Proteins obtained from ESCHERICHIA COLI.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Proteins found in any species of bacterium.
Infections with bacteria of the species ESCHERICHIA COLI.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The functional hereditary units of BACTERIA.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A negative regulator of beta-catenin signaling which is mutant in ADENOMATOUS POLYPOSIS COLI and GARDNER SYNDROME.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection.
The rate dynamics in chemical or physical systems.
A species of gram-negative, rod-shaped bacteria belonging to the K serogroup of ESCHERICHIA COLI. It lives as a harmless inhabitant of the human LARGE INTESTINE and is widely used in medical and GENETIC RESEARCH.
Proteins isolated from the outer membrane of Gram-negative bacteria.
Proteins prepared by recombinant DNA technology.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
The sum of the weight of all the atoms in a molecule.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Viruses whose host is Escherichia coli.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Strains of ESCHERICHIA COLI that produce or contain at least one member of either heat-labile or heat-stable ENTEROTOXINS. The organisms colonize the mucosal surface of the small intestine and elaborate their enterotoxins causing DIARRHEA. They are mainly associated with tropical and developing countries and affect susceptible travelers to those places.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Strains of ESCHERICHIA COLI with the ability to produce at least one or more of at least two antigenically distinct, usually bacteriophage-mediated cytotoxins: SHIGA TOXIN 1 and SHIGA TOXIN 2. These bacteria can cause severe disease in humans including bloody DIARRHEA and HEMOLYTIC UREMIC SYNDROME.
Substances that reduce the growth or reproduction of BACTERIA.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Strains of ESCHERICHIA COLI characterized by attaching-and-effacing histopathology. These strains of bacteria intimately adhere to the epithelial cell membrane and show effacement of microvilli. In developed countries they are associated with INFANTILE DIARRHEA and infantile GASTROENTERITIS and, in contrast to ETEC strains, do not produce ENDOTOXINS.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Transport proteins that carry specific substances in the blood or across cell membranes.
Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria whose organisms occur in the lower part of the intestine of warm-blooded animals. The species are either nonpathogenic or opportunistic pathogens.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Strains of ESCHERICHIA COLI that are a subgroup of SHIGA-TOXIGENIC ESCHERICHIA COLI. They cause non-bloody and bloody DIARRHEA; HEMOLYTIC UREMIC SYNDROME; and hemorrhagic COLITIS. An important member of this subgroup is ESCHERICHIA COLI O157-H7.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Strains of Escherichia coli that preferentially grow and persist within the urinary tract. They exhibit certain virulence factors and strategies that cause urinary tract infections.
Periplasmic proteins that bind MALTOSE and maltodextrin. They take part in the maltose transport system of BACTERIA.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Bacterial proteins that are used by BACTERIOPHAGES to incorporate their DNA into the DNA of the "host" bacteria. They are DNA-binding proteins that function in genetic recombination as well as in transcriptional and translational regulation.
Any method used for determining the location of and relative distances between genes on a chromosome.
Vaccines or candidate vaccines used to prevent or treat both enterotoxigenic and enteropathogenic Escherichia coli infections.
An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.
Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
The process by which a DNA molecule is duplicated.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A subdiscipline of genetics which deals with the genetic mechanisms and processes of microorganisms.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It is closely related to SHIGA TOXIN produced by SHIGELLA DYSENTERIAE.
A species of gram-positive bacteria that is a common soil and water saprophyte.
A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
Inflammatory responses of the epithelium of the URINARY TRACT to microbial invasions. They are often bacterial infections with associated BACTERIURIA and PYURIA.
An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It shares 50-60% homology with SHIGA TOXIN and SHIGA TOXIN 1.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
A form of gram-negative meningitis that tends to occur in neonates, in association with anatomical abnormalities (which feature communication between the meninges and cutaneous structures) or as OPPORTUNISTIC INFECTIONS in association with IMMUNOLOGIC DEFICIENCY SYNDROMES. In premature neonates the clinical presentation may be limited to ANOREXIA; VOMITING; lethargy; or respiratory distress. Full-term infants may have as additional features FEVER; SEIZURES; and bulging of the anterior fontanelle. (From Menkes, Textbook of Child Neurology, 5th ed, pp398-400)
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Life or metabolic reactions occurring in an environment containing oxygen.
A group I chaperonin protein that forms a lid-like structure which encloses the non-polar cavity of the chaperonin complex. The protein was originally studied in BACTERIA where it is commonly referred to as GroES protein.
Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments.
A class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS. They include SHIGA TOXIN which is produced by SHIGELLA DYSENTERIAE and a variety of shiga-like toxins that are produced by pathologic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A group I chaperonin protein that forms the barrel-like structure of the chaperonin complex. It is an oligomeric protein with a distinctive structure of fourteen subunits, arranged in two rings of seven subunits each. The protein was originally studied in BACTERIA where it is commonly referred to as GroEL protein.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
Proteins found in the PERIPLASM of organisms with cell walls.
The genetic complement of a BACTERIA as represented in its DNA.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
A class of plasmids that transfer antibiotic resistance from one bacterium to another by conjugation.
A non-metabolizable galactose analog that induces expression of the LAC OPERON.
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Substances elaborated by bacteria that have antigenic activity.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The space between the inner and outer membranes of a cell that is shared with the cell wall.
A synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. It is an inhibitor of the A subunit of bacterial DNA GYRASE.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
The process of cleaving a chemical compound by the addition of a molecule of water.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
Protein factors uniquely required during the elongation phase of protein synthesis.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.
The lipopolysaccharide-protein somatic antigens, usually from gram-negative bacteria, important in the serological classification of enteric bacilli. The O-specific chains determine the specificity of the O antigens of a given serotype. O antigens are the immunodominant part of the lipopolysaccharide molecule in the intact bacterial cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
An essential amino acid that is required for the production of HISTAMINE.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
Cells, usually bacteria or yeast, which have partially lost their cell wall, lost their characteristic shape and become round.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Porins are protein molecules that were originally found in the outer membrane of GRAM-NEGATIVE BACTERIA and that form multi-meric channels for the passive DIFFUSION of WATER; IONS; or other small molecules. Porins are present in bacterial CELL WALLS, as well as in plant, fungal, mammalian and other vertebrate CELL MEMBRANES and MITOCHONDRIAL MEMBRANES.
A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that ferments sugar without gas production. Its organisms are intestinal pathogens of man and other primates and cause bacillary dysentery (DYSENTERY, BACILLARY).
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
A syndrome that is associated with microvascular diseases of the KIDNEY, such as RENAL CORTICAL NECROSIS. It is characterized by hemolytic anemia (ANEMIA, HEMOLYTIC); THROMBOCYTOPENIA; and ACUTE RENAL FAILURE.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
The relationships of groups of organisms as reflected by their genetic makeup.
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis.
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
A toxin produced by SHIGELLA DYSENTERIAE. It is the prototype of class of toxins that inhibit protein synthesis by blocking the interaction of ribosomal RNA; (RNA, RIBOSOMAL) with PEPTIDE ELONGATION FACTORS.
Enzymes that catalyze the breakage of a carbon-oxygen bond leading to unsaturated products via the removal of water. EC 4.2.1.
A class of enzymes that catalyze the cleavage of C-C, C-O, and C-N, and other bonds by other means than by hydrolysis or oxidation. (Enzyme Nomenclature, 1992) EC 4.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A subclass of enzymes that aminoacylate AMINO ACID-SPECIFIC TRANSFER RNA with their corresponding AMINO ACIDS.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Inflammation of the KIDNEY involving the renal parenchyma (the NEPHRONS); KIDNEY PELVIS; and KIDNEY CALICES. It is characterized by ABDOMINAL PAIN; FEVER; NAUSEA; VOMITING; and occasionally DIARRHEA.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.

A single membrane-embedded negative charge is critical for recognizing positively charged drugs by the Escherichia coli multidrug resistance protein MdfA. (1/18963)

The nature of the broad substrate specificity phenomenon, as manifested by multidrug resistance proteins, is not yet understood. In the Escherichia coli multidrug transporter, MdfA, the hydrophobicity profile and PhoA fusion analysis have so far identified only one membrane-embedded charged amino acid residue (E26). In order to determine whether this negatively charged residue may play a role in multidrug recognition, we evaluated the expression and function of MdfA constructs mutated at this position. Replacing E26 with the positively charged residue lysine abolished the multidrug resistance activity against positively charged drugs, but retained chloramphenicol efflux and resistance. In contrast, when the negative charge was preserved in a mutant with aspartate instead of E26, chloramphenicol recognition and transport were drastically inhibited; however, the mutant exhibited almost wild-type multidrug resistance activity against lipophilic cations. These results suggest that although the negative charge at position 26 is not essential for active transport, it dictates the multidrug resistance character of MdfA. We show that such a negative charge is also found in other drug resistance transporters, and its possible significance regarding multidrug resistance is discussed.  (+info)

Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation. (2/18963)

The proton motive force (PMF) renders protein translocation across the Escherichia coli membrane highly efficient, although the underlying mechanism has not been clarified. The membrane insertion and deinsertion of SecA coupled to ATP binding and hydrolysis, respectively, are thought to drive the translocation. We report here that PMF significantly decreases the level of membrane-inserted SecA. The prlA4 mutation of SecY, which causes efficient protein translocation in the absence of PMF, was found to reduce the membrane-inserted SecA irrespective of the presence or absence of PMF. The PMF-dependent decrease in the membrane-inserted SecA caused an increase in the amount of SecA released into the extra-membrane milieu, indicating that PMF deinserts SecA from the membrane. The PMF-dependent deinsertion reduced the amount of SecA required for maximal translocation activity. Neither ATP hydrolysis nor exchange with external SecA was required for the PMF-dependent deinsertion of SecA. These results indicate that the SecA deinsertion is a limiting step of protein translocation and is accelerated by PMF, efficient protein translocation thereby being caused in the presence of PMF.  (+info)

The Saccharomyces cerevisiae ETH1 gene, an inducible homolog of exonuclease III that provides resistance to DNA-damaging agents and limits spontaneous mutagenesis. (3/18963)

The recently sequenced Saccharomyces cerevisiae genome was searched for a gene with homology to the gene encoding the major human AP endonuclease, a component of the highly conserved DNA base excision repair pathway. An open reading frame was found to encode a putative protein (34% identical to the Schizosaccharomyces pombe eth1(+) [open reading frame SPBC3D6.10] gene product) with a 347-residue segment homologous to the exonuclease III family of AP endonucleases. Synthesis of mRNA from ETH1 in wild-type cells was induced sixfold relative to that in untreated cells after exposure to the alkylating agent methyl methanesulfonate (MMS). To investigate the function of ETH1, deletions of the open reading frame were made in a wild-type strain and a strain deficient in the known yeast AP endonuclease encoded by APN1. eth1 strains were not more sensitive to killing by MMS, hydrogen peroxide, or phleomycin D1, whereas apn1 strains were approximately 3-fold more sensitive to MMS and approximately 10-fold more sensitive to hydrogen peroxide than was the wild type. Double-mutant strains (apn1 eth1) were approximately 15-fold more sensitive to MMS and approximately 2- to 3-fold more sensitive to hydrogen peroxide and phleomycin D1 than were apn1 strains. Elimination of ETH1 in apn1 strains also increased spontaneous mutation rates 9- or 31-fold compared to the wild type as determined by reversion to adenine or lysine prototrophy, respectively. Transformation of apn1 eth1 cells with an expression vector containing ETH1 reversed the hypersensitivity to MMS and limited the rate of spontaneous mutagenesis. Expression of ETH1 in a dut-1 xthA3 Escherichia coli strain demonstrated that the gene product functionally complements the missing AP endonuclease activity. Thus, in apn1 cells where the major AP endonuclease activity is missing, ETH1 offers an alternate capacity for repair of spontaneous or induced damage to DNA that is normally repaired by Apn1 protein.  (+info)

Cloning and characterisation of a novel ompB operon from Vibrio cholerae 569B. (4/18963)

The ompB operon of Vibrio cholerae 569B has been cloned and fully sequenced. The operon encodes two proteins, OmpR and EnvZ, which share sequence identity with the OmpR and EnvZ proteins of a variety of other bacteria. Although the order of the ompR and envZ genes of V. cholerae is similar to that of the ompB operon of E. coli, S. typhimurium and X. nematophilus, the Vibrio operon exhibits a number of novel features. The structural organisation and features of the V. cholerae ompB operon are described.  (+info)

Role of DnaK in in vitro and in vivo expression of virulence factors of Vibrio cholerae. (5/18963)

The dnaK gene of Vibrio cholerae was cloned, sequenced, and used to construct a dnaK insertion mutant which was then used to examine the role of DnaK in expression of the major virulence factors of this important human pathogen. The central regulator of several virulence genes of V. cholerae is ToxR, a transmembrane DNA binding protein. The V. cholerae dnaK mutant grown in standard laboratory medium exhibited phenotypes characteristic of cells deficient in ToxR activity. Using Northern blot analysis and toxR transcriptional fusions, we demonstrated a reduction in expression of the toxR gene in the dnaK mutant strain together with a concomitant increase in expression of a htpG-like heat shock gene that is located immediately upstream and is divergently transcribed from toxR. This may be due to increased heat shock induction in the dnaK mutant. In vivo, however, although expression from heat shock promoters in the dnaK mutant was similar to that observed in vitro, expression of both toxR and htpG was comparable to that by the parental strain. In both strains, in vivo expression of toxR was significantly higher than that observed in vitro, but no reciprocal decrease in htpG expression was observed. These results suggest that the modulation of toxR expression in vivo may be different from that observed in vitro.  (+info)

Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes. (6/18963)

Studies of the Vibrio cholerae population, using molecular typing techniques, have shown the existence of several pathogenic clones, mainly sixth-pandemic, seventh-pandemic, and U.S. Gulf Coast clones. However, the relationship of the pathogenic clones to environmental V. cholerae isolates remains unclear. A previous study to determine the phylogeny of V. cholerae by sequencing the asd (aspartate semialdehyde dehydrogenase) gene of V. cholerae showed that the sixth-pandemic, seventh-pandemic, and U.S. Gulf Coast clones had very different asd sequences which fell into separate lineages in the V. cholerae population. As gene trees drawn from a single gene may not reflect the true topology of the population, we sequenced the mdh (malate dehydrogenase) and hlyA (hemolysin A) genes from representatives of environmental and clinical isolates of V. cholerae and found that the mdh and hlyA sequences from the three pathogenic clones were identical, except for the previously reported 11-bp deletion in hlyA in the sixth-pandemic clone. Identical sequences were obtained, despite average nucleotide differences in the mdh and hlyA genes of 1.52 and 3.25%, respectively, among all the isolates, suggesting that the three pathogenic clones are closely related. To extend these observations, segments of the recA and dnaE genes were sequenced from a selection of the pathogenic isolates, where the sequences were either identical or substantially different between the clones. The results show that the three pathogenic clones are very closely related and that there has been a high level of recombination in their evolution.  (+info)

Identification and characterization of a novel Ibe10 binding protein that contributes to Escherichia coli invasion of brain microvascular endothelial cells. (7/18963)

The molecular basis of Escherichia coli traversal of the blood-brain barrier in the development of E. coli meningitis is not well understood. We have previously shown that a novel Ibe10 protein found in cerebrospinal fluid isolates of E. coli is necessary for invasion of the brain microvascular endothelial cells (BMEC) that constitute the blood-brain barrier both in vitro and in a newborn rat model of hematogenous meningitis. Here we identified a novel Ibe10 binding molecule/receptor (Ibe10R) on both bovine BMEC (HBMEC) and human BMEC (HBMEC) that is responsible for invasion by E. coli. Ibe10R, an approximately 55-kDa protein, was purified from BBMEC by Ibe10-Ni-Sepharose affinity chromatography. Bovine Ibe10R, as well as polyclonal antibodies to Ibe10R, blocked E. coli invasion of BBMEC very effectively. The N-terminal amino acid sequence of Ibe10R showed 75% homology to serum albumin. However, the amino acid sequence of an Ibe10R fragment generated by limited enzymatic digestion did not reveal homology to any other proteins, suggesting that Ibe10R represents a novel albumin-like protein. Immunocytochemical analysis of BBMEC using anti-Ibe10R antibody suggested that only a subset of cultured BBMEC express Ibe10R on their surface. Enrichment of Ibe10R-positive BBMEC by fluorescence-activated cell sorting with anti-Ibe10R antibody resulted in enhanced invasion by E. coli. The anti-Ibe10R antibody raised against bovine Ibe10R also blocked E. coli invasion of HBMEC very effectively. Interestingly, anti-Ibe10R antibody affinity chromatography of HBMEC membrane proteins revealed a smaller protein with an approximate molecular mass of 45 kDa. These results suggest that the Ibe10 of E. coli interacts with a novel BMEC surface protein, Ibe10R, for invasion of both BBMEC and HBMEC.  (+info)

