Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
IMMUNOGLOBULINS on the surface of B-LYMPHOCYTES. Their MESSENGER RNA contains an EXON with a membrane spanning sequence, producing immunoglobulins in the form of type I transmembrane proteins as opposed to secreted immunoglobulins (ANTIBODIES) which do not contain the membrane spanning segment.
Sites on an antigen that interact with specific antibodies.
Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.
Antigenic determinants recognized and bound by the T-cell receptor. Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen.
The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions.
A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A genus of the family HERPESVIRIDAE, subfamily ALPHAHERPESVIRINAE, consisting of herpes simplex-like viruses. The type species is HERPESVIRUS 1, HUMAN.
A glycoprotein that is a kallikrein-like serine proteinase and an esterase, produced by epithelial cells of both normal and malignant prostate tissue. It is an important marker for the diagnosis of prostate cancer.
Polymorphic class I human histocompatibility (HLA) surface antigens present on almost all nucleated cells. At least 20 antigens have been identified which are encoded by the A locus of multiple alleles on chromosome 6. They serve as targets for T-cell cytolytic responses and are involved with acceptance or rejection of tissue/organ grafts.
A gland in males that surrounds the neck of the URINARY BLADDER and the URETHRA. It secretes a substance that liquefies coagulated semen. It is situated in the pelvic cavity behind the lower part of the PUBIC SYMPHYSIS, above the deep layer of the triangular ligament, and rests upon the RECTUM.
Subunits of the antigenic determinant that are most easily recognized by the immune system and thus most influence the specificity of the induced antibody.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.
An acute infection caused by the spore-forming bacteria BACILLUS ANTHRACIS. It commonly affects hoofed animals such as sheep and goats. Infection in humans often involves the skin (cutaneous anthrax), the lungs (inhalation anthrax), or the gastrointestinal tract. Anthrax is not contagious and can be treated with antibiotics.
A compound with many biomedical applications: as a gastric antacid, an antiperspirant, in dentifrices, as an emulsifier, as an adjuvant in bacterins and vaccines, in water purification, etc.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
A species of gram-negative, aerobic bacteria that is the causative agent of WHOOPING COUGH. Its cells are minute coccobacilli that are surrounded by a slime sheath.
A species of the PESTIVIRUS genus causing exceedingly contagious and fatal hemorrhagic disease of swine.
Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.
An acute, highly contagious disease affecting swine of all ages and caused by the CLASSICAL SWINE FEVER VIRUS. It has a sudden onset with high morbidity and mortality.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Membrane glycoproteins consisting of an alpha subunit and a BETA 2-MICROGLOBULIN beta subunit. In humans, highly polymorphic genes on CHROMOSOME 6 encode the alpha subunits of class I antigens and play an important role in determining the serological specificity of the surface antigen. Class I antigens are found on most nucleated cells and are generally detected by their reactivity with alloantisera. These antigens are recognized during GRAFT REJECTION and restrict cell-mediated lysis of virus-infected cells.
Vaccines or candidate vaccines containing inactivated HIV or some of its component antigens and designed to prevent or treat AIDS. Some vaccines containing antigens are recombinantly produced.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.
A malignant epithelial tumor with a glandular organization.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A species of LENTIVIRUS, subgenus equine lentiviruses (LENTIVIRUSES, EQUINE), causing acute and chronic infection in horses. It is transmitted mechanically by biting flies, mosquitoes, and midges, and iatrogenically through unsterilized equipment. Chronic infection often consists of acute episodes with remissions.
Viral disease of horses caused by the equine infectious anemia virus (EIAV; INFECTIOUS ANEMIA VIRUS, EQUINE). It is characterized by intermittent fever, weakness, and anemia. Chronic infection consists of acute episodes with remissions.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Subpopulation of CD4+ lymphocytes that cooperate with other lymphocytes (either T or B) to initiate a variety of immune functions. For example, helper-inducer T-cells cooperate with B-cells to produce antibodies to thymus-dependent antigens and with other subpopulations of T-cells to initiate a variety of cell-mediated immune functions.
A publication issued at stated, more or less regular, intervals.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Polyomavirus antigens which cause infection and cellular transformation. The large T antigen is necessary for the initiation of viral DNA synthesis, repression of transcription of the early region and is responsible in conjunction with the middle T antigen for the transformation of primary cells. Small T antigen is necessary for the completion of the productive infection cycle.
A component of the murine major histocompatibility complex class I family. It contains one Ig-like C1-type domain and functions in processing and presentation of exogenous peptide antigens to the immune system.
Proteins conjugated with nucleic acids.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)

Crystal structure of an MHC class I presented glycopeptide that generates carbohydrate-specific CTL. (1/4245)

T cell receptor (TCR) recognition of nonpeptidic and modified peptide antigens has been recently uncovered but is still poorly understood. Immunization with an H-2Kb-restricted glycopeptide RGY8-6H-Gal2 generates a population of cytotoxic T cells that express both alpha/beta TCR, specific for glycopeptide, and gamma/delta TCR, specific for the disaccharide, even on glycolipids. The crystal structure of Kb/RGY8-6H-Gal2 now demonstrates that the peptide and H-2Kb structures are unaffected by the peptide glycosylation, but the central region of the putative TCR binding site is dominated by the extensive exposure of the tethered carbohydrate. These features of the Kb/RGY8-6H-Gal2 structure are consistent with the individual ligand binding preferences identified for the alpha/beta and gamma/delta TCRs and thus explain the generation of a carbohydrate-specific T cell response.  (+info)

Crystal structures of two H-2Db/glycopeptide complexes suggest a molecular basis for CTL cross-reactivity. (2/4245)

Two synthetic O-GlcNAc-bearing peptides that elicit H-2Db-restricted glycopeptide-specific cytotoxic T cells (CTL) have been shown to display nonreciprocal patterns of cross-reactivity. Here, we present the crystal structures of the H-2Db glycopeptide complexes to 2.85 A resolution or better. In both cases, the glycan is solvent exposed and available for direct recognition by the T cell receptor (TCR). We have modeled the complex formed between the MHC-glycopeptide complexes and their respective TCRs, showing that a single saccharide residue can be accommodated in the standard TCR-MHC geometry. The models also reveal a possible molecular basis for the observed cross-reactivity patterns of the CTL clones, which appear to be influenced by the length of the CDR3 loop and the nature of the immunizing ligand.  (+info)

Phenotypic and functional characterization of CD8(+) T cell clones specific for a mouse cytomegalovirus epitope. (3/4245)

A series of CD8(+) T cell clones, specific for the IE1 epitope YPHFMPTNL, of the immediate-early protein 1 of the murine cytomegalovirus (MCMV) were generated in order to determine their protective activity against this infection and correlate their phenotypic markers with antiviral activity. We found that the adoptive transfer of three of these anti-MCMV CD8(+) T cell clones into irradiated naive mice resulted in protection against challenge, while another CD8(+) T cell clone, of the same specificity, failed to confer protection. The clones that conferred protection against lethal challenge reduced greatly viral replication in the lung and other organs of the mice. Using one of the protective anti-MCMV CD8(+) T cell clones we found that in order to be fully protective the cells had to be transferred to recipient mice no later than 1 day after MCMV challenge. The adoptive transfer of these CD8(+) T cell clones also protected CD4(+) T-cell-depleted mice. Phenotypic characterization of the anti-MCMV clones revealed that the nonprotective clone expressed very low levels of CD8 molecules and produced only small amounts of TNF-alpha upon antigenic stimulation. Most importantly, our current study demonstrates that this MHC class I-restricted IE1 epitope of MCMV is efficiently presented to CD8(+) T cell clones in vivo and further strengthens the possibility of the potential use of CD8(+) T cell clones as immunotherapeutic tools against cytomegalovirus-induced disease.  (+info)

Immune response to the immunodominant epitope of mouse hepatitis virus is polyclonal, but functionally monospecific in C57Bl/6 mice. (4/4245)

Mutations in an immunodominant CD8 CTL epitope (S-510-518) are selected in mice persistently infected with the neurotropic JHM strain of mouse hepatitis virus. These mutations abrogate recognition by T cells harvested from the infected CNS in direct ex vivo cytotoxicity assays. Previous reports have suggested that, in general, an oligoclonal, monospecific T cell response contributes to the selection of CTL escape mutants. Herein, we show that, in MHV-JHM-infected mice, the CD8 T cell response after intraperitoneal infection is polyclonal and diverse. This diverse response was shown to include both polyclonal and oligoclonal components. The polyclonal data were shown to fit a logarithmic distribution. With regard to specificity, we used a panel of peptide analogues of epitope S-510-518 and spleen-derived CD8 T cell lines to determine why only a subset of possible mutations was selected in persistently infected mice. At a given position in the epitope, the mutations identified in in vivo isolates were among those that resulted in the greatest loss of recognition. However, not all such mutations were selected, suggesting that additional factors must contribute to selection in vivo. By extrapolation of these results to the persistently infected CNS, they suggest that the selection of CTL escape mutants requires the presence of a monospecific T cell response but also show that this response need not be oligoclonal.  (+info)

Induction of CD8+ T cell-mediated protective immunity against Trypanosoma cruzi. (5/4245)

Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circum-sporozoite (CS) protein, which contains the well-characterized CD8+ T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8+ T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8+ T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8+ T cells is effective against T. cruzi infection.  (+info)

In vivo and in vitro activation of T cells after administration of Ag-negative heat shock proteins. (6/4245)

Heat shock proteins (HSP) Hsp70 and gp96 prime class I-restricted cytotoxic T cells against Ags present in the cells from which they were isolated. The immunization capacity of HSPs is believed to rely on their ability to bind antigenic peptides. In this study, we employed the well-established OVA and beta-galactosidase (beta-gal) antigenic model systems. We show that in vitro long-term established OVA and beta-gal-specific CTL clones release TNF-alpha and IFN-gamma when incubated with Ag-negative Hsp70 and gp96. In the absence of antigenic peptides, HSP-mediated secretion of TNF-alpha and IFN-gamma requires cell contact of the APC with the T cell but is not MHC-I restricted. Moreover, Hsp70 molecules purified from Ag-negative tissue, e.g., negative for antigenic peptide, are able to activate T cells in vivo, leading to significant higher frequencies in OVA-specific CD8+ T cells. In unprimed animals, these T cells lyse OVA-transfected cell lines and produce TNF-alpha and IFN-gamma after Ag stimulus. Taken together our data show that, besides the well-established HSP/peptide-specific CTL induction and activation, a second mechanism exists by which Hsp70 and gp96 molecules activate T cells in vivo and in vitro.  (+info)

Fas-mediated suicide of tumor-reactive T cells following activation by specific tumor: selective rescue by caspase inhibition. (7/4245)

CD8+ T lymphocytes that specifically recognize tumor cells can be isolated and expanded ex vivo. While the lytic properties of these cells have been well described, their fate upon encounter with cognate tumor is not known. We performed reverse 51Cr release assays in which the lymphocyte effectors rather than the tumor cell targets were radioactively labeled. We found that melanoma tumor cells caused the apoptotic death of tumor-specific T cells only upon specific MHC class I-restricted recognition. This death was entirely blockable by the addition of an Ab directed against the Fas death receptor (APO-1, CD95). Contrary to the prevailing view that tumor cells cause the death of anti-tumor T cells by expressing Fas ligand (FasL), our data suggested that FasL was instead expressed by T lymphocytes upon activation. While the tumor cells did not express FasL by any measure (including RT-PCR), functional FasL (as well as FasL mRNA) was consistently found on activated anti-tumor T cells. We could successfully block the activation-induced cell death with z-VAD-fmk, a tripeptide inhibitor of IL-1 beta-converting enzyme homologues, or with anti-Fas mAbs. Most importantly, these interventions did not inhibit T cell recognition as measured by IFN-gamma release, nor did they adversely affect the specific lysis of tumor cell targets. These results imply that Fas-mediated activation-induced cell death could be a limiting factor in the in vivo efficacy of adoptive transfer of class I-restricted CD8+ T cells and provide a means of potentially enhancing their growth in vitro as well as their function in vivo.  (+info)

Enumeration of antigen-presenting cells in mice infected with Sendai virus. (8/4245)

Substantial progress has been made in understanding Ag presentation to T cells; however, relatively little is known about the location and frequency of cells presenting viral Ags during a viral infection. Here, we took advantage of a highly sensitive system using lacZ-inducible T cell hybridomas to enumerate APCs during the course of respiratory Sendai virus infection in mice. Using lacZ-inducible T cell hybridomas specific for the immunodominant hemagglutinin-neuraminidase HN421-436/I-Ab and nucleoprotein NP324-332/Kb epitopes, we detected APCs in draining mediastinal lymph nodes (MLNs), in cervical lymph nodes, and also in the spleen. HN421-436/I-Ab- and NP324-332/Kb-presenting cells were readily detectable between days 3 and 9 postinfection, with more APCs present in the MLN than in the cervical lymph nodes. Interestingly, no infectious virus was detected in lymphoid tissue beyond day 6, suggesting that a depot of noninfectious viral Ag survives, in some form, for 2-3 days after viral clearance. Fractionation of the MLN demonstrated that APC frequency was enriched in dendritic cells and macrophages but depleted in the B cell population, suggesting that B cells do not form a large population of APCs during the primary response to this virus.  (+info)

