In vivo expression of the nucleolar group I intron-encoded I-dirI homing endonuclease involves the removal of a spliceosomal intron. (1/10320)

The Didymium iridis DiSSU1 intron is located in the nuclear SSU rDNA and has an unusual twin-ribozyme organization. One of the ribozymes (DiGIR2) catalyses intron excision and exon ligation. The other ribozyme (DiGIR1), which along with the endonuclease-encoding I-DirI open reading frame (ORF) is inserted in DiGIR2, carries out hydrolysis at internal processing sites (IPS1 and IPS2) located at its 3' end. Examination of the in vivo expression of DiSSU1 shows that after excision, DiSSU1 is matured further into the I-DirI mRNA by internal DiGIR1-catalysed cleavage upstream of the ORF 5' end, as well as truncation and polyadenylation downstream of the ORF 3' end. A spliceosomal intron, the first to be reported within a group I intron and the rDNA, is removed before the I-DirI mRNA associates with the polysomes. Taken together, our results imply that DiSSU1 uses a unique combination of intron-supplied ribozyme activity and adaptation to the general RNA polymerase II pathway of mRNA expression to allow a protein to be produced from the RNA polymerase I-transcribed rDNA.  (+info)

p53 represses ribosomal gene transcription. (2/10320)

Induction of the tumor suppressor protein p53 restricts cellular proliferation. Since actively growing cells require the ongoing synthesis of ribosomal RNA to sustain cellular biosynthesis, we studied the effect of p53 on ribosomal gene transcription by RNA polymerase I (Pol I). We have measured rDNA transcriptional activity in different cell lines which either lack or overexpress p53 and demonstrate that wild-type but not mutant p53 inhibits cellular pre-rRNA synthesis. Conversely, pre-rRNA levels are elevated both in cells which express mutant p53 and in fibroblasts from p53 knock-out mice. Transient transfection assays with a set of rDNA deletion mutants demonstrate that intergenic spacer sequences are dispensable and the minimal rDNA promoter is sufficient for p53-mediated repression of Pol I transcription. However, in a cell-free transcription system, recombinant p53 does not inhibit rDNA transcription, indicating that p53 does not directly interfere with the basal Pol I transcriptional machinery. Thus, repression of Pol I transcription by p53 may be a consequence of p53-induced growth arrest.  (+info)

A new rapidly growing mycobacterial species, Mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre. (3/10320)

Scotochromogenic mycobacterial isolates from water-damaged parts of indoor building materials of a children's day care centre represented a phenetically and genetically distinct group of strains. A 16S rDNA dendrogram (1243 bp) showed that the closest species to the new strain MA112/96T was Mycobacterium abscessus. Phylogenetic and phenetic analyses (100 characteristics) grouped the new isolates with M. abscessus, Mycobacterium vaccae, Mycobacterium aurum and Mycobacterium austroafricanum. Ribotyping with Pvull restriction distinguished the 5 isolates from the other 12 most closely related species by the major bands at 6.5-7 kb and 13-15 kb. The cell morphology of the new isolates was typical of mycobacteria, electron microscopy revealed a triple-layered cell wall with an irregular electron-dense outer layer. They grew at 10-37 degrees C, with no growth at 45 degrees C in 5 d. The gene encoding the secreted 32 kDa protein, specific to mycobacteria, was detected by PCR. The main whole-cell fatty acids were characterized by high tuberculostearic acid 10Me-C18:0 (17% at 28 degrees C), which increased with increasing growth temperature (22% at 37 degrees C). The other main fatty acids were C18:1 cis9 and C16:0 (21-20% each), followed by, C17:1 cis9 (14%), C16:1 cis10 (8%) and also a high amount of C20 alcohol (9%). alpha-Mycolic acids, keto-mycolates and wax esters were present (C60-C90), MK-9(H2) (90%) and MK-8(H2) were the main menaquinones. The cellular phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidyl inositolmannosides and diphosphatidylglycerol. Polyamine content was low. G+C content was 72.9 mol%. The new isolates are proposed as a new species, Mycobacterium murale sp. nov. The type strain is MA112/96T (= DSM 44340T).  (+info)

Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis. (4/10320)

A novel Treponema species was isolated from an ulcerative lesion of a cow suffering from digital dermatitis (DD), a disease which causes painful ulcerations along the coronary band. Among other anaerobic bacteria, high numbers of spirochaetes have been regularly found in DD lesions. Here data are presented of a spirochaete isolated from a DD ulcer. By chemotaxonomy, protein analysis and comparative 16S rDNA sequence analysis this isolate was classified as a treponeme that differed from all Treponema species described previously. The only isolate, DD5/3T, for which the name Treponema brennaborense is proposed, is designated the type strain of the novel species. The strain is a small, highly motile spirochaete that has two periplasmic flagella, one flagellum being attached at each cell pole. Strain DD5/3T exhibits alpha-glucosidase and N-acetyl-beta-glucosaminidase activity and growth is inhibited by rabbit serum. T. brennaborense was phylogenetically most closely related (89.5% 16S rRNA similarity) to Treponema maltophilum, an oral spirochaete isolated from a periodontitis patient.  (+info)

RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria: a phylogenetic approach. (5/10320)

It has been established that 16S rRNA gene-based phylogeny gives a low resolution between members of the chemoautotrophic ammonia-oxidizing bacteria (AOB) belonging to the beta-subclass of the Proteobacteria. In this study, 12 isolates of AOB were ribotyped, and the sequences of the 16S-23S rDNA intergenic spacer region (ISR) were determined and used in a phylogenetic study. 16S and 23S rDNA ribotyping revealed that the AOB studied contain only one rrn operon per genome, in contrast to most bacteria, which have 5-10 copies of the rRNA genes per genome. It is likely that the presence of only one set of rRNA genes is related to the slow growth of the AOB. The 16S and 23S rRNA genes of the AOB were shown to be arranged in the classical way: a 16S rRNA gene, an ISR and a 23S rRNA gene. Despite the close phylogenetic relationship among the AOB, the relative location of the rRNA genes in the genome appears to vary considerably. The size of the ISR was approximately 400 bp in the Nitrosomonas isolates and 645-694 bp in the Nitrosospira isolates, suggesting a species-specific size difference in the ISR. The ISR contained two potential tRNA genes in the 5' end in all isolates studied. The similarity values between the ISR sequences of the AOB are low (42.9-96.2%) compared with the 16S rDNA sequence similarity values, and therefore the ISR sequences are valuable as a complementary phylogenetic tool in combination with 16S rRNA gene sequences. The phylogenetic analysis of the AOB based on ISR sequences confirms the 16S rRNA gene-based phylogeny but has the benefit of giving a higher resolution.  (+info)

