Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
DNA probes specific for the human leukocyte antigen genes, which represent the major histocompatibility determinants in humans. The four known loci are designated as A, B, C, and D. Specific antigens are identified by a locus notation and number, e.g., HLA-A11. The inheritance of certain HLA alleles is associated with increased risk for certain diseases (e.g., insulin-dependent diabetes mellitus).
Deoxyribonucleic acid that makes up the genetic material of bacteria.
3 beta,12 beta,14-Trihydroxy-5 beta-card-20(22)-enolide. A cardenolide which is the aglycon of digoxin. Can be obtained by hydrolysis of digoxin or from Digitalis orientalis L. and Digitalis lanata Ehrh.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
DNA probes specific for the identification of human papilloma virus.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The functional hereditary units of BACTERIA.
A tick-borne septicemic disease of domestic and wild ruminants caused by EHRLICHIA RUMINANTIUM.
Any method used for determining the location of and relative distances between genes on a chromosome.
Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Deoxyribonucleic acid that makes up the genetic material of viruses.
The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A species of gram-negative bacteria in the family ANAPLASMATACEAE, that causes HEARTWATER DISEASE in ruminants.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
The male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans and in some other male-heterogametic species in which the homologue of the X chromosome has been retained.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Mapping of the KARYOTYPE of a cell.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.
A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It is closely related to SHIGA TOXIN produced by SHIGELLA DYSENTERIAE.
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Techniques used in studying bacteria.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Actual loss of portion of a chromosome.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Abnormal number or structure of the SEX CHROMOSOMES. Some sex chromosome aberrations are associated with SEX CHROMOSOME DISORDERS and SEX CHROMOSOME DISORDERS OF SEX DEVELOPMENT.
The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.
Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
Measurement of the intensity and quality of fluorescence.
A genus of bacteria found in the reproductive organs, intestinal tract, and oral cavity of animals and man. Some species are pathogenic.
Established cell cultures that have the potential to propagate indefinitely.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A complex that includes several strains of M. avium. M. intracellulare is not easily distinguished from M. avium and therefore is included in the complex. These organisms are most frequently found in pulmonary secretions from persons with a tuberculous-like mycobacteriosis. Strains of this complex have also been associated with childhood lymphadenitis and AIDS; M. avium alone causes tuberculosis in a variety of birds and other animals, including pigs.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4.
Uracil nucleotides which contain deoxyribose as the sugar moiety.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.
A species of gram-negative bacteria in the genus PORPHYROMONAS, family Porphyromonadaceae. It is a key pathogen in endodontic infections.
So-called atypical species of the genus MYCOBACTERIUM that do not cause tuberculosis. They are also called tuberculoid bacilli, i.e.: M. buruli, M. chelonae, M. duvalii, M. flavescens, M. fortuitum, M. gilvum, M. gordonae, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. kansasii, M. marinum, M. obuense, M. scrofulaceum, M. szulgai, M. terrae, M. ulcerans, M. xenopi.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
A foul-smelling accumulation of SEBUM and desquaminated epidermal cells, especially the cheesy substance found under the foreskin of the penis and at the base of the labia minor near the clitoris.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence G/AATTC at the slash. EcoRI is from E coliRY13. Several isoschizomers have been identified. EC 3.1.21.-.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Infections with bacteria of the species ESCHERICHIA COLI.
A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.
The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.
Agents which affect CELL DIVISION and the MITOTIC SPINDLE APPARATUS resulting in the loss or gain of whole CHROMOSOMES, thereby inducing an ANEUPLOIDY.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Deoxyribonucleic acid that makes up the genetic material of fungi.
Proteins found in any species of bacterium.
Very long DNA molecules and associated proteins, HISTONES, and non-histone chromosomal proteins (CHROMOSOMAL PROTEINS, NON-HISTONE). Normally 46 chromosomes, including two sex chromosomes are found in the nucleus of human cells. They carry the hereditary information of the individual.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).
A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
A bacterium causing tuberculosis in domestic fowl and other birds. In pigs, it may cause localized and sometimes disseminated disease. The organism occurs occasionally in sheep and cattle. It should be distinguished from the M. avium complex, which infects primarily humans.
Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.
DNA present in neoplastic tissue.
The rate dynamics in chemical or physical systems.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.
A specific protein in egg albumin that interacts with BIOTIN to render it unavailable to mammals, thereby producing biotin deficiency.
The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.
Acute infectious disease characterized by primary invasion of the urogenital tract. The etiologic agent, NEISSERIA GONORRHOEAE, was isolated by Neisser in 1879.
Europium. An element of the rare earth family of metals. It has the atomic symbol Eu, atomic number 63, and atomic weight 152. Europium is used in the form of its salts as coatings for cathode ray tubes and in the form of its organic derivatives as shift reagents in NMR spectroscopy.
A subdiscipline of genetics which deals with the cytological and molecular analysis of the CHROMOSOMES, and location of the GENES on chromosomes, and the movements of chromosomes during the CELL CYCLE.
One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence G/GATCC at the slash. BamHI is from Bacillus amyloliquefaciens N. Numerous isoschizomers have been identified. EC 3.1.21.-.
Identification of genetic carriers for a given trait.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Telomeric repeats on small polydisperse circular DNA (spcDNA) and genomic instability. (1/5773)

Small polydisperse circular DNA (spcDNA) is a heterogeneous population of extrachromosomal circular molecules present in a large variety of eukaryotic cells. Elevated amounts of total spcDNA are related to endogenous and induced genomic instability in rodent and human cells. We suggested spcDNA as a novel marker for genomic instability, and speculated that spcDNA might serve as a mutator. In this study, we examine the presence of telomeric sequences on spcDNA. We report for the first time the appearance of telomeric repeats in spcDNA molecules (tel-spcDNA) in rodent and human cells. Restriction enzyme analysis indicates that tel-spcDNA molecules harbor mostly, if not exclusively, telomeric repeats. In rodent cells, tel-spcDNA levels are higher in transformed than in normal cells and are enhanced by treatment with carcinogen. Tel-spcDNA is also detected in some human tumors and cell lines, but not in others. We suggest, that its levels in human cells may be primarily related to the amount of the chromosomal telomeric sequences. Tel-spcDNA may serve as a unique mutator, through specific mechanisms related to the telomeric repeats, which distinguish it from the total heterogeneous spcDNA population. It may affect telomere dynamics and genomic instability by clastogenic events, alterations of telomere size and sequestration of telomeric proteins.  (+info)

Polynucleotide probes that target a hypervariable region of 16S rRNA genes to identify bacterial isolates corresponding to bands of community fingerprints. (2/5773)

Temperature gradient gel electrophoresis (TGGE) is well suited for fingerprinting bacterial communities by separating PCR-amplified fragments of 16S rRNA genes (16S ribosomal DNA [rDNA]). A strategy was developed and was generally applicable for linking 16S rDNA from community fingerprints to pure culture isolates from the same habitat. For this, digoxigenin-labeled polynucleotide probes were generated by PCR, using bands excised from TGGE community fingerprints as a template, and applied in hybridizations with dot blotted 16S rDNA amplified from bacterial isolates. Within 16S rDNA, the hypervariable V6 region, corresponding to positions 984 to 1047 (Escherichia coli 16S rDNA sequence), which is a subset of the region used for TGGE (positions 968 to 1401), best met the criteria of high phylogenetic variability, required for sufficient probe specificity, and closely flanking conserved priming sites for amplification. Removal of flanking conserved bases was necessary to enable the differentiation of closely related species. This was achieved by 5' exonuclease digestion, terminated by phosphorothioate bonds which were synthesized into the primers. The remaining complementary strand was removed by single-strand-specific digestion. Standard hybridization with truncated probes allowed differentiation of bacteria which differed by only two bases within the probe target site and 1.2% within the complete 16S rDNA. However, a truncated probe, derived from an excised TGGE band of a rhizosphere community, hybridized with three phylogenetically related isolates with identical V6 sequences. Only one of the isolates comigrated with the excised band in TGGE, which was shown to be due to identical sequences, demonstrating the utility of a combined TGGE and V6 probe approach.  (+info)

The role of interleukin 12 in the development of atherosclerosis in ApoE-deficient mice. (3/5773)

The cytokine profile of atherosclerotic aortas from apoE-deficient mice was assessed by reverse transcriptase-polymerase chain reaction. The results clearly showed that the expression of mRNA for IL-12p40 was evident in aortas from 3-month-old apoE-deficient mice. The mRNA for IL-10 was detected in aorta from these mice at the age of 6 months, indicating that expression of IL-12 is earlier than that of IL-10 in these animals. Concurrent with IL-12p40, the mRNA for the T-cell cytokine IFN-gamma, but not IL-4, was detected in aortas of mice at young and old ages. Both in situ hybridization and immunostaining further demonstrated the localization of IL-12 in macrophages of atherosclerotic lesions. Immunohistochemistry also demonstrated the expression of costimulatory molecules B7-1 and B7-2 in macrophages, suggesting that activation of T lymphocytes by macrophages may occur via surface antigens in lesions. When the immunoglobulin isotype of the antioxidized LDL antibodies in sera of apoE-deficient mice was determined, it revealed that both IgM and IgG were present. Furthermore, IgG2a is predominant and comprises approximately 50% of the antioxidized LDL IgG in sera from young mice (3 months), but decreased to lower levels (35%) in older mice (6 months). Daily administration of IL-12 led to an increase in serum levels of antioxidized LDL antibodies and accelerated atherosclerosis in young apoE-deficient mice compared with control mice injected with PBS alone. Taken together, these data suggest that IL-12 plays an active role in regulating the immune response during the early phase of atherosclerosis in apoE-deficient mice.  (+info)

Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions. (4/5773)

Restriction fragment length polymorphisms (RFLPs) identified in the ribosomal-DNA (rDNA) repeat were used for molecular strain differentiation of the dermatophyte fungus Trichophyton rubrum. The polymorphisms were detected by hybridization of EcoRI-digested T. rubrum genomic DNAs with a probe amplified from the small-subunit (18S) rDNA and adjacent internal transcribed spacer (ITS) regions. The rDNA RFLPs mapped to the nontranscribed spacer (NTS) region of the rDNA repeat and appeared similar to those caused by short repetitive sequences in the intergenic spacers of other fungi. Fourteen individual RFLP patterns (DNA types A to N) were recognized among 50 random clinical isolates of T. rubrum. A majority of strains (19 of 50 [38%]) were characterized by one RFLP pattern (DNA type A), and four types (DNA types A to D) accounted for 78% (39 of 50) of all strains. The remaining types (DNA types E to N) were represented by one or two isolates only. A rapid and simple method was also developed for molecular species identification of dermatophyte fungi. The contiguous ITS and 5.8S rDNA regions were amplified from 17 common dermatophyte species by using the universal primers ITS 1 and ITS 4. Digestion of the amplified ITS products with the restriction endonuclease MvaI produced unique and easily identifiable fragment patterns for a majority of species. However, some closely related taxon pairs, such as T. rubrum-T. soudanense and T. quinkeanum-T. schoenlenii could not be distinguished. We conclude that RFLP analysis of the NTS and ITS intergenic regions of the rDNA repeat is a valuable technique both for molecular strain differentiation of T. rubrum and for species identification of common dermatophyte fungi.  (+info)

Identification of Mycobacterium kansasii by using a DNA probe (AccuProbe) and molecular techniques. (5/5773)

The newly formulated Mycobacterium kansasii AccuProbe was evaluated, and the results obtained with the new version were compared to the results obtained with the old version of this test by using 116 M. kansasii strains, 1 Mycobacterium gastri strain, and 19 strains of several mycobacterial species. The sensitivity of this new formulation was 97.4% and the specificity was 100%. Still, three M. kansasii strains were missed by this probe. To evaluate the variability within the species, genetic analyses of the hsp65 gene, the spacer sequence between the 16S and 23S rRNA genes, and the 16S rRNA gene of several M. kansasii AccuProbe-positive strains as well as all AccuProbe-negative strains were performed. Genetic analyses of the one M. gastri strain from the comparative assay and of two further M. gastri strains were included because of the identity of the 16S rRNA gene in M. gastri to that in M. kansasii. The data confirmed the genetic heterogeneity of M. kansasii. Furthermore, a subspecies with an unpublished hsp65 restriction pattern and spacer sequence was described. The genetic data indicate that all M. kansasii strains missed by the AccuProbe test belong to one subspecies, the newly described subspecies VI, as determined by the hsp65 restriction pattern and the spacer sequence. Since the M. kansasii strains that are missed are rare and all M. gastri strains are correctly negative, the new formulated AccuProbe provides a useful tool for the identification of M. kansasii.  (+info)

Development and characterization of complex DNA fingerprinting probes for the infectious yeast Candida dubliniensis. (6/5773)

Using a strategy to clone large genomic sequences containing repetitive elements from the infectious yeast Candida dubliniensis, the three unrelated sequences Cd1, Cd24, and Cd25, with respective molecular sizes of 15,500, 10,000, and 16,000 bp, were cloned and analyzed for their efficacy as DNA fingerprinting probes. Each generated a complex Southern blot hybridization pattern with endonuclease-digested genomic DNA. Cd1 generated an extremely variable pattern that contained all of the bands of the pattern generated by the repeat element RPS of Candida albicans. We demonstrated that Cd1 does not contain RPS but does contain a repeat element associated with RPS throughout the C. dubliniensis genome. The Cd1 pattern was the least stable over time both in vitro and in vivo and for that reason proved most effective in assessing microevolution. Cd24, which did not exhibit microevolution in vitro, was highly variable in vivo, suggesting in vivo-dependent microevolution. Cd25 was deemed the best probe for broad epidemiological studies, since it was the most stable over time, was the only truly C. dubliniensis-specific probe of the three, generated the most complex pattern, was distributed throughout all C. dubliniensis chromosomes, and separated a worldwide collection of 57 C. dubliniensis isolates into two distinct groups. The presence of a species-specific repetitive element in Cd25 adds weight to the already substantial evidence that C. dubliniensis represents a bona fide species.  (+info)

Molecular evidence for the existence of additional members of the order Chlamydiales. (7/5773)

Respiratory tract infections in man may be caused by several members of the genus Chlamydia and also by two Chlamydia-like strains, 'Simkania negevensis' (Z-agent) and 'Parachlamydia acanthamoebae' (Bng). To facilitate diagnostic procedures a PCR assay able to detect all known Chlamydiaceae sequences in one reaction was developed. For this purpose, primers were selected to amplify a fragment of the 16S rRNA gene. Characterization of the amplified fragments was done by hybridization with specific probes and by sequencing. PCR assays were carried out using DNA isolated from nose/throat specimens or from peripheral blood mononuclear cells of patients with respiratory tract infections, and from vessel wall specimens of abdominal aneurysms. Six of the 42 nose/throat swab specimens analysed yielded strong bands and one yielded a faint band. Three of these bands were identified as Chlamydia pneumoniae and one as Chlamydia trachomatis by sequencing. Analysis of the three other bands yielded two different new sequences. DNA isolated from peripheral blood mononuclear cells of one patient yielded a third new sequence. DNA isolated from peripheral blood mononuclear cells of four healthy controls was negative. One of the abdominal aneurysm specimens also yielded a strong band. Sequencing revealed a fourth new sequence. All negative controls included during specimen processing and PCR analysis remained negative. The typical secondary structure of microbial 16S genes was present in all four new sequences indicating the validity of the sequence data. All four new sequences were distinct from other bacteria and clustered together with known Chlamydiaceae sequences. Phylogenetic analysis suggested a new lineage, separating the four new sequences, 'S. negevensis' and 'P. acanthamoebae' from the genus Chlamydia with the four known chlamydial species. In conclusion, this study provides evidence for the existence of several new members of the order Chlamydiales. Since the source of the Chlamydia-like strains has not been identified and serological and/or molecular cross-reactivities may be expected, results of identification of infecting recognized organisms should be interpreted cautiously.  (+info)

