DNA, Ribosomal
DNA, Ribosomal Spacer
DNA, Intergenic
RNA, Ribosomal, 23S
RNA, Ribosomal, 5.8S
RNA, Ribosomal
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
Nebulizers and Vaporizers
Sequence Analysis, DNA
RNA, Ribosomal, 16S
Metered Dose Inhalers
Clustered Regularly Interspaced Short Palindromic Repeats
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Prosthesis-Related Infections
Polymorphism, Restriction Fragment Length
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Genes, rRNA
Albuterol
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
RNA, Ribosomal, 18S
Chlorofluorocarbons
Bone Cements
Adhesives used to fix prosthetic devices to bones and to cement bone to bone in difficult fractures. Synthetic resins are commonly used as cements. A mixture of monocalcium phosphate, monohydrate, alpha-tricalcium phosphate, and calcium carbonate with a sodium phosphate solution is also a useful bone paste.
Administration, Inhalation
Bronchodilator Agents
RNA, Ribosomal, 28S
Sequence Homology, Nucleic Acid
Inverted Repeat Sequences
Copies of nucleic acid sequence that are arranged in opposing orientation. They may lie adjacent to each other (tandem) or be separated by some sequence that is not part of the repeat (hyphenated). They may be true palindromic repeats, i.e. read the same backwards as forward, or complementary which reads as the base complement in the opposite orientation. Complementary inverted repeats have the potential to form hairpin loop or stem-loop structures which results in cruciform structures (such as CRUCIFORM DNA) when the complementary inverted repeats occur in double stranded regions.
Nucleic Acid Conformation
Aerosol Propellants
Compressed gases or vapors in a container which, upon release of pressure and expansion through a valve, carry another substance from the container. They are used for cosmetics, household cleaners, and so on. Examples are BUTANES; CARBON DIOXIDE; FLUOROCARBONS; NITROGEN; and PROPANE. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
rRNA Operon
RNA, Transfer, Ala
Transcription, Genetic
Bacterial Typing Techniques
RNA, Ribosomal, 5S
Aerosols
RNA, Bacterial
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
DNA Primers
Beclomethasone
Utility of internally transcribed 16S-23S rDNA spacer regions for the definition of Pseudomonas stutzeri genomovars and other Pseudomonas species. (1/1629)
Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA-DNA similarities. The internally transcribed 16S-23S rDNA spacer (ITS1) regions of P. stutzeri were analysed with respect to the ability of these nucleic acid regions to differentiate and identify the genomic groups (i.e. genomovars) of P. stutzeri. The ITS1s of 34 strains of P. stutzeri were amplified by PCR and the PCR product was subjected to RFLP analysis, which allowed the differentiation and identification of the strains to their respective genomovars. Sequence determination and analysis of ITS1s supported further the results obtained by RFLP, i.e. nucleotide signatures were identified in strains belonging to different genomovars. The ITS1s of all strains of P. stutzeri contained the tandem tRNA(Ile)/tRNA(Ala) genes and did not exhibit distinct sequence heterogeneity between different operons of a strain. Phylogenetically informative variable sites were located, exclusively, in non-coding regions. The results of the RFLP and sequence analysis of ITS1s supported and correlated with the phylogenetic relationships estimated from 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, offering an alternative tool for genomovar and species differentiation. (+info)High intraindividual variation in internal transcibed spacer sequences in Aeschynanthus (Gesneriaceae): implications for phylogenetics. (2/1629)
Aeschynanthus (Gesneriaceae) is a large genus of tropical epiphytes that is widely distributed from the Himalayas and China throughout South-East Asia to New Guinea and the Solomon Islands. Polymerase chain reaction (PCR) consensus sequences of the internal transcribed spacers (ITS) of Aeschynanthus nuclear ribosomal DNA showed sequence polymorphism that was difficult to interpret. Cloning individual sequences from the PCR product generated a phylogenetic tree of 23 Aeschynanthus species (two clones per species). The intraindividual clone pairs varied from 0 to 5.01%. We suggest that the high intraindividual sequence variation results from low molecular drive in the ITS of Aeschynanthus. However, this study shows that, despite the variation found within some individuals, it is still possible to use these data to reconstruct phylogenetic relationships of the species, suggesting that clone variation, although persistent, does not pre-date the divergence of Aeschynanthus species. The Aeschynanthus analysis revealed two major clades with different but overlapping geographic distributions and reflected classification based on morphology (particularly seed hair type). (+info)High-resolution phylogenetic analysis of NO2--oxidizing Nitrobacter species using the rrs-rrl IGS sequence and rrl genes. (3/1629)
A high-resolution phylogenetic analysis of Nitrobacter strains and their neighbours was made using the rrs-rrl intergenic spacer sequence and the hypervariable part of the rrl gene. The phylogenetic tree obtained was consistent with that which was obtained previously but was much more discriminating, permitting the design of genus-specific primers. (+info)Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae. (4/1629)
On the basis of genetic analysis, molecular karyotyping and sequence analyses of the 18S rRNA and internal transcribed spacer (ITS) region, three new Saccharomyces species are described, Saccharomyces cariocanus (with type strain NCYC 2890T), Saccharomyces kudriavzevii (with type strain NCYC 2889T) and Saccharomyces mikatae (with type strain NCYC 2888T). Genetic and molecular analyses did not confirm the previously observed conspecificity of Saccharomyces paradoxus and S. cariocanus. The latter species exhibits postzygotic isolation from representative strains from all known geographical populations of S. paradoxus: European, Far-East Asian, North American and Hawaiian. (+info)Molecular systematics of European Hyalodaphnia: the role of contemporary hybridization in ancient species. (5/1629)
We examined phylogenetic relationships among Daphnia using mitochondrial DNA (mtDNA) sequences from the small subunit ribosomal RNA (12S), cytochrome c oxidase subunit I and nuclear DNA sequences from the first and second internal transcribed spacer representing 1612 base positions. Phylogenetic analyses using several species of the three main Daphnia subgenera, Ctenodaphnia, Hyalodaphnia and Daphnia, revealed that the Hyalodaphnia are a monophyletic sister group of the Daphnia. Most Hyalodaphnia species occur on one continent, whereas only three are found in North America and Europe. Endemicity of species is associated with variation in thermal tolerance and habitat differentiation. Although many species of the Hyalodaphnia are known to hybridize in nature, mtDNA divergence is relatively high ca. 9%) compared to other hybridizing arthropods (ca. 3%). Reproductive isolation in Daphnia seems to evolve significantly slower than genetic isolation. We related these findings to what is known about the ecology and genetics of Daphnia in order to better understand the evolutionary diversification of lineages. The relationship of these data to phylogenetic patterns is discussed in the context of speciation processes in Daphnia. (+info)Detection and identification of mycobacteria by amplification of the internal transcribed spacer regions with genus- and species-specific PCR primers. (6/1629)
