and broad chewing surfaces. (Jablonski, Dictionary of Dentistry, 1992, p821)Fluorides: Inorganic salts of hydrofluoric acid, HF, in which the fluorine atom is in the -1 oxidation state. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Sodium and stannous salts are commonly used in dentifrices.Education, Dental: Use for articles concerning dental education in general.Fluorosis, Dental: A chronic endemic form of hypoplasia of the dental enamel caused by drinking water with a high fluorine content during the time of tooth formation, and characterized by defective calcification that gives a white chalky appearance to the enamel, which gradually undergoes brown discoloration. (Jablonski's Dictionary of Dentistry, 1992, p286)Schools, Dental: Educational institutions for individuals specializing in the field of dentistry.Incisor: Any of the eight frontal teeth (four maxillary and four mandibular) having a sharp incisal edge for cutting food and a single root, which occurs in man both as a deciduous and a permanent tooth. (Jablonski, Dictionary of Dentistry, 1992, p820)Students, Dental: Individuals enrolled a school of dentistry or a formal educational program in leading to a degree in dentistry.Tooth Bleaching Agents: Chemicals that are used to oxidize pigments in TEETH and thus effect whitening.Fluoride PoisoningFluorides, Topical: Fluorides, usually in pastes or gels, used for topical application to reduce the incidence of DENTAL CARIES.Dental Care for Chronically Ill: Dental care for patients with chronic diseases. These diseases include chronic cardiovascular, endocrinologic, hematologic, immunologic, neoplastic, and renal diseases. The concept does not include dental care for the mentally or physically disabled which is DENTAL CARE FOR DISABLED.Durapatite: The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.Dental Pulp: A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)Dental Care for Children: The giving of attention to the special dental needs of children, including the prevention of tooth diseases and instruction in dental hygiene and dental health. The dental care may include the services provided by dental specialists.Dental Clinics: Facilities where dental care is provided to patients.Sodium Fluoride: A source of inorganic fluoride which is used topically to prevent dental caries.Dental Hygienists: Persons trained in an accredited school or dental college and licensed by the state in which they reside to provide dental prophylaxis under the direction of a licensed dentist.Faculty, Dental: The teaching staff and members of the administrative staff having academic rank in a dental school.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Dental Care for Disabled: Dental care for the emotionally, mentally, or physically disabled patient. It does not include dental care for the chronically ill ( = DENTAL CARE FOR CHRONICALLY ILL).Dental Anxiety: Abnormal fear or dread of visiting the dentist for preventive care or therapy and unwarranted anxiety over dental procedures.Insurance, Dental: Insurance providing coverage for dental care.Dental Research: The study of laws, theories, and hypotheses through a systematic examination of pertinent facts and their interpretation in the field of dentistry. (From Jablonski, Illustrated Dictionary of Dentistry, 1982, p674)Dental Auxiliaries: Personnel whose work is prescribed and supervised by the dentist.Dental Health Services: Services designed to promote, maintain, or restore dental health.Dental Cementum: The bonelike rigid connective tissue covering the root of a tooth from the cementoenamel junction to the apex and lining the apex of the root canal, also assisting in tooth support by serving as attachment structures for the periodontal ligament. (Jablonski, Dictionary of Dentistry, 1992)Tooth Root: The part of a tooth from the neck to the apex, embedded in the alveolar process and covered with cementum. A root may be single or divided into several branches, usually identified by their relative position, e.g., lingual root or buccal root. Single-rooted teeth include mandibular first and second premolars and the maxillary second premolar teeth. The maxillary first premolar has two roots in most cases. Maxillary molars have three roots. (Jablonski, Dictionary of Dentistry, 1992, p690)Cementogenesis: The formation of DENTAL CEMENTUM, a bone-like material that covers the root of the tooth.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Periodontal Ligament: The fibrous CONNECTIVE TISSUE surrounding the TOOTH ROOT, separating it from and attaching it to the alveolar bone (ALVEOLAR PROCESS).
Brooks, SJ, et al. Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Arch Oral Bio 1995;40 ... He J, et al. Emdogain promotes osteoblast proliferation and differentiation and stimulates osteoprotegerin expression. Oral ... Enamel matrix protein and its derivative. Making use of this finding, enamel matrix derivative (EMD) was introduced in ... a b Sculean A, et al. The application of an enamel matrix protein derivative (Emdogain) in regenerative periodontal therapy: A ...
Other significant proteins in enamel are amelogenins, enamelins, and ameloblastins. The human encoding gene for tuftelin (TUFT1 ... Dany Deutsch and Aharon Palmon from the Hebrew University-Hadassah School of Dental Medicine in Jerusalem. Tuftelin has been ... 2003). "The human tuftelin gene and the expression of tuftelin in mineralizing and nonmineralizing tissues". Connect. Tissue ... Tuftelin is an acidic phosphorylated glycoprotein found in tooth enamel. In humans, the Tuftelin protein is encoded by the ...
Although less than 5% of enamel consists of protein, ameloblastins comprise 5-10% of all enamel protein. This protein is formed ... Bartlett JD, Simmer JP (1999). "Proteinases in developing dental enamel". Critical Reviews in Oral Biology and Medicine. 10 (4 ... Lee SK, Krebsbach PH, Matsuki Y, Nanci A, Yamada KM, Yamada Y (December 1996). "Ameloblastin expression in rat incisors and ... This gene encodes the nonamelogenin enamel matrix protein ameloblastin. The encoded protein may be important in enamel matrix ...
Several gene expression is needed for enamel formation where the relevant matrix proteins & proteinases are transcribed for ... Simmer JP, Hu JC (2001). "Dental enamel formation and its impact on clinical dentistry". J Dent Educ. 65 (9): 896-905. PMID ... Enamel of abnormal thickness due to malfunction in enamel matrix formation. Enamel is very thin but hard & translucent, and may ... Enamel defect due to malfunction of enamel calcification, therefore enamel is of normal thickness but is extremely brittle, ...
... and contains more protein and water. In general, MMP-20 functions in enamel are to cleave enamel matrix proteins at specific ... Bartlett JD, Simmer JP (2000). "Proteinases in developing dental enamel". Crit. Rev. Oral Biol. Med. 10 (4): 425-441. doi: ... 2001). "Expression and regulation of MMP-20 in human tongue carcinoma cells". J. Dent. Res. 80 (10): 1884-1889. doi:10.1177/ ... "Entrez Gene: MMP20 matrix metallopeptidase 20 (enamelysin)". Moradian-Oldak J (2012). "Protein-mediated enamel mineralization ...
The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation ... and cloning of enamel matrix serine proteinase 1". Journal of dental research. 77 (2): 377-86. doi:10.1177/00220345980770020601 ... of enamel proteins. Alternate splice variants for this gene have been described, but their biological validity has not been ... 2001). "Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region". Gene. 257 ( ...
Mutations in enamel ECM proteins result in enamel defects such as amelogenesis imperfect. Type-I collagen is thought to have a ... Dental enamel mineral (as well as dentin and bone) is made of hydroxylapatite with foreign ions incorporated in the structure. ... February 2011). "Expression of phosphophoryn is sufficient for the induction of matrix mineralization by mammalian cells". The ... Proteins of the developing enamel extracellular matrix (such as Amelogenin) control initial mineral deposition (nucleation) and ...
... elin is a protein that in humans is encoded by the ENAM gene. Dental enamel is a highly mineralized tissue with 85% of its ... 2000). "Cloning human enamelin cDNA, chromosomal localization, and analysis of expression during tooth development". J. Dent. ... Enamelin is the largest protein in the enamel matrix of developing teeth and comprises approximately 5% of total enamel matrix ... 2001). "Mutation of the gene encoding the enamel-specific protein, enamelin, causes autosomal-dominant amelogenesis imperfecta ...
Salido EC, Yen PH, Koprivnikar K, Yu LC, Shapiro LJ (Feb 1992). "The human enamel protein gene amelogenin is expressed from ... "Human and mouse enamel phenotypes resulting from mutation or altered expression of AMEL, ENAM, MMP20 and KLK4". Cells, Tissues ... "Partial rescue of the amelogenin null dental enamel phenotype". The Journal of Biological Chemistry. 283 (22): 15056-15062. doi ... The protein Amelogenin, X isoform is an isoform of amelogenin that comes from the X chromosome. The protein Amelogenin is a ...
... at the interface between the inner enamel epithelium and dental papilla, leaving behind the odontoblastic process within the ... Maurin JC, Couble ML, Didier-Bazes M, Brisson C, Magloire H, Bleicher F (August 2004). "Expression and localization of reelin ... It has been shown that odontoblasts secrete the extracellular matrix protein reelin. A pulpal A-delta (noxious, short sharp ... during dental procedures). Odontoblasts were originally the outer cells of the dental papilla. Thus, dentin and pulp tissue ...
... is expressed in enamel knots, where it regulates gene expression and cell growth. The gene modulates p21-mediated ... Growth arrest and DNA-damage-inducible protein GADD45 gamma is a protein that in humans is encoded by the GADD45G gene on ... "Gadd45g regulates dental epithelial cell proliferation through p38 MAPK-mediated p21 expression". Genes Cells. 18 (8): 660-71. ... There is a low expression due to methylation of the GADD45G promotor. This low expression can also be explained by increased NF ...
