PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)
Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.
The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990)
The functional hereditary units of PLANTS.
Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.
New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.
Plants whose roots, leaves, seeds, bark, or other constituent parts possess therapeutic, tonic, purgative, curative or other pharmacologic attributes, when administered to man or animals.
Deoxyribonucleic acid that makes up the genetic material of plants.
Processes orchestrated or driven by a plethora of genes, plant hormones, and inherent biological timing mechanisms facilitated by secondary molecules, which result in the systematic transformation of plants and plant parts, from one stage of maturity to another.
Plants or plant parts which are harmful to man or other animals.
Basic functional unit of plants.
Parts of plants that usually grow vertically upwards towards the light and support the leaves, buds, and reproductive structures. (From Concise Dictionary of Biology, 1990)
The genetic complement of a plant (PLANTS) as represented in its DNA.
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
An organism of the vegetable kingdom suitable by nature for use as a food, especially by human beings. Not all parts of any given plant are edible but all parts of edible plants have been known to figure as raw or cooked food: leaves, roots, tubers, stems, seeds, buds, fruits, and flowers. The most commonly edible parts of plants are FRUIT, usually sweet, fleshy, and succulent. Most edible plants are commonly cultivated for their nutritional value and are referred to as VEGETABLES.
The parts of plants, including SEEDS.
Any of the hormones produced naturally in plants and active in controlling growth and other functions. There are three primary classes: auxins, cytokinins, and gibberellins.
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
The inherent or induced capacity of plants to withstand or ward off biological attack by pathogens.
A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; its dried leaves are used for SMOKING.
A thin layer of cells forming the outer integument of seed plants and ferns. (Random House Unabridged Dictionary, 2d ed)
Closable openings in the epidermis of plants on the underside of leaves. They allow the exchange of gases between the internal tissues of the plant and the outside atmosphere.
Members of the group of vascular plants which bear flowers. They are differentiated from GYMNOSPERMS by their production of seeds within a closed chamber (OVARY, PLANT). The Angiosperms division is composed of two classes, the monocotyledons (Liliopsida) and dicotyledons (Magnoliopsida). Angiosperms represent approximately 80% of all known living plants.
The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.
Poisoning by the ingestion of plants or its leaves, berries, roots or stalks. The manifestations in both humans and animals vary in severity from mild to life threatening. In animals, especially domestic animals, it is usually the result of ingesting moldy or fermented forage.
The loss of water vapor by plants to the atmosphere. It occurs mainly from the leaves through pores (stomata) whose primary function is gas exchange. The water is replaced by a continuous column of water moving upwards from the roots within the xylem vessels. (Concise Dictionary of Biology, 1990)
The relationships of groups of organisms as reflected by their genetic makeup.
A plant species of the family SOLANACEAE, native of South America, widely cultivated for their edible, fleshy, usually red fruit.
The reproductive organs of plants.
A localized proliferation of plant tissue forming a swelling or outgrowth, commonly with a characteristic shape and unlike any organ of the normal plant. Plant tumors or galls usually form in response to the action of a pathogen or a pest. (Holliday, P., A Dictionary of Plant Pathology, 1989, p330)
Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.
The above-ground plant without the roots.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Material prepared from plants.
A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER.
Very young plant after GERMINATION of SEEDS.
The act of feeding on plants by animals.
Units that convert some other form of energy into electrical energy.
Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.
The large family of plants characterized by pods. Some are edible and some cause LATHYRISM or FAVISM and other forms of poisoning. Other species yield useful materials like gums from ACACIA and various LECTINS like PHYTOHEMAGGLUTININS from PHASEOLUS. Many of them harbor NITROGEN FIXATION bacteria on their roots. Many but not all species of "beans" belong to this family.
Sugar-rich liquid produced in plant glands called nectaries. It is either produced in flowers or other plant structures, providing a source of attraction for pollinating insects and animals, as well as being a nutrient source to animal mutualists which provide protection of plants against herbivores.
Eighteen-carbon cyclopentyl polyunsaturated fatty acids derived from ALPHA-LINOLENIC ACID via an oxidative pathway analogous to the EICOSANOIDS in animals. Biosynthesis is inhibited by SALICYLATES. A key member, jasmonic acid of PLANTS, plays a similar role to ARACHIDONIC ACID in animals.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The outer layer of the woody parts of plants.
The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.
The synthesis by organisms of organic chemical compounds, especially carbohydrates, from carbon dioxide using energy obtained from light rather than from the oxidation of chemical compounds. Photosynthesis comprises two separate processes: the light reactions and the dark reactions. In higher plants; GREEN ALGAE; and CYANOBACTERIA; NADPH and ATP formed by the light reactions drive the dark reactions which result in the fixation of carbon dioxide. (from Oxford Dictionary of Biochemistry and Molecular Biology, 2001)
Physiological functions characteristic of plants.