We evaluated the usefulness of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species level. The conservative and species-specific ITS sequences of 33 species of mycobacteria were analyzed in a multialignment analysis. One pair of panmycobacterial primers and seven pairs of mycobacterial species-specific primers were designed. All PCRs were performed under the same conditions. The specificities of the primers were tested with type strains of 20 mycobacterial species from the American Type Culture Collection; 205 clinical isolates of mycobacteria, including 118 Mycobacterium tuberculosis isolates and 87 isolates of nontuberculous mycobacteria from 10 species; and 76 clinical isolates of 28 nonmycobacterial pathogenic bacterial species. PCR with the panmycobacterial primers amplified fragments of approximately 270 to 400 bp in all mycobacteria. PCR with the M. tuberculosis complex-specific primers amplified an approximately 120-bp fragment only for the M. tuberculosis complex. Multiplex PCR with the panmycobacterial primers and the M. tuberculosis complex-specific primers amplified two fragments that were specific for all mycobacteria and the M. tuberculosis complex, respectively. PCR with M. avium complex-, M. fortuitum-, M. chelonae-, M. gordonae-, M. scrofulaceum-, and M. szulgai-specific primers amplified specific fragments only for the respective target organisms. These novel primers can be used to detect and identify mycobacteria simultaneously under the same PCR conditions. Furthermore, this protocol facilitates early and accurate diagnosis of mycobacteriosis. (+info)Divergent mechanisms of 5' 23S rRNA IVS processing in the alpha-proteobacteria. (7/1629)
Widespread occurrence of a separate small RNA derived from the 5'-end of 23S rRNA and of an intervening sequence (IVS) which separates this domain from the main segment of 23S rRNA in the alpha-proteobacteria implies that processing reactions which act to excise the IVS are also maintained in this group. We previously characterized the first example of processing of this IVS in Rhodopseudomonas palustris, which is classified with the Bradyrhizobia In this case, IVS excision occurs by a multistep process and RNase III appears to act at an early step. Here, we characterize in vivo and in vitro IVS processing in two other related, but phenotypically distinct, Bradyrhizobia We also examine in vivo and in vitro processing of rRNA precursors from a more distantly related alpha-proteobacterium, Rhodobacter sphaeroides which produces a separate 5' 23S rRNA domain but has different sequences in the 5' 23S rRNA IVS. The details of the in vivo processing of all of the Bradyrhizobial rRNAs closely resemble the R. palustris example and in vitro studies suggest that all of the Bradyrhizobia utilize RNase III in the first step of IVS cleavage. Remarkably, in vivo and in vitro studies with R.sphaeroides indicate that initial IVS cleavage uses a different mechanism. While the mechanism of IVS cleavage differs among these alpha-proteobacteria, in all of these cases the limits of the internal segments processed in vivo are almost identical and occur far beyond the initial cleavage sites within the IVSs. We propose that these bacteria possess common secondary maturation pathways which enable them to generate similarly processed 23S rRNA 5'- and 3'-ends. (+info)Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. (8/1629)
Trichophyton rubrum is the commonest cause of dermatophytosis of skin and nail tissue. Molecular characterization of the T. rubrum ribosomal DNA nontranscribed-spacer region revealed two novel tandemly repetitive subelements (TRSs): TRS-1, containing a 27-bp palindromic sequence, and TRS-2. Specific amplification of TRS-1 produced strain-characteristic banding patterns (PCR types), with 21 TRS-1 PCR types recognized from 101 clinical isolates. Four simple patterns representing 1 to 4 copies of TRS-1 accounted for 75 (75%) of all 101 strains, whereas more complex patterns were observed for 21 (20%) of the 101 isolates. The copy number of TRS-2 was 0 to 3 repeats per cistron, with a majority of isolates having two copies of this element. Eleven isolates were polymorphic for TRS-2, and in combination, 23 separate PCR types were recognized by amplification of both TRS-1 and TRS-2. The PCR patterns from both elements were stable and reproducible. Elements with homology to TRS-1 were present in three phylogenetically related species, Trichophyton violaceum, Trichophyton gourvilii, and Trichophyton soudanense, but these elements were not identified in other dermatophyte taxa. There was no clear correlation of PCR type with specimen (skin or nail tissue), but certain PCR types appeared to show a bias in geographic distribution. This new method of typing T. rubrum will enable important questions about pathogenesis and epidemiology of this fungus to be addressed. (+info)
Automated Ribosomal Intergenic Spacer Analysis (ARISA) - Science Exchange
Comparative analysis of the genes encoding 23S-5S rRNA intergenic spacer regions of Lactobacillus casei-related strains |...
PCR Targeted to the 16S-23S rRNA Gene Intergenic Spacer Region ofClostridium difficile and Construction of a Library Consisting...
Identification of virulence factors in 16S-23S rRNA intergenic spacer genotyped Staphylococcus aureus isolated from water...
3M Membrane Switch Spacers for Marine | 3M United States
NZResearch.org
ecoLincNZ: Bacteria, friend not foe in stream ecology
Microbial Community Structure in Hydrocarbon Impacted Sediment Associa by Joseph W. Duris
Patent US5203108 - Method and device for heat killing insects in bulk produce containers - Google Patents
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Postangiography patients who complete cardiac rehabilitation have reduced risk of mortality or hospitalisation compared to non...
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Phylogeny of Porphyromonas gingivalis by Ribosomal Intergenic Spacer Region Analysis | Journal of Clinical Microbiology
Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi | PNAS
Rapid detection of emerging pathogens and loss of microbial diversity associated with severe lung disease in cystic fibrosis ...
matK-QR classifier: a patterns based approach for plant species identification | BioData Mining | Full Text
Report of the second internal workshop on human chromosome 5 mapping<...
Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for...
Spacers For Your Teeth
Are Toyota bZ4X Wheel Spacers Safe for Daily Driving? - BONOSS
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Leishmania infantum
Kuhls K, Mauricio IL, Pratlong F, Presber W, Schönian G (2005). "Analysis of ribosomal DNA internal transcribed spacer ... The smaller size of the Leishmania BRCA2 DNA repair protein has been exploited to better understand its function in homologous ...
Fabaceae
Evidence from nuclear ribosomal DNA internal transcribed spacer sequences". American Journal of Botany. 80 (6): 711-722. doi: ... or the ribosomal spacers ITS) and cladistic analysis in order to investigate the relationships between the family's different ... Lavin M, Doyle JJ, Palmer JD (1990). "Evolutionary significance of the loss of the chloroplast-DNA inverted repeat in the ... These studies have used morphology, DNA data (the chloroplast intron trnL, the chloroplast genes rbcL and matK, ...
