DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
The intergenic DNA segments that are between the ribosomal RNA genes (internal transcribed spacers) and between the tandemly repeated units of rDNA (external transcribed spacers and nontranscribed spacers).
Any of the DNA in between gene-coding DNA, including untranslated regions, 5' and 3' flanking regions, INTRONS, non-functional pseudogenes, and non-functional repetitive sequences. This DNA may or may not encode regulatory functions.
Constituent of 50S subunit of prokaryotic ribosomes containing about 3200 nucleotides. 23S rRNA is involved in the initiation of polypeptide synthesis.
Constituent of the 60S subunit of eukaryotic ribosomes. 5.8S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The relationships of groups of organisms as reflected by their genetic makeup.
Devices that cause a liquid or solid to be converted into an aerosol (spray) or a vapor. It is used in drug administration by inhalation, humidification of ambient air, and in certain analytical instruments.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
A small aerosol canister used to release a calibrated amount of medication for inhalation.
Repetitive nucleic acid sequences that are principal components of the archaeal and bacterial CRISPR-CAS SYSTEMS, which function as adaptive antiviral defense systems.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Procedures for identifying types and strains of fungi.
Infections resulting from the implantation of prosthetic devices. The infections may be acquired from intraoperative contamination (early) or hematogenously acquired from other sites (late).
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.
A short-acting beta-2 adrenergic agonist that is primarily used as a bronchodilator agent to treat ASTHMA. Albuterol is prepared as a racemic mixture of R(-) and S(+) stereoisomers. The stereospecific preparation of R(-) isomer of albuterol is referred to as levalbuterol.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Constituent of the 40S subunit of eukaryotic ribosomes. 18S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
A series of hydrocarbons containing both chlorine and fluorine. These have been used as refrigerants, blowing agents, cleaning fluids, solvents, and as fire extinguishing agents. They have been shown to cause stratospheric ozone depletion and have been banned for many uses.
Adhesives used to fix prosthetic devices to bones and to cement bone to bone in difficult fractures. Synthetic resins are commonly used as cements. A mixture of monocalcium phosphate, monohydrate, alpha-tricalcium phosphate, and calcium carbonate with a sodium phosphate solution is also a useful bone paste.
The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.
Agents that cause an increase in the expansion of a bronchus or bronchial tubes.
Constituent of the 60S subunit of eukaryotic ribosomes. 28S rRNA is involved in the initiation of polypeptide synthesis in eukaryotes.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Genotypic differences observed among individuals in a population.
Copies of nucleic acid sequence that are arranged in opposing orientation. They may lie adjacent to each other (tandem) or be separated by some sequence that is not part of the repeat (hyphenated). They may be true palindromic repeats, i.e. read the same backwards as forward, or complementary which reads as the base complement in the opposite orientation. Complementary inverted repeats have the potential to form hairpin loop or stem-loop structures which results in cruciform structures (such as CRUCIFORM DNA) when the complementary inverted repeats occur in double stranded regions.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Compressed gases or vapors in a container which, upon release of pressure and expansion through a valve, carry another substance from the container. They are used for cosmetics, household cleaners, and so on. Examples are BUTANES; CARBON DIOXIDE; FLUOROCARBONS; NITROGEN; and PROPANE. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Genetic loci which direct transcription of ribosomal RNA in bacterial operons. They are designated rrnB, rrnC, rrnD, etc. according to the structural position of the transcription unit in the DNA sequence.
A transfer RNA which is specific for carrying alanine to sites on the ribosomes in preparation for protein synthesis.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
Deoxyribonucleic acid that makes up the genetic material of CHLOROPLASTS.
Constituent of the 50S subunit of prokaryotic ribosomes containing about 120 nucleotides and 34 proteins. It is also a constituent of the 60S subunit of eukaryotic ribosomes. 5S rRNA is involved in initiation of polypeptide synthesis.
Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
An anti-inflammatory, synthetic glucocorticoid. It is used topically as an anti-inflammatory agent and in aerosol form for the treatment of ASTHMA.
A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.

Utility of internally transcribed 16S-23S rDNA spacer regions for the definition of Pseudomonas stutzeri genomovars and other Pseudomonas species. (1/1629)

Bacteria identified and classified as Pseudomonas stutzeri, on the basis of traditional criteria, are recognized to be markedly heterogeneous, such that a systematic phenotypic characterization has not been correlated with genotypic groupings (i.e. genomovars) based upon DNA-DNA similarities. The internally transcribed 16S-23S rDNA spacer (ITS1) regions of P. stutzeri were analysed with respect to the ability of these nucleic acid regions to differentiate and identify the genomic groups (i.e. genomovars) of P. stutzeri. The ITS1s of 34 strains of P. stutzeri were amplified by PCR and the PCR product was subjected to RFLP analysis, which allowed the differentiation and identification of the strains to their respective genomovars. Sequence determination and analysis of ITS1s supported further the results obtained by RFLP, i.e. nucleotide signatures were identified in strains belonging to different genomovars. The ITS1s of all strains of P. stutzeri contained the tandem tRNA(Ile)/tRNA(Ala) genes and did not exhibit distinct sequence heterogeneity between different operons of a strain. Phylogenetically informative variable sites were located, exclusively, in non-coding regions. The results of the RFLP and sequence analysis of ITS1s supported and correlated with the phylogenetic relationships estimated from 16S rRNA gene sequence comparisons and DNA-DNA hybridizations, offering an alternative tool for genomovar and species differentiation.  (+info)

High intraindividual variation in internal transcibed spacer sequences in Aeschynanthus (Gesneriaceae): implications for phylogenetics. (2/1629)

Aeschynanthus (Gesneriaceae) is a large genus of tropical epiphytes that is widely distributed from the Himalayas and China throughout South-East Asia to New Guinea and the Solomon Islands. Polymerase chain reaction (PCR) consensus sequences of the internal transcribed spacers (ITS) of Aeschynanthus nuclear ribosomal DNA showed sequence polymorphism that was difficult to interpret. Cloning individual sequences from the PCR product generated a phylogenetic tree of 23 Aeschynanthus species (two clones per species). The intraindividual clone pairs varied from 0 to 5.01%. We suggest that the high intraindividual sequence variation results from low molecular drive in the ITS of Aeschynanthus. However, this study shows that, despite the variation found within some individuals, it is still possible to use these data to reconstruct phylogenetic relationships of the species, suggesting that clone variation, although persistent, does not pre-date the divergence of Aeschynanthus species. The Aeschynanthus analysis revealed two major clades with different but overlapping geographic distributions and reflected classification based on morphology (particularly seed hair type).  (+info)

High-resolution phylogenetic analysis of NO2--oxidizing Nitrobacter species using the rrs-rrl IGS sequence and rrl genes. (3/1629)

A high-resolution phylogenetic analysis of Nitrobacter strains and their neighbours was made using the rrs-rrl intergenic spacer sequence and the hypervariable part of the rrl gene. The phylogenetic tree obtained was consistent with that which was obtained previously but was much more discriminating, permitting the design of genus-specific primers.  (+info)

Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae. (4/1629)

On the basis of genetic analysis, molecular karyotyping and sequence analyses of the 18S rRNA and internal transcribed spacer (ITS) region, three new Saccharomyces species are described, Saccharomyces cariocanus (with type strain NCYC 2890T), Saccharomyces kudriavzevii (with type strain NCYC 2889T) and Saccharomyces mikatae (with type strain NCYC 2888T). Genetic and molecular analyses did not confirm the previously observed conspecificity of Saccharomyces paradoxus and S. cariocanus. The latter species exhibits postzygotic isolation from representative strains from all known geographical populations of S. paradoxus: European, Far-East Asian, North American and Hawaiian.  (+info)

Molecular systematics of European Hyalodaphnia: the role of contemporary hybridization in ancient species. (5/1629)

We examined phylogenetic relationships among Daphnia using mitochondrial DNA (mtDNA) sequences from the small subunit ribosomal RNA (12S), cytochrome c oxidase subunit I and nuclear DNA sequences from the first and second internal transcribed spacer representing 1612 base positions. Phylogenetic analyses using several species of the three main Daphnia subgenera, Ctenodaphnia, Hyalodaphnia and Daphnia, revealed that the Hyalodaphnia are a monophyletic sister group of the Daphnia. Most Hyalodaphnia species occur on one continent, whereas only three are found in North America and Europe. Endemicity of species is associated with variation in thermal tolerance and habitat differentiation. Although many species of the Hyalodaphnia are known to hybridize in nature, mtDNA divergence is relatively high ca. 9%) compared to other hybridizing arthropods (ca. 3%). Reproductive isolation in Daphnia seems to evolve significantly slower than genetic isolation. We related these findings to what is known about the ecology and genetics of Daphnia in order to better understand the evolutionary diversification of lineages. The relationship of these data to phylogenetic patterns is discussed in the context of speciation processes in Daphnia.  (+info)

Detection and identification of mycobacteria by amplification of the internal transcribed spacer regions with genus- and species-specific PCR primers. (6/1629)