Cloning and expression of the dnaK gene of Campylobacter jejuni and antigenicity of heat shock protein 70. (8/18963)

Campylobacter jejuni is a leading cause of infectious diarrhea throughout the world. In addition, there is growing evidence that Guillain-Barre syndrome, an inflammatory demyelinating disease of the peripheral nervous system, is frequently preceded by C. jejuni infection. In the present study, the hrcA-grpE-dnaK gene cluster of C. jejuni was cloned and sequenced. The dnaK gene consists of an open reading frame of 1,869 bp and encodes a protein with a high degree of homology to other bacterial 70-kDa heat shock proteins (HSPs). The overall percentages of identity to the HSP70 proteins of Helicobacter pylori, Borrelia burgdorferi, Chlamydia trachomatis, and Bacillus subtilis were calculated to be 78.1, 60.5, 57.2, and 53. 8%, respectively. Regions similar to the Escherichia coli sigma70 promoter consensus sequence and to a cis-acting regulatory element (CIRCE) are located upstream of the hrcA gene. Following heat shock, a rapid increase of dnaK mRNA was detectable, which reached its maximum after 20 to 30 min. A 6-His-tagged recombinant DnaK protein (rCjDnaK-His) was generated in E. coli, after cloning of the dnaK coding region into pET-22b(+), and purified by affinity and gel filtration chromatography. Antibody responses to rCjDnaK-His were significantly elevated, compared to those of healthy individuals, in about one-third of the serum specimens obtained from C. jejuni enteritis patients.  (+info)