HIV-epitope-specific T cell responses are critical components of the natural immune response to HIV infection, but these cells often become dysfunctional in chronic infection. Structural diversity within the epitope-specific T cell receptor (TCR) repertoire is likely beneficial in the suppression of viral epitope variants. However, the relationship between the structural and clonotypic composition of the HIV-specific TCR repertoire, clonotypic surface and functional phenotype, and avidity for and exposure to antigen is poorly defined. Dominant and sub-dominant epitope-specific T cell clonotypes were identified and the TCR repertoire was assessed over time. Surface expression of PD-1, CD38, CD127, CD45RO, and CCR7 was measured and used to define exhaustion and memory profiles on epitope-specific T cell populations during chronic infection and after initiation of antiretroviral therapy. Dominant clonotypes in chronic infection express a surface phenotype consistent with exhaustion which is ...
BACKGROUNDː The most cause of cancer-related death in women population is breast cancer (BC). Hence, efforts to develop an effective treatment against BC are needed. Since the large proportion of BC is due to over-expression of tumor-associated antigens (TAAs), multi-epitope cancer vaccines are considered as a promising therapeutic approach. The aim of the current study is the production of the novel multi-epitope peptide vaccine against BC in a prokaryotic host. Our novel multi-epitope BC vaccine consists of four sections: 1) cytotoxic T lymphocytes epitopes from human epidermal growth factor receptor, heparanase, mucin 1 protein; 2) helper T lymphocytes epitopes from survivin; 3) a segment of Por B protein a immunostimulatory adjuvant, which was selected from Neisseria meningitides by bioinformatics analysis; 4) a segment of murine ULBP-like transcript 1, which binds to a natural killer group 2 member D receptor in tumor microenvironment with high affinity and stimulates innate immune ...
CD8+ T cells have the potential to control HSV-2 infection. However, limited information has been available on CD8+ T cell epitopes or the functionality of antigen specific T cells during infection or following immunization with experimental vaccines. Peptide panels from HSV-2 proteins ICP27, VP22 and VP13/14 were selected from in silico predictions of binding to human HLA-A*0201 and mouse H-2Kd, Ld and Dd molecules. Nine previously uncharacterized CD8+ T cell epitopes were identified from HSV-2 infected BALB/c mice. HSV-2 specific peptide sequences stabilized HLA-A*02 surface expression with intermediate or high affinity binding. Peptide specific CD8+ human T cell lines from peripheral blood lymphocytes were generated from a HLA-A*02+ donor. High frequencies of peptide specific CD8+ T cell responses were elicited in mice by DNA vaccination with ICP27, VP22 and VP13/14, as demonstrated by CD107a mobilization. Vaccine driven T cell responses displayed a more focused immune response than those induced
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
Advances in the field of T cell immunology have contributed to the understanding that cross-reactivity is an intrinsic characteristic of the T cell receptor (TCR), and that each TCR can potentially interact with many different T cell epitopes. To better define the potential for TCR cross-reactivity between epitopes derived from the human genome, the human microbiome, and human pathogens, we developed a new immunoinformatics tool, JanusMatrix, that represents an extension of the validated T cell epitope mapping tool, EpiMatrix. Initial explorations, summarized in this synopsis, have uncovered what appear to be important differences in the TCR cross-reactivity of selected regulatory and effector T cell epitopes with other epitopes in the human genome, human microbiome, and selected human pathogens. In addition to exploring the T cell epitope relationships between human self, commensal and pathogen, JanusMatrix may also be useful to explore some aspects of heterologous immunity and to examine T cell
Jørgensen KW, Rasmussen M, Buus S, Nielsen M. NetMHCstab - predicting stability of peptide:MHC-I complexes; impacts for CTL epitope...
Jørgensen KW, Rasmussen M, Buus S, Nielsen M. NetMHCstab - predicting stability of peptide:MHC-I complexes; impacts for CTL epitope...
The specificity of T cells is determined by the TCR. T cell populations activated and expanded during most in vivo antigen challenges are highly complex, with diverse TCR repertoires, complicating the detection of these cells. The ideal reagent to identify complex epitope-specific T cell populations would be the natural ligand of the TCR, the MHC/epitope complex. However, the affinity of the TCR-MHC/epitope interaction is very low; the association is characterized by a particularly high dissociation rate. To increase the overall avidity of this interaction, MHC/epitope complexes are multimerized into e.g. tetramers. MHC tetramer reagents conjugated with a fluorescent dye can be used in flow cytometry, allowing highly specific detection and isolation of (complex) epitope specific T cell populations directly ex vivo.. The generation of MHC class I multimer reagents has become well established over the past few years. Beta-2-microglobulin and the heavy chains (HC) of MHC molecules are expressed as ...
TCR affinity associated with functional differences between dominant and subdominant SIV epitope-specific CD8+ T cells in Mamu-A*01+ rhesus monkeys.
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
Strategy for Identifying Dendritic Cell-Processed CD4 T Cell Epitopes from the HIV Gag p24 Protein. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
ECIA™ Intracellular cytokine staining assay is utilized to detect the antigen-specific T cell responses, immunogenic analysis, epitope discovery at a single cell level for both clinical studies and scientific researches.
We have recently described the first true genome-wide screen for CD4+ T cell reactivity directed against MTB in latent TB infected individuals. The approach relied on predictions of HLA binding capacity for a panel of DR, DP and DQ alleles representative of those most commonly expressed in the general population, coupled with high throughput ELISPOT assays. The results identified hundreds of novel epitopes and antigens, and documented the novel observation that T cells in latent MTB infection are confined to the CXCR3+CCR6+ phenotype and largely directed against three antigenic
EpiToolKit provides a collection of methods from computational immunology for the development of novel epitope-based vaccines including HLA ligand or potential T-Cell epitope prediction, an epitope selection framework for vaccine design, and a method to design optimal string-of-beads vaccines. Additionally, EpiToolKit provides several other tools ranging from HLA typing based on NGS data, to prediction of polymorphic peptides ...
MHC Tetramers for detecting specific T-cell populations. Easy immuophenotyping of your t-cell sample! Class I and II antigen specific tetramers available.
Multiple lines of evidence support a role for CD8(+) T cells in control of acute/early HIV replication; however, features of the primary HIV-specific CD8(+) T cell response that may impact on the efficiency of containment of early viral replication remain poorly defined. In this study, we performed a novel, comprehensive analysis of the kinetics of expansion of components of the HIV-specific CD8(+) T cell response in 21 acutely infected individuals. Epitope-specific T cell responses expanded asynchronously during primary infection in all subjects. The most rapidly expanded responses peaked as early as 5 days following symptomatic presentation and were typically of very limited epitope breadth. Responses of additional specificities expanded and contracted in subsequent waves, resulting in successive shifts in the epitope immunodominance hierarchy over time. Sequence variation and escape were temporally associated with the decline in magnitude of only a subset of T cell responses, suggesting that other
Cytotoxic T lymphocytes (CTLs) play a key role in the control of persistent viral infections. Differences in the quality of this cellular immune response influence the long-term outcome of such infections, but the factors that determine which virus-derived peptide epitopes are targeted by CTLs remain poorly understood. Here, we examine the antigen-processing requirements of three human leukocyte antigen (HLA) A*0201-restricted HIV-1 CTL epitopes. Each of these three peptides appears to be generated by a distinct proteolytic pathway, despite presentation on the cell surface in association with the same HLA class I molecule. Presentation of the commonly immunodominant SLYNTVATL (HIV-1 p17 Gag; residues 77-85) epitope was unaffected by inhibition of the proteasome with lactacystin, but was dependent on the presence of the beta-subunit LMP7. These findings are consistent with emerging data on the complexity of peptide epitope generation, and suggest that differences in antigen processing might contribute to
The chimeric antibodies anti-CD20 rituximab (Rtx) and anti-TNFa infliximab (Ifx) induce antidrug antibodies (ADAs) in many patients with inflammatory diseases. Because of the key role of CD4 T lymphocytes in the initiation of antibody responses, we localized the CD4 T cell epitopes of Rtx and Ifx. With the perspective to anticipate immunogenicity of therapeutic antibodies, identification of the CD4 T cell epitopes was performed using cells collected in healthy donors. Nine T cell epitopes were identified in the variable chains of both antibodies by deriving CD4 T cell lines raised against either Rtx or Ifx. The T cell epitopes often exhibited a good affinity for human leukocyte antigen (HLA)-DR molecules and were part of the peptides identified by MHC-associated peptide proteomics assay from HLA-DR molecules of dendritic cells (DCs) loaded with the antibodies. Two-third of the T cell epitopes identified from the healthy donors stimulated peripheral blood mononuclear cells from patients having ...
The ability of HIV-1-specific CD8(+) T cell responses to recognize epitope variants resulting from viral sequence variation in vivo may affect the ease with which HIV-1 can escape T cell control and impact on the rate of disease progression in HIV-1-infected humans. Here, we studied the functional cross-reactivity of CD8 responses to HIV-1 epitopes restricted by HLA class I alleles associated with differential prognosis of infection. We show that the epitope-specific responses exhibiting the most efficient cross-recognition of amino acid-substituted variants were those strongly associated with delayed progression to disease. Not all epitopes restricted by the same HLA class I allele showed similar variant cross-recognition efficiency, consistent with the hypothesis that the reported associations between particular HLA class I alleles and rate of disease progression may be due to the quality of responses to certain critical epitopes. Irrespective of their efficiency of functional cross-recognition, CD8
The present study focused on three Gag CTL epitopes restricted by three common HLA alleles in Japanese people (24). The Gag protein is most commonly targeted by CTL-inducing HIV/AIDS vaccines (15). In our endogenous expression system, three A*0201-restricted epitope variants and one B*5101-restricted epitope variant escaped from the wild-type CTL recognition, and four A24-restricted epitope variants escaped from the A24-restricted 3R mutant-reactive CTL recognition. Intriguingly, two A*0201-restricted variants and three A24-restricted variants escaped from CTL killing when the gag clones were expressed endogenously in the target cells by the HIV-1 vector, despite the fact that the synthetic variant peptides were well recognized by the CTLs when loaded onto the MHC class I molecule exogenously. The peptide titration experiments have revealed that the strength of these variant peptides recognition was almost equivalent to that of the A*0201-restricted wild-type peptide or the A24-restricted 3R ...
The interaction between a CD4+ TH cell and an antigen presenting cell (APC) is a finely tuned event in adaptive immunity. The affinity is dictated by the T cell receptor (TCR) and the characteristics of antigenic peptide epitopes presented on the major histocompatibility complex class II (pMHC class II) molecules on APCs. Due to the high degree of polymorphism in MHC molecules and the vast repertoire of epitopes that may be presented on each, it is an immense challenge to predict epitopes relevant to disease and homeostasis. It would therefore be of very high value to develop a technology that allows for precise experimental identification of T cell epitopes in the absence of any prior knowledge of the relevant antigen, as long as one has access to T cells. We have developed a novel and generic combinatorial display system for MHC class II, based on phage display. Using phage display, one can probe an epitope sequence space orders of magnitude larger than any cellular or chemical system. As a ...
TY - JOUR. T1 - Refinement in the production and purification of recombinant HCMV IE1-pp65 protein for the generation of epitope-specific T cell immunity. AU - Nguyen, Thi Hoang Oanh. AU - Mifsud, Nicole Andrea. AU - Stewart, Lisbeth A. AU - Rose, Mingus J. AU - Etto, Tamara L. AU - Williamson, Nicholas A. AU - Purcell, Anthony Wayne. AU - Kotsimbos, Tom C. AU - Schwarer, Anthony. PY - 2008. Y1 - 2008. UR - http://www.elsevier.com/locate/yprep. M3 - Article. VL - 61. SP - 22. EP - 30. JO - Protein Expression and Purification. JF - Protein Expression and Purification. SN - 1046-5928. IS - 1. ER - ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
Treatment of invasive adenovirus (Ad) disease in hematopoietic stem cell transplant (SCT) recipients with capsid protein hexon-specific donor T cells is under investigation. We propose that cytotoxic T cells (CTLs) targeted to the late protein hexon may be inefficient in vivo because the early Ad protein E3-19K downregulates HLA class I antigens in infected cells. In this study, CD8+ T cells targeted to highly conserved HLA A2-restricted epitopes from the early regulatory protein DNA polymerase (P-977) and late protein hexon (H-892) were compared in peripheral blood (PB) and tonsils of naturally infected adults. In tonsils, epitope-specific pentamers detected a significantly higher frequency of P-977+CD8+ T cells compared to H-892+CD8+ T cells; this trend was reversed in PB. Tonsil epitope-specific CD8+ T cells expressed IFN-γ and IL-2 but not perforin or TNF-α, whereas PB T cells were positive for IFN-γ, TNF-α, and perforin. Tonsil epitope-specific T cells expressed lymphoid homing marker CCR7 and
Immune monitoring of T cell responses increasingly relies on the use of peptide pools. Peptides, when restricted by the same HLA allele, and presented from within the same peptide pool, can compete for HLA binding sites. What impact such competition has on functional T cell stimulation, however, is not clear. Using a model peptide pool that is comprised of 32 well-defined viral epitopes from Cytomegalovirus, Epstein-Barr virus, and Influenza viruses (CEF peptide pool), we assessed peptide competition in PBMC from 42 human subjects. The magnitude of the peptide pool-elicited CD8 T cell responses was a mean 79% and a median 77% of the sum of the CD8 T cell responses elicited by the individual peptides. Therefore, while the effect of peptide competition was evident, it was of a relatively minor magnitude. By studying the dose-response curves for individual CEF peptides, we show that several of these peptides are present in the CEF-pool at concentrations that are orders of magnitude in excess of what is
Despite progress toward understanding the correlates of protective T cell immunity in HIV infection, the optimal approach to Ag delivery by vaccination remains uncertain. We characterized two immunodominant CD8 T cell populations generated in response to immunization of BALB/c mice with a replication-deficient adenovirus serotype 5 vector expressing the HIV-derived Gag and Pol proteins at equivalent levels. The Gag-AI9/H-2Kd epitope elicited high-avidity CD8 T cell populations with architecturally diverse clonotypic repertoires that displayed potent lytic activity in vivo. In contrast, the Pol-LI9/H-2Dd epitope elicited motif-constrained CD8 T cell repertoires that displayed lower levels of physical avidity and lytic activity despite equivalent measures of overall clonality. Although low-dose vaccination enhanced the functional profiles of both epitope-specific CD8 T cell populations, greater polyfunctionality was apparent within the Pol-LI9/H-2Dd specificity. Higher proportions of central ...
Fingerprint Dive into the research topics of Recognition of naturally processed and ovarian cancer reactive CD8 ,sup,+,/sup, T cell epitopes within a promiscuous HLA class II T-helper region of NY-ESO-1. Together they form a unique fingerprint. ...
easYmer HLA-B*46:01 MHC Tetramers Kit can be used to generate monomers with your choice of peptide and to analyze T-cells by flow cytometry.
A method for updating detecting and loading CD volume indexes from a multiple-CD set to a cumulative volume table contained in a computer memory. The method employs an volume index file on each intermediate CD of the set along with a dual index file feature on the last CD of the set. The second index file on the last CD is a cumulative file of all the index files contained on all the CDs of the set. The cumulative index file on the last CD is compared to the cumulative volume table to generate a list of missing volumes which have not already been loaded into computer memory. The method permits determining whether a given CD is a single CD or a CD that is one of a multiple-CD set by detecting the presence of a second volume index file on the CD.
p286/I-Ag7 tetramer-positive CD4+ T cells from G286 mice delay diabetes transfer. Tetramer-positive and -negative cells were sorted from G286 lymph node cells w
Moradian N, Ochs HD, Sedikies C, Hamblin MR, Camargo CA Jr, Martinez JA, Biamonte JD, Abdollahi M, Torres PJ, Nieto JJ, Ogino S, Seymour JF, Abraham A, Cauda V, Gupta S, Ramakrishna S, Sellke FW, Sorooshian A, Wallace Hayes A, Martinez-Urbistondo M, Gupta M, Azadbakht L, Esmaillzadeh A, Kelishadi R, Esteghamati A, Emam-Djomeh Z, Majdzadeh R, Palit P, Badali H, Rao I, Saboury AA, Jagan Mohan Rao L, Ahmadieh H, Montazeri A, Fadini GP, Pauly D, Thomas S, Moosavi-Movahed AA, Aghamohammadi A, Behmanesh M, Rahimi-Movaghar V, Ghavami S, Mehran R, Uddin LQ, Von Herrath M, Mobasher B, Rezaei N. ...
Dear Netters, Does anyone know of any software for the prediction of T-cell epitopes from peptide sequences? I have access to Mac, PC, VAX, SG & UNIX so the program format does not matter. Hope to hear from someone soon! Thanks, Brian Robertson Max-Planck-Institut fuer Biologie Abt. Infektionsbiologie D74 Tuebingen, FRG. Email robertson at mpib-tuebingen.mpg.de ...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
CTL recognition and antagonism by naturally occurring p17 variants. Recognition of variant peptides by two donor 008 clones (18 and 20) (a and b) at an ET of 8
This study demonstrates that use of structural information improves the definition and optimization of cytotoxic T lymphocyte (CTL) epitopes. Epitope optimization usually requires numerous truncated peptides or a reverse immunogenetic approach, where the peptide binding motif is used to predict epitopes. These binding motifs do not reliably predict all peptides which are CTL epitopes. Comparison of 24 peptides eluted from HLA-B8 with 10 HLA-B8-restricted defined CTL epitopes demonstrated that known epitopes varied considerably at anchor positions. We used structural information based on determination of the crystal structure of the HLA-B8-GGKKKYKL complex to reassess previously described CTL epitopes, to predict new epitopes, and to predict the consequences of naturally occurring variation within epitopes. These predictions were confirmed by cytotoxicity and binding assays. Use of combined structural and immunological data more accurately defines the true peptide-binding motif of a restriction element