Acytostelium
5.8S ribosomal DNA and internal transcribed spacer region analyses". Eukaryotic Cell. 6 (1): 110-6. doi:10.1128/EC.00233-06. ...
Lomentospora prolificans
1: 3-9. Lennon, PA; Cooper CR; Salkin IF; Lee SB (1994). "Ribosomal DNA internal transcribed spacer analysis supports synonymy ... This synonymy has since been confirmed by phylogenetic analysis of the nuclear ribosomal internal transcribed spacer regions. ... of cultures of Scedosporium-like fungi from clinical cases by careful morphological examination and the evaluation of DNA-DNA ...
List of Astragalus species
Evidence from Nuclear Ribosomal DNA Internal Transcribed Spacer Sequences". Am J Bot. 80 (6): 711-722. doi:10.2307/2445441. ... Phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndhF sequences". Plant Syst ... and its Major Subgroups Based on Nuclear Ribosomal DNA ITS and Chloroplast DNA trnL Intron Data". Syst Bot. 24 (3): 409-437. ... molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA". J Agric Food Chem. 51 (23): 6709-6714. doi: ...
Erysiphales
... inferred from internal transcribed spacer ribosomal DNA sequences". Canadian Journal of Botany. 77 (1): 150-168. doi:10.1139/ ...
Rubus
... based on nuclear ribosomal DNA internal transcribed spacer region sequences". American Journal of Botany. Botanical Society of ...
Robert W. Allard
Saghai-Maroof, M.A.; Soliman, K.M.; Jorgensen, R.A. (1984). "Ribosomal DNA spacer-length polymorphisms in barley: mendelian ... Cluster, P.D.; Allard, R.W. (1995). "Evolution of ribosomal DNA (rDNA) genetic structure in colonial Californian populations of ... Wagner, D.B.; Furnier, G.R.; Saghai-Maroof, M.A.; Williams, S.M.; Dancik, B.P.; Allard, R.W. (1987). "Chloroplast DNA ... Neale, D.B.; Saghai-Maroof, M.A.; Allard, R.W.; Zhang, Q.; Jorgensen, R.A. (1988). "Chloroplast DNA diversity in populations of ...
Conserved sequence
"Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi". Proceedings of the ... Highly conserved DNA sequences are thought to have functional value, although the role for many highly conserved non-coding DNA ... such as ribosomal RNAs, tRNAs and ribosomal proteins are also universally conserved. Sets of conserved sequences are often used ... "Characterization of Bacterial and Fungal Soil Communities by Automated Ribosomal Intergenic Spacer Analysis Fingerprints: ...
Molecular ecology
Kiss L (July 2012). "Limits of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as species barcodes for Fungi ... The amplification of DNA from environmental samples using general or group-specific primers leads to a mix of genetic material ... 2013), the D1-D3 region of 28S ribosomal RNA genes, may not allow the low taxonomic level classification of the ITS, but ... The development of molecular ecology is also closely related to the use of DNA microarrays, which allows for the simultaneous ...
RNR1
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ... 1985). "Molecular analysis of the heterogeneity region of the human ribosomal spacer". J. Mol. Biol. 183 (2): 213-223. doi: ...
RNR3
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ... 1985). "Molecular analysis of the heterogeneity region of the human ribosomal spacer". J. Mol. Biol. 183 (2): 213-223. doi: ...
Daniel Lee Nickrent
... based on nuclear ribosomal DNA internal transcribed spacer sequences". American Journal of Botany. 81 (9): 1149-1160. doi: ... During this period, his laboratory methodology changed from working with rRNA to DNA using the polymerase chain reaction (PCR) ...
RNR5
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... RNA, ribosomal 5, also known as RNR5, is a human gene. Genes for ribosomal RNA are clustered on the short arms of chromosomes ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ...
RNR4
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ... 1985). "Molecular analysis of the heterogeneity region of the human ribosomal spacer". J. Mol. Biol. 183 (2): 213-223. doi: ...
Hoplolaimus
... using the first internal transcribed spacer and the D1-D3 expansion segments of 28S ribosomal DNA. Journal of Nematology 40(3 ...
Rebecca Yahr
2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of ...
Nirarathamnos
... evidence from nuclear ribosomal DNA internal transcribed spacer sequences" (PDF). American Journal of Botany. 87 (1): 76-95. ...
Beryl B. Simpson
High-resolution phylogeny for Helianthus (Asteraceae) using the 18S-26S ribosomal DNA external transcribed spacer. American ...
Prunus × nudiflora
... based on internal transcribed spacer sequences of ribosomal DNA". Korean Journal of Genetics. 20 =issue=2: 109-116. The idea ... conducted the phylogenetic analysis using sequences from both rbcL gene and trnL-trnF intergenic spacer of chloroplast DNA and ... September 2002). "Phylogenetic Analysis of Korean Prunus ( Rosaceae ) Based on ITS Sequences of Nuclear Ribosomal DNA". Gene & ... markers and sequence analysis of two chloroplast DNA genes, rpl16 and trnL-trnF spacer and showed that king cherry can be ...
Phoebanthus
Phylogenetic relationships in Helianthus (Asteraceae) based on nuclear ribosomal DNA internal transcribed spacer region ...
Sarcoscypha coccinea
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... Her cladistic analysis combined comparisons of the sequences of the internal transcribed spacer in the non-functional RNA with ...
Hamamelidaceae
... of nuclear ribosomal DNA". American Journal of Botany. 86 (7): 1027-1037. doi:10.2307/2656620. JSTOR 2656620. PMID 10406726. Li ... "Phylogenetic relationships of the Hamamelidaceae inferred from sequences of internal transcribed spacers (ITS) ... "Phylogeny of the Hamamelidaceae based on the ITS sequences of nuclear ribosomal DNA". Biochemical Systematics and Ecology. 26 ( ... Morphological and DNA studies have supported monophyly of the Hamamelidoideae and have recognized the separation of the ...
Rottboellia cochinchinensis
Based on Nuclear Ribosomal Internal Transcribed Spacer and Chloroplast trnL-F Sequences. In Aliso: A Journal of Systematic and ... based on chloroplast and nuclear DNA sequences. Telopea, 13(1-2), 115-142. Soreng, R. J., Peterson, P. M., Romaschenko, K., ...
Sarcoscypha occidentalis
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... The cladistic analysis combined comparison of sequences from the internal transcribed spacer in the non-functional RNA with ...
Phalaenopsis pantherina
... based on the internal transcribed spacer of the nuclear ribosomal DNA. Plant Systematics and Evolution, 256(1), 1-16. Tsai, C. ...
Sarcoscypha dudleyi
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... The cladistic analysis combined comparison of sequences from the internal transcribed spacer in the non-functional RNA with ...
Antonius Suwanto
... of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer ... DNA sequence and mechanism of transfer". Journal of Bacteriology. 182 (1): 81-90. doi:10.1128/jb.182.1.81-90.2000. PMC 94243. ...