If oral bacteria ferment carbohydrates and produce acids that may dissolve tooth enamel and induce dental decay, then sweetened ... In the 1970s, the Supreme Court ruled that advertising was not a form of free expression, but a form of business practices ... protein and other micronutrients. Phosphorus, a micronutrient, can be found in cola-type beverages, but there may be a risk in ... Drinking acidic drinks over a long period and continuous sipping may erode the tooth enamel. A 2007 study determined that some ...
Gene and protein expressionEdit. About 20,000 protein coding genes are expressed in human cells and 60% of these genes are ... "Sunningdale Dental News & Views. Retrieved 25 February 2018.. *^ Taylor, John. "Immunity in the oral cavity". British Society ... Demineralization occurs when enamel disintegrates due to the presence of acid. When this occurs, the buffering capacity effect ... are salivary gland specific proteins with highest level of expression. Examples of other specifically expressed proteins ...
Gualtieri, G.; Colacicchia, S.; Sgattonic, R.; Giannonic, M. (2001). "The Chernobyl Accident: EPR Dosimetry on Dental Enamel of ... Yashroy, R. C. (1991). "Protein heat denaturation and study of membrane lipid-protein interactions by spin label ESR". Journal ... the position of the EPR resonance is given by the expression gxxBx + gyyBy + gzzBz. Here Bx, By and Bz are the components of ... Radiation damage over long periods of time creates free radicals in tooth enamel, which can then be examined by EPR and, after ...
... may be caused by dental caries that penetrate through the enamel and dentin to reach the pulp, or it may be a result ... As a result, an increased co-expression of multiple isoforms at demyelinating nodal sites in painful dental pulp. This isoforms ... The biological effects of SP are expressed by the binding of specific G protein-coupled NK receptors. Interaction with SP ... In addition, dental caries is more likely to develop pulpitis due to less time for the dental pulp to react and protect itself ...
... may be caused by dental caries that penetrate through the enamel and dentin to reach the pulp, or it may be a result ... As a result, an increased co-expression of multiple isoforms at demyelinating nodal sites in painful dental pulp. This isoforms ... The biological effects of SP are expressed by the binding of specific G protein-coupled NK receptors. Interaction with SP ... Seltzer and Bender's Dental Pulp. Quintessence, 2002. *^ a b c d e Eugene Chen and Paul V. Abbott, "Dental Pulp Testing: A ...
... the presumed fluoride exporter CrcB and a superfamily of CLC membrane proteins called Eric^F proteins. The CLC^F proteins have ... The fluoride acts as a hardening agent with the enamel base on teeth, remineralizing and protecting them from harsh acids and ... Of particular interest is Streptococcus mutans, a major cause of dental caries. It has been shown that sodium fluoride has ... These "fluoride riboswitches" increase expression of downstream genes when fluoride levels are elevated, and the genes are ...
... , a Golgi localized protein kinase, is a serine kinase that phosphorylates both casein and other highly acidic proteins ... Wang X, Hao J, Xie Y, Sun Y, Hernandez B, Yamoah AK, Prasad M, Zhu Q, Feng JQ, Qin C (Nov 2010). "Expression of FAM20C in the ... Journal of Dental Research. 92 (11): 995-9. doi:10.1177/0022034513504588. PMC 3797537 . PMID 24026952. Wang X, Wang J, Liu Y, ... while their dentin and enamel defects are largely independent from the hypophosphatemia and appear to be a local effects of ...
The function of this protein is not currently known. Expression studies have shown that the gene has high expression in the ... The enamel is thin, rough, and prone to crumbling. Two types of amelogenesis imperfecta (AI) have been seen in KTS patients. ... Part 2: Syndromes with significant dental involvement". European Journal of Medical Genetics. 51 (5): 383-408. doi:10.1016/j. ... Protein staining has provided evidence supporting that the RODGI protein may be a part of the nuclear envelope. Studies also ...
... dental irregularities and defective tooth enamel, as well as hormone problems, the most common being high blood calcium. ... Researchers have found this hemizygosity for the ELN gene, which codes for the protein elastin, is associated with the ... There is also evidence that individuals with Williams exhibit hyper amygdala activity when viewing happy facial expressions. ... "Submicroscopic Deletion in Patients with Williams-Beuren Syndrome Influences Expression Levels of the Nonhemizygous Flanking ...
... dental enamel, nails, lens, and the internal ear. Neuroectodermal derivatives that can be affected include sensory placodes, ... The mutations found in EEC are missense mutations, meaning that there is a single amino acid change in the protein, as opposed ... Though we can calculate the chance of inheritance of the gene, the degree of expression cannot be calculated. Genetics research ... Articulation is further impaired by the numerous dental anomalies, including missing or malformed teeth found in EEC syndrome. ...
Both the Fas and Fas ligand proteins are overexpressed in primary SS patients, while expression of BCL-1, which is known to ... Treatments include at-home topical fluoride application to strengthen tooth enamel and frequent teeth cleanings by a dental ... Dental careEdit. Preventive dental treatment is also necessary (and often overlooked by the patient), as the lack of saliva ... Viral proteins, engulfed molecules, or degraded self-structures may initiate autoimmunity by molecular mimicry and increase the ...
Dental pattern in primates vary considerably; although some have lost most of their incisors, all retain at least one lower ... For example, the grey-cheeked mangabey has thick enamel on its teeth, enabling it to open hard fruits and seeds that other ... Data for some African cities show that half of all protein consumed in urban areas comes from the bushmeat trade. Endangered ... Recent studies involve comparing DNA to examine both the variation in the expression of the dimorphism among primates and the ...
Source: "Protein Energy Malnutrition". Cognitive development. Protein-calorie malnutrition can cause cognitive ... Enamel mottling, delayed eruption. Hair. Dull, sparse, brittle hair, hypopigmentation, flag sign (alternating bands of light ... Poor dental health, ill-fitting dentures, or chewing and swallowing problems can make eating difficult. As a result of ... Conditions are characterized by extreme wasting of the muscles and a gaunt expression. ...
... dental enamel defects and with diagnose of Down syndrome or Turner syndrome. ... Coeliac disease is caused by a reaction to gliadins and glutenins (gluten proteins) found in wheat, and similar proteins ... "Multiple common variants for celiac disease influencing immune gene expression". Nature Genetics. 42 (4): 295-302. doi:10.1038 ... These are storage proteins rich in proline (prol-) and glutamine (-amin) that dissolve in alcohols and are resistant to ...
If oral bacteria ferment carbohydrates and produce acids that may dissolve tooth enamel and induce dental decay, then sweetened ... In the 1970s, the Supreme Court ruled that advertising was not a form of free expression, but a form of business practices ... protein and other micronutrients. Phosphorus, a micronutrient, can be found in cola-type drinks, but there may be a risk in ... Drinking acidic drinks over a long period and continuous sipping may erode the tooth enamel. A 2007 study determined that some ...
N2 - Amelogenin is the most abundant protein of the enamel organic matrix and is a structural protein indispensable for enamel ... AB - Amelogenin is the most abundant protein of the enamel organic matrix and is a structural protein indispensable for enamel ... Amelogenin is the most abundant protein of the enamel organic matrix and is a structural protein indispensable for enamel ... "Amelogenin is the most abundant protein of the enamel organic matrix and is a structural protein indispensable for enamel ...
... suggesting that unknown genes/proteins that are critical for dental enamel formation. Our results indicate that additional ... Simmer JP, Hu JC: Expression, structure, and function of enamel proteinases. Connect Tissue Res. 2002, 43 (2-3): 441-449.View ... Amelogenin is the most abundant protein in developing enamel, accounting for more than 90% of total enamel protein , while ... Enamel findings in AI are highly variable, ranging from deficient enamel formation to defects in the mineral and protein ...
Dental Enamel Proteins • Dentition • Development • Diet • Electromyography • Electrophoresis, Polyacrylamide Gel • Feeding ... to study variation in the expression patterns of a number of diet-related genes in chimpanzees and humans; current work in ... genomic study of genes that code for enamel matrix proteins and associated proteases to document whether the thick enamel trait ... Sodium-Phosphate Cotransporter Proteins, Type III • Species Specificity • Strepsirhini • Stress, Mechanical • Sucking Behavior ...
... proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium ... Protein Coding), Chromosome 6 Open Reading Frame 58, including: function, ... Tissue specificity: Detected in saliva and in hypomineralized dental enamel (at protein level). ... Protein Expression for C6orf58 Gene. See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB ...
Effect of enamel matrix proteins on the phenotype expression of periodontal ligament cells cultured on dental materials. J ... Effect of enamel matrix proteins on the periodontal connective tissue-material interface after wound healing. J Biomed Mater ... Box I and II motif from myelin basic protein gene promoter binds to nuclear proteins from rodent brain. J Mol Neurosci. 1994; 5 ... Box I and II motif from myelin basic protein gene promoter binds specifically to nuclear proteins from rodent brain. Journal of ...
