DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).
Any of the DNA in between gene-coding DNA, including untranslated regions, 5' and 3' flanking regions, INTRONS, non-functional pseudogenes, and non-functional repetitive sequences. This DNA may or may not encode regulatory functions.
Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.
Constituent of the 60S subunit of eukaryotic ribosomes. 5.8S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The relationships of groups of organisms as reflected by their genetic makeup.
Devices that cause a liquid or solid to be converted into an aerosol (spray) or a vapor. It is used in drug administration by inhalation, humidification of ambient air, and in certain analytical instruments.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
A small aerosol canister used to release a calibrated amount of medication for inhalation.
Repetitive nucleic acid sequences that are principal components of the archaeal and bacterial CRISPR-CAS SYSTEMS, which function as adaptive antiviral defense systems.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Procedures for identifying types and strains of fungi.
Infections resulting from the implantation of prosthetic devices. The infections may be acquired from intraoperative contamination (early) or hematogenously acquired from other sites (late).
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.
A short-acting beta-2 adrenergic agonist that is primarily used as a bronchodilator agent to treat ASTHMA. Albuterol is prepared as a racemic mixture of R(-) and S(+) stereoisomers. The stereospecific preparation of R(-) isomer of albuterol is referred to as levalbuterol.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
A series of hydrocarbons containing both chlorine and fluorine. These have been used as refrigerants, blowing agents, cleaning fluids, solvents, and as fire extinguishing agents. They have been shown to cause stratospheric ozone depletion and have been banned for many uses.
Adhesives used to fix prosthetic devices to bones and to cement bone to bone in difficult fractures. Synthetic resins are commonly used as cements. A mixture of monocalcium phosphate, monohydrate, alpha-tricalcium phosphate, and calcium carbonate with a sodium phosphate solution is also a useful bone paste.
The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.
Agents that cause an increase in the expansion of a bronchus or bronchial tubes.
Constituent of the 60S subunit of eukaryotic ribosomes. 28S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Genotypic differences observed among individuals in a population.
Copies of nucleic acid sequence that are arranged in opposing orientation. They may lie adjacent to each other (tandem) or be separated by some sequence that is not part of the repeat (hyphenated). They may be true palindromic repeats, i.e. read the same backwards as forward, or complementary which reads as the base complement in the opposite orientation. Complementary inverted repeats have the potential to form hairpin loop or stem-loop structures which results in cruciform structures (such as CRUCIFORM DNA) when the complementary inverted repeats occur in double stranded regions.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Compressed gases or vapors in a container which, upon release of pressure and expansion through a valve, carry another substance from the container. They are used for cosmetics, household cleaners, and so on. Examples are BUTANES; CARBON DIOXIDE; FLUOROCARBONS; NITROGEN; and PROPANE. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Genetic loci which direct transcription of ribosomal RNA in bacterial operons. They are designated rrnB, rrnC, rrnD, etc. according to the structural position of the transcription unit in the DNA sequence.
A transfer RNA which is specific for carrying alanine to sites on the ribosomes in preparation for protein synthesis.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
Deoxyribonucleic acid that makes up the genetic material of CHLOROPLASTS.
Constituent of the 50S subunit of prokaryotic ribosomes containing about 120 nucleotides and 34 proteins. It is also a constituent of the 60S subunit of eukaryotic ribosomes. 5S rRNA is involved in initiation of polypeptide synthesis.
Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
An anti-inflammatory, synthetic glucocorticoid. It is used topically as an anti-inflammatory agent and in aerosol form for the treatment of ASTHMA.
A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.
Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".
A plant family of the order Magnoliales, subclass Magnoliidae, class Magnoliopsida. The wood lacks water-conducting cells but has acrid sap. The leaves are gland-dotted, leathery, and smooth-margined. The flowers are small, in clusters, with two to six sepals, petals in two or more series, several stamens, and one to several carpels.
The profession of writing. Also the identity of the writer as the creator of a literary production.
An island in the Greater Antilles in the West Indies, south of Florida. With the adjacent islands it forms the Republic of Cuba. Its capital is Havana. It was discovered by Columbus on his first voyage in 1492 and conquered by Spain in 1511. It has a varied history under Spain, Great Britain, and the United States but has been independent since 1902. The name Cuba is said to be an Indian name of unknown origin but the language that gave the name is extinct, so the etymology is a conjecture. (From Webster's New Geographical Dictionary, 1988, p302 & Room, Brewer's Dictionary of Names, 1992, p132)
A plant genus in the family PINACEAE, order Pinales, class Pinopsida, division Coniferophyta. They are coniferous evergreen trees with long, flat, spirally arranged needles that grow directly from the branch.

Utility of internally transcribed 16S-23S rDNA spacer regions for the definition of Pseudomonas stutzeri genomovars and other Pseudomonas species. (1/1629)

Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA-DNA similarities. The internally transcribed 16S-23S rDNA spacer (ITS1) regions of P. stutzeri were analysed with respect to the ability of these nucleic acid regions to differentiate and identify the genomic groups (i.e. genomovars) of P. stutzeri. The ITS1s of 34 strains of P. stutzeri were amplified by PCR and the PCR product was subjected to RFLP analysis, which allowed the differentiation and identification of the strains to their respective genomovars. Sequence determination and analysis of ITS1s supported further the results obtained by RFLP, i.e. nucleotide signatures were identified in strains belonging to different genomovars. The ITS1s of all strains of P. stutzeri contained the tandem tRNA(Ile)/tRNA(Ala) genes and did not exhibit distinct sequence heterogeneity between different operons of a strain. Phylogenetically informative variable sites were located, exclusively, in non-coding regions. The results of the RFLP and sequence analysis of ITS1s supported and correlated with the phylogenetic relationships estimated from 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, offering an alternative tool for genomovar and species differentiation.  (+info)

High intraindividual variation in internal transcibed spacer sequences in Aeschynanthus (Gesneriaceae): implications for phylogenetics. (2/1629)

Aeschynanthus (Gesneriaceae) is a large genus of tropical epiphytes that is widely distributed from the Himalayas and China throughout South-East Asia to New Guinea and the Solomon Islands. Polymerase chain reaction (PCR) consensus sequences of the internal transcribed spacers (ITS) of Aeschynanthus nuclear ribosomal DNA showed sequence polymorphism that was difficult to interpret. Cloning individual sequences from the PCR product generated a phylogenetic tree of 23 Aeschynanthus species (two clones per species). The intraindividual clone pairs varied from 0 to 5.01%. We suggest that the high intraindividual sequence variation results from low molecular drive in the ITS of Aeschynanthus. However, this study shows that, despite the variation found within some individuals, it is still possible to use these data to reconstruct phylogenetic relationships of the species, suggesting that clone variation, although persistent, does not pre-date the divergence of Aeschynanthus species. The Aeschynanthus analysis revealed two major clades with different but overlapping geographic distributions and reflected classification based on morphology (particularly seed hair type).  (+info)

High-resolution phylogenetic analysis of NO2--oxidizing Nitrobacter species using the rrs-rrl IGS sequence and rrl genes. (3/1629)

A high-resolution phylogenetic analysis of Nitrobacter strains and their neighbours was made using the rrs-rrl intergenic spacer sequence and the hypervariable part of the rrl gene. The phylogenetic tree obtained was consistent with that which was obtained previously but was much more discriminating, permitting the design of genus-specific primers.  (+info)

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae. (4/1629)

On the basis of genetic analysis, molecular karyotyping and sequence analyses of the 18S rRNA and internal transcribed spacer (ITS) region, three new Saccharomyces species are described, Saccharomyces cariocanus (with type strain NCYC 2890T), Saccharomyces kudriavzevii (with type strain NCYC 2889T) and Saccharomyces mikatae (with type strain NCYC 2888T). Genetic and molecular analyses did not confirm the previously observed conspecificity of Saccharomyces paradoxus and S. cariocanus. The latter species exhibits postzygotic isolation from representative strains from all known geographical populations of S. paradoxus: European, Far-East Asian, North American and Hawaiian.  (+info)

Molecular systematics of European Hyalodaphnia: the role of contemporary hybridization in ancient species. (5/1629)

We examined phylogenetic relationships among Daphnia using mitochondrial DNA (mtDNA) sequences from the small subunit ribosomal RNA (12S), cytochrome c oxidase subunit I and nuclear DNA sequences from the first and second internal transcribed spacer representing 1612 base positions. Phylogenetic analyses using several species of the three main Daphnia subgenera, Ctenodaphnia, Hyalodaphnia and Daphnia, revealed that the Hyalodaphnia are a monophyletic sister group of the Daphnia. Most Hyalodaphnia species occur on one continent, whereas only three are found in North America and Europe. Endemicity of species is associated with variation in thermal tolerance and habitat differentiation. Although many species of the Hyalodaphnia are known to hybridize in nature, mtDNA divergence is relatively high ca. 9%) compared to other hybridizing arthropods (ca. 3%). Reproductive isolation in Daphnia seems to evolve significantly slower than genetic isolation. We related these findings to what is known about the ecology and genetics of Daphnia in order to better understand the evolutionary diversification of lineages. The relationship of these data to phylogenetic patterns is discussed in the context of speciation processes in Daphnia.  (+info)

Detection and identification of mycobacteria by amplification of the internal transcribed spacer regions with genus- and species-specific PCR primers. (6/1629)

We evaluated the usefulness of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species level. The conservative and species-specific ITS sequences of 33 species of mycobacteria were analyzed in a multialignment analysis. One pair of panmycobacterial primers and seven pairs of mycobacterial species-specific primers were designed. All PCRs were performed under the same conditions. The specificities of the primers were tested with type strains of 20 mycobacterial species from the American Type Culture Collection; 205 clinical isolates of mycobacteria, including 118 Mycobacterium tuberculosis isolates and 87 isolates of nontuberculous mycobacteria from 10 species; and 76 clinical isolates of 28 nonmycobacterial pathogenic bacterial species. PCR with the panmycobacterial primers amplified fragments of approximately 270 to 400 bp in all mycobacteria. PCR with the M. tuberculosis complex-specific primers amplified an approximately 120-bp fragment only for the M. tuberculosis complex. Multiplex PCR with the panmycobacterial primers and the M. tuberculosis complex-specific primers amplified two fragments that were specific for all mycobacteria and the M. tuberculosis complex, respectively. PCR with M. avium complex-, M. fortuitum-, M. chelonae-, M. gordonae-, M. scrofulaceum-, and M. szulgai-specific primers amplified specific fragments only for the respective target organisms. These novel primers can be used to detect and identify mycobacteria simultaneously under the same PCR conditions. Furthermore, this protocol facilitates early and accurate diagnosis of mycobacteriosis.  (+info)

Divergent mechanisms of 5' 23S rRNA IVS processing in the alpha-proteobacteria. (7/1629)

Widespread occurrence of a separate small RNA derived from the 5'-end of 23S rRNA and of an intervening sequence (IVS) which separates this domain from the main segment of 23S rRNA in the alpha-proteobacteria implies that processing reactions which act to excise the IVS are also maintained in this group. We previously characterized the first example of processing of this IVS in Rhodopseudomonas palustris, which is classified with the Bradyrhizobia In this case, IVS excision occurs by a multistep process and RNase III appears to act at an early step. Here, we characterize in vivo and in vitro IVS processing in two other related, but phenotypically distinct, Bradyrhizobia We also examine in vivo and in vitro processing of rRNA precursors from a more distantly related alpha-proteobacterium, Rhodobacter sphaeroides which produces a separate 5' 23S rRNA domain but has different sequences in the 5' 23S rRNA IVS. The details of the in vivo processing of all of the Bradyrhizobial rRNAs closely resemble the R. palustris example and in vitro studies suggest that all of the Bradyrhizobia utilize RNase III in the first step of IVS cleavage. Remarkably, in vivo and in vitro studies with R.sphaeroides indicate that initial IVS cleavage uses a different mechanism. While the mechanism of IVS cleavage differs among these alpha-proteobacteria, in all of these cases the limits of the internal segments processed in vivo are almost identical and occur far beyond the initial cleavage sites within the IVSs. We propose that these bacteria possess common secondary maturation pathways which enable them to generate similarly processed 23S rRNA 5'- and 3'-ends.  (+info)

Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. (8/1629)

Trichophyton rubrum is the commonest cause of dermatophytosis of skin and nail tissue. Molecular characterization of the T. rubrum ribosomal DNA nontranscribed-spacer region revealed two novel tandemly repetitive subelements (TRSs): TRS-1, containing a 27-bp palindromic sequence, and TRS-2. Specific amplification of TRS-1 produced strain-characteristic banding patterns (PCR types), with 21 TRS-1 PCR types recognized from 101 clinical isolates. Four simple patterns representing 1 to 4 copies of TRS-1 accounted for 75 (75%) of all 101 strains, whereas more complex patterns were observed for 21 (20%) of the 101 isolates. The copy number of TRS-2 was 0 to 3 repeats per cistron, with a majority of isolates having two copies of this element. Eleven isolates were polymorphic for TRS-2, and in combination, 23 separate PCR types were recognized by amplification of both TRS-1 and TRS-2. The PCR patterns from both elements were stable and reproducible. Elements with homology to TRS-1 were present in three phylogenetically related species, Trichophyton violaceum, Trichophyton gourvilii, and Trichophyton soudanense, but these elements were not identified in other dermatophyte taxa. There was no clear correlation of PCR type with specimen (skin or nail tissue), but certain PCR types appeared to show a bias in geographic distribution. This new method of typing T. rubrum will enable important questions about pathogenesis and epidemiology of this fungus to be addressed.  (+info)

