Plants, Genetically Modified
Plant Growth Regulators
Amino Acid Sequence
Root Nodules, Plant
Volatile Organic Compounds
Medicine, African Traditional
Genetic Complementation Test
Gene Expression Profiling
Expressed Sequence Tags
Recombinant Fusion Proteins
A single limit dextrinase gene is expressed both in the developing endosperm and in germinated grains of barley. (1/4690)The single gene encoding limit dextrinase (pullulan 6-glucanohydrolase; EC 18.104.22.168) in barley (Hordeum vulgare) has 26 introns that range in size from 93 to 822 base pairs. The mature polypeptide encoded by the gene has 884 amino acid residues and a calculated molecular mass of 97,417 D. Limit dextrinase mRNA is abundant in gibberellic acid-treated aleurone layers and in germinated grain. Gibberellic acid response elements were found in the promoter region of the gene. These observations suggest that the enzyme participates in starch hydrolysis during endosperm mobilization in germinated grain. The mRNA encoding the enzyme is present at lower levels in the developing endosperm of immature grain, a location consistent with a role for limit dextrinase in starch synthesis. Enzyme activity was also detected in developing grain. The limit dextrinase has a presequence typical of transit peptides that target nascent polypeptides to amyloplasts, but this would not be expected to direct secretion of the mature enzyme from aleurone cells in germinated grain. It remains to be discovered how the enzyme is released from the aleurone and whether another enzyme, possibly of the isoamylase group, might be equally important for starch hydrolysis in germinated grain. (+info)
Characterization of Chlamydomonas reinhardtii zygote-specific cDNAs that encode novel proteins containing ankyrin repeats and WW domains. (2/4690)Genes that are expressed only in the young zygote are considered to be of great importance in the development of an isogamous green alga, Chlamydomonas reinhardtii. Clones representing the Zys3 gene were isolated from a cDNA library prepared using zygotes at 10 min after fertilization. Sequencing of Zys3 cDNA clones resulted in the isolation of two related molecular species. One of them encoded a protein that contained two kinds of protein-to-protein interaction motifs known as ankyrin repeats and WW domains. The other clone lacked the ankyrin repeats but was otherwise identical. These mRNA species began to accumulate simultaneously in cells beginning 10 min after fertilization, and reached maximum levels at about 4 h, after which time levels decreased markedly. Genomic DNA gel-blot analysis indicated that Zys3 was a single-copy gene. The Zys3 proteins exhibited parallel expression to the Zys3 mRNAs at first, appearing 2 h after mating, and reached maximum levels at more than 6 h, but persisted to at least 1 d. Immunocytochemical analysis revealed their localization in the endoplasmic reticulum, which suggests a role in the morphological changes of the endoplasmic reticulum or in the synthesis and transport of proteins to the Golgi apparatus or related vesicles. (+info)
GTPase activity and biochemical characterization of a recombinant cotton fiber annexin. (3/4690)A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation. (+info)
A multisubunit acetyl coenzyme A carboxylase from soybean. (4/4690)A multisubunit form of acetyl coenzyme A (CoA) carboxylase (ACCase) from soybean (Glycine max) was characterized. The enzyme catalyzes the formation of malonyl CoA from acetyl CoA, a rate-limiting step in fatty acid biosynthesis. The four known components that constitute plastid ACCase are biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), and the alpha- and beta-subunits of carboxyltransferase (alpha- and beta-CT). At least three different cDNAs were isolated from germinating soybean seeds that encode BC, two that encode BCCP, and four that encode alpha-CT. Whereas BC, BCCP, and alpha-CT are products of nuclear genes, the DNA that encodes soybean beta-CT is located in chloroplasts. Translation products from cDNAs for BC, BCCP, and alpha-CT were imported into isolated pea (Pisum sativum) chloroplasts and became integrated into ACCase. Edman microsequence analysis of the subunits after import permitted the identification of the amino-terminal sequence of the mature protein after removal of the transit sequences. Antibodies specific for each of the chloroplast ACCase subunits were generated against products from the cDNAs expressed in bacteria. The antibodies permitted components of ACCase to be followed during fractionation of the chloroplast stroma. Even in the presence of 0.5 M KCl, a complex that contained BC plus BCCP emerged from Sephacryl 400 with an apparent molecular mass greater than about 800 kD. A second complex, which contained alpha- and beta-CT, was also recovered from the column, and it had an apparent molecular mass of greater than about 600 kD. By mixing the two complexes together at appropriate ratios, ACCase enzymatic activity was restored. Even higher ACCase activities were recovered by mixing complexes from pea and soybean. The results demonstrate that the active form of ACCase can be reassembled and that it could form a high-molecular-mass complex. (+info)
Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation. (5/4690)We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398 amino acid residues and is predicted to have six membrane-spanning domains. To obtain evidence for the postulated function of the Arabidopsis gene, transgenic plants with the ZAT coding sequence under control of the cauliflower mosaic virus 35S promoter were analyzed. Plants obtained with ZAT in the sense orientation exhibited enhanced Zn resistance and strongly increased Zn content in the roots under high Zn exposure. Antisense mRNA-producing plants were viable, with a wild-type level of Zn resistance and content, like plants expressing a truncated coding sequence lacking the C-terminal cytoplasmic domain of the protein. The availability of ZAT can lead to a better understanding of the mechanism of Zn homeostasis and resistance in plants. (+info)
The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants. (6/4690)The Dof proteins are a large family of plant transcription factors that share a single highly conserved zinc finger. The tobacco Dof protein NtBBF1 was identified by its ability to bind to regulatory domain B in the promoter of the rolB oncogene. In this study, we show that the ACT T TA target sequence of NtBBF1 in domain B is necessary for tissue-specific expression of rolB. beta-Glucuronidase (GUS) activity of tobacco plants containing a rolB promoter-GUS fusion with a mutated NtBBF1 target sequence within domain B is almost completely suppressed in apical meristems and is severely abated in the vascular system. The ACT T TA motif is shown here also to be one of the cis-regulatory elements involved in auxin induction of rolB. The pattern of NtBBF1 expression in plants is remarkably similar to that of rolB, except in mesophyll cells of mature leaves, in which only NtBBF1 expression could be detected. Ectopic expression of rolB in mesophyll cells was achieved by particle gun delivery if the NtBBF1 binding sequence was intact. These data provide evidence that in the plant, a Dof protein DNA binding sequence acts as a transcriptional regulatory motif, and they point to NtBBF1 as the protein involved in mediating tissue-specific and auxin-inducible expression of rolB. (+info)
Isolation and characterization of SYN1, a RAD21-like gene essential for meiosis in Arabidopsis. (7/4690)The proper pairing, recombination, and segregation of chromosomes are central to meiosis and sexual reproduction. The syn1 mutation was previously identified as a synaptic mutant in a T-DNA-tagged population of plants. SYN1 has been isolated and found to exhibit similarity to Schizosaccharomyces pombe RAD21 and RAD21-like proteins, which are required for chromosome condensation and sister chromatid cohesion during mitosis. Plants homozygous for syn1 are male and female sterile and show defects in chromosome condensation and pairing beginning at leptonema of meiosis I. Fragmentation of the chromosomes was observed at metaphase I. Alternative promoters produced two SYN1 transcripts. One transcript was expressed at low levels in most tissues, whereas the other was expressed only in prebolting buds. DNA blot analyses suggest that Arabidopsis contains a small RAD21 gene family. Consistent with the DNA blot data, a second Arabidopsis RAD21-like gene has been identified. These results suggest that different RAD21-like proteins play essential roles in chromosome condensation and pairing during both meiosis and mitosis. (+info)
The FLF MADS box gene: a repressor of flowering in Arabidopsis regulated by vernalization and methylation. (8/4690)A MADS box gene, FLF (for FLOWERING LOCUS F ), isolated from a late-flowering, T-DNA-tagged Arabidopsis mutant, is a semidominant gene encoding a repressor of flowering. The FLF gene appears to integrate the vernalization-dependent and autonomous flowering pathways because its expression is regulated by genes in both pathways. The level of FLF mRNA is downregulated by vernalization and by a decrease in genomic DNA methylation, which is consistent with our previous suggestion that vernalization acts to induce flowering through changes in gene activity that are mediated through a reduction in DNA methylation. The flf-1 mutant requires a greater than normal amount of an exogenous gibberellin (GA3) to decrease flowering time compared with the wild type or with vernalization-responsive late-flowering mutants, suggesting that the FLF gene product may block the promotion of flowering by GAs. FLF maps to a region on chromosome 5 near the FLOWERING LOCUS C gene, which is a semidominant repressor of flowering in late-flowering ecotypes of Arabidopsis. (+info)
The severity of plant poisoning depends on the type of plant consumed, the amount ingested, and individual sensitivity. Some common plants that are toxic to humans include:
1. Castor bean (Ricinus communis): The seeds contain ricin, a deadly toxin that can cause severe vomiting, diarrhea, and abdominal pain.