Lupinus
... based on internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA" (PDF). American Journal of Botany. 86 (4): 590- ... 2005). 2C DNA variation and relationships among New World species of the genus Lupinus (Fabaceae). Plant Systematics and ...
Mycobacterium senegalense
Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer ...
Genome size
... genome sizes Human genome Junk DNA List of sequenced eukaryotic genomes Non-coding DNA Plant DNA C-values Database Selfish DNA ... "Psyllid endosymbionts exhibit patterns of co-speciation with hosts and destabilizing substitutions in ribosomal RNA". Insect ... dispersed in the ancestor gene but aggregated and the negative relation between number of lost genes and length of the spacers ... Some single-celled organisms have much more DNA than humans, for reasons that remain unclear (see non-coding DNA and C-value ...
Acis rosea
... based on plastid matK and nuclear ribosomal spacer (ITS) DNA sequences and morphology", Plant Systematics and Evolution, 246 (3 ...
Crinipellis zonata
The conclusions of this analysis, based on the DNA sequences of ribosomal DNA coding for internal transcribed spacers, are ...
List of homing endonuclease cutting sites
"Six group I introns and three internal transcribed spacers in the chloroplast large subunit ribosomal RNA gene of the green ... They act on the cellular DNA of the cell that synthesizes them; to be precise, in the opposite allele of the gene that encode ... Three internal transcribed spacers and 12 group I intron insertion sites". J Mol Biol. 232 (2): 446-67. doi:10.1006/jmbi. ... Turmel M, Mercier JP, Côté V, Otis C, Lemieux C (July 1995). "The site-specific DNA endonuclease encoded by a group I intron in ...
Ubiquitin
Hofmann K (April 2009). "Ubiquitin-binding domains and their role in the DNA damage response". DNA Repair. 8 (4): 544-56. doi: ... UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The UBB ... The UBDs exploit this by having small spacers between ubiquitin-interacting motifs that bind lysine 48-linked chains (compact ... Monoubiquitinated PCNA recruits polymerases that can carry out DNA synthesis with damaged DNA; but this is very error-prone, ...
Astragalus fragrans
... inferred from nuclear ribosomal DNA Internal transcribed spacers data (nrDNA ITS)". Ecologia Mediterranea. 36 (1): 99-106. doi: ...
Galerina marginata
... five represented the same species after comparing the DNA sequences of the internal transcribed spacer region of ribosomal DNA ... marginata based on ribosomal DNA sequences and restriction fragment length polymorphism analyses. Because of differences in ... The amatoxins inhibit the enzyme RNA polymerase II, which copies the genetic code of DNA into messenger RNA molecules. The ... List of deadly fungi Gulden G, Dunham S, Stockman J (2001). "DNA studies in the Galerina marginata complex". Mycological ...
MicroRNA
Pre-mRNA sequence of miR-712 is generated from the murine ribosomal RN45s gene at the internal transcribed spacer region 2 ( ... Defects in DNA repair cause the accumulation of mutations, which can lead to cancer. Several genes involved in DNA repair are ... Cancer is caused by the accumulation of mutations from either DNA damage or uncorrected errors in DNA replication. ... DNA and Cell Biology. 26 (4): 209-18. doi:10.1089/dna.2006.0545. PMID 17465887. Peterson KJ, Dietrich MR, McPeek MA (July 2009 ...
Phalaenopsis javanica
Based on Gen Internal Transcribed Spacer (ITS1 AND ITS2), 5.8 DNA Ribosomal. In Proceeding International Conference on Global ...
Korarchaeota
Achenbach-Richter, L; Woese CR (1988). "The ribosomal gene spacer region in archaebacteria". Syst. Appl. Microbiol. 10 (3): 211 ... "DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA". Int. J. Syst. Evol. Microbiol. 50 (3): 1095- ... Winker, S; Woese CR (1991). "A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA ... Hansmann, S; Martin W (2000). "Phylogeny of 33 ribosomal and six other proteins encoded in an ancient gene cluster that is ...
Dryas (plant)
... of Nuclear Ribosomal DNA and the trnL/F Region of Chloroplast DNA" (PDF), International Journal of Plant Sciences, 164 (2): 197 ... 2003), "The Phylogeny of Rosoideae (Rosaceae) Based on Sequences of the Internal Transcribed Spacers (ITS) ...
Streptocarpus
... based on ribosomal DNA internal transcribed spacer (ITS) sequences", American Journal of Botany, 84 (7): 956-965, doi:10.2307/ ... DNA studies have shown that, despite not having a twisted fruit, African violets evolved from within the Tanzanian ...
Acis valentina
... based on plastid matK and nuclear ribosomal spacer (ITS) DNA sequences and morphology", Plant Systematics and Evolution, 246 (3 ...
Cunninghamella bertholletiae
Recently, DNA barcoding of the internal transcribed spacer (ITS) region of C. bertholletiae ribosomal DNA was performed to ... "DNA barcoding of clinically relevant Cunninghamella species". Medical Mycology. 53 (2): 99-106. doi:10.1093/mmy/myu079. PMID ...
Artemisia (plant)
... based on sequences from the internal transcribed spacers (ITS) of nuclear ribosomal DNA". American Journal of Botany. 85 (12): ... after analyzing and matching the internal transcribed spacers of nuclear ribosomal DNA from many Seriphidium and Artemisia ... Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS)". Plant Biology. 5 (3): 274-84. doi: ... Chloroplast and ribosomal DNA sequence analysis in 2011 supported monophyly with two clades, one of which includes some North ...
Sugarcane grassy shoot disease
The ribosomal DNA contains one transcriptional unit with a cluster of genes coding for the 18S, 5.8S and 28S rRNAs and two ... Identification of phytoplasma strain-specific PCR primers obtained from 16S/23S rRNA spacer sequences. (1996). Appl. Environ. ... Though reliable, DNA hybridization, electron microscopy and PCR techniques require specialized equipment and trained human ... Ahrens, U., and Seemüller, E. (1992) Detection of DNA of plant pathogenic mycoplasma-like organisms by a polymerase chain ...
Cattleya crispa
Based on Sequence Data from Internal Transcribed Spacers (ITS) of Nuclear Ribosomal DNA", Lindleyana 15(2): 96-114. 2000. The ...
Monarda
... region of nuclear ribosomal DNA", Systematic Botany, 27 (1): 127-137, doi:10.1043/0363-6445-27.1.127 (inactive 31 July 2022){{ ... 2002), "Monophyly and phylogeny of Monarda (Lamiaceae): Evidence from the internal transcribed spacer (ITS) ...
Paeonia mairei
... using internal transcripted spacer sequences of nuclear ribosomal DNA: Implications for biogeography and concerted evolution". ...
