Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
The sum of the weight of all the atoms in a molecule.
A highly miniaturized version of ELECTROPHORESIS performed in a microfluidic device.
Electrophoresis in which discontinuities in both the voltage and pH gradients are introduced by using buffers of different composition and pH in the different parts of the gel column. The term 'disc' was originally used as an abbreviation for 'discontinuous' referring to the buffers employed, and does not have anything to do with the shape of the separated zones.
Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium.
Electrophoresis applied to BLOOD PROTEINS.
Electrophoresis in which cellulose acetate is the diffusion medium.
Electrophoresis in which various denaturant gradients are used to induce nucleic acids to melt at various stages resulting in separation of molecules based on small sequence differences including SNPs. The denaturants used include heat, formamide, and urea.
Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The pH in solutions of proteins and related compounds at which the dipolar ions are at a maximum.
The rate dynamics in chemical or physical systems.
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.
An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Proteins found in any species of bacterium.
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
The protein complement of an organism coded for by its genome.
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A phenomenon in which the surface of a liquid where it contacts a solid is elevated or depressed, because of the relative attraction of the molecules of the liquid for each other and for those of the solid. (from McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Methods of comparing two or more samples on the same two-dimensional gel electrophoresis gel.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
The measurement of the density of a material by measuring the amount of light or radiation passing through (or absorbed by) the material.
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The application of molecular biology to the answering of epidemiological questions. The examination of patterns of changes in DNA to implicate particular carcinogens and the use of molecular markers to predict which individuals are at highest risk for a disease are common examples.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Established cell cultures that have the potential to propagate indefinitely.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Techniques for removal by adsorption and subsequent elution of a specific antibody or antigen using an immunosorbent containing the homologous antigen or antibody.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Techniques to partition various components of the cell into SUBCELLULAR FRACTIONS.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Sudden increase in the incidence of a disease. The concept includes EPIDEMICS and PANDEMICS.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Genotypic differences observed among individuals in a population.
The functional hereditary units of BACTERIA.
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
The relationships of groups of organisms as reflected by their genetic makeup.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Analogs of those substrates or compounds which bind naturally at the active sites of proteins, enzymes, antibodies, steroids, or physiological receptors. These analogs form a stable covalent bond at the binding site, thereby acting as inhibitors of the proteins or steroids.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)
A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Using MOLECULAR BIOLOGY techniques, such as DNA SEQUENCE ANALYSIS; PULSED-FIELD GEL ELECTROPHORESIS; and DNA FINGERPRINTING, to identify, classify, and compare organisms and their subtypes.
Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.
A group of compounds with the general formula M10(PO4)6(OH)2, where M is barium, strontium, or calcium. The compounds are the principal mineral in phosphorite deposits, biological tissue, human bones, and teeth. They are also used as an anticaking agent and polymer catalysts. (Grant & Hackh's Chemical Dictionary, 5th ed)
Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.
A series of steps taken in order to conduct research.
Proteins found in any species of virus.
Reagents with two reactive groups, usually at opposite ends of the molecule, that are capable of reacting with and thereby forming bridges between side chains of amino acids in proteins; the locations of naturally reactive areas within proteins can thereby be identified; may also be used for other macromolecules, like glycoproteins, nucleic acids, or other.
Transport proteins that carry specific substances in the blood or across cell membranes.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Substances that reduce the growth or reproduction of BACTERIA.
Antibodies produced by a single clone of cells.
A fractionated cell extract that maintains a biological function. A subcellular fraction isolated by ultracentrifugation or other separation techniques must first be isolated so that a process can be studied free from all of the complex side reactions that occur in a cell. The cell-free system is therefore widely used in cell biology. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p166)
Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
Chromatographic techniques in which the mobile phase is a liquid.
A proteolytic enzyme obtained from Streptomyces griseus.
The process of cleaving a chemical compound by the addition of a molecule of water.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Substances elaborated by bacteria that have antigenic activity.
RESTRICTION FRAGMENT LENGTH POLYMORPHISM analysis of rRNA genes that is used for differentiating between species or strains.
A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.
Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Proteins isolated from the outer membrane of Gram-negative bacteria.
The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.
Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
The chemical and physical integrity of a pharmaceutical product.
Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.
Any infection which a patient contracts in a health-care institution.
A sulfur-containing essential L-amino acid that is important in many body functions.
Techniques used to demonstrate or measure an immune response, and to identify or measure antigens using antibodies.
Elements of limited time intervals, contributing to particular results or situations.
Proteins prepared by recombinant DNA technology.
Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Deoxyribonucleic acid that makes up the genetic material of viruses.
Proteins in the cerebrospinal fluid, normally albumin and globulin present in the ratio of 8 to 1. Increases in protein levels are of diagnostic value in neurological diseases. (Brain and Bannister's Clinical Neurology, 7th ed, p221)
The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.
A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
Infections with bacteria of the genus STAPHYLOCOCCUS.
Abnormal immunoglobulins synthesized by atypical cells of the MONONUCLEAR PHAGOCYTE SYSTEM. Paraproteins containing only light chains lead to Bence Jones paraproteinemia, while the presence of only atypical heavy chains leads to heavy chain disease. Most of the paraproteins show themselves as an M-component (monoclonal gammopathy) in electrophoresis. Diclonal and polyclonal paraproteins are much less frequently encountered.
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
Direct nucleotide sequencing of gene fragments from multiple housekeeping genes for the purpose of phylogenetic analysis, organism identification, and typing of species, strain, serovar, or other distinguishable phylogenetic level.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
Sites on an antigen that interact with specific antibodies.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.
An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992)
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
Cyanogen bromide (CNBr). A compound used in molecular biology to digest some proteins and as a coupling reagent for phosphoroamidate or pyrophosphate internucleotide bonds in DNA duplexes.
Compounds containing the -SH radical.
Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Chemical agents that react with SH groups. This is a chemically diverse group that is used for a variety of purposes. Among these are enzyme inhibition, enzyme reactivation or protection, and labelling.
The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.

Hidden genetic variability within electromorphs in finite populations. (1/3912)

The amount of hidden genetic variability within electromorphs in finite populations is studied by using the infinite site model and stepwise mutation model simultaneously. A formula is developed for the bivariate probability generating function for the number of codon differences and the number of electromorph state differences between two randomly chosen cistrons. Using this formula, the distribution as well as the mean and variance of the number of codon differences between two identical or nonidentical electromorphs are studied. The distribution of the number of codon differences between two randomly chosen identical electromorphs is similar to the geometric distribution but more leptokurtic. Studies are also made on the number of codon differences between two electromorphs chosen at random one from each of two populations which have been separated for an arbitrary number of generations. It is shown that the amount of hidden genetic variability is very large if the product of effective population size and mutation rate is large.  (+info)

Application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria. (2/3912)

Strains belonging to the Gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (TGGE). This method allows the sequence-specific separation of amplified fragments of 16S rRNA genes. A total of 115 reference strains representing the majority of the species of the genera Aeromicrobium, Agromyces, Arthrobacter, Aureobacterium, Cellulomonas, Curtobacterium, Nocardioides and Terrabacter were characterized. Depending on the genus investigated, the resolution limit of the technique appeared to be at the species or genus level or intermediate between the two. Aberrant TGGE profiles of strains within particular taxa revealed genomic heterogeneity and generic misclassification of nine strains studied. Beyond that, indications of 16S rRNA gene heterogeneity were found within the genomes of three Curtobacterium strains. The misclassifications revealed by TGGE were confirmed using whole-cell fatty acid methyl ester analysis and subsequent comparison with a database. TGGE has been demonstrated to be a useful tool in bacterial taxonomy.  (+info)

Evidence for suppressed activity of the transcription factor NFAT1 at its proximal binding element P0 in the IL-4 promoter associated with enhanced IL-4 gene transcription in T cells of atopic patients. (3/3912)

Allergen-specific T cells in atopic patients are polarized IL-4-producing Th2 cells, promoting IgE synthesis by B cells. The molecular basis for increased IL-4 gene expression in atopy is not fully understood. IL-4 gene regulation in general involves the nuclear factor of activated T cells (NFAT) family of transcription factors, of which NFAT1 and NFAT2 are most prominent in peripheral T cells. Recently, a unique inhibitory role of NFAT1 in IL-4 gene control was shown in the mouse. In a series of electrophoretic mobility shift assays with protein extracts of highly polarized Th2 clones from atopics and Th1 clones from controls we compared DNA-binding activities at the two NFAT-binding elements P0 and P1 of the crucial proximal human IL-4 promoter. At the most proximal P0 site, NFAT-containing complexes devoid of NFAT2 were readily inducible in the Th1 clones, but hardly or not in the Th2 clones. In contrast, both in Th1 and Th2 clones NFAT-containing complexes were strongly inducible at the P1 site, consisting of NFAT2 and a P0-compatible NFAT activity, without apparent differences between Th1 and Th2 clones. Like in Th2 clones, suppressed NFAT-P0 complex formation was observed also at the polyclonal level in peripheral blood mononuclear cells (PBMC) of three of five severe atopic dermatitis patients with strongly elevated serum IgE levels, but not in control PBMC. These findings suggest that high-level IL-4 production in atopic Th2 cells is associated with selective reduction of suppressive NFAT1 activity at the IL-4 P0 element and that some patients with this multifactorial disease may have a putative systemic disorder at this level.  (+info)

Hepatocyte nuclear factor-4 regulates intestinal expression of the guanylin/heat-stable toxin receptor. (4/3912)

We have investigated the regulation of gene transcription in the intestine using the guanylyl cyclase C (GCC) gene as a model. GCC is expressed in crypts and villi in the small intestine and in crypts and surface epithelium of the colon. DNase I footprint, electrophoretic mobility shift assay (EMSA), transient transfection assays, and mutagenesis experiments demonstrated that GCC transcription is regulated by a critical hepatocyte nuclear factor-4 (HNF-4) binding site between bp -46 and -29 and that bp -38 to -36 were essential for binding. Binding of HNF-4 to the GCC promoter was confirmed by competition EMSA and by supershift EMSA. In Caco-2 and T84 cells, which express both GCC and HNF-4, the activity of GCC promoter and/or luciferase reporter plasmids containing 128 or 1973 bp of 5'-flanking sequence was dependent on the HNF-4 binding site in the proximal promoter. In COLO-DM cells, which express neither GCC nor HNF-4, cotransfection of GCC promoter/luciferase reporter plasmids with an HNF-4 expression vector resulted in 23-fold stimulation of the GCC promoter. Mutation of the HNF-4 binding site abolished this transactivation. Transfection of COLO-DM cells with the HNF-4 expression vector stimulated transcription of the endogenous GCC gene as well. These results indicate that HNF-4 is a key regulator of GCC expression in the intestine.  (+info)

Nuclear factor-kappa B activity in T cells from patients with rheumatic diseases: a preliminary report. (5/3912)

OBJECTIVE: The NF-kappa B/Rel family of transcription factors regulates the expression of many genes involved in the immune or inflammatory response at the transcriptional level. The aim of this study was to determine whether distinctive patterns of NF-kappa B activation are seen in different forms of joint disease. METHODS: The DNA binding activity of these nucleoproteins was examined in purified synovial and peripheral T cells from patients with various chronic rheumatic diseases (12: four with rheumatoid arthritis; five with spondyloarthropathies; and three with osteoarthritis). RESULTS: Electrophoretic mobility shift assays disclosed two specific complexes bound to a NF-kappa B specific 32P-labelled oligonucleotide in nucleoproteins extracted from purified T cells isolated from synovial fluid and peripheral blood of patients with rheumatoid arthritis. The complexes consisted of p50/p50 homodimers and p50/p65 heterodimers. Increased NF-kappa B binding to DNA in synovial T cells was observed relative to peripheral T cells. In non-rheumatoid arthritis, binding of NF-kappa B in synovial T cells was exclusively mediated by p50/p50 homodimers. CONCLUSION: Overall, the results suggest that NF-kappa B may play a central part in the activation of infiltrating T cells in chronic rheumatoid arthritis. The activation of this nuclear factor is qualitatively different in rheumatoid synovial T cells to that in other forms of non-rheumatoid arthritis (for example, osteoarthritis, spondyloarthropathies).  (+info)

"The FSGS factor:" enrichment and in vivo effect of activity from focal segmental glomerulosclerosis plasma. (6/3912)