New genus-specific primers for the PCR identification of members of the genera Pseudonocardia and Saccharopolyspora. (6/10320)

Members of the family Pseudonocardiaceae are difficult to identify on the basis of their micromorphology only. The biochemical characterization of each new isolate is a painstaking and time-consuming task which cannot always be undertaken when handling large numbers of strains as is the case in natural product screening programmes. In this study, two sets of genus-specific oligonucleotides were designed which allow rapid detection of members of the genera Pseudonocardia and Saccharopolyspora by means of PCR-specific amplification. The genus specificity of these primers was validated on a wide range of collection strains and the primers were subsequently used to study a group of 106 wild-type isolates that possessed morphological characteristics of the family. Out of this group, 51 strains could be identified as members of the genus Pseudonocardia and only nine isolates could be assigned to the genus Saccharopolyspora. The diversity indicated by whole-cell fatty acid profiles of both wild-type and reference strains was compared with that identified using the oligonucleotide primers. The partial 16S rDNA sequencing of representative wild-type strains was used to validate their genus assignment by PCR-specific amplification. This study shows the industrial usefulness of the application of these direct identification tools as well as the complementary use of two sources of data, PCR-specific amplification results and fatty acid composition, to assess the diversity of a microbial population.  (+info)

Reclassification of Brevibacterium oxydans (Chatelain and Second 1966) as Microbacterium oxydans comb. nov. (7/10320)

Phylogenetic and chemotaxonomic analyses indicate that Brevibacterium oxydans is closely related to species of the genus Microbacterium, namely Microbacterium liquefaciens, Microbacterium luteolum and Microbacterium saperdae. DNA-DNA reassociation values of less than 60% between Brevibacterium oxydans and these three Microbacterium species support the distinctness of this misclassified Brevibacterium species, which is reclassified as Microbacterium oxydans comb. nov.  (+info)

Reclassification of Brevibacterium incertum (Breed 1953) as Desemzia incerta gen. nov., comb. nov. (8/10320)

Phylogenetic analysis of 16S rDNA indicates that Brevibacterium incertum is not a member of the genus Brevibacterium but related to species of the genus Carnobacterium. Hence, Brevibacterium incertum is not a member of the class Actinobacteria but belongs to the phylogenetically defined broad Bacillus-Lactobacillus cluster. Based upon properties that taxonomically clearly distinguishes Brevibacterium incertum from species of the phylogenetic sister genus Carnobacterium, Brevibacterium incertum is reclassified as Desemzia incerta gen. nov., comb. nov.  (+info)

