Ability of ERYTHROCYTES to change shape as they pass through narrow spaces, such as the microvasculature.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
The internal resistance of the BLOOD to shear forces. The in vitro measure of whole blood viscosity is of limited clinical utility because it bears little relationship to the actual viscosity within the circulation, but an increase in the viscosity of circulating blood can contribute to morbidity in patients suffering from disorders such as SICKLE CELL ANEMIA and POLYCYTHEMIA.
The formation of clumps of RED BLOOD CELLS under low or non-flow conditions, resulting from the attraction forces between the red blood cells. The cells adhere to each other in rouleaux aggregates. Slight mechanical force, such as occurs in the circulation, is enough to disperse these aggregates. Stronger or weaker than normal aggregation may result from a variety of effects in the ERYTHROCYTE MEMBRANE or in BLOOD PLASMA. The degree of aggregation is affected by ERYTHROCYTE DEFORMABILITY, erythrocyte membrane sialylation, masking of negative surface charge by plasma proteins, etc. BLOOD VISCOSITY and the ERYTHROCYTE SEDIMENTATION RATE are affected by the amount of erythrocyte aggregation and are parameters used to measure the aggregation.
The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.
Venoms produced by the wasp (Vespid) family of stinging insects, including hornets; the venoms contain enzymes, biogenic amines, histamine releasing factors, kinins, toxic polypeptides, etc., and are similar to bee venoms.
The senescence of RED BLOOD CELLS. Lacking the organelles that make protein synthesis possible, the mature erythrocyte is incapable of self-repair, reproduction, and carrying out certain functions performed by other cells. This limits the average life span of an erythrocyte to 120 days.
The semi-permeable outer structure of a red blood cell. It is known as a red cell 'ghost' after HEMOLYSIS.
The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY.
RED BLOOD CELL sensitivity to change in OSMOTIC PRESSURE. When exposed to a hypotonic concentration of sodium in a solution, red cells take in more water, swell until the capacity of the cell membrane is exceeded, and burst.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
Persistently high systemic arterial BLOOD PRESSURE. Based on multiple readings (BLOOD PRESSURE DETERMINATION), hypertension is currently defined as when SYSTOLIC PRESSURE is consistently greater than 140 mm Hg or when DIASTOLIC PRESSURE is consistently 90 mm Hg or more.
A trace element with the atomic symbol Ni, atomic number 28, and atomic weight 58.69. It is a cofactor of the enzyme UREASE.
Forced expiratory effort against a closed GLOTTIS.
A type of stress exerted uniformly in all directions. Its measure is the force exerted per unit area. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Dissertations embodying results of original research and especially substantiating a specific view, e.g., substantial papers written by candidates for an academic degree under the individual direction of a professor or papers written by undergraduates desirous of achieving honors or distinction.
Glycosides from plants of the genus DIGITALIS. Some of these are useful as cardiotonic and anti-arrhythmia agents. Included also are semi-synthetic derivatives of the naturally occurring glycosides. The term has sometimes been used more broadly to include all CARDIAC GLYCOSIDES, but here is restricted to those related to Digitalis.
A genus of toxic herbaceous Eurasian plants of the Plantaginaceae which yield cardiotonic DIGITALIS GLYCOSIDES. The most useful species are Digitalis lanata and D. purpurea.
The resistance that a gaseous or liquid system offers to flow when it is subjected to shear stress. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
The teaching staff and members of the administrative staff having academic rank in a medical school.
The teaching staff and members of the administrative staff having academic rank in an educational institution.
The teaching staff and members of the administrative staff having academic rank in a dental school.
The teaching staff and members of the administrative staff having academic rank in a nursing school.

Calculation of a Gap restoration in the membrane skeleton of the red blood cell: possible role for myosin II in local repair. (1/352)

Human red blood cells contain all of the elements involved in the formation of nonmuscle actomyosin II complexes (V. M. Fowler. 1986. J. Cell. Biochem. 31:1-9; 1996. Curr. Opin. Cell Biol. 8:86-96). No clear function has yet been attributed to these complexes. Using a mathematical model for the structure of the red blood cell spectrin skeleton (M. J. Saxton. 1992. J. Theor. Biol. 155:517-536), we have explored a possible role for myosin II bipolar minifilaments in the restoration of the membrane skeleton, which may be locally damaged by major mechanical or chemical stress. We propose that the establishment of stable links between distant antiparallel actin protofilaments after a local myosin II activation may initiate the repair of the disrupted area. We show that it is possible to define conditions in which the calculated number of myosin II minifilaments bound to actin protofilaments is consistent with the estimated number of myosin II minifilaments present in the red blood cells. A clear restoration effect can be observed when more than 50% of the spectrin polymers of a defined area are disrupted. It corresponds to a significant increase in the spectrin density in the protein free region of the membrane. This may be involved in a more complex repair process of the red blood cell membrane, which includes the vesiculation of the bilayer and the compaction of the disassembled spectrin network.  (+info)

Structural and functional consequences of antigenic modulation of red blood cells with methoxypoly(ethylene glycol). (2/352)

We previously showed that the covalent modification of the red blood cell (RBC) surface with methoxypoly(ethylene glycol) [mPEG; MW approximately 5 kD] could significantly attenuate the immunologic recognition of surface antigens. However, to make these antigenically silent RBC a clinically viable option, the mPEG-modified RBC must maintain normal cellular structure and functions. To this end, mPEG-derivatization was found to have no significant detrimental effects on RBC structure or function at concentrations that effectively blocked antigenic recognition of a variety of RBC antigens. Importantly, RBC lysis, morphology, and hemoglobin oxidation state were unaffected by mPEG-modification. Furthermore, as shown by functional studies of Band 3, a major site of modification, PEG-binding does not affect protein function, as evidenced by normal SO4- flux. Similarly, Na+ and K+ homeostasis were unaffected. The functional aspects of the mPEG-modified RBC were also maintained, as evidenced by normal oxygen binding and cellular deformability. Perhaps most importantly, mPEG-derivatized mouse RBC showed normal in vivo survival ( approximately 50 days) with no sensitization after repeated transfusions. These data further support the hypothesis that the covalent attachment of nonimmunogenic materials (eg, mPEG) to intact RBC may have significant application in transfusion medicine, especially for the chronically transfused and/or allosensitized patient.  (+info)

Stomatocytosis is absent in "stomatin"-deficient murine red blood cells. (3/352)

To examine the relationship between erythrocyte membrane protein 7. 2b deficiency and the hemolytic anemia of human hereditary stomatocytosis, we created 7.2b knock-out mice by standard gene targeting approaches. Immunoblots showed that homozygous knock-out mice completely lacked erythrocyte protein 7.2b. Despite the absence of protein 7.2b, there was no hemolytic anemia and mouse red blood cells (RBCs) were normal in morphology, cell indices, hydration status, monovalent cation content, and ability to translocate lipids. The absence of the phenotype of hereditary stomatocytosis implies that protein 7.2b deficiency plays no direct role in the etiology of this disorder and casts doubt on the previously proposed role of this protein as a mediator of cation transport in RBC.  (+info)

Beta-adrenergic agonists regulate cell membrane fluctuations of human erythrocytes. (4/352)

1. Mechanical fluctuations of the cell membrane (CMFs) in human erythrocytes reflect the bending deformability of the membrane-skeleton complex. These fluctuations were monitored by time-dependent light scattering from a small area ( approximately 0. 25 microm2) of the cell surface by a method based on point dark field microscopy. 2. Exposure of red blood cells (RBCs) to adrenaline (epinephrine) and isoproterenol (isoprenaline) resulted in up to a 45 % increase in the maximal fluctuation amplitude and up to a 35 % increase in the half-width of the amplitude distribution. The power spectra of membrane fluctuations of control and treated cells revealed that adrenaline stimulated only the low frequency component (0.3-3 Hz). Analysis of the dose-response curves of beta-adrenergic agonists yielded an EC50 of 5 x 10-9 and 1 x 10-11 M for adrenaline and isoproterenol, respectively. Propranolol had an inhibitory effect on the stimulatory effect of isoproterenol. These findings show a potency order of propranolol > isoproterenol > adrenaline. 3. The stimulatory effect of adrenaline was a temporal one, reaching its maximal level after 20-30 min but being abolished after 60 min. However, in the presence of 3-isobutyl-1-methylxanthine, a partial stimulatory effect was maintained even after 60 min. Pentoxifylline and 8-bromo-cAMP elevated CMFs. However, exposure of ATP-depleted erythrocytes to adrenaline or 8-bromo-cAMP did not yield any elevation in CMFs. These findings suggest that the beta-agonist effect on CMFs is transduced via a cAMP-dependent pathway. 4. Deoxygenation decreased CMFs and filterability of erythrocytes by approximately 30 %. The stimulatory effect of isoproterenol on CMFs was 2.2-fold higher in deoxygenated RBCs than in oxygenated cells. 5. Exposure of RBCs to adrenaline resulted in a concentration-dependent increase in RBC filterability, demonstrating a linear relationship between CMFs and filterability, under the same exposure conditions to adrenaline. These findings suggest that beta-adrenergic agonists may improve passage of erythrocytes through microvasculature, enhancing oxygen delivery to tissues, especially under situations of reduced oxygen tension for periods longer than 20 min.  (+info)

Nisoldipine improves the impaired erythrocyte deformability correlating with elevated intracellular free calcium-ion concentration and poor glycaemic control in NIDDM. (5/352)

AIMS: To explore the mechanisms underlying the impaired erythrocyte deformability (RBC-df) in diabetic patients, the relationship between erythrocyte intracellular free calcium-ion concentration ([Ca2+]i) and RBC-df, and the effects of Ca2+-channel blocker on [Ca2+]i and RBC-df were evaluated. METHODS: Forty-eight patients with NIDDM and 24 control subjects were enrolled in this study. [Ca2+]i was determined using fura-2, and RBC-df by filtration method expressed as Deformability Index (DI). Erythrocytes were treated with nisoldipine to evaluate the effects of a Ca2+-channel blocker. RESULTS: [Ca2+]i was significantly higher (82.6 (78.0-87.2) vs 76.6 (74.3-81.2) nmol lRBC-1, P<0.001), and DI was significantly lower (0. 14 (0.09-0.28) vs 0.22 (0.16-0.28), P<0.01) in NIDDM than in controls. There was a significant correlation between HbA1c and [Ca2+]i (r=0.38, P<0.01), between HbA1c and DI (r=-0.51, P<0.01), and between [Ca2+]i and DI (r=-0.42, P<0.01). Stepwise multiple regression analysis revealed HbA1c and [Ca2+]i as independent determinants for the impaired RBC-df. Nisoldipine treatment in vitro significantly decreased [Ca2+]i, and significantly improved RBC-df. CONCLUSIONS: These data indicate that the impaired RBC-df in NIDDM may at least partly be attributed to the elevated [Ca2+]i and poor glycaemic control. In addition, favorable effects of a Ca2+-channel blocker on both [Ca2+]i and RBC-df have been demonstrated.  (+info)

Red blood cell deformability as a predictor of anemia in severe falciparum malaria. (6/352)

Decreased erythropoiesis and increased clearance of both parasitized and noninfected erythrocytes both contribute to the pathogenesis of anemia in falciparum malaria. Erythrocytes with reduced deformability are more likely to be cleared from the circulation by the spleen, a process that is augmented in acute malaria. Using a laser diffraction technique, we measured red blood cell (RBC) deformability over a range of shear stresses and related this to the severity of anemia in 36 adults with severe falciparum malaria. The RBC deformability at a high shear stress of 30 Pa, similar to that encountered in the splenic sinusoids, showed a significant positive correlation with the nadir in hemoglobin concentration during hospitalization (r = 0.49, P < 0.002). Exclusion of five patients with microcytic anemia strengthened this relationship (r = 0.64, P < 0.001). Reduction in RBC deformability resulted mainly from changes in unparasitized erythrocytes. Reduced deformability of uninfected erythrocytes at high shear stresses and subsequent splenic removal of these cells may be an important contributor to the anemia of severe malaria.  (+info)