A circulating causative factor has been postulated in focal segmental glomerulosclerosis (FSGS). It has been shown that serum or plasma from some FSGS increases glomerular albumin permeability (Palb) in vitro. Palb greater than 0.5 (i.e., FS activity) is associated with recurrence after transplantation. Specimens from 15 FSGS patients were studied to document the presence of a permeability factor, to isolate this factor, to characterize its biochemical properties, and to show its effect in vivo. Total lipids were extracted by chloroform/methanol (2: 1); FS activity was absent from total lipid extract. Chylomicrons and lipoproteins were removed from the plasma with dextran sulfate, followed by sequential precipitation of proteins at 50 and 70% ammonium sulfate saturation. FS activity was retained in the 70% ammonium sulfate supernatant and exhibited a 100-fold purification. FS activity was lost after heating at 100 degrees C for 10 min or after protease digestion. Under nondenaturing conditions, electrophoresis of the FSGS 70% supernatant showed a prominent low molecular weight band that was not evident in the 70% supernatant from normal plasma. Dialysis and centrifugation-based membrane ultrafiltration of the FSGS factor indicated a molecular size between 30 and 50 kD. Injection of the 70% FSGS supernatant into rats caused a threefold increase in urine protein in collections from 6 to 24 h after injection. No increase in proteinuria occurred in rats injected with 70% supernatant from normal individuals. It is concluded that the FSGS factor is a low molecular weight protein with the potential to increase Palb in vitro and to cause proteinuria in vivo.  (+info)

Isolation of DNA fragments associated with methylated CpG islands in human adenocarcinomas of the lung using a methylated DNA binding column and denaturing gradient gel electrophoresis. (7/3912)

We have constructed a library of DNA fragments heavily methylated in human adenocarcinomas of the lung to permit the comprehensive isolation of methylated CpG islands in cancer. Heavily methylated genomic DNA fragments from tumors of nine male patients were enriched using a methylated DNA binding column and used for construction of the library. From this library, DNA fragments having properties of CpG islands were isolated on the basis of their reduced rate of strand dissociation during denaturing gradient gel electrophoresis. Approximately 1,000 clones, corresponding to 0.3% of the library were analyzed, and nine DNA fragments were identified as being associated with CpG islands that were methylated in tumor DNA. One CpG island was methylated specifically in tumor DNA, whereas the remaining eight CpG islands were methylated both in normal and tumor DNA derived from the same patients. Our results suggest that the number of CpG islands methylated specifically in tumors is not large. The library, which contains DNA fragments from methylated CpG islands comprehensively, is expected to be valuable when elucidating epigenetic processes involved in carcinogenesis.  (+info)

An electrophoretic study of the reversible binding of phosphate to ovalbumin. (8/3912)

An electrophoretic method is described for the measurement of relatively weak interaction of ions with proteins, and illustrated with the ovalbumin-phosphate system in 0.1I buffers, pH6.1. Results indicate that under these conditions the interaction of a single dibasic phosphate ion with ovalbumin is described by an association constant of approx. 250M-1.  (+info)