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Chen, H.,Lim, C.K.,Lee, Y.K.,Chan, Y.N. (2000). Comparative analysis of the genes encoding 23S-5S rRNA intergenic spacer regions of Lactobacillus casei-related strains. International Journal of Systematic and Evolutionary Microbiology 50 (2) : 471-478. [email protected] Repository ...
The 2,030 isolates analyzed in the present study comprised 1,235 isolates from stool samples from hospital patients, 395 isolates from stool samples referred via community practitioners, 150 isolates from the hospital environment, 27 isolates from veterinary sources, 89 isolates from the general environment, 1 isolate from an extraintestinal human site, and 133 reference strains held in the National Collection of Type Cultures, the American Type Culture Collection and the Culture Collection, University of Göteborg, and in the personal collections of C. difficile types held by Delmee and others (6, 16) and other members of the International Study Group on C. difficile (3).. PCR ribotyping was performed in duplicate, with slight modifications to a method described previously (13). Briefly, bacteria were harvested from overnight anaerobic cultures on Fastidious Anaerobe Agar (LabM, Bury, United Kingdom) supplemented with 6% horse blood. Crude template nucleic acid was prepared by resuspension of ...
Staphylococcus aureus is an important human and animal pathogen, and is regarded as an important cause of intramammary infection (IMI) in ruminants. Staphylococcus aureus genetic variability and virulence factors have been well studied in veterinary medicine, especially in cows as support for control and management of IMI. The aim of the present study was to genotype 71 Staph. aureus isolates from the bulk tank and foremilk of water buffaloes (n. = 40) and from udder tissue (n. = 7) and foremilk (n. = 24) from small ruminants. The method used was previously applied to bovine Staph. aureus and is based on the amplification of the 16S-23S rRNA intergenic spacer region. The technique applied was able to identify different Staph. aureus genotypes isolated from dairy species other than the bovine species, and cluster the genotypes according to species and herds. Virulence gene distribution was consistent with genotype differentiation. The isolates were also characterized through determination of the ...
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The University of Auckland Library This study describes the extent of variability in biofilm bacterial community structure across a broad range of spatial and temporal scales and assesses whether this may be used as an indicator of stream ecological health and function. A community DNA fingerprinting technique (Automated Ribosomal Intergenic Spacer Analysis - ARISA) was used to examine the structure of bacterial communities within freshwater stream biofilms. When compared with macrobenthic invertebrate community assemblages using multi-dimensional scaling techniques, similar broad-scale trends in population structure were revealed between organisms at these different trophic levels. For both communities, spatial variability in community structure was greater between streams than within each site, or compared to temporal variability measured over 1 year. Distance-based redundancy analysis of both bacterial ARISA and macroinvertebrate data estimated that the largest cause of variation in community ...
Freshwater streams display both temporal (time) and spatial (space) differences. Time variation is a result of seasonal influences and space variation is due to flow regime, substrate type, water solutes, suspended materials and incident light exposure. It is believed that bacterial communities are good indicators due to their rapid life cycle. However, if we cant see them, how do we know that they are present and observe changes? Bacteria can be detected using Automated Ribosomal Intergenic Spacer Analysis (ARISA) which creates fingerprints of microbial communities. The fingerprint produced is just like a human fingerprint, because it is unique to a bacteria species just like a fingerprint is unique to a person ...
The Crystal Refinery is a former refinery site in Carson City Michigan that has had historic releases of refined and unrefined crude oil dating back to the late 1940s. The contamination of soil with light non-aqueous phase liquids (LNAPL) has had a dynamic impact on the subsurface environment in adjacent Carson City Park. Very little is known about the complex interactions between microbial communities, geochemistry and geophysics. In order to investigate possible connections between these parameters a multidisciplinary study was undertaken to investigate the hypothesis that the degradation of LNAPL by resident microbial communities causes a local increase in organic acid concentrations, which in turn cause an increase in native mineral weathering and a concurrent decrease in the bulk electrical resistivity of soil.Microbial community structure was analyzed using a 96-well most probable number (MPN) method and rDNA intergenic spacer region analysis (RISA). In addition to the observational study of this
An apparatus for killing insects in produce, which comprises an outer container; means for dividing a space enclosed by the outer container into first and second internal chambers; means for supporting a plurality of bulk produce bins in the first internal chamber; first and second air passage means for selectively allowing passage of air between the first internal chamber and the second internal chamber; means for circulating air in the second internal chamber successively through one of the air passage means, the first internal chamber, the other of the air passage means, and back into the second internal chamber; and means for heating the circulating air.
A trial spacer kit has a plurality of trial spacers of varying heights. Each spacer includes a disc having a top surface and a bottom surface defining a height of said spacer, an annular groove formed in said disc that defines a central trunk of said spacer, the central trunk extending between the top and bottom surfaces of said spacer, a first radial flange provided at an upper end of the central trunk and a second radial flange provided at a lower end of the central trunk. The annular grooves of the spacers have a common dimension. The radial flanges of a first one of the spacers is thicker than the radial flanges of a second one of the spacers so that the first one of the spacers has a greater height than the second one of the spacers.
Under the intluence of 5-tluoro-uridine, the ultrastructure of the rDNA transcription units in Xenopus oocytes is altered. Whereas part of the matrix units maintains anormal aspect or shows various degrees of inhibition, in a strong proportion of the transcription units the alternating pattern of matrix units and fibril-free spacer regions is no longer recognized. Transcriptional complexes are found along the entire DNP axis, including the regions of the spacers. These observations support biochemical data on transcription in rDNA spacer region ...
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Lactobacillus acetotolerans strain ATCC 43578 16S ribosomal RNA gene,partial sequence; 16S-23S intergenic spacer, complete sequence; and 23Sribosomal RNA gene, partial ...
關於生長在藻類上的真菌研究缺乏,特別是海洋大型藻類,而前人只針對小部份褐藻、綠藻和紅藻進行初部研究。因此本研究選取臺灣北部的優勢紅藻細翼枝菜 (Pterocladiella capillacea) 作為研究對象,以培養法分離真菌及非培養法 (metabarcoding) 結果比較細翼枝菜上的真菌多樣性。於培養法中,將洗淨的健康及死亡藻體置於葡萄糖-酵母萃取物-蛋白腖瓊脂海水培養基 (GYPS) 及人工海水瓊脂培養基 (SWA) 上,待菌絲從藻體生長出來後便繼代於玉米粉瓊脂培養基 (CMAS) 上,依其菌落形態分群。利用聚合酶連鎖反應 (polymerase chain reaction, PCR) 擴增其核醣體基因 (rDNA) 內轉錄間隔區 (internal transcribed spacer, ITS) 及核醣體大亞基 (large subunit, LSU) 並進行測序,最後與NCBI的GenBank資料庫序列進行比較以鑑種。於高通量定序中,從冷凍乾燥後的細翼枝菜藻體萃取總DNA和RNA
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Ribosomal DNA (rDNA) of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer (IGS). The 45S rDNA of flowering plants includes the 5.8S, 18S and 25S rDNA genes, the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). This study identified six different types of 45S-IGS, A to F, which at 363 bp, 1121 bp, 1717 bp, 1969 bp, 2036 bp and 2111 bp in length, respectively, were much shorter than the reported reference IGS sequences in B. oleracea var. alboglabra. The shortest two IGS types, A and B, lacked the transcription initiation site, non-transcribed spacer, and external transcribed spacer. Functional behavior of those two IGS types in relation to rRNA synthesis is a subject of further investigation. The other four IGSs had subtle variations in the transcription termination site, guanine-cytosine (GC) content, and number of tandem repeats, but the external transcribed spacers of these four IGSs were
While the 16S ribosomal gene is the standard for distinguishing among bacterial species, it is insufficiently variable among P. gingivalis strains for resolution. The ribosomal ISR is the most variable portion of the ribosomal operon and is sufficiently variable within a species to resolve strains (18). This locus also offers a technical advantage since it can be amplified with species-specific and conserved primers in the 16S and 23S ribosomal genes, thus avoiding the need to culture bacteria from clinical specimens. There are four copies of the ribosomal operon in P. gingivalis (P. gingivalis genome project [http://www.forsyth.org/pggp/number_of_rrna_operons.htm]). Ribosomal operons in general, including the ISR, are maintained by concerted evolution (2, 4). This correction minimizes replacement by exogenous sequences, so that the operon should closely reflect the evolutionary history of the organism.. We have previously used the ISR for both sequence-based and heteroduplex type-based P. ...
Author contributions: C.L.S. and K.A.S. designed research; K.A.S., V.R., E.B., K.V., P.W.C., A.N.M., M.J.W., M.C.A., K.-D.A., F.-Y.B., R.W.B., D.B., M.-J.B., M. Blackwell, T.B., M. Bogale, N.B., A.R.B., B.B., L.C., Q.C., G.C., P. Chaverri, B.J.C., A.C., P. Cubas, C.C., U.D., Z.W.d.B., G.S.d.H., R.D.-P., B. Dentinger, J.D-U., P.K.D., B. Douglas, M.D., T.A.D., U.E., J.E.E., M.S.E., K.F., M.F., M.A.G., Z.-W.G., G.W.G., K.G., J.Z.G., M. Groenewald, M. Grube, M. Gryzenhout, L.-D.G., F. Hagen, S. Hambleton, R.C.H., K. Hansen, P.H., G.H., C.H., K. Hirayama, Y.H., H.-M.H., K. Hoffmann, V. Hofstetter, F. Högnabba, P.M.H., S.-B.H., K. Hosaka, J.H., K. Hughes, Huhtinen, K.D.H., T.J., E.M.J., J.E.J., P.R.J., E.B.G.J., L.J.K., P.M.K., D.G.K., U.K., G.M.K., C.P.K., S.L., S.D.L., A.S.L., K.L., L.L., J.J.L., H.T.L., H.M., S.S.N.M., M.P.M., T.W.M., A.R.M., A.S.M., W.M., J.-M.M., S.M., L.G.N., R.H.N., T.N., I.N., G.O., I. Okane, I. Olariaga, J.O., T. Papp, D.P., T. Petkovits, R.P.-B., W.Q., H.A.R., D.R., T.L.R., ...
Respiratory infection in cystic fibrosis (CF) is polymicrobial, but standard sputum microbiology does not account for the lung microbiome or detect changes in microbial diversity associated with disease. As a clinically applicable CF microbiome surveillance scheme, total sputum nucleic acids isolated by a standard high-throughput robotic method for accredited viral diagnosis were profiled for bacterial diversity using ribosomal intergenic spacer analysis (RISA) PCR. Conventional culture and RISA were performed on 200 paired sputum samples from 93 CF adults; pyrosequencing of the 16S rRNA gene was applied to 59 patients to systematically determine bacterial diversity. Compared to the microbiology data, RISA profiles clustered into two groups: the emerging nonfermenting Gram-negative organisms (eNFGN) and Pseudomonas groups. Patients who were culture positive for Burkholderia, Achromobacter, Stenotrophomonas, and Ralstonia clustered within the eNFGN group. Pseudomonas group RISA profiles were ...
In recent years, DNA barcoding is considered as a universal species identification method for plants. It mainly involves discrimination of species through standardized molecular marker gene and is gaining support from the taxonomists as well. DNA barcoding has wider applications in different studies namely to predict cryptic species, to study biological samples in forensics and conservation sciences for characterization of biodiversity; to track inventory for plants identity or purity and in ecological species diversity studies [1-4]. Various molecular markers have been used for DNA barcoding studies. A 650 base pair (bp) of the mitochondrial cytochrome c oxidase unit I (COI) gene was used as a barcode in various organisms such as animals, birds, fishes, insects and nematodes [4-8]. A specific region of the nuclear ribosomal internal transcribed spacer (ITS) gene is the well-studied DNA barcode for fungi [9]. In plant DNA barcoding, there has been extensive debate about the locus choice; several ...
TY - JOUR. T1 - Report of the second internal workshop on human chromosome 5 mapping. AU - Westbrook, C. A.. AU - Neuman, W. L.. AU - Mcpherson, John Douglas. AU - Camper, S.. AU - Wasmuth, J.. AU - Plaetke, R.. AU - Williamson, R.. PY - 1992. Y1 - 1992. UR - http://www.scopus.com/inward/record.url?scp=0026677059&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026677059&partnerID=8YFLogxK. M3 - Article. C2 - 1362532. AN - SCOPUS:0026677059. VL - 61. SP - 226. EP - 231. JO - Cytogenetic and Genome Research. JF - Cytogenetic and Genome Research. SN - 1424-8581. IS - 4. ER - ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
the spacers around your I am guessing if your son has perfect spacers for your Using your Spacer Eibach PRO-SPACER, FERRARI 360 teeth enhance your teeth side cone spacer and your SPACERS FOR TEETH - Page 5 what are spacers for your Keep his teeth andental health spacers for teeth, google Spacers For Your Teeth yahoo Spacers For Your Teeth mages images ...
Spacer 9 is a triethylene glycol chain that is 9 atoms long (6 carbons + 3 oxygens), and is used to incorporate a spacer arm into an oligonucleotide. Spacer 9 can be incorporated in consecutive additions whenever a longer spacer is required. Spacer 9 has been used to form non-nucleotide bridges in hairpin loops in oligonucleotides (1), for linking oligonucleotides to epitopes for drug development (2), and for solid-phase immobilization of hybridization probes (3). Multiple incorporation of Spacer 9 has been used to form long, flexible linker arms between the two domains (double-helix forming and triple-helix forming, respectively) of a bifunctional DNA oligonucleotide, in order to maximize the binding flexibility of the two domains for their respective targets (4). This oligo was used to form a peptide nucleic acid (PNA)-DNA conjugate for use in site-directed recombination applications.. References ...