We evaluated the usefulness of PCR assays that target the internal transcribed spacer (ITS) region for identifying mycobacteria at the species level. The conservative and species-specific ITS sequences of 33 species of mycobacteria were analyzed in a multialignment analysis. One pair of panmycobacterial primers and seven pairs of mycobacterial species-specific primers were designed. All PCRs were performed under the same conditions. The specificities of the primers were tested with type strains of 20 mycobacterial species from the American Type Culture Collection; 205 clinical isolates of mycobacteria, including 118 Mycobacterium tuberculosis isolates and 87 isolates of nontuberculous mycobacteria from 10 species; and 76 clinical isolates of 28 nonmycobacterial pathogenic bacterial species. PCR with the panmycobacterial primers amplified fragments of approximately 270 to 400 bp in all mycobacteria. PCR with the M. tuberculosis complex-specific primers amplified an approximately 120-bp fragment only for the M. tuberculosis complex. Multiplex PCR with the panmycobacterial primers and the M. tuberculosis complex-specific primers amplified two fragments that were specific for all mycobacteria and the M. tuberculosis complex, respectively. PCR with M. avium complex-, M. fortuitum-, M. chelonae-, M. gordonae-, M. scrofulaceum-, and M. szulgai-specific primers amplified specific fragments only for the respective target organisms. These novel primers can be used to detect and identify mycobacteria simultaneously under the same PCR conditions. Furthermore, this protocol facilitates early and accurate diagnosis of mycobacteriosis.  (+info)

Divergent mechanisms of 5' 23S rRNA IVS processing in the alpha-proteobacteria. (7/1629)

Widespread occurrence of a separate small RNA derived from the 5'-end of 23S rRNA and of an intervening sequence (IVS) which separates this domain from the main segment of 23S rRNA in the alpha-proteobacteria implies that processing reactions which act to excise the IVS are also maintained in this group. We previously characterized the first example of processing of this IVS in Rhodopseudomonas palustris, which is classified with the Bradyrhizobia In this case, IVS excision occurs by a multistep process and RNase III appears to act at an early step. Here, we characterize in vivo and in vitro IVS processing in two other related, but phenotypically distinct, Bradyrhizobia We also examine in vivo and in vitro processing of rRNA precursors from a more distantly related alpha-proteobacterium, Rhodobacter sphaeroides which produces a separate 5' 23S rRNA domain but has different sequences in the 5' 23S rRNA IVS. The details of the in vivo processing of all of the Bradyrhizobial rRNAs closely resemble the R. palustris example and in vitro studies suggest that all of the Bradyrhizobia utilize RNase III in the first step of IVS cleavage. Remarkably, in vivo and in vitro studies with R.sphaeroides indicate that initial IVS cleavage uses a different mechanism. While the mechanism of IVS cleavage differs among these alpha-proteobacteria, in all of these cases the limits of the internal segments processed in vivo are almost identical and occur far beyond the initial cleavage sites within the IVSs. We propose that these bacteria possess common secondary maturation pathways which enable them to generate similarly processed 23S rRNA 5'- and 3'-ends.  (+info)

Strain identification of Trichophyton rubrum by specific amplification of subrepeat elements in the ribosomal DNA nontranscribed spacer. (8/1629)

Trichophyton rubrum is the commonest cause of dermatophytosis of skin and nail tissue. Molecular characterization of the T. rubrum ribosomal DNA nontranscribed-spacer region revealed two novel tandemly repetitive subelements (TRSs): TRS-1, containing a 27-bp palindromic sequence, and TRS-2. Specific amplification of TRS-1 produced strain-characteristic banding patterns (PCR types), with 21 TRS-1 PCR types recognized from 101 clinical isolates. Four simple patterns representing 1 to 4 copies of TRS-1 accounted for 75 (75%) of all 101 strains, whereas more complex patterns were observed for 21 (20%) of the 101 isolates. The copy number of TRS-2 was 0 to 3 repeats per cistron, with a majority of isolates having two copies of this element. Eleven isolates were polymorphic for TRS-2, and in combination, 23 separate PCR types were recognized by amplification of both TRS-1 and TRS-2. The PCR patterns from both elements were stable and reproducible. Elements with homology to TRS-1 were present in three phylogenetically related species, Trichophyton violaceum, Trichophyton gourvilii, and Trichophyton soudanense, but these elements were not identified in other dermatophyte taxa. There was no clear correlation of PCR type with specimen (skin or nail tissue), but certain PCR types appeared to show a bias in geographic distribution. This new method of typing T. rubrum will enable important questions about pathogenesis and epidemiology of this fungus to be addressed.  (+info)