2. Oleander (Nerium oleander): All parts of the plant are toxic, and ingestion can cause cardiac arrhythmias, seizures, and death.
3. Rhododendron (Rhododendron spp.): The leaves and flowers contain grayanotoxins, which can cause vomiting, diarrhea, and difficulty breathing.
4. Taxus (Taxus spp.): The leaves, seeds, and stems of yew (Taxus baccata) and Pacific yew (Taxus brevifolia) contain a toxin called taxine, which can cause vomiting, diarrhea, and cardiac problems.
5. Aconitum (Aconitum spp.): Also known as monkshood or wolf's bane, all parts of the plant are toxic and can cause nausea, vomiting, and abdominal pain.
6. Belladonna (Atropa belladonna): The leaves, stems, and roots contain atropine, which can cause dilated pupils, flushed skin, and difficulty urinating.
7. Deadly nightshade (Atropa belladonna): All parts of the plant are toxic and can cause nausea, vomiting, and abdominal pain.
8. Hemlock (Conium maculatum): The leaves and seeds contain coniine and gamma-coniceine, which can cause muscle weakness, paralysis, and respiratory failure.
9. Lantana (Lantana camara): The berries are toxic and can cause vomiting, diarrhea, and abdominal pain.
10. Oleander (Nerium oleander): All parts of the plant are toxic and can cause nausea, vomiting, and abdominal pain.
11. Castor bean (Ricinus communis): The seeds are particularly toxic and can cause vomiting, diarrhea, and abdominal pain.
12. Rhododendron (Rhododendron spp.): The leaves, stems, and flowers contain grayanotoxins, which can cause nausea, vomiting, and difficulty breathing.
13. Yew (Taxus spp.): The leaves, seeds, and stems of yew contain a toxin called taxine, which can cause vomiting, diarrhea, and cardiac problems.
It is important to note that while these plants are toxic, they can also be safely used in herbal remedies when prepared and administered properly under the guidance of a qualified practitioner. It is always best to consult with a medical professional before using any herbal remedy, especially if you have a medical condition or are pregnant or breastfeeding.
1. Innate immunity: This is the body's first line of defense against infection, and it involves the recognition and elimination of pathogens by cells and proteins that are present from birth.
2. Acquired immunity: This type of immunity develops over time as a result of exposure to pathogens, and it involves the production of antibodies and other immune cells that can recognize and eliminate specific pathogens.
3. Cell-mediated immunity: This is a type of immunity that involves the activation of immune cells, such as T cells and macrophages, to fight off infection.
4. Genetic resistance: Some individuals may have a genetic predisposition to disease resistance, which can be influenced by their ancestry or genetic makeup.
5. Environmental factors: Exposure to certain environmental factors, such as sunlight, clean water, and good nutrition, can also contribute to disease resistance.
Disease resistance is an important concept in the medical field, as it helps to protect against infectious diseases and can reduce the risk of illness and death. Understanding how disease resistance works can help healthcare professionals develop effective strategies for preventing and treating infections, and it can also inform public health policies and interventions aimed at reducing the burden of infectious diseases on individuals and communities.
Polyploidy is a condition where an organism has more than two sets of chromosomes, which are the thread-like structures that carry genetic information. It can occur in both plants and animals, although it is relatively rare in most species. In humans, polyploidy is extremely rare and usually occurs as a result of errors during cell division or abnormal fertilization.
In medicine, polyploidy is often used to describe certain types of cancer, such as breast cancer or colon cancer, that have extra sets of chromosomes. This can lead to the development of more aggressive and difficult-to-treat tumors.
However, not all cases of polyploidy are cancerous. Some individuals with Down syndrome, for example, have an extra copy of chromosome 21, which is a non-cancerous form of polyploidy. Additionally, some people may be born with extra copies of certain genes or chromosomal regions due to errors during embryonic development, which can lead to various health problems but are not cancerous.