... (rDNA) is a DNA sequence that codes for ribosomal RNA. These sequences regulate transcription initiation and ... Various types of cancers can also be born from mutations of the tandem repeats in the ribosomal DNA. Cell lines can become ... Keil RL, Roeder GS (December 1984). "Cis-acting, recombination-stimulating activity in a fragment of the ribosomal DNA of S. ... Weisburg WG, Barns SM, Pelletier DA, Lane DJ (January 1991). "16S ribosomal DNA amplification for phylogenetic study". Journal ...
Amplified rDNA (Ribosomal DNA) Restriction Analysis is the extension of the technique of RFLP (restriction fragment length ... Identification of Acinetobacter Genomic Species by Amplified Ribosomal DNA Restriction Analysis. J. Clin. Microbiol. 33:11-15. ... Overnight digestion (10-16 hours) of about 300-500 ng of amplicon DNA in a 20 μL system with 4-5 units of Restriction Enzyme ... of more than 4 bp would be irrelevant with respect to a gene of approximately 1500 bp such as that coding for the 16s ribosomal ...
The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res ... 39S ribosomal protein L19, mitochondrial is a protein that in humans is encoded by the MRPL19 gene. Mammalian mitochondrial ... "Entrez Gene: MRPL19 mitochondrial ribosomal protein L19". Nagase T, Miyajima N, Tanaka A, et al. (1995). "Prediction of the ... Graack HR, Bryant ML, O'Brien TW (2000). "Identification of mammalian mitochondrial ribosomal proteins (MRPs) by N-terminal ...
DNA Cell Biol. 15 (12): 1009-1023. doi:10.1089/dna.1996.15.1009. PMID 8985115. Daidone MG, Silvestrini R, Benini E, Grigioni WF ... 40S ribosomal protein SA is a ribosomal protein that in humans is encoded by the RPSA gene. It also acts as a cell surface ... i) Yeast two-hybrid screens have shown that RPSA binds to Ribosomal protein S21 of the 40S small ribosomal subunit. (ii) Serial ... "A region in the C-terminal domain of ribosomal protein SA required for binding of SA to the human 40S ribosomal subunit". ...
DNA Cell Biol. 17 (7): 591-602. doi:10.1089/dna.1998.17.591. PMID 9703018. Lalli E, Ohe K, Hindelang C, Sassone-Corsi P (2000 ... "Entrez Gene: RPL7A ribosomal protein L7a". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... 60S ribosomal protein L7a is a protein that in humans is encoded by the RPL7A gene. Cytoplasmic ribosomes, organelles that ... This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L7AE family of ...
"Primers, 16S ribosomal DNA - François Lutzoni's Lab". lutzonilab.net. Archived from the original on 2012-12-27. Eden PA, ... Weisburg WG, Barns SM, Pelletier DA, Lane DJ (January 1991). "16S ribosomal DNA amplification for phylogenetic study". Journal ... Kolbert CP, Persing DH (June 1999). "Ribosomal DNA sequencing as a tool for identification of bacterial pathogens". Current ... Like the large (23S) ribosomal RNA, it has a structural role, acting as a scaffold defining the positions of the ribosomal ...
"The DNA sequence of human chromosome 22". Nature. 402 (6761): 489-95. Bibcode:1999Natur.402..489D. doi:10.1038/990031. PMID ... "Entrez Gene: RPL3 ribosomal protein L3". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... 60S ribosomal protein L3 is a protein that in humans is encoded by the RPL3 gene. Ribosomes, the complexes that catalyze ... The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV ...
"Entrez Gene: MRPL18 mitochondrial ribosomal protein L18". Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro ... 39S ribosomal protein L18, mitochondrial is a protein that in humans is encoded by the MRPL18 gene. Mammalian mitochondrial ... This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in ... Analysis of the complement of ribosomal proteins present". J Biol Chem. 276 (47): 43958-69. doi:10.1074/jbc.M106510200. PMID ...
RPS3A has been shown to interact with DNA damage-inducible transcript 3. GRCh38: Ensembl release 89: ENSG00000145425 - Ensembl ... 40S ribosomal protein S3a is a protein that in humans is encoded by the RPS3A gene. Ribosomes, the organelles that catalyze ... "Entrez Gene: RPS3A ribosomal protein S3A". Cui, K; Coutts M; Stahl J; Sytkowski A J (March 2000). "Novel interaction between ... This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ...
2003). "Human chromosome 7: DNA sequence and biology". Science. 300 (5620): 767-72. Bibcode:2003Sci...300..767S. doi:10.1126/ ... 39S ribosomal protein L32, mitochondrial is a protein that in humans is encoded by the MRPL32 gene. Mammalian mitochondrial ... This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this ... Analysis of the complement of ribosomal proteins present". J. Biol. Chem. 276 (47): 43958-69. doi:10.1074/jbc.M106510200. PMID ...
2003). "Human chromosome 7: DNA sequence and biology". Science. 300 (5620): 767-72. Bibcode:2003Sci...300..767S. doi:10.1126/ ... "Entrez Gene: RPL21 ribosomal protein L21". Zhou C, Zang D, Jin Y, et al. (March 2011). "Mutation in ribosomal protein L21 ... 60S ribosomal protein L21 is a protein that in humans is encoded by the RPL21 gene. Ribosomes, the organelles that catalyze ... This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ...
Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 ... 60S ribosomal protein L10-like is a protein that in humans is encoded by the RPL10L gene. This gene encodes a protein sharing ... "Entrez Gene: RPL10L ribosomal protein L10-like". Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, ... Overview of all the structural information available in the PDB for UniProt: Q96L21 (Human 60S ribosomal protein L10-like) at ...
Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788-95 ... 60S ribosomal protein L36 is a protein that in humans is encoded by the RPL36 gene. Ribosomes, the organelles that catalyze ... "Entrez Gene: RPL36 ribosomal protein L36". Overview of all the structural information available in the PDB for UniProt: Q9Y3U8 ... This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L36E family of ...
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788-95 ... 39S ribosomal protein L15, mitochondrial is a protein that in humans is encoded by the MRPL15 gene. Mammalian mitochondrial ... This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this ... 2000). "Mammalian mitochondrial ribosomal proteins (2). Amino acid sequencing, characterization, and identification of ...
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788-95 ... 60S ribosomal protein L10 is a protein that in humans is encoded by the RPL10 gene. Ribosomes, the organelles that catalyze ... "Entrez Gene: RPL10 ribosomal protein L10". Oh HS, Kwon H, Sun SK, Yang CH (September 2002). "QM, a putative tumor suppressor, ... Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (Aug 1998). "A map of 75 human ribosomal ...
Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788-95 ... "Entrez Gene: RPS24 ribosomal protein S24". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... 40S ribosomal protein S24 is a protein that in humans is encoded by the RPS24 gene. Ribosomes, the organelles that catalyze ... Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (Aug 1998). "A map of 75 human ribosomal ...
2004). "DNA sequence and analysis of human chromosome 9". Nature. 429 (6990): 369-74. Bibcode:2004Natur.429..369H. doi:10.1038/ ... 39S ribosomal protein L41, mitochondrial is a protein that in humans is encoded by the MRPL41 gene. Mammalian mitochondrial ... 2005). "Mitochondrial ribosomal protein L41 suppresses cell growth in association with p53 and p27Kip1". Mol. Cell. Biol. 25 ( ... 2006). "Mitochondrial ribosomal protein L41 mediates serum starvation-induced cell-cycle arrest through an increase of p21(WAF1 ...
2004). "DNA sequence and analysis of human chromosome 9". Nature. 429 (6990): 369-74. Bibcode:2004Natur.429..369H. doi:10.1038/ ... 60S ribosomal protein L35 is a protein that in humans is encoded by the RPL35 gene. Ribosomes, the organelles that catalyze ... "Entrez Gene: RPL35 ribosomal protein L35". Human RPL35 genome location and RPL35 gene details page in the UCSC Genome Browser. ... This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ...
2005). "The DNA sequence of the human X chromosome". Nature. 434 (7031): 325-37. Bibcode:2005Natur.434..325R. doi:10.1038/ ... "Entrez Gene: RPL36A ribosomal protein L36a". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... Although this gene has been referred to as ribosomal protein L44 (RPL44), its official name is ribosomal protein L36a (RPL36A ... The protein, which shares sequence similarity with yeast ribosomal protein L44, belongs to the L44E (L36AE) family of ribosomal ...
Cross SH, Charlton JA, Nan X, Bird AP (1994). "Purification of CpG islands using a methylated DNA binding column". Nat. Genet. ... "Entrez Gene: RPL24 ribosomal protein L24". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed ... 60S ribosomal protein L24 is a protein that in humans is encoded by the RPL24 gene. Ribosomes, the organelles that catalyze ...
2004). "The DNA sequence and biology of human chromosome 19". Nature. 428 (6982): 529-35. Bibcode:2004Natur.428..529G. doi: ... "Entrez Gene: RPL18A ribosomal protein L18a". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... 60S ribosomal protein L18a is a protein that in humans is encoded by the RPL18A gene. Ribosomes, the organelles that catalyze ... Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (Aug 1998). "A map of 75 human ribosomal ...
2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805-11. Bibcode:2003Natur.425..805M. doi: ... "Entrez Gene: RPL10A ribosomal protein L10a". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this ... 60S ribosomal protein L10a is a protein that in humans is encoded by the RPL10A gene. Ribosomes, the organelles that catalyze ...
Cross SH, Charlton JA, Nan X, Bird AP (March 1994). "Purification of CpG islands using a methylated DNA binding column". Nature ... "Entrez Gene: RPL32 ribosomal protein L32". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... 60S ribosomal protein L32 is a protein that in humans is encoded by the RPL32 gene. Ribosomes, the organelles that catalyze ... This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ...
2005). "Myc represses transcription through recruitment of DNA methyltransferase corepressor". EMBO J. 24 (2): 336-46. doi: ... "Entrez Gene: RPS8 ribosomal protein S8". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... 40S ribosomal protein S8 is a protein that in humans is encoded by the RPS8 gene. Ribosomes, the organelles that catalyze ... 1991). "Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ...
2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805-11. Bibcode:2003Natur.425..805M. doi: ... "Entrez Gene: RPS18 ribosomal protein S18". Wool IG, Chan YL, Glück A (1996). "Structure and evolution of mammalian ribosomal ... 40S ribosomal protein S18 is a protein that in humans is encoded by the RPS18 gene. Ribosomes, the organelles that catalyze ... The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ...
Cross SH, Charlton JA, Nan X, Bird AP (1994). "Purification of CpG islands using a methylated DNA binding column". Nat. Genet. ... 60S ribosomal protein L40 (RPL40) is a protein that in humans is encoded by the UBA52 gene. Ubiquitin is a highly conserved ... This gene encodes a fusion protein consisting of ubiquitin at the N-terminus and ribosomal protein L40 at the C-terminus, a C- ... 2002). "The human ribosomal protein genes: sequencing and comparative analysis of 73 genes". Genome Res. 12 (3): 379-90. doi: ...
2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315-21. Bibcode:2006Natur.441.. ... 39S ribosomal protein L24, mitochondrial is a protein that in humans is encoded by the MRPL24 gene. Mammalian mitochondrial ... "Entrez Gene: MRPL24 mitochondrial ribosomal protein L24". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to ... 2000). "Mammalian mitochondrial ribosomal proteins (4). Amino acid sequencing, characterization, and identification of ...
"Entrez Gene: MRPL37 mitochondrial ribosomal protein L37". Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and ... 39S ribosomal protein L37, mitochondrial is a protein that in humans is encoded by the MRPL37 gene. Mammalian mitochondrial ... Overview of all the structural information available in the PDB for UniProt: Q9BZE1 (39S ribosomal protein L37, mitochondrial) ... Graack HR, Bryant ML, O'Brien TW (Jan 2000). "Identification of mammalian mitochondrial ribosomal proteins (MRPs) by N-terminal ...
2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315-21. Bibcode:2006Natur.441.. ... 39S ribosomal protein L20, mitochondrial is a protein that in humans is encoded by the MRPL20 gene. Mammalian mitochondrial ... "Entrez Gene: MRPL20 mitochondrial ribosomal protein L20". Venter JC, Adams MD, Myers EW, et al. (2001). "The sequence of the ... Analysis of the complement of ribosomal proteins present". J. Biol. Chem. 276 (47): 43958-69. doi:10.1074/jbc.M106510200. PMID ...
https://www.sciencedirect.com/science/article/pii/S1876162323000330) Keywords: DNA replication; DNA replication stress; Genome ... Ribosomal protein; p53; MDM2; p14-ARF Abdol-Hossein Rezaeian, Hiroyuki Inuzuka, Wenyi Wei, Chapter Six - Insights into the ... Chromatin regulators in DNA replication and genome stability maintenance during S-phase, Editor(s): Rossen Donev, Advances in ... Ribosomal protein inhibiting CDKs; Therapeutics Ashna Gupta, Gunjan Dagar, Ravi Chauhan, Hana Q. Sadida, Sara K. Almarzooqi, ...
Ribosomal DNA (rDNA) is a DNA sequence that codes for ribosomal RNA. These sequences regulate transcription initiation and ... Various types of cancers can also be born from mutations of the tandem repeats in the ribosomal DNA. Cell lines can become ... Keil RL, Roeder GS (December 1984). "Cis-acting, recombination-stimulating activity in a fragment of the ribosomal DNA of S. ... Weisburg WG, Barns SM, Pelletier DA, Lane DJ (January 1991). "16S ribosomal DNA amplification for phylogenetic study". Journal ...