Biochemistry and molecular biology of amelogenin proteins of developing dental enamel. Arch Oral Bio 1995;40:1-14 Boyan, BD, et ... Emdogain promotes osteoblast proliferation and differentiation and stimulates osteoprotegerin expression. Oral Surg Oral Med ... Based on the high degree of homology between porcine and human enamel proteins, it was decided to produce the EMD from fetal ... This led researchers to conclude that enamel matrix proteins (or EMPs) laid down by Hertwigs epithelial root sheath serve as ...
Profile search lead to isolation of 87 genes with significantly similar expression to the three enamel proteins. These mRNAs ... were expressed in dental epithelium and epithelium derived cells. Although expression of Ambn, Amelx, and Enam were lower ... The expression of the three enamel genes was low at pre-natal stages (E11.5-P0) increasing after birth (P1-P7). ... The profile search function of Spotfire software was used to select genes with similar expression profile as the enamel genes ( ...
Protein Coding), Fibroblast Growth Factor 3, including: function, proteins, disorders, pathways, orthologs, and expression. ... Secondary Enamel Knot Cells Enamel Knot * Dental Papilla Cells Dental Papilla * Primary Enamel Knot Cells Enamel Knot ... Protein Expression for FGF3 Gene. See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB ... Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for FGF3 Gene. ...
The protein encoded by this gene degrades amelogenin the major protein component of dental enamel matrix and thus thought to ... PrimePCR™ ddPCR™ Expression EvaGreen® Assay: MMP20, Human. print ddPCR™ Evagreen assay for gene expression analysis. EvaGreen ... PrimePCR™ ddPCR™ Gene Expression Assays (EvaGreen) Product Insert, Rev A. Click to download ... PrimePCR™ ddPCR™ Gene Expression Probe Assays Product Insert, Rev A. Click to download ...
This expression pattern strongly differs from the spatiotemporal expression of other ameloblast-secreted SCPPs, such as the ... in contrast to its expression limited to enamel maturation in rodents. Taken together our findings suggest that AMTN was ... enamel matrix proteins (EMPs). Furthermore, AMTN was characterized in rodents only. In this study, we applied various ... These changes likely led to a partial loss of AMTN function and could have a link with the emergence of prismatic enamel in ...
Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to ... Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to ... Characterization of enamel and dentin protein expression in bioengineered human tooth tissues Poster Feb 18, 2010 ... This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At ...
The photolabile protein nanoprobes expression optimization of human structural dental enamel proteins. bachelor thesis. Author ... Dental enamel is the hardest tissue of the body. It is formed by an evolutionarily highly conserved biomineralization process ... Proteomická studie spojovací vrstvy dentin-enamel. Proteomic study of the dentin-enamel junction layer.. bachelor thesis. ... expression and purification of recombinant protein (2)gas chromatography (2)... View More ...
J) Immunohistochemical analysis showed p63 protein expression throughout the oral and dental epithelia at E11. (K) Expression ... L) In the P1 incisor, p63 protein expression was most intense in the outer enamel epithelium. (M) Western blot analysis of p63 ... To confirm the presence of the dental lamina in the mutants, we analysed the expression of two dental lamina markers Pitx2 and ... E) At the bell stage, p63 expression was particularly intense in the outer enamel epithelium and weaker in the inner enamel ...
We retrieved dental enamel proteome sequences from a 1.9-million-year-old G. blacki molar found in Chuifeng Cave, China7,8. The ... In addition, we hypothesize that the expression of alpha-2-HS-glycoprotein, which has not been previously observed in enamel ... thermal age of these protein sequences is approximately five times greater than that of any previously published mammalian ... The survival of an Early Pleistocene dental enamel proteome in the subtropics further expands the scope of palaeoproteomic ...
In contrast to Fam20C, which is ubiquitously expressed, Fam20A expression has only been detected in dental tissues, lactating ... Fam20C phosphorylates the enamel matrix proteins within SxE motifs.. (A) Phosphorylation of enamel matrix proteins by Fam20C in ... Fam20C phosphorylates enamel matrix proteins in vitro and in ameloblasts. To determine whether enamel matrix proteins are ... Protein expression and purification. MBP-tagged Fam20A and Fam20C proteins were expressed in Hi5 insect cells and purified from ...
Similar expression patterns of these proteins were observed in incisors containing all differentiation stages of the dental ... indicating accumulation of enamel matrix proteins. Cells surrounding focal areas are immunopositive for enamel matrix proteins ... indicating accumulation of enamel matrix proteins. Cells surrounding focal areas are immunopositive for enamel matrix proteins ... RT-PCR analysis of tumor tissue revealed expression of mRNA for enamel matrix proteins including amelogenin, enamelin, and ...
Enamel and dental anomalies in latent-transforming growth factor beta-binding protein 3 mutant mice. Opens in a new tab ... Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-beta Signaling in Human Bone Marrow Stromal Cells ... ß binding protein (LTBP). LTBPs are complex proteins consisting of multiple EGF-like domains and signature 8-cysteine- ... We have found that these proteins may participate in patterning of the dorsal ventral axes in early embryos. We think that this ...
In the developing enamel organ, gradients of fundamental protein expressions were produced being strong towards the oral ... Expression of desmosomal proteins and their implications during enamel organ morphogenesis. Hadi BILAL. July-September 2018, 10 ... Background/Objective: The present article is between the fewest that describe the expression of desmosomal proteins in human ... The dental, oral and ectodermal epithelia were investigated in 7 and 9 weeks human embryos. Results: The expression of these ...
Parathyroid hormone-related protein (PTHrP) expression in ameloblastoma. Rafik A Abdelsayed, Robert K. Vartanian, Kimberly K. ... Fingerprint Dive into the research topics of Parathyroid hormone-related protein (PTHrP) expression in ameloblastoma. ...
expression of KLK4 correlates with the stage-associated changes in the digestion of enamel proteins ... Both KLK4 and AMTN proved to be essential for the maturation of dental enamel, a process that requires the removal of ... The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation ... Klk4 is essential for the removal of enamel proteins and the proper maturation of enamel crystals in mice ...
... caused by FAM20A mutation display several dental phenotypes including hypoplastic enamel, intrapulpal calcification, delayed ... The expression and localization of Fam20a protein were examined using anti-Fam20a antibody by immunofluorescence-based staining ... The IP-WB analysis was performed using cultured media and cell lysates from LS8 cells and the protein expression was clearly ... Goldman School of Dental Medicine, Boston, MA, 2Department of Oral Anatomy, Osaka Dental University, Osaka, Japan, 3University ...
Pro-apoptotic protein Bax expression was elevated in the heavy-force group, whereas anti-apoptotic protein Bcl-2 expression was ... in particular the dental follicle and inner enamel epithelium. The descendants of these structures include the periodontal ... RANK expression on days 7 and 21 and RANKL expression on day 21 differed between the force groups. CONCLUSIONS: Evidence of ... Expression of Bax, Bcl-2, caspase 3, caspase 9, and RANK-RANKL were assessed by immunohistochemistry. Expression levels at the ...
Expression patterns of the shell matrix protein genes MSI31 and MSI60 in the pearl sac epithelium were examined by in situ ... enamel reconstruction is a significant topic in material science and dentistry as a novel approach for the treatment of dental ... Pearl microstructure and expression of shell matrix protein genes MSI31 and MSI60 in the pearl sac epithelium of Pinctada ... Expression data needs to be confirmed and complemented with expression patterns using e.g. in situ hybridization, a technique ...
Regrowing enamel? USC Dental Professor Janet Moradian-Oldak is on the case ... Oral and Dental Health. 03/22/2019 Oral and Dental Health New class of membranes shown to regenerate tissue and bone, viable ... Oral and Dental Health*Palliative Care*Pregnancy and Childbirth*Breast Feeding. *Sexual Health*Skin, Hair and Nails*Sports ... To help biologists in the quest to know more, a team of Stanford bioengineers has designed a suite of protein motors that can ...
Regrowing enamel? USC Dental Professor Janet Moradian-Oldak is on the case ... Oral and Dental Health. 03/22/2019 Oral and Dental Health New class of membranes shown to regenerate tissue and bone, viable ... In contrast, PROTACs engage the cells own protein degradation machinery to destroy targeted proteins by tagging them for ... Oral and Dental Health*Palliative Care*Pregnancy and Childbirth*Breast Feeding. *Sexual Health*Skin, Hair and Nails*Sports ...