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Chen, H.,Lim, C.K.,Lee, Y.K.,Chan, Y.N. (2000). Comparative analysis of the genes encoding 23S-5S rRNA intergenic spacer regions of Lactobacillus casei-related strains. International Journal of Systematic and Evolutionary Microbiology 50 (2) : 471-478. [email protected] Repository ...
The 2,030 isolates analyzed in the present study comprised 1,235 isolates from stool samples from hospital patients, 395 isolates from stool samples referred via community practitioners, 150 isolates from the hospital environment, 27 isolates from veterinary sources, 89 isolates from the general environment, 1 isolate from an extraintestinal human site, and 133 reference strains held in the National Collection of Type Cultures, the American Type Culture Collection and the Culture Collection, University of Göteborg, and in the personal collections of C. difficile types held by Delmee and others (6, 16) and other members of the International Study Group on C. difficile (3).. PCR ribotyping was performed in duplicate, with slight modifications to a method described previously (13). Briefly, bacteria were harvested from overnight anaerobic cultures on Fastidious Anaerobe Agar (LabM, Bury, United Kingdom) supplemented with 6% horse blood. Crude template nucleic acid was prepared by resuspension of ...
Staphylococcus aureus is an important human and animal pathogen, and is regarded as an important cause of intramammary infection (IMI) in ruminants. Staphylococcus aureus genetic variability and virulence factors have been well studied in veterinary medicine, especially in cows as support for control and management of IMI. The aim of the present study was to genotype 71 Staph. aureus isolates from the bulk tank and foremilk of water buffaloes (n. = 40) and from udder tissue (n. = 7) and foremilk (n. = 24) from small ruminants. The method used was previously applied to bovine Staph. aureus and is based on the amplification of the 16S-23S rRNA intergenic spacer region. The technique applied was able to identify different Staph. aureus genotypes isolated from dairy species other than the bovine species, and cluster the genotypes according to species and herds. Virulence gene distribution was consistent with genotype differentiation. The isolates were also characterized through determination of the ...
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The University of Auckland Library This study describes the extent of variability in biofilm bacterial community structure across a broad range of spatial and temporal scales and assesses whether this may be used as an indicator of stream ecological health and function. A community DNA fingerprinting technique (Automated Ribosomal Intergenic Spacer Analysis - ARISA) was used to examine the structure of bacterial communities within freshwater stream biofilms. When compared with macrobenthic invertebrate community assemblages using multi-dimensional scaling techniques, similar broad-scale trends in population structure were revealed between organisms at these different trophic levels. For both communities, spatial variability in community structure was greater between streams than within each site, or compared to temporal variability measured over 1 year. Distance-based redundancy analysis of both bacterial ARISA and macroinvertebrate data estimated that the largest cause of variation in community ...
Freshwater streams display both temporal (time) and spatial (space) differences. Time variation is a result of seasonal influences and space variation is due to flow regime, substrate type, water solutes, suspended materials and incident light exposure. It is believed that bacterial communities are good indicators due to their rapid life cycle. However, if we cant see them, how do we know that they are present and observe changes? Bacteria can be detected using Automated Ribosomal Intergenic Spacer Analysis (ARISA) which creates fingerprints of microbial communities. The fingerprint produced is just like a human fingerprint, because it is unique to a bacteria species just like a fingerprint is unique to a person ...
The Crystal Refinery is a former refinery site in Carson City Michigan that has had historic releases of refined and unrefined crude oil dating back to the late 1940s. The contamination of soil with light non-aqueous phase liquids (LNAPL) has had a dynamic impact on the subsurface environment in adjacent Carson City Park. Very little is known about the complex interactions between microbial communities, geochemistry and geophysics. In order to investigate possible connections between these parameters a multidisciplinary study was undertaken to investigate the hypothesis that the degradation of LNAPL by resident microbial communities causes a local increase in organic acid concentrations, which in turn cause an increase in native mineral weathering and a concurrent decrease in the bulk electrical resistivity of soil.Microbial community structure was analyzed using a 96-well most probable number (MPN) method and rDNA intergenic spacer region analysis (RISA). In addition to the observational study of this
An apparatus for killing insects in produce, which comprises an outer container; means for dividing a space enclosed by the outer container into first and second internal chambers; means for supporting a plurality of bulk produce bins in the first internal chamber; first and second air passage means for selectively allowing passage of air between the first internal chamber and the second internal chamber; means for circulating air in the second internal chamber successively through one of the air passage means, the first internal chamber, the other of the air passage means, and back into the second internal chamber; and means for heating the circulating air.
A trial spacer kit has a plurality of trial spacers of varying heights. Each spacer includes a disc having a top surface and a bottom surface defining a height of said spacer, an annular groove formed in said disc that defines a central trunk of said spacer, the central trunk extending between the top and bottom surfaces of said spacer, a first radial flange provided at an upper end of the central trunk and a second radial flange provided at a lower end of the central trunk. The annular grooves of the spacers have a common dimension. The radial flanges of a first one of the spacers is thicker than the radial flanges of a second one of the spacers so that the first one of the spacers has a greater height than the second one of the spacers.
Under the intluence of 5-tluoro-uridine, the ultrastructure of the rDNA transcription units in Xenopus oocytes is altered. Whereas part of the matrix units maintains anormal aspect or shows various degrees of inhibition, in a strong proportion of the transcription units the alternating pattern of matrix units and fibril-free spacer regions is no longer recognized. Transcriptional complexes are found along the entire DNP axis, including the regions of the spacers. These observations support biochemical data on transcription in rDNA spacer region ...
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Lactobacillus acetotolerans strain ATCC 43578 16S ribosomal RNA gene,partial sequence; 16S-23S intergenic spacer, complete sequence; and 23Sribosomal RNA gene, partial ...
關於生長在藻類上的真菌研究缺乏,特別是海洋大型藻類,而前人只針對小部份褐藻、綠藻和紅藻進行初部研究。因此本研究選取臺灣北部的優勢紅藻細翼枝菜 (Pterocladiella capillacea) 作為研究對象,以培養法分離真菌及非培養法 (metabarcoding) 結果比較細翼枝菜上的真菌多樣性。於培養法中,將洗淨的健康及死亡藻體置於葡萄糖-酵母萃取物-蛋白腖瓊脂海水培養基 (GYPS) 及人工海水瓊脂培養基 (SWA) 上,待菌絲從藻體生長出來後便繼代於玉米粉瓊脂培養基 (CMAS) 上,依其菌落形態分群。利用聚合酶連鎖反應 (polymerase chain reaction, PCR) 擴增其核醣體基因 (rDNA) 內轉錄間隔區 (internal transcribed spacer, ITS) 及核醣體大亞基 (large subunit, LSU) 並進行測序,最後與NCBI的GenBank資料庫序列進行比較以鑑種。於高通量定序中,從冷凍乾燥後的細翼枝菜藻體萃取總DNA和RNA
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Ribosomal DNA (rDNA) of plants is present in high copy number and shows variation between and within species in the length of the intergenic spacer (IGS). The 45S rDNA of flowering plants includes the 5.8S, 18S and 25S rDNA genes, the internal transcribed spacer (ITS1 and ITS2), and the intergenic spacer 45S-IGS (25S-18S). This study identified six different types of 45S-IGS, A to F, which at 363 bp, 1121 bp, 1717 bp, 1969 bp, 2036 bp and 2111 bp in length, respectively, were much shorter than the reported reference IGS sequences in B. oleracea var. alboglabra. The shortest two IGS types, A and B, lacked the transcription initiation site, non-transcribed spacer, and external transcribed spacer. Functional behavior of those two IGS types in relation to rRNA synthesis is a subject of further investigation. The other four IGSs had subtle variations in the transcription termination site, guanine-cytosine (GC) content, and number of tandem repeats, but the external transcribed spacers of these four IGSs were
While the 16S ribosomal gene is the standard for distinguishing among bacterial species, it is insufficiently variable among P. gingivalis strains for resolution. The ribosomal ISR is the most variable portion of the ribosomal operon and is sufficiently variable within a species to resolve strains (18). This locus also offers a technical advantage since it can be amplified with species-specific and conserved primers in the 16S and 23S ribosomal genes, thus avoiding the need to culture bacteria from clinical specimens. There are four copies of the ribosomal operon in P. gingivalis (P. gingivalis genome project [http://www.forsyth.org/pggp/number_of_rrna_operons.htm]). Ribosomal operons in general, including the ISR, are maintained by concerted evolution (2, 4). This correction minimizes replacement by exogenous sequences, so that the operon should closely reflect the evolutionary history of the organism.. We have previously used the ISR for both sequence-based and heteroduplex type-based P. ...