Bromus lanceolatus
Insights from internal transcribed spacer sequences of nuclear ribosomal DNA". Genome. 40 (5): 730-743. doi:10.1139/g97-796. ...
Deoxyribozyme
In one application an artificial DNA catalyst was prepared by attaching a copper ion to it through a spacer. The copper - DNA ... autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena". Cell. 31 (1): 147-157. doi:10.1016/ ... Other uses of DNA in chemistry are in DNA-templated synthesis, Enantioselective catalysis, DNA nanowires and DNA computing. ... Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of performing a ...
Ficus rubiginosa
Nina Rønsted and colleagues analysed the DNA sequences from the nuclear ribosomal internal and external transcribed spacers, ...
Split gene theory
This was not known, as the distribution of ORF lengths in a random DNA sequence had never been studied. As random DNA sequences ... The theory correctly predicts that splice junctions present in transfer RNA genes and ribosomal RNA genes, do not contain stop ... In their view, introns originated as spacer sequences that enabled convenient recombination and shuffling of exons that encoded ... If split genes originated from random primordial DNA sequences, they would contain a significant amount of DNA that represented ...
Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi<...
Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. In: Proceedings of the ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. / Schoch, Conrad L.; ... title = "Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi", ...
The opportunistic yeast pathogen Trichosporon asahii colonizes the skin of healthy individuals: analysis of 380 healthy...
DeCS - Termos Novos
DNA CATALÍTICO. DNA, CATALYTIC. ADN CATALITICO. DNA ESPAÇADOR RIBOSSÔMICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN ... DNA INTERGÊNICO. DNA, INTERGENIC. ADN INTERGENICO. DOENÇAS TRANSMISSÍVEIS EMERGENTES. COMMUNICABLE DISEASES, EMERGING. ... ENSAIO DE AMPLIFICAÇÃO DE SINAL DE DNA RAMIFICADO. BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ...
Science Clips - Volume 13, Issue 26, July 27, 2021
Definitive identification was achieved using the ribosomal DNA (rDNA) sequence analysis by targeting the ITS1 (internal ... transcribed spacer 1) region. This case report is intended to promote awareness of this fungus as a potential pathogen, by ... mitochondrial DNA genomes inferred two clades of plasmodia in orangutans (Pongo 1 and Pongo 2), and that these clades likely ... Of all biospecimen types reported in the literature, the most frequently used were DNA, postmortem spinal cord, blood, and ...
Assessment of microbiological contamination in the work environments of museums, archives and libraries | SpringerLink
Rapid identification of bacteria on the basis polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Applied ... Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of ... White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for ... Halstensen, A. S. (2008). Species-specific fungal DNA in airborne dust as surrogate for occupational mycotoxin exposure. ...
Subject term: dieback - PubAg Search Results
... internal transcribed spacers; mycelium; pathogenicity; pathogens; peptide elongation factors; ribosomal DNA; sand; soil; spring ... Camellia sinensis; DNA-directed RNA polymerase; Fusarium solani; air drying; autoclaving; bark; conidia; culture media; ... DNA; Ficus carica; Lasiodiplodia theobromae; alcohols; ambient temperature; cell walls; conidia; culture media; data collection ... DNA; Neoscytalidium dimidiatum; Olea europaea; Turkey (country); bark; conidia; cultivars; dieback; disease control; disease ...
T. Garnatje | Semantic Scholar
Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS). *J. Vallès, M. Torrell, T. Garnatje ... A flow cytometric study of nuclear DNA content in a wide range of taxa of the Asteraceae family is carried out, filling gaps in ... THE CARDUEAE (COMPOSITAE) REVISITED: INSIGHTS FROM ITS, trnL-trnF, AND matK NUCLEAR AND CHLOROPLAST DNA ANALYSIS1, 2. *A. ... Generic Delimitation and Phylogeny of the Subtribe Centaureinae (Asteraceae): A Combined Nuclear and Chloroplast DNA Analysis. ...
Volume 54 Issue 6 | Journal of Medical Entomology
In this study, we analyzed the Leishmania DNA using nuclear ribosomal internal transcript spacer (ITS) sequences. Parasite DNA ... DNA of R. parkeri was detected in a total of 44 (24%) of these ticks. DNA of "Candidatus Rickettsia andeanae" and Rickettsia ... In addition, we developed a simple protocol to extract DNA from sonicated first-instar larvae, and used that DNA to test the ... The lower limit of detection for our assay is equivalent to ~0.03 of a first-instar larva in a 60-μl sample (0.016 ng of DNA ...
Rubus in Flora of North America @ efloras.org
Trichosporon Infections: Background, Pathophysiology, Epidemiology
Trichosporon asahii genotypes determined on the basis of ribosomal DNA intergenic spacer 1 sequencing, and antifungal ... Invasive Trichosporon infection in solid organ transplant patients: a report of two cases identified using IGS1 ribosomal DNA ... DNA micro-array based detection and identification of fungal pathogens in clinical samples from neutropenic patients. J Clin ...
WHO EMRO | Insecticide susceptibility status of the malaria vector Anopheles arabiensis in Khartoum city, Sudan: differences...
PCR method was based on specific DNA nucleotide differences in the intergenic spacer of ribosomal DNA (rDNA) [11]. The ... The DNA was extracted from a single mosquito using a DNA extraction kit (Qiagen) with 1 uL of the DNA amplified in a 24 uL PCR ... and 0.9 U DNA polymerase (AmpliTaq). The PCR condition with an initial step of 10 min at 94°C, followed by 30 cycles each ...
Talaromyces marneffei (Segretain et al.) Samson et al. - 64101 | ATCC
Bacillary Angiomatosis Workup: Approach Considerations, Procedures, Laboratory Studies
... assay or of Bartonella antigens targeting the 16S-23S ribosomal RNA gene intergenic transcribed spacer via immunohistochemical ... 4] Note that a negative result suggests the absence of detectable DNA but does not rule out Bartonella infection. The PCR assay ... Detection of Bartonella DNA in tissue specimens via polymerase chain reaction (PCR) ... Multiplexed colorimetric detection of Kaposis sarcoma associated herpesvirus and Bartonella DNA using gold and silver ...
Forest Research Institute Malaysia (FRIM) | Kuala Lumpur, Malaysia | frim
... and ITS4 regions of nuclear ribosomal DNA. A total of 66 endophytic fungi were successfully isolated from different parts of A ... trees based on the concatenated CDS and internal transcribed spacer (ITS) sequences that were analyzed separately have revealed ... Endophytic fungal identification was conducted by PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) ...