We explored the potential of the cox1 gene in the species resolution of soil fungi and compared it with the nuclear internal transcribed spacer (ITS) and small subunit (SSU)-rDNA. Conserved primers allowing the amplification of the fungal cox1 gene were designed, and a total of 47 isolates of Zygomycota and Ascomycota were investigated. The analysis revealed a lack of introns in >90% of the isolat ...
Item Description Combs Used between the 62cm glass plates of the JVD-80 Must be matched with a 3.0mm thick comb Includes two side spacers, one bottom spacer, and two spacer tabs The SP-3062 Spacer Set is designed for pouring 62cm long polyacrylamide gels of 3.0mm thickness. This spacer set contains three white, polysty
Lactobacillus kefiranofaciens strain ATCC 43761 16S ribosomal RNA gene,partial sequence; 16S-23S intergenic spacer, complete sequence; and 23Sribosomal RNA gene, partial ...
This page features a historical overview and special image selection on the Guadalcanal Campaign of August 1942 to February 1943.
Overview This course will introduce students to the field of biosystematics and will include history, theory, and practice of both taxonomy (the original bioinformatics) and modern phylogenetics. The methods covered apply equally to all eukaryotic taxa, based on either morphological or molecular characters. Training in key software packages will take place on Fridays. Some of the topics and methods covered:. ...
The spacers added to the Verifix® MV 760 adhesive guarantee that the required minimum adhesive layer thickness is reached. This makes sure that, when bonding very heavy glass parts, sufficient adhesive is distributed between the bonding parts. Depending on the bonding process, this method has also proven useful when bonding metal in order to maintain the minimum adhesive layer thickness. The spacers do not interfere with the technical properties of the adhesive. Please read the supplied processing guidelines carefully. If you have any questions, our application technicians will be happy to assist you.. ...
I recently added these wheel spacers from Just Differentials: 1.25 Wheel Spacers Unfortunately I immediately started experiencing significant vibration...
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The internal transcribed spacer 2 database--a web server for (not only) low level phylogenetic analyses. Schultz, J.; Muller, T.; Achtziger, M.; Seibel, P. N.; Dandekar, T.; Wolf, M. in Nucleic Acids Res. (2006). 34 W704--707. ...
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Highly retentive and selective for protonated basic analytes and molecules containing aromatic moities. End-capped pentafluorophenyl with a propyl spacer.
Purchased this fork through CRC. Can someone explain to me why some of these units are not as advertised. Mine stated 80mm or 120mm through spacers.
Two xylose-utilizing yeast strains isolated from rotten wood collected in the rainforest in different mountains of Hainan province, southern China, were studied. Sequence analysis of the large subunit rDNA D1/D2 domain and internal transcribed spacer region revealed that the strains represent a novel anamorphic yeast species, for which the name Candida cellulosicola sp. nov. is proposed; the type strain is HNX16-2T ( = CGMCC 2.3503T = CBS 11952T). Phylogenetically, the novel species was closely related to a xylose-utilizing teleomorphic ascomycetous yeast species Spencermartinsiella europaea in the family Trichomonascaceae, but differed from the latter by 3.0 % mismatches in the D1/D2 domain.
Systematics Section / ASPT. Wahlert, G. A. [1], Parker, V. Thomas [2], Vasey, Michael C. [2]. A Phylogeny of Arctostaphylos (Arbutoideae, Ericaceae) Inferred from nrITS Sequence Data.. A phylogeny of Arctostaphylos (Arbutoideae, Ericaceae) was estimated using the nuclear ribosomal Internal Transcribed Spacer (ITS) sequence data for 85 taxa and 16 accessions of widespread taxa. Forty-five taxa had identical ITS sequences and 17 taxa had polymorphic ITS nucleotide positions. Phylogenetic analyses resulted in two-clade topologies that were largely congruent with other studies in the genus, except that seven North American accessions of A. uva-ursi were consistently resolved as monophyletic. In four taxa, patterns of nucleotide additivity in the ITS region were suggestive of interspecific hybrid origin. Four chloroplast intergenic spacers (atpB-rbcL, trnL-trnF, trnG-trnS, and trnH-psbA) and the trnL intron were sequenced for nine geographically distant and morphologically divergent species. The ...
An rpoB sequence-based evaluation of 100 Mycobacterium avium complex (MAC) clinical isolates led to the identification of five respiratory tract isolates that were potential representatives of three novel MAC species. Distinctive phenotypic features of isolates 62863 and 5356591T included a pseudomycelium morphology and both esterase and acid phosphatase activities. These two isolates exhibited sequence similarities of 99.8 % for the 16S rRNA gene, 86.3 and 86.1 % for 16S-23S rRNA gene internal transcribed spacer (ITS-1) sequence, 96.7 and 97.8 % for rpoB and 97.6 and 97.4 % for hsp65, respectively, with the type strain of Mycobacterium chimaera, the most closely related species. Isolates 3256799 and 5351974T lacked α-mannosidase and β-glucosidase activities. They exhibited sequence similarities of 99.6 % for the 16S rRNA gene, 90.1 and 90.4 % for ITS-1, 97.8 % for rpoB and 98.0 and 98.1 % for hsp65, respectively, with the type strain of M. chimaera, the most closely related species. Isolate 4355387T
Dinoflagellates in the genus Symbiodinium are best known as endosymbionts of corals and other invertebrate as well as protist hosts, but also exist free-living in coastal environments. Despite their importance in marine ecosystems, less than 10 loci have been used to explore phylogenetic relationships in this group, and only the multi-copy nuclear ribosomal Internal Transcribed Spacer (ITS) regions 1 and 2 have been used to characterize fine-scale genetic diversity within the nine clades (A-I) that comprise the genus. Here, we describe a three-step molecular approach focused on 1) identifying new candidate genes for phylogenetic analysis of Symbiodinium spp., 2) characterizing the phylogenetic relationship of these candidate genes from DNA samples spanning eight Symbiodinium clades (A-H), and 3) conducting in-depth phylogenetic analyses of candidate genes displaying genetic divergences equal or higher than those within the ITS-2 of Symbiodinium clade C. To this end, we used bioinformatics tools ...
Analysis of the reference M. abscessus ATCC 19977 complete genome sequence yielded 3538 intergenic spacers with , 300 spacers were 200-700 bp in length. Successful PCR sequencing was achieved for 8 spacers in all the isolates studied; the sequences were deposited in the GenBank database (GenBank accession: KC352850 - KC352890). In M. abscessus isolates, including the 37 sequenced genomes, the spacer sequence variability was generated by one to 12 single nucleotide polymorphisms (SNPs) (spacers n°1 and n°8), one to 18 SNPs and one to two nucleotide deletions (spacer n°2), one to two SNPs (spacers n°3 and n°7) and nucleotide insertion (spacers n°2 and n°5). In M. bolletii isolates, the spacer sequence polymorphisms were generated by one SNP for spacer n°1, two SNPs and one deletion for spacer n°2, two SNPs for spacer n°3 and nine SNPs for spacer n°7. In M. massiliense isolates, including 28 sequenced genomes, the spacer sequence polymorphism were generated by nine SNPs and one ...
Nuclear ribosomal internal transcribed spacer (ITS) DNA sequences are used to estimate the phylogeny of 53 members of Polemoniaceae, representing all but two genera of the family. Fitch parsimony analysis of equal-weighted nucleotide sites result in 1080 minimal-length trees. However, when alignment-ambiguous positions are removed and an II: 10 transition to transversion weighting is imposed only eight trees are found. These data are used to address two issues: I) patterns of diversification in Polemoniaceae, and 2) the circumscription and monophyly of the genus Gilia. Although the monophyly of Polemoniaceae is well supported, relationships inferred among the earliest diverging lineages are altered by character weighting, treatment of indels, and taxon inclusion. In spite of the lack of reliable resolution at the basal nodes, ITS data provide evidence that Gilia, as currently interpreted, is polyphyletic and comprises at least five independent lineages.
Firdaus-e-Bareen & Uwe Braun. Mycofalcella iqbalii, a new aquatic hyphomycete, and a discussion about the generic concept of Anguillospora and similar genera. Mycotaxon 102: 355-363. 2007.. ABSTRACT: A new aquatic hyphomycete, Mycofalcella iqbalii, is described and illustrated from canal water in Pakistan. The conidia have been detected in water throughout the year, but sporulation on randomly collected leaves more commonly occurred between August and December. The generic affinity of the new species is debated in the context of a general discussion about the generic concepts of the complex of hyphomycetes resembling Anguillospora, Anguillosporella, Mycofalcella, and aquatic species of Mycocentrospora. It is proposed to confine Anguillospora to species with unilocal conidiogenous cells and rhexolytic conidial secession, and to widen the concept of Pseudoanguillospora to include taxa with unilocal, determinate or percurrent to multilocal, sympodial conidiogenous cells and schizolytic conidial ...
A phylogeny of the fungal phylum Basidiomycota. is presented based on a survey of 160 taxa and five nuclear genes. Two genes, rpb2, and tef1, are presented in detail. The rpb2 gene is more variable than tef1 and recovers well-supported clades at shallow and deep taxonomic levels. The tef1 gene recovers some deep and ordinal-level relationships but with greater branch support from nucleotides compared to amino acids. Intron placement is dynamic in tef1, often lineage-specific, and diagnostic for many clades. Introns are fewer in rpb2 and tend to be highly conserved by position. When both protein-coding loci are combined with sequences of nuclear ribosomal RNA genes, 18 inclusive clades of Basidiomycota are strongly supported by Bayesian posterior probabilities and 16 by parsimony bootstrapping. These numbers are greater than produced by single genes and combined ribosomal RNA gene regions. Combination of nrDNA with amino acid sequences, or exons with third codon positions removed, produces strong ...
Determination of phylogenetic relationships among ancestral species of Rubus has been elusive. Most Rubus species (including blackberries and raspberries), representing nine of the 12 subgenera, occur in a large, well supported clade named A for reference). The remaining nine species are excluded from this group and represent three subgenera: subg. Anoplobatus (R. bartonianus, R. deliciosus, R. neomexicanus, R. odoratus, R. parviflorus, R. trilobus), subg. Chamaemorus (R. chamaemorus), and subg. Dalibarda (R. lasiococcus, R.pedatus). In addition, Rubus dalibarda L. is often treated in its own monotypic genus as Dalibarda repens L. Phylogenetic analyses of DNA sequence data from chloroplast regions and the nuclear ribosomal DNA internal transcribed spacer ITS 1 - 5.8S - ITS 2; ITS) region have not resolved basal relationships in Rubus and the position of Dalibarda repens has varied from being the sister group to Rubus to nested within it. However, monophyly of American subg. Anoplobatus species is
This dataset contains the digitized treatments in Plazi based on the original journal article Straube, Nicolas, White, William T., Ho, Hsuan-Ching, Rochel, Elisabeth, Corrigan, Shannon, Li, Chenhong, Naylor, Gavin J. P. (2013): A DNA sequence-based identification checklist for Taiwanese chondrichthyans. Zootaxa 3752 (1): 256-278, DOI: http://dx.doi.org/10.11646/zootaxa.3752.1.16 ...
Handy reference books: 1) Molecular Systematics, 2nd ed. (Hillis, Moritz & Mable, eds. 1996, Sinauer) especially Chapter 11 by Swofford et al. on Phylogenetic Inference; 2) Molecular Evolution: A phylogenetic Approach (Page & Holmes 1998, Blackwell); 3) Inferring Phylogenies (Felsenstein 2004, Sinauer); The Phylogenetic Handbook (eds. Philippe Lemey, Marco Salemi, and Anne-Mieke Vandamme, 2010). Lecture Goals: The course will focus on the basics of molecular systematics theory and practice from the point of view of the data. We will explore the ways in which an understanding of processes of evolution of molecular data can help in the construction of evolutionary trees. Lectures will examine some of the most serious problems in evolutionary tree construction: nucleotide bias, alignment, homoplasy, among-site rate variation, taxon sampling, long branches, big trees, heterogeneous rates of evolution among branches, covarion shifts. Laboratory Goals: Labs will cover basic techniques in molecular ...
Handy reference books: 1) Molecular Systematics, 2nd ed. (Hillis, Moritz & Mable, eds. 1996, Sinauer) especially Chapter 11 by Swofford et al. on Phylogenetic Inference; 2) Molecular Evolution: A phylogenetic Approach (Page & Holmes 1998, Blackwell); 3) Inferring Phylogenies (Felsenstein 2004, Sinauer); The Phylogenetic Handbook (eds. Philippe Lemey, Marco Salemi, and Anne-Mieke Vandamme, 2010). Lecture Goals: The course will focus on the basics of molecular systematics theory and practice from the point of view of the data. We will explore the ways in which an understanding of processes of evolution of molecular data can help in the construction of evolutionary trees. Lectures will examine some of the most serious problems in evolutionary tree construction: nucleotide bias, alignment, homoplasy, among-site rate variation, taxon sampling, long branches, big trees, heterogeneous rates of evolution among branches, covarion shifts. Laboratory Goals: Labs will cover basic techniques in molecular ...
Recently collected material was used to evaluate the phylogenetic position of the secotioid fungus Araneosa columellata. We amplified the nuclear ribosomal RNA gene (ITS1, ITS2, and LSU) and found Ar. columellata to be a member of the genus Agaricus (Agaricaceae, Agaricales), necessitating its transfer to that genus as Agaricus columellatus comb. nov. A detailed description is provided along with illustrations, and a lectotype is designated ...
Major factor driving growth of the global asthma spacers market is increasing incidence of asthma. For instance, according to the World Health Organization in 2017, approximately 235 Mn people suffered from asthma and in 2015, the disease killed approximately 383 Mn patients around the globe. In addition, increasing healthcare expenditure and growing elderly population along with growing awareness regarding asthma spacers products in developing and under-developed countries are some major factors expected to drive growth of the global asthma spacers market. Moreover, increasing government initiatives that mandatory to use of asthma spacers with metered dose inhalers and rising prevalence of chronic inflammatory disease of the airways are among some other factors expected to drive growth of the global asthma spacers market. Furthermore, increasing technological advancements and rising number of approvals for inhalers and spacers or valve holding chambers is expected to have a positive impact on ...