Preimplantation diagnosis by fluorescence in situ hybridization using 13-, 16-, 18-, 21-, 22-, X-, and Y-chromosome probes. (8/5773)

PURPOSE: Our purpose was to select the proper chromosomes for preimplantation diagnosis based on aneuploidy distribution in abortuses and to carry out a feasibility study of preimplantation diagnosis for embryos using multiple-probe fluorescence in situ hybridization (FISH) on the selected chromosomes of biopsied blastomeres. METHODS: After determining the frequency distribution of aneuploidy found in abortuses, seven chromosomes were selected for FISH probes. Blastomeres were obtained from 33 abnormal or excess embryos. The chromosome complements of both the biopsied blastomeres and the remaining sibling blastomeres in each embryo were determined by FISH and compared to evaluate their preimplantation diagnostic potential. RESULTS: Chromosomes (16, 22, X, Y) and (13, 18, 21) were selected on the basis of the high aneuploid prevalence in abortuses for the former group and the presence of trisomy in the newborn for the latter. Thirty-six (72%) of 50 blastomeres gave signals to permit a diagnosis. Diagnoses made from biopsied blastomeres were consistent with the diagnoses made from the remaining sibling blastomeres in 18 embryos. In only 2 of 20 cases did the biopsied blastomere diagnosis and the embryo diagnosis not match. CONCLUSIONS: If FISH of biopsied blastomere was successful, a preimplantation diagnosis could be made with 10% error. When a combination of chromosome-13, -16, -18, -21, -22, -X, and -Y probes was used, up to 65% of the embryos destined to be aborted could be detected.  (+info)