Virus-specific cytotoxic T-lymphocyte (CTL) responses are critical in the control of human immunodeficiency virus type 1 (HIV-1) infection and will play an important part in therapeutic and prophylactic HIV-1 vaccines. The identification of virus-specific epitopes that are efficiently recognized by CTL is the first step in the development of future vaccines. Here we describe the immunological characterization of a number of novel HIV-1-specific, HLA-A2-restricted CTL epitopes that share a high degree of conservation within HIV-1 and a strong binding to different alleles of the HLA-A2 superfamily. These novel epitopes include the first reported CTL epitope in the Vpr protein. Two of the novel epitopes were immunodominant among the HLA-A2-restricted CTL responses of individuals with acute and chronic HIV-1 infection. The novel CTL epitopes identified here should be included in future vaccines designed to induce HIV-1-specific CTL responses restricted by the HLA-A2 superfamily and will be important to
PubMed journal article Identification and characterization of a human agonist cytotoxic T-lymphocyte epitope of human prostate-specific antige were found in PRIME PubMed. Download Prime PubMed App to iPhone, iPad, or Android
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
In the course of constructing a recombinant vaccinia virus encoding the influenza A nucleoprotein (NP) gene preceded by the hemagglutinin leader sequence, we isolated a single base-pair deletion mutant which gave rise to L+NP(1-159) in which only the first 159 amino acids were in frame. Despite this, when we infected target cells, we found that the point mutant was able to sensitize them for lysis not only by cytotoxic T cells recognizing residues 50-58 (the in-frame portion), but also by CTL to epitopes which are downstream of the mutation (366-374 and 378-386). Furthermore, normal C57BL/6 mice can be primed with the frameshift NP to recognize the immunodominant Db-restricted epitope 366-374 (which is out of frame). Experiments in which the mutant gene product was processed in the endoplasmic reticulum of target cells suggested that the apparent suppression occurred during polypeptide extension.
In the course of constructing a recombinant vaccinia virus encoding the influenza A nucleoprotein (NP) gene preceded by the hemagglutinin leader sequence, we isolated a single base-pair deletion mutant which gave rise to L+NP(1-159) in which only the first 159 amino acids were in frame. Despite this, when we infected target cells, we found that the point mutant was able to sensitize them for lysis not only by cytotoxic T cells recognizing residues 50-58 (the in-frame portion), but also by CTL to epitopes which are downstream of the mutation (366-374 and 378-386). Furthermore, normal C57BL/6 mice can be primed with the frameshift NP to recognize the immunodominant Db-restricted epitope 366-374 (which is out of frame). Experiments in which the mutant gene product was processed in the endoplasmic reticulum of target cells suggested that the apparent suppression occurred during polypeptide extension.
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
The proteasome, an essential component of the ATP-dependent proteolytic pathway in eukaryotic cells, is responsible for the degradation of most cellular proteins and is believed to be the main source of MHC class I-restricted antigenic peptides for presentation to CTL. Inhibition of the proteasome by lactacystin or various peptide aldehydes can result in defective Ag presentation, and the pivotal role of the proteasome in Ag processing has become generally accepted. However, recent reports have challenged this observation. Here we examine the processing requirements of two HLA A*0201-restricted epitopes from HIV-1 reverse transcriptase and find that they are produced by different degradation pathways. Presentation of the C-terminal ILKEPVHGV epitope is impaired in ME275 melanoma cells by treatment with lactacystin, and is independent of expression of the IFN-gamma-inducible proteasome beta subunits LMP2 and LMP7. In contrast, both lactacystin treatment and expression of LMP7 induce the presentation of
The identification of class II binding peptide epitopes from autoimmune disease-related antigens is an essential step in the development of antigen-specific immune modulation therapy. In the case of type 1 diabetes, T cell and B cell reactivity to the autoantigen glutamic acid decarboxylase 65 (GAD65) is associated with disease development in humans and in nonobese diabetic (NOD) mice. In this study, we identify two DRB1*0401-restricted T cell epitopes from human GAD65, 274-286, and 115-127. Both peptides are immunogenic in transgenic mice expressing functional DRB1*0401 MHC class II molecules but not in nontransgenic littermates. Processing of GAD65 by antigen presenting cells (APC) resulted in the formation of DRB1*0401 complexes loaded with either the 274-286 or 115-127 epitopes, suggesting that these naturally derived epitopes may be displayed on APC recruited into pancreatic islets. The presentation of these two T cell epitopes in the islets of DRB1*0401 individuals who are at risk for type 1
Vaccines designed to stimulate CTL should be able to deliver protein antigens to the cytoplasm of host cells for processing and presentation by MHC-I. Many systems have been exploited to accomplish cytoplasmic delivery, including viral and bacterial vectors and DNA vaccines (7, 8, 14, 22, 27, 33, 36). There have also been reports demonstrating that antiviral immunity can be primed in mice vaccinated with a recombinant Bordetella adenylate cyclase toxin incorporating a CTL epitope from LCMV (11, 29, 31); however, the data demonstrated protection only when the recombinant toxin was injected in the presence of an adjuvant, aluminum hydroxide (29). Therefore, it remains unclear whether the antiviral protection was a result of toxin delivery or adjuvant activity. This report describes the use of a nontoxic, truncated form of anthrax toxin as an epitope delivery system without the use of adjuvant.. We demonstrate that protective immunity against a viral pathogen, LCMV, is generated in BALB/c mice ...
MHC-binding predictions have facilitated T cell epitope discovery by narrowing the search space to a manageable number of likely peptide candidates. Compared with approaches that do not use predictions, such as screening overlapping peptides, a downside of the prediction approach was that peptides of noncanonical lengths were missed (35). Naively, one could simply extend binding predictions to peptides of any length and rank all peptides based on their predicted affinity. But, as demonstrated in our study, although peptides of noncanonical lengths might have similar or even better predicted binding affinities than the canonical 9mer peptides, they end up being underrepresented among the naturally presented ligands eluted from MHC molecules and, consequently, are recognized less frequently by T cells. In this study, we explained these similarities between the length profiles of naturally presented peptides by fitting a common, underlying peptide-length distribution. This common length ...
The precise role played by HIV-specific cytotoxic T lymphocytes (CTL) in HIV infection remains controversial. Despite strong CTL responses being generated during the asymptomatic phase, the virus persists and AIDS ultimately develops. It has been argued that the virus is so variable, and the virus turnover so great that escape from CTL recognition would occur continually, but so far there is limited evidence for CTL escape. The opposing argument is that evidence for CTL escape is present but hard to find because multiple anti-HIV immune responses are acting simultaneously during the asymptomatic phase of infection. We describe six donors who make a strong CTL response to an immunodominant HLA-B27-restricted epitope. In the two donors who progressed to AIDS, CTL escape to fixation by the same mutation was observed, but only after 9-12 years of epitope stability. CTL escape may play an important role in the pathogenesis of HIV infection.
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
In this study we show that the HPV16 E7-specific CD8+ T cell response is far more vigorous after vaccination with a HPV16 E7-derived 35-residue long peptide than following vaccination with the minimal CTL epitope. Our data suggest that two distinct DC-related mechanisms lie at the basis of this result. First, the long peptide contains both a CTL epitope and a Th epitope. Prime-boost vaccinations of wild-type, MHC class II−/−, and CD40−/− mice show that the CD40-CD40L interactions between APC and E7-specific Th cells contribute considerably to the level of the CD8+ T cell response. Second, under circumstances excluding T cell help, a comparison of the CD8+ T cell responses induced by either the minimal CTL epitope (nine residues) or the long peptide combined with DC-activating agents demonstrates that vaccination with the long peptide results in far better responses. This suggests that compared with the short minimal CTL epitope the long peptide is much better presented by professional ...
A long-standing question in the field of immunology concerns the factors that contribute to Th cell epitope immunodominance. For a number of viral membrane proteins, Th cell epitopes are localized to exposed protein surfaces, often overlapping with Ab binding sites. It has therefore been proposed that Abs on B cell surfaces selectively bind and protect exposed protein fragments during Ag processing, and that this interaction helps to shape the Th cell repertoire. While attractive in concept, this hypothesis has not been thoroughly tested. To test this hypothesis, we have compared Th cell peptide immunodominance in normal C57BL/6 mice with that in C57BL/6( micro MT/ micro MT) mice (lacking normal B cell activity). Animals were first vaccinated with DNA constructs expressing one of three different HIV envelope proteins, after which the CD4(+) T cell response profiles were characterized toward overlapping peptides using an IFN-gamma ELISPOT assay. We found a striking similarity between the peptide ...
We recently discover a new immune escape mechanism that may help viruses escape from immune detection, which might compromise vaccine efficacy. Viruses that cause chronic infection in human contain higher numbers of T cell epitopes whose TCR-facing amino acids are identical to those of numerous peptides from the human proteome. We postulate that viruses that incorporate such human-like epitopes may exploit host tolerance to avoid or suppress effector responses. In order to predict these human-like epitopes, we developed an immunoinformatics tool, JanusMatrix.. Using JanusMatrix, we have identified T cell epitopes in H7N9 influenza HA protein that are highly conserved with human genome epitopes, and these epitopes possess low immunogenicity, activate natural Tregs and suppress bystander effector T cell responses in vitro. The human like T cell epitopes may contribute to the delayed, low titer of H7N9 hemagglutination inhibiting antibody responses and diminished seroconversion rates that have been ...
This graph shows the total number of publications written about Immunodominant Epitopes by people in this website by year, and whether Immunodominant Epitopes was a major or minor topic of these publications ...
The Experimental Analysis of Mutlitple Computationally-Driven Methods for the Deletion of Broadly Distributed T cell Epitopes in a Functional Biotherapeutic Candidate A Thesis Submitted to the Faculty in partial fulfillm ent o f the requirements for the degree of D octor o f Philosophy by Regina Salvat Thayer School o f Engineering D artmouth College Hanover, N ew Hampshire January 4, 2015 Chairman Exam ining Committee: Professor Karl Griswold M em ber Professor M argaret Ackerman M em ber Professor Chris Bailey-Kellogg M emb er___________________________ Professor Lenny M oise F. Jon Kull Dean o f Graduate Studies ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
Fingerprint Dive into the research topics of TAP-independent presentation of CTL epitopes by Trojan antigens. Together they form a unique fingerprint. ...
Peptide tools for target & epitope discovery, development of treatment & diagnosis of allergies, such as food allergy, atopic dermatitis, allergic asthma…
Rational design of immunotherapeutics relies on clear knowledge of the immunodominant epitopes of antigens. Current methods for identifying kinetically stable peptide-MHC complexes are in many cases i
Peptides that combine poorly to MHC course We substances elicit low functional avidity Capital t cell reactions often. immune system reactions can become increased towards Capital t cell epitopes with low practical avidity by raising antigen denseness. We also determined a heteroclitic epitope (RCVIFANI) that elicited a Capital t cell response with almost full cross-reactivity with indigenous epitope and proven improved MHC-peptide plethora likened to indigenous S i9000598. Structural and thermal dissolve studies indicated that the Queen600V replacement improved balance of the peptide-MHC complicated without significantly changing the antigenic surface area, causing in cross-reactive Big t cell reactions extremely. Our data high light that improved pMHC Rabbit polyclonal to IL25 complicated screen contributes to heteroclitic epitope effectiveness and explain guidelines for increasing immune system reactions that cross-react with the indigenous epitope. Intro growth and Virus distance both ...
T cells play pivotal roles in shaping host immune responses in infectious diseases, autoimmunity and cancer. The activation of T cells requires immune and growth factor-derived signals. However, alterations in nutrients and metabolic signals tune T cell responses by impinging upon T cell fates and immune functions. In this review, we summarize how key nutrients, including glucose, amino acids and lipids, and their sensors and transporters shape T cell responses. We also briefly discuss regulation of T cell responses by oxygen and energy sensing mechanisms.
Analysis of cytotoxic T cell epitopes in relation to cancer. / Stranzl, Thomas; Brunak, Søren (Main supervisor); Larsen, Mette Voldby (Supervisor).. Kgs. Lyngby : Technical University of Denmark (DTU), 2012. 97 p.. Publication: Research › Ph.D. thesis - Annual report year: 2012 ...
In this study we characterized the CTL response induced in vivo against TS/A mouse adenocarcinoma cells genetically engineered to express different cytokine or costimulatory molecules, i.e., IFN-α, IFN-γ, IL-4, and B7.1. Independent of the molecule introduced, the CTL response elicited was constantly directed against a single immunodominant epitope represented by the AH1 peptide derived from the gp70 product of an endogenous MuLV and corresponding to amino acids 423-431 of the protein (19) . Indeed, tumor rejection was accompanied by increases in a CD8+ T-cell population that was stainable with gp70-specific tetramers; moreover, independent in vitro restimulation of splenocytes from mice that had rejected a primary TS/A tumor challenge with either gp70423-431 peptide or engineered TS/A, which provided the entire antigenic array of the tumor cells, produced a similar large increase in CTLs specifically recognizing the AH1 antigenic epitope. Finally, MLTC lytic activity could be inhibited in an ...
Authors: AI Webb, MA Dunstone, WS Chen, MI Aguilar, QY Chen, H Jackson, L Chang, L Kjer-Nielsen, T Beddoe, J McCluskey, J Rossjohn, AW Purcell
"Mapping and characterization of visna/maedi virus cytotoxic T-lymphocyte epitopes". J. Gen. Virol. 89 (Pt 10): 2586-96. doi: ... This causal lentivirus can be found in monocytes, lymphocytes and macrophages of infected sheep in the presence of humoral and ... Infected differentiated monocytes, also known as macrophages, will continuously present VMV antigens inducing T-lymphocytes to ... visna virus does not infect T-lymphocytes. The relationship of visna and HIV as lentiviruses was first published in 1985 by ...
In RHD, molecular mimicry results in incorrect T cell activation, and these T lymphocytes can go on to activate B cells, which ... due to the cross-reactivity of antibodies generated as a result of epitope sharing. The valvular endothelium is a prominent ... The dominant contributors are a component of MHC class II molecules, found on lymphocytes and antigen-presenting cells, ... Rheumatic valves display increased expression of VCAM-1, a protein that mediates the adhesion of lymphocytes. Self-antigen- ...
The TCRs of T lymphocytes recognise only sequential epitopes, also called linear epitopes, of only peptides and only if coupled ... It presents epitopes to killer T cells, also called cytotoxic T lymphocytes (CTLs). A CTL expresses CD8 receptors, in addition ... Classical MHC molecules present epitopes to the TCRs of CD8+ T lymphocytes. Nonclassical molecules (MHC class IB) exhibit ... T lymphocytes can detect a peptide displayed at 0.1%-1% of the MHC molecules. In their development in the thymus, T lymphocytes ...
2006). "A molecular approach to the identification of cytotoxic T-lymphocyte epitopes with Equine Herpes-1". Journal of General ...
"The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides". ... Bowness, Paul (1993). Recognition of antigen and superantigen by cytotoxic lymphocytes (Thesis). Thesis DPhil--University of ... "Vertical T cell immunodominance and epitope entropy determine HIV-1 escape". The Journal of Clinical Investigation. 123 (1): ... "Phenotypic analysis of antigen-specific T lymphocytes". Science. 274 (5284): 94-96. Bibcode:1996Sci...274...94A. doi:10.1126/ ...
"IFN-gamma exposes a cryptic cytotoxic T lymphocyte epitope in HIV-1 reverse transcriptase". Journal of Immunology. 162 (12): ... Madani N, Kabat D (Dec 1998). "An endogenous inhibitor of human immunodeficiency virus in human lymphocytes is overcome by the ... is the processing of numerous MHC class-I restricted T cell epitopes. This gene encodes a member of the proteasome B-type ... is the major degradation machinery that degrades the antigen and present the resulting peptides to cytotoxic T lymphocytes. The ...
Perlmann's further research included cytotoxicity of human lymphocytes and immunogen selection and epitope mapping for malaria ... immunogen selection and epitope mapping [Review]. Vaccine 1998;6:183-187 Voller A, Huldt G, Thors C, Engvall E. New serological ... occurrence of immunoglobulin-dependent and immunoglobulin-independent mechanisms in natural cytotoxicity of human lymphocytes. ...
3 HLA-A2 restricted epitopes in CTAG1B were identified as the recognition sites for CD8+ cytotoxic T lymphocytes. Integrated ... A2-binding peptide epitopes". The Journal of Experimental Medicine. 187 (2): 265-70. doi:10.1084/jem.187.2.265. PMC 2212106. ... "NY-ESO-1 119-143 is a promiscuous major histocompatibility complex class II T-helper epitope recognized by Th1- and Th2-type ... "Efficient simultaneous presentation of NY-ESO-1/LAGE-1 primary and nonprimary open reading frame-derived CTL epitopes in ...
... containing a mutated epitope recognized by autologous tumor-infiltrating T lymphocytes". J. Immunol. 166 (4): 2871-7. doi: ...
... encoding the interleukin 1 receptor antagonist and six epitopes recognized by human cytolytic T lymphocytes". Cancer Res. 60 ( ...