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Revealed by a mosaic-like structure of the 16S-23SrRNA intergenic spacer region in strain PCC 9901. Phycologia, 52 (4), 333 - 337.. Comte K., Coursin T., Carre-Mlouka A. (2013).. ...
A semiconductor package incorporates spacer strips enabling one or more semiconductor dies having central terminal pads to be stacked on top of one another within the package and reliably wire bonded to an associated substrate without shorting of the bonded wires. Each of the spacer strips comprises a flat, elongated strip of an insulative material that mount at edges of a surface of a die such that they straddle the central terminal pads thereon. The die is electrically connected to the substrate by a plurality of fine conductive wires having a first end bonded to one of the central terminal pad on the die, a second end bonded to a terminal pad on the substrate, and an intermediate portion between the first and second ends that passes transversely across the top surface of one of the spacer strips. The spacer strips have spaced pads or grooves on or in their top surfaces that captivate the individual wires and thereby redistribute the wires and prevent them from contacting the die and each other.
Put bright touches into your jewelry designs with this TierraCast heishi spacer. This small spacer bead features a hexagon nut shape, perfect for using between bigger beads. It will help add interest and complexity to your bead designs. Try it in necklaces, bracelets, and even earrings. Its versatile enough to work with any style, so stock up on this beautiful spacer bead and use it in every project. This spacer bead features a warm copper glow.. TierraCast items are made from high-quality lead-free pewter.. ...
The self-assembly of single-stranded DNA (ssDNA) aptamer-amphiphiles was influenced by the choice of spacer used to link the hydrophobic tail and aptamer headgroup. Aptamer-amphiphiles without spacers or with hydrophilic spacers formed globular micelles while amphiphiles with hydrophobic spacers formed bilay
Calculate the bounded phylogenetic biodiversity metrics: phylogenetic species variability, richness, evenness and clustering for one or multiple samples.
Vol 13: Molecular identification of clinical difficult-to-identify microbes from sequencing 16S ribosomal DNA and internal transcribed spacer 2.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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With most meth injection applications, you would replace the factory charge pipe with an aftermarket version with meth bungs. Traditionally factory charge pipes are also weak, which tend to break when you turn up the boost with your JB4. The B58 charge pipe is robust and does not have the inherent weakness seen in the N55. Additionally, the B58 charge pipe is a pain to replace, having to also remove the entire intake tract ...
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A method of decompressing a facet joint comprises forming an opening in a facet capsule of the facet joint and inserting a spacer device through the opening into the facet capsule.
The forkhead-associated (FHA) domain [1] is a putative nuclear signalling domain found in a variety of otherwise unrelated proteins. The FHA domain comprise approximately 55 to 75 amino acids and contains three highly conserved blocks separated by divergent spacer regions. Currently it has been found in the following proteins: ...
po czym 24.09 przyjeżdżacie, bierzecie naszego podopiecznego (albo dwa) i zabieracie go na wspólną zabawę i wyjątkowo spędzacie razem czas! To niezwykła forma aktywności i ogromna pomoc dla naszych psiaków. Spacer po lesie to dla nich niezwykłe doświadczenie, które da im na chwilę zapomnieć o trudzie życia bez swojego człowieka ...
Evidence from nuclear ribosomal DNA internal transcribed spacer sequences". American Journal of Botany. 80 (6): 711-722. doi: ... or the ribosomal spacers ITS) and cladistic analysis in order to investigate the relationships between the family's different ... Lavin M, Doyle JJ, Palmer JD (1990). "Evolutionary significance of the loss of the chloroplast-DNA inverted repeat in the ... These studies have used morphology, DNA data (the chloroplast intron trnL, the chloroplast genes rbcL and matK, ...
5.8S ribosomal DNA and internal transcribed spacer region analyses". Eukaryotic Cell. 6 (1): 110-6. doi:10.1128/EC.00233-06. ...
1: 3-9. Lennon, PA; Cooper CR; Salkin IF; Lee SB (1994). "Ribosomal DNA internal transcribed spacer analysis supports synonymy ... This synonymy has since been confirmed by phylogenetic analysis of the nuclear ribosomal internal transcribed spacer regions. ... of cultures of Scedosporium-like fungi from clinical cases by careful morphological examination and the evaluation of DNA-DNA ...
Evidence from Nuclear Ribosomal DNA Internal Transcribed Spacer Sequences". Am J Bot. 80 (6): 711-722. doi:10.2307/2445441. ... Phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndhF sequences". Plant Syst ... and its Major Subgroups Based on Nuclear Ribosomal DNA ITS and Chloroplast DNA trnL Intron Data". Syst Bot. 24 (3): 409-437. ... molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA". J Agric Food Chem. 51 (23): 6709-6714. doi: ...
... inferred from internal transcribed spacer ribosomal DNA sequences". Canadian Journal of Botany. 77 (1): 150-168. doi:10.1139/ ...
... based on nuclear ribosomal DNA internal transcribed spacer region sequences". American Journal of Botany. Botanical Society of ...
Saghai-Maroof, M.A.; Soliman, K.M.; Jorgensen, R.A. (1984). "Ribosomal DNA spacer-length polymorphisms in barley: mendelian ... Cluster, P.D.; Allard, R.W. (1995). "Evolution of ribosomal DNA (rDNA) genetic structure in colonial Californian populations of ... Wagner, D.B.; Furnier, G.R.; Saghai-Maroof, M.A.; Williams, S.M.; Dancik, B.P.; Allard, R.W. (1987). "Chloroplast DNA ... Neale, D.B.; Saghai-Maroof, M.A.; Allard, R.W.; Zhang, Q.; Jorgensen, R.A. (1988). "Chloroplast DNA diversity in populations of ...
"Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi". Proceedings of the ... Highly conserved DNA sequences are thought to have functional value, although the role for many highly conserved non-coding DNA ... such as ribosomal RNAs, tRNAs and ribosomal proteins are also universally conserved. Sets of conserved sequences are often used ... "Characterization of Bacterial and Fungal Soil Communities by Automated Ribosomal Intergenic Spacer Analysis Fingerprints: ...
Kiss L (July 2012). "Limits of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as species barcodes for Fungi ... The amplification of DNA from environmental samples using general or group-specific primers leads to a mix of genetic material ... 2013), the D1-D3 region of 28S ribosomal RNA genes, may not allow the low taxonomic level classification of the ITS, but ... The development of molecular ecology is also closely related to the use of DNA microarrays, which allows for the simultaneous ...
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ... 1985). "Molecular analysis of the heterogeneity region of the human ribosomal spacer". J. Mol. Biol. 183 (2): 213-223. doi: ...
... based on nuclear ribosomal DNA internal transcribed spacer sequences". American Journal of Botany. 81 (9): 1149-1160. doi: ... During this period, his laboratory methodology changed from working with rRNA to DNA using the polymerase chain reaction (PCR) ...
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... RNA, ribosomal 5, also known as RNR5, is a human gene. Genes for ribosomal RNA are clustered on the short arms of chromosomes ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ...
... using the first internal transcribed spacer and the D1-D3 expansion segments of 28S ribosomal DNA. Journal of Nematology 40(3 ...
... evidence from nuclear ribosomal DNA internal transcribed spacer sequences" (PDF). American Journal of Botany. 87 (1): 76-95. ...
High-resolution phylogeny for Helianthus (Asteraceae) using the 18S-26S ribosomal DNA external transcribed spacer. American ...
Won H, Renner SS: The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum. Molecular Phylogenetics ...
... based on internal transcribed spacer sequences of ribosomal DNA". Korean Journal of Genetics. 20 =issue=2: 109-116. The idea ... conducted the phylogenetic analysis using sequences from both rbcL gene and trnL-trnF intergenic spacer of chloroplast DNA and ... September 2002). "Phylogenetic Analysis of Korean Prunus ( Rosaceae ) Based on ITS Sequences of Nuclear Ribosomal DNA". Gene & ... markers and sequence analysis of two chloroplast DNA genes, rpl16 and trnL-trnF spacer and showed that king cherry can be ...
Phylogenetic relationships in Helianthus (Asteraceae) based on nuclear ribosomal DNA internal transcribed spacer region ...
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... Her cladistic analysis combined comparisons of the sequences of the internal transcribed spacer in the non-functional RNA with ...
Based on Nuclear Ribosomal Internal Transcribed Spacer and Chloroplast trnL-F Sequences. In Aliso: A Journal of Systematic and ... based on chloroplast and nuclear DNA sequences. Telopea, 13(1-2), 115-142. Soreng, R. J., Peterson, P. M., Romaschenko, K., ...
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... The cladistic analysis combined comparison of sequences from the internal transcribed spacer in the non-functional RNA with ...
... of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer ... DNA sequence and mechanism of transfer". Journal of Bacteriology. 182 (1): 81-90. doi:10.1128/jb.182.1.81-90.2000. PMC 94243. ...
Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer ...
... and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer ... DNA homology to the closely related species Mycobacterium senegalense. Both species, share an identical 5' 16S rDNA sequence. ...
... based on internal transcribed spacers and 5.8S nuclear ribosomal DNA". Biochemical Systematics and Ecology. 25 (5): 445. doi: ...
"Ribosomal DNA internal transcribed spacer 2 sequence analysis and phylogenetic comparison of seven cockroach species in ...
... of nuclear ribosomal DNA. American Journal of Botany 86(4), 590-607. ... 1999). Phylogenetic relationships in Lupinus (Fabaceae: Papilionoideae) based on internal transcribed spacer sequences (ITS) ... 2005). 2C DNA variation and relationships among New World species of the genus Lupinus (Fabaceae). Plant Systematics and ...
He was the first to report the sequence of the spacer of genes encoding ribosomal RNAs of a dicotyledon in 1988 and to show the ... DNA, after cloning and amplification in Escherichia coli, was infectious and identical to the original DNA when inoculated to a ... Structural and transcriptional characterization of the external spacer of a ribosomal RNA nuclear gene from a higher plant », ... He was among the first in France to clone and sequence plant DNA in the early 1980s, and, as such, he helped to organize the ...
The genus Naegleria's ribosomal DNA (rDNA) consists of an extrachromosomal plasmid of which about 4000 exist in each cell. ... Species can also be distinguished by their internal transcribed spacers type 2 (ITS2) sequences. One species of Naegleria is ... New species are often defined by ribosomal DNA sequences. The unicellular organism's cytoplasm has distinct separations of an ...
... based on sequences from the internal transcribed spacers (ITS) of nuclear ribosomal DNA". American Journal of Botany. 85 (12): ... after analyzing and matching the internal transcribed spacers of nuclear ribosomal DNA from many Seriphidium and Artemisia ... Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS)". Plant Biology. 5 (3): 274-84. doi: ... Chloroplast and ribosomal DNA sequence analysis in 2011 supported monophyly with two clades, one of which includes some North ...
... based on nuclear ribosomal DNA ITS sequences" (PDF). Aliso. 22: 372-395. Retrieved 13 October 2015.. ... based on nuclear ribosomal internal transcribed spacer and chloroplast rps16 sequences, focusing on the inclusion of species ... ramosum as assessed by random amplified polymorphic DNA (RAPD). Retrieved 14 October 2015.. in Zeder et al (2006, Chapter 10. ...
Monophyly of aneuploid Astragalus: Evidence from nuclear ribosomal DNA internal transcribed spacer sequences. American J. Bot. ... Evolutionary significance of the loss of the chloroplast--DNA inverted repeat in the Leguminosae subfamily Papilionoideae. ...
Rowan R, Powers DA (1992) Ribosomal-RNA sequences and the diversity of symbiotic dinoflagellates (zooxanthellae). Proc Natl ... Utilizáronse datos de Internal transcribed spacer region 2 (ITS2) (sensu LaJeunesse 2002) como unha aproximación para a ... Genetic variation in Symbiodinium isolates from giant clams based on random-amplified-polymorphic DNA (RAPD) patterns. Mar Bio ... ribosomal e nuclear de copia única. O uso de múltiples marcadores, xunto cunha clasificación filoxenética xerárquica ...
Achenbach-Richter, L; Woese CR (1988). "The ribosomal gene spacer region in archaebacteria". Syst. Appl. Microbiol. 10: 211-214 ... "DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA". Int. J. Syst. Evol. Microbiol. 50: 1095-1102 ... Winker, S; Woese CR (1991). "A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA ... Hansmann, S; Martin W (2000). "Phylogeny of 33 ribosomal and six other proteins encoded in an ancient gene cluster that is ...
"Deriving ribosomal binding site (RBS) statistical models from unannotated DNA sequences and the use of the RBS model for N- ... The composition of nucleotides in the spacer region itself was also found to affect the rate of translation initiation in one ... "DNA Research. 15 (6): 387-396. doi:10.1093/dnares/dsn027. ISSN 1340-2838. PMC 2608843 . PMID 18940874.. ... A ribosome binding site, or ribosomal binding site (RBS), is a sequence of nucleotides upstream of the start codon of an mRNA ...
In one application an artificial DNA catalyst was prepared by attaching a copper ion to it through a spacer.[42] The copper - ... "Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena". Cell. 31 (1): ... Other uses of DNA in chemistry are in DNA-templated synthesis, Enantioselective catalysis,[43] DNA nanowires and DNA computing. ... Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of performing a ...