A continuous forced flow electrophoresis cell and a method of operation for the fractionation of an aqueous liquid, such as blood, containing at least two compounds, the relative mobilities of which in an electric field vary as a function of the pH, in order to obtain one fraction enriched and one depleted in one of the compounds, the cell having six compartments divided by ion permeable membranes, the end cell containing an anode and cathode respectively, the central cells being separated by a microporous membrane. The liquid is fed to one of the central cells the filtered fraction being removed, after passage through the microporous membrane, from the other. A main electrolyte is fed to and from the end cells and an auxiliary electrolyte to the intermediate cells such that the pH in one intermediate cell differs from that of the other.
A method for the isolation of brush-border membranes from newborn-rat kidney, employing centrifugation and free-flow electrophoresis, is described. The composition and purity of the preparation was assessed by determination of enzyme activities specific for various cellular membranes. Free-flow electrophoresis resolves the newborn-rat renal membrane suspension into two populations of alkaline phosphatase-enriched brush-border membranes, designated A and B, with the A peak also showing activity of (Na+ + K+)-stimulated ATPase, the basolateral membrane marker enzyme, whereas those of the B peak were enriched 11-fold in alkaline phosphatase and substantially decreased in (Na+ + K+)-stimulated ATPase activity. Membranes in the A peak showed a 7-fold enrichment of alkaline phosphatase, and (Na+ + K+)-stimulated ATPase activity similar to that of the original homogenate. Proline uptake employed to assess osmotic dependency revealed 7% binding of proline to the B vesicles and 31% to the A vesicles. ...
Fig 1. Principle of free-flow electrophoresis. The miniaturization of FFE implies several advantages especially considering sample volume and separation speed. In contrast to the tens of milliliters of sample consumed by conventional large scale FFE devices, microfluidic FFE systems require only tens of nanoliters up to hundreds of microliters of sample. This is especially interesting in clinical analysis where often only low sample volumes are available. Furthermore, instead of residence times of up to tens of minutes, microfluidic FFE (µ-FFE) devices separate within several seconds. The aim of this project is to develop a microfluidic free-flow electrophoresis device (as shown in figure 2 and 3) which eventually might find application in so called proteomics-on-a-chip systems for proteome analysis.. ...
Temperature-gradient gel electrophoresis (TGGE) was employed to determine the thermal stabilities of 48 DNA fragments that differ by single base pair mismatches. The approach provides a rapid way for studying how specific base mismatches effect the stability of a long DNA fragment. Homologous 373 bp DNA fragments differing by single base pair substitutions in their first melting domain were employed. Heteroduplexes were formed by melting and reannealing pairs of DNAs, one of which was ³²P-labeled on its 5-end. Product DNAs were separated based on their thermal stability by parallel and perpendicular temperature-gradient gel electrophoresis. The order of stability was determined for all common base pairs and mismatched bases in four different nearest neighbor environments; d(GXT) d(AYC), d(GXG) d(CYC), d(CXA) d(TYG), and d(TXT) * d(AYA) with X,Y = A,T,C, or G. DNA fragments containing a single mismatch were destabilized by 1 to 5°C with respect to homologous DNAs with complete Watson - Crick ...
FFE Service GmbH offers the following products: free flow electrophoresis system, an upgrade kit for existing instruments and a FFE robotic system.
FFE Service GmbH conducts Free Flow Electrophoresis training courses, where customers can bring their own sample and elaborate the optimum protocols during the training.
article{5aecc78e-10a8-4a8d-a992-f32f2a699c72, abstract = {Microchip-based free flow acoustophoresis (FFA) in combination with two-dimensional cell prefocusing enables concurrent multiple target outlet fractionation of leukocytes into subpopulations (lymphocytes, monocytes, and granulocytes); we report on this method here. We also observed significantly increased accuracy in size-based fractionation of microbeads as compared to previously presented FFA multiple outlet systems. Fluorescence microscopy illustrates the importance of two-dimensional prefocusing where a sample mixture of 3, 7, and 10 mu m beads are separated into well-confined particle streams and collected in their respective target outlets. Flow cytometry data for lymphocytes and granulocytes, respectively, in their corresponding outlets verify concurrent isolation of leukocyte subpopulations with high purity (95.2 +/- 0.6% and 98.5 +/- 0.7%) and high recovery (86.5 +/- 10.9% and 68.4 +/- 10.6%). A relatively low purity and high ...
May 2, 2007. S. 1267 (110th). A bill to maintain the free flow of information to the public by providing conditions for the federally compelled disclosure of information by certain persons connected with the news media. In GovTrack.us, a database of bills in the U.S. Congress.
Buy Selle Italia SLR Friction Free Flow Titanium Saddle at Wheelbase, UKs largest cycle store. Bicycle mail order specialists. FREE shipping. 0% FINANCE. For sales and advice call 01539 821443
Free Flow IPA is a American IPA style beer brewed by Otter Creek Brewing in Middlebury, VT. 4.08 average with 268 ratings, reviews and opinions.
Although the phenomenon we now call dielectrophoresis was described in passing as far back as the early 20th century, it was only subject to serious study, named and first understood by Herbert Pohl in the 1950s.[9][10] Recently, dielectrophoresis has been revived due to its potential in the manipulation of microparticles,[2][4][5][11]nanoparticles and cells. Dielectrophoresis occurs when a polarizable particle is suspended in a non-uniform electric field. The electric field polarizes the particle, and the poles then experience a force along the field lines, which can be either attractive or repulsive according to the orientation on the dipole. Since the field is non-uniform, the pole experiencing the greatest electric field will dominate over the other, and the particle will move. The orientation of the dipole is dependent on the relative polarizability of the particle and medium, in accordance with Maxwell-Wagner-Sillars polarization. Since the direction of the force is dependent on field ...
Microreactors have gained increasing attention in their application toward continuous micro flow synthesis. An unsolved problem of continuous flow synthesis is the lack of techniques for continuous...
This thesis presents two new concepts for separation of micro particles using dielectrophoresis, demonstrated by calculated examples, as well as a new method for obtaining dielectric data on living cells. The thesis is based on four papers.. Paper I describes how the trapping efficiency of micro particles may be significantly increased when superpositioned electric fields are employed in a high conductivity medium. Avoiding low conductivity media is important when working with living cells. Calculations were performed to predict trajectories of Escherichia coli bacteria in the system with superpositioned electric fields, and a model was developed which employed two arrays of interdigitated electrodes in a micro channel.. Paper II proposes a new concept for separation of micro particles, based on repetitive dielectrophoretic trapping and release in a flow system. Calculations show that the resolution increases as a direct function of the number of trap and release steps, and that a difference in ...
Vital Baby Soft Spout Babys 1st Tumbler - a 120ml cup with a soft spout. Perfectly sized for little hands and can be used either non-spill or free flow as your baby develops. Simply remove the 2-in1 non-spill valve from the cap, and the cup can be used as a free-flow cup. With the value in place, liquid will only flow when your baby drinks from the soft spout. Vital Baby cups have soft spouts, and grips that are kind and gentle to your babys sensitive mouth and tiny hands, allowing you to choose the cup that best suits your babys changing needs as it develops. Age range from 4+ months. All Vital Baby cups are dishwasher safe if placed on the top shelf and can be steam sterilised. We really have thought of everything! 1. Both non-spill and free flow drinking 2. Protect your babys sensitive gums with our soft spout 3. Built-in measurement markings to monitor how much liquid baby is drinking 4. Cleverly designed hygienic spout cover 5. 120ml/4oz capacity 6. BPA-Free
Our research takes advantage of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE), a technology that we have extensively modified over the years to give us a suite of methods for comprehensive analysis of glycosylation processes of the ER. These innovations have led to many exciting collaborations with investigators both at UT Southwestern and around the world, involving biological samples from various animals, parasites, plants, and bacteria. Here is a partial list of labs that have recently collaborated with us to use FACE, with PubMed links to Resulting publication that resulted:. ...
That episode was so over the top gross that it is leading me to believe that Shonda is going for the shock factor and no longer confident that her characters stories are strong enough to keep us coming back. All, I know is that I couldnt get through that episode fast enough and the case of that poor man has stayed with me even today. I feel nauseous about it and this is not how I want to feel. If I want to know about the health issues of the indigent people of third world countries, Ill go to National Geographic ...
Tong Ze Bu Tong; Bu Tong Ze Tong Huang Di Nei Jing If there is free flow, there is no pain; if there is pain, there is lack of free flow. In Traditional Chinese Medicine, Qi (air or fluid) is stimulated at specific Acupuncture points to create a balanced flow throughout the body, this is said to balance the Yin and Yang of the body and create a smooth flow of [...]. ...
A method of simultaneously forming a multiplicity of surface mountable solid state capacitors is disclosed. The method comprises mounting on a substrate a wafer of powdered solid state capacitor forming metal, sintering the wafer and metal together to fuse the interface between wafer and substrate to each other and convert the wafer into a porous integral mass, dividing the sintered wafer into a multiplicity of mutually spaced sub-units, isolating the interface or boundary between the substrate and sub-units one from the other by a resin infusion or by a dielectric deposition step, causing the sub-units to be converted to capacitors by sequential anodizing and manganizing steps, bonding a cathode plate to the counter electrode components present on the upper surfaces of the sub-units in electrical contact therewith, filling the voids between adjacent sub-units with insulative resin material by injecting same between the plates utilizing the plates as elements of a mold, and after hardening of the resin
Medox-Bio electrophoretic systems are designed to suit various applications related to Nucleic acid separation, Protein seperation, Immuno protein separation and Protein transfer in laboratories. These computer designed systems are made out of well-polished and clear Plexiglass sheets fused together by using high-strength specific adhesives.
An electrophoretic analysis of the dissolved precipitate and supernatant fluid was conducted in the equivalent zone. For microelectrophoresis on paper, a Flynn and De Mayo type of chamber as modified...
Although bacterial culture remains the gold standard for detection of viable bacteria in environmental specimens, the typical time requirement of twenty-four hours can delay and even jeopardize appropriate determination. In addition, culture is incapable of detecting viable but not culturable (VBNC) species. Conversely, nucleic acid and antibody-based methods decrease time to detection but rarely characterize viability of the bacteria detected. Through selection by membrane permeability, the method described in this work employs positive dielectrophoresis (pDEP) for separation and purification of viable and VBNC species from water and allows concentration of bacteria for downstream applications.
Nucleotides are the sub-units that make up DNA, and are made up of even smaller sub-units. The differences between these sub-units result in the unique base pairs that record genetic information and make up genes. More » ...
Electrophoretic variation of proteins encoded by 14 loci was analyzed in eight (five continental and three insular) populations of the Chilean leptodactylid frog Batrachyla leptopus.
Tumor initiating cells (TICs), also known as cancer stem cells, are a finite population of cancer cells that have the ability to transplant a new tumor from an...
Sigma-Aldrich offers abstracts and full-text articles by [Rebecca Soffe, Shi-Yang Tang, Sara Baratchi, Sofia Nahavandi, Mahyar Nasabi, Jonathan M Cooper, Arnan Mitchell, Khashayar Khoshmanesh].
A device for holding an IV tube in a pumping apparatus and for selectively occluding the tube when the door of the pumping apparatus is opened, to prevent free flow of fluid in the tube. The device consists of a base member to which the IV tube is attached or through which it passes, and a slide clamp which slides relative to the base member and relative to the tube. The IV tube is passed through an elongated aperture in the slide clamp, and the slide clamp is slidingly mounted on the base member. The aperture in the slide clamp has a relatively open hole at one end, through which the tube passes when the slide clamp is in one position, allowing fluid to flow through the tube. The aperture also has a relatively constricted slot at the other end, through which the tube passes when the slide clamp is in a second position, preventing fluid flow through the tube. A flexible cantilevered arm on the base interlocks with a locking projection on the slide clamp to lock the slide clamp in the second
Body plan: molds Hyphae - long filaments that invade their food source and provide a large surface area to absorb nutrients Mycelium - a tangled mat of hyphae Some have cells separated by cell walls called septa, others do not - coenocytic Even those with septa often have large pores that allow free flow of organelles between cells
Build confidence as your toddler grows and needs larger amounts of liquid. Its soft spout, easy grip base and large 280ml capacity make our Toddler Trainer Cup perfect for this. It can be used as non spill or free flow. Our award winning range of Vital Baby® Trainer Cups is specially designed to protect sensitive gums, teach how to hold, build confidence and ultimately help to develop baby s drinking skills as they grow. Every cup comes complete with a non-spill valve which has been cleverly designed to provide a smooth drinking action and a steady flow of liquid from just 4 months old!
Earth - Spleen-Pancreas/Stomach - is associated with the harvest time. It is the patient and nourishing mediator, it represents balance and neutrality. The colour is yellow, the flavour bland or sweet and the negative emotions are worry and over thinking; positive is fairness and openness - and singing is associated with the free flow of spleen Chi. You can learn more by watching the following short video. The Five Phases correspond to each other through nourishing and controlling cycles. Metal nourishes Water, Water nourishes Wood, Wood nourishes Fire, Fire nourishes Earth, Earth nourishes Metal ...
Talk of such policies is often met by objections that this will lead to a dangerous balkanisation of global capital markets, preventing the free flow of capital and stymieing its allocative efficiency benefits, he said. But since the evidence for the benefits of financial integration is at best elusive and ambiguous, some balkanisation of short term international debt markets could be a good thing ...
Economic and trade sanctions enacted by one government against another often have detrimental effects on the free flow of digital communications and communications technologies that activists, innovators and ordinary users of technologies desperately need. EFF has a long history of fighting against those effects, especially by the US government. Since the beginning, the