Enteropathogenic and enterohaemorrhagic Escherichia coli are related pathotypes of bacteria that cause acute watery diarrhoea and haemorrhagic colitis, respectively, and enterohaemorrhagic E. coli can lead to a serious complication known as haemolytic uraemic syndrome. In both bacteria the global regulatory protein Ler controls virulence. The ler gene is found within the locus of enterocyte effacement, or LEE, encoding a type III secretion system necessary for injecting effector proteins into intestinal epithelial cells and causing net secretory diarrhoea. The nucleoid-associated protein H-NS silences, whereas Ler serves as an anti-silencer of, multiple LEE operons. Although Ler has a higher affinity for DNA than does H-NS, the precise molecular mechanism by which Ler increases LEE transcription remains to be determined. In this report we investigate the oligomerization activity of Ler. In solution, Ler forms dimers and soluble aggregates of up to 5000 kDa molecular mass, and appears to oligomerize more
TY - JOUR. T1 - A gram-negative characteristic segment in Escherichia coli DnaK is essential for the ATP-dependent cooperative function with the co-chaperones DnaJ and GrpE. AU - Sugimoto, Shinya. AU - Higashi, Chihana. AU - Saruwatari, Kozue. AU - Nakayama, Jiro. AU - Sonomoto, Kenji. PY - 2007/6/26. Y1 - 2007/6/26. N2 - We describe importance of the characteristic segment in ATPase domain of DnaK chaperone which is present in all gram-negative bacteria but is absent in all gram-positive bacteria. In vitro studies, ATPase activity, luciferase-refolding activity, and surface plasmon resonance analyses, demonstrated that a segment-deletion mutant DnaKΔ74-96 became defective in the cooperation with the co-chaperones DnaJ and GrpE. In addition, in vivo complementation assay showed that expression of DnaKΔ74-96 could not rescue the viability of Escherichia coli ΔdnaK mutant at 43 °C. Consequently, we suggest evolutionary significance for this DnaK ATPase domain segment in gram-negative bacteria ...
In the study described here, we have taken steps to characterize the YjeE protein, an Escherichia coli protein of unknown function that is essential for bacterial viability. YjeE represents a protein family whose members are broadly conserved in bacteria, absent from eukaryotes and contain both Walker A and B motifs, characteristic of P-loop ATPases. We have revisited the dispensability of the yjeE gene in E. coli and describe efforts to probe the function of the YjeE protein with in vitro biochemistry. We have looked critically for ATPase activity in the recombinant E. coli protein and have made vigilant use of site-directed variants in the Walker A [K41A (Lys41→Ala) and T42A] and putative Walker B (D80Q) motifs. We noted that any hydrolysis of ATP by the wild-type E. coli protein might be attributed to background ATPase, since it was not appreciably different from that of the variants. To overcome potential contaminants, we turned to crystalline pure YjeE protein from Haemophilus influenzae ...
What is Escherichia coli?. Escherichia coli (abbreviated as E. coli) are a large and diverse group of bacteria. Although most strains of E. coli are harmless, others can make you sick. Some kinds of E. coli can cause diarrhea, while others cause urinary tract infections, respiratory illness and pneumonia, and other illnesses. Still other kinds of E. coli are used as markers for water contamination-so you might hear about E. coli being found in drinking water, which are not themselves harmful, but indicate the water is contaminated. It does get a bit confusing-even to microbiologists.. What are Shiga toxin-producing E. coli? Some kinds of E. coli cause disease by making a toxin called Shiga toxin. The bacteria that make these toxins are called Shiga toxin-producing E. coli, or STEC for short. You might hear them called verocytotoxic E. coli (VTEC) or enterohemorrhagic E. coli (EHEC); these all refer generally to the same group of bacteria. The most commonly identified STEC in North America is ...
AEEC - Attaching and Effacing Escherichia Coli. Looking for abbreviations of AEEC? It is Attaching and Effacing Escherichia Coli. Attaching and Effacing Escherichia Coli listed as AEEC
TY - JOUR. T1 - Use of designed metal-binding sites to study helix proximity in the lactose permease of Escherichia coli. 2. Proximity of helix IX (Arg302) with helix X (His322 and Glu325). AU - He, Molly M.. AU - Voss, John C. AU - Hubbell, Wayne L.. AU - Ronald Kaback, H.. PY - 1995. Y1 - 1995. N2 - Engineering divalent metal-binding sites into the lactose permease of Escherichia coli by introducing bis-His residues has been utilized to confirm the proximity of helices VIII (Glu269→His) and X (His322) [Jung, K., Voss, J., He, M., Hubbell, W. L., & Kaback, H. R. (1995) Biochemistry 34, 6272] and helices VII (Asp237→His) and XI (Lys358→His) [He, M. M., Voss, J., Hubbell, W. L., & Kaback, H. R. (1995) Biochemistry 34, 00000-00000]. In this paper, the approach is used to confirm and extend the relationship between helices IX (Arg302) and X (His322 and Glu325) [Jung, K., Jung, H., Wu, J., Prive, G. G., & Kaback, H. R. (1993) Biochemistry 32, 12273]. Thus, mutants Arg302→His, Glu325→His, ...
Base Sequence, Binding Sites, Catalysis, Cations; Divalent, Endoribonucleases/genetics/*metabolism, Escherichia coli/*enzymology, Escherichia coli Proteins, Hydrolysis, Molecular Sequence Data, Nucleic Acid Conformation, RNA; Catalytic/genetics/*metabolism, RNA; Messenger/chemistry/*metabolism, RNA; Transfer/chemistry/metabolism, Ribonuclease P ...
BioAssay record AID 1077160 submitted by ChEMBL: Inhibition of Escherichia coli FabH expressed in Escherichia coli BL21 (DE3) after 25 mins.
[Gastro-hemorrhagic Escherichia coli].: Groups of Escherichia coli enteropathogen are described, with special attention to Escherichia coli enterohaemorragic. S
Escherichia Coli news, clinical research studies and treatment articles dealing with e. coli infections for medical professionals to stay updated. Get our FREE app now.
BACKGROUND: Escherichia coli associated with early-onset sepsis (EOS) have historically been antibiotic-susceptible and K1-encapsulated. In the era of emerging antibiotic resistance, however, the clonal makeup of E coli associated with EOS has not been well characterized. METHODS: Escherichia coli isolates were collected from 28 cases of EOS and early-onset meningitis (EOM)
Escherichia coli ATCC ® 700927D-5™ Designation: Genomic DNA from Escherichia coli strain EDL 933 TypeStrain=False Application:
Escherichia coli ATCC ® 700927D-5™ Designation: Genomic DNA from Escherichia coli strain EDL 933 TypeStrain=False Application:
BioAssay record AID 414701 submitted by ChEMBL: Resistance index, MIC for aminoglycosides-resistant Escherichia coli BL21 harboring pETSACG1 expressing APH(3)-3a enzyme to MIC for Escherichia coli BL21.
The hns gene is a member of the cold-shock regulon, indicating that the nucleoid-associated, DNA-binding protein H-NS plays an important role in the adaptation of Escherichia coli to low temperatures. We show here that the ability to cope efficiently with a cold environment (12 degrees C and 25 degr …
This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity capture. The specificity of the polar vesicle isolation was confirmed by mass spectrometry that identified more than one hundred proteins, most of which are known inner membrane proteins, including other known polar proteins. This protocol, or if adapted properly by choosing other affinity targets, is well suited to isolate other membrane domains of interest for identification of proteins or lipid composition.
TY - JOUR. T1 - Evaluation of CTX-M steady-state mRNA, mRNA half-life and protein production in various STs of Escherichia coli. AU - Geyer, Chelsie N.. AU - Fowler, Randal C.. AU - Johnson, James R.. AU - Johnston, Brian. AU - Weissman, Scott J.. AU - Hawkey, Peter. AU - Hanson, Nancy D.. PY - 2016/3/1. Y1 - 2016/3/1. N2 - Objectives: High levels of β-lactamase production can impact treatment with a β-lactam/β-lactamase inhibitor combination. Goals of this study were to: (i) compare the mRNA and protein levels of CTX-M-15- and CTX-M-14-producing Escherichia coli from 18 different STs and 10 different phylotypes; (ii) evaluate the mRNA half-lives and establish a role for chromosomal- and/or plasmid-encoded factors; and (iii) evaluate the zones of inhibition for piperacillin/tazobactam and ceftolozane/tazobactam. Methods: Disc diffusion was used to establish zone size. RNA analysis was accomplished using real-time RT-PCR and CTX-M protein levels were evaluated by immunoblotting. Clinical ...
RseB from Escherichia coli has been crystallized and crystal structures were determined at 2.4 Å and at 2.8 Å resolution. The structure of cytoplasmic expressed RseB revealed that it consists of two domains; an N-terminal large and a C-terminal small domain. The large domain resembles an unclosed β-barrel that is structurally remarkably similar to a protein family (LolA, LolB) capable of binding the lipid anchor of lipoproteins. Detailed structural comparison of RseB and LolA led to the hypothesis that RseB might be a sensor for mislocalized lipoproteins. The small C-terminal domain, connected to the large domain by a partially unstructured loop, was identified to mediate interaction with RseA. A peptide comprised of a putative helix of RseA was shown to constitute the binding site for RseB. Structure based results presented in this thesis indicate a new role of RseB in acting as a sensor for periplasmic stress: it detects mislocalized lipoproteins in the periplasm and propagates the signal ...
Escherichia Coli or E. Coli is a bacterium commonly found in the human intestine. These bacteria consist of several types and most of them are harmless. That means that only a handful of types of E. Coli bacteria can harm health. One of the dangerous E. coli bacteria is E. coli O157: H7. These bacteria can cause food poisoning and infections that are quite serious. E. coli O157: H7 can produce poisons that can damage the walls of the small intestine and cause stomach cramps, diarrhea mixed with blood, and vomiting ...
The AcrAB system of Escherichia coli is a multidrug efflux system composed of an RND-type transporter AcrB and a periplasmic accessory protein AcrA, and pumps out a wide variety of lipophilic and amphiphilic inhibitors directly into the medium, presumably through the TolC outer membrane channel. Acr …
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You searched for: Format Text Remove constraint Format: Text Language English Remove constraint Language: English Subject RNA Remove constraint Subject: RNA Subject Escherichia coli Remove constraint Subject: Escherichia coli ...
You searched for: Format Text Remove constraint Format: Text Language English Remove constraint Language: English Subject Escherichia coli Remove constraint Subject: Escherichia coli Subject RNA Remove constraint Subject: RNA ...
The structure of the Escherichia coli chromosome is inherently dynamic over the duration of the cell cycle. Genetic loci undergo both stochastic motion around their initial positions and directed motion to opposite poles of the rod-shaped cell during segregation. We developed a quantitative method to characterize cell-cycle dynamics of the E. coli chromosome to probe the chromosomal steady-state mobility and segregation process. By tracking fluorescently labeled chromosomal loci in thousands of cells throughout the entire cell cycle, our method allows for the statistical analysis of locus position and motion, the step-size distribution for movement during segregation, and the locus drift velocity. The robust statistics of our detailed analysis of the wild-type E. coli nucleoid allow us to observe loci moving toward midcell before segregation occurs, consistent with a replication factory model. Then, as segregation initiates, we perform a detailed characterization of the average segregation velocity of
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The mechanism of transport of KP-736, a novel cephalosporin with a 1,5-dihydroxy-4-pyridone moiety at the C-7 position, into the Escherichia coli K-12 cell was investigated by determining the susceptibilities of iron transport mutants to KP-736. The tonB mutant showed a higher degree of resistance to KP-736, indicating that KP-736 was incorporated into E. coli cells via the tonB-dependent iron transport system. The product of the exbB gene was also necessary for the maximal antibacterial potency of KP-736. Cir-lacking and Fiu-lacking mutants showed a moderate level of resistance to KP-736. However, mutants lacking any one of the proteins FepA, FecA, FhuA, and FhuE did not show any increased resistance to KP-736. Two types of spontaneous mutants (e.g., KT1004 and KT1011) could be isolated from cir and fiu mutants by selection for KP-736 resistance and showed the same level of resistance to KP-736 as a tonB mutant. KT1004 showed tonB phenotypes, resistance to phage phi 80, and loss of FecA, ...
Abstract : Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinarytract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regardingantimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs)of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentionedparameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. The antimicrobialsensitivity assay was reported with 46.67% alteration (14 out of 30 tested antimicrobials) in treated group of MDR E. coli isolates.The minimum inhibitory concentration (MIC) study showed the alteration in MIC values of about 34.37% (11 out of 32) testedantimicrobials, after biofield treatment in clinical isolates of E. coli. Piperacillin/tazobactam was reported with ...
The genes (cps) involved in the synthesis of the colanic acid capsular polysaccharide in Escherichia coli K-12 are transcriptionally regulated by numerous proteins. Two of these, RcsB and RcsC, share homology with two-component regulatory elements that respond to environmental stimuli. Osmotic shock by sucrose or NaCl transiently increased transcription of a cpsB::lacZ fusion. RcsC and RcsB were essential for osmotic induction of colanic acid synthesis. In contrast to observations in some other osmotically regulated systems, addition of glycine betaine enhanced the osmotic induction of cps::lacZ by both sucrose and NaCl but had no effect alone. ...
Inhibition of disulfide bond formation in Escherichia coli implicates an intricate collaboration of proteins which comprise the glutathione and thioredoxin reducing pathways. Bioengineers have successfully engineered E. coli possessing mutated reducing pathways that promote, rather than inhibit, disulfide bond formation in the cytoplasm. The transcriptome of six such mutant E. coli strains have been characterized using Microarray technology. We find that all mutant strains, exhibit a unique response to oxidative stress, not observed in wild type. Statistical analyses revealed the expression of more than 200 genes that are affected by mutations within the reducing pathways. Significantly up-regulated biological processes include cysteine biosynthesis, histidine biosynthesis, NADH Dehydrogenase I biosynthesis, sugar catabolic processes, and activation of stress responses . The second part of this work describes the construction of an E. coli strain that promotes the complete conversion of ...
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Nucleotide sequences of translated regions of the trp operon in 12 wild strains of Escherichia coli reveal striking uniformity among eight strains (suggesting recent common ancestry and supporting the importance of periodic selection in natural populations) and clustered substitutions in four strains (implicating events affecting runs of nucleotides).
The ability of salt to rescue division in an FtsEX null mutant is enigmatic if FtsEX is needed for proper assembly or stability of the septal ring. Presumably, the downstream essential division proteins, FtsK through FtsN, all of which are required for division even on media containing salt, have some ability to localize in the absence of FtsEX. Consistent with this, RG60 is sensitive to β-lactams that target FtsI (D. Weiss, unpublished data) and we observed localization of FtsN, which is a late recruit to the division site (1), when RG60 was grown under permissive conditions. It is tempting to speculate that the ability of downstream proteins to localize independently of FtsEX is why we observed some residual localization of FtsK, FtsQ, and FtsI in filaments depleted of FtsEX in LB with no salt, but we cannot exclude the less interesting possibility that there is residual FtsEX in these filaments. Our depletion strains express ftsEX from a multicopy plasmid, so we sought to reduce the ...
Slipped-strand mispairing (SSM) has not been identified as a mechanism of phase variation in Escherichia coli. Using a reporter gene, we show that sequences that cause phase variation by SSM in Haemophilus influenzae also lead to phase variation when introduced onto the chromosome of E. coli, and the frequencies of switching are in the biologically relevant range. Thus, the absence of SSM-mediated phase variation in E. coli does not appear to be due to a mechanistic constraint. ...
The bacterium Escherichia coli is making rounds around the world. The bacteria, previously considered far less harmeless has come under in recent months. To be fair, most of the bacteria is commonly found among humans and animals alike. However, its contact with people with antibacterial resistance can lead to severe illnesses. These include cases of blood poisoning or even resulting in septic shocks. The recent breakout of these illnesses due to anti-bacterial resistance lead researchers to look a bit closer. The outbreak of E.Coli happens as some people may eat bad case of raw meat or a poor personal hygiene. If one doesnt wash his hands after visiting a restroom, they are more likely to be at risk from possible infections. This lead scientists to ask which one is more dangerous as a source of infection. Researchers from University of East Anglia in United Kingdom started this discovery on an unclear note. They were puzzled as the healthy bacteria lives in the intestine of most individuals. ...
SUMMARY: Four mutants of Escherichia coli K12 were isolated on the basis of their sensitivity to pH 5.4. Under non-permissive conditions their growth was reversibly inhibited. At pH 7.0 these mutants showed a highly pleiotropic phenotype, which included altered phage and detergent sensitivities and leakage of periplasmic proteins. The findings suggest a defect in the outer membrane, perhaps in lipopolysaccharide. Two mutants mapped in or near the rfa locus, while the other two were remote from this region.
Birkenbihl, R.P.; Vielmetter, W., 1989: Cosmid derived map of escherichia coli strain bhb2600 in comparison to the map of strain w3110
Bekijk Stockfoto van Escherichia Coli Cell With Disrupted Cell Envelope Due To Phage Release After The Phage Replicate Within Host Cells They Must Be Released From The Host Cell This Often Occurs By Lysing The Cell Sem X3500. Ga voor hoogwaardige fotos met een hoge resolutie naar Getty Images.
The Ffh-4.5S ribonucleoprotein particle (RNP) and FtsY from Escherichia coli are homologous to essential components of the mammalian signal recognition particle (SRP) and SRP receptor, respectively. The ability of these E. coli components to function in a bona fide co-translational targeting pathway remains unclear. Here we demonstrate that the Ffh-4.5S RNP and FtsY can efficiently replace their mammalian counterparts in targeting nascent secretory proteins to microsomal membranes in vitro. Targeting in the heterologous system requires a hydrophobic signal sequence, utilizes GTP and, moreover, occurs co-translationally. Unlike mammalian SRP, however, the Ffh-4.5S RNP is unable to arrest translational elongation, which results in a narrow time window for the ribosome nascent chain to interact productively with the membrane-bound translocation machinery. The highly negatively charged N-terminal domain of FtsY, which is a conserved feature among prokaryotic SRP receptor homologs, is important for ...
Synonyms for Escherichia coli infections in Free Thesaurus. Antonyms for Escherichia coli infections. 1 synonym for Escherichia coli: E. coli. What are synonyms for Escherichia coli infections?
How is Defective DNA of the Bacteria Escherichia Coli abbreviated? DNAa stands for Defective DNA of the Bacteria Escherichia Coli. DNAa is defined as Defective DNA of the Bacteria Escherichia Coli very rarely.