Greater erythrocyte deformability in world-class endurance athletes. (7/352)

Because athletes during endurance events require rapid uptake of oxygen, the ability of red blood cells (RBC) to move through capillaries may limit performance. Using ektacytometry, we determined whether RBC deformability (RCD) differed between elite road cyclists (n = 9) and sedentary controls (n = 5). Density profiles and standard hematological measurements were also performed. The deformability index (DI) was higher in the cyclists (0.723 +/- 0.027) compared with that in controls (0.619 +/- 0.040, P < 0.001). Cyclists also had a larger percentage of low-density RBCs (P < 0. 001), and mean cell volume (MCV) was also higher (P = 0.013). These findings are indicative of a larger proportion of "young" RBCs in the blood of elite cyclists and provide further evidence that the turnover of RBCs in endurance athletes is higher than in the general population. With a younger more deformable RBC population and providing the destruction does not exceed replacement, performance potential should be enhanced. Furthermore, examination of factors that contribute to increased RBC turnover in athletes may help us understand the mechanisms that cause RBC aging.  (+info)

Hemorheology and walking of peripheral arterial occlusive diseases patients during treatment with Ginkgo biloba extract. (8/352)

AIM: To study the effects of Ginkgo biloba extract 761 (GbE) from the points of view of hemorheology for patients of peripheral arterial occlusive diseases (PAOD). METHODS: The treatment with GbE (240 mg.d-1, po) and the pain-free walking distance (PFWD) were carried out for 24 PAOD patients (12 nondiabetic, ND and 12 diabetic, D) over 48 wk. The parameters erythrocyte stiffness (ES) and relaxation time (RT), the blood plasma viscosity (eta), the plasma fibrinogen concentration (Cf) and the blood sedimentation rate (BSR), the PFWD, and maximal walking distance (MWD) were determined at 6 wk before treatment (-6), at the beginning of the treatment (0), and after 6, 11, 16, and 48 wk of treatment. RESULTS: At wk -6, ES and RT of both the ND- and D-group were not significantly different from a healthy control group. At wk 0, stiffness and RT were significantly higher than healthy control, and the mean PFWD was only 111 m. The eta value was significantly elevated and Cf and BSR were enhanced. Throughout 11 wk of treatment ES, RT, eta, and Cf decreased gradually and PFWD improved. Between 16 and 48 wk, ES, and RT were no longer significantly different from the controls, whereas eta and Cf decreased gradually but remained higher than normal, BSR decreased, and the PFWD improved by a factor of 3.8 times (D) and 3.3 times (ND). CONCLUSION: GbE gives therapeutic effects in PAOD patients.  (+info)