Author contributions: C.L.S. and K.A.S. designed research; K.A.S., V.R., E.B., K.V., P.W.C., A.N.M., M.J.W., M.C.A., K.-D.A., F.-Y.B., R.W.B., D.B., M.-J.B., M. Blackwell, T.B., M. Bogale, N.B., A.R.B., B.B., L.C., Q.C., G.C., P. Chaverri, B.J.C., A.C., P. Cubas, C.C., U.D., Z.W.d.B., G.S.d.H., R.D.-P., B. Dentinger, J.D-U., P.K.D., B. Douglas, M.D., T.A.D., U.E., J.E.E., M.S.E., K.F., M.F., M.A.G., Z.-W.G., G.W.G., K.G., J.Z.G., M. Groenewald, M. Grube, M. Gryzenhout, L.-D.G., F. Hagen, S. Hambleton, R.C.H., K. Hansen, P.H., G.H., C.H., K. Hirayama, Y.H., H.-M.H., K. Hoffmann, V. Hofstetter, F. Högnabba, P.M.H., S.-B.H., K. Hosaka, J.H., K. Hughes, Huhtinen, K.D.H., T.J., E.M.J., J.E.J., P.R.J., E.B.G.J., L.J.K., P.M.K., D.G.K., U.K., G.M.K., C.P.K., S.L., S.D.L., A.S.L., K.L., L.L., J.J.L., H.T.L., H.M., S.S.N.M., M.P.M., T.W.M., A.R.M., A.S.M., W.M., J.-M.M., S.M., L.G.N., R.H.N., T.N., I.N., G.O., I. Okane, I. Olariaga, J.O., T. Papp, D.P., T. Petkovits, R.P.-B., W.Q., H.A.R., D.R., T.L.R., ...
Respiratory infection in cystic fibrosis (CF) is polymicrobial, but standard sputum microbiology does not account for the lung microbiome or detect changes in microbial diversity associated with disease. As a clinically applicable CF microbiome surveillance scheme, total sputum nucleic acids isolated by a standard high-throughput robotic method for accredited viral diagnosis were profiled for bacterial diversity using ribosomal intergenic spacer analysis (RISA) PCR. Conventional culture and RISA were performed on 200 paired sputum samples from 93 CF adults; pyrosequencing of the 16S rRNA gene was applied to 59 patients to systematically determine bacterial diversity. Compared to the microbiology data, RISA profiles clustered into two groups: the emerging nonfermenting Gram-negative organisms (eNFGN) and Pseudomonas groups. Patients who were culture positive for Burkholderia, Achromobacter, Stenotrophomonas, and Ralstonia clustered within the eNFGN group. Pseudomonas group RISA profiles were ...
In recent years, DNA barcoding is considered as a universal species identification method for plants. It mainly involves discrimination of species through standardized molecular marker gene and is gaining support from the taxonomists as well. DNA barcoding has wider applications in different studies namely to predict cryptic species, to study biological samples in forensics and conservation sciences for characterization of biodiversity; to track inventory for plants identity or purity and in ecological species diversity studies [1-4]. Various molecular markers have been used for DNA barcoding studies. A 650 base pair (bp) of the mitochondrial cytochrome c oxidase unit I (COI) gene was used as a barcode in various organisms such as animals, birds, fishes, insects and nematodes [4-8]. A specific region of the nuclear ribosomal internal transcribed spacer (ITS) gene is the well-studied DNA barcode for fungi [9]. In plant DNA barcoding, there has been extensive debate about the locus choice; several ...
TY - JOUR. T1 - Report of the second internal workshop on human chromosome 5 mapping. AU - Westbrook, C. A.. AU - Neuman, W. L.. AU - Mcpherson, John Douglas. AU - Camper, S.. AU - Wasmuth, J.. AU - Plaetke, R.. AU - Williamson, R.. PY - 1992. Y1 - 1992. UR - http://www.scopus.com/inward/record.url?scp=0026677059&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026677059&partnerID=8YFLogxK. M3 - Article. C2 - 1362532. AN - SCOPUS:0026677059. VL - 61. SP - 226. EP - 231. JO - Cytogenetic and Genome Research. JF - Cytogenetic and Genome Research. SN - 1424-8581. IS - 4. ER - ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
the spacers around your I am guessing if your son has perfect spacers for your Using your Spacer Eibach PRO-SPACER, FERRARI 360 teeth enhance your teeth side cone spacer and your SPACERS FOR TEETH - Page 5 what are spacers for your Keep his teeth andental health spacers for teeth, google Spacers For Your Teeth yahoo Spacers For Your Teeth mages images ...
Quality means everything in any Toyota bZ4X hub wheel spacers. While you may consider that a simple and cheap wheel spacer is just enough to use, the fact is, lower quality means lower safety. Never skimp on price and end up with unsafe low-quality spacers. The quality is always a huge deal when it comes to the components you add to your Toyota bZ4X. In the market, wheel spacers made from aircraft-grade aluminum are the basis of quality fender spacers. When you find a reputable store, youll find that they use 6061-T6 or 7075-T6 billet aluminum as the material of spacers. This ensures strength, service range, and safety as well as high resistance to corrosion. If the store youre buying from cant confidently say that it uses aircraft-grade 6061-T6 or 7075-T6 billet aluminum, you should probably find the nearest exit. Although, many stores claim that they are selling 6061-T6 aluminum spacers, but they are not made by the forged process. In fact, they are cheap casting spacers. Forging provides ...
Spacer 9 is a triethylene glycol chain that is 9 atoms long (6 carbons + 3 oxygens), and is used to incorporate a spacer arm into an oligonucleotide. Spacer 9 can be incorporated in consecutive additions whenever a longer spacer is required. Spacer 9 has been used to form non-nucleotide bridges in hairpin loops in oligonucleotides (1), for linking oligonucleotides to epitopes for drug development (2), and for solid-phase immobilization of hybridization probes (3). Multiple incorporation of Spacer 9 has been used to form long, flexible linker arms between the two domains (double-helix forming and triple-helix forming, respectively) of a bifunctional DNA oligonucleotide, in order to maximize the binding flexibility of the two domains for their respective targets (4). This oligo was used to form a peptide nucleic acid (PNA)-DNA conjugate for use in site-directed recombination applications.. References ...
We explored the potential of the cox1 gene in the species resolution of soil fungi and compared it with the nuclear internal transcribed spacer (ITS) and small subunit (SSU)-rDNA. Conserved primers allowing the amplification of the fungal cox1 gene were designed, and a total of 47 isolates of Zygomycota and Ascomycota were investigated. The analysis revealed a lack of introns in >90% of the isolat ...
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For some smaller operators, the xSPINE can feel a bit too large, even at the smallest settings. For you we have the xSPINE Spacer pads. These cell foam spacer pad offsets the distance between the mesh foam padding and the CF plate. Each pad adds 15mm of distance, and can be stacked with as many as needed.. The pads have velcro hooks on one side, and velcro loops on the opposite side. Simply remove the original mesh foam padding, and insert the spacer pad onto the CF plate, and attach the mesh foam padding onto the spacer pad.. There are three different spacer pads, the front (abdomen), the lower back (lumbar spine), and the upper back (trapezius). Combine them in any way you need to get a perfect fit!. ...
Lactobacillus kefiranofaciens strain ATCC 43761 16S ribosomal RNA gene,partial sequence; 16S-23S intergenic spacer, complete sequence; and 23Sribosomal RNA gene, partial ...
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Overview This course will introduce students to the field of biosystematics and will include history, theory, and practice of both taxonomy (the original bioinformatics) and modern phylogenetics. The methods covered apply equally to all eukaryotic taxa, based on either morphological or molecular characters. Training in key software packages will take place on Fridays. Some of the topics and methods covered:. ...
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The spacers added to the Verifix® MV 760 adhesive guarantee that the required minimum adhesive layer thickness is reached. This makes sure that, when bonding very heavy glass parts, sufficient adhesive is distributed between the bonding parts. Depending on the bonding process, this method has also proven useful when bonding metal in order to maintain the minimum adhesive layer thickness. The spacers do not interfere with the technical properties of the adhesive. Please read the supplied processing guidelines carefully. If you have any questions, our application technicians will be happy to assist you.. ...
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I recently added these wheel spacers from Just Differentials: 1.25 Wheel Spacers Unfortunately I immediately started experiencing significant vibration...
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The internal transcribed spacer 2 database--a web server for (not only) low level phylogenetic analyses. Schultz, J.; Muller, T.; Achtziger, M.; Seibel, P. N.; Dandekar, T.; Wolf, M. in Nucleic Acids Res. (2006). 34 W704--707. ...
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Highly retentive and selective for protonated basic analytes and molecules containing aromatic moities. End-capped pentafluorophenyl with a propyl spacer.
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Kuhls K, Mauricio IL, Pratlong F, Presber W, Schönian G (2005). "Analysis of ribosomal DNA internal transcribed spacer ... The smaller size of the Leishmania BRCA2 DNA repair protein has been exploited to better understand its function in homologous ...
Evidence from nuclear ribosomal DNA internal transcribed spacer sequences". American Journal of Botany. 80 (6): 711-722. doi: ... or the ribosomal spacers ITS) and cladistic analysis in order to investigate the relationships between the family's different ... Lavin M, Doyle JJ, Palmer JD (1990). "Evolutionary significance of the loss of the chloroplast-DNA inverted repeat in the ... These studies have used morphology, DNA data (the chloroplast intron trnL, the chloroplast genes rbcL and matK, ...
5.8S ribosomal DNA and internal transcribed spacer region analyses". Eukaryotic Cell. 6 (1): 110-6. doi:10.1128/EC.00233-06. ...
1: 3-9. Lennon, PA; Cooper CR; Salkin IF; Lee SB (1994). "Ribosomal DNA internal transcribed spacer analysis supports synonymy ... This synonymy has since been confirmed by phylogenetic analysis of the nuclear ribosomal internal transcribed spacer regions. ... of cultures of Scedosporium-like fungi from clinical cases by careful morphological examination and the evaluation of DNA-DNA ...