... cytotoxic T-lymphocyte responses against dominant and recessive epitopes of influenza virus nucleoprotein induced by DNA ... CTL responses can be raised against immunodominant and immunorecessive CTL epitopes, as well as subdominant CTL epitopes, in a ... One of the advantages of DNA vaccines is that they are able to induce cytotoxic T lymphocytes (CTL) without the inherent risk ... This may prove to be a useful tool in assessing CTL epitopes and their role in providing immunity. Cytotoxic T-cells recognise ...
"Amphipathic segment of the nicotinic receptor alpha subunit contains epitopes recognized by T lymphocytes in myasthenia gravis ...
The parts of an antigen that interact with an antibody molecule or a lymphocyte receptor, are called epitopes, or antigenic ... T and B lymphocytes are the cells of the adaptive immune system. The human body has about 2 trillion lymphocytes, which are 20- ... In jawless fishes, two subsets of lymphocytes use variable lymphocyte receptors (VLRs) for antigen binding. Diversity is ... Lymphocyte receptors, Ig and TCR, are found in all jawed vertebrates. The most ancient Ig class, IgM, is membrane-bound and ...
Lymphocyte anergy pathways involve induction of some, but not all of the signaling pathways used during lymphocyte activation. ... demonstrated that epitope-mapping tools could accurately identify the epitopes responsible for 95% of the murine T-cell ... It may have been expected that B lymphocytes and T lymphocytes would cluster separately from each other, or that natural killer ... T-cell and B-cell epitope mapping algorithms can computationally predict epitopes based on the genomic sequence of pathogens, ...
The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer ... "A gene encoding antigenic peptides of human squamous cell carcinoma recognized by cytotoxic T lymphocytes". J Exp Med. 187 (3 ... "Identification of a SART-1-derived peptide capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes". ... required for induction of histocompatibility leukocyte antigen-class I-restricted and tumor-specific cytotoxic T lymphocytes by ...
"The antigen-specific induction of normal human lymphocytes in vitro is down-regulated by a conserved HIV p24 epitope". Immunol ... Sewell WA, Brown MH, Dunne J, Owen MJ, Crumpton MJ (1986). "Molecular cloning of the human T-lymphocyte surface CD2 (T11) ... It interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in ... Schraven B, Samstag Y, Altevogt P, Meuer SC (1990). "Association of CD2 and CD45 on human T lymphocytes". Nature. 345 (6270): ...
... the differentiated DCs present the allergenic epitope associated with the allergen to T lymphocytes. These T cells then divide ... In a similar fashion, cytotoxic T lymphocytes patrol an area of skin and play an important role in controlling both the ... Their immunology centres on the interaction of immunoregulatory cytokines and discrete subpopulations of T lymphocytes. The ... and present the antigen to T-lymphocytes. This process is controlled by cytokines and chemokines - with tumor necrosis factor ...
"The antigen-specific induction of normal human lymphocytes in vitro is down-regulated by a conserved HIV p24 epitope". ... Implications for the relationship of NK and T lymphocytes". Journal of Immunology. 149 (6): 1876-80. PMID 1387664. Wong S, ... "A synthetic peptide with sequence identity to the transmembrane protein GP41 of HIV-1 inhibits distinct lymphocyte activation ... "Modulation of CD4 lateral interaction with lymphocyte surface molecules induced by HIV-1 gp120". European Journal of Immunology ...
Vaccination with cytotoxic T lymphocyte epitope‐containing peptide protects against a tumor induced by human papillomavirus ... In addition, it is likely that protein vaccination leads to presentation of epitopes in the context of various HLA alleles, and ... Cooperation between helper T cells and cytotoxic T lymphocyte precursors. Annals of the New York Academy of Sciences, 532(1), ... The inclusion of CD4+ epitopes into MAGE-3 vaccination studies has recently been facilitated by the identification of several ...
2006). "Profiling lymphocyte subpopulations in peripheral blood under efalizumab treatment of psoriasis by multi epitope ligand ... The Toponome Imaging Systems (TIS) and multi-epitope-ligand cartographs (MELC) represent different stages of the ICM ... Ademmer; Ebert; Müller-Ostermeyer; Friess; Büchler; Schubert; Malfertheiner (April 1998). "Effector T lymphocyte subsets in ... "Comparative Multi-Epitope-Ligand-Cartography reveals essential immunological alterations in Barrett's metaplasia and esophageal ...
Epitope spreading or epitope drift - when the immune reaction changes from targeting the primary epitope to also targeting ... In this form of the disease, the absence of lymphocytes can accelerate organ damage, and intravenous IgG administration can be ... Epitope modification or Cryptic epitope exposure - this mechanism of autoimmune disease is unique in that it does not result ... Pike B, Boyd A, Nossal G (1982). "Clonal anergy: the universally anergic B lymphocyte". Proceedings of the National Academy of ...
1992). "The antigen-specific induction of normal human lymphocytes in vitro is down-regulated by a conserved HIV p24 epitope". ... 1996). "Composition of TCR-CD3 complex in human intestinal intraepithelial lymphocytes: lack of Fc epsilon RI gamma chain". Int ... 1989). "Dephosphorylation of the human T lymphocyte CD3 antigen". Eur. J. Biochem. 181 (1): 55-65. doi:10.1111/j.1432-1033.1989 ... "A synthetic peptide with sequence identity to the transmembrane protein GP41 of HIV-1 inhibits distinct lymphocyte activation ...
1992). "The antigen-specific induction of normal human lymphocytes in vitro is down-regulated by a conserved HIV p24 epitope". ... 1987). "The human T3 gamma chain is phosphorylated at serine 126 in response to T lymphocyte activation". J. Biol. Chem. 262 ( ... Implications for the relationship of NK and T lymphocytes". J. Immunol. 149 (6): 1876-80. PMID 1387664. Letourneur F, Klausner ... 1989). "Dephosphorylation of the human T lymphocyte CD3 antigen". Eur. J. Biochem. 181 (1): 55-65. doi:10.1111/j.1432-1033.1989 ...
... ic escape Antitoxin Conformational epitope Epitope Linear epitope Magnetic immunoassay Neutralizing antibody Original ... They become activated and start to secrete cytokines, substances that activate cytotoxic T lymphocytes (CTL), antibody- ... Using the "lock and key" metaphor, the antigen can be seen as a string of keys (epitopes) each of which matches a different ... A hapten is a small molecule that changes the structure of an antigenic epitope. In order to induce an immune response, it ...
This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in ... of a gene coding for a protein possessing shared tumor epitopes capable of inducing HLA-A24-restricted cytotoxic T lymphocytes ...
There is a large diversity of epitopes recognized and, as a result, it is possible for some B and T lymphocytes to develop with ... Each of these lymphocytes express specificity to a particular epitope, or the part of an antigen to which B cell and T cell ... this allows microorganisms with epitopes similar to host antigen to escape recognition and detection by T and B lymphocytes. ... B and T lymphocytes are tested for their affinity for self MHC/peptide complexes before leaving the primary lymphoid organs and ...
MECA-79 mAb inhibits lymphocyte emigration through HEVs into lymph nodes in vivo and lymphocyte adhesion to lymph node and ... The MECA-79 carbohydrate epitope decorates a family of HEV counter-receptors for L-selectin, both in mouse and human16. Another ... This illustrates the function of HEVs in lymphocyte recruitment, and explains why these vessels were implicated in lymphocyte ... express specialized ligands for lymphocytes and are able to support high levels of lymphocyte extravasation). HEVs enable naïve ...
Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ... and lymphocyte aggregation". Journal of Immunology. 153 (9): 3847-61. PMID 7523506. as referenced in paragraph 146 of the PCT ... "Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, ... mucosal addressin-1, fibronectin, and lymphocyte aggregation. Andrew DP, Berlin C, Honda S, Yoshino T, Hamann A, Holzmann B, ...
... cytotoxic T lymphocyte epitope in a human glioma-associated antigen, interleukin 13 receptor alpha2 chain". Clinical Cancer ...
This activity is required to generate signal sequence-derived human lymphocyte antigen-E epitopes that are recognized by the ... 2002). "Intramembrane proteolysis of signal peptides: an essential step in the generation of HLA-E epitopes". J. Immunol. 167 ( ...
Common variable immunodeficiency is thought to be due to a problem in the differentiation from lymphocytes to plasma cells. The ... Plasma cells are large lymphocytes with a considerable nucleus-to-cytoplasm ratio and a characteristic appearance on light ...
Binder M, Otto F, Mertelsmann R, Veelken H, Trepel M. (2006). "The epitope recognized by rituximab". Blood. 108 (6): 1975-1978 ... It increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen). ... including non-Hodgkin's lymphoma and lymphocyte predominant subtype, of Hodgkin's Lymphoma.[12] ...
Binder M, Otto F, Mertelsmann R, Veelken H, Trepel M. (2006). "The epitope recognized by rituximab". Blood. 108 (6): 1975-1978 ... It increases MHC II and adhesion molecules LFA-1 and LFA-3 (lymphocyte function-associated antigen). ... "B-Lymphocyte Depletion in Patients With Myalgic Encephalomyelitis/Chronic Fatigue Syndrome: A Randomized, Double-Blind, Placebo ... including non-Hodgkin's lymphoma and lymphocyte predominant subtype, of Hodgkin's Lymphoma.[12] This also includes ...
1992). "The antigen-specific induction of normal human lymphocytes in vitro is down-regulated by a conserved HIV p24 epitope ... 1992). "The lymphocyte-specific tyrosine protein kinase p56lck is endocytosed in Jurkat cells stimulated via CD2.". J. Immunol. ... Schraven B, Samstag Y, Altevogt P, Meuer SC (1990). "Association of CD2 and CD45 on human T lymphocytes.". Nature 345 (6270): ... Sayre PH, Reinherz EL (1989). "Structure and function of the erythrocyte receptor CD2 on human T lymphocytes: a review.". Scand ...
T cells (T lymphocytes) - T lymphocyte proliferation assay - T lymphocytes - T suppressor cells - T4 cell - T4 cells (T-helper ... epitope - Epstein-Barr virus (EBV) - erythema - erythema multiforme - erythrocytes - etiology - exogenous - exotoxin - expanded ... B-cell lymphoma - B cells - B lymphocytes (B cells) - bactericidal - bacteriostatic - bacterium - baculovirus - baseline - ... lymphocyte - lymphoid interstitial pneumonitis (LIP) - lymphoid organs - lymphoid tissue - lymphokine-activated killer cells ( ...
Lymphocyte. Main article: Lymphocyte. Lymphocytes are much more common in the lymphatic system than in blood. Lymphocytes are ... Lymphocyte. 30%. Small lymphocytes 7-8. Large lymphocytes 12-15. *B cells: releases antibodies and assists activation of T ... lymphocytes) by hematopoietic lineage (cellular differentiation lineage).[6] Lymphocytes can be further classified as T cells, ... Lymphocytes include: *B cells make antibodies that can bind to pathogens, block pathogen invasion, activate the complement ...
T independent antigen elicits antibody production by B lymphocytes without T lymphocyte involvement. There are 2 distinct ... Second group of TI antigens consists mainly of highly repetitive surface structures (epitopes) of encapsulated bacteria. They ... Antibody production independent of T lymphocytes[edit]. For most protein antigens, the production of antibodies by B ... But when the concentration of TI-1 is lower, it can activate only B lymphocytes with specific binding of TI-1 on their BCR, and ...
Epitope) கொண்டுள்ளன. இந்த epitope களே பிறபொருளெதிரியாக்கிகளில் காணப்படும், பிறபொருளெதிரிகளால் அடையாளப்படுத்தக் கூடிய ... "Antibody to histo-blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood ... A கிளைக்கோ புரதத்திலுள்ள α-D-N-galactosamine ஐ ஒத்த epitope களையுடைய இன்ஃபுளுவென்சா வைரசுக்கு எதிரான நோய் எதிர்ப்பாற்றல் முறைமை ... அதேபோல் B கிளைக்கோ புரதத்திலுள்ள α-D-galactose ஐ ஒத்த epitope களையுடைய கிராம்-நெகட்டிவ் பாக்டீரீயாக்களான, E.coli ...
For example, Lymphocyte T-Cell Immune Modulator inhibits viral growth in the murine model of influenza.[225] ... "A highly conserved neutralizing epitope on group 2 influenza A viruses". Science. 333 (6044): 843-50. Bibcode:2011Sci...333.. ... Typically, biologics do not target metabolic pathways like anti-viral drugs, but stimulate immune cells such as lymphocytes, ... "Lymphocyte T-Cell Immunomodulator: Review of the ImmunoPharmacology of a new Veterinary Biologic" (PDF). Journal of Applied ...
Chen JB, Wu PC, Hung AF, Chu CY, Tsai TF, Yu HM, Chang HY, Chang TW (February 2010). "Unique epitopes on C epsilon mX in IgE-B ... The B lymphocyte differentiation and maturation pathway that eventually generate IgE-secreting plasma cells go through the ... "Targeting membrane-expressed IgE B cell receptor with an antibody to the M1 prime epitope reduces IgE production". Sci Transl ... "The CD23a and CD23b proximal promoters display different sensitivities to exogenous stimuli in B lymphocytes". Genes Immun. 3 ...
Many cells of the immune system are required for this process, including lymphocytes (T-cells and B-cells) and antigen ... Deshmukh, US; Bagavant, H; Lewis, J; Gaskin, F; Fu, SM (November 2005). "Epitope spreading within lupus-associated ... In normal physiology, lymphocytes that recognise human proteins (autoantigens) either undergo programmed cell death (apoptosis ... This self-tolerance means that lymphocytes should not incite an immune response against human cellular antigens. Sometimes, ...
"Simultaneous epitope and transcriptome measurement in single cells". Nature Methods. 14 (9): 865-868. doi:10.1038/nmeth.4380 ... "High-throughput targeted long-read single cell sequencing reveals the clonal and transcriptional landscape of lymphocytes" ...
Neutrophils are normally found in the bloodstream and are the most abundant type of phagocyte, constituting 60% to 65% of the total circulating white blood cells.[4] One litre of human blood contains about five billion (5x109) neutrophils,[5] which are about 12-15 micrometers in diameter.[6] Once neutrophils have received the appropriate signals, it takes them about thirty minutes to leave the blood and reach the site of an infection.[7] Neutrophils do not return to the blood; they turn into pus cells and die.[7] Mature neutrophils are smaller than monocytes, and have a segmented nucleus with several sections(two to five segments); each section is connected by chromatin filaments. Neutrophils do not normally exit the bone marrow until maturity, but during an infection neutrophil precursors called myelocytes and promyelocytes are released.[8]. Neutrophils have three strategies for directly attacking micro-organisms: phagocytosis (ingestion), release of soluble anti-microbials (including granule ...
Epitope spreading or epitope drift - when the immune reaction changes from targeting the primary epitope to also targeting ... Suppressor population or Regulatory T cell theory, wherein regulatory T-lymphocytes (commonly CD4+FoxP3+ cells, among others) ... Epitope modification or Cryptic epitope exposure - this mechanism of autoimmune disease is unique in that it does not result ... Idiotype Cross-Reaction - Idiotypes are antigenic epitopes found in the antigen-binding portion (Fab) of the immunoglobulin ...
IELs: intraepithelial lymphocytes). *^ a b Lionetti E, Francavilla R, Pavone P, Pavone L, Francavilla T, Pulvirenti A, Giugno R ... Fleckenstein B, Molberg Ø, Qiao SW, Schmid DG, von der Mülbe F, Elgstøen K, Jung G, Sollid LM (2002). "Gliadin T cell epitope ... Therefore, these forms of the receptor are more likely to activate T lymphocytes and initiate the autoimmune process.[28] ... Biopsy of small bowel showing coeliac disease manifested by blunting of villi, crypt hypertrophy, and lymphocyte infiltration ...
The parts of an antigen that interact with an antibody molecule or a lymphocyte receptor, are called epitopes, or antigenic ... LymphocytesEdit. Main article: Lymphocyte. T and B lymphocytes are the cells of the adaptive immune system. The human body has ... In jawless fishes, two subsets of lymphocytes use variable lymphocyte receptors (VLRs) for antigen binding.[33] Diversity is ... CD8+ T lymphocytes and cytotoxicityEdit. Main article: Cytotoxic T cell. Cytotoxic T cells (also known as TC, killer T cell, or ...
To obtain antibody that is specific for a single epitope of an antigen, antibody-secreting lymphocytes are isolated from the ... The B lymphocyte, in this ready-to-respond form, is known as a "naive B lymphocyte." The naive B lymphocyte expresses both ... Each Ig unit (labeled 1) has two epitope binding Fab regions, so IgM is capable of binding up to 10 epitopes. ... The antibody's paratope interacts with the antigen's epitope. An antigen usually contains different epitopes along its surface ...
"Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen". Bio/Technology. 12 ... of the drug were found by guiding the selection of human antibodies from phage display repertoires to a single epitope of an ...
A process called "epitope spreading" or "provoked immunity" may mitigate this weakness, as sometimes an immune response to a ... Shankaran V, Ikeda H, Bruce AT, White JM, Swanson PE, Old LJ, Schreiber RD (2001-04-26). "IFNgamma and lymphocytes prevent ... Defined tumor antigens decrease the risk of autoimmunity, but because the immune response is directed to a single epitope, ...
The epitope XNAs target is an α-linked galactose moiety, Gal-α-1,3Gal (also called the α-Gal epitope), produced by the enzyme α ... Lymphocytes, which were previously activated by antigens in the vessel wall of the graft, activate macrophages to secrete ... Just as the α1,3G epitope is a major problem in xenotransplantation, so too is dysregulated coagulation a cause of concern. ... and is mediated by natural killer cells which accumulate in and damage the xenograft and T-lymphocytes which are activated by ...
Lymphocytes include two classes that enact adaptive immunity, also called specific immunity. Lymphocytes of specific immunity T ... When the paratope of Ig class gamma (IgG) binds its matching epitope, IgG's Fc region conformationally shifts and can host a ... There is a risk of graft-versus-host disease (GVHD), however, whereby mature lymphocytes entering with marrow recognize the new ... Alloreactive killer T cells, also called cytotoxic T lymphocytes (CTLs), have CD8 receptors that dock to the transplanted ...
Necrosis is increased in T lymphocytes.. Tingible body macrophages (TBMs) - large phagocytic cells in the germinal centers of ... Antibody binding subsequently spread to other epitopes. The similarity and cross-reactivity between the initial targets of nRNP ... B and T cell tolerance for apoptotic cells is abrogated, and the lymphocytes get activated by these autoantigens; inflammation ... The cytokines B-lymphocyte stimulator (BLys), interleukin 6, interleukin 17, interleukin 18, type I interferons, and tumor ...
HLAs corresponding to MHC class II (DP, DM, DOA, DOB, DQ, and DR) present antigens from outside of the cell to T-lymphocytes. ... SFVT categorization of HLA is applied in genetic association analysis so that the effects and roles of the epitopes shared by ... A representative cellular assay is the mixed lymphocyte culture (MLC) and used to determine the HLA class II types.[20] The ... This diversity enhances the survival of such groups, and thwarts evolution of epitopes in pathogens, which would otherwise be ...
"Colinear synthesis of an antigen-specific B-cell epitope with a promiscuous tetanus toxin T-cell epitope: a synthetic peptide ... Research done using phage display technology on lymphocytes from immunoinfertile men led to the isolation, characterization, ... One study examined the sperm-specific isozyme of human lactate dehydrogenase (LDH-C4) combined with a T-cell epitope to create ... "Autoimmunogenicity of the human sperm protein Sp17 in vasectomized men and identification of linear B cell epitopes". Fertil ...