TY - JOUR. T1 - Erythrocyte deformability and its hemorheological consideration. AU - Shin, Sehyun. AU - Ku, Yunhee. AU - Park, Myung Su. AU - Suh, Jang Soo. PY - 2004/12/1. Y1 - 2004/12/1. N2 - The suspension of hardened erythrocytes (red blood cells) differs from the suspension of normal erythrocytes with respect to their rheological behavior. The deformability of normal and hardened erythrocytes (obtained by heating blood at 49°C or by incubating erythrocytes in a solution of hydrogen peroxide) was measured with a slit diffractometer and erythrocyte suspension viscosity was measured with a rotational viscometer. We found that when erythrocytes were heated at 49°C, with much less deformability than normal erythrocytes, their suspension viscosity was almost the same as the normal blood viscosity at high shear rates, whereas when erythrocytes were incubated in a solution of hydrogen peroxide, with an intermediate decrease of deformability, their suspension viscosity was greatly increased. The ...
The hemorheological profile in multiple myeloma (MM) has been extensively studied. Our investigation regarded the behavior of whole-blood viscosity, plasma viscosity and erythrocyte deformability in MM. We enrolled 24 MM patients; 13 of them had been
[Erythrocyte deformability: physiological aspects].: Analysis of published and authors data on the physiological role of deformability of erythrocytes, general
In this study, we investigated the effect of ginkgoglycoside in two different doses (19.2 mg/day and 28.8 mg/day) on blood viscosity and erythrocyte deformability in 27 patients suffering from cerebrovascular insufficiency. The patients were divided
INTRODUCTION: Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative nickel mesh filtration technique. METHODS: Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients (n = 101) and age-matched normotensive subjects (n = 14) were obtained from medical records, and diabetic patients were excluded. RESULTS: Erythrocyte deformability in the hypertensive group was significantly (p = 0.010) lower (87.8 +/- 2.2 %) than that ...
Dive into the research topics of High-dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass. Together they form a unique fingerprint. ...
This pilot study aimed to evaluate the differential effects of a remote ischemic preconditioning (rIPC) manoeuvre on performance and red blood cell (RBC) deformability compared to a sham control and a placebo setting. Ten male subjects performed three test settings in a single-blind, crossover, and randomized control design. All settings started with 20 min of rest and were followed by 4 cycles of occlusion/reperfusion consisting of 5 min each. During rIPC and placebo, the cuff pressure was inflated to 200 mmHg and 120 mmHg, respectively. During the sham control setting, 10 mmHg pressure was applied. All tests were followed by a cycle exercise with lactate diagnostics. Power at 2 and 4 mmol/l lactate thresholds were calculated. RBC deformability was measured before and after the respective manoeuvre. Results showed that no effect resulted from any manoeuvre on performance values or RBC deformability. But 6 subjects showed a higher power at the 2 mmol/l threshold, and 5 subjects exerted higher ...
Hemolytic anemia is one of the hallmarks of malaria and leads to an increase in oxidized heme (hemin) within the plasma of infected individuals. While scavenger proteins sequester much of the circulating heme, it has been hypothesized that extracellular heme may play a central role in malaria pathogenesis. W CSC100: Celebrating Canadian Chemistry
Reduced deformability of red blood cells (RBCs) may play an important role on the pathogenesis of chronic vascular complications of diabetes mellitus. However, available techniques for measuring RBC deformability often require washing process after each measurement, which is not optimal for day-to-day clinical use at point of care. The objectives of the present study are to develop a device and to delineate the correlation of impaired RBC deformability with diabetic nephropathy. We developed a disposable ektacytometry to measure RBC deformability, which adopted a laser diffraction technique and slit rheometry. The essential features of this design are its simplicity (ease of operation and no moving parts) and a disposable element which is in contact with the blood sample. We studied adult diabetic patients divided into three groups according to diabetic complications. Group I comprised 57 diabetic patients with normal renal function. Group II comprised 26 diabetic patients with chronic renal ...
Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certain inflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, our goal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore we performed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in order to corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with ex vivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology. Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA) were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light and analyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates. Conformational changes of
Dao and his colleagues are adapting sophisticated nanoscale measurement tools developed at the Nanomechanics Lab in conjunction with new biomechanical models to explore the behavior of host red blood cells infected by the deadliest of the malarial parasites, Plasmodium falciparum. The findings are helping to establish novel diagnostic devices as well as to identify new drugs and treatment targets that could eventually disrupt malaria-infection-induced changes in red blood cell deformability, stickiness, and shape. Altering these traits could potentially limit both the effects of malaria and the chances for infection ...
The potential for circulating tumor cells (CTCs) to elucidate the process of cancer metastasis and inform clinical decision-making has made their isolation of great importance. However, CTCs are rare in the blood, and universal properties with which to identify them remain elusive. As technological advancements have made single-cell deformability measurements increasingly routine, the assessment of physical distinctions between tumor cells and blood cells may provide insight into the feasibility of deformability-based methods for identifying CTCs in patient blood. To this end, we present an initial study assessing deformability differences between tumor cells and blood cells, indicated by the length of time required for them to pass through a microfluidic constriction. Here, we demonstrate that deformability changes in tumor cells that have undergone phenotypic shifts are small compared to differences between tumor cell lines and blood cells. Additionally, in a syngeneic mouse tumor model, cells ...
Cellular adhesion plays a critical role in biological systems and biomedical applications. Cell deformation and biophysical properties of adhesion molecules are of significance for the adhesion...
Abstract of Paper: Deformability of Erythrocytes and Factors Affecting it From Childhood to Old Age: A Comparative Study , Author: MOHAMED T. EL-GENGEHY, M.D.; ALAA E. IBRAHIM, M.D.; MOHAMED A. MOSTAFA, M.D.; NAZEH A. EL-NADY, M.D.; SALAH E. EL-ASHMAWY, M.D. and TAISSER K. EIADA, M.D. , Year: 1995 , Faculty of Medicine, Benha University
This thesis presents a systematic numerical modeling framework to simulate the stress-deformation and coupled stress-deformation-flow processes by performing uniaxial and biaxial compressive tests on fractured rock models with considering the effects of different loading conditions, different loading directions (anisotropy), and coupled hydro-mechanical processes for evaluating strength and deformability behavior of fractured rocks. By using code UDEC of discrete element method (DEM), a series of numerical experiments were conducted on discrete fracture network models (DFN) at an established representative elementary volume (REV), based on realistic geometrical and mechanical data of fracture systems from field mapping at Sellafield, UK. The results were used to estimate the equivalent Youngs modulus and Poissons ratio and to fit the Mohr-Coulomb and Hoek-Brown failure criteria, represented by equivalent material properties defining these two criteria.. The results demonstrate that strength ...
Ektacytometry was performed on RBC from the patient and his parents. Whole blood was mixed with isotonic buffer containing either Na+ or K+ ions and freed of white cells at room temperature. The suspensions were kept on ice, sequentially centrifuged and subjected to ektacytometry (1 h to 2 h) (Figure 1A). The osmoscans of both parents were super-imposable whether cells were pre-incubated with Na+- or K+-containing buffer. While the osmoscans of RBC from the mother (BN, BK) displayed a nearly normal pattern [see the corresponding control scan (con)], the two osmoscans from his fathers RBC (CN, CK) showed a left shift, implying a higher surface/volume ratio than normally observed, indicative of macrocytosis. The osmoscans from the patients RBC differed considerably from those of his parents and varied depending on the cation composition of the buffer (Figure 1A: AN, AK). When kept in Na+-containing buffer (AN), the RBC revealed a pattern similar to that seen for RBC from patients with hereditary ...
All models are wrong, but some are useful. This famous saying mirrors the situation in cell mechanics as well. It looks like no particular model of the cell deformability can be unconditionally preferred over others and different models reveal different aspects of the mechanical behavior of living cells. The purpose of the present work is to discuss the so-called tensegrity models of the cell cytoskeleton. It seems that the role of the cytoskeleton in the overall mechanical response of the cell was not appreciated until Donald Ingber put a strong emphasis on it. ...
Sartopore®2 XLIPore size: 0.35 , 0.2 µm (heterogeneous PES double layer). It is the identical final 0.2 µm membrane layer which ensures sterility of your product. But it is the pre-filter porosity which makes the difference. Depending on specific product properties a finer or coarser pre-filter layer can significantly improve the capacity of the filter. Quasi particle-free liquids like buffer solutions can be filtered without any pre-filter layer for increasing the flow performance.. If a new process is designed or existing processes are optimized, Sartopore® 2 allows cost reduction of up to 90%. The best-performer of your specific application can be easily identified by Increase® filterability trials. Make use of this service to understand the benefit for your filtration process. ...
Sartopore®2 XLIPore size: 0.35 , 0.2 µm (heterogeneous PES double layer). It is the identical final 0.2 µm membrane layer which ensures sterility of your product. But it is the pre-filter porosity which makes the difference. Depending on specific product properties a finer or coarser pre-filter layer can significantly improve the capacity of the filter. Quasi particle-free liquids like buffer solutions can be filtered without any pre-filter layer for increasing the flow performance.. If a new process is designed or existing processes are optimized, Sartopore® 2 allows cost reduction of up to 90%. The best-performer of your specific application can be easily identified by Increase® filterability trials. Make use of this service to understand the benefit for your filtration process. ...
Several aspects of the behaviour of polyoma virus-transformed BHK cells in culture have suggested that they are more deformable than BHK cells. This possibility was tested by applying negative pressure at the cell surface by means of a micropipette. It was found that PyBHK cells in early mitosis are twice as deformable as BHK cells in the same stage. In addition, the taut, non-ruffling margins of both cell types when fully spread are much less deformable than the extending, ruffling leading lamella. The degree of deformability of these cells is correlated with the distribution and organization of microfilaments and consistent with this, deformability increases greatly in the presence of cytochalasin B. The significance of deformability studies such as these is discussed. ...
TY - JOUR. T1 - Influence of rheologic changes and platelet-neutrophil interactions on cell filtration in sepsis. AU - Kirschenbaum, Linda A.. AU - Aziz, Mohammed. AU - Astiz, Mark E.. AU - Saha, Dhanonjoy C.. AU - Rackow, Eric C.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - We examined the role of erythrocyte (red blood cell; RBC) aggregation and deformability, neutrophil (polymorphonuclear neutrophil; PMN) deformability, whole-blood viscosity, and platelet-neutrophil interactions on cell filtration in subjects who were critically ill with sepsis (CIS), critically ill noninfected subjects (CINS), and healthy controls (C). We assessed cell deformability by filtration through filters of 5-μm pore size. Whole blood, RBC, PMN, and combinations of PMN and RBC were studied. Viscometry was done on isolated RBC. Platelet-PMN interactions were assessed with monoclonal antibodies to CD41 and activated CD53 platelet receptors, and to CD66b PMN receptors. Filtration pressure (Pi) for CIS was significantly greater ...
PubMed journal article Blood viscosity and erythrocyte filterability: their evaluation in diabetes mellitu were found in PRIME PubMed. Download Prime PubMed App to iPhone or iPad.
TY - JOUR. T1 - Effects of sulfhydryl inhibition on red blood cells. II. Studies in vivo.. AU - JACOB, H. S.. AU - JANDL, J. H.. PY - 1962/7. Y1 - 1962/7. UR - http://www.scopus.com/inward/record.url?scp=0002880017&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0002880017&partnerID=8YFLogxK. U2 - 10.1172/JCI104607. DO - 10.1172/JCI104607. M3 - Article. C2 - 14450645. AN - SCOPUS:0002880017. VL - 41. SP - 1514. EP - 1523. JO - Journal of Clinical Investigation. JF - Journal of Clinical Investigation. SN - 0021-9738. ER - ...
In the development of malaria, the parasite, Plasmodium falciparum invades the human red blood cell and affects the deformability and adhesion capabilities of the cell. Mortality in these cases is generally caused by blockage of small blood vessels. The research in the Suresh lab involves collaborations with engineers, biologists and medical colleagues, and it revolves around measurement of the deformability and its connection to development of the parasite inside the cell. They use continuous force- displacement variations as measured by optical tweezers or other direct mechanical assays to study different stages of parasite development. Optical tweezers or optical trap technology uses a laser beam, focused by a highquality microscope objective, to trap silica particles attached to a living cell. The deformability is measured by calculating the force required to move the particle with the laser beam and determine a force versus deformability diagram as a function of cellular changes introduced ...