The intimin gene eae, located within the locus of enterocyte effacement pathogenicity island, distinguishes enteropathogenic Escherichia coli (EPEC) and some Shiga toxin-producing E. coli (STEC) strains from all other pathotypes of diarrheagenic E. coli. EPEC is a leading cause of infantile diarrhea in developing countries, and intimin-positive STEC isolates are typically associated with life-threatening diseases such as hemolytic-uremic syndrome and hemorrhagic colitis. Here we describe the development of a PCR-restriction fragment length polymorphism (RFLP) assay that reliably differentiates all 11 known intimin types (α1, α2, β, γ, κ, ɛ, η, ι, λ, θ, and ζ) and three new intimin genes that show less than 95% nucleotide sequence identity with existing intimin types. We designated these new intimin genes Int-μ, Int-ν, and Int-ξ. The PCR-RFLP assay was used to screen 213 eae-positive E. coli isolates derived from ovine, bovine, and human sources comprising 60 serotypes. Of these, 82 were
Abstract: This work reports for the first on the prevalence of Escherichia coli and Salmonella spp. in beef sold in the Tamale Metropolis. The conventional method was used to isolate Escherichia coli and Salmonella spp. from beef samples sold at the Tamale Metropolis. Seventy beef samples were obtained from seven different locations where meat is popularly sold in the Tamale Metropolis and analyzed microbiologically for Escherichia coli and Salmonella spp. by following procedures in the Bacteriological Analytical Manuel of the FDA-USA. The average prevalence of Escherichia coli was 56% and was highest in Location G (100%), followed by Location C (80%), Locations D and F (60%), Location B (50%) and Location E (40%). Escherichia coli was not isolated from Location A. The overall prevalence of Salmonella spp. in the beef samples was 31%. The location with the highest prevalence of Salmonella spp. was Location F (90%), followed by Location D (50%), Location E (30%) and Location C (20%). Locations A, ...
Enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are closely related pathogenic strains of Escherichia coli. The hallmark of EPEC/EHEC infections [DS:H00278 H00277] is induction of attaching and effacing (A/E) lesions that damage intestinal epithelial cells. The capacity to form A/E lesions is encoded mainly by the locus of enterocyte effacement (LEE) pathogenicity island. Tir, Map, EspF, EspG are known LEE-encoded effector proteins secreted via the type III secretion system, which is also LEE-encoded, into the host cell. EPEC and EHEC Tirs link the extracellular bacterium to the cell cytoskeleton. Map and EspF are involved in mitochondrion membrane permeabilization. EspG interacts with tubulins and stimulates microtubule destabilization. LEE-encoded adhesin or intimin (Eae) is exported via the general secretory pathway to the periplasm, where it is inserted into the outer membrane. In addition to Tir, two potential host cell-carried intimin receptors, beta1 integrin (ITGB1) ...
Enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are closely related pathogenic strains of Escherichia coli. The hallmark of EPEC/EHEC infections [DS:H00278 H00277] is induction of attaching and effacing (A/E) lesions that damage intestinal epithelial cells. The capacity to form A/E lesions is encoded mainly by the locus of enterocyte effacement (LEE) pathogenicity island. Tir, Map, EspF, EspG are known LEE-encoded effector proteins secreted via the type III secretion system, which is also LEE-encoded, into the host cell. EPEC and EHEC Tirs link the extracellular bacterium to the cell cytoskeleton. Map and EspF are involved in mitochondrion membrane permeabilization. EspG interacts with tubulins and stimulates microtubule destabilization. LEE-encoded adhesin or intimin (Eae) is exported via the general secretory pathway to the periplasm, where it is inserted into the outer membrane. In addition to Tir, two potential host cell-carried intimin receptors, beta1 integrin (ITGB1) ...
Looking for Escherichia coli infections? Find out information about Escherichia coli infections. common bacterium that normally inhabits the intestinal tracts of humans and animals, but can cause infection in other parts of the body, especially the... Explanation of Escherichia coli infections
Pathogenic Escherichia coli are known to be a common cause of diarrheal disease - a common cause of frequently occurring bacterial infections in children and adults in developing countries. It poses a significant problem in Latin America. Pathogenic Escherichia coli in Latin America presents current information on understanding pathogenic E. coli in Latin America and outlines prospects for future research in this region. It features a unique, comprehensive analysis of the most common categories of E. coli associated with diarrheal illness in Latin America. The aim of this book is to help epidemiologists in this region to learn about molecular mechanisms of E. coli pathogenesis along with its diagnosis, host immune responses, animal reservoirs and epidemiology. In addition, the authors discuss the current situation of E. coli in representative countries, including Argentina, Brazil, Chile, Mexico and Peru ...
Escherichia coli α-hemolysin (HlyA) is a pore-forming protein of 110 kDa belonging to the family of RTX toxins. A hydrophobic region between the amino acid residues 238 and 410 in the N-terminal half of HlyA has previously been suggested to form hydrophobic and/or amphipathic α-helices and has been shown to be important for hemolytic activity and pore formation in biological and artificial membranes. The structure of the HlyA transmembrane channel is, however, largely unknown. For further investigation of the channel structure, we deleted in HlyA different stretches of amino acids that could form amphipathic β-strands according to secondary structure predictions (residues 71-110, 158-167, 180-203, and 264-286). These deletions resulted in HlyA mutants with strongly reduced hemolytic activity. Lipid bilayer measurements demonstrated that HlyAΔ71-110 and HlyAΔ264-286 formed channels with much smaller single-channel conductance than wildtype HlyA, whereas their channel-forming activity was virtually
TY - JOUR. T1 - The structure of Escherichia coli heat‐stable enterotoxin b by nuclear magnetic resonance and circular dichroism. AU - Sukumar, M.. AU - Rizo-Rey, Jose. AU - Wall, M.. AU - Dreyfus, L. A.. AU - Kupersztoch, Y. M.. AU - Gierasch, L. M.. PY - 1995/9. Y1 - 1995/9. N2 - The heat‐stable enterotoxin b (STb) is secreted by enterotoxigenic Escherichia coli that cause secretory diarrhea in animals and humans. It is a 48‐amino acid peptide containing two disulfide bridges, between residues 10 and 48 and 21 and 36, which are crucial for its biological activity. Here, we report the solution structure of STb determined by two‐ and three‐dimensional NMR methods. Approximate interproton distances derived from NOE data were used to construct structures of STb using distance‐geometry and simulated annealing procedures. The NMR‐derived structure shows that STb is helical between residues 10 and 22 and residues 38 and 44. The helical structure in the region 10-22 is amphipathic and ...
Volunteer studies have shown that a 60-megadalton plasmid is required for full virulence of the human enteropathogenic Escherichia coli (EPEC) strain E2348/69 (O127:H6). The plasmid, designated pMAR2, encodes localized adherence to HEp-2 cells in tissue culture via the adhesin known as the EPEC adherence factor (EAF). Using a DNA probe for the EAF, we have previously shown that these genes are specific for EPEC and are usually encoded on plasmids ranging from 55 to 65 megadaltons. In this study, Southern blot analysis and S1 nuclease homology determination reveal a high degree of sequence conservation among these plasmids, despite some variation in restriction maps. Phenotypic characterization of the prototype EAF plasmid pMAR2 reveals that the plasmid belongs to the group IncFII and is negative for alpha-hemolysin, colicin, and aerobactin synthesis, as well as biochemical markers and antibiotic resistance. Regions encoding adherence to HEp-2 cells were localized by Tn801 insertion mutagenesis. ...
FtsA is an essential cell division protein which is synthesized in minute amounts in Escherichia coli. To study the effects of overexpressing ftsA on the phenotype of E. coli cells, DNA fragments encoding the ftsA gene were subcloned downstream of a lac or a tac promoter in two plasmids. High-level expression of the ftsA gene from these promoters inhibited normal cell septation and caused the cells to become long, nonseptate filaments. Continued overexpression of ftsA resulted in the filaments developing spherical bulges up to 4 um in diameter. It is suggested that these bulges may emanate from septation sites because they were evenly spaced in relation to one another and to the cell poles. Observations of thin sections by electron microscopy demonstrated that these bulges contained small electron dense regions and large electron-lucent plate-like inclusions. A finding that the bulging filamentous cells contain more hexosamine per mass than control cells suggests that abnormal peptidoglycan synthesis
Diarrhea caused by Escherichia coli that produce only heat-stable enterotoxin.: To determine the role of Escherichia coli heat-stable enterotoxin (ST) as a viru
ABSTRACT. Costa K.O., Alzamora Filho F., Costa J.N., Amorim C.R.N.,Yano T. & Conceição R.A. [Virulence factors of Escherichia coli isolated from calves with diarrhea in the region of Feira de Santana, Bahia.] Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarreia na região de Feira de Santana, Bahia. Revista Brasileira de Medicina Veterinária, 36(4):430-436, 2014. Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, BR 116 norte, Km 3, Feira de Santana, BA 44032-560, Brasil. E-mail: [email protected] Escherichia coli is a Gram-negative bacterium most commonly isolated from intra and extraintestinal infections in both man and in other animals such as pigs and calves. Six distinct groups of E. coli can be identified by the type of toxin and the clinical signals they produce. Among these, E. coli enterotoxigenic (ETEC) is the most commonly found and produces two types of toxin: heat-labile (LT-I and LT-II) and heat-stable (STa and ...
Escherichia coli strains of nonenteropathogenic serogroups carrying eae but lacking the enteropathogenic E. coli adherence factor plasmid and Shiga toxin DNA probe sequences were isolated from patients (children, adults, and AIDS patients) with and without diarrhea in Brazil. Although diverse in phenotype and genotype, some strains are potentially diarrheagenic ...
Looking for Escherichia coli enteritis? Find out information about Escherichia coli enteritis. common bacterium that normally inhabits the intestinal tracts of humans and animals, but can cause infection in other parts of the body, especially the... Explanation of Escherichia coli enteritis
REFERENCES. ANDRADE, G. I. et al. Identification of virulence factors by multiplex PCR in Escherichia coli isolated from calves in Minas Gerais, Brazil. Tropical Animal Health and Production, v.44, n.7, p.1783-1790, 2012. Available from: ,Available from: ,. Accessed: Nov. 20, 2013. doi: 10.1007/s11250-012-0139-8. [ Links ] BALDY-CHUDZIK, K. et al. Phylogenetic background, virulence gene profiles, and genomic diversity in commensal Escherichia coli isolated from ten mammal species living in one zoo. Veterinary Microbiology, v.131, n.1-2, p.173-184, 2008. Available from: ,Available from: ,. Accessed: Nov. 20, 2013. doi: 10.1016/j.vetmic.2008.02.019. [ Links ] BERALDO, L. G. et al. Detection of Shiga toxigenic (STEC) and enteropathogenic (EPEC) Escherichia coli in dairy buffalo. Veterinary Microbiology, v.170, n.1-2, p.162-166, 2014. Available from: ,Available from: ...
TY - JOUR. T1 - Genotipificación, evaluación toxigénica in vitro y sensibilidad a antibióticos de cepas de escherichia coli aisladas de casos diarreicos y fatales en alpacas neonatas. AU - Luna, E. Luis. AU - Maturrano, H. Lenin. AU - Rivera Geronimo, Hermelinda. AU - Zanabria, H. Víctor. AU - Rosadio, A. Raúl. N1 - Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2012/8. Y1 - 2012/8. N2 - Clinical rectal diarrheic swabs (n=27) and pathological intestinal contents (n=24) from 51 alpacas between 1 to 7 weeks of age were used to isolate and genotype Escherichia coli, and to test for antimicrobial sensibility. All the E. coli isolates, confirmed by the API system, were genotyped by Multiplex PCR to determine the presence of virulence genes: stx1 and stx2 (shigatoxin 1 and 2), eae (intimin), bfp (Bundle-Forming Pili), lt (heat-labile toxin), sta and stb (heat-stable toxin A and B), in enterohemorrhagic (EHEC), enteropathogenic (EPEC) or enterotoxigenic (ETEC) E. coli ...
No presente estudo, 47 amostras enteropatogênicas de Escherichia coli, previamente caracterizadas pelo sorotipo, fenótipo de aderência, habilidade de induzir a formação da lesão histopatológica e presença das seqüências genéticas eae, bfp e EAF, foram analisadas de acordo com o perfil de fragmentação do DNA cromossômico pela técnica de eletroforese em campo pulsado (PFGE), as variantes isoenzimáticas através da eletroforese de isoenzimas (MLEE) e a presença de seqüências específicas da região LEE (eae, espA, espB, tir) e respectivos alelos. A amplificação destas seqüências mostrou a presença de 18 padrões genéticos distintos. A tipagem do gene eae revelou que a maior parte das amostras apresentou intimina não-tipável (42%) seguida dos tipos alélicos beta (35%), gama e alfa (12% cada). A fragmentação do DNA cromossômico detectou um elevado polimorfismo genético entre as amostras estudadas e não foi observada uma correlação com os marcadores de virulência ...
Attaching and effacing (A/E) intestinal lesions are produced by enteropathogenic Escherichia coli (EPEC), enterohemorrhagic E. coli (EHEC), and RDEC-1, a pathogen of weanling rabbits. We recently identified a chromosomal locus (eae[E. coli A/E]) which is required for A/E activity in a wild-type EPEC strain. Sequences homologous to those of an eae gene probe were detected in EPEC, RDEC-1, and EHEC isolates. We report here that the eae gene is chromosomally encoded in all EPEC and EHEC strains tested and in RDEC-1. In addition, the eae probe was found to be 100% sensitive and 98% specific in detecting E. coli of EPEC serogroups that demonstrate A/E activity. Ten percent of E. coli of EPEC serogroups that hybridized with the eae probe and produced A/E activity did not hybridize with the EAF (EPEC adherence factor) probe, a plasmid-associated diagnostic probe which is currently used to identify EPEC. In addition to A/E factors, plasmid-associated adhesins also contribute to the pathogenesis of EPEC ...
Summary Strains of Escherichia coli from sporadic cases of diarrhoea and belonging to serotypes O44:H18, O55:H7, O111ab:H21 O111ab:H25 or O126:H25 or O126:H27 were examined for virulence properties. With the exception of O111ab:H25 these are considered to be classical enteropathogenic E. coli (EPEC) serotypes. The strains had been isolated in Britain from the faeces of children |3 years old. Of the serotypes examined, 7 of 13 O44:H18 strains, all of 10 O111ab:H21 strains and 13 of 21 O126:H27 strains belonged to the enteroaggregative class of E. coli (EAggEC) that attached to HEp-2 cells in the characteristic aggregative pattern and hybridised with the EAggEC probe. They also caused mannose-resistant haemagglutination of rat erythrocytes, a property which may be a useful marker for their identification. Strains of O44:H18 with similar properties were also isolated from three small outbreaks in Britain, one of which involved elderly patients. EAggEC have not been considered previously as aetiological
TY - JOUR. T1 - Draft genome sequences of Escherichia coli strains isolated from septic patients. AU - Dunitz, Madison I.. AU - Coil, David A.. AU - Jospin, Guillaume. AU - Eisen, Jonathan A. AU - Adams, Jason Yeates. PY - 2014. Y1 - 2014. N2 - We present the draft genome sequences of six strains of Escherichia coli isolated from blood cultures collected from patients with sepsis. The strains were collected from two patient sets, those with a high severity of illness, and those with a low severity of illness. Each genome was sequenced by both Illumina and PacBio for comparison.. AB - We present the draft genome sequences of six strains of Escherichia coli isolated from blood cultures collected from patients with sepsis. The strains were collected from two patient sets, those with a high severity of illness, and those with a low severity of illness. Each genome was sequenced by both Illumina and PacBio for comparison.. UR - ...
TY - JOUR. T1 - Recovery of YAC-end sequences through complementation of an Escherichia coli pyrF mutation. AU - Wright, David A.. AU - Park, Sei Kyoung. AU - Wu, Dongying. AU - Phillips, Gregory J.. AU - Rodermel, Steven R.. AU - Voytas, Daniel F.. PY - 1997/7/1. Y1 - 1997/7/1. N2 - We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli gene required for pyrimidine biosynthesis. We have developed an E. coli strain with a non-reverting allele of pyrF that is also suitable for cloning (recA-, hsdR-). We demonstrate the utility of this complementation strategy to obtain right-end clones from three YACs containing Arabidopsis thaliana DNA.. AB - We have developed a genetic means to recover sequences from YAC-ends near the yeast selectable marker URA3. This strategy is based on the ability of URA3 to complement mutations in pyrF, an Escherichia coli ...
α-helical membrane proteins constitute 20-30% of all proteins in a cell and are involved in many essential cellular functions. The structure is only known for a few hundred of them, which makes structural models important. The most common structural model of a membrane protein is the topology which is a two-dimensional representation of the structure.. This thesis is focused on three different aspects of membrane protein structure: improving structural predictions of membrane proteins, improving the level of detail of structural models and the concept of dual topology.. It is possible to improve topology models of membrane proteins by including experimental information in computer predictions. This was first performed in Escherichia coli and, by using homology, it was possible to extend the results to 225 prokaryotic organisms. The improved models covered ~80% of the membrane proteins in E. coli and ~30% of other prokaryotic organisms.. However, the traditional topology concept is sometimes too ...
Eenteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) are closely related pathogenic strains of Escherichia coli. The hallmark of EPEC/EHEC infections is induction of attaching and effacing (A/E) lesions that damage intestinal epithelial cells. The capacity to form A/E lesions is encoded mainly by the locus of enterocyte effacement (LEE) pathogenicity island. Tir, Map, EspF, EspG are known LEE-encoded effector proteins secreted via the type III secretion system, which is also LEE-encoded, into the host cell. EPEC and EHEC Tirs link the extracellular bacterium to the cell cytoskeleton. Map and EspF are involved in mitochondrion membrane permeabilization. EspG interacts with tubulins and stimulates microtubule destabilization. LEE-encoded adhesin or intimin (Eae) is exported via the general secretory pathway to the periplasm, where it is inserted into the outer membrane. In addition to Tir, two potential host cell-carried intimin receptors, beta1 integrin (ITGB1) and nucleolin ...
LAMPRECHT, Corne et al. Escherichia coli with virulence factors and multidrug resistance in the Plankenburg River. S. Afr. j. sci. [online]. 2014, vol.110, n.9-10, pp.01-06. ISSN 1996-7489. Escherichia coli is a natural inhabitant of the gut and E. coli levels in water are considered internationally to be an indication of faecal contamination. Although not usually pathogenic, E. coli has been linked to numerous foodborne disease outbreaks, especially those associated with fresh produce. One of the most common ways through which E. coli can be transferred onto fresh produce is if contaminated water is used for irrigation. In this study, a total of 81 confirmed E. coli strains were isolated from the Plankenburg River as part of three separate studies over 3 years. During sampling, E. coli levels in the river were above the accepted levels set by the World Health Organization and the South African Department of Water Affairs and Forestry for safe ...