PAC - Probe Array Controller. Looking for abbreviations of PAC? It is Probe Array Controller. Probe Array Controller listed as PAC
Cycling probe technology (CPT) is a molecular biological technique for detecting specific DNA sequences. CPT operates under isothermal conditions. In some applications, CPT offers an alternative to PCR. However, unlike PCR, CPT does not generate multiple copies of the target DNA itself, and the amplification of the signal is linear, in contrast to the geometric amplification of the target DNA in PCR. CPT uses a sequence specific chimeric probe which hybridizes to a complementary target DNA sequence and becomes a substrate for RNase H. Cleavage occurs at the RNA internucleotide linkages and results in dissociation of the probe from the target, thereby making it available for the next probe molecule. Integrated electrokinetic systems have been developed for use in CPT. Bhatt, R; Scott, B; Whitney, S; Bryan, R; et al. (1999). Detection of nucleic acids by cycling probe technology on magnetic particles: high sensitivity and ease of separation. Nucleosides Nucleotides. 18 (6): 1297-1299. Tang, T.; ...
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections ...
A synthetic oligonucleotide hybridization probe comprising at least one sequence at least 12 nucleotides long, the probe forms duplexes in which at least 80% of the nucleotides are base paired with any nucleotide sequence that is either substantially of the form (Xl...Xi)n in which X is any nucleotide; i which designates the number of nucleotides in the sequence is at least 2 and n is at least 2, or substantially of the form [(Xl...Xi)mY]w in which X is any nucleotide, Y is any group of 1 or more nucleotides and is different from (Xl...Xi), m is at least 1, w is 2, and i is at least 2, the number of nucleotides in said probe being less than 3i when i is greater than 3.
In this study, we found a PyA-modified adenine cluster (A-cluster), a minimum fluorescent unit for significant emission wavelength changes, and investigated its photophysical and structural properties. The basic A-cluster unit was an adenine-pentad duplex containing stacked PyA pairs in the center aligned in 2015 Hot Articles in Organic and Biomolecular Chemistry
Single nucleotide polymorphism (SNP) arrays are important tools widely used for genotyping and copy number estimation. This technology utilizes the specific affinity of fragmented DNA for binding to surface-attached oligonucleotide DNA probes. We analyze t...
A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a central window opening and a registration surface against which the substrate may be mounted. A gasket having a central opening defining an active area is mounted on the active surface of the substrate and has inlet and output extension openings which extend away from the central opening. A press block is engaged against the gasket to fully enclose an active volume between the press block, the peripheral walls of the central opening in the gasket, and the active surface of the substrate. A press is mounted to the base to selectively press the press block against the gasket and hold it in position. Channels in the press block extend to the extension openings in the gasket to allow flow of reagent into and out of the active volume, which is confined to the thickness of the gasket and can thus be minimized. The press may include a press screw which can be turned to engage against the
File probe level tells file opener (its probe method to be exact), how hard it should try to recognize the type of the file. File openers may ignore this or support only some probe levels, but if specifies reccomened behaviour. Before opening any file we detect its type by calling probe for every registered file opener (in order of priority) and passing GO_FILE_PROBE_FILE_NAME as probe level. If none of them recogizes the file, we increase probe level and try again.... ...
KP Technology is an award winning company designing and manufacturing Kelvin Probe systems for work function and surface potential measurements. We supply state of the art equipment and consultancy services to innovative companies and research institutes worldwide.
KP Technology is an award winning company designing and manufacturing Kelvin Probe systems for work function and surface potential measurements. We supply state of the art equipment and consultancy services to innovative companies and research institutes worldwide.
OligoWiz 2.0 is a powerful tool for microarray probe design that allows for integration of sequence annotation such as exon/intron structure, UTR regions, TSS, etc. In addition to probe selection according to a series of probe quality parameters, cross-hybridization, Δ Tm, position in transcript, probe folding and low-complexity, the program facilitates automatic placement of probes relative to the sequence annotation. The program also supports automatic placement of multiple probes per transcript. Together these facilities make advanced probe design feasible for scientists inexperienced in computerizes information management. Furthermore, we show that probes designed using OligoWiz 2.0 give rise to consistent hybridization results ...
FISH is a taxonomic staining method in which fluorescent DNA probes hybridise to ribosomal RNA in whole cells. FISH is culture independent and the test can be completed within 24 hours. T(D)GGE is a gel-electrophoretic separation procedure for double stranded deoxyribonucleic acids (dsDNA) produced by primer directed DNA amplification. T(D)GGE is a very useful method in order to monitor the effect of probiotics and antibiotics as well as other drugs on the intestinal microflora of human and animals. Also real time PCR can be used to monitor and quantify the presence of specific bacteria or even genes in the gut flora ...
DNA probes are the known short, single-stranded, labelled DNA sequences used to detect the presence or absence of nucleic acid in a sample.
WOODWARD, MJ and WRAY, C (1990) 9 DNA PROBES FOR DETECTION OF TOXIN AND ADHESIN GENES IN ESCHERICHIA-COLI ISOLATED FROM DIARRHEAL DISEASE IN ANIMALS ...
I am having a real hard time with this question I cant find it anywhere , please help . Here is the question : in the DNA fingerprinting the DNA probe that is used is ________________ to the DNA sequence of the repeats in the sample . Thank you .
Dive into the research topics of Mus spretus-specific line-1 dna probes applied to the cloning of the murine pearl locus. Together they form a unique fingerprint. ...
The TS2000-SE from MPI is the first ever 200mm automated engineering probe system on the market integrating innovative features specifically designed to reduce the cost of test. These features are incorporated into the MPI ShielDEnvironment™ for ultra-low noise, very accurate and highly reliable DC/CV, RF and High Power measurements.。Wafer Probe Stations | Probe Stations | 200mm Probe Stations | Automatic Probe Stations | 200mm Automated Probe Stations | Wafer Probe Systems | 200mm Probe System
E.I. du Pont de Nemours & Co. has bolstered its relationship with Molecular Biosystems by agreeing to acquire exclusive marketing rights for the San Diego-based companys DNA probe technology products in Japan, Taiwan and South Korea.. ...
A probe driving method and a probe apparatus for bringing a probe into contact with the surface of a sample in a safe and efficient manner by monitoring the probe height. Information about the height of the probe from the sample surface is obtained by detecting a probe shadow (54) appearing immediately before the probe contacts the sample, or based on a change in relative positions of a probe image and a sample image that are formed as an ion beam is irradiated diagonally.
Scientists have developed a fluorescent probe technology that can be switched on and off in the aqueous environment of cells to monitor biological processes.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon. ...
Probe Test Results: (Stated 90o pulse lengths are the best achieved during installation. To reduce risk of damage to the probes, facility users should use slightly lower RF nutation frequencies (i.e. longer 90o pulse lengths) where possible. Please discuss with the facility manager appropriate RF nutation frequencies for your experiments.). ...
This site provides an animated simulation to learn about the use of a probe to locate genes in specific organisms. A gene sequence is presented and step-by-step instructions are used to explain the uniqueness of DNA and how probes are designed. ...
Probe definition is - a slender medical instrument used especially for exploration (as of a wound or body cavity). How to use probe in a sentence. Synonym Discussion of probe.
How is Probe Alternation Link Self-Assembly Reaction abbreviated? PALSAR stands for Probe Alternation Link Self-Assembly Reaction. PALSAR is defined as Probe Alternation Link Self-Assembly Reaction very rarely.
or https:// means youve safely connected to the .gov website. Share sensitive information only on official, secure websites. ...
This OMP40-2 Probe includes a quick start guide, batteries and tool kit only. Ultra compact 3D touch-trigger probe with optical signal transmission. Used for workpiece set-up inspection on small and medium machining centres and the growing number of high speed machines fitted with small HSK and spindle tapers. Compatibility with all Renishaw optical receivers enables users to easily upgrade existing installations.
Tektronix Current Probes provide high sensitivity for accurate power and load analysis. Find the right Current Probe for your measurement needs.
Choose Tektronix Passive Probes to accurately visualize signals over a broad range of applications. Find the right Passive Probe for your needs.
DNA-based digital tension probes(a) Theoretical plot showing the expected increase in fluorescence signal asa function of applied force for the 100% and 22% GC-
In order to accurately predict the DNAm ages, the final matrix must contain information about all features (probes). Sometimes, due to scanning or hybridization problems, the information about some probes will be unavailable, leading to errors. In case your data have missing probes, try to use inputations methods to fill this gap. If there are no missing probes, just upload your dataset ...
Gentaur molecular products has all kinds of products like :search , Biolin \ SensiMix II Probe \ BIO-91002 for more molecular products just contact us
Browse a comprehensive selection of Test Leads and Probes from a wide variety of Electrical Products available on ColeParmer.com. Shop now!
Buy PRO Scientific Saw-Tooth Multi-Prep Probes, 10 mm; 12/Pk and more from our comprehensive selection of PRO Scientific Homogenizer Accessories
The Huntleigh 8 MHz Wide Beam Vascular Probe has been designed to produce a distinctly wide beam which completely encompasses the vessel of interest.
Which type you have depends on the size of the cancer, where it is, how much it has spread, how well the rest of your liver is working, and other factors.
EMEA Oligonucleotide DNA Microarrays (oDNA) market analysis of an industry is a crucial thing for various stakeholders like investors, CEOs, traders, suppliers and others. The Oligonucleotide DNA Microarrays (oDNA) industry research report is a resource, which provides current as well as upcoming technical and financial details of the industry.. Oligonucleotide DNA Microarrays (oDNA) market 2017-2022 research report is a professional and in-depth study on the current state of this market. Various definitions and classification of the industry, applications of the industry and chain structure are given. Present day status of the Oligonucleotide DNA Microarrays (oDNA) industry policies and news are analysed.. Following are major Table of Content of Oligonucleotide DNA Microarrays (oDNA) Industry:. Oligonucleotide DNA Microarrays (oDNA) Market Competition by Manufacturers, Oligonucleotide DNA Microarrays (oDNA) Production, Revenue by Region, Oligonucleotide DNA Microarrays (oDNA) Supply, ...
Human chromosome 20 has not been sufficiently mapped, as only four DNA probes detecting RFLPs and 18 genes have been assigned to this chromosome. Using a chromosome 20-specific library to isolate and characterize new low-copy DNA probes, we found a new polymorphic DNA probe (pS43), which is assigned to human chromosome 20q13.. Note: Institut fur Humangenetik der Universitat, Gottingen, Federal Republic of Germany.. ...
TY - JOUR. T1 - Characteristics of a DNA probe (pa3′HVR) when used for paternity testing. AU - Allen, R. W.. PY - 1989/1/1. Y1 - 1989/1/1. N2 - DNA probes that detect polymorphic loci in the human genome are finding widespread application in many areas of genetic testing. Paternity testing represents one area for the application of probe technology; this report presents data obtained in a paternity testing program with a probe (pa3′HVR) derived from a locus (D16S85) approximately 8 kilobases (kb) downstream from the alpha globin gene complex on chromosome 16. The pa3′HVR probe used under stringent conditions of hybridization detects a highly polymorphic locus in chromosomal DNA digested with Pvu 2 restriction endonuclease. Alleles at the D16S85 locus were grouped into 58 size bins differing from one another by 100 base pairs in the black and white populations. The most common alleles detected in whites fell into the 2.3‐kb group with a collective frequency of 0.1849. In blacks, the most ...
Imininingwane 48 Rxns, 96 Rxns No. Inani Lezinto Ingxenye eyinhloko 48 Rxns 96 Rxns 1 I-ejenti yokukhipha i-Nucleic acid 1.4mL / tube 2
A ball grid technology circuit assembly for mounting and connecting a ball grid array circuit package having a grid array of ball pads to a printed circuit board and providing enhanced connections for testing of the ball grid array circuits. The printed circuit board includes a via array of vias wherein each via extends from the upper face of the printed circuit board to a lower face of the printed circuit board and is co-located with a ball pad of the ball grid array to provide a path between the ball pad and the lower end of the via to provide electrical access to the corresponding ball pad. A probe array includes a pin array of probe pins wherein each probe pin is co-located with a via of the via array to provide electrical contact with a ball pad of the ball grid array. The probe array is mounted in a probe block and the probe block and printed circuit board include alignment holes and alignment pins for aligning the probe array with the vias and a probe attachment to secure the probe block to the
Synthesis of Single-stranded DNA Probes of Heterogeneous Length from Bacteriophage M13 Templates Technique yields a heterogeneous population of short radiolabeled molecules 200-300 nucleotides in length. These probes are synthesized, as in Synthesis of Single-stranded DNA Probes of Defined Length from Bacteriophage M13 Templates, by extension of an oligonucleotide primer on a single-stranded DNA template. The radiolabeled products of the reaction are then separated from the template by electrophoresis through a denaturing gel from which they are eluted directly into hybridization buffer. ...
Read independent reviews on Southern-Light™ and Southern-Star™ Chemiluminescence Nucleic Acid Detection System from Thermo Fisher Scientific on SelectScience
Beijing Wantai Biological item WS-1248 Beijing Wantai Biological WS-1248 Nucleic Acid Detection Kit COVID. Contact Sanbio for more information.
Surface Enhanced Raman as Tool to study Drug Protein Interactions and Nucleic Acid Detection. Chandrabhas Narayana (BHAS) Chemistry and Physics of Materials Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bangalore 560064, India [email protected] Slideshow 2716597 by raven
Probes are mapped using two different programs according to probe length - short probes (Bowtie2 alignment, while longer probes (,200bp) are mapped using Exonerate. The reason for processing the probes differently according to length is based on the need to rapidly map large numers of probes across many different species. The majority of VectorBase probes are short (Bowtie2 alignments which are significantly faster to perform than Exonerate. Longer probes (,200bp) are often derived from cDNA/EST sequences and span multiple exon/intron junctions - these probes are mapped with Exonerate as it detects these junctions in an accurate and sensitive manner. Probes are mapped against both the genomic sequence, and the transcripts. Where high quality hits are found on the transcript but not on the genomic sequence the probe sequence is projected from the transcript sequence back into genomic coordinates.. Alignments are stored as described by the Ensembl microarray probe mapping pipeline, but only probes ...
Southern blotting is an experimental procedure where DNA, from a genomic or other source, is digested with a restriction enzyme and then separated by size using gel electrophoresis. The fragments are transferred from the gel onto a membrane (blotted) which is then incubated with a labelled single-stranded DNA probe. Such a procedure allows one to locate a particular sequence of DNA within a complex mixture of DNA. From a gene targeting perspective, Southern blotting can be used to detect whether a targeting event has successfully taken place. Designing a good Southern blot probe for a particular gene or locus involves finding a stretch of DNA sequence at that locus, generally 500-1000bp long, that has the desirable qualities of being unique to that locus, with little or no repetitive DNA content. Molecular biologists tend to design their probes manually, by excising portions of genome sequence from online genome browsers (such as such as Ensembl) and then pasting them into a genome-search ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
MetaSystems XT ALK BA DNA Probe XT ALK BA Break Apart Probe Life Sciences:Molecular Biology Reagents and Kits:PCR and qPCR:PCR Reagents and Kits:qPCR Master Mixes:Probe qPCR
... Introduction ...Affymetrix has designed new algorithms for monitoring GeneChip &re...- Calculation of statistical significance for detection and chan...This technical note reviews the design and testing for Affymetrix ...,New,statistical,algorithms,for,Monitoring,Gene,Expression,on,GeneChip®,Probe,Arrays,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
To exploit fully the potential of current sequencing technologies for population-based studies, one must enrich for loci from the human genome. Here we evaluate the hybridization-based approach by using oligonucleotide capture probes in solution to enrich for approximately 3.9 Mb of sequence target. We demonstrate that the tiling probe frequency is important for generating sequence data with high uniform coverage of targets. We obtained 93% sensitivity to detect SNPs, with a calling accuracy greater than 99%.
The TS3000-SE is the consequent further development of the TS3000 probe system equipped with MPI ShielDEnvironment™ for ultra-low noise, extremely accurate and highly reliable DC/CV, 1/f, RTS and RF measurements, addressing primarily the needs of the Device Characterization, Wafer Level Reliability and RF & mmW applications.The exclusive, actively cooled probe platen design provides maximal stability over the wide temperature range from -60° to 300°C and is making the TS3000-SE probe system an excellent choice for testing devices under different thermal conditions.300mm Automatic Probe Station | Probe System | Automated Probe Station | Probe Systems | 300mm Probe Stations | 300mm Wafer Probe System
Copyright © 2020 NCL of Wisconsin, Inc. - North Central Laboratories - Water and Wastewater Lab Supplies. All rights reserved ...
Definition of Nucleic acid hybridization with photos and pictures, translations, sample usage, and additional links for more information.
The present invention is directed to providing sensitive and accurate assays for genotyping with a minimum or absence of target-specific amplification.