A reduction of uniaxial compressive strength (UCS) by the presence of water has been ascertained in many sedimentary rocks and even some metamorphic rocks experimentally. This paper reports and discusses the influence of water content and anisotropy on the strength and deformability of two meta-sedimentary rocks by triaxial compressive tests. Before conducting the triaxial tests, the physical properties of meta-siltstone and meta-sandstone specimens have been carefully examined. The water contents of both tested rocks are very low, for instance, 0.17% for meta-siltstone and 0.10% for meta-sandstone. The porosities of the tested rocks are analyzed by the technique of mercury intrusion porosimetry (MIP). It shows that the meta-sedimentary rocks have very low porosity, 0.18% for meta-siltstone and 0.53% for meta-sandstone on average. The thin section analysis reveals that the meta-siltstone contains more hydrophilic substances such as clay minerals than the meta-sandstone, and both two ...
Tzitzinou, A, Keddie, JL, Geurts, JM, Peters, ACIA and Satguru, R (2000) Film formation of latex blends with bimodal particle size distributions: Consideration of particle deformability and continuity of the dispersed phase ...
Single-cell deformability has been recently identified as a critical biomarker for various diseases and it varies considerably based on phenotypes. Current cell deformability measurement techniques, however, are inherently low throughput for statistical analysis of large heterogeneous biological samples. We focus on developing high-throughput microfluidic techniques for measuring intrinsic properties of single cells, including intracellular viscosity, membrane tension/elasticity and Youngs modulus. These measurements will allow us to identify potential genetic-alterations and phenotype-changes, responsible for modification in such properties of cells. Furthermore, systematic determination of single-cell mechanical properties in a rapid and standardized manner will expedite an adoption of aforementioned properties as new types of biomarkers for phenotype characterizations. These newly revealed biomarkers should provide efficient tools for determining the cell state and phenotype, which are ...
Human red blood cells, scanning electron micrograph (SEM). Red blood cells (RBCs), or erythrocytes, are the most common type of blood cell in vertebrates. RBCs take up oxygen in the lungs or gills and release it into tissues while squeezing through the bodys capillaries. The cytoplasm of RBCs is rich in haemoglobin, an iron-containing biomolecule that can bind oxygen and is responsible for the red colour of the cells. The cell membrane is composed of proteins and lipids and this structure provides properties essential for physiological cell function (deformability and stability) while traversing the circulatory system, especially the capillary network. In humans, mature RBCs are flexible and oval biconcave disks. RBCs are the most abundant type of cell in human blood, accounting for 40% of the blood volume. Magnification: x1,600 when shortest axis printed at 25 millimetres. - Stock Image C037/0700
Gas Separation Membranes. With the rise of energy costs, membrane technology for separating gases plays a significant role in reducing the environmental impact and costs of industrial processes. Polymer-based gas separation membranes offer advantages over conventional technologies (cryogenic distillation, amine absorption, etc.) including, low capital and operating costs, minimum energy requirements, ease of operation, and environmental friendliness. However, gas separation in polymers have reached a seemingly reached the trade-off between permeability and selectivity, as plotted on the 2008 Robeson plot. Inorganic membranes, on the other hand, display excellent permselectivity properties but incur high fabrication costs and lack membrane mechanical stability. Mixed-matrix membranes (MMMs) comprising thermally stable polymers and inorganic additives for target gases combines the polymers mechanical properties with the additives permselectivity properties. Our group focuses on MMMs consisting ...
At first glance, quite a few tissues in simple animals or in the early development look similar to an assembly of soap bubbles. The undeniable appeal of this correspondence, the seemingly elementary surface-tension Hamiltonian, and the prospect of a mechanical model of biological structures have fueled resea
SOLUTION: A physicist by training, Guck used laser beams in his graduate work to study the physical properties of cells. Not all cells are equally squishy, he found: while normal cells are relatively rigid, cancer cells are more pliable. The more aggressive the cell, the softer it is, and that may be necessary for it to pass into tissues, Guck explains.. He suspected it might be possible to sort otherwise identical cells based on this phenomenon-and to do so without antibodies. Some interesting cell types and cancers have no known unique molecular identifiers, Guck notes. But deformability is an inherent property of cells, enabling label-free cell analysis. The cell cannot help but have that property. Researchers had already developed one approach called deformability cytometry (DC), in which flowing cells smash head on into a wall of flowing liquid, deforming like crash-test dummies. Using an ultrahigh-speed camera, the method monitors 1,000 cells/second, but only for a few seconds, as ...
4.. M. A. Krishtal and A. P. Mokrov, Hardening of layers formed during diffusion of molybdenum in iron and its alloys, Fiz. Metal. Metal.12, 3, 385 (1961).. ...
I used Baking Soda / Black strap Molasses Protocol. Mixed 1 Tea SP BS with 1 tea SP Molasses in 125ml water at 110 F or 43C for 5 minutes, added water to 300ml and drink 7AM and 7 PM you can t go mote than a week like that. After I used 1 BC 1 Molasses per day for one more week. After a 4 week break, using one lemon in a glass of water, I started BS again. Now my saliva/urine pH is 7.5. I have a BS in chemistry and know how to play the dangerous game with baking soda. You may consult your doctor ...
Using the DPD code built on an open-source software called LAMMPS (Large-scale Atomic/Molecular Massively Parallel Simulator) by Sandia National Laboratories, Peng says he and his co-researchers discovered that the surface area of RBCs plays an essential role in whether an RBC will pass the inter-endothelial slits. They found that a small reduction in RBC surface area (5 percent, for example) significantly increases the critical pressure necessary for RBCs to pass the spleen.. The form and behavior of an RBC, Peng explains, could, in certain respects, be considered analogous to a basketball. If air is added to a basketball, it becomes inflated and rigid; if the volume of air is decreased, the ball becomes much softer. Although an RBC is inflated with water rather than air, the principle is the same, he adds. Another important consideration in the dynamics, he notes, is the material of which the ball or the RBC consists, especially in terms of the membrane rigidity of the ball or the RBC.. Peng ...
Deformability of both individual base pairs and dimer steps is important for DNA sequence recognition. Parameters describing base-pair and dimer step geometry may be correlated with each other due to stereochemical interactions of adjacent bases in dimer
Six-row barley is the most economical to grow because the greater number of rows per head increases the per-acre yield. It is a warm-climate barley and is the type most widely grown in the United States. It is favored by domestic commercial breweries because it is rich in the enzymes needed to convert the adjuncts they employ (adjuncts may account for up to 60 percent of the extract in U.S. brews). Because six-row barley is high in protein, in all-malt brews it presents problems with clarity and stability. Its higher husk content improves mash filterability but can contribute harsh flavors, especially when brew-house practices cause hot-side aeration ...
Hemorheology, also spelled haemorheology (from the Greek αἷμα, haima blood and rheology), or blood rheology, is the study of flow properties of blood and its elements of plasma and cells. Proper tissue perfusion can occur only when bloods rheological properties are within certain levels. Alterations of these properties play significant roles in disease processes. Blood viscosity is determined by plasma viscosity, hematocrit (volume fraction of red blood cell, which constitute 99.9% of the cellular elements) and mechanical properties of red blood cells. Red blood cells have unique mechanical behavior, which can be discussed under the terms erythrocyte deformability and erythrocyte aggregation. Because of that, blood behaves as a non-Newtonian fluid. As such, the viscosity of blood varies with shear rate. Blood becomes less viscous at high shear rates like those experienced with increased flow such as during exercise or in peak-systole. Therefore, blood is a shear-thinning fluid. ...
Rheology - the study of the flow of matter and accompanying phenomena of real bodies deformation - in relation to blood - hemorheology. Blood viscosity - the main rheological parameter - has been studied in many research centers and among many different group of patients. The main disorders related to the hemorheological properties are: coronary insufficiency, vascular congestion, myocardial infarction, cerebral circulation disorder, Reynaud disease, ischemic limbs, diabetes, anemia, tumors. The following parameters are the main blood viscosity determinants: plasma viscosity, hematocrit, red cell deformability and erythrocytes aggregation. In hemorheological studies we used mathematical rheological models. The measurements of blood and plasma viscosity are performed by means of oscillating-rotary rheometers in order to determine the dependence of blood viscosity on the shear rate and the two components of the complex blood viscosity. Determination of blood cells aggregability and deformability ...
DESIGN: Randomized controlled trial.. METHODS: This research was carried out between April 2013 and January 2014 in the second hospital of Xiangya, Central South University in Changsha, China. Initially 96 cancer participants with PICC were chosen and randomly allocated to experimental and control group. The participants of the experimental group were conducted route PICC maintain technique and took acetylsalicylic acid 100 mg per day after dinner, while the control group received route PICC maintain technique only. The infusion speed and hemorheology indexes of the two groups were tested before our study and at the end of the 2nd and 4th months with several instruments.. RESULTS: Repeated measures analysis of variance indicated that taking acetylsalicylic acid orally had significant main effect on high shear blood viscosity and red blood cell deformability index (P , 0.05), and it also had significant main effect as well as time effect on plasma viscosity (P , 0.05); and time had significant ...
There are several applications for non-thermal plasma at atmospheric pressure, such as the sterilization of infected tissues, wound healing, and delicate surgery. Non-thermal plasma generates reactive oxygen/nitrogen species (RONS). In this study, we examined human red blood cells (RBCs), platelet function, and blood coagulation, following treatments with different feeding gases (air, nitrogen (N2), and argon (Ar)) of the dielectric barrier discharge plasma (DBDP) at different exposure times. This is the first study to evaluate the effects of DBDP on hemolysis, deformability, aggregation, and morphology of RBCs, as well as platelet function and blood coagulation at three doses (5, 10 and 15 min exposure). In comparison to N2 and Ar DBDP, air DBDP at more than 10 min exposure to RBC in culture plate with 2 ml liquid solution caused hemolysis and alterations in RBC deformability and aggregation owing to the higher levels of hydrogen peroxide, hydroxyl oxidants and other RONS. Moreover, air DBDP ...
Recent studies have revealed that functional hyperemia is initiated in capillaries (14, 15) and that RBCs themselves can act as oxygen-sensing regulators to control capillary RBC velocity in response to local PO2 changes (15). However, the underlying mechanisms of how PO2 could modulate RBC velocity in capillaries are unknown. Experimental findings presented here implicate that deoxyHb-band 3 interactions in RBCs are the molecular switch that responds to local PO2 changes and controls RBC deformability and consequently RBC capillary velocity. Evidently, we showed that (i) while ex vivo capillary velocity and deformation of mRBC-WT and mRBC-subst 1-35 exhibited a linear relation with local PO2 changes, RBCs from transgenic mice that had enhanced or weakened deoxyHb-band 3 interactions showed capillary velocity and deformation independent of PO2. RBCs with enhanced deoxyHb-band 3 interactions showed higher capillary velocity and deformability compared to RBCs that had weakened deoxyHb-band 3 ...
Abstract. Rheologic properties of blood were studied in 8 patients with dilated cardiomyopathy (DCM) and in 10 healthy subjects. Whole-blood viscosity was measured at four different shear rates, by means of a computer-controlled rotational viscometer. The patients had significantly higher blood viscosity at all shear rates, both at their natural hematocrits and after an in vitro adjustment of sample hematocrits to 45%. Erythrocyte filterability (5 μm pore size) was significantly lower, fibrinogen concentration significantly higher, and HDLcholesterol concentration significantly lower in the patient group. No significant differences were found regarding hematocrit, mean corpuscular volume, haemoglobin concentration, leukocyte count and filterability (8 μm pore size), plasma viscosity, and total cholesterol concentration.. The measured hemorheologic abnormalities may contribute to the previously reported reduction of coronary blood flow reserve in DCM patients and to myocardial microcirculatory ...
An elevated concentration of fibrinogen in blood is a significant risk factor during many pathological diseases, as it leads to an increase in red blood cells (RBC) aggregation, resulting in hemorheological disorders. Despite the biomedical importance, the mechanisms of fibrinogen-induced RBC aggregation are still debatable. One of the discussed models is the non-specific adsorption of fibrinogen macromolecules onto the RBC membrane, leading to the cells bridging in aggregates. However, recent works point to the specific character of the interaction between fibrinogen and the RBC membrane. Fibrinogen is the major physiological ligand of glycoproteins receptors IIbIIIa (GPIIbIIIa or αIIββ3 or CD41/CD61). Inhibitors of GPIIbIIIa are widely used in clinics for the treatment of various cardiovascular diseases as antiplatelets agents preventing the platelets’ aggregation. However, the effects of GPIIbIIIa inhibition on RBC aggregation are not sufficiently well studied. The objective of