Evidence from Nuclear Ribosomal DNA Internal Transcribed Spacer Sequences". Am J Bot. 80 (6): 711-722. doi:10.2307/2445441. ... Phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndhF sequences". Plant Syst ... and its Major Subgroups Based on Nuclear Ribosomal DNA ITS and Chloroplast DNA trnL Intron Data". Syst Bot. 24 (3): 409-437. ... molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA". J Agric Food Chem. 51 (23): 6709-6714. doi: ...
... inferred from internal transcribed spacer ribosomal DNA sequences". Canadian Journal of Botany. 77 (1): 150-168. doi:10.1139/ ...
... based on nuclear ribosomal DNA internal transcribed spacer region sequences". American Journal of Botany. Botanical Society of ...
Saghai-Maroof, M.A.; Soliman, K.M.; Jorgensen, R.A. (1984). "Ribosomal DNA spacer-length polymorphisms in barley: mendelian ... Cluster, P.D.; Allard, R.W. (1995). "Evolution of ribosomal DNA (rDNA) genetic structure in colonial Californian populations of ... Wagner, D.B.; Furnier, G.R.; Saghai-Maroof, M.A.; Williams, S.M.; Dancik, B.P.; Allard, R.W. (1987). "Chloroplast DNA ... Neale, D.B.; Saghai-Maroof, M.A.; Allard, R.W.; Zhang, Q.; Jorgensen, R.A. (1988). "Chloroplast DNA diversity in populations of ...
"Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi". Proceedings of the ... Highly conserved DNA sequences are thought to have functional value, although the role for many highly conserved non-coding DNA ... such as ribosomal RNAs, tRNAs and ribosomal proteins are also universally conserved. Sets of conserved sequences are often used ... "Characterization of Bacterial and Fungal Soil Communities by Automated Ribosomal Intergenic Spacer Analysis Fingerprints: ...
Kiss L (July 2012). "Limits of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as species barcodes for Fungi ... The amplification of DNA from environmental samples using general or group-specific primers leads to a mix of genetic material ... 2013), the D1-D3 region of 28S ribosomal RNA genes, may not allow the low taxonomic level classification of the ITS, but ... The development of molecular ecology is also closely related to the use of DNA microarrays, which allows for the simultaneous ...
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ... 1985). "Molecular analysis of the heterogeneity region of the human ribosomal spacer". J. Mol. Biol. 183 (2): 213-223. doi: ...
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ... 1985). "Molecular analysis of the heterogeneity region of the human ribosomal spacer". J. Mol. Biol. 183 (2): 213-223. doi: ...
... based on nuclear ribosomal DNA internal transcribed spacer sequences". American Journal of Botany. 81 (9): 1149-1160. doi: ... During this period, his laboratory methodology changed from working with rRNA to DNA using the polymerase chain reaction (PCR) ...
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... RNA, ribosomal 5, also known as RNR5, is a human gene. Genes for ribosomal RNA are clustered on the short arms of chromosomes ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ...
Gonzalez IL, Sylvester JE (1995). "Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer ... McCallum FS, Maden BE (1986). "Human 18 S ribosomal RNA sequence inferred from DNA sequence. Variations in 18 S sequences and ... Sylvester JE, Petersen R, Schmickel RD (1990). "Human ribosomal DNA: novel sequence organization in a 4.5-kb region upstream ... 1985). "Molecular analysis of the heterogeneity region of the human ribosomal spacer". J. Mol. Biol. 183 (2): 213-223. doi: ...
... using the first internal transcribed spacer and the D1-D3 expansion segments of 28S ribosomal DNA. Journal of Nematology 40(3 ...
2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of ...
... evidence from nuclear ribosomal DNA internal transcribed spacer sequences" (PDF). American Journal of Botany. 87 (1): 76-95. ...
High-resolution phylogeny for Helianthus (Asteraceae) using the 18S-26S ribosomal DNA external transcribed spacer. American ...
... based on internal transcribed spacer sequences of ribosomal DNA". Korean Journal of Genetics. 20 =issue=2: 109-116. The idea ... conducted the phylogenetic analysis using sequences from both rbcL gene and trnL-trnF intergenic spacer of chloroplast DNA and ... September 2002). "Phylogenetic Analysis of Korean Prunus ( Rosaceae ) Based on ITS Sequences of Nuclear Ribosomal DNA". Gene & ... markers and sequence analysis of two chloroplast DNA genes, rpl16 and trnL-trnF spacer and showed that king cherry can be ...
Phylogenetic relationships in Helianthus (Asteraceae) based on nuclear ribosomal DNA internal transcribed spacer region ...
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... Her cladistic analysis combined comparisons of the sequences of the internal transcribed spacer in the non-functional RNA with ...
... of nuclear ribosomal DNA". American Journal of Botany. 86 (7): 1027-1037. doi:10.2307/2656620. JSTOR 2656620. PMID 10406726. Li ... "Phylogenetic relationships of the Hamamelidaceae inferred from sequences of internal transcribed spacers (ITS) ... "Phylogeny of the Hamamelidaceae based on the ITS sequences of nuclear ribosomal DNA". Biochemical Systematics and Ecology. 26 ( ... Morphological and DNA studies have supported monophyly of the Hamamelidoideae and have recognized the separation of the ...
Based on Nuclear Ribosomal Internal Transcribed Spacer and Chloroplast trnL-F Sequences. In Aliso: A Journal of Systematic and ... based on chloroplast and nuclear DNA sequences. Telopea, 13(1-2), 115-142. Soreng, R. J., Peterson, P. M., Romaschenko, K., ...
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... The cladistic analysis combined comparison of sequences from the internal transcribed spacer in the non-functional RNA with ...
... based on the internal transcribed spacer of the nuclear ribosomal DNA. Plant Systematics and Evolution, 256(1), 1-16. Tsai, C. ...
... relationships within Sarcoscypha based upon nucleotide sequences of the internal transcribed spacer of nuclear ribosomal DNA". ... The cladistic analysis combined comparison of sequences from the internal transcribed spacer in the non-functional RNA with ...
... of DNA Extraction Methods for Microbial Community Analysis in Indonesian Tempe Employing Amplified Ribosomal Intergenic Spacer ... DNA sequence and mechanism of transfer". Journal of Bacteriology. 182 (1): 81-90. doi:10.1128/jb.182.1.81-90.2000. PMC 94243. ...
... based on internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA" (PDF). American Journal of Botany. 86 (4): 590- ... 2005). 2C DNA variation and relationships among New World species of the genus Lupinus (Fabaceae). Plant Systematics and ...
Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer ...
... genome sizes Human genome Junk DNA List of sequenced eukaryotic genomes Non-coding DNA Plant DNA C-values Database Selfish DNA ... "Psyllid endosymbionts exhibit patterns of co-speciation with hosts and destabilizing substitutions in ribosomal RNA". Insect ... dispersed in the ancestor gene but aggregated and the negative relation between number of lost genes and length of the spacers ... Some single-celled organisms have much more DNA than humans, for reasons that remain unclear (see non-coding DNA and C-value ...
... based on plastid matK and nuclear ribosomal spacer (ITS) DNA sequences and morphology", Plant Systematics and Evolution, 246 (3 ...
The conclusions of this analysis, based on the DNA sequences of ribosomal DNA coding for internal transcribed spacers, are ...
"Six group I introns and three internal transcribed spacers in the chloroplast large subunit ribosomal RNA gene of the green ... They act on the cellular DNA of the cell that synthesizes them; to be precise, in the opposite allele of the gene that encode ... Three internal transcribed spacers and 12 group I intron insertion sites". J Mol Biol. 232 (2): 446-67. doi:10.1006/jmbi. ... Turmel M, Mercier JP, Côté V, Otis C, Lemieux C (July 1995). "The site-specific DNA endonuclease encoded by a group I intron in ...
Hofmann K (April 2009). "Ubiquitin-binding domains and their role in the DNA damage response". DNA Repair. 8 (4): 544-56. doi: ... UBA52 and RPS27A genes code for a single copy of ubiquitin fused to the ribosomal proteins L40 and S27a, respectively. The UBB ... The UBDs exploit this by having small spacers between ubiquitin-interacting motifs that bind lysine 48-linked chains (compact ... Monoubiquitinated PCNA recruits polymerases that can carry out DNA synthesis with damaged DNA; but this is very error-prone, ...
... inferred from nuclear ribosomal DNA Internal transcribed spacers data (nrDNA ITS)". Ecologia Mediterranea. 36 (1): 99-106. doi: ...
... five represented the same species after comparing the DNA sequences of the internal transcribed spacer region of ribosomal DNA ... marginata based on ribosomal DNA sequences and restriction fragment length polymorphism analyses. Because of differences in ... The amatoxins inhibit the enzyme RNA polymerase II, which copies the genetic code of DNA into messenger RNA molecules. The ... List of deadly fungi Gulden G, Dunham S, Stockman J (2001). "DNA studies in the Galerina marginata complex". Mycological ...
Pre-mRNA sequence of miR-712 is generated from the murine ribosomal RN45s gene at the internal transcribed spacer region 2 ( ... Defects in DNA repair cause the accumulation of mutations, which can lead to cancer. Several genes involved in DNA repair are ... Cancer is caused by the accumulation of mutations from either DNA damage or uncorrected errors in DNA replication. ... DNA and Cell Biology. 26 (4): 209-18. doi:10.1089/dna.2006.0545. PMID 17465887. Peterson KJ, Dietrich MR, McPeek MA (July 2009 ...
Based on Gen Internal Transcribed Spacer (ITS1 AND ITS2), 5.8 DNA Ribosomal. In Proceeding International Conference on Global ...
Achenbach-Richter, L; Woese CR (1988). "The ribosomal gene spacer region in archaebacteria". Syst. Appl. Microbiol. 10 (3): 211 ... "DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA". Int. J. Syst. Evol. Microbiol. 50 (3): 1095- ... Winker, S; Woese CR (1991). "A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA ... Hansmann, S; Martin W (2000). "Phylogeny of 33 ribosomal and six other proteins encoded in an ancient gene cluster that is ...