Autoimune bolesti često se mogu pratiti do antitijela koja vezuju vlastite epitope tijela; mnogi se mogu otkriti krvnim ... "Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation". Contemporary Topics in Immunobiology ... Antigen obično sadrži različite epitope po svojoj površini raspoređenih diskontinuirano, a dominantni epitopi na određenom ... "Receptor editing in lymphocyte development and central tolerance". Nature Reviews. Immunology 6 (10): 728-740. 10.1038/nri1939 ...
By introducing these epitopes to the immune system, the vaccine aims to induce immune tolerance to gluten via introduction of ... It is common for lymphocytes and eosinophils to be seen. The bullae found in the skin affected by dermatitis herpetiformis are ... The vaccine contains three fragments, or epitopes, commonly found on gluten that are thought to be responsible for the pro- ... via pMHC-II in a process known as epitope spreading. Thus, the B cell presents the foreign peptide (modified gliadin) but ...
... such as lymphocytes or granulocytes.[8] ... Epitope *Linear. *Conformational. *Mimotope. *Antigen ...
... granular lymphocytes known today as NK cells. The demonstration that density gradient-isolated large granular lymphocytes were ... Expression of HLA-E at the cell surface is dependent on the presence of nonamer peptide epitope derived from the signal ... Natural killer cells, or NK cells, are a type of cytotoxic lymphocyte critical to the innate immune system. The role NK cells ... A functional marker of human non-T lymphocytes". Clinical and Experimental Immunology. 21 (2): 226-35. PMC 1538269. PMID 810282 ...
Boon T, van der Bruggen P (1996). "Human tumor antigens recognized by T lymphocytes". J Exp Med. 183: 725-29. PMID 8642276.. ... 2005). "Bcipep: a database of B-cell epitopes". BMC Bioinformatics. 6 (1): 79. PMID 15921533.. Pemeliharaan CS1: Banyak nama: ... Abbas A, Murphy K, Sher A (1996). "Functional diversity of helper T lymphocytes". Nature. 383 (6603): 787-93. PMID 8893001.. ... Sel T sitotoksik (Inggris: cytotoxic T lymphocyte, CTL) atau sel T pembunuh merupakan subkelompok dari sel T yang membunuh sel ...
Epitope, T-lymphocyte [r]: Sites on antigens that are recognized by T-lymphocytes. Antigenic determinants recognized and bound ... Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible ... Retrieved from "http://en.citizendium.org/wiki?title=Epitope,_T-lymphocyte&oldid=100601308" ...
B Lymphocyte Epitope. Antigenic Determinants recognized and bound by the B-Cell receptor. Epitopes recognized by the B-Cell ...
H-40, an antigen controlled by an Igh-linked gene and recognized by cytotoxic T-lymphocytes. I. Genetic analysis of H-40 and ... allowing to localize more than half of all the relevant H-2Dd serological epitopes to position 63 to 70. An alto CTL epitope of ... Gain and loss of serological and CTL epitopes specific for the H-2Dd and H-2Ld antigens were examined. The amino acid ... Mutagenesis Identifies Allo-Antigenic Epitopes of an H-2 Antigen Recognized by Antibodies and by Cytotoxic T-Lymphocytes. In: ...
MHC class I epitopes and validated these epitopes in vitro and in vivo. Cytotoxic T lymphocyte (CTL) epitopes within the HCV ... Epitope prediction methods are based on different algorithms and are instrumental for a first screening of possible epitopes. ... epitopes at close proximity to these CTL epitopes were analyzed using multiple prediction algorithms. This combined in silico ... analysis enhances the precision of identification of functional HCV-specific CTL epitopes. This approach will be applicable to ...
Loss of HIV-1-derived cytotoxic T lymphocyte epitopes restricted by protective HLA-B alleles during the HIV-1 epidemic.. ... alleles in a population and the number of cytotoxic T lymphocyte (CTL) escape mutations in epitopes restricted by those HLA ... In contrast, we found a significant loss of CTL epitopes restricted by HLA-B alleles associated with a low relative hazard of ... The number of CTL epitopes in HIV-1 variants restricted by the most common HLA alleles in the population did not change ...
In conclusion, this study describes novel HSV-2 epitopes eliciting strong CD8+ T cell responses that may facilitate epitope ... Peptide specific CD8+ human T cell lines from peripheral blood lymphocytes were generated from a HLA-A*02+ donor. High ... However, limited information has been available on CD8+ T cell epitopes or the functionality of antigen specific T cells during ... Nine previously uncharacterized CD8+ T cell epitopes were identified from HSV-2 infected BALB/c mice. HSV-2 specific peptide ...
Agonist peptide from a cytotoxic t-lymphocyte epitope of human carcinoembryonic antigen stimulates production of tc1-type ... The identification of an agonist peptide (YLSGADLNL, designated CAP1-6D) to an immunodominant cytotoxic T-lymphocyte (CTL) ... epitope (designated CAP1) of human carcinoembryonic antigen (CEA) has previously been reported. The agonist peptide harbors a ...
Immunological significance of cytotoxic T lymphocyte epitope variants in patients chronically infected by the hepatitis C virus ... This study was performed to test the hypothesis that cytotoxic T lymphocyte (CTL) selection of hepatitis C virus (HCV) escape ... Collectively, these observations suggest that CTL selection of epitope variants may have occurred in these patients before ...
HIV-1 gag-specific cytotoxic T lymphocytes recognize multiple highly conserved epitopes. Fine specificity of the gag-specific ... HIV-1 gag-specific cytotoxic T lymphocytes recognize multiple highly conserved epitopes. Fine specificity of the gag-specific ... HIV-1 gag-specific cytotoxic T lymphocytes recognize multiple highly conserved epitopes. Fine specificity of the gag-specific ... HIV-1 gag-specific cytotoxic T lymphocytes recognize multiple highly conserved epitopes. Fine specificity of the gag-specific ...
Cytotoxic T lymphocytes from HIV-1 seropositive individuals recognize immunodominant epitopes in Gp160 and reverse ... Cytotoxic T lymphocytes from HIV-1 seropositive individuals recognize immunodominant epitopes in Gp160 and reverse ... Cytotoxic T lymphocytes from HIV-1 seropositive individuals recognize immunodominant epitopes in Gp160 and reverse ... Cytotoxic T lymphocytes from HIV-1 seropositive individuals recognize immunodominant epitopes in Gp160 and reverse ...
PubMed journal article Identification and characterization of a human agonist cytotoxic T-lymphocyte epitope of human prostate- ... AnimalsApoptosisCarcinomaCells, CulturedCytokinesCytotoxicity, ImmunologicDendritic CellsEpitopes, T-LymphocyteFlow Cytometry ... Identification of a human CD8+ T lymphocyte neo-epitope created by a ras codon 12 mutation which is restricted by the HLA-A2 ... Identification and characterization of a human agonist cytotoxic T-lymphocyte epitope of human prostate-specific antigen.. Clin ...
AIDS virus-specific CD8+ T lymphocytes against an immunodominant cryptic epitope select for viral escape. Nicholas J. Maness, ... AIDS virus-specific CD8+ T lymphocytes against an immunodominant cryptic epitope select for viral escape ... Tat(28-35)SL8-specific CD8+ T lymphocytes are more effective than Gag(181-189)CM9-specific CD8+ T lymphocytes at suppressing ... T lymphocyte (CD8-TL) responses against a cryptic epitope, RHLAFKCLW (cRW9). Importantly, cRW9-specific CD8-TL selected for ...
Generation of Cytotoxic T Lymphocytes against Native and Altered Peptides of Human Leukocyte Antigen-A*0201 Restricted Epitopes ... Generation of Cytotoxic T Lymphocytes against Native and Altered Peptides of Human Leukocyte Antigen-A*0201 Restricted Epitopes ... Generation of Cytotoxic T Lymphocytes against Native and Altered Peptides of Human Leukocyte Antigen-A*0201 Restricted Epitopes ... Generation of Cytotoxic T Lymphocytes against Native and Altered Peptides of Human Leukocyte Antigen-A*0201 Restricted Epitopes ...
Cytotoxic T-Lymphocyte Epitopes Fused to Anthrax Toxin Induce Protective Antiviral Immunity. Amy M. Doling, Jimmy D. Ballard, ... 1998) Anthrax toxin mediated delivery in vivo and in vitro of a cytotoxic T-lymphocyte epitope from ovalbumin. Infect. Immun. ... 1989) Molecular analysis of a five-amino-acid cytotoxic T-lymphocyte (CTL) epitope: an immunodominant region which induces ... Cytotoxic T-Lymphocyte Epitopes Fused to Anthrax Toxin Induce Protective Antiviral Immunity ...
Use of Molecular Beacons for Rapid, Real-Time, Quantitative Monitoring of Cytotoxic T-Lymphocyte Epitope Mutations in Simian ... Use of Molecular Beacons for Rapid, Real-Time, Quantitative Monitoring of Cytotoxic T-Lymphocyte Epitope Mutations in Simian ... Use of Molecular Beacons for Rapid, Real-Time, Quantitative Monitoring of Cytotoxic T-Lymphocyte Epitope Mutations in Simian ... Use of Molecular Beacons for Rapid, Real-Time, Quantitative Monitoring of Cytotoxic T-Lymphocyte Epitope Mutations in Simian ...
Moreover, the antigen epitopes are strictly restricted to specific SLA molecules. In this study, we constructed SLA class I ... Moreover, the antigen epitopes are strictly restricted to specific SLA molecules. In this study, we constructed SLA class I ... The SLA-1*13:01, SLA-1*11:10, and SLA-1*11:01:02 proteins were able to bind specifically to different CTL epitopes of CSFV and ... The SLA-1*13:01, SLA-1*11:10, and SLA-1*11:01:02 proteins were able to bind specifically to different CTL epitopes of CSFV and ...
... and contains a Th lymphocyte, CTL, and two B lymphocyte epitopes (31). B lymphocyte epitopes also occur in the major homology ... the identification of Th lymphocyte epitopes in the same conserved EIAV p26 region as CTL (49) and B lymphocyte (12) epitopes ... were also found to contain Th lymphocyte epitopes. PBMC from H529 recognized both CTL and Th lymphocyte epitopes on peptides ... Promiscuous Th lymphocyte epitopes are candidates for inclusion in synthetic peptide vaccines directed against EIAV epitopes ...
We originally demonstrated that two virus-specific cytotoxic T-lymphocyte (CTL) epitopes, from the same virus but presented by ... A multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces ... A multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces ... A multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces ...
A*0201-restricted human immunodeficiency virus type 1 cytotoxic-T-lymphocyte epitope despite multiple variations in the epitope ... Identification of a novel HLA-A24-restricted cytotoxic T-lymphocyte epitope derived from HIV-1 Gag protein. AIDS12:2073-2074. ... Impaired Processing and Presentation of Cytotoxic-T-Lymphocyte (CTL) Epitopes Are Major Escape Mechanisms from CTL Immune ... Impaired Processing and Presentation of Cytotoxic-T-Lymphocyte (CTL) Epitopes Are Major Escape Mechanisms from CTL Immune ...
The AH1 Epitope Is the Immunodominant MHC Class I-restricted Antigen of TS/A Cells.. To test whether gp70-specific CTL ... 4B ⇓ , in vitro restimulation with tumor cells caused a gp70-specific CD8+ T-lymphocyte expansion; indeed, whereas TS/A-IL-4 ... Increasing numbers of AH1 peptide-coated 293Ld cells, but not 293Ld cells loaded with the irrelevant H-2 Ld P1A epitope, were ... 4A ⇓ , rejection of TS/A tumors was accompanied by gp70-specific CD8+ T-lymphocyte expansion that appeared more pronounced in ...
... restricted cytotoxic T-lymphocyte recognition epitopes in simian virus 40 T antigen ... Dissection of H-2Db-restricted cytotoxic T-lymphocyte epitopes on simian virus 40 T antigen by the use of synthetic peptides ... Prediction and analysis of HLA-A2/A24-restricted cytotoxic T-lymphocyte epitopes of the tumor antigen MAGE-n using the ... Sequential loss of cytotoxic T lymphocyte responses to simian virus 40 large T antigen epitopes in T antigen transgenic mice ...
Increased Breadth and Depth of Cytotoxic T Lymphocytes Responses against HIV-1-B Nef by Inclusion of Epitope Variant Sequences ... Increased breadth and depth of cytotoxic T lymphocytes responses against HIV-1-B Nef by inclusion of epitope variant sequences ... For all these strategies, a concern remains: how does HIV-1 diversity impact epitope recognition by the immune system? We ... studied the relationship between HIV-1 diversity and CD8\(^{+}\) T Lymphocytes (CTL) targeting of HIV-1 subtype B Nef using 944 ...
This study provides an up-to-date listing of currently known, well-defined viral CTL epitopes for HIV, EBV, CMV, HCV and HBV ... Assessment of Cytotoxic T Lymphocyte Responses Against Multiple Viral Infections by Combined Usage of Optimal Epitope Matrices ... assessment of cytotoxic T lymphocyte responses against multiple viral infections by combined usage of optimal epitope matrices ... while still allowing for the detailed determination of breadth and magnitude of virus-specific cytotoxic T lymphocyte (CTL) ...
Intranasal Immunization with Cytotoxic T-Lymphocyte Epitope Peptide and Mucosal Adjuvant Cholera Toxin: Selective Augmentation ... Intranasal Immunization with Cytotoxic T-Lymphocyte Epitope Peptide and Mucosal Adjuvant Cholera Toxin: Selective Augmentation ... Intranasal Immunization with Cytotoxic T-Lymphocyte Epitope Peptide and Mucosal Adjuvant Cholera Toxin: Selective Augmentation ... Intranasal Immunization with Cytotoxic T-Lymphocyte Epitope Peptide and Mucosal Adjuvant Cholera Toxin: Selective Augmentation ...
Identification of subdominant cytotoxic T lymphocyte epitopes encoded by autologous HIV type 1 sequences, using dendritic cell ... EpiVax Identifies T cell Epitopes critical to robust T cell immune response in SARS-CoV-2 , Results Published in NPJ Vaccines ...
A CD4+ cytotoxic T-lymphocyte clone to a conserved epitope on human immunodeficiency virus type 1 p24: cytotoxic activity and ... A*0201-restricted human immunodeficiency virus type 1 cytotoxic T-lymphocyte epitope despite multiple variations in the epitope ... A cd4 positive cytotoxic t lymphocyte clone to a conserved epitope on human immunodeficiency virus type 1 p24 cytotoxic ... Relative dominance of epitope-specific cytotoxic T-lymphocyte responses in human immunodeficiency virus type 1-infected persons ...
Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data. ... Free resource for searching and exporting immune epitopes. ...
Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data. ... Free resource for searching and exporting immune epitopes. ...
... thus defining an important T-cell epitope area of F. Within this same region, a nonamer peptide of F was found to be recognized ... and class II-restricted cytotoxic T lymphocytes (CTL). Genetically engineered F proteins and nested sets of synthetic peptides ... that human HLA class I and HLA class II-restricted CTL efficiently recognize nonapeptides or decapeptides representing epitopes ... Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles ...
  • Recently, novel bioinformatics approaches have identified a variety of immunogenic peptides derived from human tumor epitopes (21 , 22) that elicit cytotoxic T-cell responses. (aacrjournals.org)
  • In this study, we constructed SLA class I complexes in vitro comprising viral epitope peptides, the extracellular region of the SLA-1 molecules, and β2-microglobulin (β2m) using splicing overlap extension polymerase chain reaction (SOE-PCR). (frontiersin.org)
  • Specific binding of seven SLA-1 proteins to one classical swine fever virus (CSFV) and four porcine reproductive and respiratory syndrome virus (PRRSV) epitope peptides was detected by enzyme-linked immunosorbent assay (ELISA)-based method. (frontiersin.org)
  • The fixed combination of Asn 151 Val 152 residues was identified as the potentially key amino acid residues influencing the binding of viral several CTL epitope peptides to SLA-1 ∗ 13:01 and SLA-1 ∗ 04:01:01 proteins. (frontiersin.org)
  • The more flexible pocket E in the SLA-1 ∗ 13:01 protein might have fewer steric limitations and therefore be able to accommodate more residues of viral CTL epitope peptides, and may thus play a critical biochemical role in determining the peptide-binding motif of SLA-1 ∗ 13:01. (frontiersin.org)
  • Characterization of the binding specificity of peptides to SLA class I molecules provides an important basis for epitope studies of infectious diseases in swine, and for the rational development of novel porcine vaccines, as well as for detailed studies of CTL responses in pigs used as animal models. (frontiersin.org)
  • Using partially overlapping peptides, multideterminant and possibly promiscuous epitopes were identified within p26. (pubmedcentralcanada.ca)
  • Four additional peptides containing both CTL and Th lymphocyte epitopes were also identified. (pubmedcentralcanada.ca)
  • Multiple p15 peptides were shown to be reactive, but not all infected horses had Th lymphocytes recognizing p15 epitopes. (pubmedcentralcanada.ca)
  • The identification of peptides reactive with PBMC from outbred horses, some of which encoded both CTL and Th lymphocyte epitopes, should contribute to the design of synthetic peptide or recombinant vector vaccines for EIAV. (pubmedcentralcanada.ca)
  • As the minimal and optimal H-2Db-restricted CTL recognition sites have been defined by nonamer synthetic peptides, it is now possible to search for naturally processed H-2Db-restricted epitopes of T antigen and identify critical residues involved in processing, presentation, and recognition by SV40-specific CTL. (eurekamag.com)
  • We studied the relationship between HIV-1 diversity and CD8\(^{+}\) T Lymphocytes (CTL) targeting of HIV-1 subtype B Nef using 944 peptides (10-mers overlapping by nine amino acids (AA)) that corresponded to consensus peptides and their most common variants in the HIV-1-B virus population. (harvard.edu)
  • The recognition of the HLA-DQ-restricted epitope by this clone was further defined by using overlapping synthetic peptides. (eurekamag.com)
  • Epitopes described in Dissection of H-2Db-restricted cytotoxic T-lymphocyte epitopes on simian virus 40 T antigen by the use of synthetic peptides and H-2Dbm mutants. (iedb.org)
  • Identification of HLA-A24 restricted pre-erythrocytic stage specific T-cell epitopes using Plasmodium falciparum synthetic peptides: a preliminary study. (semanticscholar.org)
  • Using a strategy involving the in vitro stimulation of peripheral blood mononuclear cells (PBMC) with HBV-derived synthetic peptides containing HLA-A2.1, -A31, and -Aw68 binding motifs, we have previously described CTL responses to several epitopes within the HBV nucleocapsid and envelope antigens in patients with acute hepatitis. (rupress.org)
  • Importantly, the peptide-activated CTL recognize target cells that express endogenously synthesized polymerase protein, suggesting that these peptides represent naturally processed viral epitopes. (rupress.org)
  • The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides. (ox.ac.uk)
  • Peptides are typically too short to have a well-defined fold, yet sometimes can simulate the epitope, binding to antibodies. (proteopedia.org)
  • In the absence of the crystal structure of an antibody:antigen complex, a common way to identify the epitope recognized by a particular antibody is to display random peptides (for example, using phage display libraries ), and then to identify the sequences of the peptides with the highest affinity for the antibody. (proteopedia.org)
  • The Pepitope Server predicts epitopes on the surface of a 3D protein antigen model, based on a list of peptides that bind to the antibody. (proteopedia.org)
  • To identify epitopes recognized by alloreactive CTL we have examined H-2Kb-specific CTL for their recognition of synthetic peptides with sequences derived from the native Kb class I molecule. (scripps.edu)
  • Peptides encompassing this epitope-stimulated GAD65-specific T cells from diabetic patients and a DR4-positive individual at high risk for developing IDDM. (pnas.org)