Overall, the term "polyploidy" in medicine is used to describe any condition where an organism has more than two sets of chromosomes, regardless of whether it is cancerous or non-cancerous.
There are many potential causes of dehydration, including:
* Not drinking enough fluids
* Diarrhea or vomiting
* Sweating excessively
* Diabetes (when the body cannot properly regulate blood sugar levels)
* Certain medications
* Poor nutrition
* Poor sleep
To diagnose dehydration, a healthcare provider will typically perform a physical examination and ask questions about the patient's symptoms and medical history. They may also order blood tests or other diagnostic tests to rule out other conditions that may be causing the symptoms.
Treatment for dehydration usually involves drinking plenty of fluids, such as water or electrolyte-rich drinks like sports drinks. In severe cases, intravenous fluids may be necessary. If the underlying cause of the dehydration is a medical condition, such as diabetes or an infection, treatment will focus on managing that condition.
Preventing dehydration is important for maintaining good health. This can be done by:
* Drinking enough fluids throughout the day
* Avoiding caffeine and alcohol, which can act as diuretics and increase urine production
* Eating a balanced diet that includes plenty of fruits, vegetables, and whole grains
* Avoiding excessive sweating by dressing appropriately for the weather and taking breaks in cool, shaded areas when necessary
* Managing medical conditions like diabetes and kidney disease properly.
In severe cases of dehydration, complications can include seizures, organ failure, and even death. It is important to seek medical attention if symptoms persist or worsen over time.
DNA Plant Technology
Plant DNA C-values Database
RNA-directed DNA methylation
DNA adenine methyltransferase identification
T7 DNA helicase
Decrease in DNA Methylation I (DDM1)
Pollen DNA barcoding
DNA repair protein XRCC4
Φ29 DNA polymerase
DNA evidence in the O. J. Simpson murder case
Algae DNA barcoding
Fungal DNA barcoding
Transfer DNA binary system
DNA barcoding in diet assessment
Crosslinking of DNA
Immortal DNA strand hypothesis
NCIS backdoor pilot
Dryandra ser. Aphragma
2022 monkeypox outbreak
Mopeds India Limited
Prunus × nudiflora
Stolen (2012 film)
1972 in the Vietnam War
History of Madeira
Maxwell® RSC Plant DNA Kit
DNA extraction machine for plant breeding | Festo GB
Taxonomy browser (plant/fungus mixed DNA library)
Environmental DNA surveillance of biocontamination in a drinking water treatment plant - PubMed
Control of DNA recombination in plants | Research Institute of Molecular Pathology (IMP)
APPLICATION OF DNA TECHNOLOGY IN BREEDING PROTEACEOUS PLANTS | International Society for Horticultural Science
Evaluation of the uniformity and stability of T-DNA integration and gene expression in transgenic apple plants | Flachowsky |...
Munin: Sedimentary ancient DNA shows terrestrial plant richness continuously increased over the Holocene in northern...
AceMag™ Plant DNA Kit|AcceGen
plant dna - Very Cool Writing...
Grip op DNA - Plant en Zo
Magnetic Plant Genomic DNA Kit - NEOMEDSCIENTIFIC
Heavy reliance on plants for Romanian cave bears evidenced by amino acid nitrogen isotope analysis | Scientific Reports
Plant RNA/DNA Purification Kit - PlantMedia
Genomic DNA Extraction by Sample Type | Thermo Fisher Scientific - US
Diversity of plant DNA in stool is linked to dietary quality, age, and household income. | Proc Natl Acad Sci U S A;120(27):...
Saudi Kayan receives four bids in plant retender
dna study slavery - PeanutDB Plant Genomic sequence Database
DNA: The Essence of What is Being Planted
DNA barcoding to promote social awareness and identity of neglected, underutilized plant species having valuable nutritional...
Woman whose rape DNA led to her arrest to sue San Francisco - The San Diego Union-Tribune
Equipment And Reagents For Dna Extraction Of Plant Materials
DNA Sequence Variation among Conspecific Accessions of the Legume...: Ingenta Connect
SARS-CoV-2 suppresses mRNA expression of selenoproteins associated with ferroptosis, endoplasmic reticulum stress and DNA...