Antibodies / Assay Kits / Biology Cells / cDNA / Clia Kits / Culture Cells / Devices / DNA / DNA Templates / DNA Testing / ... Copyright © 2023 Primers, 16s Ribosomal DNA - François Lutzonis Lab - OnePress theme by FameThemes ...
Copyright © 2023 Primers, 16s Ribosomal DNA - François Lutzonis Lab - OnePress theme by FameThemes ... Antibodies / Assay Kits / Biology Cells / cDNA / Clia Kits / Culture Cells / Devices / DNA / DNA Templates / DNA Testing / ... Antibodies / Assay Kits / Biology Cells / cDNA / Clia Kits / Culture Cells / Devices / DNA / DNA Templates / DNA Testing / ... Antibodies / Assay Kits / Biology Cells / cDNA / Clia Kits / Culture Cells / Devices / DNA / DNA Templates / DNA Testing / ...
Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal ... Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal ... Intact ribosomal DNA arrays of Potentilla origin detected in Erythronium nucleus suggest recent eudicot-to-monocot horizontal ... This raised the possibility of horizontal transfer of a eudicot alien ribosomal DNA (rDNA) into the Erythronium genome. In this ...
Seasonal Succession Leads to Habitat-Dependent Differentiation in Ribosomal RNA:DNA Ratios among Freshwater Lake Bacteria. ... However, inconsistent correlations between ribosomal RNA:DNA ratios and metabolic activity or growth rates have led to a more ... These differences, made apparent when calculating ribosomal RNA:DNA ratios, have been interpreted as variable activities of ... Seasonal Succession Leads to Habitat-Dependent Differentiation in Ribosomal RNA:DNA Ratios among Freshwater Lake Bacteria ...
Ribosomal DNA variation and population differentiation in Salicornia L. In: New Phytologist. 1992 ; Vol. 122, No. 3. pp. 553- ... Ribosomal DNA variation and population differentiation in Salicornia L. / Noble, SM; Davy, AJ; Oliver, RP. ... Noble, SM, Davy, AJ & Oliver, RP 1992, Ribosomal DNA variation and population differentiation in Salicornia L., New ... title = "Ribosomal DNA variation and population differentiation in Salicornia L.",. abstract = "Analysis of nuclear rDNA ...
The precursor of mature 18S, 28S and 5.8S ribosomal RNAs is transcribed from the ribosomal DNA gene (rDNA), which exists as 300 ... We found that rDNA locus tended to be hypermethylated in tumor compared to matched normal breast tissues and that the DNA ... From: The nucleolar size is associated to the methylation status of ribosomal DNA in breast carcinomas ...
Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA ... Properties and Composition of the Isolated Ribosomal DNA Satellite of Xenopus laevis. scientific article published in Nature ... Properties and composition of the isolated ribosomal DNA satellite of Xenopus laevis (English) ... Properties and Composition of the Isolated Ribosomal DNA Satellite of Xenopus laevis (English) ...
S7 nuclear ribosomal DNA. The S7 nuclear region was represented by 127 unique alleles from a total of 274 sequences (138 ... Mitochondrial DNA control region. A 345-bp segment of the mtDNA control region was obtained from 227 individuals, yielding 116 ... Chow S, Hazama K . (1998). Universal PCR primers for S7 ribosomal protein gene introns in fish. Mol Ecol 7: 1255-1256. ... Haplotype relationships of D. vulgaris represented in a median joining network for mtDNA (CR) and nuclear DNA (S7). Haplotypes ...
DNA. , Ribosomal. Spacer/genetics ; DNA. , Ribosomal. Spacer/chemistry ; DNA. , Helminth/genetics ; Sheep, Domestic/genetics ; ... DNA. , Ribosomal. *. Arthrodermataceae/*genetics. Arthrodermataceae/isolation & purification ; DNA. , Fungal ; DNA. , Ribosomal ... DNA. , Ribosomal. */genetics DNA. , Ribosomal. */metabolism Genome*. Animals ; Humans ; Mice ; Chromatin ; Chromosome Mapping ... DNA. Copy Number Variations* Autism Spectrum Disorder*/genetics. Humans ; DNA. , Ribosomal. /genetics ; DNA. Methylation ; ...
DNA-DNA Reassociation , definition of DNA-DNA Reassociation by Medical dictionary. Looking for online definition of DNA-DNA ... What is DNA-DNA Reassociation? Meaning of DNA-DNA Reassociation medical term. What does DNA-DNA Reassociation mean? ... Diversity and Inheritance of Intergenic Spacer Sequences of 45S Ribosomal DNA among Accessions of.... Ribosomal DNA (rDNA) of ... Genomic DNA was extracted from the four species of Tilapia. About 2000 bp 18S ribosomal DNA was amplified by PCR using specific ...
53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling ... 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling ...
Ribosomal DNA genetic variation and phenotypes 04 October 2023. Seminar with Michelle Holland ...
RNA polymerase II has an unexpected function in the nucleolus, helping to drive the expression of ribosomal RNA and to protect ... are the only enzymes that directly mediate the expression of the ribosomal RNA (rRNA) components of ribosomes. Here we show, ... The DNA was then purified using gel/PCR DNA fragment extraction (Geneaid, catalogue number DF300) and qPCR of purified DNA was ... DNA-RNA hybrid immunoprecipitation. For DNA-RNA hybrid immunoprecipitation (DRIP) experiments, cells were first seeded in 60 mm ...
... in the largest biology dictionary online. Free learning resources for students covering all major areas of ... DNA sequence that codes for ribosomal RNA. Supplement. Ribosomal DNA is the sequence of DNA that codes for ribosomal RNA. In ... 1 Ribosomal DNA. Retrieved from https://en.wikipedia.org/wiki/Ribosomal-DNA. ... Ribosomal RNA is a specialized RNA molecule present in the ribosome. The ribosomal RNA does not carry the code in making ...
Ribosomal DNA (16S rDNA) sequencing answers are found in the Guide to Diagnostic Tests powered by Unbound Medicine. Available ... "Ribosomal DNA (16S rDNA) Sequencing." Guide to Diagnostic Tests, 7th ed., McGraw-Hill Education, 2017. Emergency Central, ... Ribosomal DNA (16S rDNA) sequencing for organism identification Collect and freeze (-70°C) unidentifiable culture isolate, ... Ribosomal DNA (16S rDNA) Sequencing [Internet]. In: Guide to Diagnostic Tests. McGraw-Hill Education; 2017. [cited 2023 ...
Kazusa DNA Research Institute. * Kazusa DNA Research Institute *NITE Biological Resource Center ... large ribosomal subunit protein mL38. Names. 39S ribosomal protein L38, mitochondrial. mitochondrial large ribosomal subunit ... MRPL38 mitochondrial ribosomal protein L38 [Homo sapiens] MRPL38 mitochondrial ribosomal protein L38 [Homo sapiens]. Gene ID: ... mitochondrial ribosomal protein L38provided by HGNC. Primary source. HGNC:HGNC:14033 See related. Ensembl:ENSG00000204316 MIM: ...