Sickle cell disease (SCD) is a genetic disorder caused by inheritance of two alleles bearing a single nucleotide change in the β globin gene coding sequence. The pathophysiologic mechanism of SCD involves polymerization of intracellular hemoglobin S (HbS) following deoxygenation in the microvasculature, leading to decreased red blood cell (RBC) deformability, morphologic sickling of RBCs, decreased RBC survival, microvascular obstruction, and clinical complications (Bunn H, N Engl J Med, 1997). Voxelotor, a hemoglobin S polymerization inhibitor recently approved for the treatment of SCD, is an allosteric modifier of Hb that increases the proportion of oxygenated Hb in all RBCs. In clinical studies in subjects with SCD, voxelotor has demonstrated that doses of up to 1500 mg daily achieved Hb modifications of ~27%, was well tolerated, and resulted in reduced hemolytic anemia (Howard J et al., Blood, 2019; Vichinsky E et al., N Engl J Med, 2019).. GBT021601 is a potent second generation HbS ...
Summary The foregoing experiment with cultures of Leptospira icteroides furnishes conclusive confirmatory evidence of the filterability of the organism. Whether it passes through the filter in its spiral form, assisted by its active motility, or in a granular phase, further experimental evidence may determine.
How does vitamin B12 affect red blood cells? Having vitamin B12 deficiency means that your body does not have enough of this vitamin. You need B12 to make
The normal range for a white blood cell count can vary from person to person. Low blood cell counts can lead to serious complications that may delay your next
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Red blood cells in a capillary (human central nervous system), coloured transmission electron micrograph (TEM). Red blood cells (RBCs), or erythrocytes, are the most common type of blood cell in vertebrates. They are involved in delivering oxygen to the body tissue. RBCs take up oxygen in the lungs or gills and release it into tissues while squeezing through the bodys capillaries. The cytoplasm of RBCs is rich in haemoglobin, an iron-containing biomolecule that can bind oxygen and is responsible for the red color of the cells. The cell membrane is composed of proteins and lipids and this structure provides properties essential for physiological cell function (deformability and stability) while traversing the circulatory system, especially the capillary network. In humans, mature RBCs are flexible and oval biconcave disks. Capillaries are the small blood vessels that make up the microcirculation of the human body. Magnification: x2,130 when shortest - Stock Image C032/0833
Band 3 mutations associated with HSt often produce an increased cation leak at low temperature (cryohydrocytosis; CHC). Patients with these variants show mild to moderate hemolytic anemia. Again, it is thought that the mutations responsible (Ser731Pro and His734Gln) affect the normal folding of band 3 and convert the protein into a cation conductor.4 Another mutation in band 3 has been identified as responsible for HSt Blackburn (Leu687Pro), in which affected erythrocytes share features of CHC and familial pseudohyperkalemia (see below), but the cation leak is not increased at low temperatures.4 Other band 3 mutants, originally classified as HS, have been reclassified as hereditary spherocytosis with a low temperature leak (HS-LTL) (Asp705Tyr and Arg760Gln) due to their increased cation leak at low temperatures.4 The known HSt point mutations in band 3 are illustrated in Figure 2, and occur in a restricted region of the trans-membrane domain of the protein. In the current issue of the journal, ...
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This review focuses on the analysis and evaluation of the diverse senescence markers suggested to prime red blood cells (RBC) for clearance in humans. These tags develop in the course of biochemical and structural alterations accompanying RBC aging, as the decrease of activities of multiple enzymes, the gradual accumulation of oxidative damage, the loss of membrane in form of microvesicles, the redistribution of ions and alterations in cell volume, density, and deformability. The actual tags represent the penultimate galactosyl residues, revealed by desialylation of glycophorins, or the aggregates of the anion exchanger (band 3 protein) to which anti-galactose antibodies bind in the first and anti-band 3 naturally occurring antibodies (NAbs) in the second case. While anti-band 3 NAbs bind to the carbohydrate-free portion of band 3 aggregates in healthy humans, induced anti-lactoferrin antibodies bind to the carbohydrate-containing portion of band 3 and along with anti-band 3 NAbs may accelerated ...
PFM on Red Blood Cell. Left: Topographic image of red blood cells, 30µm scan.. Right: Close-up of individual cell outlined in larger scan (left) and labeled as II. The piezoresponse ...
The application of steel fiber reinforced polymer (SRP) as a means of increasing the capacity of masonry columns is investigated in this study. The behavior of 23 solid-brick specimens that are externally wrapped by SRP sheets in low volumetric ratios is presented. The specimens are subjected to axial monotonic load until failure occurs. Two widely used types of masonry columns of differing square cross-sections were tested in compression (square and octagonal cross-sections). It is concluded that SRP-confined masonry behaves very much like fiber reinforced polymers (FRP)-confined masonry. Confinement increases both the load-carrying capacity and the deformability of masonry almost linearly with average confining stress. A comparative analysis between experimental and theoretical values computed in compliance with the Italian Council of Research (CNR) was also developed.
Capillary Suction Time Apparatus - Model 304B CST (). The Capillary Suction Time has been used since the 1970s as a quick and reliable method for characterising sludge filterability and conditionability.
Capillary Suction Time Apparatus - Model 304M CST (). The capillary Suction Time has been used since the 1970s as a quick and reliable method for characterising sludge filterability and conditionability.
Rheology concerns itself with materials that have aspects of both solids and liquids, and researches the flow and deformation of matter. Malvern Instr
The main function of RBCs is the transport of oxygen from the lungs to body tissues. All of the cells in this Wright-stained peripheral blood smear are normal RBCs.. ...
Anemia is a condition that occurs when your body does not have enough healthy red blood cells (RBCs). Your RBCs are the parts of your blood that carry oxygen all over your body. Without enough RBCs, your body doesnt get enough oxygen.
The Presidential Steering Committee (PSC) on COVID-19 has put six states and the FCT on red alert to ensure a safe and healthy nationwide Eid-al-Adha celebration. This…
Correlates local cortical fluorescence with membrane movement. Quimp is based on the electrostatic contour migration method (ECMM) that consists of an improvement of a boundary tracking approach. It reduces sum of path lengths connecting all pairs of points, equivalent to minimizing the energy required for cell deformation. This tool is useful to study time series of several hundreds of cells per experimental condition.
erythrocyte deformability under shear. Due to a special phenomenon called tank treading, the membrane of the erythrocyte (red ... making it possible to measure the erythrocyte deformability and the orientability of the cells. In an ektacytometer erythrocyte ... deformability can be measured under changing osmotic stress or oxygen tension and is used in the diagnosis and follow up of ...
"Decreased erythrocyte deformability after transfusion and the effects of erythrocyte storage duration". Anesthesia and ... is exploration of potentially relevant tests based on RBC membrane properties such as erythrocyte deformability and erythrocyte ...
"Decreased erythrocyte deformability after transfusion and the effects of erythrocyte storage duration". Anesthesia and ... though there is exploration of potentially relevant tests based on RBC membrane properties such as erythrocyte deformability[46 ... Compatibility of ABO and Rh system for Red Cell (Erythrocyte) Transfusion[edit]. This chart shows possible matches in blood ... 2.3.1 Compatibility of ABO and Rh system for Red Cell (Erythrocyte) Transfusion ...
... may facilitate the deformability of erythrocytes in capillary which is useful for cardiovascular disease. Peripheral ... Piracetam has been found to diminish erythrocyte adhesion to vascular wall endothelium, making any vasospasm in the capillary ...
... which can be discussed under the terms erythrocyte deformability and erythrocyte aggregation. Because of that, blood behaves as ... The presence of cells with diminished deformability, as is the case in sickle cell disease, tends to inhibit the formation of ... With very low shear rates, the viscoelastic property of blood is dominated by the aggregation and cell deformability is ... The viscoelasticity of the blood is dominated by the deformability of the red blood cells. Maxwell Model concerns Maxwell ...
erythrocyte+membrane+band+4.1+protein at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing ... "Entrez Gene: EPB41 erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)". Hung LY, Tang CJ, Tang TK (October ... Implications for erythrocyte membrane stability". J. Clin. Invest. 68 (2): 454-60. doi:10.1172/JCI110275. PMC 370818. PMID ...
The sickled erythrocytes present a decreased deformability when compared to normal erythrocytes, leading to distress in ... deformability can be used to evaluate the amount of sickled cells in the blood. Deformability of the erythrocytes that cause ... In order to determine the deformability of erythrocytes multiple factors including blood and plasma viscosity and hematocrit (a ... Normal hemoglobin (and hemoglobin S in the presence of oxygen) contains a deformability characteristic that allows erythrocytes ...
"Decreased erythrocyte deformability after transfusion and the effects of erythrocyte storage duration". Anesthesia and ... though there is exploration of potentially relevant tests based on RBC membrane properties such as erythrocyte deformability[29 ... and erythrocyte fragility (mechanical).[30]. Many physicians have adopted a so-called "restrictive protocol"-whereby ...
... erythrocyte volume MeSH G09.188.250.313.610 - plasma volume MeSH G09.188.250.340 - erythrocyte deformability MeSH G09.188. ... erythrocyte aggregation MeSH G09.188.261.454 - erythrocyte aging MeSH G09.188.261.544 - hematopoiesis MeSH G09.188.261.544.414 ... erythrocyte volume MeSH G09.330.553.400.214.610 - plasma volume MeSH G09.330.553.400.280 - cardiac output MeSH G09.330.553.400. ... erythrocyte count MeSH G09.188.250.161.330.725 - reticulocyte count MeSH G09.188.250.161.595 - leukocyte count MeSH G09.188. ...
... including erythrocyte deformability and cell morphology. Morphology can be measured by indexes which characterize shape changes ... Erythrocyte fragility refers to the propensity of erythrocytes (red blood cells, RBC) to hemolyse (rupture) under stress. It ... Deformability testing involves measuring the degree or ease of cells' contortion or shape change under a given level of applied ... Uses of erythrocyte mechanical fragility can include diagnostic testing, calibrations to aid comparisons of hemolysis caused by ...
... refers to the ability of erythrocytes (red blood cells, RBC) to change shape under a given level of ... Decreased Erythrocyte Deformability After Transfusion and the Effects of Erythrocyte Storage Duration, Anesth Analg, published ... No other cells of mammalian organisms have deformability comparable with erythrocytes; furthermore, non-mammalian erythrocytes ... and the orientation of erythrocytes with the applied forces. Deformability is an intrinsic cellular property of erythrocytes ...
"Erythrocyte Deformability As a Potential Biomarker for Chronic Fatigue Syndrome". Blood. 132 (Suppl 1): 4874. doi:10.1182/blood ... Erythrocyte sedimentation rate (ESR) is the measure of ability of erythrocytes (red blood cell) to fall through the blood ... The erythrocyte sedimentation rate (ESR or sed rate) is the rate at which red blood cells in anticoagulated whole blood descend ... The rate of erythrocyte sedimentation is affected by both inflammatory and non-inflammatory conditions. In inflammatory ...
The cytoplasm of erythrocytes is rich in hemoglobin, an iron-containing biomolecule that can bind oxygen and is responsible for ... The proteins of the membrane skeleton are responsible for the deformability, flexibility and durability of the red blood cell, ... In plasma about 5% of CO2 is in physical solution 94% as bicarbonate and 1% as carbamino compounds; in the erythrocytes the ... Approximately 2.4 million new erythrocytes are produced per second in human adults. The cells develop in the bone marrow and ...
Erythrocyte deformability. *Erythrocyte fragility. *Blood substitute. *Packed red blood cells. *Red blood cell indices ... Red blood cells, also known as RBCs, red cells,[1] red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek ... Harrison, K. L. (1979). "Fetal Erythrocyte Lifespan". Journal of Paediatrics and Child Health. 15 (2): 96-97. doi:10.1111/j. ... Föller M, Huber SM, Lang F (October 2008). "Erythrocyte programmed cell death". IUBMB Life. 60 (10): 661-8. doi:10.1002/iub.106 ...
Spectrin alpha chain, erythrocyte is a protein that in humans is encoded by the SPTA1 gene. Spectrin is an actin crosslinking ... which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a ... 1991). "The exon-intron organization of the human erythrocyte alpha-spectrin gene". Genomics. 9 (1): 131-40. doi:10.1016/0888- ...
... is the presence in the blood of spherocytes, i.e erythrocytes (red blood cells) that are sphere-shaped rather ... spherocytosis has an attribute of decreased cell deformability. Spherocytosis can be diagnosed in Peripheral blood film by ...
The Parsortix method separates CTCs based on their size and deformability. Hybrid methods combine physical separation (by ... blood cells are prepared by only one centrifugation step and erythrocyte lysis. Like CellSearch, maintrac uses an EpCAM ...
This shortage of erythrocytes results in hemolytic anemia. It was first described in 1871. It is the most common cause of ... Both pathways result in membrane loss, decreased surface area, and formation of spherocytes with decreased deformability. As ... The disorder is caused by mutations in genes relating to membrane proteins that allow for the erythrocytes to change shape. The ... Hereditary spherocytosis is an abnormality of red blood cells, or erythrocytes. A chronic disease with a long term health ...
There are also effects associated with deformability of red blood cells that might increase this force. It is clear that red ... This is because erythrocytes move over to the centre of the vessel, leaving only plasma near the wall of the vessel. The effect ...
A neutrophil with a segmented nucleus (center and surrounded by erythrocytes), the intra-cellular granules are visible in the ... Linderkamp, Otwin; Ruef, Peter; Brenner, Birgit; Gulbins, Erich; Lang, Florian (1998). "Passive Deformability of Mature, ...
i.e. human erythrocytes and mouse fibroblasts. A recent test has seen great success in differentiating cancerous cells from non ... "Optical Deformability as an Inherent Cell Marker for Testing Malignant Transformation and Metastatic Competence". Biophys. J. ...