AmelogeninGenesCellsProteinasesAmelogenesis imperfectaSecretionEpitheliumAmeloblastsGenesGeneCariesTooth developmentAmeloblastinKnotEpithelial cellsRecombinant ProteinsDepositionHypomineralizationSecretory stageAmeloblastMaturation stageMiceMRNAPhosphorylationBoneEnamelinMatrix derivativeSalivaryPeriodontalTissueBiomineralizationHypoplasticFluorideSevere enamel hypoplasiaAffects enamelProliferationDemineralizationMutationsOdontoblastPrevalence of dental fluorosisEndoplasmic reticulum stressMorphogenesisLocalizationInducesMMP20
- Amelogenin is the most abundant protein of the enamel organic matrix and is a structural protein indispensable for enamel formation. (elsevier.com)
- The main structural proteins in forming enamel are amelogenin, ameloblastin, and enamelin. (biomedcentral.com)
- However, no cases of mutation in the Y-chromosome amelogenin gene have been reported [ 13 ], due to the fact that, the amino acid sequence of the X and Y chromosome amelogenin genes are not the same and only the X copy is critical for normal enamel development. (biomedcentral.com)
- Each family was also evaluated for linkage to chromosome regions known to contain genes important in enamel development. (biomedcentral.com)
- Different mutations in genes that encode principal matrix proteins and proteinases of enamel have been associated with the different phenotypes of AI. (biomedcentral.com)
- The chromosome 4q13 region contains at least 3 genes important in enamel development: enamelin, ameloblastin, and amelotin. (biomedcentral.com)
- Along the tooth base AMTN is still expressed in the cells of the inner dental epithelium. (biomedcentral.com)
- AMTN expression is visible in the inner dental epithelium cells facing the tooth matrix along the tooth base. (biomedcentral.com)
- ode: outer dental epithelium. (biomedcentral.com)
- Development starts from the dental lamina, which forms as a stripe of thickened epithelium at the site of the future dental arch. (biologists.org)
- The initiation of both molars and incisors becomes morphologically visible in the mouse at embryonic day 11 (E11) when the dental lamina epithelium thickens locally. (biologists.org)
- The dental placodes form from this epithelium at E12. (biologists.org)
- Expression patterns of the shell matrix protein genes MSI31 and MSI60 in the pearl sac epithelium were examined by in situ hybridization 38 days after implantation, and related to pearl quality. (jove.com)
- Cells of the inner enamel epithelium located adjacent to the dental papilla mesenchymal cells, differentiate into early ameloblasts during the bell stage. (lifemapsc.com)
- Independent validation using six expressed genes by immunohistochemical analysis demonstrated that the corresponding proteins are strongly expressed in the developing molar tooth germ, in particular the dental follicle and inner enamel epithelium. (bvsalud.org)
- The expression of BMPs transcripts in the dental epithelium, however, is specifically restricted both in space and time during prenatal development. (go.jp)
- The first step is early morphogenesis (EM) when a focal surface of a specialized epithelium (the dental lamina or odontogenic band) thickens and signals towards the mesenchymal compartment. (biomedcentral.com)
- Cell proliferation drives morphogenesis of the growing bud during the late morphogenesis step (LM, also named cap-stage in the mouse) and is characterized in mammals by the presence of a transient signaling center in the inner dental epithelium, named the primary enamel knot [ 17 ], which induces regionalized proliferation of both epithelial and mesenchymal cells leading to the first acquisition of the tooth bud shape. (biomedcentral.com)
- Protein was also localized in the epithelium. (helsinki.fi)
- While Dact2 expression was restricted to the dental epithelium including the enamel knot signaling centers and tooth specific preameloblasts, Dact1 and Dact3 showed developmentally regulated expression in the dental mesenchyme. (pubmedcentralcanada.ca)
- We have investigated their expression at mRNA level in the dental epithelium of the rat incisor, based on the differentiation stages. (nii.ac.jp)
- Our immunocytochemical examinations showed its expression throughout the dental epithelium during the amelobalst-differentiation. (nii.ac.jp)
- Abundant immunoreation for Shh was recognizable both in multi-layered stratum intermedium and its adjacent inner dental epithelium. (nii.ac.jp)
- Furthermore, Shh-immunoreation was distributed in the mesenchyme close to inner dental epithelium in a gradient manner. (nii.ac.jp)
- This research aimed to study and discuss the subject by the immunohistochemical detection of cytokeratins, laminin, collagen IV, PCNA and p53 in 8 tumor samples and 8 dental follicle samples containing reduced enamel epithelium. (scielo.br)
- After considerations about pathogenesis, the authors suggested that the nature of AOT is hamartomatous with histogenesis from the reduced enamel epithelium. (scielo.br)
- Tooth germ morphogenesis begins with a thickening of the epithelium to form the dental epithelium during the lamina and placode stages [ 2 ]. (ijbs.com)
- During the bud stage, the dental epithelium invaginates to form the dental mesenchyme and then progresses to the cap stage [ 2 ]. (ijbs.com)
- Dental epithelial cells consist of four distinct cell populations, including stellate reticulum (SR), outer enamel epithelium (OEE), Notch1 -expressing stratum intermedium (SI) and fibroblast growth factor 9 ( Fgf9 )- and homeobox, msh-like 2 ( Msx2 )-positive inner enamel epithelium (IEE) [ 4 - 7 ]. (ijbs.com)
- Tooth morphogenesis and cytodifferentiations are regulated by epithelium-mesenchymal interactions, and the basement membrane plays a role in mediating these interactions Type IV collagen, the major component of the dental basement membrane, is composed of three αchains. (nii.ac.jp)
- Expression pattern of odontogenic ameloblast-associated and amelotin during formation and regeneration of the junctional epithelium. (semanticscholar.org)
- This incompletely differentiated epithelium is formed initially by the fusion of the reduced enamel organ with the oral epithelium (OE). (semanticscholar.org)
- Comprehensive analysis of gene expression in the junctional epithelium by laser microdissection and microarray analysis. (semanticscholar.org)
- During the initiation stage, the oral epithelium thickens to form the dental placode at the site of future tooth. (lifemapsc.com)
- During the bell stage, ameloblasts , originating from the dental epithelium, and odontoblasts , originating from the mesenchymal dental papilla, are fully differentiated. (lifemapsc.com)
- Since it is known that 13 different members of the fibroblast growth factor, or Fgf, family act as signaling proteins between the two different tissues -- dental mesenchyme and oral epithelium -- during murine tooth development, Javed and colleagues looked for changes in Fgf ligands in the mice lacking Sp7. (eurekalert.org)
- The initial ingrowth of the dental epithelium and its associated dental mesenchyme gives rise to the tooth bud. (nih.gov)
- Growth, morphogenesis and differentiation of the epithelium and mesenchyme are coordinated by secreted signaling proteins. (nih.gov)
- We have addressed the role of Shh in the developing tooth in mouse by using a conditional allele to remove Shh activity shortly after ingrowth of the dental epithelium. (nih.gov)
- Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. (bionity.com)
- Enamel, which covers the crown, is formed by ameloblasts derived from the dental epithelium, which never regenerates after tooth eruption. (spandidos-publications.com)
- Tooth morphogenesis results from reciprocal interactions between oral epithelium and ectomesenchyme culminating in the formation of mineralized tissues, enamel, and dentin.We found that recombinant ameloblastin adhered specifically to ameloblasts and inhibited cell proliferation.Thus, ameloblastin is a cell adhesion molecule essential for amelogenesis, and it plays a role in maintaining the differentiation state of secretory stage ameloblasts by binding to ameloblasts and inhibiting proliferation. (nih.gov)
- Tooth morphogenesis results from reciprocal interactions between oral epithelium and ectomesenchyme culminating in the formation of mineralized tissues, enamel, and dentin. (nih.gov)
- In mutant tooth, the dental epithelium differentiated into enamel-secreting ameloblasts, but the cells were detached from the matrix and subsequently lost cell polarity, resumed proliferation, and formed multicell layers. (nih.gov)
- Expression of Msx2, p27, and p75 were deregulated in mutant ameloblasts, the phenotypes of which were reversed to undifferentiated epithelium. (nih.gov)
- The mutant mice developed an odontogenic tumor of dental epithelium origin. (nih.gov)
- Tissue recombination experiments showed that its expression in epithelium is regulated by mesenchyme-derived signals. (jax.org)
- In this study, we identified strong expressions of GM3 and LacCer in dental epithelium, which give rise to differentiation into enamel-secreting ameloblasts. (elsevier.com)
- Lef-1 is expressed in the dental epithelium after Pitx2 , and both factors have overlapping expression patterns in the tooth bud and cap stages. (biologists.org)
- Furthermore, the LEF-1 promoter is differentially activated by PITX2 isoforms, which are co-expressed in dental epithelium. (biologists.org)
- Ameloblasts are strongly labelled along the crown base where enamel was recently deposited while labelling is weaker at its top where enamel is well mineralized. (biomedcentral.com)
- AMTN is no longer detected in the ameloblasts around the crown, but is identified in the cells at the surface of the dentin shaft, in a region lacking enamel. (biomedcentral.com)
- A strong AMTN expression is observed in the ameloblasts both facing the mineralized enamel and the recently deposited enamel matrix along the tooth shaft. (biomedcentral.com)