... of Nuclear Ribosomal DNA and the trnL/F Region of Chloroplast DNA" (PDF), International Journal of Plant Sciences, 164 (2): 197 ... 2003), "The Phylogeny of Rosoideae (Rosaceae) Based on Sequences of the Internal Transcribed Spacers (ITS) ...
... based on ribosomal DNA internal transcribed spacer (ITS) sequences", American Journal of Botany, 84 (7): 956-965, doi:10.2307/ ... DNA studies have shown that, despite not having a twisted fruit, African violets evolved from within the Tanzanian ...
... based on plastid matK and nuclear ribosomal spacer (ITS) DNA sequences and morphology", Plant Systematics and Evolution, 246 (3 ...
Recently, DNA barcoding of the internal transcribed spacer (ITS) region of C. bertholletiae ribosomal DNA was performed to ... "DNA barcoding of clinically relevant Cunninghamella species". Medical Mycology. 53 (2): 99-106. doi:10.1093/mmy/myu079. PMID ...
... based on sequences from the internal transcribed spacers (ITS) of nuclear ribosomal DNA". American Journal of Botany. 85 (12): ... after analyzing and matching the internal transcribed spacers of nuclear ribosomal DNA from many Seriphidium and Artemisia ... Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS)". Plant Biology. 5 (3): 274-84. doi: ... Chloroplast and ribosomal DNA sequence analysis in 2011 supported monophyly with two clades, one of which includes some North ...
The ribosomal DNA contains one transcriptional unit with a cluster of genes coding for the 18S, 5.8S and 28S rRNAs and two ... Identification of phytoplasma strain-specific PCR primers obtained from 16S/23S rRNA spacer sequences. (1996). Appl. Environ. ... Though reliable, DNA hybridization, electron microscopy and PCR techniques require specialized equipment and trained human ... Ahrens, U., and Seemüller, E. (1992) Detection of DNA of plant pathogenic mycoplasma-like organisms by a polymerase chain ...
Based on Sequence Data from Internal Transcribed Spacers (ITS) of Nuclear Ribosomal DNA", Lindleyana 15(2): 96-114. 2000. The ...
... region of nuclear ribosomal DNA", Systematic Botany, 27 (1): 127-137, doi:10.1043/0363-6445-27.1.127 (inactive 31 July 2022){{ ... 2002), "Monophyly and phylogeny of Monarda (Lamiaceae): Evidence from the internal transcribed spacer (ITS) ...
... using internal transcripted spacer sequences of nuclear ribosomal DNA: Implications for biogeography and concerted evolution". ...
Insights from internal transcribed spacer sequences of nuclear ribosomal DNA". Genome. 40 (5): 730-743. doi:10.1139/g97-796. ...
In one application an artificial DNA catalyst was prepared by attaching a copper ion to it through a spacer. The copper - DNA ... autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena". Cell. 31 (1): 147-157. doi:10.1016/ ... Other uses of DNA in chemistry are in DNA-templated synthesis, Enantioselective catalysis, DNA nanowires and DNA computing. ... Deoxyribozymes, also called DNA enzymes, DNAzymes, or catalytic DNA, are DNA oligonucleotides that are capable of performing a ...
Nina Rønsted and colleagues analysed the DNA sequences from the nuclear ribosomal internal and external transcribed spacers, ...
This was not known, as the distribution of ORF lengths in a random DNA sequence had never been studied. As random DNA sequences ... The theory correctly predicts that splice junctions present in transfer RNA genes and ribosomal RNA genes, do not contain stop ... In their view, introns originated as spacer sequences that enabled convenient recombination and shuffling of exons that encoded ... If split genes originated from random primordial DNA sequences, they would contain a significant amount of DNA that represented ...
Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. In: Proceedings of the ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. / Schoch, Conrad L.; ... title = "Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi", ...
DNA, Ribosomal Spacer ... Additionally, T. asahii DNA was detected stably from skin ... Of the nine genotypes of the intergenic spacer region of the T. asahii rRNA gene, type 1 predominated (81.7%), followed by ...
DNA CATALÍTICO. DNA, CATALYTIC. ADN CATALITICO. DNA ESPAÇADOR RIBOSSÔMICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN ... DNA INTERGÊNICO. DNA, INTERGENIC. ADN INTERGENICO. DOENÇAS TRANSMISSÍVEIS EMERGENTES. COMMUNICABLE DISEASES, EMERGING. ... ENSAIO DE AMPLIFICAÇÃO DE SINAL DE DNA RAMIFICADO. BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ...
Definitive identification was achieved using the ribosomal DNA (rDNA) sequence analysis by targeting the ITS1 (internal ... transcribed spacer 1) region. This case report is intended to promote awareness of this fungus as a potential pathogen, by ... mitochondrial DNA genomes inferred two clades of plasmodia in orangutans (Pongo 1 and Pongo 2), and that these clades likely ... Of all biospecimen types reported in the literature, the most frequently used were DNA, postmortem spinal cord, blood, and ...
Rapid identification of bacteria on the basis polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Applied ... Zhang, Z., Schwartz, S., Wagner, L., & Miller, W. (2000). A greedy algorithm for aligning DNA sequences. Journal of ... White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for ... Halstensen, A. S. (2008). Species-specific fungal DNA in airborne dust as surrogate for occupational mycotoxin exposure. ...
... internal transcribed spacers; mycelium; pathogenicity; pathogens; peptide elongation factors; ribosomal DNA; sand; soil; spring ... Camellia sinensis; DNA-directed RNA polymerase; Fusarium solani; air drying; autoclaving; bark; conidia; culture media; ... DNA; Ficus carica; Lasiodiplodia theobromae; alcohols; ambient temperature; cell walls; conidia; culture media; data collection ... DNA; Neoscytalidium dimidiatum; Olea europaea; Turkey (country); bark; conidia; cultivars; dieback; disease control; disease ...
Based on Nucleotide Sequences of Nuclear Ribosomal DNA Internal Transcribed Spacers (ITS). *J. Vallès, M. Torrell, T. Garnatje ... A flow cytometric study of nuclear DNA content in a wide range of taxa of the Asteraceae family is carried out, filling gaps in ... THE CARDUEAE (COMPOSITAE) REVISITED: INSIGHTS FROM ITS, trnL-trnF, AND matK NUCLEAR AND CHLOROPLAST DNA ANALYSIS1, 2. *A. ... Generic Delimitation and Phylogeny of the Subtribe Centaureinae (Asteraceae): A Combined Nuclear and Chloroplast DNA Analysis. ...
In this study, we analyzed the Leishmania DNA using nuclear ribosomal internal transcript spacer (ITS) sequences. Parasite DNA ... DNA of R. parkeri was detected in a total of 44 (24%) of these ticks. DNA of "Candidatus Rickettsia andeanae" and Rickettsia ... In addition, we developed a simple protocol to extract DNA from sonicated first-instar larvae, and used that DNA to test the ... The lower limit of detection for our assay is equivalent to ~0.03 of a first-instar larva in a 60-μl sample (0.016 ng of DNA ...
Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. Amer. J. Bot. 86: 81 ...
Trichosporon asahii genotypes determined on the basis of ribosomal DNA intergenic spacer 1 sequencing, and antifungal ... Invasive Trichosporon infection in solid organ transplant patients: a report of two cases identified using IGS1 ribosomal DNA ... DNA micro-array based detection and identification of fungal pathogens in clinical samples from neutropenic patients. J Clin ...
PCR method was based on specific DNA nucleotide differences in the intergenic spacer of ribosomal DNA (rDNA) [11]. The ... The DNA was extracted from a single mosquito using a DNA extraction kit (Qiagen) with 1 uL of the DNA amplified in a 24 uL PCR ... and 0.9 U DNA polymerase (AmpliTaq). The PCR condition with an initial step of 10 min at 94°C, followed by 30 cycles each ...
The intergenic spacer region of the ribosomal RNA gene of Penicillium marneffei shows almost no DNA sequence diversity. ...
... assay or of Bartonella antigens targeting the 16S-23S ribosomal RNA gene intergenic transcribed spacer via immunohistochemical ... 4] Note that a negative result suggests the absence of detectable DNA but does not rule out Bartonella infection. The PCR assay ... Detection of Bartonella DNA in tissue specimens via polymerase chain reaction (PCR) ... Multiplexed colorimetric detection of Kaposis sarcoma associated herpesvirus and Bartonella DNA using gold and silver ...
... and ITS4 regions of nuclear ribosomal DNA. A total of 66 endophytic fungi were successfully isolated from different parts of A ... trees based on the concatenated CDS and internal transcribed spacer (ITS) sequences that were analyzed separately have revealed ... Endophytic fungal identification was conducted by PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) ...
... yeasts in floor dust of 50 elementary schools characterized with sequencing internal transcribed spacer region of ribosomal DNA ... Terms: DNA or Deoxyribonucleic acid OR DNA or Deoxyribonucleic acid 1 - 10 of 581 Bibliographic entries ... Global DNA methylation of WTC prostate cancer tissues show signature differences compared to non-exposed cases. ... Global DNA methylation profiles in peripheral blood of WTC-exposed community members with breast cancer. ...
We determined the internal transcribed spacers of ribosomal DNA (ITS rDNA) sequences of these nematodes. The divergence ( ... MULTI-STATE OCCUPANCY MODEL ESTIMATES PROBABILITY OF DETECTION OF AN AQUATIC PARASITE USING ENVIRONMENTAL DNA: PSEUDOLOMA ...
Intraspecific variation in the first internal transcribed spacer (ITS1) of the nuclear ribosomal DNA inMelipona subnitida( ...
Characterization of group I intron-like insertion elements in shark ribosomal DNA spacers.. ... A set of insertion elements has been found in the ribosomal DNA (rDNA) internal transcribed spacers in four Orders of sharks ( ... The use of PCR-RFLP and DNA sequence analysis of major histocompatibility class II alpha genes for paternity testing in the ... Microsatellite DNA profiling in the blue shark Prionace glauca : development and potential application for population studies. ...
