UDPglucose 4-Epimerase: A necessary enzyme in the metabolism of galactose. It reversibly catalyzes the conversion of UDPglucose to UDPgalactose. NAD+ is an essential component for enzymatic activity. EC 5.1.3.2.Carbohydrate Epimerases: Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3.Racemases and Epimerases: Enzymes that catalyze inversion of the configuration around an asymmetric carbon in a substrate having one (racemase) or more (epimerase) center(s) of asymmetry. (Dorland, 28th ed) EC 5.1.Iduronic Acid: Component of dermatan sulfate. Differs in configuration from glucuronic acid only at the C-5 position.Isomerases: A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.Uridine Diphosphate Galactose: A nucleoside diphosphate sugar which can be epimerized into UDPglucose for entry into the mainstream of carbohydrate metabolism. Serves as a source of galactose in the synthesis of lipopolysaccharides, cerebrosides, and lactose.Uridine Diphosphate SugarsAmino Acid Isomerases: Enzymes that catalyze either the racemization or epimerization of chiral centers within amino acids or derivatives. EC 5.1.1.N-Acetylneuraminic Acid: An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)Galactose: An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.Galactosemias: A group of inherited enzyme deficiencies which feature elevations of GALACTOSE in the blood. This condition may be associated with deficiencies of GALACTOKINASE; UDPGLUCOSE-HEXOSE-1-PHOSPHATE URIDYLYLTRANSFERASE; or UDPGLUCOSE 4-EPIMERASE. The classic form is caused by UDPglucose-Hexose-1-Phosphate Uridylyltransferase deficiency, and presents in infancy with FAILURE TO THRIVE; VOMITING; and INTRACRANIAL HYPERTENSION. Affected individuals also may develop MENTAL RETARDATION; JAUNDICE; hepatosplenomegaly; ovarian failure (PRIMARY OVARIAN INSUFFICIENCY); and cataracts. (From Menkes, Textbook of Child Neurology, 5th ed, pp61-3)Uridine Diphosphate Glucose: A key intermediate in carbohydrate metabolism. Serves as a precursor of glycogen, can be metabolized into UDPgalactose and UDPglucuronic acid which can then be incorporated into polysaccharides as galactose and glucuronic acid. Also serves as a precursor of sucrose lipopolysaccharides, and glycosphingolipids.Hexuronic Acids: Term used to designate tetrahydroxy aldehydic acids obtained by oxidation of hexose sugars, i.e. glucuronic acid, galacturonic acid, etc. Historically, the name hexuronic acid was originally given to ascorbic acid.HexosaminesGlucuronic Acid: A sugar acid formed by the oxidation of the C-6 carbon of GLUCOSE. In addition to being a key intermediate metabolite of the uronic acid pathway, glucuronic acid also plays a role in the detoxification of certain drugs and toxins by conjugating with them to form GLUCURONIDES.Uridine Diphosphate Xylose: The decarboxylation product of UDPglucuronic acid, which is used for formation of the xylosides of seryl hydroxyl groups in mucoprotein synthesis. Also forms plant xylans.Enoyl-CoA Hydratase: An enzyme that catalyzes reversibly the hydration of unsaturated fatty acyl-CoA to yield beta-hydroxyacyl-CoA. It plays a role in the oxidation of fatty acids and in mitochondrial fatty acid synthesis, has broad specificity, and is most active with crotonyl-CoA. EC 4.2.1.17.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Uridine Diphosphate N-Acetylglucosamine: Serves as the biological precursor of insect chitin, of muramic acid in bacterial cell walls, and of sialic acids in mammalian glycoproteins.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Galactosephosphates: Phosphoric acid esters of galactose.Dodecenoyl-CoA Isomerase: A carbon-carbon double bond isomerase that catalyzes the movement double bond from C3 to C2 of an unsaturated acyl-CoA. The enzyme plays a key role in allowing acyl-CoA substrates to re-enter the beta-oxidation pathway.UTP-Hexose-1-Phosphate Uridylyltransferase: An enzyme that catalyzes the synthesis of UDPgalactose from UTP and galactose-1-phosphate. It is present in low levels in fetal and infant liver, but increases with age, thereby enabling galactosemic infants who survive to develop the capacity to metabolize galactose. EC 2.7.7.10.ArabinoseUracil NucleotidesGuanosine Diphosphate Sugars: Esters formed between the aldehydic carbon of sugars and the terminal phosphate of guanosine diphosphate.Azotobacter vinelandii: A species of gram-negative, aerobic bacteria first isolated from soil in Vineland, New Jersey. Ammonium and nitrate are used as nitrogen sources by this bacterium. It is distinguished from other members of its genus by the ability to use rhamnose as a carbon source. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Sialic Acid Storage Disease: Autosomal recessive neurodegenerative disorders caused by lysosomal membrane transport defects that result in accumulation of free sialic acid (N-ACETYLNEURAMINIC ACID) within the lysosomes. The two main clinical phenotypes, which are allelic variants of the SLC17A5 gene, are ISSD, a severe infantile form, or Salla disease, a slowly progressive adult form, named for the geographic area in Finland where the kindred first studied resided.Alginates: Salts of alginic acid that are extracted from marine kelp and used to make dental impressions and as absorbent material for surgical dressings.HexosesDistal Myopathies: A heterogeneous group of genetic disorders characterized by progressive MUSCULAR ATROPHY and MUSCLE WEAKNESS beginning in the hands, the legs, or the feet. Most are adult-onset autosomal dominant forms. Others are