- The ameloblasts are no longer labelled at the top of the tooth, where enamel is mature (empty space after demineralization), but are labelled in the cells facing the tooth matrix along the tooth shaft. (biomedcentral.com)
- Conclusion: We demonstrated the intracellular expression of FAM20A in LS8 ameloblasts. (umich.edu)
- During the pre-secretory stage, the early ameloblasts differentiate and initiate synthesis of the enamel-related proteins. (lifemapsc.com)
- Decreased expression and increased phosphorylation of Forkhead box o1 (Foxo1) in ameloblasts were observed both in vivo and in vitro when treated by fluoride. (springer.com)
- The present study aims to investigate the possible relationship between Foxo1 and enamel matrix proteinases, matrix metalloproteinase 20 (MMP20), and kallikrein 4 (KLK4), in NaF-treated ameloblasts. (springer.com)
- Le MH, Nakano Y, Abduweli Uyghurturk D, Zhu L, Den Besten PK (2017) Fluoride alters Klk4 expression in maturation ameloblasts through androgen and progesterone receptor signaling. (springer.com)
- To determine whether 4PBA protects ameloblasts from fluoride toxicity, we analyzed gene expression of Tgf -β 1, Bcl2 / Bax ratio and cytochrome-c release in vitro . (frontiersin.org)
- Furthermore, in situ hybridization histochemistry and immunocytochemistry confirmed the region-specific expression of Alpha-and Gamma-ENac in the ameloblasts layer. (nii.ac.jp)
- The results revealed that nuclear FOXO1 was mainly localized in the transition and the mature ameloblasts and exhibited weaker expression in the rats exposed to fluoride. (fluoridealert.org)
- Previously, we reported that fluoride induces ER-stress in ameloblasts responsible for enamel formation, suggesting that ER-stress plays a role in dental fluorosis. (elsevier.com)
- Increased protein levels of p-SIRT1, ATG5 and ATG8/LC3 were present in fluoride-treated rat maturation stage ameloblasts. (elsevier.com)
- A reduction in Dpysl4 gene expression in the tooth germ produced a loss of ameloblasts, resulting in the decrease of synthesis and secretion of enamel. (ijbs.com)
- The inhibition of Dpysl4 gene expression led to promotion of cell proliferation of inner enamel epithelial cells and inhibition of the differentiation of these cells into pre-ameloblasts, which was confirmed by analyzing cell polarization, columnar cell structure formation and the expression of ameloblast marker genes. (ijbs.com)
- In the bell stage of the tooth germ, epithelial and mesenchymal cells undergo stepwise histodifferentiation into enamel-secreting ameloblasts and dentin-secreting odontoblast cell lineages, respectively [ 2 ]. (ijbs.com)
- IEE cells differentiate into columnar pre-ameloblasts with their nuclei shifted away from the basement membrane and that express enamel proteins, including Ameloblastin ( Ambn ) [ 4 , 8 ]. (ijbs.com)
- Global gene expression analysis of SOCE-deficient ameloblasts revealed strong dysregulation of several pathways. (jci.org)
- Cytodifferentiation is followed by the secretion step (late bell stage), when ameloblasts and odontoblasts produce enamel and dentin, respectively, the two main matrix components of the tooth, forming a tooth crown. (lifemapsc.com)
- When the crown is formed, the tooth erupts, eliminating the ameloblasts overlying the enamel of the tooth crown. (lifemapsc.com)
- Similarly, ameloblasts line up side by side in a sheet outside the developing tooth to lay down enamel. (eurekalert.org)
- Coincident with this process, adjacent epithelial and mesenchymal cells differentiate into enamel-secreting ameloblasts and dentin-secreting odontoblasts, respectively. (nih.gov)
- During this process, epithelial cells differentiate into enamel-secreting ameloblasts. (nih.gov)
- Ameloblastin, an enamel matrix protein, is expressed by differentiating ameloblasts. (nih.gov)
- The basement membrane was present at the basal lamina underneath presecretory stage ameloblasts (preameloblasts), however, at the secretory stage they disappeared and the enamel matrix emerged with some overlapping in the intermediate stage (enamel layer indicated by dotted lines). (nih.gov)
- Expression patterns of integrin α6, a receptor of laminin, are similar to that of the basement membrane: it was localized at the basal layer of preameloblasts, but disappeared when preameloblasts differentiated into ameloblasts. (nih.gov)
- Jag2 is expressed in epithelial cells that give rise to the enamel-producing ameloblasts from the earliest stages of tooth development. (jax.org)
- Conclusions: The low pH environment of maturation stage ameloblasts facilitates the uptake of \(F^−\), causing increased cell stress that compromises ameloblast function, resulting in dental fluorosis. (harvard.edu)
- Taken together, these results suggest that GM3 and LacCer are essential for NT-4-mediated Ambn expression, and contribute to dental epithelial cell differentiation into ameloblasts. (elsevier.com)
- These results support the notion that the dentin proteins expressed by presecretory ameloblasts contribute to the unique properties of the dentino-enamel junction. (nih.gov)
- Laser capture micro-dissection will be used to separately collect presecretory, secretory and maturation stage ameloblasts from mice with and without fluoride exposure for either whole transcript comparisons, or qPCR analysis of genes that modulate pH in the developing enamel matrix. (grantome.com)
- current work in includes a comparative genomic study of genes that code for enamel matrix proteins and associated proteases to document whether the thick enamel trait in humans is associated with positive selection in the noncoding regions of these genes. (duke.edu)
- This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. (technologynetworks.com)
- Mice lacking expression of the AmelX (show AMELX ELISA Kits ), Enam (show ENAM ELISA Kits ) and Mmp20 (show MMP20 ELISA Kits ) genes have been generated. (antibodies-online.com)
- Because of this, we speculated that dental pulp cells would be capable of expressing dentin-specific genes and proteins inside the diffusion chamber in the host microenvironment. (scielo.org.ar)
- Pathway analyses indicated that molar ankylosis is associated with the expression of genes consistent with the cellular inflammatory response and epithelial cell turnover. (bvsalud.org)
- The selected pathways were used to design panels of real-time PCR primers tailored for the top-ranked genes for differential gene expression analysis. (bio-rad.com)
- Reference genes are used in relative gene expression analysis to normalize for variation in the amount of input messenger RNA (mRNA) among samples. (bio-rad.com)
- To ensure accurate quantitation, it is important to include one or more reference genes exhibiting constant expression levels under the experimental conditions. (bio-rad.com)
- Epithelial at first, these signals induce their own expression and expression of specific genes in mesenchymal cells that themselves induce the morphogenesis of the tooth bud. (biomedcentral.com)
- Exposure of enamel cells to fluoride resulted in decreases in ER Ca 2+ content and store-operated Ca 2+ entry into the ER, reduced the expression of genes encoding ER stress-response proteins, and resulted in mitochondrial dysfunction. (sciencemag.org)
- Comparison of all known mouse Dact genes, Dact1-3, from the morphological initiation of mandibular first molar development after the onset of the root formation using sectional in situ hybridization showed distinct, complementary and overlapping expression patterns for the studied genes. (pubmedcentralcanada.ca)
- To identify Yap target genes that are activated during cardiomyocyte renewal and regeneration, we performed Yap chromatin immunoprecipitation sequencing (ChIP-Seq) and mRNA expression profiling in Hippo signaling¡Vdeficient mouse hearts. (phalanx.com.tw)
- We found that Yap directly regulated genes encoding cell cycle progression proteins, as well as genes encoding proteins that promote F-actin polymerization and that link the actin cytoskeleton to the extracellular matrix. (phalanx.com.tw)
- Our data showed that, in addition to genes encoding cell cycle progression proteins, Yap regulated genes that enhance cytoskeletal remodeling. (phalanx.com.tw)
- The genes themselves are only important in that they encode the proteins,?Lamont said. (bio-medicine.org)
- Notably, the top 15 SMARCAL1 -interacting genes all showed a similar renal temporal expression pattern. (frontiersin.org)
- ER stress genes associated with the unfolded protein response were increased in Stim1/2 -deficient cells, whereas the expression of components of the glutathione system were decreased. (jci.org)
- The term "proteomics" is a blend of "protein" and "genome" [ 5 ] was first coined in the year 1997 by James to make an analogy with genomics, the study of the genes [ 6 ]. (hindawi.com)
- The levels of mRNA expression of target genes regulated, directly or indirectly, by the 214/199 cluster were measured using deoxyoligonucleotide microarrays. (uio.no)
- Transcription factors are proteins that interact with DNA to turn genes on or off. (eurekalert.org)
- Protein expression of the early response genes, jun and fos, has been suggested to play an important role in the in vitro and in vivo proliferation of adrenal cells. (biomedsearch.com)
- The transcription levels of these genes were all elevated in the presence of L-ascorbate, and the expression of ptxA gene decreased significantly once ptxB gene was knockout. (biomedcentral.com)
- To determine the effects of fluoride on the expression of genes related to enamel matrix protein deposition and pH regulation. (grantome.com)
- C6orf58 (Chromosome 6 Open Reading Frame 58) is a Protein Coding gene. (genecards.org)
- The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. (genecards.org)
- FGF3 (Fibroblast Growth Factor 3) is a Protein Coding gene. (genecards.org)
- ddPCR ™ Evagreen assay for gene expression analysis. (bio-rad.com)