NIOSHTIC-2 Search Results - Basic View
... yeasts in floor dust of 50 elementary schools characterized with sequencing internal transcribed spacer region of ribosomal DNA ... Terms: DNA or Deoxyribonucleic acid OR DNA or Deoxyribonucleic acid 1 - 10 of 581 Bibliographic entries ... Global DNA methylation of WTC prostate cancer tissues show signature differences compared to non-exposed cases. ... Global DNA methylation profiles in peripheral blood of WTC-exposed community members with breast cancer. ...
Molecular Characteristics of Camallanus Spp. (Spirurida: Camallanidae) in Fishes from China Based On its rDNA Sequences |...
Apidologie
1999 AES Annual Meeting Abstracts - AES | American Elasmobranch Society
Characterization of group I intron-like insertion elements in shark ribosomal DNA spacers.. ... A set of insertion elements has been found in the ribosomal DNA (rDNA) internal transcribed spacers in four Orders of sharks ( ... The use of PCR-RFLP and DNA sequence analysis of major histocompatibility class II alpha genes for paternity testing in the ... Microsatellite DNA profiling in the blue shark Prionace glauca : development and potential application for population studies. ...
Journal of Medicinal Plants Research - plant-specific primers for the amplification of the nrdna its region in fungus...
... region of nuclear ribosomal DNA is the most frequently used marker for distinguishing plant species. Previously published ... region of nuclear ribosomal DNA is the most frequently used marker for distinguishing plant species. Previously published ... could not preferentially amplify plant DNA or discriminate against fungal DNA. For P. tongkangensis, the dismatch of ITS5 ... could not preferentially amplify plant DNA or discriminate against fungal DNA. For P. tongkangensis, the dismatch of ITS5 ...
Development of ITS based SCAR Markers for Some Medicinally Important Species of Phyllanthus
Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA. Theor. Applied ... Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA in Winteraceae. Am ... DNA amplification profile using PE [F and R] primers. Lanes M-100 bp DNA ladder, PL-Phyllanthus emblica DNA from leaves. PF- ... DNA extraction, ITS amplification and sequencing: Total genomic DNA was isolated from leaf materials following the DNA ...
Bartonella rochalimae and Other Bartonella spp. in Fleas, Chile - Volume 15, Number 7-July 2009 - Emerging Infectious Diseases...
The nucleotide sequence of the 16S-23S ribosomal RNA intergenic spacer obtained from 1 genotype (clone F17688) was nearly ... 9) amplified Bartonella DNA by using PCR with Pulex spp. fleas collected from persons in Peru and suggested the existence of a ... Each flea was crushed with a sterile pestle, and DNA was extracted by lysis with 0.7 M ammonium hydroxide. PCR was used to ... sterile water instead of template DNA) were used. PCR products were purified, and both strands of each amplicon were subjected ...
Publication : USDA ARS
Publications
Resolution of Prochlorococcus and Synechococcus ecotypes by using 16S-23S ribosomal DNA internal transcribed spacer sequences. ... DNA base composition and genome size of the prokaryotic symbiont in Riftia pachyptila (Pogonophora). FEMS Microbiol. Let. 24: ... Trichodesmium genome maintains abundant, widespread noncoding DNA in situ, despite oligotrophic lifestyle. PNAS 112: 4251-4256 ...
PCR-based molecular identification of two intermediate snail hosts of Schistosoma mansoni in Cameroon | Parasites & Vectors |...
... region of Biomphalaria ribosomal DNA (rDNA) using polymerase chain reaction amplification (PCR) and restriction fragment length ... Kane RA, Rollinson D. Repetitive sequences in the ribosomal DNA internal transcribed spacer of Schistosoma haematobium, ... We analyzed the internal transcribed spacer 2 (ITS2) region of Biomphalaria ribosomal DNA (rDNA) using polymerase chain ... reinvestigated using nuclear ribosomal DNA internal transcribed spacer sequences. Can J Zool. 1997;75:1540-5. ...
Frontiers | SMRT Sequencing Revealed Mitogenome Characteristics and Mitogenome-Wide DNA Modification Pattern in Ophiocordyceps...
In addition, the mt genome-wide base modification pattern of O. sinensis was determined in this study, especially DNA ... In addition, the mt genome-wide base modification pattern of O. sinensis was determined in this study, especially DNA ... genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt ... genome of Ophiocordycpes sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt ...
Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU...
Complex communities of fungi are regularly characterised by metabarcoding using the Internal Transcribed Spacer (ITS) and the ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the ... There is broad agreement that the internal transcribed spacer (ITS) of the nuclear rRNA gene cluster should be the standard DNA ... Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several ...
British Library EThOS: Phylogeny, taxonomy and biogeography of Ceiba Mill. (Malvaceae: Bombacoideae)
Chromatin and siRNA pathways cooperate to maintain DNA methylation of small transposable elements in Arabidopsis | Genome...
Our findings also suggest that RNA-directed DNA methylation is required to silence isolated elements that may be too small to ... In Arabidopsis, DNA cytosine methylation is maintained by three subfamilies of methyltransferases with distinct substrate ... We used a microarray-based profiling method to explore the involvement of Arabidopsis CMT3 and DRM DNA methyltransferases, a ... We conclude that the targets of both DNA methylation and histone H3K9 methylation pathways are transposable elements genome- ...