WHAT CAUSES SNORING? The noisy sounds of snoring occur when there is an obstruction to the free flow of air through the passages at the back of the mouth
This report is chilling in its implications on the free flow of information to the American people. Under the bill, which is written in extremely broa
Creating urban spaces that allow for the free flow and penetration of water and wind is essential to the survival of water-based cities like Bangkok.
Snoring is most annoying health situation, snoring, it is a noise reason when there is an obstacle to the free flow of air through the passages at the back of the mouth and nose. That area is the collapsible part of the airway. Snoring happens when these structures hit each other and vibrate during breathing.. ...
Hubby saya memang dah nak try makan kat restoran ni masa dia datang ke JB sebelum tu tapi tak berani dan tak best sorang2...sbb dia menyediakan hidangan steamboat dan BBQ secara buffet. Alhamdulillah amat puasa hati makan kat sini. Pilihannya amatlah banyak berpuluh2 jenis rasanya...macam2 ada,ayam lebih 10 jenis, daging lembu, daging kambing dan ikan ,pelbagai jenis fishballs, meatballs, udang, sotong, kerang, mussels, sayur, dessert (aiskrim, ABC, cendol, buah-buahan etc) dan air yang free flow (soft drink, juice). Harga RM70++ untuk dua orang ...
Advanced search is divided into two main parts, and one or more groups in each of the main parts. The main parts are the Search for (including) and the Remove from search (excluding) part. (The excluding part might not be visible until you hit NOT for the first time ...
Definition : Tubes covered with supporting media that are used to separate an ionic mixture of solutes into its components by the differences in migration rate when an electric field is applied (electrophoresis). These columns are usually made of glass or plastic (e.g., acrylic) tubes; they are usually mounted vertically, with both ends placed into buffer wells that make the electrical connection between the columns and the electrodes. Typically, several columns are run simultaneously in an electrophoretic system.. Entry Terms : Columns, Electrophoresis , Immunoelectrophoresis Equipment , Apparatus, Electrophoresis , Electrophoresis Equipment , Chromatography Equipment. UMDC code : 18551 ...
Mulhall, HJ, Abdallat, R, Liang, X, Fedele, S, Lewis, MP, Porter, S, Tsinkalovsky, O, Johannessen, AC, Hughes, MP, Costea, DE and Labeed, FH (2010) Rapid detection of oral cancer: Electrophysiological characterization by dielectrophoresis technology In: Nanotech Conference & Expo 2010, 2010-06-21 - 2010-06-24, Anaheim, USA. Armellin, R, Lavagna, M, Starkey, RP and Lewis, MJ (2006) Aero-gravity assist maneuvers: Coupled trajectory and vehicle shape optimization In: AIAA/AAS Astrodynamics Specialist Conference and Exhibit, 2006-08-21 - 2006-08-24, Keystone, U.S.A.. ...
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Summary: A 16S rRNA-based molecular ecological study was performed to search for dominant bacterial sequences in Drentse A grassland soils (The Netherlands). In the first step, a library of 165 clones was generated from PCR-amplified 16S rDNA. By sequence comparison, clone DA079 and two other identical clones could be affiliated to a group of recently described uncultured Actinobacteria. This group contained 16S rDNA clone sequences obtained from different environments across the world. To determine whether such uncultured organisms were part of the physiologically active population in the soil, ribosomes were isolated from the environment and 16S rRNA was partially amplified via RT-PCR using conserved primers for members of the domain Bacteria. Subsequent sequence-specific separation by temperature-gradient gel electrophoresis (TGGE) generated fingerprints of the amplicons. Such community fingerprints were compared with the TGGE pattern of PCR-amplified rDNA of clone DA079 which was generated with the
Article: Smith, James P.; Huang, Chao; Kirby, Brian J.; (2015) Enhancing Sensitivity and Specificity in Rare Cell Capture Microdevices with Dielectrophoresis, Biomicrofluidics, 9(1). DOI. Abstract: The capture and subsequent analysis of rare cells, such as circulating tumor cells from a peripheral blood sample, has the potential to advance our understanding and treatment of a wide range of diseases. There is a particular need for high purity (i.e., high specificity) techniques to isolate these cells, reducing the time and cost required for single-cell genetic analyses by decreasing the number of contaminating cells analyzed. Previous work has shown that antibody-based immunocapture can be combined with dielectrophoresis (DEP) to differentially isolate cancer cells from leukocytes in a characterization device. Here, we build on that work by developing numerical simulations that identify microfluidic obstacle array geometries where DEP-immunocapture can be used to maximize the capture of target ...
Cavalli, sforza L.; Santachiara, S A.; Wang, L; Erdelyi, E; and Barchas, J, Electrophoretic study of 5-hydroxytryptophan decarboxylase from brain and liver in several species. (1974). Subject Strain Bibliography 1974. 607 ...
TY - GEN. T1 - Dielectrophoresis of DNA using 3D Carbon Electrodes. T2 - Electrophoresis. AU - Martinez-Duarte, R. AU - Camacho-Alanis, F. AU - Renaud, P. AU - Ros, A. PY - 2013. Y1 - 2013. M3 - Other contribution. ER - ...
Mote B. Normal serum protein values in newborn infants, determined by cellulose acetate electrophoresis. J Am Osteopath Assoc 1967;67(4):430. doi: https://doi.org/.. Download citation file:. ...
Carbon nanomaterials have a large surface area and complex particles shape architecture. These qualities are successfully applying to develop new drug carriers, fillers, or nanodetectors [1]. At the same time, the utilization efficiency of such materials is caused by maintaining of the nanodispersity. An agglomeration and partially sedimentation of nanotubes in aqueous media is a well-known fact. The stability of the nanotube dispersion mostly depends on the uniformity of the boundary layer and nanotube morphology. The hydrophobic nature of defectless areas of their surface and the hydrophilicity of some types of defects in the nanotube boundary layer, namely carboxy- or phenolic groups [2, 3] which are formed during nanotube purification and washing, are competing during dispersing in the aqueous media. An increase in stability of the nanotube dispersion in water can be achieved through the surface charge via formation of the double electrical layer (EDL). Among other carbon materials, carbon ...
Fluid-Screen, Inc. presents a bacterial concentration and filtration method based on dielectrophoresis (DEP). DEP has been known to induce particle motion since the 1960s. However, yields and reproducibility have consistently been low in practice, reducing the potential of DEP for practical applications. Here we present a novel electrode design to induce high electric field gradients. This design allows rapid and efficient capture of diverse bacteria from aqueous solutions of varied conductivity. By using the novel spiral electrode design and tuning the electric field to species-specific frequencies, we show capture of over 99% of bacteria within seconds of gradient application. Our method of DEP induces responses in both the representative Gram-negative Escherichia coli and Gram-positive Enterococcus faecalis bacteria. Equilibrium repositioning of suspended bacteria can be seen within miliseconds for low-conductivity medium and within seconds for higher conductivity solutions such as environmental
Development and Validation of a Simple and Rapid Capillary Zone Electrophoresis Method for Quantification of Heparin in a Pharmaceutical Product and Stability Studies Abstract.
Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and ...
If you want to hydrate and moisturize your skin instantly, then Freshen Up Facial Spray is a great toner to be in your makeup kit, as it is blended with Rosewater Hydrosol!. Your skin maintains proper oil balance (pH) but gets disturbed with outside forces that act on it in the form of alkaline soaps or atmospheric impurities. Freshen Up maintains a constant pH balance and also contains astringent properties that combat the acne developing bacteria and reduces skin defects. It is a Rosewater Hydrosol that helps in hydrating your skin in the most natural way. Can be used for all the skin types as the formulation is absolutely skin-safe! Use it once, after your bath and keep your skin healthy through the day.. Results have shown that Freshen Up Facial Spray can prevent ingrown hair thus aiding hair tidiness! 8 oz.. ...
The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transitted through the chamber, it exists at the opposite end of the chamber at a characteristic position. Methods according to
The present disclosure is directed to a novel apparatus and novel methods for the separation, characterization, and manipulation of matter. In particular, the invention combines the use of frequency-dependent dielectric and conductive properties of particulate matter and solubilized matter with the properties of the suspending and transporting medium to discriminate and separate such matter. The apparatus includes a chamber having at least one electrode element and at least one inlet and one output port into which cells are introduced and removed from the chamber. Matter carried through the chamber in a fluid stream is then displaced within the fluid by a dielectrophoretic (DEP) force caused by the energized electrode. Following displacement within the fluid, matter travels through the chamber at velocities according to the velocity profile of the chamber. After the matter has transmitted through the chamber, it exits at the opposite end of the chamber at a characteristic position. Methods according to
URN zum Zitieren dieses Dokuments: urn:nbn:de:bvb:355-epub-219889. Hansen, Ernil und Hannig, Kurt (1984) Electrophoretic separation of lymphoid cells. Methods in enzymology 108, S. 180-197 ...
In order to point out the driven forces in the loading and release of protein onto thermally sensitive poly(N-isopropylacrylamide) (NIPAM) nanogel particles, which largely claimed in drug delivery and analytical processes, various polyNIPAM based nanogel particles were prepared, and the loading and release of two model proteins: bovine serum albumin (BSA) and -Globulins (G), onto various cationically or anionically charged polyNIPAM nanocrogel particles were investigated. At first, the nanogels particles were well characterized regarding their colloidal properties (swelling ability, particle size, and electrokinetic properties). Then, the loading and release of proteins behaviour onto the elaborated particles were investigated as a function of temperature, pH, and salinity. It was found that the loaded amount of both proteins increases as a function of temperature irrespective of the nature of charge of polyNIPAM nanogels. The protein loaded amount onto the two lower charged polyNIPAM ...
This thesis presents two new concepts for separation of micro particles using dielectrophoresis, demonstrated by calculated examples, as well as a new method for obtaining dielectric data on living cells. The thesis is based on four papers.. Paper I describes how the trapping efficiency of micro particles may be significantly increased when superpositioned electric fields are employed in a high conductivity medium. Avoiding low conductivity media is important when working with living cells. Calculations were performed to predict trajectories of Escherichia coli bacteria in the system with superpositioned electric fields, and a model was developed which employed two arrays of interdigitated electrodes in a micro channel.. Paper II proposes a new concept for separation of micro particles, based on repetitive dielectrophoretic trapping and release in a flow system. Calculations show that the resolution increases as a direct function of the number of trap and release steps, and that a difference in ...
An activated carbon fiber composite for separation and purification, or catalytic processing of fluids is described. The activated composite comprises carbon fibers rigidly bonded to form an open, permeable, rigid monolith capable of being formed to near-net-shape. Separation and purification of gases are effected by means of a controlled pore structure that is developed in the carbon fibers contained in the composite. The open, permeable structure allows the free flow of gases through the monolith accompanied by high rates of adsorption. By modification of the pore structure and bulk density the composite can be rendered suitable for applications such as gas storage, catalysis, and liquid phase processing.
This car is a ground effects machine and rides on a five inch cushion of air and can run on land or water, has no wheels, transmission or rear end. Plus, no frictional moving parts. Air is drawn in through the front and rear openings into the open plenum chamber with outlets through a combination of peripheral jet inserts. Four inch polyethylene circular skirt is installed on the lower portion of the body. Frame construction is of 3/4 inch alloy round tubing and the dimensions are 12′ long, 6′ wide, and 30″ high. Stabilizer fins has an 8 7 wingspan, plus 53 high. All panels are hand formed from 040 half hard aluminum, by Jack Sutton. Power is two jet aircraft starter motors 24 volt D.C., 4 horse power that turns 40,000 rpm at 300 amps. This is reduced 8 to 1 to absorb the amount of load combination of horsepower. Balanced 20 inches cast aluminum fans push 11,800 C.I.M. free flow & under ₖ″ static water pressure has 7,600 C.I.M., dry weight of the car is 422 pounds including all the ...
A recent WHO review of the PIP Framework offered an opportunity to close major gaps but also revealed the texts rigidity in adapting to rapid scientific progress (10). GSD fundamentally change the paradigm within which the PIP Framework operates, potentially rendering the framework less effective.. In the short run, it would be best simply to interpret the framework to include GSD as biological materials. Keeping GSD under the umbrella of a global agreement would facilitate tracking sequencing data, while holding users accountable-facilitating socially productive research, while limiting harmful use. A tracking system need not be burdensome or restrict the free flow of scientific information. A simple registration system that monitors who has downloaded GSD would help ensure that recipients abide by global research norms and could be incorporated into existing databases such as the GISAID platform. Moreover, allowing free exchange of GSD without enforcing the obligations owed to the donating ...
A BMW is a unique experience all in its own, but a tuned BMW makes it truly unique. This 2016 Alpine White M3 was not only ordered completely optioned out but was also ordered with the Dinan S1 Signature Package. Modifications of the package include a Stage 2 Upgrade, Dinan Free Flow Stainless Exhaust, Carbon Fiber Cold Air Intake, High Performance Adjustable Coil-Over Suspension System, Sway Bars, Pedal Cover Set, and of course an under the hood plaque and deck lid badge for proof of the modifications. Other than the Dinan labeled black exhaust tips, this M3 gives fairly stock appearance with its Carbon Fiber Diffuser and by not flaunting any badge on the trunk other than the Black M Badge.. Being as powerful a vehicle as the M3 is, making sure it can stop properly is important. Stopping isnt a problem for this M3 with its M Carbon Ceramic Brakes and Yellow Calipers that not only prove it, but also add on to the clean appearance of the car. Keeping the black badging and trimming consistent, ...
Did you know that private companies can patent genes in your body? A company called Myriad Genetics owns two genes known as BRCA 1 and BRCA2 -or Breast Cancer 1 and Breast Cancer 2. Women with certain mutations in these genes have a strong chance of getting breast or ovarian cancer.. This has very dangerous implications for womens health and scientific research.. The government should not be granting private entities control over something as personal and basic to the human body as our genes. Moreover, granting patents that limit scientific research, learning and the free flow of information violates the First Amendment.. ...