"Mitochondrial and ribosomal internal transcribed spacer (ITS1) diversity of the bed bug Cimex lectularius L. (Heteroptera: ... A population genetics study of bed bugs in the United States, Canada, and Australia using a mitochondrial DNA marker found high ... In contrast to the high amount of genetic variation observed with the mitochondrial DNA marker, no genetic variation in a ...
A phylogenetic analysis of Prunus and the Amygdaloideae (Rosaceae) using ITS sequences of nuclear ribosomal DNA. „Am. J. Bot. ... Using rp116-rp114 Spacer Sequences of Chloroplast DNA. „Journal of the Japanese Society for Horticultural Science". 75, 1, s. ... Joey Shaw, Randall L. Small: Chloroplast DNA phylogeny and phylogeography of the North American plums (Prunus subgenus Prunus ...
Achenbach-Richter, L; Woese CR (1988). "The ribosomal gene spacer region in archaebacteria". Syst. Appl. Microbiol. 10: 211-214 ... "DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA". Int. J. Syst. Evol. Microbiol. 50: 1095-1102 ... Keswani, J; Whitman WB (2001). "Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes". Int. J. Syst ... Winker, S; Woese CR (1991). "A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA ...
... and Related Genera Based on DNA Sequences from ITS Nuclear Ribosomal DNA and Plastid trnL Intron and trnL-F Intergenic Spacers" ...
Salini Konikkat: Dynamic Remodeling Events Drive the Removal of the ITS2 Spacer Sequence During Assembly of 60S Ribosomal ... The sequence of DNA, which encodes the sequence of the amino acids in a protein, is copied into a messenger RNA chain. It may ... Each subunit is composed of one or more ribosomal RNA (rRNA) molecules and a variety of ribosomal proteins (r-protein or ... The ribosomal proteins and rRNAs are arranged into two distinct ribosomal pieces of different size, known generally as the ...
... of nuclear ribosomal DNA and the trnL/F region of Chloroplast DNA». Int. J. Plant Sci. 164 (2): 197-211. ISSN 1537-5315.. ... 2003). «The phylogeny of Rosoideae (Rosaceae) based on sequences of the internal transcribed spacers (ITS) ... C. Dobeš og J. Paule (2010). «A comprehensive chloroplast DNA-based phylogeny of the genus Potentilla (Rosaceae): implications ...
... nuclear ribosomal and nuclear low-copy DNA". Molecular Phylogenetics and Evolution. 54: 457-471. doi:10.1016/j.ympev.2009.09. ... Monophyly of the genus is supported by nuclear ribosomal internal transcribed spacer (ITS) and cpDNA.[3] ...
... of nuclear ribosomal DNA. American Journal of Botany 86(4), 590-607. ... 1999). Phylogenetic relationships in Lupinus (Fabaceae: Papilionoideae) based on internal transcribed spacer sequences (ITS) ... 2005). 2C DNA variation and relationships among New World species of the genus Lupinus (Fabaceae). Plant Systematics and ...
Some single-celled organisms have much more DNA than humans, for reasons that remain unclear (see non-coding DNA and C-value ... "Psyllid endosymbionts exhibit patterns of co-speciation with hosts and destabilizing substitutions in ribosomal RNA". Insect ... dispersed in the ancestor gene but aggregated and the negative relation between number of lost genes and length of the spacers. ... I. DNA-content and chromosome sets in various species of Cyprinidae". Humangenetik. 7 (3): 240-244. doi:10.1007/BF00273173. ...
... based on plastid matK and nuclear ribosomal spacer (ITS) DNA sequences and morphology" (PDF). Plant Systematics and Evolution. ...
... based on sequences from the internal transcribed spacers (ITS) of nuclear ribosomal DNA". American Journal of Botany. 85 (12): ... Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS)". Plant Biology. 5 (3): 274-284. doi: ... after analyzing and matching the internal transcribed spacers of nuclear ribosomal DNA from many Seriphidium and Artemisia ... Dracunculus (Asteraceae) based on ribosomal and chloroplast DNA sequences". Taxon. 60 (3): 691-704. doi:10.1002/tax.603006.. ...
... of Section Sinomartagon in Genus Lilium Using Sequences of the Internal Transcribed Spacer Region in Nuclear Ribosomal DNA". 育種 ... platyphyllum Baker and L. rubellum Baker Based on Three Spacer Regions in Chloroplast DNA". 育種学雑誌 Breeding Science. 52 (3): 207 ...
... using ITS sequences of nuclear ribosomal DNA". American Journal of Botany. 88 (1): 150-160. doi:10.2307/2657135. JSTOR 2657135 ... as Determined by Sequence Analysis of ITS and the Chloroplast trnL-trnF Spacer DNA". Systematic Botany. 26 (4): 797-807. JSTOR ... Another recent DNA study[10] found two clades: Prunus-Maddenia, with Maddenia basal within Prunus, and Exochorda-Oemleria- ... A recent DNA study of 48 species concluded that Prunus is monophyletic and is descended from some Eurasian ancestor.[9] ...
... based on plastid matK and nuclear ribosomal spacer (ITS) DNA sequences and morphology". Plant Systematics and Evolution. 246 (3 ... This study used nuclear encoded nrITS (Nuclear ribosomal internal transcribed spacer), and plastid encoded matK (Maturase K), ... By contrast another study performed at the same time, using both nuclear and chloroplast DNA, but limited to the 14 species ... of Anatolia inferred from multiple nuclear and chloroplast DNA regions". Turkish Journal of Botany. 37: 993-1007. doi:10.3906/ ...
... and Species Identification of Dermatophyte Strains Based on DNA Sequences of Nuclear Ribosomal Internal Transcribed Spacer 1 ...
... based on the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences". New Phytol. 170 (2): 411-20. doi: ... using three chloroplast DNA data sets". Genome. 45 (6): 991-1002. doi:10.1139/g02-065. PMID 12502243.. ... "Site of Einkorn Wheat Domestication Identified by DNA Fingerprinting". Science. 278 (5341): 1312-1314. doi:10.1126/science. ... "Molecular characterization of a diagnostic DNA marker for domesticated tetraploid wheat provides evidence for gene flow from ...
"Ribosomal DNA in diploid and polyploid Setaria (Poaceae) species: number and distribution". Comp Cytogenet. 9 (4): 645-60. doi: ... "Definition of Eight Mulberry Species in the Genus Morus by Internal Transcribed Spacer-Based Phylogeny". PLoS ONE. 10 (8): ... Mahendran B, Ghosh SK, Kundu SC (2006). "Molecular phylogeny of silk-producing insects based on 16S ribosomal RNA and ... Wang W, Lan H (2000). "Rapid and parallel chromosomal number reductions in muntjac deer inferred from mitochondrial DNA ...
... while base-pairing between DNA and incoming nucleotides provides the mechanism through which DNA polymerase replicates DNA and ... Cockburn AF, Newkirk MJ, Firtel RA (December 1976). "Organization of the ribosomal RNA genes of Dictyostelium discoideum: ... mapping of the nontranscribed spacer regions". Cell. 9 (4 Pt 1): 605-13. doi:10.1016/0092-8674(76)90043-X. PMID 1034500.. ... Zhurkin VB, Tolstorukov MY, Xu F, Colasanti AV, Olson WK (2005). Sequence-Dependent Variability of B-DNA. DNA Conformation and ...
Phylogenetic analysis of Leucojum and Galanthus (Amaryllidaceae) based on plastid matK and nuclear ribosomal spacer (ITS) DNA ...
ribosomal proteins. tRNAs. nicotiana tabacum. edit · image. Chloroplast DNA Interactive gene map of chloroplast DNA from ... These large protein complexes may act as spacers between the sheets of stromal thylakoids.[125] ... As in prokaryotes, genes in chloroplast DNA are organized into operons.[24] Unlike prokaryotic DNA molecules, chloroplast DNA ... Green DNA: BIOSCIENCE EXPLAINED.. *^ a b c d e Clegg, M. T.; Gaut, BS; Learn Jr, GH; Morton, BR (1994). "Rates and Patterns of ...
Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Conrad L. Schoch, Keith ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi ...
We have therefore analysed E. gracilis strain Z 16S-23S spacer DNA at the nucleotide level, hoping this would allow ... 9). Total chloroplast tRNA hybridizes to fragments of rDNA9 and it was suggested that the 16S-23S spacer region contains tRNA ... The latter is suggested from the 189 bases which have been sequenced from the 2.4-kilobase pair rDNA spacer from maize ... The rRNA genes are located within three tandemly repeated DNA regions of approximately 5.6 kilobase pairs each6-8 and the ...
Ribosomal DNA evolution at the population level: nucleotide variation in intergenic spacer arrays of Daphnia pulex.. T J Crease ... Ribosomal DNA evolution at the population level: nucleotide variation in intergenic spacer arrays of Daphnia pulex.. T J Crease ... Ribosomal DNA evolution at the population level: nucleotide variation in intergenic spacer arrays of Daphnia pulex.. T J Crease ... Ribosomal DNA evolution at the population level: nucleotide variation in intergenic spacer arrays of Daphnia pulex. ...
... of 36 specimens of Japanese Amanitaspecies was studied based on nucleotide sequences of the internal transcribed spacer (ITS) ... of JapaneseAmanita species based on nucleotide sequences of the internal transcribed spacer region of nuclear ribosomal DNA. ... Separate structural elements within internal transcribed spacer 1 ofSaccharomyces cerevisiae precursor ribosomal RNA direct the ... region of nuclear ribosomal DNA. The phylogenetic tree obtained supported the traditional classification systems of Bas (1969) ...
The ribosomal DNA region spanning the second internal transcribed spacer (ITS-2) was amplified from genomic DNA by PCR, ... Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for ...
Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species. Takashi Sugita, Masamitsu Nakajima ... Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species ... Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species ... Sequence Analysis of the Ribosomal DNA Intergenic Spacer 1 Regions of Trichosporon Species ...
... the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). This study identified six ... The shortest two IGS types, A and B, lacked the transcription initiation site, non-transcribed spacer, and external transcribed ... of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer ... but the external transcribed spacers of these four IGSs were quite similar in length. The 45S IGSs were found to follow ...
The 16S/23S ribosomal spacer region as a target for DNA probes to identify eubacteria. PCR Methods Appl.1:51-56. ... The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of ... Identification of Clinically Relevant Viridans Group Streptococci by Sequence Analysis of the 16S-23S Ribosomal DNA Spacer ... Identification of Clinically Relevant Viridans Group Streptococci by Sequence Analysis of the 16S-23S Ribosomal DNA Spacer ...
To investigate the monophyly of Potentillaand the phylogenetic relationships of associated genera we used nuclear ribosomal ... Phylogenetic analysis ofPotentilla using DNA sequences of nuclear ribosomal internal transcribed spacers (ITS), and ... Nucleotide sequences of the 5.8S rRNA gene and internal transcribed spacer regions in carrot and broad bean ribosomal DNA. - J ... evidence from sequences of the internal transcribed spacers of nuclear ribosomal DNA and its congruence with morphology. - Amer ...
An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence ... An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence ... An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence ... An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence ...
Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of ... The nuclear ribosomal small subunit has poor species-level resolution in fungi. ITS will be formally proposed for adoption as ... The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some ... Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein- ...
Identification of Serratia marcescens on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms ... Identification of Serratia marcescens on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. J ... Identification of Serratia marcescens on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. ... We used primers to amplify spacer regions between the 16S and 23S genes in the prokaryotic rDNA genetic loci. When the spacer ...
An automated ribosomal intergenic spacer analysis of fresh and incubated fecal DNA confirmed association between microbial ... DNA Extraction and Automated Ribosomal Intergenic Spacer Analysis (ARISA). Fresh and incubated feces samples used to analyze ... An automated ribosomal intergenic spacer analysis of fresh and incubated fecal DNA confirmed association between microbial ... VOCs also underwent DNA extraction and microbial fingerprinting by means of automated ribosomal intergenic spacer analysis ( ...
Sequence analysis of the ribosomal DNA internal transcribed spacer 1 in populations of Lepthyphantes tenuis (Araneae: ...
... identified the species using a ribosomal DNA (r-DNA) second internal transcribed spacer (ITS2) based allele specific polymerase ... Ribosomal DNA of one individual from Assam state did not amplify in the ASPCR, possessed 786 base pair long ITS2 sequence and ... Spatial distribution and r-DNA second internal transcribed spacer characterization of Anopheles dirus (Diptera: Culicidae) ...
DNA, Ribosomal Spacer Grant support * BB/M011755/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom ... new analysis pipelines offer additional approaches for taxonomic assertions based on ribosomal internal transcribed spacer ...
Populations of the south-west Pacific malaria vector Anopheles farauti s.s. revealed by ribosomal DNA transcribed spacer ... revealed by ribosomal DNA transcribed spacer polymorphisms . Opens in a new window. ...
Vol 13: Molecular identification of clinical difficult-to-identify microbes from sequencing 16S ribosomal DNA and internal ... transcribed spacer 2.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. ... Therefore, the ability of 16S ribosomal DNA 16S rDNA and internal transcribed spacer 2 ITS2 sequencing to identify these - ... identification of clinical difficult-to-identify microbes from sequencing 16S ribosomal DNA and internal transcribed spacer 2. ...
DNA, Ribosomal Spacer / genetics * Fatty Acids / analysis * France * Legionella / classification* * Legionella / genetics* ... Genotypic profiles generated by randomly amplified polymorphic DNA and 16S-23S rDNA spacer region PCR analyses were unique for ... DNA-DNA relatedness values of strains Montbéliard A1T and Gréoux 11 D13T to each other and to other Legionella type strains ...
DNADNA Mutational Analysis • DNA Primers • DNA, Chloroplast • DNA, Plant • DNA, RibosomalDNA, Ribosomal Spacer • ... We utilize DNA sequence data from the nuclear, chloroplast, and mitochondrial genomes to infer historical processes of ... DNA • Soil • Species Specificity • Sphagnopsida • Transcriptome • Tropical Climate. Curriculum Vitae. Current Ph.D. Students * ...