Methods and kits for use in detecting a target nucleic acid in a sample are disclosed. In one particular application, the methods and kits allow for the detection of an undesirable micro-organism (e.g. Listeria, Salmonella or Enterobacteriaceae) in food or present on a food preparation surface.
A white-box approach to microarray probe response characterization: the BaFL pipeline. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
MetaSystems Probes is proud to offer a wide range of high quality DNA probes that reach a new standard in reliability of the results.
MetaSystems Probes is proud to offer a wide range of high quality DNA probes that reach a new standard in reliability of the results.
The Voluson S8 supports a wide range of advanced 2D and 3D probes, which enable high quality images with exceptional reliability. System intelligence and probe technology combine to produce outstanding image quality with minimal user interaction - just place the probe and scan with confidence.
Our Specialized Laser Unit: ProHealth Physiotherapy is using medical grade laser built by Theralase (www.theralase.com). The advanced laser controller unit can be connected to 3 different probes, depending on the therapeutic effect desired: a single probe, a triple probe, and a multiple probe. The single (point) probe has only one invisible laser emitter, and is used for very local applications or needle-free acupuncture. The triple probe has one invisible laser emitter (905 nm) and two visible red emitters (660nm). The multiple probe has 5 invisible laser emitters and 4 visible red laser emitters. The combination between the super pulsed invisible laser light (905 nm) and the continuous wave visible red laser light (660nm) allow simultaneous superficial and deep (up to 4) laser treatment, thus activating all three known cellular pathways ...
Figure S1. (A) Pilot microarray. miRNA probes were printed to test hybridization and wash conditions. Samples for hybridization to the array were individually labeled with either (Cy3, green) or (Cy5, red), mixed in equamolar amounts, and hybridized to the array at the temperatures indicated. Specific hybridization was observed as either red or green spots on the array, whereas crosshybridization was observed as shades of yellow. Each miRNA probe was selected to test the resolution of the array for single and multiple mismatches between probe sequences. For example, fug-mir-197 and hsa-mir-197 test the resolution of single nucleotide differences within three nucleotides of the 3_ end of the probe sequence. Test probes were also designed to span a range of melting temperatures. As the hybridization temperature was increased, the specificity of the test array was generally improved to a point near 55-60 C (see hsa-mir-148 and hsa-mir-136). (B) Representative sample grid of a microarray. A more ...
Custom FISH Probes - Creative Bioarray is pleased to announce the introduction of custom labeled Fluorescence in situ Hybridization (FISH) Probes for a range of molecular and cytogenetic applications. What sets us apart in custom labeled probe manufacturing is our ability to consistently offer high quality locus-specific probes that can be customized to meet the specific needs of researchers and clinicians alike.
As police begin the identification of victims from the New Zealand volcano tragedy, cruise passengers claim they were never warned about any potential dangers.
Figure 4.6. Inlet 5-hp during a measurement (left), various multi-hole probes (right).​ A large set of pneumatic probes is available in the laboratory, and new probes can be built to suit the requirement for the measurement. Additive manufacturing is used to produce probes with complex shapes in met
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe. Info: FAM; Same primer pair and probe as used in qPCR assay qRnoCIP0037245; intron_spanning ...
ddPCR™ probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe. Info: FAM; Same primer pair and probe as used in qPCR assay qRnoCIP0037388; intron_spanning ...
Probe size is important because longer probes hybridize less specifically than shorter probes, so that short strands of DNA or ... Repetitive DNA sequences must be blocked by adding short fragments of DNA to the sample. The probe is then applied to the ... Preparing DNA probes for one species and performing FISH with this probe allows one to visualize the distribution of this ... The mixture of probe sequences determines the type of feature the probe can detect. Probes that hybridize along an entire ...
Sigma Life Science provides products such as custom DNA/RNA oligos; custom DNA and LNA probes; siRNA; isotopically-labelled ...
Langdale, Jane Alison (1985). Gene detection using immobilized DNA probes. london.ac.uk (PhD thesis). University of London. ... "DNA probes in human disease". Biochemical Society Symposium. 53: 131-43. PMID 3332764. ... Langdale, J. A.; Malcolm, A. D. B. (1985). "A rapid method of gene detection using DNA bound to Sephacryl". Gene. 36 (3): 201- ...
"Probing Allostery Through DNA". Science. 339 (6121): 816-819. Bibcode:2013Sci...339..816K. doi:10.1126/science.1229223. PMC ... Elf, Johan; Li, Gene-Wei; Xie, X. Sunney (2007). "Probing Transcription Factor Dynamics at the Single-Molecule Level in a ... Yu, Ji; Xiao, Jie; Ren, Xiaojia; Lao, Kaiqin; Xie, X. Sunney (2006). "Probing Gene Expression in Live Cells, One Protein ... Xie, X. Sunney; Dunn, Robert C. (1994). "Probing Single Molecule Dynamics". Science. 265 (5170): 361-364. Bibcode:1994Sci...265 ...
2012) "Labeling of DNA Probes by Nick Translation". Molecular Cloning: A Laboratory Manual. Park, K; Kim, J; Lim, S; Han, S; ... There are many different types of FISH probes available, such as repeat probes, probes that detect specific genes or telomeres ... CISH probes are approximately 20 nucleotides in length and are designed for DNA targets. They are complementary to the targeted ... Probe design for CISH is very similar to that for FISH with differences only in labelling and detection. FISH probes are ...
The multicolour probes attach to a certain DNA fragment. MLPA is a test that finds and records DNA copy change numbers through ... Lastly, EHMT1 sequencing is a process in which a single-strand of DNA from the EHMT1 gene is removed, and DNA polymerase is ... The geneticists examined how a missense mutation would affect the function of the DNA by looking at DNA models. After, they ... tracks chromosome deletions and or amplifications using fluorescent dyes on genomic sequences of DNA samples. The DNA samples ( ...
"Array-based electrical detection of DNA with nanoparticle probes". Science. 295 (5559): 1503-1506. doi:10.1126/science.1067003 ... When strands of DNA bins to the oligonucleotides it closes a gap between two electrodes, changing the conductivity. She worked ... Her research was the first to show that DNA could be used to form nanoparticle assemblies with tuneable inter-particle ... Her doctoral research on the physical properties of DNA-linked nanoparticles was awarded the American Chemical Society Nobel ...
"DNA profile created in body probe". BBC News. 2012-03-14. Retrieved 2018-10-09. "Pathologist test to start on exhumed Sutton ... In 2013 police stated that they had added the woman's DNA profile to the national database in the hope that a match would be ... In 2012, the body was exhumed so that a DNA profile could be created. The profile was compared to samples from five families, ... "DNA breakthrough in Sutton Bank body case". Gazette & Herald. Retrieved 2018-10-06. Laville, Sandra (2013-01-04). "'Sutton Bank ...
Kilvington, Simon; Beeching, John (June 1995). "Identification and epidemiological typing of Naegleria fowleri with DNA probes ...
"Melanie Hall murder probe: DNA evidence discovered". BBC News. 8 June 2016. Retrieved 2017-07-26. "KRIMINALITÄT: "Etwas ganz, ... "New DNA evidence leads to arrest in 1980 slaying of 14-year-old babysitter". Los Angeles Times. 1 December 2017. Retrieved 18 ... "DNA positively identifies 'Grateful Doe' as man missing from Myrtle Beach". WMBF News. NBC. 9 December 2015. Retrieved 9 ... "DNA confirms remains found in 2015 belong to Atsumi Yoshikubo, missing Japanese tourist". CBC. 14 April 2016. Retrieved 21 ...
Commercial DNA probe hybridization tests (e.g., AccuProbe Streptococcus pneumoniae culture identification test; Gen-Probe, San ...
Chin made the same algorithem-based obvious argument in DNA probes.[86] ... a b A. Chin.Artful prior art and the quality of DNA patents. Ala. L. Rev. 57 (2005): 975. "Archived copy" (PDF). Archived (PDF) ... Gene Probes are Unpatentable Printed Matter. Fed. Cir. BJ, 20, p.527. "Archived copy" (PDF). Archived (PDF) from the original ... has demonstrated a method to protect DNA research.,[83] which could apply to other technology. Chin wrote an algorithm to ...
"Deletion mapping of human chromosome 5 using chromosome-specific DNA probes". Am. J. Hum. Genet. 37 (5): 839-52. PMC 1684692. ...
Zhang J, Smaga LP, Satyavolu NS, Chan J, Lu Y (December 2017). "DNA Aptamer-Based Activatable Probes for Photoacoustic Imaging ... An mRNA molecule transfers a portion of the DNA code to other parts of the cell for making proteins. DNA therapeutics needs ... Messenger RNA (mRNA) is a single-stranded RNA molecule that is complementary to one of the DNA strands of a gene. ... Broadly, aptamers are small molecules composed of either single-stranded DNA or RNA and are typically 20-100 nucleotides in ...
Chin made the same algorithm-based obvious argument in DNA probes. Google and other technology companies founded the LOT ... Gene Probes are Unpatentable Printed Matter. Fed. Cir. BJ, 20, p.527. "Archived copy" (PDF). Archived (PDF) from the original ... For example, a Professor of Law at the University of North Carolina School of Law, has demonstrated a method to protect DNA ... A. Chin.Artful prior art and the quality of DNA patents. Ala. L. Rev. 57 (2005): 975. "Archived copy" (PDF). Archived (PDF) ...
"DNA tests probe the genomic ancestry of Brazilians". Brazilian Journal of Medical and Biological Research. 42 (10): 870-876. ... Lopes, Reinaldo José (October 5, 2009). "DNA de brasileiro é 80% europeu, indica estudo" [Brazilian DNA is 80% European, study ... inherited Y-DNA from a male African ancestor, and less than 10% inherited Y-DNA from a male Amerindian ancestor. As these tests ... According to a DNA study from 2010, "a new portrayal of each ethnicity contribution to the DNA of Brazilians, obtained with ...
"DNA tests probe the genomic ancestry of Brazilians". Brazilian Journal of Medical and Biological Research. 42 (10): 870-876. ... According to an autosomal DNA study from 2010, "a new portrayal of each ethnicity contribution to the DNA of Brazilians, ... Similar DNA tests showed that people self-classified as Mulatto or White and Black mix, span from 62% to 85% European (mostly ... DNA de brasileiro é 80% europeu, indica estudo. .folha.uol.com.br (1970-01-01). Retrieved on 2012-05-19. De Assis Poiares, ...
DNA tests probe the genomic ancestry of Brazilians. Introduction, first paragraph.: Little is known about the number of ... Pena, S. D. J.; Bastos-Rodrigues, L.; Pimenta, J. R.; Bydlowski, S. P. (October 1, 2009). "DNA tests probe the genomic ancestry ... According to an autosomal DNA study from 2010, "a new portrayal of each ethnicity contribution to the DNA of Brazilians, ... DNA de brasileiro é 80% europeu, indica estudo. .folha.uol.com.br (1 January 1970). Retrieved on 2012-05-19. De Assis Poiares, ...
The immobilized RNA is then probed using DNA. (1996) Eastern-western blot was first used by Bogdanov et al. The method involved ... This could be seen as similar to a Southern blot, however the interaction is between a DNA molecule (the aptamer) and a protein ... The membrane is then probed with antibodies for epitopes of interest. This method has also been discussed in later work by the ... in that they use lectin probes and other staining reagents. (2009) Eastern blot has been used to describe a blot of proteins on ...
Pena, S.D.J.; Bastos-Rodrigues, L.; Pimenta, J.R.; Bydlowski, S.P. (11 September 2009). "DNA tests probe the genomic ancestry ... The mitochondrial DNA (mtDNA) is present in all human beings and passed down through the maternal line, i.e. the mother of a ... DNA de negros famosos retrata Brasil mestiço Revista Veja. José Edward. v. 33, n. 51, 12-20-2000. "Quem Somos Nós?" Xuxa ... The mitochondrial DNA and Y chromosome suffer only minor mutations through centuries, thus can be used to establish the ...
Pena, S.D.J.; Bastos-Rodrigues, L.; Pimenta, J.R.; Bydlowski, S.P. (11 September 2009). "DNA tests probe the genomic ancestry ... According to a 2013 autosomal DNA study, the ancestral composition of the state of Minas Gerais can be described as: 59.20% ... Lopes, Reinaldo José (5 October 2009). "DNA de brasileiro é 80% europeu, indica estudo". Folha de S.Paulo. "Brasil 500 anos". ... "). "A new portrayal of each ethnicity contribution to the DNA of Brazilians, obtained with samples from the five regions of ...
Pena, S.D.J.; Bastos-Rodrigues, L.; Pimenta, J.R.; Bydlowski, S.P. (2009). "DNA tests probe the genomic ancestry of Brazilians ... According to a DNA study from 2010, a new portrayal of each ethnicity contribution to the DNA of Brazilians, obtained with ... "DNA de brasileiro é 80% europeu, indica estudo". uol.com.br. Lins TC, Vieira RG, Abreu BS, Grattapaglia D, Pereira RW (2010). " ... A 2015 autosomal DNA study found Chile to be 55.16% European, 42.38% Native American and 2.44% African, using LAMP-LD modeling ...
Deith J (15 October 2020). "Scientists probe possible DNA link to 'Long Covid'". Channel 4 News. Retrieved 18 October 2020. ...
ISBN 0-7506-3083-3. Pena, S.D.J.; Bastos-Rodrigues, L.; Pimenta, J.R.; Bydlowski, S.P. (11 September 2009). "DNA tests probe ...
"DNA tests probe the genomic ancestry of Brazilians". Brazilian Journal of Medical and Biological Research. 42 (10): 870-76. doi ... This is consistent with the estimate based on Y-chromosomal DNA, which places the split between ca. 806-447 kya. In terms of ... April 2007). "Mitochondrial DNA variation of modern Tuscans supports the near eastern origin of Etruscans". American Journal of ... 2009). "Human mitochondrial DNA variation in Southern Italy". Annals of Human Biology. 36 (6): 785-811. doi:10.3109/ ...
They can be differentiated from M. tuberculosis and each other via commercially available DNA probes.[3] :245 ... proposed the name M. chimaera for strains that a reverse hybridization-based line probe assay suggested belonged to MAIS (M. ...
DNA tests probe the genomic ancestry of Brazilians". Brazilian Journal of Medical and Biological Research. 42 (10): 870-876. ... "Folha Online - Ciência - DNA de brasileiro é 80% europeu, indica estudo - 05/10/2009". Retrieved 14 May 2015.. ... According to an autosomal DNA study (the autosomal study is about the sum of the ancestors of a person, unlike mtDNA or yDNA ... Only autosomal DNA testing can reveal admixture structures, since it analyses millions of alleles from both maternal and ...
... high-force probing of DNA-protein interactions with magnetic tweezers". Methods. Single molecule probing by fluorescence and ... By turning the magnets, torsional stress can be applied to the DNA molecule. Rotations in the sense of the DNA helix are ... such as DNA Holliday junctions, DNA hairpins, or entire nucleosomes and chromatin. By acting upon the magnetic bead with the ... A double-stranded DNA molecule is fixed with multiple binding sites on one end to a glass surface and on the other to a ...
"Jomon Genes Using DNA, researchers probe the genetic origins of modern Japanese".. ... "Making sense of DNA data and the origins of the Japanese". Heritage of Japan. 2009-02-27. Retrieved 2016-12-05.. ... One of the main goals of the study was to create an archive of DNA samples which could be used to identify which diseases are ... The Japanese DNA sequence consists of 24.2% Korean, 16.1% Okinawa, 4.8% Uniquely Japanese, 25.8% Chinese, 8.1% Ainu, and 21% ...
It is used as a molecular probe to detect DNA or RNA. It can easily be attached to nucleotides by chemical modifications. DIG ... Once hybridisation occurs in situ, RNA probes with the incorporated DIG-U can be detected with anti-DIG antibodies conjugated ... molecules are often linked to uridine nucleotides; DIG-labeled uridine (DIG-U) can then be incorporated into RNA probes via in ...
Molecular and Cellular Probes. 31: 22-27. doi:10.1016/j.mcp.2016.08.003. PMID 27523487. Archived (PDF) from the original on 20 ... The examination of preserved museum specimens has found Borrelia DNA in an infected Ixodes ricinus tick from Germany that dates ... Except for one study in Europe,[229] much of the data implicating lizards is based on DNA detection of the spirochete and has ... Polymerase chain reaction (PCR) tests for Lyme disease have also been developed to detect the genetic material (DNA) of the ...
These proteins tightly bind and condense the DNA, and are in part responsible for resistance to UV light and DNA-damaging ... are used as probes to verify that an autoclaved item has been rendered truly sterile: a small capsule containing the spores is ... Finally, DNA repair enzymes contained within the endospore are able to repair damaged DNA during germination. ... The DNA is replicated and a membrane wall known as a spore septum begins to form between it and the rest of the cell. The ...
8,0 8,1 8,2 Deu, E. et al., (2012). New approaches for dissecting protease functions to improve probe development and drug ... Ghatak, S. et al., (2015). A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis. JBT, nr 24(4), ... mida kasutatakse DNA ja RNA eraldamiseks bioloogilisest materjalist[12][13] ja papaiin, mida kasutatakse rakkude isoleerimiseks ...
To overcome sever line-broadening in native DNA, sheer-degraded natural DNA was prepared and studied to learn about the ... NMR is also useful for probing the binding of nucleic acid molecules to other molecules, such as proteins or drugs. This can be ... For duplex DNA nonexchangeable protons the H6/H8 protons on the base correlate to their counterparts on neighboring bases and ... The first NMR spectrum of a double-helical DNA was published in 1977[9] using a synthetic, 30-base-pair double helix. ...
OMOTENASHI, designed by JAXA, is a lander probe to study the lunar radiation environment.[31][38] ... "BioSentinel: DNA Damage-and-Repair Experiment Beyond Low Earth Orbit" (PDF). NASA Ames Research Center. Archived from the ...
2002). DNA-Modified nanocrystalline diamond thin-films as stable, biologically active substrates. Nature Materials, 1(4), 253-7 ... electronic wire probes, or other types of detectors. An LB film can then be transferred to a solid substrate by dipping the ...
මෙහිදී RNA, DNA (මෙම විෂයේ දී complementary DNA හෝ cDNA නමින් හැඳින්වේ) බවට reverse transcription කිරීම සහ අවශ්‍ය DNA ඉලක්ක ... Multiplex ligation-dependent probe amplification. History. & people. *Kary Mullis. *Kjell Kleppe (no) ...
Repair of psoralen DNA adducts[edit]. PUVA treatment produces both DNA interstrand crosslinks (ICLs) and monoadducts. The ICLs ... Cimino GD, Gamper HB, Isaacs ST, Hearst JE (1985). "Psoralens as Photoactive Probes of Nucleic Acid Structure and Function: ... defects in DNA repair increase gene copy number variation". DNA Repair (Amst.). 21: 87-96. doi:10.1016/j.dnarep.2014.05.011. ... the affinity of the psoralen for DNA is directly related to the concentration of the psoralen in the DNA chamber after ...
Hybridization of the probe to a specific DNA fragment on the filter membrane indicates that this fragment contains DNA sequence ... rather than DNA. To ensure the specificity of the binding of the probe to the sample DNA, most common hybridization methods use ... A probe that hybridizes only to a single DNA segment that has not been cut by the restriction enzyme will produce a single band ... The probe DNA is labelled so that it can be detected, usually by incorporating radioactivity or tagging the molecule with a ...