autosomal recessive.Uridine Diphosphate N-Acetylgalactosamine: A nucleoside diphosphate sugar which serves as a source of N-acetylgalactosamine for glycoproteins, sulfatides and cerebrosides.Dermatan Sulfate: A naturally occurring glycosaminoglycan found mostly in the skin and in connective tissue. It differs from CHONDROITIN SULFATE A (see CHONDROITIN SULFATES) by containing IDURONIC ACID in place of glucuronic acid, its epimer, at carbon atom 5. (from Merck, 12th ed)Disaccharides: Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.Diaminopimelic AcidGuanosine Diphosphate Fucose: A nucleoside diphosphate sugar formed from GDPmannose, which provides fucose for lipopolysaccharides of bacterial cell walls, and for blood group substances and other glycoproteins.UTP-Glucose-1-Phosphate Uridylyltransferase: An enzyme that catalyzes the formation of UDPglucose from UTP plus glucose 1-phosphate. EC 2.7.7.9.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.NAD: A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Rhodothermus: A genus of obligately aerobic, thermophilic, gram-negative bacteria in the family Crenotrichaceae. They were isolated from submarine alkaline HOT SPRINGS in Iceland.Ruminococcus: A genus of gram-positive bacteria in the family Lachnospiraceae that inhabits the RUMEN; LARGE INTESTINE; and CECUM of MAMMALS.Kinetics: The rate dynamics in chemical or physical systems.Nucleoside Diphosphate SugarsPropionibacterium: A genus of gram-positive, rod-shaped bacteria whose cells occur singly, in pairs or short chains, in V or Y configurations, or in clumps resembling letters of the Chinese alphabet. Its organisms are found in cheese and dairy products as well as on human skin and can occasionally cause soft tissue infections.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Amino Acids, DiaminoRhamnose: A methylpentose whose L- isomer is found naturally in many plant glycosides and some gram-negative bacterial lipopolysaccharides.Uridine Diphosphate Glucuronic Acid: A nucleoside diphosphate sugar which serves as a source of glucuronic acid for polysaccharide biosynthesis. It may also be epimerized to UDP iduronic acid, which donates iduronic acid to polysaccharides. In animals, UDP glucuronic acid is used for formation of many glucosiduronides with various aglycones.Kluyveromyces: An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.Galactokinase: An enzyme that catalyzes reversibly the formation of galactose 1-phosphate and ADP from ATP and D-galactose. Galactosamine can also act as the acceptor. A deficiency of this enzyme results in GALACTOSEMIA. EC 2.7.1.6.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Cellobiose: A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.3-Hydroxyacyl CoA Dehydrogenases: Enzymes that reversibly catalyze the oxidation of a 3-hydroxyacyl CoA to 3-ketoacyl CoA in the presence of NAD. They are key enzymes in the oxidation of fatty acids and in mitochondrial fatty acid synthesis.Pentoses: A class of carbohydrates that contains five carbon atoms.PentosephosphatesSaccharomyces: A genus of ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES.Carbon-Carbon Double Bond Isomerases: Enzymes that catalyze the shifting of a carbon-carbon double bond from one position to another within the same molecule. EC 5.3.3.Heparitin Sulfate: A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.Multienzyme Complexes: Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.Myositis, Inclusion Body: Progressive myopathies characterized by the presence of inclusion bodies on muscle biopsy. Sporadic and hereditary forms have been described. The sporadic form is an acquired, adult-onset inflammatory vacuolar myopathy affecting proximal and distal muscles. Familial forms usually begin in childhood and lack inflammatory changes. Both forms feature intracytoplasmic and intranuclear inclusions in muscle tissue. (Adams et al., Principles of Neurology, 6th ed, pp1409-10)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Thulium: Thulium. An element of the rare earth family of metals. It has the atomic symbol Tm, atomic number 69, and atomic weight 168.93.Phosphotransferases (Alcohol Group Acceptor): A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.Sulfotransferases: Enzymes which transfer sulfate groups to various acceptor molecules. They are involved in posttranslational sulfation of proteins and sulfate conjugation of exogenous chemicals and bile acids. EC 2.8.2.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Cytidine Monophosphate N-Acetylneuraminic Acid: A nucleoside monophosphate sugar which donates N-acetylneuraminic acid to the terminal sugar of a ganglioside or glycoprotein.Methylmalonyl-CoA Mutase: An enzyme that catalyzes the conversion of methylmalonyl-CoA to succinyl-CoA by transfer of the carbonyl group. It requires a cobamide coenzyme. A block in this enzymatic conversion leads to the metabolic disease, methylmalonic aciduria. EC 5.4.99.2.Uridine Diphosphate: A uracil nucleotide containing a pyrophosphate group esterified to C5 of the sugar moiety.Carbohydrate Dehydrogenases: Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.Ralstonia: A genus in the family BURKHOLDERIACEAE, comprised of many species. They are associated with a variety of infections including MENINGITIS; PERITONITIS; and URINARY TRACT INFECTIONS.Catalytic Domain: The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Ribulosephosphates: Ribulose substituted by one or more phosphoric acid moieties.Mast-Cell Sarcoma: A unifocal malignant tumor that consists of atypical pathological MAST CELLS without systemic involvement. It causes local destructive growth in organs other than in skin or bone marrow.Xylulose: A 5-carbon keto sugar.