DNA, Fungal • DNA, Intergenic • DNA, Mitochondrial • DNA, RibosomalDNA, Ribosomal Spacer • Ecosystem • Endonucleases • ... Ribosomal • RNA, Ribosomal, 18S • RNA, Ribosomal, 28S • Rosacea • Sequence Alignment • Sequence Analysis, DNA • Sequence ... Rydholm, C; Dyer, PS; Lutzoni, F, DNA sequence characterization and molecular evolution of MAT1 and MAT2 mating-type loci of ... Lutzoni, F; Wagner, P; Reeb, V; Zoller, S, Integrating ambiguously aligned regions of DNA sequences in phylogenetic analyses ...
... mitochondrial 16S ribosomal DNA, cytochrome oxidase subunit I, and the internal transcribed spacer of ribosomal DNA) showed the ... Internal Transcribed Spacer Region of Ribosomal DNA. Several Zoanthus specimens (1569, 1570, 1571, 1572, 1575, 1577, and 1578) ... DNA Extraction, PCR Reactions, and DNA Sequencing. Genomic DNA was extracted from portions of specimens by following a ... internal transcribed spacer of ribosomal DNA (ITS-rDNA) sequences. Novel sequences from specimens in this study represented in ...
Mutation scanning-coupled sequencing of nuclear ribosomal DNA spacers as a tool for the specific identification of different ...
... of nuclear ribosomal DNA and the trnL/F region of chloroplast DNA ... based on sequences of the internal transcribed spacers (ITS) ... The phylogeny of Rosoideae (Rosaceae) based on sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA and ... The analysis of these new data, along with previously used data from the nuclear ribosomal internal transcribed spacers (ITS), ...
The DNA sequence of a highly conserved region of these repeats was used to synthesize an oligonucleotide probe specific to A. ... Both A. aquasalis-specific clones were from the intergenic spacer region of the ribosomal RNA (rRNA) cistron. Upon digestion ... Analysis of the DNA sequence of this fragment revealed four tandemly repeated 36-bp units. Three of these repeat units were ... aquasalis than to DNA from A. benarrochi Gabaldon, Cova Garcia, and Lopez, A. konderi Galvao and Damasceno, A. nuneztovari ...
We report here two highly reliable ribosomal DNA-based single nucleotide polymorphism (SNP) markers, CxpG2T and CxpA2d, for ... Analysis of a ribosomal DNA intergenic spacer region from the yellow fever mosquito, Aedes aegypti . Insect Mol Biol 7: 19-29.[ ... Variation in ribosomal DNA intergenic spacers among populations of Anopheles albimanus in South and Central America. Am J Trop ... Louis encephalitis virus based on interspecies sequence variation in ribosomal DNA spacers. Am J Trop Med Hyg 53: 105-109.. [ ...
0 (DNA, Helminth); 0 (DNA, Ribosomal Spacer); 0 (RNA, Ribosomal, 18S); EC 1.9.3.1 (Electron Transport Complex IV). ... DNA de Helmintos/qu mica. DNA de Helmintos/isolamento & purifica o. DNA Espa ador Riboss mico/qu mica. Dictyocaulus/anatomia & ... DNA Mitocondrial/gen tica. Europa (Continente). Fran a. Genoma Mitocondrial. Filogenia. An lise de Sequ ncia de DNA. ... and internal transcribed spacer 2 (ITS2) regions of the ribosomal gene array as well as the mitochondrial cytochrome c oxidase ...
0 (DNA, Ribosomal Spacer). [Em] M s de entrada:. 1709. [Cu] Atualiza o por classe:. 170918. ... DNA Espa ador Riboss mico/gen tica. Feminino. Filariose/epidemiologia. Filariose/parasitologia. Filarioidea/isolamento & ... Nanocurcumin: a novel antifilarial agent with DNA topoisomerase II inhibitory activity.. [So] Source:. J Drug Target;22(5):395- ... METHODS: We propose the development of a PCR assay linked with DNA pyrosequencing as a rapid tool to identify W. bancrofti, B. ...
Evidence from nuclear ribosomal DNA internal transcribed spacer sequences. American Journal of Botany. 80 (6): 711-722. doi: ... or the ribosomal spacers ITS) and cladistic analysis in order to investigate the relationships between the familys different ... These studies have used morphology, DNA data (the chloroplast intron trnL, the chloroplast genes rbcL and matK, ... Lavin M, Doyle JJ, Palmer JD (1990). Evolutionary significance of the loss of the chloroplast-DNA inverted repeat in the ...
DNA, Fungal / chemistry, genetics. DNA, Ribosomal / chemistry, genetics. DNA, Ribosomal Spacer / chemistry, genetics. ... 0/Antifungal Agents; 0/DNA, Fungal; 0/DNA, Ribosomal; 0/DNA, Ribosomal Spacer; 7XU7A7DROE/Amphotericin B ...
  • An automated ribosomal intergenic spacer analysis of fresh and incubated fecal DNA confirmed association between microbial fingerprinting and short chain VFAs, phenolic and sulfuric VOCs. (frontiersin.org)
  • For more results try searching for Automated Ribosomal Intergenic Spacer Analysis (ARISA) across all experimental services. (scienceexchange.com)
  • evidence from sequences of the internal transcribed spacers of nuclear ribosomal DNA and its congruence with morphology. (springer.com)
  • Phylogenetic analyses of sequence data from the transcribed spacers of nuclear ribosomal DNA (ETS, ITS) indicate that W. sect. (ingentaconnect.com)
  • Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). (pnas.org)
  • The rRNA genes are located within three tandemly repeated DNA regions of approximately 5.6 kilobase pairs each 6-8 and the arrangement of the structural genes within each repeat follows the prokaryotic pattern, being 5′-16S-23S-5S-3′ (ref. 9). (nature.com)
  • We have therefore analysed E. gracilis strain Z 16S-23S spacer DNA at the nucleotide level, hoping this would allow identification of tRNA genes together with the processing sites of the respective primary transcripts. (nature.com)
  • Flanking sequences, coding for the 3′-terminal region of 16S rRNA and for the 5′-terminal region of 23S rRNA have also been sequenced, to compare the drift rates between the spacer and its adjacent structural genes. (nature.com)
  • We determined the sequence of the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes, in 25 species of the genus Trichosporon . (asm.org)
  • The 45S rDNA of flowering plants includes the 5.8S, 18S and 25S rDNA genes, the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). (mdpi.com)
  • We used primers to amplify spacer regions between the 16S and 23S genes in the prokaryotic rDNA genetic loci. (docphin.com)
  • The differences in the evolutionary rates in the ribosomal genes of dinoflagellates, especially SSU rDNA and also in the domains of the LSU rDNA, has yielded phylogenetic trees with a characteristic structure: a large group of short branched sequences, the so-called Gymnodiniales, Prorocentrales and Peridiniales complex (GPP complex), and a group with longer branches including Gonyaulacales and other taxa (Saunders et al. (scribd.com)
  • Of these 151 core genes, 39 were putative genes encoding the 30S and 50S ribosomal subunits, whilst among recognized cell division genes, only one gene, the major ftsZ , was present. (uoguelph.ca)
  • The nucleolus is a nuclear substructure where the genes for three of the four ribosomal ribonucleic acids (rRNAs) are transcribed and where ribosomal subunits are assembled. (els.net)
  • The intergenic spacer of the ribosomal genes in eukaryotes (IGS) contains duplications of the core transcription promoter. (biomedcentral.com)
  • Instead, multiple genes associated with DNA replication and modulation of chromatin structure were isolated. (asm.org)
  • DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA. (umassmed.edu)
  • A comparison of the ribosomal DNA's of Xenopus laevis and Xenopus mulleri: the evolution of tandem genes. (xenbase.org)
  • Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA. (xenbase.org)
  • The genes for ribosomal RNA during oögenesis. (xenbase.org)
  • The amplification of ribosomal RNA genes involves a rolling circle intermediate. (xenbase.org)
  • Chromosomal conservation and sequence diversity of ribosomal RNA genes of two distant Oryza species. (semanticscholar.org)
  • Transcribed nucleolar chomatin, including the spacer regions interspersed between the rRNA genes, is different from the bulk of nontranscribed chromatin in that the DNA of these regions appears to be in an extended (B) conformation when examined by electron microscopy. (uni-wuerzburg.de)
  • Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. (pnas.org)
  • The Internal Transcriber Spacer region of fungal ribosomal DNA was utilized for identification purposes. (hindawi.com)
  • While exploring this diversity, we discovered that only half of the known marine fungal species have a publicly available DNA locus, which we hypothesize will likely hinder accurate high-throughput sequencing classification in the future, as it does currently. (degruyter.com)
  • Identification of the fungal isolate was based on nuclear ribosomal DNA internal transcribed spacer (ITS) identities. (rsc.org)
  • Ribosomal RNA gene Internal Transcribed Spacer (ITS) regions were sequenced from Puccinia carduorum, a rust fungal pathogen of Carduus thoermeri (musk thistle). (usda.gov)
  • The internal transcribed spacer (ITS) region of the ribosomal DNA is the conventional marker region for fungal community studies. (biomedcentral.com)
  • High-throughput sequencing of DNA barcode marker regions, namely the bacterial 16S rRNA gene or fungal internal transcribed spacer (ITS) ribosomal regions, have allowed researchers to characterize complex microbial communities at a depth not previously possible with culture-based methods. (biomedcentral.com)
  • Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. (pnas.org)
  • Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeri. (nih.gov)
  • Single-strand restriction fragment length polymorphism analysis of the second internal transcribed spacer (ribosomal DNA) for six species of Eimeria from chickens in Australia. (nih.gov)
  • The ribosomal DNA region spanning the second internal transcribed spacer (ITS-2) was amplified from genomic DNA by PCR, digested separately with three restriction endonucleases (CfoI, Sau3AI and TaqI) and the fragments separated by denaturing gel electrophoresis. (nih.gov)
  • Comparative sequence analysis suggested that the divergence of IGS 1 sequences has been greater than that of the internal transcribed spacer regions. (asm.org)
  • Two other regions exist in each repeat: the internal transcribed spacer (ITS) region and the intergenic spacer (IGS) region (Fig. 1 ). (asm.org)
  • To investigate the monophyly of Potentilla and the phylogenetic relationships of associated genera we used nuclear ribosomal internal transcribed spacer (ITS) DNA sequences. (springer.com)
  • Spatial distribution and r-DNA second internal transcribed spacer characterization of Anopheles dirus (Diptera: Culicidae) complex species in north. (nih.gov)
  • dirus s.l. from 27 forested/forest fringed locations spread across the seven north-eastern states, identified the species using a ribosomal DNA (r-DNA) second internal transcribed spacer (ITS2) based allele specific polymerase chain reaction (ASPCR) method and sequenced the ITS2 locus in a sub set of mosquitoes. (nih.gov)
  • MGnify's new analysis pipelines offer additional approaches for taxonomic assertions based on ribosomal internal transcribed spacer regions (ITS1/2) and expanded protein functional annotations. (nih.gov)
  • Vol 13: Molecular identification of clinical difficult-to-identify microbes from sequencing 16S ribosomal DNA and internal transcribed spacer 2. (duhnnae.com)
  • Therefore, the ability of 16S ribosomal DNA 16S rDNA and internal transcribed spacer 2 ITS2 sequencing to identify these -difficult-to-identify- bacteria and fungi was assessed in this study. (duhnnae.com)
  • Surprisingly, the results from analyses utilizing three DNA markers (mitochondrial 16S ribosomal DNA, cytochrome oxidase subunit I, and the internal transcribed spacer of ribosomal DNA) showed the presence of at least eleven species, of which up to four appear undescribed. (hindawi.com)
  • The analysis of these new data, along with previously used data from the nuclear ribosomal internal transcribed spacers (ITS), significantly increased resolution as well as confidence for Rosoideae phylogeny. (duke.edu)
  • Polymerase chain reaction (PCR) products of the internal transcribed spacer 1 (ITS1) region were then cloned into the pGEM-T vector, and the recombinant plasmids were used as templates for sequencing. (bireme.br)
  • The absence of all potential vectors of human Plas- an internal transcribed spacer 2 gene sequence analysis modium spp. (cdc.gov)
  • X97632 M.kansasii DNA for internal transcribed spacer. (atcc.org)
  • Availability of sequences of the internal transcribed spacers (ITS) and cytochrome oxidase I (COI) show a strong bias towards cultivable species. (scribd.com)
  • Won H, Renner SS: The internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum . (wikipedia.org)
  • Scrophulariaceae): Evidence from the Internal Transcribed Spacer (ITS) Sequences of Nuclear Ribosomal DNA. (uaf.edu)
  • Internal transcribed spacer (ITS) regions 1 and 2, including the 5.8S ribosomal DNA were sequenced for isolate 02-059. (usda.gov)
  • The outcome of sequencing the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) indicated that it belongs to G. frondosa and provided an insight to the phylogenetic relationships within the genus. (mdpi.com)
  • A phylogeny of euapioids was inferred from 1194 nuclear ribosomal DNA internal transcribed spacer (nrDNA ITS) sequences using Bayesian methods. (ingentaconnect.com)
  • Accepted methods include sequencing of the D1-D2 region of the 28s ribosomal DNA (rDNA) or the internal transcribed spacer (ITS) region of rDNA. (cdc.gov)
  • Phylogenetic analyses of Nitella subgenus Tieffallenia (Charales, Charophyceae) using nuclear ribosomal DNA internal transcribed spacer sequences. (algaebase.org)
  • Taxonomic reexamination of 17 species of Nitella subgenus Tieffallenia (Charales, Charophyceae) based on internal morphology of the oospore wall and multiple DNA marker sequences. (algaebase.org)
  • Based on sequence of the internal transcribed spacer (ITS) region of the ribosomal DNA, 11 Phytophthora spp. (apsnet.org)
  • Phylogenetic analysis of Stylosanthes (Fabaceae) based on the internal transcribed spacer region (ITS) of nuclear ribosomal DNA. (scielo.br)
  • ITS (internal transcribed spacers of the rDNA) and LSU (large subunit of the rDNA) of the fungi were sequenced and compared with reference sequences in NCBI for identification. (ncl.edu.tw)
  • DNA sequences of the internal transcribed spacer (ITS) region of the ribosomal RNA gene complex matched those of P. atlanticus . (int-res.com)
  • Internal transcribed spacers (ITS) of Trypanosoma rangeli ribosomal DNA (rDNA): a useful marker for inter-specific differentiation. (fiocruz.br)
  • Transcription of the CHO spacer promoter was achieved by the same protein factors, C and D, that catalyzed transcription of the gene promoter, and these factors bound stably to the CHO spacer promoter in a preinitiation complex, just as they did to the gene promoter. (asm.org)