Plant Systematics | Arun K. Pandey, Shruti Kasana | Taylor & Francis e
Animal and plant DNA extraction kits and services | LGC Biosearch Technologies
Horizontal DNA transfer in Erythronium dens-canis - CNRGV : Plant Genomic Center
- The A and B DNA components of each virus have cis-acting sequences necessary for replication, and their A components encode trans-acting factors are required for this process. (ncsu.edu)
- A DNA sequence required for geminivirus replication also mediates transcriptional regulation. (ncsu.edu)
- Tomato golden mosaic virus (TGMV), a member of the geminivirus family, requires a single virus-encoded protein for DNA replication. (ncsu.edu)
- These experiments revealed that the repeated motifs in the AL1 binding site contribute differentially to repression, as has been observed previously for AL1-DNA binding and viral replication. (ncsu.edu)
- Interaction between a geminivirus replication protein and origin DNA is essential for viral replication. (ncsu.edu)
- Ciprofloxacin hydrochloride and Norfloxacin are second-generation fluoroquinolone antibiotic in opposition to bacterial DNA gyrase, which reduces DNA pressure all through replication. (plant-gem.org)
- The biphasic interphase-mitotic polarity of cell nuclei induced under DNA replication stress seems to be correlated with Pin2 localization in root meristems of Allium cepa. (nih.gov)
- Dissimilar effects of β-lapachone- and hydroxyurea-induced DNA replication stress in root meristem cells of Allium cepa. (nih.gov)
- This indicates that even in the absence of DNA replication, a distinct subset of IR-induced damage is recognized by ATR. (nih.gov)
Automated DNA purification1
- Our portfolio of high-performance DNA and RNA extraction and purifications kits, automated DNA purification instruments, and tailor-made extraction services deliver high-quality DNA and RNA for downstream sequencing and PCR applications enabling you to make critical research decisions and advance traits to market. (azurewebsites.net)
- The generation of transgenic apple plants relies on the molecular analysis of transgene integration and expression based on polymerase chain reaction (PCR) analysis, blotting techniques and enzymatic assays on vitro leaves of putative transgenic regenerates. (ejbiotechnology.info)
- Plants were evaluated using standard molecular techniques, such as PCR, Southern blot, reverse transcription PCR (RT-PCR) and Enzyme Linked Immunosorbent Assay (ELISA), and propagated in vitro on non-selective antibiotic-free media for four years to mimic natural conditions in the field. (ejbiotechnology.info)
- The MasterPure Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types. (azurewebsites.net)
- To prove the phenomenon was real, a combination of genomic, molecular and cytogenetic analyses were performed to confirm the exogenous origin of the DNA considered. (inrae.fr)
- This project encompasses many basic plant systematic activities: plant collection and herbarium curation, DNA extraction and PCR amplification, molecular sequence and phylogenetic analysis. (csustan.edu)
- Here, we develop a molecular tool to quantify human dietary plant diversity by applying DNA metabarcoding with the chloroplast trnL-P6 marker to 1,029 fecal samples from 324 participants across two interventional feeding studies and three observational cohorts. (bvsalud.org)
- Obtaining high-quality, intact DNA is often the first and most critical step in many fundamental molecular biology applications, such as DNA cloning , sequencing , PCR , and electrophoresis . (thermofisher.com)
- DNA extraction from cells is one of the most widely used molecular biology techniques. (thermofisher.com)
- Plant Physiol Biochem. (nih.gov)
- In fact, the researchers found single-stranded DNA rings that originated in viruses, which they named bovine meat and milk factors (BMMF), in the intestines of patients with colon cancer. (medscape.com)
- Dr. Nabel and his colleagues previously have shown the DNA vaccine approach to be effective against influenza viruses in animal models, including highly pathogenic viruses such as the H5N1 strain and the H1N1 virus that caused the deadly 1918 pandemic. (nih.gov)
- The DNA vaccine used in this study is similar to other investigational vaccines evaluated by the VRC that hold promise for controlling other viruses, such as HIV, Ebola, SARS and West Nile. (nih.gov)
- With this study, the investigators hope to learn whether new technologies, such as DNA vaccines, can provide protection against such viruses. (nih.gov)
- Papillomaviruses, as well as human herpes and Epstein-Barr viruses (EBV), polyomaviruses, and retroviruses, cause cancer in a direct way: by inserting their genes into the DNA of human cells. (medscape.com)