- A mutation in this gene which alters the normal splice pattern and results in premature termination of the encoded protein has been associated with amelogenesis imperfecta. (bio-rad.com)
- The extracellular forms of latent TGF-ß are also associated with a second gene product, the latent TGF-ß binding protein (LTBP). (nyu.edu)
- Method: The gene expression was analyzed using several mouse tissues by real time PCR. (umich.edu)
- Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). (scielo.org.ar)
- In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro . (scielo.org.ar)
- Thus, to clarify the influence of the microenvironment in protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1cells (bone marrow stromal cell line) were seeded either in cell culture or in intra-abdominal diffusion chambers. (scielo.org.ar)
- In humans, the Tuftelin protein is encoded by the TUFT1 gene. (wikipedia.org)
- Hierarchical clustering and principal component analysis showed clear separation in gene expression between infraoccluded and non-infraoccluded samples. (bvsalud.org)
- Bio-Rad collaborated with Biogazelle, leaders in real-time PCR research, to design and experimentally validate PCR primers for gene expression assays across the human and mouse transcriptomes. (bio-rad.com)
- Each gene target within a pathway was assigned a score based on the frequency of differential expression and its research significance. (bio-rad.com)
- During tooth development, BMPs gene transcription appears to be involved in early condensation and further differentiation of the dental mesenchyme and of its derivatives. (go.jp)
- Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. (abcam.com)
- Because of their similar structural and developmental features, gene expression patterns were described over the four developmental stages of both tooth and scale buds in the catshark. (biomedcentral.com)
- These gene expression patterns differ from mouse tooth development, and discrepancies are also observed between tooth and scale development within the catshark. (biomedcentral.com)
- Similarities in the topology of gene expression pattern, including Bmp signaling pathway, suggest that mouse molar development is more similar to scale bud development in the catshark. (biomedcentral.com)
- Gene overexpression and silencing experiments were used to up- and down-regulate Foxo1 expression. (springer.com)
- In this study, we examined for the first time the localization of both BGP and MGP gene expression and protein accumulation within the different regions of the vertebrate tooth. (ualg.pt)
- We clearly demonstrated that although the overall pattern of BGP and MGP gene expression and protein accumulation in A. regius teeth was in general agreement to what is known for other vertebrates such as rats or rodents, our study provided novel information and highlighted some species-differences between fish and higher vertebrates. (ualg.pt)
- To investigate the role of Cav3.2 in global gene expression, we performed a microarray transcriptome study on the hippocampi of the Cav3.2-/- mice and their wild-type littermates, either naïve (untrained) or trace fear conditioned. (phalanx.com.tw)
- By contrast, overexpression of Dpysl4 in dental epithelial cells induces inhibition of growth and increases the expression of the inner enamel epithelial cell marker gene, Msx2 . (ijbs.com)
- It has been reported that the formation of epithelial polarity is regulated by several signaling mechanisms including Phosphatidylinositol 3,4,5-trisphosphate (PIP 3 ) kinase, Glycogen synthase kinase 3 beta (GSK3β), Partitioning-defective protein (PAR) complex and the Ras homolog gene family (Rho) of proteins through the rearrangement of the actin cytoskeleton and microtubules [ 9 ]. (ijbs.com)
- Publications] Nagatsuka, H.: 'Gene expression of chondro-osseous cells in heterotopic bone formation induced by rhBMP-2'J. (nii.ac.jp)
- Deciphering the structure of the human tuftelin gene, mRNA and protein, and its function during normoxia, hypoxia, cancer progression and mouse embryonic craniofacial development. (yissum.co.il)
- Furthermore, they found that the Sp7 transcription factor directly controlled gene expression of the Fgf ligands. (eurekalert.org)
- The contribution of epigenetic modifications of the ZTRE to age-associated changes in the regulation of zinc transporter gene expression. (ncl.ac.uk)
- Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. (bionity.com)
- We have analysed the expression, regulation and function of the jagged 2 (Jag2) gene, which encodes a ligand for the Notch family of receptors, in developing mouse teeth. (jax.org)
- In that way, its inhibitory function on the transcription factor AP-1, indispensable to maintain human papillomavirus (HPV) gene expression is relieved. (biomedsearch.com)
- Thyroid hormone-induced calorigenesis triggers liver oxidative stress with concomitant TNF-alpha production by Kupffer cells and up-regulation of gene expression. (biomedsearch.com)
- The gene for dentin sialophosphoprotein produces a single protein that is post-translationally modified to generate two distinct extracellular proteins: dentin sialoprotein and dentin phosphoprotein. (nih.gov)
- Furthermore, we propose that when matrix proteins or mineralizing apatite are not available to bind fluoride ions (ie presecretory stage), fluoride can have a direct effect on gene expression. (grantome.com)
- We have shown that the mechanisms responsible for enamel fluorosis include both fluoride related effects in the developing enamel matrix, and altered gene expression. (grantome.com)
- In this proposal we further explore the mechanisms of fluorosis, by analyzing changes in matrix protein hydrolysis, ameloblast modulation and stage specific gene expression in rodent incisor models for enamel fluorosis. (grantome.com)
- The association between caries experience/activity and the electrophoretic profiles of salivary proteins lactoferrin and lysozyme was evaluated. (bvsalud.org)
- A clinical examination to diagnose the presence or absence of dental caries, by means of the DMFT index, was conducted. (bvsalud.org)
- METHODS: The study was a comparative analytical study involving the placement of 25 SSC restorations using the conventional method and 25 SSC restorations using the Hall technique in 25 subjects aged 3-8 years with a pair or pairs of unrestored enamel or dentinal carious primary molars matched for tooth type, dental arch and extent of caries. (bvsalud.org)
- The Hall Technique appears to be a good method for managing dental caries in primary molar teeth especially in a resource challenged environment where electricity and access to care are contending issues. (bvsalud.org)
- Health USDo, Human Services Federal Panel on Community Water F (2015) U.S. Public Health Service recommendation for fluoride concentration in drinking water for the prevention of dental caries. (springer.com)
- In contrast, excessive fluoride intake causes dental fluorosis, visually recognizable enamel defects that can increase the risk of caries. (sciencemag.org)
- Dental caries is a largely preventable condition and fluoride has proven an effective caries prophylactic. (frontiersin.org)
- The U.S. Public Health Service (PHS) recommends public water fluoridation at an optimal fluoride concentration of 0.7 ppm (corresponding to 0.04 mM NaF) in order to prevent dental caries ( Health and Human Services Federal Panel on Community Water, 2015 ). (frontiersin.org)
- Tooth deterioration generally occurs due to bacterial or environmental factors, where the main bacteria-related tooth conditions are dental plaque and caries. (lifemapsc.com)
- In the United States, dental caries is the most common chronic childhood disease. (lifemapsc.com)
- Despite the involvement and importance of fluoride in reducing dental caries incidence, the mechanism of fluoride absorption and metabolism remain unclear. (ncl.ac.uk)
- Although in general Thio-GLU was not significantly better than GLU alone in stimulating salivary IgA responses and in protection against dental caries, the finding that the GLU polypeptide alone, in the absence of any immunoenhancing agents, is protective against disease offers a promising and safe strategy for the development of a vaccine against caries. (asm.org)
- An important application of studying the molecular pathogenesis of dental caries and identifying virulence factors of S. mutans is to develop a mucosal subunit vaccine which would inhibit these factors by inducing substantial levels of secretory immunoglobulin A (IgA) antibodies in saliva. (asm.org)
- The feasibility of this objective has been demonstrated by oral immunization experiments with GTF in animal models of dental caries ( 26 ). (asm.org)
- Szkaradkiewicz-Karpińska AK, Ronij A, Goślińska-Kuźniarek O, Przybyłek I, Szkaradkiewicz A. MUC7 Level As A New Saliva Risk Factor For Dental Caries In Adult Patients. (medsci.org)
- Our study aimed to determine the relationship between dental caries in adults and levels of MUC5B and MUC7. (medsci.org)
- Analysis of the obtained data receiver operating characteristic (ROC) curves was employed to define relationships between the contents of the studied mucins and the detected dental caries. (medsci.org)
- In parallel, a relationship was detected between levels of the examined mucins and manifestation of dental caries. (medsci.org)
- For MUC7, the optimal cut-off value was obtained (i.e. corresponding to 100% sensitivity and specificity), amounting to 2.5 ng/ml for the detection of dental caries risk. (medsci.org)
- Development of dental caries is linked to reduced concentrations of MUC5B and MUC7. (medsci.org)
- The etiopathogenesis of dental caries is complex, with several causes, and has not been completely clarified [ 1 , 2 ]. (medsci.org)
- Currently, the principal etiological agent initiating dental caries is known to involve cariogenic bacteria, oral streptococci (mostly Streptococcus mutans ), early stage carious lesions and lactobacilli from advanced lesions [ 1 , 3 , 4 ]. (medsci.org)