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Genes, Bacterial: The functional hereditary units of BACTERIA.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Ribitol: A sugar alcohol formed by the reduction of ribose.Transketolase: An enzyme of the transferase class that catalyzes the conversion of sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to D-ribose 5-phosphate and D-xylulose 5-phosphate in the PENTOSE PHOSPHATE PATHWAY. (Dorland, 27th ed) EC 2.2.1.1.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.HeptosesBacterial Proteins: Proteins found in any species of bacterium.Biocatalysis: The facilitation of biochemical reactions with the aid of naturally occurring catalysts such as ENZYMES.Uridine Monophosphate: 5'-Uridylic acid. A uracil nucleotide containing one phosphate group esterified to the sugar moiety in the 2', 3' or 5' position.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Pentose Phosphate Pathway: An oxidative decarboxylation process that converts GLUCOSE-6-PHOSPHATE to D-ribose-5-phosphate via 6-phosphogluconate. The pentose product is used in the biosynthesis of NUCLEIC ACIDS. The generated energy is stored in the form of NADPH. This pathway is prominent in tissues which are active in the synthesis of FATTY ACIDS and STEROIDS.Aeromonas hydrophila: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that may be pathogenic for frogs, fish, and mammals, including man. In humans, cellulitis and diarrhea can result from infection with this organism.Bromosuccinimide: A brominating agent that replaces hydrogen atoms in benzylic or allylic positions. It is used in the oxidation of secondary alcohols to ketones and in controlled low-energy brominations. (From Miall's Dictionary of Chemistry, 5th ed; Hawley's Condensed Chemical Dictionary, 12th ed,).Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.PolysaccharidesAdenosine Diphosphate Sugars: Esters formed between the aldehydic carbon of sugars and the terminal phosphate of adenosine diphosphate.Sialic Acids: A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.Phosphoglucomutase: An enzyme that catalyzes the conversion of alpha D-glucose 1-phosphate to alpha D-glucose 6-phosphate. EC 5.4.2.2.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.UDPglucose-Hexose-1-Phosphate Uridylyltransferase: An enzyme that catalyzes the transfer of UMP from UDPglucose to galactose 1-phosphate, forming UDPgalactose and glucose 1-phosphate. Deficiency in this enzyme is the major cause of GALACTOSEMIA. EC 2.7.7.12.Chromatography, Paper: An analytical technique for resolution of a chemical mixture into its component compounds. Compounds are separated on an adsorbent paper (stationary phase) by their varied degree of solubility/mobility in the eluting solvent (mobile phase).Hydro-Lyases: Enzymes that catalyze the breakage of a carbon-oxygen bond leading to unsaturated products via the removal of water. EC 4.2.1.Models, Chemical: Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.Carbohydrate Metabolism, Inborn ErrorsMagnetic Resonance Spectroscopy: Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Intramolecular Transferases: Enzymes of the isomerase class that catalyze the transfer of acyl-, phospho-, amino- or other groups from one position within a molecule to another. EC 5.4.Sequence Analysis: A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information.Isomerism: The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Electrophoresis, Paper: Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used.Polyisoprenyl Phosphate Monosaccharides: These compounds function as activated monosaccharide carriers in the biosynthesis of glycoproteins and oligosaccharide phospholipids. Obtained from a nucleoside diphosphate sugar and a polyisoprenyl phosphate.
MMAB Methylmalonyl-CoA epimerase deficiency; 251120; MCEE Mevalonic aciduria; 610377; MVK MHC class II deficiency, ... GALK1 Galactose epimerase deficiency; 230350; GALE Galactosemia; 230400; GALT Galactosialidosis; 256540; CTSA Gallbladder ...
UDP galactose epimerase. galactose epimerase deficiency, UDP-Galactose-4-epimerase deficiency Normal metabolic pathway for ...
Methylmalonyl CoA epimerase. References[edit]. *^ Tabita, F. R., "The hydroxypropionate pathway of CO2 fixation: Fait accompli ...
Dermatan-sulfate epimerase is an enzyme that in humans is encoded by the DSE gene. The protein encoded by this gene is a tumor- ... "Entrez Gene: DSE dermatan sulfate epimerase". Sasatomi T, Suefuji Y, Matsunaga K, Yamana H, Miyagi Y, Araki Y, Ogata Y, Itoh K ... chondroitin-glucuronate C5-epimerase is identical to SART2". J Biol Chem. 281 (17): 11560-8. doi:10.1074/jbc.M513373200. PMID ... chondroitin-glucuronate C5-epimerase is identical to SART2". J. Biol. Chem. 281 (17): 11560-8. doi:10.1074/jbc.M513373200. PMID ...
Galactose UDP galactose epimerase Uridine diphosphate. ...
D-glucuronyl C5-epimerase is an enzyme that in humans is encoded by the GLCE gene. GRCh38: Ensembl release 89: ENSG00000138604 ... 2008). "Decreased expression of human D-glucuronyl C5-epimerase in breast cancer". Int. J. Cancer. 122 (5): 1172-6. doi:10.1002 ... 2001). "Characterization of the D-glucuronyl C5-epimerase involved in the biosynthesis of heparin and heparan sulfate". J. Biol ... "Entrez Gene: GLCE glucuronic acid epimerase". Grigorieva E, Eshchenko T, Rykova VI, et al. ( ...