Erythrocyte deformability. *Erythrocyte fragility. *Blood substitute. *Packed red blood cells. *Red blood cell indices ... Red blood cells, also known as RBCs, red cells,[1] red blood corpuscles, haematids, erythroid cells or erythrocytes (from Greek ... Harrison, K. L. (1979). "Fetal Erythrocyte Lifespan". Journal of Paediatrics and Child Health. 15 (2): 96-97. doi:10.1111/j. ... Föller M, Huber SM, Lang F (October 2008). "Erythrocyte programmed cell death". IUBMB Life. 60 (10): 661-8. doi:10.1002/iub.106 ...
Erythrocyte deformability refers to the ability of erythrocytes (red blood cells, RBC) to change shape under a given level of ... Decreased Erythrocyte Deformability After Transfusion and the Effects of Erythrocyte Storage Duration, Anesth Analg, published ... No other cells of mammalian organisms have deformability comparable with erythrocytes; furthermore, non-mammalian erythrocytes ... and the orientation of erythrocytes with the applied forces. Deformability is an intrinsic cellular property of erythrocytes ...
Erythrocyte deformability was measured using a laser diffractoscope in the shear stress range 0.3-60 Pa. Results. Erythrocyte ... C-Peptide and Its C-Terminal Fragments Improve Erythrocyte Deformability in Type 1 Diabetes Patients. Thomas Hach,1,2 Thomas ... C-peptide and its C-terminal fragments are equally effective in improving erythrocyte deformability in type 1 diabetes. The C- ... This study aimed to investigate the influence of C-peptide and fragments thereof on erythrocyte deformability and to elucidate ...
In this chapter, we will demonstrate how microfluidic devices can be used to examine the vascular deformability of erythrocytes ... The significance of cellular deformability is readily apparent based solely on the disparate mean size of human erythrocytes (~ ... Changes in the deformability of either cell will result in their premature mechanical clearance as well as an enhanced ... Assessing the Vascular Deformability of Erythrocytes and Leukocytes: From Micropipettes to Microfluidics. By Mark D. Scott, ...
Our investigation regarded the behavior of whole-blood viscosity, plasma viscosity and erythrocyte deformability in MM. We ... Erythrocyte deformability and hemorheological profile in multiple myeloma Article type: Research Article ... Our investigation regarded the behavior of whole-blood viscosity, plasma viscosity and erythrocyte deformability in MM. We ... A significant decrease in erythrocyte deformability, especially at low shear stresses, was found. We discuss some hypotheses ...
Deformability based cell. margination-A simple microfluidic design for malaria-infected erythrocyte. separation H. W. Hou, A. A ... Deformability based cell margination-A simple microfluidic design for malaria-infected erythrocyte separation† ... margination is mimicked for the separation of iRBCs from whole blood based on their reduced deformability. The malaria infected ...
... deformability is greatly affected by the osmolality, and maximum deformability, which is determined as maximal elongation index ... Osmotic deformability of erythrocytes at various shear stresses Article type: Research Article ... Abstract: Red blood cell (RBC) deformability is greatly affected by the osmolality, and maximum deformability, which is ... we examined osmotic RBC deformability over a range of shear stresses (0.5-20 Pa). We found that the RBC deformability at low ...
These findings imply that deformability is indeed an important pathophysiological quantity. Erythrocyte deformability for this ... THE SHEAR STRESSED NORMAL ERYTHROCYTE AS A MODEL DEFECT FOR DECREASED RED CELL DEFORMABILITY. ... Exposure of normal erythrocytes to super-physiologic but subhemolytic shear stresses results in decreased red cell ... The zero time pressure drop from the linear portion of this curve is the deformability indicator P(,0). Pressure vs. filtration ...
They have been shown to impact on the deformability of erythrocytes infected with sexual and asexual P. falciparum parasites, ... cAMP-Signalling Regulates Gametocyte-Infected Erythrocyte Deformability Required for Malaria Parasite Transmission Download PDF ... falciparum erythrocyte invasion by merozoites [23,24], or modulation of infected erythrocyte membrane permeability [25]. So far ... 2012) A switch in infected erythrocyte deformability at the maturation and blood circulation of Plasmodium falciparum ...
Erythrocyte Deformability Erythrocytes Filtration Humans Hypertension Medical Records Microcirculation Nickel Smoke Smoking ... and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability ... Deformability Erythrocytes Filtration Hypertension MeSH Terms expand_less. expand_more. Blood Pressure Coronary Artery Disease ... RESULTS: Erythrocyte deformability in the hypertensive group was significantly (p = 0.010) lower (87.8 +/- 2.2 %) than that of ...
Decreased deformability of erythrocytes in haemolytic anaemia associated with glucosephosphate isomerase deficiency.: ... The decrease of deformability at a pH of 6.8 was most pronounced in the splenectomized patients, and likewise in erythrocytes ... Decreased deformability of erythrocytes in haemolytic anaemia associated with glucosephosphate isomerase deficiency.. Authors * ... Deformability of erythrocytes from four patients with different types of glucosephosphate isomerase (D-glucose-6-phosphate ...
Analysis of published and authors data on the physiological role of deformability of erythrocytes, general ... Erythrocyte deformability: physiological aspects].. Authors * Zinchuk, V V Type. Published Article. Journal. Uspekhi ... Degradation of deformability of erythrocytes under various types of oxygen deficit deteriorates functioning of the system of ... Deformability of erythrocytes is a factor generating adequate the oxygen supply to tissue, and its degradation aggravates ...
The deformability of normal and hardened erythrocytes (obtained by heating blood at 49°C or by incubating erythrocytes in a ... The deformability of normal and hardened erythrocytes (obtained by heating blood at 49°C or by incubating erythrocytes in a ... The deformability of normal and hardened erythrocytes (obtained by heating blood at 49°C or by incubating erythrocytes in a ... The deformability of normal and hardened erythrocytes (obtained by heating blood at 49°C or by incubating erythrocytes in a ...
Buchwald, H., & Menchaca, H. J. (2013). Oxygen transport rate and erythrocyte deformability. Anesthesia and analgesia, 117(5). ... Oxygen transport rate and erythrocyte deformability. / Buchwald, Henry; Menchaca, Hector J.. In: Anesthesia and analgesia, Vol ... Buchwald, H & Menchaca, HJ 2013, Oxygen transport rate and erythrocyte deformability, Anesthesia and analgesia, vol. 117, no ... Buchwald, Henry ; Menchaca, Hector J. / Oxygen transport rate and erythrocyte deformability. In: Anesthesia and analgesia. 2013 ...
... erythrocytes is direct signature of their membrane deformability. When placed in hypertonic buffer, discocytic erythrocytes are ... Khan, Manas and Soni, Harsh and Sood, AK (2009) Optical tweezer for probing erythrocyte membrane deformability. In: Applied ... A change in the deformability of the erythrocytes, due to any internal or environmental factor, affects the rotation speed of ... Here we show how the increment in erythrocyte membrane rigidity with adsorption of Ca++ ions can be exhibited through this ...
On-chip erythrocyte deformability test under optical pressure. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. ... On-chip erythrocyte deformability test under optical pressure. Won Gu Lee, Hyunwoo Bang, Hoyoung Yun, Joonmo Lee, Junha Park, ... On-chip erythrocyte deformability test under optical pressure. Lab on a Chip - Miniaturisation for Chemistry and Biology. 2007 ... On-chip erythrocyte deformability test under optical pressure. / Lee, Won Gu; Bang, Hyunwoo; Yun, Hoyoung; Lee, Joonmo; Park, ...
Ischemic Preconditioning Enhances Performance and Erythrocyte Deformability of Responders. Publikationen: Beitrag in ... RBC deformability was measured before and after the respective manoeuvre. Results showed that no effect resulted from any ... In these responsive subjects, RBC deformability also improved. Hence, rIPC effects are much influenced by the subjects ... deformability compared to a sham control and a placebo setting. Ten male subjects performed three test settings in a single- ...
A switch in infected erythrocyte deformability at the maturation and blood circulation of Plasmodium falciparum transmission ... We hypothesize that mechanical retention contributes to sequestration of immature GIEs and that regained deformability of ... and that a switch in cellular deformability in the transition from immature to mature gametocytes is accompanied by the ... Plasmodium falciparum gametocytes in the human host takes several days during which immature gametocyte-infected erythrocytes ( ...
Effects of erythrocyte deformability and aggregation on the cell free layer and apparent viscosity of microscopic blood flows. ... Effects of erythrocyte deformability and aggregation on the cell free layer and apparent viscosity of microscopic blood flows. ... Zhang, J., Johnson, P. C., & Popel, A. S. (2009). Effects of erythrocyte deformability and aggregation on the cell free layer ... Effects of erythrocyte deformability and aggregation on the cell free layer and apparent viscosity of microscopic blood flows. ...
... deformability after the removal of tourniquet compared to the control sample was observed. RBC deformability was maximal in the ... In conclusion, this study revealed that RBC deformability and aggregation might be significantly altered in the samples ... red blood cell deformability and aggregation indices) assessed in blood samples obtained after 5, 30, 60 and 90 s following the ...
... to human erythrocytes results in a marked decrease in cellular deformability as measured with a coun ... C Pfafferott, HJ Meiselman, P Hochstein; The effect of malonyldialdehyde on erythrocyte deformability. Blood 1982; 59 (1): 12- ... The addition of malonyldialdehyde (5-20 micro M) to human erythrocytes results in a marked decrease in cellular deformability ... At high shear forces (125-500 dynes/sq cm), malonyldialdehyde at 5 micro M had no effect on deformability, although at ...
The erythrocyte deformability is determined by measurement of passage time (reciprocal of deformability) of erythrocyte ... The hemorheological parameters, erythrocyte aggregation and deformability are determined in leprotic patients and are compared ... The observations show that in leprosy the aggregation of erythrocyte is marginally reduced and the deformability is ... Kumaravel M, Singh M. Aggregation and deformability of erythrocytes in leprosy. Indian Journal of Experimental Biology. 1995 ...
Deformability of Erythrocytes and Factors Affecting it From Childhood to Old Age: A Comparative Study , Author: MOHAMED T. EL- ... deformability). Group 1 erythrocytes were found to have the shortest filtration time (the best deformability) as compared to ... The aim of this study is to evaluate the effect of age and sex-related changes in deformability of human erythrocytes, as well ... Deformability of Erythrocytes and Factors Affecting it From Childhood to Old Age: A Comparative Study ...
1. Erythrocyte deformability studies by viscometry and filtrometry. Open this publication in new window or tab ,,Erythrocyte ... When incubating erythrocytes with calcium ions a more direct effect on erythrocyte deformability is seen. ... Erythrocyte deformability measured by filtrometry: Influences of variations in filtration pressure, MCV and MCHC. Persson, ... Erythrocyte deformability can be measured as filterability through a polycarbonate filter. To evaluate the influence of ...
Effects of iron overload and exenatide on erythrocyte deformability in a rat model ... that iron overload which directly affects the erythrocyte morphology may also interfere with erythrocyte deformability (ED). ... Iron overload is known to affect erythrocyte membrane properties and erythrocyte shape. We hypothesized ...
High-Intensity Interval Training Improves Erythrocyte Osmotic Deformability. HUANG, YU-CHIEH; HSU, CHIH-CHIN; WANG, JONG-SHYAN ...
The Effects of HES 130/0.4 Application on Erythrocyte Deformability in Ureteral Obstructed Rats ... However, the effects of hydroxyethyl starch (HES) on erythrocyte aggregation remain controversial. For this reason, we aimed to ...
The aim of this study was to evaluate the effect of dexmedetomidine on lipid peroxidation and erythrocyte deformability during ... Dexmedetomidine protects against lipid peroxidation and erythrocyte deformability alterations in experimental hepatic ischemia ...
el valle de dios document erythrocyte deformability pdf synonym fillable quote template kisspng deformabilit 5b48ec1d2 built to ... El Valle De Dios Document Erythrocyte Deformability Pdf Synonym Fillable Quote Template Kisspng Deformabilit 5b48ec1d2. Home〉 ... El valle de dios document erythrocyte deformability pdf synonym fillable quote template kisspng deformabilit 5b48ec1d2. View ...
Ligand mediated control of erythrocyte deformability for optimized delivery of hemostatic and antithrombotic biotherapeutics ... Therefore, engineering deformability via appended ligands is a particularly attractive approach to optimize the efficacy of ... such as membrane deformability, and how these changes interrelate with efficacy and pharmacodynamics. We recently described how ...
Effects of Hyul-Bu-Chuke-Tang on Erythrocyte Deformability and Cerebrovascular CO2 Reactivity in Normal Subjects. . Biblioteca ... We demonstrated that HCEt improved erythrocyte deformability and CVR. Our findings suggest that an improvement in erythrocyte ... We examined erythrocyte deformability in an HCEt group and a control group . CVR was measured using hyperventilation-induced ... HCEt significantly improved erythrocyte deformability 1 hour after administration compared to the control group % versus %, . ...
  • Erythrocyte deformability is an important determinant of blood viscosity, hence blood flow resistance in the vascular system. (wikipedia.org)
  • On fasting venous blood we evaluated whole-blood and plasma viscosity at high and low shear rates, haematocrit, the ratios between whole-blood viscosity (at high and low shear rate) and haematocrit×100, the ratio between plasma viscosity at low and high shear rate and the erythrocyte deformability examined by using laser diffractometry and expressed as elongation index. (iospress.com)
  • We discuss some hypotheses that might explain the behavior of red blood cell deformability in MM, considering that its impairment, in addition to the increase of plasma viscosity, can alter the microcirculatory flow in these patients. (iospress.com)
  • The deformability of normal and hardened erythrocytes (obtained by heating blood at 49°C or by incubating erythrocytes in a solution of hydrogen peroxide) was measured with a slit diffractometer and erythrocyte suspension viscosity was measured with a rotational viscometer. (elsevier.com)