- Among these analyses, the isolation of the ribosomal DNA genomic sequence from Potentilla from the genome of Erythronium dens-canis was performed at the CNRGV. (inrae.fr)
- For example, many DNA extraction methods will work equally well for tissues such as liver, but fibrous tissues such as heart, fatty tissues such as brain, and nuclease-rich tissues like spleen present challenges for DNA isolation. (thermofisher.com)
- Following efficient homogenization using a SpeedMill, other homogenizer, or a mortar and liquid nitrogen, the plant material is lysed, and proteins and polysaccharides are effectively removed in a single precipitation step. (mirtas-zuckertraum.de)
- Because of the high content of the secondary metabolites, proteins, polysaccharides and polyphenolic compounds into the plant cell CTAB DNA extraction buffer is the first choice in the plant DNA extraction. (mirtas-zuckertraum.de)
- Once inside the body, the DNA instructs human cells to make proteins that act as a vaccine against the virus. (nih.gov)
- In the lab, TAL effectors can be fused with DNA-breaking proteins called nucleases. (nih.gov)
- CTAB DNA extraction buffer is more suitable for extracting DNA from the plant tissues. (mirtas-zuckertraum.de)
- Analysis of protein extracts from healthy plants and plants infected with TGMV by SDS-PAGE and immunoblotting showed that a protein corresponding to the predicted AL3 gene product is produced only in infected plants. (ncsu.edu)
- 22. Impact of phytochemicals and plant extracts on viability and proliferation of NK cell line NK-92 - a closer look at immunomodulatory properties of goji berries extract in human colon cancer cells. (nih.gov)
- Using cells in the lab, the investigators designed mitoTALENs to bind and cut mitochondrial DNA that had a specific mutation in the gene Complex I, which causes LHON. (nih.gov)
- Teaches BIOL 1150: General Biology II, BOTY 1010: Plant Biology for non-majors, BOTY 3700: Flowering plants, and BOTY 3130: Plant, algae, and fungi morphology. (csustan.edu)
- Ancient algae New scanning technology gives scientists an extraordinary view inside the cells of what may be 1.6 billion-year-old red algae, the oldest plant-like fossils ever found. (abc.net.au)
- Since the development of a reliable DNA extraction method on ten genera of Proteaceae in 1994 by Maguire et al. (ishs.org)
- The Stanislaus State Herbarium houses and catalogs thousands of plant specimens, with more added regularly. (csustan.edu)
- It is estimated that about 7000 plant species and a large number of cultivars and varieties have been cultivated for consumption in human history. (unimib.it)
- According to this letter of intent, DNA Genetics and Segra will transition a set of existing DNA Genetics cannabis cultivars into tissue culture for genetic preservation and production purposes. (segra-intl.com)
- Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone. (nature.com)
- In order to assess the uniformity and the stability of transfer DNA (T-DNA) integration and gene expression, we studied 26 transgenic apple lines carrying the attacin E gene from Hyalophora cecropia , the β-glucuronidase gene, and the nptII gene. (ejbiotechnology.info)
- These data reveal the transitory nature of telomere stability, and the robust and flexible nature of DNA repair mechanisms elicited by telomere dysfunction. (nih.gov)
- Viral DNA extraction poses unique challenges for good recovery and detection sensitivity. (thermofisher.com)
- We have developed DNA purification products that are optimized to provide maximum viral DNA yield, purity, and integrity from a broad range of sample types in several format options. (thermofisher.com)
- Which kit for viral DNA extraction is right for you? (thermofisher.com)
- The chemistry underlying the innuPREP Plant RNA Kit, which has been specially adapted for isolating plant materials ( leaves, caulis, root, blossom), guarantees highly efficient lysis and effectively deactivates endogenous and exogenous RNases. (mirtas-zuckertraum.de)
- The research and application can be divided into the following areas: 1) DNA diversity studies for parent selection in breeding programmes, for systematics and for ecological characterisation. (ishs.org)
- The quality of DNA produced from this method needed to be high enough for downstream PCRbased genetic analysis. (mirtas-zuckertraum.de)
- Pairing the oKtopure system with sbeadex chemistry provides an industry-leading solution driving laboratory efficiency and obtaining high quality and high-throughput DNA extraction and purification to feed downstream NGS, microarray and PCR workflows. (azurewebsites.net)
