A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.
Restoration of integrity to traumatized tissue.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A cell surface protein-tyrosine kinase receptor that is overexpressed in a variety of ADENOCARCINOMAS. It has extensive homology to and heterodimerizes with the EGF RECEPTOR, the ERBB-3 RECEPTOR, and the ERBB-4 RECEPTOR. Activation of the erbB-2 receptor occurs through heterodimer formation with a ligand-bound erbB receptor family member.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Deacetylases that remove N-acetyl groups from amino side chains of the amino acids of HISTONES. The enzyme family can be divided into at least three structurally-defined subclasses. Class I and class II deacetylases utilize a zinc-dependent mechanism. The sirtuin histone deacetylases belong to class III and are NAD-dependent enzymes.
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Formation of an acetyl derivative. (Stedman, 25th ed)
Compounds that inhibit HISTONE DEACETYLASES. This class of drugs may influence gene expression by increasing the level of acetylated HISTONES in specific CHROMATIN domains.
A histone deacetylase subtype that is found along with HISTONE DEACETYLASE 2; RETINOBLASTOMA-BINDING PROTEIN 4; and RETINOBLASTOMA-BINDING PROTEIN 7 as core components of histone deacetylase complexes.
Semisynthetic conjugates of various toxic molecules, including RADIOACTIVE ISOTOPES and bacterial or plant toxins, with specific immune substances such as IMMUNOGLOBULINS; MONOCLONAL ANTIBODIES; and ANTIGENS. The antitumor or antiviral immune substance carries the toxin to the tumor or infected cell where the toxin exerts its poisonous effect.
A protein phytotoxin from the seeds of Ricinus communis, the castor oil plant. It agglutinates cells, is proteolytic, and causes lethal inflammation and hemorrhage if taken internally.
Antibodies produced by a single clone of cells.
A conserved AMINO ACID SEQUENCE located in the intracellular domains of a family of transmembrane proteins involved in various IMMUNE RESPONSES. The CONSENSUS SEQUENCE of this motif is YXXL(or I)X(6-8)YXXL(or I) (where X denotes any amino acid). When phosphorylated ITAM motifs provide docking sites for PROTEIN TYROSINE KINASES of the Syk family thus forming signaling complexes which lead to activation of immune responses.
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
Hormonally active polypeptides that can induce the transformed phenotype when added to normal, non-transformed cells. They have been found in culture fluids from retrovirally transformed cells and in tumor-derived cells as well as in non-neoplastic sources. Their transforming activities are due to the simultaneous action of two otherwise unrelated factors, TRANSFORMING GROWTH FACTOR ALPHA and TRANSFORMING GROWTH FACTOR BETA.
Gonadal interstitial or stromal cell neoplasm composed of only LEYDIG CELLS. These tumors may produce one or more of the steroid hormones such as ANDROGENS; ESTROGENS; and CORTICOSTEROIDS. Clinical symptoms include testicular swelling, GYNECOMASTIA, sexual precocity in children, or virilization (VIRILISM) in females.
A protein-tyrosine kinase receptor that is closely related in structure to the INSULIN RECEPTOR. Although commonly referred to as the IGF-I receptor, it binds both IGF-I and IGF-II with high affinity. It is comprised of a tetramer of two alpha and two beta subunits which are derived from cleavage of a single precursor protein. The beta subunit contains an intrinsic tyrosine kinase domain.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Signal molecules that are involved in the control of cell growth and differentiation.
A storage reservoir for BILE secretion. Gallbladder allows the delivery of bile acids at a high concentration and in a controlled manner, via the CYSTIC DUCT to the DUODENUM, for degradation of dietary lipid.
Series of ocean waves produced by geologic events or underwater LANDSLIDES. These waves can travel at speeds averaging 450 (and up to 600) miles per hour in the open ocean.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Sudden slips on a fault, and the resulting ground shaking and radiated seismic energy caused by the slips, or by volcanic or magmatic activity, or other sudden stress changes in the earth. Faults are fractures along which the blocks of EARTH crust on either side have moved relative to one another parallel to the fracture.
Tumors or cancer of the gallbladder.

Growth inhibition of breast cancer cells by Grb2 downregulation is correlated with inactivation of mitogen-activated protein kinase in EGFR, but not in ErbB2, cells. (1/7488)

Increased breast cancer growth has been associated with increased expression of epidermal growth factor receptor (EGFR) and ErbB2 receptor tyrosine kinases (RTKs). Upon activation, RTKs may transmit their oncogenic signals by binding to the growth factor receptor bound protein-2 (Grb2), which in turn binds to SOS and activates the Ras/Raf/MEK/mitogen-activated protein (MAP) kinase pathway. Grb2 is important for the transformation of fibroblasts by EGFR and ErbB2; however, whether Grb2 is also important for the proliferation of breast cancer cells expressing these RTKs is unclear. We have used liposomes to deliver nuclease-resistant antisense oligodeoxynucleotides (oligos) specific for the GRB2 mRNA to breast cancer cells. Grb2 protein downregulation could inhibit breast cancer cell growth; the degree of growth inhibition was dependent upon the activation and/or endogenous levels of the RTKs. Grb2 inhibition led to MAP kinase inactivation in EGFR, but not in ErbB2, breast cancer cells, suggesting that different pathways might be used by EGFR and ErbB2 to regulate breast cancer growth.  (+info)

The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579. (2/7488)

The extracellular receptor stimulated kinase ERK2 (p42(MAPK))-phosphorylated human cAMP-specific phosphodiesterase PDE4D3 at Ser579 and profoundly reduced ( approximately 75%) its activity. These effects could be reversed by the action of protein phosphatase PP1. The inhibitory state of PDE4D3, engendered by ERK2 phosphorylation, was mimicked by the Ser579-->Asp mutant form of PDE4D3. In COS1 cells transfected to express PDE4D3, challenge with epidermal growth factor (EGF) caused the phosphorylation and inhibition of PDE4D3. This effect was blocked by the MEK inhibitor PD98059 and was not apparent using the Ser579-->Ala mutant form of PDE4D3. Challenge of HEK293 and F442A cells with EGF led to the PD98059-ablatable inhibition of endogenous PDE4D3 and PDE4D5 activities. EGF challenge of COS1 cells transfected to express PDE4D3 increased cAMP levels through a process ablated by PD98059. The activity of the Ser579-->Asp mutant form of PDE4D3 was increased by PKA phosphorylation. The transient form of the EGF-induced inhibition of PDE4D3 is thus suggested to be due to feedback regulation by PKA causing the ablation of the ERK2-induced inhibition of PDE4D3. We identify a novel means of cross-talk between the cAMP and ERK signalling pathways whereby cell stimuli that lead to ERK2 activation may modulate cAMP signalling.  (+info)

p50(cdc37) acting in concert with Hsp90 is required for Raf-1 function. (3/7488)

Genetic screens in Drosophila have identified p50(cdc37) to be an essential component of the sevenless receptor/mitogen-activated kinase protein (MAPK) signaling pathway, but neither the function nor the target of p50(cdc37) in this pathway has been defined. In this study, we examined the role of p50(cdc37) and its Hsp90 chaperone partner in Raf/Mek/MAPK signaling biochemically. We found that coexpression of wild-type p50(cdc37) with Raf-1 resulted in robust and dose-dependent activation of Raf-1 in Sf9 cells. In addition, p50(cdc37) greatly potentiated v-Src-mediated Raf-1 activation. Moreover, we found that p50(cdc37) is the primary determinant of Hsp90 recruitment to Raf-1. Overexpression of a p50(cdc37) mutant which is unable to recruit Hsp90 into the Raf-1 complex inhibited Raf-1 and MAPK activation by growth factors. Similarly, pretreatment with geldanamycin (GA), an Hsp90-specific inhibitor, prevented both the association of Raf-1 with the p50(cdc37)-Hsp90 heterodimer and Raf-1 kinase activation by serum. Activation of Raf-1 via baculovirus coexpression with oncogenic Src or Ras in Sf9 cells was also strongly inhibited by dominant negative p50(cdc37) or by GA. Thus, formation of a ternary Raf-1-p50(cdc37)-Hsp90 complex is crucial for Raf-1 activity and MAPK pathway signaling. These results provide the first biochemical evidence for the requirement of the p50(cdc37)-Hsp90 complex in protein kinase regulation and for Raf-1 function in particular.  (+info)

The Jun kinase 2 isoform is preferentially required for epidermal growth factor-induced transformation of human A549 lung carcinoma cells. (4/7488)

We have previously found that epidermal growth factor (EGF) mediates growth through the Jun N-terminal kinase/stress-activated kinase (JNK/SAPK) pathway in A549 human lung carcinoma cells. As observed here, EGF treatment also greatly enhances the tumorigenicity of A549 cells, suggesting an important role for JNK in cancer cell growth (F. Bost, R. McKay, N. Dean, and D. Mercola, J. Biol. Chem. 272:33422-33429, 1997). Several isoforms families of JNK, JNK1, JNK2, and JNK3, have been isolated; they arise from alternative splicing of three different genes and have distinct substrate binding properties. Here we have used specific phosphorothioate oligonucleotides targeted against the two major isoforms, JNK1 and JNK2, to discriminate their roles in EGF-induced transformation. Multiple antisense sequences have been screened, and two high-affinity and specific candidates have been identified. Antisense JNK1 eliminated steady-state mRNA and JNK1 protein expression with a 50% effective concentration (EC50) of <0.1 microM but did not alter JNK2 mRNA or protein levels. Conversely, antisense JNK2 specifically eliminated JNK2 steady-state mRNA and protein expression with an EC50 of 0.1 microM. Antisense JNK1 and antisense JNK2 inhibited by 40 and 70%, respectively, EGF-induced total JNK activity, whereas sense and scrambled-sequence control oligonucleotides had no effect. The elimination of mRNA, protein, and JNK activities lasted 48 and 72 h following a single Lipofectin treatment with antisense JNK1 and JNK2, respectively, indicating sufficient duration for examining the impact of specific elimination on the phenotype. Direct proliferation assays demonstrated that antisense JNK2 inhibited EGF-induced doubling of growth as well as the combination of active antisense oligonucleotides did. EGF treatment also induced colony formation in soft agar. This effect was completely inhibited by antisense JNK2 and combined-antisense treatment but not altered by antisense JNK1 alone. These results show that EGF doubles the proliferation (growth in soft agar as well as tumorigenicity in athymic mice) of A549 lung carcinoma cells and that the JNK2 isoform but not JNK1 is utilized for mediating the effects of EGF. This study represents the first demonstration of a cellular phenotype regulated by a JNK isoform family, JNK2.  (+info)

Expression and differential regulation of connective tissue growth factor in pancreatic cancer cells. (5/7488)

CTGF is an immediate early growth responsive gene that has been shown to be a downstream mediator of TGFbeta actions in fibroblasts and vascular endothelial cells. In the present study hCTGF was isolated as immediate early target gene of EGF/TGFalpha in human pancreatic cancer cells by suppression hybridization. CTGF transcripts were found in 13/15 pancreatic cancer cell lines incubated with 10% serum. In 3/7 pancreatic cancer cell lines EGF/TGFalpha induced a significant rise of CTGF transcript levels peaking 1-2 h after the start of treatment. TGFbeta increased CTGF transcript levels in 2/7 pancreatic cancer cell lines after 4 h of treatment and this elevation was sustained after 24 h. Only treatment with TGFbeta was accompanied by a parallel induction of collagen type I transcription. 15/19 human pancreatic cancer tissues were shown to overexpress high levels of CTGF transcripts. CTGF transcript levels in pancreatic cancer tissues and nude mouse xenograft tumors showed a good correlation to the degree of fibrosis. In situ hybridization and the nude mouse experiments revealed that in pancreatic cancer tissues, fibroblasts are the predominant site of CTGF transcription, whereas the tumor cells appear to contribute to a lesser extent. We conclude that CTGF may be of paramount importance for the development of the characteristic desmoplastic reaction in pancreatic cancer tissues.  (+info)

Thyroid hormone promotes the phosphorylation of STAT3 and potentiates the action of epidermal growth factor in cultured cells. (6/7488)

We have examined the effects of l-thyroxine (T4) on the activation of signal transducer and activator of transcription 3 (STAT3) and on the STAT3-dependent induction of c-Fos expression by epidermal growth factor (EGF). T4, at a physiological concentration of 100 nM, caused tyrosine phosphorylation and nuclear translocation (i.e. activation) of STAT3 in HeLa cells in as little as 10-20 min. Activation by T4 of STAT3 was maximal at 30 min (15+/-4-fold enhancement; mean+/-S.E.M.) in 18 experiments. This effect was reproduced by T4-agarose (100 nM) and blocked by CGP 41251, genistein, PD 98059 and geldanamycin, inhibitors of protein kinase C (PKC), protein tyrosine kinase (PTK), mitogen-activated protein kinase (MAPK) kinase and Raf-1 respectively. Tyrosine-phosphorylated MAPK also appeared in nuclear fractions within 10 min of treatment with T4. In the nuclear fraction of T4-treated cells, MAPK immunoprecipitate also contained STAT3. The actions of T4 were similar in HeLa and CV-1 cells, which lack thyroid hormone receptor (TR), and in TR-replete skin fibroblasts (BG-9). T4 also potentiated the EGF-induced nuclear translocation of activated STAT1alpha and STAT3 and enhanced the EGF-stimulated expression of c-Fos. Hormone potentiation of EGF-induced signal transduction and c-Fos expression was inhibited by CGP 41251, geldanamycin and PD 98059. Therefore the non-genomically induced activation by T4 of STAT3, and the potentiation of EGF by T4, require activities of PKC, PTK and an intact MAPK pathway.  (+info)

Activities of glucose metabolic enzymes in human preantral follicles: in vitro modulation by follicle-stimulating hormone, luteinizing hormone, epidermal growth factor, insulin-like growth factor I, and transforming growth factor beta1. (7/7488)

Modulation of glucose metabolic capacity of human preantral follicles in vitro by gonadotropins and intraovarian growth factors was evaluated by monitoring the activities of phosphofructokinase (PFK) and pyruvate kinase (PK), two regulatory enzymes of the glycolytic pathway, and malate dehydrogenase (MDH), a key mitochondrial enzyme of the Krebs cycle. Preantral follicles in classes 1 and 2 from premenopausal women were cultured separately in vitro in the absence or presence of FSH, LH, epidermal growth factor (EGF), insulin-like growth factor (IGF-I), or transforming growth factor beta1 (TGFbeta1) for 24 h. Mitochondrial fraction was separated from the cytosolic fraction, and both fractions were used for enzyme assays. FSH and LH significantly stimulated PFK and PK activities in class 1 and 2 follicles; however, a 170-fold increase in MDH activity was noted for class 2 follicles that were exposed to FSH. Although both EGF and TGFbeta1 stimulated glycolytic and Krebs cycle enzymes for class 1 preantral follicles, TGFbeta1 consistently stimulated the activities of both glycolytic enzymes more than that of EGF. IGF-I induced PK and MDH activities in class 1 follicles but negatively influenced PFK activity for class 1 follicles. In general, only gonadotropins consistently stimulated both glycolytic and Krebs cycle enzyme activities several-fold in class 2 follicles. These results suggest that gonadotropins and ovarian growth factors differentially influence follicular energy-producing capacity from glucose. Moreover, gonadotropins may either directly influence glucose metabolism in class 2 preantral follicles or do so indirectly through factors other than the well-known intraovarian growth factors. Because growth factors modulate granulosa cell mitosis and functionality, their role on energy production may be related to specific cellular activities.  (+info)

Recovery following relief of unilateral ureteral obstruction in the neonatal rat. (8/7488)

BACKGROUND: Obstructive nephropathy is a primary cause of renal insufficiency in infants and children. This study was designed to distinguish the reversible and irreversible cellular consequences of temporary unilateral ureteral obstruction (UUO) on the developing kidney. METHODS: Rats were subjected to UUO or sham operation in the first 48 hours of life, and the obstruction was removed five days later (or was left in place). Kidneys were removed for study 14 or 28 days later. In additional groups, kidneys were removed at the end of five days of obstruction. Immunoreactive distribution of renin was determined in arterioles, and the distribution of epidermal growth factor, transforming growth factor-beta1, clusterin, vimentin, and alpha-smooth muscle actin was determined in tubules and/or interstitium. The number of glomeruli, glomerular maturation, tubular atrophy, and interstitial collagen deposition was determined by morphometry. Renal cellular proliferation and apoptosis were measured by proliferating cell nuclear antigen and the TdT uridine-nick-end-label technique, respectively. The glomerular filtration rate was measured by inulin clearance. RESULTS: Renal microvascular renin maintained a fetal distribution with persistent UUO; this was partially reversed by the relief of obstruction. Although glomerular maturation was also delayed and glomerular volume was reduced by UUO, the relief of obstruction prevented the reduction in glomerular volume. Although relief of obstruction did not reverse a 40% reduction in the number of nephrons, the glomerular filtration rate of the postobstructed kidney was normal. The relief of obstruction did not improve tubular cell proliferation and only partially reduced apoptosis induced by UUO. This was associated with a persistent reduction in the tubular epidermal growth factor. In addition, the relief of obstruction reduced but did not normalize tubular expression of transforming growth factor-beta1, clusterin, and vimentin, all of which are evidence of persistent tubular injury. The relief of obstruction significantly reduced interstitial fibrosis and expression of alpha-smooth muscle actin by interstitial fibroblasts, but not to normal levels. CONCLUSIONS: The relief of obstruction in the neonatal rat attenuates, but does not reverse, renal vascular, glomerular, tubular, and interstitial injury resulting from five days of UUO. Hyperfiltration by remaining nephrons and residual tubulointerstitial injury in the postobstructed kidney are likely to lead to deterioration of renal function later in life.  (+info)

A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. Recombinant Human Epidermal Growth Factor produced in E.Coli is a single, non-glycosylated, polype
BACKGROUND AND AIMS: Neonatal necrotizing enterocolitis (NEC) is a common and serious acquired gastrointestinal tract condition. This clinical study assessed the potential clinical efficacy and microscopic effects of recombinant human epidermal growth factor 1-48 (EGF(1-48)) in neonates with NEC. METHODS: This prospective, double-blind, randomized controlled study included 8 neonates with NEC. The study compared the effects of a 6-day continuous intravenous infusion of EGF(1-48) at 100 ng kg(-1) h(-1) against placebo. Clinical outcomes and morphological evaluation of serial rectal mucosal biopsies were assessed at baseline and 4, 7, and 14 days after starting EGF infusions. RESULTS: There was no difference between the clinical safety outcomes recorded for EGF(1-48) or placebo patients. Quantitative morphologic differences in the rectal mucosa biopsies were noted with EGF(1-48) treatment compared with baseline or placebo and included a statistically significant increase in the number of mitoses per
mouse epidermal growth factor binding protein: four different mouse proteins have been identified as EGF-BP; each is a kallikrein that associates with EGF to form a high molecular weight complex in male mouse submandibular glands (separate SCRs for each of four proteins exist)
Background: Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore the effect of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF.|st...
When the serum content of tissue culture medium is reduced from 10% to 1%, the capacity of T cells to proliferate in response to antigen within that medium is dramatically reduced. Physiological concentrations of platelet-derived growth factor (PDGF) or epidermal growth factor (EGF) are able to partially replace the requirement for serum, in that they are able to increase antigen-driven T-cell proliferation at a serum concentration of 1%. Neither growth factor is mitogenic for T cells in the absence of antigen, and neither is able to act synergistically with T-cell growth factor (TCGF) or IL-2) in the absence of antigen. Antigen-presenting cells (APC) pulsed with antigen in the presence of PDGF or EGF are able to stimulate antigen-specific T-cell proliferation to a greater extent than antigen-presenting cells pulsed in the absence of exogenous PDGF or EGF. Both growth factors increase the expression of MHC Class II antigens on antigen-presenting cells.
Integrins play a critical role in carcinogenesis. Integrin β1 localization is regulated by the guanosine-5′-triphosphate hydrolase Rab25 and integrin β1 levels are elevated in the serum of colon cancer patients; thus, the present study examined the effects of epidermal growth factor (EGF) and Rab25 on integrin β1 localization in colon cancer cells. HCT116 human colon cancer cells were treated with increasing concentrations of EGF, and cell proliferation and protein expression were monitored by MTT and western blot analyses, respectively. Cell fractionation was performed to determine integrin β1 localization in the membrane and cytosol. Integrin β1 extracellular shedding was monitored by enzyme-linked immunosorbent assays (ELISAs) with culture supernatants from stimulated cells. HCT116 cells were transfected with Rab25-specific siRNA to determine the significance of Rab25 in integrin β1 trafficking in the presence of EGF. Total integrin β1 expression increased in response to EGF and subsequently
AIM: To evaluate the effects of epidermal growth factor (EGF) on transforming growth factor-beta1 (TGF- 1)-induced epithelial-mesenchymal transition (EMT) in human corneal epithelial cells (HCECs). METHODS: HCECs were cultured and treated with TGF- 1 for establishing the model of EMT in vitro. Biological effect of EGF on TGF- 1-induced EMT was evaluated. Proteins and mRNAs expression changes of E-cadherin, N-cadherin and Fibronectin (EMT-relative markers) after TGF- 1 or TGF- 1 combined EGF treatment were detected by Western blot and RT-PCR, respectively. Viability and migration of HCECs were measured by CCK-8, transwell cell migration assay and cell scratch wound healing assay. Activation of Smad2, ERK, p38, JNK and Akt signaling pathways were evaluated by Western blot. Inhibitors of relevant signaling pathways were added to the HCECs to explore the key signal mechanism. RESULTS: With treatment of TGF- 1 only, three EMT-relative proteins and mRNA expression showed that EMT up-regulated in a
1EGF: Solution structure of murine epidermal growth factor determined by NMR spectroscopy and refined by energy minimization with restraints.
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EPIDERMAL GROWTH FACTOR (EGF) IS A POTENT MITOGEN FOR A VARIETY OF CELL TYPES BOTH IN VITRO AND IN VIVO. RECENT STUDIES HAVE SUGGESTED THAT EGF MAY PLAY A ROLE IN THE MAINTENANCE OF NORMAL PREGNANCY IN MICE. REMOVAL OF THE SUBMANDIBULAR GLAND OF PREGESTATIONAL MICE ATTENUATES THE NORMAL RISE IN PLASMA EGF DURING GESTATION AND REDUCES THE NUMBER OF MICE COMPLETING TERM PREGNANCY BY 50%, AN EGF REPLACEMENT THERAPY SUBSTANTIALLY IMPROVES THE OUTCOME OF PREGNANCY IN THESE MICE. EGF LEVELS ARE KNOWN TO BE ELEVATED THROUGHOUT HUMAN PREGNANCY. HOWEVER, THE ROLE OF EGF EXPRESSION IN THE SUCCESSFUL COMPLETION OF FULL-TERM FETAL DEVELOPMENT IN *HUMANS HAS NOT BEEN FULLY EXPLORED. THE GOAL OF THIS PROGRAM IS TO DETERMINE WHETHER *DIAGNOSTICMEASUREMENTS OF MATERNAL EGF LEVELS WILL PROVE USEFUL IN THEIDENTIFICATION AND MANAGEMENT OF HIGH RISK PREGNANCIES. IN PHASE I WE WILL UTILIZE A SERIES OF MONOCLONAL ANTIBODIES, PRODUCED PREVIOUSLY, TO DEVELOP A HIGHLY SENSITIVE IMMUNOMETRIC ASSAY FOR HUMAN *EGF. THE ...
Cancer cells have different characteristics due to the genetic differences where these unique features may strongly influence the effectiveness of therapeutic interventions. Here, we show that the spontaneous reactivation of extracellular signal-regulated kinase (ERK), distinct from conventional ERK activation, represents a potent mechanism for cancer cell survival. We studied ERK1/2 activation in vitro in SW480 colorectal cancer cells. Although ERK signaling tends to be transiently activated, we observed the delayed reactivation of ERK1/2 in epidermal growth factor (EGF)-stimulated SW480 cells. This effect was observed even after EGF withdrawal. While phosphorylated ERK1/2 translocated into the nucleus following its primary activation, it remained in the cytoplasm during late-phase activation. The inhibition of primary ERK1/2 activation or protein trafficking, blocked reactivation and concurrently increased caspase 3 activity. Our results suggest that the biphasic activation of ERK1/2 plays a ...
Sigma-Aldrich offers abstracts and full-text articles by [Koji Yamamoto, Kohta Takahashi, Kazuhiro Shiozaki, Kazunori Yamaguchi, Setsuko Moriya, Masahiro Hosono, Hiroshi Shima, Taeko Miyagi].
Epidermal growth factor (EGF) efficiently stimulates expansion of mouse and rat oocyte-cumulus complexes (OCC). Contradictory data have been published by several laboratories about the ability of EGF to stimulate expansion of porcine OCC. We assumed that these contradictions may have resulted from heterogeneous conditions used for isolation, culture, and assessment of OCC. The present experiments were designed to test the hypothesis that porcine OCC acquire the ability to synthesize hyaluronic acid (HA) and undergo expansion following EGF-stimulation gradually during the growth of follicles. For this reason, we isolated OCC from follicles of different sizes and assessed quantity of produced HA and proportions of expanding OCC after stimulation by EGF. In addition, we assessed in those OCC changes in morphology of cumulus cells and assembly of F-actin microfilaments, which are necessary for expansion to occur. Finally, nuclear maturation of EGF-stimulated OCC was assessed and its relationship with
TY - JOUR. T1 - Epidermal growth factor (EGF) extends life span of normal human fibroblasts in culture. AU - Ladda, R. L.. AU - Gianopoulos, T.. AU - McCormick, L.. PY - 1978/1/1. Y1 - 1978/1/1. UR - http://www.scopus.com/inward/record.url?scp=18144443106&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=18144443106&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:18144443106. VL - 12. JO - Pediatric Research. JF - Pediatric Research. SN - 0031-3998. IS - 4 II. ER - ...
We have used biochemical and morphological techniques to demonstrate that hepatocytes in the perfused liver bind, internalize, and degrade substantial amounts of murine epidermal growth factor (EGF) via a receptor-mediated process. Before ligand exposure, about 300,000 high-affinity receptors were detectable per cell, displayed no latency, and co-distributed with conventional plasma membrane markers. Cytochemical localization using EGF coupled to horseradish peroxidase (EGF-HRP) revealed that the receptors were distributed along the entire sinusoidal and lateral surfaces of hepatocytes. When saturating concentrations of EGF were perfused through a liver at 35 degrees C, ligand clearance was biphasic with a rapid primary phase of 20,000 molecules/min per cell that dramatically changed at 15-20 min to a slower secondary phase of 2,500 molecules/min per cell. During the primary phase of uptake, approximately 250,000 molecules of EGF and 80% of the total functional receptors were internalized into ...
Savoji, Houman, Maire, Marion, Lequoy, Pauline, Liberelle, Benoît, de Crescenzo, Gregory, Wertheimer, Michael R., Ajji, Abdellah et Lerouge, Sophie. 2016. « Chondroitin sulfate-oriented epidermal growth factor (EGF) coating for random and aligned electrospun vascular grafts ». Affiche numéro P.2214 présentée lors de la conférence : 10th World Biomaterials Congress (WBC) (Montréal, QC, Canada, May 17-22, 2016). The full text of this document is not available here ...
Programmed cell death ligand 1 (PD-L1) is an important immune-inhibitory protein expressed on cancer cells to mediate cancer escape through interaction with PD-1 expressed on activated T lymphocytes (T cells). Previously, we reported that colon and breast cancer stem cells (CSCs) expressed much higher levels of PD-L1 than their parental cells, suggesting they will be more resistant to immune attack. We investigated the underlining mechanism of PD-L1 increase in colon CSCs, with a special focus on the effect of insulin and epithelial growth factor (EGF), the two fundamental components to sustain the metabolism and stemness in the culture of CSCs. We found that insulin increased the total and surface PD-L1 levels through PI3K/Akt/mTOR pathway as the increase could be inhibited by the dual inhibitor of the pathway, BEZ235. EGF didnt affect the total PD-L1 levels of CSCs but increased the cell surface protein levels by flow cytometry analysis, indicating EGF promotes the transport of PD-L1 to the cell
Recombinant Mouse EGF Protein (Asn977-Arg1029) 50482-MNAY is expressed in Yeast. With high purity, high biological activity, high stability, and other superior features, you can use this Mouse EGF protein for relevant bioassay and related research.
TY - JOUR. T1 - Equine oocyte maturation with epidermal growth factor. AU - Lorenzo, P. L.. AU - Liu, Irwin. AU - Carneiro, G. F.. AU - Conley, Alan J. AU - Enders, A. C.. PY - 2002/7. Y1 - 2002/7. N2 - Epidermal growth factor (EGF) has been shown to have a positive effect during oocyte in vitro maturation in several species. This study was performed to establish the capacity of equine oocytes to undergo nuclear maturation in the presence of EGF and to localise its receptor in the equine ovary by immunohistochemical methods. Oocytes were obtained by aspiration and subsequent scraping from equine follicles (15-25 mm diameter) and cultured in 3 different treatment groups for 36 h: control Group (modified TCM 199 with 0.003% BSA), EGF Group (TCM-199 supplemented with 50 ng/ml EGF) and EMS Group (TCM 199 supplemented with 10% v/v oestrous mare serum). Each group was divided further into 3 treatments with tyrphostin A-47, a specific tyrosine kinase inhibitor, at 0, 10-4 and 10-6 mmol/l. Maturation ...
The complete amino acid sequence of rat transforming growth factor type 1 has been determined. This growth factor, obtained from retrovirus-transformed fibroblasts, is structurally and functionally related to mouse epidermal growth factor and human urogastrone. Production of this polypeptide by various neoplastic cells might contribute to the continued expression of the transformed phenotype. ...
TY - JOUR. T1 - Transforming growth factor β and epidermal growth factor alter calcium influx and phosphatidylinositol turnover in Rat-1 fibroblasts. AU - Muldoon, L. L.. AU - Rodland, K. D.. AU - Magun, B. E.. PY - 1988. Y1 - 1988. N2 - Transforming growth factor type β (TGFβ) alters the cellular response to epidermal growth factor (EGF) for a variety of processes ranging from early transport activities and gene transcription to mitogenesis. In order to test the hypothesis that altered signal transduction mechanisms may mediate both the transforming effects of TGFβ and the modulation of EGF-stimulated processes by TGFβ, we have examined second messenger levels in response to growth factor treatment. The addition of EGF or prolonged treatment with TGFβ increased the rate of 45Ca influx in serum-deprived, confluent Rat-1 cells, while the addition of EGF to TGFβ-pretreated cells produced an additive increase in Ca2+ influx. The stimulation of Ca2+ influx by TGFβ was only observed at ...
TY - JOUR. T1 - Long-term nonpassaged EGF-responsive neural precursor cells are stem cells. AU - Zhou, Feng C.. AU - Chiang, Yung H.. PY - 1998. Y1 - 1998. N2 - We have screened lines of nonpassaged epidermal growth factor-responsive neurospheres from embryonic striatum and brainstem. They have been maintained in defined medium with epidermal growth factor over a period of 2 years and remained in an undifferentiated state to this date. Since isolation from the brain 2 years ago, these nonpassaged epidermal growth factor responsive neurospheres have shown active proliferation and self-renewal capacity. When subplated on a poly-D-lysine coated surface, they resumed differentiation within 24 hours. The differentiation process of the nonpassaged epidermal growth factor responsive neurosphere appeared to recapitulate the neural development in the brain. Many cells migrated, extending radial processes while expressing nestin and S100 in the early 7-day subplating culture. They continued to ...
Human epidermal growth factor, also known as EGF, is a 6kD protein with 53 amino acid residues and three intramolecular disulfide bonds. By binding with high affinity to epidermal growth fact receptor (EGFR) on the cell surface, EGF results in a vari
The Human Epidermal Growth Factor Receptor (EGFR/HER1) can be activated by several ligands including Transforming Growth Factor alpha (TGF-α) and Epidermal Growth Factor (EGF). Following ligand binding, EGFR heterodimerizes with other HER family members, such as HER2 (human epidermal growth factor receptor-2). Previously, we showed that the EGFR is upregulated in trastuzumab resistant HER2 positive (HER2+) breast cancer cells. This study is aimed to determine the downstream effects on transcription following EGFR upregulation in HER2+ breast cancer cells. RNA-sequence and ChIP-sequence for H3K18ac and H3K27ac (Histone H3 lysine K18 and K27 acetylation) were conducted following an Epidermal Growth Factor (EGF) treatment time course in HER2+ breast cancer cells, SKBR3. The levels of several proteins of interest were confirmed by western blot analysis. The cellular localization of proteins of interest was examined using biochemically fractionated lysates followed by western blot analysis. Over the course
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Antibodies for proteins involved in positive regulation of epidermal growth factor-activated receptor activity pathways, according to their Panther/Gene Ontology Classification
Antibodies for proteins involved in regulation of epidermal growth factor-activated receptor activity pathways, according to their Panther/Gene Ontology Classification
Purified epidermal growth factor (EGF), isolated from mice submaxillary glands, was used to study regeneration of rabbit corneal epithelium. The progressive decrease in area of standardized 7 mm. central comeal epithelial wounds was determined by serial standardized photography. The projected fluorescein-stained area was measured by planimetry. It has been found that EGF in the concentrations studied (0.05 mg. per milliliter; 0.5 mg. per milliliter; and 2.0 mg. per milliliter) when given topically four times daily, increased the corneal epithelial healing rate compared to saline controls. A 40-fold variation of EGF concentrations failed to effect a statistically significant change in corneal epithelial healing rate. No sign of toxicity was detected clinically and histologically with topical application of EGF on rabbit corneas with intact epithelium and on corneas denuded of epithelium ...
Purpose : Activation of the Epidermal Growth Factor Receptor (EGFR) is critical in corneal epithelial regeneration and homeostasis. The clinical use of EGFR ligands (e.g. epidermal growth factor - EGF) are not reliable tools to restore and maintain the epithelial layer, likely due to high endogenous EGF levels and intrinsic mechanisms of receptor down-regulation. One mediator of EGFR down-regulation is the E3 ubiquitin ligase, c-Cbl. Ubiquitylation of the EGFR targets it for lysosomal degradation; using RNAi to attenuate c-Cbl expression enhanced the kinetics of corneal epithelial wound healing. We postulate that supplementing the relatively high, endogenous levels of EGF (~2-3 ng/ml) in human tears with a c-Cbl antagonist will prevent EGFR down-regulation and enhance receptor activity and promote homeostasis of the epithelial layer. In this study, we identify and test potential c-Cbl antagonist for binding and inhibitory activity ...
Cells and reagents. Human proximal tubular epithelial cells. HK-2 cells, which have been well characterized (60), were obtained from the American Type Culture Collection (ATCC). Monolayers of HK-2 cells were grown on 6-well plates (Corning-Costar, Corning Life Sciences) that were precoated with 5 μg/cm2 type 1 collagen (Rat Tail Collagen Type 1, Invitrogen, Thermo Fisher Scientific) and incubated at 37°C with 5% CO2 and 95% air in keratinocyte serum-free medium (K-SFM) (Gibco, Invitrogen, Thermo Fisher Scientific) supplemented with recombinant human epidermal growth factor (5 ng/ml) and bovine pituitary extract (50 μg/ml). Medium was exchanged at 48-hour intervals, and the cells were not use beyond 25 to 30 passages.. Human immunoglobulin FLCs. Six unique human monoclonal FLCs (three and three) were purified using standard methods from the urine of patients with multiple myeloma, light chain proteinuria, and clinical evidence of significant renal damage that was presumed to be cast ...
Cells and reagents. Human proximal tubular epithelial cells. HK-2 cells, which have been well characterized (60), were obtained from the American Type Culture Collection (ATCC). Monolayers of HK-2 cells were grown on 6-well plates (Corning-Costar, Corning Life Sciences) that were precoated with 5 μg/cm2 type 1 collagen (Rat Tail Collagen Type 1, Invitrogen, Thermo Fisher Scientific) and incubated at 37°C with 5% CO2 and 95% air in keratinocyte serum-free medium (K-SFM) (Gibco, Invitrogen, Thermo Fisher Scientific) supplemented with recombinant human epidermal growth factor (5 ng/ml) and bovine pituitary extract (50 μg/ml). Medium was exchanged at 48-hour intervals, and the cells were not use beyond 25 to 30 passages.. Human immunoglobulin FLCs. Six unique human monoclonal FLCs (three and three) were purified using standard methods from the urine of patients with multiple myeloma, light chain proteinuria, and clinical evidence of significant renal damage that was presumed to be cast ...
TY - JOUR. T1 - Modulation of hnRNP A1 protein gene expression by epidermal growth factor in rat-1 cells. AU - Planck, Stephen. AU - Listerud, Mark D.. AU - Buckley, Sharon D.. PY - 1988/12/23. Y1 - 1988/12/23. N2 - The levels of mRNA encoding hnRNP core protein A1 have been compared in quiescent and proliferating Rat-1 embryonic fibroblasts. Northern blot hybridization analyses using probes made from an A1 cDNA clone, λHDP-182, isolated by Cobianchi et al. (J. Biol. Chem. 261:3536-2543 (1986)) indicated that three sizes of poly A+ RNAs, 1.6, 2.0, & 4.0 kb, have extensive sequence homology. The levels of all three A1 RNA species are responsive to the proliferation state of the cells. Stimulation of quiescent Rat-1 cells with serum or epidermal growth factor resulted in a 2- to 5-fold increase in the levels of each of these three RNAs that was evident after 2 hours and reached a peak after about 8 hours. Addition of the protein synthesis inhibitor, cycloheximide, along with epidermal growth ...
Functional polymorphisms in the epidermal growth factor family genes and their relationship to the risk and severity of breast cancer ...
For women of childbearing potential, failure to agree to use a highly effective form of contraception (patient and/or partner, e.g., surgical sterilization) or two effective forms of contraception (a reliable barrier method in conjunction with spermicidal jelly, birth control pills, or contraceptive hormone implants) and to continue its use for the duration of study treatment ...
In this study, we present for the first time a proteome profile of arterial aging in FXBN rats, a well-characterized rodent model with similarities to the human anatomic and physiological phenotype. Using a combination of 2DE and iTRAQ, we identified and characterized 923 proteins within the aortic wall, of which 50 significantly change abundance with aging, some of which also have posttranslational modifications such as phosphorylation and glycosylation. The proteins with significant abundance changes (Table⇑) observed in this study play significant roles in a number of cellular pathways, including cellular movement, cell proliferation and apoptosis, energy metabolism, etc, and may contribute significantly to vascular remodeling with aging. Some of these proteins have been related to aging in other organs, ie, prostate, skeletal muscle, and skin, etc (for an expanded discussion regarding the proteins, see the online data supplement). Only a few proteins have been specifically linked to ...
Abstract of annual report,Effect of Sialoadenectomy on the Level of Circulating Mouse Epidermal Growth Factor (mEGF) and on the Reproductive Function in Male Mice. (1990 ...
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SWISS-MODEL Template Library (SMTL) entry for 1epi.1. THREE-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE STRUCTURES OF MOUSE EPIDERMAL GROWTH FACTOR IN ACIDIC AND PHYSIOLOGICAL PH SOLUTIONS
Crude, delipidated milk and the acid:ethanol extracts of primary human breast tumors contain several activities that biologically resemble transforming growth factors (TGFs) in that they promote the anchorage-independent growth of normal rat kidney and Mm5mt/c1 mouse mammary tumor cells in soft agar. Three major TGF species with isoelectric points (pl) of about 4.0, 6.0-6.5, and 7.0 have been detected in both tumors and milk. The pl 4.0 species from milk has been purified about 10,000-fold by isoelectric focusing and high-performance liquid chromatography. This species, designated milk-derived growth factor II (MDGFII), coelutes from gel filtration columns with an authentic human epidermal growth factor standard when using a low ionic strength eluting buffer. However, on the same column, MDGFII is completely resolved from human epidermal growth factor with high ionic strength eluting buffers. Nevertheless, MDGFII purified by the latter technique still competes with 125I-epidermal growth factor ...
Epidermal growth factor Epidermal growth factor (beta-urogastrone) PDB rendering based on 1ivo. Available structures: 1ivo, 1jl9, 1nql, 1p9j Identifiers
Cell lines. HMEC, 184A1, 184B5, and AB5 normal epithelial cell lines were obtained from Dr. Stampfer (Lawrence Berkeley Laboratories) and cultured in mammary epithelial growth media, including BPE, hydrocortisone, human epidermal growth factor (EGF), insulin, and antibiotics (Cambrex). MCF10A and AB5 cell lines were grown in DMEM/F12 with 5% horse serum, 20 ng/mL EGF, 0.5 μg/mL hydrocortisone, 100 ng/mL cholera toxin, 10 μg/mL insulin, and 1% antibiotics (Sigma). All other breast cancer cell lines were from American Type Culture Collection and grown in DMEM with 10% fetal bovine serum and 1% antibiotics. The 3475 metastatic subline was from Dr. Massague (Memorial Sloan Ketterring Cancer Center). The cadherin-11 (cad-11)/R-cad null MDA-MB-231 cell line (231v) was derived by limiting dilution and was able to grow tumors in severe combined immunodeficient mice fat pads.. Antibodies. A monoclonal R-cad antibody ( 25) was kindly provided by ICOS Corporation. This antibody was raised against a ...
Active Recombinant human EGF protein (Animal Free) is an Escherichia coli Full length protein 971 to 1023 aa range animal free, |=98% purity, | 0.010 Eu/µg endotoxin level and validated in WB, CellAc…
There are no specific protocols for Recombinant human EGF protein (Animal Free) (ab9697). Please download our general protocols booklet
TY - JOUR. T1 - Inhibition of DNA synthesis in rat hepatocytes by platelet-derived type β transforming growth factor. AU - Carr, B. I.. AU - Hayashi, I.. AU - Branum, E. L.. AU - Moses, H. L.. PY - 1986. Y1 - 1986. N2 - Platelet-derived type β transforming growth factor (TGFβ) is a potent inhibitor of DNA synthesis in primary monolayer cultures of adult rat hepatocytes. TGFβ induced a 50% inhibition of epidermal growth factor (EGF)-mediated DNA synthesis at approximately 5 x 1012 M. This inhibition did not appear to be due to a delay in the peak of DNA synthesis or a toxic action, nor could it be overcome by increasing concentrations of the mitogens EGF, insulin, or glucagon. Inhibition was observed either when TGFβ and EGF were continuously present together in the culture medium or when TGFβ was added to the hepatocyte cultures after removal of the EGF stimulant. This observation together with a lack of an inhibitory effect of TGFβ on the binding of 125I-labeled EGF to hepatocytes in ...
Previous work suggested that desensitization of p21ras in response to growth factors such as epidermal growth factor (EGF) results from receptor down-regulation. Here we show that p21ras is desensitized by insulin in 3T3-L1 adipocytes in the continued presence of activated insulin receptors, while loss of epidermal growth factor and platelet-derived growth factor (PDGF) receptors in response to their ligands correlates with p21ras desensitization. Furthermore, elevated amounts of Grb2/Shc complexes persisted throughout p21ras desensitization by insulin. However, immunoblotting of anti-Son-of-sevenless (Sos) 1 and 2 immunoprecipitates with anti-Grb2 antisera revealed that p21ras desensitization in response to insulin and PDGF, but not EGF, is associated with a marked decrease in cellular complexes containing Sos and Grb2 proteins. Nonetheless, the desensitization of p21ras in response to these stimuli was homologous, in that each peptide could reactivate [32P]GTP loading of p21ras after desensitization
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Epidermal growth factor (EGF) is a small mitogenic polypeptide that stimulates the proliferation and differentiation of a wide variety of cells of ectodermal and mesodermal origin, including fibroblasts, epithelial cells, and endothelial cells. MACS® GMP Recombinant Human EGF is designed for ex vivo cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives. - Nederland
In gene expression experiments, hormone receptor (HR)-positive/human epidermal growth factor-2 (HER2)-positive tumors generally cluster within the luminal B subset; whereas HR-negative/HER2-positive tumors reside in the HER2-enriched subset. We investigated whether the clinical behavior of HER2-positive tumors differs by HR status. We evaluated 3,394 patients who presented to National Comprehensive Cancer Network (NCCN) centers with stage I to III HER2-positive breast cancer between 2000 and 2007. Tumors were grouped as HR-positive/HER2-positive (HR+/HER2+) or HR-negative/HER2-positive (HR-/HER2+). Chi-square, logistic regression and Cox hazard proportional regression were used to compare groups. Median follow-up was four years. Patients with HR-/HER2+ tumors (n = 1,379, 41% of total) were more likely than those with HR+/HER-2+ disease (n = 2,015, 59% of total) to present with high histologic grade and higher stages (P |0.001). Recurrences were recorded for 458 patients. HR-/HER2+ patients were less
TY - JOUR. T1 - Inhibition of epidermal growth factor-like growth factor secretion in tracheobronchial epithelial cells by vitamin A. AU - Miller, Lisa. AU - Cheng, Ling Zhong. AU - Wu, Reen. PY - 1993/6/1. Y1 - 1993/6/1. N2 - Vitamin A deficiency of respiratory tract epithelium results in the phenomenon of squamous cell metaplasia. The mechanisms by which vitamin A regulates airway epithelial cell growth and differentiation are not completely understood. In this study, we focused on the effects of vitamin A (retinol) on growth of human and non-human primate tracheobronchial epithelial (TBE) cells in culture. Retinol and its derivatives have little growth-stimulatory effect on TBE cells that are maintained in primary culture in a serum-free medium supplemented with 6 hormonal supple-ments: insulin, transferrin, epidermal growth factor (EGF), hydrocortisone, cholera toxin, and bovine hypothalamus extract. However, it was observed that retinol exhibited dose-dependent inhibition of TBE cell growth ...
Evidence suggests flow-induced arterial remodeling involves factors released from cells that are intrinsic to the vessel wall and recruited from the bloodstream. Understanding the molecular details has been hampered by the need to study the process in vivo. The present findings suggest that HB-EGF, which has primarily been studied in epithelial and tumor cells, plays a pivotal role in low FINR of the mouse carotid artery. Sustained low flow activated or increased the following elements within the HB-EGF signaling pathway: ROS, the ROS-sensitive HB-EGF sheddase TACE, expression of pro-HB-EGF, HB-EGF immunoreactivity, the HB-EGF receptor EGFR, ERK1/2, and the transcription factor NF-κB. These changes were associated with proliferation, increased leukocyte density, wall hypertrophy, and lumen narrowing. Heterozygous and homozygous deletion of HB-EGF alleles caused dose-dependent-like inhibition of FINR (although inhibition of lumen narrowing was in some situations spared (see below), where ...
Epidermal growth factor receptors are present in some breast cancers in man, and there is an inverse relation to oestrogen receptor state. We assessed the presence of epidermal growth factor receptors as a single prognostic indicator in a series of breast tumours by comparing this with the Bloom and Richardson scores for these tumours. One hundred and eight ductal tumours were examined for epidermal growth factor receptors by radioligand binding. There was a significant (p less than 0.01) correlation between the presence of the growth factor receptor and poor prognosis as assessed by the Bloom and Richardson score, suggesting that epidermal growth factor receptor state could be a useful prognostic marker. Epidermal growth factor receptor state was not significantly correlated with the lymph node state but showed a tendency to be associated with large tumours.. ...
TY - JOUR. T1 - Developmental regulation of epidermal growth factor receptor kinase in rat intestine. AU - Thompson, John F.. AU - Van Den Berg, Merlijn. AU - Stokkers, Pieter C.F.. PY - 1994/11. Y1 - 1994/11. N2 - Background/Aims: Intraluminal epidermal growth factor (EGF) may regulate intestinal growth and function. The ontogeny, localization, and phosphorylation of the EGF receptor in rat small intestine were studied. Methods: EGF-receptor phosphorylation was assayed by antiphosphotyrosine Western blot after EGF administration in vivo and EGF incubation to everted sacs in vitro. EGF-receptor abundance and localization were assayed by Western blot and immunofluorescence using anti-EGF-receptor antibodies. Results: In vivo, orogastric EGF enhanced EGF-receptor phosphorylation in newborn rat jejunum and liver. However, intraluminal EGF had no effect on EGF-receptor phosphorylation in adult intestine or liver. In vitro, mucosal EGF stimulated a fourfold increase in EGF-receptor phosphorylation in ...
TY - JOUR. T1 - Domain map of the LDL receptor. T2 - Sequence homology with the epidermal growth factor precursor. AU - Russell, David W.. AU - Schneider, Wolfgang J.. AU - Yamamoto, Tokuo. AU - Luskey, Kenneth L.. AU - Brown, Michael S.. AU - Goldstein, Joseph L.. PY - 1984/6. Y1 - 1984/6. N2 - The nucleotide sequence of a partial cDNA for the bovine low-density lipoprotein (LDL) receptor revealed an open reading frame of 264 amino acids that encodes the COOH-terminal 25% of the receptor protein. The sequence predicts a cytoplasmic domain of 50 amino acids at the COOH terminus, followed in order by a membrane-spanning region of 27 hydrophobic amino acids and an externally disposed stretch of 42 amino acids, that is rich in serine and threonine residues and appears to be the site of O-linked glycosylation. This orientation was confirmed by proteolysis experiments in which the relevant fragments were localized by blotting with antipeptide antibodies and a galactose-specific lectin. The ...
The MET inhibitor INC-280 restored sensitivity to erlotinib and promoted apoptosis in nonCsmall-cell lung cancer choices rendered resistant to erlotinib by hepatocyte growth factor. to revive awareness to erlotinib and promote apoptosis in NSCLC versions rendered erlotinib resistant by HGF. These data give a preclinical rationale for a continuing phase 1 scientific trial of erlotinib plus INC-280 in mutation, among the first identified systems of EGFR TKI level of resistance involves activation from the MET receptor, resulting in restored downstream signaling in both phosphatidylinositol 3-kinase (PI3K)/proteins Rabbit polyclonal to EGFR.EGFR is a receptor tyrosine kinase.Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. kinase B (AKT) and mitogen-activated proteins kinase/extracellular signal-regulated kinase kinase (MEK)/extracellular ...
TY - JOUR. T1 - Role of proneuregulin 1 cleavage and human epidermal growth factor receptor activation in hypertonic aquaporin induction. AU - Herrlich, Andreas. AU - Leitch, Virginia. AU - King, Landon S.. PY - 2004/11/2. Y1 - 2004/11/2. N2 - Mammalian cells are confronted with changes in extracellular osmolality at various sites, including the aqueous layer above the lung epithelium. Hypertonic shock induces the activation of mitogen-activated protein kinases and the expression of a defined set of genes, including aquaporins. We investigated upstream components of the response to hypertonicity in lung epithelial cells and found that before extracellular signal-regulated kinase activation and aquaporin synthesis, the membrane-bound prohormone neuregulin 1-β is cleaved and binds to human epidermal growth factor receptor 3 (HER3). The signaling is prevented by matrix metalloproteinase inhibition, inhibition of neuregulin 1-β binding to HER3, and inhibition of HER tyrosine kinase activity. ...
Osteoblast differentiation is a pivotal event in bone formation. Runt-related transcription factor-2 (Runx2) is an essential factor required for osteoblast differentiation and bone formation. However, the underlying mechanism of Runx2-regulated osteogenic differentiation is still unclear. Here, we explored the corresponding mechanism using the C2C12/Runx2Dox subline, which expresses Runx2 in response to doxycycline (Dox). We found that Runx2-induced osteogenic differentiation of C2C12 cells results in a sustained decrease in the expression of heparin-binding EGF-like growth factor (HB-EGF), a member of the epidermal growth factor (EGF) family. Forced expression of HB-EGF or treatment with HB-EGF is capable of reducing the expression of alkaline phosphatase (ALP), a defined marker of early osteoblast differentiation. HB-EGF-mediated inhibition of ALP depends upon activation of the EGFR and the downstream extracellular signal-regulated kinase, c-Jun N-terminal kinase mitogen-activated protein kinase
TY - JOUR. T1 - Epidermal Growth Factor-Activated Extracellular Signal-Regulated Kinase Suppresses Growth Hormone Expression and Stimulates Proliferation in MtT/E Cells. AU - Nogami, H.. AU - Soya, H.. AU - Hiraoka, Y.. AU - Aiso, Sadakazu. AU - Hisano, S.. PY - 2012/2/1. Y1 - 2012/2/1. N2 - The mechanism for the inhibition of growth hormone (GH) expression by the epidermal growth factor (EGF) was examined in two clonal cell lines, MtT/E and MtT/S. The former has a negligible basal level of GH, whereas the latter has a high basal GH. The treatment of MtT/E cells with retinoic acid resulted in a significant increase in GH mRNA and subsequently GH. This stimulatory response to retinoic acid was strongly suppressed by EGF. This suppression was associated with an increase in the phosphorylation of extracellular signal-regulated kinase 1 and 2 (Erk1/2). The MEK [mitogen-activated protein kinase (MAPK) kinases that activate ERK1 and ERK2] inhibitor, PD98059, clearly inhibited the phosphorylation of ...
TY - JOUR. T1 - Regulation of heparin-binding EGF-like growth factor by miR-212 and acquired cetuximab-resistance in head and neck squamous cell carcinoma. AU - Hatakeyama, Hiromitsu. AU - Cheng, Haixia. AU - Wirth, Pamela. AU - Counsell, Ashley. AU - Marcrom, Samuel R.. AU - Wood, Carey Burton. AU - Pohlmann, Paula R.. AU - Gilbert, Jill. AU - Murphy, Barbara. AU - Yarbrough, Wendell G.. AU - Wheeler, Deric L.. AU - Harari, Paul M.. AU - Guo, Yan. AU - Shyr, Yu. AU - Slebos, Robbert J.. AU - Chung, Christine H.. PY - 2010. Y1 - 2010. N2 - Background: We hypothesized that chronic inhibition of epidermal growth factor receptor (EGFR) by cetuximab, a monoclonal anti-EGFR antibody, induces up-regulation of its ligands resulting in resistance and that microRNAs (miRs) play an important role in the ligand regulation in head and neck squamous cell carcinoma (HNSCC). Methodology/Principal Findings: Genome-wide changes in gene and miR expression were determined in cetuximabsensitive cell line, SCC1, and ...
BioAssay record AID 66433 submitted by ChEMBL: Inhibition of the heparin binding epidermal growth factor (HB-EGF) release from fibrosarcoma HT1080 transfectants expressing alkaline phosphate (AP) tagged HB-EGF stimulated by 12-O-tetradecanoylphorbol 13-acetate(TPA)..
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Title: Targeting Epidermal Growth Factor Receptor in Solid Tumors: Critical Evaluation of the Biological Importance of Therapeutic Monoclonal Antibodies. VOLUME: 16 ISSUE: 29. Author(s):Ch. Gialeli, D. Kletsas, D. Mavroudis, H. P. Kalofonos, G. N. Tzanakakis and N. K. Karamanos. Affiliation:Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26110 Patras, Greece.. Keywords:Epidermal growth factor receptor, solid tumors, colorectal cancer, anti-EGFR therapy, monoclonal antibodies, cetuximab, panitumumab. Abstract: Numerous cellular pathways have a significant impact in the growth and metastatic potential of tumors. Essential element of such pathways is the epidermal growth factor receptor (EGFR), a member of the HER family of receptor tyrosine kinases. One of the most important issues in cancer, which attracted the attention of clinical oncologists, is the potential use of targeted therapies. EGFR signaling pathway is implicated in the control of cell survival, ...
The sequence of the human epidermal growth factor (EGF) receptor shows great homology with the avian erythroblastosis virus v-erb B oncogene, raising the possibility that the receptor gene is identical to the c-erb B protooncogene. Human A431 epidermoid carcinoma cells, which have an unusually high number of EGF receptors, were examined to determine whether elevated EGF receptor levels correlate with gene amplification. Southern blots of genomic DNAs from A431 and other human cell lines were probed with either a v-erb B gene fragment or a human EGF receptor complementary DNA clone (pE7), previously isolated from an A431 complementary DNA library. When either probe was used to analyze Eco RI- or Hind III-generated DNA fragments, EGF receptor DNA sequences were amplified about 30-fold in A431. Differences in the banding pattern of A431 DNA fragments relative to normal fibroblast DNA indicate the occurrence of a rearrangement in the region of the receptor gene. Furthermore, A431 cells contain a ...
We present here evidence that the counterstimulatory chemokine IP-10 affects dermal fibroblast cell responses to growth factors in addition to its known actions on hematopoietic and endothelial cells. We demonstrated that IP-10 inhibits EGFR-mediated motility specifically, likely via a cAMP/PKA-dependent inhibition of EGFR-mediated calpain activation. To our knowledge, this is the first report of the inhibitory effects of IP-10 on growth factor-induced fibroblast motility. These actions implicate a role for IP-10 in limiting fibroblast infiltration during wound healing in response to locally expressed growth factors.. We determined that IP-10 did not disrupt EGFR signaling at the ligand or receptor level. This was expected as (a) both EGF- and HB-EGF-induced motility was similarly inhibited; and (b) EGFR-mediated proliferation was unaffected by IP-10 pretreatment. As cellular response signaling diverges at the immediate postreceptor level with at least one pathway, via PLC-γ, being required for ...
Aida, S., et al., Distribution of epidermal growth factor and epidermal growth factor receptor in human lung: immunohistochemical and immunoelectron-microscopic studies. Respiration, 1994. 61(3):161-166.. Allahverdian, S., et al., Sialyl Lewis X modification of the epidermal growth factor receptor regulates receptor function during airway epithelial wound repair. Clin Exp Allergy, 2010. 40(4):607-618.. Blanchet, S., et al., Fine particulate matter induces amphiregulin secretion by bronchial epithelial cells. Am J Resp Cell Mol Biol, 2004. 30(4):421-427.. Burgel, P.-R. and J.A. Nadel, Epidermal growth factor receptor-mediated innate immune responses and their roles in airway diseases. Eur Resp J, 2008. 32(4):1068-1081.. Ciardiello, F., and Tortora, G. (2008). EGFR antagonists in cancer treatment. N Engl J Med, 2008. 358(11):1160-1174.. Casalino-Matsuda, S.M., et al., Role of hyaluronan and reactive oxygen species in tissue kallikrein-mediated epidermal growth factor receptor activation in human ...
WHI-P154 is first described as a JAK3 inhibitor that displays no activity at JAK1 or JAK2. WHI-P154 inhibits STAT1 activation, iNOS expression and NO production in macrophages in vitro. But it is proved that WHI-P154 also inhibits other common kinases including EGFR, Src, Abl, VEGFR, MAPK and PI3-K and induces apoptosis in human glioblastoma cell lines. [1] WHI-P154 inhibits glioblastoma cell adhesion and migration in the context of ECM.[2] WHI-P154 exhibits significant cytotoxicity against U373 and U87 human glioblastoma cell lines, causing apoptotic cell death at micromolar concentrations. The in vitro antiglioblastoma activity of WHI-P154 is amplified > 200-fold and rendered selective by conjugation to recombinant human epidermal growth factor (EGF). In vitro treatment with EGF-P154 results killing of glioblastoma cells at nanomolar concentrations with an IC50 of 813 nM, whereas no cytotoxicity against EGF-R-negative leukemia cellsis observed, even at concentrations as high as 100 ...
TY - JOUR. T1 - EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts. T2 - Implications for osteolytic bone metastases. AU - Zhu, Ji. AU - Jia, Xun. AU - Xiao, Guozhi. AU - Kang, Yibin. AU - Partridge, Nicola C.. AU - Qin, Ling. PY - 2007/9/14. Y1 - 2007/9/14. N2 - Epidermal growth factor (EGF)-like ligands and their receptors constitute one of the most important signaling networks functioning in normal tissue development and cancer biology. Recent in vivo mouse models suggest this signaling network plays an essential role in bone metabolism. Using a coculture system containing bone marrow macrophage and osteoblastic cells, here we report that EGF-like ligands stimulate osteoclastogenesis by acting on osteoblastic cells. This stimulation is not a direct effect because osteoclasts do not express functional EGF receptors (EGFRs). Further studies reveal that EGF-like ligands strongly regulate the expression of two secreted osteoclast ...
The epidermal growth factor receptor (EGFR) and its ligand transforming growth factor (TGF) alpha are hypothesized to form an autocrine growth loop in non-small cell lung cancer (NSCLC) and to play an important role in tumor formation and progression. We studied the association between overexpression of EGFR, TGF-alpha, or both, and overall survival of patients with resectable NSCLC. Overexpression, defined as ,20% of tumor cells staining on immunohistochemistry, was examined in 96 tumor samples from consecutive patients having resection of previously untreated, well-staged NSCLC who were then followed prospectively (median follow-up, 20.7 months). The expression of three other ligands for EGFR (epidermal growth factor, cripto, and amphiregulin) was examined by Northern analysis to determine whether they might also contribute to a potential growth stimulatory loop. Overall, survival was calculated by the method of Kaplan and Meier, and prognostic factors were compared using the log-rank test. ...
THE ANATOMICAL RECORD 222:191-200 (1988) Radioautographic Demonstration of Receptors for Epidermal Growth Factor in Various Cells of the Oral Cavity MOON-IL CHO, YU LIN LEE, AND PHILIAS R. GARANT Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York at Stony Brook, New York 11794-8700 ABSTRACT Mouse iodinated epidermal growth factor (EGF)was localizedby light and electron microscopic radioautography in basal cells of oral epithelium, papillary cells of the enamel organ, periodontal ligament fibroblasts, preodontoblast precursor cells, and preosteoblasts of the alveolar bone of 13-day-old Sprague-Dawleyrats. The specificity of binding in these cells was suggested by an observed reduction of about 90% in the labeling when excess unlabeled EGF was injected along with the 125I-EGF. In contrast, fully differentiated cells, such as ameloblasts. odontoblasts, and osteoblasts, were only poorly labeled. Quantitative analysis of the light microscopic ...
Additional entry factors have been described more recently such as tyrosine kinase epidermal growth factor-receptor (EGF-R) and Ephrin A2 receptor [15], the Niemann-Pick C1-like 1 receptor [16], the transferrin receptor [17] and the tetraspanin CD63 [18]. an extensive functional study to characterize the ability of these two natural variants to prevent HCV access. We used lentiviral vectors to Centrinone express Wildtype or mutated CLDN6 and OCLN in different cell lines and main human being hepatocytes. HCV illness was then investigated using cell tradition produced HCV particles (HCVcc) as well as HCV pseudoparticles (HCVpp) expressing envelope proteins from different genotypes. Our results show that variants of CLDN6 and OCLN indicated separately or in combination did not impact HCV illness nor cell-to-cell transmission. Hence, our study highlights the difficulty of HCV resistance mechanisms supporting the fact that this process probably not primarily involves HCV access factors and that ...
Integrin signalling co-ordinates with signalling originating from growth factor receptors in the co-operative control of cell proliferation, survival and migration. Increasing evidence suggests that integrins form physical complexes at the cell membrane with growth factor receptors, giving rise to signalling platforms at the adhesive sites. It is probable that at these sites integrins regulate adhesion and at the same time physically constrain and direct the response to soluble growth factors towards proliferation or survival stimuli. These co-operative effects might depend on integrin ability to activate growth factor receptors. In the present paper, we summarize our recent study showing that integrin-dependent adhesion triggers ligand-independent EGFR (epidermal growth factor receptor) activation to transduce downstream signalling. In addition, we also show that integrin-induced signalling pathways are necessary for EGF-dependent transcriptional response, demonstrating the requirement of the ...
TY - JOUR. T1 - Targeted disruption of the epidermal growth factor receptor inhibits development of papillomas and carcinomas from human papillomavirus-immortalized keratinocytes. AU - Woodworth, Craig D.. AU - Gaiotti, Darci. AU - Michael, Evan. AU - Hansen, Laura. AU - Nees, Matthias. PY - 2000/8/15. Y1 - 2000/8/15. N2 - The epidermal growth factor receptor (EGF-R) is frequently overexpressed in human papillomavirus (HPV)-associated dysplasias and carcinomas, implying that it is important for the progression of keratinocytes to malignancy. We used mice with a targeted disruption of the EGF-R gene to directly examine its role in cell immortalization and tumor development. Epidermal keratinocytes were cultured from EGF-R knockout, heterozygous, and wild-type mice, infected with retroviruses encoding HPV-16 E6 and E7 oncogenes, and grafted to nude mice. E6/E7 induced immortalization of EGF-R wild-type cells 5-fold more efficiently than null cells. Immortal EGF-R null cells grew more slowly, ...
TY - JOUR. T1 - A novel mechanism of resistance to epidermal growth factor receptor antagonism in vivo. AU - Rajput, Ashwani. AU - Koterba, Alan P.. AU - Kreisberg, Jeffrey L.. AU - Foster, Jason M.. AU - Willson, James K V. AU - Brattain, Michael G.. PY - 2007/1/15. Y1 - 2007/1/15. N2 - Epidermal growth factor receptor (EGFR) is widely expressed in a number of solid tumors including colorectal cancers. Overexpression of this receptor is one means by which a cell can achieve positive signals for survival and proliferation; another effective means is by constitutive activation of EGFR. We have elucidated the role of constitutive EGFR signaling in malignant progression by stably transfecting colon cancer cells with a human transforming growth factor-α cDNA (a ligand for EGFR) under repressible control by tetracycline. We show that constitutive expression of transforming growth factor-α and its subsequent constitutive activation of EGFR allows for cancer cell survival in response to environmental ...
Epidermal growth factor (EGF) rapidly stimulates receptor autophosphorylation in A-431 cells. After 1 min the phosphorylated receptor can be identified at the plasma membrane using an anti-phosphotyrosine antibody. With further incubation at 37 degrees C, approximately 50% of the phosphorylated EGF receptor was internalized (t1/2 = 5 min) and associated with the tubulovesicular system and later with multivesicular bodies, but not the nucleus. During this period, there was no change in the extent or sites of phosphorylation. At all times the phosphotyrosine remained on the cytoplasmic side of the membrane, opposite to the EGF ligand identified by anti-EGF antibody. These data indicate that (a) the tyrosine-phosphorylated EGF receptor is internalized in its activated form providing a mechanism for translocation of the receptor kinase to substrates in the cell interior; (b) the internalized receptor remains intact for at least 60 min, does not associate with the nucleus, and does not generate any ...
In this study, PU/SF (Antheraea mylitta) scaffolds were fabricated by blending and immobilization techniques. Effective SF dosage was determined and incorporated according to minimum inhibitory concentrations against wound associated bacterial strains while fabricating scaffold. Dermal fibroblast NIH3T3 cells were seeded on epidermal growth factor (EGF) treated and untreated PU/SF scaffolds, fabri...
Epidermal growth factor receptor (EGFR) also known as ErbB1/HER1 is a member of the ErbB family of receptor tyrosine kinases which also includes ErbB2 (Neu, HER2), ErbB3 (HER3) and ErbB4 (HER4). Several ligands such as epidermal growth factor, transforming growth factor-alpha, epigen, amphiregulin, betacellulin, heparin-binding EGF and epiregulin are known to specifically bind to EGFR. Epidermal growth factor (EGF) is one of the high affinity ligands of EGFR. EGF/EGFR system induces growth, differentiation, migration, adhesion and cell survival through various inter-acting signaling pathways. The binding of EGF to the extracellular domain of EGFR induces the dimerization, activation of intrinsic kinase activity and subsequent autophosphorylation of EGFR at multiple residues in the cytoplasmic region such as Tyr 1092, Tyr 1172, Tyr 1197, Tyr 1110, Tyr 1016). Activated EGFR recruits various cytoplasmic proteins which transduce and regulate the EGFR function. The proteins recruited to active EGFR ...
Epidermal growth factor receptor (EGFR) also known as ErbB1/HER1 is a member of the ErbB family of receptor tyrosine kinases which also includes ErbB2 (Neu, HER2), ErbB3 (HER3) and ErbB4 (HER4). Several ligands such as epidermal growth factor, transforming growth factor-alpha, epigen, amphiregulin, betacellulin, heparin-binding EGF and epiregulin are known to specifically bind to EGFR. Epidermal growth factor (EGF) is one of the high affinity ligands of EGFR. EGF/EGFR system induces growth, differentiation, migration, adhesion and cell survival through various inter-acting signaling pathways. The binding of EGF to the extracellular domain of EGFR induces the dimerization, activation of intrinsic kinase activity and subsequent autophosphorylation of EGFR at multiple residues in the cytoplasmic region such as Tyr 1092, Tyr 1172, Tyr 1197, Tyr 1110, Tyr 1016). Activated EGFR recruits various cytoplasmic proteins which transduce and regulate the EGFR function. The proteins recruited to active EGFR ...
The proto-oncogene product, Cbl, is a 120-kDa protein present in lymphocytes that contains numerous PXXP motifs in its COOH-terminal region and constitutively binds the SH3-containing adaptor protein Grb2. Cross-linking of CD3 and CD4 receptors in Jurkat T cells causes tyrosine phosphorylation of Cbl and its association with phosphatidylinositol 3-kinase (Meisner, H., Conway, B., Hartley, D., and Czech, M. P. (1995) Mol. Cell. Biol. 15, 3571-3578). Here we demonstrate that Cbl is also present in nonlymphoid cells, and that epidermal growth factor (EGF) elicits its rapid tyrosine phosphorylation in human embryonic 293 cells. Immunoprecipitates of Cbl from lysates of these cells contain Grb2 in the basal state, while EGF stimulation causes co-precipitation of tyrosine-phosphorylated EGF receptors. Similarly, EGF receptor immunoprecipitates from EGF-treated 293 cells contain Cbl and Grb2. Both Grb2 and EGF receptors are released from Cbl in the presence of a proline-rich peptide that binds the NH2
An epidermal growth factor or EGF is a type of growth factor that controls the proliferation, differentiation and survival of a cell. It is found in all living organisms. The purpose of EGFs is to protect all living tissue by sending out signals for other cells to multiply or release certain chemicals. EGFs are secreted by cells and released into the external environment where they may bind to epidermal growth factor receptors or EGFRs. Upon binding, the EGFs can dimerize with themselves (homodimer) or dimerize with other growth factors (heterodimer). Once dimerized, the inner side of the EGFR that contains a protein kinase adds a phosphoryl group (donated from ATP) to tyrosines on neighboring chains. In doing so, proteins attach to the newly phosphorylated tyrosines thus activating a signaling cascade that ends with DNA synthesis and cell growth.. ...
In spite of intensified efforts to understand cell signaling from endosomes, there is no direct evidence demonstrating that endosomal signaling is sufficient to activate signal transduction pathways and no evidence to demonstrate that endosomal signaling is able to produce a biological outcome. The lack of breakthrough is due in part to the lack of means to generate endosomal signals without plasma membrane signaling. In this paper, we report the establishment of a system to specifically activate epidermal growth factor (EGF) receptor (EGFR) when it endocytoses into endosomes. We treated cells with EGF in the presence of AG-1478, a specific EGFR tyrosine kinase inhibitor, and monensin, which blocks the recycling of EGFR. This treatment led to the internalization of nonactivated EGF-EGFR complexes into endosomes. The endosome-associated EGFR was then activated by removing AG-1478 and monensin. During this procedure we did not observe any surface EGFR phosphorylation. We also achieved specific ...
Epidermal growth factor receptor-dependent mechanisms have been implicated in growth signal transduction pathways that contribute to cancer development, including dermal carcinogenesis. Detection of the extracellular domain of the epidermal growth factor receptor (EGFR ECD) in serum has been suggested as a potential biomarker for monitoring this effect in vivo. Arsenic is a known human carcinogen,
Close, J. L., Liu, J., Gumuscu, B. and Reh, T. A. (2006), Epidermal growth factor receptor expression regulates proliferation in the postnatal rat retina. Glia, 54: 94-104. doi: 10.1002/glia.20361 ...
Supplementary Material for: Role of Epidermal Growth Factor Receptor Expression on Patient Survival in Pancreatic Cancer: A Meta-Analysis
The dual luciferase reporter system was then used to validate the target genes described in Fig 7. To determine whether bta-let-7i and bta-miR-2305 can directly target their predicted target genes, including MAP3K1, proheparin -binding epidermal growth factor-like growth factor (HBEGF), serine/threonine-protein kinase (PAK1) and Ras-related C3 botulinum toxin substrate 2 (RAC2), we designed luciferase reporter constructs that included either the wild- or mutant-type 3-UTR of MAP3K1, HBEGF, PAK1 and RAC2 (Fig 7C). [score:8] ...
HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant mouse HB-EGF produced in E. coli is a single, non-glycosylated polypeptide chain of 86 amino acids (63-148 a.a.) and a molecular mass of 9.8 kDa. It has bee purified by proprietary chromatographic ...
The effects of epidermal growth factor (EGF) on proteins that have been implicated in the migration of the Grade IV brain tumor, glioblastoma multiforme, were investigated in this study. Differential expression levels of TIMP-2, MMP-2, and MT1-MMP in response to EGF treatment were compared by immunoprecipitation, and immunoblotting. This study involved two different cultured glioma cell lines, U87, which expresses wild-type p53, and T98, which has a mutation in the p53 gene, in order to determine if the status of the p53 tumor suppression gene is a factor in the regulation of proteins involved in this migration pathway. It was determined that the only response of mutant cells to EGF treatment was the putative upregulation of the level of TIMP-2 protein. Treatment of the U87 cells resulted in increased phosphorylation of MTl-MMP. It was believed that phosphorylation of MT1-MMP correlated with activity and ability to cleave the proenzyme of MMP-2, however, the level of MMP-2 activity actually decreased.
Epidermal growth factor (EGF)-induced signaling was investigated in cells conditionally defective in clathrin-dependent endocytosis by overexpression of K44A dynamin in HeLa cells and potassium depletion in Hep2 cells. Overexpression of mutant dynamin disrupts high-affinity EGF-EGF receptor (EGFR) i …
The epidermal growth factor (EGF) and its receptor (EGFR) constitute a well-characterized receptor-ligand system that plays an important role in the regulation of cell growth, proliferation, survival, and motility (1-4). EGFR is involved in oncogenic transformation, which can be caused by receptor overexpression, autocrine ligand loops, gene amplification, or activating mutations such as the EGFR type III deletion mutant (EGFRvIII; refs. 5-8).. In a global search for EGFR and EGFRvIII regulated genes, we recently found the mRNA level of the receptor tyrosine kinase EphA2 to be up-regulated by ligand-activated EGFR and the constitutively active variant EGFRvIII (9).. The cDNA sequence of EphA2 (previously known as epithelial cell kinase) was first described by Lindberg and Hunter in 1990 (10). EphA2 belongs to the Eph receptor family, which is the largest subfamily of receptor tyrosine kinases (11). Based on similarities in the extracellular domain sequences, the Eph receptors are divided into ...
HMGB1 (high-mobility group box 1) protein, a pleiotropic cytokine released by several cell types under physiological and pathological conditions, has been identified as a signal molecule active on A431 cells. Although extracellular HMGB1 itself does not trigger any detectable signalling effect on these cells, it induces an increased susceptibility to EGF (epidermal growth factor) stimulation. Specifically, at concentrations of EGF which promote undetectable or limited cell responses, the addition of sub-nanomolar concentrations of HMGB1 potentiates the effect of EGF by specifically activating a downstream pathway that leads to enhanced cell motility through an increase in Ca2+ influx, activation of extracellular-signal-regulated kinase 1/2 and remodelling of the actin cytoskeleton. These results, which identify extracellular HMGB1 as an activator of human tumour cell migration operating in concert with EGF, have important implications in the search for novel strategies to control tumour ...
TY - JOUR. T1 - Ovarian transforming growth factor-β (TGF-β) receptors. T2 - In-vitro effects of follicle stimulating hormone, epidermal growth factor and TGF-β on receptor expression in human preantral follicles. AU - Roy, Shyamal K. AU - Kole, Alok R.. PY - 1998/3/1. Y1 - 1998/3/1. N2 - The expression patterns of transforming growth factor (TGF)-β receptor type I (TβRI) and -II (TβRII) in preand post-menopausal human ovaries, and the in-vitro effects of follicle stimulating hormone (FSH), epidermal growth factor (EGF) and transforming growth factor-β1 (TGF-βI) on receptor expression in preantral follicles were evaluated using immunohistochemistry and immunoblotting. Both types of receptor were present in granulosa, theca and interstitial cells; however, more mural than antral granulosa cells were TβRII positive. Overall, more cells expressed TβRI than TβRII. TβRI and TβRII expressions were detected in the membrane as well as in the soluble fractions. However, marked increases in ...
Background. Epidermal Growth Factor Receptor (EGFR) is a key target molecule in current treatment of several neoplastic diseases. Hence, in order to develop and improve current drugs targeting EGFR signalling, an accurate understanding of how this signalling pathway is regulated is required. It has recently been demonstrated that inhibition of cAMP-dependent protein kinase (PKA) induces a ligand-independent internalization of EGFR. Cyclic-AMP-dependent protein kinase consists of a regulatory dimer bound to two catalytic subunits.. Results. We have investigated the effect on EGFR levels after ablating the two catalytic subunits, Cα and Cβ in two different models. The first model used targeted disruption of either Cα or Cβ in mice whereas the second model used Cα and Cβ RNA interference in HeLa cells. In both models we observed a significant reduction of EGFR expression at the protein but not mRNA level.. Conclusion. Our results suggest that PKA may represent a target that when manipulated ...
BACKGROUND INFORMATION: ARAP1 is an Arf (ADP-ribosylation factor)-directed GAP (GTPase-activating protein) that inhibits the trafficking of EGFR (epidermal growth factor receptor) to the early endosome. To further understand the function of ARAP1, we sought to identify proteins that interact with ARAP1. RESULTS: Here we report that ARAP1 associates with the CIN85 (Cbl-interacting protein of 85 kDa). Arg86 and Arg90 of ARAP1 and the SH3 (Src homology 3) domains of CIN85 are necessary for the interaction. We found that a mutant of ARAP1 with reduced affinity for CIN85 does not efficiently rescue the effect of reduced ARAP1 expression on EGFR trafficking to the early endosome. Reduced expression of CIN85 has a similar effect as reduced expression of ARAP1 on traffic of the EGFR. Cbl proteins regulate the endocytic trafficking of the EGFR by mediating ubiquitination of the EGFR. Overexpression of ARAP1 reduced ubiquitination of the EGFR by Cbl and slowed Cbl-dependent degradation of the EGFR. ...
TY - JOUR. T1 - Cyclooxygenase-2 (COX-2) negatively regulates expression of epidermal growth factor receptor and causes resistance to gefitinib in COX-2-overexpressing cancer cells. AU - Kim, Young-Mee. AU - Park, Soo Yeon. AU - Pyo, Hongryull. PY - 2009/8/1. Y1 - 2009/8/1. N2 - Overexpression of cyclooxygenase-2 (COX-2) and epidermal growthfactor receptor (EGFR) has been detected in many types of cancer. Although COX-2 and EGFR are closely related to each other, the exact mechanism of COX-2 in tumors has not been well understood. In this study, we investigated the relationship between COX-2 and EGFR in cancer cells. Using two cell lines stably overexpressing COX-2 (HCT-116-COX-2 and H460-COX-2) and a stable line of COX-2 knockdown MOR-P cells, we analyzed patterns of COX-2 and EGFR expression. To observe the effects of COX-2 on EGFR expression and activity, we did comparative analyses after treatment withvarious drugs (EGF, celecoxib, prostaglandin E2, gefitinib, Ro-31-8425, PD98059, and ...
FSAP (Factor VII-activating protease) is a novel plasma-derived serine protease that regulates haemostasis as well as vascular cell proliferation. FSAP undergoes autoactivation in the presence of polyanionic macromolecules such as heparin and RNA. Competition experiments suggest that RNA and heparin bind to the same or overlapping interaction sites. A proteolysis approach, where FSAP was hydrolysed into smaller fragments, was used to identify the polyanion-binding site. The EGF (epidermal growth factor)-like domains EGF2 and EGF3 of FSAP are the major interaction domains for RNA. The amino acids Arg170, Arg171, Ser172 and Lys173 within the EGF3 domain were essential for this binding. This is also the region with the highest positive net charge in the protein and is most probably located in an exposed loop. It is also highly conserved across five species. Disruption of disulphide bridges led to the loss of RNA and heparin binding, indicating that the three-dimensional structure of the EGF3 domain ...
A DNA fragment distinct from the epidermal growth factor receptor (EGF-R) and erbB-2 genes was detected by reduced stringency hybridization of v-erbB to normal genomic human DNA. Characterization of the cloned DNA fragment mapped the region of v-erbB homology to three exons with closest homology of 64 % and 67 % to a contiguous region within the tyrosine kinase domains of the EGF-R and erbB-2 proteins, respectively, cDNA cloning revealed a predicted 148 kd transmembrane polypeptide with structural features identifying it as a member of the erbB family, prompting designation of the new gene as erbB-3. It was mapped to human chromosome 12q11-13 and was shown to be expressed as 6.2 kb transcript in a variety of normal tissues of epithelial origin. Markedly elevated erbB-3 mRNA levels were demonstrated in certain human mammary tumor cell lines. These findings indicate that increased erbB-3 expression, as in the case of EGF-R and erbB-2, plays a role in some human malignancies.
Mycoplasma pneumoniae (M. pneumoniae) is increasingly recognized as a major cause of acute respiratory tract infections. Today, macrolides are used in the primary treatment of M. pneumoniae infection. However, with the increasing prevalence of strains resistant to macrolides, as well as reports of toxicity and adverse side effects, it is necessary to develop an alternative therapeutic agent. A compound recipe - Qinbaiqingfei pellets (Qinbai) - have already been approved in China as the first effective traditional Chinese medicine to be used against M. pneumoniae. Herein, we characterize the mechanism by which Qinbai interacts with M. pneumoniae and lung epithelial cells. The fact that Baicalin is the key component of Qingbai leads us to believe its study is important to elucidating the mechanism of the action of Qinbai. In this study, we describe the complex impact of Baicalin on the adhesin protein P1 of M. pneumoniae and on the expression of epidermal growth factor (EGF) in BALB/c mice and A549 cells
Overexpression of human epidermal growth factor receptor 2 (HER2) is associated with tumor aggressiveness and poor prognosis in breast cancer. With the availability of therapeutic antibodies against HER2, great strides have been made in the clinical management of HER2 overexpressing breast cancer. However, de novo and acquired resistance to these antibodies presents a serious limitation to successful HER2 targeting treatment. The identification of novel epitopes of HER2 that can be used for functional/region-specific blockade could represent a central step in the development of new clinically relevant anti-HER2 antibodies. In the present study, we present a novel computational approach as an auxiliary tool for identification of novel HER2 epitopes. We hypothesized that the structurally and linearly evolutionarily conserved motifs of the extracellular domain of HER2 (ECD HER2) contain potential druggable epitopes/targets. We employed the PROSITE Scan to detect structurally conserved motifs and ...
Epidermal growth factor receptor[edit]. An example of RTKs that undergo autophosphorylation is the Epidermal Growth Factor ... 1: Activation of the Epidermal Growth Factor Receptor by autophosphorylation. Adapted from Pecorino (2008)[1] ... In this way, src-kinase deregulation can enhance tumor growth and invasive potential of cancer cells.[2] The activity of src ... Src kinases are involved in intracellular signaling pathways that influence cell growth and cell adhesion strength. The latter ...
... which was renamed epidermal growth factor. He continued research on cellular growth factors after joining the faculty of ... Prize in Physiology or Medicine in 1986 for the isolation of nerve growth factor and the discovery of epidermal growth factor. ... Prize in Physiology or Medicine in 1986 for the isolation of nerve growth factor and the discovery of epidermal growth factor ... Cohen, Stanley (1993). "Epidermal Growth Factor" (PDF). In Tore Frängsmyr; Jan Lindsten (eds.). Nobel Lectures, Physiology or ...
Epidermal growth factor. Transforming growth factor alpha. Cholera toxin. Within the epidermis keratinocytes are associated ... The roles of transforming growth factor-alpha and epidermal growth factor". Cell. 50 (7): 1131-7. doi:10.1016/0092-8674(87) ... Rheinwald, JG; Green, H (1977). "Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes". ... These factors include: The transcription factor p63, which prevents epidermal stem cells from differentiating into ...
... epidermal growth factor; EGFR, epidermal growth factor receptor; MoAbs, monoclonal antibodies; VEGF, vascular endothelial ... growth factor. The major challenge in the development of boron delivery agents has been the requirement for selective tumor ... Ono, Koji (28 March 2016). "An analysis of the structure of the compound biological effectiveness factor". Journal of Radiation ... In vivo growth suppression of experimental melanoma solid tumor". Cancer Letters. 150 (2): 177-82. doi:10.1016/S0304-3835(99) ...
Epidermal growth factor (EGF). *Various enzymes; there are three major enzymes found in saliva: *α-amylase (EC3.2.1.1), or ... Herbst RS (2004). "Review of epidermal growth factor receptor biology". International Journal of Radiation Oncology, Biology, ... Researchers at the University of Florida at Gainesville have discovered a protein called nerve growth factor (NGF) in the ... stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids ...
They are serine-threonine kinases that contain epidermal growth factor (EGF) repeats, a cytoplasmic kinase and are located in ... Sampoli Benitez, Benedetta A; Komives, Elizabeth A (2000). "Disulfide bond plasticity in epidermal growth factor". Proteins: ... bound to the cell wall and contains a series of epidermal growth factor repeats. WAKs are found in various plants and crops ... all of which contain at least two epidermal growth factor (EGF). Of all these isoforms, WAK1 and WAK2 are most ubiquitous and ...
Vertebrate hepatocyte growth factor-regulated tyrosine kinase substrate (HRS). Mammalian epidermal growth factor receptor ... Mammalian epidermal growth factor receptor substrate EPS15R. Drosophila melanogaster (Fruit fly) liquid facets (lqf), an epsin ... substrate 15 (EPS15), which is involved in cell growth regulation. ...
October 2003). "Aldosterone stimulates epidermal growth factor receptor expression". J. Biol. Chem. 278 (44): 43060-66. doi: ... and also by epidermal growth factor (EGF), which is a target of the signaling pathway activated by aldosterone Reduced ... Many of these remodelling effects seem to be mediated by transforming growth factor beta (TGF-beta), which is a common ...
Effects of EGF Epidermal growth factor (EGF) results in cellular proliferation, differentiation, and survival. EGF is a low- ... Herbst RS (2004). "Review of epidermal growth factor receptor biology". International Journal of Radiation Oncology, Biology, ... Additional cells present include parietal cells that secrete hydrochloric acid and intrinsic factor, chief cells that secrete ... The parietal cells of the human stomach are responsible for producing intrinsic factor, which is necessary for the absorption ...
... growth factor receptor antibodies which are inhibitors of epidermal growth factor binding and antagonists of epidermal growth ... "Targeting the epidermal growth factor receptor for cancer therapy". Journal of Clinical Oncology. Retrieved March 2, 2011. " ... "Epidermal growth factor receptor family and chemosensitization". Journal of the National Cancer Institute. Retrieved March 2, ... Antibody 225 (commercially known as Cetuximab, or Erbitux) against the receptor for epidermal growth factor was approved by the ...
... including epidermal growth factor receptor (EGFR; ERBB1), epidermal growth factor receptor 2 (HER2; ERBB2), vascular ... June 2007). "Inhibition of the T790M gatekeeper mutant of the epidermal growth factor receptor by EXEL-7647". Clinical Cancer ... June 2007). "Inhibition of the T790M gatekeeper mutant of the epidermal growth factor receptor by EXEL-7647". Clinical Cancer ... endothelial growth factor receptor (VEGFR), and ephrin B4 (EphB4). The drug activity was initially studied in non-small cell ...
... and interaction partners of epidermal growth factor receptor signaling after stimulation by epidermal growth factor using ... "Crystal structure of a truncated epidermal growth factor receptor extracellular domain bound to transforming growth factor α". ... "Crystal structure of a truncated epidermal growth factor receptor extracellular domain bound to transforming growth factor ... There are 11 growth factors that activate ErbB receptors. The ability ('+') or inability ('-') of each growth factor to ...
Wong ES, Fong CW, Lim J, Yusoff P, Low BC, Langdon WY, Guy GR (September 2002). "Sprouty2 attenuates epidermal growth factor ... "Ligand-induced ubiquitination of the epidermal growth factor receptor involves the interaction of the c-Cbl RING finger and ... "cbl-b inhibits epidermal growth factor receptor signaling". Oncogene. 18 (10): 1855-66. doi:10.1038/sj.onc.1202499. PMID ... Sehat B, Andersson S, Girnita L, Larsson O (July 2008). "Identification of c-Cbl as a new ligase for insulin-like growth factor ...
Appella E, Weber IT, Blasi F (April 1988). "Structure and function of epidermal growth factor-like regions in proteins". FEBS ... Davis CG (May 1990). "The many faces of epidermal growth factor repeats". New Biol. 2 (5): 410-9. PMID 2288911. Blomquist MC, ... Doolittle RF, Feng DF, Johnson MS (1984). "Computer-based characterization of epidermal growth factor precursor". Nature. 307 ( ... "How an epidermal growth factor (EGF)-like domain binds calcium. High resolution NMR structure of the calcium form of the NH2- ...
Harari, P M (December 2004). "Epidermal growth factor receptor inhibition strategies in oncology". Endocrine-Related Cancer. 11 ... September 2006). "Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their ...
"The solution structure of human epidermal growth factor". Nature. 327 (6120): 339-341. Bibcode:1987Natur.327..339C. doi:10.1038 ...
CBLB has been shown to interact with: CRKL, Epidermal growth factor receptor, Grb2, NEDD4, PIK3R1, and SH3KBP1. GRCh38: Ensembl ... 2001). "Cbl-b-dependent coordinated degradation of the epidermal growth factor receptor signaling complex". J. Biol. Chem. 276 ... 1999). "cbl-b inhibits epidermal growth factor receptor signaling". Oncogene. 18 (10): 1855-66. doi:10.1038/sj.onc.1202499. ... "cbl-b inhibits epidermal growth factor receptor signaling". Oncogene. 18 (10): 1855-66. doi:10.1038/sj.onc.1202499. PMID ...
Colony stimulating factor 1 receptor, DCTN1, DNM1, Dock180, Dystroglycan, EPH receptor A2, ETV6, Epidermal growth factor ... Grb2 is best known for its ability to link the epidermal growth factor receptor tyrosine kinase to the activation of Ras and ... The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and ... Kato-Stankiewicz J, Ueda S, Kataoka T, Kaziro Y, Satoh T (June 2001). "Epidermal growth factor stimulation of the ACK1/Dbl ...
Inhibitors of Epidermal growth factor receptor (EGFR) *tyrosine kinase inhibitors (TKI's):[9] *erlotinib (Tarceva)[10][ ... Riely GJ, Politi KA, Miller VA, Pao W (December 2006). "Update on epidermal growth factor receptor mutations in non-small cell ... Tatematsu A, Shimizu J, Murakami Y, Horio Y, Nakamura S, Hida T, Mitsudomi T, Yatabe Y (October 2008). "Epidermal growth factor ... Shigematsu H, Gazdar AF (January 2006). "Somatic mutations of epidermal growth factor receptor signaling pathway in lung ...
October 2003). "Aldosterone stimulates epidermal growth factor receptor expression". J. Biol. Chem. 278 (44): 43060-66. doi: ... and also by epidermal growth factor (EGF), which is a target of the signaling pathway activated by aldosterone[35] ... Many of these remodelling effects seem to be mediated by transforming growth factor beta (TGF-beta), which is a common ... April 2008). "Factors Identified as Precipitating Hospital Admissions for Heart Failure and Clinical Outcomes: Findings From ...
Beyond this, exons 2-6 code the ligand binding region; 7-14 code the epidermal growth factor (EGF) domain; 15 codes the ... There are three "growth factor" repeats; A, B and C. A and B are closely linked while C is separated by the YWTD repeat region ...
2002). "Sprouty2 attenuates epidermal growth factor receptor ubiquitylation and endocytosis, and consequently enhances Ras/ERK ... 1999). "Cbl-b inhibits epidermal growth factor receptor signaling". Oncogene. 18 (10): 1855-66. doi:10.1038/sj.onc.1202499. ... CBLC has been shown to interact with FYN and Epidermal growth factor receptor. GRCh38: Ensembl release 89: ENSG00000142273 - ... 2002). "C-Cbl is involved in Met signaling in B cells and mediates hepatocyte growth factor-induced receptor ubiquitination". J ...
Epidermal growth factor (EGF) binds to EGF receptor, also known as HER-1 or ErbB1, to initiate transmembrane signaling. Lipid ... Mineo, Chieko; Gill, Gordon N.; Anderson, Richard G.W. (1999). "Regulated migration of epidermal growth factor receptor from ... Roepstorff, Kirstine; Thomsen, Peter; Sandvig, Kirsten; van Deurs, Deurs (2002). "Sequestration of epidermal growth factor ... Miljan, Erik A; Bremer, Eric G. (2002). "Regulation of growth factor receptors by gangliosides". Sci STKE. 2002 (160): RE15. ...
Hollenberg, MD (1979). Epidermal growth factor-urogastrone, a polypeptide acquiring hormonal status. Vitamins & Hormones. 37. ... Another important factor in the rate of ligation reaction is the pH dependence which corresponds to a pKa of 5.6, which is not ... a factor in the cleavage reaction . This particular dependence requires a protonated base at position A756 of the ribozyme. ...
It binds to the epidermal growth factor receptor (EGFR). The US FDA approved necitumumab under the brand name Portrazza for use ...
Mutations in RHBDF2 inhibit tumour necrosis factor alpha. RHBDL2 also acts on Epidermal growth factor and EphrinB3. ... RHBDF2 is involved in the regulation of the secretion of several ligands of the epidermal growth factor receptor. The rhomboid ... Analysis of a Finnish family confirms RHBDF2 mutations as the underlying factor in tylosis with esophageal cancer. Fam Cancer ... Human rhomboid family-1 gene RHBDF1 participates in GPCR-mediated transactivation of EGFR growth signals in head and neck ...
... "lDirect Stimulation of Bone Resorption by Epidermal Growth Factor*". Endocrinology. 107 (1): 270-273. doi:10.1210/endo-107-1- ...
Expression of functionally active human epidermal growth factor has been done in C. glutamicum, thus demonstrating a potential ... Date M, Itaya H, Matsui H, Kikuchi Y (January 2006). "Secretion of human epidermal growth factor by Corynebacterium glutamicum ... The expression system C1 shows a low viscosity morphology in submerged culture, enabling the use of complex growth and ...
Expression of functionally active human epidermal growth factor has been brought about in C. glutamicum, thus demonstrating a ... OCLC 826400572.[page needed] Date, M.; Itaya, H.; Matsui, H.; Kikuchi, Y. (2006). "Secretion of human epidermal growth factor ... and other nutritional factors (Martín, 1989); bioconversion of steroids; degradation of hydrocarbons; cheese aging; and ... Database of Corynebacterial Transcription Factors and Regulatory Networks Rollins, David M. University of Maryland: Pathogentic ...
... when their growth is limited by the available light.[16] Other factors include the need to balance water loss at high ... The epidermis tissue includes several differentiated cell types; epidermal cells, epidermal hair cells (trichomes), cells in ... The epidermal cells are the most numerous, largest, and least specialized and form the majority of the epidermis. They are ... Once sugar has been synthesized, it needs to be transported to areas of active growth such as the plant shoots and roots. ...
High levels of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) are also associated with worsened acne.[42] Both ... These peels only affect the epidermal layer of the skin and can be useful in the treatment of superficial acne scars as well as ... Another common factor is the excessive growth of the bacterium Cutibacterium acnes, which is present on the skin.[15] ... These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ...
... and epidermal growth factor. Because these regions are related to complexed signal transduction pathways mediated by cytokines ... Koon HW, Zhao D, Na X, Moyer MP, Pothoulakis C (Oct 2004). "Metalloproteinases and transforming growth factor-alpha mediate ... "Neurotrophic and anhidrotic keratopathy treated with substance P and insulinlike growth factor 1". Archives of Ophthalmology. ... Cell growth, proliferation, angiogenesis, and migrationEdit. The above processes are part and parcel to tissue integrity and ...
It is mainly used to treat cases of NSCLC that harbour mutations in the epidermal growth factor receptor (EGFR) gene.[5] ... Afatinib covalently binds to cysteine number 797 of the epidermal growth factor receptor (EGFR) via a Michael addition (IC50 = ... Phase II results for breast cancer that over-expresses the protein human epidermal growth factor receptor 2 (Her2-positive ... and epidermal growth factor receptor (EGFR) kinases. Afatinib is not only active against EGFR mutations targeted by first ...
"Microfluidics-assisted fluorescence in situ hybridization for advantageous human epidermal growth factor receptor 2 assessment ... Fluorescent signal strength depends on many factors such as probe labeling efficiency, the type of probe, and the type of dye. ...
regulation of epidermal growth factor-activated receptor activity. • regulation of resting membrane potential. • regulation of ... negative regulation of epidermal growth factor-activated receptor activity. • cell adhesion. • hematopoietic progenitor cell ... growth cone. • neuromuscular junction. • intracellular. • protein complex. • axon. • nuclear outer membrane. • endoplasmic ... Because of the development to the resistance to chemical, MDR cells become a critical factor on the success of cancer ...
Stacey M, Lin HH, Hilyard KL, Gordon S, McKnight AJ (2001). „Human epidermal growth factor (EGF) module-containing mucin-like ...
Nimotuzumab is an inhibitor of epidermal growth factor receptor (EGFR), which is over-expressed in many cancers. Nimotuzumab is ... "The two determining factors underlying the crisis are well known. One is the dissolution of the Soviet Union and the socialist ... There are other factors beside the embargo explaining the lack of imports, in particular Cuba's lack of hard currency. Those ... Here, students are exposed to medicine and the social, economical, and political factors that influence health.[52] At primary ...
... es that cause tumor growth include Rous sarcoma virus and Mouse mammary tumor virus. Cancer can be triggered by proto ... "Cell-to-cell transmission of retroviruses: Innate immunity and interferon-induced restriction factors". Virology. 411 (2): 251 ... Walleye epidermal hyperplasia virus. Gammaretrovirus. *Murine leukemia virus. *Abelson murine leukemia virus ...
Risk factors for skeletal effects include older age, greater dosage and longer course of treatment. Most bone changes cause no ... Isotretinoin may stop long bone growth in young people who are still growing.[7] Premature epiphyseal closure can occur in ... While excessive bone growth has been raised a possible side effect, a 2006 review found little evidence for this.[84] ... excessive bone growth), especially in growing children and adolescents.[32] Other problems include premature epiphyseal closure ...
For example, despite widespread expression of epidermal growth factor receptors (EGFRs) and EGF family ligands in non-small- ... One approach used by tumors to upregulate growth and survival is through autocrine production of growth and survival factors. ... alterations that lead to de-regulation of the autocrine Wnt pathway result in transactivation of epidermal growth factor ... Gene expression profiling revealed the prevalence of specific fibroblast growth factors (FGFs) and FGF receptors in NSCLC cell ...
Key factors that affect seasonal reproduction include the length of the wet season, subsequent food availability, and the ... This involves smearing secretions from epidermal scent glands on tree branches, along with urine and feces. In some cases, ... fetal growth rates are generally slower in strepsirrhines, which results in newborn offspring that are as little as one-third ... depending on factors such as temperature and predation.[125] Many extant strepsirrhines are well adapted for nocturnal activity ...
... phase III trial of chemotherapy with or without bevacizumab for first-line treatment of human epidermal growth factor receptor ... Anna Dorothea Wagner, Christoph Thomssen, Johannes Haerting, Susanne Unverzagt: Vascular-endothelial-growth-factor (VEGF) ... Quantifying antivascular effects of monoclonal antibodies to vascular endothelial growth factor: insights from imaging. In: ... Vascular Endothelial Growth Factor) ein.[4]. Bevacizumab ist ein Angiogenesehemmer, der zielgerichtet an den Wachstumsfaktor ...
... as he shared the award with Rita Levi-Montalcini of Italy for their discovery of epidermal growth factor, a hormone that can ... VUMC's split ushers in 'bright' era of change, growth. Tennesseean, April 30, 2016. ...
... comparing PDGF to epidermal growth factor (EGF), which is also implicated in stimulating cell growth, proliferation, and ... Platelet-derived growth factor (PDGF) is one among numerous growth factors that regulate cell growth and division. In ... "Vascular endothelial growth factor B, a novel growth factor for endothelial cells". Proc. Natl. Acad. Sci. U.S.A. 93 (6): 2567- ... Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)[16][17] which are ...
"Wiskott-Aldrich syndrome protein is associated with the adapter protein Grb2 and the epidermal growth factor receptor in living ... MBInfo - WASP and other Nucleation Promotion Factors. *GeneReviews/NIH/NCBI/UW entry on WAS-Related Disorders including Wiskott ... Rajmohan R, Meng L, Yu S, Thanabalu T (April 2006). "WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta ... "Activation of Wiskott-Aldrich syndrome protein and its association with other proteins by stromal cell-derived factor-1alpha is ...
... α and epidermal growth factor during fetal and neonatal development published in 1992 and 1993. There, she continued to ... featured her work on insulin-related growth factors. The segment ran as part of a six-part PBS series on women in science, ... The laboratory also discovered direct evidence that the Gap-43 protein was important in the growth of the axons of nerve cells. ... School with a lighter teaching workload and more research opportunities including her research on transforming growth factor- ...
He considered social factors such as poverty major causes of disease.[103] He even attacked Koch's and Ignaz Semmelweis' policy ... They found that the larynx was extensively damaged due to ulcer, and microscopic examination confirmed epidermal carcinoma. Die ... Virchow's Law, during craniosynostosis, skull growth is restricted to a plane perpendicular to the affected, prematurely fused ... Related to this research, Virchow described the factors contributing to venous thrombosis, Virchow's triad.[22][58] ...
Risk factors for abscess formation include intravenous drug use.[16] Another possible risk factor is a prior history of disc ... Five-day-old inflamed epidermal inclusion cyst. The black spot is a keratin plug which connects with the underlying cyst. ... Skin abscesses are common and have become more common in recent years.[1] Risk factors include intravenous drug use with rates ... Skin abscesses are common and have become more common in recent years.[1] Risk factors include intravenous drug use with rates ...
"Membrane-anchored growth factors, the epidermal growth factor family: Beyond receptor ligands". Cancer Science 99 (2): 214-20. ...
High levels of prolactin during pregnancy and breastfeeding also increase insulin resistance, increase growth factor levels ( ... Chong, K.; Joshi, S.; Jin, L. T.; Shu-Chien, A. C. (2006). "Proteomics profiling of epidermal mucus secretion of a cichlid ( ... prolactin and growth hormone production, are essential. Growth hormone (GH) is structurally very similar to prolactin and ... This hormone is closely associated with prolactin and appears to be instrumental in breast, nipple, and areola growth before ...
... characterization of mutations of the epidermal growth factor receptor gene in human lung cancers, including a common mutation ...
Epidermal growth factor. *Journal of Peptide Science. *Lactotripeptides. *Multifunctional peptides. *Neuropeptides. *Palmitoyl ... The Australian Crime Commission cited the alleged misuse of secretagogue peptides in Australian sport including growth hormone ...
Recent studies demonstrated the involvement of growth factors, such as epidermal growth factor (EGF) and neurotensin in the 5- ... The PI3k/Akt signaling cascade works with the transforming growth factor beta/SMAD signaling cascade to ensure prostate cancer ... Risk factors. A complete understanding of the causes of prostate cancer remains elusive.[17] The primary risk factors are ... Factors that increase the risk of prostate cancer include: older age, a family history of the disease, and race.[3] About 99% ...
... or insulin-like growth factor 2 Duodenal cancer List of cutaneous conditions Thiers BH, Sahn RE, Callen JP (2009). "Cutaneous ... The histopathologic features of NME are nonspecific and include: epidermal necrosis subcorneal pustules confluent parakeratosis ... epidermal hyperplasia, and marked papillary dermal hyperplasia in a psoriasiform pattern angioplasia of papillary dermis ... suppurative folliculitis The vacuolated, pale, swollen epidermal cells and necrosis of the superficial epidermis are most ...
Growth factors. *Epidermal growth factor. *Fibroblast growth factor. *Nerve growth factor. *Platelet-derived growth factor ... Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... Hepatoma-derived growth factor (HDGF). *Interleukins/T-cell growth factors (see here instead) ...
Epidermal growth factor. *Insulin-like growth factor 1. *Epithelial-mesenchymal transition. *Madin-Darby Canine Kidney Cells ... Hepatocyte growth factor (HGF) or scatter factor (SF) is a paracrine cellular growth, motility and morphogenic factor. It is ... Nakamura T (1992). "Structure and function of hepatocyte growth factor". Progress in Growth Factor Research. 3 (1): 67-85. doi: ... "Hepatocyte Growth Factor Mediates Enhanced Wound Healing Responses and Resistance to Transforming Growth Factor-β₁-Driven ...
... epidermal growth factor (EGF), IGF-1, and fibroblast growth factor (FGF), which in turn have specific roles in breast growth ... "Mitogenic properties of insulin-like growth factors I and II, insulin-like growth factor binding protein-3 and epidermal growth ... tumor necrosis factor β (TNF-β), transforming growth factor α (TGF-α),[67] transforming growth factor β (TGF-β),[68] heregulin, ... "Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial ...
A] couple of factors - the high taxes the federal government imposed on growing hemp in the late 1930s and again in the early ' ... George Washington pushed for the growth of hemp and even grew hemp himself, as it was a cash crop commonly used to make rope ... secreted in a resinous mixture by epidermal hairs called glandular trichomes, although they can also be distinguished ... In 2011, the U.S. imported $11.5 million worth of hemp products, mostly driven by growth in the demand for hemp seed and hemp ...
Amelioration of experimental arthritis by intra-articular injection of an epidermal growth factor receptor tyrosine kinase ... Abundant levels of epidermal growth factor receptor (EGFR) are expressed in the synovial lining layers, and the anti-arthritis ...
Neurotrophic actions of a novel molluscan epidermal growth factor. P.M. Hermann, R.E. van Kesteren, W.C. Wildering, S.D. ... Neurotrophic actions of a novel molluscan epidermal growth factor. / Hermann, P.M.; van Kesteren, R.E.; Wildering, W.C.; ... title = "Neurotrophic actions of a novel molluscan epidermal growth factor",. author = "P.M. Hermann and {van Kesteren}, R.E. ... Neurotrophic actions of a novel molluscan epidermal growth factor. The Journal of Neuroscience. 2000;20(17):6355-6364. ...
Epidermal growth factors in the kidney and relationship to hypertension. * Association between epidermal growth factor gene ... Effectiveness of Epidermal Growth Factor-containing Ointment on the Solar Lentigines. *Endometrial Heparin-binding Epidermal ... Growth Factor Expression. *Efficacy and Safety Evaluation of the Intralesional Recombinant Human Epidermal Growth Factor (rhEGF ... Epidermal Growth Factor Receptor Mutations in the Blood of Patients With Advanced Non-Small Cell Lun… ...
Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis ...
Epidermal growth factor induces the functional expression of dopamine receptors in the GH3 cell line. In: Endocrinology. 1991 ... Epidermal growth factor induces the functional expression of dopamine receptors in the GH3 cell line. / Missale, Cristina; ... title = "Epidermal growth factor induces the functional expression of dopamine receptors in the GH3 cell line", ... Epidermal growth factor induces the functional expression of dopamine receptors in the GH3 cell line. ...
Mutations in the epidermal growth factor receptor (EGFR) are observed in a fraction of non-small-cell lung cancers (NSCLS). ... N2 - Mutations in the epidermal growth factor receptor (EGFR) are observed in a fraction of non-small-cell lung cancers (NSCLS ... AB - Mutations in the epidermal growth factor receptor (EGFR) are observed in a fraction of non-small-cell lung cancers (NSCLS ... abstract = "Mutations in the epidermal growth factor receptor (EGFR) are observed in a fraction of non-small-cell lung cancers ...
Epidermal growth factor receptor (EGFR) is a major oncogenic driver in glioblastoma (GBM) without mutations in the isocitrate ...
... the intralesional administration of human recombinant epidermal growth factor (hrEGF) has been approved for the treatment of ... From: Active post-marketing surveillance of the intralesional administration of human recombinant epidermal growth factor in ...
Return to Article Details A controlled, efficient and robust process for the synthesis of an epidermal growth factor receptor ...
Epidermal growth factor (EGF) has been shown to have a mitogenic effect on some breast cancer cells lines in vitro. The growth ... Relationship of the epidermal growth factor-receptor to the growth fraction (Ki-67 antibody) and the flow cytometric S-phase as ... Relationship of the epidermal growth factor-receptor to the growth fraction (Ki-67 antibody) and the flow cytometric S-phase as ... Dive into the research topics of Relationship of the epidermal growth factor-receptor to the growth fraction (Ki-67 antibody) ...
EGFR: epidermal growth factor receptor. EPA: U.S. Environmental Protection Agency. ER: estrogen receptor ... The committee was also asked to comment on the usefulness of toxic equivalency factors (TEFs) and the uncertainties associated ... Use of Toxic Equivalency Factors for Risk Estimation of DLCs and Mixture of DLCs, ... the validity of the nonthreshold linear dose-response model and the cancer slope factor calculated by EPA through the use of ...
Targeting the epidermal growth factor receptor (EGFR) - a new therapeutic option in oncology?. Swiss Med Wkly. 2006;136(01-02): ...
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor ... HER2-positive breast cancer is a breast cancer that tests positive for a protein called human epidermal growth factor receptor ... 2 (HER2), which promotes the growth of cancer cells. In about 1 of every 5 breast cancers, the cancer cells have a gene ... Epidermal growth factor receptor. ...
Also searched for Human epidermal growth factor receptor 2 and Erbb2. See Search Details ...
epidermal growth factor receptor. neutrophil count. epidermal growth factor. potassium. human papillomavirus ...
And lately EGF, epidermal growth factor, has been getting me excited. It really helps rejuvenate. Its not going to make you ...
Everything from Epidermal Growth Factors, Retinol, Hyaluronic, Vitamin C and more. Customize the kit to your skin type. Simple ...
Kevin M. Kalinsky, MD, MS: There have been some real advances in HER2 [human epidermal growth factor receptor 2]-positive ...
The antibody used in this study is called anti-HER2 (Human Epidermal Growth Factor Receptor 2). This antibody sticks to HER2- ...
ENHERTU is a prescription medicine used in adults to treat human epidermal growth factor receptor 2 (HER2)-positive stomach ... or human epidermal growth factor receptor 2, positive. HER2 is a protein found on the surface of cells. While many types of ...
Does the epidermal growth factor receptor mediate airway epithelial repair following a single chlorine exposure in mice? ...
The Icelandic brands signature plant-derived epidermal growth factor (EGF) won its discoverers a Nobel Prize for medicine back ... Considered an age-preventing power-protein, it supports cell renewal and wound repair while stimulating collagen growth. The ... stimulate healthy new skin-cell growth with a V8 Peptide Complex, and hydrate the complexion all at once. Leave it on as a mask ...
Epidermal Growth Factor Receptor (EGFR) Tyrosine Kinase Inhibitor. Iressa®. Cancer-related. Gefitinib - What is it for. ...
Everything You Need To Know About Epidermal Growth Factor (EGF) Move over retinols, theres a new It ingredient rising up the ... skincare ranks and its called Epidermal Growth Factor - or EGF for short. The go-to for dermatologists thanks to its collagen- ... At its core, Salicylic Acid helps clear pores and promote new cell growth. By doing so, youre able to treat current blemishes ...
... a new phase I/II study of a first-in-class photoimmunotherapy for solid tumors that overexpress the epidermal growth factor ...
2. Have NSCLC with epidermal growth factor receptor or anaplastic lymphoma kinase genomic tumor aberrations; 3. Have ...
... has recently been shown to significantly suppress the growth and survival of GBM through inhibiting epidermal growth factor ... Although the growth of the tumor cells in a relatively closed space may partially account for its malignancy, highly invasive ...
Spatial activation of ezrin by epidermal growth factor receptor and focal adhesion kinase co-ordinates epithelial cell ... but there is a gradual recovery in type VII collagen secretion from basal keratinocytes to the dermal-epidermal junction, with ... but there is a gradual recovery in type VII collagen secretion from basal keratinocytes to the dermal-epidermal junction, with ... but there is a gradual recovery in type VII collagen secretion from basal keratinocytes to the dermal-epidermal junction, with ...
ObjectivesTo examine combined immunoprofiles of epidermal growth factor receptor (EGFR), CD44, and p16 in oropharyngeal ...
  • Epidermal growth factor ( EGF ) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR . (wikipedia.org)
  • EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface . (wikipedia.org)
  • Eps15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor (EGFR). (nih.gov)
  • The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). (wikipedia.org)
  • Interruption of EGFR signalling, either by blocking EGFR binding sites on the extracellular domain of the receptor or by inhibiting intracellular tyrosine kinase activity, can prevent the growth of EGFR-expressing tumours and improve the patient's condition. (wikipedia.org)
  • Epidermal growth factor receptor (EGFR) is a transmembrane protein that is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα) ErbB2 has no known direct activating ligand, and may be in an activated state constitutively or become active upon heterodimerization with other family members such as EGFR. (wikipedia.org)
  • Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer. (wikipedia.org)
  • EGFR signaling-dependent inhibition of glioblastoma growth by ginsenoside Rh2. (greenmedinfo.com)
  • Thefamily consists of epidermal growth factor receptor (EGFR), HER2, HER3, andHER4, and at least 10 ligands that bind and activate family members. (cancernetwork.com)
  • 3]Preclinical and clinical data support the involvement of the ligands'transforming growth factor-alpha and epidermal growth factor and EGFR in theformation and progression of human cancers. (cancernetwork.com)
  • EGFR expression levels in the premalignant lesion appear to be a sensitive factor in predicting the neoplastic potential of dysplastic tissues. (hindawi.com)
  • Epidermal growth factor receptor (EGFR), a tyrosine kinase receptor of the ErbB family, and the biological receptor of EGF and TGF- α are expressed or highly expressed in a variety of solid tumors, including oral cancers. (hindawi.com)
  • Epidermal growth factor receptor (EGFR, ErbB1, and HER1)-the first receptor tyrosine kinase, was discovered by Carpenter and coworkers at Vanderbilt University, USA, in 1978 [ 3 ]. (hindawi.com)
  • Therefore, we studied the effects of two AGE precursors, glyoxal (GO) and methylglyoxal (MGO), on the epidermal growth factor receptor (EGFR) signaling pathway in cultured cells. (diabetesjournals.org)
  • Our investigations were mainly focused on the epidermal growth factor receptor (EGFR) because high glucose concentrations lead to abnormal epidermal growth factor (EGF) signaling ( 14 ) and diabetes reduces EGFR autophosphorylation ( 15 - 17 ). (diabetesjournals.org)
  • The EGFR belongs to a large family of tyrosine kinase receptors and is involved in the regulation of multiple cellular processes, such as cell growth, motility, differentiation, survival, and death. (diabetesjournals.org)
  • The EGFR is a 170-kDa transmembrane receptor tyrosine kinase that is shared by several growth factors, including EGF, heparin-binding EGF, transforming growth factor-α, amphiregulin, neuroregulin, β-cellulin, and epiregulin ( 18 ). (diabetesjournals.org)
  • The epidermal growth factor receptor (EGFR) and its ligands have been long recognized as centrally involved in the growth and repair process of epithelia, as well as in carcinogenesis. (springer.com)
  • Chow NH, Tzai TS, Lin SN, Chan SH, Tang MJ (1993) Reappraisal of the biological role of epidermal growth factor recepter (EGFR) in transitional cell carcinoma. (springer.com)
  • The epidermal growth factor receptor (EGFR, also known as ErbB1) is the first of four members of the ErbB lineage of receptor tyrosine kinases. (springer.com)
  • EGFR gene amplification in breast cancer: correlation with epidermal growth factor receptor mRNA and protein expression and HER-2 status and absence of EGFR-activating mutations. (springer.com)
  • Many epithelial cancers rely on enhanced expression of the epidermal growth factor receptor (EGFR) to drive proliferation and survival pathways. (mdpi.com)
  • This study was prospectively designed to evaluate a phase II study of gefitinib for non-small-cell lung cancer (NSCLC) patients with epidermal growth factor receptor (EGFR) mutations. (nature.com)
  • A Cox proportional hazards model indicated that negative EGFR mutation was a secondary prognostic factor (hazards ratio: 2.259, P =0.036). (nature.com)
  • Detection of the extracellular domain of the epidermal growth factor receptor (EGFR ECD) in serum has been suggested as a potential biomarker for monitoring this effect in vivo. (cdc.gov)
  • The feasibility of using an enzyme linked immunosorbent assay (ELISA) to detect the extracellular domain (ECD) of the epidermal growth factor receptor (EGFr) in the serum of asbestosis patients was examined and the role of EGFr over expression in asbestos mediated carcinogenesis was evaluated. (cdc.gov)
  • Sensitizing mutations in epidermal growth factor receptor ( EGFR ) gene ( EGFR m + ), such as exon 19 deletions and exon 21 L858R point mutations, are the most important drivers in NSCLC patients. (dovepress.com)
  • The epidermal growth factor receptor (EGFR), a long-standingdrug development target, is also a desirable target for imaging. (unt.edu)
  • Recently it has been reported that mutations in the tyrosine kinase domain of the epidermal growth factor receptor ( EGFR ) gene occur in a subset of patients with lung cancer showing a dramatic response to EGFR tyrosine kinase inhibitors. (aacrjournals.org)
  • Panitumumab is an investigational fully human monoclonal antibody that targets the epidermal growth factor receptor (EGFr), a protein that plays an important role in cancer cell signaling. (thefreedictionary.com)
  • ERBITUX is an IgG1 monoclonal antibody (IgG1 MAb) designed to inhibit the function of a molecular structure expressed on the surface of normal and tumor cells called the epidermal growth factor receptor (EGFR, HER1, c-ErbB-1). (thefreedictionary.com)
  • IMC-11F8, currently in Phase I testing in Europe, is a fully human, high-affinity antibody that blocks ligand-dependent activation of the epidermal growth factor receptor (EGFR). (thefreedictionary.com)
  • The targets of these studies included epidermal growth factor receptor (EGFR), vascular endothelial growth factor recptor-2 (VEGFR-2), insulin-like growth factor-1 receptor (IGF-1R), platelet-derived growth factor receptor alpha (PDGFRa), FMS-like tyrosine kinase 3 receptor (FLT3), and macrophage stimulating 1 receptor (RON), among others. (thefreedictionary.com)
  • Two types of epidermal growth factor receptor ( EGFR ) mutations in exon 19 and exon 21 (ex19del and L858R) are prevalent in lung cancer patients and sensitive to targeted EGFR inhibition. (dovepress.com)
  • A mechanism by which β2-adrenergic receptors (β2ARs) stimulate signaling is transactivation of the epidermal growth factor receptor (EGFR), a cardioprotective signaling pathway that requires the formation of a β2AR-EGFR complex and the activation of Src. (ahajournals.org)
  • Epidermal growth factor receptor (EGFR) plays a central role in the progression of several human malignancies. (urotoday.com)
  • RATIONALE: Studying samples of tissue and blood in the laboratory from patients with cancer receiving epidermal growth factor receptor ( EGFR ) inhibitors may help doctors understand the effects of EGFR inhibitor therapy in the skin. (clinicaltrials.gov)
  • When NEU3 was stably transfected with or without EGFR, it was associated with significant increases in clonogenic growth, clonogenicity on soft agar and in vivo tumor growth in nude mice either with or without the receptor overexpression in the presence of EGF, compared with the levels in their vector controls. (sigmaaldrich.com)
  • The NEU3-mediated activation was largely abrogated by the EGFR inhibitor AG1478 or PD153035, but significant clonogenic growth still remained. (sigmaaldrich.com)
  • The present study revealed that PQQ induces the activation (tyrosine autophosphorylation) of epidermal growth factor receptor (EGFR) and its downstream signaling in a ligand-independent manner, leading to increased cellular proliferation in an epithelial cell line A431. (sigmaaldrich.com)
  • The interaction of the activated epidermal growth factor (EGF) receptor (EGFR) with the Src homology 2 (SH2) domain of Grb2 (growth-factor-receptor-bound protein 2) initiates signalling through Ras and mitogen-activated protein kinase. (portlandpress.com)
  • This study seeks to determine whether the addition of ABT-414 to concomitant radiotherapy and temozolomide (TMZ) followed by combination of ABT-414 with adjuvant TMZ prolongs overall survival (OS) among participants with newly diagnosed glioblastoma (GBM) with epidermal growth factor receptor (EGFR) amplification. (clinicaltrials.gov)
  • The effect of changing the ganglioside composition of Chinese hamster ovary K1 cells on the function of the epidermal growth factor receptor (EGFr) was examined by studying the signalling pathway generated after the binding of epidermal growth factor (EGF) both in cells depleted of glycosphingolipids by inhibiting glucosylceramide synthase activity and in cell lines expressing different gangliosides as the result of stable transfection of appropriate ganglioside glycosyltransferases. (biochemj.org)
  • We hypothesized that the GLP-1R could activate PI 3-kinase and promote β-cell proliferation through transactivation of the epidermal growth factor (EGF) receptor (EGFR), an event possibly linked to GPCRs via activation of c-Src and the production of putative endogenous EGF-like ligands. (diabetesjournals.org)
  • Epidermal growth factor receptor (EGFR) is an intriguing target in high-grade gliomas because it is frequently overexpressed due to amplification of the EGFR gene. (aacrjournals.org)
  • Retrospective correlative analyses generated a plethora of putative predictive factors of activity of EGFR tyrosine kinase inhibitors. (aacrjournals.org)
  • Epidermal growth factor receptor (EGFR) amplification and overexpression, present in ∼50% of glioblastomas, are associated with a poor prognosis, especially when occurring in younger patients ( 5 , 6 ). (aacrjournals.org)
  • Because an epidermal growth factor receptor (EGFR) cascade induces MUC5AC mucin in airways, and because EGFR is up-regulated in asthmatic airways, we examined the effect of eosinophils on MUC5AC mucin production in NCI-H292 cells (a human airway epithelial cell line that produces mucins). (jimmunol.org)
  • Mucin production in airways is induced by an epidermal growth factor receptor (EGFR) 3 cascade ( 13 ). (jimmunol.org)
  • We found that phenobarbital activated CAR by inhibiting epidermal growth factor receptor (EGFR) signaling. (sciencemag.org)
  • Specific activating mutations within the epidermal growth factor receptor ( EGFR ) identify a subset of non-small cell lung cancers with dramatic sensitivity to the specific tyrosine kinase inhibitors (TKI), gefitinib and erlotinib. (aacrjournals.org)
  • Somatic mutations in epidermal growth factor receptor ( EGFR ) seem to define a specific subset of non-small cell lung cancers (NSCLC), ∼10% of cases, which are most commonly adenocarcinomas and bronchoalveolar carcinomas arising in nonsmokers, with an increased prevalence in women and individuals of Asian ethnicity ( 1 - 4 ). (aacrjournals.org)
  • Most clinical studies have shown that NSCLC with rapid and dramatic responses to these tyrosine kinase inhibitors (TKI) harbor EGFR mutations, although long-term survival in TKI-treated patients may be affected by additional factors, including EGFR amplification, expression levels, and possibly other molecular markers ( 1 - 3 , 7 , 10 - 24 ). (aacrjournals.org)
  • This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. (jove.com)
  • This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative fluorescence microscopy and environmental scanning electron microscopy (ESEM) of whole cells in hydrated state. (jove.com)
  • To understand the dynamic operation of signaling cascades, we have developed a method enabling the simultaneous quantification of tyrosine phosphorylation of specific residues on dozens of key proteins in a time-resolved manner, downstream of epidermal growth factor receptor (EGFR) activation. (mcponline.org)
  • Cell signaling downstream of receptor tyrosine kinases, such as epidermal growth factor receptor (EGFR), 1 comprises an interconnected network of pathways associated with various regulatory processes. (mcponline.org)
  • AbstractBackground.Postprogression repeat biopsies are critical in caring for patients with lung cancer with epidermal growth factor receptor (EGFR) mutations. (medworm.com)
  • A significant functional role for epidermal growth factor receptor (EGFR) in the suprachiasmatic nucleus is suggested by recent findings that epidermal growth factor receptor and its ligand transforming growth factor-α are highly expressed in the suprachiasmatic nucleus. (ovid.com)
  • Since epidermal growth factor receptor (EGFR), like S100A7, is often expressed in estrogen receptor-alpha-negative (ERalpha-) breast cancer, we set out to investigate the role of Jab1 in mediating EGFR signaling, another facet of the ERalpha- phenotype. (nih.gov)
  • Jab1 is a target of EGFR signaling in ERalpha- cell lines and breast tumors and therefore may be a common central factor and potential therapeutic target for important cell signaling pathways in ERalpha- breast cancer. (nih.gov)
  • It has also been reported that epidermal growth factor receptor (EGFR) is necessary for HCMV-mediated signaling and entry (X. Wang, S. M. Huong, M. L. Chiu, N. Raab-Traub, and E. E. Huang, Nature 424:456-461, 2003). (asm.org)
  • Integrins are known to signal synergistically with growth factor receptors, and this coordination was recently reported for EGFR and β3 integrins in the context of HCMV entry (X. Wang, D. Y. Huang, S. M. Huong, and E. S. Huang, Nat. (asm.org)
  • Signaling through the epidermal growth factor receptor (EGFR) has been shown to result in NF-κB activation. (aacrjournals.org)
  • In 2009, molecular target therapy such as epidermal growth factor receptor (EGFR) inhibitors was recognized as a potential treatment for certain types of lung cancer [ 1 , 2 ]. (medsci.org)
  • The epidermal growth factor receptor (EGFR)/HER‑2 specific small molecule inhibitor, lapatinib (LAP), was used to select the drug resistant phenotype. (spandidos-publications.com)
  • Phosphorylation of tyrosine residues on the epidermal growth factor (EGF) receptor (EGFr) is an important early event in signal transduction, leading to cell replication for major human carcinomas. (aspetjournals.org)
  • Inhibition of EGFr phosphotyrosine in an ex vivo assay format effectively estimates the potency and degree of inhibition of EGFr-dependent human LICR-LON-HN5 head and neck carcinoma tumor growth. (aspetjournals.org)
  • Provided are anti-epidermal growth factor receptor (EGFR) antibodies, aglycosylated CDR-H2 anti-EGFR antibodies, and antigen binding fragments thereof. (patents.com)
  • To determine the expression of nucleostemin (NS), epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) mRNA in human esophageal squamous cell carcinoma (ESCC) tissues and their association in a human ESCC cell line. (ebscohost.com)
  • Background: EGFR is involved in the epidermal growth factors pathway that regulates cellular processes and is associated with the development of many types of cancer including colorectal cancer. (ebscohost.com)
  • To evaluate the clinical significance of mRNA expression of cytokeratin 19 (CK19), epidermal growth factor receptor (EGFR) and lung-specific X protein (LUNX), a total of 42 patients who were diagnosed with non-small cell lung cancer (NSCLC) by pathology were studied retrospectively. (ebscohost.com)
  • This month's installment of Clinical Trials Referral Resource is devoted to studies regarding epidermal growth factor receptor inhibitors. (cancernetwork.com)
  • Here we show that stimulation of epidermal growth factor receptors (EGF-R) by its ligands, EGF and transforming growth factor α (TGFα), causes MUC5AC expression in airway epithelial cells both in in vitro and in vivo . (pnas.org)
  • With the understanding of the mechanism of malignant transformation has come the knowledge that oncogene products are frequently growth factors, growth factor receptors, or elements of growth factor signal-transduction pathways. (cancernetwork.com)
  • Recent findings suggest that the cellular response to DNA damage ismarkedly impaired by deprivation of essential growth factors or by blockage ofgrowth-factor receptors, which suggests that these pathways contribute to theineffectiveness of chemotherapy and radiation. (cancernetwork.com)
  • The ErbB family of growth-factor receptors is well characterizedand has generated significant interest as a target for cancer therapeutics. (cancernetwork.com)
  • Because family members can be activated by multiple ligands andligand-receptor expression determines homo/heterodimerization between receptorsas well as rate of receptor internalization and degradation, the efficiency anddiversity of signal transduction through these receptor complexes is remarkable.Activation of this family of growth-factor receptors influences cellproliferation, survival, motility, adhesion, invasion, and angiogenesis. (cancernetwork.com)
  • The proliferation and differentiation of cancer in the body are usually controlled by growth factors and their receptors on cancer cell surface. (hindawi.com)
  • The human epidermal growth factor receptor (HER) family of receptors plays a central role in the pathogenesis of several human cancers. (hindawi.com)
  • It was hypothesized that AGEs alter cell signaling by interfering with growth factor receptors. (diabetesjournals.org)
  • Because altered response to growth factor may be implicated in the pathogenesis of diabetic ulcers ( 12 ) and vascular diseases associated with diabetes ( 13 ), we hypothesized that GO and MGO may modify growth factor receptors and alter the subsequent signaling. (diabetesjournals.org)
  • Abnormal polarization of EGF receptors and autocrine stimulation of cyst epithelial growth in human ADPKD. (springer.com)
  • EGFs are secreted by cells and released into the external environment where they may bind to epidermal growth factor receptors or EGFRs. (wikibooks.org)
  • Epidermal Growth Factors and Receptors were found by Nobel Prize winner Stanley Cohen. (wikibooks.org)
  • Receptors for growth hormone and prolactin. (dissertations.se)
  • CIN85 associates with multiple effectors controlling intracellular trafficking of epidermal growth factor receptors. (diva-portal.org)
  • CIN85 is a multidomain adaptor protein involved in Cbl-mediated down-regulation of epidermal growth factor (EGF) receptors. (diva-portal.org)
  • Human epidermal growth factor receptor‑2 (HER‑2) expression in the presence or absence of hormone receptors dictates HER‑2‑targeted therapy with or without endocrine therapy. (spandidos-publications.com)
  • For the majority of human carcinomas, growth factor receptors play an important role in tumorigenesis and progression to terminal disease states. (aspetjournals.org)
  • Increased expression of the epidermal growth factor (EGF) receptors, HER1 and HER2 are related to poor prognosis in most cancers studied. (ebscohost.com)
  • Canguilhem B, Pradines A, Baudouin C, Boby C, Lajoie-Mazenc I, Charveron M, Favre G (2005) RhoB protects human keratinocytes from UVB-induced apoptosis through epidermal growth factor receptor signaling. (springer.com)
  • Spatial regulation of epidermal growth factor receptor signaling by endocytosis. (springer.com)
  • In doing so, proteins attach to the newly phosphorylated tyrosines thus activating a signaling cascade that ends with DNA synthesis and cell growth. (wikibooks.org)
  • However, the exact mechanism by which the GLP-1 receptor (GLP-1R), a member of the G protein-coupled receptor (GPCR) superfamily, activates the PI 3-kinase signaling pathway to promote β-cell growth remains unknown. (diabetesjournals.org)
  • Proposed model depicting the epidermal growth factor receptor ‐extracellular‐regulated kinase‐TRIM32 signaling axis in the regulation of neuronal differentiation. (medworm.com)
  • A, Under myelin‐associated inhibition, protein kinase C‐, Src‐Pyk2‐induced epidermal growth factor receptor‐extracellular‐regulated kinase signaling pathway was activated, resulting in TRIM32 inhibition and neuronal differentiation of neural stem cells inhibition. (medworm.com)
  • Ang II induces phosphorylation of the epidermal growth factor (EGF) receptor (EGF-R), which serves as a scaffold for various signaling molecules. (ahajournals.org)
  • These findings emphasize the importance of ROS in specific Ang II-stimulated growth-related signaling pathways and suggest that redox-sensitive EGF-R transactivation may be a potential target for antioxidant therapy in vascular disease. (ahajournals.org)
  • Integrating mechanistic studies with analyses of tumor tissue from patients treated in clinical trials, Mischel and colleagues discovered signaling, transcriptional, and metabolic co-dependencies that are downstream consequences of oncogene amplification, including alterations in glucose and lipid metabolism that drive tumor growth, progression and drug resistance. (wikipedia.org)
  • Holm-Bentzen M, Lose G, Sorensen K, Jorgensen L, Nexo E (1987) Chronic cystitis: excretion of epidermal growth factor (EGF)/urogastrone (URO). (springer.com)
  • This growth factor, obtained from retrovirus-transformed fibroblasts, is structurally and functionally related to mouse epidermal growth factor and human urogastrone. (sciencemag.org)
  • M.S. Urdea, J.P. Merryweather, G.T. Mullenbach, D. Coit, U. Heberlein, P. Valenzuela, and P.J. Barr, Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast, Proc. (springer.com)
  • Distinctive actions of epidermal growth factor-urogastrone in isolated smooth muscle preparations from guinea pig stomach: differential inhibition by indomethacin. (aspetjournals.org)
  • Epidermal growth factor-urogastrone (murine EGF-URO) caused concentration-dependent contractile responses in preparations of longitudinal and circular smooth muscle derived from guinea pig stomach. (aspetjournals.org)
  • The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. (wikipedia.org)
  • Epidermal growth factor receptor inhibition strategies in pancreatic cancer: past, present and the future. (thefreedictionary.com)
  • A small molecule called PD 153035 inhibited the epidermal growth factor (EGF) receptor tyrosine kinase with a 5-pM inhibition constant. (sciencemag.org)
  • Go6976 and PP2 were used to inhibit PKC and Src‐Pyk2, respectively, which resulted in epidermal growth factor receptor phosphorylation inhibition indirectly. (medworm.com)
  • Epidermal growth factor receptor‐extracellular‐regulated kinase blockade can promote TRIM32 expression and antagonize myelin inhibition on neuronal differentiation of neural stem cells. (medworm.com)
  • Substantial growth inhibition of human tumor xenografts was achieved with p.o. doses of the compound (ED 50 = 10 mg/kg q.d. for 20 days). (aspetjournals.org)
  • Clinical and biological features associated with epidermal growth factor receptor gene mutations in lung cancers. (springer.com)
  • The present study will investigate the feasibility and clinical value of using circulating tumor DNA as selection for anti-epidermal growth factor receptor treatment for metastatic colorectal cancer. (centerwatch.com)
  • Abstract -Angiotensin II (Ang II) is a vasoactive hormone with critical roles in vascular smooth muscle cell growth, an important feature of hypertension and atherosclerosis. (ahajournals.org)
  • HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. (wikipedia.org)
  • For instance, MGO inhibits mitochondrial respiration, membrane ATPases and glyceraldehyde-3-phosphate dehydrogenases ( 10 ), and DNA and protein synthesis, thereby inducing growth arrest and cell death ( 11 ). (diabetesjournals.org)
  • The epidermal growth factor receptor (erbB1)is a dimeric membrane bound protein responsible for an array of downstream growth factor activation after ligand binding. (oncolink.org)
  • A gene on chromosome 7p12 that encodes epidermal growth factor, a transmembrane glycoprotein of the protein kinase superfamily, which is a receptor for members of the epidermal growth factor family. (thefreedictionary.com)
  • We have previously shown that epidermal growth factor (EGF) augments cAMP accumulation in the heart and stimulates cardiac adenylyl cyclase via a G protein mediated mechanism (Nair et al. (amrita.edu)
  • SDS-PAGE of Mouse Epidermal Growth Factor Recombinant Protein Bioactivity of Mouse Epidermal Growth Factor Recombinant Protein. (antibodies-online.com)
  • Application Note: Epidermal Growth Factor Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-Epidermal Growth Factor in immunological assays. (antibodies-online.com)
  • One novel protein, the milk fat globule protein epidermal growth factor 8 (MFG-E8), increases 2.3-fold in abundance in old aorta. (ahajournals.org)
  • 7-14 VSMC invasion is also facilitated by an intimal-medial concentration gradient of monocyte chemoattractant protein (MCP)-1, and platelet-derived growth factor (PDGF)-BB. (ahajournals.org)
  • R.C. Baxter, Z. Zaltsman, and J.R. Turtle, Immunoreactive somatomedin-C/insulin-like growth factor 1 and its binding protein in human milk, J. Clin. (springer.com)
  • We previously provided evidence that glucagon-like peptide 1 (GLP-1) induces pancreatic β-cell growth nonadditively with glucose in a phosphatidylinositol (PI) 3-kinase- and protein kinase C ζ-dependent manner. (diabetesjournals.org)
  • Using cluster analysis, we observed coordinate upregulation of EGF, IL-6, macrophage inflammatory protein-1 beta and vascular endothelial growth factor. (diva-portal.org)
  • Mechanistically, Egfl7 prevented nuclear factor-κB nuclear localization and augmented inhibitor of nuclear factor-κB protein levels, suggesting that it inhibits nuclear factor-κB activation, a key step in the inflammatory activation of endothelial cells. (ahajournals.org)
  • Epidermal growth factor-like domain 7 (Egfl7) is a novel protein that has been found to be expressed exclusively by endothelial and early endothelial progenitor cells. (ahajournals.org)
  • Biotherapeutic approaches, employing probiotic and epidermal growth factor (EGF) could help in barrier protein protection and aid in CDAD management. (scirp.org)
  • 2. Addition of epidermal growth factor at a concentration of 5 or 10 ng/ml increased the synthesis of glycoprotein from 84 ± 16.2 d.p.m./μg of protein to 132 ± 19.5 and 156 ±20.2 d.p.m./μg of protein, respectively. (portlandpress.com)
  • The results indicate that several transcription factors such as Elk1 and cAMP-responsive element binding protein/activating transcription factor family, known to be 'input points' to the core clock network, are playing a role. (ovid.com)
  • MDA-MB-231 and MDA-MB-468 ERalpha-/EGFR+ cell lines were assessed for localization of Jab1 and levels of downstream genes by immunofluorescence and nuclear protein extract assay following treatment with epidermal growth factor (EGF) and extracellular signal-regulated kinase (ERK) pathway inhibitor. (nih.gov)
  • J.-F. Beaulieu, and R. Calvert, The effect of epidermal growth factor (EGF) on the differentiation of the rough endoplasmic reticulum in fetal mouse small intestine in organ culture, J. Histochem. (springer.com)
  • The name epidermal growth factor was first used in the initial reports of these studies. (docme.ru)
  • Growth factors could be involved in goblet-cell production, because hypersecretory diseases are associated with abnormal epithelial-cell growth and proliferation. (pnas.org)
  • They regulate cell growth, survival, and differentiation via multiple signal transduction pathways and participate in cellular proliferation and differentiation. (hindawi.com)
  • An epidermal growth factor or EGF is a type of growth factor that controls the proliferation, differentiation and survival of a cell. (wikibooks.org)
  • Transforming growth factor-α (TGF-α)-promotes neural cell proliferation. (wikibooks.org)
  • Pyrroloquinoline quinone stimulates epithelial cell proliferation by activating epidermal growth factor receptor through redox cycling. (sigmaaldrich.com)
  • Finally, coculturing the prostatic cancer cell line LNCaP that lacks GLP-1 responsiveness with INS cells increased LNCaP cell proliferation in the presence of GLP-1, thus revealing that INS cells secrete a growth factor in response to GLP-1. (diabetesjournals.org)
  • In a variety of studies, growth factors have been identified to be involved in maintaining epithelial renewal or epithelial cell proliferation. (molvis.org)
  • Although eIF5A is intimately involved in eukaryotic cell proliferation, the true physiologic function of this essential factor has yet to be elucidated, and the potential role of eIF5A needs further investigation. (molvis.org)
  • EGF-R gene expression was stimulated further by tumor necrosis factor α (TNFα). (pnas.org)
  • Moreover, EGF-R is known to be up-regulated by the proinflammatory cytokine tumor necrosis factor α (TNFα) ( 14 - 16 ), which is increased in lungs in hypersecretory diseases ( 17 ). (pnas.org)
  • Herr HW, Badalament RA, Amato DA, Laudone VP, Fair W, Whitmore WF (1989) Superficial bladder cancer treated with bacillus Calmette-Guérin: a multivariate analysis of factors affecting tumor progression. (springer.com)
  • Insulin-Like Growth Factor I Protects Oligodendrocytes from Tumor Necrosis Factor-α-Induced Injury", Endocrinology, 140(7):3063-3072(1999). (freepatentsonline.com)
  • We analyzed pretreatment MR imaging scans from 147 consecutive patients with newly diagnosed glioblastoma and correlated MR imaging features with tumor epidermal growth factor receptor amplification status. (ajnr.org)
  • The prognostic significance of proliferating cell nuclear antigen, epidermal growth factor receptor , and mdr gene expression in colorectal cancer. (thefreedictionary.com)
  • Neuregulin-2 (NRG2-induces growth and differentiation of epithelial, neuronal, glial, and other cell types. (wikibooks.org)
  • Finally, GLP-1 induces several immediate early response genes and proto-oncogenes in INS cells that are implicated in cell growth/apoptosis control, such as c- fos , c- jun , junD, and nur77 ( 15 , 16 ). (diabetesjournals.org)
  • Recent studies indicate that epidermal growth factor receptor activation induces behavioral and physiological effects, strengthening the notion that epidermal growth factor receptor can modulate suprachiasmatic nucleus neural function and behavior. (ovid.com)
  • Here, we showed for the first time that epidermal growth factor (EGF) induces S100A7 expression in both MCF-7 and MDA-MB-468 cell lines. (harvard.edu)
  • Epidermal growth factor induces phosphorylation of extracellular signal-regulated kinase 2 via multiple pathways. (asm.org)
  • Flipped script for gefitinib: a reapproved tyrosine kinase inhibitor for first-line treatment of epidermal growth factor receptor mutation positive metastatic nonsmall cell lung cancer. (springer.com)
  • In renal cell carcinoma (RCC) models, maximal cytotoxicity of the proteasome inhibitor bortezomib is dependent on efficient blockade of constitutive nuclear factor κB (NF-κB) activity. (aacrjournals.org)
  • Expression and methylation status of tissue factor pathway inhibitor-2 gene in non-small-cell lung cancer. (ebscohost.com)
  • Tissue factor pathway inhibitor-2 (TFPI-2) is a Kunitz-type serine proteinase inhibitor that inhibits plasmin-dependent activation of several metalloproteinases. (ebscohost.com)
  • It promotes growth and differentiation, is essential in embryogenesis, and is also important in wound healing. (thefreedictionary.com)
  • A cell surface receptor involved in regulation of cell growth and differentiation. (harvard.edu)
  • Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. (prospecbio.com)
  • EGF is capable not only of promoting epithelial cell growth but also differentiation in the fetal rabbit lung. (ovid.com)
  • GLP-1 increases the expression level of the β-cell-specific transcription factor pancreatic and duodenal homeobox gene-1 (PDX-1) ( 9 ), which is implicated in the regulation of the expression of insulin, GLUT2, and glucokinase genes and in β-cell differentiation ( 10 - 12 ). (diabetesjournals.org)
  • Balagula Y, Garbe C, Myskowski PL, Hauschild A, Rapoport BL, Boers-Doets CB, Lacouture ME (2011) Clinical presentation and management of dermatological toxicities of epidermal growth factor receptor inhibitors. (springer.com)
  • The HER2-HER3 heterodimer is the most potent stimulator of downstream pathways, particularly the PI3K/Akt, a master regulator of cell growth and survival. (hindawi.com)
  • Heparin-binding epidermal growth factor-like growth factor (HB-EGF) and proteolytic processing by a disintegrin and metalloproteinases (ADAM): a regulator of several pathways. (springer.com)
  • Epidermal growth factor receptor-dependent mechanisms have been implicated in growth signal transduction pathways that contribute to cancer development, including dermal carcinogenesis. (cdc.gov)
  • The results indicate that the interactions between estrogen, progesterone and epidermal growth factor receptor pathways may be considered relevant targets for the treatment of hormone-dependent breast cancers," she said. (thefreedictionary.com)
  • Fuse H, Mizuno I, Sakamoto M, Kataya AT (1992) Epidermal growth factor in urine from patients with urothelial tumors. (springer.com)
  • Kristensen JK, Lose G, Lund F, Nexo E (1988) Epidermal growth factor in urine from patients with urinary bladder tumors. (springer.com)
  • The first observation was that of Dr. Rita Levi-Montalcini who noted that certain mouse tumors, when implanted into chick embryos, released a factor that stimulated the growth of specific embryonic neurons. (docme.ru)
  • Epidermal growth factors is a class of EGF proteins. (wikibooks.org)
  • Kaur, S. , Vaishnavi, C. , Ray, P. , Singh, M. and Kochhar, R. (2014) Preliminary Investigation on the Effect of Lactobacillus and Epidermal Growth Factor on Tight Junction Proteins in Experimental Clostridium difficile Infection. (scirp.org)
  • These effectors include phosphatidyl-inositol phosphatases SHIP-1 and synaptojanin 2B1, Arf GTPase-activating proteins ASAP1 and ARAP3, adaptor proteins Hip1R and STAP1, and a Rho exchange factor, p115Rho GEF. (diva-portal.org)
  • Epidermal growth factor pathway substrate 15, Eps15. (nih.gov)
  • EGF dysregulation is linked to the growth and progression of certain cancers. (thefreedictionary.com)
  • Simon N, FitzGerald D. Immunotoxin Therapies for the Treatment of Epidermal Growth Factor Receptor-Dependent Cancers. (mdpi.com)
  • The present invention relates to a method for preparing a fusion polypeptide comprising epidermal growth factor (EGF) and human serum albumin in a plant. (freepatentsonline.com)
  • Epidermal Growth Factor or (E.G.F) is a polypeptide developed to help speed wound recovery. (joannavargas.com)
  • A polypeptide (or molecular chain of amino acids) developed to speed wound recovery, EGF stimulates skin growth at the cellular level. (joannavargas.com)
  • The second biological observation was made during my study of the nerve growth factor detected in male mouse submaxillary glands. (docme.ru)
  • Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor receptor family having tyrosine kinase activity. (hindawi.com)
  • HER2 is expressed in many tissues and its major role in these tissues is to facilitate excessive/uncontrolled cell growth and tumorigenesis [ 7 - 9 ]. (hindawi.com)
  • Carperter G, Cohen S (1990) Epidermal growth factor. (springer.com)
  • Epigallocatechin-3-gallate inhibits the growth and increases the apoptosis of human thyroid carcinoma cells. (greenmedinfo.com)
  • when injected into newborn animals, it accelerates eyelid opening and tooth eruption, stimulates epidermal growth and keratinization, and, in larger doses, inhibits body growth and hair development and produces fatty livers. (thefreedictionary.com)
  • Amphiregulin (AR-promotes growth of the normal epithelial cells and it inhibits growth of aggressive carcinoma cell lines. (wikibooks.org)
  • Recent studies have shown that milk fat globule-epidermal growth factor-8 (MFGE8), which promotes phagocytosis and inhibits inflammation, is an endogenous protective factor in response to brain infarction, Alzheimer's disease, subarachnoid hemorrhage, and prion disease. (frontiersin.org)
  • Involved in cell growth regulation. (rcsb.org)
  • Cetuximab: an epidermal growth factor receptor monoclonal antibody for the treatment of colorectal cancer. (thefreedictionary.com)
  • Among the growth factors, a possible candidate is epidermal growth factor (EGF) and its receptor (EGF-R). EGF-R, a 170-kDa membrane glycoprotein, is expressed on the surface of various cells, and may be related to mucin production in stomach ( 7 ), urothelium ( 8 ), and other epithelia ( 9 ). (pnas.org)
  • High incidence of amplification of the epidermal growth factor receptor gene in human squamous carcinoma cell lines. (nii.ac.jp)
  • Epidermal growth factor receptor gene amplification and expression in disseminated pediatric low-grade gliomas. (virtualtrials.com)
  • Epidermal growth factor receptor amplification is a common molecular event in glioblastomas. (ajnr.org)
  • The purpose of this study was to examine the potential usefulness of morphologic and diffusion MR imaging signs in the prediction of epidermal growth factor receptor gene amplification status in patients with glioblastoma. (ajnr.org)
  • 12). Quantitative DWI analysis found that all ADC measurements correlated with epidermal growth factor receptor amplification, with the highest correlations found with ADC ROI ( P = .0003) and ADC mean ( P = .0007). (ajnr.org)
  • Our results suggest a role for diffusion MR imaging in the determination of epidermal growth factor receptor amplification status in glioblastoma. (ajnr.org)
  • In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. (scirp.org)
  • Lau JLT, Fowler JE, Ghosh L (1988) Epidermal growth factor in the normal and neoplastic kidney and bladder. (springer.com)
  • Stimulation of metastatic MTLn3 cells with epidermal growth factor (EGF) causes a rapid and transient increase in actin nucleation activity resulting from the appearance of free barbed ends at the extreme leading edge of extending lamellipods. (rupress.org)
  • In the present study, we sought to investigate the different effects of both pretreated and posttreated recombinant milk fat globule-epidermal growth factor-8 (rhMFGE8) for the surgical brain injury (SBI) rat model and potential involvement of its receptor integrin β3 for apoptosis and neuroinflammation after SBI. (frontiersin.org)
  • Coutre PL, Bock S, Jakse G, Petrides PE (1992) Immunoreactive low-molecular-weight epidermal factor in urine of patients with renal cell carcinoma. (springer.com)
  • Cell and molecular biology of epidermal growth factor receptor. (springer.com)
  • It has been suggested that the transcription factor nuclear factor κB (NF-κB) represents a principal molecular target of bortezomib ( 10 ). (aacrjournals.org)
  • We have used a defined, serum-free medium to determine which of these growth factors becomes dispensable for the growth of transformed Syrian and Chinese hamster fibroblast cells. (pnas.org)
  • Serum provides growth factors that regulate and limit the growth of normal cells in tissue culture. (pnas.org)
  • Animal cells that are malignantly transformed usually exhibit diminished serum requirements for growth in culture. (pnas.org)
  • Mutagenesis with ethyl methanesulfonate can also create requirements of the transformed cells for unknown factors in serum. (pnas.org)
  • Why Do Cancer Cells Become "Addicted" to Oncogenic Epidermal Growth Factor Receptor? (plos.org)
  • It is found at abnormally high levels on the surface of many types of cancer cells, so these cells may divide excessively in the presence of epidermal growth factor. (hindawi.com)
  • Blanpain C, Fuchs E (2009) Epidermal homeostasis: a balancing act of stem cells in the skin. (springer.com)
  • Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. (springer.com)
  • The recent advances in this area have, somewhat unexpectedly, also provided mechanisms that may lead to a more detailed understanding of important biomedical questions, such as the growth behavior of malignant cells. (docme.ru)
  • Since these were whole animal experiments, we were faced with the problem of whether the factor operated directly on epidermal cells or whether growth was induced indirectly, possibly by the increased production of a more "classical" hormone. (docme.ru)
  • Human Oligodendrocyte Precursor Cells in Vitro: Phenotypic Analysis and Differential Response to Growth Factors", GLIA, 44(2):153-165(2003). (freepatentsonline.com)
  • Follicle cells express high levels of DER RNA during their mitotic phase of growth but lose these transcripts as they cease division. (biologists.org)
  • Peckys, D. B., de Jonge, N. Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy. (jove.com)
  • In conclusion, we found that rhMFGE8 pretreatment effectively alleviated neurological deficits and decreased brain water content and apoptotic cells in the SBI model through the MFGE8/integrin-β3 pathway, and treatment time was an important factor in achieving curative effects. (frontiersin.org)
  • Background- Epidermal growth factor-like domain 7 (Egfl7) is a chemoattractant for endothelial cells, and its expression is restricted to endothelial cells. (ahajournals.org)
  • Milk fat globule epidermal growth factor-factor 8 (MFG-E8) was originally identified for phagocytosis of apoptotic cells. (jimmunol.org)
  • However, the inflammatory factors underlying the negative effects of inflammation on the formation of new brain cells are not fully identified. (frontiersin.org)
  • In this study we applied small interference RNA (siRNA) to knock down the expression of eukaryotic translation initiation factor 5A (eIF5A) in corneal epithelial cells. (molvis.org)
  • A global transcriptional profiling study is performed to investigate the gene expression response to epidermal growth factor receptor activation in the suprachiasmatic nucleus. (ovid.com)
  • Recombinant human epidermal growth factor, sold under the brand name Heberprot-P, is used to treat diabetic foot ulcers . (wikipedia.org)
  • Lakshmanan J, Salido EC, Lam R, Fisher DA (1992) Epidermal growth factor prohormone is secreted in human urine. (springer.com)
  • 1-4 In humans, this age-associated arterial remodeling is an independent risk factor for the epidemic of quintessential human cardiovascular diseases, ie, atherosclerosis, hypertension, and stroke. (ahajournals.org)
  • The present invention is relevant to human medicine, and, in particular, to the use of Epidermal Growth Factor (EGF) in a pharmaceutical composition, said composition is administered through infiltration at the periphery of nerve trunks and/or ganglia, for the morphofunctional restoration of peripheral nerves in painful, sensory-motor neuropathy, as well as ischemic neuritis. (freepatentsonline.com)
  • Expression of the Drosophila homologue (DER) of the human epidermal growth factor (EGF) receptor has been studied during development by RNA blot hybridizations and in situ hybridizations. (biologists.org)
  • L.C. Read, F.M. Upton, G.L. Francis, J.C. Wallace, G.W. Dahlenburg, and F.J. Ballard, Changes in the growth-promoting activity of human milk during lactation, Pediatr. (springer.com)
  • In human malignant mesothelioma biopsies, SV40 has been shown to bind and inactivate p53 and pRb, and to activate c-met, insulin growth factor (IGF)-I, and other oncogenes. (aacrjournals.org)
  • 1997) Epidermal Growth Factor Attenuates Clostridium difficile Toxin A- and B-Induced Damage of Human Colonic Mucosa. (scirp.org)
  • 1. The effects of epidermal growth factor on the synthesis and secretion by human gastric mucosa of radiolabelled mucus glycoprotein have been studied in organ culture. (portlandpress.com)
  • Brogden NK, Mehalick L, Fischer CL, Wertz PW, Brogden KA (2012) The emerging role of peptides and lipids as antimicrobial epidermal barriers and modulators of local inflammation. (springer.com)
  • It is specific for EPIDERMAL GROWTH FACTOR and EGF related peptides including TRANSFORMING GROWTH FACTOR ALPHA, amphiregulin, and heparin-binding EGF-like growth factor. (harvard.edu)
  • Epidermal growth factor and corneal wound healing. (nih.gov)
  • Barrientos S, Stojadinovic O, Golinko MS, Brem H, Tomic-Canic M (2008) Growth factors and cytokines in wound healing. (springer.com)
  • Heparin-binding EGF-like growth factor (HB-EGF)-plays a role in wound healing, cardiac hypertrophy, and heart development. (wikibooks.org)
  • This progress on spatially heterogenous growth factor release from MAP scaffolds has great potential to improve complex wound treatment and advance the field of growth factor releasing scaffolds. (rsc.org)
  • it acts by binding the high-affinity cell surface receptor, epidermal growth factor receptor. (thefreedictionary.com)
  • CBD can be used as a novel therapeutic option to inhibit growth and metastasis of highly aggressive breast cancer subtypes including TNBC. (greenmedinfo.com)
  • Busam KJ, Capodieci P, Motzer R, Kiehn T, Phelan D, Halpern AC (2001) Cutaneous side-effects in cancer patients treated with the antiepidermal growth factor receptor antibody C225. (springer.com)
  • A combined approach of inhibiting both the hormones and the epidermal growth factor receptor may be beneficial for some women in treating hormone-dependent breast cancer. (thefreedictionary.com)
  • Epidermal growth factor receptor immunohistochemical reactivity in patients with American Joint Committee on Cancer Stage IV colon adenocarcinoma: implications for a standardized scoring system. (thefreedictionary.com)
  • The factor(s) that determine gefitinib sensitivity has long been an enigma. (aacrjournals.org)
  • B, Cetuximab and gefitinib can directly block epidermal growth factor receptor. (medworm.com)