... region of nuclear ribosomal DNA is the most frequently used marker for distinguishing plant species. Previously published ... region of nuclear ribosomal DNA is the most frequently used marker for distinguishing plant species. Previously published ... could not preferentially amplify plant DNA or discriminate against fungal DNA. For P. tongkangensis, the dismatch of ITS5 ... could not preferentially amplify plant DNA or discriminate against fungal DNA. For P. tongkangensis, the dismatch of ITS5 ...
Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA. Theor. Applied ... Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA in Winteraceae. Am ... DNA amplification profile using PE [F and R] primers. Lanes M-100 bp DNA ladder, PL-Phyllanthus emblica DNA from leaves. PF- ... DNA extraction, ITS amplification and sequencing: Total genomic DNA was isolated from leaf materials following the DNA ...
The nucleotide sequence of the 16S-23S ribosomal RNA intergenic spacer obtained from 1 genotype (clone F17688) was nearly ... 9) amplified Bartonella DNA by using PCR with Pulex spp. fleas collected from persons in Peru and suggested the existence of a ... Each flea was crushed with a sterile pestle, and DNA was extracted by lysis with 0.7 M ammonium hydroxide. PCR was used to ... sterile water instead of template DNA) were used. PCR products were purified, and both strands of each amplicon were subjected ...
Multigene phylogenies (internal transcribed spacer ribosomal DNA, ß-tubulin, and RNA polymerase II) of isolates recovered from ...
Resolution of Prochlorococcus and Synechococcus ecotypes by using 16S-23S ribosomal DNA internal transcribed spacer sequences. ... DNA base composition and genome size of the prokaryotic symbiont in Riftia pachyptila (Pogonophora). FEMS Microbiol. Let. 24: ... Trichodesmium genome maintains abundant, widespread noncoding DNA in situ, despite oligotrophic lifestyle. PNAS 112: 4251-4256 ...
... region of Biomphalaria ribosomal DNA (rDNA) using polymerase chain reaction amplification (PCR) and restriction fragment length ... Kane RA, Rollinson D. Repetitive sequences in the ribosomal DNA internal transcribed spacer of Schistosoma haematobium, ... We analyzed the internal transcribed spacer 2 (ITS2) region of Biomphalaria ribosomal DNA (rDNA) using polymerase chain ... reinvestigated using nuclear ribosomal DNA internal transcribed spacer sequences. Can J Zool. 1997;75:1540-5. ...
In addition, the mt genome-wide base modification pattern of O. sinensis was determined in this study, especially DNA ... In addition, the mt genome-wide base modification pattern of O. sinensis was determined in this study, especially DNA ... genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt ... genome of Ophiocordycpes sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt ...
Complex communities of fungi are regularly characterised by metabarcoding using the Internal Transcribed Spacer (ITS) and the ... Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the ... There is broad agreement that the internal transcribed spacer (ITS) of the nuclear rRNA gene cluster should be the standard DNA ... Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several ...
Initial phylogenetic analyses of DNA sequence data from the nuclear ribosomal internal transcribed spacers (ITS) for 24 ...
Our findings also suggest that RNA-directed DNA methylation is required to silence isolated elements that may be too small to ... In Arabidopsis, DNA cytosine methylation is maintained by three subfamilies of methyltransferases with distinct substrate ... We used a microarray-based profiling method to explore the involvement of Arabidopsis CMT3 and DRM DNA methyltransferases, a ... We conclude that the targets of both DNA methylation and histone H3K9 methylation pathways are transposable elements genome- ...
  • We determined the internal transcribed spacers of ribosomal DNA (ITS rDNA) sequences of these nematodes. (allenpress.com)
  • Studies of restriction site variation in rDNA in populations of animals and plants have shown that while coding regions are conserved, these spacer regions are variable ( Gerbi,1985 ). (scialert.net)
  • We analyzed the internal transcribed spacer 2 (ITS2) region of Biomphalaria ribosomal DNA (rDNA) using polymerase chain reaction amplification (PCR) and restriction fragment length polymorphism (RFLP). (biomedcentral.com)
  • We reidentify three rare yeasts from a culture collection of onychomycosis agents by matrix-assisted laser desorption/ionization time of flight/mass spectrometry (MALDI-TOF/MS) and sequencing the internal transcribed spacer (ITS) regions or the intergenic spacer (IGS) 1 region of ribosomal DNA (rDNA), and present their enzymatic and antifungal susceptibility profiles. (em-consulte.com)
  • We analyse intragenomic variation of the ITS2 internal transcribed spacer of ribosomal DNA (rDNA) in the grasshopper Eyprepocnemis plorans, by means of tagged PCR 454 amplicon sequencing performed on both genomic DNA (gDNA) and RNA-derived complementary DNA (cDNA), using part of the ITS2 flanking coding regions (5.8S and 28S rDNA) as an internal control for sequencing errors. (umn.edu)
  • We studied the organization of rDNA spacer sequences within several rDNA loci in D. melanogaster. (elsevier.com)
  • Every locus showed many discrete length classes of rDNA spacer, ranging from 2.4 kb to about 20 kb. (elsevier.com)
  • Only a small fraction of a typical eukaryotic nuclear genome constitutes rDNA or protein-coding genes, whereas a large fraction of all higher eukaryotic genomes consists of repetitive sequences and heterochromatic satellite DNA (satDNA) [ 1 , 2 ]. (biomedcentral.com)
  • The sequencing of the 16S-23S ribosomal RNA intergenic spacer region (ITS) showed that all bacteria belonged to the Bradyrhizobium genus. (springernature.com)
  • Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. (bvsalud.org)
  • Internal transcribed spacer regions are sequences located in eukaryotic rRNA genes between the 18S and 5.8S rRNA coding regions (ITS1) and between the 5.8S and 25S (26S) rRNA coding regions (ITS2). (scialert.net)
  • In fungi and plants, however, the localized nature of DNA methylation makes it possible to identify sequences that are targeted for DNA methylation. (biomedcentral.com)
  • For example, in Neurospora , DNA methylation occurs at repeated sequences that are targeted for point mutation [ 5 ]. (biomedcentral.com)
  • The Dnmt3 subfamily of DNA methyltransferases, which includes Arabidopsis DRM1 and DRM2, can methylate de novo [ 9 ], but there are no known sequences in common among target sites. (biomedcentral.com)
  • The CV method performs well in grouping effectiveness of DNA barcode sequences, as compared to K2P analysis of aligned sequences. (biomedcentral.com)
  • Species were identifi ed on the basis of source of infection by entities emerging on the human internal transcribed spacer (ITS), partial cell division cycle host is essential for understanding the evolution of ( CDC42 ), -tubulin ( BT2 ), and ACT sequences ( 10-14 ). (cdc.gov)
  • The hyrcanus group of Anopheles ( Anopheles ) in China (Diptera: Culicidae): Species discrimination and phylogenetic relationships inferred by ribosomal DNA internal transcribed spacer 2 sequences. (si.edu)
  • Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses. (psu.ac.th)
  • DNA sequences may be stored in databases accessible over the internet, obviating the need for the exchange of reference strains. (biomedcentral.com)
  • Typing procedures based on DNA sequences overcome these limitations, since sequence data may easily be exchanged and stored in databases that are accessible via the internet. (biomedcentral.com)
  • The nuclear ribosomal large subunit, a popular phylogenetic marker in certain groups, had superior species resolution in some taxonomic groups, such as the early diverging lineages and the ascomycete yeasts, but was otherwise slightly inferior to the ITS. (illinois.edu)
  • In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. (edu.hk)
  • Three subunits from the nuclear ribosomal RNA cistron were compared together with regions of three representative protein-coding genes (largest subunit of RNA polymerase II, second largest subunit of RNA polymerase II, and minichromosome maintenance protein). (illinois.edu)
  • The nuclear ribosomal small subunit has poor species-level resolution in fungi. (illinois.edu)
  • Ceballos-Escalera A, Richards J, Arias MB, Inward DJG, Vogler AP (2022) Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU) rRNA markers. (pensoft.net)
  • Complex communities of fungi are regularly characterised by metabarcoding using the Internal Transcribed Spacer ( ITS ) and the Large-Subunit ( LSU ) gene of the rRNA locus, but reliance on a single short sequence fragment limits the confidence of identification. (pensoft.net)
  • In 2003, Hebert et al [ 1 ] proposed to use a 648-bp region from the 5'-end of the cytochrome c oxidase subunit 1 (COI) gene as a DNA barcode for identifying all metazoan species. (biomedcentral.com)
  • We evaluated the systematics of these taxa with phylogenetic analyses of DNA sequence data from the nuclear ribosomal regions of the internal transcribed spacer (ITS) and 28S large subunit (LSU). (northwestern.edu)
  • Based on morphology and DNA sequence data of the large subunit nuclear ribosomal RNA gene (LSU, 28S) and the internal transcribed spacers (ITS) and 5.8S rRNA gene of the nrDNA operon, the genus Tiarosporella is shown to be poly- and paraphyletic. (up.ac.za)
  • Although the protein-coding gene regions often had a higher percent of correct identification compared with ribosomal markers, low PCR amplification and sequencing success eliminated them as candidates for a universal fungal barcode. (illinois.edu)
  • The absence of all potential vectors of human Plas- an internal transcribed spacer 2 gene sequence analysis modium spp. (cdc.gov)
  • Detection of Bartonella DNA in tissue specimens via polymerase chain reaction (PCR) assay or of Bartonella antigens targeting the 16S-23S ribosomal RNA gene intergenic transcribed spacer via immunohistochemical methods is diagnostic. (medscape.com)
  • DSB-repair within the ribosomal RNA tandem gene-repeats is likely dominated by single-strand annealing allowing approximately 80% of cells to survive the break. (pasteur.fr)