Kazusa DNA Research Institute. * Kazusa DNA Research Institute *LifeSpan BioSciences, Inc.. * LSBio RPS16 / Ribosomal Protein ... PLN00210; 40S ribosomal protein S16; Provisional. * NM_001321111.2 → NP_001308040.1 small ribosomal subunit protein uS9 isoform ... PLN00210; 40S ribosomal protein S16; Provisional. * NM_001363860.2 → NP_001350789.1 small ribosomal subunit protein uS9 isoform ... RPS16 ribosomal protein S16 [Homo sapiens] RPS16 ribosomal protein S16 [Homo sapiens]. Gene ID:6217 ...
The use of mitichondrial ribosomal DNA for algal and protistan phylogenetic analysis. Saved in: ... A study of the effect of taxol on DNA and RNA synthesis in murine erythroleukemia cells. by: Whelchel, Beth Carly. Published: ( ... study of the evolution of mouthbrooding in Cichlids by statistical analysis of the cytochrome b gene found in mitochondrial DNA ...
... Auteurs. Coulon S, Noguchi E, Noguchi ... Rad22Rad52-dependent repair of ribosomal DNA repeats cleaved by Slx1-Slx4 endonuclease.. ... The increased fork pausing at the replication fork barriers in the rDNA repeats in a strain that lacks Rqh1 DNA helicase is ... events or process recombination structures that occur during the replication of the tandem repeats of the ribosomal DNA (rDNA) ...
Ribosomal RNA (rRNA) and transfer RNA (tRNA). Ribosomal RNA (rRNA) is a major component of ribosomes, where it helps mRNA bind ... Just keep in mind that, even though all life forms have DNA, not everything that has DNA is alive: viruses can have DNA but are ... DNA in cells. In eukaryotes, such as plants and animals, DNA is found in the nucleus, a specialized, membrane-bound vault in ... DNA and RNA are polymers (in the case of DNA, often very long polymers), and are made up of monomers known as nucleotides. When ...
Molecular organization evolution function of ribosomal DNA. epigenetics molecular phylogeny and taxonomy nucleolus polyploidy ... Editorial: Molecular organization, evolution, and function of ribosomal DNA. Frontiers in Plant Science, 2022, 13, ⟨10.3389/ ...
DNA, Ribosomal / genetics * Molecular Sequence Data * Phylogeny* * Species Specificity Substances * DNA, Ribosomal ...
Dive into the research topics of Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction- ... T1 - Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length ... title = "Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length ... Differentiation of oral Actinomyces species by 16S ribosomal DNA polymerase chain reaction-restriction fragment length ...
... we revealed differences in the location and size of C-heterochromatin blocks and ribosomal DNA clusters. A comparative analysis ... clusters of ribosomal DNA and telomeric repeats of 10 species of the family Pamphagidae from Morocco are described for the ... but to the evolution of repetitive DNA. ...
DNA, RNA, cDNA, mRNA, tRNA, or rDNA (Note: c, complementary; m, messenger; t, transfer; r, ribosomal) ... RNA, DNA, cDNA, mRNA, tRNA, or rDNA (Note: c, complementary; m, messenger; t, transfer; r, ribosomal) ... DNA, RT-PCR). For the purposes of this section, "abbreviation" will refer to all of these. ...
RNA, Ribosomal, 16S / genetics* * Vasculitis Substances * DNA, Ribosomal * Drug Combinations * Glutamates * RNA, Ribosomal, 16S ...
  • In Bacteria, Archaea, and chloroplasts the rRNA is composed of different (smaller) units, the large (23S) ribosomal RNA, 16S ribosomal RNA and 5S rRNA. (wikipedia.org)
  • HOT1 includes an RNA polymerase I (PolI) transcription promoter that catalyzes 35S ribosomal rRNA gene transcription. (wikipedia.org)
  • Downregulation of ribosomal RNA (rRNA) genes in human head and neck squamous cell carcinoma (HNSCC) cells correlates with rDNA promoter hypermethylation. (wroc.pl)
  • Existing models suggest that RNA polymerases I and III (Pol I and Pol III) are the only enzymes that directly mediate the expression of the ribosomal RNA (rRNA) components of ribosomes. (nature.com)
  • 16S ribosomal RNA (rRNA) gene sequencing is the current gold standard in bacterial and other pathogenic microorganism identification and classification. (unboundmedicine.com)
  • Binding of human ribosomal protein S16 with the 18S rRNA fragment 1203-1236/1521-1698]. (nih.gov)
  • The 16S rRNA gene sequences from isolated genomic DNA samples were amplified by PCR. (elsevierpure.com)
  • c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. (fhcrc.org)
  • Grandori, Carla and Gomez-Roman, Natividad and Felton-Edkins, Zoe A and Ngouenet, Celine and Galloway, Denise A and Eisenman, Robert N and White, Robert J (2005) c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I. Nature cell biology, 7 (3). (fhcrc.org)
  • Here, we demonstrate that human c-Myc also directly enhances Pol I transcription of ribosomal RNA (rRNA) genes. (fhcrc.org)
  • Furthermore, by using combined immunofluorescence and rRNA-FISH, we have detected endogenous c-Myc in nucleoli at sites of active ribosomal DNA (rDNA) transcription. (fhcrc.org)
  • BackgroundGenetic markers like the nuclear ribosomal RNA (rRNA) genes, internal transcribed spacer regions, mitochondrial protein-coding genes, and genomes have been utilized for molecular identification of parasitic trematodes. (ox.ac.uk)
  • The convenience and applicability of the newly designed primers and adequate genetic variation of the mitochondrial rRNA genes can be useful as complementary markers for trematode molecular-based studies.ConclusionsWe demonstrated that the mitochondrial rRNA genes could be alternative genetic markers robust for trematode molecular identification and potentially helpful for DNA barcoding where our primers can be widely applied across the major Digenea orders. (ox.ac.uk)
  • Application of the 5' fluorogenic exonuclease assay (TaqMan) for quantitative ribosomal DNA and rRNA analysis in sediments. (umass.edu)
  • Assay accuracy was significantly improved by employing a system of replicate dilutions and replicate analyses for both DNA and rRNA quantitation. (umass.edu)
  • In a subset of 10 representative patients, bacterial DNA was extracted from ascites and whole blood, followed by 16S rRNA gene amplicon sequencing. (frontiersin.org)
  • Relative abundance profiles of bacterial populations measured by sequencing DNA or RNA of marker genes can widely differ. (doe.gov)
  • The bacterial genes encoding chloramphenicol acetyltransferase (CAT) and beta-glucuronidase (GUS) were transiently expressed in leaf discs from the AR1 promoter in TGMV A. The levels of AR1 and GUS RNAs were similar in leaf discs after adjusting for viral DNA copy number, while CAT RNA was less abundant. (ncsu.edu)
  • Chromatin structure and expression of plant ribosomal RNA genes. (ncsu.edu)
  • Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. (nih.gov)
  • The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders. (nih.gov)
  • As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (nih.gov)