Plaque, a combination of cholesterol, fat, and other substances starts to stick to the walls lining your blood vessels which builds up over time. This makes arteries become harder and narrower,resulting in atherosclerosis.These plaques can break or rupture, by which blood cells called platelets will try to repair the artery, forming a blood clot. This buildup prevents a free flow of blood through the arteries. Blood carries oxygen and nutrients to the heart. If you dont get enough, it can lead to shortness of breath and chest pain (angina).. ...
But making hydrogen requires energy too. Making nitrogen fertilizers uses lots of hydrogen, so those serious about fuel supplies will also have to worry about food supplies. I just wish the same people who talk about shutting down industries and exacting revenge would instead busy themselves with showing that alternatives can be successfully commercialized, because if they cant theres little sense in using them. Energy independence will not come from government investing in unworkable alternatives. Energy independence can only come from the use and exploitation of domestic energy sources and this includes coal. Oil and related hydrocarbons are intrinsically cheap. If we decide to move away from them because we dont like or cant shoulder the associated problems, e.g., keeping the free flow free, wed better prepare for somebody else doing the dirty jobs. And to the winner goes the spoils ...
Snoring causes a person to wake up throughout the night. For most, snoring is simply a case of having to move your body from a bad position into one thats more comfortable to breathe in. But for the 18 million people in the United States suffering from OSA, snoring is their bodys cry for help. Throughout the night people with OSA literally stop breathing. They stop breathing because the muscles in their mouth, jaw, and throat relax, blocking the free flow of air in the airway. These moments of suffocation can last for up to an entire minute. A person with OSA wakes up hundreds of times during a single night as their bodies try not to suffocate. Snoring is their bodys only way to get enough air to breathe. ...
Some states, particularly members of the Non-Aligned Movement (NAM), see Article III on nonproliferation and Article X on promotion of peaceful use of biotechnology as equally important. They interpret Article X as an obligation for developed countries to share their expert knowledge with less-developed countries. Several states feel that developed countries have not fulfilled this obligation, and have called for the establishment of a formal mechanism for scientific cooperation. A few states also oppose the view that compliance with the BTWCs Article III necessitates national strategic trade controls, which in their view restrict the free flow of biological technology and knowledge.. Other states view disarmament and nonproliferation as the primary goal of the BTWC. Therefore, the aim of Article X is principally to ensure that the nonproliferation goals of the treaty do not hinder peaceful activities. These states are generally supportive of strategic trade controls and view them as necessary ...
If we are truly engaged in trying to understand the world we live in we need to busy ourselves with observation. Not looking properly, not seeing things are the common reasons why we fail to understand. We tend to be very quick at drawing conclusions without allowing the full experience of watching and observing to collect its information inside our system. And so it is with ear candling too. I have heard many explanations but sadly most of them dont even pass a simple observation test. However, when we observe we also have to guard ourselves, as much as we can, against wanting to see a certain thing and in doing so failing to see what is really important.. Lets put this into some practice. Take any type of ear candle you like and remove whatever device acts as a filter. This may be the little cross we find in the Biosun candles or it may be a tapered end, anything that partially obstructs the free flow of the air inside the candle. When cutting the candles above any sort of filter we have to ...
By Tony Perry, Los Angeles Times, January 22, 2008. The meeting between the Marines and the power brokers of this border region began with pleasantries, an exchange of gifts, and the drinking of small cups of tea, very hot and very sweet.. But within a few minutes the subject turned to one of crucial importance to both sides: the possible rise of militias among Sunni tribes who feel disrespected and shut out of the mild economic upturn the region is enjoying.. The power brokers - the mayor, the sheiks, and the local Iraqi army general - are from the Albu Mahal tribe, the most powerful in the region.. The Mahals were the first of the tribes to join with the U.S. in fighting the insurgency while lesser tribes stayed neutral or assisted the insurgents.. Now that the insurgency has been largely suppressed, Mahal leaders feel it is their right to share in the benefits of peace, such as the flourishing downtown market in Husaybah and the recently opened port of entry that allows a free flow of goods ...
This work is based on the theory that the Craniosacral and Fascial systems are not separate but are one interconnected system.. This blends craniosacral Therapy and Myofascial Release into a single and highly effective therapy.. A healthy system allows a healthy free flow of Cerebrospinal fluid from the brain , down the spine, to the sacrum, and into the connective tissue (fascia). Our brains need to expand and contract, and this is often blocked by stress, difficult birth or ...
Shared these thoughts and a few more with a reporter from CNN a few moments ago: The arrest of Henry Louis Gates sends a chilling message to the scholars, writers, activists, and artists who work so hard to keep a free flow of information. It seems eerily ironic Mr. Gates was returning from China, where surveillance is so high and freedom of speech and ideas so curtailed. To see the mugshot of Skip was a blow to all of us who feel some sense of safety based on our work to try to mend all of these broken fences in America--to make ourselves into people who refuse to be limited by race and class and gender and everything else. We do this work every day, and it is work, just like any other. To end up, at the end of the day, treated like a criminal, unjustly stripped of our accomplishments and contributions even if only for a moment, is profoundly disturbing. We must ask ourselves what it means, and to allow ourselves to face various scenarios regarding power and freedom and how these will ...
A common issue with tube feeding is snagging: door knobs, chairs, and otuer humans have been my top three culprits for sometimes a rather rough tug. And no. It does not feel very good.. There are a lot of people who use the button style tubes as either their G and/or J tubes because of the tube being low-profle, but in my experience, the button style tubes are superior in design and function. Even when accessed, these tubes arent as bulky and easier to conceal (which is good for those of us on continuous feeds). The extension tubing used to access the tubes are disposable, which is beneficial in respects to keeping things as sanitary as possible and in best functioning order. Extension tubing also has a clamp so there is only free flow of content when one is too absent minded to forget to squeeze the clamp shut and pump alarms are also as easy to fix as releasing the clamp (those are moments when you shake your head and say out loud to yourself Really?).. Here is another benefit of the ...
|p|The inhalation membrane wont cause a free flow in a counter current or when riding a scooter, which is a huge advantage of having the purge button on the side. It also gives the diver a freedom to select if the hose comes from the left or right withou
High-speed Internet is an absolute necessity, and the free flow of information is vital to our everyday lives. It impacts the way we live, work, study, and enjoy our free time. But right now, net neutrality rules that help ensure we have a free and open Internet are under attack. The FCC voted last year to repeal the rules that say Internet service... Read More ...
Plans to set up a transparent and easily accessible database with information on trade and markets in countries of East and Southern Africa could boost the free flow of goods and services in fields such as agriculture and health, experts have said.
I could not be mad or frustrated with him. He was just doing his job and the customer is not always right. Still, I wondered why this process was necessary and tried to process why there was a $10 service fee for cancelling the flight. The reality was, I needed to get home.. A one-way ticket when you have intentions of returning home can be a challenge, although the free flow of having no set plans has its perks of adrenaline. The other week, I was on a budget search for a plane ticket from Buenos Aires, Argentina, to Toronto, Canada. I could philosophize about whether my return was meant to be, but I knew I had to make it home as I had made a promise to a friend to return for her wedding and funds were running out.. So, I started looking for flights and a couple of weeks ago, I found one. I booked it. I wiped my hands of any stress. Until, that is, the e-mail came a week ago saying that it had been cancelled. Trying to get home became an enduring task mixed in with a little stress.. One night ...
Martuza said, The government is working tirelessly to curb corruption. The joint activities of the Information Commission and TIB will play a vital role in ensuring free flow of information and peoples right to information. TIB Executive Director said Prime Minister Sheikh Hasina has already identified the implementation of the right to information as a milestone. Information Commission and TIB will make the implementation of this act more effective ...
Instead of encouraging the free flow of data, research and results, the FDA is trying to muzzle it - especially when the exchange involves drugmakers truthfully discussing off-label uses.
Zyprexa adhd medication - they give a glimpse into the untold stories of the current war on cannabis, hemp and the free flow o...
Units may divide and recombine. This is done using the Divide / Recombine button in the Unit Report window (see 16.1), or from the popup menu you get by right clicking on the unit icon in the Map Panel. When you choose to divide a unit, it can be divided into either two or three sub-units at your discretion. In order to recombine into the original unit, all sub-units must be present. If any sub-unit is lost, the unit may not recombine until the sub-unit reappears as a reinforcement ...
Archives of Clinical Nephrology is an international, open access, peer-reviewed Journal, dedicated to the clinical and investigative studies, treatments, new diagnostic techniques, and other topics relating to the prevention, diagnosis, and treatment of disorders of the Kidney and its sub-units. The journal publishes novel updated research information dealing with the branch of medicine which deals with disorders related to Kidney and its sub-units.
By way of example, females endure a higher extent of endothelial and smooth muscle dysfunc tion in contrast with men. Hence, it has been suggested the endothel
This lab test measures the types of protein in the fluid (serum) part of a blood sample. Alternative Names: Lipoprotein electrophoresis Learn more here.
Electrophoresis Package 3/4 provides sufficient agarose, gel stains and electrophoresis buffers for up to 6 individual experiments. The package also includes the dye for rapid DNA staining during electrophoresis.. Contents of EP 3/4. ...
Alfa Aesar™ 1,4-Diacryloylpiperazine, Electrophoresis Reagent, 97% 1g Alfa Aesar™ 1,4-Diacryloylpiperazine, Electrophoresis Reagent, 97% Decylo...
Alfa Aesar™ BICINE, Electrophoresis Grade, ≥99% 100g Alfa Aesar™ BICINE, Electrophoresis Grade, ≥99% Goods Buffers
The basic principle of western blot are protein electrophoresis and ELISA. Electrophoresis is a commonly used method for separating proteins on the basis ..
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Electrophoresis. 13 (12): 960-9. doi:10.1002/elps.11501301199. PMID 1286667. S2CID 41855774. Ghetti A, Piñol-Roma S, Michael WM ...
Electrophoresis. 16 (7): 1160-9. doi:10.1002/elps.11501601192. PMID 7498159. S2CID 32209361. Nielsen SU, Spener F (Aug 1993). " ...
Electrophoresis. 13 (12): 960-9. doi:10.1002/elps.11501301199. PMID 1286667. S2CID 41855774. Kristensen P, Johnsen AH, Uerkvitz ...
Electrophoresis. 13 (12): 960-9. doi:10.1002/elps.11501301199. PMID 1286667. S2CID 41855774. Hattori H, Liu YC, Tohnai I, Ueda ...
Bamman, MM; Clarke, MS; Talmadge, RJ; Feeback, DL (March 1999). "Enhanced protein electrophoresis technique for separating ... human skeletal muscle myosin heavy chain isoforms". Electrophoresis. 20 (3): 466-8. doi:10.1002/(SICI)1522-2683(19990301)20:3. ...
Meyer, T. S.; Lambert, B. L. (1965). "Use of Coomassie brilliant blue R250 for the electrophoresis of microgram quantities of ... Altschul, A. M.; Evans, W. J. (1967). Zone electrophoresis with polyacrylamide gel. Methods in Enzymology. 11. pp. 179-186. doi ... This property can be used to separate proteins or protein complexes using polyacrylamide gel electrophoresis under non- ... Schägger, H.; Jagow, G. (1991). "Blue native electrophoresis for isolation of membrane protein complexes in enzymatically ...
Electrophoresis. 20 (8): 1786-1789. doi:10.1002/(sici)1522-2683(19990101)20:8. 3.0.co;2-5. PMID 10435450. "Brief Profiles of ... Electrophoresis. 20 (8): 1786-1789. doi:10.1002/(sici)1522-2683(19990101)20:8. 3.0.co;2-5. PMID 10435450. "Official Curriculum ...
Electrophoresis. 26 (1): 91-98. doi:10.1002/elps.200406129. PMID 15624129. Fuku, Noriyuki; Soo Park, Kyong; Yamada, Yoshiji; et ...
Electrophoresis. 15: 529-539. doi:10.1002/elps.1150150171. PMID 8055880. Brendel, V.; Bucher, P.; Nourbakhsh, I.R.; Blaisdell, ...
Electrophoresis. 20 (2): 241-8. doi:10.1002/(SICI)1522-2683(19990201)20:2. 3.0.CO;2-A. PMID 10197429. "Entrez Gene: BLCAP ...
Electrophoresis. 14 (10): 1023-31. doi:10.1002/elps.11501401163. PMID 8125050. S2CID 38041111. Berglund L, Björling E, Oksvold ...
Electrophoresis. 22 (10): 2103-9. doi:10.1002/1522-2683(200106)22:10. 3.0.co;2-w. PMID 11465512. Xi J, Wang X, Li S, Zhou X, ... gel electrophoresis with polyethylene glycol, affinity chromatography, and aggregation using DTT, though these methods are more ... "Polyethylene glycol fractionation improved detection of low-abundant proteins by two-dimensional electrophoresis analysis of ...
Electrophoresis. 34 (12): 1720-1728. doi:10.1002/elps.201300100. hdl:11343/44126. PMID 23592267. Conlan JV, Sripa B, Attwood S ...
Electrophoresis. 23 (14): 2247-2251. doi:10.1002/1522-2683(200207)23:14. 3.0.co;2-m. PMID 12210229. Fraser, M.P.; Yue, Z.W.; ...
... electrophoresis ... more than one hundred papers have been published during the past five years." Coulson's group consisted of ...
Electrophoresis. 26 (1): 91-98. doi:10.1002/elps.200406129. PMID 15624129. S2CID 44989190. Asari, M; et al. (2007). "Utility of ... Electrophoresis. 27 (22): 4408-18. doi:10.1002/elps.200600151. PMID 17058303. S2CID 28252456. Hongbin Yao, Mengge Wang, Xing ...
Shafer GE, Price MA, Tullius TD (1989). "Use of the hydroxyl radical and gel electrophoresis to study DNA structure". ... Electrophoresis. 10 (5-6): 397-404. doi:10.1002/elps.1150100518. PMID 2504579. S2CID 38355953. Galas DJ (November 2001). "The ...
Electrophoresis. 20 (4-5): 928-34. doi:10.1002/(SICI)1522-2683(19990101)20:4/5. 3.0.CO;2-Z. PMID 10344268. Etain B, Dumaine A, ...
"Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA". Electrophoresis. National Diagnostics. Retrieved 13 October 2016. ... These denaturants have been employed to make Denaturing Gradient Gel Electrophoresis gel (DGGE), which promotes denaturation of ...
"Comparison of RNA, single-stranded DNA and double-stranded DNA behavior during capillary electrophoresis in semidilute polymer ... solutions". Electrophoresis. nih.gov. 23 (7-8): 1033-44. doi:10.1002/1522-2683(200204)23:7/8. 3.0.CO;2-7. PMID 11981850. ...