  • A separate study, also using DR4-transgenic mice, found that these same three epitopes also were included in immunodominant regions (116-130, 271-285, and 551-565) when the GAD65 protein, rather than the peptides, was used as the immunogen ( 17 ). (pnas.org)
  • We now report the direct identification of one of these epitopes by liquid chromatography-electrospray ionization mass spectrometric (LC/MS) analysis of peptides bound by DR4 molecules on human antigen-presenting cells (APCs). (pnas.org)
  • A previous screening of the chlamydial genome for putative HLA-B27 ligands predicted multiple peptides that were recognized in vitro by CD8 + T-lymphocytes from patients. (mcponline.org)
  • Epitope optimization usually requires numerous truncated peptides or a reverse immunogenetic approach, where the peptide binding motif is used to predict epitopes. (ox.ac.uk)
  • These binding motifs do not reliably predict all peptides which are CTL epitopes. (ox.ac.uk)
  • Comparison of 24 peptides eluted from HLA-B8 with 10 HLA-B8-restricted defined CTL epitopes demonstrated that known epitopes varied considerably at anchor positions. (ox.ac.uk)
  • Our results demonstrate that the HER2/neu- encoded HLA-A2.1-associated epitopes recognized by CTLs are presented as naturally processed peptides on gastric cancer lines. (elsevier.com)
  • Experimental Design: The amino acid sequence of CEA was examined for the presence of potential T helper epitopes, and candidate peptides were used to stimulate in vitro T-cell responses. (elsevier.com)
  • The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides (reprinted from Cell, vol. 44, pg. (ox.ac.uk)
  • To assess the immunodominance patterns of HIV-1-specific cytotoxic T lymphocyte (CTL) responses and the contribution of these responses against the peptides scanning optimal epitopes in chronic infection, we test the HIV-1-specific CTL responses against a panel of 413 overlapping peptides spanning HIV-1 Asian B sequence, including 147 peptides corresponding to optimal clade B epitopes in 49 chronically HIV-1 infected individuals by interferon-γ Elispot assay. (eurekaselect.com)
  • A sequence pattern for peptides presented to cytotoxic T lymphocytes by HLA B8 revealed by analysis of epitopes and eluted peptides. (ox.ac.uk)
  • The dominant epitope was mapped using recombinant vaccinia viruses that expressed fragments of the NP and then synthetic peptides based on the NP amino acid sequence. (ox.ac.uk)
  • The amino acid sequence of the peptide epitope was compared to six other known virus peptides known to be restricted by HLA B8 and a sequence homology was identified, which predicted nonamer and octamer epitope sequences. (ox.ac.uk)
  • One of the predicted epitope peptides was made and shown to be recognized by specific CTL. (ox.ac.uk)
  • the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)
  • Tetramer-binding data also show that CD8+ T lymphocytes from monkeys immunized with mosaic immunogen have a trend of higher binding to majority of the variant peptides tested. (biomedcentral.com)
  • Herein, we used a novel, comprehensive peptide panel containing all possible 8-, 9- and 10-mer peptides spanning the RSV fusion protein to screen for novel HLA-restricted T-cell epitopes. (vanderbilt.edu)
  • Cytotoxic T lymphocytes from HIV-1 seropositive individuals recognize immunodominant epitopes in Gp160 and reverse transcriptase. (jimmunol.org)
  • There was some concern that the combination of several normally immunodominant epitopes might result in a new hierarchy of dominance, in which certain epitopes predominated and others exhibited reduced immunogenicity. (asm.org)
  • Despite limited data supporting the superiority of dominant over subdominant responses, immunodominant epitopes represent the preferred vaccine candidates. (washington.edu)
  • CD4 T cell epitope mapping, analyses of immunodominant epitopes in the context of the three-dimensional structures of flavivirus proteins, class II allele-specific affinity predictions and informatics-based approaches as well as in vitro T cell activation assays using structurally defined antigen preparations. (meduniwien.ac.at)
  • Responses against such optimally defined epitopes can account for a significant part of the total virus-specific immune responses, especially when they represent immunodominant epitopes covering the most immunogenic proteins of specific viral genomes. (biomedcentral.com)
  • CTL recognize infected cells when peptide epitopes (usually 8 to 10 amino acids) derived from these parasites are presented by molecules encoded by the host class I major histocompatibility complex (MHC-I) ( 17 ). (asm.org)
  • The HER2/neu specificity of these gastric cancer-specific CTLs was demonstrated using HER2/neu-transfected cell lines and HER2/neu-expressing tumors, and with a set of HER2/neu-derived peptide epitopes. (elsevier.com)
  • Although many CEA peptide epitopes capable of stimulating CTLs have been identified, no MHC class II-restricted T helper epitope has yet been reported. (elsevier.com)
  • Although a limited number of immunodominant peptide epitopes are consistently observed in diseases such as HIV-1 infection, the relationship between immunodominance and antigen processing in humans is largely unknown. (jci.org)
  • Sites on antigens that are recognized by T-lymphocytes . (citizendium.org)
  • Gain and loss of serological and CTL epitopes specific for the H-2D d and H-2L d antigens were examined. (springer.com)
  • They mainly bind and present antigens of intracellular origin to circulating MHC I-restricted cytotoxic T lymphocytes (CTLs). (frontiersin.org)
  • It is unfortunate that epitope has caught on as the term to describe the peptide fragments that T cells recognize, since these are not necessarily derived from the surfaces of protein antigens, but may be derived from portions that were buried in the folded protein. (proteopedia.org)
  • MHC II-presented human cancer epitopes, like their MHC I counterparts, have turned out to be a mixture of unmutated cancer testis antigens ( 19 - 21 ), differentiation antigens ( 22 , 23 ), and mutated unique antigens restricted to an individual cancer ( 24 - 26 ). (pnas.org)
  • This result prompted us to identify the conserved epitopes that are utilized as selecting antigens and the mechanism of their enhanced immunogenicity. (nature.com)
  • High-throughput epitope discovery reveals frequent recognition of neo-antigens by CD4 + T cells in human melanoma. (nature.com)
  • Several of the identified T-cell epitopes share similarity with common bacterial and viral antigens, suggesting the involvement of pre-existing microbial cross-reactive T cells in rapid and durable tumour regression seen in some patients. (bireme.br)
  • Determinants on major histocompatibility complex class I molecules recognized by cytotoxic T-lymphocytes. (springer.com)
  • 1984. H-40, an antigen controlled by an Igh-linked gene and recognized by cytotoxic T-lymphocytes. (springer.com)
  • Tumor vaccine design requires prediction and validation of immunogenic MHC class I epitopes expressed by target cells as well as MHC class II epitopes expressed by antigen-presenting cells essential for the induction of optimal immune responses. (mdpi.com)
  • In conclusion, this study describes novel HSV-2 epitopes eliciting strong CD8+ T cell responses that may facilitate epitope based vaccine design and aid immunomonitoring of antigen specific T cell frequencies in preclinical and clinical settings. (mdpi.com)
  • The identification of multiple relatively conserved epitopes in the HIV-1 gag protein and the heterogeneity of CTL responses to this protein may have important implications for vaccine development and our understanding of AIDS pathogenesis. (jimmunol.org)
  • Here, we show that Mamu-B*17 + simian immunodeficiency virus (SIV)mac239-infected rhesus macaques that spontaneously controlled viral replication consistently made strong CD8 + T lymphocyte (CD8-TL) responses against a cryptic epitope, RHLAFKCLW (cRW9). (rupress.org)
  • Immune responses directed against cryptic epitopes have been detected in several infections, including influenza virus ( 12 ), murine AIDS ( 13 ), and, importantly, HIV ( 14 ). (rupress.org)
  • Although these experiments elegantly demonstrated the existence of HIV-derived cryptic epitope-specific CD8-TLs, the importance of these responses in HIV-infected humans or SIV-infected macaques has not yet been addressed. (rupress.org)
  • Here, we show that Mamu-B*17 + macaques mounted strong, chronic phase responses against a cryptic epitope, RHLAFKCLW (cRW9). (rupress.org)
  • Antigen-specific T-helper (Th) lymphocytes are critical for the development of antiviral humoral responses and the expansion of cytotoxic T lymphocytes (CTL). (pubmedcentralcanada.ca)
  • The development of immune responses capable of recognizing conserved epitopes expressed on virus and viral-infected cells is critical to the control of virus replication and therefore clinical disease. (pubmedcentralcanada.ca)
  • Synthetic peptide vaccines have numerous advantages including the inclusion of only defined epitopes to generate relevant immune responses rather than a broad response that may exacerbate infectivity and disease ( 4 , 23 , 29 , 48 ). (pubmedcentralcanada.ca)
  • A multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces appropriate responses in vivo and confers protection against more than one pathogen. (asm.org)
  • Thus, the development of assay systems, which optimize cell usage, while still allowing for the detailed determination of breadth and magnitude of virus-specific cytotoxic T lymphocyte (CTL) responses, is urgently needed. (harvard.edu)
  • Furthermore when combining T cell expansion and RecycleSpot with the use of rationally designed peptide matrices, antiviral immunity against more than 400 different CTL epitopes from five different viruses can be reproducibly assessed from samples of less than 10 milliliters of blood without compromising information on the breadth and magnitude of these responses. (harvard.edu)
  • HLA-A*0201 restricted CD8+ T-lymphocyte responses to malaria: identification of new Plasmodium falciparum epitopes by IFN-gamma ELISPOT. (semanticscholar.org)
  • Furthermore, CTL responses against polymerase, core, and envelope epitopes were detectable for more than a year after complete clinical recovery and seroconversion, reflecting either the persistence of trace amounts of virus or the presence of long lived memory CTL in the absence of viral antigen. (rupress.org)
  • However, although in vivo lymphocyte expansion has been correlated with good clinical outcome following DLI, the specificity and functional characteristics of EBV-specific T-cell responses remain poorly characterized. (frontiersin.org)
  • HIV-1-specific cytotoxic T-lymphocyte (CTL) responses have been detected at a low frequency in many HIV-1-exposed, persistently seronegative (HEPS) subjects. (ox.ac.uk)
  • To address this issue we studied CD8(+) lymphocyte responses to a panel of HIV-1 CTL epitopes in 91 HEPS and 87 HIV-1-infected Nairobi sex workers. (ox.ac.uk)
  • HIV-specific responses in seropositive women focused strongly on epitopes rarely or never recognized in HEPS subjects, who targeted epitopes that were subdominant or unrecognized in infected women. (ox.ac.uk)
  • To address the function of subdominant responses in human immunodeficiency virus infection, we analyzed cytotoxic T lymphocyte responses restricted by HLA-B 1503, a rare allele in a cohort infected with clade B, although common in one infected with clade C. HLA-B 1503 was associated with reduced viral loads in the clade B cohort but not the clade C cohort, although both shared the immunodominant response. (washington.edu)
  • Clade B viral control was associated with responses to several subdominant cytotoxic T lymphocyte epitopes, whereas their clade C variants were less well recognized. (washington.edu)
  • These data suggest that subdominant responses can contribute to in vivo viral control and that high HLA allele frequencies may drive the elimination of subdominant yet effective epitopes from circulating viral populations. (washington.edu)
  • This is, to our knowledge, the first endogenously processed epitope involved in HLA-B27-restricted responses against C. trachomatis in reactive arthritis. (mcponline.org)
  • Because of the likely involvement of HLA-B27 in the pathogenesis of chronically evolving ReA, the role of CD8 + T-cell responses in the protective immunity against C. trachomatis and the presence of HLA-B27-restricted T-cells in patients with Chlamydia -induced ReA, the identification of relevant chlamydial epitopes becomes crucial to establish the pathogenetic mechanism of this disease. (mcponline.org)
  • Results: We describe here that using an algorithm to identify promiscuous helper T-cell epitopes, a peptide of CEA occupying residue positions 653 to 667 (CEA 653-667 ), was effective in inducing in vitro T helper responses in the context of the HLA-DR4, HLA-DR7, and HLA-DR9 alleles. (elsevier.com)
  • Interestingly, the newly identified helper T-cell epitope was found to overlap with a previously described HLA-A24-restricted CTL epitope, CEA 652-660 , which could facilitate the development of a therapeutic vaccine capable of eliciting both CTL and T helper responses in patients suffering from epithelial carcinomas. (elsevier.com)
  • Virus-specific cytotoxic T-lymphocyte (CTL) responses are critical in the control of human immunodeficiency virus type 1 (HIV-1) infection and will play an important part in therapeutic and prophylactic HIV-1 vaccines. (ox.ac.uk)
  • Two of the novel epitopes were immunodominant among the HLA-A2-restricted CTL responses of individuals with acute and chronic HIV-1 infection. (ox.ac.uk)
  • The novel CTL epitopes identified here should be included in future vaccines designed to induce HIV-1-specific CTL responses restricted by the HLA-A2 superfamily and will be important to assess in immunogenicity studies in infected persons and in uninfected recipients of candidate HIV-1 vaccines. (ox.ac.uk)
  • We present detailed studies of human immunodeficiency virus (HIV)-specific cytotoxic T-lymphocyte (CTL) responses to clade A or C HIV type 1 in three donors infected in East Africa. (ox.ac.uk)
  • These data demonstrate that the consistent immunodominance patterns of HIV-1-specific CTL responses of Chinese HIV-1 infected individuals and an inverse correlation between the relative contribution of responses restricted by HLA alleles and viral load, which indicates the important protective effect of optimal epitopes against slow disease progression even in advanced infection. (eurekaselect.com)
  • The data show that HLA-B63-positive individuals indeed mounted responses to previously identified HLA-B57-restricted epitopes as well as towards novel, HLA-B63-restricted CTL targets that, in turn, can be presented by HLA-B57 and HLA-B58. (ox.ac.uk)
  • Here, we measured the functional avidities of HIV-specific CTL responses targeting 20 different, optimally defined CTL epitopes restricted by 13 different HLA class I alleles in a cohort comprising 44 HIV controllers and 68 HIV non-controllers. (cf.ac.uk)
  • We study the virus-specific immune responses with a special emphasis on the fine-specificities of CD4 T helper cells that recognize epitopes in the viral structural proteins and thus can provide direct help to B cells producing neutralizing and protective antibodies. (meduniwien.ac.at)
  • 2014. Specificities of human CD4+ T cell responses to an inactivated flavivirus vaccine and infection: correlation with structure and epitope prediction. (meduniwien.ac.at)
  • This early, germline cross-reactivity is associated with B cell antigen receptor (BCR)-mediated recognition of variable epitopes with low affinity and supported by the intriguing findings of McGargill's group showing that limiting the generation of highly specific IgG B cells by rapamycin treatment enhanced the breadth of protective antibody responses to influenza 6 . (nature.com)
  • By monitoring B cell responses targeting conserved HA epitopes in mice, we previously demonstrated that influenza virus infection induces ectopic formation of lung GCs at the viral replication site, where cross-reactive B cells are selected at increased frequencies 12 . (nature.com)
  • Immunizations of nonhuman primates using consensus and mosaic immunogens have been shown to elicit cross-reactive CD8+T lymphocyte responses that increase the depth of epitope recognition. (biomedcentral.com)
  • Our data show that Env immunizations can generate cross-reactive CD8+T lymphocytes that recognize 2 of 9 (22%) of the variants of p41A epitope, with higher responses induced by the consensus and the 3-valent mosaic immunogens (variant from clade C and variant from clade A/E) compared to the single clade Env immunogen. (biomedcentral.com)
  • Therefore, we wanted to test the impact that vaccine-induced CTL responses against an immunodominant Gag epitope might have in the absence of other immune responses. (elsevier.com)
  • Cytotoxic T lymphocyte (CTL) epitopes within the HCV non-structural proteins were identified, and proteasomal cleavage sites and helper T cell (Th) epitopes at close proximity to these CTL epitopes were analyzed using multiple prediction algorithms. (mdpi.com)
  • The epitopes are derived from cytoplasmic proteins cleaved by the proteosome into small peptide fragments. (asm.org)
  • The SLA-1 ∗ 13:01, SLA-1 ∗ 11:10, and SLA-1 ∗ 11:01:02 proteins were able to bind specifically to different CTL epitopes of CSFV and PRRSV and the MHC restrictions of the five epitopes were identified. (frontiersin.org)
  • This study describes Th lymphocyte reactivity in EIAV carrier horses to two proteins, p26 and p15, encoded by the relatively conserved EIAV gag gene. (pubmedcentralcanada.ca)
  • Efficacious lentiviral vaccines designed to induce cytotoxic T lymphocytes (CTL) in outbred populations with a diverse repertoire of MHC class I molecules should contain or express multiple viral proteins. (microbiologyresearch.org)
  • To determine the equine infectious anaemia virus (EIAV) proteins with epitopes most frequently recognized by CTL from seven horses infected for 0·5 to 7 years, retroviral vector-transduced target cells expressing viral proteins were used in CTL assays. (microbiologyresearch.org)
  • Antibody epitopes can occur on the surfaces of native folded proteins, or equally well on denatured conformations of proteins. (proteopedia.org)
  • Therefore, we genetically fused a minigene encompassing the NY-ESO-1 cytotoxic T lymphocyte (CTL) epitope 157-165 (ESO p157-165) to the human heat shock cognate protein 70 (hsc70) and expressed the resulting fusion proteins in Escherichia coli. (elsevier.com)
  • The precise target-cell specificity in this disease implies the existence of antigenic self proteins derived from beta cells that are recognized by autoimmune T lymphocytes. (pnas.org)
  • Here stable transfectants expressing bacterial fusion proteins in human cells were generated to investigate the endogenous processing and presentation by HLA-B27 of two such epitopes through comparative immunoproteomics of HLA-B27-bound peptide repertoires. (mcponline.org)
  • Thus, expression and intracellular processing of chlamydial proteins into human cells allowed us to identify two bacterial HLA-B27 ligands, including the first endogenous T-cell epitope from C. trachomatis involved in spondyloarthropathy. (mcponline.org)
  • That human proteins contain sequences mimicking chlamydial T-cell epitopes suggests a basis for an autoimmune component of Chlamydia -induced HLA-B27-associated disease. (mcponline.org)