RDNA8
- We determined the internal transcribed spacers of ribosomal DNA (ITS rDNA) sequences of these nematodes. (allenpress.com)
- Studies of restriction site variation in rDNA in populations of animals and plants have shown that while coding regions are conserved, these spacer regions are variable ( Gerbi,1985 ). (scialert.net)
- We analyzed the internal transcribed spacer 2 (ITS2) region of Biomphalaria ribosomal DNA (rDNA) using polymerase chain reaction amplification (PCR) and restriction fragment length polymorphism (RFLP). (biomedcentral.com)
- We reidentify three rare yeasts from a culture collection of onychomycosis agents by matrix-assisted laser desorption/ionization time of flight/mass spectrometry (MALDI-TOF/MS) and sequencing the internal transcribed spacer (ITS) regions or the intergenic spacer (IGS) 1 region of ribosomal DNA (rDNA), and present their enzymatic and antifungal susceptibility profiles. (em-consulte.com)
- We analyse intragenomic variation of the ITS2 internal transcribed spacer of ribosomal DNA (rDNA) in the grasshopper Eyprepocnemis plorans, by means of tagged PCR 454 amplicon sequencing performed on both genomic DNA (gDNA) and RNA-derived complementary DNA (cDNA), using part of the ITS2 flanking coding regions (5.8S and 28S rDNA) as an internal control for sequencing errors. (umn.edu)
- We studied the organization of rDNA spacer sequences within several rDNA loci in D. melanogaster. (elsevier.com)
- Every locus showed many discrete length classes of rDNA spacer, ranging from 2.4 kb to about 20 kb. (elsevier.com)
- Only a small fraction of a typical eukaryotic nuclear genome constitutes rDNA or protein-coding genes, whereas a large fraction of all higher eukaryotic genomes consists of repetitive sequences and heterochromatic satellite DNA (satDNA) [ 1 , 2 ]. (biomedcentral.com)
Intergenic spacer region2
- The sequencing of the 16S-23S ribosomal RNA intergenic spacer region (ITS) showed that all bacteria belonged to the Bradyrhizobium genus. (springernature.com)
- Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. (bvsalud.org)
Sequences10
- Internal transcribed spacer regions are sequences located in eukaryotic rRNA genes between the 18S and 5.8S rRNA coding regions (ITS1) and between the 5.8S and 25S (26S) rRNA coding regions (ITS2). (scialert.net)
- In fungi and plants, however, the localized nature of DNA methylation makes it possible to identify sequences that are targeted for DNA methylation. (biomedcentral.com)
- For example, in Neurospora , DNA methylation occurs at repeated sequences that are targeted for point mutation [ 5 ]. (biomedcentral.com)
- The Dnmt3 subfamily of DNA methyltransferases, which includes Arabidopsis DRM1 and DRM2, can methylate de novo [ 9 ], but there are no known sequences in common among target sites. (biomedcentral.com)
- The CV method performs well in grouping effectiveness of DNA barcode sequences, as compared to K2P analysis of aligned sequences. (biomedcentral.com)
- Species were identifi ed on the basis of source of infection by entities emerging on the human internal transcribed spacer (ITS), partial cell division cycle host is essential for understanding the evolution of ( CDC42 ), -tubulin ( BT2 ), and ACT sequences ( 10-14 ). (cdc.gov)
- The hyrcanus group of Anopheles ( Anopheles ) in China (Diptera: Culicidae): Species discrimination and phylogenetic relationships inferred by ribosomal DNA internal transcribed spacer 2 sequences. (si.edu)
- Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses. (psu.ac.th)
- DNA sequences may be stored in databases accessible over the internet, obviating the need for the exchange of reference strains. (biomedcentral.com)
- Typing procedures based on DNA sequences overcome these limitations, since sequence data may easily be exchanged and stored in databases that are accessible via the internet. (biomedcentral.com)
Nuclear ribosomal large subunit2
- The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. (illinois.edu)
- In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. (edu.hk)
Subunit7
- Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). (illinois.edu)
- The nuclear ribosomal small subunit has poor species-level resolution in fungi. (illinois.edu)
- Ceballos-Escalera A, Richards J, Arias MB, Inward DJG, Vogler AP (2022) Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU) rRNA markers. (pensoft.net)
- Complex communities of fungi are regularly characterised by metabarcoding using the Internal Transcribed Spacer ( ITS ) and the Large-Subunit ( LSU ) gene of the rRNA locus, but reliance on a single short sequence fragment limits the confidence of identification. (pensoft.net)
- In 2003, Hebert et al [ 1 ] proposed to use a 648-bp region from the 5'-end of the cytochrome c oxidase subunit 1 (COI) gene as a DNA barcode for identifying all metazoan species. (biomedcentral.com)
- We evaluated the systematics of these taxa with phylogenetic analyses of DNA sequence data from the nuclear ribosomal regions of the internal transcribed spacer (ITS) and 28S large subunit (LSU). (northwestern.edu)
- Based on morphology and DNA sequence data of the large subunit nuclear ribosomal RNA gene (LSU, 28S) and the internal transcribed spacers (ITS) and 5.8S rRNA gene of the nrDNA operon, the genus Tiarosporella is shown to be poly- and paraphyletic. (up.ac.za)
Gene6
- Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. (illinois.edu)
- The absence of all potential vectors of human Plas- an internal transcribed spacer 2 gene sequence analysis modium spp. (cdc.gov)
- Detection of Bartonella DNA in tissue specimens via polymerase chain reaction (PCR) assay or of Bartonella antigens targeting the 16S-23S ribosomal RNA gene intergenic transcribed spacer via immunohistochemical methods is diagnostic. (medscape.com)
- DSB-repair within the ribosomal RNA tandem gene-repeats is likely dominated by single-strand annealing allowing approximately 80% of cells to survive the break. (pasteur.fr)
- Our goal was to clarify ancestral relationships, investigate the position of Dalibarda repens relative to Rubus, and examine the origin of the circumboreal, octoploid species R. chamaemorus using sequence data from one additional chloroplast DNA region, trnS-trnG, and the singlecopy nuclear gene Granule-Bound Starch Synthase (GBSSI-1). (wku.edu)
- We cloned and sequenced 18S ribosomal DNA and the internal transcribed spacer-1 (ITS-1) gene. (marquette.edu)
Fungal5
- Endophytic fungal identification was conducted by PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) and ITS4 regions of nuclear ribosomal DNA. (researchgate.net)
- Previously published polymerase chain reaction (PCR) primers available for amplifying the ITS region from environmental plant samples provide a high degrees of success while maintaining a broad range of compatibility difficult to discriminate against fungal DNA. (academicjournals.org)
- Thus, the plant-specific ITS primer design is required to achieve the preferential amplification of plant DNA with discrimination against fungal DNA from environmental plant samples. (academicjournals.org)
- Our results showed that ITS4-R, when paired with either a universal primer ITS5 or the newly designed primer ITS1-F2, efficiently amplified DNA from environmental P. cernua samples and discriminated against parasitic fungal DNAs, while another newly designed primer ITS1-F2, when paired with either a universal primer ITS4 or ITS4-R, could not preferentially amplify plant DNA or discriminate against fungal DNA. (academicjournals.org)
- For P. tongkangensis , the dismatch of ITS5 primer resulted in noneffective or wrong amplification when paired with either ITS4-R or ITS4, while ITS1-F2, when paired with ITS4-R and ITS4, efficiently amplified plant DNA and both plant and fungal DNAs, respectively. (academicjournals.org)
Amplification1
- COI has several claims to be a suitable DNA barcode marker, including ease in amplification across a wide variety of organisms and provision of enough information to enable organisms to be identified to the species level. (biomedcentral.com)