In gel electrophoresis analysis, significant differences were found in the migration of cytoplasmic and mitochondrial HSP60. ... Electrophoresis. 11 (10): 883-91. doi:10.1002/elps.1150111019. PMID 2079031. S2CID 21541503. Jindal S, Dudani AK, Singh B, ... Electrophoresis. 15 (11): 1459-65. doi:10.1002/elps.11501501209. PMID 7895732. S2CID 33359306. Baca-Estrada ME, Gupta RS, Stead ... Electrophoresis. 13 (12): 992-1001. doi:10.1002/elps.11501301201. PMID 1286669. S2CID 23518983. Ikawa S, Weinberg RA (1992). " ...
Capillary electrophoresis coupled with ESI-MS is another technique; however, it works best when analyzing small amounts of ... Wang W, Sun J, Nimtz M, Deckwer WD, Zeng AP (2003). "Protein identification from two-dimensional gel electrophoresis analysis ... Whitmore, Colin D.; Gennaro, Lynn A. (2012-06-01). "Capillary electrophoresis-mass spectrometry methods for tryptic peptide ... the typical PMF samples are isolated proteins from two-dimensional gel electrophoresis (2D gels) or isolated SDS-PAGE bands. ...
Barnes et al., Electrophoresis in Practice: A Guide to theory and Practice. Page 44. VCH Publishers Inc, 1993 (USA) Görg, ... IPG increased reproducibility of Isoelectric focusing and 2D-Gel Electrophoresis. Other advantages are increased resolution, ... Görg, Angelika; Weiss, Walter; Dunn, Michael J. (2004-12-01). "Current two-dimensional electrophoresis technology for ... Electrophoresis. 15 (8-9): 1205-1211. doi:10.1002/elps.11501501182. ISSN 0173-0835. PMID 7532129. Blomberg, A.; Blomberg, L.; ...
Electrophoresis. 13 (12): 960-9. doi:10.1002/elps.11501301199. PMID 1286667. S2CID 41855774. Prasad GL, Valverius EM, McDuffie ...
Electrophoresis. 14 (11): 1216-22. doi:10.1002/elps.11501401181. PMID 8313870. S2CID 33424554. Ji H, Reid GE, Moritz RL, Eddes ... Electrophoresis. 13 (12): 992-1001. doi:10.1002/elps.11501301201. PMID 1286669. S2CID 23518983. Kaul SC, Wadhwa R, Matsuda Y, ... JS, Burgess AW, Simpson RJ (1997). "A two-dimensional gel database of human colon carcinoma proteins". Electrophoresis. 18 (3-4 ...
Electrophoresis. 13 (12): 960-9. doi:10.1002/elps.11501301199. PMID 1286667. Peddada LB, McPherson JD, Law R, Wasmuth JJ, ...
Electrophoresis. 21 (17): 3785-96. doi:10.1002/1522-2683(200011)21:17. 3.0.CO;2-2. PMID 11271497. Yin ZL, Dahlstrom JE, Le ...
"Identification of minor proteins of human colostrum and mature milk by two-dimensional electrophoresis". Electrophoresis. 19 ( ...
Electrophoresis. 18 (9): 1693-1700. doi:10.1002/elps.1150180938. PMID 9378147. Archived from the original (PDF) on 23 July 2013 ...
A microchip electrophoresis immunoaffinity assay for determination of serum thymidine kinase concentration has been described. ... Pagaduan JV, Ramsden M, O'Neill K, Woolley AT (2015). "Microchip immunoaffinity electrophoresis of antibody-thymidine kinase 1 ... complex". Electrophoresis. 36 (5): 813-7. doi:10.1002/elps.201400436. PMC 4346389. PMID 25486911. Doi S, Naito K, Yamada K ( ...
Capillary electrophoresis[edit]. In capillary electrophoresis, there is no solid matrix. Proteins are separated primarily by ... Serum protein electrophoresis (SPEP or SPE) is a laboratory test that examines specific proteins in the blood called globulins. ... Acetate or gel electrophoresis[edit]. Proteins are separated by both electrical forces and electroendoosmostic forces. The net ... Electrophoresis is a laboratory technique in which the blood serum (the fluid portion of the blood after the blood has clotted ...
As it reaches the anodic end of the electrophoresis medium electrophoresis is stopped. It can weakly bind to some proteins and ... Davis BJ, Ornstein L (1959). "A new high resolution electrophoresis method". Delivered at the Society for the Study of Blood at ... Electrophoresis[edit]. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental ... Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular ...
PRNewswire/ -- Reportlinker.com announces that a new market research report is available in its catalogue: Electrophoresis ... APPLICATIONS OF ELECTROPHORESIS 17. NUCLEIC ACID ELECTROPHORESIS 17. PROTEIN ELECTROPHORESIS 17. CHAPTER 4 TECHNOLOGY 20. BRIEF ... CHAPTER 5 MARKETS BY ELECTROPHORESIS TECHNOLOGY 27. CLASSIFICATION OF ELECTROPHORESIS TECHNOLOGIES 27. GEL ELECTROPHORESIS 27. ... CAPILLARY ELECTROPHORESIS 30. Capillary Zone Electrophoresis (CZE) 30. Capillary Gel Electrophoresis (CGE) 30. Micellar ...
Types of electrophoresis. There are quite a number of types of electrophoresis commonly used. It is not possible to go through ... The theory of electrophoresis. The concept here is simple enough. Like centrifugation, the molecules feel a force pushing them ... Electrophoresis. One common way of separating biological macro molecules is by taking advantage of the fact that many of these ... Another variation of gel electrophoresis is to pour a gel that purposely has a pH gradient from one end to the other. As the ...
Electrophoresis, the movement of electrically charged particles in a fluid under the influence of an electric field. If the ... Electrophoresis, also called cataphoresis, the movement of electrically charged particles in a fluid under the influence of an ... Electrophoresis is used to analyze and separate colloids (e.g., proteins) or to deposit coatings, as on elements used in ... protein: Electrophoresis. The positively and negatively charged side chains of proteins cause them to behave like amino acids ...
A hemoglobin electrophoresis can help diagnose diseases involving abnormal hemoglobin production, and often is performed as ... Are There Any Risks From Hemoglobin Electrophoresis Tests?. A hemoglobin electrophoresis test is a safe procedure with minimal ... When Are Hemoglobin Electrophoresis Test Results Ready?. Blood samples are processed by a machine, and it may take a day or two ... How Is a Hemoglobin Electrophoresis Test Done?. Most blood tests take a small amount of blood from a vein. To do that, a health ...
A hemoglobin electrophoresis can help diagnose diseases involving abnormal hemoglobin production, and often is performed as ... Blood Test: Hemoglobin Electrophoresis. Resources. Please Note: By clicking a link to any resource listed on this page, you ...
Hemoglobin electrophoresis measures the levels of the different types of this protein in the blood. ... Hb electrophoresis; Hgb electrophoresis; Electrophoresis - hemoglobin; Thallasemia - electrophoresis; Sickle cell - ... Hemoglobin electrophoresis measures the levels of the different types of this protein in the blood. ...
Hemoglobin electrophoresis is a test that measures different forms of hemoglobin in the blood. Its used to diagnose anemia, ... What is hemoglobin electrophoresis?. Hemoglobin is a protein in your red blood cells that carries oxygen from your lungs to the ... Hemoglobin electrophoresis is a test that measures the different types of hemoglobin in the blood. It also looks for abnormal ... Hemoglobin electrophoresis test results are often compared with other tests, including a complete blood count and a blood smear ...
Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have ... In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis ( ... Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry Kevin P. Bateman, John F. Kelly, Pierre ... Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry Pierre Thibault, Adèle Martin, ...
... particularly capillary electrophoresis efficiently in high sensitivity and in a short period of time. It also relates to a ... The invention relates to a method for separating a target for measurement utilizing electrophoresis, ... SDS-polyacrylamide electrophoresis, agarose gel electrophoresis, acrylamide electrophoresis, etc., or dielectrophoresis may ... SDS-polyacrylamide electrophoresis, agarose gel electrophoresis, acrylamide electrophoresis), dielectrophoresis, etc., column ...
Biochemie Biochemistry Clinical Biochemistry Electrophoresis Elektrophorese Enzyme Klinische Biochemie chemistry enzymes ...
The Spectrum Compact CE System is a personal capillary electrophoresis (CE) instrument that enables life scientists in ...
The aim of the Natural Sciences Bibliographic Web Portal including its new Dehydrogenase and Electrophoresis ... firstly the nucleic acid electrophoresis and secondly the electrophoresis of proteins. While nucleic acid electrophoresis is an ... Dehydrogenase & Electrophoresis Bibliography. Past News Releases. RSS. *EurekaMag.com Publishes 64,900 New... ... The aim of the Natural Sciences Bibliographic Web Portal including its new Dehydrogenase and Electrophoresis Bibliography is to ...
... Ayari Sahbi ayaris at MATHCN.UMontreal.CA Mon Jul 22 12:00:01 EST 1996 *Previous message: Where is the ...
... Barboni, Paola pbarboni at rvc.ac.uk Wed Nov 29 05:38:58 EST 2000 *Previous message: 4th ... Hello, I am looking for informations on capillary electrophoresis of proteins. I have just heard about this technique but I ...
We offer a wide choice of precast gels and buffers for protein electrophoresis (SDS PAGE). ... Protein electrophoresis is one of Lonzas key applications. ... Agarose for Protein Electrophoresis * PAGEr™ EX Gels for ... Lonza is an expert in protein electrophoresis as well as DNA and RNA electrophoresis, bringing a strong history of innovation ... Protein electrophoresis is a key part of your discovery process, and Lonza has the precast gels, stains and buffers you need. ...
The capillary electrophoresis device comprises a device body structure having a plurality of reservoirs arrayed thereon for ... The capillary electrophoresis chip comprises a straight main separation channel, an injection channel, and a plurality of ... A capillary electrophoresis device as well as a process for fabrication of the device is disclosed. ... of rows of grooves transversely defined to be connected with the reservoirs for receiving at least a capillary electrophoresis ...
A hemoglobin electrophoresis can help diagnose diseases involving abnormal hemoglobin production, and often is performed as ... A hemoglobin electrophoresis (eh-lek-truh-fer-EE-sis) blood test measures the different types of hemoglobin. Normal hemoglobin ... Why Are Hemoglobin Electrophoresis Tests Done?. Doctors may order the test to help diagnose conditions related to abnormal ... What Is a Hemoglobin Electrophoresis Test?. Hemoglobin (HEE-muh-glow-bin) is the protein inside red blood cells that carries ...
Purchase High-Resolution Electrophoresis and Immunofixation - 2nd Edition. Print Book & E-Book. ISBN 9780750694698, ... High-Resolution Electrophoresis Versus Standard Electrophoresis. Cost and High-Resolution Electrophoresis. Capillary ... Principles of High-Resolution Electrophoresis. High-Resolution Electrophoresis on Agarose. High-Resolution Electrophoresis on ... Two-Dimensional Electrophoresis. References. 3. Proteins in Serum Identified by High-Resolution Electrophoresis. Proteins ...
To inject sample into an electrophoresis capillary tube having a separating medium, the end of the capillary tube is inserted ... To permit electrophoresis, the electrophoresis section includes the capillary tube 30, the outlet moving mechanism 86, a second ... This invention relates to capillary electrophoresis. One class of capillary electrophoresis system includes as its principal ... the fan 19 and the electrophoresis section 22 mounted together for electrophoresis to illustrate the manner in which it may be ...
Gel electrophoresis is usually performed in labs to analyze DNA, RNA, or protein samples from various sources. ... Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such as DNA, RNA, and ... Applications of gel electrophoresis. Gel electrophoresis is widely used in the molecular biology and biochemistry labs in areas ... Principles of gel electrophoresis. The gel electrophoresis technique exploits the difference in size and charge of different ...
Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA fingerprint for a ... PFGE is different from conventional DNA electrophoresis because PFGE can separate very large fragments to generate a ... generic subtyping method for many different bacteria with only the choice of the restriction enzyme and electrophoresis ...
Thank you for your interest in spreading the word about Science.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
... In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St Louis ... Lipoprotein electrophoresis determines the amount of proteins made up of protein and fat, called lipoproteins (such as LDL ... Alternative Names: Lipoprotein electrophoresis Learn more here. ...
The Applied Biosystems SeqStudio Genetic Analyzer is a 4-capillary, fluorescence-based capillary electrophoresis system ... The Applied Biosystems SeqStudio Genetic Analyzer is a 4-capillary, fluorescence-based capillary electrophoresis system ...
multiplexed; capillary; electrophoresis; provides; side-entry; optical; excitation; geometry; charge-injection; device; ... Capillaries for use in a multiplexed capillary electrophoresis system Patent Yeung, Edward S. ; Chang, Huan-Tsang ; Fung, Eliza ... The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A ... The invention provides a side-entry optical excitation geometry for use in a multiplexed capillary electrophoresis system. A ...
The SEA 2000 Electrophoresis Apparatus (patented) provides unparalleled reproducibility, speed and convenience for your DNA and ... RNA electrophoresis.,,,,,, In combination with Elchrom Scientific AG ready-to-use gels the SEA 2000 covers applications that ... Elchrom Submerged Gel Electrophoresis Advanced System. 8. Shorter Mini Horizontal Gel Electrophoresis System. 9. Mini EZRun ... Pore Gradient Lipoprotein Electrophoresis System (PGGE). 4. PSE Electrophoresis Power Supply. 5. Elchrom Submerged Gel ...
Browse our large selection of Gel Electrophoresis Reagents & Biotechnology at Nasco. You will find a unique blend of products ... All Products / Nasco / Education Supplies / Science / Biotechnology / Gel Electrophoresis Reagents Displaying items: 1 - 3 of 3 ...
Results for electrophoresis equipment from Biometra, CreGen, Cytiva and other leading brands. Compare and contact a supplier ... MicruX iHVStat - Electrophoresis Systems. iHVStat brings the new evolution of electrophoresis systems based on microfluidic ... Biometra - Model Horizon Family - Agarose Gel Electrophoresis Systems. either directly in the electrophoresis device or in an ... Capel - Model 105M - Capillary Electrophoresis System. Capel-105M is a capillary electrophoresis system for fast and effective ...
  • This report also assesses the growth and market potential for agarose and polyacrylamide gel electrophoresis techniques. (prnewswire.com)
  • The gel used in gel electrophoresis is usually made of a material called agarose, which is a gelatinous substance extracted from seaweed. (news-medical.net)
  • The Biometra Rotaphor is a rotating field electrophoresis systemspecifically designed for the high resolution separation of large DNA molecules up to 8 MB in agarose gels under controlled temperatur conditions. (environmental-expert.com)
  • Electrophoresis performed in a gel composed of agarose, polyacrylamide, or starch. (thefreedictionary.com)
  • In addition to size separation, nucleic acid fractionation using agarose gel electrophoresis can be an initial step for further purification of a band of interest. (ipl.org)
  • Agarose for nucleic acid electrophoresis, immunodiffusion, and double-diffusion Ouchterlony plates. (mpbio.com)
  • Developing the experimental technologies used to study DNA fragments: agarose gel electrophoresis and restriction enzymes. (dnalc.org)
  • antibodies-online is proud to announce that we now offer high-quality, ready-to-use, affordable molecular weight markers for both agarose and acrylamide gel electrophoresis experiments. (antibodies-online.com)
  • Designed for fast and convenient E-Gel™ agarose gel electrophoresis, from DNA sample loading to gel image. (fishersci.ca)
  • Select Thermo Scientific™ Owl™ power supplies for horizontal (agarose) or vertical (polyacrylamide) gel electrophoresis systems of various kinds, whether tanks, chambers, sequencers, or blotters. (thermofisher.com)
  • Serum protein electrophoresis ( SPEP or SPE ) is a laboratory test that examines specific proteins in the blood called globulins . (wikipedia.org)