- The instrument delivers high quality DNA suitable for downstream applications such as sequencing, microarrays and SNP genotyping. (azurewebsites.net)
- Whether you prefer organic reagents, filter columns, or magnetic beads, our DNA purification products are designed for sensitive, scalable extraction and are compatible with a range of downstream applications. (thermofisher.com)
- Rapid genomic and phenotypic change in response to climate warming Predicting the distribution of plants under climate change constrained by our limited understanding of the potential for rapid adaptive evolution. (bioinfolab.org)
- The aim of our study was to develop a rapid and cost efficient method for extraction of genomic DNA from fresh leaves of Zea mays and dry leaves of Anacardium occidentale. (mirtas-zuckertraum.de)
- The QuickExtract™ Plant DNA Extraction Solution provides a rapid, inexpensive method for isolating PCR-ready DNA from plant tissue and other sample types, making this kit ideal for processing hundreds of samples simultaneously. (azurewebsites.net)
- Our research is currently centered on two projects: A DNA barcode-based community phylogeny of our campus plants and developing methods to quantify Downingia floral shape to allow us to reconstruct floral evolution with the group. (csustan.edu)
- Plant DNA purification is fundamentally more complex, due to the nature of plant tissue, which includes tough cell walls, complex polysaccharides, and other compounds that may affect DNA quality. (mirtas-zuckertraum.de)
- Using the innuPREP Plant DNA KitIPC16 allows users to isolate highly pure genomic DNA from a variety of plant materials. (mirtas-zuckertraum.de)
- يمكنك الحصول على سعر Equipment And Reagents For Dna Extraction Of Plant Materials وسيتصل بك ممثل A&C في غضون يوم عمل واحد. (mirtas-zuckertraum.de)
- Many commercial kits and reagents are now available, specifically designed to tackle the plant tissue for fast and efficient extraction of DNA. (mirtas-zuckertraum.de)
- Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles. (nature.com)
- Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture. (nature.com)
- The oKtopure™ automated DNA extraction and purification system is a platform which combines standardised high-throughput DNA extraction from plant tissue with our proprietary sbeadex magnetic bead based extraction chemistry. (azurewebsites.net)
- The oKtopure instrument uses the proprietary sbeadex magnetic bead based chemistry to perform automated DNA extraction from 8 x 96-well plates in parallel. (azurewebsites.net)
- In the next several decades, scientists would learn more about genes and the special substance called DNA that carried each living thing's specific traits. (nih.gov)
- An aging power plant in St. Petersburg's Kolpino district, which encompasses more than 30 large industrial and manufacturing companies, has been unable to meet the area's constantly growing demand for power. (eponline.com)
- This paper describes a method for DNA extraction from symptomatic plant material in the field and the use of a portable realtime PCR platform (Cepheid SmartCycler) for accurate onsite detection of P. ramorum within 2 h. (mirtas-zuckertraum.de)
- The absence of translational regulation of ferritin in plants contrasts with ferritin regulation in animals. (ncsu.edu)
- During analyses of the newly sequenced genome of Erythronium dens-canis , the presence of a horizontal transfer of ribosomal DNA from Potentilla, an eudicot plant of the Rosaceae family, to the genome of Erythronium dens-canis , a monocot plant of the Liliaceae family, was observed. (inrae.fr)
- For this purpose, we constructed a BAC library from the nuclei of the Erythronium dens-canis genome sorted by flow cytometry by the Centre of Plant Structural and Functional Genomics - Czech Republic , a method allowing to eliminate all cytoplasmic ribosomal DNA contaminations. (inrae.fr)
- The process that takes place in the machine is used in the agricultural industry for plant breeding. (festo.com)
- Publicly available and validated DNA reference sequences useful for phylogeny estimation and identification of fungal pathogens are an increasingly important resource in the efforts of plant protection organizations to facilitate safe international trade of agricultural commodities. (nih.gov)
- This phosphorylated H2AX (gamma-H2AX) is involved in the retention of repair and signaling factor complexes at sites of DNA damage. (nih.gov)
- We assessed the impact of Holocene climate changes and nutrients on terrestrial plant richness in northern Fennoscandia. (uit.no)
