A species of gram-positive, coccoid bacteria commonly isolated from clinical specimens and the human intestinal tract. Most strains are nonhemolytic.
A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.
A species of gram-positive, coccoid bacteria whose organisms are normal flora of the intestinal tract. Unlike ENTEROCOCCUS FAECALIS, this species may produce an alpha-hemolytic reaction on blood agar and is unable to utilize pyruvic acid as an energy source.
Infections caused by bacteria that retain the crystal violet stain (positive) when treated by the gram-staining method.
Nonsusceptibility of bacteria to the action of VANCOMYCIN, an inhibitor of cell wall synthesis.
Substances that reduce the growth or reproduction of BACTERIA.
Antibacterial obtained from Streptomyces orientalis. It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear.
Enzymes that catalyze the joining of two molecules by the formation of a carbon-oxygen bond. EC 6.1.
Proteins found in any species of bacterium.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
Bacteria which retain the crystal violet stain when treated by Gram's method.
A complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.
Chemical substances, excreted by an organism into the environment, that elicit behavioral or physiological responses from other organisms of the same species. Perception of these chemical signals may be olfactory or by contact.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Inflammation of the ENDOCARDIUM caused by BACTERIA that entered the bloodstream. The strains of bacteria vary with predisposing factors, such as CONGENITAL HEART DEFECTS; HEART VALVE DISEASES; HEART VALVE PROSTHESIS IMPLANTATION; or intravenous drug use.
Glycopeptide antibiotic complex from Actinoplanes teichomyceticus active against gram-positive bacteria. It consists of five major components each with a different fatty acid moiety.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Derivatives of oxazolidin-2-one. They represent an important class of synthetic antibiotic agents.
Derivatives of acetamide that are used as solvents, as mild irritants, and in organic synthesis.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
The functional hereditary units of BACTERIA.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The type species of gram negative bacteria in the genus ALCALIGENES, found in soil. It is non-pathogenic, non-pigmented, and used for the production of amino acids.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Coccus-shaped bacteria that retain the crystal violet stain when treated by Gram's method.
Chemicals used mainly to disinfect root canals after pulpectomy and before obturation. The major ones are camphorated monochlorophenol, EDTA, formocresol, hydrogen peroxide, metacresylacetate, and sodium hypochlorite. Root canal irrigants include also rinsing solutions of distilled water, sodium chloride, etc.
A genus of gram-positive, coccoid bacteria whose organisms occur in pairs or chains. No endospores are produced. Many species exist as commensals or parasites on man or animals with some being highly pathogenic. A few species are saprophytes and occur in the natural environment.
A cyclic lipopeptide antibiotic that inhibits GRAM-POSITIVE BACTERIA.
The space in a tooth bounded by the dentin and containing the dental pulp. The portion of the cavity within the crown of the tooth is the pulp chamber; the portion within the root is the pulp canal or root canal.
Nonsusceptibility of a microbe to the action of ampicillin, a penicillin derivative that interferes with cell wall synthesis.
Pheromones that elicit sexual attraction or mating behavior usually in members of the opposite sex in the same species.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-.
Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.
Substances that prevent infectious agents or organisms from spreading or kill infectious agents in order to prevent the spread of infection.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
A cyclic polypeptide antibiotic complex from Streptomyces virginiae, S. loidensis, S. mitakaensis, S. pristina-spiralis, S. ostreogriseus, and others. It consists of 2 major components, VIRGINIAMYCIN FACTOR M1 and virginiamycin Factor S1. It is used to treat infections with gram-positive organisms and as a growth promoter in cattle, swine, and poultry.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
It is used as an oxidizing and bleaching agent and as a disinfectant. (From Grant & Hackh's Chemical Dictionary, 5th ed)
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
Nonsusceptibility of bacteria to the action of TETRACYCLINE which inhibits aminoacyl-tRNA binding to the 30S ribosomal subunit during protein synthesis.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Endodontic diseases of the DENTAL PULP inside the tooth, which is distinguished from PERIAPICAL DISEASES of the tissue surrounding the root.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Inflammation of the DENTAL PULP, usually due to bacterial infection in dental caries, tooth fracture, or other conditions causing exposure of the pulp to bacterial invasion. Chemical irritants, thermal factors, hyperemic changes, and other factors may also cause pulpitis.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.
A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.
Any infection which a patient contracts in a health-care institution.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
A white powder prepared from lime that has many medical and industrial uses. It is in many dental formulations, especially for root canal filling.
A disinfectant and topical anti-infective agent used also as mouthwash to prevent oral plaque.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Inflammation of the PERIAPICAL TISSUE. It includes general, unspecified, or acute nonsuppurative inflammation. Chronic nonsuppurative inflammation is PERIAPICAL GRANULOMA. Suppurative inflammation is PERIAPICAL ABSCESS.
A specific streptogramin group A antibiotic produced by Streptomyces graminofaciens and other bacteria.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Substances used on humans and other animals that destroy harmful microorganisms or inhibit their activity. They are distinguished from DISINFECTANTS, which are used on inanimate objects.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
A family of gram-positive non-sporing bacteria including many parasitic, pathogenic, and saprophytic forms.
A genus of gram-positive, coccoid bacteria mainly isolated from milk and milk products. These bacteria are also found in plants and nonsterile frozen and dry foods. Previously thought to be a member of the genus STREPTOCOCCUS (group N), it is now recognized as a separate genus.
The presence of viable bacteria circulating in the blood. Fever, chills, tachycardia, and tachypnea are common acute manifestations of bacteremia. The majority of cases are seen in already hospitalized patients, most of whom have underlying diseases or procedures which render their bloodstreams susceptible to invasion.
Measurable quantity of bacteria in an object, organism, or organism compartment.
A group of QUINOLONES with at least one fluorine atom and a piperazinyl group.
A carboxypeptidase that is specific for proteins that contain two ALANINE residues on their C-terminal. Enzymes in this class play an important role in bacterial CELL WALL biosynthesis.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.
A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
A group of antibiotics that contain 6-aminopenicillanic acid with a side chain attached to the 6-amino group. The penicillin nucleus is the chief structural requirement for biological activity. The side-chain structure determines many of the antibacterial and pharmacological characteristics. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1065)
A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Pathogenicity from this genus is rare.
Inflammatory responses of the epithelium of the URINARY TRACT to microbial invasions. They are often bacterial infections with associated BACTERIURIA and PYURIA.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
A method where a culturing surface inoculated with microbe is exposed to small disks containing known amounts of a chemical agent resulting in a zone of inhibition (usually in millimeters) of growth of the microbe corresponding to the susceptibility of the strain to the agent.
Materials placed inside a root canal for the purpose of obturating or sealing it. The materials may be gutta-percha, silver cones, paste mixtures, or other substances. (Dorland, 28th ed, p631 & Boucher's Clinical Dental Terminology, 4th ed, p187)
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
A TETRACYCLINE analog, having a 7-dimethylamino and lacking the 5 methyl and hydroxyl groups, which is effective against tetracycline-resistant STAPHYLOCOCCUS infections.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A group of broad-spectrum antibiotics first isolated from the Mediterranean fungus ACREMONIUM. They contain the beta-lactam moiety thia-azabicyclo-octenecarboxylic acid also called 7-aminocephalosporanic acid.
A complex of cyclic peptide antibiotics produced by the Tracy-I strain of Bacillus subtilis. The commercial preparation is a mixture of at least nine bacitracins with bacitracin A as the major constituent. It is used topically to treat open infections such as infected eczema and infected dermal ulcers. (From Goodman and Gilman, The Pharmacological Basis of Therapeutics, 8th ed, p1140)
Infections by bacteria, general or unspecified.
A non-pathogenic species of LACTOCOCCUS found in DAIRY PRODUCTS and responsible for the souring of MILK and the production of LACTIC ACID.
An autolytic enzyme bound to the surface of bacterial cell walls. It catalyzes the hydrolysis of the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell wall glycopeptides, particularly peptidoglycan. EC 3.5.1.28.
A pyridoxal-phosphate protein that catalyzes the conversion of L-tyrosine to tyramine and carbon dioxide. The bacterial enzyme also acts on 3-hydroxytyrosine and, more slowly, on 3-hydroxyphenylalanine. (From Enzyme Nomenclature, 1992) EC 4.1.1.25.
Enzyme which catalyzes the peptide cross-linking of nascent CELL WALL; PEPTIDOGLYCAN.
A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
The ability of bacteria to resist or to become tolerant to several structurally and functionally distinct drugs simultaneously. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Suppurative inflammation of the tissues of the internal structures of the eye frequently associated with an infection.
Bacterial proteins that share the property of binding irreversibly to PENICILLINS and other ANTIBACTERIAL AGENTS derived from LACTAMS. The penicillin-binding proteins are primarily enzymes involved in CELL WALL biosynthesis including MURAMOYLPENTAPEPTIDE CARBOXYPEPTIDASE; PEPTIDE SYNTHASES; TRANSPEPTIDASES; and HEXOSYLTRANSFERASES.
The genetic complement of a BACTERIA as represented in its DNA.

Effects of dispersed recreational activities on the microbiological quality of forest surface water. (1/2300)

The microbiological quality of forest surface waters in the Greenwater River watershed was examined to investigate the influence of heavy motorized camping in an area with no sanitary facilities. Indicator densities increased during weekend human-use periods when compared to weekdays. Increases in indicator densities were also noted downstream from heavily used camping areas when compared to upstream sites. Seasonal, weekly, and diurnal fluctuations in indicator densities were observed. This study suggests that potential health hazards exist in this watershed during periods of human use.  (+info)

Isolation and chemical characterization of a capsular polysaccharide antigen shared by clinical isolates of Enterococcus faecalis and vancomycin-resistant Enterococcus faecium. (2/2300)

Enterococci are a common cause of serious infections, especially in newborns, severely immunocompromised patients, and patients requiring intensive care. To characterize enterococcal surface antigens that are targets of opsonic antibodies, rabbits were immunized with various gentamicin-killed Enterococcus faecalis strains, and immune sera were tested in an opsonophagocytic assay against a selection of clinical isolates. Serum raised against one strain killed the homologous strain (12030) at a dilution of 1:5,120 and mediated opsonic killing of 33% of all strains tested. In addition, this serum killed two (28%) of seven vancomycin-resistant Enterococcus faecium strains. Adsorption of sera with the homologous strain eliminated killing activity. The adsorbing antigens were resistant to treatment with proteinase K and to boiling for 1 h, but were susceptible to treatment with sodium periodate, indicating that the antigen inducing opsonic activity is a polysaccharide. Antibodies in immune rabbit sera reacted with a capsule-like structure visualized by electron microscopy both on the homologous E. faecalis strain and on a vancomycin-resistant E. faecium strain. The capsular polysaccharides from E. faecalis 12030 and E. faecium 838970 were purified, and chemical and structural analyses indicated they were identical glycerol teichoic acid-like molecules with a carbohydrate backbone structure of 6-alpha-D-glucose-1-2 glycerol-3-PO4 with substitution on carbon 2 of the glucose with an alpha-2-1-D-glucose residue. The purified antigen adsorbed opsonic killing activity from immune rabbit sera and elicited high titers of antibodies (when used to immunize rabbits) that both mediated opsonic killing of bacteria and bound to a capsule-like structure visualized by electron microscopy. These results indicate that approximately one-third of a sample of 15 E. faecalis strains and 7 vancomycin-resistant E. faecium strains possess shared capsular polysaccharides that are targets of opsonophagocytic antibodies and therefore are potential vaccine candidates.  (+info)

A new hydrolase specific for taurine-conjugates of bile acids. (3/2300)

Through the investigation of the bile acid-deconjugation activities of human intestinal anaerobes, a new enzyme was discovered in Peptostreptococcus intermedius which hydrolyzed specifically the taurine-conjugates, but not the glycine-conjugates of bile acids. However, the enzymes in Streptococcus faecalis and Lactobacillus brevis hydrolyzed chiefly the glycine-conjugates.  (+info)

Two-step acquisition of resistance to the teicoplanin-gentamicin combination by VanB-type Enterococcus faecalis in vitro and in experimental endocarditis. (4/2300)

The activity of vancomycin and teicoplanin combined with gentamicin was investigated in vitro against strains of Enterococcus faecalis resistant to vancomycin and susceptible to teicoplanin (VanB type) and against mutants that had acquired resistance to teicoplanin by three different mechanisms. In vitro, gentamicin selected mutants with two- to sixfold increases in the level of resistance to this antibiotic at frequencies of 10(-6) to 10(-7). Teicoplanin selected teicoplanin-resistant mutants at similar frequencies. Both mutations were required to abolish the activity of the gentamicin-teicoplanin combination. As expected, simultaneous acquisition of the two types of mutations was not observed. In therapy with gentamicin or teicoplanin alone, each selected mutants in three of seven rabbits with aortic endocarditis due to VanB-type E. faecalis BM4275. The vancomycin-gentamicin combination selected mutants that were resistant to gentamicin and to the combination. In contrast, the teicoplanin-gentamicin regimen prevented the emergence of mutants resistant to one or both components of the combination. These results suggest that two mutations are also required to suppress the in vivo activity of the teicoplanin-gentamicin combination.  (+info)

Efficacy of ampicillin plus ceftriaxone in treatment of experimental endocarditis due to Enterococcus faecalis strains highly resistant to aminoglycosides. (5/2300)

The purpose of this work was to evaluate the in vitro possibilities of ampicillin-ceftriaxone combinations for 10 Enterococcus faecalis strains with high-level resistance to aminoglycosides (HLRAg) and to assess the efficacy of ampicillin plus ceftriaxone, both administered with humanlike pharmacokinetics, for the treatment of experimental endocarditis due to HLRAg E. faecalis. A reduction of 1 to 4 dilutions in MICs of ampicillin was obtained when ampicillin was combined with a fixed subinhibitory ceftriaxone concentration of 4 micrograms/ml. This potentiating effect was also observed by the double disk method with all 10 strains. Time-kill studies performed with 1 and 2 micrograms of ampicillin alone per ml or in combination with 5, 10, 20, 40, and 60 micrograms of ceftriaxone per ml showed a > or = 2 log10 reduction in CFU per milliliter with respect to ampicillin alone and to the initial inoculum for all 10 E. faecalis strains studied. This effect was obtained for seven strains with the combination of 2 micrograms of ampicillin per ml plus 10 micrograms of ceftriaxone per ml and for six strains with 5 micrograms of ceftriaxone per ml. Animals with catheter-induced endocarditis were infected intravenously with 10(8) CFU of E. faecalis V48 or 10(5) CFU of E. faecalis V45 and were treated for 3 days with humanlike pharmacokinetics of 2 g of ampicillin every 4 h, alone or combined with 2 g of ceftriaxone every 12 h. The levels in serum and the pharmacokinetic parameters of the humanlike pharmacokinetics of ampicillin or ceftriaxone in rabbits were similar to those found in humans treated with 2 g of ampicillin or ceftriaxone intravenously. Results of the therapy for experimental endocarditis caused by E. faecalis V48 or V45 showed that the residual bacterial titers in aortic valve vegetations were significantly lower in the animals treated with the combinations of ampicillin plus ceftriaxone than in those treated with ampicillin alone (P < 0.001). The combination of ampicillin and ceftriaxone showed in vitro and in vivo synergism against HLRAg E. faecalis.  (+info)

The osmoprotectant glycine betaine inhibits salt-induced cross-tolerance towards lethal treatment in Enterococcus faecalis. (6/2300)

The response of Enterococcus faecalis ATCC 19433 to salt stress has been characterized previously in complex media. In this report, it has been demonstrated that this bacterium actively accumulates the osmoprotectant glycine betaine (GB) from salt-enriched complex medium BHI. To further understand the specific effects of GB and other osmoprotective compounds in salt adaptation and salt-induced cross-tolerance to lethal challenges, a chemically defined medium lacking putative osmoprotectants was used. In this medium, bacterial growth was significantly reduced by increasing concentrations of NaCl. At 0.75 M NaCl, 90% inhibition of the growth rate was observed; GB and its structural analogues restored growth to the non-salt-stressed level. In contrast, proline, pipecolate and ectoine did not allow growth recovery of stressed cells. Kinetic studies showed that the uptake of betaines shows strong structural specificity and occurs through a salt-stress-inducible high-affinity porter [Km = 3.3 microM; Vmax = 130 nmol min(-1) (mg protein)(-1); the uptake activity increased 400-fold in the presence of 0.5 M NaCl]. Moreover, GB and its analogues were accumulated as non-metabolizable cytosolic osmolytes and reached intracellular levels ranging from 1-3 to 1.5 micromol (mg protein)(-1). In contrast to the beneficial effect of GB on the growth of salt-stressed cultures of E. faecalis, its accumulation inhibits the salt-induced cross-tolerance to a heterologous lethal challenge. Indeed, pretreatment of bacterial cells with 0.5 M NaCl induced resistance to 0.3% bile salts (survival of adapted cells increased by a factor of 6800). The presence of GB in the adaptation medium reduced the acquisition of bile salts resistance 680-fold. The synthesis of 11 of the 13 proteins induced during salt adaptation was significantly reduced in the presence of GB. These results raise questions about the actual beneficial effect of GB in natural environments where bacteria are often subjected to various stresses.  (+info)

Microbiological and inflammatory effects of murine recombinant interleukin-10 in two models of polymicrobial peritonitis in rats. (7/2300)

A protective effect of interleukin-10 (IL-10) against the development of lethal shock has been demonstrated in various animal models. In contrast, the immunosuppressant properties of this mediator have been minimally evaluated in low-mortality models of infections. The clinical, microbiological, and inflammatory effects of murine recombinant IL-10 (mrIL-10) therapy were evaluated in two models of peritonitis in rats, which differed in the degree of severity of peritoneal inflammation 3 days after inoculation of Escherichia coli and Bacteroides fragilis with or without Enterococcus faecalis. The severity of the disease remained unchanged compared to that in control animals. A dose-related decrease in the peritoneal phagocyte count was observed in the treated groups compared to the counts in control animals. The subsequent experiments were performed exclusively in the mixed gram-positive-gram negative model, which exhibits an intense and prolonged inflammatory response with similar criteria. The early effects of mrIL-10 (evaluated 6 h after inoculation), repeated injections of mrIL-10 (four doses injected from 0 to 9 h after bacterial challenge), and pretreatment (two doses injected 6 and 3 h before inoculation) were evaluated. The clinical and microbiological parameters remained unchanged in the treated animals. Decreases in the peritoneal phagocyte count and the peritoneal concentration of tumor necrosis factor were observed following repeated injections of mrIL-10. In summary, our data suggest that mrIL-10 does not worsen the manifestations of sepsis. However, these results need to be confirmed in clinical practice.  (+info)

Isolation of Enterococcus faecalis clinical isolates that efficiently adhere to human bladder carcinoma T24 cells and inhibition of adhesion by fibronectin and trypsin treatment. (8/2300)

The adherence of Enterococcus faecalis strains to human T24 cells was examined by scanning electron microscopy. Five highly adhesive strains were identified from 30 strains isolated from the urine of patients with urinary tract infections. No efficiently adhesive strains were found among the 30 strains isolated from the feces of healthy students. The five isolated strains also adhered efficiently to human bladder epithelial cells. Analysis of restriction endonuclease-digested plasmid DNAs and chromosome DNAs showed that the five strains were different strains isolated from different patients. The adhesiveness of these strains was inhibited by treatment with fibronectin or trypsin, implying that a specific protein (adhesin) on the bacterial cell surface mediates adherence to fibronectin on the host cell surfaces, and the adhesin differs from the reported adhesins.  (+info)

Title:Antibacterial Flavonoids Against Oral Bacteria of Enterococcus Faecalis ATCC 29212 from Sarang Semut (Myrmecodia pendans) and Its Inhibitor Activity Against Enzyme MurA. VOLUME: 16 ISSUE: 3. Author(s):Dikdik Kurnia*, Eti Apriyanti, Cut Soraya and Mieke H. Satari. Affiliation:Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Padjadjaran-Bandung, Sumedang, Department of Chemistry, Faculty of Mathematic and Natural Sciences, Universitas Padjadjaran-Bandung, Sumedang, Department of Concervative Dentistry, Faculty of Dentistry, Syiah Kuala Universty, Banda Aceh, Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran-Bandung, Sumedang. Keywords:Sarang Semut, Myrmecodia pendans, Enterococcus faecalis, MurA enzyme and flavonoids, oral bacteria, PAC.. Abstract:. Background: A significant number of antibiotics are known to inhibit peptidoglycan synthesis in the cross-linking stage, while the drug fosfomycin is the only one known to inhibit MurA. ...
Since the advent of the modern antibiotic era there has been an increasing number of bacteria that are resistant to antibiotics. The growing antibiotic resistance and the lack in development of new antibiotics poses a serious public health threat worldwide. One of the first steps to combat this problem is to understand the molecular details associated with antibiotic resistance. Enterococcus faecalis is one of the most common causes of healthcare associated infections (HAI). E. faecalis is an opportunistic pathogen that normally resides in the gastrointestinal (GI) tracts of humans and other animals. This bacterium possesses intrinsic antibiotic resistance and can also acquire resistance to other antibiotics through horizontal gene transfer (HGT). Some multi-drug resistant (MDR) E. faecalis strain have acquired resistance vancomycin, an antibiotic of last-resort, leaving very few treatment options. It has been shown that MDR E. faecalis have expanded genomes enriched with mobile genetic elements ...
|i|Enterococcus|/i|, a Gram-positive facultative anaerobic cocci belonging to the lactic acid bacteria of the phylum Firmicutes, is known to be able to resist a wide range of hostile conditions such as different pH levels, high concentration of NaCl (6.5%), and the extended temperatures between 5°C and 65°C. Despite being the third most common nosocomial pathogen, our understanding on its virulence factors is still poorly understood. The current study was aimed to determine the prevalence of different virulence genes in |i|Enterococcus faecalis|/i| and |i|Enterococcus faecium|/i|. For this purpose, 79 clinical isolates of Malaysian enterococci were evaluated for the presence of virulence genes. |i|pilB, fms8, efaAfm|/i|, and |i|sgrA|/i| genes are prevalent in all clinical isolates. In conclusion, the pathogenicity of |i|E. faecalis|/i| and |i|E. faecium|/i| could be associated with different virulence factors and these genes are widely distributed among the enterococcal species.
BioAssay record AID 67253 submitted by ChEMBL: In vitro antibacterial activity against vancomycin resistant Enterococcus faecalis (VanA, BM4166).
TY - JOUR. T1 - Characterization of the first clinical isolate of vancomycin-resistant Enterococcus faecalis, AH803, in Taiwan. AU - Lu, Jang J.. AU - Ben, Ren J.. AU - Perng, Cherng L.. AU - Chi, Wei Ming. AU - Chu, Mong Ling. AU - Lee, Wei H.. PY - 2000/2. Y1 - 2000/2. N2 - We previously isolated a vancomycin-resistant strain of Enterococcus faecalis, designated AH803, from the sputum of a patient with pneumonia and bacteremia in Taiwan. AH803 was resistant to vancomycin (minimal inhibitory concentration, MIC = 512 μg/mL) but susceptible to teicoplanin (MIC = 8 μg/mL), and harbored the vanA gene but not the vanB gene. In this study, we further characterized E. faecalis AH803 and the plasmid it was found to contain. DNA from AH803 was analyzed for the presence of vanA and vanB resistance genes by polymerase chain reaction. The vancomycin resistant phenotype was transferable from AH803 to E. faecalis JH2-2, at a frequency of 4.8 x 10-2 AH803 was also resistant to gentamicin and ...
Molecular characterization and multilaboratory evaluation of Enterococcus faecalis ATCC 51299 for quality control of screening tests for vancomycin and high-level aminoglycoside resistance in enterococci.
TY - JOUR. T1 - Enterococcus faecalis bearing aggregation substance is resistant to killing by human neutrophils despite phagocytosis and neutrophil activation. AU - Rakita, Robert M.. AU - Vanek, Natalie N.. AU - Jacques-Palaz, Karen. AU - Mee, Mee. AU - Mariscalco, M. Michele. AU - Dunny, Gary M.. AU - Snuggs, Mark. AU - Van Winkle, W. Barry. AU - Simon, Scott I.. PY - 1999/11. Y1 - 1999/11. N2 - Enterococcus faecalis aggregation substance (AS) mediates efficient bacterium-bacterium contact to facilitate plasmid exchange as part of a bacterial sex pheromone system. We have previously determined that AS promotes direct, opsonin-independent binding of E. faecalis to human neutrophils (PMNs) via complement receptor type 3 and other receptors on the PMN surface. We have now examined the functional consequences of this bacterium-host cell interaction. AS-bearing E. faecalis was phagocytosed and internalized by PMNs, as determined by deconvolution fluorescence microscopy. However, these bacteria ...
BioAssay record AID 300437 submitted by ChEMBL: Antibacterial activity against Enterococcus faecalis ATCC 29212 after 24 hrs by broth microdilution test.
Bacteria can survive antibiotic treatment both by acquiring antibiotic resistance genes and through mechanisms of tolerance that are based on phenotypic changes and the formation of metabolically inactive cells. Here, we report an Enterococcus faecalis strain (E. faecalis UM001B) that was isolated from a cystic fibrosis patient and had no increase in resistance but extremely high-level tolerance to ampicillin, vancomycin, and tetracycline. Specifically, the percentages of cells that survived 3.5-h antibiotic treatment (at 100 μg · ml−1) were 25.4% ± 4.3% and 51.9% ± 4.0% for ampicillin and tetracycline, respectively; vancomycin did not exhibit any significant killing. Consistent with the changes in antibiotic susceptibility, UM001B was found to have reduced penetration of ampicillin and vancomycin and accumulation of tetracycline compared to the reference strain ATCC 29212. Based on whole-genome sequencing, four amino acid substitutions were identified in one of the tetracycline efflux ...
COVO MORALES, E.; DIAZ CABALLERO, A. e SIMANCAS PALLARES, M.. Gene expression esp (enterococcus surface protein) of enterococcus faecalis in an in vitro model of extracted teeth. Av Odontoestomatol [online]. 2016, vol.32, n.4, pp.195-204. ISSN 2340-3152.. Objective: To determine the presence and expression of Enterococcus faecalis Esp gene in several strains from an in vitro model on extracted teeth. Methods: An in vitro system was designed to evaluate the biofilm formation through fluorescence microscopy and gene expression that could be associated to biofilm formation. The system consisted of a previously extracted human tooth that was cut and prepared to provide by means of its root canal, an adequate surface for biofilm formation on behalf of Enterococcus faecalis. The system disposed an anaerobe chamber that allowed the growth of bacteria in broth culture and avoided contamination with other microorganisms. This chamber consisted of a sterile micro centrifuge tube, which was cut and united ...
Enterococcus faecalis biofilm traits and distribution characteristics in China have not been clarified. This study aimed to determine the prevalence and characteristics of E. faecalis biofilm formation in a sample of clinical isolates and to explore the virulence factors associated with biofilm formation in those isolates. A total of 265 E. faecalis isolates were collected from patients in Shenzhen, China. Virulence genes were detected within the genomes of the microbes by polymerase chain reaction (PCR). The isolates were subjected to multilocus sequence typing (MLST) based on housekeeping genes. Biofilms were detected by crystal violet staining. The expression levels of the clinical E. faecalis isolates genes were determined by quantitative real-time polymerase chain reaction. The prevalence of biofilm formation among E. faecalis clinical isolates was 47.2%. MLST yielded 44 different sequence types (STs). The main STs were ST16 and ST179; the ST16 isolates were more likely to form strong or medium
Enterococcus faecalis ATCC ® 700802D-5™ Designation: Genomic DNA from Enterococcus faecalis Strain V583 TypeStrain=False Application:
Enterococcus faecalis - formerly classified as part of the group D Streptococcus system - is a Gram-positive, commensal bacterium inhabiting the gastrointestinal tracts of humans and other mammals. Like other species in the genus Enterococcus, E. faecalis can cause life-threatening infections in humans, especially in the nosocomial (hospital) environment, where the naturally high levels of antibiotic resistance found in E. faecalis contribute to its pathogenicity. E. faecalis has been frequently found in root canal-treated teeth in prevalence values ranging from 30% to 90% of the cases. Root canal-treated teeth are about nine times more likely to harbor E. faecalis than cases of primary infections. E. faecalis is a nonmotile microbe; it ferments glucose without gas production, and does not produce a catalase reaction with hydrogen peroxide. It can produce a pseudocatalase reaction if grown on blood agar. The reaction is usually weak. It produces a reduction of litmus milk, but does not liquefy ...
Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalisisolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen. ...
Fingerprint Dive into the research topics of Antimicrobial resistance from Enterococci in a pediatric hospital. Plasmids in enterococcus faecalis isolates with high-level gentamicin and streptomycin resistance. Together they form a unique fingerprint. ...
Community-acquired urinary tract infection (CA-UTI) could be caused by endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired Enterococcus faecalis isolated from the urine of symptomatic patients and their fecal samples. Out of the studied patients, 63 pairs of E. faecalis isolates were obtained simultaneously from their urine and feces samples. All the strains were sensitive to vancomycin, linezolid, nitrofurantoin, and daptomycin (MIC value: ≤ 4 µg/ml), while resistance to tetracycline (urine: 88.9%; stool: 76.2%) and minocycline (urine: 87.3%, stool: 71.4%) was detected in most of them. The most common detected virulence genes were included efbA, ace, and gelE. RAPD-PCR and PFGE analyses showed the same patterns of molecular fingerprints between paired of the isolates in 26.9% and 15.8% of the patients, respectively. Similarity of E. faecalis strains between the urine and feces samples confirmed the occurrence of endogenous
ABSTRACT All enterococci produce a complex polysaccharide called the enterococcal polysaccharide antigen (EPA). This polymer is required for normal cell growth and division and for resistance to cephalosporins and plays a critical role in host-pathogen interaction. The EPA contributes to host colonization and is essential for virulence, conferring resistance to phagocytosis during the infection. Recent studies revealed that the
TY - JOUR. T1 - Incidence of hemolysin, gelatinase, and aggregation substance among enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons. AU - Coque, Teresa M.. AU - Patterson, Jan E.. AU - Steckelberg, James M.. AU - Murray, Barbara E.. PY - 1995/5. Y1 - 1995/5. N2 - The presence of hemolysin, gelatinase, and aggregation substance (by use of a probe known to hybridize to most pheromone-responsive plasmids) was determined in 192 isolates of Enterococcus faecalis from patients with endocarditis or other infections and fecal isolates from hospitalized patients or healthy volunteers, and in 86 non-E. faecalis isolates. Hemolysin was more common in nonendocarditis clinical isolates and in hospital fecal isolates (37% and 31%, respectively) than among endocarditis and community fecal isolates (16% and 20%, respectively). Gelatinase and aggregation substance, respectively, were found in 54% and 52% of isolates from ...
BACKGROUND: Studies on transmission of Enterococcus faecalis among chickens during hatch have not been carried out so far. Information about vertical transmission and subsequent spreading and colonization of the cloacal mucosa through cloacal drinking during hatch are important to understand the epidemiology of E. faecalis infections. In the present investigation vertical transmission and subsequent spreading and colonization of the cloacal mucosa of chickens by E. faecalis through cloacal drinking were examined. METHODS: Two different batches of layer chickens originating from 45 weeks old Brown and White Lohmann parents, respectively from the same farm were sampled in the hatcher. Isolates were confirmed to be E. faecalis by polymerase chain reaction (PCR) and further by multilocus sequence typing (MLST) to state their population structure and comparison made to sequence types previously obtained from chicken. RESULTS: A total of 480 chickens were swabbed from the cloacae just after hatch ...
TY - JOUR. T1 - Immunological Characterization of Pheromone-induced Proteins Associated with Sexual Aggregation in Enterococcus faecalis. AU - Nakayama, Jiro. AU - Watarai, Hiroshi. AU - Isogai, Akira. AU - Suzuki, Akinori. PY - 1992/1/1. Y1 - 1992/1/1. N2 - Sexual aggregation involved in conjugative transfer of the Enterococcus faecalis plasmids pPDl and pADl is enhanced by sex pheromones cPDl and cADl, respectively, which are excreted from recipient cells. PD78 (78kDa) and AD74 (74kDa) were detectable on the surface of donors harboring pPDl and pAD1, respectively, at the time of cell aggregation. The proteins PD78 and AD74 were purified and used to raise anti-PD78 and anti-AD74 antibodies. The antibodies blocked the sexual aggregation and the plasmid transfer. Anti-AD74 antibody reacted to both 153 kDa proteins extracted from cPDl and cADl-induced donor cells after lysozyme digestion of cell wall. Pheromone-induced synthesis of PD78 and AD74, when both plasmids were present in the same cell, ...
Naturally occurring in the human gut, enterococci bacteria can lead to infections including bloodstream and urinary tract infections, infections of surgical sites, and endocarditis - infection of the heart valves.. Researchers examined the genomes of the bacteria to analyze samples from an early outbreak of bacteremia in patients in a Wisconsin hospital between 1984 and 1988 that was caused by multidrug-resistant Enterococcus faecalis in order to learn how they adapted to existence in the hospital and transmission from one patient to another. By going back to the early days of the antibiotic resistance problem, Dr. Van Tyne, Dr. Gilmore, and colleagues were able to see that Enterococcus faecalis entering into the bloodstream first turn on an unusual pathway that allows the microbe to make a new substance that helps to shore up its cell wall. This makes the bacterium more able to resist being killed by white blood cells, and also by antibiotics of the penicillin class that attack the bacterial ...
Enterococcus faecalis bacteria. Coloured scanning electron micrograph (SEM) of a clump of Enterococcus faecalis bacteria. This Gram-positive coccus (round bacterium) is a normal component of the human gastrointestinal tract, but under certain conditions can cause diseases in humans, especially hospital patients with reduced resistance to infections. - Stock Image C026/7490
Lien vers Pubmed [PMID] - 23700464. PLoS ONE 2013;8(5):e64218. Enterococcus faecalis (E. faecalis) has become a major leading cause of nosocomial endocarditis. Treatment of such infections remains problematic and new therapeutic options are needed. Nine E. faecalis strains were tested: six obtained from patients presenting endocarditis, one with isolated bacteremia, and two reference strains. Antibiotics included daptomycin, alone or in combination, linezolid, tigecycline, rifampicin, gentamicin, teicoplanin, ceftriaxone and amoxicillin. Time-kill studies included colony counts at 1, 4 and 24 h of incubation. Significant bactericidal activity was defined as a decrease of ≥3log10CFU/ml after 24 h of incubation. Antibiotics were tested at a low (10(6) CFU/ml) and high (10(9) CFU/ml) inoculum, against exponential- and stationary-phase bacteria. We also performed time kill studies of chemically growth arrested E. faecalis. Various pH conditions were used during the tests. In exponential growth ...
Enterococcus faecalis ATCC ® 29212™ Designation: TypeStrain=False Application: Food testing Control strain ref   ref Evaluation of Mueller-Hinton agar ref Media testing ref   ref   ref   ref   ref   ref   ref   ref Quality control ref Quality control strain ref   ref   ref   ref   ref Susceptibility disc testing ref   ref   ref Susceptibility testing ref   ref   ref   ref   ref   ref   ref   ref   ref   ref Quality control strain for API, bioMerieux VITEK, IDS, Micro-Media, MicroScan, and Sensititre products
Klibi, Naouel and Ben Slama, Karim and Sáenz, Yolanda and Masmoudi, Afef and Zanetti, Stefania Anna Lucia and Sechi, Leonardo Antonio and Boudabous, Abdellatif and Torres, Carmen (2007) Detection of virulence factors in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolates from a Tunisian hospital. ...
The stringent response is a global bacterial response to stress that is mediated by accumulation of the alarmone (p)ppGpp. In this study, treatment with mupirocin was shown to induce high levels of (p)ppGpp production in Enterococcus faecalis, indicating that this nosocomial pathogen can mount a classic stringent response. In addition, (p)ppGpp was found to accumulate in cells subjected to heat shock, alkaline shock, and inhibitory concentrations of vancomycin. Sequence analysis of the E. faecalis genome indicated that (p)ppGpp synthesis is catalyzed by the bifunctional synthetase/hydrolase RelA and the RelQ small synthase. The (p)ppGpp profiles of ΔrelA, ΔrelQ, and ΔrelAQ strains revealed that RelA is the major enzyme responsible for the accumulation of (p)ppGpp during antibiotic or physical stresses, while RelQ appears to be responsible for maintaining basal levels of alarmone during homeostatic growth. Compared to its parent, the ΔrelA strain was more susceptible to several stress ...
Thirty-seven nonhemolytic/nonbacteriocinogenic mutations in Enterococcus (Streptococcus) faecalis plasmid pAD1 were generated by Tn917 insertion. All were found to belong to one of two complementation classes. Each class of mutants secreted either hemolysin/bacteriocin (Hly/Bac) component A or L into the culture medium. DNA encoding Hly/Bac was cloned in Escherichia coli in which both components of the hemolysin were expressed individually and collectively. The region encoding components A and L was further defined by deletion analysis and physically mapped. A total of approximately 8.4 kilobases of pAD1 DNA were observed to be required for hemolysin expression. Hly/Bac activity of the wild-type and the inactive L substance was observed to be heat stable. Active Hly/Bac resulting from incubating separately secreted components A and L was also found to be heat stable. The results indicate that component A activates component L and that activated component L possesses the Hly/Bac activity. ...
TY - JOUR. T1 - Antibacterial effects of sarang semut (Myrmecodia Pendans) fractions using three different solvents toward enterococcus faecalis CPS2. AU - Binartha, Ciptadhi Tri Oka. AU - Suprastiwi, Endang. AU - Kurnia, Didik. AU - Margono, Anggraini. AU - Artiningsih, Dewa Ayu Nyoman Putri. PY - 2020/3. Y1 - 2020/3. N2 - Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane), semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2. Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groups of solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using a phytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group ...
Pyruvate Broth Test Objectives To determine the ability of an organism to utilize pyruvate. It aids in the differentiation between Enterococcus faecalis and Enterococcus faecium. Principle In the identification of microorganisms, the widely used method is the ability of bacteria to form organic compounds by metabolizing certain carbohydrates and related compounds. Pyruvate broth is a carbohydrate-free, … Read more Pyruvate Broth Test. ...
Canadian researchers have published a study assessing the antimicrobial resistance and virulence of genotypes of Enterococcus faecalis, taken from samples taken from a commercial pork processing plant. They conclude that the pork meat appears to be arriving at the plant free of E. faecalis, suggesting that contamination occurs during processing.
Replication Data for publication: Enterococcus faecalis adapts to antimicrobial conjugated oligoelectrolytes by lipid rearrangement and differential expression of membrane stress response genes. Includes: RNA sequencing data: RAW reads RNA sequencing data: non rRNA reads RNA sequencing data: extracted counts See RNAseq-readme.txt for file description.
This issue profiles a bacteriophage that aids efforts to destroy Enterococcus Faecalis bacteria, preventing salmonellosis through the usage of lytic bacteriophages, and development of multiple phage therapy products for infectious diseases .
While multiple alpha 1-2-mannosidases are necessary for glycoprotein N-glycan maturation in vertebrates, a single bacterial alpha 1-2-mannosidase can be sufficient to cleave all alpha 1-2-linked mannose residues in host glycoprotein N-glycans. We report here the characterization and crystal structure of a new alpha 1-2-mannosidase (EfMan-I) from Enterococcus faecalis, a Gram-positive opportunistic human pathogen. EfMan-I catalyzes the cleavage of alpha 1-2-mannose from not only oligomannoses but also high-mannose-type N-glycans on glycoproteins. Its 2.15 angstrom resolution crystal structure reveals a two-domain enzyme fold similar to other CAZy GH92 mannosidases. An unexpected potassium ion was observed bridging two domains near the active site. These findings support EfMan-I as an effective catalyst for in vitro N-glycan modification of glycoproteins with high-mannose-type N-glycans ...
Intestinal commensals are potential important contributors to the etiology of sporadic colorectal cancer, but mechanisms by which bacteria can initiate tumors remain uncertain. Herein, we describe mechanisms that link Enterococcus faecalis, a bacterium known to produce extracellular superoxide, to t …
Isolated Enterococcus faecalis YZ 66 strain shows ability to decolorize various industrial dyes among which, it showed complete decolorization and degradation of toxic, sulfonated recalcitrant diazo dye Direct Red 81 (50 mg/L) within 1.5 h of incubation under static anoxic condition. The optimum pH …
Enterococcus faecalis in the largest biology dictionary online. Free learning resources for students covering all major areas of biology.
Enterobacter, Serratia, Proteus, Pseudomonas, Providencia, Morganella, Staphylococci, Streptococci, Enterococcus. amoxicillin-clavunic acid & ampicillin.. . and from CO 2 sensitive. or markers that confer antibiotic resistance such as ampicillin. but not limited to, Bacillus, Clostridium, Enterococcus.Indiquons que rarement lhydroxyquinoléine peut entraîner des neuropathies sensitives ainsi que des. Ampicilline: 1961:. enterococcus faecalis, sont deux.Létat lentérocoque а les décla rations des. indicating that this to sensitive. L on si est antibiothérapie par voie ampicilline.52 52 Days Log CFU/g of feces Ampicilline dans leau de boisson. Enterococcus faecalis (80%) et. veterinary medicine MIC 90 %sensitive Chicken µg/mL Cattle µg.selection of positive transformation events the ampr gene coding for ampicillin resistance. As a very sensitive staining method silver staining according to Blum.The LE1 Bacteriophage Replicates as a Plasmid within. and ampicillin, tobramycin,. but were ...
What types of infections does VRE cause?VRE can live in the human intestines and female genital tract without causing disease (often called colonization). However, sometimes it can cause infections of the urinary tract, the bloodstream, or of wounds associated with catheters or surgical procedures.Who is at risk for infection?
Enterococci can be used in the food industry as starter or probiotic cultures. However, enterococci are also implicated in severe multi-resistant nosocomial infections. In this study, the prevalence of enterococci in selected Brazilian foodstuffs (raw and pasteurized milk, meat products, cheeses and vegetables) was evaluated. Phenotypic and PCR protocols were used for species identification. Tests for production of gelatinase, haemolysin, bacteriocin and bile salt hydrolysis were done with all enterococci isolates, whereas molecular determination of virulence markers (genes esp, gel, ace, as, efaA, hyl and cylA) and antibiotic resistance was checked only for Enterococcus faecium and Enterococcus faecalis isolates. The antibiotic-resistant isolates were assayed for biofilm formation and adhesion to mammalian cells. From the 120 food samples analyzed, 52.5% were positive for enterococci, meat and cheese being the most contaminated. E. faecium was the predominant species, followed by E. faecalis, ...
Resistance to vancomycin among enterococci is an emerging health problem worldwide. In Sudan, limited data is present about its molecular epidem..
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Enterococci are gram-positive bacteria that are normal inhabitants of the alimentary tract of humans and other animals. They have been recognized as a cause of infective endocarditis for more than a century (17) and have gained prominence over the last two decades as being among the most common pathogens found in hospital-acquired infections, including urinary tract infections, bloodstream infections, and surgical-site infections (27). The increasing importance of enterococci as nosocomial pathogens can be attributed in part to intrinsic and acquired antibiotic resistance (17, 26). Treatment of multidrug-resistant enterococcal infections poses a significant challenge to clinicians (4, 8), and the potential of these organisms to serve as a reservoir for antibiotic resistance genes is of great concern (6, 20, 21). Despite increasing recognition of the clinical importance of enterococcal infections, their pathogenic mechanisms are not well understood (11).. We have recently developed a novel model ...
Case presentation: Four case reports are presented in order to emphasize the possible clinical implications of genitourinary bacterial infections on male infertility. The first patient had two bacterial strains isolated from the semen culture, Escherichia coli, and then Enterococcus faecalis. The antibiotic treatment was not effective. The second patient had a semen culture positive with Enterococcus faecalis. The treatment was successful: the bacteria were eradicated and the patient was able to conceive a baby. Enterococcus faecalis was also identified in the third and the fourth case. These patients were able to conceive, despite the different clinical management strategies of the cases ...
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Salah satu penyebab kegagalan perawatan endodontik adalah mikroba yang bertahan di dalam sistem saluran akar, termasuk Enterococcus faecalis. Senyawa yang terdapat pada ekstrak etanol rimpang jahe yang terdiri dari gingerol, paradol, shogaol, zingerone dan minyak atsiri diduga merupakan golongan senyawa bioaktif yang dapat menghambat pertumbuhan mikroba. Tujuan penelitian adalah mengetahui efek antimikroba ekstrak etanol rimpang jahe (EERJ) terhadap Enterococcus faecalis. Penelitian bersifat eksperimental laboratorik. Penelitian ini dilakukan dengan menggunakan kertas cakram steril yang ditetesi ekstrak etanol rimpang jahe (Zingiber offcinale Rosc.) dengan konsentrasi 5%, 10%, 20% dan 40% dan klorheksidin 0,2% sebagai kontrol positif serta kertas cakram steril yang ditetesi akuades steril sebagai kontrol negatif pada medium Mueller-Hinton Agar (MHA) yang telah dibiakkan bakteri Enterococcus faecalis. Cakram diinkubasi pada suhu 37o selama 24 jam. Data yang diukur adalah diameter zona hambat ...
TY - JOUR. T1 - Structural insights into the alanine racemase from Enterococcus faecalis. AU - Priyadarshi, Amit. AU - Lee, Eun Hye. AU - Sung, Min Woo. AU - Nam, Ki Hyun. AU - Lee, Won Ho. AU - Kim, Eunice EunKyeong. AU - Hwang, Kwang Yeon. PY - 2009/7/1. Y1 - 2009/7/1. N2 - Alanine racemase (AlaR) is a bacterial enzyme that belongs to the fold-type III group of pyridoxal 5′-phosphate (PLP)-dependent enzymes. AlaR catalyzes the interconversion between l- and d-alanine, which is important for peptidoglycan biosynthesis. This enzyme is common in prokaryotes, but absent in eukaryotes, which makes it an attractive target for the design of new antibacterial drugs. Here, we report the crystal structures of both the apoenzyme and the d-cycloserine (DCS) complex of AlaR from the pathogenic bacterium Enterococcus faecalis v583, at a resolution of 2.5 Å. DCS is a suicide inhibitor of AlaR and, as such, serves as an antimicrobial agent and has been used to treat tuberculosis and urinary tract ...
Enterococcus faecalis is recognized as one of the leading pathogens causing nosocomial infections. Here we report a draft genome sequence of Enterococcus faecalis SK460, isolated from a chronic diabetic foot ulcer patient. This strain exhibits various biofilm-associated genes, virulence genes, and antibiotic-resistance genes related to aminoglycoside, macrolide, and tetracycline resistance.. ...
TY - JOUR. T1 - Effect of Smear Layer and Chlorhexidine Treatment on the Adhesion of Enterococcus faecalis to Bovine Dentin. AU - Yang, Seung Eun. AU - Cha, Jeong Heon. AU - Kim, Eui Seong. AU - Kum, Kee Yeon. AU - Lee, Chang Young. AU - Jung, Il Young. N1 - Funding Information: This study was supported in part by grants from College of Dentistry, Yonsei University.. PY - 2006/7. Y1 - 2006/7. N2 - The aim of this in vitro study was to determine the effects of a smear layer and chlorhexidine (CHX) treatment on the adhesion of Enterococcus faecalis to bovine dentin. Forty dentin blocks from bovine incisors were prepared and randomly divided into four groups of 10 each. The blocks in group 1 were placed in sterile saline for 5 minutes, while those in group 2 were treated with 17% EDTA for 5 minutes. The blocks in group 3 were placed in 2% CHX for 7 days. The blocks in group 4 were treated with 17% EDTA for 5 minutes, and then placed in 2% CHX for 7 days. All the blocks were immersed in a suspension ...
CHARACTERIZATION OF AZO DYE REDUCTION IN ENTEROCOCCUS FAECALIS By SUMIT PUNJ Bachelor of Science University of Mumbai, Mumbai, India 1998 Master of Science University of Mumbai Mumbai, India 2000 Submitted to the Faculty of the Graduate College of the Oklahoma State University in partial fulfillment of the requirements for the Degree of DOCTOR OF PHILOSOPHY July, 2008 ii CHARACTERIZATION OF AZO DYE REDUCTION IN ENTEROCOCCUS FAECALIS Dissertation Approved: Dr. Gilbert H. John Dissertation Adviser Dr. Robert L. Burnap Dr. Rolf A. Prade Dr. Babu Z. Fathepure Dr. Carol L. Bender Dr. A. Gordon Emslie Dean of the Graduate College iii ACKNOWLEDGMENTS I would like to express my gratitude to my adviser, Dr. Gilbert H. John who has been very patient and understanding throughout my years in the program. He has given me the opportunity to develop and execute my ideas and has always been very encouraging. I sincerely thank each one of my committee members; Dr. Robert Burnap, Dr. Rolf Prade, Dr. Babu ...
TY - JOUR. T1 - Comparison of antibacterial efficacy between xanthorrhizol (Curcuma xanthorrhiza roxb.) and chlorhexidine 2% against enterococcus faecalis clinical isolate biofilm. AU - Permitasari, Riza. AU - Nazar, Kamizar. AU - Meidyawati, Ratna. AU - Prasetyanti, Rizka Eka. PY - 2020/7. Y1 - 2020/7. N2 - Objective: In root canal treatments, chlorhexidine (CHX) is widely used for irrigation and is effective in killing Enterococcus faecalis. CHX is a synthetic chemical and is toxic to host cells; therefore, natural or herbal irrigation solutions, which are safer but still effective, are necessary. The aim of this study is to analyze the effect of xanthorrhizol (XNT) derived from Curcuma xanthorrhiza Roxb. on E. faecalis clinical isolate biofilm formation (0.5%, 0.75%, 1%, 1.25%, and 1.5%). Methods: The MTT assay and total plate count were performed for assessing the effectiveness of herbal ingredients, while CHX (2%) was used as a positive control. Data were analyzed using one-way ANOVA and ...
International Scholarly Research Notices is a peer-reviewed, Open Access journal covering a wide range of subjects in science, technology, and medicine. The journals Editorial Board as well as its Table of Contents are divided into 108 subject areas that are covered within the journals scope.
Inflammatory bowel diseases (IBDs), which afflict 1.6 million people in the United States, are chronic, relapsing and immune-mediated intestinal disorders caused in part by aggressive T-cell-mediated immune responses to intestinal microbes in genetically susceptible individuals. However, relatively little is known about how intestinal inflammation affects the function of gut microbiota. We show that Il10-/- mice colonized with a simplified, defined microbial consortium that includes Enterococcus faecalis develop immune-mediated colitis. Transcription of two putative E. faecalis phosphotransferase systems (PTS) that import sugars into the bacterium is upregulated in colitis. We identify gluconate, ribose, and glucosamine as potential substrates for these PTS. The presence of these PTS is associated with altered intestinal microbial ecology and worsened colitis, therefore suggesting a role for E. faecalis sugar metabolism in colitis development. This work highlights the complexity of ...
The success of Enterococcus faecium and E. faecalis evolving as multi-resistant nosocomial pathogens is associated with their ability to acquire and share adaptive traits, including antimicrobial resistance genes encoded by mobile genetic elements (MGEs). Here, we investigate this mobilome in successful hospital associated genetic lineages, E. faecium sequence type (ST)17 (n=10) and ST78 (n=10), E. faecalis ST6 (n=10) and ST40 (n=10) by DNA microarray analyses. The hybridization patterns of 272 representative targets including plasmid backbones (n=85), transposable elements (n=85), resistance determinants (n=67), prophages (n=29) and clustered regularly interspaced short palindromic repeats (CRISPR)-cas sequences (n=6) separated the strains according to species, and for E. faecalis also according to STs. RCR-, Rep_3-, RepA_N- and Inc18-family plasmids were highly prevalent and with the exception of Rep_3, evenly distributed between the species. There was a considerable difference in the replicon profile
Conjugative transfer of the Enterococcus faecalis plasmid pCF10 is induced by the peptide pheromone cCF10 when recipient-produced cCF10 is detected by donors. cCF10 is produced by proteolytic processing of the signal sequence of a chromosomally encoded lipoprotein (CcfA). In donors, endogenously produced cCF10 is carefully controlled to prevent constitutive expression of conjugation functions, an energetically wasteful process, except in vivo, where endogenous cCF10 induces a conjugation-linked virulence factor. Endogenous cCF10 is controlled by two plasmid-encoded products; a membrane protein PrgY reduces pheromone levels in donors, and a secreted inhibitor peptide iCF10 inhibits the residual endogenous pheromone that escapes PrgY control. In this study we genetically determined the amino acid specificity determinants within PrgY, cCF10, and the cCF10 precursor that are necessary for cCF10 processing and for PrgY-mediated control. We showed that amino acid residues 125 to 241 of PrgY are ...
As a popular medicine for probiotic therapy to treat with patients with diarrhea, the safety of Shin Biofermin S is paramount. Therefore, in the present study, the species of the SBS-1 strain isolated from a commercial medicine (Shin Biofermin S) was determined via genome sequencing. Species identification is important for evaluating safety and is favorable for formulating therapeutic strategies. The scientific names of E. faecium have been changed several times. Streptococcus faecalis and Streptococcus faecium were separate until 1984, when DNA-DNA and DNA-rRNA hybridization tests revealed that they belonged to Enterococcus instead (5). Bergeys Manual of Systematic Bacteriology Volume 3: The Firmicutes also demonstrated this result (6), such that Streptococcus faecalis was renamed Enterococcus faecalis. In the food and health industry, E. faecium and E. faecalis have been used to improve the intestinal environment by regulating its inflammatory status and the gut microflora (2). However, the ...
Enterococci are isolated from 10% to 15% of patients with endocarditis (1-3) and rank as the third commonest cause of endocarditis, behind viridans streptococci and Staphylococcus aureus (3). These group D streptococci (Lancefield classification) differ physiologically from most other streptococci by their ability to grow in media containing 40% bile and to cleave esculin. Enterococci are distinguished from nonenterococcal group D organisms (that is, S. bovis or S. equinus) by their growth in broth containing 6.5% sodium chloride. Enterococcal endocarditis is usually caused by S. faecalis and rarely by S. faecium or S. durans.. Therapy for patients with enterococcal endocarditis ...
The prevalence and diversity of antibiotic resistant enterococci populations in samples collected four times from urban sewage treatment plant in Tehran, Iran between June 2005 and July 2006 were studied. Filtered samples were grown on mEnterococci medium containing 4 μg/ml vancomycin after which the enterococci isolates were identified to the species level. All strains were then tested for their resistance against nine antibiotics. Of the 131 isolates, 98 (75%) isolates were identified as Enterococcus gallinarum, followed by 24 (18%) and 9 (7%) for E. faecium and E. casseliflavus, respectively. All E. gallinarum isolates carried vanC1 gene with 64 (65%) and 14 (14%) isolates concomitantly harboured either vanA or vanB gene, respectively. Some E. casseliflavus concomitantly harboured vanA and vanC2 or vanB and vanC2. Typing the total enterococci isolates with a high resolution biochemical fingerprinting method showed a high diversity (D i = 0.91). We have shown by biochemical fingerprinting the
We showed in vitro and in experimental endocarditis that glycopeptide resistance in enterococci did not affect the activity of GAR-936, as demonstrated by similar activities of the drug against two isogenic strains differing by their glycopeptide susceptibility. This result, which was already reported by others (11; S. M. Mikels, E. B. Lenoy, W. Allen, S. Compton, and W. J. Weiss, Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. 135, 1998), could be anticipated from the absence of relation between mechanisms of resistance to glycopeptides and to tetracyclines. GAR-936 was not less active against a tetracycline-resistant VanA type E. faecium isolate than against a tetracycline-susceptible VanA type E. faecalis strain, showing that the ability of GAR-936 to overcome the mechanisms responsible for tetracycline resistance is relevant in vivo. Since glycopeptide resistance is often associated to multidrug resistance, including tetracycline resistance (9), our results suggest that ...
Mikrobiologisk virkningsspektrum. Virker baktericidt på grampositive kokker (Streptococcus pneumoniae (pneumokokker), Streptococcus pyogenes (β-hæmolytiske streptokokker, gruppe A), Streptococcus agalactiae (β-hæmolytiske streptokokker, gruppe B), Enterococcus faecalis og de fleste α-hæmolytiske streptokokker samt på de Staphylococcus aureus, som ikke danner penicillinase), grampositive stave (Listeria monocytogenes, Clostridium spp., Bacillus anthracis, Corynebacterium spp. og Actinomyces spp.), gramnegative diplokokker (Neisseria meningitidis og nogle stammer af Neisseria gonorrhoeae), visse gramnegative stave som Haemophilus influenzae, Proteus mirabilis, E. coli, Helicobacter pylori, Pasteurella spp. og Capnocytophaga spp. og endelig over for Treponema pallidum og Borrelia burgdorferi. ...
Objective: To determine if the synthetic peptide BM2 has antimicrobial activity against mono-species biofilms of common endodontic pathogens.. Methods:. Strains of the pathogenic bacteria Enterococcus faecalis JH2-2, Streptococcus gordonii DL1, and Streptococcus mutans NG8 and the fungal pathogen Candida albicans ATCC 10261 were grown from glycerol stocks in closed tubes containing Todd Hewitt Broth (THB) or Tryptone Soy Broth (TSB) for 24 hours. Mono-species biofilms were prepared in 96-well plates for 72 hours before exposure to antimicrobial agents. Dilution series of the synthetic antimicrobial peptide BM2 (D-NH2-RRRFWWFRRR-CONH2) and the widely used endodontic antimicrobial agents sodium hypochlorite (NaOCl) and saturated calcium hydroxide (Ca(OH)2) were prepared as aqueous solutions. The efficacy of BM2 (10 µg/mL, 20 µg/mL and 40 µg/mL), NaOCl (2,500 µg/mL, 5,000 µg/mL and 10,000 µg/mL) and saturated Ca(OH)2 at limiting cell growth and possibly causing biofilm disruption was measured ...
Histopathological changes induced in an animal model by potentially pathogenic Enterococcus faecalis strains recovered from ready-to-eat food outlets in Osun State, Nigeria Adetunji Kola Olawale,1,2 Oluwole Moses David,2,3 Adekemi Olubukunola Oluyege,2 Richard Temitope Osuntoyinbo,4 Solomon Anjuwon Laleye,5 Oladiran Famurewa,2 1Department of Applied Sciences, Osun State Polytechnic, Iree, 2Department of Microbiology, University of Ado-Ekiti, Ado-Ekiti, Nigeria; 3Phytomedicine Research Centre, Department of Botany, University of Fort Hare, Alice, South Africa; 4Department of Microbiology, Waterford Regional Hospital, Waterford, Republic of Ireland; 5Department of Microbiology, Adekunle Ajasin University, Akungba-Akoko, Nigeria Abstract: Enterococci have been implicated as an emerging important cause of several diseases and multiple antibiotic resistance. However, there is little information about the prevalence of pathogenic and/or antibiotic-resistant Enterococcus faecalis in ready-to-eat foods in
Bacteria are the primary cause of pulp and periapical pathology. They gain entry into the tooth through exposed dentinal tubules. Although bacteria have been shown to invade these dentinal tubules, the cell interactions or processes involved in invasion are still not clear. This study will look at one possible process, contact guidance/thigmotropism, that has been shown in plants, some animal cells and with a known oral microbe Candida albicans.. Aim: To determine contact guidance characteristics by endodontic pathogens Streptococcus mutans NG8, Streptococcus gordonii DL-1, Enterococcus faecalis JH2-2 and C. albicans ATCC 10261 on micro-grooved polymethylmethacrylate (PMMA) discs. In addition, to determine the effect an amino acid sequence in collagen type I (Glycine-Proline-Alanine) has on growth characteristics. Methods: Micro-grooves of various dimensions (2 µm x 2 µm x 2 µm (Width Depth Spacing), 2 µm x 2 µm x 4 µm (WDS), 4 µm x 2 µm x 2 µm (WDS) for bacteria and 4 µm x 4 µm x 2 ...
Summary: Biochemical, menaquinone, fatty acid and DNA analyses were conducted on a number of streptococci of serological group D. The results indicate that S. faecalis, S. faecium, S. casseliflavus and taxa previously designated S. avium, S. durans and S. faecalis var. malodoratus are distinct species. Strains previously labelled S. faecium var. mobilis were shown to be identical with S. casseliflavus. The results also indicate that some group D streptococci recently isolated from chickens constitute a new species.
Hospital-acquired infections caused by enterococci have increased dramatically since the 1970s. Many nosocomial enterococcal bloodstream infections are associated with medical devices such as central venous catheters. The ability to form biofilm on medical devices is a potential virulence trait that may allow enterococci to cause infections in the expanding population of patients managed with such devices. In this study, the hypothesis that increased ability to form biofilm in vitro is associated with medical-device-related infection in vivo was tested. A microplate assay was employed to assess biofilm-forming characteristics of enterococci in 0.9 % (w/v) sodium chloride, an oligotrophic environment, and BHI, a nutrient-rich environment. Results were compared in isolates from different sources of infection. One hundred and nine enterococcal bloodstream isolates were assayed. Biofilm formation on microplates was demonstrated by all Enterococcus faecalis isolates and 16/38 (42 %) Enterococcus faecium
SUMMARY: Although the Gram-positive bacterium Streptococcus pyogenes (group A streptococcus) has been considered an extracellular pathogen which adheres to human mucosal epithelium, the streptococcus possesses invasive capacity for cultured human epithelial cells. This study provides genetic and functional evidence supporting the conclusion that protein F is capable of mediating entry of S. pyogenes into HeLa cells. Using 111916 insertion mutagenesis or an isogenic 5. pyogenes strain with a defined mutation in the gene encoding protein F (prtF), it was observed that the invasive capacity was affected by the levels of surface-exposed protein F, but not by those of M protein. In addition, heterologous expression of protein F on Enterococcus faecalis conferred upon the bacteria an efficient invasive phenotype. Several assays demonstrated that both the fibronectin-binding domains of protein F, UR and RD2, were involved in host-cell invasion. In addition, coinfection experiments of HeLa cells with 5.
18 hours) Intrapartum fever (>38C)[vague] Prior GBS affected infante[vague] GBS bacteriuria during this pregnancy This protocol results in treatment[vague] of 15-20% of pregnant women and prevention of 65-70% of cases of early onset GBS sepsis. This group includes S. equi, which causes strangles in horses, and S. zooepidemicus-S. equi is a clonal descendent or biovar of the ancestral S. zooepidemicus-which causes infections in several species of mammals, including cattle and horses. S. dysgalactiae is also a member of group C, beta-haemolytic streptococci that can cause pharyngitis and other pyogenic infections similar to group A streptococci. Many former group D streptococci have been reclassified and placed in the genus Enterococcus (including E. faecalis, E. faecium, E. durans, and E. avium). For example, Streptococcus faecalis is now Enterococcus faecalis. E. faecalis is sometimes alpha hemolytic and E. faecium is sometimes beta hemolytic. The remaining nonenterococcal group D strains ...
Extratos seco, fresco e glic lico de Zingiber officinale foram obtidos para avaliar suas a es por meio de ensaio de sobreviv ncia em G. mellonella contra infec o por Enterococcus faecalis. Oitenta larvas foram divididas em: 1) Suspens o de E. faecalis (controle); 2) E. faecalis + extrato fresco de Z. officinale (FEO); 3) E. faecalis + extrato seco de Z. officinale (DEO); 4) E. faecalis + extrato glic lico de Z. officinale (GEO); 5) Solu o tamp o fosfato salina (PBS). Para o grupo de controle, 5 L de in culo de suspens o padronizada (107 c lulas/mL) de E. faecalis (ATCC 29212) foi injetado na ltima proleg esquerda de cada lagarta. Para os grupos com tratamento, ap s a inje o de E. faecalis, os extratos foram injetados na ltima proleg direita. Ap s as inje es, as lagartas foram armazenadas a 37 C e o n mero de animais mortos foi registrado diariamente em 168 h (7 dias) para analisar a curva de sobreviv ncia. As lagartas foram consideradas mortas quando elas n o mostraram qualquer movimento ap s o ...
Mr. Perrault has spent over 10 years working in a laboratory setting with experience in research and development, product development, quality control and manufacturing. He graduated from the University of Minnesota, Morris with a B.A. in Biology. After college he worked at the University of South Dakotas Medical School. As a research scientist he was introduced to microbiology and molecular biology assays and techniques while performing research on Enterococcus faecalis. In 2007 he started out as a laboratory technician at the University of Minnesotas Schulze Diabetes Institute performing more than 800 islet cell isolations for R/D, xenotransplantation, auto-transplantation and allo-transplantation. In 2012 he became the quality control laboratory manager for the human and porcine islet isolation program responsible for the release of clinical islet isolation products for human transplantation. In 2013 he joined Miromatrix Medical and participated in ramping up the acellular product line as ...
Curated}} {{Biorealm Genus}} [[Image:enterococcus2.jpg,frame,right,Enterococcus cultured on agar. From the [http://w3.ouhsc.edu/enterococcus/ University of Oklahoma.]]] ==Classification== ===Higher order taxa:=== Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus ===Species:=== Enterococcus faecalis, Enterococcus faecium {, , height=10 bgcolor=#FFDF95 , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Undef&id=1350&lvl=3&keep=1&srchmode=1&unlock Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid1350%5BOrganism:exp%5D Genome] ,} ==Description and Significance== Enterococci are regular inhabitants of the bowel. The genome of E. faecalis is more than 25% exogenously acquired DNA. Enterococci are the leading cause of hospital-acquired secondary infections. ==Genome Structure== The genome of Enterococcus faecalis V583 was recently sequenced. The main chromosome is 3,218,031 ...
Three Streptococcal strains: S. milleri P213, S. milleri P35 and S. milleri B200 and three enterococcal strains: E. faecalis 123, E. faecalis 126 and E. faecium were used to test for vancomycin resistance. Two strains were used as reference strains that were already characterized as vancomycin resistant. E. faecium BM4147 was used as a VanA control and E. faecalis ATCC was used as a VanB control. Susceptibility of each strain to this antibiotic was tested by disk-diffusion assay and the MIC values for the strains were found to be between 5 - 10 ug/ml and for the VanA control, the MIC was , 64 ug/ml and for the VanB control was 32 ug/ml. These MIC values indicate that S. milleri P213, S. milleri P35, S. milleri B200, E. faecalis 123, E. faecalis 126, and E. faecium are all of the VanC phenotype. All strains were tested for lysis by means of addition of vancomycin (10 ug/ml) to the bacterial cultures. Lytic curves were constructed and the VanB control was found to be most autolytic upon addition ...
THE ANTIBACTERIAL potential of Ziziphus- spina christi as methanol and ethanol extracts, of bark, fruit, roots, seeds and leaves, were evaluated against seven pathogenic bacterial strains using agar well diffusion technique: The used strains were Pseudomonas aeruginosa (ATCC 278223), Enterobacter cloacae (ATCC 13047), Enterobacter aerogenes (ATCC 13084), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13888), Enterococcus faecalis (ATCC 29212) and Methicillin-resistant Staphylococcus aureus (MRSA, ATCC 43300). The antibiotic erythromycin was used as positive control. The presence of phytochemical compounds in the extracts was determined qualitatively, the functional bioactive groups were characterized by FTIR and the presence of bioactive elements was characterized by XRF. Antibacterial activity against the used bacterial strains was assessed by determining the minimal inhibitory and bactericidal concentrations (MIC and MBC) assays. All the used Gram negative and positive bacteria were
MONDAY, July 8, 2019 (HealthDay News) - The prevalence of infective endocarditis (IE) is about 26 percent among patients with Enterococcus faecalis bacteremia, according to a study published in the July 16 issue of the Journal of the American College of Cardiology. Anders Dahl, M.D., Ph.D., from Herlev Gentofte University Hospital in Copenhagen, Denmark, and colleagues examined the prevalence of IE in patients with E. faecalis bacteremia in a prospective multicenter study conducted in consecutive patients from Jan. 1, 2014, to Dec. 31, 2016. Data were included for 344 patients with E. faecalis bacteremia who were examined using echocardiography; 74 percent of the cases had transesophageal echocardiography (TEE). The researchers found that definite endocarditis was diagnosed in 90 patients, yielding a prevalence of 26.1 ± 4.6 percent. Prosthetic heart valve, community acquisition, three or more positive blood culture bottles, unknown portal of entry, monomicrobial bacteremia, and ...
The success of root canal treatment is mainly based on the complete eradication of micro organisms by instrumentation and disinfection protocols followed during the procedure Due to anatomical complexities achieving long term success would be difficult because these areas have limited access to instruments and irrigants Hence application of intracanal medicament has been considered as an important step to reduce the persistent bacteria inside the root canal Since the routine intracanal medicaments could not penetrate into the dentinal tubules the role of Nantotechnology was explored in Endodontics The field of nanotechnology has the ability of synthesising the metals in nanosize which drastically changes the chemical physical and optical properties of metals The aim of the present study was three fold i antibacterial efficacy of AgNps against E faecalis ii biocompatibility of AgNps by haemolytic assay iii effect of AgNps on the microhardness of root dentin In the present study AgNps was ...
Enterococcus is a large genus of lactic acid bacteria of the phylum Firmicutes. Enterococci are Gram-positive cocci that often occur in pairs (diplococci) or short chains, and are difficult to distinguish from streptococci on physical characteristics alone. Two species are common commensal organisms in the intestines of humans: E. faecalis (90-95%) and E. faecium (5-10%). Rare clusters of infections occur with other species, including E. casseliflavus, E. gallinarum, and E. raffinosus. Enterococci are facultative anaerobic organisms, i.e., they are capable of cellular respiration in both oxygen-rich and oxygen-poor environments. Though they are not capable of forming spores, enterococci are tolerant of a wide range of environmental conditions: extreme temperature (10-45 °C), pH (4.5-10.0), and high sodium chloride concentrations. Enterococci typically exhibit alpha-hemolysis on sheeps blood agar. Members of the genus Enterococcus (from Greek έντερο, éntero, intestine and κοκκος, ...
Many methods have been employed to prevent infections from opportunistic pathogens in immunocompromised individuals. Among these are the use of ultraviolet light (UV). In this study, UVC light, was found to have a deleterious effect on specific skin flora. Organisms tested included Acinetobacter baumanii, Candida albicans, Candida kefyr, Corynebacterium renale, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, and Streptococcus pyogenes. Nutrient agar was used to cultivate most organisms. Blood agar was used for the cultivation of Streptococcus pyogenes and Enterococcus faecalis. Bacterial suspensions were made and utilized to plate each organism onto a set of 7 nutrient agar or blood agar plates. A portion of each of 6 of the plates was then exposed to UVC light for 15, 30, 45, 60, 75, and 90 seconds respectively. For each organism, increased exposure to UVC light resulted in a decrease of the number of colony forming units observed in the portion of the
I have recently been in the situation of dealing with a number of blood cultures which have grown the bacteria Enterococcus sp. Whilst not a particularly exciting bacteria, its presence in blood cultures should always be taken seriously. One particular patient had Enterococcus faecalis in 3 sets of blood cultures and was being treated for pneumonia on the basis of a fever and a few crackles in his chest. I went to the ward to review the patient and although a junior later commented that you always come up with an amazing diagnosis! it was The Microbiology which led to the correct answer, but I took the compliment anyway ...
EPA does not allow any antimicrobial product to claim that it makes the home or other use site safe, and also does not allow natural claims (which they believe implies safety; we use the term inherent instead). In addition, EPA requires Antimicrobial Copper product advertising and labeling to indicate that while they have been shown to reduce microbial contamination, they do not necessarily prevent cross-contamination. Claims cannot be made regarding protection from the actual acquisition or transmission of infectious pathogens.. EPA also requires that advertising and labeling clearly indicate that the use of Antimicrobial Copper surfaces is a supplement to, but not a substitute for routine cleaning of the product.. Further, Antimicrobial Copper surfaces are only registered to make public health claims against the following five bacteria: Vancomycin-resistant Enterococcus faecalis(VRE), Methicillin-resistant Staphylococcus aureus, Enterobacter aerogenes, Pseudomonas aeruginosa, ...
Screening a biobank of over 600 environmental isolates referencing previous partial characterisation data resulted in the selection of eleven Enterococcus faecalis and four E. faecium strains that exhibited potential for conjugation via a pheromone-dependent pathway. These isolates had prolific and diverse antimicrobial resistance profiles. Conjugal transfer of antibiotic resistance phenotypes was determined using a solid agar mating method followed by a standard antibiotic selection test resulting in different transfer patterns. Multiple gene transfer was observed in single reactions. An interspecies conjugal transfer of vancomycin resistance from E. faecalis to E. faecium was identified while the remaining reactions were within the same species. Transfer efficiencies ranging from 2 × 10−1 to 2.3 × 10−5 were determined. Interspecies transfer of vancomycin resistance among environmental isolates of enterococci had not previously been characterised, along with alternating transfer of ...
Table of Contents:. Part I Variable vane pump Industry Overview. Chapter One Variable vane pump Industry Overview. 1.1 Variable vane pump Definition. 1.2 Variable vane pump Classification Analysis. 1.2.1 Variable vane pump Main Classification Analysis. 1.2.2 Variable vane pump Main Classification Share Analysis. 1.3 Variable vane pump Application Analysis. 1.3.1 Variable vane pump Main Application Analysis. 1.3.2 Variable vane pump Main Application Share Analysis. 1.4 Variable vane pump Industry Chain Structure Analysis. 1.5 Variable vane pump Industry Development Overview. 1.5.1 Variable vane pump Product History Development Overview. 1.5.1 Variable vane pump Product Market Development Overview. 1.6 Variable vane pump Global Market Comparison Analysis. 1.6.1 Variable vane pump Global Import Market Analysis. 1.6.2 Variable vane pump Global Export Market Analysis. 1.6.3 Variable vane pump Global Main Region Market Analysis. 1.6.4 Variable vane pump Global Market Comparison Analysis. 1.6.5 ...
SWISS-MODEL Repository entry for P21598 (TET9_ENTFL), Tetracycline resistance protein TetM from transposon Tn916. Enterococcus faecalis (Streptococcus faecalis)
Ceftriaxone is an antibiotic used to treat many kinds of bacterial infections, including severe or life-threatening forms such as meningitis, Acinetobacter and Enterobacter species, hemophilic influenzae (including beta lactamase producing strains), Klebsiellosis pneumoniae, organelle, Neisseria and Proteus species, and Serratia marcescens. It possesses activity against most strains of Staphylococcus aureus and Streptococcus pneumoniae, but Staphylococcus epidermidis, methicillin-resistant strains of staphylococcus, and Enterococcus faecalis (Group D streptococci) are typically resistant. Ceftriaxone has poor activity against anaerobes. Ceftriaxone has a long serum life, time that the drug takes for its concentration in the body to reduce by half compared to other antibiotics for bacterial infections. This allows it to be taken after twelve hours in children and twenty four hours in adults. However the use of Ceftriaxone causes leads to serious side effects that include severe skin reactions. ...
CLYRA™ technology causes the instantaneous release of a powerful yet safe antimicrobial in measured doses when it is mixed by water from any source, including a wound exudate caused to insult an impregnated bandage. CLYRA has characterized this antimicrobial in conformance with FDA standards, and confirms that the efficacy and safety of this antimicrobial is in conformance with standards and literature on the subject. CLYRA Medical Technologies continues to push the envelope with efficacy and safety testing.. Formulations of CLYRAs antimicrobial hydrogel and liquid wound cleanser products have passed rigorous third party laboratory testing validating both efficacy and safety. Independent testing has verified both rapid and effective control against a host of dangerous pathogens commonly encountered in the wound care field. Testing of the companys liquid formula verified efficacy against antimicrobial resistant species referred to by the CDC as the ESKAPE pathogens - enterococcus faecium ...
Highly virulent enterococcal strains have a pathogenicity island within their genome that encodes, among other traits, a cytolytic toxin that uses a quorum-sensing mechanism to affect autoinduction. Coburn et al. (see the Perspective by Garsin) show that the bacterium actively secretes two components, an autoinducer and an anti-autoinducer. In the absence of target cells, these two interact and prevent the autoinducer from feeding back to induce high-level expression of the cytolysin. In the presence of the target cell, however, the anti-autoinducer binds to the target cell and allows the autoinducer to accumulate to the threshold level required for quorum induction of the cytolysin operon. The anti-autoinducer is itself a toxin component and effectively tags the target for destruction. P. S. Coburn, C. M. Pillar, B. D. Jett, W. Haas, M. S. Gilmore, Enterococcus faecalis senses target cells and in response expresses cytolysin. Science 306, 2270-2272 (2004). [Abstract] [Full Text]. D. A. Garsin, ...
A synthetic bioredox system for the oxidative decarboxylation of L-malate to pyruvate was presented, with which the enzyme purification steps can be omitted. The bioredox system consisted of ME-t (mutant malic enzyme ME L310R/Q401C) and non-natural coenzyme nicotinamide flucytosine dinucleotide (NFCD+) catalyzes the oxidation of L-malate to pyruvate and concurrently generates the reduced coenzyme, NFCDH. Escherichia coli BL21(DE3) pET24b-ME-t cell lysates catalyze the oxidation of L-malate and produce pyruvate only, indicating that the cell lysates have the same substrate selectivity as the purified enzyme. NADH oxidase (NOX) from Enterococcus faecalis can oxidize NFCDH to regenerate NFCD+. Compared with the reaction system containing NAD(+), ME lysates, and NOX lysates, the system containing NFCD+, ME-t lysates, and NOX lysates converts L-malate to pyruvate in 9% higher yield and with significantly less lactate formation. These results suggest that the synthetic bioredox system composed of ME-t ...
Gram positive bacteria - Enterococcus What are Gram positive bacteria - enterococci? Enterococci are ubiquitous gram-positive cocci, calatase-negative, non-spore-forming, facultative anaerobic organisms, that belong to the Lancefield group D streptococci. Enterococci are normally present, as colonizers, in the intestinal tract of human beings and animals, and can be recovered from feces in large quantities. Enterococci…. ...
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Changes in antimicrobial resistance of Enterococcus spp. Over a few years., Yeliz Tanriverdi Cayci, Keramettin Yanik, Adil Karadag, Hava Yilmaz, Saban Esen, Murat Gunaydin
A clinically relevant porcine model of a biofilm-infected wound was established in 10 minipigs. The wounds of six experimental animals were infected with a modified polymicrobial Lubbock chronic wound biofilm consisting of Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Bacillus subtilis. Four animals served as uninfected controls. The wounds were monitored until they had healed for 24 days. The biofilm persisted in the wounds up to day 14 and significantly affected healing. The control to infected healed wound area ratios were: 45%/21%, 66%/37%, and 90%/57% on days 7, 10 and 14, respectively. The implanted biofilm prolonged inflammation, increased necrosis, delayed granulation and impaired development of the extracellular matrix as seen in histological and gene expression analyses. This model provides a therapeutic one-week window for testing of anti-biofilm treatments and for research on the pathogenesis of wound infections in pig that is clinically the most relevant ...
Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Putative pheromone cAM373 precursor lipoprotein CamS ...
Aureo for Pet is recommended on the following conditions: Sick Decrepitude Poor activity Poor Appetite Prevent diseases EF (Enterococcus Faecalis) lactic acid b
... and faecalis, or the enterococcus. Horder, as well as John Hannah Drysdale, Hugh Thursfield, Frank Atcherley Rose, and W. ...
Enterococcus faecalis) and their phage. The genes coding for the lytic system of the pneumococcal phage, Dp-1, has been cloned ... "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis". Science. 299 (5615): 2071-2074. Bibcode: ...
Kemp KD, Singh KV, Nallapareddy SR, Murray BE (November 2007). "Relative contributions of Enterococcus faecalis OG1RF sortase- ... a second pilus gene cluster in Enterococcus faecalis?". Current Microbiology. 59 (2): 206-11. doi:10.1007/s00284-009-9422-y. ... "Endocarditis and biofilm-associated pili of Enterococcus faecalis". The Journal of Clinical Investigation. 116 (10): 2799-807. ...
... faecalis, E. faecium, E. durans, and E. avium). For example, Streptococcus faecalis is now Enterococcus faecalis. E. faecalis ... Köhler W (June 2007). "The present state of species within the genera Streptococcus and Enterococcus". International Journal of ... Many former group D streptococci have been reclassified and placed in the genus Enterococcus (including E. ... many bacteria formerly grouped in the genus Streptococcus were separated out into the genera Enterococcus and Lactococcus. ...
Enterococcus faecalis did this by releasing a bacteriocin. Female hoopoes transfer preen oil onto their brood patches and eggs ... One study of Eurasian hoopoes showed that the presence of symbiotic bacteria (Enterococcus faecalis) in their preen oil ...
... aeruginosa Serratia marcescens Enterococcus faecalis Peptococcus spp. Peptostreptococcus spp. Mezlocillin can be made in a ...
... a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405". Antimicrobial Agents and Chemotherapy. 43 (9 ... "The vanG glycopeptide resistance operon from Enterococcus faecalis revisited". Molecular Microbiology. 50 (3): 931-48. doi: ... Dutka-Malen S, Molinas C, Arthur M, Courvalin P (March 1992). "Sequence of the vanC gene of Enterococcus gallinarum BM4174 ... in Enterococcus casseliflavus". Microbial Drug Resistance. 15 (1): 1-9. doi:10.1089/mdr.2009.0856. PMID 19216682. D-alanine-D- ...
... and Enterococcus faecalis. Hospital-acquired infections may be due to coliform bacteria and enterococci, as well as other ...
UNC41MFS5, Enterococcus faecalis, and Enterococcus faecalis 13-SD-W-01. In the C1orf131 orthologs the DUF domains are typically ...
Real-time PCR method to detect Enterococcus faecalis in water. Biotechnology Letters, 25, 3, 261-5. S J Corbett, G L Rubin, G K ... Enterococcus is a large genus of lactic acid phylum Firmicutes Fecal enterococci inhabit gastrointestinal tract of animals. ... detection of the faecal indicator bacterium Enterococcus faecalis in water stored in traditional vessels. The Indian Journal of ... Enterococcus bacteria is not harmful by itself, but it indicates harmful bacteria is in the water. These types of bacteria can ...
"The incongruent gelatinase genotype and phenotype in Enterococcus faecalis are due to shutting off the ability to respond to ... "Structure-activity relationship of gelatinase biosynthesis-activating pheromone of Enterococcus faecalis". J. Bacteriol. 191 (2 ... pheromone abbreviated as GBAP is a cyclic peptide produced by pathogenic bacteria such as Enterococcus faecalis. GAP is part of ...
Enterococcus faecalis and/or other facultative enteric bacteria or Pseudomonas sp. are found in this situation. Endodontic ...
Maadani A, Fox KA, Mylonakis E, Garsin DA (May 2007). "Enterococcus faecalis mutations affecting virulence in the ...
"Ethanolamine Activates a Sensor Histidine Kinase Regulating Its Utilization in Enterococcus faecalis". Journal of Bacteriology ... "Multiple posttranscriptional regulatory mechanisms partner to control ethanolamine utilization in Enterococcus faecalis". ...
"Modular design of the Enterococcus hirae muramidase-2 and Streptococcus faecalis autolysin". FEMS Microbiology Letters. 70 (3 ... The region was originally identified as a C-terminal repeat found in the Enterococcus hirae muramidase. The LysM domain is ...
Enterococcus faecalis, Enterococcus faecium, Enterococcus durans and Streptococcus bovis Group F, G & L - Streptococcus ... Priloska, G (2008). "Virulence factors and antibiotic resistance in Enterococcus faecalis isolated from urine samples". Prilozi ... Notably, Enterococci and Streptococcus bovis (Lancefield Group D) are not beta-hemolytic. Though there are many groups of ... Enterococcus, formerly known as Group D Streptococcus, were classified as members of the genus Streptococcus until 1984 and ...
Weaver KE, Jensen KD, Colwell A, Sriram SI (April 1996). "Functional analysis of the Enterococcus faecalis plasmid pAD1-encoded ... "An intramolecular upstream helix ensures the stability of a toxin-encoding RNA in Enterococcus faecalis". J. Bacteriol. 191 (5 ... "Isolation of a derivative of Escherichia coli-Enterococcus faecalis shuttle vector pAM401 temperature sensitive for maintenance ... determinant is a 400 bp locus of the pAD1 plasmid which encodes a type I toxin-antitoxin system in Enterococcus faecalis. It ...
Multidrug-resistant Enterococcus faecalis and Enterococcus faecium are associated with nosocomial infections. These strains ... include: penicillin-resistant Enterococcus, vancomycin-resistant Enterococcus, and linezolid-resistant Enterococcus. ...
Tsigrelis C, Singh KV, Coutinho TD, Murray BE, Baddour LM (February 2007). "Vancomycin-Resistant Enterococcus faecalis ... cell wall contains a thick layer of peptidoglycan and no outer membrane-notably Enterococcus faecium and Enterococcus faecalis ... Acquired resistance to linezolid was reported as early as 1999, in two patients with severe, multidrug-resistant Enterococcus ... Ang JY, Lua JL, Turner DR, Asmar BI (December 2003). "Vancomycin-resistant Enterococcus faecium endocarditis in a premature ...
2003). "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis". Science. 299 (5615): 2071-4. ...
... extracts have weak antibacterial activity against Enterococcus faecalis and Staphylococcus aureus. "Virola ...
... and Enterococcus faecalis have developed resistance to imipenem to varying degrees. Not many species are resistant to imipenem ... It is particularly important for its activity against Pseudomonas aeruginosa and the Enterococcus species. It is not active ...
Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and ... Staphylococcus, Enterococcus, Pseudomonas, Escherichia and Klebsiella, Salmonella Antimicrobial. S. mauritiana. Roots and ...
... also shows in vitro activity against vancomycin-susceptible Enterococcus faecium and Enterococcus faecalis. Other ... may show in vitro susceptibility, and dalbavancin may exhibit activity against enterococci expressing the VanB or VanC ...
... such as Enterococcus faecalis, Staphylococcus and Clostridium perfringens. A diverse range of studies has been done on ... "Complete genome sequence and comparative genomic analysis of Enterococcus faecalis EF-2001, a probiotic bacterium". Genomics. ...
One of the two DUF1646 RNAs in Enterococcus faecalis was independently detected by term-seq. Data from both discoveries suggest ... A DUF1646 RNA occurs upstream of an operon in Enterococcus hirae that was shown to regulate the downstream genes based on ... operon in Enterococcus hirae". Archives of Microbiology. 178 (3): 172-179. doi:10.1007/s00203-002-0440-4. PMID 12189418. S2CID ...
"An Intramolecular Upstream Helix Ensures the Stability of a Toxin-Encoding RNA in Enterococcus faecalis". Journal of ...
... and characterization of a novel endo-alpha-N-acetylgalactosaminidase from Enterococcus faecalis". Biochemical and Biophysical ...
... vancomycin-susceptible Enterococcus faecalis, methicillin-resistant Staphylococcus aureus (MRSA), Streptococcus anginosus grp ... vancomycin-susceptible Enterococcus faecalis, methicillin-resistant Staphylococcus aureus (MRSA), Streptococcus agalactiae, ...
Enterococcus faecalis, Vibrio sp., and Lactobacillus reuteri. Bacteria which give negative results for the indole test include ...
Enterococcus. *BEA+: Enterococcus faecalis *Urinary tract infection. *Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
Enterococcus. *BEA+: Enterococcus faecalis *Urinary tract infection. *Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
"Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb ... and Enterococcus faecium comb. nov.". Int. J. Sys. Bacteriol. 34: 31-34. doi:10.1099/00207713-34-1-31.. ... Enterococcus é un xénero de bacterias do ácido láctico do filo Firmicutes. Os enterococos son cocos grampositivos, que adoitan ... 2002). The Enterococci: Pathogenesis, Molecular Biology, and Antibiotic Resistance. Washington, D.C.: ASM Press. ISBN 978-1- ...
The presence of Gram positive bacteria such as Enterococcus and Staphylococcus increased.[41] ... and Enterococcus (7%) among others.[6][24][25] Urinary tract infections due to Staphylococcus aureus typically occur secondary ...
... has been a leading cause of multi-drug resistant enterococcal infections over Enterococcus faecalis in the ... Enterococcus faecium is a Gram-positive, alpha-hemolytic or nonhemolytic bacterium in the genus Enterococcus.[1] It can be ... "In Gilmore MS, Clewell DB, Ike Y. Enterococci: From Commensals to Leading Causes of Drug Resistant Infection. PMID 24649504.. ... Enterococci: From Commensals to Leading Causes of Drug Resistant Infection. Boston: Massachusetts Eye and Ear Infirmary. PMID ...
Enterococcus faecalis bacterium dairy cream Enterococcus faecalis bacterium meat ham [2] Enterococcus faecalis bacterium soy ... Enterococcus faecalis bacterium vegetable pickle [2] Enterococcus faecalis bacterium meat sausage Enterococcus faecalis ... Enterococcus faecalis bacterium cheese [2] ...
The DNA sequence of the VRSA vanA gene was identical to that of a vancomycin-resistant strain of Enterococcus faecalis ... In vitro and in vivo experiments reported in 1992 demonstrated that vancomycin resistance genes from Enterococcus faecalis ... Strains of hVISA and VISA do not have resistant genes found in Enterococcus and the proposed mechanisms of resistance include ... Vancomycin MICs of the VRSA isolate were consistent with the VanA phenotype of Enterococcus species, and the presence of the ...
L. acidophilusen andui batzuek Enterococcus faecalis, Salmonella eta Staphylococcus aureus bakterio kaltegarrien hazkuntza, ...
Enterococcus. *BEA+: Enterococcus faecalis *Urinary tract infection. *Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
Enterococcus faecalis,[12] a Gram-positive bacterium. *Zymomonas mobilis,[citation needed] a Gram-negative facultative anaerobe ... Gram-positive bacteria such as Enterococcus faecalis,[7][full citation needed][page needed][better source needed] as well as ... "2-Ketogluconate fermentation by Streptococcus faecalis". J. Bacteriol. 87 (4): 844-851. doi:10.1128/JB.87.4.844-851.1964. PMC ...
... faecalis, E. faecium, E. durans, and E. avium).[14] For example, Streptococcus faecalis is now Enterococcus faecalis. E. ... Group D (enterococci)Edit. Many former group D streptococci have been reclassified and placed in the genus Enterococcus ( ... In 1984, many bacteria formerly considered Streptococcus were separated out into the genera Enterococcus and Lactococcus.[4] ... Köhler W (June 2007). "The present state of species within the genera Streptococcus and Enterococcus". International Journal of ...
"Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb ... and Enterococcus faecium comb. nov.". Int. J. Sys. Bacteriol. 34: 31-34. doi:10.1099/00207713-34-1-31. ... Enterococci are Gram-positive cocci that often occur in pairs or short chains. They look like streptococci.[1] Two species are ... 2002). The Enterococci: pathogenesis, molecular biology, and antibiotic resistance. Washington, D.C.: ASM Press. ISBN 978-1- ...
"Effects of single and combined supplementation of Enterococcus faecalis, mannan oligosaccharide and polyhydroxybutyrate acid on ...
... how treatment of MRSA infections has selected for vancomycin-resistant Enterococcus faecium, and the importance of antibiotic ... "SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and enterococcus ...
O Enterococcus faecalis e Enterococcus faecium resistentes a multidrogas están asociados a infeccións nosocomiais ( ... hospitalarias).[136] Entre estas cepas están: Enterococcus resistente á penicilina, Enterococcus resistente á vancomicina, e ... Streptococcus e Enterococcus[editar , editar a fonte]. As infeccións por Streptococcus pyogenes (Streptococcus do grupo A: GAS ... Enterococcus resistente á vancomicina, Acinetobacter baumannii resistente a multifármacos.[15] ...
Enterococcus. *BEA+: Enterococcus faecalis *Urinary tract infection. *Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
One study has shown that Enterococcus faecalis was still present in the dentin after 40 minutes of exposure of 1.3% and 2.5% ... sodium hypochlorite, whereas 40 minutes at a concentration of 5.25% was effective in E. faecalis removal.[48] In addition to ...
Enterococcus faecalis, Enterobacter cloacae, Staphylococcus epidermidis, and Klebsiella pneumoniae.[42]. Other usesEdit. ...
Enterococcus faecalis တို့ကြောင့်ဖြစ်သည်။ ဆေးရုံတွင်ဖြစ်သည့် ကျောက်ကပ်ရောင်ရောဂါများမှာ coliform နှင့် enterococci ဘက် ...
Salmonella enterica or Enterococcus faecalis.[46]. Invertebrates such as millipedes, insects, isopods, and gastropods can ...
Three main resistance variants have been characterised to date among resistant Enterococcus faecium and E. faecalis populations ... Peter J Collignon (1999). "Vancomycin-resistant enterococci and use of avoparcin in animal feed: is there a link?". Med J Aust ... In enterococci, this modification appears to be due to the expression of an enzyme that alters the terminal residue. ... Vancomycin-resistant Enterococcus emerged in 1987. Vancomycin resistance evolved in more common pathogenic organisms during the ...
Enterococcus. *BEA+: Enterococcus faecalis *Urinary tract infection. *Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
... and Enterococcus faecalis) than newer fluoroquinolones.[36]. Bacterial resistance[edit]. See also: Antibiotic abuse and ... Numerous pathogens, including enterococci, Streptococcus pyogenes and Klebsiella pneumoniae (quinolone-resistant) now exhibit ... Clostridium innocuum and Enterococcus faecium strains have developed resistance to ciprofloxacin to varying degrees.[41] ...
... and Enterococcus faecalis.[3][4] Tedizolid is a second-generation oxazolidinone derivative that is 4-to-16-fold more potent ... against staphylococci and enterococci compared to linezolid.[5] The recommended dosage for treatment is 200 mg once daily for a ...
Enterococcus faecalis NCTC12201, and E. faecalis NCTC12203 (both VRE). Bottromycin A2 had low micromolar activity against all ... It has been shown to inhibit methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) ... Antibiotic Streptomyces Secondary metabolite Peptide MRSA Vancomycin-resistant Enterococcus Waisvisz, J. M. (1957). " ... a potent antibiotic active against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococci". Chem. ...
Enterococcus. *BEA+: Enterococcus faecalis *Urinary tract infection. *Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
Europäische Faulbrut (EFB) - Haupterreger: Melissococcus plutonius, daneben: Enterococcus faecalis, Paenibacillus alvei, ...
Enterococcus. *BEA+: Enterococcus faecalis *Urinary tract infection. *Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
Enterococcus. BEA+: Enterococcus faecalis (Urinary tract infection) · Enterococcus faecium. Bacillales. (Cat+). Staphylococcus ...
Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis, and Streptococcus species.[5] Medical ...
Wikimedia Commons has media related to Enterococcus faecalis.. *Type strain of Enterococcus faecalis at BacDive - the Bacterial ... Prior to 1984, enterococci were members of the genus Streptococcus; thus, E. faecalis was known as Streptococcus faecalis.[24] ... "Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus nom. rev. as Enterococcus faecalis comb ... Enterococcus faecalis - formerly classified as part of the group D Streptococcus system - is a Gram-positive, commensal ...
Enterococcus faecalis (Streptococcus faecalis)Imported. Automatic assertion inferred from database entriesi ... tr,Q9AL06,Q9AL06_ENTFL Uncharacterized protein OS=Enterococcus faecalis OX=1351 PE=4 SV=1 ...
Most Washoe County offices will be closed Monday, February 18, in observance of Presidents Day, including Administrative offices, Social Services, Libraries, Courts, Attorneys Office, Washoe County Sheriffs Office, Regional Animal Services and Health District. The County Clerk at the Washoe County Complex on Ninth St. and Wells Ave., in Reno will remain open throughout the holiday from 8 a.m. to midnight for the issuance of marriage licenses, fictitious firm names and notary bond filings, as well as property tax payments for the Treasurers Office ...
Enterococcus faecalis X98 genomic scaffold supercont1.1, whole genome shotgun sequence. GenBank: GG688423.1 ...
Enterococcus faecalis is the most commonly isolated of the genera. According to Elmer Koneman, M.D., it is associated with 80 ... Enterococcus faecalis and all of the enterococci are closely related to the streptococci and appear microscopically as spheres ... One of the most important and serious factors about Enterococcus faecalis and the other enterococci is its resistance to a ... "Enterococcus faecalis,infection with enterococci,vancomycin resistance]"} Get the latest tips on diet, exercise and ...
Enterococcus faecalis. Mutation(s): 6 Gene Names: lacC. EC: 2.7.1.144. Find proteins for Q833W9 (Enterococcus faecalis (strain ... Crystal structure of LacC from Enterococcus faecalis. Gorman, J., Shapiro, L.. To be published. ...
Enterococcus faecalis (Streptococcus faecalis)Imported. Automatic assertion inferred from database entriesi ... tr,Q8KMU4,Q8KMU4_ENTFL EJ97 enterocin OS=Enterococcus faecalis OX=1351 GN=ej97a PE=4 SV=1 ...
Enterococcus faecalis, formerly known as Streptococcus faecalis, is a gram-positive bacterium with a low G+C content in its ... Enterococcus faecalis cells cannot synthesize porphyrins and do not rely on heme for growth but can take up heme and use it to ... Enterococcus faecalis Heme-Dependent Catalase Message Subject (Your Name) has forwarded a page to you from Journal of ... Enterococcus faecalis V583 contains a cytochrome bd-type respiratory oxidase. J. Bacteriol.182:3863-3866. ...
2007) Enterococcus faecalis Mutations Affecting Virulence in the C. elegans Model Host. Infect Immun 75:2634-2637. ... 2000) Enterococcus faecalis adhesin, ace, mediates attachment to extracellular matrix proteins collagen type IV and laminin as ... 2003) Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science 299:2071-2074. ... 2008) Ethanolamine activates a sensor histidine kinase regulating its utilization in Enterococcus faecalis. J Bacteriol 12:7147 ...
A family of fibrinogen-binding MSCRAMMs from Enterococcus faecalis.. Sillanpää J1, Nallapareddy SR, Houston J, Ganesh VK, ... We report that three (EF0089, EF2505 and EF1896, renamed here Fss1, Fss2 and Fss3, respectively, for Enterococcus faecalis ... Effect of insertional inactivation of fss2 on surface expression of Fss2 and adherence of E. faecalis OG1RF to Fg. (a) Flow- ... Binding of the three E. faecalis MSCRAMMs to human Fg. (a) A collection of recombinant segments from Fss1, Fss2 and Fss3 were ...
Enterococcus faecalis ATCC ® 19433NA™ Designation: NCTC 775 TypeStrain=True Application: Control Culture Media testing Quality ...
Enterococcus faecalis ATCC ® 51299™ Designation: NJ-3 TypeStrain=False Application: Quality control strain Susceptibility disc ... Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299™) Strain Designations: NJ-3 / Type Strain: ... Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC® 51299-MINI-PACK™) Add to ... Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz ATCC® 51299™ freeze-dried ...
Enterococcus faecalis. Mutation(s): 0 Gene Names: mvaS. EC: 4.1.3.5 (PDB Primary Data), 2.3.3.10 (UniProt). ... X-ray Crystal Structures of HMG-CoA Synthase from Enterococcus faecalis and a Complex with Its Second Substrate/Inhibitor ... Crystal structure of HMG-CoA synthase from Enterococcus faecalis. *DOI: 10.2210/pdb1X9E/pdb ... the structure of HMG-CoA synthase from Enterococcus faecalis (MVAS) was determined by selenomethionine MAD phasing to 2.4 A and ...
Esp-Independent Biofilm Formation by Enterococcus faecalis. Christopher J. Kristich, Yung-Hua Li, Dennis G. Cvitkovitch, Gary M ... Esp-Independent Biofilm Formation by Enterococcus faecalis. Christopher J. Kristich, Yung-Hua Li, Dennis G. Cvitkovitch, Gary M ... Esp-Independent Biofilm Formation by Enterococcus faecalis. Christopher J. Kristich, Yung-Hua Li, Dennis G. Cvitkovitch, Gary M ... Esp-Independent Biofilm Formation by Enterococcus faecalis Message Subject (Your Name) has forwarded a page to you from Journal ...
Gene context of Enterococcus faecalis. *The flavoprotein NADH oxidase from Streptococcus faecalis 10C1, which catalyzes the ... The pathogenesis of Enterococcus (Streptococcus) faecalis was studied in mice with E. faecalis intestinal overgrowth (10(9) - ... Evidence for the translocation of Enterococcus faecalis across the mouse intestinal tract. Wells, C.L., Jechorek, R.P., ... Endocarditis due to streptomycin-susceptible Enterococcus faecalis with high-level gentamicin resistance. Spiegel, C.A., Huycke ...
We show that the tetrameric small alarmone synthetase (SAS) RelQ from the Gram-positive pathogen Enterococcus faecalis is a ... 2009) The molecular alarmone (p)ppGpp mediates stress responses, vancomycin tolerance, and virulence in Enterococcus faecalis. ... mRNA is a potent inhibitor of ppGpp synthesis by E. faecalis RelQ. (A) 3H ppGpp synthesis activity of 250 nM E. faecalis RelQ ( ... Negative allosteric regulation of Enterococcus faecalis small alarmone synthetase RelQ by single-stranded RNA. Jelena ...
Enterococcus faecalis,/i, and ,i,Enterococcus faecium,/i,. For this purpose, 79 clinical isolates of Malaysian enterococci were ... In conclusion, the pathogenicity of ,i,E. faecalis,/i, and ,i,E. faecium,/i, could be associated with different virulence ... i,Enterococcus,/i,, a Gram-positive facultative anaerobic cocci belonging to the lactic acid bacteria of the phylum Firmicutes ... Among them only two, that is, Enterococcus faecium and Enterococcus faecalis, are now being increasingly recognized to be ...
Pentose phosphate pathway - Enterococcus faecalis Symbioflor 1 [ Pathway menu , Organism menu , Pathway entry , Download KGML ...
... Masato Nagayoshi ... "Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model," ISRN Dentistry ...
Enterococcus faecalis appears in many tooth root infections and is not eliminated by root canal therapy. It can reside in tooth ... Nonetheless, E faecalis may be a "keystone" player in dental implant bone loss or peri-implantitis. That is, E faecalis may be ... However, E faecalis is not detectable with some culture techniques and thus can be missed. It is usually not a dominant species ...
From: Enterococcus faecalis YM0831 suppresses sucrose-induced hyperglycemia in a silkworm model and in humans ...
The complete genome sequence of Enterococcus faecalisV583, a vancomycin-resistant clinical isolate, revealed that more than a ... Role of Mobile DNA in the Evolution of Vancomycin-Resistant Enterococcus faecalis ... Role of Mobile DNA in the Evolution of Vancomycin-Resistant Enterococcus faecalis ... Role of Mobile DNA in the Evolution of Vancomycin-Resistant Enterococcus faecalis ...
Coloured scanning electron micrograph (SEM) of a clump of Enterococcus faecalis bacteria. This Gram-positive coccus (round ... Caption: Enterococcus faecalis bacteria. Coloured scanning electron micrograph (SEM) of a clump of Enterococcus faecalis ... enterococcus faecalis, false-colored, false-coloured, gram-positive, hai, hospital acquired, hospital-acquired infections, ...
... of Enterococcus faecalis and 25.2% of Enterococcus faecium isolates. Only 3.3% and 2.1% of E. faecalis isolates had elevated ... Enterococcus faecalis, and Enterococcus faecium clinical isolates collected from the China Antimicrobial Surveillance... ... Transferable Resistance Gene optrA in Enterococcus faecalis from Swine in Brazil OptrA is an ATP-binding cassette (ABC)-F ... Here, we isolated 13 Enterococcus faecalis strains possessing a linezolid MIC of ≥4 mg/liter from nursery pigs in swine herds ...
... and Vancomycin Resistant Enterococcus faecalis. or faecium (VRE) ... and/or Vancomycin Resistant Enterococcus faecalis or faecium ( ... and/or Vancomycin Resistant Enterococcus faecalis or faecium (VRE) (pdf) ...
Enterococcus Faecalis*Escherichia Coli*Hydrocortisone*Pseudomonas Aeruginosa*Staphylococcus Aureus Cell Products. Available ...
Among enterococci, Enterococcus faecalis occurs ubiquitously, with the highest incidence of human and animal infections. The ... A Core Genome Multilocus Sequence Typing Scheme for Enterococcus faecalis. Bernd Neumann, Karola Prior, Jennifer K. Bender, Dag ... A Core Genome Multilocus Sequence Typing Scheme for Enterococcus faecalis. Bernd Neumann, Karola Prior, Jennifer K. Bender, Dag ... A Core Genome Multilocus Sequence Typing Scheme for Enterococcus faecalis. Bernd Neumann, Karola Prior, Jennifer K. Bender, Dag ...
Among enterococci, Enterococcus faecalis occurs ubiquitously, with the highest incidence of human and animal infections. The ... Comparative genomics of enterococci: variation in Enterococcus faecalis, clade structure in E. faecium, and defining ... Enterococcus faecalis urinary-tract infections: do they have a zoonotic origin? J Infect 73:305-313. doi:10.1016/j.jinf.2016.07 ... Biodiversity of Enterococcus faecalis based on genomic typing. Int J Food Microbiol 165:27-34. doi:10.1016/j.ijfoodmicro. ...
Endocarditis Enterococcus faecalis Bacteremia Echocardiography Management score Electronic supplementary material. The online ... faecalis compared to other enterococci [3, 4, 6, 7], prosthetic heart valves [3, 5, 7], heart murmur [2, 4], previous ... Risk factors of endocarditis in patients with Enterococcus faecalis bacteremia: external validation of the NOVA score. Clin ... The DENOVA score efficiently identifies patients with monomicrobial Enterococcus faecalis bacteremia where echocardiography is ...
  • For this purpose, 79 clinical isolates of Malaysian enterococci were evaluated for the presence of virulence genes. (hindawi.com)
  • The in vitro activities of ceftaroline and tedizolid were compared against Staphylococcus aureus , Enterococcus faecalis , and Enterococcus faecium clinical isolates collected from the China Antimicrobial Surveillance. (asm.org)
  • High-level aminoglycoside resistance was noted in 30.0% of Enterococcus faecalis and 25.2% of Enterococcus faecium isolates. (asm.org)
  • Only 3.3% and 2.1% of E. faecalis isolates had elevated daptomycin MIC (≥2 mg/liter) and vancomycin. (asm.org)
  • This study aimed to evaluate the in vitro antimicrobial activity, heteroresistance emergence, and resistance mechanism of omadacycline (OMC) in clinical Enterococcus faecalis isolates from China. (asm.org)
  • To test the E. faecalis cgMLST scheme and assess the typing performance, different collections comprising environmental and bacteremia isolates, as well as all publicly available genome sequences from the NCBI and SRA databases, were analyzed. (asm.org)
  • Clinical isolates of Enterococcus faecalis aggregate human platelets. (lu.se)
  • Here we report for the first time that many clinical isolates of Enterococcus faecalis, a common pathogen in infective endocarditis, aggregate human platelets. (lu.se)
  • Multiresistance (to five or more antimicrobials) was more common in E. faecalis isolates from BC (77.4% of isolates) samples than those from PC (25%) and RP (37.6%) samples. (pigprogress.net)
  • We evaluated the epidemiological and clinical characteristics of patients with bacteremias caused by HLGR and non_HLGR Enterococcus faecalis isolates at a teaching hospital in the State of São Paulo, Brazil. (scielo.br)
  • We identified 145 cases of E. faecalis bacteremia: 66 (45.5%) were caused by HLGR isolates and 79 (54.5%) by non_HLGR. (scielo.br)
  • E. faecalis bacteremia is associated with high mortality and is frequently caused by HLGR isolates at this teaching hospital. (scielo.br)
  • The variability among genotyped isolates suggests that endogenous infections, rather than patient-to-patient transmission of E. faecalis , are more common at this institution. (scielo.br)
  • This study aimed to determine the prevalence and characteristics of E. faecalis biofilm formation in a sample of clinical isolates and to explore the virulence factors associated with biofilm formation in those isolates. (frontiersin.org)
  • A total of 265 E. faecalis isolates were collected from patients in Shenzhen, China. (frontiersin.org)
  • The expression levels of the clinical E. faecalis isolates' genes were determined by quantitative real-time polymerase chain reaction. (frontiersin.org)
  • In conclusion, the ST16 classification and linezolid resistance were positively associated with strong/medium biofilm formation in clinical E. faecalis isolates. (frontiersin.org)
  • cylA was associated with weak biofilm formation, and esp was only associated with strong or medium biofilm formation of the clinical E. faecalis isolates. (frontiersin.org)
  • The most prevalent species cultured from humans, Enterococcus faecalis and Enterococcus faecium , account for more than 90% of clinical enterococcal isolates ( Fisher and Phillips, 2009 ). (frontiersin.org)
  • During the 20-month study period, 124 of 1506 isolates (8.2%) of E. faecalis from 70 patients were found to produce beta-lactamase. (annals.org)
  • Restriction enzyme digests of total chromosomal DNA showed nearly identical patterns for selected isolates of beta-lactamase-producing, high-level gentamicin-resistant E. faecalis , suggesting dissemination through the hospital of a single strain of E. faecalis . (annals.org)
  • In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen. (asm.org)
  • Characterization of Enterococcus faecalis isolates by Chicken Embryo Lethality Assay and ERIC-PCR. (upm.es)
  • Molecular, genomic and phenotypic analysis of representative isolates of a major clone of E. faecalis MLST ST40 revealed new insights into the microbiology of a commensal bacterium which can turn into a conditional pathogen. (biomedcentral.com)
  • Isolates were confirmed to be E. faecalis by polymerase chain reaction (PCR) and further by multilocus sequence typing (MLST) to state their population structure and comparison made to sequence types previously obtained from chicken. (dtu.dk)
  • A total of 101 isolates were confirmed as E. faecalis by a species specific PCR. (dtu.dk)
  • In this study, we investigated the prevalence of HLGR enterococci and their genetic relatedness in blood culture isolates from patients with bacteraemia admitted to the 3 hospitals in Östergötland, a county in the south east of Sweden, during 1994-2001. (diva-portal.org)
  • 36 of 250 E. faecalis (14%) and 4 of 106 E. faecium isolates (4%) were shown by PCR to carry the aac(6′)-Ie-aph(2″)-Ia aminoglycoside modifying gene and these isolates were also classified as HLGR enterococci by the gentamicin antibiotic disk diffusion method. (diva-portal.org)
  • A majority of HLGR E. faecalis isolates (83%) belonged to the same cluster of genetically related isolates, according to the pulsed-field gel electrophoresis (PFGE) patterns, whereas all 4 HLGR E. faecium isolates had unique PFGE patterns. (diva-portal.org)
  • Among clinical enterococcal isolates from patients admitted to Swedish ICUs, although vancomycin resistant enterococci (VRE) were only sporadically found, multidrug resistance was common. (diva-portal.org)
  • In this study we aimed to determine occurrence of esp in E. faecium and E. faecalis isolates isolated from urinary tract infections and to investigate whether there is any correlation between presence of esp and antibiotic resistance. (eurekamag.com)
  • 43.3% of isolates identified as E. faecium and 56.7% as E. faecalis. (eurekamag.com)
  • The esp gene was found in 76.1% of E. faecium isolates and 77.9% of E. faecalis isolate. (eurekamag.com)
  • The greatest number of E. faecalis with antibiotic resistance and virulence factors (i.e., gelatinase, cytolysin, enterococcus surface protein, and aggregation substance) originated from WWTF-1 that processed meat waste from a nearby commercial meat-processing plant, suggesting an agricultural rather than human clinical source of these isolates. (k-state.edu)
  • Characterization of glycopeptides, aminoglycosides and macrolide resistance among Enterococcus faecalis and Enterococcus faecium isolates from hospitals in Tehran. (semanticscholar.org)
  • Drug resistance of Enterococcus faecium clinical isolates and the conjugative transfer of gentamicin and erythromycin resistance traits. (semanticscholar.org)
  • From a total of 46 E. faecalis investigated in this study, VanB gene was present in 10.9% of the isolates while vanA gene was not detected at all. (alliedacademies.org)
  • The E. faecalis isolates were first sequence typed by multilocus sequence typing (MLST) and characterized with respect to antibiotic resistance and properties associated with virulence. (biomedcentral.com)
  • However, a higher incidence of some of the virulence determinants in clinical isolates may indicate that these genes enhance the ability of E. faecalis to cause disease, as suggested by virulence studies on bacterial mutants in animal models [ 3 ]. (biomedcentral.com)
  • In an attempt to study genetic variability of commensal E. faecalis isolates, we used genome-wide DNA arrays to probe the presence of 3219 open reading frames (ORFs) from V583 and 10 ORFs representing a 17-kb deletion in the V583 pathogenicity island in our collection of community-derived Norwegian fecal E. faecalis baby isolates. (biomedcentral.com)
  • However, recent reports have shown that the Enterococcus species are the most common organisms in isolates of postoperative endophthalmitis [ 2 , 3 ]. (ekjo.org)
  • Pulsed-field gel electrophoresis applied to isolates from the first and second infections revealed that the E. faecalis were of the same strain ( Fig. 1F ). (ekjo.org)
  • The enterococcal surface protein gene, esp , is a major putative pathogenicity marker in clinical isolates of Enterococcus faecium and Enterococcus faecalis . (oup.com)
  • Of the transconjugants analysed by PCR for acquisition of esp , only isolates from two E. faecalis and a single E. faecium mating were positive. (oup.com)
  • According to one study combined drug therapy has shown some efficacy in cases of severe infections (e.g. heart valves infections ) against susceptible strains of E. faecalis . (wikipedia.org)
  • Ampicillin - and vancomycin -sensitive E. faecalis (lacking high-level resistance to aminoglycosides ) strains can be treated by gentamicin and ampicillin antibiotics. (wikipedia.org)
  • Here, we isolated 13 Enterococcus faecalis strains possessing a linezolid MIC of ≥4 mg/liter from nursery pigs in swine herds located across Brazil. (asm.org)
  • By sequencing a representative collection of 146 E. faecalis strains isolated from hospital outbreaks, food, animals, and colonization of healthy human individuals, we established a novel cgMLST scheme with 1,972 gene targets within the Ridom SeqSphere + software. (asm.org)
  • We demonstrate in rats that leaking anastomotic tissues were colonized by E. faecalis strains that showed an increased collagen-degrading activity and also an increased ability to activate host MMP9, both of which contributed to anastomotic leakage. (sciencemag.org)
  • Either elimination of E. faecalis strains through direct topical antibiotics applied to rat intestinal tissues or pharmacological suppression of intestinal MMP9 activation prevented anastomotic leak in rats. (sciencemag.org)
  • These data suggest that carcasses entering the final product processing area are mostly free of E. faecalis but are recontaminated with antimicrobial-resistant strains during processing. (pigprogress.net)
  • and subsequent studies showed that the hemolysin/bacteriocin trait exhibited by many E. faecalis strains usually involved a conjugative plasmid (4, 6). (springer.com)
  • To determine the presence and expression of Enterococcus faecalis Esp gene in several strains from an in vitro model on extracted teeth. (isciii.es)
  • Enterococcus faecalis are a major cause of nosocomial infection worldwide, and the spread of vancomycin resistant strains (VRE) limits treatment options. (diva-portal.org)
  • The polysaccharide antigen was not detectable in E. faecalis strains, however. (asm.org)
  • Enterococcus faecalis strains are resident intestinal bacteria associated with invasive infections, inflammatory bowel diseases, and colon cancer. (asm.org)
  • We generated E. faecalis strains containing deletions/point mutations in this PTS and measured bacterial growth and PTS gene expression in minimal medium supplemented with selected carbohydrates. (asm.org)
  • We show that E. faecalis upregulates OG1RF_12399 transcription specifically in the presence of gluconate and that E. faecalis strains lacking, or harboring a single point mutation in, OG1RF_12399-12402 are unable to grow in minimal medium containing gluconate. (asm.org)
  • We colonized germfree wild-type and colitis-prone interleukin-10-deficient mice with defined bacterial consortia containing the E. faecalis strains and measured inflammation and bacterial abundance in the colon. (asm.org)
  • We infected macrophage and intestinal epithelial cell lines with the E. faecalis strains and measured intracellular bacterial survival and proinflammatory cytokine secretion. (asm.org)
  • Given the difficulty of estimating the risk from field strains, the Embryo Lethality Assay (ELA) is proposed in this study as model to predict the virulence of 68 avian E. faecalis strains. (upm.es)
  • Additionally, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) was used to characterize the genetic diversity of the E. faecalis strains. (upm.es)
  • The ELA was performed ten times with subsets of seven-eight E. faecalis strains each on a sample of 9987 eggs including control groups. (upm.es)
  • The ELA was able to distinguish the virulence of the E. faecalis strains. (upm.es)
  • In conclusion, the ELA can be considered a reliable and useful tool to predict the virulence of avian E. faecalis strains. (upm.es)
  • We generated the first circular closed reference genome of an E. faecalis isolate D32 of animal origin and compared it with the genomes of other reference strains. (biomedcentral.com)
  • Bacteraemia with HLGR enterococci in Östergötland was mainly due to the spread of a cluster related of E. faecalis strains. (diva-portal.org)
  • Finally, we show in humans undergoing colon surgery and treated with the standard recommended intravenous antibiotics that their anastomotic tissues still contained E. faecalis and other bacterial strains with collagen-degrading/MMP9-activating activity. (igsb.org)
  • The variations in gene content observed among the investigated commensal E. faecalis is comparable to the genetic variation previously reported among strains of various origins thought to be representative of the major E. faecalis lineages. (biomedcentral.com)
  • However, an effort to explore the gene content of commensal E. faecalis by CGH has not previously been made and little is known about genetic determinants that may explain the differences in life style between pathogenic and non-pathogenic E. faecalis strains. (biomedcentral.com)
  • Preinduction of E. faecalis donors with s.g.cAM373 resulted in transconjugation frequencies in non-pheromone producing strains of Streptococcus mutans , Streptococcus sanguinis , Streptococcus anginosus , and Streptococcus suis that were significantly higher than frequencies when donors were preincubated with s.g.cAM373-R or medium alone. (microbiologyresearch.org)
  • Nine E. faecalis strains were tested: six obtained from patients presenting endocarditis, one with isolated bacteremia, and two reference strains. (pasteur.fr)
  • In the present work we have used proteomic and microarray expression profiling of E. faecalis JH2-2 and an ers-deleted mutant (Δ ers mutant) strains to define the Ers regulon. (archives-ouvertes.fr)
  • As such, enterococci, mainly vancomycin-resistant strains, are an appropriate target for phage therapy ( 4 ). (asm.org)
  • Our data contain electrophoresis of E. faecalis DNA cultured from two cases, which showed that the two strains isolated from the cases were exactly identical. (ekjo.org)
  • In the last two decades, particularly virulent strains of Enterococcus that are resistant to vancomycin (vancomycin-resistant Enterococcus, or VRE) have emerged in nosocomial infections of hospitalized patients, especially in the US. (wikipedia.org)
  • abstract = "Enterococcus faecalis cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. (dtu.dk)
  • Enterococcus faecalis - formerly classified as part of the group D Streptococcus system - is a Gram-positive , commensal bacterium inhabiting the gastrointestinal tracts of humans. (wikipedia.org)
  • Enterococcus faecalis , formerly known as Streptococcus faecalis , is a gram-positive bacterium with a low G+C content in its genomic DNA. (asm.org)
  • Correspondingly, several bacterial species found within the GI tract encode the machinery necessary for ethanolamine catabolism, including, but not limited to, E. faecalis , Streptococcus sanguinis , Escherichia coli , and Salmonella , Listeria , and Clostridium species. (pnas.org)
  • In an effort to determine the relevance of using Western blot (WB) for the etiological diagnosis of BCNE in patients with early antibiotic use, we developed specific assays for the major infective endocarditis (IE) causative agents, namely, Staphylococcus aureus, Enterococcus faecalis, Streptococcus anginosus , and Streptococcus gallolyticus . (frontiersin.org)
  • They identified an R-plasmid (pJH1) and a hemolysin/ bacteriocin plasmid (pJH2) in a clinical isolate of Enterococcus (formerly Streptococcus ) faecalis and found both capable of transfer in broth matings (38, 39). (springer.com)
  • Christie, P.J., and Dunny, G.M., 1986, Identification of regions of the Streptococcus faecalis plasmid pCF-10 that encode antibiotic resistance and pheromone response functions, Plasmid 15: 230-241. (springer.com)
  • Hirt H, Erlandsen SL, Dunny GM (2000) Heterologous inducible expression of Enterococcus faecalis pCF10 aggregation substance Asc10 in Lactococcus lactis and Streptococcus gordonii contributes to cell hydrophobicity and adhesion to fibrin. (springer.com)
  • To improve drug screening of species-selective antimicrobials, Cytoskeleton offers FtsZ proteins from E. coli, Staphylococcus aureus, Streptococcus pneumoniae and Enterococcus faecalis . (cytoskeleton.com)
  • Streptococcus gordonii produces a pheromone heptapeptide, s.g.cAM373, which induces a conjugative mating response in Enterococcus faecalis cells carrying the responsive plasmid, pAM373. (microbiologyresearch.org)
  • Schleifer KH, Kilpper-Bälz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. (dsmz.de)
  • Members of the genus Enterococcus (from Greek έντερο, éntero, "intestine" and κοκκος, coccos, "granule") were classified as group D Streptococcus until 1984, when genomic DNA analysis indicated a separate genus classification would be appropriate. (wikipedia.org)
  • We recently described a cytochrome bd in E. faecalis strain V583 and here report the identification of a chromosomal gene, katA , encoding a heme-containing cytoplasmic catalase. (asm.org)
  • Recognizing the medical importance of this bacterium, The Institute for Genomic Research has undertaken the genome sequencing of E. faecalis strain V583. (asm.org)
  • E. faecalis cells do not synthesize heme, and genes for known porphyrin biosynthetic enzymes are not found in the genome sequence of strain V583. (asm.org)
  • We recently described a functional cytochrome bd -type quinol oxidase in E. faecalis V583 ( 27 ). (asm.org)
  • We report that three (EF0089, EF2505 and EF1896, renamed here Fss1, Fss2 and Fss3, respectively, for Enterococcus faecalis surface protein) of the recently predicted MSCRAMMs (microbial surface components recognizing adhesive matrix molecules) in E. faecalis strain V583 bind fibrinogen (Fg). (nih.gov)
  • The complete genome sequence of Enterococcus faecalis V583, a vancomycin-resistant clinical isolate, revealed that more than a quarter of the genome consists of probable mobile or foreign DNA. (jcvi.org)
  • Then, 500 µl of overnight-grown E. faecalis V583 in brain heart infusion (BHI) medium was mixed with 10 ml of BHI medium and 10 ml of filtrate. (asm.org)
  • 10 µl of the enriched filtrate was spotted on bacterial lawns of E. faecalis V583, and after 24 h of incubation at 37°C, clear plaques were observed. (asm.org)
  • Enterococci grown in serum-supplemented broth adhere to Fg-coated surfaces, and inactivation in strain OG1RF of the gene encoding Fss2 resulted in reduced adherence, whilst complementation of the mutant restored full Fg adherence. (nih.gov)
  • Draft Genome Sequence of Enterococcus faecalis PC1.1, a Candidate Probiotic Strain Isolated from Human Feces. (jcvi.org)
  • A biofilm-negative transposon mutant was created from an Enterococcus faecalis strain that produces a lot of biofilm. (nih.gov)
  • These results indicate that bop is involved in production of biofilm and probably regulates expression of biofilm in the E. faecalis strain tested. (nih.gov)
  • Although factors promoting E. faecalis colonization of intestines are not fully known, one implicated pathway is a phosphotransferase system (PTS) in E. faecalis strain OG1RF that phosphorylates gluconate and contains the genes OG1RF_12399 to OG1RF_12402 (OG1RF_12399-12402). (asm.org)
  • In the crystal structure of HPr from E. faecalis, a torsion strain of the backbone at position 16 was observed, which was assumed to be important to the catalytic mechanism. (uni-regensburg.de)
  • Here, using mutation analyses and complementation, we demonstrated that both the ebp (encoding endocarditis and biofilm-associated pili) operon and srtC are important for biofilm production of E. faecalis strain OG1RF. (tmc.edu)
  • Determine the suitability of a non-pathogenic Enterococcus faecalis strain as an alternative to Escherichia coli for in vivo efficacy evaluation of alcohol-based handrubs (ABHR). (biomedcentral.com)
  • We present a case of recurrent postoperative endophthalmitis caused by the same strain of E. faecalis , which was identified by electrophoresis of genomic DNA. (ekjo.org)
  • In the present case, the E. faecalis isolated from the two different specimens were of the same strain. (ekjo.org)
  • Patients with E. faecalis bacteremia were identified through a review of blood-culture records at the hospital microbiology laboratory. (scielo.br)
  • We demonstrate that the commensal bacterium Enterococcus faecalis contributes to the pathogenesis of anastomotic leak through its capacity to degrade collagen and to activate tissue matrix metalloproteinase 9 (MMP9) in host intestinal tissues. (sciencemag.org)
  • Enterococcus faecalis is a commensal Gram-positive pathogen found in the intestines of mammals and is also a leading cause of severe infections occurring mainly among antibiotic-treated dysbiotic hospitalized patients. (asm.org)
  • Enterococcus faecalis is a multifaceted microorganism known to act as a beneficial intestinal commensal bacterium. (biomedcentral.com)
  • In contrast to the much studied extracellular electron transfer mediated by cell surface exposed cytochromes, little is known about components and mechanisms for such electron transfer in organisms without these cytochromes and in gram-positive bacteria, like E. faecalis which is a commensal gut lactic acid bacterium and opportunistic pathogen. (dtu.dk)
  • Enterococcus faecalis , traditionally considered a harmless commensal of the intestinal tract, is now ranked among the leading causes of nosocomial infections. (biomedcentral.com)
  • In an attempt to gain insight into the genetic make-up of commensal E. faecalis , we have studied genomic variation in a collection of community-derived E. faecalis isolated from the feces of Norwegian infants. (biomedcentral.com)
  • The observed correlation between CGH and MLST presented here, may offer a method for the identification of lineage-specific genes, and may therefore add clues on how to distinguish pathogenic from commensal E. faecalis . (biomedcentral.com)
  • Peptide-mediated communication between commensal streptococci and E. faecalis carrying pheromone-responsive plasmids may facilitate conjugative DNA transfer to bystander species, and influence the reservoir of antibiotic resistance determinants of enterococcal origin in the oral metagenome. (microbiologyresearch.org)
  • Bacteremia with E. faecalis is usually the result of infections from other sites, like the urinary tract. (livestrong.com)
  • Among them only two, that is, Enterococcus faecium and Enterococcus faecalis, are now being increasingly recognized to be involved in human infections such as bacteremia, endocarditis, urinary tract infections, and surgical site infections [ 1 , 2 ]. (hindawi.com)
  • Factors associated with IE were studied retrospectively in a population-based cohort of patients with monomicrobial Enterococcus faecalis bacteremia (MEFsB). (springer.com)
  • We therefore aimed to improve the NOVA score using easy accessible clinical parameters in a population-based cohort of patients with monomicrobial E. faecalis bacteremia (MEFsB) from southern Sweden and to externally validate the NOVA score and the improved score in another cohort of patients from a tertiary referral center in another part of Sweden. (springer.com)
  • Enterococcus spp bacteremia is associated with high mortality and the appearance of high-level gentamicin resistance (HLGR) created additional challenges for the treatment of these infections. (scielo.br)
  • Patients with bacteremia due to E. faecalis diagnosed between January 1999 and December 2003 were included in the study. (scielo.br)
  • Enterococcus spp is the third most frequent cause of hospital-acquired bacteremia, accounting for 8% of all nosocomial bacteremias in the United States (1). (scielo.br)
  • Since Enterococcus spp is the second most common cause of Gram-positive blood stream infections in Brazil and that approximately 76% of all enterococcal infections are due to Enterococcus faecalis , we evaluated cases of bacteremia caused by this organism at the teaching hospital of the Universidade Estadual de Campinas (Unicamp), Campinas, São Paulo State (8). (scielo.br)
  • In accordance with current guidelines, E. faecalis bacteremia was defined as at least one positive blood culture for this pathogen (9). (scielo.br)
  • Enterococcus faecalis accounts for the majority of enterococcal bacteremia with urinary tract infections reported as a frequent point of infection. (omicsonline.org)
  • Aim: To investigate different epidemiological characteristics and hospital procedures as potential risk factors for the development of UTI-related bacteremia due to E. faecalis. (omicsonline.org)
  • Methods: In a retrospective case control study, performed in the Capital Region of Denmark between 2010 and 2013, we compared 51 patients, with UTI-related bacteremia due to E. faecalis, to 56 patients, with urinary tract infections due to E. faecalis but without bacteremia, through univariate and multivariate analyses. (omicsonline.org)
  • In the multivariate analysis, male-sex and urological surgery were found to be independent significant predictors of UTI-related bacteremia due to E. faecalis. (omicsonline.org)
  • Conclusion: Male sex, urological surgery and catheterization are factors associated with UTI-related bacteremia due to E. faecalis. (omicsonline.org)
  • The emergence of E. faecalis as a common cause of bacteremia, emphasize the need for more knowledge about UTI-related bacteremia due to E. faecalis . (omicsonline.org)
  • Previous studies have investigated UTI-related bacteremia in general and enterococcal bacteremia, but none have investigated UTI-related bacteremia due to E. faecalis specifically. (omicsonline.org)
  • The aim of this retrospective Danish population-based case-control study is to investigate a number of potential risk factors for UTI-related bacteremia due to E. faecalis . (omicsonline.org)
  • This retrospective case-control study was done for patients with UTI-related bacteremia due to E. faecalis in the Capital Region of Denmark between 2010 and 2013. (omicsonline.org)
  • Enterococci rank among leading cause of nosocomial bacteremia and urinary tract infection in hospital and community acquired infections. (eurekamag.com)
  • To describe an outbreak of bacteremia caused by vancomycin-sensitive Enterococcus faecalis (VSEfe). (edu.au)
  • Important clinical infections caused by Enterococcus include urinary tract infections (see Enterococcus faecalis), bacteremia, bacterial endocarditis, diverticulitis, meningitis, and spontaneous bacterial peritonitis. (wikipedia.org)
  • [1] [2] Like other species in the genus Enterococcus , E. faecalis is found in healthy humans, but can cause life-threatening infections, especially in the nosocomial (hospital) environment, where the naturally high levels of antibiotic resistance found in E. faecalis contribute to its pathogenicity. (wikipedia.org)
  • [3] Re-infected root canal-treated teeth are about nine times more likely to harbor E. faecalis than cases of primary infections. (wikipedia.org)
  • E. faecalis is found in most healthy individuals, but can cause endocarditis and sepsis , urinary tract infections (UTIs), meningitis , and other infections in humans. (wikipedia.org)
  • [6] [7] Several virulence factors are thought to contribute to E. faecalis infections. (wikipedia.org)
  • As an opportunistic pathogen, E. faecalis can cause life-threatening infections, especially in the nosocomial (hospital) environment, where the naturally high levels of antibiotic resistance found in E. faecalis contribute to its pathogenicity. (wikipedia.org)
  • Enterococcus faecalis has been implicated in a wide variety of human infections and is a notorious problem in hospital-acquired infections. (livestrong.com)
  • The infection most commonly caused by E. faecalis is urinary tract infections (UTI). (livestrong.com)
  • Most antibiotics used for systemic infections singly that are effective against other types of Gram-positive cocci (staphylococci and streptococci) are ineffective against E. faecalis. (livestrong.com)
  • However, E. faecalis can cause disease in, e.g., immunodeficient persons, and it is frequently the causative agent of nosocomial infections. (asm.org)
  • Enterococcus faecalis appears in many tooth root infections and is not eliminated by root canal therapy. (ovid.com)
  • Among enterococci, Enterococcus faecalis occurs ubiquitously, with the highest incidence of human and animal infections. (asm.org)
  • Enterococci are part of the normal human gut flora and can cause severe infections including urinary tract infections, gastrointestinal infections, catheter-related infections, and infective endocarditis (IE). (springer.com)
  • In Brazil, clinical and epidemiological studies of enterococcal infections have been less comprehensive, with most studies focusing on vancomycin-resistant Enterococcus (VRE), which is still restricted to a few Brazilian hospitals (6-8). (scielo.br)
  • Pulsed-field gel electrophoresis of Sma I-digested Enterococcus faecalis isolated from humans with urinary tract infections and from poultry in the same houselhold, Vietnam, January 2008-January 2010. (cdc.gov)
  • To investigate the risk factors, clinical features, molecular epidemiology, and treatment outcomes associated with an outbreak of infections due to beta-lactamase-producing, high-level gentamicin-resistant Enterococcus faecalis . (annals.org)
  • It could be a promising therapeutic target in control programs for the eradication of persistent infections associated with the presence of E faecalis in biofilm. (isciii.es)
  • During the last decades Enterococcus species have become of increasing medical interest as human pathogens causing an increasing number of infections in both Europea and the US [ 1 - 3 ]. (omicsonline.org)
  • Is the Subject Area "Enterococcus infections" applicable to this article? (plos.org)
  • Information about vertical transmission and subsequent spreading and colonization of the cloacal mucosa through cloacal 'drinking' during hatch are important to understand the epidemiology of E. faecalis infections. (dtu.dk)
  • Enterococcus faecalis is recognized as one of the leading pathogens causing nosocomial infections. (physiciansweekly.com)
  • Enterococci are important nosocomial pathogens, with Enterococcus faecalis most commonly responsible for human infections. (k-state.edu)
  • Previous MLST analysis of E. faecalis have identified so-called high-risk enterococcal clonal complexes (HiRECC), defined as genetically distinct subpopulations, epidemiologically associated with enterococcal infections. (biomedcentral.com)
  • During the last three decades, enterococci have emerged as important pathogens and as a major cause of nosocomial infections. (biomedcentral.com)
  • On Western blotting (immunoblotting), they were detected strongly by immunoglobulin G (IgG) in sera from patients with E. faecalis endocarditis, but not in sera from patients with other E. faecalis infections or with endocarditis due to other streptococci. (mysciencework.com)
  • Of 10 cases of endocarditis due to other streptococci and 10 E. faecalis infections other than endocarditis, 9 and 8, respectively, gave negative results. (mysciencework.com)
  • Though the bacterial opportunist Enterococcus faecalis causes a myriad of hospital-acquired infections (HAIs), including catheter-associated urinary tract infections (CAUTIs), little is known about the virulence mechanisms that it employs. (ntu.edu.sg)
  • As this motif occurs in other sortase-assembled pili, our results have implications for the molecular basis of virulence not only in E. faecalis CAUTI but also in additional infections caused by enterococci and other Gram-positive pathogens. (ntu.edu.sg)
  • Enterococcus , a Gram-positive facultative anaerobic cocci belonging to the lactic acid bacteria of the phylum Firmicutes, is known to be able to resist a wide range of hostile conditions such as different pH levels, high concentration of NaCl (6.5%), and the extended temperatures between 5°C and 65°C. Despite being the third most common nosocomial pathogen, our understanding on its virulence factors is still poorly understood. (hindawi.com)
  • Univariate analysis showed older age, higher APACHE II score, nosocomial acquisition, recent surgical procedure, total parenteral nutrition, and antibiotic treatment to be significantly associated with the acquisition of beta-lactamase-producing, high-level gentamicin-resistant E. faecalis . (annals.org)
  • High-level gentamicin resistant (HLGR) enterococci (Enterococcus faecalis and Enterococcus faecium) have become a substantial nosocomial problem in many countries. (diva-portal.org)
  • Enterococcus faecalis (E. faecalis) has become a major leading cause of nosocomial endocarditis. (pasteur.fr)
  • Increasing multidrug resistance in Enterococcus faecalis, a nosocomial opportunist and common cause of bacterial endocarditis, emphasizes the need for alternative therapeutic approaches such as immunotherapy or immunoprophylaxis. (tmc.edu)
  • Our finding of a catalase whose synthesis is dependent on the acquisition of heme in the opportunistic pathogen E. faecalis might be of clinical importance. (asm.org)
  • Ability of clinical laboratories to detect antimicrobial agent- resistant enterococci [published erratum appears in J. Clin. (atcc.org)
  • Our objective was to characterize the epidemiological, clinical, and microbiological features of bacteremias caused by HLGR and non_HLGR E. faecalis isolated from January 1999 through December 2003. (scielo.br)
  • Enterococcus faecalis accounts for 65-90% of clinical Enterococcus spp. (omicsonline.org)
  • Antibiotic-resistance in enterococci influences clinical outcomes drastically. (omicsonline.org)
  • Treatment of multidrug-resistant enterococci has become a challenging clinical problem in hospitals around the world due to the lack of reliable therapeutic options. (rice.edu)
  • This study aimed to detect vanA and vanB genes, virulence factors, and antimicrobial susceptibility of Enterococcus faecalis isolated from clinical specimens in Ribat University Hospital. (alliedacademies.org)
  • The cell wall adhesion (efaAfm) in E. faecium has 66% similarity with endocarditis specific antigen of E. faecalis T8 (Accession: EEU25576). (hindawi.com)
  • Is Once-Daily High-Dose Ceftriaxone plus Ampicillin an Alternative for Enterococcus faecalis Infective Endocarditis in Outpatient Parenteral Antibiotic Therapy Programs? (asm.org)
  • Ceftriaxone administered as once-daily high-dose short infusion combined with ampicillin has been proposed for the treatment of Enterococcus faecalis infective endocarditis in outpatient parenteral antibiotic therapy programs (OPAT). (asm.org)
  • Lack of this information could hinder the inclusion in OPAT of patients suffering from Enterococcus faecalis infective endocarditis treated with ampicillin plus. (asm.org)
  • A case of infective endocarditis from Enterococcus faecalis after colonoscopy in a patient with aortic stenoinsufficiency and bleeding intestinal angiodysplasia (Heyde's syndrome) is reported. (bmj.com)
  • We report a case of infective endocarditis from Enterococcus faecalis after colonoscopy in a patient with aortic stenoinsufficiency and bleeding intestinal angiodysplasia (Heyde's syndrome). (bmj.com)
  • E faecalis makes up a significant portion of the normal gut flora of almost all humans, and enterococci account for approximately 5% to 18% of all cases in most series of infective endocarditis. (bmj.com)
  • Chuang ON, Schlievert PM, Wells CL, Manias DA, Tripp TJ, Dunny GM (2009) Multiple functional domains of Enterococcus faecalis aggregation substance Asc10 contribute to endocarditis virulence. (springer.com)
  • Endocarditis and biofilm-associated pili of Enterococcus faecalis. (tmc.edu)
  • These biologically important surface pili, which are antigenic in humans during endocarditis and encoded by a ubiquitous E. faecalis operon, may be a useful immunotarget for studies aimed at prevention and/or treatment of this pathogen. (tmc.edu)
  • Serological response in Enterococcus faecalis endocarditis det. (mysciencework.com)
  • Serological response in Enterococcus faecalis endocarditis determined by enzyme-linked immunosorbent assay. (mysciencework.com)
  • We developed an enzyme-linked immunosorbent assay system to measure IgG, IgM, and IgA levels to these antigens and evaluated its potential as a serodiagnostic test for E. faecalis endocarditis. (mysciencework.com)
  • The test correctly diagnosed E. faecalis endocarditis in 15 of 16 cases. (mysciencework.com)
  • The test should prove particularly useful in culture-negative cases, for which choice of appropriate antibiotic therapy for E. faecalis endocarditis is vital. (mysciencework.com)
  • However, the endocarditis- and biofilm-associated pilus (Ebp), a member of the sortase-assembled pilus family, was shown to play a role in a mouse model of E. faecalis ascending UTI. (ntu.edu.sg)
  • On the other hand, enterococci, in particular E. faecalis and E. faecium , are important conditional pathogens. (biomedcentral.com)
  • Together, these results demonstrate the involvement of rnjB in E. faecalis pilin gene expression and provide insight into a novel mechanism of regulation of pilus production in Gram-positive pathogens. (tmc.edu)
  • The genus Enterococcus includes 38 species of Gram-positive bacteria, among which E. faecalis and E. faecium are the most abundant pathogens in humans. (asm.org)
  • These two Enterococcus species are ranked among the most common and resistant pathogens in hospitals ( 3 ). (asm.org)
  • Herein, we describe mechanisms that link Enterococcus faecalis, a bacterium known to produce extracellular superoxide, to the acute induction of chromosomal instability. (nih.gov)
  • A scoring method was developed for the diagnosis of E. faecalis and S. gallolyticus IE using the presence of reactivity to at least two antigenic bands for each bacterium and the positivity to at least one of the Ef300, Ef72, or Ef36 proteic bands for E. faecalis , and positivity for the two Sg75 and Sg97 proteic bands for S. gallolyticus . (frontiersin.org)
  • Enterococcus faecalis is a pathogenic gram-positive bacterium closely associated with apical periodontitis. (ovid.com)
  • E. faecalis is an enteric facultative gram positive bacterium which can grow independently in the root canal. (scielo.org.za)
  • Enterococcus faecalis (bacterium incl. (bode-science-center.com)
  • The Gram-positive Enterococcus faecalis bacterium can be observed singly, in pairs or in chains, and belongs to the Enterococcaceae family. (bode-science-center.com)
  • Molecular characterization and multilaboratory evaluation of Enterococcus faecalis ATCC 51299 for quality control of screening tests for vancomycin and high-level aminoglycoside resistance in enterococci. (atcc.org)
  • Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212) and Candida albicans (ATCC 10231). (scielo.br)
  • Test organisms were E. faecalis (ATCC 47077) or E. coli K12 (NCTC 10538). (biomedcentral.com)
  • Like other species in the genus Enterococcus, E. faecalis is found in healthy humans and can be used as probiotic. (wikipedia.org)
  • In this work, we describe a unique series of posttranscriptional regulatory strategies that influence expression of ethanolamine utilization genes ( eut ) in Enterococcus , Clostridium , and Listeria species. (pnas.org)
  • In conclusion, the pathogenicity of E. faecalis and E. faecium could be associated with different virulence factors and these genes are widely distributed among the enterococcal species. (hindawi.com)
  • That is, E faecalis may be the pathogenic determinant for any particular peri-implantitis infection of a multiple-species infection. (ovid.com)
  • Of the 11 antibiotics tested, E. faecalis was most commonly resistant to tetracycline, doxycycline, streptomycin, gentamicin, and erythromycin, and these traits were intra-species horizontally transferrable by in vitro conjugation. (k-state.edu)
  • E. faecalis /pAM373/pAMS470 cells were incubated with synthetic s.g.cAM373, reverse peptide s.g.cAM373-R, or peptide-free medium and examined for their abilities to transfer plasmid DNA to streptococcal species in the presence of DNase. (microbiologyresearch.org)
  • In other words, the culture rate of S. epidermidis , or coagulase negative staphylococci, has decreased, and the culture rate of the Enterococcus species has increased tenfold compared to previous reports [ 1 , 2 , 3 ]. (ekjo.org)
  • Several Enterococcus species are mentioned in journal reports of endophthalmitis recurrence after cataract surgery [ 4 ]. (ekjo.org)
  • Coloured scanning electron micrograph (SEM) of a clump of Enterococcus faecalis bacteria. (sciencephoto.com)
  • Although sodium hypochlorite (NaOCl) has been used as a common endodontic irrigant to eradicate bacteria in the root canal, it has not been elucidated whether NaOCl attenuates the inflammatory response induced by the E. faecalis virulence factor lipoteichoic acid (EfLTA). (ovid.com)
  • Like most intestinal bacteria, E. faecalis does not synthesize heme (in this report, heme refers to iron protoporphyrin IX regardless of the iron redox state). (asm.org)
  • Here, we report a new mechanism used by E. faecalis to sense heme and trigger the synthesis of a heme efflux pump that balances the amount of heme inside the bacteria. (asm.org)
  • We found that among prolonged-stay patients admitted to ICUs, the rectal flora was altered, with a decrease in Gram-negative rods in favour of Gram-positive bacteria, mainly Coagulase negative staphylococci and enterococci. (diva-portal.org)
  • This study aimed to investigate the antimicrobial activity of Chamaecyparis obtusaextract on Enterococcus faecalis (E. faecalis), typical causative bacteria that causes endodontic treatment failure and pulpitis. (alliedacademies.org)
  • Profiles of E. faecalis (prevalence, antibiotic resistance, and virulence traits) from each of WWTF sludge and associated house flies were similar, indicating that flies successfully acquired these bacteria from this substrate. (k-state.edu)
  • The evaluation of data obtained indicate a stable and well-defined structure of HPr from E. faecalis, with an overall fold similar to that found in HPr from other bacteria. (uni-regensburg.de)
  • Our results demonstrate that aPDT mediated by the three above-mentioned PS was effective in eliminating both gram-positive bacteria, although P. acnes showed remarkably higher susceptibility to aPDT when compared to E. faecalis. (fapesp.br)
  • Enterococcus is a large genus of lactic acid bacteria of the phylum Firmicutes. (wikipedia.org)
  • Quantitation of biofilm development by E. faecalis OG1RF in the chemostat-based biofilm fermentor. (asm.org)
  • Biofilm development by E. faecalis OG1RF in the wells of microtiter plates. (asm.org)
  • With these Ped r mutants of E. faecalis there was: a gradual neutralization in cell wall surface charge involving D-alanylation of wall teichoic acids (WTA) and lipoteichoic acids (LTA), increase in cell-surface hydrophobicity, increased cell aggregation and biofilm formation and ultra-structural changes in the cell wall, and a reduction of periplasmic space. (springer.com)
  • Enterococcus faecalis biofilm traits and distribution characteristics in China have not been clarified. (frontiersin.org)
  • The system consisted of a previously extracted human tooth that was cut and prepared to provide by means of its root canal, an adequate surface for biofilm formation on behalf of Enterococcus faecalis. (isciii.es)
  • The expression of Esp gene is associated with biofilm formation in E. faecalis and in the adherence to abiotic surfaces. (isciii.es)
  • Chuang-Smith O, Wells C, Henry-Stanley M, Dunny GM (2010) Acceleration of Enterococcus faecalis biofilm formation by aggregation substance expression in an ex vivo model of cardiac valve colonization. (springer.com)
  • Genomic Insights into Biofilm-Forming Enterococcus faecalis SK460 Isolated from a Chronic Diabetic Ulcer Patient. (physiciansweekly.com)
  • Enterococcus faecalis rnjB is required for pilin gene expression and biofilm formation. (tmc.edu)
  • citation needed] Enterococci are able to form biofilm in the prostate gland, making their eradication difficult. (wikipedia.org)
  • The small alarmone synthetase RelQ from the Gram-positive pathogen Enterococcus faecalis enzymatically regulates the levels of alarmone nucleotide (p)ppGpp, a key regulator of stress adaptation, pathogenicity, and antibiotic tolerance. (pnas.org)
  • We show that the tetrameric small alarmone synthetase (SAS) RelQ from the Gram-positive pathogen Enterococcus faecalis is a sequence-specific RNA-binding protein. (pnas.org)
  • and in particular E. faecalis is recognized as an important human pathogen causing high levels of morbidity and mortality as well as extensive economic costs for hospitals. (omicsonline.org)
  • E. faecalis demonstrates strong potential as a test organism that represents a pathogen with high environmental stability known to be spread by contaminated hands in healthcare settings. (biomedcentral.com)
  • Desouky SE, Nishiguchi K, Zendo T, Igarashi Y, Williams P, Sonomoto K, Nakayama J (2013) High-throughput screening of inhibitors targeting Agr/Fsr quorum sensing in Staphylococcus aureus and Enterococcus faecalis . (springer.com)
  • E. coli FtsZ, Staphylococcus aureus FtsZ, Enterococcus faecalis FtsZ, Streptocuccus pneumoniae Fts Z proteins. (cytoskeleton.com)
  • These results indicate that platelet activation and aggregation by E. faecalis is dependent on both host and bacterial factors and that it may be involved in the pathogenesis of invasive disease with this organism. (lu.se)
  • The presence of OG1RF_12399-12402 is not required for E. faecalis colonization of the mouse intestine but is associated with an accelerated onset of experimental colitis in interleukin-10-deficient mice, altered bacterial composition in the colon, enhanced E. faecalis survival within macrophages, and increased proinflammatory cytokine secretion by colon tissue and macrophages. (asm.org)
  • Further studies of bacterial carbohydrate metabolism in general, and E. faecalis PTS-gluconate in particular, during inflammation may identify new mechanisms of disease pathogenesis. (asm.org)
  • New epidemiological evidence has shown that enterococci are major infectious agent in chronic bacterial prostatitis. (wikipedia.org)
  • Engelbert M, Mylonakis E, Ausubel FM, Calderwood SB, Gilmore MS (2004) Contribution of gelatinase, serine protease, and fsr to the pathogenesis of Enterococcus faecalis endophthalmitis. (springer.com)
  • A detailed understanding of the molecular basis of Enterococcus faecalis pathogenesis in CAUTI is necessary for the development of preventative and therapeutic strategies. (ntu.edu.sg)
  • 2002). The Enterococci: Pathogenesis, Molecular Biology, and Antibiotic Resistance. (wikipedia.org)
  • DIAZ CABALLERO, A. e SIMANCAS PALLARES, M. . Gene expression esp (enterococcus surface protein) of enterococcus faecalis in an in vitro model of extracted teeth . (isciii.es)
  • Mylonakis E, Engelbert M, Qin X, Sifri CD, Murray BE, Ausubel FM, Gilmore MS, Calderwood SB (2002) The Enterococcus faecalis fsrB gene, a key component of the fsr quorum sensing system, is associated with virulence in the rabbit endophthalmitis model. (springer.com)
  • Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli . (asm.org)
  • Enterococcus faecalis rnjB is required for pilin gene expression and b" by Peng Gao, Kenneth L Pinkston et al. (tmc.edu)
  • This study demonstrates in vitro conjugative transfer of the esp gene among E. faecium and E. faecalis . (oup.com)
  • E. faecalis is usually resistant to many commonly used antimicrobial agents ( aminoglycosides , aztreonamand quinolones . (wikipedia.org)
  • A less nephrotoxic combination of ampicillin and ceftriaxone (even though E. faecalis is resistant to cephalosporins, ceftriaxone is working synergistically with ampicillin) may be used alternatively for ampicillin-susceptible E. faecalis . (wikipedia.org)
  • The fact that Enetrococcus faecalis can be resistant to a wide array of antibiotics must contribute to the proliferation of the organism. (livestrong.com)
  • In this direction, we selected stable and gradual dose-dependent pediocin PA-1 single exposure resistant (Ped r ) mutants of E. faecalis , which conferred cross-protection to diverse CAMPs, viz. (springer.com)
  • Sixty-five patients infected or colonized with beta-lactamase-producing high-level gentamicin-resistant E. faecalis (case patients) were matched and compared with 65 randomly selected patients infected or colonized with beta-lactamase-negative, gentamicin-susceptible E. faecalis . (annals.org)
  • Daptomycin (DAP), a cell membrane-targeting cationic antimicrobial lipopeptide, is the only antibiotic with in vitro bactericidal activity against vancomycin-resistant enterococci (VRE). (rice.edu)
  • Previously, we demonstrated that development of DAP resistance in vancomycin-resistant Enterococcus faecalis is associated with mutations in genes encoding proteins with two main functions, (i) control of the cell envelope stress response to antibiotics and antimicrobial peptides (LiaFSR system) and (ii) cell membrane phospholipid metabolism (glycerophosphoryl diester phosphodiesterase and cardiolipin synthase). (rice.edu)
  • DAP septal diversion by DAP-resistant E. faecalis is mediated by initial redistribution of cell membrane cardiolipin-rich microdomains associated with a single amino acid deletion within the transmembrane protein LiaF (a member of a three-component regulatory system [LiaFSR] involved in cell envelope homeostasis). (rice.edu)
  • This study demonstrates that house flies acquire antibiotic-resistant enterococci from WWTF and potentially disseminate them to the surrounding environment. (k-state.edu)
  • The farm-level prevalence of drug-resistant E. coli, E. faecalis, and Salmonella spp. (elsevier.com)
  • Inherently resistant to several classes of antibiotics and with a propensity to acquire vancomycin resistance, enterococci are particularly worrisome etiologic agents of CAUTI. (ntu.edu.sg)
  • Enterococcus faecalis can be resistant to aminopenicillins, glycopeptides, vancomycin and teicoplanin (= VRE, vancomycin-resistant enterococci) and increasingly also to new antibiotics such as cephalosporines or fluoroquinolones. (bode-science-center.com)
  • Some enterococci are intrinsically resistant to β-lactam-based antibiotics (penicillins, cephalosporins, carbapenems), as well as many aminoglycosides. (wikipedia.org)
  • [2] [ verification needed ] E. faecalis has been frequently found in reinfected, root canal-treated teeth in prevalence values ranging from 30% to 90% of the cases. (wikipedia.org)
  • The current study was aimed to determine the prevalence of different virulence genes in Enterococcus faecalis and Enterococcus faecium . (hindawi.com)
  • The prevalence of E. faecalis increased from 14% at 0 h to 97% after 24 h for the Brown Lohmann chickens and from 0.5% to 23% for the White Lohmann flock. (dtu.dk)
  • The prevalence of glycopeptides, aminoglycosides and erythromycin resistance among Enterococcus faecalis and Enterococcus faecium was investigated. (semanticscholar.org)
  • 1. Sedgley C , Buck G , Appelbe O . Prevalence of Enterococcus faecalis at multiple oral sites in endodontic patients using culture and PCR. (microbiologyresearch.org)
  • Prevalence, phenotype and genotype of oral enterococci. (microbiologyresearch.org)
  • Inactivation of GalU leads to a cell wall-associated polysaccharide defect that reduces the susceptibility of Enterococcus faecalis to bacteriolytic agents. (asm.org)
  • Susceptibility of Enterococcus faecalis and Propio. (fapesp.br)
  • For instance, pilA in E. faecium has 99% similarity with the cell wall surface anchor family protein of Enterococcus sp. (hindawi.com)
  • The pilB of E. faecium has 74% similarity with the cell wall surface anchor protein of E. faecalis B318 (Accession: ETU21897). (hindawi.com)
  • Meanwhile, the ecbA in E. faecium has 92% similarity with the cell wall surface anchor protein of E. faecalis (Accession: WP_010708790) and 91% with Cna protein B-type domain protein E. faecalis (Accession: WP_002417581) (these regions are part of a collagen binding MSCRAMM, ecbA). (hindawi.com)
  • With regard to the collagen binding MSCRAMM Acm (fms8) in E. faecium , 57% similarity has been observed with the collagen binding domain protein of E. faecalis (Accession: WP_016632409). (hindawi.com)
  • Finally, 37% similarity of the cell wall anchored protein sgrA in E. faecium has been detected when compared to the surface adhesion protein in Enterococcus sp. (hindawi.com)
  • Partial proteolysis of AtlA was detected in vivo: zymograms of E. faecalis extracts revealed two catalytically active protein bands of 62 and 72 kDa that were both absent in extracts from an atlA null mutant. (inserm.fr)
  • Based on the complete sequential assignment of the 1H-NMR spectrum by multidimensional NMR techniques the secondary structure and the local geometry of the active site of histidine-containing phosphocarrier protein (HPr) from Enterococcus faecalis were elucidated. (uni-regensburg.de)
  • Utilizing a monoclonal antibody developed against the Enterococcus faecalis major pilus protein EbpC, we identified mutants from a transposon (Tn) insertion library which lack surface-exposed Ebp pili. (tmc.edu)
  • Our results indicate a high frequency of E. faecalis process vancomycin resistance with vanB genotype and high frequencies of gelE, asa1, and hyl virulence genes. (alliedacademies.org)
  • Escherichia coli, Enterococcus faecalis) and antimicrobial resistance testing. (elsevier.com)
  • Assessment of Tedizolid In Vitro Activity and Resistance Mechanisms against a Collection of Enterococcus spp. (asm.org)
  • Antimicrobial activity of Ca(OH)2 containing pastes with Enterococcus faecalis in vitro. (biomedsearch.com)
  • Limited digestion of AtlA by proteinase K in vitro suggested that the proteolytic cleavage of AtlA in E. faecalis extracts corresponds to the truncation of the N-terminal domain, which is rich in threonine and glutamic acid residues. (inserm.fr)
  • High purity recombinant endo-alpha-N-Acetylgalactosaminidase (E. faecalis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (creative-enzymes.com)
  • Enterococcus faecalis may cause catheter-associated urinary tract infection or wound infection. (bode-science-center.com)
  • Similar to the first infection, E. faecalis was isolated from the vitreous specimen. (ekjo.org)
  • One of the most important and serious factors about Enterococcus faecalis and the other enterococci is its resistance to a variety of antibiotics. (livestrong.com)
  • Treating E. faecalis UTI's are usually easier to treat with antibiotics. (livestrong.com)
  • Due to innate and acquired resistance in Enterococcus faecalis against most antibiotics, identification of new alternatives has increased interest in diverse populations of potent cationic antimicrobial peptides (CAMPs) for treatment and natural food biopreservation. (springer.com)
  • High-level gentamicin resistance (HLGR) has emerged among Enterococcus spp over the last two decades, and is associated with the loss of synergy between cell-active antibiotics (e.g., vancomycin and penicillin, particularly ampicillin) and most aminoglycosides (e.g., gentamicin, tobramycin, amikacin, and kanamycin), thus posing additional treatment challenges (2). (scielo.br)
  • It is noteworthy that E. faecalis and E. faecium cause treatment to be increasing difficult because of their intrinsic and acquired resistance to many antibiotics. (frontiersin.org)
  • IMPORTANCE Enterococcus faecalis , a normal and harmless colonizer of the human intestinal flora can cause severe infectious diseases in immunocompromised patients, particularly those that have been heavily treated with antibiotics. (asm.org)
  • The purpose of this thesis was to study enterococci in Sweden, their resistance to antibiotics in general and high-level gentamicin resistance (HLGR) in particular, with a special focus on the ICU setting. (diva-portal.org)
  • In contrast, the standard recommended intravenous antibiotics used in patients undergoing colorectal surgery did not eliminate E. faecalis at anastomotic tissues nor did they prevent leak in our rat model. (igsb.org)
  • Enterococcus faecalis, as the name implies, is found normally in the intestines of humans, animals and birds. (livestrong.com)
  • Enterococci are considered to be part of normal gut microbiota in both humans and animals and capable to survive in a diverse range of harsh conditions. (hindawi.com)
  • Enterococci constitute an integral part of the intestinal flora of many invertebrates, birds and mammals including humans. (biomedcentral.com)
  • Immortalized human and nontransformed murine colonic epithelial cells, along with a mouse colonic ligation model, were used to assess the effect of E. faecalis on genomic DNA stability and damage. (nih.gov)
  • The aim of this study was to investigate the genomic diversity among fecal E. faecalis isolated from healthy Norwegian infants by means of CGH. (biomedcentral.com)
  • What makes Enterococcus faecalis pathogenic is less clear than with many other microorganisms. (livestrong.com)
  • Canadian researchers have published a study assessing the antimicrobial resistance and virulence of genotypes of Enterococcus faecalis, taken from samples taken from a commercial pork processing plant. (pigprogress.net)
  • The objective of the study was to assess the antimicrobial resistance and virulence genotypes of Enterococcus faecalis isolated from samples obtained from a commercial pork processing plant. (pigprogress.net)
  • Enterococcus faecalis cells cannot synthesize porphyrins and do not rely on heme for growth but can take up heme and use it to synthesize heme proteins. (asm.org)
  • The Bac41 effector consists of the secreted proteins BacL 1 and BacA, which attack the cell wall of the target E. faecalis cell to induce bacteriolysis. (asm.org)
  • In addition, immunogold electron microscopy using antisera against EbpA-EbpC proteins as well as patient serum demonstrated that E. faecalis produces pleomorphic surface pili. (tmc.edu)
  • Because Enterococcus faecalis is normally found in the intestinal tract of people, it easily becomes a contamination problem in hospital and institutional settings. (livestrong.com)
  • These findings provide novel insights into mechanisms by which E. faecalis and intestinal commensals can contribute to cellular transformation and tumorigenesis. (nih.gov)
  • We hypothesize that this PTS permits growth in gluconate, facilitates E. faecalis intestinal colonization, and exacerbates colitis. (asm.org)
  • Here we report a draft genome sequence of Enterococcus faecalis SK460, isolated from a chronic diabetic foot ulcer patient. (physiciansweekly.com)
  • Characterization of Aminoglycoside Resistance and Virulence Genes among Enterococcus spp. (semanticscholar.org)
  • Enterococcal plasmid-encoded bacteriolysin Bac41 is a selective antimicrobial system that is considered to provide a competitive advantage to Enterococcus faecalis cells that carry the Bac41-coding plasmid. (asm.org)
  • Molecular analysis revealed a plasmid localization of esp in the E. faecium transconjugant and chromosome-to-chromosome transfer in E. faecalis . (oup.com)
  • Three blood specimens for culture were drawn and within seven days all cultures were positive for Gram positive cocci, identified as ampicillin and high level gentamicin susceptible E faecalis . (bmj.com)
  • Thus, this study identified the Ebp pilus as a virulence factor in E. faecalis CAUTI and also defined the molecular basis of this function, critical knowledge for the rational development of targeted therapeutics. (ntu.edu.sg)
  • Multiplex PCR for detection of aminoglycoside resistance genes in enterococci. (semanticscholar.org)
  • Ers has been qualified as the PrfA-like transcriptional regulator of Enterococcus faecalis . (archives-ouvertes.fr)
  • Taken together, these results indicate that the regulator Ers has a pleiotropic effect, especially in the cellular metabolism and virulence of E. faecalis . (archives-ouvertes.fr)
  • The rationale for the retraction of Synercid's indication for VRE was based upon poor efficacy in E. faecalis, which is implicated in the vast majority of VRE cases. (wikipedia.org)
  • Enterococci are Gram-positive facultative anaerobic cocci with a low GC-content. (biomedcentral.com)
  • Enterococci are facultative anaerobic organisms, i.e., they are capable of cellular respiration in both oxygen-rich and oxygen-poor environments. (wikipedia.org)
  • Two of five E. faecalis and five of nine E. faecium transferred antibiotic resistance determinants into a recipient. (oup.com)
  • One isolate from each E. faecalis -positive sample was analyzed for antimicrobial susceptibility and characterized using a enterococcal microarray for analysis of resistance and virulence genes. (pigprogress.net)
  • however, these results underscore the importance of E. faecalis as a reservoir of resistance and virulence genes. (pigprogress.net)
  • For maintenance and routine genetic work, E. faecalis was grown in Todd-Hewitt (TH) broth (Difco) or brain heart infusion broth (Difco) or on TH agar plates. (asm.org)
  • The high genetic plasticity of E. faecalis complicates both molecular investigations and phylogenetic analyses. (asm.org)