Fluorescein is currently used most as a fluorescent probe. Equipment that can automatically measure and calculate the capacity ... and protection of DNA, proteins and lipids from oxidative damage pursuant to Article 13(1) of Regulation (EC) No 1924/20061". ... "Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe ...
... and how to collect DNA evidence at a crime scene (forensics). ... to a series of remarkable discoveries that succeeded in probing ...
As described in the applications section, spectrophotometry can be used in both qualitative and quantitative analysis of DNA, ... Pump-probe spectroscopy. *Saturated spectroscopy. *Scanning tunneling spectroscopy. *Spectrophotometry. *Time-resolved ... Spectrophotometry is an important technique used in many biochemical experiments that involve DNA, RNA, and protein isolation, ...
A non-viral PDGF "bio patch" can regenerate missing or damaged bone by delivering DNA in a nano-sized particle directly into ... "Detection of c-sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization". In Vitro Cell ...
"Cyclin-dependent kinases are inactivated by a combination of p21 and Thr-14/Tyr-15 phosphorylation after UV-induced DNA damage ... "Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family". Proc. Natl. Acad. Sci. ...
P. M.F.J. Costa, X. Fang, S. Wang, Y. He, Y. Bando, M. Mitome, J. Zou, H. Huang and D. Golberg : «Two-probe electrical ... C. Zhi, Y. Bando, W. Wang, C. Tang, H. Kuwahara and D. Golberg : «DNA-Mediated Assembly of Boron Nitride Nanotubes» Chem. Asian ... L. Gilburd, X. G. Xu, Y. Bando, D. Golberg, G.C. Walker : «Near-field infared pump-probe imaging of surface phonon coupling in ... D. Golberg, M. Mitome, K. Kurashima, C. Y. Zhi, C. C. Tang, Y. Bando and O. Lourie : «In situ electrical probing and bias- ...
... about half the width of a molecule of DNA) in length to one approximately 10.6 light years (about 62 trillion miles) long. ... "Nine-Year Wilkinson Microwave Anisotropy Probe (WMAP) Observations: Final Maps and Results". The Astrophysical Journal ... "Five-Year Wilkinson Microwave Anisotropy Probe (WMAP) Observations: Data Processing, Sky Maps, and Basic Results" (PDF) ...
These results, found by probing with immunoassays, have been challenged by studies that interrogate DNA sequence by bisulfite ... This DNA-histone structure is called a nucleosome; the more tightly the DNA is bound by the nucleosome, and the more tightly a ... In mammals, methylation occurs on DNA and histone proteins. DNA methylation occurs on the cytosine of CpG dinucleotides in the ... DNA methylation. In Alzheimer's disease cases, global DNA hypomethylation and gene-specific hypermethylation has been observed ...
New Probe Detects Trace Pollutants in Groundwater". Oak Ridge National Laboratory Review. 26 (2).. ... SERS can be used to target specific DNA and RNA sequences using a combination of gold and silver nanoparticles and Raman-active ... The gold nanoparticles facilitate the formation of a silver coating on the dye-labeled regions of DNA or RNA, allowing SERS to ... Cao, Y. C.; Jin, R; Mirkin, CA (2002). "Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection". Science ...
quantitative PCR does not require this, as the detection system uses fluorescence and probes to detect the DNA molecules as ... DNA sequencing, a method developed by Walter Gilbert and Frederick Sanger in 1977,[5] caused a rapid change the development of ... Most DNA viruses assemble in the nucleus while most RNA viruses develop solely in cytoplasm.[22][23] ... Once the virus has gained access to the host's cells, the virus' genetic material (RNA or DNA) must be introduced to the cell. ...
Homologs of the recA gene, including rad51, play a key role in homologous recombinational repair of DNA during meiosis. A rad51 ... "CDC Probes Salmonella Outbreak, Health Officials Say Bacteria May Have Spread Through Some Form Of Produce". CBS News. 30 ... In a scientific review of potential claims for lycopene favorably affecting DNA, skin exposed to ultraviolet radiation, heart ... "Scientific Opinion on the substantiation of health claims related to lycopene and protection of DNA, proteins and lipids from ...
DNA and Cell Biology. 9 (3): 149-57. doi:10.1089/dna.1990.9.149. PMID 2111143.. ... Tarle I, Borhani DW, Wilson DK, Quiocho FA, Petrash JM (December 1993). "Probing the active site of human aldose reductase. ...
... even DNA from anyone deemed 'relevant' to an investigation."[89] Wyden offered an amendment to reform the "business-records ... "FBI Director Comey fired amid Russia probe". ABC News.. *^ "Wyden Statement on Firing of FBI Director James Comey". wyden. ...
... a nonstructural protein that degrades RBP1 and turns off the host cell's ability to transcribe DNA.[51] NS2 interferes with the ... "Probing the attenuation and protective efficacy of a candidate chikungunya virus vaccine in mice with compromised interferon ( ...
For instance, one can start with the gene for a protein that binds a certain sequence of DNA, and, by directing an unnatural ... allowing biochemists or structural biologists to probe or change the protein's function. ... amino acid with a reactive side-chain into the binding site, create a new protein that cuts the DNA at the target-sequence, ...
For example, synthetic peptides can be used as probes to see where protein-peptide interactions occur- see the page on Protein ... DNA, RNA, etc.), or to complex macromolecular assemblies.[3] Finally, while aspects of the lab techniques applied to peptides ...
The DNA match between the skeleton and Giuliano's relations means that Sicilian prosecutors are now archiving the probe they ... On October 15, 2010, the Public Prosecutor's Office in Palermo decided to exhume the body and compare its DNA with living ... The DNA tests showed a 90% likelihood that the skeleton belongs to Giuliano. ...
They discovered that in the presence of two TWIST protein molecules combined together, the TWIST gene functions as a DNA ... Common methods include PCR, multiplex ligation-dependent probe amplification (MLPA), and chromosomal microarray (CMA). ... The majority of the identified mutations in the TWIST gene interfere with how the protein attaches to DNA, preventing the ... Prenatal testing can also be performed during weeks 10-12 using chorionic villus sampling (CVS) to extract DNA from the fetus. ...
Molecular spectroscopy deals with the response (spectrum) of molecules interacting with probing signals of known energy (or ... including biopolymers such as DNA. Molecules commonly used as building blocks for organic synthesis have a dimension of a few ... "Mapping the electrostatic force field of single molecules from high-resolution scanning probe images". Nature Communications ...
INTERNATIONAL ATOMIC ENERGY AGENCY, Radioactively Labelled DNA Probes for Crop Improvement, IAEA-TECDOC-1253, IAEA, Vienna ( ...
... the probe forms duplexes in which at least 80% of the nucleotides are base paired with any nucleotide sequence that is either ... the number of nucleotides in said probe being less than 3i when i is greater than 3. ... A synthetic oligonucleotide hybridization probe comprising at least one sequence at least 12 nucleotides long, ... In contrast to the results obtained with the two probes discussed above, the second probe 0-33.15, a 32 base long probe ...
DNA stable-isotope probing.. Neufeld JD1, Vohra J, Dumont MG, Lueders T, Manefield M, Friedrich MW, Murrell JC. ... The separation of DNA and retrieval of unlabeled and labeled fractions can be performed in 4-5 days, with much of the time ... Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of ... The protocol includes a modification of existing published methods, which maximizes the recovery of labeled DNA from CsCl ...
Probing the DNA of Death. Experts examine 14,994 remains to try to identify the 9/11 dead. In the process, they are reinventing ... The DNA in these cellular power plants is inherited directly from each persons mother. It is much smaller than DNA found in ... Since June, DNA experts at Celera Genomics in Rockville, Md., have processed 19,000 DNA specimens from victims and relatives, ... In recent weeks, the forensic experts have invented more refined testing techniques to extract usable DNA. They are retesting ...
Transcription factor proteins, nucleosomes and other DNA-binding molecules may bend DNA and bring distant segments of DNA into ... Novel Technique Probes Critical DNA-Protein Interactions. in NEWS By applying a repulsive electric field at the gold surface to ... Novel Technique Probes Critical DNA-Protein Interactions. Posted 9 years ago on Monday, January 18th, 2010. in NEWS ... Novel Technique Probes Critical DNA-Protein Interactions *Shining Light on the Problem: New Laser Technique Boosts Accuracy of ...
1986)‎. The use of DNA probes for malaria diagnosis. [‎Geneva]‎ : World Health Organization. http://www.who.int/iris/handle/ ...
... and functional aspects of DNA and RNA research. ... V. V. Didenko, "Dna probes using fluorescence resonance energy ... Multipyrene Tandem Probes for Point Mutations Detection in DNA. Svetlana A. Kholodar, Darya S. Novopashina, Mariya I. ... P. L. Paris, J. M. Langenhan, and E. T. Kool, "Probing DNA sequences in solution with a monomer-excimer fluorescence color ... T. Bryld, T. Hojland, and J. Wengel, "DNA-selective hybridization and dual strand invasion of short double-stranded DNA using ...
A DNA test is being conducted to determine if a newborn boy found dead on a Bushwick street last month is related to a dead ... A DNA test is being conducted to determine if a newborn boy found dead on a Bushwick street last month is related to a dead ... Theyve decided that even if DNA tests reveal the two didnt come from the same mother, the tots are - in a way befitting life ... John Corbisiero, head of the precincts detective squad, said the medical examiner is performing DNA tests to determine if ...
DNA Probe Testing Gen-Probe™ PACE Transport Received Incorrect Test Requested or Written Request Default Action (Change) Gen- ...
Research at Western Carolina University on DNA sequencing used in crime labs has gotten a $718,000 grant from the National ... Research at Western Carolina University on DNA sequencing used in crime labs has gotten a $718,000 grant from the National ... Mark Wilson, director of the Forensic Science Program at WCU, has been evaluating new DNA sequencing instrumentation for use in ... which is led by Wilson and top scientists specializing in the area of forensic DNA analysis. ...
Molecular cloning of a species-specific DNA probe for Campylobacter jejuni. Mol. Cell. Probes 1:245-259.PubMedCrossRefGoogle ... DNA probes assays for detection of Campylobacter and Salmonella. In Nucleic Acid and Monoclonal Antibody Probes, ed. B. ... Development of a Salmonella-specific biotinylated DNA probe for rapid routine identification of Salmonella. Mol. Cell. Probes 2 ... Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes. J. Clin. ...
Broad range DNA probes for detecting and amplifying eubacterial nucleic acids.. Chen K1, Neimark H, Rumore P, Steinman CR. ... Such broadly homologous probes offer advantages over more narrowly specific probes for detecting organisms whose identity is ... coli DNA was detected in the presence of a large excess of eukaryotic DNA. Information useful for partial phylogenetic ... One or both of the probes can detect all of 23 phylogenetically diverse eubacterial nucleic acids against which they were ...
Neisseria gonorrhoeae DNA Probe,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative ...
Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of ... DNA stable-isotope probing Nat Protoc. 2007;2(4):860-6. doi: 10.1038/nprot.2007.109. ... The separation of DNA and retrieval of unlabeled and labeled fractions can be performed in 4-5 days, with much of the time ... Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of ...
... and the DNA was gel purified again to eliminate the low-molecular-weight DNA and adapter dimers. We suspended 1 μl of the DNA ... Probe-Mixture Constitution. The following nine repeated DNA sequences were used for karyotyping: coumarin-5-dUTP-labeled knob ... Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Akio Kato, ... Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize ...
DNA probes that store mechanical information reveal transient piconewton forces applied by T cells. Rong Ma, Anna V. Kellner, ... DNA-based digital tension probes reveal integrin forces during early cell adhesion. Nat. Commun. 5, 5167 (2014).. ... A DNA-based molecular probe for optically reporting cellular traction forces. Nat. Methods 11, 1229-1232 (2014).. ... For example, DNA-based tension probes have revealed roles for mechanics in platelet, B cell, T cell, and fibroblast function. ...
DNA potentially may have played an earlier role in the case. It was just coming into use as a criminal investigative tool in ... Investigators lacked DNA evidence so Snellings death and the burglaries werent included in the tally of Golden State Killer ... But others say it raises troubling legal and privacy concerns for the millions of people who submit their DNA to such sites to ... "People who submit DNA for ancestors testing are unwittingly becoming genetic informants on their innocent family," Mercer said ...
A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a ... A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:. (a) a base having ... 1. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:. (a) a base ... 8. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:. (a) a base ...
DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT imaging S. S. Agasti, Y. Wang, F. Schueder, A. Sukumar, R. ... Herein, we report a universal approach for the creation of DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT ... Exchange-PAINT, a recently developed DNA-based multiplexing approach, in theory facilitates spectrally-unlimited multiplexing ... its widespread application has been hampered by the availability of DNA-conjugated ligands for protein labeling. ...
... Moodie, Lindon W. K. Umeå University, Faculty of Science and ... Furthermore, results from DNA cross-linking and imaging studies of clickable analogues enforce the hypothesis that colibactin ... click chemistry, colibactin, DNA damage, microbiome, photochemistry National Category Biochemistry and Molecular Biology ... which is believed to alkylate DNA. To investigate the influence of the putative "warhead" and the prodrug strategy on ...
... Moodie, Lindon W. K. Umeå universitet, Teknisk-naturvetenskapliga ... Furthermore, results from DNA cross-linking and imaging studies of clickable analogues enforce the hypothesis that colibactin ... click chemistry, colibactin, DNA damage, microbiome, photochemistry Nationell ämneskategori Biokemi och molekylärbiologi ... which is believed to alkylate DNA. To investigate the influence of the putative "warhead" and the prodrug strategy on ...
... By growing polymers on a porous aluminum oxide template, researchers at ... This work is detailed in a paper titled, "Dual-functionalized polymer nanotubes as substrates for molecular-probe and DNA- ... phys.org/news/2006-05-polymer-nanotubes-molecular-probes-dna.html This document is subject to copyright. Apart from any fair ... The piece of DNA that the researchers chose recognizes and binds to the BRCA1 tumor suppressor gene, mutations in which ...
In addition, the team used HiDRA to examine how disease risk DNA variants in regulatory elements affect gene expression ... Approximately 98 percent of the human genome is made up of noncoding DNA, including enhancers, promoters, and other elements ... By building on these approaches, HiDRA lets researchers create massive libraries of regulatory DNA and study their influence ... the team applied HiDRA to a blood cell line to test about seven million noncoding DNA fragments for ones that regulate gene ...
Synonyms for DNA probe at Thesaurus.com with free online thesaurus, antonyms, and definitions. Find descriptive alternatives ... More words related to DNA probe. DNA fingerprinting. noun. . method of identification of individuals by comparing dna ...
... GUATEMALA CITY (Reuters) -- Prosecutors investigating the slaying last year ... The DNA tests -- believed to be the first to be performed on members of Guatemalas powerful military -- will be sent on ... Prosecutor Celvin Galindo requested that DNA tests be performed on 12 military officers and five other people, including Mario ... of a Roman Catholic bishop began running DNA tests on 12 military officers and a priest on Wednesday, hoping science can solve ...
... magnetic nanomaterial probes (ZMPs) which are very suitable for the construction of such magnetic probes [16, 17]. DNA, as DNA- ... When 1 mg/mL DNA was added to ZMPs-HRP-AFP Ab2 to form the nanochain probes (DNA/(ZMPs-HRP-AFP Ab2)n), its TEM(Figure 2(c)) ... DNA can specifically combine with ZrO2 [10], but if DNA was directly used to fix ZrO2 to make probes, it will be very difficult ... After the binding of antigen, the electrode was incubated in 50 μL of 5 mg/mL DNA/(HRP-AFP Ab2)n magnetic probe. Finally the ...
... the potential of DNA probes-based diagnostics is huge. DNA probes also render themselves as a powerful tool for chromosomal ... GIA announces the release of a comprehensive global report on DNA Probe-Based Diagnostics markets. The global market for DNA ... as stated by the new market research report on DNA Probes-Based Diagnostics. In the US, the rapid growth of DNA-based ... Also, the boom in the molecular diagnostics market is beginning to echo downstream into the DNA-probe based diagnostics segment ...
In a bind after a latest forensic report showed the DNA of the child delivered by a minor rape victim did not match with that ... DNA Of 10-Year-Olds Baby Doesnt Match With Rapist. Cops Reboot Probe. The report from the Central Forensic Sciences ... has pointed out that the DNA sample of the delivered child and the alleged rapist, maternal uncle, did not match.. The DNA ... Chandigarh: In a bind after a latest forensic report showed the DNA of the child delivered by a minor rape victim did not match ...
We have measured the bending elasticity of short double-stranded DNA (dsDNA) chains through small-angle X-ray scattering from ... We have measured the bending elasticity of short double-stranded DNA (dsDNA) chains through small-angle X-ray scattering from ... We have measured the bending elasticity of short double-stranded DNA (dsDNA) chains through small-angle X-ray scattering from ... Mastroianni, Alexander; Sivak, David; Geissler, Phillip & Alivisatos, Paul. Probing the Conformational Distributions of Sub- ...
Home › News › 2010 › December › International team including UNC scientist probes DNA function * News ... It turns out that particularly important stretches of DNA in the genome are "conserved," or retained throughout evolutionary ... Lieb studies chromatin, the protein superstructure that packages DNA and controls which sections of the genome are accessible ... was authored by Lieb and other members of the model organism ENCyclopedia Of DNA Elements (modENCODE) Consortium, which is ...
  • DNA stable-isotope probing. (nih.gov)
  • Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate particular substrates are identified without the prerequisite of cultivation. (nih.gov)
  • Marine sediment slurries enriched for anaerobic, sulfate-reducing prokaryotic communities utilizing glucose and acetate were used to provide the first comparison between stable-isotope probing (SIP) of phospholipid fatty acids (PLFA) and DNA (16S rRNA and dsrA genes) biomarkers. (wiley.com)
  • DNA stable isotope probing (DNA-SIP) allows for the identification of microbes that assimilate isotopically labeled substrates into DNA. (springer.com)
  • DeRito CM, Pumphrey GM, Madsen EL (2005) Use of field-based stable isotope probing to identify adapted populations and track carbon flow through a phenol-degrading soil microbial community. (springer.com)