  • [1] The most common indications for a serum protein electrophoresis test are to diagnose or monitor multiple myeloma , a monoclonal gammopathy of uncertain significance (MGUS), or further investigate a discrepancy between a low albumin and a relatively high total protein. (wikipedia.org)
  • Electrophoresis is a laboratory technique in which the blood serum (the fluid portion of the blood after the blood has clotted) is applied to an acetate membrane soaked in a liquid buffer. (wikipedia.org)
  • Normal serum protein electrophoresis diagram with legend of different zones. (wikipedia.org)
  • High-Resolution Electrophoresis and Immunofixation: Techniques and Interpretation, Second Edition presents the important developments in the technology of serum protein electrophoresis. (elsevier.com)
  • For development of any marker proteins from complex biological fluids, such as plasma, serum and urine, two-dimensional (2D) gel electrophoresis technique is routinely used in many laboratories (Bichsel et al. (thefreedictionary.com)
  • This phenomenon was first observed by Ferdinand Frederic Reuss followed by Arne Tiselius who won the Nobel Prize in chemistry for his research on electrophoresis, adsorption analysis and his discoveries concerning the complex nature of serum protein . (ipl.org)
  • Effective August 30, 2016, Legacy Laboratory Services updated reference ranges for serum protein electrophoresis due to an instrumentation change. (legacyhealth.org)
  • Serum is the preferred sample for electrophoresis. (cornell.edu)
  • However, guidance is lacking on clinical reporting of serum (SPE), urine (UPE) protein electrophoresis and immunotyping. (aacc.org)
  • The serum globulin electrophoresis test measures the levels of proteins called globulins in the fluid part of a blood sample. (floridahealthfinder.gov)
  • Capillary electrophoresis, rapid and inexpensive, seems a promising alternative to high-performance liquid chromatography (HPLC) for creatinine determination in human serum. (nih.gov)
  • Serum protein electrophoresis is often normal or may show a broad monoclonal band. (medscape.com)
  • Serum protein electrophoresis (SPEP) or urine protein electrophoresis (UPEP) and immunofixation are essential tests. (medscape.com)
  • In these cases, isolating the monoclonal proteins from the serum, treating them with reducing agents to cleave disulfide bonds, and subjecting them to gel electrophoresis to determine the size of the immunoglobulin heavy chain polypeptide may be necessary to confirm mu-HCD. (medscape.com)
  • Serum protein electrophoresis is used to identify patients with multiple myeloma and other serum protein disorders. (aafp.org)
  • Many subspecialists include serum protein electrophoresis screening in the initial evaluation for numerous clinical conditions. (aafp.org)
  • This article provides a comprehensive review of serum protein electrophoresis, including a discussion of how the examination is performed, what it measures, and when it is indicated. (aafp.org)
  • Several subsets of serum protein electrophoresis are available. (aafp.org)
  • The pattern of serum protein electrophoresis results depends on the fractions of two major types of protein: albumin and globulins. (aafp.org)
  • The subsets of these proteins and their relative quantity are the primary focus of the interpretation of serum protein electrophoresis. (aafp.org)
  • Figure 1 shows a typical normal pattern for the distribution of proteins as determined by serum protein electrophoresis. (aafp.org)
  • Typical normal pattern for serum protein electrophoresis. (aafp.org)
  • Despite the advent of several alternative analytical technologies for separation, electrophoresis is likely to continue to serve as a complementary technology for separating biologically important macromolecules. (prnewswire.com)
  • The role of electrophoresis as an analytical separation method for both nucleic acid and protein molecules was examined. (prnewswire.com)
  • BCC analyzes the impact of the two major electrophoresis techniques on biological molecular separation, determines their current market status, examines their future potential and presents forecasts of growth over the next five years. (prnewswire.com)
  • Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. (springer.com)
  • Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. (springer.com)
  • Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis. (springer.com)
  • Lonza also offers traditional protein electrophoresis products like Laemmli Tris-Glycine gels for polyacrylamide gel electrophoresis (PAGE) along with high quality prepared Tris-Glycine buffer for separation of native proteins and Tris-Glycine-SDS buffer for denatured proteins (SDS PAGE). (lonza.com)
  • The capillary electrophoresis chip comprises a straight main separation channel, an injection channel, and a plurality of sample transport channels defined thereon in liquid communication with the reservoirs. (google.com)
  • Forced flow electrophoresis allows the separation, in an aqueous medium, of compounds which are mobile in an electric field. (google.com)
  • The Qsepl 00 DNA Analyzer from Precision Biosystems uses micro-capillary gel electrophoresis combined with fluorescence detection to perform post-PCR separation, detect DNA and RNA fragments and assess the quality of genomic DNA. (thefreedictionary.com)
  • A form of electrophoresis in which a gel or membrane is run in one direction, rotated 90 o , and run again under a different set of conditions (so as to gain a different separation of components). (everything2.com)
  • 2D gel electrophoresis is a powerful technique for protein separation, purification, and analysis. (everything2.com)
  • Hemoglobin electrophoresis has been superseded by more rapid, sensitive, and quantitative methods of hemoglobin separation. (medscape.com)
  • Embodiments of a device and method are described which provide for control over the distortion of a sample zone upon exiting an electrophoresis separation channel. (freepatentsonline.com)
  • Traditional sample injection in capillary electrophoresis involves physical manipulation of the separation capillary into and out of vials containing buffer or sample, making the process slow and difficult to reproduce. (photonics.com)
  • Gel electrophoresis is a technique that allows separation of DNA, RNA and protein segments based on size. (flinnsci.com)
  • Separation by electrophoresis depends on differences in the migration velocity of ions or solutes through a given medium in an electric field. (wikibooks.org)
  • Researchers at Kent State University have devised an electrophoresis technique using liquid crystals that could prove useful as a separation method for biological material like proteins. (genomeweb.com)
  • Electrophoresis may be the main technique for molecular separation in today's cell biology laboratory. (gac.edu)
  • Electrophoresis can be one dimensional (i.e. one plane of separation) or two dimensional. (gac.edu)
  • There are a number of different types of electrophoresis, but all involve generating an electric field between to points and placing a matrix of some sort in-between through which the macromolecules must travel. (asu.edu)
  • Types of electrophoresis. (asu.edu)
  • There are quite a number of types of electrophoresis commonly used. (asu.edu)
  • In the field of biochemistry, there are two types of electrophoresis, firstly the nucleic acid electrophoresis and secondly the electrophoresis of proteins. (prweb.com)
  • There are many different types of electrophoresis, and each can be used for something different. (wikibooks.org)
  • For specific types of electrophoresis (for example, the process of administering medicine, iontophoresis ), see electrophoresis (disambiguation) . (wikidoc.org)
  • The SEA 2000 Electrophoresis Apparatus (patented) provides unparalleled reproducibility, speed and convenience for your DNA and RNA electrophoresis. (bio-medicine.org)
  • The present invention relates to a method and to an apparatus for forced flow electrophoresis. (google.com)
  • The SEA 2000 apparatus and ready-to-use gels are the key components of Elchrom's system for analysis of nucleic acids by submerged gel electrophoresis. (bio-medicine.org)
  • Therefore, this brief review will focus on the use of gel electrophoresis linked to mass spectrometry and bioassays as a useful approach for detecting and characterizing LPS aggregates. (thefreedictionary.com)
  • Capillary electrophoresis (CE)-mass spectrometry (MS) is one such technology that integrates sensitivity, label-free detection, and capability for quantification. (rsc.org)
  • Polyacrylamide gel electrophoresis ( PAGE ) is a technique widely used in biochemistry , forensic chemistry , genetics , molecular biology and biotechnology to separate biological macromolecules , usually proteins or nucleic acids , according to their electrophoretic mobility . (wikipedia.org)
  • This book discusses the electrophoretic patterns that one encounters when using high-resolution electrophoresis and immunofixation. (elsevier.com)
  • Affinity electrophoresis Electrophoretic deposition Capillary electrophoresis Dielectrophoresis Electroblotting Gel electrophoresis Gel electrophoresis of nucleic acids Immunoelectrophoresis Isoelectric focusing Isotachophoresis Pulsed-field gel electrophoresis Nonlinear frictiophoresis Lyklema, J. (1995). (wikipedia.org)
  • Retain settings after shut-down with the power-off memory on the Thermo Scientific™ Owl™ EC300XL2 Compact Power Supply, ideal for DNA/RNA and Protein electrophoresis. (fishersci.ca)
  • Eliminate comb assembly with heavy-duty, one-piece Thermo Scientific™ Owl™ EasyCast™ B1A Mini Gel and A2-OK Multi Gel Electrophoresis System Combs. (fishersci.ca)
  • Eliminate comb assembly with this assortment of heavy-duty one-piece combs, for use with the Thermo Scientific™ Owl™ EasyCast™ B1 Mini Gel Electrophoresis System. (fishersci.ca)
  • Eliminate comb assembly with Thermo Scientific™ Owl™ EasyCast™ B2 and B3 Mini Gel Electrophoresis System Combs. (fishersci.ca)
  • Perform homogeneous or gradient polyacrylamide gel electrophoresis of nucleic acids or proteins using rigs, casters, plates, spacers, combs, blotters, power supplies, and other accessories of Thermo Scientific™ Owl™ Vertical Gel Electrophoresis Systems. (thermofisher.com)
  • Thermo Scientific™ Owl™ S3S (wide) and S4S (narrow) sequencing gel rigs, SGC gel casting system, and their accessories enable polyacrylamide gel electrophoresis for the purpose of DNA sequencing. (thermofisher.com)
  • Thermo Scientific™ Owl™ P-Series gel systems, JGC casters and related accessories enable casting and running of single-percent and gradient polyacrylamide gels for nucleic acid or protein electrophoresis. (thermofisher.com)
  • Select Thermo Scientific™ Owl™ electrophoresis devices, accessories, and supplies for wet or semi-dry blotting, staining, or drying and storing of polyacrylamide gels. (thermofisher.com)
  • Thermo Scientific Owl products provide optimal laboratory throughput and efficiency for gel electrophoresis. (thermofisher.com)
  • A fluorescence detection system for capillary electrophoresis is provided wherein the detection system can simultaneously excite fluorescence and substantially simultaneously monitor separations in multiple capillaries. (osti.gov)
  • The P/ACE Series is an automated, programmable capillary electrophoresis system designed to perform fast separations from complex samples. (environmental-expert.com)
  • GEL ELECTROPHORESIS is the general and most used method, for the separations, identification and analysis of nucleic acids and proteins. (thefreedictionary.com)
  • The utility of these media is illustrated by separations of linear, double-stranded DNA up to 3,000 bp long by conventional electrophoresis, and of single-stranded DNAs from 4 to 60 nt long by capillary electrophoresis. (mendeley.com)
  • Extraordinary separations of supercoiled DNAs were also obtained by capillary electrophoresis. (mendeley.com)
  • One dimensional electrophoresis is used for most routine protein and nucleic acid separations. (gac.edu)
  • Recently, Lonza introduced our novel PAGEr™ EX ProSieve™ EX products for fast protein electrophoresis and staining or rapid tank transfer for western blotting. (lonza.com)
  • Ideal for PAGE electrophoresis and blotting applications. (environmental-expert.com)
  • With Biometra Eco-Line, Analytik Jena offers a robust, modular tank system for PAGE electrophoresis and blotting applications to support you in your protein analysis. (environmental-expert.com)
  • Tris-Glycine buffer for protein gel electrophoresis and western blotting. (mpbio.com)
  • Protein electrophoresis is a key part of your discovery process, and Lonza has the precast gels, stains and buffers you need. (lonza.com)
  • Specially designed for everyday use, gels can be casted quickly, easily and in a space-saving manner in the electrophoresis module. (environmental-expert.com)
  • Liquabis is mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis. (mpbio.com)
  • One, column electrophoresis, uses tubular gels formed in glass tubes, while the other, slab gel electrophoresis, uses flat gels formed between two plates of glass. (gac.edu)
  • Reiner Westermeier worked after PhD graduation and Post-doc at the Technische Universität München for 30 years as a specialist for electrophoresis methods for leading bioanalytics and biotechnology companies. (weltbild.de)
  • While nucleic acid electrophoresis is an analytical technique that allows a scientist or researcher to separate DNA or RNA fragments according to their size and reactivity, protein electrophoresis uses a fluid or an extract in order to analyze the proteins contained within. (prweb.com)
  • The Biometra Multigel product family includes 3 different double-gel models for gel-based high-resolution protein and nucleic acid electrophoresis . (environmental-expert.com)
  • What Is a Hemoglobin Electrophoresis Test? (kidshealth.org)
  • A hemoglobin electrophoresis test measures the different types of hemoglobin in the blood. (kidshealth.org)
  • How Should We Prepare for a Hemoglobin Electrophoresis Test? (kidshealth.org)
  • This can affect the hemoglobin electrophoresis test. (kidshealth.org)
  • Can I Stay With My Child During a Hemoglobin Electrophoresis Test? (kidshealth.org)
  • How Long Does a Hemoglobin Electrophoresis Test Take? (kidshealth.org)
  • When Are Hemoglobin Electrophoresis Test Results Ready? (kidshealth.org)
  • A hemoglobin electrophoresis test is a safe procedure with minimal risks. (kidshealth.org)
  • If you have questions about the hemoglobin electrophoresis test, speak with your doctor or the health professional doing the blood draw. (kidshealth.org)
  • A hemoglobin electrophoresis test applies an electric current to a blood sample. (medlineplus.gov)
  • You don't need any special preparations for a hemoglobin electrophoresis test. (medlineplus.gov)
  • If you have questions about the hemoglobin electrophoresis test or what the test results mean, talk to your doctor. (kidshealth.org)
  • The Applied Biosystems SeqStudio Genetic Analyzer is a 4-capillary, fluorescence-based capillary electrophoresis system designed to provide users with an integrated experience that is easy, efficient, and versatile for research in genetic analysis. (fishersci.com)
  • Your capillary electrophoresis (CE) genetic analyzer is more powerful than you may think. (thermofisher.com)
  • As with all forms of gel electrophoresis , molecules may be run in their native state , preserving the molecules' higher-order structure. (wikipedia.org)
  • Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the difference of their molecular weight. (wikipedia.org)
  • At the pH at which gel electrophoresis is carried out the SDS molecules are negatively charged and bind to proteins in a set ratio, approximately one molecule of SDS for every 2 amino acids. (wikipedia.org)
  • Scien.net has now newly included the category Dehydrogenase which which is an enzyme that catalyzes the removal and eventual transfer of hydrogen molecules from a substrate in an oxidation and reduction reaction, and on Electrophoresis, which is a process used in biochemistry to determine and evaluate proteins or enzymes within a specific sample. (prweb.com)
  • The gel electrophoresis technique exploits the difference in size and charge of different molecules in a sample. (news-medical.net)