  • For instance, some genes specify ribosomal RNAs ( rRNAs ), which serve as structural components of ribosomes, or transfer RNAs ( tRNAs ), cloverleaf-shaped RNA molecules that bring amino acids to the ribosome for protein synthesis. (khanacademy.org)
  • This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
  • The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes. (ox.ac.uk)
  • Methods: DNAs from 92 oral washings from 52 participants were subject to metabarcoding of ribosomal genes. (lu.se)
  • Ribosomal DNA (rDNA) is a DNA sequence that codes for ribosomal RNA. (wikipedia.org)
  • The rDNA repeats have many regulatory mechanisms that keep the DNA from undergoing mutations, thus keeping the rDNA conserved. (wikipedia.org)
  • A fragment of yeast rDNA containing the 5S gene, non-transcribed spacer DNA, and part of the 35S gene has localized cis-acting mitotic recombination stimulating activity. (wikipedia.org)
  • This raised the possibility of horizontal transfer of a eudicot alien ribosomal DNA (rDNA) into the Erythronium genome. (cas.cz)
  • Emergency Central , emergency.unboundmedicine.com/emergency/view/GDT/619539/all/Ribosomal_DNA__16S_rDNA__sequencing. (unboundmedicine.com)
  • It is thought to initiate recombination events or process recombination structures that occur during the replication of the tandem repeats of the ribosomal DNA (rDNA) locus. (crcm-marseille.fr)
  • The increased fork pausing at the replication fork barriers in the rDNA repeats in a strain that lacks Rqh1 DNA helicase is further increased by expression of a dominant negative form of Slx1. (crcm-marseille.fr)
  • The identification of species was addressed through a combination of biochemical and genetic methods (API ® 50 CHL test, 16S rDNA and recA gene sequences, Amplified Ribosomal DNA Restriction Analysis -ARDRA- and 16S-26S intergenic region analysis). (mdpi.com)
  • During our initial phylogenetic study of the monocot genus Erythronium (Liliaceae), we observed peculiar eudicot-type internal transcribed spacer (ITS) sequences in a dataset derived from genomic DNA of Erythronium dens-canis. (cas.cz)
  • Alignment of sequences was performed for the excised mitochondrial 12S ribosomal DNA sequences, and conserved regions were identified for all 64 mitochondrial genomes. (lookformedical.com)
  • This type of RNA is called a messenger RNA ( mRNA ), as it serves as a messenger between DNA and the ribosomes, molecular machines that read mRNA sequences and use them to build proteins. (khanacademy.org)
  • The nuclear ribosomal DNA internal transcribed spacer (ITS) sequences from 44 Indian Polygonum taxa were examined to investigate relationships among various sections proposed by earlier researchers. (geneticsmr.com)
  • Most of these sequences qualify as junk but they are littered with defective transposable elements that are already included in the calculation of junk DNA. (blogspot.com)
  • DNA sequences [ 4-6 ]. (who.int)
  • DNA sequences were identified to genus level and submitted to oral microbiota diversity analyses. (lu.se)
  • In this study we designed a set of PCR primers for the mitochondrial 12S ribosomal DNA sequence based on 64 complete mitochondrial genomes and then tested their efficacy. (lookformedical.com)
  • These primers amplify approximately 600 basepairs of the ITS1-5.8S-ITS2 region of the ribosomal cistron. (cdc.gov)
  • Although the ITS primers are universal for fungi, the D1D2 region of the large ribosomal subunit has better discrimination for yeasts, with primers NL-1 and NL-4. (cdc.gov)
  • These primers amplify approximately 620 basepairs of the 28S region of the ribosomal cistron. (cdc.gov)
  • These primers amplify a section of the intergenic spacer in the ribosomal cistron. (cdc.gov)
  • The nuclear ribosomal repeats for the 18S, 5.8S, and 26S RNAs of two closely related Picea (spruce) species were characterized by restriction mapping and Southern blot hybridization. (lookformedical.com)
  • The ribosomal RNAs form two subunits, i.e. large subunit and small subunit. (biologyonline.com)
  • For identification of Trichosporon species, the intergenic region of the ribosomal cistron is used (Sugita, 2002). (cdc.gov)
  • Nucleotide sequence variation in the internal transcribed spacer (ITS) region of the ribosomal gene was investigated in 28 Medicago species,and Metilotus officinalis was included as an outgroup. (usda.gov)
  • Five major nuclear ribosomal repeats represent a large and variable fr by Michael S. Bobola, Dirk E. Smith et al. (lookformedical.com)
  • Rad22Rad52-dependent repair of ribosomal DNA repeats cleaved by Slx1-Slx4 endonuclease. (crcm-marseille.fr)
  • Studying ribosomal DNA repeats in newly assembled genomes. (mpg.de)
  • This study adds a new example to the rarely documented nuclear-to-nuclear jumps of DNA between eudicots and monocots while holding the scientific community continually in suspense about the mode of DNA transfer. (cas.cz)
  • Purification and restriction endonuclease analysis of plant nuclear DNA. (ncsu.edu)
  • This study represents phylogenetic analyses of two woody polygonaceous genera Calligonum and Pteropyrum using both chloroplast fragment (trnL-F) and the nuclear ribosomal internal transcribed spacer (nrDNA ITS) sequence data. (ijbiotech.com)
  • This gene encodes a ribosomal protein that is a component of the 40S subunit. (nih.gov)
  • The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
  • To facilitate identification of R. mannitolilytica and R. pickettii , we developed 16S ribosomal DNA-based polymerase chain reaction assays that allow sensitive and specific identification of these species. (cdc.gov)
  • Mycobacterial DNA was not detected with quantitative polymerase chain reaction. (cdc.gov)
  • Human ribosomal protein S16 inhibites excision of the first intron from its own]. (nih.gov)
  • Ribosomal RNA is a specialized RNA molecule present in the ribosome. (biologyonline.com)
  • Cytosine methylation in ribosomal DNA and nucleolus organiser expression in wheat. (ncsu.edu)
  • The ribosomal RNA does not carry the code in making proteins. (biologyonline.com)
  • The protein belongs to the S9P family of ribosomal proteins. (nih.gov)
  • MicroRNA-7641 is a regulator of ribosomal proteins and a promising targeting factor to improve the efficacy of cancer therapy. (nih.gov)
  • DNA bears the hereditary information that's passed on from parents to children, providing instructions for how (and when) to make the many proteins needed to build and maintain functioning cells, tissues, and organisms. (khanacademy.org)
  • Investigation of the DNA Maintenance Methylation Machinery. (mpg.de)
  • Characterization of tools for the global and targeted manipulation of DNA methylation. (mpg.de)
  • They could occur by slippage of the newly synthesized strand during DNA replication or by gene conversion. (wikipedia.org)