... produces Cellobiose 2-Epimerase. Parte, A.C. "Cellulosilyticum". www.bacterio.net. " ...
Chang S, Duerr B, Serif G (1988). "An epimerase-reductase in L-fucose synthesis". J. Biol. Chem. 263 (4): 1693-7. PMID 3338988 ... 5-epimerase-4-reductase. This enzyme participates in fructose and mannose metabolism. ...
The phenotypic differences caused by a deficiency of the epimerase as opposed to the mutase are so mild that there is debate ... Like the mutase, the epimerase also functions in breaking down the same substances, but to a significantly lesser extent than ... Mutations in the MCEE gene, which encodes the methylmalonyl CoA epimerase protein, also referred to as methylmalonyl racemase, ... methylmalonyl CoA epimerase, or those involved in adenosylcobalamin synthesis. It is estimated that as many as 60% of cases are ...
Ribulose-phosphate 3-epimerase is an enzyme that in humans is encoded by the RPE gene. GRCh38: Ensembl release 89: ... q34 in a child with half normal activity of ribulose 5-phosphate 3-epimerase (RPE)". J. Med. Genet. 25 (12): 850-1. doi:10.1136 ... and ribulose 5-phosphate 3-epimerase (RPE)". Ann. Hum. Genet. 43 (4): 335-42. doi:10.1111/j.1469-1809.1980.tb01567.x. PMID ... gene dosage effect of ribulose 5-phosphate 3-epimerase". Hum. Genet. 79 (1): 92. doi:10.1007/BF00291721. PMID 3366467. Spencer ...
Xu5P is converted into RuP by phosphopentose epimerase. Finally, phosphoribulokinase (another plant-unique enzyme of the ...
Dickensen F.; Williamson D. H. (1956). "Pentose phosphate isomerase and epimerase from animal tissues". Biochem. J. 64 (3): 567 ... RPIA converts Ru5P to R5P which then is converted by ribulose-phosphate 3-epimerase to xylulose-5-phosphate (figure 3). The end ...
Epimerisation is catalysed by one enzyme, the GlcA C5 epimerase or heparosan-N-sulfate-glucuronate 5-epimerase (EC 5.1.3.17). ... 2-O-sulfotransferase and C5-epimerases". Developmental Dynamics. 236 (2): 581-6. doi:10.1002/dvdy.21051. PMID 17195182. Xu, D; ... These enzymes consist of multiple glycosyltransferases, sulfotransferases and an epimerase. These same enzymes also synthesize ... it undergoes a series of modification reactions carried out by four classes of sulfotransferases and an epimerase. The ...
Category:EC 5.1 (racemases and epimerases)Edit. *Category:EC 5.1.1. *Amino-acid racemase: Phenylalanine racemase (ATP- ...
In the rate-limiting step of the pathway, UDP-GlcNAc is converted into ManNAc by UDP-GlcNAc 2-epimerase, encoded by the ... The UDP-GlcNAc 2-epimerase kinase is the rate limiting step in sialic acid biosynthesis. If the enzyme does not work ... Keppler, O; Hinderlich, S; Langner, J; Schwartz-Albiez, R; Reutter, W; Pawlita, M (1999). "UDP-GlcNAc 2-epimerase: a regulator ... When the GNE epimerase kinase does not function correctly in the human body thereby reducing the available ManNAc, it is ...
... (aldose 1-epimerase) (gene name GALM) is a human enzyme that converts alpha-aldose to the beta-anomer. It ... belongs to family of aldose epimerases. GRCh38: Ensembl release 89: ENSG00000143891 - Ensembl, May 2017 GRCm38: Ensembl release ...
Monder C, Martin KO, Bogumil J (1980). "Presence of epimerase activity in hamster liver corticosteroid side chain isomerase". J ...
The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The ... Huang XF, Luu-The V (2000). "Molecular characterization of a first human 3(alpha-->beta)-hydroxysteroid epimerase". J. Biol. ... hydroxysteroid epimerase". Biochim. Biophys. Acta. 1520 (2): 124-30. doi:10.1016/s0167-4781(01)00247-0. PMID 11513953. ... oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone ...
The UDP-glucose 4-epimerase Uge3 is responsible for these conversions. Bardalaye, P.C., and Nordin, J.H. (1976). ... Overlapping and distinct roles of Aspergillus fumigatus UDP-glucose 4-epimerases in galactose metabolism and the synthesis of ...
1994). "Purification and characterization of D-tagatose 3-epimerase from Pseudomonas sp. ST-24". Biosci Biotechnol Biochem. 58 ... D-tagatose 3-epimerase, to convert fructose to D-psicose in 1994. This method of production has a high yield, but suffers from ... "Preparation of D-psicose from D-fructose by immobilized D-tagatose 3-epimerase". J Fermentation and Bioengineering. Elsevier B. ...
tsuyamenensis as a metabolic product catalyzed by the enzyme nocardicin-A epimerase.. ...
The bifunctional enzyme, UDP-N-acetylglucosamine 2-epimerase (UDP-GlcNAc 2-epimerase/N-acetylmannosamine kinase) regulates and ... UDP-GlcNAc 2-epimerase activity is rate-limiting for the biosynthesis of sialic acid and is required for sialylation in ... Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase is an enzyme that in humans is encoded by the GNE ... Seppala R, Lehto VP, Gahl WA (1999). "Mutations in the human UDP-N-acetylglucosamine 2-epimerase gene define the disease ...