  • Gel electrophoresis is a technique that involves the movement of molecules through a gelatin-like material in an electrical field. (flinnsci.com)
  • In gel electrophoresis, large molecules are going to move slower. (wikipedia.org)
  • Electrophoresis is a laboratory procedure that separates molecules. (livestrong.com)
  • If done correctly, electrophoresis will separate all molecules in a specimen. (livestrong.com)
  • It controls the pump of the SEA 2000 unit, delaying the start automatically for a few minutes after the beginning of electrophoresis. (bio-medicine.org)
  • Analysis of Sample Injection and Brand-Broadening in Capillary Electrophoresis Microchips," Techinal Proceedings of the MSM 2000, San Diego, CA, March 27-29, 2000. (freepatentsonline.com)
  • Difference gel electrophoresis (DIGE) is a gel electrophoresis-based technique for protein quantification in complex mixtures. (diva-portal.org)
  • One of the most common means of analyzing proteins by electrophoresis is by using Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis. (asu.edu)
  • USPRwire, Thu Aug 08 2019] Polyacrylamide Gel Electrophoresis (PAGE) is a typical technique for isolating proteins by electrophoresis. (thefreedictionary.com)
  • Our protein gel electrophoresis service offers you a range of techniques for various applications in biopharmaceutical product characterization, such as measuring molecular weight and characterizing impurities. (sgs.com)
  • As the world's leading provider of protein gel electrophoresis services, we provide the experience, technical and regulatory expertise, and unique global network you need. (sgs.com)
  • Protein gel electrophoresis is the commonly used technology to separate proteins according to their physical properties such as electrical charge and molecular weight etc. (genscript.com)
  • 15. The detection system of claim 1 wherein at least one of the capillaries contains a gel that is suitable for capillary gel electrophoresis systems. (google.ca)
  • End-Column Detection for Capillary Zone Electrophoresis," Anal. (freepatentsonline.com)
  • Axial-beam absorbance detection for capillary electrophoresis," Journal of Chromatography, 550(1991) pp. 831-837. (freepatentsonline.com)
  • Capillary Electrophoresis Chips with Integrated Electrochemical Detection," Anal. (freepatentsonline.com)
  • When added to buffer running through a capillary electrophoresis system, the analyte was effectively invisible to the filtered 488-nm detection beam from an argon-ion laser made by Coherent Inc. of Santa Clara, Calif. (photonics.com)
  • Capillary electrophoresis with a universal UV-based product detection is a promising analytical tool to quantify product formation. (nature.com)
  • Extending the previous preliminary study on the construction of a capillary electrophoresis (CE)/sensor for the detection of reducing analytes, we focus the interest on the simultaneous detection of redox active species, which are important indicators of the oxidative damage in tissues, of food preservation, and of pollution. (diva-portal.org)
  • His area of responsibility included co-operation in product development, writing of scientic papers and method instructions, trouble shooting in customer laboratories, performing seminars and practical courses on electrophoresis and proteomics, as well as giving scientific talks at congresses on a world-wide basis. (weltbild.de)
  • Other chapters consider some detailed studies of strategies employing immunoglobulin quantification and high-resolution electrophoresis to detect monoclonal gammopathies. (elsevier.com)
  • Laboratories worldwide use protein electrophoresis to diagnose monoclonal gammopathies. (aacc.org)
  • The Canadian Society of Clinical Chemists' (CSCC) Monoclonal Gammopathy Working Group (MGWG) decided to take action, forming a working group and developing a series of candidate recommendations to standardize protein electrophoresis reporting, which have published in Clinical Biochemistry . (aacc.org)
  • Maisnar et al presented a case study in which they used immunofixation electrophoresis, capillary zone electrophoresis with immunotyping, and high-resolution two-dimensional electrophoresis to detect and characterize monoclonal mu-heavy chains in a patient with multiple malignancies. (medscape.com)
  • Applied Biosystems genetic analysis systems are a trusted standard for Sanger sequencing and fragment analysis by capillary electrophoresis-proven through decades of results, including the first sequencing of the human genome and the discovery of genes implicated in diseases like cystic fibrosis. (thermofisher.com)
  • Applied Biosystems genetic analyzers for DNA sequencing and fragment analysis applications feature proven capillary electrophoresis technology and utilize our high-quality capillaries, polymers, and BigDye Terminator chemistry. (thermofisher.com)
  • A hemoglobin electrophoresis (eh-lek-truh-fer-EE-sis) blood test measures the different types of hemoglobin. (kidshealth.org)
  • Hemoglobin electrophoresis is a blood test that can detect different types of hemoglobin. (wikipedia.org)
  • Electrophoresis of positively charged particles (cations) is sometimes called cataphoresis, while electrophoresis of negatively charged particles (anions) is sometimes called anaphoresis. (wikipedia.org)
  • Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA fingerprint for a bacterial isolate. (cdc.gov)
  • She appreciated the efforts of HEC, Pakistan for generous support in the establishment of first ever genetics laboratory of Department of Zoology with latest equipment including PCR thermo cycler, Gel electrophoresis , PH meter, digital weight balance, Water bath, Vortex machine, centrifuge machines, Gel documentation system and others. (thefreedictionary.com)
  • Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. (routledge.com)
  • The improvements in this field enable technicians in a laboratory to perform electrophoresis will little effort. (livestrong.com)
  • Due to the ubiquitous nature of gel electrophoresis in experimental biology, nucleotide and protein molecular weight standards for electrophoresis are among the most important common-use laboratory products. (antibodies-online.com)
  • Evaluation of a suspected hemoglobinopathy should include electrophoresis of a hemolysate to detect abnormal hemoglobins and quantification of HbA 2 and HbF with column chromatography. (medscape.com)
  • Development of a fast capillary electrophoresis method for the determination of propranolol-total analysis time reduction strategies," Journal of Chromatography A , vol. 1216, no. 45, pp. 7957-7961, 2009. (hindawi.com)
  • The final chapter deals with the principles of high-resolution electrophoresis and immunofixation electrophoresis (IFE). (elsevier.com)
  • Exceed your expectations with an automated electrophoresis system from Agilent. (agilent.com)
  • Learn more about the nine automated electrophoresis instruments Agilent offers as well as the intuitive software and accessories that will take your research to new heights. (agilent.com)
  • Fluorophore assisted carbohydrate electrophoresis diagnosis may be applied to various patient specimens, including blood, urine and skin. (google.ca)
  • Although electrophoresis is a standardized technique, there have been considerable technology advances because of its ever-expanding research applications. (prnewswire.com)
  • About 1930 the Swedish chemist Arne Tiselius introduced the use of electrophoresis as an analytic technique. (britannica.com)
  • Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such as DNA, RNA, and proteins. (news-medical.net)
  • This technique is a type of electrophoresis used to separate hemoglobin proteins from each other with an electric current . (everything2.com)
  • Capillary electrophoresis (CE) is a powerful analytical technique that is widely used in research and development and in quality control of pharmaceuticals. (elsevier.com)
  • An analytical technique in which the motion of scattered particles run through a fluid under the influence of uniformly charged field is called electrophoresis. (ipl.org)
  • Since the distance that a molecule will move is dependent on its size, gel electrophoresis is a good technique to separate different size DNA fragments. (flinnsci.com)
  • Gel electrophoresis is a technique used to separate mixtures like DNA and proteins . (wikipedia.org)
  • Gel electrophoresis is the most commonly used technique to study DNA . (wikipedia.org)
  • DNA Gel Electrophoresis is a technique used to separate and identify DNA fragments based on size. (jove.com)
  • Electrophoresis is a fast and easy technique. (livestrong.com)
  • 2D Electrophoresis (2D-E) is an efficient and reliable technique that has been widely used to separate proteins by the bioscientists. (actapress.com)
  • Electrophoresis is a common molecular biology technique that is used to separate biological materials by virtue of their size and/or charge. (antibodies-online.com)
  • The protein electrophoresis blood test may be ordered as a follow up to abnormal findings on lab tests such as total protein, albumin, elevated calcium levels or low white and red blood cell counts. (healthtestingcenters.com)
  • Salivary protein profiles of 43 patients and 39 healthy control subjects were compared on three different gel electrophoresis systems: sodium dodecylsulfate-polyacrylamide gel electrophoresis, anionic polyacrylamide gel electrophoresis, and immobilized pH gradient gel electrophoresis (isoelectric point, 3.5-5.0). (nih.gov)
  • The Biometra Compact Multi-Wide is the latest member of the Compact Family and completes the electrophoresis portfolio for the identification, quantification and purification of nucleic acid fragments. (environmental-expert.com)
  • In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. (springer.com)
  • The Spectrum Compact CE System is a personal capillary electrophoresis (CE) instrument that enables life scientists in laboratories of all sizes to perform Sanger sequencing and fragment analysis at the bench. (genengnews.com)
  • It features posts on kinetic properties of NADP+-malic enzyme in two populations of the C4 weed species Echinochloa crusgalli (L.) (Poaceae) from sites of contrasting climates, isolation of the cellulase enzymes from the thermophilic fungus Thermoascus aurantiacus and regulation of enzyme production, on isoenzyme analysis of Leishmanian strains by electrophoresis on acetate-cellulose plates, and on enzyme variability in wild wheat populations. (prweb.com)
  • Electrophoresis is used extensively in DNA, RNA and protein analysis. (wikipedia.org)
  • Summary: Fact.MR has announced the addition of the " Pulsed Field Gel Electrophoresis (PFGE) Systems Market Forecast, Trend Analysis & Competition Tracking - Global Review 2018 to 2028"report to their offering. (thefreedictionary.com)
  • During your biopharmaceutical product characterization, our Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis provides you with a relatively inexpensive yet efficient method for visualizing protein mixtures. (sgs.com)
  • Why choose gel electrophoresis analysis from SGS? (sgs.com)
  • To find out more about our gel electrophoresis analysis, contact us today. (sgs.com)
  • DNA analysis is just one possible application for electrophoresis. (livestrong.com)
  • Microchip Electrophoresis System No gel creation required and automated analysis of up to 108 loaded samples. (tradeindia.com)
  • Researchmoz added Most up-to-date research on "Electrophoresis Equipment and Supplies Market: Global Industry Analysis 2013-2017 and Opportunity Assessment 2018-2028" to its huge collection of research reports. (openpr.com)
  • Hemoglobin electrophoresis measures hemoglobin levels and looks for abnormal types of hemoglobin. (medlineplus.gov)
  • Hemoglobin electrophoresis is used as a screening test to identify normal and abnormal hemoglobins and assess their quantity. (medscape.com)
  • The protein electrophoresis blood test identifies the presence of abnormal proteins, identify normal proteins, and to determine if groups of proteins are unusually high or low in the blood. (healthtestingcenters.com)
  • Electrophoresis has been the method of choice for several decades to physically separate macromolecules such as nucleic acid fragments, RNA and proteins according to their charge and molecular size. (prnewswire.com)
  • PFGE is different from conventional DNA electrophoresis because PFGE can separate very large fragments to generate a fingerprint by constantly changing the direction of the electric field. (cdc.gov)
  • In DNA fingerprinting, a gel electrophoresis can be done to separate the fragments. (ehow.co.uk)
  • One disadvantage in using a gel electrophoresis for DNA fingerprinting is that the fragments have to be made in a specific manner. (ehow.co.uk)
  • After the resulting DNA fragments are run through electrophoresis, the distance between bands are measured and recorded as the DNA "fingerprint. (wikidoc.org)
  • Anionic surfactants are often used to denature proteins before electrophoresis. (wikibooks.org)
  • Protein electrophoresis separates proteins based on their size and electrical charge which are then turned into patterns or bands. (healthtestingcenters.com)
  • Electrophoresis separates proteins based on their physical properties, and the subsets of these proteins are used in interpreting the results. (aafp.org)
  • Ethidium bromide is a fluorescent dye commonly used in gel electrophoresis. (news-medical.net)
  • Alkaline and/or citrate agar electrophoresis is the commonly used method. (medscape.com)
  • More commonly, the proteins are present in smaller amounts and show a heterogeneous pattern on electrophoresis. (medscape.com)
  • In most proteins, the binding of SDS to the polypeptide chains impart an even distribution of charge per unit mass, thereby resulting in a fractionation by approximate size during electrophoresis. (wikipedia.org)
  • A continuous forced flow electrophoresis cell and a method of operation for the fractionation of an aqueous liquid, such as blood, containing at least two compounds, the relative mobilities of which in an electric field vary as a function of the pH, in order to obtain one fraction enriched and one depleted. (google.com)
  • Electrophoresis is used in laboratories to separate macromolecules based on size. (wikipedia.org)
  • Polyacrylamide gel electrophoresis is a powerful tool used to analyze RNA samples. (wikipedia.org)
  • When polyacrylamide gel is denatured after electrophoresis, it provides information on the sample composition of the RNA species. (wikipedia.org)
  • Two-dimensional polyacrylamide gel electrophoresis. (wikibooks.org)
  • When separated on a polyacrylamide gel, the procedure is abbreviated as SDS--PAGE (for Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis). (gac.edu)
  • BCC was also interested in examining end-users' perspectives in various industrial applications that use electrophoresis. (prnewswire.com)
  • We have a variety of concentrations and well formats to meet the needs for various protein electrophoresis applications. (lonza.com)
  • Electrophoresis of proteins and nucleic acids: II--Techniques and applications. (bmj.com)
  • Hayes J. D. , Stockman P. K. . Electrophoresis of proteins and nucleic acids: II--Techniques and applications. (bmj.com)
  • Vertical Electrophoresis System - Mini It is required in biotechnology, Molcular Biology applications in colleges, Universities, Research Institutes. (tradeindia.com)
  • This book contains 20 papers describing techniques and applications of cell electrophoresis. (annals.org)
  • When doctors suspect a patient has a genetic blood disorder, such as sickle cell anemia, hemoglobin electrophoresis separates the hemoglobin types for examination. (livestrong.com)
  • Cellulose acetate (CA) electrophoresis at pH 8.2-8.6 can be used to resolve common variants such as HbS and HbC, but it cannot be used to distinguish between HbS and HbD Punjab and HbG Philadelphia . (medscape.com)
  • Electrophoresis is done by the use of cellulose acetate. (wikipedia.org)
  • After running electrophoresis at 150 to 200 volts, stain the cellulose acetate gel with Ponceau red. (wikipedia.org)
  • In zone electrophoresis, for example, different protein subtypes are placed in separate physical locations on a gel made from agar, cellulose, or other plant material. (aafp.org)