- In our previous studies, we demonstrated a significant association between salivary acidic proline-rich proteins (APRP-1/2) and the severity of caries [ 8 ]. (medsci.org)
- Several data suggest the potential significance of salivary mucins in protection against dental caries [ 12 , 13 , 14 , 15 ]. (medsci.org)
- Nevertheless, it remains to be established whether the manifestation of dental caries in adults is associated with quantitative disturbances in the above glycoproteins. (medsci.org)
- This study aimed at determining the relationship between dental caries in adults and levels of MUC5B and MUC7. (medsci.org)
- Dental pulp cells (DPCs), including dental pulp (DP) stem cells, play a role in dentine repair under certain conditions caused by bacterial infections associated with caries, tooth fracture and injury. (spandidos-publications.com)
- These imperfections in enamel subject the whole tooth to wear or caries attack, typically leading to subsequent failure of the tooth and decreased quality of life. (nih.gov)
- Amaechi BT, Higham SM, Edgar WM (1999a) Effect of sterilization methods on the structural integrity of artificial enamel caries for intra-oral cariogenicity tests. (springermedizin.de)
- Streptococcus mutans is the primary etiological agent of human dental caries. (biomedcentral.com)
- The mechanisms of transport and metabolism of carbohydrates by S. mutans are therefore crucial to the onset and development of dental caries. (biomedcentral.com)
- A thorough understanding of the mechanisms by which fluoride can alter enamel formation will allow us to develop strategies to minimize the negative effects of fluoride while enhancing the use of fluoride for caries prevention We will investigate these potential mechanisms of dental fluorosis through the following three specific aims. (grantome.com)
- Objective: Patients with Amelogenesis Imperfecta (AI) caused by FAM20A mutation display several dental phenotypes including hypoplastic enamel, intrapulpal calcification, delayed tooth eruption, failure of tooth development and gingival hyperplasia. (umich.edu)
- E-cadherin was detected in every layer of the enamel organ during the different stages of tooth development, but there were slight differences between first-generation and replacement teeth in the strength and distribution of the signal. (biomedcentral.com)
- This review aims to survey the hitherto data on BMPs during tooth development with an attempt to stress a possible functional relationship between epithelial BMPs expression and regressive processes representing integral part of normal odontogenesis. (go.jp)
- In this study, the tissue distribution and accumulation of osteocalcin or bone Gla protein (BGP) and matrix Gla protein (MGP) were determined during tooth development in a teleost fish, Argyrosomus regius. (ualg.pt)
- SLC24A4 encodes a calcium transporter that mediates calcium transport to developing enamel during tooth development . (wikipedia.org)
- Differential diagnosis would include dental fluorosis , molar-incisor hypomineralization , chronological disorders of tooth development. (wikipedia.org)
- BIRMINGHAM, Ala. - Amjad Javed, Ph.D., and University of Alabama at Birmingham colleagues have found a key role in tooth development for the transcription factor Specificity protein 7, or Sp7. (eurekalert.org)
- PMID30584670}} 'Dental follicle cells (DFCs) activate and recruit osteoclasts for tooth development and tooth eruption, whereas DFCs themselves differentiate into osteoblasts to form alveolar bone surrounding tooth roots through the interaction with Hertwig's epithelial root sheath (HERS). (edu.au)
- In normal incisors, localization patterns of the basement membrane (stained for collagen IV and laminin β1/γ1 [subunits of laminin 10/11, a major laminin in early tooth development]) and enamel matrix (stained for ameloblastin) were reciprocal (Fig. 6). (nih.gov)
- However, the expression patterns and functions of GSLs during tooth development remain unclear. (elsevier.com)
- Although these two factors are differentially expressed in many tissues, they show overlapping expression during tooth development. (biologists.org)
- Here, we report the creation of ameloblastin- mice, which developed severe enamel hypoplasia. (nih.gov)
- Exogenous GM3 and LacCer in dental epithelial cells induced the expression of ameloblastin (Ambn), while it was also interesting that GM3 synergistically exerted enhancement of NT-4-mediated Ambn expression. (elsevier.com)
- the enamel knot, iii. (go.jp)
- However, a similar nested expression of Shh and Fgf suggests similar signaling involved in morphogenesis of all structures, although apoptosis assays do not support a strictly equivalent enamel knot system in sharks. (biomedcentral.com)
- These results support the fact that no enamel knot, as described in mammalian teeth, can be described in the morphogenesis of shark teeth or scales. (biomedcentral.com)
- Collectively these results suggest that Dact1 and -3 may contribute to early tooth formation by modulation of Wnt signaling pathways in the mesenchyme, including preodontoblasts, whereas Dact2 may play important signal-modulating roles in the adjacent epithelial cells including the enamel knot signaling centers and preameloblasts. (pubmedcentralcanada.ca)
- Dental bud cells differentiate and give rise to the main epithelial signaling center known as enamel knot . (lifemapsc.com)
- This was due to alterations in the enamel knot, an epithelial signalling structure involved in molar crown morphogenesis, in which Bmp4 expression and apoptosis were altered. (jax.org)
- Fgf8 and Bmp4 expression patterns are disturbed and the enamel knot fails to develop. (biologists.org)
- The effect of fluoride on expression of Foxo1, kallikrein-4 (Klk4), and amelotin (Amtn) mRNAs was analyzed by real-time RT-PCR, and western blotting was used to measure total and nuclear FOXO1 protein levels in mature dental epithelial cells. (fluoridealert.org)
- In addition to the reduced levels of Foxo1, Klk4, and AmtnmRNAs, the protein levels of total and nuclearFOXO1 were decreased in the mature dental epithelial cells exposed to fluoride. (fluoridealert.org)
- The underlying mesenchyme condensates and at the same time, epithelial cells continue proliferating to form the dental bud. (lifemapsc.com)
- Although C. difficile toxin A is known to induce nuclear factor-kappaB-mediated chemokine expression in intestinal epithelial cells, little is known about its effect on the regulation of activator protein-1 (AP-1) by mitogen-activated protein kinase (MAPK). (biomedsearch.com)
- In addition, consistently exogenous GM3 and LacCer in dental epithelial cells induced distinct activation of extracellular signal-regulated kinase 1/2 (ERK1/2), an event upstream of the expression of Ambn. (elsevier.com)
- Furthermore, depletion of GSLs from dental epithelial cells by D-threo-1-phenyl-2-decanoylamino-3- morpholino-1-propanol (D-PDMP) inhibited Ambn expression as well as phosphorylation of ERK1/2. (elsevier.com)
- Mechanisms of regeneration of tooth supporting tissues (alveolar bone, cementum and periodontal ligament) using recombinant proteins and engineered mesenchymal stem cells. (yissum.co.il)
- Here we present the construction and characterization of a chimeric vaccine protein combining the glucan-binding domain (GLU) of the gtfB -encoded water-insoluble glucan-synthesizing glucosyltransferase enzyme (GTF-I) from Streptococcus mutans and thioredoxin from Escherichia coli , which increases the solubility of coexpressed recombinant proteins and stimulates proliferation of murine T cells. (asm.org)
- Enamel deposition on top of enameloid. (biomedcentral.com)
- F: Early deposition of enamel matrix. (biomedcentral.com)
- Enamel matrix deposition. (biomedcentral.com)
- now show that Fam20A binds to Fam20C, and this increases the ability of Fam20C to phosphorylate the proteins that form the 'matrix' that guides the deposition of the enamel minerals. (elifesciences.org)
- Both KLK4 and AMTN proved to be essential for the maturation of dental enamel, a process that requires the removal of extracellularmatrix proteins and the deposition of ions on the sides of enamel crystallites. (antibodies-online.com)
- In incisors, cytodifferentiation and enamel matrix deposition were inhibited. (jax.org)
- To determine how fluoride-related changes in enamel matrix pH affects ameloblast modulation and mineral deposition. (grantome.com)
- The breakage of enamel near the dentino-enamel junction in Klk4 -null mice is not due to a failure of odontoblasts to express Klk4 , but it relates to a progressive hypomineralization of enamel with depth. (antibodies-online.com)
- Enamel fluorosis is characterized by hypomineralization, and forkhead box O1 (Foxo1) is essential for mouse enamel biomineralization. (fluoridealert.org)
- To characterize the biological function of FAM20A, as a preliminary step, the present study is sought to investigate the expression and localization of endogenous Fam20a in an ameloblast cell line, LS8. (umich.edu)
- The expression was also confirmed in the LS8 ameloblast cell line. (umich.edu)
- So far, cell-based enamel tissue engineering has been hampered by the limitations of presently existing ameloblast cell lines. (bvsalud.org)
- These pH-related effects alter ameloblast function and modulation, and enamel maturation. (grantome.com)
- AMELX and ENAM encode extracellular matrix proteins of the developing tooth enamel and KLK-4 and MMP20 encode proteases that help degrade organic matter from the enamel matrix during the maturation stage of amelogenesis . (wikipedia.org)
- Rats treated with fluoride (0, 50, 100 and 125ppm) in drinking water for 6weeks had significantly elevated expression levels of Sirt1, Atg5, Atg7 and Atg8/LC3 in their maturation stage enamel organs. (elsevier.com)