N-acylglucosamine 2-epimerase is an enzyme that in humans is encoded by the RENBP gene. The gene product inhibits renin ... Luchansky SJ, Yarema KJ, Takahashi S, Bertozzi CR (2003). "GlcNAc 2-epimerase can serve a catabolic role in sialic acid ... 1999). "Human renin-binding protein is the enzyme N-acetyl-D-glucosamine 2-epimerase". J. Biochem. 125 (2): 348-53. doi:10.1093 ... Molecular cloning and functional expression of UDP-N-acetyl-glucosamine 2-epimerase/N-acetylmannosamine kinase". J. Biol. Chem ...
The systematic name of this enzyme class is 2-methylacyl-CoA 2-epimerase. In vitro experiments with the human enzyme AMACR 1A ... This enzyme belongs to the family of isomerases, specifically the racemases and epimerases which act on other compounds. ...
This is subsequently converted to (R)-methylmalonyl-CoA by mitochondrial methylmalonyl-CoA epimerase. Finally, mitochondrial ...
DAP epimerase catalyzes the conversion of L,L-diaminopimelate to the meso form of L,L-diaminopimelate. DAP decarboxylase ... Diaminopimelate epimerase catalyzes the inversion of the α-carbon of L,L-diaminopimelic acid to yield meso-diaminopimelic acid ... "Dynamics of catalysis revealed from the crystal structures of mutants of diaminopimelate epimerase". Biochemical and ...
... encoding the key enzyme in sialic acid synthesis UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase: a missense ...
The systematic name of this enzyme class is maltose 1-epimerase. Shirokane Y, Suzuki M (1995). "A novel enzyme, maltose 1- ... In enzymology, a maltose epimerase (EC 5.1.3.21) is an enzyme that catalyzes the chemical reaction alpha-maltose ⇌ {\ ... This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and ... epimerase from Lactobacillus brevis IFO 3345". FEBS Lett. 367 (2): 177-9. doi:10.1016/0014-5793(95)00524-D. PMID 7796915. ...
The systematic name of this enzyme class is cellobiose 2-epimerase. Enzymes like these can produce a more rapid syndrome that ... This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and their ... In enzymology a cellobiose epimerase (EC 5.1.3.11) is an enzyme that catalyzes the chemical reaction cellobiose ⇌ {\ ...
Definition of aldose 1-epimerase. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and ... aldose 1-epimerase. Pronunciation: al′dōs ep′i-mĕr-ās. Definition: An enzyme catalyzing the reversible interconversion of α- ...
Putative N-acetylmannosamine-6-phosphate epimerase (IPR007260). Short name: NanE Overlapping homologous superfamilies *Ribulose ... This family represents a putative epimerase that converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6 ... Cloning, sequence, and transcriptional regulation of the operon encoding a putative N-acetylmannosamine-6-phosphate epimerase ( ...
UDP-N-acetylglucosamine 2-epimerase domain (IPR003331). Short name: UDP_GlcNAc_Epimerase_2_dom ... This entry represents a domain found in the bacterial UDP-N-acetylglucosamine 2-epimerase WecB, which is involved in the ... Proteins containing this domain also include UDP-N,N-diacetylbacillosamine 2-epimerase, which is involved in biosynthesis of ... which has both the UDP-N-acetylglucosamine 2-epimerase and the N-acetylmannosamine kinase functions. GNE catalyses the first ...
Gene Ontology Term: NADHX epimerase activity. GO ID. GO:0052856 Aspect. Molecular Function. Description. Catalysis of the ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
RPEP1 ribulose-5-phosphate-3-epimerase pseudogene 1 [Homo sapiens] RPEP1 ribulose-5-phosphate-3-epimerase pseudogene 1 [Homo ... ribulose-5-phosphate-3-epimerase pseudogene 1provided by HGNC. Primary source. HGNC:HGNC:44520 Gene type. pseudo. RefSeq status ... RPEP1 ribulose-5-phosphate-3-epimerase pseudogene 1 [ Homo sapiens (human) ] Gene ID: 100420512, updated on 12-Oct-2019 ...
Rabbit polyclonal Glucuronic Acid Epimerase antibody. Validated in IP and tested in Human. Immunogen corresponding to full ... Anti-Glucuronic Acid Epimerase antibody. See all Glucuronic Acid Epimerase primary antibodies. ... Lane 1 Primary: Glucuronic Acid Epimerase mouse polyclonal antibody. Sample: Glucuronic Acid Epimerase transfected lysate ... Glucuronic Acid Epimerase (GLCE) is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of ...
Diaminopimelate epimerase (dapF). This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself ... DAP epimeraseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB automatic annotation ... Diaminopimelate epimeraseUniRule annotation. ,p>Manual validated information which has been generated by the UniProtKB ... Belongs to the diaminopimelate epimerase family.UniRule annotation. ,p>Manual validated information which has been generated by ...
Putative epimeraseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a ... EpimeraseInterPro annotation. ,p>Information which has been generated by the UniProtKB automatic annotation system, without ... Predicted epimerase, with NAD(P)-binding Rossmann-fold domain. Escherichia coli O55:H7 ... Putative epimerase, with NAD(P)-binding Rossmann-fold domain. Escherichia coli D6-113.11 ...