DksA is a global regulatory protein that, together with the alarmone ppGpp, is required for the stringent response to nutrient starvation in the gammaproteobacterium Escherichia coli and for more moderate shifts between growth conditions. DksA modulates the expression of hundreds of genes, directly or indirectly. Mutants lacking a DksA homolog exhibit pleiotropic phenotypes in other gammaproteobacteria as well. Here we analyzed the DksA homolog RSP2654 in the more distantly related Rhodobacter sphaeroides, an alphaproteobacterium. RSP2654 is 42% identical and similar in length to E. coli DksA but lacks the Zn finger motif of the E. coli DksA globular domain. Deletion of the RSP2654 gene results in defects in photosynthetic growth, impaired utilization of amino acids, and an increase in fatty acid content. RSP2654 complements the growth and regulatory defects of an E. coli strain lacking the dksA gene and modulates transcription in vitro with E. coli RNA polymerase (RNAP) similarly to E. coli ...
TY - JOUR. T1 - Development of glycerol-utilizing Escherichia coli strain for the production of bioethanol. AU - Thapa, Laxmi Prasad. AU - Lee, Sang Jun. AU - Yoo, Hah Young. AU - Choi, Han Suk. AU - Park, Chulhwan. AU - Kim, Seung Wook. PY - 2013/8/15. Y1 - 2013/8/15. N2 - production of bioethanol was studied using recombinant Escherichia coli with glycerol as a carbon source. Glycerol is an attractive feedstock for biofuels production since it is generated as a major byproduct in biodiesel industry; therefore, we investigated the conversion of glycerol to bioethanol using E. coli BL21 (DE3) which harbors several genes in ethanol production pathway of Enterobacter aerogenes KCTC 2190. Fermentation was carried out at 34 °C for 42. h, pH 7.6, using defined production medium. Under optimal conditions, bioethanol production by the recombinant E. coli BL21 (DE3), strain pEB, was two-fold (3.01. g/L) greater than that (1.45. g/L) by the wild-type counterpart. The results obtained in this study will ...
TY - JOUR. T1 - An nad synthetic reaction bypasses the lipoate requirement for aerobic growth of Escherichia coli strains blocked in succinate catabolism. AU - Hermes, Fatemah A.. AU - Cronan, John E.. PY - 2014/12/1. Y1 - 2014/12/1. N2 - The lipoate coenzyme is essential for function of the pyruvate (PDH) and 2-oxoglutarate (OGDH) dehydrogenases and thus for aerobic growth of Escherichia coli. LipB catalyzes the first step in lipoate synthesis, transfer of an octanoyl moiety from the fatty acid synthetic intermediate, octanoyl-ACP, to PDH and OGDH. E. coli also encodes LplA, a ligase that in presence of exogenous octanoate (or lipoate) can bypass loss of LipB. LplA imparts ΔlipB strains with a leaky growth phenotype on aerobic glucose minimal medium supplemented with succinate (which bypasses the OGDH-catalyzed reaction), because it scavenges an endogenous octanoate pool to activate PDH. Here we characterize a ΔlipB suppressor strain that did not require succinate supplementation, but did ...
RecA is important for recombination, DNA repair, and SOS induction. In Escherichia coli, RecBCD, RecFOR, and RecJQ prepare DNA substrates onto which RecA binds. UvrD is a 3-to-5 helicase that participates in methyl-directed mismatch repair and nucleotide excision repair. uvrD deletion mutants are sensitive to UV irradiation, hypermutable, and hyper-rec. In vitro, UvrD can dissociate RecA from single-stranded DNA. Other experiments suggest that UvrD removes RecA from DNA where it promotes unproductive reactions. To test if UvrD limits the number and/or the size of RecA-DNA structures in vivo, an uvrD mutation was combined with recA-gfp. This recA allele allows the number of RecA structures and the amount of RecA at these structures to be assayed in living cells. uvrD mutants show a threefold increase in the number of RecA-GFP foci, and these foci are, on average, nearly twofold higher in relative intensity. The increased number of RecA-green fluorescent protein foci in the uvrD mutant is ...
Escherichia coli Hsp31, encoded by hchA, is a heat-inducible molecular chaperone. We found that Hsp31 undergoes a conformational change via temperature-induced unfolding, generating a high molecular weight (HMW) form with enhanced chaperone activity. Although it has previously been reported that some subunits of the Hsp31 crystal structure show structural heterogeneity with increased hydrophobic surfaces, Hsp31 basically forms a dimer. We found that a C-terminal deletion (C Delta 19) of Hsp31 exhibited structurally and functionally similar characteristics to that of the HMW form. Both the C Delta 19 and HMW forms achieved a structure with considerably more p-sheets and less a-helices than the native dimeric form, exposing a portion of its hydrophobic surfaces. The structural alterations were determined from its spectral changes in circular dichroism, intrinsic fluorescence of tryptophan residues, and fluorescence of bis-ANS binding to a hydrophobic surface. Interestingly, during thermal ...
Principal Investigator:SUGAI Motoyuki, Project Period (FY):1997 - 1998, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Bacteriology (including Mycology)
The Tat system transports folded proteins across the bacterial cytoplasmic membrane and the thylakoid membrane of plant chloroplasts. Substrates are targeted to the Tat pathway by signal peptides containing a pair of consecutive arginine residues. The membrane proteins TatA, TatB and TatC are the essential components of this pathway in Escherichia coli. The complexes that these proteins form at native levels of expression have been investigated by the use of affinity tag-coding sequences fused to chromosomal tat genes. Distinct TatA and TatBC complexes were identified using size-exclusion chromatography and shown to have apparent molecular masses of approximately 700 and 500 kDa, respectively. Following in vivo expression, the Tat substrate protein SufI was found to copurify with the TatBC, but not the TatA, complex. This binding required the SufI signal peptide. Substitution of the twin-arginine residues in the SufI signal peptide by either twin lysine or twin alanine residues abolished export. However
Attaching and effacing (AE) adhesion is associated with the pathogenesis of enteropathogenic Escherichia coli (EPEC) and rabbit diarrheogenic E. coli (RDEC-1). Although RDEC-1 provides an animal model for investigating pathophysiology of EPEC infection, RDEC-1 does not adhere to human cell lines, thereby limiting in vitro investigation. Therefore, transformed RDEC-1 strains expressing adhesins derived from human diarrheogenic E. coli were studied. These adhesins promoted AE adhesion of RDEC-1 and led to the accumulation of alpha-actinin aggregates in the cytoplasm of infected cells. Furthermore, these strains induced host signal transduction pathways, resulting in tyrosine phosphorylation of host proteins and an intracellular elevation of calcium. These results demonstrate that RDEC-1 and EPEC stimulate similar signal transduction pathways in infected epithelial cells, thus lending additional support for the use of RDEC-1 as a model for the study of human EPEC infection.
TY - JOUR. T1 - Effect of the ε-subunit on nucleotide binding to Escherichia coli F1- ATPase catalytic sites. AU - Weber, Joachim. AU - Dunn, Stanley D.. AU - Senior, Alan E.. PY - 1999/7/2. Y1 - 1999/7/2. N2 - The influence of the ε-subunit on the nucleotide binding affinities of the three catalytic sites of Escherichia coli F1-ATPase was investigated, using a genetically engineered Trp probe in the adenine-binding subdomain (β-Trp-331). The interaction between ε and F1 was not affected by the mutation. K(d) for binding of ε to βY331W mutant F1 was ~1 nM, and ε inhibited ATPase activity by 90%. The only nucleotide binding affinities that showed significant differences in the ε-depleted and ε-replete forms of the enzyme were those for MgATP and MgADP at the high-affinity catalytic site 1. K(d1)(MgATP) and K(d1)(MgADP) were an order of magnitude higher in the absence of ε than in its presence. In contrast, the binding affinities for MgATP and MgADP at sites 2 and 3 were similar in the ...
TY - JOUR. T1 - Phosphoryl transfer from α-D-glucose 1-phosphate catalyzed by Escherichia coli sugar-phosphate phosphatases of two protein superfamily types. AU - Wildberger, Patricia. AU - Pfeiffer, Martin. AU - Brecker, Lothar. AU - Rechberger, Gerald N.. AU - Birner-Gruenberger, Ruth. AU - Nidetzky, Bernd. PY - 2015. Y1 - 2015. N2 - The Cori ester α-D-glucose 1-phosphate (αGlc 1-P) is a high-energy intermediate of cellular carbohydrate metabolism. Its glycosidic phosphomonoester moiety primes αGlc 1-P for flexible exploitation in glucosyl and phosphoryl transfer reactions. Two structurally and mechanistically distinct sugar-phosphate phosphatases from Escherichia coli were characterized in this study for utilization of αGlc 1-P as a phosphoryl donor substrate. The agp gene encodes a periplasmic αGlc 1-P phosphatase (Agp) belonging to the histidine acid phosphatase family. Had13 is from the haloacid dehydrogenase-like phosphatase family. Cytoplasmic expression of Agp (in E. coli Origami ...
Introduction. Escherichia coli, better known as E. coli, are Gram negative, rod-shaped bacteria belonging to the family Enterobacteriaceae. Escherichia species are also included in the group of genera referred to as the coliforms.. Not all E. coli cause disease: the species is found as part of the normal, healthy human gut flora as well as in the environment. Therefore the presence of E. coli in processed food products can indicate faecal contamination. For this reason E. coli is widely used as an indicator organism for the presence of potentially more dangerous bacteria, such as Salmonella.. Although most strains of E. coli do not cause illness, some have been associated with human infections resulting in diarrhoea, or occasionally more severe illness. There are at least six different groups of diarrhoea-causing E. coli, but only two types are associated with foodborne disease.. 1. Verocytotoxin-producing (VTEC), sometimes referred to as Shiga-like toxin-producing (STEC). This group includes ...
Escherichia coli (/ˌɛʃəˈrɪkiə ˈkoʊlɪ/ Anglicized to /ˌɛʃəˈrɪkiə ˈkoʊlaɪ/; commonly abbreviated E. coli) is a gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes are pathogenic and can cause serious food poisoning in humans, and are occasionally responsible for product recalls. E. coli are also responsible for a majority of cases of urinary tract infections. The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and by preventing the establishment of pathogenic bacteria within the intestine. E. coli and related bacteria constitute about 0.1% of gut flora, and fecal-oral transmission is the major route through which pathogenic strains of the bacterium cause disease. Cells are able to survive outside the body for a limited amount of time, which makes them ideal indicator organisms to test ...
in Advances in Experimental Medicine and Biology (1999), 473. Enteropathogenic Escherichia coli (EPEC) produce attaching and effacing lesions. The genes responsible for this lesion are clustered on the chromosome forming a 35.5 kilobase pathogenesis island called ... [more ▼]. Enteropathogenic Escherichia coli (EPEC) produce attaching and effacing lesions. The genes responsible for this lesion are clustered on the chromosome forming a 35.5 kilobase pathogenesis island called LEE. The LEE was identified, characterized and completely sequenced from the human EPEC strain E2348/69. The LEE carries genes coding for: a type III secretion system (genes esc and sep), the translocated intimin receptor (gene tir), the outer membrane protein intimin (gene eae) and the E. coli secreted proteins EspA, EspB, and EspD (genes esp). In addition to man and farm animals, EPEC are also isolated from dogs and cats. We studied structurally and functionally the LEE of dog and cat EPEC. First, we used four probes ...
The increase in antibiotic resistance has become a major health concern in recent times. It is therefore essential to identify novel antibacterial targets as well as discover and develop new antibacterial agents. FtsZ, a highly conserved bacterial protein, is responsible for the initiation of cell division in bacteria. The functions of FtsZ inside cells are tightly regulated and any perturbation in its functions leads to inhibition of bacterial division. Recent reports indicate that small molecules targeting the functions of FtsZ may be used as leads to develop new antibacterial agents. To identify small molecules targeting FtsZ and inhibiting bacterial division, we screened a U.S. FDA (Food and Drug Administration)-approved drug library of 800 molecules using an independent computational, biochemical and microbial approach. From this screen, we identified doxorubicin, an anthracycline molecule that inhibits Escherichia coli division and forms filamentous cells. A fluorescence-binding assay ...
Biofilms are communities of surface-adherent bacteria surrounded by secreted polymers known as the extracellular polymeric substance. Biofilms are harmful in many industries, and thus it is of great interest to understand their mechanical properties and structure to determine ways to destabilize them. By performing single particle tracking with beads of varying surface functionalization it was found that charge interactions play a key role in mediating mobility within biofilms. With a combination of single particle tracking and microrheological concepts, it was found that Escherichia coli biofilms display height dependent charge density that evolves over time. Statistical analyses of bead trajectories and confocal microscopy showed inter-connecting micron scale channels that penetrate throughout the biofilm, which may be important for nutrient transfer through the system. This methodology provides significant insight into a particular biofilm system and can be applied to many others to provide ...
SlyA is a member of the MarR family of bacterial transcriptional regulators. Previously, SlyA has been shown to directly regulate only two operons in Escherichia coli K-12 MG1655, fimB and hlyE (clyA). In both cases, SlyA activates gene expression by antagonizing repression by the nucleoid-associated protein H-NS. Here, the transcript profiles of aerobic glucose-limited steady-state chemostat cultures of E. coli K-12 MG1655, slyA mutant and slyA over-expression strains are reported. The transcript profile of the slyA mutant was not significantly different from that of the parent; however, that of the slyA expression strain was significantly different from that of the vector control. Transcripts representing 27 operons were increased in abundance, whereas 3 were decreased. Of the 30 differentially regulated operons, 24 have previously been associated with sites of H-NS binding, suggesting that antagonism of H-NS repression is a common feature of SlyA-mediated transcription regulation. Direct binding of
Risk Assessment of Escherichia coli Infection from Use of Interactive Waterscape Facilities - Escherichia coli;Exposure;Interactive fountain;Risk assessment;
TY - JOUR. T1 - Shotgun optical maps of the whole Escherichia coli 0157. T2 - H7 genome. AU - Lim, Alex. AU - Dimalanta, Eileen T.. AU - Potamousis, Konstantinos D.. AU - Yen, Galex. AU - Apodoca, Jennifer. AU - Tao, Chunhong. AU - Lin, Jieyi. AU - Qi, Rong. AU - Skiadas, John. AU - Ramanathan, Arvind. AU - Perna, Nicole T.. AU - Plunkett, Guy. AU - Burland, Valerie. AU - Mau, Bob. AU - Hackett, Jeremiah. AU - Blattner, Frederick R.. AU - Anantharaman, Thomas S.. AU - Mishra, Bhubaneswar. AU - Schwartz, David C.. PY - 2001. Y1 - 2001. N2 - We have constructed Nhel and Xhol optical maps of Escherichia coli O157:H7 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. E. coli O157:H7 is a common pathogen found in contaminated food and water. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, because maps were constructed from ensembles of single DNA molecules. Shotgun sequencing of bacterial ...
We have found that temperature can have a striking effect upon protein export in Escherichia coli, suggesting that there is a cold-sensitive step in the protein export pathway. Cs mutations comprise the largest class of mutations affecting the membrane-localized Sec proteins SecD, SecE, SecF and SecY. Although some of these mutations could encode cold-labile proteins, this is unlikely to account for the Cs phenotype of most export mutants, as mutations which simply produce lower amounts of SecE protein have the same phenotype. Certain signal sequence mutations affecting maltose binding protein are also cold sensitive for export. These effects appear to arise by a specific interaction of cold with certain export defects. We believe that the Cs sec mutations are representative of a large class of conditional lethal mutations, whose conditional phenotype reflects an underlying thermal sensitivity of the process in which they are involved. ...
... in Escherichia coli, OriC. Additionally the protein plays a further role in sequestration. The importance of this protein is ... Slomińska M, Wegrzyn A, Konopa G, Skarstad K, Wegrzyn G (June 2001). "SeqA, the Escherichia coli origin sequestration protein, ... Waldminghaus T, Skarstad K (May 2009). "The Escherichia coli SeqA protein". Plasmid. 61 (3): 141-50. doi:10.1016/j.plasmid. ... This protein domain is thought to be part of a much larger protein complex which includes other proteins such as SeqB. DNA ...
Baneyx F (October 1999). "Recombinant protein expression in Escherichia coli". Current Opinion in Biotechnology. 10 (5): 411-21 ... Gene expression Single-cell protein Protein purification Precision fermentation Host cell protein List of recombinant proteins ... E. coli strain BL21 and BL21(DE3) are two strains commonly used for protein production. As members of the B lineage, they lack ... Protein production is the biotechnological process of generating a specific protein. It is typically achieved by the ...
"Cyclization recombinase [Escherichia coli] - Protein - NCBI". Sternberg N, Hamilton D (August 1981). "Bacteriophage P1 site- ... Protein-protein interactions drive and direct strand exchange. Energy is not compromised, since the protein-DNA linkage makes ... Cre recombinase proteins bind to the first and last 13 bp regions of a lox site forming a dimer. This dimer then binds to a ... The total protein has 343 amino acids. The C domain is similar in structure to the domain in the Integrase family of enzymes ...
Goldstein J, Pollitt NS, Inouye M (January 1990). "Major cold shock protein of Escherichia coli". Proc. Natl. Acad. Sci. U.S.A ... Brandi A, Spurio R, Gualerzi CO, Pon CL (March 1999). "Massive presence of the Escherichia coli 'major cold-shock protein' CspA ... untranslated region of the cold shock cspA mRNA of Escherichia coli". J. Bacteriol. 181 (20): 6284-6291. doi:10.1128/JB.181.20. ... cspA protein is then produced in significantly higher quantities, making up over 2% of the cell's proteome during cold shock. ...
Protein Data Bank. doi:10.2210/pdb3fg9/pdb.. Siegele DA, et al. (2005). "Universal Stress Proteins in Escherichia coli". ... Diez A (2002). "The universal stress protein A of Escherichia coli is required for resistance to DNA damaging agents and is ... Gustavsson N (2002). "The universal stress protein paralogues of Escherichia coli are co-ordinately regulated and co-operate in ... 2005). "Differential Roles of the Universal Stress Proteins of Escherichia coli in Oxidative Stress Resistance, Adhesion, and ...
Chowdhury S, Hepper S, Lodi MK, Saier MH, Uetz P (April 2021). "The Protein Interactome of Glycolysis in Escherichia coli". ... Allosteric inhibition and activation by Protein-protein interactions (PPI). Indeed, some proteins interact with and regulate ... PFK2 is phosphorylated by protein kinase A. The phosphorylation inactivates PFK2, and another domain on this protein becomes ... The diagram below shows human protein names. Names in other organisms may be different and the number of isozymes (such as HK1 ...
Emr, Scott David (1981). Protein localization in Escherichia coli (PhD). Harvard University. Retrieved November 6, 2021. "Scott ... protein transport in cells by vesicles and the role of arrestin and ubiquitylation in the degradation of membrane proteins. ... ESCRTs are required for the degradation of membrane protein at the lysosome, a late step in cytokinesis, and the budding and ... Suzuki, Sho W.; Emr, Scott D. (2018). "Membrane protein recycling from the vacuole/lysosome membrane". Journal of Cell Biology ...