  • We found that when erythrocytes were heated at 49°C, with much less deformability than normal erythrocytes, their suspension viscosity was almost the same as the normal blood viscosity at high shear rates, whereas when erythrocytes were incubated in a solution of hydrogen peroxide, with an intermediate decrease of deformability, their suspension viscosity was greatly increased. (elsevier.com)
  • The present study concluded that not all rigid cells cause an increase in blood viscosity at high shear rates, and therefore that decreased cell deformability is not always a good indicator of high-shear blood viscosity. (elsevier.com)
  • Zhang, J, Johnson, PC & Popel, AS 2009, ' Effects of erythrocyte deformability and aggregation on the cell free layer and apparent viscosity of microscopic blood flows ', Microvascular Research , vol. 77, no. 3, pp. 265-272. (elsevier.com)
  • All cases were subjected to the following laboratory investigations: Haemoglobin estimation, total red cell count, haematocrit value, erythrocyte sedimentation rate, whole blood viscosity, plasma viscosity, packed cell viscosity (PCV), plasma lipid estimation, red cell membrane lipid content and erythrocyte filtrability (deformability). (bu.edu.eg)
  • As whole blood viscosity has been found to be a factor constantly affecting deformability, its measurement might help to prevent vascular risks in disease states. (bu.edu.eg)
  • RBC deformability is determined by cells internal viscosity and cytoskeleton structure. (ac.ru)
  • The aim of the present study was to test the effects of one year of HU therapy on blood rheology (RBC deformability and aggregation, blood viscosity), hematology and clinical profile in SCA adults. (haematologica.org)
  • We demonstrated that the improvement in RBC deformability and the decrease of RBC aggregates robustness in HU-treated patients limit the consequences of the increase of hemoglobin on blood viscosity. (haematologica.org)
  • The primary determinants of blood viscosity are hematocrit, red blood cell deformability, red blood cell aggregation, and plasma viscosity. (wikipedia.org)
  • Exposure of normal erythrocytes to super-physiologic but subhemolytic shear stresses results in decreased red cell deformability. (rice.edu)
  • The significance of cellular deformability is readily apparent based solely on the disparate mean size of human erythrocytes (~8 μm) and leukocytes (10-25 μm) compared to the minimum luminal size of capillaries (4-5 μm) and splenic interendothelial clefts (0.5-1.0 μm) they must transit. (intechopen.com)
  • The addition of malonyldialdehyde (5-20 micro M) to human erythrocytes results in a marked decrease in cellular deformability as measured with a counter-rotating, cone plate Rheoscope when low shear forces (2.5-25 dynes/sq cm) are applied. (ashpublications.org)
  • The aim of this study is to evaluate the effect of age and sex-related changes in deformability of human erythrocytes, as well as other haemorrheologic parameters affecting it. (bu.edu.eg)
  • The present thesis concerns factors of major importance in filtrometric studies of human erythrocytes, e.g. influence of buffer media, importance of filtration pressure for filtration through micropores and possible effects of damaged and hemolyzed blood cells. (diva-portal.org)
  • C3b deposition on human erythrocytes induces the formation of a membrane skeleton-linked protein complex. (nih.gov)
  • Human erythrocytes are highly specialized enucleate cells that are involved in providing efficient gas transport. (wpinstitute.org)
  • When human erythrocytes are invaded by the malaria parasite Plasmodium falciparum , selected DRM proteins (major proteins flotillin-1 and flotillin-2, and at least 13 additional proteins) are exclusively internalized into the newly formed PVM. (bloodjournal.org)
  • The feasibility of accurately determining the elastic properties of biological cells with the optical stretcher was demonstrated by stretching human erythrocytes. (utexas.edu)
  • It is suggested that there is a role for oxidative stress in the pathophysiology of many diseases, one of them being OP. In this study, we investigated the effect of orchiectomy on bone mineral density (BMD), the level of antioxidant enzymes, lipid peroxidation, and erythrocyte deformability in rats and the effect of vitamin C supplementation on the possible changes in these parameters. (ftrdergisi.com)
  • and (3) the effects of RT on cortical/movement reorganization as well as on the physiological markers of inflammation, oxidative stress, and erythrocyte deformability. (strokecenter.org)
  • Blood samples of the patients (12 ml.) will be collected to examine the physiological markers (ie, inflammation-related markers, oxidative stress markers, and erythrocyte deformability) before and after intervention. (strokecenter.org)
  • Here, we demonstrate that the measurement of changes in RBC deformability is a sensitive method for inferring heme-induced oxidative stress. (rsc.org)
  • IMSEAR at SEARO: Aggregation and deformability of erythrocytes in leprosy. (who.int)
  • Kumaravel M, Singh M. Aggregation and deformability of erythrocytes in leprosy. (who.int)
  • The hemorheological parameters, erythrocyte aggregation and deformability are determined in leprotic patients and are compared with that of healthy subjects. (who.int)
  • Erythrocyte: A systems model of the control of aggregation and deformability. (wpinstitute.org)
  • abstract = "This paper presents a novel method for an on-chip erythrocyte deformability test under optical pressure, especially to enhance the level of sensitivity with respect to the detection of cancerous diseases. (elsevier.com)
  • Deferiprone (Ll) treatment removes erythrocytes membrane free iron and affects K-Cl cotransport activity in homozygous b thalassemia [abstract]. (semanticscholar.org)
  • Concentrated erythrocyte (i.e., red blood cell) suspensions flowing in microchannels have been simulated with an immersed-boundary lattice Boltzmann algorithm, to examine the cell layer development process and the effects of cell deformability and aggregation on hemodynamic and hemorheological behaviors. (elsevier.com)
  • In line with the preparation of the new Guidelines for the standardization of hemorheological measurement, we compared various blood rheological parameters (i.e., red blood cell deformability and aggregation indices) assessed in blood samples obtained after 5, 30, 60 and 90 s following the tourniquet removal and a blood sample obtained without applying a tourniquet (control sample). (bezmialem.edu.tr)
  • Sickle-cell disease is characterized by extensive impairment in erythrocyte deformability, being dependent on the oxygen partial pressure. (wikipedia.org)
  • However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. (koreamed.org)
  • Significant impairment in erythrocyte deformability developed during ANH. (physiology.org)
  • This is an important property because erythrocytes must change their shape extensively under the influence of mechanical forces in fluid flow or while passing through microcirculation. (wikipedia.org)
  • INTRODUCTION: Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. (koreamed.org)
  • Our findings suggest that an improvement in erythrocyte deformability contributes to HCEt's effect on cerebral microcirculation. (duhnnae.com)
  • Erythrocyte rheology and the optimization of mass transport in the microcirculation. (springer.com)
  • The membrane protein on the surface of erythrocyte membrane enables erythrocyte to have plasticity and pass through the microcirculation without being blocked or destroyed. (portlandpress.com)
  • The latter may be due to the impaired ability of erythrocytes to off-load oxygen within the microcirculation, thus yielding low tissue/plasma P o 2 but elevated microcirculatory hemoglobin oxygen (HbO 2 ) saturations. (physiology.org)
  • METHODS: Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. (koreamed.org)
  • The erythrocyte deformability is determined by measurement of passage time (reciprocal of deformability) of erythrocyte suspension in PBS at hematocrit 6% through cellulose membrane. (who.int)
  • Erythrocyte deformability was measured using erythrocyte suspensions at 5% hematocrit in phosphate buffered saline. (ftrdergisi.com)
  • It can increase in parallel with erythrocyte sedimentation rate (ESR), but it is not influenced by hematocrit (anemia, polycytemia), or time to analysis. (nih.gov)
  • After erythropoiesis had returned hematocrit to normal, treated and control fish were subjected to a seasonal cold acclimation regime to assess the impact of erythrocyte age on skeletal muscle remodeling. (biologists.org)
  • Alterations in membrane deformability in hemolytic anemias. (springer.com)
  • Although much attention has been directed toward the enhancement of the circulation time of particles, including alterations of particle size ( 5 , 6 ), shape ( 7 - 9 ), and surface characteristics ( 10 ), the role played by deformability in the in vivo circulation profile of particles has not been well explored. (pnas.org)
  • For example, perfusion is more sensitive to relatively small changes in deformability (compared to filterability), thus making it preferable for assessing RBC deformability in contexts where microcirculatory implications are of particular interest. (wikipedia.org)
  • Erythrocyte deformability can be measured as filterability through a polycarbonate filter. (diva-portal.org)
  • A significant decrease in erythrocyte deformability, especially at low shear stresses, was found. (iospress.com)
  • The decrease of deformability at a pH of 6.8 was most pronounced in the splenectomized patients, and likewise in erythrocytes from the splenic artery, which were obtained from one patient during splenectomy. (mysciencework.com)
  • At high shear forces (125-500 dynes/sq cm), malonyldialdehyde at 5 micro M had no effect on deformability, although at concentrations of 10 and 20 micro M a small but statistically significant decrease was evident. (ashpublications.org)
  • One of the striking features of this parasite is its ability to remodel and decrease the deformability of host red blood cells, a process that contributes to disease. (edu.au)
  • On the contrary, no correlation between deformability and either ESR or RBCs membrane lipid in any studied group was found. (bu.edu.eg)
  • The aim of this study was to evaluate the effect of dexmedetomidine on lipid peroxidation and erythrocyte deformability during ischemia-reperfusion injury in rats. (ksbu.edu.tr)
  • Lipid mass spectrometry revealed that phosphatidylethanolamine and phosphatidylglycerol are depleted in endovesicles while phosphoinositides are highly enriched, suggesting raft-based endovesiculation can be achieved by simple (non-receptor-mediated) mechanical perturbation of the erythrocyte plasma membrane and results in sorting of inner leaflet phospholipids. (bloodjournal.org)
  • Live-cell imaging of lipid-specific protein probes showed that phosphatidylinositol (4,5) bisphosphate (PIP 2 ) is highly concentrated in primaquine-induced vesicles, confirming that it is an erythrocyte raft lipid. (bloodjournal.org)
  • Degradation of deformability of erythrocytes under various types of oxygen deficit deteriorates functioning of the system of oxygen transportation at various levels: heart, vascular flow, oxygen-transporting blood function. (mysciencework.com)
  • Under hypoxia the parameters of oxygen-transporting blood function, of peroxide oxidation of lipids and of the antioxidation system correlate well with degradation of deformability of erythrocytes. (mysciencework.com)
  • Deformability of erythrocytes is a factor generating adequate the oxygen supply to tissue, and its degradation aggravates substitution of the oxidase pattern of oxygen utilization with the oxygenase pattern. (mysciencework.com)
  • In an ektacytometer erythrocyte deformability can be measured under changing osmotic stress or oxygen tension and is used in the diagnosis and follow up of congenital hemolytic anemias. (wikipedia.org)
  • Mature erythrocytes are non-nucleated, biconcave disks containing Hemoglobin whose function is to transport Oxygen . (jove.com)
  • Diamide decreases deformability of rabbit erythrocytes and attenuates low oxygen tension-induced ATP release. (thefreedictionary.com)
  • Therefore, this research collected 30 cases of healthy blood donors, and explored erythrocyte stored at different times relating indicators including effective oxygen uptake (Q), P50, 2,3-DPG, Na + -k + -ATP. (portlandpress.com)
  • To explore the effective carry oxygen capacity of erythrocyte at different storage time resulting in the expression change of erythrocyte surface membrane protein. (portlandpress.com)
  • Alternatively, oxygen shunting may also be due to reduced erythrocyte deformability, hindering the ability of erythrocytes to enter capillaries. (physiology.org)
  • This shunting appears to be not primarily caused by oxygen off-load deficit but rather by oxygen/erythrocytes bypassing capillaries, wherein a potential contributor is impaired erythrocyte deformability. (physiology.org)
  • Deformability is an intrinsic cellular property of erythrocytes determined by geometric and material properties of the cell membrane, although as with many measurable properties the ambient conditions may also be relevant factors in any given measurement. (wikipedia.org)
  • We show here that mature GIEs are more deformable than immature stages using ektacytometry and microsphiltration methods, and that a switch in cellular deformability in the transition from immature to mature gametocytes is accompanied by the deassociation of parasite-derived STEVOR proteins from the infected erythrocyte membrane. (pasteur.fr)
  • PDF] The effect of dietary magnesium supplementation on the cellular abnormalities of erythrocytes in patients with beta thalassemia intermedia. (semanticscholar.org)
  • Thalassaemic erythrocytes: cellular suicide arising from iron and glutathione-dependent oxidation reactions? (semanticscholar.org)
  • 4 Host cholesterol is also detected in the PVM, and depletion of erythrocyte cholesterol (which disrupts DRM complexes but has no effect on membrane deformation or cellular ATP levels) blocks infection. (bloodjournal.org)
  • This optical deformability was exploited for the measurement of cellular elasticities with a novel micromanipulation device based on a two-beam fiber-based laser trap, the optical stretcher, which can generate surface forces from 1 pN-1 nN at frequencies ranging from static experiments to several MHz. (utexas.edu)
  • We hypothesize that mechanical retention contributes to sequestration of immature GIEs and that regained deformability of mature gametocytes is associated with their release in the bloodstream and ability to circulate. (pasteur.fr)
  • Beside endothelial cells, red blood cells (RBCs) possess NOS and produce nitric oxide (NO), which contributes to RBC deformability. (dovepress.com)
  • The formation of Plasmodium falciparum gametocytes in the human host takes several days during which immature gametocyte-infected erythrocytes (GIEs) sequester in host tissues. (pasteur.fr)