  • Buckley DH, Huangyutitham V, Hsu S-F, Nelson TA (2007) Stable isotope probing with 15 N 2 reveals novel noncultivated diazotrophs in soil. (springer.com)
  • Neufeld JD, Schäfer H, Cox MJ et al (2007) Stable-isotope probing implicates Methylophaga spp and novel Gammaproteobacteria in marine methanol and methylamine metabolism. (springer.com)
  • Kovatcheva-Datchary P, Egert M, Maathuis A et al (2009) Linking phylogenetic identities of bacteria to starch fermentation in an in vitro model of the large intestine by RNA-based stable isotope probing. (springer.com)
  • Egert M, De Graaf AA, Maathuis A et al (2007) Identification of glucose-fermenting bacteria present in an in vitro model of the human intestine by RNA-stable isotope probing. (springer.com)
  • Buckley DH, Huangyutitham V, Hsu S-F, Nelson TA (2008) 15 N 2 -DNA-stable isotope probing of diazotrophic methanotrophs in soil. (springer.com)
  • Radajewski S, Ineson P, Parekh NR, Murrell JC (2000) Stable-isotope probing as a tool in microbial ecology. (springer.com)
  • Kasai Y, Takahata Y, Manefield M, Watanabe K (2006) RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater. (springer.com)
  • Youngblut ND, Buckley DH (2014) Intra-genomic variation in G + C content and its implications for DNA stable isotope probing. (springer.com)
  • Hungate BA, Mau RL, Schwartz E et al (2015) Quantitative microbial ecology through stable isotope probing. (springer.com)
  • Youngblut ND, Barnett SE, Buckley DH (2018) HTSSIP: An R package for analysis of high throughput sequencing data from nucleic acid stable isotope probing (SIP) experiments. (springer.com)
  • To measure these changes in real time, the researchers anchored fluorescent-labeled DNA molecules to electrodes on the gold surface of the TUM platform, and applied an electric field to orient the fluorophores in a well-ordered manner conducive to effective detection of conformational changes. (bu.edu)
  • When you introduce a protein in solution, the protein will bind to the DNA and change its shape, resulting in a change in the spectral signature we observe from the fluorescent molecules attached to the DNA," said Ünlü. (bu.edu)
  • The researchers next plan to combine electrical orientation of fluorescent-tagged DNA with a new optical technique developed by Unlu that uses self-interference fluorescence microscopy (SSFM) to more precisely measure the height of the fluorophores. (bu.edu)
  • 5′-Pyrene modified oligonucleotide provides a highly sensitive fluorescent probe of RNA," Nucleic Acids Research , vol. 27, no. 11, pp. 2387-2392, 1999. (hindawi.com)
  • These probes, coupled with changes in the chromosome-preparation procedure that improve fluorescent signal detection, allowed the development of a FISH karyotyping method that is effective on all tested maize lines. (pnas.org)
  • In addition, the investigators conducted a set of imaging experiments in which they used a short stretch of DNA as a targeting molecule and a fluorescent dye as the imaging agent. (phys.org)
  • Here, dynamic DNA complexes detect a specific DNA-conjugated antibody and undergo strand displacement to liberate a quencher strand and activate a fluorescent reporter. (rice.edu)
  • A group of scientists at Nagoya University, Japan, have developed an incredibly versatile DNA fluorescent dye, named 'Kakshine' after a former NU student of its members, Dr. Kakishi Uno, but it also means to make the nucleus shine brightly, since the nucleus is pronounced 'Kaku' in Japanese. (medica-tradefair.com)
  • Kakshine is an exceptionally versatile new dyeing agent which improves upon the capabilities of and solves the shortcomings of current-generation fluorescent dyes in DNA imaging. (medica-tradefair.com)
  • In the present study, we describe a novel fluorescent probe construct, termed molecular zipper, which is specially designed for quantifying target DNA by real-time monitoring RAM reactions. (scientistsolutions.com)
  • Zelenin, A. 2004-10-16 00:00:00 To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S-28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barleyHordeum vulgareL. (deepdyve.com)
  • V. A. Efimov, A. A. Buryakova, S. V. Reverdatto, and O. G. Chekhmakhcheva, "Use of N-methylimidazolide phosphotriester method for the synthesis of oligonucleotides useful in recombinant DNA studies," Russian Journal of Bioorganic Chemistry , vol. 9, no. 10, pp. 1376-1381, 1983. (hindawi.com)
  • Herein, we present a mechanoselection strategy that uses locking oligonucleotides to preferentially and irreversibly bind DNA probes that are mechanically strained over probes at rest. (pnas.org)
  • Upon addition of unlocking oligonucleotides that drive toehold-mediated strand displacement, the probes reset to the real-time state, thereby erasing stored mechanical information. (pnas.org)
  • DNA aptamers are synthetic, single-stranded DNA oligonucleotides selectedby SELEX methods for their binding with specific ligands. (mdpi.com)
  • For manufacturing the unimolecular double-stranded DNA microarrays, two kinds of special single-stranded oligonucleotides, constant oligonucleotide and target oligonucleotide, were chemically synthesized. (mdpi.com)
  • The constant oligonucleotides with internal aminated dT were used to capture and immobilize the target oligonucleotides onto the solid surface, and also to provide a primer for later enzymatic extension reactions, while target oligonucleotides took the role of harbouring DNA-binding sites of DNA-binding proteins. (mdpi.com)
  • These cyanine probes show high binding affinity to oligonucleotides but different binding preferences to various secondary structures. (spie.org)
  • A comparison between the thiol-derivatised immobilisation approach and a reference immobilisation method, based on the coupling of biotinylated oligonucleotide probes onto a streptavidin coated dextran sensor surface, using synthetic complementary oligonucleotides has been discussed in detail. (science24.com)
  • Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy," Nucleic Acids Research , vol. 34, no. 10, pp. 3161-3168, 2006. (hindawi.com)
  • Detection of nucleic acids in situ: novel oligonucleotide analogues for target-assembled DNA-mounted exciplexes," Organic and Biomolecular Chemistry , vol. 5, no. 7, pp. 1039-1051, 2007. (hindawi.com)
  • 1989. Detection of rotavirus by hybridization with a nonradioactive synthetic DNA probe and comparison with commercial enzyme immunoassays and silver stained Polyacrylamide gels. (springer.com)
  • Detection of enterotoxigenic Escherichia coli by dot blot hybridization with biotinylated DNA probes. (springer.com)
  • With a sandwich immunoassay, one DNA-derived magnetic nanoprobe, simplified as DNA/(ZMPs-HRP-AFP Ab 2 ) n , was employed for the detection of AFP. (hindawi.com)
  • Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit , enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. (neb.com)
  • In areas where histoplasmosis is not endemic, including Taiwan, serologic tests, antigen detection reagents, and specific DNA probes for diagnosis of histoplasmosis are not universally available. (thefreedictionary.com)
  • The spectroscopic measurements in the presence of well matched and mismatched DNA support the notion that our systems are interesting probes for mismatch detection and signaling. (caltech.edu)
  • A DNA probe for the specific detection and identification of Streptococcus oralis was isolated from HindII-digested DNA of S. oralis NCTC 11427 and cloned in Escherichia coli. (asm.org)
  • Finally, the probe complexes were reconfigured to act as AND-gates for the detection of co-localized proteins. (rice.edu)
  • A commercial DNA probe kit (Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires' disease in 167 patients with pneumonia. (nih.gov)
  • Here, the volume-amplified magnetic nanobead detection assay (VAM-NDA) is for the first time applied for detection of rolling circle amplified (RCA) DNA molecules in a portable, commercial AC susceptometer that operates at ambient temperatures and with an analysis time of about 20 min. (diva-portal.org)
  • The findings show that the VAM-NDA holds promise for future wide-spread implementation in commercial AC susceptometer setups thus opening up for the possibility to perform magnetic bead-based DNA detection in point-of-care and outpatient settings. (diva-portal.org)
  • This thesis describes a new approach to biomolecular analysis, called the volume-amplified magnetic nanobead detection assay (VAM-DNA). (diva-portal.org)
  • Detection of plasmid-mediated beta-lactamases with DNA probes. (asm.org)
  • Rolling-circle amplification (RCA) and ramification amplification (RAM, also known as hyperbranched RCA) are isothermal nucleic acid amplification technologies that have gained a great application in in situ signal amplification, DNA and protein microarray assays, single nucleotide polymorphism detection, as well as clinical diagnosis. (scientistsolutions.com)
  • Real-time detection of RCA or RAM products has been a challenge because of most real-time detection systems, including Taqman and Molecular Beacon, are designed for thermal cycling-based DNA amplification technology. (scientistsolutions.com)
  • While biomedical researchers have long traced the onset of many diseases to genetic code irregularities, they're paying increased attention to another potential source: a class of molecules that bind to specific sequences of the double stranded DNA (dsDNA) molecules that contain the human body's operating instructions-and alter their shape in the process. (bu.edu)
  • Transcription factor proteins, nucleosomes and other DNA-binding molecules may bend DNA and bring distant segments of DNA into close proximity, thereby causing genes to turn on or off. (bu.edu)
  • By better understanding the impact of DNA-binding molecules upon different DNA sequences, scientists hope to accelerate efforts to discover the causes of and potential treatments for a wide range of genetic diseases. (bu.edu)
  • Since this intensity increases with height above the gold surface, the researchers could deduce any resulting height changes in segments of the DNA molecules-and thus pinpoint corresponding changes in their shape. (bu.edu)
  • In the National Science Foundation study, Spuhler introduced a protein called E. Coli Integration Host Factor (IHF) that can bend DNA molecules by as much as 180 degrees, and observed protein-induced bending upon the binding of IHF to the dsDNA probes. (bu.edu)
  • They aim to eventually use SSFM to analyze different protein-DNA interactions and to identify and characterize specific proteins that induce considerable shape changes in DNA molecules. (bu.edu)
  • Exchange-PAINT, a recently developed DNA-based multiplexing approach, in theory facilitates spectrally-unlimited multiplexing by sequentially imaging target molecules using orthogonal dye-labeled 'imager' strands. (rsc.org)
  • Nanozirconium dioxide (nano-ZrO 2 ), as a kind of Lewis acid, can be directly used to fix the antibody and enzyme through combining the carboxylic acid [ 12 , 13 ] of protein molecules with the phosphoric acid groups [ 14 ] (such as Lewis strong alkali) of DNA. (hindawi.com)
  • Lieb studies chromatin, the protein superstructure that packages DNA and controls which sections of the genome are accessible to regulatory molecules that convert the genetic code into cellular action. (unc.edu)
  • All cells carry chromosomes - large molecules of double-stranded DNA that are capped off by single-strand sequences called telomeres. (innovations-report.com)
  • In this thesis, the structural dynamics of water molecules and counterions in the hydration shell of DNA is investigated by two-dimensional infrared (2D IR) spectroscopy and pump-probe transient spectroscopy. (hu-berlin.de)
  • QDFM is based on hybridization of non-isotopically labeled probes onto DNA molecules that were bound to a solid support and stretched homogeneously to ~2.3 kb/µm. (caltech.edu)
  • Dna are the coding molecules found in all living things on earth which contain the instructions on how to build living things. (healthtap.com)
  • The obvious induced circular dichroism signals in circular dichroism spectra reveal that the molecules can specifically interact with DNA. (spie.org)
  • Others mechanically disturb or modify short molecules and therefore do not obtain flexibility properties of unperturbed and pristine DNA. (soton.ac.uk)
  • Double-stranded DNA must disentangle itself into single strands during replication or repair to allow functional molecules to bind and perform their various operations. (nanotech-now.com)
  • The protein molecules alone are smaller in molecular size than the protein-DNA complexes, leading to a less effective steric stabilization of the nanoparticles. (nanotech-now.com)
  • Sequence-specific conjugates of oligo(2′-O-methylribonucleotides) and hairpin oligocarboxamide minor-groove binders: design, synthesis, and binding studies with double-stranded DNA," Chemistry & Biodiversity , vol. 2, no. 7, pp. 936-952, 2005. (hindawi.com)
  • We have measured the bending elasticity of short double-stranded DNA (dsDNA) chains through small-angle X-ray scattering from solutions of dsDNA-linked dimers of gold nanoparticles. (unt.edu)
  • The invention relates to nucleic acid multiplexes, and more particularly to methods wherein quadruplexes are formed by specific binding between double-stranded nucleic acid probes and double-stranded nucleic acid target sequences. (google.com)
  • We present a novel method for fabricating unimole cular double-stranded DNA microarrays on solid surfaces, which were used to probe sequence-specific DNA/protein interactions. (mdpi.com)
  • Double-stranded DNA with SNPs cannot bind together so closely. (nanotech-now.com)
  • 2. A synthetic DNA hybridization probe as defined by claim which comprises a mixture of sequences each at least 12 nucleotides long. (google.com.au)
  • 6. A probe as defined by claim 4 which is more than 23 nucleotides in length and which forms at least 80% matched duplexes with any sequences as defined in subparagraphs (a) and (b) of claim 1 when the hybridization is accomplished at 65 and the post-hybridization washes are accomplished at temperatures sufficiently high to leave only duplexes where at least 80% of the nucleotides are base paired. (google.com.au)
  • Ultimately, scientists could use the platform to systematically investigate the interactions between selected proteins and hundreds of thousands of different DNA sequences in a single experiment. (bu.edu)
  • In this report we describe and characterize two oligomer probes that are broadly homologous to conserved eubacterial 16S ribosomal RNA (rRNA) sequences not present in human 18 rRNA or human mitochondrial 12S rRNA. (nih.gov)
  • By using these oligomer sequences or their complements as primers in the polymerase chain reaction (PCR), the equivalent of 1 pg of E. coli DNA was detected in the presence of a large excess of eukaryotic DNA. (nih.gov)
  • Information useful for partial phylogenetic classification of detected organisms may be obtained by direct sequence analysis of the amplified DNA and comparison with known sequences or catalogs. (nih.gov)
  • The procedure uses tandemly repeated DNA sequences to generate a distinctive banding pattern for each of the 10 chromosomes. (pnas.org)
  • Screening was carried out on random PCR libraries to recover sequences that were used as FISH probes to identify the somatic chromosomes. (pnas.org)
  • DNA probes can also be used to identify the presence of complementary sequences in a mix of fragments. (thefreedictionary.com)
  • a DNA copy of mRNA which contains only regulatory and coding sequences, i.e. introns have been removed. (thefreedictionary.com)
  • These were more diagnostic, with branched odd-chain fatty acids ( i 15:0, a 15:0 and 15:1ω6) possibly indicating the presence of Desulfococcus or Desulfosarcina sulfate-reducing bacteria (SRB) and sequences related to these SRB were in the 13 C-acetate-DNA dsrA gene library. (wiley.com)
  • The 13 C-acetate-DNA 16S rRNA gene library also contained sequences closely related to SRB, but these were the acetate-utilizing Desulfobacter sp. (wiley.com)
  • As demonstrated in representative hybridizations, vector-specific probes provide valuable information about molecule integrity, insert size and orientation as well as localization of hybridization domains relative to specifically-marked vector sequences. (caltech.edu)
  • Simulation-guided probe systems designed against 44 sequences of different target single-nucleotide variants showed between a 200- and 3,000-fold (median 890) higher binding affinity than their corresponding wild-type sequences. (rice.edu)
  • The phylogenetic relatedness of 15 stocks of Trypanosoma evansi, three stocks of Trypanosoma equiperdum and one stock of Trypanosoma brucei brucei was determined using Southern blot analysis of restriction enzyme digested DNA, probed with two repetitive DNA sequences from T b. brucei. (eurekamag.com)
  • However, most classical functional microarrays use specific probes that monitor only known sequences, and so fail to cover the full microbial gene diversity present in complex environments. (sciweavers.org)
  • The Random Primer DNA Labeling Kit, Version 2 is designed for radioactive labeling for hybridization probes. (clontech.com)
  • This probe labeling method overcomes many of the disadvantages of conventional nick translation procedures while producing hybridization probes from very small amounts of DNA (10 to 20 ng). (clontech.com)
  • The Radioactive Labeling for Hybridization Probes kit can also be used to label DNA fragments that are embedded in low-melting temperature agarose gel slices. (clontech.com)
  • Locus specific cDNA hybridization probes, particularly probes for genes of Class II loci, for use in the typing procedure are described. (patents.com)
  • Patent No. 6,828,097, "Single Copy Genomic Hybridization Probes and Method of Generating the Same," was granted in December 2004, and patent No. 7,014,997, "Chromosomal Structural Abnormality Localization with Single Copy Probes" was granted in March 2006. (technologynetworks.com)
  • An advanced technology for qPCR and SNP genotyping, the BHQplus® probe is a compact oligo fortified with our signature duplex stabilizing chemistry to elevate the melting temperature and enhance target specificity. (biosearchtech.com)
  • Quantifying DNA-protein interaction using DNA microarrays are gaining increasing attention due to their ability to profile specificity of interactions in a high-throughput manner. (mit.edu)
  • Previous studies have shown that rhodium metalloinsertors bind mismatches in double-strained DNA with high specificity. (caltech.edu)
  • Here we show that simulations of the binding kinetics are both necessary and sufficient to design nucleic acid probe systems with consistently high specificity as they enable the discovery of an optimal combination of thermodynamic parameters. (rice.edu)
  • The accessibility and specificity of the sequence-specific DNA-binding proteins binding to the immobilized unimolecular dsDNA probes were demonstrated by the binding of Cy3 labeled NF-?B (p50·p50) to the unimolecular dsDNA microarray. (mdpi.com)
  • Single copy DNA probes offer a specificity in hybridizing genetic chromosomes not heretofore available for identifying elusive strains of inherited genetic diseases and cancers, allowing, among other things, for more precise clinical treatment. (technologynetworks.com)
  • This work is detailed in a paper titled, "Dual-functionalized polymer nanotubes as substrates for molecular-probe and DNA-carrier applications. (phys.org)
  • Because most companies specialize in producing either substrates or DNA probes , many companies have formed partnerships in order to compete in the DNA chip market. (thefreedictionary.com)