  • The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component contains the only virally-encoded function required for autonomous replication in infected plant cells. (ncsu.edu)
  • Synthesis of AR1 RNA was dependent on T-DNA transfer and TGMV DNA replication, demonstrating that it is a plant transcription product. (ncsu.edu)
  • Also included are topics on DNA replication during interphase of the cell cycle, DNA mutation and repair mechanisms, gene pool, modification, and diseases. (biologyonline.com)
  • Recent advances in genomic DNA sequencing of microbial species from single cells. (unboundmedicine.com)
  • DNA extraction, amplification, and sequencing: Total genomic DNA was isolated from dried leaf or branchlet (only for Calligonum) of samples using the modified cetyl trimethylammonium bromide (CTAB) method of Doyle and Doyle (1987). (ijbiotech.com)
  • Construction and validation of customized genomes for human and mouse ribosomal DNA mapping. (wroc.pl)
  • Like mammalian genomes, the sea urchin genome contains a lot of junk DNA, especially repetitive DNA. (blogspot.com)
  • Mature rRNAs are packaged into 40S and 60S ribosomal subunits for export to the cytoplasm. (nature.com)
  • Slx1 and Slx4 are subunits of a structure-specific DNA endonuclease that is found in Saccharomyces cerevisiae, Schizosaccharomyces pombe, and other eukaryotic species. (crcm-marseille.fr)
  • deoxyribonucleic acid ( DNA ) and ribonucleic acid ( RNA ). (khanacademy.org)
  • Ribosomal DNA variation and population differentiation in Salicornia L. (uea.ac.uk)
  • 1992). Ribosomal DNA variation and population differentiation in Salicornia L. New Phytologist , 122 (3), 553-565. (uea.ac.uk)
  • As a result, this DNA can provide phylogenetic information of species belonging to wide systematic levels. (wikipedia.org)
  • Trichophyton species DNA is amplified very poorly by the ITS primer set used for most other molds. (cdc.gov)
  • Ribosomal RNA analyses demonstrated presence of additional bacterial and fungal species not identified with culture. (cdc.gov)
  • The identification of the ribosomal RNA cistron by sequence complementarity. (wikidata.org)
  • Holdings: The use of mitichondrial ribosomal DNA for algal and protistan phylogenetic analysis. (lafayette.edu)
  • The use of mitichondrial ribosomal DNA for algal and protistan phylogenetic analysis. (lafayette.edu)
  • The ratio between AR1 RNA and the major leftward RNA was constant and was used to normalize AR1 transcription for viral DNA copy number. (ncsu.edu)
  • do not use an en dash between reverse transcription and PCR. (cdc.gov)
  • use a hyphen in the term real-time, but do not use an en dash between reverse transcription and PCR. (cdc.gov)
  • Bases include the pyrimidine bases (cytosine, thymine in DNA, and uracil in RNA, one ring) and the purine bases (adenine and guanine, two rings). (khanacademy.org)
  • Each nucleotide in DNA contains one of four possible nitrogenous bases: adenine (A), guanine (G) cytosine (C), and thymine (T). Adenine and guanine are purines , meaning that their structures contain two fused carbon-nitrogen rings. (khanacademy.org)
  • A study of the effect of taxol on DNA and RNA synthesis in murine erythroleukemia cells. (lafayette.edu)
  • DNA and RNA are polymers (in the case of DNA, often very long polymers), and are made up of monomers known as nucleotides . (khanacademy.org)
  • Variations in ribosomal DNA copy numbers in a genome of Trichophyton interdigitale. (wroc.pl)
  • Vol 63: Ribosomal DNA Sequence Heterogeneity Reflects Intraspecies Phylogenies and Predicts Genome Structure in Two Contrasting. (lookformedical.com)
  • In 1972 the late geneticist Susumu Ohno coined the term "junk DNA" to describe all noncoding sections of a genome, most of which consist of repeated segments scattered randomly throughout the genome. (blogspot.com)
  • How DNA carries this information, and how it is put into action by cells and organisms, is complex, fascinating, and fairly mind-blowing, and we'll explore it in more detail in the section on molecular biology . (khanacademy.org)
  • This progression from DNA to RNA to protein is called the " central dogma " of molecular biology. (khanacademy.org)
  • These differences, made apparent when calculating ribosomal RNA:DNA ratios, have been interpreted as variable activities of bacterial populations. (doe.gov)
  • Detection of bacterial DNA has been investigated as a marker of SBP and as a predictor of prognosis. (frontiersin.org)
  • Here we investigated whether levels of IL-6 and IL-8 putatively produced by myeloid cells in ascites are associated with systemic inflammation and whether inflammation depends on the presence of specific bacterial DNA. (frontiersin.org)
  • In eukaryotes, DNA is typically broken up into a number of very long, linear pieces called chromosomes , while in prokaryotes such as bacteria, chromosomes are much smaller and often circular (ring-shaped). (khanacademy.org)
  • Parallel samples from 51 chronic wounds were studied using aerobic culturing and 16S DNA sequencing for the identification of bacteria. (lookformedical.com)
  • DNA is the genetic material found in living organisms, all the way from single-celled bacteria to multicellular mammals like you and me. (khanacademy.org)
  • In prokaryotes, such as bacteria, the DNA is not enclosed in a membranous envelope, although it's located in a specialized cell region called the nucleoid . (khanacademy.org)
  • This DNA fragment contains a mitotic recombination hotspot, referred to as HOT1. (wikipedia.org)
  • A chloroplast DNA restriction fragment length polymorphism (RFLP) and its relationship with cone morphology was studied in a sympatric population of lodgepole pine and jack pine. (researchgate.net)
  • Instead, a molecule called a noncoding RNA, a chemical cousin of DNA, is produced from the RMRP gene. (medlineplus.gov)
  • A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
  • RESUME Une PCR (amplification en chaîne par polymérase) diagnostique avec des amorces spécifiques d'espèces et un séquençage direct ont été utilisés pour la détermination des membres du complexe Ano- pheles maculipennis dans les régions du nord-ouest et du centre de la République islamique d'Iran. (who.int)
  • an initialism is an acronym that is pronounced as individual letters (DNA, RT-PCR). (cdc.gov)
  • We have identified a chloroplast DNA polymorphism that separates shortleaf, slash, longleaf, and loblolly pines into three groups. (researchgate.net)
  • Chloroplast DNA typical of jack pine. (researchgate.net)
  • it does not describe the procedure for purification of fungal DNA. (cdc.gov)
  • Unlike microbial studies, where analysis of the 16S ribosomal DNA sequence is standard, the best gene for metazoan metagenetics is less clear. (lookformedical.com)
  • The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)
  • During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). (cdc.gov)