  • OPUS at UTS: A Plasmodium falciparum S33 proline aminopeptidase is associated with changes in erythrocyte deformability. (edu.au)
  • A Plasmodium falciparum S33 proline aminopeptidase is associated with changes in erythrocyte deformability. (edu.au)
  • Studies of detergent-resistant membrane (DRM) rafts in mature erythrocytes have facilitated identification of proteins that regulate formation of endovacuolar structures such as the parasitophorous vacuolar membrane (PVM) induced by the malaria parasite Plasmodium falciparum . (bloodjournal.org)
  • Erythrocyte deformability refers to the ability of erythrocytes (red blood cells, RBC) to change shape under a given level of applied stress, without hemolysing (rupturing). (wikipedia.org)
  • we considered the possibility that each aspect of cold-induced muscle remodeling (cardiac hypertrophy, skeletal muscle angiogenesis, mitochondrial proliferation) could be attributed to changes in hemodynamics, such as the ability of erythrocytes to penetrate the peripheral vasculature. (biologists.org)
  • The suspension of hardened erythrocytes (red blood cells) differs from the suspension of normal erythrocytes with respect to their rheological behavior. (elsevier.com)
  • Red blood cells have unique mechanical behavior, which can be discussed under the terms erythrocyte deformability and erythrocyte aggregation. (wikipedia.org)
  • Further, we expect these results will stand as an introduction for particle deformability as a vital design parameter that can affect the behavior of particles on both the micro- and nanoscales. (pnas.org)
  • It also affects the microcirculatory blood flow significantly, where erythrocytes are forced to pass through blood vessels with diameters smaller than their size. (wikipedia.org)
  • R. glutinosa prevented decreases in erythrocyte deformability in rats with endotoxin-induced disseminated intravascular coagulation. (drugs.com)
  • In normal rats, R. glutinosa enhanced or increased erythrocyte deformability and fibrinolytic activity. (drugs.com)
  • Thus, by two different approaches we confirm that the drop in cAMP levels in mature gametocytes leads to an increase in their deformability and hence more likely to circulate through the spleen. (prolekare.cz)
  • These findings imply that deformability is indeed an important pathophysiological quantity. (rice.edu)
  • These findings provide the proof of principle that deformability of circulating gametocytes is targetable by pharmacological agents and as such, it provides a novel approach to prevent the spread of parasites. (prolekare.cz)
  • DISCUSSION: These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage. (koreamed.org)
  • Therefore, we examined osmotic RBC deformability over a range of shear stresses (0.5-20 Pa). (iospress.com)
  • Finally, we show that reduced erythrocyte deformability corresponds with both erythrophagocytosis and RBC osmotic fragility. (rsc.org)
  • In this work, cell margination is mimicked for the separation of i RBCs from whole blood based on their reduced deformability. (rsc.org)
  • Regarding sex-related differences in deformability, it was not found in group I while it was clear in other groups, where males RBCs were less deformable than those of females. (bu.edu.eg)
  • Due to a special phenomenon called tank treading, the membrane of the erythrocyte (red blood cell, RBC) rotates relative to the shear force and the cell's cytoplasm causing RBCs to orient themselves. (wikipedia.org)
  • Splenic sequestration of these abnormal RBCs is the dominant cause of decreased erythrocyte survival in hereditary elliptocytosis. (medscape.com)
  • The present study tested the capacity of statins to activate NOS in RBCs and subsequently to modulate RBC deformability in vitro. (dovepress.com)
  • Red cell deformability changes have been evaluated in several types of hemolytic anemias using a new diffractometric technique (ektacytometer). (springer.com)
  • These results confirm the earlier reports of reduced deformability of red cells in various hemolytic anemias and provide additional quantitative data on the presence of subpopulations of undeformable cells. (springer.com)
  • reflecting the cytoplasmic hemoglobin concentration of erythrocytes. (wikipedia.org)
  • Hemoglobin CC disease: Rheological properties of erythrocytes and abnormalities in cell water. (springer.com)
  • Production of erythrocytes that contain fetal hemoglobin in anemia. (biomedsearch.com)
  • Serial microscopic immunodiffusion assays of F cells, i.e., erythrocytes that contain fetal hemoglobin (HbF), in four individuals recovering from anemia demonstrate initial increases in the percentage of circulating reticulocytes that contain HbF (F reticulocytes) and subsequent increases in the percentage of mature erythrocytes that contain HbF (F erythrocytes). (biomedsearch.com)
  • We hypothesized that iron overload which directly affects the erythrocyte morphology may also interfere with erythrocyte deformability (ED). Exenatide, a glucagon like peptide -1 (GLP-1) analogue used in the treatment of diabetes, is known to have beneficial pleiotropic effects on endothelial function and blood flow which are different from its glucose-lowering effects. (ksbu.edu.tr)
  • Syk inhibitors interfere with erythrocyte membrane modification during P falciparum growth and suppress parasite egress. (purdue.edu)
  • During this period, immature gametocyte-infected erythrocytes (GIE) sequester in internal organs such as bone marrow and spleen [ 3 - 6 ]. (prolekare.cz)
  • Bagge U. (1978) Intravascular Rheology of Erythrocytes in Man. (springer.com)
  • Hereditary elliptocytosis and hereditary pyropoikilocytosis are congenital hemolytic disorders in which erythrocytes either are elongated into an oval form or are irregularly shaped (see images below). (medscape.com)
  • Cigar-shaped erythrocytes seen in hereditary elliptocytosis. (medscape.com)
  • Hereditary elliptocytosis and its related disorders are caused by mutations that disrupt the RBC cytoskeleton, a multiprotein complex responsible for the elasticity and durability of the circulating erythrocytes. (medscape.com)
  • In hereditary elliptocytosis, circulating erythrocytes undergo a progressive transformation from a normal discocyte to an elliptocyte. (medscape.com)
  • Erythrocytes in hereditary elliptocytosis lack elasticity and remain elliptical. (medscape.com)
  • Erythrocytes from hereditary xerocytosis patients heterozygous for KCNN4 V282M exhibit increased spontaneous Gardos channel-like activity inhibited by senicapoc. (childrenshospital.org)
  • Erythrocyte deformability was measured using a laser diffractoscope in the shear stress range 0.3-60 Pa. (hindawi.com)
  • Erythrocyte deformability was measured as shear stress-induced cell elongation (LORCA difractometer). (physiology.org)
  • Thus for meaningful "apples-to-apples" comparisons involving cell deformability, it is important to utilize the same qualitative approach. (wikipedia.org)
  • Changes in the deformability of either cell will result in their premature mechanical clearance as well as an enhanced possibility of intravascular lysis. (intechopen.com)
  • Erythrocyte deformability for this work is determined by the pressure drop during constant volumetric flowrate filtration of a red cell suspension through the 3 (mu)m diameter pores of a Nuclepore filter. (rice.edu)
  • Deformability of erythrocytes from four patients with different types of glucosephosphate isomerase (D-glucose-6-phosphate ketoisomerase, GPI) deficiency has been determined by cell filtration. (mysciencework.com)
  • This pilot study aimed to evaluate the differential effects of a remote ischemic preconditioning (rIPC) manoeuvre on performance and red blood cell (RBC) deformability compared to a sham control and a placebo setting. (dshs-koeln.de)
  • We found that the cell free layer thickness increases with both cell deformability and aggregation strength. (elsevier.com)
  • A slight but significant improvement in red blood cell (RBC) deformability after the removal of tourniquet compared to the control sample was observed. (bezmialem.edu.tr)
  • In addition genetic deletion of this exopeptidase was shown to lead to an increase in the deformability of parasite-infected red cells and in reduced adherence to the endothelial cell receptor CD36 under flow conditions. (edu.au)
  • Oral magnesium supplements reduce erythrocyte dehydration in patients with sickle cell disease. (semanticscholar.org)
  • Therapy with oral clotrimazole induces inhibition of the Gardos channel and reduction of erythrocyte dehydration in patients with sickle cell disease. (semanticscholar.org)
  • Without a nucleus, a mature erythrocyte (or RBC) contains a cytosol enclosed within a highly flexible cell membrane. (frontiersin.org)
  • The mature erythrocyte is a terminally differentiated, nonendocytic cell in nature. (bloodjournal.org)
  • We have previously developed the multiplex fluidic plunger (MFP) device for the measurement of red blood cell (RBC) deformability. (rsc.org)
  • We further show that extracellular hemin concentration correlates closely with changes in RBC deformability and we confirm that this biophysical change correlates with other indicators of cell stress. (rsc.org)
  • A 50% ethanolic extract of R. glutinosa inhibited decreases in fibrinolytic activity and erythrocyte deformability, decreases in erythrocyte counts, and increases in connective tissue in rodents. (drugs.com)
  • Erythrocyte deformability is altered under various pathophysiological conditions. (wikipedia.org)
  • Acidic environment may enhance membran rigidity in vitro and also during passage of the erythrocytes through the spleen. (mysciencework.com)
  • Studies of Erythrocyte Membrane Loss Produced by Amphipathic Drugs and in Vitro Storage Greenwalt, Tibor J. (deepdyve.com)
  • We implemented laser ektacytometer RheoScan AnD-300 to assess changes of deformability of RBC extracted from heparinized blood at presence of forskolin - the direct stimulator of adenylyl cyclase. (ac.ru)
  • Column technique : A way to assess erythrocyte deforambility? (lnu.se)
  • C-peptide, penta- and hexapeptide all significantly improved the impaired erythrocyte deformability of type 1 diabetic patients, while the middle fragment and scrambled C-peptide had no detectable effect. (hindawi.com)
  • The clinical improvement of all patients following splenectomy which is accompanied by an increase of the erythrocyte survival time and by unchanged reticulocyte counts, is in accordance with this view. (mysciencework.com)
  • We have therefore studied the effect of dietary Mg supplementation in patients with beta thalassemia intermedia in order to establish whether it improves the abnormalities of thalassemic erythrocytes. (semanticscholar.org)
  • To demonstrate the performance and sensitivity of the combined method, we introduce the concept of transit velocity, a modified elongation index, and shape recovery time of individual erythrocytes in a strictly confined region (2 μm deep, 4 μm wide, and 100 μm long). (elsevier.com)
  • Non-deformable immature gametocyte stages are sequestered in the bone marrow, as their lack of deformability would lead to their splenic clearance. (prolekare.cz)
  • In the present study, we apply nano-filtration technology to mimic splenic retention and demonstrate that deformability of transmissible mature stage V gametocytes is regulated by parasite cyclic AMP-dependent kinase signalling. (prolekare.cz)
  • It is suggested that the metabolic environment of the spleen, with its low pH, impairs the deformability of GPI-deficient erythrocytes and predisposes them to splenic sequestration. (mysciencework.com)
  • Analysis of published and author's data on the physiological role of deformability of erythrocytes, general mechanisms of modifications and disturbances, assessment methods, and the role of this blood feature for assessing the body status. (mysciencework.com)
  • Understanding molecular mechanisms of the RBC deformability regulation according to the metabolic demands of the organism is an important task required for an effective control of the blood microrheology. (ac.ru)
  • Deformability is an essential intrinsic property of erythrocytes (red blood cells, RBC) which allows them to reversibly change shape and linear sizes and realize the gas transport function. (ac.ru)
  • Correlating deformability with the other studied parameters, positive correlations were found in: groups 11. (bu.edu.eg)
  • Malaria transmission is ensured by deformable mature gametocyte-infected erythrocytes being taken up when a mosquito bites. (prolekare.cz)
  • Importantly, erythrocyte raft lipids recruited to the invasion junction by mechanical stimulation may be remodeled by the malaria parasite to establish blood-stage infection. (bloodjournal.org)
  • Normal erythrocytes, however, regain their discoid shape. (medscape.com)
  • Group 1 erythrocytes were found to have the shortest filtration time (the best deformability) as compared to older groups II, III and IV, the difference was statistically highly significant, meanwhile no statistical difference in deformability was found between groups II, HI and IV. (bu.edu.eg)
  • Young as well as whole erythrocyte populations had a markedly increased rigidity and an abnormally strong attachment of haemoglobin to the inner surface of isolated membranes. (mysciencework.com)
  • Recently, we and others have shown that erythrocytes contain detergent-resistant membranes (DRMs). (bloodjournal.org)
  • Iron overload is known to affect erythrocyte membrane properties and erythrocyte shape. (ksbu.edu.tr)
  • A change in the deformability of the erythrocytes, due to any internal or environmental factor, affects the rotation speed of the trapped crenated cells. (iisc.ac.in)
  • Intracellular calcium, as measured by a modification of Harrison and Long's procedure, is increased by 35 and 55% above control levels following exposure to shear stresses of 1000 and 1300 dynes/cm('2) for two minutes, and is associated with decreasing deformability. (rice.edu)
  • Ligation of complement receptor 1 increases erythrocyte membrane deformability. (nih.gov)
  • Oriented and stretched red blood cells have a diffraction pattern representing the apparent particle size in each direction, making it possible to measure the erythrocyte deformability and the orientability of the cells. (wikipedia.org)
  • In conclusion, this study revealed that RBC deformability and aggregation might be significantly altered in the samples obtained after the application and removal of a tourniquet, as a part of the blood sampling procedure. (bezmialem.edu.tr)
  • Here, we use primaquine to perturb the erythrocyte membrane and induce detergent-free buoyant vesicles, which are enriched in cholesterol and major raft proteins flotillin and stomatin and contain low levels of cytoskeleton, all characteristics of raft microdomains. (bloodjournal.org)
  • In this chapter, we will demonstrate how microfluidic devices can be used to examine the vascular deformability of erythrocytes and agranular leukocytes. (intechopen.com)