Rabbit polyclonal Glucuronic Acid Epimerase/GLCE antibody. Validated in IP and tested in Human. Immunogen corresponding to full ... Anti-Glucuronic Acid Epimerase/GLCE antibody. See all Glucuronic Acid Epimerase/GLCE primary antibodies. ... Lane 1 Primary: Glucuronic Acid Epimerase/GLCE mouse polyclonal antibody. Sample: Glucuronic Acid Epimerase/GLCE transfected ... Primary - Rabbit Anti-Glucuronic Acid Epimerase/GLCE antibody (ab169024) IP Secondary - Goat Anti-Rabbit IgG H&L (HRP) ( ...
d-glucuronyl C5-epimerase (GLCE) is involved in breast and lung carcinogenesis as a potential tumor suppressor gene, acting ... Decreased expression of human d-glucuronyl C5-epimerase in breast cancer. Int J Cancer. 2008;122:1172-6.PubMedCrossRefGoogle ... Antiproliferative effect of d-glucuronyl C5-epimerase in human breast cancer cells. Cancer Cell Int. 2010;10:27.PubMedCentral ... Raedts J, Lundgren M, Kengen SW, Li JP, van der Oost J. A novel bacterial enzyme with d-glucuronyl C5-epimerase activity. J ...
Antibodies for proteins involved in diaminopimelate epimerase activity pathways, according to their Panther/Gene Ontology ...
methylmalonyl-CoA epimerase , methylmalonyl CoA epimerase , methylmalonyl-CoA epimerase, mitochondrial , DL-methylmalonyl-CoA ... anti-Methylmalonyl CoA Epimerase (MCEE) Antikörper. Bezeichnung:. anti-Methylmalonyl CoA Epimerase Antikörper (MCEE). Auf www. ... Alternative names and synonyms associated with Methylmalonyl CoA Epimerase (MCEE) * methylmalonyl-CoA epimerase (MCEE) ... Weitere Antikörper gegen Methylmalonyl CoA Epimerase Interaktionspartner. Human Methylmalonyl CoA Epimerase (MCEE) ...
Antibodies for proteins involved in N-acylglucosamine 2-epimerase activity pathways, according to their Panther/Gene Ontology ... Antibodies for proteins involved in N-acylglucosamine 2-epimerase activity pathways; according to their Panther/Gene Ontology ...
Aldose-1-epimerase (Galactose mutarotase) (galM) Antikörper * galactose mutarotase (aldose 1-epimerase) L homeolog (galm.L) ... galactose mutarotase (aldose 1-epimerase) , galactose mutarotase , Aldose-1-epimerase (Galactose mutarotase) , aldose 1- ... Galactose Mutarotase (Aldose 1-Epimerase) (GALM) Antigen-Profil Protein Überblick This gene encodes an enzyme that catalyzes ... Top anti-Galactose Mutarotase (Aldose 1-Epimerase) Antikörper auf antikoerper-online.de. Showing 10 out of 70 products:. ...
Author Summary Tuberculosis is still a major threat to global health. The disease in humans is caused by a bacterium, Mycobacterium tuberculosis, and treatment of an infected individual requires more than six months of chemotherapy. Because such a long course of treatment is required, compliance is low, which can result in the development of multidrug resistant strains (MDR-TB) and even extremely resistant strains (XDR-TB). Identifying new drug targets and potential lead therapeutic compounds are needed to combat MDR-XDR-TB. We developed a new type of assay based on the visualization of mycobacterium replication within host cells and applied it for the search of compounds that are able to chase the pathogen from its hideout. As a result, we found 20 new series of drug candidates that are effective against the bacilli in its hiding place, potentially addressing a crucial aspect in the resilience of the disease. We also showed that one series of compounds acts by inhibiting a key enzyme required for the
Exploring decaprenyl-phosphoryl ribose epimerase (DprE1) as a validated target for TB drug discovery: Assay development, high- ... Exploring decaprenyl-phosphoryl ribose epimerase (DprE1) as a validated target for TB drug discovery: Assay development, high- ... Final Report Summary - DPRETB (Exploring decaprenyl-phosphoryl ribose epimerase (DprE1) as a validated target for TB drug ... namely decaprenyl-phosphoryl ribose epimerase (DprE1), an enzyme that in conjunction with DprE2 catalyses the transformation of ...
Carbohydrate Epimerases: Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. ... Carbohydrate Epimerases. Subscribe to New Research on Carbohydrate Epimerases Enzymes that catalyze the epimerization of chiral ... Carbohydrate Epimerases*UDP acetylglucosamine-2-epimerase: 14. *UDPglucose 4-Epimerase: 8. *N-acyl-D-glucosamine 2-epimerase: 4 ...
What is aldose 1-epimerase? Meaning of aldose 1-epimerase medical term. What does aldose 1-epimerase mean? ... Looking for online definition of aldose 1-epimerase in the Medical Dictionary? aldose 1-epimerase explanation free. ... aldose 1-epimerase. al·dose 1-e·pim·er·ase. (aldōs epi-mĕr-ās), An enzyme catalyzing the reversible interconversion of α- and ... Aldose 1-epimerase , definition of aldose 1-epimerase by Medical dictionary https://medical-dictionary.thefreedictionary.com/ ...
... Li, Jin-Ping Uppsala University, Disciplinary Domain of ...