  • Based on the known crystallographic structures of flavivirus proteins, immunodominant regions of T cell epitopes have been mapped to specific structural domains in the context of human infections as well as vaccination with different flavivirus vaccines. (meduniwien.ac.at)
  • Analyses with segmental reassortants of lymphocytic choriomeningitis virus (LCMV) RNA have shown that cytotoxic T lymphocytes (CTL) are induced by and recognize proteins encoded by the viral short segment, which specifies two virus structural proteins, glycoprotein (GP) and nucleoprotein (NP). (scripps.edu)
  • Background Previous small studies have demonstrated positive immunohistochemical staining in rabbit and human atherosclerotic plaques by antibodies that recognize oxidized low-density lipoprotein (OxLDL), but none have examined a large number of human coronary arteries or evaluated whether epitopes recognized by these antibodies might be present on plaque proteins other than OxLDL. (ahajournals.org)
  • Extracellular Ox5 staining colocalized with apo B, but cell-associated Ox5 staining occurred in the absence of cell-associated apo B staining, which suggests that cell-associated epitopes for Ox5 were on proteins other than LDL. (ahajournals.org)
  • Thus, epitopes for Ox5 can form on proteins other than apo B. Also, phorbol ester-treated macrophages cultured in apo B-free medium developed epitopes for Ox5. (ahajournals.org)
  • First, some of the "anti-OxLDL" antibodies used in these studies can recognize oxidation epitopes on proteins other than apo B, 31 32 which demonstrates that these antibodies are not specific for OxLDL alone. (ahajournals.org)
  • Instead, immune analyses need either to be restricted to a selected number of specific viral proteins, or to the use of previously defined, optimal CTL epitopes. (biomedcentral.com)
  • Here we demonstrate that antiviral immunity can be induced in BALB/c mice immunized with PA plus a fusion protein containing the N-terminal 255 amino acids of LF (LFn) and an epitope from the nucleoprotein (NP) of lymphocytic choriomeningitis virus. (asm.org)
  • Biology of cloned cytotoxic T lymphocytes specific for lymphocytic choriomeningitis virus: clearance of virus in vivo. (microbiologyresearch.org)
  • Following infection of the H-2(d) mouse by lymphocytic choriomeningitis virus, the newly generated cytotoxic T lymphocyte (CTL) response is focused to a single 9-amino-acid peptide sequence (epitope) of the virus. (elsevier.com)
  • More than 96% of the primary, secondary, and clonal CTL respond to this lymphocytic choriomeningitis virus nucleoprotein epitope. (elsevier.com)
  • One strategy to enhance the immunogenicity of a self-antigen such as PSA is to develop agonist epitopes that are potentially more immunogenic. (unboundmedicine.com)
  • In the study, we extended this approach, which we termed string of beads, expanding the immunogenic scope in two ways: first, by introduction of T helper (Th) and B-cell (antibody) epitopes alongside CTL epitopes and second, by including immunogenic sequences from a variety of infectious agents, five viruses and one bacterium. (asm.org)
  • There are two immunogenic sites on the type A influenza A/Japan/57 (H2N2) hemagglutinin (HA) that can be recognized by class I major histocompatibility complex (MHC), H-2Kd-restricted cytolytic T lymphocytes (CTLs). (rupress.org)
  • Indeed, three of these candidate autoantigen epitopes, corresponding to residues 115-127, 274-286, and 554-566 of hGAD65, were immunogenic when used to immunize mice transgenic for HLA-DR4 ( 16 ). (pnas.org)
  • Chlamydia -specific CD8 + T-cells are found in reactive arthritis patients, but the immunogenic epitopes are unknown. (mcponline.org)
  • As HBV core protein (HBc) is the most conservative and immunogenic component, in this study we used an overlapping 9-mer peptide pool covering HBc to screen and identify specific CTL epitopes. (protein-cell.org)
  • Germinal center (GC) B cells at viral replication sites acquire specificity to poorly immunogenic but conserved influenza hemagglutinin (HA) epitopes. (nature.com)
  • Protective cross-reactivity is also acquired by targeting conserved viral epitopes that are often immunosubdominant and poorly immunogenic. (nature.com)
  • MARIA allows identification of immunogenic epitopes in diverse cancers and autoimmune disease. (nature.com)
  • One of these sites encompasses two distinct partially overlapping epitopes, which span HA residues 204-212 and 210-219. (rupress.org)
  • The VH epitope recognized by clone 40-2 could be mapped to a 10 amino acid peptide spanning MOPC 21 VH residues 49-58. (rupress.org)
  • The first epitope, recognized by only two of the clones, lies within GP residues 1 to 218. (scripps.edu)
  • The structures reveal virus conformational changes, the Fab-binding mode to the capsid, the residues comprising the epitope and indicate a potential interaction of U4 with the minor structural protein, L2. (bioportfolio.com)
  • From these results we conclude that the amino acid sequence encompassing from position 63 to 70 of the H-2D d and H-2L d molecules forms major alto- antigenic epitopes recognized by multiple antibodies and CTLs. (springer.com)
  • Binding of an appropriate epitope to an SLA class I molecule is the single most selective event in antigen presentation and the first step in the killing of infected cells by CD8+ CTLs. (frontiersin.org)
  • Investigating escape mechanisms of human immunodeficiency virus type 1 (HIV-1) from cytotoxic T lymphocytes (CTLs) is essential for understanding the pathogenesis of HIV-1 infection and developing effective vaccines. (asm.org)
  • Accumulated evidence has indicated a critical role of cytotoxic T lymphocytes (CTLs) in controlling human immunodeficiency virus (HIV) replication during acute and chronic infection ( 16 ). (asm.org)
  • This unique system affords the opportunity to evaluate the T-cell response to a single viral CTL epitope in a case in which the outcome of infection, either viral clearance or host death, is mediated by the CTLs. (elsevier.com)
  • Specifically, the molecular structure of the T-cell receptors (TCRs) of CTLs responding to this epitope was analyzed. (elsevier.com)
  • 50 nM) affinity, were able to sensitize HLA-A2 + CIR/A2 cells to be recognized by the gastric cancer-specific CTLs, demonstrating the immunodominance of these epitopes. (elsevier.com)
  • However, limited information has been available on CD8+ T cell epitopes or the functionality of antigen specific T cells during infection or following immunization with experimental vaccines. (mdpi.com)
  • CD8 + T lymphocytes (CD8-TLs) play a critical role in control of HIV and simian immunodeficiency virus (SIV) infection. (rupress.org)
  • Cytotoxic T lymphocytes (CTL) are thought to contribute to viral clearance and liver cell injury during hepatitis B virus (HBV) infection. (rupress.org)
  • In this study we define six HLA-A2-restricted CTL epitopes located in the highly conserved reverse transcriptase and RNase H domains of the viral polymerase protein, and we show that the CTL response to polymerase is polyclonal, multispecific, and mediated by CD8+ T cells in patients with acute viral hepatitis, but that it is not detectable in patients with chronic HBV infection or uninfected healthy blood donors. (rupress.org)
  • These differences in epitope specificity were restricted by only those HLA class I alleles that are associated with a reduced risk of HIV-1 infection in this cohort. (ox.ac.uk)
  • The association between differential recognition of distinct CTL epitopes and protection from HIV-1 infection may have significant implications for vaccine design. (ox.ac.uk)
  • Abstract: Most neutralizing antibodies elicited during influenza virus infection or by vaccination have a narrow spectrum because they usually target variable epitopes in the globular head region of hemagglutinin (HA). (osti.gov)
  • Several HLA class I alleles have been associated with slow human immunodeficiency virus (HIV) disease progression, supporting the important role HLA class I-restricted cytotoxic T lymphocytes (CTL) play in controlling HIV infection. (ox.ac.uk)
  • The data support an important role of the presented epitope in mediating relative control of HIV replication and help to better define immune correlates of controlled HIV infection. (ox.ac.uk)
  • Virus-specific cytotoxic T lymphocytes (CTL) with high levels of functional avidity have been associated with viral clearance in hepatitis C virus infection and enhanced antiviral protective immunity in animal models. (cf.ac.uk)
  • 2018. Structural influence on the dominance of virus-specific CD4 T cell epitopes in Zika virus infection. (meduniwien.ac.at)
  • Virus-specific cytotoxic T lymphocytes (CTL) play a major role in the clearance of respiratory syncytial virus (RSV) infection. (vanderbilt.edu)
  • To begin monitoring the immunological response to infection, especially in infants, it is important to identify human leucocyte antigen (HLA)-restricted CTL epitopes. (vanderbilt.edu)
  • Identification of relevant Th lymphocyte epitopes remains an important step in the development of an efficacious subunit peptide vaccine against equine infectious anemia virus (EIAV), a naturally occurring lentivirus of horses. (pubmedcentralcanada.ca)
  • The conceptual difference between the theories regarding the maintainance of polymorphism in CTL epitopes may have significant implication for vaccine design. (mysciencework.com)
  • In conclusion, our findings implicate HER2/neu-derived epitopes as potential candidates for novel immunotherapy and vaccine strategies against gastric cancer. (elsevier.com)
  • Our results identify a class of cross-protective antibodies that are selected at the viral replication site, and provide insights into vaccine strategies using the occluded epitope. (nature.com)
  • However, one of the limitations of vaccine-induced epitope-specific CD8+T lymphocytes includes lack of protection against diverse strains and emergent forms of HIV-1 due to altered T cell receptor (TCR) affinity for variant peptide:MHC class I complexes. (biomedcentral.com)
  • In this study, we immunized a cohort of fifteen Mamu-A*01+ rhesus monkeys with either a 3-valent mosaic Env, group M consensus Env, or single clade B Env vaccine and compared the ability of the CD8+T lymphocyte populations elicited by each immunogen to recognize variants of an HIV-1 envelope epitope sequence p41A (YI9). (biomedcentral.com)
  • We identified vaccine-induced CD8+T lymphocytes populations using tetramers constructed with 9 variants of p41A epitope. (biomedcentral.com)
  • Vaccine-induced cytotoxic T lymphocytes (CTL) have been implicated in the control of virus replication in simian immunodeficiency virus (SIV)-challenged and simian-human immunodeficiency virus-challenged macaques. (elsevier.com)
  • Analysis of gag epitope recognition using cloned CTL demonstrated heterogeneity and specificity not appreciated using unstimulated PBMC. (jimmunol.org)
  • To study the processing and presentation of known CTL epitopes, we prepared Epstein-Barr virus-transformed B cells that endogenously express the gag gene of six field isolates by adopting an env/nef -deletion HIV-1 vector pseudotyped with vesicular stomatitis virus G protein and then tested them for the recognition by Gag epitope-specific CTL lines or clones. (asm.org)
  • For all these strategies, a concern remains: how does HIV-1 diversity impact epitope recognition by the immune system? (harvard.edu)
  • Recognition of an immunoglobulin VH epitope by influenza virus-specific class I major histocompatibility complex-restricted cytolytic T lymphocytes. (rupress.org)
  • This direct identification and manipulation of GAD65 epitope recognition provides an approach toward dissection of the complex CD4 + T cell response in IDDM. (pnas.org)
  • Distinct recognition of non-clade B human immunodeficiency virus type 1 epitopes by cytotoxic T lymphocytes generated from donors infected in Africa. (ox.ac.uk)
  • Although cross-clade CTL recognition of these epitopes does occur, recognition can also be highly clade specific. (ox.ac.uk)
  • Peptide presentation is critical for immune recognition of pathogen-infected cells by CD8 + T lymphocytes. (jci.org)
  • CTL recognition of a previously defined common vital mutation in an immunodominant SIVmac Gag epitope was evaluated. (elsevier.com)
  • So you could try washing your stimulator cells before adding responders or you might have to add a peptide for lymphocyte recognition and response! (protocol-online.org)
  • The highly detailed epitopes identified for the two antibodies provide a framework for continuing biochemical, genetic and biophysical studies. (bioportfolio.com)
  • During the past 30 years my laboratory has generated 40+ monoclonal antibodies (mAbs) directed to structural and conformational epitopes on human ACE as well as ACE from rats, mice and other species. (bioportfolio.com)
  • Here, high-throughput epitope mapping of local GC B cells is used to identify conserved HA epitope selecting cross-reactive antibodies that mediate heterosubtypic protection. (nature.com)
  • Importantly, systemic immunization by the post-fusion HA antigen results in GC B cells targeting the occluded epitope, and induces a class of protective antibodies that have cross-group specificity and afford protection independent of virus neutralization activity. (nature.com)
  • Extensive analyses of HIV broadly neutralizing antibodies (bNAbs) has highlighted the requirement for extraordinary frequencies of V(D)J mutations and repeated rounds of selection to acquire the requisite affinity/specificity for conserved, immunosubdominant neutralizing epitopes 8 . (nature.com)
  • Thus, V(D)J mutations and selection during the GC pathway fine-tune the specificity and affinity of the antibodies to conserved influenza epitopes. (nature.com)
  • WW.B1 recognized PBMC and the Jurkat T leukemic cell line, immunoprecipitated both H.MBP and a complex indistinguishable from CD3, and possessed the same biologic properties - induction of T lymphocyte proliferation and inhibition of CTL function - as commercially available anti-CD3 antibodies. (elsevier.com)
  • Methods and Results Immunohistochemistry was performed on atherosclerotic (n=87) and nonatherosclerotic (n=51) coronary arterial segments from 20 patients by use of monoclonal antibodies that recognize epitopes on macrophages, smooth muscle cells, apolipoprotein (apo) B, and OxLDL. (ahajournals.org)
  • 28 29 Also, immunohistochemical studies have used a variety of antibodies that recognize epitopes on OxLDL to demonstrate the presence of these epitopes in rabbit 30 31 32 33 34 and human 35 36 atherosclerotic lesions. (ahajournals.org)
  • This combined in silico analysis enhances the precision of identification of functional HCV-specific CTL epitopes. (mdpi.com)
  • The identification of an agonist peptide (YLSGADLNL, designated CAP1-6D) to an immunodominant cytotoxic T-lymphocyte (CTL) epitope (designated CAP1) of human carcinoembryonic antigen (CEA) has previously been reported. (nih.gov)
  • Identification of multiple epitopes and characterization of patterns of cytokine release. (iedb.org)
  • Identification of a new HLA-A2-restricted T-cell epitope within HM1.24 as immunotherapy target for multiple myeloma. (semanticscholar.org)
  • Identification and genetics of horse lymphocyte alloantigens. (microbiologyresearch.org)
  • Most such efforts have been directed to identification of MHC I-presented cancer epitopes recognized by CD8 + T lymphocytes ( 10 - 18 ), with a considerably smaller number of MHC II-presented epitopes recognized by CD4 + T lymphocytes having been identified and characterized. (pnas.org)
  • Although the human cancers are the obvious targets of immunotherapy, identification of antigenic epitopes of murine cancers permits their use in experimental models that allow far more experimental flexibility in a far shorter time than do clinical trials with humans ( 28 ). (pnas.org)
  • Identification of a novel HLA-A24-restricted cytotoxic T-lymphocyte epitope within HIV-1 Nef. (ox.ac.uk)
  • Identification of novel HLA-A2-restricted human immunodeficiency virus type 1-specific cytotoxic T-lymphocyte epitopes predicted by the HLA-A2 supertype peptide-binding motif. (ox.ac.uk)
  • The identification of virus-specific epitopes that are efficiently recognized by CTL is the first step in the development of future vaccines. (ox.ac.uk)
  • Identification of a novel human leucocyte antigen-A*01-restricted cytotoxic T-lymphocyte epitope in the respiratory syncytial virus fusion protein. (vanderbilt.edu)
  • These studies thus demonstrate the potential use of the PSA-3A agonist epitope in both peptide- and vector-mediated immunotherapy protocols for prostate cancer. (unboundmedicine.com)
  • Indeed, the entire edifice of immunotherapy with the MHC I-presented epitopes of human cancers is built on the pioneering corresponding studies in a murine system ( 12 ). (pnas.org)
  • Conclusion: These results indicate that T helper lymphocytes are capable of recognizing CEA as a tumor antigen and that epitope CEA 653-667 could be used for immunotherapy against tumors expressing CEA. (elsevier.com)
  • About 50% of cervical cancers are associated with human papillomavirus type 16 (HPV-16), and since the HPV-16 E6 and E7 oncoproteins are constitutively expressed in the tumor cells, they are attractive targets for cytotoxic T lymphocyte (CTL)-mediated immunotherapy. (fujita-hu.ac.jp)
  • Thus, these findings together indicate that E6 49-57 is a candidate epitope for immunotherapy and immunological monitoring of such patients. (fujita-hu.ac.jp)
  • Spleen cells from mice that displayed tumor regression had high percentages of CD8 + T cells that were specifically stained with L d tetramers loaded with gp70 423-431 , the antigenic epitope of gp70 protein. (aacrjournals.org)
  • The antigenic epitope is contained within the sequence EYELRK H NFSDTG and is generated by substitution of Asn by His (italic) caused by a single point mutation. (pnas.org)
  • We originally demonstrated that two virus-specific cytotoxic T-lymphocyte (CTL) epitopes, from the same virus but presented by different major histocompatibility complex alleles, when linked in tandem as minigenes in a recombinant vaccinia virus, could confer complete protection against subsequent viral challenge. (asm.org)
  • We combined several in silico prediction methods to unravel the most promising C57BL/6 mouse-restricted Hepatitis C virus (HCV) MHC class I epitopes and validated these epitopes in vitro and in vivo . (mdpi.com)
  • This study provides an up-to-date listing of currently known, well-defined viral CTL epitopes for HIV, EBV, CMV, HCV and HBV and describes an approach that overcomes some of the above limitations through the use of peptide matrices of optimally defined viral CTL epitopes in combination with anti-CD3 in vitro T cell expansion and re-use of cells from negative ELISpot wells. (harvard.edu)
  • Classical swine fever virus (CSFV) -specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. (microbiologyresearch.org)
  • We have derived HLA-A2.1-restricted, gastric cancer-specific cytotoxic T lymphocyte (CTL) lines by repetitive in vitro stimulation of tumor- associated lymphocytes (TAL) with autologous tumor cells. (elsevier.com)
  • Loss of HIV-1-derived cytotoxic T lymphocyte epitopes restricted by protective HLA-B alleles during the HIV-1 epidemic. (nih.gov)
  • Cross-sectional studies have shown an inverse correlation between the prevalence of human leukocyte antigen (HLA) alleles in a population and the number of cytotoxic T lymphocyte (CTL) escape mutations in epitopes restricted by those HLA alleles. (nih.gov)
  • The number of CTL epitopes in HIV-1 variants restricted by the most common HLA alleles in the population did not change significantly during the epidemic. (nih.gov)