  • By merging two powerful sequencing-based assays with a machine learning-based tool, a research team led by Xinchen Wang and associate members Melina Claussnitzer and Manolis Kellis in the Broad's Metabolism and Epigenomics programs, respectively, have engineered a powerful new approach for measuring individual noncoding DNA segments' ability to control gene expression, and doing so at both massive scale and high resolution. (broadinstitute.org)
  • On my medical records, it says that a test that was taken was performed using the bd probetec chlamydia trachomatis and neisseria gonorrhoeoae amplified DNA assays, does that indicate that it was a DNA probe test? (healthtap.com)
  • I'm currently have some trouble screening a ZAP II library composed of genomic DNA of my bacteria of interest. (bio.net)
  • Because the probes are not specific to a particular disease, the technology provides a genomic-based bacterial identification system that does not require a one-to-one ratio of DNA probes to pathogenic species. (phys.org)
  • More recent genomic identification methods that use polymerase chain reaction, or PCR, and genomic sequencing can be faster but require expensive equipment and training as well as a specific DNA probe for each pathogen to be tested. (phys.org)
  • From a genomic DNA library of Lactobacillus delbrueckii subsp. (asm.org)
  • As a demonstration of the usefulness of this simulation-guided design approach, we developed probes that, in combination with PCR amplification, detect low concentrations of variant alleles (1%) in human genomic DNA. (rice.edu)
  • Genomic DNA probes were made for five members of the Anopheles punctulatus complex of mosquitoes found in Papua New Guinea. (ajtmh.org)
  • farauti No. 6, by differentially screening total genomic DNA libraries of individual species and sibling species with homologous DNA against heterologous DNA labeled with 32 P. Probes ranged from 273 to 630 bp. (ajtmh.org)
  • We were able to retrieve, from published melting temperatures, several established flexibility properties such as the presence of highly flexible TATA regions of genomic DNA and support recent findings that DNA is very flexible at short length scales. (soton.ac.uk)
  • We plan to further develop this assay into a hassle-free genotyping assay to detect SNPs in real biological samples containing long genomic DNA," says Tan. (nanotech-now.com)
  • Probes may be synthesized in the laboratory, with a sequence complementary to the target DNA sequence. (thefreedictionary.com)
  • A DNA sequence labelled with a radioactive element used to identify the position of a segment with the complementary sequence by binding to it. (thefreedictionary.com)
  • The probes are snippets of complementary DNA from a known disease. (phys.org)
  • If the DNA from a patient's sample binds with the complementary DNA in a disease probe, the diagnosis is positive for that disease. (phys.org)
  • Rather than identifying a target strain based on a 100 percent match with a specific probe, Rice's system tests how well the target DNA binds with several different random segments of complementary DNA. (phys.org)
  • Each nanocircle consists of DNA base pairs arranged in a sequence that is complementary to the telomere. (innovations-report.com)
  • Numbered 1002877 in the Patent Gazette, the patented method named Probe Alternation Link Self-Assembly Reaction (PALSAR), uses two DNA probes that cover three complementary areas to amplify genetic signals by repeating hybridization. (thefreedictionary.com)
  • A single-strand DNA fragment used to detect the complementary fragment. (tabers.com)
  • The VAM-NDA consists of three basic steps: biomolecular target recognition, enzymatic amplification of the probe-target complex using the rolling circle amplification (RCA) technique, and addition of target complementary probe-tagged magnetic nanobeads which exhibit Brownian relaxation behavior. (diva-portal.org)
  • 1. A DNA probe that is specific to a single Class II HLA locus comprising a labeled DNA sequence that is substantially complementary to the DNA sequence at said locus. (patents.com)
  • View the selection of fluorophore-quencher combinations for dual-labeled probes available from LGC Biosearch. (biosearchtech.com)
  • We synthesize high quality dual-labeled probes followed by HPLC purification to ensure every order meets purity requirements. (biosearchtech.com)
  • Dual-Labeled Probes are the most common probe type for qPCR and are often referred to as hydrolysis probes. (sigmaaldrich.com)
  • BHQnova™ probes have improved quenching efficiency compared to traditional end-labeled probes, while also improving signal release upon amplification. (biosearchtech.com)
  • gonorrhoeae DNA amplification test, but, neg. (healthtap.com)
  • Amplification probes are nucleic acid based and very specific for either gonorhrea or chlamydia, and not for trichimonasis. (healthtap.com)
  • The Company plans to combine the probes with its proprietary signal amplification technologies, which will enhance their effectiveness by allowing visualization at levels not achievable by other methods. (technologynetworks.com)
  • One or both of the probes can detect all of 23 phylogenetically diverse eubacterial nucleic acids against which they were tested by dot blot hybridization. (nih.gov)
  • The future envisages a DNA probe, which offers the ability to detect complex interrelationships between multiple gene expression and environmental factors, at the molecular level. (prweb.com)
  • Specific oligonucleotide probes to identify these fungi, as well as a probe to detect all dimorphic, systemic pathogens, were developed. (asm.org)
  • Then the researchers designed a microarray of more than 700 DNA probes to detect the resistant genes. (thefreedictionary.com)
  • Dr Terry Smith, of the National Diagnostics Centre at NUI Galway, said: "The Space Probe Technology uses DNA probes to detect infectious disease. (thefreedictionary.com)
  • The dna test is the most common method used to detect these infections. (healthtap.com)
  • Would a c trachomatis amp probe or a n gonorrhoeae amp probe detect trichomonasis in a male? (healthtap.com)
  • Gonorrhoeae amp probe detect trichimonasis in a male? (healthtap.com)
  • In this report, we show that several X-chromosome probes can be used for such analyses. (aacrjournals.org)
  • A grid of DNA fragments is seen, making up human chromosome 17. (sciencephoto.com)
  • These tagged DNA fragments, which may correspond to genes, have been hybridised (attached) to specific parts of chromosome 17. (sciencephoto.com)
  • A collection of Tn5-derived minitransposons has been constructed that simplifies substantially the generation of insertion mutants, in vivo fusions with reporter genes, and the introduction of foreign DNA fragments into the chromosome of a variety of gram-negative bacteria, including the enteric bacteria and typical soil bacteria like Pseudomonas species. (asm.org)
  • With the human DNA probe, signals were detected in the major sites of the ribosomal genes on chromosomes 5I (7H) and 6I (6H) only when the chromosome preparations were obtained using an optimized technique with obligatory pepsin treatment followed by hybridization. (deepdyve.com)
  • DNA nanotechnology has emerged as a promising technology for applications such as single molecule sensing, super-resolution imaging, and manipulating molecular components. (harvard.edu)
  • however, relative to natural biomolecular machines, the functional scope of DNA nanotechnology is limited by an inability to design dynamic mechanical behavior such as complex motion, conformational dynamics, or force generation. (harvard.edu)
  • The mechanical properties of DNA fundamentally constrain and enable the storage and transmission of genetic information and its use in DNA nanotechnology. (uni-muenchen.de)
  • This paper describes a new approach that used the ability of ssDNA-dsDNA probe to complex with DNA binding proteins in the solution phase and then spatially immobilized onto microarray through specific DNA hybridization. (mit.edu)
  • Finally, the partial-dsDNA microarrays were converted into a unimolecular complete-dsDNA microarray by a DNA polymerase extension reaction. (mdpi.com)
  • This unimolecular dsDNA microarray provides a general technique for high-throughput DNA-protein or DNA-drugs interactions. (mdpi.com)
  • EPA505 using a designed microarray of 8,048 probes. (sciweavers.org)
  • Burgeoning Molecular Diagnostics Market Propels the Global DNA Probes-Based Diagnostics Market, According to New Report by Global Industry Analysts, Inc. (prweb.com)
  • GIA announces the release of a comprehensive global report on DNA Probe-Based Diagnostics markets. (prweb.com)
  • The global market for DNA Probe-Based Diagnostics is forecast to reach US$30 billion by the year 2017, driven by the rapidly expanding molecular diagnostics market. (prweb.com)
  • Growth in the marketplace is also expected to stem from affluent population and widening health insurance as well as medical reimbursements, which are making expensive cutting edge diagnostic tools such as DNA-based diagnostics increasingly affordable. (prweb.com)
  • And given the need to rapidly and inexpensively determine the relevant DNA sequence information in a given organism, the potential of DNA probes-based diagnostics is huge. (prweb.com)
  • Also, the boom in the molecular diagnostics market is beginning to echo downstream into the DNA-probe based diagnostics segment. (prweb.com)
  • Further, affluent population and widening health insurance and medical reimbursements are additionally making expensive cutting edge diagnostic tools, such as DNA-based diagnostics, increasingly affordable. (prweb.com)
  • The US represents the largest regional market for DNA Probe-Based Diagnostics market worldwide, as stated by the new market research report on DNA Probes-Based Diagnostics . (prweb.com)
  • In the US, the rapid growth of DNA-based diagnostics is leading to the development of new tests for the treatment of non-contagious diseases, such as diseases related to aging or chronic diseases. (prweb.com)
  • Infectious Diseases represents the key end-use market for DNA probes-based diagnostics worldwide. (prweb.com)
  • The research report titled "DNA Probes-Based Diagnostics: A Global Strategic Business Report" announced by Global Industry Analysts Inc., provides a comprehensive review of the DNA probe-based diagnostics markets, current market trends, key growth drivers, recent product introductions, recent industry activity, and profiles of major/niche global as well as regional market participants. (prweb.com)
  • The report provides annual sales estimates and projections for DNA Probe-Based Diagnostics market for the years 2009 through 2017 for the following geographic markets - US, Canada, Japan, Europe, Asia-Pacific and Latin America. (prweb.com)
  • This econometric study covers the world outlook for DNA probes-based diagnostics across more than 200 countries. (marketresearch.com)
  • This study gives, however, my estimates for the worldwide latent demand, or the P.I.E. for DNA probes-based diagnostics. (marketresearch.com)
  • Reportlinker Adds Molecular Diagnostics Market: DNA Probes and Biochips -- New Product Development Opportunities and Business Expansion Strategies for Instrument and Reagent. (bio-medicine.org)
  • In order to successfully capitalize on the opportunities presented by the molecular diagnostics market, many companies are already exploiting new DNA probe and biochip technologies as corporate strategic assets, managed in support of business and marketing strategies. (bio-medicine.org)
  • DNA probes also render themselves as a powerful tool for chromosomal mapping of single and multiple copy genes in the haploid genome. (prweb.com)
  • Thus, this study demonstrates that physical mapping of plant chromosomes with human DNA probes that are 60 to 70% homologous to the plant genes is possible. (deepdyve.com)
  • It suggests principal opportunity for the FISH mapping of plant genomes using probes from human genome libraries, obtained in the course of the total sequencing of the human genomes and corresponding to the coding regions of genes with known functions. (deepdyve.com)
  • Specific oligonucleotide probes were developed to identify medically important fungi that display yeast-like morphology in vivo. (asm.org)
  • An immobilisation procedure based on the direct coupling of thiol-derivatised oligonucleotide probes (Probe-C6-SH) to bare gold sensor surfaces has been optimised for DNA sensing applications. (science24.com)
  • Such broadly homologous probes offer advantages over more narrowly specific probes for detecting organisms whose identity is unknown. (nih.gov)
  • Specific probes for H . capsulatum , B . dermatitidis , C . immitis , P . brasiliensis , P . marneffei , S . schenckii , C . neoformans , and P . carinii hybridized with homologous but not heterologous DNA. (asm.org)
  • You can design single stranded DNA probes using the NEBNext Custom RNA Depletion Design Tool . (neb.com)
  • Cellular proteins specifically bind to single-stranded DNA to prevent their premature recombination. (nanotech-now.com)
  • Yen Nee Tan at the A*STAR Institute of Materials Research and Engineering and co-workers1 have now developed a convenient method to characterize the interactions between single-stranded DNA and their binding proteins. (nanotech-now.com)
  • Tan and co-workers discovered that when single-stranded DNA and its binding protein were both present in the solution, coupled with a salt that stimulates nanoparticle aggregation, the DNA remained red in color, indicating that the DNA-protein complexes had bound with the nanoparticles through electrosteric stabilization forces. (nanotech-now.com)
  • In contrast, when the protein or single-stranded DNA was introduced alone in the salt solution, there was a greater shift to the blue-grey color, indicating nanoparticle aggregation (see image). (nanotech-now.com)
  • The greatest challenge in this work was to determine the optimum conditions for single-stranded DNA to bind with its binding protein to form complexes that confer the highest stability to gold nanoparticles from salt-induced aggregation," says Tan. (nanotech-now.com)
  • The binding protein can thus attach to the dissociated single-stranded DNA to form protein-DNA complexes, offering sites to which gold nanoparticles can adhere. (nanotech-now.com)
  • As they reported in Nature Communications , the team applied HiDRA to a blood cell line to test about seven million noncoding DNA fragments for ones that regulate gene expression, ultimately identifying 65,000. (broadinstitute.org)
  • An X-ray plate has been superimposed onto the grid, showing some DNA fragments tagged with a radioactive marker (dark spots). (sciencephoto.com)
  • Using primers that are 9-mer and longer and exonuclease-free enzyme results in higher labeling efficiency and longer probes. (clontech.com)
  • beta-Lactamase identification by colony hybridization with 32P-labeled DNA probes for TEM-1, SHV-1, OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 was compared with isoelectric focusing in 122 clinical isolates making a variety of enzyme types. (asm.org)
  • All strains producing a probe-type enzyme gave a positive hybridization reaction. (asm.org)
  • The report on "Global DNA Probe-based Diagnostic Market" is a professional report which provides thorough knowledge along with complete information pertaining to the DNA Probe-based Diagnostic industry a propos classifications, definitions, applications , industry chain summary, industry policies in addition to plans, product specifications, manufacturing processes, cost structures, etc. (openpr.com)
  • The Global DNA Probe-based Diagnostic Market Research Report renders deep perception of the key regional market status of the DNA Probe-based Diagnostic Industry on a global level that primarily aims the core regions which comprises of continents like Europe, North America, and Asia and the key countries such as United States, Germany, China and Japan. (openpr.com)
  • It is a methodical research depending on the market and examines the competitive framework of the global DNA Probe-based Diagnostic Market industry. (openpr.com)
  • While metal ions are known to influence DNA, little is explored on its polymer property when strong metal complexes are associated. (uwaterloo.ca)
  • EN] The invention relates to complexes that incIude lipases covalently bonded, by means of regions not involved in the active centre thereof, to biomolecules of interest (proteins such as enzymes, antibodies or proteins A or G, cofactors or DNA probes). (csic.es)
  • Dynamic DNA complexes are able to undergo multiple hybridization and dissociation events through a process called strand displacement. (rice.edu)
  • Given the ability to visualize large numbers of cellular markers using dynamic DNA probe complexes, high-content proteomic analyses can be performed on a single sample, enhancing the power of fluorescence imaging techniques. (rice.edu)
  • Furthermore, dynamic DNA complexes offer new avenues to incorporate DNA-based computations and logic for in situ molecular imaging and analyses. (rice.edu)
  • The researchers attribute binding of the nanoparticles and the DNA-protein complexes to the presence of sulphur-containing groups in the protein, which are known to create strong bonds with gold. (nanotech-now.com)
  • Enzymatic hydrolysis of the probe during PCR cycling is not required for signal release. (biosearchtech.com)
  • Furthermore, we extend the availability of orthogonal imager strands for Exchange-PAINT to over 50 and assay their orthogonality in a novel DNA origami-based crosstalk assay. (rsc.org)
  • The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. (nih.gov)
  • In a bind after a latest forensic report showed the DNA of the child delivered by a minor rape victim did not match with that of the accused, the Chandigarh Police are re-investigating the matter. (ndtv.com)
  • Here we present ethidiumbinding results for three related DNA aptamers (PDB code: 1OLD, 1DB6, and 2ARG)that bind L-argininamide (L-Arm). (mdpi.com)
  • If a laboratory today wants to test for 200 known pathogenic species, they need 200 different tests, each with its own specific DNA probe that was designed specifically to bind with DNA from a particular pathogen," said study co-author Richard Baraniuk, the lead scientist on the new study. (phys.org)
  • With compressive sensing, the disease DNA need not bind with 100 percent of the probes. (phys.org)
  • In this paper, we describe the design of probes that bind specifically to the cloning vector of DNA recombinants to facilitate physical mapping. (caltech.edu)
  • A pan-species probe was also made from the 18S ribosomal DNA that binds to DNA from all members of the complex. (ajtmh.org)
  • Research at Western Carolina University on DNA sequencing used in crime labs has gotten a $718,000 grant from the National Institute of Justice. (smokymountainnews.com)
  • Mark Wilson, director of the Forensic Science Program at WCU, has been evaluating new DNA sequencing instrumentation for use in crime laboratories. (smokymountainnews.com)
  • The grant will not only support the research but also provides scholarships for three graduate students working on related projects to help with the work, which builds on previous DNA sequencing done at WCU. (smokymountainnews.com)
  • Here we describe the analysis of sequencing data using the multiple window high-resolution DNA-SIP method (MW-HR-SIP). (springer.com)
  • This random primer DNA labeling kit is based on a modified method by Feinberg and Vogelstein and utilizes random oligonucleotide primers and cloned exonuclease-free E.coli DNA polymerase I, Klenow fragment. (clontech.com)
  • In addition, binding of RNA polymerase with promoter DNA could be detected with this strategy. (mit.edu)
  • The DNA biosensors developed have been also used to test DNA samples amplified by polymerase chain reaction (PCR). (science24.com)