  • There was also only limited homology between the spacer and gene promoter regions, but despite this the two kinds of initiation regions were organized similarly, both consisting of an essential core promoter domain and a stimulatory domain that extended upstream to approximately residue -135. (asm.org)
  • When a cloned 6 kb Eco RI-Sal I fragement of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. (nih.gov)
  • L42262 Mycobacterium kansasii 16S ribosomal RNA (16S rRNA) gene and 23S ribosomal RNA (23S rRNA) gene. (atcc.org)
  • RISA involves PCR amplification of a region of the rRNA gene operon between the small (16S) and large (23S) subunits called the intergenic spacer region ISR. (wikipedia.org)
  • Any of the DNA in between gene-coding DNA, including untranslated regions, 5' and 3' flanking regions, INTRONS, non-functional pseudogenes, and non-functional repetitive sequences. (umassmed.edu)
  • The observation that mop1 affects paramutation at multiple loci, despite major differences between these loci in their gene structure, correlations with DNA methylation, and stability of the paramutant state, suggests that a common mechanism underlies paramutation. (plantcell.org)
  • Epigenetics refers to altered gene expression associated with alternative chromatin or methylation states (or both) superimposed on an unchanged primary DNA sequence. (plantcell.org)
  • Gene linkage by RNA-DNA hybridization. (xenbase.org)
  • Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA. (xenbase.org)
  • Ribosomal RNA gene amplification by rolling circles. (xenbase.org)
  • Nucleotide sequence of the intergenic spacer and the 18S ribosomal RNA gene from mustard (Sinapis alba). (semanticscholar.org)
  • Nucleotide sequence of the 25S-18S ribosomal gene spacer from Arabidopsis thaliana. (semanticscholar.org)
  • Structural and transcriptional characterization of the external spacer of a ribosomal RNA nuclear gene from a higher plant. (semanticscholar.org)
  • At all salt concentrations studied, however, the length distribution of spacer and gene regions was identical. (uni-wuerzburg.de)
  • The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. (pnas.org)
  • The nuclear ribosomal small subunit has poor species-level resolution in fungi. (pnas.org)
  • SSU rDNA - small subunit ribosomal DNA. (scribd.com)
  • The multigene family encoding the 5S rRNA, one of the most important structurally-functional part of the large ribosomal subunit, is an obligate component of all eukaryotic genomes. (biomedcentral.com)
  • Ultrastructure and small-subunit ribosomal DNA sequence of Henneguya lesteri n.sp. (int-res.com)
  • The currently accepted 25 species of the genus Trichosporon were examined as shown in Table 1 Trichosporon DNA was extracted by the method of Makimura et al. (asm.org)
  • Ribosomal DNA (rDNA) of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer (IGS). (mdpi.com)
  • However, recent research utilizing different mitochondrial and nuclear DNA markers has allowed researchers to begin to reassess zoanthid species identification [ 9 , 10 ]. (hindawi.com)
  • Species-specific repeat units in the intergenic spacer of the ribosomal RNA cistron of Anopheles aquasalis Curry. (ajtmh.org)
  • Here, male and female genitalia, DNA sequences, and the effects of within- and between-species pairings on egg production and egg development were examined to develop a better understanding of the relationship between these two species. (bioone.org)
  • Molecular barcodes, typically one or several small stretches of DNA, provide valuable characters to delineate species in all eukaryotic kingdoms of life. (royalsocietypublishing.org)
  • This work aimed to optimize an efficient and simple protocol for DNA extraction of Camarotella species, an obligate plant pathogen that cause verrucosis or "lixa" on coconut tree and other palms, facilitating the molecular studies of these biotrophic microorganisms. (scielo.br)
  • The method proposed enabled a fast, reproducible and reliable DNA extraction from Camarotella species. (scielo.br)
  • The use of DNA molecular techniques has been largely applied for species characterization. (scielo.br)
  • Analysis of DNA sequences from the ITS region indicated that populations from Costa Rica were monophyletic with S. bradys from West Africa and clearly distinct from other Scutellonema species. (brill.com)
  • Discrete differences in DNA sequence were identified between strains of P. carduorum, allowing us to discriminate between those specific to individual Carduus host species. (usda.gov)
  • In this research, a set of ISSR (Inter-Simple Sequence Repeat polymorphic DNA) and SSR (Simple Sequence Repeat polymorphic DNA) molecular markers were standardized to characterize S. capitata and S. macrocephala species and evaluate the genetic purity of commercial samples. (scielo.br)
  • Analysis of the distribution of nucleotide variation suggested that the populations were quite divergent, a result that is concordant with previous surveys of allozyme and mitochondrial DNA variation. (genetics.org)
  • We utilize DNA sequence data from the nuclear, chloroplast, and mitochondrial genomes to infer historical processes of biodiversification. (duke.edu)
  • The methodology provided PCR-quality mitochondrial and nuclear DNA from laboratory cercariae, miracidia and eggs that had been stored at up to 37°C for 2 weeks and at 4°C for 6 months and also from field collected samples. (biomedcentral.com)
  • Interspecific and intrapopulation variation in mitochondrial ribosomal DNA sequences of Mytilus spp. (nii.ac.jp)
  • [12] This conclusion has been supported not only by the degree of interrelation shown by different groups within the family compared with that found among the Leguminosae and their closest relations, but also by all the recent phylogenetic studies based on DNA sequences. (eol.org)
  • The unresolved monophyly of Exbucklandioideae and the clades of Disanthoideae, Rhodoleioideae, Exbucklandioideae or even Mytilarioideae being a sister clade to Hamamelidoideae may have been a result of differing DNA methodologies researchers have used to produce phylogenetic trees and the inclusion or exclusion of certain genera used as outgroups in their analyses. (eol.org)
  • An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence requirements. (asm.org)
  • Identification of Serratia marcescens on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. (docphin.com)
  • Chloroplasts of the unicellular flagellate eukaryote Euglena gracilis contain several copies of a circular 135-140-kilobase pair DNA 1 which codes for chloroplast-specific stable RNAs (16S, 23S (refs 2, 3), 5S rRNAs 4 and tRNAs 5 ) and for an unknown number of chloroplast-specific proteins. (nature.com)
  • Total chloroplast tRNA hybridizes to fragments of rDNA 9 and it was suggested that the 16S-23S spacer region contains tRNA coding sequences as is observed in Escherichia coli 10,11 and in spinach chloroplast 12 rDNA. (nature.com)
  • Sequence analysis of a total spacer in E. gracilis should demonstrate whether such similarities are also preserved in the chloroplast rDNA spacer region, or if this region has suffered a higher genetic drift rate. (nature.com)
  • Meiotic Stability, Chloroplast DNA Polymorphisms, and Morphological Traits of Upland x Lowland Switchgrass Reciprocal Hybrids. (thefreelibrary.com)
  • Both A. aquasalis-specific clones were from the intergenic spacer region of the ribosomal RNA (rRNA) cistron. (ajtmh.org)
  • The DNA sequence of a highly conserved region of these repeats was used to synthesize an oligonucleotide probe specific to A. aquasalis. (ajtmh.org)
  • The repeating unit of 5S rDNA contains a 120 bp coding region and a non-transcribed spacer sequence (NTS). (biomedcentral.com)
  • To give a common reference structure, an outline corresponding to the nucleolar region labelled by a probe to the external transcribed spacer (ETS) portion of the pre‐rRNA is shown. (els.net)
  • The intergenic spacer region of the rDNA in Haplopappus gracilis (Nutt. (semanticscholar.org)
  • The intergenic spacer region of the rDNA in Olea europaea L. (semanticscholar.org)
  • The rDNA intergenic spacer region nucleotide sequence of Hordeum bulbosum L. (semanticscholar.org)
  • Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheat. (semanticscholar.org)
  • A fingerprint of the total bacterial community was assessed by ribosomal intergenic spacer region analysis (RISA). (asm.org)
  • A SYBR Green real-time PCR targeting the conserved region of kinetoplast DNA minicircles was able to differentiate between Leishmania subgenera. (biomedcentral.com)
  • Therefore, the analysis of thermal dissociation curves targeting the conserved kinetoplast DNA minicircles region is able to provide a rapid and reliable method to identify the main etiologic agents of cutaneous and visceral leishmaniases in endemic regions of Brazil. (biomedcentral.com)
  • RNA was reverse-transcribed into cDNA, and together with the extracted total DNA, a region of the ITS1 (230−422 bp) of DNA and cDNA using a nested PCR approach was amplified for Illumina Miseq sequencing. (ncl.edu.tw)
  • Ribosomal DNA evolution at the population level: nucleotide variation in intergenic spacer arrays of Daphnia pulex. (genetics.org)
  • Nucleotide variation was surveyed in 21 subrepeat arrays from the ribosomal DNA intergenic spacer of three Daphnia pulex populations. (genetics.org)
  • We report here two highly reliable ribosomal DNA-based single nucleotide polymorphism (SNP) markers, CxpG2T and CxpA2d, for detecting Cx. (ajtmh.org)
  • When the spacer amplification products were resolved by electrophoresis, the resulting patterns could be used to distinguish all of the strains tested into 3 groups (group A-35, group B-3 and group C-2 isolates). (docphin.com)
  • A timing study of DNA amplification in Xenopus laevis oocytes. (xenbase.org)
  • Observations on early germ cell development and premeiotic ribosomal DNA amplification in Xenopus laevis. (xenbase.org)
  • Transcriptional silencing in Saccharomyces cerevisiae occurs at several genetic loci, including the ribosomal DNA (rDNA). (asm.org)
  • PCR primers were engineered from the DNA sequence of a RAMS amplicon generated by PCR from the P. jaceae strain. (usda.gov)
  • Ribosomal DNA of one individual from Assam state did not amplify in the ASPCR, possessed 786 base pair long ITS2 sequence and showed 99.7% similarity with the sequence of An. (nih.gov)
  • The shortest two IGS types, A and B, lacked the transcription initiation site, non-transcribed spacer, and external transcribed spacer. (mdpi.com)
  • The other four IGSs had subtle variations in the transcription termination site, guanine-cytosine (GC) content, and number of tandem repeats, but the external transcribed spacers of these four IGSs were quite similar in length. (mdpi.com)
  • Six DNA regions were evaluated as potential DNA barcodes for Fungi , the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. (pnas.org)
  • Sequence comparisons also revealed a great similarity between the CHO and mouse spacer promoter regions, yet there was much less similarity between the flanking sequences. (asm.org)
  • Our comparison of methods suggests that without previous knowledge of the DNA sequence of the IGS and flanking regions, both methods be used to accurately measure IGS length. (biomedcentral.com)
  • To achieve these high levels of ribosome production, eukaryotes have multiple copies of ribosomal (r)DNA, arranged in tandem in the Nucleolus Organizer Regions (NORs) of one or more chromosomes. (biomedcentral.com)
  • The observations indicate that (i) rDNA spacer regions are not compacted into nucleosomal particles and into supranucleosomal structures when visualized at chromatin stabilizing salt concentrations (e.g., 20 mM NaCl), and (ii) spacer DNA is covered by a uniform layer of proteins of unknown nature which, at very low salt concentrations (below 1 mM NaCl), can artificially give rise to the appearance of small granular particles of approximately nucleosome-like sizes. (uni-wuerzburg.de)
  • Ribotyping is generally considered the best method, but it fails to account for sequence diversity which might exist in intergenic 16S-23S rRNA spacer regions (ISRs) within and among strains of this organism. (asm.org)
  • We conclude that the intergenic 16S-23S rRNA spacer regions showed a high degree of diversity, not only among the rrn operons in different strains and different rrn copies in a single strain but also among ISRs of the same length. (asm.org)
  • Miller spread of ribosomal DNA (rDNA) transcription units (Christmas trees), redrawn from an original micrograph. (els.net)
  • The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. (asm.org)
  • Analysis of the DNA sequence of this fragment revealed four tandemly repeated 36-bp units. (ajtmh.org)
  • The aim of this study was to develop, refine and evaluate an alternative methodology, which enables better preservation of large numbers of individual schistosome larval stages and eggs collected in low resource endemic areas, to provide PCR-quality DNA for multi-locus genetic analysis. (biomedcentral.com)
  • Ribosomal RNA (rRNA) intergenic spacer analysis (RISA) is a method of microbial community analysis that provides a means of comparing differing environments or treatment impacts without the bias imposed by culture- dependent approaches. (wikipedia.org)
  • A simplified DNA extraction method for PCR analysis of Camarotella spp. (scielo.br)
  • Evolutionary considerations argue strongly that the presence of ribosomal DNA spacer promoters offers a significant selective advantage. (asm.org)
  • Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of Brassica oleracea L. var. (mdpi.com)
  • However, this knowledge gap, caused by the lack of diagnostic morphological features in small organisms and the limited sampling of the global ocean, is currently being filled, thanks to new DNA-based approaches. (royalsocietypublishing.org)
  • The latter is suggested from the 189 bases which have been sequenced from the 2.4-kilobase pair rDNA spacer from maize chloroplasts 14 . (nature.com)
  • Bou Samra E, Buhagiar-Labarchède G, Machon C, Guitton J, Onclercq-Delic R, Green MR, Alibert O, Gazin C, Veaute X, Amor-Guéret M. A role for Tau protein in maintaining ribosomal DNA stability and cytidine deaminase-deficient cell survival. (umassmed.edu)
  • Sontheimer EJ, Marraffini LA. Microbiology: slicer for DNA. (umassmed.edu)
  • 1991). The main disadvantage of these methods is the need to cultivate the fungus to obtain the mycelium, making difficult the DNA extraction for obligate parasites. (scielo.br)