Isolates4
- Multigene phylogenies (internal transcribed spacer ribosomal DNA, ß-tubulin, and RNA polymerase II) of isolates recovered from these vineyards indicated that Eutypa dieback is caused primarily by an undescribed Eutypa sp. (usda.gov)
- Comparisons of isolates of Heterodera avenae using 2-D PAGE protein patterns and ribosomal DNA. (plantprotection.pl)
- We investigated genetic variation among 52 morphologically and geographically diverse, indoor and outdoor isolates of S. chartarum sensu lato using molecular markers based on β-tubulin, calmodulin, elongation factor-1 alpha, and trichodiene synthase genes, as well as the internal transcribed spacer region of nuclear ribosomal DNA. (sporometrics.com)
- The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. (bvsalud.org)
Fungi2
- Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. (illinois.edu)
- Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. (illinois.edu)
Genes1
- in Europe (Finland, Norway and Switzerland) and differs from D. parca according to a five-locus alignment consisting of ITS, LSU, partial 18S nuclear ribosomal RNA, translation elongation factor 1- alpha and beta-tubulin genes. (up.ac.za)
Variation2
- Variation in spacer ribosomal DNA in some cyst- forming species of plant parasitic nematodes. (plantprotection.pl)
- This report presents results from a systematic screen for variation in repetitive DNA in the genome of C. difficile . (biomedcentral.com)
Region1
- The internal transcribed spacer (ITS) region of nuclear ribosomal DNA is the most frequently used marker for distinguishing plant species. (academicjournals.org)
Genome5
- It is hypothesized that genome size in polyploids tends to a maximum as it follows saturation behaviour, in agreement with the Michaelis-Menten model, and is suggested a theoretical upper limit for the DNA content of this genus. (semanticscholar.org)
- DNA base composition and genome size of the prokaryotic symbiont in Riftia pachyptila (Pogonophora). (whoi.edu)
- Single molecule, real-time (SMRT) sequencing was used to characterize mitochondrial (mt) genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. (frontiersin.org)
- In addition, the mt genome-wide base modification pattern of O. sinensis was determined in this study, especially DNA methylation. (frontiersin.org)
- We conclude that the targets of both DNA methylation and histone H3K9 methylation pathways are transposable elements genome-wide, irrespective of element type and position. (biomedcentral.com)
Species2
- Internal Transcribed Spacer (ITS) 1 and 2 were sequenced for five species of the genus Phyllanthus namely P. emblica , P. reticulatus , P. amarus , P. fraternus and P. urinaria , with a view to identify accurately herbaceous species. (scialert.net)
- Non-coding satellite DNA (satDNA) usually has a high turn-over rate frequently leading to species specific patterns. (biomedcentral.com)
Markers2
- The results confirm that methodological problems posed by RAPD markers can be avoided by careful laboratory procedures and appropriate data analyses and suggest that this kind of marker is useful at low taxonomic levels and is, furthermore, complementary to DNA sequence analysis. (semanticscholar.org)
- RAPD (Random Amplified Polymorphic DNA) markers have been most widely used for this purpose 8-10 . (bvsalud.org)
Genomic1
- Quality of genomic DNA was verifi ed growing on plant debris. (cdc.gov)
Bartonella DNA2
Methylation14
- A systematic assessment of cell type deconvolution algorithms for DNA methylation data. (cdc.gov)
- Global DNA methylation of WTC prostate cancer tissues show signature differences compared to non-exposed cases. (cdc.gov)
- Global DNA methylation profiles in peripheral blood of WTC-exposed community members with breast cancer. (cdc.gov)
- DNA methylation occurs at preferred sites in eukaryotes. (biomedcentral.com)
- In Arabidopsis , DNA cytosine methylation is maintained by three subfamilies of methyltransferases with distinct substrate specificities and different modes of action. (biomedcentral.com)
- Our findings also suggest that RNA-directed DNA methylation is required to silence isolated elements that may be too small to be maintained in a silent state by a chromatin-based mechanism alone. (biomedcentral.com)
- DNA cytosine methylation is an ancient process, found in both prokaryotes and eukaryotes, and catalyzed by a single family of methyltransferases [ 1 ]. (biomedcentral.com)
- In prokaryotes, cytosine-5 methyltransferases protect target sites from cleavage by partner restriction endonucleases, but in eukaryotes, the function of DNA methylation is less clear. (biomedcentral.com)
- An additional form of DNA methylation is found in the model plant Arabidopsis , where short dense CG methylation clusters are occasionally found in genic regions that are otherwise devoid of methylation [ 8 ]. (biomedcentral.com)
- Although many DNA methylation targets are known, it has been unclear how these sites are recognized by DNA methyltransferases. (biomedcentral.com)
- however, the mechanism by which siRNA production leads to de novo DNA methylation is not known. (biomedcentral.com)
- Another open question is how some forms of DNA methylation are maintained during rounds of cell division. (biomedcentral.com)
- In the case of CG sites, a member of the Dnmt1 subfamily of DNA methyltransferases maintains methylation by specifically methylating hemi-methylated sites behind the replication fork [ 15 ], but in cases of non-CG methylation, there does not appear to be a comparable reaction. (biomedcentral.com)
- Non-CG methylation in Neurospora is maintained by the action of a histone H3 lysine-9 (H3K9) methyltransferase [ 5 ], so the successive action of a histone methyltransferase and a DNA methyltransferase suffices to maintain methylation indefinitely. (biomedcentral.com)
Regions3
- 4 ) the internal transcribed spacer regions and the partial cell and Yëgues-Rodriguez et al. (cdc.gov)
- In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 11 populations. (plantprotection.pl)
- By analyzing nucleotide polymorphism of the chloroplast, we propose 21 hypervariable regions as potential DNA barcode regions for Myrtales. (biomedcentral.com)
Molecular1
- It was found that internal transcribed spacer 1 showed more potential than internal transcribed spacer 2 in assessing their relationship at molecular level. (scialert.net)
Nematodes1
- Here, we further explored the grouping effectiveness of the CV method in large DNA barcode datasets (COI, 18S and 16S rRNA) from a variety of organisms, including birds, fishes, nematodes and crustaceans. (biomedcentral.com)
Mitochondrial1
- Scatter plots and statistical tests revealed a significant correlation between genetic distances for satDNA and mitochondrial DNA. (biomedcentral.com)
Commonly1
- We have explored the effect of DSBs on targeted integration in bloodstream-form Trypanosoma brucei, focusing on the ribosomal RNA-spacer target commonly used to integrate recombinant constructs. (pasteur.fr)
Large1
- We conclude that the CV method is a fast and reliable method for analyzing large datasets for DNA barcoding purposes. (biomedcentral.com)
Eukaryotes1
- Discrepancies between prokaryotes and eukaryotes need to be considered in soil DNA-based studies. (myxotropic.org)
Target1
- We used a microarray-based profiling method to explore the involvement of Arabidopsis CMT3 and DRM DNA methyltransferases, a histone H3 lysine-9 methyltransferase (KYP) and an Argonaute-related siRNA silencing component (AGO4) in methylating target loci. (biomedcentral.com)
Study1
- A flow cytometric study of nuclear DNA content in a wide range of taxa of the Asteraceae family is carried out, filling gaps in some less studied groups. (semanticscholar.org)
Plant1
- 3α-Angeloyloxy-ent-kaur-16-en-19-oic acid (WT-25) is an ent-kaurane dieterpenoid extracted from Wedelia trilobata, a medicinal plant with potential anti-inflammatory activity. (bvsalud.org)