  • We solve this challenge by developing molecular probes that store mechanical information. (pnas.org)
  • A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a central window opening and a registration surface against which the substrate may be mounted. (google.com)
  • In the present article, we review the occurrence and synthesis of small DNA circles, and examine their utility in studying the properties of DNA and DNA-protein interactions. (biochemsoctrans.org)
  • Exposure of mammalian cells to a variety of chemical and physical agents depresses the overal rate of DNA synthesis [1]. (springer.com)
  • R. B. Painter, Effect of caffeine on DNA synthesis in irradiated and unirradiated mammalian cells, J. Mol. (springer.com)
  • P. D. Moore, K. K. Bose, S. D. Rabdkin, and B. S. Strauss, Sites of termination of in vitro DNA synthesis on ultraviolet-and N-acetylaminofluorene-treated X174 templates by prokaryotic and eukaryotic DNA polymerases, Proc. (springer.com)
  • J. E. Cleaver, G. H. Thomas, and S. D. Park, Xeroderma pigmentosum variants have a slow recovery of DNA synthesis after irradiation with ultraviolet light, Biochim. (springer.com)
  • D. Dahle, T. D. Griffiths, and J. G. Carpenter, Inhibition and recovery of DNA synthesis in UV-irradiated Chinese hamster V-79 cells, Photochem. (springer.com)
  • S. D. Park and J. E. Cleaver, Recovery of DNA synthesis after ultraviolet irradiation of Xeroderma pigmentosum cells depends on excision repair and is blocked by caffeine, Nucleic Acids Res. (springer.com)
  • Herein, we report a universal approach for the creation of DNA-barcoded labeling probes for highly multiplexed Exchange-PAINT imaging, using a variety of affinity reagents such as primary and secondary antibodies, nanobodies, and small molecule binders. (rsc.org)
  • Now researchers at Boston University's College of Engineering, the Technical University of Munich (TUM) and Nanyang Technological University in Singapore have demonstrated a new strategy to more efficiently measure protein-induced DNA shape, or conformational changes in real-time. (bu.edu)
  • The piece of DNA that the researchers chose recognizes and binds to the BRCA1 tumor suppressor gene, mutations in which increases susceptibility to developing breast cancer. (phys.org)
  • To really understand the functional geography of the noncoding genome, however, researchers need a way to isolate and characterize thousands to millions of regulatory DNA elements within it simultaneously, rapidly, and at high resolution. (broadinstitute.org)
  • By building on these approaches, HiDRA lets researchers create massive libraries of regulatory DNA and study their influence over gene expression at nucleotide-level resolution. (broadinstitute.org)
  • In addition, the team used HiDRA to examine how disease risk DNA variants in regulatory elements affect gene expression compared to variants that do not raise risk - a boon for researchers seeking to study how minute sequence variations in promoters and enhancers can impact human traits and disease states. (broadinstitute.org)
  • Rice University researchers (clockwise from left) Richard Baraniuk, Amirali Aghazadeh and Rebekah Drezek have invented a technology that could potentially identify hundreds of bacterial pathogens simply, quickly and at low cost using a single set of random DNA probes. (phys.org)
  • Writing in the Nov. 18 Proceedings of the National Academy of Sciences (PNAS), Stanford researchers described how newly created circles of synthetic DNA - called "nanocircles" - could help researchers learn more about the aging process in cells. (innovations-report.com)
  • As in many other sensing schemes, the researchers attach DNA probes to the material's surface. (thefreedictionary.com)
  • MIT researchers, led by toxicology graduate student Yelena Margolin of the Biological Engineering Division, have discovered that the DNA damage produced by one of these inflammatory chemicals, nitrosoperoxycarbonate, occurs at unexpected locations along the DNA helix. (wordpress.com)
  • For years researchers have studied how the chemicals associated with the body's response to infection can damage DNA. (wordpress.com)
  • By using comprehensive chemical screening and analysis of the frequency of DNA damage, the researchers found that a chemical produced during inflammation, nitrosoperoxycarbonate, actually caused oxidative damage at guanines that were supposed to be the least easily oxidized. (wordpress.com)
  • The researchers used the optical properties of gold nanoparticles to probe the mechanism of protein-DNA binding. (nanotech-now.com)
  • A synthetic oligonucleotide hybridization probe comprising at least one sequence at least 12 nucleotides long, the probe forms duplexes in which at least 80% of the nucleotides are base paired with any nucleotide sequence that is either substantially of the form (Xl. (google.com.au)
  • 3. A synthetic DNA hybridization probe as defined by claim which comprises a single sequence at least 12 nucleotides long. (google.com.au)
  • 4. A locus specific synthetic DNA probe which hybridizes at high criteria to form 100% matched duplexes with a nucleotide sequence as described by claim 1. (google.com.au)
  • These three probes, plus centromeric satellite 4 (Cent4), centromeric satellite C (CentC), knob, nucleolus-organizing region (NOR), pMTY9ER telomere-associated sequence, and tandemly repeated DNA sequence 1 (TR-1) were used as a mixture for hybridization to root-tip chromosomes. (pnas.org)
  • a labeled segment of DNA or RNA used to find a specific sequence of nucleotides in a DNA molecule. (thefreedictionary.com)
  • BHQnova is most advantageous in longer probe designs, typically those over 25 bases, to boost the signal-to-noise ratio by overcoming the upper limit on sequence length. (biosearchtech.com)
  • Tan and co-workers showed that there was a minimum length of DNA sequence under which the binding protein-DNA adhesion mechanism could operate. (nanotech-now.com)
  • They found that the binding protein had a preference for binding to specific chemical units (bases) which make up DNA, and were able to spot DNA sequence variations, called single nucleotide polymorphisms (SNPs), even at the extreme ends of the molecule which are difficult to identify. (nanotech-now.com)
  • Strong fluorescence enhancement is observed when these two cyanines are bound to DNA. (spie.org)
  • The license agreement grants Ikonisys a worldwide license under Abbott patents for the manufacture and sale of DNA probes in conjunction with Chromotest(TM), a revolutionary test for the prenatal diagnosis of chromosomal abnormalities using fetal cells from the maternal circulation. (thefreedictionary.com)
  • The results indicate that the examination of respiratory tract secretions by the Gen-probe kit is a suitable screening test for the diagnosis of Legionnaires' disease. (nih.gov)
  • These probes allow us to now drill down to the DNA level and see that the cause of the disease may actually be slightly different between patients with the same diagnosis. (technologynetworks.com)
  • 2017. https://www.tabers.com/tabersonline/view/Tabers-Dictionary/733335/all/DNA_probe. (tabers.com)
  • DNA Probe-based Diagnostic Market 2017-2022, has been prepared based on an in-depth market analysis with inputs from industry experts. (openpr.com)
  • But it is likely to stir up discussions in the DNA damage and mutagenesis fields that could help us better understand the consequences of inflammation. (wordpress.com)
  • Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. (asm.org)
  • J. D. Hall and D. W. Mount, Mechanism of DNA replication and mutagenesis in ultraviolet-irradiated bacteria and mammalian cells, Prog. (springer.com)
  • photoisomerization, however, appears to be inhibited for DNA-binding by cyanine, leading to a signal-on emission. (caltech.edu)
  • Both of the cyanine probes possess a symmetric structure and bis-cationic center. (spie.org)
  • QY Research Groups has released a most recent report in view of industry research on DNA Probe-based Diagnostic market . (openpr.com)
  • The report cloaks the market analysis and projection of "DNA Probe-based Diagnostic Market" on a regional as well as global level. (openpr.com)
  • Distinguishing the increasing predominance of DNA Probe-based Diagnostic Market, this market research report demonstrates to be a key source of management and thorough data on the market across the globe. (openpr.com)
  • DNA Probe-based Diagnostic Market report helps to increase business/sales activities by understanding competitor's businesses better, company's strategic, business and operational performance, recognize potential partnerships and suppliers. (sbwire.com)
  • New York, NY -- ( SBWIRE ) -- 04/16/2018 -- The latest market intelligence study on DNA Probe-based Diagnostic market relies on statistics derived from the application of both primary and secondary research to present insights pertaining to the operational model, opportunities and competitive landscape of DNA Probe-based Diagnostic market for the forecast period, 2018 - 2025. (sbwire.com)
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  • The research also segments the DNA Probe-based Diagnostic market on the basis of the end-user, product type, application and demography for the forecast period 2018 - 2025. (sbwire.com)
  • For more clarity on the real potential of the DNA Probe-based Diagnostic market for the forecast period of 2018 - 2025 the study provides vital intelligence on the major opportunities, threats and challenges posed by the industry. (sbwire.com)
  • What are major driving factors impacting the DNA Probe-based Diagnostic market worldwide? (sbwire.com)
  • Which market trends from the yesteryears and the future are likely to keep the prospect of the DNA Probe-based Diagnostic market high for the forecast period 2018 - 2025? (sbwire.com)
  • Rice University scientists have invented a technology that could potentially identify hundreds of bacterial pathogens simply, quickly and at low cost using a single set of random DNA probes. (phys.org)
  • They are creating better ways of handling DNA, perfecting new genetic testing techniques and developing computer programs to analyze genetic variations. (latimes.com)
  • Consequently, almost half of the identifications made so far have been solely on the basis of genetic testing--682 of the 1,411 named--and DNA analysis helped in the identification of 343. (latimes.com)
  • Of the rest, there is not enough undamaged DNA to build a normal genetic profile. (latimes.com)
  • Since June, DNA experts at Celera Genomics in Rockville, Md., have processed 19,000 DNA specimens from victims and relatives, examining the genetic material contained in the thousands of mitochondria in every cell. (latimes.com)
  • People who submit DNA for ancestors testing are unwittingly becoming genetic informants on their innocent family," Mercer said, adding that they "have fewer privacy protections than convicted offenders whose DNA is contained in regulated databanks. (tampabay.com)
  • Joseph James DeAngelo, 72, was arrested Tuesday after investigators matched crime-scene DNA with genetic material stored by a distant relative on an online site. (tampabay.com)
  • That process begins with the removal of an electron from guanine, one of the four base building blocks that determine the genetic code in DNA. (wordpress.com)
  • Recombinant DNA techniques are used to isolate, reproduce, and label a portion of the genetic material, DNA, from the nucleus of a microorganism that is specific for it. (tabers.com)
  • Enzo Biochem, Inc. has announced that, as a result of a licensing agreement, it plans to enter into the cytogenetics market by producing newly patented single copy DNA probes that can identify the minutest strands of DNA, opening the door to effective treatment of genetic diseases and some cancers. (technologynetworks.com)
  • Current commercially produced DNA probes, while important and useful, are limited to primarily examining large sections of DNA in identifying relatively common genetic disorders. (technologynetworks.com)
  • Enzo also acquired rights to approximately 50 DNA probes developed at the University, which in peer review publications have been listed as being able to identify a number of genetic maladies. (technologynetworks.com)
  • An undercover investigation by the Government Accountability Office found that four genetic testing companies delivered contradictory predictions based on the same person's DNA. (nbcnews.com)
  • The agency submitted DNA samples from five staffers to four different genetic testing companies. (nbcnews.com)
  • The probes are species-specific and with the use of 32 P labeling sensitive enough so that a squash blot of only a small segment of the mosquito is required for identification. (ajtmh.org)
  • Problems in the Connecticut state police crime lab delayed for at least four years the identification of a woman investigators think was killed by suspected serial killer William Devin Howell and hindered their ability to match several different samples of DNA found in Howell's van, The Courant has learned. (courant.com)
  • Construction of a DNA probe for the specific identification of Streptococcus oralis. (asm.org)
  • Wells, Robert J. 1996-12-01 00:00:00 The random amplified polymorphic DNA (RAPD) method was investigated as a potential fish species identification method. (deepdyve.com)
  • DNA can specifically combine with ZrO 2 [ 10 ], but if DNA was directly used to fix ZrO 2 to make probes, it will be very difficult to separate the probes from the free antibodies which coexist in the suspension. (hindawi.com)
  • We also applied our DNA probes to immunofluorescence imaging using DNA-conjugated antibodies and demonstrated the ability to at least double the number of detectable markers on a single sample. (rice.edu)
  • Is it possible to hav all 3 - P24 antigen, HIV antibodies & HIV DNA undetectable at 4 MONTHS of risky exposure? (healthtap.com)
  • I didn't gel purify my PCR product, I simply removed the proteins using a Millilpore spin prep tube and precipitated the DNA. (bio.net)
  • The microscopic flexibility of DNA is a key ingredient for understanding its interaction with proteins and drugs but is still poorly understood and technically challenging to measure. (soton.ac.uk)
  • By applying a repulsive electric field at the gold surface to induce a standing orientation in the DNA probes, scientists can more easily discern and measure changes in their conformation due to IHF protein binding. (bu.edu)
  • They next introduced a DNA-binding protein and measured its impact on the intensity of the fluorophores. (bu.edu)
  • While this approach holds great promise for the bioimaging community, its widespread application has been hampered by the availability of DNA-conjugated ligands for protein labeling. (rsc.org)
  • Small DNA circles can occur in Nature, for example as protein-constrained loops, and can be synthesized by a number of methods. (biochemsoctrans.org)
  • Topological Aspects of DNA Function and Protein Folding: An Independent Meeting held at the Isaac Newton Institute for Mathematical Sciences, Cambridge, U.K., 3-7 September 2012, as part of the Isaac Newton Institute Programme Topological Dynamics in the Physical and Biological Sciences (16 July-21 December 2012). (biochemsoctrans.org)
  • Non-specific DNA, protein, surfactants and thiolated compounds cannot displace platinated DNA from GO, while non-modified DNA is easily displaced in most cases. (uwaterloo.ca)
  • Unfortunately, detailed studies of these DNA-protein interactions have been hindered by the need for expensive instrumentation and time-consuming labelling techniques. (nanotech-now.com)
  • We have studied the stacking thermodynamics of these compounds in short synthetic DNA duplexes, with all four neighboring nucleobases. (docme.ru)
  • The identity of the major glucose-utilizers was unclear as 13 C-enriched PLFA were common (16:0, 16:1, 18:1ω7, highest incorporation) and there was little difference between 12 C- and 13 C-DNA 16S rRNA gene denaturing gradient gel electrophoresis (DGGE) profiles. (wiley.com)
  • The new test uses DNA probes to pinpoint nucleotide variations that genetically distinguish among head blight species. (thefreedictionary.com)
  • Thermodynamics-guided probe design and empirical optimization of the reaction conditions have been used to enable the discrimination of single-nucleotide variants, but typically these approaches provide only an approximately 25-fold difference in binding affinity. (rice.edu)
  • In a paper online this week in Science Advances, Rice's research team used lab tests to verify that UMD could identify 11 known strains of bacteria using the same five random DNA probes. (phys.org)
  • The new study includes several computer simulations, including one that shows how a random selection of five probes can identify 40 different strains of bacteria, and another that demonstrates how the system can accurately differentiate between 24 different species of Staphylococcus. (phys.org)
  • Instead, the UMD system measures how well the disease DNA binds with each of the random probes and creates a specific binding profile for the test organism. (phys.org)
  • The work is of particular interest to faculty and scientists who are involved with the global Illumina Forensic Genomics Consortium, which is led by Wilson and top scientists specializing in the area of forensic DNA analysis. (smokymountainnews.com)
  • In an effort that rivals the Human Genome Project, Shaler has marshaled a national network that includes the New York State Police, the FBI, six biotechnology companies, a score of DNA consultants, computer software developers and an advisory committee of 30 forensic experts that has met every eight weeks to thrash out technical issues. (latimes.com)
  • Approximately 98 percent of the human genome is made up of noncoding DNA, including enhancers, promoters, and other elements that regulate gene activity. (broadinstitute.org)
  • The need is great, as more than 90 percent of variants identified in genome-wide association studies of traits and disease are located in noncoding DNA. (broadinstitute.org)
  • The paper, titled "Integrative Analysis of the Caenorhabditis elegans Genome by the modENCODE Project," was authored by Lieb and other members of the model organism ENCyclopedia Of DNA Elements ( modENCODE ) Consortium, which is funded by the National Human Genome Research Institute (NHGRI), part of the National Institutes of Health. (unc.edu)
  • It turns out that particularly important stretches of DNA in the genome are "conserved," or retained throughout evolutionary history. (unc.edu)
  • That observation overturns the prevailing theory for predicting the location of DNA damage in the genome and complicates our understanding of the basis for diseases arising from chronic inflammation," said Dedon. (wordpress.com)
  • Phosphoramidite chemistry, developed in the 1980s and later enhanced with solid-phase supports and automation, is the method of choice for DNA oligonucleotide manufacturing. (sigmaaldrich.com)
  • The sensitivity of the method was tested by using an α- 32 P-labeled DNA probe. (asm.org)
  • He will discuss their fundamental work to design and characterize nanostructures with controllable dynamic behavior and two applications that focus on implementing dynamic DNA devices to probe the structural dynamics of nucleosomes and to measure depletion forces due to molecular crowding. (harvard.edu)
  • We evaluated and optimized several DNA complex designs to function efficiently for in situ molecular analyses. (rice.edu)
  • The performances of the DNA-based sensors resulting from direct coupling of thiol-derivatised DNA probes onto gold SPR-chips or electrodes of quartz crystals, have been studied in terms of the main analytical parameters, i.e. selectivity, sensitivity, reproducibility, analysis time, etc. (science24.com)