A group of alicyclic hydrocarbons with the general formula R-C5H9.
Acetic acid derivatives of the heterocyclic compound indole. (Merck Index, 11th ed)
A plant species of the genus SOLANUM, family SOLANACEAE. The starchy roots are used as food. SOLANINE is found in green parts.
The unconsolidated mineral or organic matter on the surface of the earth that serves as a natural medium for the growth of land plants.
Systems of medicine based on cultural beliefs and practices handed down from generation to generation. The concept includes mystical and magical rituals (SPIRITUAL THERAPIES); PHYTOTHERAPY; and other treatments which may not be explained by modern medicine.
Substances released by PLANTS such as PLANT GUMS and PLANT RESINS.
A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)
A compound obtained from the bark of the white willow and wintergreen leaves. It has bacteriostatic, fungicidal, and keratolytic actions.
Knobbed structures formed from and attached to plant roots, especially of LEGUMES, which result from symbiotic infection by nitrogen fixing bacteria such as RHIZOBIUM or FRANKIA. Root nodules are structures related to MYCORRHIZAE formed by symbiotic associations with fungi.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
The reproductive cells of plants.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Symbiotic combination (dual organism) of the MYCELIUM of FUNGI with the roots of plants (PLANT ROOTS). The roots of almost all higher plants exhibit this mutually beneficial relationship, whereby the fungus supplies water and mineral salts to the plant, and the plant supplies CARBOHYDRATES to the fungus. There are two major types of mycorrhizae: ectomycorrhizae and endomycorrhizae.
Use of plants or herbs to treat diseases or to alleviate pain.
A large family of narrow-leaved herbaceous grasses of the order Cyperales, subclass Commelinidae, class Liliopsida (monocotyledons). Food grains (EDIBLE GRAIN) come from members of this family. RHINITIS, ALLERGIC, SEASONAL can be induced by POLLEN of many of the grasses.
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
The failure of PLANTS to complete fertilization and obtain seed (SEEDS) as a result of defective POLLEN or ovules, or other aberrations. (Dict. of Plant Genet. and Mol. Biol., 1998)
The fertilizing element of plants that contains the male GAMETOPHYTES.
A plant genus of the family Cruciferae. It contains many species and cultivars used as food including cabbage, cauliflower, broccoli, Brussel sprouts, kale, collard greens, MUSTARD PLANT; (B. alba, B. junica, and B. nigra), turnips (BRASSICA NAPUS) and rapeseed (BRASSICA RAPA).
The initial stages of the growth of SEEDS into a SEEDLINGS. The embryonic shoot (plumule) and embryonic PLANT ROOTS (radicle) emerge and grow upwards and downwards respectively. Food reserves for germination come from endosperm tissue within the seed and/or from the seed leaves (COTYLEDON). (Concise Dictionary of Biology, 1990)
A large plant family of the order Asterales, subclass Asteridae, class Magnoliopsida. The family is also known as Compositae. Flower petals are joined near the base and stamens alternate with the corolla lobes. The common name of "daisy" refers to several genera of this family including Aster; CHRYSANTHEMUM; RUDBECKIA; TANACETUM.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Oils derived from plants or plant products.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Prolonged dry periods in natural climate cycle. They are slow-onset phenomena caused by rainfall deficit combined with other predisposing factors.
The study of the origin, structure, development, growth, function, genetics, and reproduction of plants.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
A variable annual leguminous vine (Pisum sativum) that is cultivated for its rounded smooth or wrinkled edible protein-rich seeds, the seed of the pea, and the immature pods with their included seeds. (From Webster's New Collegiate Dictionary, 1973)
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.
Abscission-accelerating plant growth substance isolated from young cotton fruit, leaves of sycamore, birch, and other plants, and from potatoes, lemons, avocados, and other fruits.
A class of organic compounds known as STEROLS or STEROIDS derived from plants.
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
A plant genus of the family POACEAE. The EDIBLE GRAIN, barley, is widely used as food.
A species of gram-negative, fluorescent, phytopathogenic bacteria in the genus PSEUDOMONAS. It is differentiated into approximately 50 pathovars with different plant pathogenicities and host specificities.
A class of plants within the Bryophyta comprising the mosses, which are found in both damp (including freshwater) and drier situations. Mosses possess erect or prostrate leafless stems, which give rise to leafless stalks bearing capsules. Spores formed in the capsules are released and grow to produce new plants. (Concise Dictionary of Biology, 1990). Many small plants bearing the name moss are in fact not mosses. The "moss" found on the north side of trees is actually a green alga (CHLOROPHYTA). Irish moss is really a red alga (RHODOPHYTA). Beard lichen (beard moss), