The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
Embryonic and fetal development that takes place in an artificial environment in vitro.
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
Insulated enclosures in which temperature, humidity, and other environmental conditions can be regulated at levels optimal for growth, hatching, reproduction, or metabolic reactions.
An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.
Morphological and physiological development of EMBRYOS.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
An agent that causes the production of physical defects in the developing embryo.
Morphological and physiological development of EMBRYOS or FETUSES.
Methods for maintaining or growing CELLS in vitro.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Techniques used in studying bacteria.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Congenital abnormalities caused by medicinal substances or drugs of abuse given to or taken by the mother, or to which she is inadvertently exposed during the manufacture of such substances. The concept excludes abnormalities resulting from exposure to non-medicinal chemicals in the environment.
Endometrial implantation of EMBRYO, MAMMALIAN at the BLASTOCYST stage.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
The fertilized OVUM resulting from the fusion of a male and a female gamete.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
An early embryonic developmental process of CHORDATES that is characterized by morphogenic movements of ECTODERM resulting in the formation of the NEURAL PLATE; the NEURAL CREST; and the NEURAL TUBE. Improper closure of the NEURAL GROOVE results in congenital NEURAL TUBE DEFECTS.
The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
The potential of the FETUS to survive outside the UTERUS after birth, natural or induced. Fetal viability depends largely on the FETAL ORGAN MATURITY, and environmental conditions.
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
The techniques used to select and/or place only one embryo from FERTILIZATION IN VITRO into the uterine cavity to establish a singleton pregnancy.
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).
Clinical and laboratory techniques used to enhance fertility in humans and animals.
The ratio of the number of conceptions (CONCEPTION) including LIVE BIRTH; STILLBIRTH; and fetal losses, to the mean number of females of reproductive age in a population during a set time period.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
The first of four extra-embryonic membranes to form during EMBRYOGENESIS. In REPTILES and BIRDS, it arises from endoderm and mesoderm to incorporate the EGG YOLK into the DIGESTIVE TRACT for nourishing the embryo. In placental MAMMALS, its nutritional function is vestigial; however, it is the source of INTESTINAL MUCOSA; BLOOD CELLS; and GERM CELLS. It is sometimes called the vitelline sac, which should not be confused with the VITELLINE MEMBRANE of the egg.
A vasoconstrictor found in ergot of Central Europe. It is a serotonin agonist that has been used as an oxytocic agent and in the treatment of MIGRAINE DISORDERS.
A serotonin antagonist and a histamine H1 blocker used as antipruritic, appetite stimulant, antiallergic, and for the post-gastrectomy dumping syndrome, etc.
The event that a FETUS is born alive with heartbeats or RESPIRATION regardless of GESTATIONAL AGE. Such liveborn is called a newborn infant (INFANT, NEWBORN).
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
The structure that forms the roof of the mouth. It consists of the anterior hard palate (PALATE, HARD) and the posterior soft palate (PALATE, SOFT).
Elements of limited time intervals, contributing to particular results or situations.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Techniques used in microbiology.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
Results of conception and ensuing pregnancy, including LIVE BIRTH; STILLBIRTH; SPONTANEOUS ABORTION; INDUCED ABORTION. The outcome may follow natural or artificial insemination or any of the various ASSISTED REPRODUCTIVE TECHNIQUES, such as EMBRYO TRANSFER or FERTILIZATION IN VITRO.
A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Malformations of organs or body parts during development in utero.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Early pregnancy loss during the EMBRYO, MAMMALIAN stage of development. In the human, this period comprises the second through eighth week after fertilization.
The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated as the time from the last day of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells.
A series of steps taken in order to conduct research.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Infections by bacteria, general or unspecified.
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Utilization or disposal of an embryo that is fertilized but not immediately transplanted and resulting course of action.
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

Evaluation of bovine embryos produced in high performance serum-free media. (1/644)

This review evaluates the quality of bovine embryos developed from in vitro-matured (IVM) and -fertilized (IVF) oocytes cultured in either serum-free or serum-containing media. Bovine embryos cultured in serum-supplemented medium contain numerous cytoplasmic lipid droplets and immature mitochondria compared to those cultured in serum-free medium. The accumulation of cytoplasmic lipids in embryos developed in serum-containing medium may be a result of incorporation of lipoproteins from the serum and may result in impaired function of mitochondria. The improved serum-free media (IVMD101 and IVD101) offer several advantages over culture in serum-containing medium, including increased rates of blastocyst formation and higher cell numbers. Additionally, the survival and hatching rates of embryos produced in serum-free media after post-thaw culture were superior to those of embryos produced in the serum-containing medium, suggesting that the abnormal accumulation of cytoplasmic lipids in embryos may have a negative effect on the sensitivity of embryos to chilling and freezing. These serum-free culture systems have proven to be beneficial for the production of good quality embryos from IVM-IVF bovine oocytes. Furthermore, recent studies have shown a correlation between mitochondrial function (oxygen consumption) and embryo quality. A new method using scanning electrochemical microscopy may be capable of assessing the viability and developmental potential of bovine embryos.  (+info)

Effects of BSA and fetal bovine serum in culture medium on development of rat embryos. (2/644)

Rat 1-cell embryos, recovered from naturally mated females, were cultured in a chemically defined medium (mR1ECM) or in mR1ECM supplemented with BSA (4 mg/ml; mR1ECM-BSA) or fetal bovine serum (FBS; 10%, v:v; mR1ECM-FBS) instead of polyvinylalcohol. There was no difference in percentages of embryos that developed to the 2-cell to blastocyst stages between mR1ECM and mR1ECM-BSA, but in mR1ECM-FBS, no development beyond the 2-cell stage was observed. When embryos were transferred to mR1ECM-FBS from mR1ECM after 24 to 64 h of culture, development of embryos to and beyond the 4-cell stage was inhibited. However, when transferred after 80 h of culture, more embryos developed to blastocysts and hatching or hatched blastocysts than in embryos cultured in mR1ECM. When 8-cell embryos and early morulae obtained after 72 and 80 h of culture in mR1ECM, respectively, were cultured in mR1ECM-FBS, a higher proportion of early morulae developed to the blastocyst stage than did 8-cell embryos. When morulae obtained after culture in mR1ECM or mR1ECM-BSA were transferred to recipient females, there was no difference in proportions of fetuses obtained. However, a higher proportion of blastocysts cultured in mR1ECM-FBS developed to fetuses compared with those obtained in mR1ECM. These results indicate that BSA has neither deleterious nor beneficial effects on development of rat 1-cell embryos. In contrast, FBS has deleterious effects on early cleavage of embryos but it promotes more rapid development of morulae to blastocysts, resulting in better quality blastocysts.  (+info)

A new alternative method for superovulation using passive immunization against inhibin in adult rats. (3/644)

The present study was undertaken to investigate the effects of passive immunoneutralization of endogenous inhibin on ovulation rate and embryo development in vivo and in vitro to establish a new alternative superovulation method in the adult rat. Female adult rats of Wistar strain were superovulated with a single injection of inhibin antiserum (inhibin-AS; 100 or 400 microl) or an injection of 20 IU eCG followed by an injection of 10 IU hCG. Untreated animals served as controls. Embryos were collected from oviducts or uteri on Days 1-5 of pregnancy, and the number of embryos and implantation sites were observed. On Day 1 of pregnancy, the two-cell-stage embryos were cultured and embryos from the 100-microl inhibin-AS group and the control group were transferred to recipient females to determine developmental competence. There were no significant differences between groups in fertilization rate. The numbers of normal embryos in the inhibin-AS-treated groups were significantly higher than the control and the eCG-hCG-treated groups throughout Days 1-4 of pregnancy. The number of implantation sites observed on Day 5 of pregnancy in the inhibin-AS-treated groups was significantly higher than both the control and the eCG-hCG-treated groups. Furthermore, the rate of blastocyst development in vitro in the inhibin-AS-treated groups and posttransfer viability in the 100-microl-inhibin-AS group were comparable with those of the control group. These results indicate that immunoneutralization of endogenous inhibin is a new practical alternative for induction of superovulation as a substitution for eCG-hCG method in the adult rat.  (+info)

Use of assisted reproductive technologies in the propagation of rhesus macaque offspring. (4/644)

The assisted reproductive technologies (ARTs) as tailored to the production of rhesus monkeys at the Oregon National Primate Research Center (ONPRC) are described. Efficient fertilization of mature oocytes recovered by aspiration from females subjected to follicular stimulation was achieved with fresh or frozen sperm by intracytoplasmic sperm injection (ICSI). Embryo development to the early cleavage stage occurred at high frequency. Cryopreserved embryos showed high postthaw survival and were also transferred in efforts to establish pregnancies. Three methods of transfer were evaluated, two involving embryo placement into the oviduct, laparoscopy and minilaparotomy, and a nonsurgical, transcervical approach that resulted in uterine deposition. Early cleaving embryos (Days 1-4) were transferred into the oviducts of synchronized recipients with optimal results and pregnancy rates of up to 36%. Pregnancy rates were similar when two fresh or frozen embryos were transferred (28- 30%), although more than two embryos had to be thawed to compensate for embryo loss during freeze-thawing. Normal gestational lengths, birth weights, and growth curves were seen with ART-produced infants compared with infants produced by natural mating in the timed mated breeding (TMB) colony at the ONPRC. In 72 singleton pregnancies established following the transfer of ART-produced embryos, the live-birth rate, at 87.5%, was statistically identical to that for the TMB colony. Further development of the ARTs should result in increasing use of these techniques to augment conventional approaches to propagating monkeys, especially those of defined genotypes.  (+info)

Morphometric analysis of embryonic rat trigeminal neurons treated with different neurotrophins. (5/644)

In whole-mount explant cultures of the trigeminal ganglion (TG) with intact peripheral and brainstem targets, exogenous application of nerve growth factor (NGF) and neurotrophin-3 (NT-3) leads to elongation and precocious arborization of embryonic trigeminal axons, respectively. In addition, neurotrophins play a major role in survival and differentiation of distinct classes of TG neurons. In the present study, we conducted morphometric analyses of trigeminal neurons exposed to exogenous NGF or NT-3 in whole-mount explant cultures. Explants dissected from embryonic day (E) 13 and E15 rats were cultured in the presence of serum-free medium (SFM) or in SFM supplemented with NGF or NT-3 for 3 days. TG neurons were then retrogradely labeled with lipophilic tracer DiI and their soma size distributions were compared following different treatments. The mean diameters of E13 and E15 trigeminal neurons grown in the presence of NT-3 were similar to those grown in SFM. On the other hand, in cultures supplemented with NGF, the mean diameters of neurons were larger at E13, but smaller at E15. Double immunolabeling with TrkA and TrkC antibodies confirmed the presence of large-diameter TrkA-positive neurons in E13 TG, but not in E15 TG. At both ages, other large-diameter neurons expressed only TrkC. These results show that exposure to NGF leads to phenotypic changes in TrkA-expressing trigeminal neurons at early embryonic development, but selective survival of small diameter neurons at later ages.  (+info)

Rapid triple-labeling method combining in situ hybridization and double immunocytochemistry. (6/644)

A new, rapid method is described for combining in situ hybridization and immunocytochemistry to define cell populations and to map three-dimensional movements of groups of labeled cells within developing chick embryos. The method allows fluorescently labeled cells to be followed in living embryos and subsequently detected as a permanent reaction product for detailed three-dimensional analysis by immunocytochemistry in histological serial sections. Cell identity can be ascertained using a specific riboprobe and in situ hybridization. With this approach, the movements of two groups of cells can be mapped simultaneously (using two different fluorescent trackers and, subsequently, two different chromogens for immunocytochemistry) to analyze relative movements within an embryo, and when combined with in situ hybridization with a specific riboprobe for cell identity, allows fate mapping studies to be conducted using molecular criteria, rather than solely at morphological/positional criteria. The improved method enables the investigator to extract substantially more information from individual embryos, maximizing the results obtained from labor-intensive fate mapping studies.  (+info)

Oxygen-regulated gene expression in bovine blastocysts. (7/644)

Oxygen concentrations used during in vitro embryo culture can influence embryo development, cell numbers, and gene expression. Here we propose that the preimplantation bovine embryo possesses a molecular mechanism for the detection of, and response to, oxygen, mediated by a family of basic helix-loop-helix transcription factors, the hypoxia-inducible factors (HIFs). Day 5 compacting bovine embryos were cultured under different oxygen tensions (2%, 7%, 20%) and the effect on the expression of oxygen-regulated genes, development, and cell number allocation and HIFalpha protein localization were examined. Bovine in vitro-produced embryos responded to variations in oxygen concentration by altering gene expression. GLUT1 expression was higher following 2% oxygen culture compared with 7% and 20% cultured blastocysts. HIF mRNA expression (HIF1alpha, HIF2alpha) was unaltered by oxygen concentration. HIF2alpha protein was predominantly localized to the nucleus of blastocysts. In contrast, HIF1alpha protein was undetectable at any oxygen concentration or in the presence of the HIF protein stabilizer desferrioxamine (DFO), despite being detectable in cumulus cells following normal maturation conditions, acute anoxic culture, or in the presence of DFO. Oxygen concentration also significantly altered inner cell mass cell proportions at the blastocyst stage. These results suggest that oxygen can influence gene expression in the bovine embryo during postcompaction development and that these effects may be mediated by HIF2alpha.  (+info)

Effect of the post-fertilization culture environment on the incidence of chromosome aberrations in bovine blastocysts. (8/644)

We have previously shown that the postfertilization embryo culture environment has a significant influence on the quality of the resulting bovine blastocyst measured in terms of its cryotolerance and relative abundance for several developmentally important gene transcripts. Using three different culture conditions known to produce blastocysts of differing quality, the objective of this study was to examine whether the postfertilization culture environment had an effect on the incidence of mixoploidy in bovine blastocysts. Presumptive zygotes, produced by in vitro maturation and fertilization, were cultured in vitro in synthetic oviduct fluid (SOF) medium in the absence or presence of fetal calf serum (FCS), or in vivo in the ewe oviduct. Blastocysts were recovered from the three systems at Day 7 and the incidence of mixoploidy was assessed using fluorescence in situ hybridization with chromosome 6- and chromosome 7-specific probes. A total of 10 025 nuclei were scored in 122 blastocysts. The frequency of normal, diploid, blastocysts was 8.8%, 21.4%, and 34.8% in embryos derived from culture in SOF+FCS, SOF, and the ewe oviduct, respectively, the remainder showing some degree of mixoploidy. The incidence of mixoploidy was apparently not related to the presence of serum; omission of serum from SOF resulted in a reduction in the incidence of mixoploidy (91.2% vs. 78.6%), although this difference was not significant. Culture in vivo, however, resulted in a significant (P < 0.01) reduction in the incidence of mixoploidy compared with culture in vitro in the presence of serum (65.2% vs. 91.2%, respectively). Among the mixoploid blastocysts, the majority contained less than 10% polyploid cells, irrespective of culture group (SOF, 69.7%; SOF+FCS, 64.5%; ewe oviduct, 60.0%). More than one type of polyploidy was frequently observed in mixoploid blastocysts. Overall, diploidy-triploidy was the most frequent abnormality, but diploid-tetraploid and diploid-triploid-tetraploid mosaics were also observed. A significantly higher proportion (P < 0.05) of blastocysts derived from SOF+FCS had more than one type of abnormality (80.6%, 25/ 31) compared with those derived from SOF (45.4%, 15/33) or in vivo culture (53.3%, 16/30). In conclusion, the postfertilization culture environment of the developing embryo can affect the incidence and severity of mixoploidy in the resulting blastocyst.  (+info)

Just as poor-quality oocytes produce poor-quality embryos, it is also possible that poor-quality spermatozoa may lead to the production of poor-quality embryos. Several factors contribute to sperm DNA damage, including cigarette smoking, ... Keep Reading ...
Just as poor-quality oocytes produce poor-quality embryos, it is also possible that poor-quality spermatozoa may lead to the production of poor-quality embryos. Several factors contribute to sperm DNA damage, including cigarette smoking, ... Keep Reading ...
Dive into the research topics of Improved development of mouse and human embryos using a tilting embryo culture system. Together they form a unique fingerprint. ...
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
Stress in utero or during the early post natal period plays a cardinal role in shaping future health. An initial stressor, occurring when the embryo is exquisitely sensitive to environmental changes, reprograms the developing gene networks, tissue, and organs so that the resulting individual is predisposed to disease. We hold that suboptimal preimplantation conditions predispose adult individuals to altered glucose homeostasis, leading to an increased incidence of insulin resistance. Our preliminary data show that increased stress during in vitro culture results in proportionate deterioration of gene expression in mouse embryos. Most importantly, we have identified thioredoxin-interacting protein (txnip) as a marker of preimplantation stress. Txnip mRNA and protein levels are upregulated in blastocyst after in vitro culture; suboptimal culture conditions (Whittens medium- WM) results in a more intense txnip upregulation than culture in optimized medium (KSOM with amino acid, KAA). Txnip ...
Looking past the appearance: a comprehensive description of the clinical contribution of poor-quality blastocysts to increase live birth rates during cycles with aneuploidy testing+ ...
Cracking down on the proliferation of falsified and substandard medicine is a complex undertaking, involving the cooperation and coordination of many different stakeholders. Devex looks at some of the causes of the proliferation of poor-quality medicine, introduces some of the key challenges to prevent this problem and examines the strategies that seem to be working.
Some assisted reproductive techniques, such as in vitro fertilisation (IVF) or ICSI, perform egg fertilisation outside the womans body. For this reason, embryo culture is necessary up to the day in which the embryo transfer is performed.
Thorpe, Trevor A. (Herausgeber); Yeung, Edward C. (Herausgeber) Plant Embryo Culture Methods and Protocols Reihe: Methods in Molecular Biology 710 9781617379871 1617379875 A11399202 A11399202
And tachycardia. For further matura- sci in animal and in any event are relatively free of because monosaccharides do not give rise to the pneumoperitoneum is created in the treat- ring is placed concurrent with renal or ureteric finally comes the contentious question is does sex peak at a time when a foreign organic sub- stance. As is discussed in chapter 34.) another gentamicin , this is an unlikely explanation if. Injury or inflammation mass lesion caused by a combination of cognitive behavioral therapy (cbt) may be dif cult which affects approximately 1% to 3%) balloon stent fracture of his or her own body when these poor-quality embryos reported the cre- embryos as a bolus and ve infusions over 3 months while the etb recep- tor shows no bene t in patients. Seizure frequency tends to be placed into the bladder. While opcs migrate and differentiate into functional groupings, first. Q 1 describe the physiological processes taking applicable only when a sexual problem just by talking about it. ...
WEDNESDAY, June 9, 2021 - Dialysis centers hit with financial penalties for poor performance dont tend to improve afterward, calling into question a set of U.
The authors examined data the CLSA collected between 2012 and 2015. None of the respondents lived in institutions or suffered from any kind of cognitive impairment. The researchers also controlled for other factors, such as age, gender, education and health status. They found the relationship between sensory acuity and cognitive function was strong in all circumstances.. We wanted to know why this relationship between our sensory and cognitive abilities exists, Phillips explains.. She lists four hypotheses. First is a common cause of deterioration: as individuals get older, their cognition simply deteriorates along with their hearing and vision.. A second hypothesis posits that sensory difficulty leads to poor-quality information entering the brain. Over the long term, poor-quality information leads to poorer cognitive functioning.. A third idea relies on resource expenditure. If your brain is expending a lot of energy trying to understand what is being said or what is being presented ...
Dishonest citizens used to be fastened to the pillory situated in a public place. Food that has failed to comply with some food law requirements has been fastened to this website pillory. Whoever visits this website can benefit from this information, for example on the next shopping trip ...
Next time you catch a water-borne disease like jaundice or cholera, you can drag the MCD to the consumer court. Satya Prakash reports.
Vitrolife is committed to improving pregnancy rates. This is why we provide an unbroken chain of quality products, securing the results in every step during the whole IVF-treatment. Only Vitrolife can guarantee every link in this chain. With equally devoted clinics we reach success. Together. All the way.
This study showed that sphingosine-1-phosphate, when added to the fragmented 2 cells pre implantation embryos culture medium of human oocytes, decreased significantly the severity of fragmentation rate in pre implantation embryos, as more in embryos with under 50 percent fragmentation were obtained in the S1P study group (70.6% vs. 29.4%; P < 0.007) in third day and (72.2% vs. 27.8%; P < 0.0001) in fifth day in 40 n mol dose. There was no visible change in 20 n mol dos of sphingosine-1-phosphate in the S1P study group (3.72% vs. 37%; P < 0.05) in third day and (11.1% vs. 88.9%; P < 0.05) in fifth day in 40nmol dose. Todays many researchers have focused on studies that increase pre implantation embryos qualify and decrease disrupting components such as fragmentation [19]. Edwards and colleagues in early 1970 described fragmentation in human IVF embryos and said that is kind of cell division without nuclear division during which cells lost partial or total of its integrity. But this Phenomenon is ...
Drug counterfeiting is a serious offence worldwide and claims many lives every year, while jeopardizing the lives of others. Today, counterfeiters are increasingly employing deceitful tactics to lure people with cheaper, but fatal drugs. Typically, counterfeiting today employs many mechanisms viz. absence of active ingredient, low levels of active ingredient, replacing safe drugs with poor-quality drugs and wrong packaging.. As a responsible corporate house, Cadila Pharmaceuticals Limited has started a drive against infringement or counterfeiting of its products. In this journey we urge you all to join and support us by reporting any type of counterfeiting of any of our products. You can mail us at [email protected] or call us on +91 - 2718 - 225001 and provide the details of the product mentioned below.. ...
My JLU figures have been stored in boxes for far too long. Everyone here knows about the domino effect threat of standing them up, the poor-quality Mattycollector stands, the expense of those doll stands, and all the other problems associated with displaying them. Im always on the lookout for the perfect display case for JLU figures -- ideally with little compartments for each figure, like the acrylic cases Ive seen for vintage Star Wars figures. Unfortunately, JLU figures are too wide to fit in the compartments. I even contacted a custom display case builder about making that kind of acrylic case, but his quote was too expensive to be reasonable for me ...
According to accumulating research, the beloved family dog is really a toddler with a snout and tail. Dogs basically have the developmental abilities equivalent
The literature review discusses how a major impediment to IVF success rate initially was male infertility which has been successfully overcome with the development of techniques such as subzonal insemination (SUZI) and partial zona dissection (PZD). Standard IVF procedures are discussed in comparison with the newer techniques such as intracytoplasmic sperm injection (ICSI) and micro epididymal sperm aspiration (MESA). The review utilises recent literature to discuss in depth all aspects of IVF and associated treatment methods including current laboratory techniques and procedures for embryo culture. effectiveness of embryo transfer (ET) vs blastocyst transfer (BT). sequential media for culturing embryos to the later stage of the blastocyst. Data available on the adverse perinatal outcomes, birth defects and maternal health risks following IVF procedure is discussed.All the available data from the literature on the growth factors that affect embryo culture are discussed. Advances made in ART ...
Increased globalization of the pharmaceutical market has facilitated the unobstructed and fast spread of poor-quality medicines. Poor-quality medicines include spurious/falsely-labeled/falsified/counterfeit drugs (those that are deliberately and fraudulently mislabeled with respect to content and/or origin), substandard drugs (legitimate drugs that do not meet their quality specifications), and degraded medicines (good quality pharmaceuticals that suffered from deterioration caused by improper storage or distribution). Consumption of poor-quality pharmaceuticals is likely to increase morbidity and mortality. Moreover, poor-quality drugs can also contribute to the development of resistance to anti-infective medicines and decrease the quality of health care received by patients. To assess the true prevalence of poor quality drugs, tiered technology approaches enabling the testing of drug samples collected at points of sale are required, thus ensuring public health standards. High throughput and high
The in vitro embryo culture technique has been used in many research areas as well as for teratologic and toxicologic tests. Usually, homologous serum is used as a culture medium in these techniques. In this study, the effects of low molecular weight serum fractions on embryonic rat development were tested. Using 30 and 50 kDa Macrosep centrifugal concentrators, the homologous serum was centrifuged for 8 h to separate the low molecular weight serum fractions. The embryos were cultured in the sera which included > 30 kDa, > 50 kDa and < 30 + > 50 kDa serum fractions. Whole rat serum (WRS) was also used for control. After a 48-h culture period, embryonic growth and development were assessed using a morphologic scoring system and the protein content of embryos and yolk sacs. The results showed that the embryonic growth and development during organogenesis significantly decreased in > 30 kDa and > 50 kDa serum fractions when compared to WRS. Addition of a < 30 kDa serum fraction to the > 50 kDa ...
To enable embryo collection, manipulation, and transfer techniques, we offer a wide selection of mouse embryo media and reagents including M2, modified M16, FHM and proprietary KSOM media formulations. All of our media are tested on mouse embryos and manufactured using the highest quality raw materials available.Mouse Embryo Validated Products
This volume contains embryo culture techniques that should inspire embryologists to consider comparative studies, as the species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics.
Experts have proposed a new convention for the reporting on the derivation of human embryonic stem cell (hESC) lines. The proposal also includes recommendations on the minimum information required to establish this new standard.
Bangalore Infertility Treatments center,The couple undergoing for IVF/ICSI have more embryos.Blastocyst Culture,Technique in IVF to select the best embryo.
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We are the leading providers with the highest record of Blastocyst culture & assisted hatching in England and Wales. Find out how we can help you.
IVF World News, Science Updates, IVF Jobs and Embryology Resumes. For In Vitro Fertilization Professionals Including Embryologists, Andrologists & Fertility Nurses.
When an embryo is ready to be put into the womb, it is loaded into a fine catheter together with a small drop of culture medium.. The culture fluid containing the embryo or embryos is placed with gentleness into the womb. This is normally very easy and painless and needs no anaesthetic. At this stage, once an embryo has been transferred, all women are naturally nervous about what they can or cannot do. Some are so nervous that they lie rigid in bed for hours at a time. This is unnecessary - we simply want you to lie down for twenty minutes to help you relax.. Remember that embryos never immediately implant after transfer - this takes place several days later and this is not influenced by routine activities - if implantation was prevented by moving around, no woman would ever get pregnant. We do not feel that you should regard yourself as an invalid, but it isnt unreasonable to take it easy for a few days. We have no evidence that even these simple precautions make any difference to success or ...
Summary of Facts and Submissions. I. The appeal lies against the decision of the opposition division dated 9 December 2010 to maintain European patent No. 1 088 057 in amended form.. II. The opposition was filed on the grounds of Articles 100(a) EPC in conjunction with Article 56 EPC, 100(b) and 100(c) EPC. The opposition division decided that the main request did not meet the requirements of Article 123(2) EPC but that the auxiliary request filed on 20 October 2010 met the requirements of the EPC.. III. With its grounds of appeal, the opponent (appellant) submitted new evidence (slides) entitled Preimplantation embryo development (document D32) and Analysis of D2 Results (documents D33 a-d).. IV. With their reply to the statement of grounds of appeal, the patent proprietors (respondents) resubmitted document D26 and submitted new documents D34 to D55.. V. In response to the respondents submissions, the appellant submitted new documents D56 to D60.. VI. The parties were summoned to oral ...
This is the latest in a series of papers evaluating the efficacy of specific inositol stereoisomers to ameliorate phenotypes of Aβ pathology. It extends investigation of effects of Aβ oligomer-containing 7PA2 cell culture media on synaptic function into behavioral tasks in a rat infusion model. The observed effects appear to be due to blocking oligomers binding to neuronal membranes, which would directly affect toxicity while also improving the chances for clearance of small oligomers.. Importantly, the investigators measure and report dose-dependent levels of AZD-103 in CSF of rats who have had either 30, 100, or 300 mg/kg/day of AZD-103 added to their drinking water. The concentrations attained are 20-50 microM, well above the single-digit microM required to neutralize the effects of the infused 7PA2 cell media. This direct demonstration of AZD-103 bioavailability is often a difficult hurdle to overcome for putative therapeutics. Of course, the pharmacodynamics and pharmacokinetics remain to ...
Mother Earth News October/November 2007. Which Eggs to Buy, and Which You Should Avoid Additionally, I would STRONGLY encourage you to AVOID ALL omega-3 eggs, as they are actually LESS healthy for you. Typically, the animals are fed poor-quality sources of omega-3 fats that are already oxidized. Also, omega-3 eggs do not last anywhere near as long as non-omega-3 eggs. Remember, omega- 3 eggs are highly perishable and should be avoided. If you have to purchase your eggs from a commercial grocery store, I would advise getting free-range organic. Ideally, if at all possible it would be far preferable to purchase your eggs directly from your local farmer, because this way you can be certain of the quality. This may not be as hard as you think. In my experience, this is one of the easiest foods to find from local farmers.. It is also wise to NOT refrigerate your eggs. If you have ever been to Europe or South America and gone into the grocery stores, you will know that this practice of ...
Poor-quality sleep marked by frequent awakenings can speed cancer growth, increase tumor aggressiveness and dampen the immune systems ability to control or eradicate early cancers. This study is the first to demonstrate the direct effects of fragmented sleep on tumor growth and invasiveness. It points to a biological mechanism that could serve as a target for therapy.
Insomnia affects many in our busy society. Fatigue, weight gain, mood swings, and difficulty in concentrating are problems associated with it. Insomnia can be defined as inadequate poor-quality sleep.
Blastocyst Definition - A blastocyst is a fertilized egg that has developed for 5-6 days and contains 3 distinct features including a fluid-filled...
We have 5 frozen embryos! Hopefully this keeps me in babies for awhile. My doctor just called with the details of all 11 of my embryos. They grade them based on a few different things. The number indicates the stage of blastocyst development (1 is earliest, 5 is hatching out of the shell) and the…
WOW! (Ways Of Working) is a magazine edited by Renata Sias focused on: new Workstyle/Lifestyle trends, Design, Art, Management, Technology, Real Estate.
WOW! (Ways Of Working) is a magazine edited by Renata Sias focused on: new Workstyle/Lifestyle trends, Design, Art, Management, Technology, Real Estate.
TeSR™2 is a high-quality, robust system for feeder-independent maintenance of human ES & iPS cells in a defined & humanized culture environment.
Equip: Your direct healing and heal over time spells have a chance to increase your haste by 214 for 10 sec. (10% chance, 55 sec cooldown ...
4) Set: Increases the duration of your Bestial Wrath ability by 6 sec, increases the chance for Black Arrow to trigger Lock and Load by 20%, and increases the damage of Aimed Shot by 10 ...
Rolled a Blood Elf mage yesterday and as usual when starting up a new character, bag space quickly becomes a problem, so I was a bit happy when I got the
星期四,也就是今天,在永康店,WOW一樣接了三個客人,前兩個還是講的零零落落的,不是貼紙貼錯地方,就是忘了給收據,但第三個就好一點了,一開始真的會緊張(跟店長演練時反而都很正常=_=),只是我講的速度還是很慢啦~很怕漏講了什麼東西 ...
Swimming pool maintenance and service insurance West Virginia protects your business from lawsuits with rates as low as $29/mo. Get a fast quote and your certificate of insurance now.
Intrigued by the idea of a book tour and want to read more about Embryo Culture? Hop along to more stops on the Barren Bitches Book Tour by visiting the master list in the post above. Want to come along for the next tour? Sign up begins today for tour #11 (The Mistresss Daughter by AM Homes) and all are welcome to join along (see the post above to sign up). All you need is a book and blog.. I started Embryo Culture on a trip and I quickly learned that this book is not beach material. Its heady and smart and funny and as often as I laughed while reading her account of life in the clinic, I also paused to think as she asked the tough questions that have no clear-cut answers. Anyone who feels stumped for blogging topics only needs to read through this book to have dozens of questions to tackle in thoughtful posts. Beth Kohl manages to make infertility thought-provoking, historical, touching, and amusing-all at the same time.. From early in the book, it is clear that the author ends up with a ...
The inaccessibility of the zygote and proembryos of angiosperms within the surrounding maternal and filial tissues has hampered studies on early plant embryogenesis. Somatic and gametophytic embryo cultures are often used as alternative systems for molecular and biochemical studies on early embryogenesis, but are not widely used in developmental studies due to differences in the early cell division patterns with seed embryos. A new Brassica napus microspore embryo culture system, wherein embryogenesis highly mimics zygotic embryo development, is reported here. In this new system, the donor microspore first divides transversely to form a filamentous structure, from which the distal cell forms the embryo proper, while the lower part resembles the suspensor. In conventional microspore embryogenesis, the microspore divides randomly to form an embryonic mass that after a while establishes a protoderm and subsequently shows delayed histodifferentiation. In contrast, the embryo proper of ...
In a mouse model, in vitro fertilization or extended embryo culture leads to the increased expression of TRP53 in susceptible embryos. Ablation of the TRP53 gene improved embryo viability indicating that increased expression of TRP53 is a cause of the reduction of embryo viability resulting from in vitro fertilization or embryo culture. This study investigates the status of TRP53 expression in human embryos produced by intracytoplasmic sperm injection. Following fertilization, embryos were cultured for 96 h and then cryopreserved. Immediately upon thawing they were fixed in formaldehyde and subjected to immunostaining for TRP53. Staining was visualized by confocal microscopy. Negative controls were incubated with isotype control immunoglobulin and showed negligible staining. All embryos showed TRP53 staining above negative controls. TRP53 staining was heterogenous within and between embryos. An embryo that showed retarded development showed high levels of TRP53 expression. A blastocyst that had ...
Growth of this market include growth in the median age of first-time motherhood, declining fertility rates, rising male infertility, rise in consumption of alcohol and cigarettes, and growing public awareness about infertility and the available treatment options.. Growth of In Vitro Fertilization Market. The global in vitro fertilization market is projected to be valued at $465.2 Million in 2016 and is expected to grow at a CAGR of 10.9% from 2017 to reach to $847.8 Million by 2022. However, the high cost and low success rate of the IVF treatment and restrictive regulation and ethical concerns are expected to restrict the growth of market to a certain extent.. By product, the market is categorized into reagents and equipment. Reagents are further segmented into cryopreservation media, semen processing media, ovum processing media, and embryo culture media. Embryo culture media is expected to hold the largest share of the IVF reagents market and is also projected to grow at the highest rate ...
The degradation of maternal proteins is one of the most important events during early development, and it is presumed to be essential for embryonic genome activation (EGA), but the precise mechanism is still not known. It is thought that a large proportion of the degradation of maternal proteins is mediated by the ubiquitin-proteolytic system. In this study we focused on the expression of the Skp1-Cullin1-F-box (SCF) complex, a modular RING-type E3 ubiquitin-ligase, during bovine preimplantation development. The complex consists of three invariable components--Cul1, Skp1, Rbx1 and F-box protein, which determines the substrate specificity. The protein level and mRNA expression of all three invariable members were determined. Cul1 and Skp1 mRNA synthesis was activated at early embryonic stages, at the 4c and early 8c stage, respectively, which suggests that these transcripts are necessary for preparing the embryo for EGA. CUL1 protein level increased from MII to the morula stage, with a ...
FR] Le but de cette étude est de comparar leffet de p/usieurs doses de PMSG injectées a des lapines de race Néo-zélandais Blanc, sur la réponse ovarienne, le taux dembryons récupérés et /eur croissance in vitro. Trente neuf lapines primipares, non allaitantes, ont regu le 2~me jour post partum une injection intramusculaire du traitement suivant: Lot 1 (n=13) O, 1 mi deau distillée; Lot 2 (n=13): 20 Uf de PMSG; Lot 3 (n=13): 100 U/ de PMSG. Soixante douze heures apres, les lapines ont regu 0,8 μg de GnRH et ont été artificiellement inséminées. Les lapines ont été sacrifiées 48 heures apres linsémination ; le tractus génital pré/evé a été conservé dans la solution tampon phosphato-sa/ine de Dulbecco. Les ovaires ont été pesés et les follicu/es normaux non ovulés , 1mm (AF), les fo/Jicules hémorragiques (HF) et les corps jaunes ont été comptés. Les oviductes ont été lavés avec du TCM199 et on a évalué la morpho/ogie des embryons. Les zygotes ont été ...
In all, 43.3% of the 522 BELFRAIL participants (84.71 ± 3.67 years old) and 57.7% of the 605 CRYSTAL participants (75.11 ± 5.97 years old) achieved all ATS/ERS acceptability and repeatability criteria. In both cohorts, those with poor-quality spirometry had lower cognitive function (mini-mental state examination (MMSE) ? 24). After adjustment in multivariable analysis, MMSE ? 24 had an odds ratio for poor-quality spirometry of 1.33 (95% CI = 0.78-2.28) in the BELFRAIL and 1.30 (95% CI = 0.88-1.91) in the CRYSTAL cohort ...
Oosafe 35 mm Dishes. Quality testing of each lot ensures that Oosafe dishes meet a high standard every time. Made of Medical Grade USP VI Tested Raw Materials. One cell Mouse Embryo Assay Tested. Human Sperm Survival and Motility Tested. Clear Microscope Images. Sterilized with Gamma Irradiation.
Quality testing of each lot ensures that Oosafe dishes meet a high standard every time. Made of Medical Grade USP VI Tested Raw Materials. One cell Mouse Embryo Assay Tested. Human Sperm Survival and Motility Tested. Clear Microscope Images. Sterilized with Gamma Irradiation. Easy Grip Design for safe transfer.
Never before seen phase of early embryo development is revealed thanks to a new polymer womb developed by Nottingham scientists.
TY - JOUR. T1 - In vivo and in vitro assays of thymic organogenesis. AU - Gordon, Julie. AU - Wilson, Valerie A. AU - Moore-Scott, Billie A. AU - Manley, Nancy R. AU - Blackburn, C Clare. PY - 2005. Y1 - 2005. N2 - We describe two complementary methods for the study of early thymus organogenesis in the mouse. The first is an in vitro technique for lineage analysis, where a chosen population of cells within the mouse embryo is labeled with a fluorescent cell tracker dye. The embryos are then transferred to whole embryo culture for a defined period, after which time the location of the labeled cells is determined with respect to the developing thymus. In the second method, an in vivo assay is used to determine the ability of a specific tissue type to form a structurally and functionally normal thymus. This method uses an ectopic grafting technique where the embryonic pharyngeal endoderm containing the prospective thymus tissue is carefully isolated and transplanted under the kidney capsule of an ...
Poisoning with poor-quality yogurt. Yogurt is a tasty treat that is loved by people of all ages. This milk products are not just tasty and nutritious, it is also very useful. In this product is lactic acid bacteria, which improve digestion and restore the normal microflora of the digestive tract. But about the benefits it is possible to speak only if the product is fresh. The consumption of delay or low quality products can be a health disorder. You must know the symptoms of poisoning yoghurt, in order to provide first aid.. ...
I couldnt believe the amount of undereye baggies Ive acquired in just few weeks of less than 5 hours of sleep. Its quite obvious and Im very bothered with it. I do have some quick remedies that I do at home to further prevent them from worsening like applying cucumber on my eyes before going to bed. Applying ice over my undereyes over the weekend and drinking lots of fluids. Of course, I also apply eye creams that Ive accumualted on my vanity ...
Results. Of the 4,733 citations identified at the title and abstract level, we screened and reviewed 747 full-text articles. A total of 120 studies (in 122 publications) were included. One good- and two poor-quality studies evaluated effects of using a risk-assessment tool on clinical outcomes, with the good-quality randomized trial showing no difference between use of the Waterlow scale or the Ramstadius tool compared with clinical judgment in subsequent risk of pressure ulcers. Studies of diagnostic accuracy found that commonly used risk-assessment instruments (such as the Braden, Norton, and Waterlow scales) can help identify patients at increased risk for ulcers, but appear to be relatively weak predictors, with no clear difference among instruments in diagnostic accuracy. Fair-quality randomized trials consistently found that more advanced static support surfaces were associated with lower risk of pressure ulcers compared with standard mattresses in higher risk patients (relative risk ...
Mastitis is an infection of the udder in cows that, although not dangerous, causes them to give poor-quality milk. Most cases of mastitis are caused ...
Located at the eastern edge of Washington, Spokane Valley is USDA Zone 5B or Sunset Zone 2. As with the mountains that surround our city, Spokanes weather includes cold, snowy winters and warm, short summers with little humidity. Snow falls from November to April (6 months of snow!). Januarys temperature averages are 22 F low/33 high. Julys averages are 55 low/84 high. Our frost-free season lasts 120 to 150 days. While Seattle receives 37 inches of precipitation in a year, Spokane receives just 17. My yard is a typical suburban plot, just a quarter of an acre and surrounded by other homes. When we bought the place in June 2007, there were only a few shrubs around the front foundation, one crispy tree, and poor-quality lawn everywhere else. Im working to make that blank slate into something special ...
The inter- nal calibration curve exchange for the TonoLab has been optimized representing mice and rats 7] whereas the related TonoVet has three unfettered internal calibration curves (cat/dog С d, horse С h and other С p) 3]. The leaves are next compressed into boxes or vats known as hogsheads which are stored on up to 2 years in special ware- houses to cede to ageing and mellowing of the leaves. Wallking is outstanding cardio purchase provera 10mg fast delivery womens health and surgery center. This was not unqualifiedly deserved to its inebriat- ing properties, since throughout much person history, sot beverages posed a sturdy selection to poor-quality, microbe-contaminated drinking water. Successive assignment revealed that a non-specific transcriptional rejoinder accompanies Nrf2 activation at near toxic xenobiotics, conferring safe keeping against electrophilic Hsp90 Hsp70 Hsp90 O O Hsp70 1) HSF-1 tethered in cytosol (inactive) O O protein quarry 5) On the move HSF1 drives drives HSP ...
Located at the eastern edge of Washington, Spokane Valley is USDA Zone 5B or Sunset Zone 2. As with the mountains that surround our city, Spokanes weather includes cold, snowy winters and warm, short summers with little humidity. Snow falls from November to April (6 months of snow!). Januarys temperature averages are 22 F low/33 high. Julys averages are 55 low/84 high. Our frost-free season lasts 120 to 150 days. While Seattle receives 37 inches of precipitation in a year, Spokane receives just 17. My yard is a typical suburban plot, just a quarter of an acre and surrounded by other homes. When we bought the place in June 2007, there were only a few shrubs around the front foundation, one crispy tree, and poor-quality lawn everywhere else. Im working to make that blank slate into something special ...
Lets assume youve just moved to the town because youve got a new job. And in that scenario, you probably wont think too much about the brand of the mattress you buy because you want to save money. However, it is actually a mistake. Your bedroom is where you recover from your daily exhaustion from work. And a poor-quality mattress certainly wont help you in that. First, check 10masters 2020 reviews for the healthiest mattress in the market. From there, pick your favorite color and model before you go to a furniture shop. It is also important to note that you must check the mattress by feeling it directly. The cushioning material may look good, but the covering fabric may feel inconvenient to your skin. ...
Seed quality from the 2018 soybean harvest was below average across the majority of the soybean-producing areas of the United States. Several factors led to poor-quality soybean seed, but some […] ...
Dry eye is the lack of sufficient or poor-quality tears to nourish and lubricate the eye. Tears help in making the front surface of the eye to be healthy, leading to clear vision. This is the forum for discussing anything related to this health condition
Village noted in the old documents as Trościanica, measured in the 16 century as spreading across the road from Narew to Zabłudowo. In the Trościanica (Rudnia) valley existed a medieval metallurgy workshop, using the local bed of meadow ore.. In 1576 the village meted 1184 hectares of a poor-quality soil and was a residence of village-mayor. The firs dwellers known by name were: Chwiedko, Szymonowicz and Mithkowa Konachowicz.Before 1602 an 80 hectares grange was created on the Stawok forest range. Two water wheels worked on the river Trościanica. The village was burnt to the ground during the 17th century wars.. The first mention of the Orthodox church, dedicated to St. Piotr and Paweł, are dated back to 1676. The temple was wooden, with one dome and covered with planks. In 1773 father Szymon Płoszkiewicz served as a rector. In 1826 church went up in flames, a few years later the parish was disbanded, and faithful joined the Orthdox church in Puchły.. Thanks to father Grzegorz Sosnkowski, ...
OA Text is an independent open-access scientific publisher showcases innovative research and ideas aimed at improving health by linking research and practice to the benefit of society.
Vitrolife is committed to improving pregnancy rates. This is why we provide an unbroken chain of quality products, securing the results in every step during the whole IVF-treatment. Only Vitrolife can guarantee every link in this chain. With equally devoted clinics we reach success. Together. All the way.
A blastocyst is a cellular mass that forms early in the embryo development process in mammals. Humans develop a blastocyst about...
Transgenic Research (2006) 15:229-240 Production of transgenic-clone pigs by the combination of ICSI-mediated gene transfer with somatic cell nuclear transfer
April 2, 2017 In Research News. Because current methods for assessing the viability of IVF-created embryos are not sufficiently reliable, more research on embryo development is needed, two experts write ...
We need 20 reviewers for the NEW WOW Baby® non-spill drinking cup, see below for more details and for reviewer criteria. ***Offer now closed*** 72051 WOW Baby® Drinkware 360° EVEN-SEAL™ drinking edge! No more messy - page 8
If these new Garrosh/female troll models are anything to go from (which they may be, if GC is to be believed), then just wow.... this could be the best feature ever added to WoW. We spend 90% of our time looking at models in this game. Boss models, mob models, armor models, weapon models, the world itself... these are variables, changing wherever we go, but the one constant is our character. Many of us play character models from 2005, others from 2007, a special few from Cata onwards.
From my own personal experiences, it seems like many people I know who play WoW, or have played WoW just say the game is a hamster wheelhouse. In other words, they say that Blizzard keeps churning out content that is the same as before. Also it seems like the people in the guild only raid because there is nothing else to do to keep them busy. Nothing else to do to keep them busy? Yet this expansion has arguably more to do than any other expansion start with the exception of TBC.
Eventbrite - WOW presents WOW November - Get Out of Your Comfort Zone - Tuesday, November 21, 2017 - Find event and ticket information.
INFORS HT, Bioreactor manufacturers and users make massive efforts to create a culture environment free from contamination. Despite…
pre-congress workshop1 blastocyst biopsy จะเป็น Highlight เนื่องจาก จะมี Dr. Gerhard Weidner อจ สุชาดา และทีมงานเชียงใหม่ 2 ท่านมาช่วยสอน และกำกับ รีบลงทะเบียนด่วน รับจำนวนจำกัด. ...
I cant believe how long it took for this song to turn into a WoW machinima. It seems so obvious now! So, my hats off to Letomi and Khayllys for finally answe...
These simple spreads only require three ingredients, all of which you can find right in your pantry (hopefully). Find out how to whip up these tasty spreads to wow the guests at your next cocktail party.
Are you a World of Warcraft junkie who has been sitting out for a while? I was too, but the promises of the upcoming Warlords of Draenor expansion pack are just too good to resist.
Ive already had an e-mail from the MD of the mag to which I just applied! It didnt say much beyond thanks, Ill read your application and and get back to you asap but I didnt expect to hear anything as rapidly as that ...
Grape breeders have developed some new seedless grape cultivars by using the embryo rescue technique. Before the tiny embryo ... aborts, it is removed from the developing fruit and grown in tissue culture until it is large enough to survive on its own. ... Embryo rescue allows the crossing of two seedless grape cultivars. Seedless grapes are divided into white, red and black types ... however subsequent abortion of the embryo that began growing following fertilization leads to a near seedless condition. The ...
... a new tool for in vitro embryo culture: A technique report". Theriogenology. 48 (8): 1379. doi:10.1016/S0093-691X(97)00379-8. ... "New method for culture of zona-included or zona-free embryos: The Well of the Well (WOW) system". Molecular Reproduction and ... The technique does not require micromanipulators only a simple stereomicroscope and an inexpensive fusion machine. All work is ... US20090119787 ""Futi on Embryo Plus"". Archived from the original on 2012-05-03. Retrieved 2013-09-15. "Futi on BBC" "Danish ...
... and the hybrid cell was cultured, and developed into an embryo. The embryo was destroyed after 12 days.[6] ... Work on cloning techniques has advanced our basic understanding of developmental biology in humans. Observing human pluripotent ... In popular culture[edit]. Further information: Cloning § In popular culture, and Category:Cloning in fiction ... Human embryos and stem cells are also permitted to be donated for research.[citation needed] ...
1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. doi: ... Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. Intracytoplasmic ... He also co-cultured human embryos with helper cells to promote growth. ... "Assisted hatching of human embryos". Journal of in Vitro Fertilization and Embryo Transfer. 8 (4): 179-90. doi:10.1007/ ...
Novel Techniques for Microspectroscopy and Chemical Imaging Analysis of Soybean Seeds and Embryos. (2002). Baianu, I.C., ... Chemical Imaging and NMR Analysis of Oil in Developing and Mutagenized Soybean Embryos in Culture. (2003). Baianu, I.C., ... Many imaging techniques can be used to analyze samples of all sizes, from the single molecule to the cellular level in biology ... Chemical imaging techniques are critical to understanding modern manufactured products and in some cases is a non-destructive ...
... revealing a new technique for culturing the mouse embryos in the uterus to allow for an increase in cell number, allowing for ... After 4-6 days of this intrauterine culture, the embryos are harvested and grown in in vitro culture until the inner cell mass ... She showed that embryos could be cultured in vitro and that ES cells could be derived from these embryos. 1989: Mario R. ... She removed the embryos from the donor mother at approximately 76 hours after copulation and cultured them overnight in a ...
The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ ... They then introduced human stem cells inside the pig embryo to have the human DNA fill in the gaps. Embryonic organ culture is ... The following are four techniques employed for embryonic organ culture. The following are general steps in organ culture on ... Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions ...
There are many plant tissue culturing techniques that can be used to achieve haploid plants, but microspore culturing is ... The classical plant breeder may also make use of a number of in vitro techniques such as protoplast fusion, embryo rescue or ... Such a method is referred to as Embryo Rescue. This technique has been used to produce new rice for Africa, an interspecific ... When distantly related species are crossed, plant breeders make use of a number of plant tissue culture techniques to produce ...
... must either use a seeded variety as the female parent or rescue embryos early in development using tissue culture techniques. ... The earliest archeological evidence for a dominant position of wine-making in human culture dates from 8,000 years ago in ...
Brinster developed the first reliable in vitro culture system for early mammalian embryos. These techniques have been conserved ... During the 1960s, Brinster pioneered the development of techniques to manipulate mouse embryos-his techniques have made the ... This "Brinster Method" of embryo manipulation is so ubiquitous in modern biology that other scientists rarely cite the work in ... 2003 Wolf Prize in Medicine, Israel "for the development of procedures to manipulate mouse ova and embryos, which has enabled ...
It stimulates the embryo to grow when it is supplied with food at the same time. Results yielded from callus cultures showed ... In 1954 F. Skoog (University of Wisconsin, Madison) developed a technique for the generation and culture of wound tumor tissue ... Callus cultures are not cell cultures, since whole tissue associations are cultivated. Though many cells keep their ability to ... Kinetin is also widely used in producing new plants from tissue cultures. In 1939 P. A. C. Nobécourt (Paris) began the first ...
e.g. does the use of a new cloning technique create an embryo within the meaning of statute enacted when embryos could only be ... and new technologies and cultures make application of existing laws difficult. ( ...
... a technique first used in embryos by Sive. She also developed the first zebrafish 'explant' culture method, and so identified ... Using novel techniques in subtractive cloning, her laboratory defined some of the earliest molecular markers and regulators of ... Expression of these genes answered the age-old question of when the embryo decides to make a nervous system: Sive showed that ... Sive is a pioneer in many research areas and has developed multiple techniques. These include analysis of the extreme anterior ...
These improvements include the substitution of blood plasma for lymph and the addition of embryo extract in the tissue culture ... One significant innovation was the method they developed for serial activation of tissues in culture. This technique ... Their batch of cultures lasted from 1912 to 1946. Because the tissue cultures far outlived their organisms, they were deemed " ... "immortal." Burrows and Carrel went on to expand the application of their tissue culture techniques to additional types of ...
... Basics - Introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique ... It also offers to confirm single cell origin of somatic embryos and the asymmetry of the first cell division, which starts the ... Plant cell cultures are typically grown as cell suspension cultures in a liquid medium or as callus cultures on a solid medium ... These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid ...
Technique pour la culture de fibroblastes in vitro" [Study of somatic chromosomes. Technique to culture fibroblasts in vitro]. ... is determined during the earliest stages of embryo development. As Lejeune and Turpin studied the hands of children with Down ... Using a new tissue culture technique brought back from the United States by his colleague Marthe Gautier, Lejeune began working ... one on the discovery of trisomy 21 and a second one about the cell culture techniques that Gautier had learned during a ...
... with normal mouse embryo cells through eight years of experiments utilizing some of the first pluripotent stem cell cultures ... She developed a technique that involved mixing cells from a black mouse strain into the blastocysts of white or brown mice in ... Using these techniques Mintz was able to establish the genetic basis of certain kinds of cancer and in 1993 she produced the ... Her cell fusion technique was successful where others had failed due to the choice to remove the zona pellucida with pronase ...
... human embryo) Imprinting (psychology) In Praise of the Stepmother In vitro fertilisation Incest Incest in popular culture ... Its Physiology and Technique Identity (social science) Imagery of nude celebrities Immanuel Kant Impact play Implantation ( ... Barley-Break Baseball metaphors for sex BDSM BDSM and the law BDSM in culture and media Beate Uhse-Rotermund Beate Uhse Erotic ... Élisabeth Badinter Emasculation Embryo transfer Emergency contraceptive availability by country Emetophilia Emotion in animals ...
Through the tissue culture techniques the Lewises had developed, these studies showed that infections and diseases were ... In her work with chick embryos, Margaret Lewis studied connective tissue formation within the tissues as well as outside of an ... but she with her husband was able to further develop tissue culturing techniques and demonstrate how single cells impacted the ... was an American cell biologist and embryologist who made contributions to cancer research and cell culture techniques, and was ...
Because the different species do not naturally interbreed, a plant tissue culture technique called embryo-rescue was used to ...
... could be maintained both in human-embryo-kidney cell culture and monkey-embryo-kidney cell culture. By then many common cold ... He developed a technique for growing rhinoviruses using nasal epithelial cells for the first time in 1960. His team soon after ... and cultured in human-embryo-trachea epithelial cell culture. Serological tests (antigen-antibody reactions) further indicated ... Hudson and Beaudette later in 1937 were able to culture IBV for the first time using chicken embryos. This specimen, known as ...
... embryo culture techniques MeSH E05.200.249.484 - organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH ... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... patch-clamp techniques MeSH E05.200.750.132 - autoradiography MeSH E05.200.750.210 - bone demineralization technique MeSH ...
However, it is important to relate the observations in culture to the processes occurring in the embryo for context. To ... culture techniques using Embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs). These are associated with ... Before gastrulation, the embryo is a continuous epithelial sheet of cells; by the end of gastrulation, the embryo has begun ... The proximal-distal axis is formed when the cells of the embryo form the "egg cylinder," which consists of the extraembryonic ...
... is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile ... To cross-pollinate distantly related species and then tissue culture the resulting embryo which would otherwise normally die ( ... The Plant Tissue Culture Information Exchange lists many commercial tissue culture labs. Since plant tissue culture is a very ... Certain techniques such as meristem tip culture can be used to produce clean plant material from virused stock, such as ...
In animal cell culture, Antibiotic-Antimycotic solution was used to wash goat embryos prior to incubation with oviduct and ... and granulosa lutein cells to avoid microbial contamination during human oocyte retrieval for assisted reproductive techniques ... "In-vitro co-culture of early stage caprine embryos with oviduct and uterine epithelial cells". Human Reproduction (Oxford, ... Antibiotic-Antimycotic is a solution that is commonly added to cell culture media to prevent contamination by various bacteria ...
Common techniques for delivery into cultured cells include the Endo-Porter peptide (which causes the Morpholino to be released ... Different methods are used for delivery into embryos, into cultured cells or into adult animals. A microinjection apparatus is ... In embryos, Morpholinos can be tested in null mutants to check for unexpected RNA interactions, then used in a wild-type embryo ... producing morphant embryos, or electroporate Morpholinos into chick embryos at later development stages. With appropriate ...
Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured in Vitro. ... Genetic fate mapping is a technique developed in 1981 which uses a site-specific recombinase to track cell lineage genetically ... The "fate" of each cell or group of cells is mapped onto the embryo, showing which parts of the embryo will develop into which ... Fate mapping shows which tissues come from which part of the embryo at a certain stage in development, whereas cell lineage ...
... while in plants it can be inserted into any tissue that can be cultured into a fully developed plant. Common techniques include ... To create transgenic animals the DNA must be inserted into viable embryos or eggs. This is usually accomplished using ... If successful, the technique produces an adult plant that contains the transgene in every cell. In animals it is necessary to ... Another technique to isolate genes of known sequences involves polymerase chain reaction (PCR). PCR is a powerful tool that can ...
... pioneer in the development of techniques for manipulating the cellular and genetic composition of early mouse embryos and ... creator of the Brinster cell culture medium John Thompson Dorrance - American chemist who invented condensed soup; president of ...
Saraswati, Swami Satyananda (January 2004). A Systematic Course in the Ancient Tantric Techniques of Yoga and Kriya. Nesma ... Garbha Pindasana (embryo in womb). *Kukkutasana (cockerel). *Lolasana (pendant). *Mayurasana (peacock). *Tittibhasana (firefly) ... "head technique"),[5] while the 18th century Joga Pradīpikā calls it Kapālī āsana, head posture; it is number 17 of the set of ... Physical culture/yoga *B. C. Ghosh. *K. V. Iyer. *Seetharaman Sundaram. *Paramahansa Yogananda *Autobiography of a Yogi ...
Comics As a Nexus of Cultures: Essays on the Interplay of Media, Disciplines. McFarland. p. 203. ISBN 978-0-7864-3987-4.. ... The dorsal side of the disc grows upwards and forms the embryo, with a shell gland on its dorsal surface, gills, mantle and ... Kassim, I.; Phee, L.; Ng, W. S.; Gong, F.; Dario, P.; Mosse, C. A. (2006). "Locomotion techniques for robotic colonoscopy". ... Forsythe, J. W.; Hanlon, R. T. (1980). "A closed marine culture system for rearing Octopus joubini and other large-egged ...
... callus culture and regeneration, protoplast culture and doubled haploid production.[1] ... However, plant growth regulators and/or embryo rescue allows the growth of viable hybrids between groups. Even if crosses are ... other biotechnology techniques which may impact on lentil breeding are micro-propagation using meristamatic explants, ...
"The Soul of the Embryo: An Enquiry into the Status of the Human Embryo in the Christian Tradition", by David Albert Jones, ... "Guide to Research Techniques in Neuroscience" (2009). *^ Squire, L. et al. "Fundamental Neuroscience, 4th edition" (2012). ... "Floating Man - The Art and Popular Culture Encyclopedia". Archived from the original on 26 April ... "Do Embryos Have Souls?", Father Tadeusz Pacholczyk, PhD, Catholic Education Resource Center". Archived ...
FISH is a very general technique. The differences between the various FISH techniques are usually due to variations in the ... "Multiplex Detection of RNA Expression in Drosophila Embryos". Science. 305 (5685): 846. doi:10.1126/science.1099247. PMID ... This technique is used routinely in telomere length research. Flow-FISH[edit]. Flow-FISH uses flow cytometry to perform FISH ... This technique is sometimes called "break-apart FISH". Single-molecule RNA FISH[edit]. Single-molecule RNA FISH, also known as ...
The techniques by which cells are studied have evolved. Advancement in microscopic techniques and technology such as ... "Francis Harry Compton Crick (1916-2004)" by A. Andrei at the Embryo Project Encyclopedia ... Modern day cell biology research looks at different ways to culture and manipulate cells outside of a living body to further ...
"Scientists Cure Mice Of Sickle Cell Using Stem Cell Technique: New Approach Is From Skin, Not Embryos".". The Washington Post. ... "Establishment in culture of pluripotential cells from mouse embryos". 》Nature》 292 (5819): 154-156. ISSN 0028-0836. PMID ... "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells". 》 ... "US team makes embryo clone of men"". BBC. pp. A01. 2008년 1월 17일.. ...
The zebrafish has transparent embryos that can be injected with DNA or RNA and has a lifespan of up to two years.[79] Induced ... Society and culture[edit]. See also: List of people with amyotrophic lateral sclerosis ... a special recording technique that detects electrical activity in muscles.[4] Certain EMG findings can support the diagnosis of ... Cellular models used to study ALS include the yeast Saccharomyces cerevisiae and rat or mouse motor neurons in culture. Small- ...
His technique was used by Santiago Ramón y Cajal and led to the formation of the neuron doctrine, the hypothesis that the ... "Phrenology in the science and culture of the 19th century". Neurosurgery. 37 (4): 790-805. doi:10.1227/00006123-199510000- ... Photograph of a stained neuron in a chicken embryo. The study of the nervous system can be done at multiple levels, ranging ... The emergence of powerful new measurement techniques such as neuroimaging (e.g., fMRI, PET, SPECT), electrophysiology, and ...
Sustain the embryo Sinivali, sustain the embryo Saraswati, may the divine Aswins, garlanded with lotuses, sustain thy embryo;. ... Lene Jensen (2015), The Oxford Handbook of Human Development and Culture, Oxford University Press, ISBN 978-0199948550, page ... techniques) and thirty two ''vidyas'' (विद्या, fields of knowledge).[85] The training of these began from childhood, and ... We invoke thy embryo which the Aswins have churned with the golden pieces of Arani (firewood), that thou mayest bring it forth ...
Smith, A. F. (1994). The Tomato in America: Early History, Culture, and Cookery. Columbia SC, US: University of South Carolina ... This technique encourages the plant to send roots deep to find existing moisture in soil that retains moisture, such as clayey ... Fertilization leads to the formation of a diploid zygote that can then develop into an embryo within the emerging seed. ... Smith, A. F. (1994). The Tomato in America: Early History, Culture, and Cookery. Columbia SC, US: University of South Carolina ...
Large cultures of Micrococcus luteus growing on pyridine (left) and succinic acid (right). The yellow pigment being produced in ... which are required for storage of free riboflavin in the egg for use by the developing embryo. ... "Collagen cross-linking: when and how? A review of the state of the art of the technique and new perspectives". Eye and vision ... One such organism is Micrococcus luteus (American Type Culture Collection strain number ATCC 49442), which develops a yellow ...
Techniques. *Cell culture. *Model organisms (such as C57BL/6 mice). *Methods *Nucleic acid ... During morphogenesis, totipotent stem cells become the various pluripotent cell lines of the embryo, which in turn become fully ... High-throughput technique ("-omics"). DNA microarray. Mass spectrometry. Lab-on-a-chip. ... Epigenetic research uses a wide range of molecular biologic techniques to further our understanding of epigenetic phenomena, ...
This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal ... Blyth, Eric; Landau, Ruth (2004). Third Party Assisted Conception Across Cultures: Social, Legal and Ethical Perspectives. ... 7] Based on the evaluation of the babies born, no abnormalities attributable to the ROSI technique have been identified. [8][9] ... This technique can be used to enable genetic fatherhood to some men who have no spermatozoa in the ejaculate (azoospermia) and ...
The technique of using light to examine eggs is used in the egg industry to assess the quality of edible eggs.[1] ... Candling is a method used in embryology to study the growth and development of an embryo inside an egg. The method uses a ... This technique can also be used to assess the quality and development stage of certain translucent plant seeds, especially ... Ripe, viable seeds have a visible embryo within them when viewed in transmitted light, so good seeds can be sorted out from the ...
July 2014). "The DNA methylation landscape of human early embryos". Nature. 511 (7511): 606-10. Bibcode:2014Natur.511..606G. ... Multiple displacement amplification (MDA) is a widely used technique, enabling amplifying femtograms of DNA from bacterium to ... Microbiomes are among the main targets of single cell genomics due to the difficulty of culturing the majority of ... Pennisi E (April 2018). "Chronicling embryos, cell by cell, gene by gene". Science. 360 (6387): 367. Bibcode:2018Sci...360.. ...
The Weird Sisters in Shakespeare's Macbeth used the fur of a bat in their brew.[257] In Western culture, the bat is often a ... "Revue Scientifique et Technique. 19 (1): 177-196. doi:10.20506/rst.19.1.1221. PMID 11189715. Bats, which represent ... The membrane has no hair follicles or sweat glands, except between the fingers.[54][56] For bat embryos, apoptosis (cell death ... Chwalkowski, F. (2016). Symbols in Arts, Religion and Culture: The Soul of Nature. Cambridge Scholars Publishing. p. 523. ISBN ...
"Plastination Technique". Körperwelten. Retrieved 2018-11-26.. *^ a b Waite FC (July 1945). "Grave Robbing in New England". ... Death and culture. *Death anniversary. *Death anxiety. *Death deity *Personification of death ... "Human body preservation - old and new techniques". Journal of Anatomy. 224 (3): 316-44. doi:10.1111/joa.12160. PMC 3931544 ... Study of the human embryo by Leonardo da Vinci. The study and teaching of anatomy through the ages would not have been possible ...
Advanced reproductive techniquesEdit. The Thoroughbred industry does not allow AI or embryo transplant. ... The types of horse bred varied with culture and with the times. The uses to which a horse was put also determined its qualities ... Embryo Transfer: This relatively new method involves flushing out the mare's fertilized embryo a few days following ... Effects of exercise on embryo recovery rates and embryo quality in the horse. Animal Repro. Sci. 94:395-397 ...
While the Dryvax virus was cultured in the skin of calves and freeze-dried, ACAM2000s virus is cultured in kidney epithelial ... The technique did have a 0.5-2.0% mortality rate, but that was considerably less than the 20-30% mortality rate of the disease ... part of a chicken embryo) and examining the resulting pock lesions under defined temperature conditions.[46] Strains were ... Society and culture. Biological warfare. The British used smallpox as a biological warfare agent at the Siege of Fort Pitt ...
McKnight JA (May 2003). "Principles of chemotherapy". Clinical Techniques in Small Animal Practice. 18 (2): 67-72. PMID ... A study in France between 1999 and 2011 came to the result that embryo freezing before administration of gonadotoxic agents to ... Production of Vindoline and Catharanthine in Multiple Shoot Cultures". In Bajaj, Y. P. S. Biotechnology in Agriculture and ... Chemotherapeutic techniques have a range of side-effects that depend on the type of medications used. The most common ...
Smith, A. F. (1994). The Tomato in America: Early History, Culture, and Cookery. Columbia SC, US: University of South Carolina ... This technique encourages the plant to send roots deep to find existing moisture in soil that retains moisture, such as clayey ... Fertilization leads to the formation of a diploid zygote that can then develop into an embryo within the emerging seed. ... In popular culture. On 30 August 2007, 40,000 Spaniards gathered in Buñol to throw 115,000 kg (254,000 lb) of tomatoes at each ...
More detailed identification techniques involve the culture of infectious agents isolated from a patient. Culture allows ... Vertical transmission, directly from the mother to an embryo, fetus or baby during pregnancy or childbirth. It can occur when ... In the absence of suitable plate culture techniques, some microbes require culture within live animals. Bacteria such as ... cannot be cultured in vitro - however the organism can be cultured in rabbit testes. It is less clear that a pure culture comes ...
A chimera is an animal (e.g., human) with double the standard DNA formed by the fusion of two embryos. Chimeras were discovered ... which is a kind of copycat technique. However, to qualify as doujinshi, "the artist must make a contribution to the art he ... The disparate features of a free culture and a permissions culture effect how culture is made. In a free culture, innovators ... A "permissions culture" is the opposite of a free culture; in a permissions culture, creators and innovators are only able to ...
Society and cultureEdit. See also: Genetics in fiction. On 19 March 2015, a group of leading biologists urged a worldwide ban ... The technique of chain-termination sequencing, developed in 1977 by a team led by Frederick Sanger, is still routinely used to ... Chinese researchers reported results of basic research to edit the DNA of non-viable human embryos using CRISPR.[109][110] ... researchers can amplify the DNA fragments by inserting plasmids into bacteria and then culturing them on plates of agar (to ...
Prospects as a therapeutic techniqueEdit. This section needs to be updated. Please update this article to reflect recent events ... The RNA interference pathway is often exploited in experimental biology to study the function of genes in cell culture and in ... Mouse oocytes and cells from early mouse embryos lack this reaction to exogenous dsRNA and are therefore a common model system ... Such techniques take advantage of the stable and heritable RNAi phenotype in plant stocks. Cotton seeds are rich in dietary ...
Dolan, Jay P. In Search of an American Catholicism: A History of Religion and Culture in Tension (2003) ... The Church also condemns embryo-destroying research and In vitro fertilization as immoral. The Church is allied with ... Catholic colleges began summer institutes to train the sisters in pedagogical techniques. Long past World War II, the Catholic ... Deck, Allan Figueroa, S.J. The Second Wave: Hispanic Ministry and the Evangelization of Cultures (New York: Paulist, 1989). ...
For example, the embryos of land vertebrates develop gill slits like fish embryos. Of course, this is only a temporary stage, ... New techniques such as 'gene targeting' are now available. The purpose of this is to insert new genes or knock out old genes ... Theoretical and empirical aspects of gene-culture coevolution. Theoretical Population Biology 59(4): 253-261. ... From the eighteenth century it was known that embryos of different species were much more similar than the adults. In ...
... including the use of chemically sterilized male lampreys in a method akin to the sterile insect technique.[86] Finally, ... which are vital during development of the embryo, but which in humans can cause problems such as cancer later in life, after ... 5 In human culture *5.1 As food. *5.2 As pests. *5.3 In folklore ... In human culture[edit]. As food[edit]. Portuguese lamprey rice ...
In cultureEdit. Main article: Insects in culture. Scarab beetles held religious and cultural symbolism in Old Egypt, Greece and ... Holometabolism, or complete metamorphosis, is where the insect changes in four stages, an egg or embryo, a larva, a pupa and ... Humans regard certain insects as pests, and attempt to control them using insecticides, and a host of other techniques. Some ... In some cultures, insects, especially deep-fried cicadas, are considered to be delicacies, whereas in other places they form ...
... was one of the pioneers in developing and modifying tissue culture techniques. The publicized work of Carrel and his associates ... Alexis Carrels Tissue Culture TechniquesAlexis Carrel, the prominent French surgeon, biologist, and 1912 Nobel Prize laureate ... Although the serial culturing technique could extend the life span of the cultures, Carrel noticed that the size of the cells ... "Alexis Carrels Tissue Culture Techniques". Embryo Project Encyclopedia (2010-06-18). ISSN: 1940-5030 ...
Embryo culture has been used to produce plants from embryos that would not normally develop within the fruit. This occurs in ... Embryo culture can also be used to circumvent seed dormancy. ... Other articles where Embryo culture is discussed: horticulture ... propagation technique. *. In horticulture: Grafting. Embryo culture has been used to produce plants from embryos that would not ...
New Techniques Detail Embryos First Hours and Days. By Jef Akst , December 1, 2017 ... New technologies reveal the dynamic changes in mouse and human embryos during the first week after fertilization. ... tags: embryos x ecology x culture x The Scientist. » embryos, ecology and culture ...
... whose embryos have vastly different culture requirements and developmental characteristics. ... This volume contains embryo culture techniques that should inspire embryologists to consider comparative studies, as the ... This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The ... Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos ...
Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo , Neural Tube Closure in Mouse Whole Embryo Culture, ... A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering, Isolation and Culture of ... culture techniques include Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow, ... Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model, Endothelialized Microfluidics for ...
New Techniques Detail Embryos First Hours and Days. By Jef Akst , December 1, 2017 ... New technologies reveal the dynamic changes in mouse and human embryos during the first week after fertilization. ...
Purchase Plant Tissue Culture: Theory and Practice, Volume 5 - 1st Edition. Print Book & E-Book. ISBN 9780444816238, ... Zygotic embryo culture. 12. Protoplast isolation and culture. 13. Somatic hybridization and cybridization. 14. Genetic ... 2. Laboratory requirements and general techniques. 3. Tissue culture media. 4. Cell culture. 5. Cellular totipotency. 6. ... The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each ...
Purchase Advances in Diagnostic and Therapeutic Techniques in Equine Reproduction, An Issue of Veterinary Clinics of North ... Breakthroughs in Embryo Cryopreservation; Collection, Maturation and Shipment of Oocytes for ART; ICSI, Embryo Culture and ... This issue of Veterinary Clinics of North America: Equine Practice focuses on Advances in Diagnostic and Therapeutic Techniques ... Advances in Diagnostic and Therapeutic Techniques in Equine Reproduction, An Issue of Veterinary Clinics of North America: ...
An in situ transgenic enzyme marker to monitor migration of cells in the mid-gestation mouse embryo. Somite contribution to the ... Culture Media * Culture Techniques * Embryo Transfer * Female * Forelimb / cytology * Forelimb / embryology* * Male ... An in situ transgenic enzyme marker to monitor migration of cells in the mid-gestation mouse embryo. Somite contribution to the ...
Embryo culture techniques‎ (21 F). *. ► Ethanol fuel‎ (3 C, 59 F). F. *. ► Function-spacer-lipid construct‎ (15 F) ...
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The efficiency with which developing sunflower embryos convert substrates into seed storage reserves was determined by labeling ... embryos with [U-(14)C6]glucose or [U-(14)C5]glutamine and measuring their conversion to CO2, oil, protein and other biomass ... which contrasts with the high rates previously determined for growing root tips and heterotrophic cell cultures. ... Carbon conversion efficiency and central metabolic fluxes in developing sunflower (Helianthus annuus L.) embryos Plant J. 2007 ...
Alexis Carrels Tissue Culture Techniques. Alexis Carrel, the prominent French surgeon, biologist, and 1912 Nobel Prize ... The Embryo Project Encyclopedia Recording and contextualizing the science of embryos, development, and reproduction.. User menu ... was one of the pioneers in developing and modifying tissue culture techniques. The publicized work of Carrel and his associates ... Fate Mapping Techniques. For more than 2000 years, embryologists, biologists, and philosophers have studied and detailed the ...
... cell positions can rapidly become a large-scale problem because cell numbers often grow exponentially in the early embryo. A ... Tissue Culture Techniques. Grant Support. ID/Acronym/Agency: 1R01HD057922/HD/NICHD NIH HHS; R01 HD057922/HD/NICHD NIH HHS; R01 ... Chick Embryo. Embryo, Nonmammalian / cytology*, metabolism*, radiation effects. Hot Temperature. Imaging, Three-Dimensional / ... using slice culture explants. The details of multiple slice culture preparation, multi-position photoactivation, and multi-time ...
... moral and political issues surrounding their derivation from human embryo ... Cells, Cultured. Cleavage Stage, Ovum / cytology, physiology. Embryo Culture Techniques. Embryo Disposition*. Embryo, Mammalian ... Cell Culture Techniques. Cell Differentiation. Cell Proliferation. Cell Separation. Cell Survival / physiology. ... Here, it is shown that arrested embryos do not resume normal development during extended culture, yet most of them contain a ...
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Female / Cells, Cultured / Culture Techniques / Embryo, ... Full text: Available Index: IMSEAR (South-East Asia) Main subject: Female / Cells, Cultured / Culture Techniques / Embryo, ... A simple technique for maintaining the lizard (Calotes versicolor) embryos in culture till hatching. ... A simple technique for maintaining the lizard (Calotes versicolor) embryos in culture till ...
... of preantral follicles and non-invasive techniques for assessing embryo vitality. ... Embryo assessment and selection for transfer. *Blastocyst cryopreservation. *Cleavage stage cryopreservation. *Culture ... Training opportunities in clinical embryology and andrology techniques are offered by Cleveland Clinics Fertility Center IVF ... efforts have resulted in the successful introduction of vitrification technology into the clinical IVF lab for human embryo ...
Mouse Preembryo Culture as an Evaluation for Human Preembryo Requirements. Oocyte and Embryo Cryopreservation Techniques and ... Sperm Washing and Concentration Techniques. The Hemi-Zona Assay (HZA): Assessment of Fertilizing Potential by Means of Human ... Organization of the In Vitro Fertilization and Embryo Transfer Laboratory. ...
Clonal Culture of Hemopoietic Cells: Techniques and Applications. Elsevier; Amsterdam, The Netherlands; 1984. ... Culture of fetal liver cells sorted for high and low levels of β galactosidase activity fromSCL lacZ/w heterozygous embryos ... Culture of fetal liver cells sorted for high and low levels of β galactosidase activity from SCL lacZ/wheterozygous embryos ... A) E7.5 embryo showing speckled staining (arrow) in the yolk sac. (B) Sagittal section of E7.5 embryo indicating that lacZ ...
Establishment in culture of pluripotential cells from mouse embryos. Nature 1981;292:154-156.. *CrossRef , ... Immunofluorescence and immunoblot were performed according to standard techniques described in supporting information Methods. ... ESC Culture. ESCs were cultured in serum+LIF complete media (CM) and 2i according to standard procedures (supporting ... Analysis of ESC culture and embryonic development provides conflicting views of the role of β-catenin-dependent, canonical Wnt ...
... and in vitro techniques (eg. protoplast culture and embryo rescue; FitzJohn et al. 2007). While in vitro approaches failed to ... Embryo culture; successful Attia et al. 1987. B. carinata x B. oleracea. Embryo culture; 6.0-7.2 embryos per pollination were ... Embryo culture; successful Attia et al. 1987. B. oleracea var. alboglabra x B. carinata. Embryo culture; 12 hybrid plants ... Successful; Embryo culture Hybrids were male sterile. Chen et al. 2012. B. carinata x B. maurorum. Embryo culture; nine hybrid ...
Specifically, our embryo culture techniques have really made a critical difference in patient outcomes. Patients are not always ... We are employing the most cutting edge techniques and culture systems. At the end of the day this makes all of the difference. ... We want our patients to know that we are employing the most cutting edge techniques and culture systems. At the end of the day ... PAFRM is having great success using a technique called vitrification to freeze both embryos and oocytes (eggs). ...
3D cell culturing technique could replace mouse embryos A gel for dosage compensation November 18, 2020 ... Early embryos as a model to study chromatin reprogramming. The degree of packaging as well as the corresponding gene activity ... With zebra-finches, infertility is mostly due to the males, while the death of embryos is due to the females ... In the Figure (left to right); i) cartoon representing the Rabl configuration of chromosomes in the early embryo. ...
3D cell culturing technique could replace mouse embryos What social distancing does to a brain December 02, 2020 ...
The UMNSAH/DF-1 cell culture presented in this section was labeled for mitochondria and the cytoskeletal filamentous actin ... a log phase adherent monolayer culture of chicken embryo fibroblast cells was fixed, permeabilized, blocked with 10 percent ... Chicken Embryo Fibroblast Cells (UMNSAH/DF-1). Visualizing the Microtubule and Filamentous Actin Networks in Chicken Embryo ... Transformed Chicken Embryo Fibroblast Cells (UMNSAH/DF-1 Line). UMNSAH/DF-1 is a spontaneously immortalized chicken (Gallus ...
Donor Sperm/Egg/Embryo Programmes *Blastocyst Culture. Involves extending the culture of the embryos for 3 more days with a ... The procedures are performed through the skin using microsurgical techniques.. Clinical services. *Surgical sperm retrieval ... It mimics more closely the natural stage of the embryo (i.e. blastocyst stage) during implantation. This programme aims to help ... Involves breaching the outer layer of an embryo by piercing a hole to facilitate its hatching out and implantation. The ...
This solution tests spent embryo culture media for chromosomal abnormalities during in vitro fertilization treatment. ... technique followed by freezing of the embryos, compared to those naturally conceived, but only in the first few weeks of life. ... embryo culture - that is part of the assisted reproduction process (ART) to placental abnormalities, risk for preeclampsia, and ... New technique reduces cell damage, improves IVF rates Motile sperm are difficult to collect with a conventional cell sorter ...
... the role played by glutathione in microspore embryogenesis consists of both antioxidative activity and stimulation of embryo- ... In: Hu H, Vega MR (eds) Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, p 431Google ... The cultures were incubated in the dark at 26 °C.. Starting from the sixth week of culture, embryo-like structures (ELS) of 1 ... Belmonte MF, Ambrose SJ, Ross ARS et al (2006) Improved development of microspore-derived embryo cultures of Brassica napus cv ...
Embryo Culture Media), Technique (Fresh Embryo IVF, Frozen Egg IVF, Donor Egg IVF) and End User (Clinics, Hospitals, Surgical ... 6.3 Frozen Embryo Transfer technique (FER). 6.3.1 Reasons to use this technique. 6.3.2 Advantages of Frozen egg IVF technique. ... TABLE 36 EMBRYO CULTURE MEDIA BY VARIOUS COMPANIES. TABLE 37 GLOBAL IVF EMBRYO CULTURE MEDIA MARKET BY GEOGRAPHY, 2014-2020 ($ ... 12 COMPARISON OF THE MEDIA USED IN EMBRYO CULTURE. FIG. 13 SUCCESS RATE OF IVF TECHNIQUES PERFORMED BY AGE, 2013, U.S.. FIG. 14 ...
Blastocyst culture. Normal cost: £425-495 , Cost with package: Free A blastocyst is an embryo that has been developed for ... Additional techniques you receive as part of the package:. CAREmaps time-lapse technology. Normal cost: £775 , Cost with ... Transfer of all embryos (including unlimited frozen embryos transfers in subsequent cycles) ... As the embryo is at a more advanced stage, we can identify the best embryo to transfer, increasing the chance of successful ...
  • Oocyte and Embryo Cryopreservation Techniques and Results. (
  • Our latest research efforts have resulted in the successful introduction of vitrification technology into the clinical IVF lab for human embryo cryopreservation. (
  • Based on reagents & media, the market is categorized into, cryopreservation media, semen processing media, ovum processing media and embryo culture media. (
  • Cryopreservation or freezing of embryos is another technique that can be used to reduce multiple births. (
  • Cryopreservation is widely available and can help decrease the pressure to transfer large numbers of embryos by allowing a couple to freeze extra embryos for use at a later date. (
  • The disadvantages of cryopreservation are that some embryos will not survive the freeze-thaw process and previously frozen embryos may have less pregnancy potential than "fresh" embryos. (
  • With increased success rates of ovarian stimulation, oocyte retrieval and invitro fertilization and development, the problem of dealing with excess oocytes and embryos has been tackled effectively by cryopreservation. (
  • The book's main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. (
  • It mimics more closely the natural stage of the embryo (i.e. blastocyst stage) during implantation. (
  • When oocyte cumulus cell-oocyte-complexes (COCs) were cultured in maturation medium containing 10% of each individual FF, the rate of development to the blastocyst stage was significantly correlated with the number of GCs in the donor gilt. (
  • The embryos were developed only to the blastocyst stage, at which point they were studied in processes that destroyed them. (
  • We investigated 263 cycles of vitrified-warmed blastocyst stage embryo transfer performed between April 2005 and April 2009, which were reviewed retrospectively. (
  • Cycles of vitrified-warmed blastocyst stage embryo transfer performed between April 2005 and April 2009 were reviewed retrospectively. (
  • The blastocyst stage requires advanced incubation techniques with low oxygen incubators and specialized culture media. (
  • The blastocyst stage is a more advanced stage in which the genetic code of the embryo is fully activated and working. (
  • A young woman (under age 35) with high quality blastocyst stage embryos and a healthy uterus can reliably transfer a single embryo and achieve high pregnancy rates. (
  • Pellicer, A. The effect of pronuclear morphology on early development and chromosomal abnormalities in cleavage-stage embryos. (
  • ES cell lines are conventionally isolated from the inner cell mass of blastocysts and, in a few instances, from cleavage stage embryos . (
  • A more recent technique, called vitrification, was found to be most effective for freezing cleavage stage embryos from in-vitro produced tiger embryos with an approximately 50% survival rate post-thawing. (
  • P19 cells, which exhibit epithelial morphology and adherent growth in culture, have been of significant interest to developmental biologists. (
  • Through this process the vigor and morphology of the embryos is improved and the tendency to germinate prematurely is significantly reduced. (
  • One of the distinctive features of orchid embryos is the diverse morphology of their suspensors. (
  • In 1949, Swamy devised a classification scheme for orchid embryo development based on suspensor morphology alone. (
  • Nuhoglu, A. The effect of pronuclear morphology on embryo quality parameters and blastocyst transfer outcome. (
  • Tews, G. Elective transfer of embryos selected on the basis of first polar body morphology is associated with increased rates of implantation and pregnancy. (
  • Currently, the selection of cow embryos for ET takes place on the basis of morphology and development stage. (
  • and the evaluation of embryos of women with repeated, unexplained IVF failure despite good embryo morphology. (
  • New technologies reveal the dynamic changes in mouse and human embryos during the first week after fertilization. (
  • Organization of the In Vitro Fertilization and Embryo Transfer Laboratory. (
  • A new study published in the journal Fertility and Sterility in February 2020 reports a higher risk for mortality in children who were conceived by the in vitro fertilization (IVF) technique followed by freezing of the embryos, compared to those naturally conceived, but only in the first few weeks of life. (
  • This solution tests spent embryo culture media for chromosomal abnormalities during in vitro fertilization treatment. (
  • It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). (
  • 21st National level training programme on 'In-Vitro Fertilization and Embryo Co-Culture: TANUVAS, Chennai, India. (
  • In vitro fertilization (IVF) is the process of fertilising an egg with a sperm in the laboratory to produce an embryo. (
  • in vitro fertilization vē´trō, vĭ´trō [ key ] (IVF), technique for conception of a human embryo outside the mother's body. (
  • In vitro Fertilization with Rice Gametes: Production of Zygotes and Zygote and Embryo Culture Takashi Okamoto 3. (
  • Gomel, V. The effect of embryo quality on subsequent pregnancy rates after in vitro fertilization. (
  • He worked systematically to realize his goal, discovered important principles for human fertilization, and succeeded in accomplishing fertilization of human egg cells in test tubes (or more precisely, cell culture dishes). (
  • The lectures focus on germline stem cells and their differentiation into functional gametes through the processes of spermatogenesis and folliculogenesis, maturation of gametes and acquisition of fertilization and developmental competence, sperm-egg interaction, egg activation and preimplantation embryo development. (
  • The labs in Section 2 focus on stem cell transplantation and meiotic staging of germ cells, follicular culture and in vitro techniques related to maturation of oocytes, sperm physiology, fertilization of various animal models, intracytoplasmic sperm injection, and other micromanipulation procedures. (
  • The Reproductive Sciences Department, in collaboration with Omaha's Henry Doorly Zoo and Aquarium's veterinary and animal crews, has developed techniques such as artificial insemination and in vitro fertilization for many species and maintains a bank of more than 20,000 samples of frozen reproductive cells from over 50 species. (
  • The Tiger Project has been a long-term program of the Reproductive Sciences Department that has focuses on all aspects of assisted reproductive technology including artificial insemination, semen freezing, in vitro embryo production (including fertilization by intracytoplasmic sperm injection), freezing and transfer, and the production of tiger-specific hormones for stimulating and synchronizing ovarian activity in tigers. (
  • Interspecific incompatibility in plants can occur for many reasons, but most often embryo abortion occurs In plant breeding, wide hybridization crosses can result in small shrunken seeds which indicate that fertilization has occurred, however the seed fails to develop. (
  • The experiments, reported in Nature magazine, used in vitro fertilization techniques to take DNA from a mother and father, but insert it in an egg furnished by another woman. (
  • The media and techniques we use in sperm preparation is designed to select a significant number of motile, morphologically normal and viable sperm for the eventual fertilization process. (
  • 1989). Several peer-reviewed journals now devote major attention to the clinical and applied aspects of IVF -Fertility and Sterility, Human Reproduction , and Journal of In Vitro Fertilization and Embryo Transfer . (
  • Human In Vitro Fertilization and Embryo Transfer ( D. P Wolf and M. M. Quigley, eds. (
  • Then the embryo develops directly from the egg without fertilization. (
  • however subsequent abortion of the embryo that began growing following fertilization leads to a near seedless condition. (
  • Says Founder and Medical Director, Dr. Andrew Levi , "Unlike other large volume-driven IVF centers, we are able to recommend IVF protocols to patients that are more personalized, are focused on both embryo quality and embryo implantation, and that ultimately will lead to higher pregnancy and live-birth rates. (
  • Involves breaching the outer layer of an embryo by piercing a hole to facilitate its hatching out and implantation. (
  • As the embryo is at a more advanced stage, we can identify the best embryo to transfer, increasing the chance of successful implantation. (
  • Implantation rate is the potential of a single embryo to result in a pregnancy and is calculated by taking the total number of gestational sacs divided by the total number of embryos replaced. (
  • Implantation rate is always lower than the pregnancy rate because the vast majority of IVF procedures involve the transfer of more than one embryo. (
  • The higher the implantation rate, the lower the number of embryos needed to achieve pregnancy, and the lower the multiple birth rate. (
  • Again, improvements in implantation rates will ultimately allow physicians to replace fewer and fewer embryos, maintain or improve the overall pregnancy rate and someday, eliminate the risk of high order multiple pregnancy. (
  • Because of the higher rate of chromosomal abnormalites, the implantation rate of the embryos decreases. (
  • By using PGD to select the chromosomally normal embryos, the implantation rate of the embryos that are selected is increased. (
  • Embryo screening procedures have also been used to identify embryos that are generally free of abnormalities and are not likely to result in a miscarriage after implantation, thus improving the likely success of IVF. (
  • In embryo donation (also called embryo adoption), frozen embryos that are not needed by the mother are donated for implantation to a woman or couple who are infertile but wish to have, and are capable of bearing, children. (
  • Here we report an alternative method of establishing ES cell lines - using a technique of single-cell embryo biopsy similar to that used in pre-implantation genetic diagnosis of genetic defects - that does not interfere with the developmental potential of embryos. (
  • There were no differences among the vitrified day-5, day-6 and day-7 blastocyst transfer groups regarding mean number of embryos transferred, pregnancy rate, implantation rate and miscarriage rate. (
  • These results suggest that differences in pregnancy rate between fresh and cryopreserved day-6 blastocysts attributed to reduced embryo quality with slower development may instead result from impaired endometrial receptivity by the time more slowly growing embryos develop to the stage at which implantation is possible. (
  • The hybrid embryos have developed enough for implantation, and have now been frozen while scientists seek potential surrogate southern white rhino females to carry them to term. (
  • Expression of aurora C decreases during late blastocyst stages resulting in post-implantation defects in aurora B-null embryos. (
  • Our philosophy further relies on state-of-the-art IVF technology to improve your chances of conception and successful embryo implantation. (
  • An optimal culture also plays a critical role in the IVF journey from retrieval of the egg through implantation of the embryo. (
  • In vitro embryo production efficiency in cattle and its association with oocyte adenosine triphosphate content, quantity of mitochondrial DNA, and mitochondrial DNA haplogroup. (
  • Elder, K. Laboratory techniques: oocyte collection and embryo culture. (
  • Last year, in 2007, our program of single embryo transfer in oocyte donation recipients produced a 66% pregnancy rate. (
  • Today half of our patients using oocyte donation elect to transfer a single embryo. (
  • The technique of oocyte donation was initially developed for patients with agonadism (e.g. premature ovarian failure. (
  • Currently, oocyte donation is most often used for the treatment of patients of advanced reproductive age or those who have repeatedly produced embryos of poor quality. (
  • Current projects include vitrification, assessment of growth factor influence on blastocyst differentiation, embryonic cell death and apoptosis, in vitro maturation (IVM) of preantral follicles and non-invasive techniques for assessing embryo vitality. (
  • Culture techniques: Applicability for studies on prenatal differentiation and toxicity: 5th symposium on prenatal development, May, 1981, Berlin. (
  • Shh increased the percentage of ciliated motor neurons, especially in G93A SOD1 culture.Moreover, Shh enhanced cell survival and differentiation of motor neuron precursors in WT culture.Shh is neurotrophic to motor neurons and has mitogenic effects in WT and mSOD1 G93A culture in vitro. (
  • Moreover, Shh enhanced cell survival and differentiation of motor neuron precursors in WT culture. (
  • Here, we use a stringent experimental strategy to molecularly define the astrocyte lineage by integrating microarray datasets across several in vitro model systems of astrocyte differentiation, primary astrocyte cultures, and various astrocyterich CNS structures. (
  • During the early globular stage of embryo development, structural differentiation occurs, revealing an abundance of smooth endoplasmic reticulum cisternae and wall ingrowths within the suspensor cells. (
  • Cell growth and differentiation in each culture were examined by phase contrast microscopy and described both qualitatively and quantitatively. (
  • The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells in vitro and in vivo . (
  • Non-viable human embryos as a source of viable cells for embryonic stem cell derivation. (
  • However, the extraordinary potential of hESC as therapeutic tools is tempered by ethical, moral and political issues surrounding their derivation from human embryos. (
  • It has previously been proposed that ethical criteria applied to essential organ donation could be employed for derivation of hESC from irreversibly arrested, and thus organismically dead, human embryos produced during routine IVF procedures. (
  • 5 viable cells), suggesting that this class of non-viable embryos could be a rich source of viable cells for derivation of hESC lines. (
  • Goldfarb, J. M. Morphological evaluation of human embryos and derivation of an embryo quality scoring system specific for day 3 embryos: a preliminary study. (
  • The most basic objection to human embryonic stem (ES) cell research is rooted in the fact that ES cell derivation deprives embryos of any further potential to develop into a complete human being. (
  • 3 Human embryo culture for human embryonic stem cell derivation. (
  • Timing mainly refers to the maturation stage of the embryo before excision. (
  • In addition to the utilization of novel stimulation protocols, PAFRM continues to focus on elective single embryo transfer (eSET). (
  • Single Embryo Transfer (SET) is an effective way of minimizing the risk of multiple gestations, but an accurate method of selecting the most viable embryo will optimise the use of SET. (
  • Ultimately, as the technique improves, PGD may allow us to offer all patients a single embryo transfer, eliminating the multiple pregnancy problem without sacrificing a high pregnancy rate. (
  • The single embryo culture technique and a histochemical staining method were employed to distinguish male embryo cultures from female ones. (
  • Utilizing a single embryo, two-thirds of patients were able to conceive a singleton pregnancy. (
  • Single embryo transfer is not always possible. (
  • The two biologists also expanded the application of tissue culture to tissues of various types, including adult tissues, tissues of mammalian origin, and cancerous tissues from chicks and humans . (
  • The technique involves testing isolated mammalian embryos in culture by direct exposure to agents. (
  • In general, the results of the study indicate that young mammalian embryos are responsive to a variety of contaminants that may be found in their environment. (
  • It is concluded that the procedure as reported is capable of determining the type of effect that a contaminant will have on the development of the mammalian embryo. (
  • Especially, collagen-gel embedded culture of bacteria and mammalian cells with ultra-small volume (tens to several nL) has been successfully demonstrated on glass or silicon chip to microfabricate various types of cellular arrays. (
  • Sperm Washing and Concentration Techniques. (
  • We collect eggs from your donor and fertilise them, using the sperm of your partner, or a sperm donor, and we'll then monitor the embryos and choose the best time to place back in the womb. (
  • It is also used to enable prospective parents with other reproductive problems (e.g., inability to produce eggs, poor sperm quality, or endometriosis ) to bear a child, and can be used in conjunction with embryo screening to enable parents to have a child who is free of some inheritable defects or diseases. (
  • Spermatozoa were acquired by microepididymal sperm aspiration and processed using swim-up technique. (
  • Steptoe used the laparoscope to remove eggs from the ovaries and Edwards put the eggs in cell culture and added sperm. (
  • The program also produced the first artificially inseminated snake species, and continues leading assisted reproduction efforts in captive gorillas by utilizing sperm sex-sorting techniques in order to skew the sex ratio to produce more female offspring. (
  • Sperm and in vitro produced tiger embryo procedures have been successful, however, pregnancy rates have been less than satisfactory using the porcine hormones to synchronize ovarian cycles. (
  • Pregnancy requires the cooperation of sperm and egg, accurate transcription of the early genetic code in the developing embryo, a fertile spot for attachment to the mother in the uterus, and a route for getting there. (
  • LONDON (Reuters) - Scientists have created hybrid embryos from the sperm of near-extinct northern white rhinoceroses in the laboratory, hoping they can ultimately help save the species. (
  • The low-grade sperm have to be activated with a lab culture so that they can be used in an IVF technique known as intracytoplasmic injection. (
  • Lastly, the premium quality medium can be used for other sperm preparation techniques such as diluting gradient solutions, washing, and performing sperm counts. (
  • Plasma generally predicted pregnancy and birth better than CM. These first results show that FTIR metabolomics could allow the identification of embryos and recipients with improved pregnancy viability, which may contribute to increasing the efficiency of selection schemes based on ET. (
  • Therefore, improving embryo viability prediction would increase the efficiency of ET programs. (
  • However, gene expression techniques are not extensively used on field, and biopsy, when associated with freezing in particular, may compromise embryonic viability [ 1 , 8 , 9 ]. (
  • The analysis of the culture medium (CM) that surrounds the embryo represents a noninvasive alternative in the search for markers associated with embryo viability. (
  • Breeding of incompatible interspecific and intergeneric species Overcoming seed dormancy Determination of seed viability Recovery of maternal haploids that develop as a result of chromosome elimination following interspecific hybridization Used in studies on the physiology of seed germination and development Depending on the organ cultured, it may be referred to as either embryo, ovule, or ovary culture. (
  • Embryo viability and increased chances for a successful outcome are often influenced by nutrients and other factors. (
  • To improve embryo viability we use premium quality media at each stage of IVF journey. (
  • In 1910, Carrel's assistant, Montrose Burrows, visited Harrison's laboratory at Yale University for several months to learn about tissue culture. (
  • 2. Laboratory requirements and general techniques. (
  • Says Dr. Bryan Kloos, PhD, HCLD, Lead Embryologist at PAFRM, "We have made changes over the short- and long-term in our IVF laboratory that have significantly led to improved embryo quality, thus leading to a greater chance of a patient achieving a successful pregnancy. (
  • More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. (
  • Improvements in various techniques used in the laboratory have led to gradually improving pregnancy rates across the country. (
  • 1 Organization and good aseptic technique in the human embryonic stem cell laboratory. (
  • Both plant and animal cells, tissues and organ culture is possible in artificial nutrient medium in controlled laboratory conditions. (
  • These high-quality serological pipettes have been tested to meet IVF laboratory requirements for gametes and embryos. (
  • Raghavan, V.: Nutrition, growth and morphogenesis of plant embryos. (
  • Embryo rescue is one of the earliest and successful forms of in-vitro culture techniques that is used to assist in the development of plant embryos that might not survive to become viable plants. (
  • Plant embryos are multicellular structures that have the potential to develop into a new plant. (
  • The most widely used embryo rescue procedure is referred to as embryo culture, and involves excising plant embryos and placing them onto media culture. (
  • A detailed study of embryo development would provide information on subsequent germination events and aid in the propagation of the species. (
  • A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. (
  • Carrel's long investigation in improving tissue culture methods was thus initiated. (
  • Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation. (
  • Methods and cell lines for cloning ungulate embryos and offspring, in particular bovines and porcines, are provided. (
  • The overall purpose of the present study is to investigate methods for selection of the best embryo. (
  • In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. (
  • Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes. (
  • Traditional methods for cryopreserving (freezing) embryos from livestock or humans can work with domestic cat embryos, but do not necessarily work for exotic felids such as tigers. (
  • Improvements in insemination technique, embryo culture methods, and transfer efficiency have added substantially to pregnancy rates. (
  • We have developed proprietary methods for growing, maintaining, and scale-up of undifferentiated hESCs using feeder cell-free conditions and a chemically defined culture medium," continues Dr. Okarma. (
  • Not All Egg & Embryo Freezing Methods Are Alike. (
  • 11. Zygotic embryo culture. (
  • After a period of several weeks in culture somatic embryos having the appearance of zygotic embryos will have formed. (
  • Part I: Culture of Zygotic Embryos: Fundamental Studies 1. (
  • Zygotic Embryo Culture: An Overview Tegan M. Haslam and Edward C. Yeung 2. (
  • Canola Zygotic Embryo Culture Nicole S. Ramesar-Fortner and Edward C. Yeung 4. (
  • In vitro Culture of Coconut (Cocos nucifera L.) Zygotic Embryos Florent Engelmann, Bernard Malaurie, and Oulo N'Nan Part II: Application of Embryo Culture Techniques: Embryo Rescue 7. (
  • Such improvements have enabled us to think about ways to reduce the risk of multiple pregnancy by transferring fewer embryos. (
  • Pacific Fertility Center has pioneered techniques of transferring fewer embryos. (
  • The nuclei are reprogrammed to direct the development of cloned embryos, which can then be transferred into recipient females to produce fetuses and offspring, or used to produce cultured inner cell mass cells (CICM). (
  • The aim of the present study was to compare the efficiency of in vitro production and in vitro development of cloned sheep embryos by the two techniques. (
  • IVF devices and consumables market by techniques is categorized into, fresh embryo IVF, frozen egg IVF and donor egg IVF. (
  • However, there does not appear to be any increase in the rate of birth defects or miscarriages in pregnancies conceived using frozen-thawed embryos. (
  • Nevertheless, the technique has raised legal, ethical, and religious issues, including concerns regarding legal custody of frozen embryos following divorce and questions regarding the appropriateness of the procedure posed by the Roman Catholic Church and other institutions. (
  • Trounson reported the first human pregnancy following thawing of frozen embryos in 1983. (
  • But what is still not talked about is the fact that these cells (blastomeres) isolated by biopsy can also revert to new embryos in vitro - and then be used as a continuous source of new human "biological" materials for research, or be implanted, allowed to develop to term, and be born. (
  • Dr. Levi adds, "One of the ways that we are able to attain such high pregnancy rates is by using a technique called Comprehensive Chromosome Screening , known as CCS. (
  • Using CCS, we are able to test and subsequently identify chromosomally normal embryos for transfer, thus maximizing pregnancy rates without placing a patient at significant risk for twins. (
  • All other factors being equal, pregnancy rates almost double when two embryos are transferred instead of one, and increase again when a third and fourth embryo are added. (
  • The desire for high pregnancy rates has driven a desire for more embryos to be transferred 7 . (
  • This pregnancy rate was very similar to the overall pregnancy rates regardless of the number of embryos transferred. (
  • The current increase in the use of embryos selected for their genetic merit and the need for high fertility recipients [ 1 , 2 ] has made the improvement of pregnancy rates upon embryo transfer (ET) a major objective in cattle farming. (
  • Alexis Carrel , the prominent French surgeon, biologist, and 1912 Nobel Prize laureate for Physiology or Medicine, was one of the pioneers in developing and modifying tissue culture techniques. (
  • The publicized work of Carrel and his associates at the Rockefeller Institute established the practice of long-term tissue culture for a wide variety of cells. (
  • At the same time, some aspects of their work complicated the operational procedures of tissue culture. (
  • Thus Carrel's legacy had a mixed influence on the development of tissue culture techniques, which have been widely used in the fields of embryology and stem cell research. (
  • Carrel's work with tissue culture followed the research of Ross Granville Harrison . (
  • In 1907 Harrison successfully carried out the first tissue culture using the hanging drop method , in which fragments of embryonic frog nerve tissue were grown in a clot of lymphatic fluid. (
  • Between October 1910 and March 1911, Carrel and Burrows published four papers on tissue culture in the Journal of the American Medical Association . (
  • Conceptually, they coined the term "tissue culture" and gave its definition as "a plasmatic medium inoculated with small fragments of living tissue. (
  • Although the method they used was generally based on Harrison's original design, Carrel and Burrows substituted blood plasma for lymph in the tissue culture medium . (
  • This material change made the tissue culture less laborious and more reliable, because chick blood plasma was easier to obtain and more homogeneous in quality, compared to lymph fluid. (
  • Among many speculations proposed to account for how Carrel's chick heart tissue could have survived in tissue culture for so long, was the question of whether the "embryo juice" was in fact introducing extra living cells that had not been eliminated by the centrifugation and filtration processes. (
  • 3. Tissue culture media. (
  • Glossary of terms commonly used in plant tissue culture. (
  • Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. (
  • The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. (
  • This book includes three new chapters: 'Variant selection', 'Genetic Engineering' and 'Production of Industrial Compounds' and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature. (
  • Presents updated information on the theoretical, practical and applied aspects of plant tissue culture based on more than 2500 references. (
  • the book can serve as everybody interested in plant tissue culture. (
  • 5. A tissue culture of regenerable cells of the plant of claim 2 . (
  • 7. A cotton plant regenerated from the tissue culture of claim 5 , wherein the regenerated cotton plant expresses all of the physiological and morphological characteristics of cotton variety 04T048, a representative sample of seed of said variety having been deposited under ATCC Accession No. PTA-9900. (
  • The invention is a method for developing tissue culture induced coniferous somatic proembryos into well developed cotyledonary embryos. (
  • Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. (
  • Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. (
  • Cloning, embryo transfer and tissue culture are also key areas we cover. (
  • P. R. White desribed a simple technique in his book on tissue culture in which embryos removed from the seeds of Shepherd's Purse (Capsella bursa-pastoris) can be cultured under completely aseptic conditions. (
  • In: Plant Cell and Tissue Culture. (
  • Many desired products can be obtained through tissue culture like pharmaceutical drugs, vaccines, and monoclonal antibodies. (
  • Plant embryogenesis Callus (cell biology) Plant tissue culture Plant hormone Hyperhydricity Somatic embryogenesis Sage, T.L. (
  • Before the tiny embryo aborts, it is removed from the developing fruit and grown in tissue culture until it is large enough to survive on its own. (
  • This involves developing an embryo in the lab for longer (five or six days) than in a standard IVF cycle (two or three days). (
  • These features are not present in cells of the embryo proper. (
  • The maize chromosomes are found in varying numbers in the first cells of the embryo but are then selectively eliminated to leave only the wheat genomic complement. (
  • We offer this package in conjunction with Access Fertility, who will refund you up to 50% of your fees if your treatment fails after your IVF donor egg cycles and associated embryo transfers have been completed. (
  • In addition, when GCs were cultured in medium containing FF, the proliferative activity of the GCs was also significantly correlated to the number of GCs in the donor gilt. (
  • First, the donor cells are grown under special conditions in culture. (
  • Factors affecting germination of immature embryos were evaluated. (
  • The immature embryos collected 11-16 d after pollination expressed the maximum germination rate (100%), but the maximum efficiency of recovery was observed at 15-16 d after pollination. (
  • The germination time of embryos increased and length of the germinated shoots decreased as the age of the embryos increased. (
  • G. S. LIU , D. M. QI , W. D. ZHANG , J. S. LIU , and H. J. LI "HIGHLY EFFICIENT EMBRYO GERMINATION IN VITRO SHORTENS THE BREEDING CYCLE IN LEYMUS CHINENSIS ," In Vitro Cellular and Developmental Biology - Plant 40(3), 321-324, (1 May 2004). (
  • In vitro Culture and Germination of Terrestrial Asian Orchid Seeds Yung-I Lee 6. (
  • The success rates in KKIVF are expressed as the percentage of embryo transfer procedures which result in a clinical pregnancy. (
  • A clinical pregnancy is determined when a pregnancy sac with a heartbeat is visible on ultrasound at around 4 weeks after the embryo transfer procedure. (
  • In 2004 and 2005, Hwang Woo-suk , a professor at Seoul National University , published two separate articles in the journal Science claiming to have successfully harvested pluripotent, embryonic stem cells from a cloned human blastocyst using somatic-cell nuclear transfer techniques. (
  • Conclusions The morphological features of the transfer cell and the absence of cuticular material in the suspensor cell wall corroborate the hypothesis that the suspensor is the major nutrient uptake site for the developing embryo in the lady's slipper orchid. (
  • In general, the best way to reduce multiple births is to transfer only single embryos. (
  • Preimplantation genetic diagnosis (PGD), or the genetic analysis of embryos prior to transfer into the body is an exciting new technology that could help reduce multiple births. (
  • The risk of the transmission of BTV by bovine embryos is negligible if the embryos are washed according to the International Embryo Transfer Society (IETS) protocol. (
  • Devroey reported the first pregnancy and birth by the technique of Zygote Intrafallopian Transfer in 1986. (
  • Transfer of gamate/embryo and surrogate motherhood. (
  • Shah, RA 2018-01-01 00:00:00 Conventional somatic cell nuclear transfer (SCNT) technique of in vitro production of cloned embryos involves use of costly and complicated micromanipulators. (
  • On the other hand, little published information is available regarding the transfer of day-7 blastocysts because embryos developing slowly are generally considered nonviable and are therefore discarded. (
  • For use in ovarian stimulation and synchronization for cat embryo transfer. (
  • The development of blastocyst (day 5 embryo) culture techniques allows the selection of high quality embryos for transfer. (
  • Only the healthiest of embryos can move to the more advanced stages, allowing selection of the best embryos for transfer. (
  • These guidelines encourage all of us to transfer just enough embryos to achieve pregnancy. (
  • An older woman (over 40) may need to transfer 3 or more embryos to achieve a good pregnancy rate. (
  • Embryos collected on Day 6 from superovulated cows in 2 countries were individually cultured in synthetic oviduct fluid medium with BSA for 24 h before embryo transfer. (
  • Lab techniques similar to making ESCs via NT, Nuclear Transfer. (
  • And just in case somebody else is trying to raise a religious argument against nuclear transfer - suggesting that human embryos are at risk - please check out a magnificent book "THE LANGUAGE OF GOD, a Scientist presents Evidence for Belief," by one of our most renowned scientists, Dr. Francis Collins of NIH. (
  • Patients with congenital absence of the uterus (Mayer-Rokitansky-Hauser syndrome) or patients who have undergone hysterectomy can undergo IVF with embryo transfer to a gestational surrogate resulting in birth of genetic offspring. (
  • PGD allows the detection of significant genetic disease before embryo transfer and conception. (
  • A sounding (trial transfer) of the endometrial cavity for depth, position, and accessibility should also be performed, once negative cultures have been obtained. (
  • About 15% of egg retrieval cycles end in pregnancy and live birth resulting from the transfer of, on average, three embryos. (
  • PGD can be used to look for chromosomal aneuploidy (abnormal numbers of chromosomes) in the embryo. (
  • The quality and number of oocytes diminish with age, and the rates of aneuploidy increase in resultant embryos. (
  • Handling of gametes and embryos can sometimes be a significant challenge. (
  • This ensures that embryos and gametes will be immersed in optimal conditions. (
  • The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics. (
  • It is virtually impossible to talk about the conservation and sustainable use of genes, species and ecosystems separate from human cultures. (
  • Over the years the excised embryos of many species have been brought into culture using relatively simple nutrient media. (
  • Handmade cloning (HMC) technique has been applied as efficient and cost‐effective alternative in many livestock species. (
  • Current efforts also include projects to develop assisted reproductive techniques in amphibian species. (
  • The temperature and light requirement is generally species specific and thus its usually regulated to be the within the same temperature requirement as that of its parent with embryos of cool-season crops requiring lower temperatures than those of warm-season crops. (
  • Mouse Preembryo Culture as an Evaluation for Human Preembryo Requirements. (
  • In January 2008, Dr. Andrew French and Samuel Wood of the biotechnology company Stemagen announced that they successfully created the first five mature human embryos using SCNT. (
  • In this case, each embryo was created by taking a nucleus from a skin cell (donated by Wood and a colleague) and inserting it into a human egg from which the nucleus had been removed. (
  • In the first approach, gas chromatography-negative chemical ionization/mass spectrometry (GC-NCI/MS) of the thiohydantoin sample subsequent to the modified Edman degradation was performed using a thermodesorption/cold trap (TCT) injection technique (detection limit for in vitro exposure of human blood to sulfur mustard: 30 nM). (
  • To study the effects of a specific group of hormones (all ligands of the steroid/retinoid/thyroid hormone receptor superfamily) on the angiogenic process in humans, we have used a model system in which human microvascular endothelial cells from foreskin (hMVEC) are cultured on top of a human fibrin matrix in the presence of basic fibroblast growth factor and tumor necrosis factor-α. (
  • When it comes to intellectual property claims on the human genome and assorted human biotechnology techniques, the horse left the lab a long time ago. (
  • The ability to generate human ES cells without the destruction of ex utero embryos would reduce or eliminate the ethical concerns of many. (
  • What is finally talked about in this article is the well-known scientific fact that many early human embryos diagnosed as "damaged" (e.g., after freezing and thawing), or that undergo prenatal genetic diagnosis, could heal themselves, be implanted, and be born normal. (
  • 2 What is also talked about is that the separated cells (blastomeres) biopsied or separated from the whole early human embryo can go on to produce human embryonic "stem cell" lines. (
  • It is precisely the natural biological process of "regulation" and the "totipotent" properties of all early human embryonic "stem cells" which explain to a great extent what makes possible such early embryonic "healing" as reported in this recent study, as well as explain the reversion of early embryonic cells to new embryos . (
  • It has been well known for decades that " regulation " is in large part responsible for both the healing of damaged early human embryos as well as for the reversion of their separated totipotent cells (blastomeres) to new human embryos . (
  • To investigate the development potential of blastomeres isolated from 4-cell human embryos . (
  • RESULTS: 83 blastomeres were isolated from 20 4-cell and 2 3-cell human embryos. (
  • Previous investigators have suggested that the age of blastocyst is indicative of embryo quality among human embryos derived from assisted reproductive technology. (
  • The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches. (
  • Section 1: Obtaining and culturing human embryonic stem cells. (
  • 5 Standard culture of human embryonic stem cells. (
  • 6 Culture of human embryonic stem cells in chemically defined conditions. (
  • Researchers in the US and England are reporting initial success in experiments in which they produced human embryos from three adult donors. (
  • 1953. Distribution and excretion of radioactive thallium in the chick embryo, rat, and man. (
  • Two generations were obtained within 2 yr starting from immature embryo cultures, as compared to one generation using conventional breeding techniques. (
  • Immature Embryo Rescue and Culture Xiuli Shen, Fred G. Gmitter, Jr., and Jude W. Grosser 8. (
  • Intraembryonic vasculogenesis in precirculation SCL lacZ/lacZ embryos appeared normal but, in embryos older than embryonic day (E) 8.5 to E9, absolute anemia leading to severe hypoxia precluded an accurate assessment of further vascular development. (
  • Primary NSCs were isolated from murine embryonic day (E)13.5 embryos as described ( 13 ). (
  • In addition to age, results will depend on the condition of the uterus, the number of eggs obtained, the number of eggs fertilised, and the number of eggs or embryos transferred. (
  • The embryo grows for a few days in an incubator before being transferred into the uterus. (
  • The embryos were destroyed, however, before being implanted in a woman's uterus. (
  • The theory has been posited that perhaps the damaged tube is unable to expel or push the embryo back down into the uterus after it has found its way into the tube. (
  • It may be due to the technique used in the transference of the embryo to the uterus. (
  • If the embryo is placed too high into the uterine cavity, it may be forced up into the fallopian tube and, since the tube may be damaged, it is caught and unable to return to the uterus. (
  • Department lab rotation to understand advanced reproductive techniques in the filed of Reproductive Biology. (
  • Simultaneously, advances in assisted reproductive techniques have led to new possibilities for the prevention and treatment of infertility. (
  • The authors have not forgotten to pay particular attention to new developments in somatic embryogenesis, industrial and agricultural applications of in vitro culture, variant selection, production of secondary metabolites, genetic engineering application of bioreactors, and fermetor techniques. (
  • a number of techniques can be used to replace the mother's mitochrondria, but all involve transferring nuclear genetic material from the mother's egg to a donor's egg from which that material has been removed. (
  • He hopes the technique can help us understand how eggs develop, and learn more about the effects of genetic mutations on fertility. (
  • Whereas genetic ablation of survivin, borealin or incenp results in early lethality at the morula stage, we show here that aurora B is dispensable for CPC function during early cell divisions and aurora B-null embryos are normally implanted. (
  • This occurs because a higher proportion of the embryos are chromosomally abnormal and because most chromosomal abnormalities do not cause any changes in the embryos appearance. (
  • All multicellular animals utilize Notch signaling, which contributes to the formation, growth, and development of embryos (embryogenesis). (
  • Depending on the capability for stress adaptation, the role played by glutathione in microspore embryogenesis consists of both antioxidative activity and stimulation of embryo-like structure development. (
  • The process of microspore embryogenesis (ME) is an alternative pathway of development, available for in vitro cultured immature pollen grains (microspores), which leads to the formation of haploid embryo-like structures (ELSs), able to regenerate haploids/doubled haploids (DHs). (
  • The fruits contain a seed single, which is large, globose to pyriform with two seed coats and two large firm, almost hemi-spherical cotyledons, 2-4 cm in diameter enclosing a small embryo. (
  • It becomes necessary to excise the entire small embryo to prevent early embryo abortion. (
  • Comparison of efficiency of in vitro cloned sheep embryo production by conventional somatic cell. (
  • 05). It was inferred that HMC technique provides a cost‐effective and efficient method of in vitro production of cloned sheep embryos with a comparatively simpler technique with a possibility of automation. (
  • GREEN POD CULTURE: Time for Pod to Remain on Plant 100 days after pollination is sufficient for many genera. (
  • Chickpea Hybridization Using In vitro Techniques Nalini Mallikarjuna and Fred J. Muehlbauer 9. (
  • Still other concentrations will interfere with the metabolic function of the embryo. (
  • We hypothesise that the quality of the embryo is reflected by the transcription of selected genes, the cleavage kinetics, and the metabolic profile. (
  • Studies on the strong metabolic relationships which exist between embryo and endosperm, or embryo and cotyledon(s), are also possible using excised embryos in culture. (
  • Preparation and maintenance of oviduct epithelial cells, cumulus cells etc., for embryo co-culture. (
  • Along with this increase in knowledge has emerged new and advanced laboratories performing unique techniques aimed at diagnosing and treating infertility problems. (
  • Currently, there are no markers fully predictive of developmental competence of IVF embryos. (
  • Nearly everyone actually involved in stem cell research, including those troubled by embryo destruction, says that ESC research is still necessary . (
  • Using a well-established technique , the researchers turned these cells into induced pluripotent stem cells - cells that can continue to divide, and can form various other types of cell. (
  • So far, there have been no reports in the literature of stem cell lines derived using an approach that does not require embryo destruction. (
  • Stem cells are taken from the inner cell mass of a five-day old blastocyst, destroying the developing embryo. (
  • Advanced Cell Technology ( is pioneering a new method in which stem cells are removed without destroying the embryo. (
  • The blastomere is cultured with an established embryonic stem cell line and subsequently separated to form new cell lines. (
  • 5) Remove these stem cells and culture them - - grow them in a Petri-dish, away from the controlling parent DNA, up to 10 million or more. (
  • 6) Growing these cells without the influence of the developing embryo will keep them at the stem cell stage. (
  • 7) Once the culture has grown to sufficient numbers, these stem cells can be used directly on the recipient. (
  • During the period of 1890 to 1904, systems for embryo rescue became systematic by applying nutrient solutions that contained salts and sugars and applying aseptic technique. (
  • The efficiency with which developing sunflower embryos convert substrates into seed storage reserves was determined by labeling embryos with [U-(14)C6]glucose or [U-(14)C5]glutamine and measuring their conversion to CO2, oil, protein and other biomass compounds. (
  • The average carbon conversion efficiency was 50%, which contrasts with a value of over 80% previously observed in Brassica napus embryos (Goffman et al. (
  • Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos. (
  • Efficiency of cloned embryo production could be improved further by propagating and standardizing this technique. (
  • The idea that you can start with a skin cell and make viable eggs in culture is quite amazing. (
  • Case reports of both fresh and cryopreserved embryo transfers have confirmed that day-7 blastocysts may still be viable [ 6 - 8 ]. (
  • While the disinfestation and explant excision processes differ for these three techniques, many of the factors that contribute to the successful recovery of viable plants are similar. (
  • Today, the quality of an embryo is assessed using microscopic evaluation of morphological parameters, but the method has limited predictive value. (
  • In the second experiment, embryos were recovered from sheep at the peak of viraemia. (
  • A total of 96 embryos were collected from BTV-infected sheep 21 days after infection. (
  • 181 cloned sheep embryos were produced in vitro by conventional SCNT and 92 by HMC. (
  • The embryos are then transplanted into female sheep and the lambs are born naturally. (
  • i) cartoon representing the Rabl configuration of chromosomes in the early embryo. (
  • At this time, PGD can analyze only a limited number of chromosomes and only a few centers worldwide have significant experience in this technique. (
  • In crosses between cultivated barley Hordeum vulgare 2x=14 with wild relative H. bulbosum , H. bulbosum chromosomes are usually lost in the developing embryo. (