- The number of plant taxa per sample ( plant metabarcoding richness or pMR) correlated with recorded intakes in interventional diets and with indices calculated from a food frequency questionnaire in typical diets (ρ = 0.40 to 0.63). (bvsalud.org)
- Climate sensitivity The plant life of Australia's outback does not respond to sudden increases in rainfall because it has 'learned' that drought will soon follow, according to satellite-based maps tracking the impact of changing climatic conditions. (abc.net.au)
- Polymerase chain reaction: locates and makes copies of parts of the DNA contained in the castor bean plant. (cdc.gov)
- differences were also found between distinct plants of a single T-line. (ejbiotechnology.info)
- Achieve greater flexibility and efficiency with our DNA and RNA extraction services. (azurewebsites.net)
- To support this effort and to promote projects like the Kolpino power plant, the Russian government has launched initiatives for energy infrastructure investment and energy efficiency," said Ruslan Pakhomov, GE Energy executive director, Russia. (eponline.com)
- GE's gas turbine technology has been selected for a power plant upgrade that will help meet the energy needs of a rapidly growing industrial district in St. Petersburg, the capital of Russia's northwest region. (eponline.com)
- The histone variant H2AX is rapidly phosphorylated at the sites of DNA double-strand breaks (DSBs). (nih.gov)
- Sequence analysis revealed that, relative to tert ku70, chromosome fusion junctions in tert ku70 lig4 mutants contained less microhomology and less telomeric DNA. (nih.gov)
- Scientists recently began using TAL effectors to modify DNA in a variety of organisms. (nih.gov)
- DNA Genetics is interested in utilizing micropropagated plantlets to further optimize its production capability, preserve genetics, and reduce the pathogen risks associated with traditional cannabis nursery operations. (segra-intl.com)
- DNA Genetics is a world-leading supplier of the highest quality cannabis seeds for the medicinal and recreational markets. (segra-intl.com)
- Established over thirteen years ago in Amsterdam, DNA Genetics has developed an exceptional global cannabis brand. (segra-intl.com)
- Winners of numerous awards at The High Times Cannabis Cup, Emerald Cup, Spannabis, Chalice, and ExpoWeed, DNA Genetics has a proven track record of driving product trends in the global marketplace. (segra-intl.com)
- As a result, customers in the seed industry can collect DNA from up to 40,000 samples per day. (festo.com)
- In adolescents unable to collect validated dietary survey data, trnL metabarcoding detected 111 plant taxa, with 86 consumed by more than one individual and four ( wheat , chocolate , corn , and potato family ) consumed by >70% of individuals. (bvsalud.org)
- We have expertise in extracting nucleic acid material from over 100 different species and tissue types, and our scientists are ready to work with you on obtaining high quality DNA extractions from your toughest samples. (azurewebsites.net)
- The first human trial of a DNA vaccine designed to prevent H5N1 avian influenza infection began on December 21, 2006, when the vaccine was administered to the first volunteer at the National Institutes of Health (NIH) Clinical Center in Bethesda, MD. Scientists from the Vaccine Research Center (VRC) at the National Institute of Allergy and Infectious Diseases (NIAID), one of the NIH Institutes, designed the vaccine. (nih.gov)
- We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif. (nature.com)
- The search specifically identifies the DNA of the gene that produces the ricin protein. (cdc.gov)
- We tested the air for chemicals commonly found in surgical smoke produced by What Morton Plant Hospital Managers electrocautery knives during surgery. (cdc.gov)
- DNA fingerprinting was used to evaluate epidemiologically linked case pairs found during routine tuberculosis (TB) contact investigations in seven sentinel sites from 1996 to 2000. (cdc.gov)
- In nature, TAL effectors are found only in certain types of plant-infecting bacteria. (nih.gov)
- It is found in water, soil, and air, and is a source of much needed nitrogen for plants and animals. (cdc.gov)
- DIY killers Carnivorous plants around the world all developed their killer habit in surprisingly similar fashion, according to a genetic study of distantly related pitcher plants from Australia, Asia and America. (abc.net.au)
- This publication describes a unique example of horizontal DNA transfer from nucleus to nucleus between non-parasitic eudicot and monocot. (inrae.fr)
- They enable the bacteria to use plant DNA to multiply and spread infection. (nih.gov)