Chowdhury S, Hepper S, Lodi MK, Saier MH, Uetz P (April 2021). "The Protein Interactome of Glycolysis in Escherichia coli". ... This is in contrast to another model organism, Escherichia coli, in which only 15% of its metabolic enzymes are essential. In ... This may be due to the fact that Mycoplasma pneumoniae's metabolome is less efficient than that of Escherichia coli. The ... Another protein considered to play an important role in adherence is P30, as M. pneumoniae cells with mutations in this protein ...
Enterotoxigenic Escherichia coli uses a N-glycosyltransferase called EtpC to modify the EtpA protein, which is orthologous to ... November 2017). "A biosynthetic route for polysialylating proteins in Escherichia coli". Metabolic Engineering. 44: 293-301. ... and later in other bacterial species such as Escherichia coli. N-glycosyltransferases usually target adhesin proteins, which ... The N-glycosyltransferases are subdivided into two functional classes, the first (e.g several Yersinia, Escherichia coli and ...
Foster, D. L.; Boublik, M.; Kaback, H. R. (1983). "Structure of the lac carrier protein of Escherichia coli". J. Biol. Chem. ... Guan, L; Weinglass, AB; Kaback, HR (7 September 2001). "Helix packing in the lactose permease of Escherichia coli: localization ... "Site-directed sulfhydryl labeling of the lactose permease of Escherichia coli: helices IV and V that contain the major ... "The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles". Proc. ...
Tissieres, A.; Schlessinger, D.; Gros, Françoise (1960). "Amino Acid Incorporation into Proteins by Escherichia coli Ribosomes ... Srivastava, A. K.; Schlessinger, D. (1988). "Coregulation of processing and translation: Mature 5' termini of Escherichia coli ... Schlessinger, David (1960). "Hypochromicity in ribosomes from Escherichia coli". Journal of Molecular Biology. 2 (2): 92-95. ... Luzzatto, Lucio; Schlessinger, David; Apirion, David (1968). "Escherichia coli : High Resistance or Dependence on Streptomycin ...
1983). Outer membrane protein K of Escherichia coli: purification and pore-forming properties in lipid bilayer membranes. J ... Sutcliffe, J; Blumenthal, R; Walter, A; Foulds, J. "Escherichia coli outer membrane protein K is a porin". Journal of ... Escherichia coli outer membrane protein K is a porin. J Bacteriol 156(2): 867-872 Bliss JM, Solver RP. (1996). Coating the ... a model for expression of capsular polysialic acid in Escherichia coli K1. Mol Microbiol 21:221. v t e (Outer membrane proteins ...
Foster DL, Boublik M, Kaback HR (January 1983). "Structure of the lac carrier protein of Escherichia coli". The Journal of ... Protein domains, Transmembrane proteins, Articles containing video clips, Protein superfamilies, Transport proteins). ... Yin Y, He X, Szewczyk P, Nguyen T, Chang G (May 2006). "Structure of the multidrug transporter EmrD from Escherichia coli". ... Västermark A, Lunt B, Saier M (2014). "Major facilitator superfamily porters, LacY, FucP and XylE of Escherichia coli appear to ...
"Heat shock protein 90 from Escherichia coli collaborates with the DnaK chaperone system in client protein remodeling. Proc Natl ... S. Wickner (1978) "DNA Replication Proteins of Escherichia coli." Annu Review of Biochem. 78: 1163-1191. "Sue Wickner". Albert ... coli and chaperone function in yeast." Mol. Cell. 49(3):464-473. S. M. Doyle, O. Genest, and S. Wickner (2013) "Protein rescue ... and break down proteins. She has been a major contributor to the understanding of molecular chaperones, proteins that regulate ...
Milkman R (Apr 1994). "An Escherichia coli homologue of eukaryotic potassium channel proteins". Proceedings of the National ... Jiang Y, Pico A, Cadene M, Chait BT, MacKinnon R (Mar 2001). "Structure of the RCK domain from the E. coli K+ channel and ... or may be through an additional calcium binding protein such as calmodulin. Knowing the structure of these channels can provide ... "Gating of the TrkH ion channel by its associated RCK protein TrkA". Nature. 496 (7445): 317-22. Bibcode:2013Natur.496..317C. ...
In Escherichia coli Dps protein is Induced by rpoS and IHF in the early stationary phase. Dps is also Induced by oxyR in ... A ferritin-like DNA-binding protein of Escherichia coli". The Journal of Biological Chemistry. 277 (31): 27689-96. doi:10.1074/ ... The first was discovered in Escherichia coli Dps in 1992 and has given the name to the protein family; during stationary phase ... "A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli". Genes & Development. 6 (12B): ...
... prismane protein') from Escherichia coli. Characterization of the hybrid-cluster protein, redox properties of the [2Fe-2S] and ... Protein engineering of the CODH/ACS in M.thermoacetica revealed that mutating residues, so as to functionally block the tunnel ... Role of a 22-kDa iron-sulfur protein in mediating electron transfer between carbon monoxide dehydrogenase and hydrogenase". The ... van den Berg WA, Hagen WR, van Dongen WM (February 2000). "The hybrid-cluster protein (' ...
Hengen, Paul N (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences. 20 (7 ... For example, even when a recombinant protein forcibly expressed in E. coli produces an inclusion body and can not be obtained ... are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other ... For proteins with a single hexahistidine tag, 75 mM imidazole enables elution from Ni-NTA, whereas for proteins with two ...
Meyer RR, Laine PS (December 1990). "The single-stranded DNA-binding protein of Escherichia coli". Microbiol. Rev. 54 (4): 342- ... "Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined ... Protein heteropolymers, Protein families, DNA replication, Proteins, DNA-binding substances). ... Single-stranded binding proteins (SSBs) are a class of proteins that have been identified in both viruses and organisms from ...
Escherichia coli can fix these modifications using AlkB protein. Another way to produce it is via cyclisation of N6- ... N6-ethenoadenine in Escherichia coli cells". Mutagenesis. 26 (3): 401-406. doi:10.1093/mutage/geq107. PMID 21193516. Hang, B ( ... MacIejewska, A. M.; Sokolowska, B.; Nowicki, A.; Kusmierek, J. T. (2011). "The role of AlkB protein in repair of 1, ...
Milkman R (April 1994). "An Escherichia coli homologue of eukaryotic potassium channel proteins". Proceedings of the National ... Protein domains, Protein families, Transmembrane proteins, Ion channels). ... The HVCN1 and Putative tyrosine-protein phosphatase proteins do not contain an expected ion conduction pore domain, but rather ... The proteins with only two transmembrane helices (Pfam PF07885) are most commonly found in bacteria. This also includes the 2- ...
Wang JC (February 1971). "Interaction between DNA and an Escherichia coli protein omega". Journal of Molecular Biology. 55 (3 ... it is now called Escherichia coli (E. coli) topoisomerase I (topo I) and is a representative of the type IA family of enzymes. ... These include YacG and pentapeptide repeat proteins, such as QnrB1 and MfpA; these protein inhibitors also confer resistance to ... purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme". ...
... (SSB) is a protein found in Escherichia coli (E. coli) bacteria, that binds to single- ... February 2014). "Intramolecular binding mode of the C-terminus of Escherichia coli single-stranded DNA binding protein ... Meyer RR, Laine PS (December 1990). "The single-stranded DNA-binding protein of Escherichia coli". Microbiological Reviews. 54 ... "Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined ...
Squires, C L; Pedersen, S; Ross, B M; Squires, C (1991). "ClpB is the Escherichia coli heat shock protein F84.1". Journal of ... Marr, Allen G.; Ingraham, John L.; Squires, Craig L. (1964). "EFFECT OF THE TEMPERATURE OF GROWTH OF ESCHERICHIA COLI ON THE ... Catherine Louise Kearney Squires was a microbiologist known for her work on ribosomal RNA using Escherichia coli as a model ... Squires' research established a mutant of Escherichia coli (strain Δ7) which had all seven of its rrn operons removed. Squires ...
Peter B. Crowley; Elysian Chow; Tatiana Papkovskaia (2011). "Protein Interactions in the Escherichia coli Cytosol: An ... when measured inside cells of Escherichia coli. In particular, these proteins seemed to tumble as if they had molecular weights ... "Physicochemical code for quinary protein interactions in Escherichia coli". Proceedings of the National Academy of Sciences of ... the presence of many hydrophobic residues in the protein surface would slow down protein intracellular tumbling. Protein dipole ...
The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and ... Morris TW, Reed KE, Cronan JE (June 1994). "Identification of the gene encoding lipoate-protein ligase A of Escherichia coli. ... Lipoate-protein ligase (EC, LplA, lipoate protein ligase, lipoate-protein ligase A, LPL, LPL-B) is an enzyme with ... "Crystal structure of lipoate-protein ligase A from Escherichia coli. Determination of the lipoic acid-binding site". The ...
Zengel JM, Lindahl L (1994). Diverse mechanisms for regulating ribosomal protein synthesis in Escherichia coli. Progress in ... Page for Ribosomal protein L10 leader at Rfam v t e (Ribosomal protein leader, All stub articles, Molecular and cellular ... Other ribosomal protein leaders identified in the same study include those of L13, L19, L20 and L21. ... It is located in the 5′ untranslated regions of mRNAs encoding ribosomal proteins L10 and L12 (rplJ-rplL). A Rho-independent ...
Choi JH, Lee SY (June 2004). "Secretory and extracellular production of recombinant proteins using Escherichia coli". Appl. ... Joseleau-Petit D, Liébart JC, Ayala JA, D'Ari R (September 2007). "Unstable Escherichia coli L Forms Revisited: Growth Requires ... such as Bacillus subtilis or Escherichia coli. This is done by inhibiting peptidoglycan synthesis with antibiotics or treating ... Here, the absence of a cell wall can allow production of large amounts of secreted proteins that would otherwise accumulate in ...
It is commonly used for protein production in Escherichia coli. Two hybrid promoters functional in Escherichia coli were ... "Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli". Gene. 69 ( ... This makes it suitable for high-efficiency protein production of a recombinant protein. The strong repression of expression in ... "Maltose Binding Protein Expression , NEB". Retrieved 2016-02-26. Human artificial chromosome Yeast artificial ...
Examples include the HU protein in Escherichia coli, a dimer of closely related alpha and beta chains and in other bacteria can ... Kim EA, Blair DF (October 2015). "Function of the Histone-Like Protein H-NS in Motility of Escherichia coli: Multiple ... The role of single-stranded DNA binding (SSB) protein during DNA replication in Escherichia coli cells has been studied, ... Balandina A, Claret L, Hengge-Aronis R, Rouviere-Yaniv J (February 2001). "The Escherichia coli histone-like protein HU ...
The trial intends to evaluate LBP-EC01, a CRISPR Cas3-enhanced bacteriophage against Escherichia coli bacteria which cause ... Class 2 systems use a single large Cas protein for the same purpose. Class 1 is divided into types I, III, and IV; class 2 is ... Class 1 systems use a complex of multiple Cas proteins to degrade foreign nucleic acids. ...
Kelly TM, Stachula SA, Raetz CR, Anderson MS (September 1993). "The firA gene of Escherichia coli encodes UDP-3-O-(R-3- ... This enzyme catalyses the following chemical reaction (3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3- ... Bartling CM, Raetz CR (September 2009). "Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N- ... acyl-carrier protein):UDP-3-O-((3R)-3-hydroxymyristoyl)-alpha-D-glucosamine N-acetyltransferase. ...
The most common bacteria that make this enzyme are gram-negative such as Escherichia coli and Klebsiella pneumoniae, but the ... Protein pages needing a picture, Articles containing potentially dated statements from June 2010, All articles containing ... In May 2010, a case of infection with E. coli expressing NDM-1 was reported in Coventry in the United Kingdom. The patient was ...
The crystal structure of the MenH enzyme in E.coli (SHCHC synthase) exists as a complex of three protein molecules shown in the ... also known as SHCHC synthase is encoded by the menH gene in Escherichia coli and functions in the synthesis of vitamin K. The ... "Identification of a hotdog fold thioesterase involved in the biosynthesis of menaquinone in Escherichia coli". Journal of ... "Catalytic mechanism of SHCHC synthase in the menaquinone biosynthesis of Escherichia coli: identification and mutational ...
The enzyme from Escherichia coli consists of two alpha8-beta8 (TIM) barrels positioned face to face and thought to have evolved ... Protein families, All stub articles, EC 2.1 stubs). ... binding site in methionine synthase enzymes of Escherichia coli ... "Transfer of the methyl group from N5-methyltetrahydrofolates to homocysteine in Escherichia coli" (Free full text). The ... "Cobalamin-independent methionine synthase from Escherichia coli: a zinc metalloenzyme". Biochemistry. 35 (38): 12228-34. doi: ...
Kato J, Katayama T (August 2001). "Hda, a novel DnaA-related protein, regulates the replicgation cycle in Escherichia coli". ... a novel DnaA-binding protein, ensures the timely initiation of Escherichia coli chromosome replication". The Journal of ... In E. coli these proteins include DiaA, SeqA, IciA, HU, and ArcA-P, but they vary across other bacterial species. A few other ... Termination of DNA replication in E. coli is completed through the use of termination sequences and the Tus protein. These ...
Light S, Kraulis P (February 2004). "Network analysis of metabolic enzyme evolution in Escherichia coli". BMC Bioinformatics. 5 ... Proteins are made of amino acids arranged in a linear chain joined by peptide bonds. Many proteins are enzymes that catalyze ... In prokaryotes, these proteins are found in the cell's inner membrane. These proteins use the energy from reduced molecules ... Amino acids are made into proteins by being joined in a chain of peptide bonds. Each different protein has a unique sequence of ...
Thanks to the similarity among the gene content of Buchnera aphidicola and the enteric bacteria Escherichia coli, 89% identity ... the microsporidia shrunk its genome eliminating almost 1000 genes and reduced even the size of protein and protein-coding genes ... "The Complete Genome Sequence of Escherichia coli K-12". Science. 277 (5331): 1453-1462. doi:10.1126/science.277.5331.1453. ISSN ... The genome of the endosymbiont B. aphidicola is characterized by a genome size that is seven times smaller than E. coli (643 kb ...
Webb DC, Rosenberg H, Cox GB (1992). "Mutational analysis of the Escherichia coli phosphate-specific transport system, a member ... Saier MH Jr (1998). "Molecular phylogeny as a basis for the classification of transport proteins from bacteria, archaea and ... "Identification of a second Mycobacterium tuberculosis gene cluster encoding proteins of an ABC phosphate transporter". FEBS ...
... in vitro translation and Escherichia coli cloning studies". Proceedings of the National Academy of Sciences of the United ... Some viruses can encode proteins that bind to double-stranded RNA (dsRNA) to prevent the activity of RNA-dependent protein ... the E7 protein of Human papillomavirus (HPV), and the B18R protein of vaccinia virus. Reducing IFN-α activity may prevent ... phosphorylates ribosomal protein s6, which is involved in protein synthesis; and phosphorylates a translational repressor ...
December 1977). "Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin". Science. 198 ( ... Along with Stanley N. Cohen and Paul Berg he discovered a method to coax bacteria into producing foreign proteins, thereby jump ... By 1969, he performed studies on a couple of restriction enzymes of the E.coli bacterium with especially useful properties. He ...
... the TrfA protein, binds to and activates oriV. In Escherichia coli, replication proceeds unidirectionally from oriV after ... The IncP-1 plasmid group (IncP plasmids in Escherichia coli) of which RK2 is a part has been described as "highly potent, self- ... encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli ... 1997, Volume 63, Issue 2, p. 370 National Center for Biotechnology Information: "Escherichia coli W plasmid pRK2, complete ...
Singer P, Wu CW (October 1987). "Promoter search by Escherichia coli RNA polymerase on a circular DNA template". The Journal of ... A connector protein dimer (e.g. CTCF or YY1) stabilizes the loop by anchoring one member on the enhancer and the other on the ... Herbert M, Kolb A, Buc H (May 1986). "Overlapping promoters and their control in Escherichia coli: the gal case". Proceedings ... "Dynamics of transcription of closely spaced promoters in Escherichia coli, one event at a time". Journal of Theoretical Biology ...
For example, in E. coli, the formate:ferricytochrome-b1 oxidoreductase is an intrinsic membrane protein with two subunits and ... Graham A, Boxer DH (1981). "The organization of formate dehydrogenase in the cytoplasmic membrane of Escherichia coli". Biochem ... Ruiz-Herrera J, DeMoss JA (1969). "Nitrate reductase complex of Escherichia coli K-12: participation of specific formate ... Khangulov SV, Gladyshev VN, Dismukes GC, Stadtman TC (1998). "Selenium-Containing Formate Dehydrogenase H from Escherichia coli ...
He is known for his contributions in the fields of eye disease proteomics and mutagenic DNA repair in Escherichia coli. He is ... "Functional insights by comparison of modeled structures of 18kDa small heat shock protein and its mutant in Mycobacterium ... During his early researches on Escherichia coli, a gram-negative bacterium, he discovered the induction of mutagenic DNA repair ... tools and discovered the single nucleotide polymorphism in the unique small alpha crystalline like heat shock protein in the ...
... phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli". Mol ... This ncRNA was discovered in the bacteria Escherichia coli during a large scale computational screen for transcription signals ... 2001). "Novel small RNA-encoding genes in the intergenic regions of Escherichia coli". Curr. Biol. 11 (12): 941-950. doi: ... of the sRNA GlmZ in the activation of glmS expression and is subject to regulation by polyadenylation in Escherichia coli". ...
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR (November 2001). "An inner membrane enzyme in Salmonella and Escherichia coli ... "Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium". Protein Expression and ... "Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: ... polymyxin resistance protein PmrK, arnT (gene)) is an enzyme with systematic name 4-amino-4-deoxy-alpha-L-arabinopyranosyl ...
"The MerE protein encoded by transposon Tn21 is a broad mercury transporter in Escherichia coli". FEBS Letters. 583 (7): 1127- ... The MerC protein encoded on the IncJ plasmid pMERPH of the Shewanella putrefaciens mercuric resistance operon is 137 amino ... MerC proteins are homologous to other bacterial Hg2+ bacterial transporters. See Kiyono, Masako; Sone, Yuka; Nakamura, Ryosuke ... Wilson, J. R.; Leang, C.; Morby, A. P.; Hobman, J. L.; Brown, N. L. (2000-04-21). "MerF is a mercury transport protein: ...
Gunsalus RP (November 1992). "Control of electron flow in Escherichia coli: coordinated transcription of respiratory pathway ... In R. sphaeroides, DMSOR is a single-subunit, water-soluble protein that requires no additional cofactors beyond pterin. In E. ... coli, DMSOR is embedded within the membrane and has three unique subunits, one of which includes the characteristic pterin ... and DorC proteins. A study of lacZ fusions (reporter genes) to corresponding dorS, dorR, and dorC promoters concluded that ...
2008). "UpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli". J Bacteriol. ... Protein pages needing a picture, Protein families, Protein domains, Virulence factors, Gram-negative bacteria, Secretion, ... First, biogenesis of proteins in the Type V Secretion System (T5SS). Second, it is thought to target the protein to the inner ... However, the outer membrane is a barrier for the secretion of proteins, and it requires energy to transport proteins across the ...
Shearstone JR, Baneyx F (April 1999). "Biochemical characterization of the small heat shock protein IbpB from Escherichia coli ... "IbpA the small heat shock protein from Escherichia coli forms fibrils in the absence of its cochaperone IbpB". FEBS Letters. ... of the oligomeric state and chaperone-like activity for the polydisperse small heat shock protein IbpB from Escherichia coli". ... Richmond CS, Glasner JD, Mau R, Jin H, Blattner FR (October 1999). "Genome-wide expression profiling in Escherichia coli K-12 ...
"Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli". Proceedings of the National ...
The 245 nucleotide sRNA of Escherichia coli, CsrC, was discovered using a genetic screen for factors that regulate glycogen ... coli. CsrC antagonises the regulatory effects of CsrA, presumably by sequestering this protein. The discovery of CsrC is ... "Novel small RNA-encoding genes in the intergenic regions of Escherichia coli". Current Biology. 11 (12): 941-950. doi:10.1016/ ... "A novel sRNA component of the carbon storage regulatory system of Escherichia coli". Molecular Microbiology. 48 (3): 657-670. ...
Lesley et al used Escherichia coli to express all the open-reading frames (ORFs) of T. martima. These proteins were then ... Because protein structure is closely linked with protein function, the structural genomics has the potential to inform ... Experimental methods of protein structure determination require proteins that express and/or crystallize well, which may ... Protein Data Bank (PDB): repository for protein sequence and structural information UniProt: provides sequence and functional ...
"Expression of human cytochrome P450 46A1 in Escherichia coli: effects of N- and C-terminal modifications". Arch. Biochem. ... UniProt entry on Cholesterol-24 hydroxylase HMDB Database entry RCSB Protein Data Bank Entry Review on Cholesterol-24 ... Genetic cloning of the encoding gene (CYP46A1) was first accomplished in 1999 and an extensive E. coli expression and ... monomeric heme-containing protein bound to the endoplasmic reticulum in neurons. Cholesterol-24 hydroxylase is similar in ...
Escherichia coli, and Borrelia are not simply bags of biochemicals but instead program the locations of their protein ... G Laloux and C Jacobs-Wagner (2014) "How do bacteria localize proteins to the cell pole? J Cell Science 127: 11-19. doi:10.1242 ... She also discovered the protein crescentin which forms bacterial intermediate filaments, structures once thought to occur only ... and that proteins are directed by regulatory processes to locate to specific places within the bacterial cell. She was elected ...
Since then, the Escherichia coli IDH structure has been used by most researchers to make comparisons to other isocitrate ... Portal: Biology (Articles with short description, Short description matches Wikidata, Genes on human chromosome 2, Protein ... The Isocitrate Dehydrogenase (IDH) enzyme structure in Escherichia coli was the first IDH ortholog structure to be elucidated ... The dimer E. coli showed stability at a higher temperature than normal due to the interactions between the two monomeric ...
Escherichia coli, Salmonella, and Staphylococcus aureus are a few bacteria whose growth can be inhibited by alcohols. Alcohols ... The mode of action is by denaturing the proteins. Alcohols interfere with the hydrogen bonds present in the protein structure. ... to reduce the prevalence of Escherichia coli. Copper-alloy surfaces have natural intrinsic antimicrobial properties and can ... In the presence of water, 70% alcohol causes coagulation of the proteins thus inhibiting microbial growth. Alcohols are not ...
Denk D, Böck A (March 1987). "L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine ... In molecular biology, the protein domain SATase is short for Serine acetyltransferase and refers to an enzyme that catalyses ... Kredich NM, Tomkins GM (1966). "The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium". J. Biol. ... "The structure and mechanism of serine acetyltransferase from Escherichia coli". J. Biol. Chem. 279 (39): 40729-36. doi:10.1074/ ...
One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally unannotated (orphans). Many map to ... Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins PLoS Biol. 2009 Apr 28;7(4):e96. ... One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally unannotated (orphans). Many map to ... To elucidate the orphans biological roles, we performed an extensive proteomic survey using affinity-tagged E. coli strains ...
Purified fluorescent protein mCherry, with N-terminal HIS tag, expressed in E. coli, 250ug ... Purified fluorescent protein mCherry, with N-terminal HIS tag, expressed in E. coli, 250ug ... Video tutorial: Why protein expression host matters * Webinar Video: Overexpression lysate as positive controls for Western ... Protein Sequence (showhide) MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL ...
Solution structure of the C-terminal cytoplasmic domain residues 468-497 of Escherichia coli protein ProP ... Solution structure of the C-terminal cytoplasmic domain residues 468-497 of Escherichia coli protein ProP. *PDB DOI: 10.2210/ ... Transporter ProP of Escherichia coli, a proton symporter and a member of the major facilitator superfamily, senses osmotic ... Transporter ProP of Escherichia coli, a proton symporter and a member of the major facilitator superfamily, senses osmotic ...
Seminars and Events at the Research Institute of Molecular Pathology (IMP) and Vienna Biocenter (VBC).
Bacteria Escherichia coli. Reference. Maurizi MR. Proteases and protein degradation in Escherichia coli. Experientia. 1992 Feb ... Percent of of intracellular protein degradation dependent on metabolic energy. Range. ≤90 % ... An] intriguing and challenging property of intracellular protein degradation is its energy-dependence. As much as 90 % of ... intracellular protein degradation is dependent on metabolic energy, presumably supplied by ATP.. ...
Inhibition of human recombinant LSD1/CoREST protein expressed in Escherichia coli using monomethylated H3-K4 peptide as ...
Role of the rom protein in copy number control of plasmid pBR322 at different growth rates in Escherichia coli K-12. Plasmid 41 ... NusA protein of Escherichia coli is an efficient transcription termination factor for certain terminator sites. Journal of ... Effects of NusA protein on transcription termination in the tryptophan operon of Escherichia coli. Cell 29(3): 945-951, 1982 ... Escherichia coli rho factor Protein and enzyme of transcription termination. McKnight, S L , Yamamoto, K R Cold Spring Harbor ...
"Topographic analysis of the toxic Gef protein from Escherichia coli",. abstract = "The chromosomal gef gene of Escherichia coli ... Refn A, Molin S, Andersson P. Topographic analysis of the toxic Gef protein from Escherichia coli. Molecular Microbiology. 1991 ... Refn, A., Molin, S., & Andersson, P. (1991). Topographic analysis of the toxic Gef protein from Escherichia coli. Molecular ... Refn, A, Molin, S & Andersson, P 1991, Topographic analysis of the toxic Gef protein from Escherichia coli, Molecular ...
Structural evidence that colicin a protein binds to a novel binding site of TolA protein in Escherichia coli periplasm ... The Tol assembly of proteins is an interacting network of proteins located in the Escherichia coli cell envelope that ... Structural evidence that colicin a protein binds to a novel binding site of TolA protein in Escherichia coli periplasm. The ... Bacterial Toxins Microbiology Protein Structure Protein-Protein Interactions X-ray Crystallography Tol Bacteriocin Colicin. ...
Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein. / ... Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein. ... Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein. ... T1 - Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein ...
... are responsible for host diseases such as Neonatal Meningitis Escherichia coli (NMEC), the second-leading cause of neonatal ... Although OmpA is present in virtually all E. coli, differences in its amino acid residues have yet to be surveyed in ExPEC. ... Virulence factors associated with NMEC include outer membrane protein A (OmpA) and type I fimbriae (FimH), which also occur in ... The differences in OmpA protein sequences suggest that OmpA may influence variation in virulence and host specificity within ...
Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family. ... Protein Science, 2011, DOI: 10.1002/pro.594, 656-669 published on 24.01.2011. Protein Science, online article ... Further to the native protein, crystal structures were also solved for its variants D471N and D471E, which show functionally ...
The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart ... The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart ... The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart ... The RdgC protein of Escherichia coli binds DNA and counters a toxic effect of RecFOR in strains lacking the replication restart ...
... * QMRO Home ... Size Dependence of Protein Diffusion in the Cytoplasm of Escherichia coli. View/. Open. MULLINEAUXSizeDependence2010POSTP.pdf ( ...
Does single-stranded DNA pass through the inner channel of the protein hexamer in the complex with the Escherichia coli DnaB ... Does single-stranded DNA pass through the inner channel of the protein hexamer in the complex with the Escherichia coli DnaB ... Does single-stranded DNA pass through the inner channel of the protein hexamer in the complex with the Escherichia coli DnaB ... T1 - Does single-stranded DNA pass through the inner channel of the protein hexamer in the complex with the Escherichia coli ...
Colicin - immunity protein complexes are among the strongest protein complexes known. Here, the Usp associated immunity protein ... Our data infer that nonspecific DNA binding of the Imu3 immunity protein, prevents suicide of E. coli producing the genotoxin ... To prevent host suicide, colicins, bacteriocins of E. coli, form tight complexes with their cognate immunity proteins. ... Isolation and partial characterisation of the Usp-associated immunity protein-3 (Imu3) revealed that, while Usp and Imu3 do not ...
Cloning of the Gene Encoding DNA Binding Protein HU from Bacillus stearothermophilus and Its Expression in Escherichia coli. / ... Cloning of the Gene Encoding DNA Binding Protein HU from Bacillus stearothermophilus and Its Expression in Escherichia coli. In ... Cloning of the Gene Encoding DNA Binding Protein HU from Bacillus stearothermophilus and Its Expression in Escherichia coli. ... Cloning of the Gene Encoding DNA Binding Protein HU from Bacillus stearothermophilus and Its Expression in Escherichia coli. ...
... Annotation: b2097 orf, hypothetical protein (VIMSS) ... Hits to curated proteins without experimental data as to their function are never considered high confidence.) *HMMer finds a ... our ignorance of proteins functions, *omissions in the gene models, *frame-shift errors in the genome sequence, or *the ... GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can ...
The mutant studies in vivo reinforce the idea that the DNA binding activity is crucial for RdgCs function in Escherichia coli. ... In this study, I purified and crystallised the RdgC protein. The crystal structure of the protein was then revealed as a homo- ... RdgC was also found to regulate the activity of RecA, a key protein in recombination, both in vivo and in vitro. The function ... and I examined all the mutant proteins in DNA binding shift assays in vitro and in synthetic lethality assays in vivo. A DNA ...
Interrelationship of the phage lambda receptor protein and maltose transport in mutants of Escherichia coli K12. (English) ... Interrelationship of the phage lambda receptor protein and maltose transport in mutants of Escherichia coli K12.. scientific ...
Bacteria - Escherichia coli - Protein purification - GSK UK - 2005. by JackDanielHorner , Nov 17, 2021 ...
Trun, N. J., & Silhavy, T. J. (1989). The genetics of protein targeting in Escherichia coli K12. Journal of cell science, 93( ... Trun, N. J. ; Silhavy, T. J. / The genetics of protein targeting in Escherichia coli K12. In: Journal of cell science. 1989 ; ... The genetics of protein targeting in Escherichia coli K12. Journal of cell science. 1989 Jan 1;93(SUPPL. 11):13-28. doi: ... Trun, NJ & Silhavy, TJ 1989, The genetics of protein targeting in Escherichia coli K12, Journal of cell science, vol. 93, no ...
Recombinant Escherichia coli Type-1 fimbrial protein, A chain(fimA) ... Recombinant Escherichia coli Type-1 fimbrial protein, A chain(fimA). CSB-EP361210ENV Regular price $1,066.00 CAD ... Reference: "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."Burland V.D ... storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80 ...
Bicistronic Design-Based Continuous and High-Level Membrane Protein Production in Escherichia coli ... Bicistronic Design-Based Continuous and High-Level Membrane Protein Production in Escherichia coli Claassens, N. J., Finger-Bou ... 2019). Bicistronic Design-Based Continuous and High-Level Membrane Protein Production in Escherichia coli. ACS Synthetic ...
Analysis of the stimulation of DNA polymerase V of Escherichia coli by processivity proteins. ... Analysis of the stimulation of DNA polymerase V of Escherichia coli by processivity proteins. Maor-Shoshani A, Livneh Z ... Bypass of replication-blocking lesions in Escherichia coli is carried ... Bypass of replication-blocking lesions in Escherichia ... caused the same inhibition also in the presence of the processivity proteins. The in vivo role of the processivity proteins in ...
Dynamic Distribution of SeqA Protein across the Chromosome of Escherichia coli K-12. In: mBio. 2010 ; Vol. 1, No. 1. pp. e00012 ... Dynamic Distribution of SeqA Protein across the Chromosome of Escherichia coli K-12. mBio. 2010 May 18;1(1):e00012-10-e00012-10 ... Dynamic Distribution of SeqA Protein across the Chromosome of Escherichia coli K-12. / Sanchez-Romero, MA; Busby, Stephen; Dyer ... Dive into the research topics of Dynamic Distribution of SeqA Protein across the Chromosome of Escherichia coli K-12. ...
For nine proteins (L3, L4, L5, L7A, L10, L14, L19, L31, and L40), the molecular masses could not be determined. Proteins P1 and ... The 60S ribosomal proteins were isolated from ribosomes of human placenta and separated by reversed phase HPLC. The fractions ... Forty-six large subunit proteins were found, 22 of which showed masses in accordance with the SwissProt database (June 2002) ... A loss of methionine without acetylation was found for protein L8 and L17. ...
  • Genomewide transcriptional response of Escherichia coli O157:H7 to norepinephrine. (
  • Specifically, E coli serotype O157:H7 has been associated with more than 80% of infections leading to HUS. (
  • The possibility that E. coli O157:H7 was a contaminant in cookie dough surprised even the most experienced microbiologists here in CDC's Enteric Diseases Laboratory Branch. (
  • The road to last month's cookie dough recall started when CDC scientists reviewed information collected through PulseNet, a national network of laboratories that perform DNA "fingerprinting" of foodborne bacteria like E. coli O157:H7, Salmonella, and Listeria. (
  • The gene, hbst, was overexposed using the expression vector pET-5a in Escherichia coli, and the recombinant HU protein (r-BstHU) was purified to be homogeneity by heparin-agarose column chromatography followed by ion-exchange column chromatography on S-Sepharose. (
  • The recombinant protein thus obtained had a circular dichroism spectrum identical to that of the authentic protein and bound to DNA to the same extent as the authentic protein. (
  • Immobilization and utilization of the recombinant fusion proteins trypsin-streptavidin and streptavidin-transglutaminase for modification of whey protein isolate functionality. (
  • Buy RHD recombinant protein in Escherichia coli (E.coli) system at competitive price. (
  • Order RHD recombinant protein at ProteoGenix. (
  • Proteome profiling of the inclusion body (IB) fraction of recombinant proteins produced in Escherichia coli suggested that two small heat shock proteins, IbpA and IbpB, are the major proteins associated with IBs. (
  • In this study, we demonstrate that IbpA and IbpB facilitate the production of recombinant proteins in E. coli and play important roles in protecting recombinant proteins from degradation by cytoplasmic proteases. (
  • This strategy seems to be generally applicable as it was successfully employed for the enhanced cytosolic or secretory production of several other recombinant proteins in E. coli. (
  • A recombinant human EGF protein is produced by a technique called recombinase. (
  • Recombinant human EGF protein is a highly versatile protein used in diagnostics and target therapeutics. (
  • These results suggest that recombinant human EGF protein is a useful therapeutic tool in ophthalmology. (
  • These data suggest that recombinant human EGF protein may represent a new therapeutic target for gastric cancer treatment. (
  • The ibeA gene was subcloned into pET28a(+) and was expressed as a recombinant protein with an N-terminal histidine tag. (
  • The recombinant IbeA protein had much greater activity (50 times) in blocking the invasion of BMECs by Escherichia coli K1 than did the partial protein fragment, which provides further evidence that ibeA is an important determinant for E. coli K1 invasion of BMECs. (
  • However, high-level expression and purification of recombinant FGF21 (rFGF21) in Escherichia coli (E. coli) is difficult because rFGF21 forms inclusion bodies in the bacteria making it difficult to purify and obtain high concentrations of bioactive rFGF21. (
  • Using the pASK-IBA6 cloning vector, we produced recombinant terrelysin (rTerrelysin) as a fusion product in Escherichia coli. (
  • The recombinant protein was purified and using MALDI-TOF MS determined to have a mass of 16,428 Da. (
  • The microorganism Escherichia coli is usually used for recombinant protein manufacturing. (
  • A soluble multimeric recombinant CD2 protein identifies CD48 as a low affinity ligand for human CD2: divergence of CD2 ligands during the evolution of humans and mice. (
  • SEPX1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 136 amino acids (1-116 a.a.) and having a molecular mass of 14.8kDa. (
  • Many map to distant clades such as Archaea, suggesting involvement in basic prokaryotic traits, whereas others appear restricted to E. coli, including pathogenic strains. (
  • To elucidate the orphans' biological roles, we performed an extensive proteomic survey using affinity-tagged E. coli strains and generated comprehensive genomic context inferences to derive a high-confidence compendium for virtually the entire proteome consisting of 5,993 putative physical interactions and 74,776 putative functional associations, most of which are novel. (
  • OmpA contributes to NMEC's ability to cross the blood-brain barrier, persist in the bloodstream and has been identified as a potential vaccine target for ExPEC, however the protein has amino acid variants, which may influence virulence of strains or alter vaccine efficacy. (
  • We examined E. coli strains collected at our hospital to among the most frequently isolated bacterium in a variety determine the basis for resistance. (
  • Although cephamycin-resistant E. coli is relatively uncommon, widespread use of -lactam antiboties may Materials and Methods contribute to the development and spread of these strains. (
  • We collected strains of E. coli from midstream urine data from the Queen Elizabeth II Health Science Centre in from inpatients and from patients in the community. (
  • In both cases, we membrane proteins were differentially solubilized for 20 excluded duplicate strains from the same patient. (
  • A collection of E. coli strains isolated from human bacteremia was screened for the presence of aap, the dispersin-encoding gene. (
  • We examined the cytosolic production, and Tat- or Sec-dependent secretion of the enhanced green fluorescent protein (EGFP) in wild type, ibpAB - mutant, and ibpAB-amplified E. coli strains. (
  • Its production is a highly controlled process involving E. coli and other bacterial strains. (
  • The author highlights novel strains and methods that have recently been shown to express multidisulfide bonded proteins. (
  • FimA is the main structural subunit of adhesive type 1 pili from uropathogenic Escherichia coli strains. (
  • The α-pore-forming toxin Cytolysin A (ClyA) is responsible for the hemolytic activity of various Escherichia coli and Salmonella enterica strains. (
  • Ahmed MS, Islam MA, Hossain MM, Nasreen M. An In Silico Approach for Characterization of an Acetyltransfarase Protein from Shigella flexneriSerotype 5b (strain 8401). (
  • Furthermore, we show that Gef is anchored in the cytoplasmic membrane by the N-terminal part of the protein, and that the C-terminal part is localized in the periplasm in a dimeric form with at least one disulphide bond. (