  • In contrast, we found a significant loss of CTL epitopes restricted by HLA-B alleles associated with a low relative hazard of HIV-1 disease progression during the epidemic. (nih.gov)
  • Such a loss was not observed for CTL epitopes restricted by HLA-A alleles. (nih.gov)
  • Here we describe the immunological characterization of a number of novel HIV-1-specific, HLA-A2-restricted CTL epitopes that share a high degree of conservation within HIV-1 and a strong binding to different alleles of the HLA-A2 superfamily. (ox.ac.uk)
  • We define several novel non-clade B CTL epitopes, including some restricted by HLA alleles common in Africans. (ox.ac.uk)
  • HLA-B63, the serological marker for the closely related HLA-B*1516 and HLA-B*1517 alleles, shares the epitope binding motif of HLA-B57 and HLA-B58, two alleles that have been associated with slow HIV disease progression. (ox.ac.uk)
  • We have investigated the use of the protective antigen (PA) and lethal factor (LF) components of anthrax toxin as a system for in vivo delivery of cytotoxic T-lymphocyte (CTL) epitopes. (asm.org)
  • However we show that when analyzed in tissue culture and in vivo, all six epitopes are expressed. (asm.org)
  • The mutated L11 epitope is thus shown to be an immunoprotective epitope in vivo by several criteria. (pnas.org)
  • Depletion of T lymphocytes in vivo renders mice unable to be immunized ( 1 ), and adoptive transfer of T lymphocytes from tumor-immune mice can successfully transfer tumor immunity ( 2 - 9 ). (pnas.org)
  • The results show surprising and interesting similarities and differences between the two epitopes and their activities in vivo . (pnas.org)
  • Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope recognized by CSFV-specific CTL. (microbiologyresearch.org)
  • A predicted T-cell epitope, from the CT610 gene product, was presented by HLA-B27. (mcponline.org)
  • These sequences can then be used to predict where the epitope lies on the native protein, taking into account that the epitope on the native protein may be discontinuous. (proteopedia.org)
  • Sequences of the identified epitope are highly conserved among H1 and H9 subtypes. (osti.gov)
  • DI-fusion Epitopes recognized by human T lymphocytes in the ROP2. (ac.be)
  • An epitope is the portion of the surface of an antigen that binds to an antibody , or the peptide fragment of a protein antigen that binds to the T lymphocyte antigen receptor when presented by the cognate major histocompatibility protein. (proteopedia.org)
  • HIV-1 gag-specific cytotoxic T lymphocytes recognize multiple highly conserved epitopes. (jimmunol.org)
  • CTL specific for one of the epitopes were unable to recognize several naturally occurring viral variants, except at high peptide concentration, underlining the HBV subtype specificity of this response. (rupress.org)
  • CD4 + T lymphocyte clones, generated from mice immunized with the methylcholanthrene-induced fibrosarcoma Meth A (H-2 d ), are restricted by I-E d and recognize a unique antigen on Meth A. The antigen has been purified and characterized as the ribosomal protein L11. (pnas.org)
  • CD4+ T-cells from peripheral blood of a patient with psoriasis recognize keratin 14 peptide but not 'homologous' streptococcal M-protein epitope. (lsh.is)
  • HLA B8-restricted cytotoxic T lymphocytes (CTL) specific for influenza A virus were generated and shown to recognize the nucleoprotein (NP). (ox.ac.uk)
  • A further amino acid substitution at position 70 resulted in the gain of additional H-2D d specificities, allowing to localize more than half of all the relevant H-2D d serological epitopes to position 63 to 70. (springer.com)
  • An alto CTL epitope of the H-2D d antigen was also localized to this stretch of amino acid sequence, as one of several H-2D d specific CTL clones reacted with the mutant molecule in which amino acids were replaced at position 63 to 70. (springer.com)
  • The strategy involves fusing epitopes to the first 255 amino acids of LF, designated LFn. (asm.org)
  • The construct contains a single start codon, and the epitopes are linked directly, without intervening spacer amino acids. (asm.org)
  • The epitope recognized by this CD4+ CTL clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. (eurekamag.com)
  • Based on these results, it is likely that a construct expressing the 359 amino acids constituting p15 and p26 would contain epitopes capable of stimulating CTL in most horses. (microbiologyresearch.org)
  • Horwitz, MS , Yanagi, Y & Oldstone, MBA 1994, ' T-cell receptors from virus-specific cytotoxic T lymphocytes recognizing a single immunodominant nine-amino-acid viral epitope show marked diversity ', Journal of Virology , vol. 68, no. 1, pp. 352-357. (elsevier.com)
  • Two sets of criteria are available which are said to identify potential T-cell epitopes, one based on primary amino acid sequence and the second based on protein secondary structure. (scripps.edu)
  • Control of human immunodeficiency virus replication by cytotoxic T lymphocytes targeting subdominant epitopes. (washington.edu)
  • Here, we have demonstrated that endogenous processing and presentation of a human immunodominant HIV-1 epitope is more efficient than that of a subdominant epitope. (jci.org)
  • Late seroconversion in HEPS donors was associated with a switch in epitope specificity and/or immunodominance to those epitopes preferentially recognized by HIV-1-infected women. (ox.ac.uk)
  • Immunological significance of cytotoxic T lymphocyte epitope variants in patients chronically infected by the hepatitis C virus. (jci.org)
  • This study was performed to test the hypothesis that cytotoxic T lymphocyte (CTL) selection of hepatitis C virus (HCV) escape variants plays a role in HCV persistence. (jci.org)
  • Collectively, these observations suggest that CTL selection of epitope variants may have occurred in these patients before their entrance into the study and that it may have played a role in HCV persistence. (jci.org)
  • Inactivated whole-virus preparations are useful against some diseases, but these vaccines generally do not generate a potent major histocompatibility complex (MHC) class I-restricted cytotoxic T-lymphocyte (CTL) response necessary to clear lentiviral-infected cells and are not effective against lentivirus variants ( 15 , 29 ). (pubmedcentralcanada.ca)
  • Transfer of HIV-1-specific cytotoxic T lymphocytes to an AIDS patient leads to selection for mutant HIV variants and subsequent disease progression. (microbiologyresearch.org)
  • Unexpectedly, the five ligands correspond to only three cytotoxic T lymphocyte (CTL) epitopes, two of which occur in two COOH-terminal length variants. (elsevier.com)
  • During the analysis of the fine specificity of CTL clones directed to the HA 210-219 epitope, we found that one clone 40-2 also recognized the myeloma cell line P3x63-Ag8. (rupress.org)
  • Mutations responsible for this mode of escape were located within the epitope regions rather than the flanking regions, and such mutations did not influence the virus replication. (asm.org)
  • Several mutations have been described in CTL epitopes in HIV-1-infected individuals, which result in either a lack of binding to the MHC class I molecule or nonrecognition by T-cell receptor (TCR) ( 3 , 8 , 12 , 20 , 21 ). (asm.org)
  • Finally, we show that mutations in HBc141-149 epitope are associated with viral parameters and disease progression in HBV infected patients. (protein-cell.org)
  • Fingerprint Dive into the research topics of 'Simian immunodeficiency virus evades a dominant epitope-specific cytotoxic T lymphocyte response through a mutation resulting in the accelerated dissociation of viral peptide and MHC class I'. Together they form a unique fingerprint. (elsevier.com)
  • The majority of gag-specific CTL activity was found to be directed against epitopes in the p24 subunit of the gag protein, with at least seven different HLA class I-restricted CTL p24 epitopes identified in these three subjects. (jimmunol.org)
  • Less CTL activity was directed against p17 subunit of gag and two CTL epitopes were identified in this protein. (jimmunol.org)
  • We also demonstrate that BALB/c mice immunized with a single LFn fusion protein containing NP and listeriolysin O protein epitopes in tandem mount a CTL response against both pathogens. (asm.org)
  • Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein. (semanticscholar.org)
  • HLA class I-restricted cytotoxic T-cell epitopes of the respiratory syncytial virus fusion protein. (semanticscholar.org)
  • Antibody epitopes can be made up of discontinuous portions of a protein antigen's sequence, or of a continuous portion. (proteopedia.org)
  • In contrast, T cell epitopes always represent a continuous fragment of the sequence of a protein antigen. (proteopedia.org)
  • T cell epitopes are always peptide fragments, and hence, represent a denatured (unfolded) form of the native protein. (proteopedia.org)
  • ElliPro is a web-tool that implements a method for identifying continuous epitopes in the protein regions protruding from the protein's globular surface and, together with a residue clustering algorithm, the MODELLER program and the Jmol viewer, allows the prediction and visualization of antibody epitopes in a given protein sequence or structure. (proteopedia.org)
  • In addition, the CTL activation induced by the fusion protein, but not by the epitope, was inhibited by paraformaldehyde fixation of the mo-DC and by treatment with lactacystin, a specific inhibitor for the proteasome. (elsevier.com)
  • Most significantly, some of the peptide-reactive helper T lymphocytes were also capable of recognizing naturally processed antigen in the form of recombinant CEA protein or cell lysates from tumors that express CEA. (elsevier.com)
  • In this report, we describe for the first time an epitope common to human myelin basic protein (H.MBP), a structural component of central nervous system myelin and T lymphocyte CD3, an activation molecule important in signal transduction. (elsevier.com)
  • Raus, J. C.M. / A common epitope on human myelin basic protein and the human T lymphocyte CD3 molecule . (elsevier.com)
  • Multiple epitopes were recognized in a region corresponding to the major homology region of the human immunodeficiency virus, a region with significant sequence similarity to other lentiviruses including simian immunodeficiency virus, puma lentivirus, feline immunodeficiency virus, Jembrana disease virus, visna virus, and caprine arthritis encephalitis virus. (pubmedcentralcanada.ca)
  • Simian immunodeficiency virus evades a dominant epitope-specific cytotoxic T lymphocyte response through a mutation resulting in the accelerated dissociation of viral peptide and MHC class I . Journal of Immunology , 164 (12), 6474-6479. (elsevier.com)
  • In humans, indirect evidence has accumulated suggesting a similar protective role for antigen-specific CD8+ T-lymphocytes. (semanticscholar.org)
  • Epitopes described in HLA class I-restricted cytotoxic T lymphocytes specific for hepatitis C virus. (iedb.org)
  • Further, some H-2L d specific alto CTL clones lost reactivity to the mutant molecules, demonstrating the presence of CTL epitopes in this region of the H-2L d antigen. (springer.com)
  • Moreover, the antigen epitopes are strictly restricted to specific SLA molecules. (frontiersin.org)
  • Immunogenetic analysis of this apparent H-2-unrestricted killing revealed that the most plausible explanation was a sharing of an H-2-restricting epitope by H-2K S and H-2D d molecules. (elsevier.com)
  • Our data therefore provide insights into the structure characteristics of this unconventional epitope binding to MHC-I molecules, as well as epitope specific CTL activity that orchestrate T cell response and immune evasion in HBV infected patients. (protein-cell.org)
  • The reactivities of 14 of the 17 anti-GP CTL clones against the deleted GP molecules have been fully characterized, and two clear patterns of anti-GP activity have emerged, defining at least two CTL epitopes. (scripps.edu)
  • Combinatorial peptide-based epitope mapping from Ebola virus DNA vaccines and infections reveals residue-level determinants of antibody binding. (bioportfolio.com)
  • Nine previously uncharacterized CD8+ T cell epitopes were identified from HSV-2 infected BALB/c mice. (mdpi.com)
  • Furthermore, we show that NP-specific CTL are primed in both BALB/c and C57BL/6 mice when the mice are immunized with a single fusion containing two epitopes, one presented by L d and one presented by D b . (asm.org)
  • Similarly, CTL induced by the LCMV epitope protected mice against challenge with this agent. (asm.org)
  • Simian virus 40 (SV40) tumor (T) antigen expressed in H-2b SV40-transformed cells induces the generation of Lyt-2+ (CD8+) cytotoxic T lymphocytes (CTL), which are involved in tumor rejection, in syngeneic mice. (eurekamag.com)
  • Immunization of BALB/cJ mice with the mutated epitope but not with the wild-type epitope protects mice against a subsequent challenge with the Meth A sarcoma. (pnas.org)
  • When A. SW (H-2 s ) mice are immunized with B10.S (H-2 S ) epidermal cells, cytolytic T lymphocytes are evoked that efficiently lyse B10.D2 (H-2 d ) as well as B10.S target cells. (elsevier.com)
  • Epitopes recognized by the T-cell receptor are often located in the inner, unexposed side of the antigen, and become accessible to the T-cell receptors after proteolytic processing of the antigen. (citizendium.org)
  • Epitopes recognized by the B-Cell receptor are located on the surface of the Antigen . (online-medical-dictionary.org)
  • Here we show that the region of CS known to elicit a cytotoxic T-lymphocyte (CTL) response to irradiated sporozoites also, somewhat ironically, mediates the receptor-ligand interaction essential to parasite invasion of the host. (mysciencework.com)
  • Cryptic major histocompatibility complex class I epitopes have been detected in several pathogens, but their importance in the immune response to AIDS viruses remains unknown. (rupress.org)
  • The discovery of an immunodominant CD8-TL response in elite controller macaques against a cryptic epitope suggests that the AIDS virus-specific cellular immune response is likely far more complex than is generally assumed. (rupress.org)
  • Cytotoxic T lymphocytes (CTL) are essential for immune clearance of many intracellular pathogens, including most viruses and some bacteria ( 1 , 18 ). (asm.org)
  • Some of the resulting virions differ at immunologically relevant epitopes, thereby eluding existing immune mechanisms, and allowing for further dissemination throughout the host's tissue ( 7 ). (pubmedcentralcanada.ca)
  • This is a procedure for collecting quantities of lymphocytes (white blood cells that are an important part of the immune system). (clinicaltrials.gov)
  • We demonstrate for the first time in an invertebrate host-parasite interaction that a large repertoire of parasite epitopes matched a large repertoire of host immune receptors. (plos.org)
  • Moné Y, Gourbal B, Duval D, Du Pasquier L, Kieffer-Jaquinod S, Mitta G (2010) A Large Repertoire of Parasite Epitopes Matched by a Large Repertoire of Host Immune Receptors in an Invertebrate Host/Parasite Model. (plos.org)
  • This causal lentivirus can be found in monocytes, lymphocytes and macrophages of infected sheep in the presence of humoral and cell mediated immune response and can also be detected by conducting several serological tests. (wikipedia.org)
  • abstract = "Although a pivotal role of proteasomes in the proteolytic generation of epitopes for major histocompatibility complex (MHC) class I presentation is undisputed, their precise function is currently the subject of an active debate: do proteasomes generate many epitopes in definitive form, or do they merely generate the COOH termini, whereas the definitive NH2 termini are cleaved by aminopeptidases? (elsevier.com)
  • Peptide specific CD8+ human T cell lines from peripheral blood lymphocytes were generated from a HLA-A*02+ donor. (mdpi.com)
  • In this study, we describe a human monoclonal antibody (HuMAb), 5D7, that was prepared from the peripheral blood lymphocytes of a vaccinated volunteer using the fusion method. (osti.gov)
  • Tyler, JD & David, CS 1983, ' MHC epitopes of K s and D d restrict the same population of cytolytic T lymphocytes ', Immunogenetics , vol. 17, no. 5, pp. 457-464. (elsevier.com)
  • David, Chella S. / MHC epitopes of K s and D d restrict the same population of cytolytic T lymphocytes . (elsevier.com)
  • Identifying the conformational epitopes on the virus capsid supports the development of improved recombinant vaccines to maximize long-term protection against multiple types of HPV. (bioportfolio.com)
  • Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4 - CD6 + CD8 + MHC class I-restricted T lymphocytes. (microbiologyresearch.org)
  • and an antibody epitope from mengovirus. (asm.org)
  • The best way to identify an antibody epitope is from a crystal structure of the antibody:antigen complex, where the contacts are evident. (proteopedia.org)
  • Antibody epitopes may also be called determinants , which is an historically earlier but equally good term. (proteopedia.org)
  • Ponomarenko J, Bui HH, Li W, Fusseder N, Bourne PE, Sette A, Peters B. ElliPro: a new structure-based tool for the prediction of antibody epitopes. (proteopedia.org)
  • Adhesion of lymphocytes to Peyer's patch HEV, but not to peripheral node HEV, is inhibited by an antibody recognizing the murine lymphocyte antigen LPAM-1. (nih.gov)
  • By coupling intracellular staining with terminal deoxynucleotidyl transferase (TdT)-mediated labeling of internucleosomal DNA strand breaks in a flow cytometric assay, we observed a strong correlation between apoptosis- associated DNA strand breaks and immunoreactivity with the monoclonal antibody (MAb) B-F6 in activated human peripheral blood T lymphocytes (PBTs). (elsevier.com)
  • High-Resolution Structure Analysis of Antibody V5 and U4 Conformational Epitopes on Human Papillomavirus 16. (bioportfolio.com)
  • Antibody breadth or cross-reactivity is achieved by two different pathways: germline-encoded cross-reactivity for variable viral epitopes or somatic evolution in germinal centers (GCs) for conserved viral epitopes 2 . (nature.com)
  • Thus, their escape is likely due to the changes that occur during the processing and presentation of epitopes in the infected cells. (asm.org)
  • A CD4+ cytotoxic T-lymphocyte (CTL) clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. (eurekamag.com)
  • Two-parameter confocal immunofluorescence microscopy of histone and DNA staining confirmed a lack of histone immunoreactivity in viable cells and demonstrated co-localization of histone epitopes with abnormally clumped chromatin in apoptotic cells. (elsevier.com)
  • HLA DR B1*04, *07-restricted epitopes on Keratin 17 for autoreactive T cells in psoriasis. (lsh.is)
  • The lymphocytes are removed and the rest of the blood (red cells, platelets and plasma) is returned to the body through the same needle or another needle placed in the other arm. (clinicaltrials.gov)
  • The primary goal of the study is to search for candidate autoantigen(s) that drive the autoimmune response and identify candidate pathogenic T-cell epitopes among the endomysial T-cells extracted from the patients' muscle biopsies. (clinicaltrials.gov)
  • Using reverse immunological methods, we have generated a CTL clone against the HPV-16 E6 49-57 epitope restricted by HLA-A*2402, which is the most commun allele in Japan and relatively frequent worldwide, capable of lysing 293T cells transduced with HLA-A*2402 and HPV-16 E6. (fujita-hu.ac.jp)
  • The kit is optimized for high yield of tumor cells and tumor-infiltrating lymphocytes. (miltenyibiotec.com)
  • The kit has been designed to obtain tumor cells and tumor-infiltrating lymphocytes, while preserving cell surface epitopes. (miltenyibiotec.com)