  • Our goal was to clarify ancestral relationships, investigate the position of Dalibarda repens relative to Rubus, and examine the origin of the circumboreal, octoploid species R. chamaemorus using sequence data from one additional chloroplast DNA region, trnS-trnG, and the singlecopy nuclear gene Granule-Bound Starch Synthase (GBSSI-1). (wku.edu)
  • We cloned and sequenced 18S ribosomal DNA and the internal transcribed spacer-1 (ITS-1) gene. (marquette.edu)
  • Endophytic fungal identification was conducted by PCR amplification and sequencing of the internal transcribed spacer 1 (ITS1) and ITS4 regions of nuclear ribosomal DNA. (researchgate.net)
  • Previously published polymerase chain reaction (PCR) primers available for amplifying the ITS region from environmental plant samples provide a high degrees of success while maintaining a broad range of compatibility difficult to discriminate against fungal DNA. (academicjournals.org)
  • Thus, the plant-specific ITS primer design is required to achieve the preferential amplification of plant DNA with discrimination against fungal DNA from environmental plant samples. (academicjournals.org)
  • Our results showed that ITS4-R, when paired with either a universal primer ITS5 or the newly designed primer ITS1-F2, efficiently amplified DNA from environmental P. cernua samples and discriminated against parasitic fungal DNAs, while another newly designed primer ITS1-F2, when paired with either a universal primer ITS4 or ITS4-R, could not preferentially amplify plant DNA or discriminate against fungal DNA. (academicjournals.org)
  • For P. tongkangensis , the dismatch of ITS5 primer resulted in noneffective or wrong amplification when paired with either ITS4-R or ITS4, while ITS1-F2, when paired with ITS4-R and ITS4, efficiently amplified plant DNA and both plant and fungal DNAs, respectively. (academicjournals.org)
  • COI has several claims to be a suitable DNA barcode marker, including ease in amplification across a wide variety of organisms and provision of enough information to enable organisms to be identified to the species level. (biomedcentral.com)
  • Multigene phylogenies (internal transcribed spacer ribosomal DNA, ß-tubulin, and RNA polymerase II) of isolates recovered from these vineyards indicated that Eutypa dieback is caused primarily by an undescribed Eutypa sp. (usda.gov)
  • Comparisons of isolates of Heterodera avenae using 2-D PAGE protein patterns and ribosomal DNA. (plantprotection.pl)
  • We investigated genetic variation among 52 morphologically and geographically diverse, indoor and outdoor isolates of S. chartarum sensu lato using molecular markers based on β-tubulin, calmodulin, elongation factor-1 alpha, and trichodiene synthase genes, as well as the internal transcribed spacer region of nuclear ribosomal DNA. (sporometrics.com)
  • The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. (bvsalud.org)
  • Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. (illinois.edu)
  • Among the regions of the ribosomal cistron, the internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi, with the most clearly defined barcode gap between inter- and intraspecific variation. (illinois.edu)
  • in Europe (Finland, Norway and Switzerland) and differs from D. parca according to a five-locus alignment consisting of ITS, LSU, partial 18S nuclear ribosomal RNA, translation elongation factor 1- alpha and beta-tubulin genes. (up.ac.za)
  • Variation in spacer ribosomal DNA in some cyst- forming species of plant parasitic nematodes. (plantprotection.pl)
  • This report presents results from a systematic screen for variation in repetitive DNA in the genome of C. difficile . (biomedcentral.com)
  • The internal transcribed spacer (ITS) region of nuclear ribosomal DNA is the most frequently used marker for distinguishing plant species. (academicjournals.org)
  • It is hypothesized that genome size in polyploids tends to a maximum as it follows saturation behaviour, in agreement with the Michaelis-Menten model, and is suggested a theoretical upper limit for the DNA content of this genus. (semanticscholar.org)
  • DNA base composition and genome size of the prokaryotic symbiont in Riftia pachyptila (Pogonophora). (whoi.edu)
  • Single molecule, real-time (SMRT) sequencing was used to characterize mitochondrial (mt) genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. (frontiersin.org)
  • In addition, the mt genome-wide base modification pattern of O. sinensis was determined in this study, especially DNA methylation. (frontiersin.org)
  • We conclude that the targets of both DNA methylation and histone H3K9 methylation pathways are transposable elements genome-wide, irrespective of element type and position. (biomedcentral.com)
  • Internal Transcribed Spacer (ITS) 1 and 2 were sequenced for five species of the genus Phyllanthus namely P. emblica , P. reticulatus , P. amarus , P. fraternus and P. urinaria , with a view to identify accurately herbaceous species. (scialert.net)
  • Non-coding satellite DNA (satDNA) usually has a high turn-over rate frequently leading to species specific patterns. (biomedcentral.com)
  • The results confirm that methodological problems posed by RAPD markers can be avoided by careful laboratory procedures and appropriate data analyses and suggest that this kind of marker is useful at low taxonomic levels and is, furthermore, complementary to DNA sequence analysis. (semanticscholar.org)
  • RAPD (Random Amplified Polymorphic DNA) markers have been most widely used for this purpose 8-10 . (bvsalud.org)
  • Quality of genomic DNA was verifi ed growing on plant debris. (cdc.gov)
  • PCR was used to detect Bartonella DNA (according to the defining criteria for Bartonella spp. (cdc.gov)
  • 9 ) amplified Bartonella DNA by using PCR with Pulex spp. (cdc.gov)
  • A systematic assessment of cell type deconvolution algorithms for DNA methylation data. (cdc.gov)
  • Global DNA methylation of WTC prostate cancer tissues show signature differences compared to non-exposed cases. (cdc.gov)
  • Global DNA methylation profiles in peripheral blood of WTC-exposed community members with breast cancer. (cdc.gov)
  • DNA methylation occurs at preferred sites in eukaryotes. (biomedcentral.com)
  • In Arabidopsis , DNA cytosine methylation is maintained by three subfamilies of methyltransferases with distinct substrate specificities and different modes of action. (biomedcentral.com)
  • Our findings also suggest that RNA-directed DNA methylation is required to silence isolated elements that may be too small to be maintained in a silent state by a chromatin-based mechanism alone. (biomedcentral.com)
  • DNA cytosine methylation is an ancient process, found in both prokaryotes and eukaryotes, and catalyzed by a single family of methyltransferases [ 1 ]. (biomedcentral.com)
  • In prokaryotes, cytosine-5 methyltransferases protect target sites from cleavage by partner restriction endonucleases, but in eukaryotes, the function of DNA methylation is less clear. (biomedcentral.com)
  • An additional form of DNA methylation is found in the model plant Arabidopsis , where short dense CG methylation clusters are occasionally found in genic regions that are otherwise devoid of methylation [ 8 ]. (biomedcentral.com)
  • Although many DNA methylation targets are known, it has been unclear how these sites are recognized by DNA methyltransferases. (biomedcentral.com)
  • however, the mechanism by which siRNA production leads to de novo DNA methylation is not known. (biomedcentral.com)
  • Another open question is how some forms of DNA methylation are maintained during rounds of cell division. (biomedcentral.com)
  • In the case of CG sites, a member of the Dnmt1 subfamily of DNA methyltransferases maintains methylation by specifically methylating hemi-methylated sites behind the replication fork [ 15 ], but in cases of non-CG methylation, there does not appear to be a comparable reaction. (biomedcentral.com)
  • Non-CG methylation in Neurospora is maintained by the action of a histone H3 lysine-9 (H3K9) methyltransferase [ 5 ], so the successive action of a histone methyltransferase and a DNA methyltransferase suffices to maintain methylation indefinitely. (biomedcentral.com)
  • 4 ) the internal transcribed spacer regions and the partial cell and Yëgues-Rodriguez et al. (cdc.gov)
  • In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 11 populations. (plantprotection.pl)
  • By analyzing nucleotide polymorphism of the chloroplast, we propose 21 hypervariable regions as potential DNA barcode regions for Myrtales. (biomedcentral.com)
  • It was found that internal transcribed spacer 1 showed more potential than internal transcribed spacer 2 in assessing their relationship at molecular level. (scialert.net)
  • Here, we further explored the grouping effectiveness of the CV method in large DNA barcode datasets (COI, 18S and 16S rRNA) from a variety of organisms, including birds, fishes, nematodes and crustaceans. (biomedcentral.com)
  • Scatter plots and statistical tests revealed a significant correlation between genetic distances for satDNA and mitochondrial DNA. (biomedcentral.com)
  • We have explored the effect of DSBs on targeted integration in bloodstream-form Trypanosoma brucei, focusing on the ribosomal RNA-spacer target commonly used to integrate recombinant constructs. (pasteur.fr)
  • We conclude that the CV method is a fast and reliable method for analyzing large datasets for DNA barcoding purposes. (biomedcentral.com)
  • Discrepancies between prokaryotes and eukaryotes need to be considered in soil DNA-based studies. (myxotropic.org)
  • We used a microarray-based profiling method to explore the involvement of Arabidopsis CMT3 and DRM DNA methyltransferases, a histone H3 lysine-9 methyltransferase (KYP) and an Argonaute-related siRNA silencing component (AGO4) in methylating target loci. (biomedcentral.com)
  • A flow cytometric study of nuclear DNA content in a wide range of taxa of the Asteraceae family is carried out, filling gaps in some less studied groups. (semanticscholar.org)
  • 3α-Angeloyloxy-ent-kaur-16-en-19-oic acid (WT-25) is an ent-kaurane dieterpenoid extracted from Wedelia trilobata, a medicinal plant with potential anti-inflammatory activity. (bvsalud.org)