- Intriguingly, \(F^−\)-treated rats demonstrated a striking decrease in transcripts expressed during the maturation stage of enamel development (Klk4 and Amtn). (harvard.edu)
- Dipeptidyl peptidase I (show CTSC ELISA Kits ) activates pro- KLK4 to cleave a fluorogenic peptide containing a KLK4 cleavage site in mice incisor enamel organs during amelogenesis. (antibodies-online.com)
- Enamel in Stim1/2 K14cre mice was hypomineralized with decreased Ca content, mechanically weak, and thinner. (jci.org)
- Mice homozygous for a deletion in the Notch-interaction domain of Jag2 presented a variety of severe dental abnormalities. (jax.org)
- Mechanistically, Fam20A is a pseudokinase that forms a functional complex with Fam20C, and this complex enhances extracellular protein phosphorylation within the secretory pathway. (elifesciences.org)
- Reversible phosphorylation is a fundamental mechanism used to regulate cellular signaling and protein function. (elifesciences.org)
- Bone Morphogenetic Proteins (BMPs) exhibit multiple functions during development. (go.jp)
- With few exceptions, enamel microhardness, fluorosis levels, and fluoride concentrations of bone and urine did not differ significantly between fluoride treated animals fed with control-chow or 4PBA-chow. (frontiersin.org)
- The elucidation of action mechanism of enamel matrix derivative on the reconstruction of alveolar bone. (nii.ac.jp)
- The proteome map, that is, complete catalogue of the matrix and cellular proteins expressed in alveolar bone, cementum, periodontal ligament, and gingiva, is to be explored for more in-depth understanding of periodontium. (hindawi.com)
- Proteome analysis of bone and dental structure (enamel, periodontal ligament, and cementum) and oral fluid diagnostics (saliva and GCF) are the primary areas where dental proteomics has shown promising outcomes [ 7 ]. (hindawi.com)
- Have been working on different aspects of adipokines, expression and effects, since 1996, and was instrumental in identifying the expression of adipokines (leptin, adiponectin and resitin) in bone. (uio.no)
- In dental explants cultured in vitro, the local application of fibroblast growth factors upregulated Jag2 expression, whereas bone morphogenetic proteins provoked the opposite effect. (jax.org)
- Distal cis-regulatory elements are required for tissue-specific expression of enamelin (Enam). (ozgene.com)
- The aim of this study was to characterize the cis-regulatory sequences necessary for normal expression of enamelin (Enam). (ozgene.com)
- Only the 5.2-Enam-LacZ construct was sufficient to recapitulate the endogenous pattern of enamelin tooth-specific expression. (ozgene.com)
- Potential transcription factor-binding sites were identified that may be important in controlling enamelin basal promoter activity and in conferring enamelin tissue-specific expression. (ozgene.com)
- Our study provides new insights into regulatory mechanisms governing enamelin expression. (ozgene.com)
- The application of an enamel matrix protein derivative (Emdogain) in regenerative periodontal therapy: A review. (wikipedia.org)
- The National Institute of Dental and Craniofacial Research estimates 80 percent of adult Americans have some form of periodontal disease, their symptoms ranging from mild gum irritation to complete tooth loss. (bio-medicine.org)
- A customized dental prosthesis for periodontal or osseointegration is disclosed having a manufactured implant portion shaped to substantially conform to the three-dimensional surface of a root of a tooth to be replaced. (google.com.au)
- Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development reproduction and tissue remodeling as well as in disease processes such as arthritis and metastasis. (bio-rad.com)
- Dental enamel is the hardest tissue of the body. (cuni.cz)
- Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions. (abcam.com)
- Tooth enamel is a complex mineralized tissue consisting of long and parallel apatite crystals configured into decussating enamel rods. (bvsalud.org)
- In the present review, we discuss five pathways toward enamel tissue engineering, (i) enamel synthesis using physico-chemical means, (ii) protein matrix-guided enamel crystal growth, (iii) enamel surface remineralization, (iv) cell-based enamel engineering, and (v) biological enamel regeneration based on de novo induction of tooth morphogenesis. (bvsalud.org)
- Tooth enamel is the hardest tissue in the body. (bvsalud.org)
- Role of Zinc transporters in dental pulp tissue. (ncl.ac.uk)
- It is formed by an evolutionarily highly conserved biomineralization process that is controlled by proteins of extracellular matrix. (cuni.cz)
- In addition, we hypothesize that the expression of alpha-2-HS-glycoprotein, which has not been previously observed in enamel proteomes, had a role in the biomineralization of the thick enamel crowns that characterize the large molars in Gigantopithecus 9 , 10 . (nature.com)
- Fam20C is the physiological Golgi casein kinase, which phosphorylates many secreted proteins and is critical for proper biomineralization. (elifesciences.org)
- Its function continues unclarified, although it plays a role in the biomineralization of the dental organ. (bvsalud.org)
- The amelogenins comprise 90% of the developing extracellular enamel matrix proteins and play a major role in the biomineralization and structural organization of enamel. (uzh.ch)
- These findings suggest that the decrease of Foxo1 expression induced by high fluoride was a cause for low KLK4 expression. (springer.com)
- Denbesten P, Li W (2011) Chronic fluoride toxicity: dental fluorosis. (springer.com)
- Suzuki M, Shin M, Simmer JP, Bartlett JD (2014) Fluoride affects enamel protein content via TGF-beta1-mediated KLK4 inhibition. (springer.com)
- Wei W, Gao Y, Wang C, Zhao L, Sun D (2013) Excessive fluoride induces endoplasmic reticulum stress and interferes enamel proteinases secretion. (springer.com)
- DenBesten PK, Yan Y, Featherstone JD, Hilton JF, Smith CE, Li W (2002) Effects of fluoride on rat dental enamel matrix proteinases. (springer.com)
- Chronic fluoride over-exposure during pre-eruptive enamel development can cause dental fluorosis. (frontiersin.org)
- In vivo , we measured fluorosis levels, enamel hardness and fluoride concentration. (frontiersin.org)
- Although 4PBA mitigated high-dose fluoride toxicity in vitro , a diet rich in 4PBA did not attenuate dental fluorosis in rodents. (frontiersin.org)
- Atg5, Atg7 and Atg8/LC3 and showed that both their transcript and protein levels were significantly increased following fluoride treatment. (elsevier.com)
- However, several recent studies in industrialised and developing countries have shown an increase in the prevalence of dental fluorosis, a condition which causes mottling of the enamel, in both water fluoridated and non-fluoridated communities, which may suggest that the threshold of fluoride exposure for maximising oral health benefit while minimising potential risk of dental fluorosis has been exceeded. (ncl.ac.uk)
- Enamel fluorosis is a defect in enamel development that occurs after exposure to excess fluoride. (grantome.com)
- In this competing renewal, we propose to build on work that we have completed in previous grant cycles, to test a new paradigm that defines fluorosis as resulting from matrix related changes that occur as fluoride is incorporated into the forming enamel mineral. (grantome.com)
- These studies will be done with an exceptional team of international investigators, and we anticipate that at the completion of this grant cycle, that we will have a thorough understanding of how fluoride exposure during enamel development results in fluorosis. (grantome.com)
- In this work, we describe expression patterns and proliferation/apoptosis assays to uncover homologous regulatory pathways in the catshark Scyliorhinus canicula . (biomedcentral.com)
- In this study, comparative proteomic analysis was conducted to investigate the roles of specific proteins in the pathways regarding keratinocyte proliferation in cholesteatoma. (mdpi.com)
- The results of this study suggested that phosphorylated HSP27 is the end expression of two potential signal-transduction pathways, and together with PRDX2, they are very likely involved in the proliferation of keratinocytes in cholesteatoma. (mdpi.com)
- Amaechi BT, Higham SM, Edgar WM (1998) Efficacy of sterilization methods and their effect on enamel demineralization. (springermedizin.de)
- It can metabolize a wide variety of carbohydrates and produce large amounts of organic acids that cause enamel demineralization. (biomedcentral.com)
- These metabolic byproducts cause a substantial drop in the pH of the oral cavity that in turn can result in the demineralization of enamel. (biomedcentral.com)
- The localization of FAM20 proteins were re-examined and reported that the immunoreactivity of FAM20A was not clearly observed in the enamel matrix. (umich.edu)
- The subcellular localization of endogenous Fam20a protein was detected at the cytoplasm. (umich.edu)
- With an interest in compartmentation as a way of controlling glycosyltransferase activity, as well as a general interest in how proteins are localized in the Golgi, several investigators have been studying the signals and mechanisms that direct glycosyltransferase Golgi localization. (go.jp)
- The first of these is the bilayer thickness mechanism of Golgi retention and is based on the fact that the transmembrane domains of many Golgi proteins play important roles in their localization. (go.jp)
- Using indirect immunofluorescence on cryosections, we characterized the changes in protein localization of α1 to α6 chains of type IV collagen during mouse molar development. (nii.ac.jp)
- RT-PCR results after In vivo transfection with antagomir-214 suggest that temporarily knockout of miR-214 induces opposite responses on the levels of expression of miR-199-5p and miR-199-3p. (uio.no)
- Stromal cell-derived factor-1 induces matrix metalloprotease-13 expression in human chondrocytes. (biomedsearch.com)