  • The findings suggest the importance of phosphorylation of the ribosomal protein uS15 by cytoplasmic protein kinases at several sites for its efficient transfer into the nucleolus, where pre-ribosomal subunits are assembled. (
  • Subcellular localization predictions shows it is a cytoplasmic protein. (
  • Reference: Analysis of the stimulation of DNA polymerase V of Escherichia coli by processivity proteins. (
  • Bypass of replication-blocking lesions in Escherichia coli is carried out by DNA polymerase V (UmuC) in a reaction that requires UmuD', RecA, and single-strand DNA-binding protein (SSB). (
  • To overcome this problem, we fused the FGF21 with SUMO (Small ubiquitin-related modifier) by polymerase chain reaction (PCR), and expressed the fused gene in E. coli BL21(DE3). (
  • Mass spectrometry of Escherichia coli RNA polymerase: interactions of the core enzyme with sigma70 and Rsd protein. (
  • The E. coli RNA polymerase core enzyme is a multisubunit complex of 388,981 Da. (
  • Polynucleotide phosphorylase functions both as a 3′→ 5′ exonuclease and a poly (A) polymerase in Escherichia coli. (
  • Analysis of the function of Escherichia coli poly (A) polymerase I in RNA metabolism. (
  • Reference: DNA polymerase III accessory proteins. (
  • Genes encoding the chi and psi accessory proteins of the DNA polymerase III holoenzyme replicase of Escherichia coli have been identified, sequenced, and used to express and purify both chi and psi in quantity. (
  • One-third of the 4,225 protein-coding genes of Escherichia coli K-12 remain functionally unannotated (orphans). (
  • Proteomic LC-MS analysis of Arabidopsis cytosolic ribosomes: Identification of ribosomal protein paralogs and re-annotation of the ribosomal protein genes. (
  • Diarrheagenic E. coli have thus used the histone-like structuring protein, H-NS, which binds and silences AT-rich genes. (
  • Exactly how H-NS is harnessed, and more broadly, how these pathogens both regulate cross-talk between acquired genes and the core E. coli genome as well as control the switch between nonpathogenic and pathogenic lifestyles, are subjects of intense and fascinating investigation. (
  • Enteroaggregative Escherichia coli (EAEC), a common diarrheagenic E. coli pathotype, utilizes a member of the AraC family of transcriptional regulators, AggR, to regulate the expression of virulence genes which are necessary for host colonization and virulence. (
  • In summary, my work extends the characterization of a novel, but common, regulatory protein that regulates both virulence and core metabolic genes, and provides new insights into pathogenic gene regulation. (
  • Altogether, 32 genes encoding hypothetical proteins of unknown function (HPUFs) were identified from the genomic sequence of fHy-Eco03. (
  • Although, providing the massive amount of data by recent genome sequencing projects but many of these genomes are still not fully annotated as well as consist of genes/proteins with unknown function and structure. (
  • Most patients with atypical HUS have mutations in one or more of the genes that encode proteins involved in the alternate pathway of complement, which creates a predisposition to the disorder. (
  • I know it is summer when a quick review of our foodborne outbreak watch board shows four multistate outbreak investigations: Salmonella Chester, Salmonella Baildon, Salmonella Hartford, and E. coli O157 due to contaminated bison meat products. (
  • GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (
  • Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. (
  • However, the genome-wide DNA binding properties of SeqA are unknown, and hence, here, we describe a study of the binding of SeqA across the entire Escherichia coli K-12 chromosome, using chromatin immunoprecipitation in combination with DNA microarrays. (
  • Complete genome sequence of Escherichia coli Antibiotic-Resistance Isolate AR Bank #0346. (
  • Membrane proteins roughly constitute 30% of open reading frames in a genome and form 70% of current drug targets. (
  • In Escherichia coli, this RNP complex is composed of a catalytic RNA subunit, M1 RNA, and a protein cofactor, C5 protein. (
  • The structure of the regulatory subunit of the protein kinase CK2 crystallized in the presence of p21 WAF1 suggests binding in the solvent-accessible part of the zinc-finger motif. (
  • protein_coding" "AAC73969","clpA","Escherichia coli","ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity [Ensembl]. (
  • The lungs were sliced into 5 micrometer thick sections which were examined for p65, a protein subunit of NF-kB, using an immunofluorescence technique. (
  • Mutants in Escherichia coli transcription termination factor Rho, termed rho(nusD), were previously isolated based on their ability to block the growth of bacteriophage T4. (
  • Decreased E. coli message lifetimes could be because of increased ribonuclease activity in the rho mutant cells: if a Rho-dependent terminator precedes a ribonuclease gene, weaker termination in the rho mutants could lead to nuclease overexpression. (
  • Interrelationship of the phage lambda receptor protein and maltose transport in mutants of Escherichia coli K12. (
  • T ations may protect E. coli and allow subsequent selection he development of antibiotic resistance in Escherichia for promoter and attenuator mutants. (
  • Unexpectedly, expression of the pBR322 Rop protein, a structure-specific, sequence-independent RNA-binding protein, in rho(nusD) cells restored the ability of T4 to grow and prolonged cellular message half-life in both the wild-type and the rho026 mutant. (
  • The deduced amino acid sequence of an open reading frame was perfectly matched with that of B. stearothermophilus HU (BstHU) determined by the protein chemical methods [M. Kimura and K. S. Wilson, J. Biol. (
  • It does not share significant sequence homology with other subtypes of small G-protein GEF motifs such as the Cdc25 domain and the Sec7 domain, which specifically interact with Ras and ARF family small GTPases, respectively, nor with other Rho protein interactive motifs, indicating that the Dbl family proteins are evolutionarily unique. (
  • The specific sequence of amino acids determines the shape the polypeptide will take, during protein folding, and the function of the protein. (
  • Sequence similarity was brought in through Protein Data Bank and non-redundant database using BLASTp program of NCBI and a search for templates revealed that yjaB shares 97% homology to a protein of Escherichia coli, indicating this protein is evolutionary conserved and was found with acetyltransfarase. (
  • Rat CT-1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1. (
  • The chromosomal gef gene of Escherichia coli is a member of the gef gene family which encodes strongly toxic proteins of about 50 amino acids. (
  • The OmpA protein forms four extracellular loops that exhibit residue patterns encoded by allelic variants in the ompA gene across the protein's loops [ 13 ]. (
  • The gene (hbst) encoding the DNA binding protein HU from Bacillus stearothermophilus was cloned, with the Bacillus subtilis HU gene (hbsu) as a hybridization probe. (
  • In silico digestion of all 409 ribosomal protein sequences in Arabidopsis defined the proportion of theoretical gene-specific peptides for each gene family and highlighted the need for low m/z cutoffs of MS ion selection for MS/MS to characterize low molecular weight, highly basic ribosome proteins. (
  • To bolster their findings, they then expressed one gene from each superfamily in Escherichia coli and purified the protein. (
  • Expression in Escherichia coli of cDNA fragments encoding the gene for the host-protective antigen of infectious bursal disease virus. (
  • 6980 /gene="ORF2" /function="structural" /note="ORF2 encodes a capsid protein which makes nucleocapsid. (
  • Dispersin is a 10.2 kDa-immunogenic protein secreted by enteroaggregative Escherichia coli (EAEC). (
  • The interface region of the TA53-107-TolAIII complex consists of polar contacts linking residues Arg-92 to Arg-96 of ColA with residues Leu-375-Pro-380 of TolA, which constitutes a β-strand addition commonly seen in more promiscuous protein-protein contacts. (
  • Although OmpA is present in virtually all E. coli , differences in its amino acid residues have yet to be surveyed in ExPEC. (
  • The results from this EPR spectroscopy-based approach together with thOSe from earlier studies identify residues in C5 protein which are proximal to M1 RNA in the RNase P holoenzyme complex. (
  • Binding to plasminogen was also investigated due to the presence of conserved carboxy-terminal lysine residues in dispersin sequences, which are involved in plasminogen binding in several bacterial proteins. (
  • Nevertheless, the set of residues still imposes limitations on potential protein applications. (
  • The proteins encoded by members of the Dbl family share a common domain, presented in this entry, of about 200 residues (designated the Dbl homology or DH domain) that has been shown to encode a GEF activity specific for a number of Rho family members. (
  • A hypothetical protein yjaB of these bacteria, consisting of 147 residues was picked out for in silico analysis. (
  • The candidate cysteines are part of a motif that is conserved in the RNase E protein family, and mutation of these residues causes the partial loss of zinc, the complete disruption of the tetramer into dimers, and effective catalytic inactivation. (
  • Crafting these residues, which are located in loop regions between TPR motifs, onto the monomeric consensus TPR protein CTPR3 induced the formation of oligomers. (
  • Proteins are large biomolecules consisting of one or more long chains of amino acid residues. (
  • TA53-107 binds on the opposite side of TolAIII to that used by g3p, ColN, or TolB, illustrating the flexible nature of TolA as a periplasmic hub protein. (
  • We show that the nucleoid-associated RdgC protein binds non-specifically to single-stranded (ss) DNA and double-stranded DNA. (
  • The bacterial SeqA protein binds to hemi-methylated GATC sequences that arise in newly synthesized DNA upon passage of the replication machinery. (
  • In Escherichia coli K-12, the single replication origin oriC is a well-characterized target for SeqA, which binds to multiple hemi-methylated GATC sequences immediately after replication has initiated. (
  • In this study, we describe how we have been able to identify scores of targets, across the entire Escherichia coli chromosome, to which SeqA binds. (
  • In addition to controlling/promoting expression of virulence factors, AggR also regulates the expression of its own negative regulator, aar, which encodes a small protein that binds in vitro to the dimerization site of AggR and thus blocks AggR function. (
  • The regulator of sigma70, Rsd protein, has previously been identified as a protein that binds to free sigma70. (
  • It binds to CD244 1 , with low affinity to CD2 2 , E. coli FimH 3 and heparan sulfate. (
  • Identification of Eggshell Membrane Proteins and Purification of Ovotransferrin and β-NAGase from Hen Egg White. (
  • Purification and characterization of the fusion protein trypsin-streptavidin expressed in Escherichia coli. (
  • Biodefense properties of milk: The role of antimicrobial proteins and peptides. (
  • In the secretion of the Phyllomedusa hypocondrialis , they identified peptides (protein fragments) capable of eliminating bacteria that cause diarrhea or hospital infections and even bring blood pressure down. (
  • In addition, mutation or altered and OmpF porin proteins, and 1 showed decreased pro- expression of outer membrane proteins constituting porins duction of both. (
  • Virulence factors associated with NMEC include outer membrane protein A (OmpA) and type I fimbriae (FimH), which also occur in APEC and UPEC. (
  • Membrane binding of Escherichia coli RNaseE catalytic domain stabilizes protein structure and increases RNA substrate affinity. (
  • They are classified as integral, peripheral membrane proteins and polypeptide toxins. (
  • it encodes a 147-amino acid protein of 16.6 kDa. (
  • holD encoding psi lies upstream of rimI at 99.3 min and encodes a 137-amino acid protein of 15.2 kDa. (
  • We provide additional experimental evidence supporting orphan participation in protein synthesis, amino acid metabolism, biofilm formation, motility, and assembly of the bacterial cell envelope. (
  • The canonical set of amino acids leads to an exceptionally wide range of protein functionality. (
  • This genetic code expansion enables the incorporation of a noncanonical amino acid at a single, given site within the protein. (
  • The translation machinery accepts the noncanonical amino acid as a surrogate to incorporate it at canonically prescribed locations, i.e., all occurrences of a canonical amino acid in the protein are replaced by the noncanonical one. (
  • The incorporation of noncanonical amino acids can change the protein structure, causing considerably modified physical and chemical properties. (
  • Noncanonical amino acid analogs often act as cell growth inhibitors for expression hosts since they modify endogenous proteins, limiting in vivo protein production. (
  • In vivo incorporation of toxic noncanonical amino acids into proteins remains particularly challenging. (
  • In this study, the DNA encoding sequences of Cpf1 nuclease fragment (N-terminal 166 amino acids) were subcloned into pET-28a (+) vector and expressed in E. coli BL21. (
  • Typical HUS is related to bacteria, with more than 90% following a gastrointestinal infection with Shiga toxin-producing Escherichia coli (STEC). (
  • We have shown earlier that the highly conserved catalytic domain of E. coli RNase E is a homotetramer [Callaghan, A. J. et al. (
  • This event is prevented by small Heat Shock Proteins (sHSPs) acting as molecular chaperones. (
  • Small heat shock proteins (sHSPs) are a ubiquitous family of molecular chaperones that play a vital role in maintaining protein homeostasis in cells. (
  • Proteomic characterization of archaeal ribosomes reveals the presence of novel archaeal-specific ribosomal proteins. (
  • Savery, NJ , Rhodius, V & Busby, S 1996, ' Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein ', Philosophical Transactions B: Biological Sciences , vol. 351, pp. 543 - 550. (
  • The guanine nucleotide exchange factor (GEF) Dbl targets Rho family proteins thereby stimulating their GDP/GTP exchange, and thus is believed to be involved in receptor-mediated regulation of the proteins. (
  • Ribonuclease P processes polycistronic tRNA transcripts in Escherichia coli independent of ribonuclease E. (
  • Ribonuclease E is an essential hydrolytic endonuclease in Escherichia coli, and it plays a central role in maintaining the balance and composition of the messenger RNA population. (
  • Here, we report on functional in vitro and in vivo analyses of seven resurrected Precambrian thioredoxins, dating back 1-4 billion years, in the Escherichia coli cytoplasm. (
  • PriA protein provides a means to load the DnaB replicative helicase at DNA replication fork and D loop structures, and is therefore a key factor in the rescue of stalled or broken forks and subsequent replication restart. (
  • The structure of the complex of the Escherichia coli primary replicative helicase DnaB protein with single-stranded (ss) DNA and replication fork substrates has been examined using the fluorescence energy transfer method. (
  • It has been shown that OmpA can exist as a monomer or dimer and the soluble C-terminal domain of OmpA is responsible for protein dimerization [ 12 ]. (
  • This thesis is focused on the function of hypothetical protein Spr1057 of Streptococcus pneumoniae with an unknown function. (
  • The hypothetical protein yjaB shows acetyltransfarase activity. (
  • Its activity is directed by intracellular signals mediated by various types of receptors such as G protein-coupled receptors. (
  • Genomic analysis in Escherichia coli demonstrates differential roles for polynucleotide phosphorylase and RNase II in mRNA abundance and decay. (
  • Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus. (
  • Association of the proto-oncogene product dbl with G protein betagamma subunits. (
  • Proteins Proteins Linear polypeptides that are synthesized on ribosomes and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. (
  • Lck 5 , fyn 5 , LAT 6 and G protein subunits. (
  • The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes. (
  • In these experiments, we used the DnaB protein variant, R14C, which has arginine 14 replaced by cysteine in the small 12-kDa domain of the protein using site-directed mutagenesis. (
  • The pK a s of the two cysteine thiols in the Escherichia coli protein are removed from the expected value of 8.4 by ~1 pH unit in either direction, upward and downward. (
  • Two protomers share a single zinc ion, and quantitative analysis indicates that each protein contributes two cysteine thiols toward the coordination of the metal. (
  • Because phosphorus is an essential element for DNA, RNA, several ubiquitous cofactors, and phosphorylated proteins, Pi-limitation has major affects on cellular metabolism and physiology. (
  • Members of the Extraintestinal Pathogenic Escherichia coli (ExPEC) pathotype are adapted for an extraintestinal lifestyle. (
  • I work in CDC's Enteric Diseases Epidemiology Branch and we just spent a few busy weeks on an investigation linking E. coli 0157 illnesses to raw cookie dough. (
  • Extraintestinal Pathogenic E. coli (ExPEC), are responsible for host diseases such as Neonatal Meningitis Escherichia coli (NMEC), the second-leading cause of neonatal bacterial meningitis, Avian Pathogenic E. coli (APEC), a cause of extraintestinal disease in poultry, and Uropathogenic E. coli (UPEC), the most common cause of urinary tract infections. (
  • Does single-stranded DNA pass through the inner channel of the protein hexamer in the complex with the Escherichia coli DnaB helicase? (
  • The in vivo role of the processivity proteins in translesion replication was examined by assaying UV mutagenesis. (
  • METTL18 is established as the second human histidine-specific protein MTase, and its functional relevance is demonstrated, indicating that METTL18-mediated methylation of RPL3 is important for optimal ribosome biogenesis and function. (
  • Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution. (
  • Tyrosine kinase enzymes are responsible for activating many proteins by signal transduction cascades, phosphorylation, and other mechanisms. (
  • The DNA-binding domain of human c-Abl tyrosine kinase promotes the interaction of a HMG chromosomal protein with DNA. (
  • Like all members of the Ras superfamily, the Rho proteins cycle between active GTP-bound and inactive GDP-bound conformational states. (
  • 2B4, the natural killer and T cell immunoglobulin superfamily surface protein, is a ligand for CD48. (
  • dnaC suppressors of priA overcome this inviability, especially when RecF, RecO or RecR is inactivated, indicating that RdgC avoids or counters a toxic effect of these proteins. (
  • Mutations modifying ssDNA-binding (SSB) protein also negate this toxic effect, suggesting that the toxicity reflects inappropriate loading of RecA on SSB-coated ssDNA, leading to excessive or untimely RecA activity. (
  • It is a transparent viscous jelly that protects the Phyllomedusa hypocondrialis from dehydration and makes an indigestible meal for its predators, for containing a mixture of toxic proteins. (
  • Maurizi MR. Proteases and protein degradation in Escherichia coli. (
  • An] intriguing and challenging property of intracellular protein degradation is its energy-dependence. (
  • As much as 90 % of intracellular protein degradation is dependent on metabolic energy, presumably supplied by ATP. (
  • These observations provide a rationale for the mediation of active site pH control, an important aspect of the mechanism of thioredoxin and other proteins with catalytic thioredoxin domains, such as protein disulfide isomerases. (
  • The inability of the RNA-binding proteins SrmB and DeaD to reverse the rho mutant phenotype when each is overexpressed implies that the required RNA interactions are specific. (
  • The interface region also includes three cation-π interactions (Tyr-58-Lys-368, Tyr-90-Lys-379, Phe-94-Lys-396), which have not been observed in any other colicin-Tol protein complex. (
  • Initially characterized in EAEC, dispersin has been detected in other E. coli pathotypes, including those isolated from extraintestinal sites. (