Uridine diphosphoglucose 4-epimerase information including symptoms, causes, diseases, symptoms, treatments, and other medical ... Description of Uridine diphosphoglucose 4-epimerase. Uridine diphosphoglucose 4-epimerase: Related Topics. These medical ... Epimerase *SYN *UDPglucose Interesting Medical Articles:. *Symptoms of the Silent Killer Diseases *Online Diagnosis *Self ... condition or symptom topics may be relevant to medical information for Uridine diphosphoglucose 4-epimerase: *Uridine * ...
"Finnish, Swedish Inventors Develop Glucuronyl C5-Epimerase." US Fed News Service, Including US State News. 2008. HighBeam ... The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion ... "Finnish, Swedish Inventors Develop Glucuronyl C5-Epimerase." US Fed News Service, Including US State News. The Associated ... "Finnish, Swedish Inventors Develop Glucuronyl C5-Epimerase." US Fed News Service, Including US State News. The Associated ...
  • Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria. (antikoerper-online.de)
  • Bikker H, Bakker HD, Abeling NG, Poll-The BT, Kleijer WJ, Rosenblatt DS, Waterham HR, Wanders RJ, Duran M: A homozygous nonsense mutation in the methylmalonyl-CoA epimerase gene (MCEE) results in mild methylmalonic aciduria. (hmdb.ca)
  • Sequence analysis of the gene disrupted by the transposon revealed that it matched a nucleotide-sugar epimerase of Vibrio cholerae O139, with 75 and 85% identities at the nucleotide and amino acid levels, respectively. (asm.org)
  • DNA sequence of the cloned chromosomal region surrounding one transposon insertion revealed that the transposon had disrupted an open reading frame (ORF) that was found highly similar to the nucleotide-sugar epimerase gene of Vibrio cholerae O139. (asm.org)
  • Galactose epimerase deficiency is caused by mutations in the GALE gene (1p36) encoding the UDP-galactose 4-epimerase enzyme . (nih.gov)
  • An important gene associated with Generalized Galactose Epimerase Deficiency is GALE (UDP-Galactose-4-Epimerase). (malacards.org)
  • Bonin CP, Reiter WD (2000) A bifunctional epimerase-reductase acts downstream of the MUR1 gene product and completes the de novo synthesis of GDP-L-fucose in Arabidopsis. (springer.com)
  • A new epimerase gene from Pseudomonas syringae pv glycinea PG4180, which partially showed high sequence similarity with the A. vinelandii algE-epimerases, was characterized and found to encode a bifunctional enzyme. (bibsys.no)
  • In contrast to bacteria and yeast, expression of the UDP‐Glc epimerase gene in Arabidopsis was found not to be induced by galactose. (deepdyve.com)
  • The ability of D-glucuronyl С5-epimerase to suppress proliferation of breast cancer cells MCF7 through the attenuated expression of different key genes involved in cell cycle regulation, angiogenesis and metastasis molecular pathways supports the idea on the involvement of the gene in regulation of breast cancer cell proliferation. (biomedcentral.com)
  • Provided are a protein having an amino acid sequence of SEQ ID NO:1 and having a psicose 3-epimerase activity, a gene encoding the protein, a recombinant expression vector containing the gene, and a method of producing D-psicose by reacting the protein produced on a mass scale with D-fructose. (patentsencyclopedia.com)
  • GO annotations related to this gene include hydrolase activity, hydrolyzing O-glycosyl compounds and UDP-N-acetylglucosamine 2-epimerase activity . (genecards.org)
  • The causative gene, GNE, codes for the bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) that catalyzes the first two steps in the biosynthesis of sialic acid (SA). (clinicaltrials.gov)
  • Pseudomonas cichorii D-tagatose 3-epimerase (PcDTE), which has a broad substrate specificity, efficiently catalyzes the epimerization of not only D-tagatose to D-sorbose but also D-fructose to D-psicose (D-allulose) and also recognizes the deoxy sugars as substrates. (springer.com)
  • Together with cefD2, catalyzes the reversible isomerization between isopenicillin N and penicillin N. This two-component IPN epimerase system may function by two sequential steps, an activation of isopenicillin N by the acyl-CoA synthase component cefD1, followed by epimerization by the acyl-CoA racemase component cefD2. (mybiosource.com)
  • Here, we describe a detailed method for measuring the total activity of HS C5-epimerase that involves the following steps: H/D exchange upon epimerization of the substrate with HS C5-epimerase, low-pH nitrous acid treatment of the substrate, the separation of low-pH nitrous acid-cleaved disaccharides using HPLC, and mass spectrometry analysis. (ccamp.res.in)
  • UlaD catalyzes a β-keto acid decarboxylation reaction to produce l-xylulose-5-phosphate, which undergoes successive epimerization reactions with UlaE (l-xylulose-5-phosphate 3-epimerase) and UlaF (l-ribulose-5-phosphate 4-epimerase), yielding d-xylulose-5-phosphate, an intermediate in the pentose phosphate pathway. (gc.ca)
  • Based on computational docking of the l-xylulose 5-phosphate substrate to UlaE and structural similarities of the active site of this enzyme to the active sites of other epimerases, a metal-dependent epimerization mechanism for UlaE is proposed, and Glu155 and Glu251 are implicated as catalytic residues. (gc.ca)
  • Further, it was demonstrated that the activity of the precursor-producing enzyme UDP-N-acetylglucosamine 4-epimerase, converting UDP-N-acetylglucosamine into UDP-N-acetylgalactosamine, is responsible for the presence of N-acetylgalactosamine in the EPS repeating units of both strains. (hud.ac.uk)
  • It can also be found in the N-terminal region of the mammalian bifunctional protein GNE, which has both the UDP-N-acetylglucosamine 2-epimerase and the N-acetylmannosamine kinase functions. (ebi.ac.uk)
  • Auf www.antikoerper-online.de finden Sie aktuell 44 Methylmalonyl CoA Epimerase (MCEE) Antikörper von 14 unterschiedlichen Herstellern. (antikoerper-online.de)
  • Insgesamt sind aktuell 66 Methylmalonyl CoA Epimerase Produkte verfügbar. (antikoerper-online.de)
  • This family represents a putative epimerase that converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6P) in the N-acetylmannosamine utilization pathway, found mainly in pathogenic bacteria. (ebi.ac.uk)
  • In addition, computer analysis recognized epimerases of various organisms as highly similar to the putative epimerase of V. vulnificus . (asm.org)
  • We recently reported functional and structural studies of D-ribulose 5-phosphate 3-epimerase (RPE) from Streptococcus pyogenes that catalyzes the equilibration of the pentulose 5-phosphates D-ribulose 5-phosphate and D-xylulose 5-phosphate in the pentose phosphate pathway [J. Akana, A. A. Fedorov, E. Fedorov, W. R. P. Novack, P. C. Babbitt, S. C. Almo, and J. A. Gerlt (2006) Biochemistry 45, 2493- (elsevier.com)
  • The expression of the epimerase was confirmed with Western blots using specific anti-epimerase antibodies and by measuring the rate of inversion of ( R )-ibuprofenoyl-CoA. (aspetjournals.org)
  • Cloning, sequence, and transcriptional regulation of the operon encoding a putative N-acetylmannosamine-6-phosphate epimerase (nanE) and sialic acid lyase (nanA) in Clostridium perfringens. (ebi.ac.uk)
  • Novel inhibitors of Trypanosoma brucei and mammalian UDP-Glc 4'-epimerase were identified by screening a small library of natural products and commercially available drug-like molecules. (lancs.ac.uk)
  • The structure from the NAD-dependent oxidoreductase UDP-galactose-4′-epimerase from in complex with cofactor and the substrate analogue UDP-4-deoxy-4-fluoro-α-d-galactose has been identified using diffraction data to 2. (onetownmanyvoices.com)
  • Here we propose to pursue a novel target for anti-TB drugs which was recently and fully validated, namely decaprenyl-phosphoryl ribose epimerase (DprE1), an enzyme that in conjunction with DprE2 catalyses the transformation of decaprenyl-phosphoryl ribose into decaprenyl-phosphoryl arabinose, in the arabinan biosynthetic pathway in Mycobacterium tuberculosis. (europa.eu)
  • To clarify this mechanism, the sequence of the 2-arylpropionyl-CoA epimerase was identified, and the enzyme cloned and expressed. (aspetjournals.org)
  • Uridinediphosphoglucose (UDPG) dehydrogenase, UDPG epimerase and UDP-glucuronate epimerase activities in H. diminuta were highest in subtegumental parenchyma, indicating that this is where mucopolysaccharide precursors are synthesized. (eurekamag.com)
  • A cDNA clone encoding the 2-arylpropionyl-CoA epimerase was isolated from a rat liver cDNA library. (aspetjournals.org)
  • Provided is a method of producing D-psicose using a D-psicose epimerase derived from Agrobacterium tumefaciens. (patentsencyclopedia.com)
  • The present invention relates to a method of producing D-psicose using a D-psicose epimerase (hereinafter, referred to as psicose 3-epimerase) derived from Agrobacterium tumefaciens. (patentsencyclopedia.com)
  • According to the abstract released by the U.S. Patent & Trademark Office: "The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. (highbeam.com)
  • Chang S, Duerr B, Serif G (1988) An epimerase-reductase in L-fucose synthesis. (springer.com)
  • Herein we present the structural and biochemical characterization of UDP-galactose-4-epimerase from Aspergillus nidulans which produces the precursor UDP-galactopyranose required for galactofuranose synthesis. (duhnnae.com)
  • UDP-galactose 4-epimerase from yeast (Kluyveromyces fragilis) is a homodimer of total molecular mass 150 kDa having possibly one mole of NAD/dimer acting as a cofactor. (who.int)
  • Mutational studies on the conserved catalytic motif support a similar mechanism to that established for the Human counterpart is likely operational within the A. nidulans epimerase. (duhnnae.com)
  • AlgE7 is special in that it is both an epimerase and an alginate lyase. (bibsys.no)
  • The complete inhibition of the enzyme by 2-nitro-5-thiocyanatobenzoate, 5,5'-dithio-bis(2-nitrobenzoic acid) and 1,2-benzisothiazolidine-3-one at nanomolar concentrations suggested that these sulphydryl alkylating agents modify functionally significant cysteine residues at or near the active site of the epimerase. (warwick.ac.uk)
  • The deduced enzyme is composed of 1610 amino acids, and showed a complex modular structure, A-R1-R2-M-R3-N-RTX-S. The A- and Rmodules are similar to those present in the A. vinelandii AlgE-epimerases, and the A-module was found to be 53-61% identical to the AlgE-A-modules. (bibsys.no)
  • Examination of the structural model revealed both NAD+ and UDP-glucopyranose were bound within the active site cleft in a near identical fashion to that found in the Human epimerase. (duhnnae.com)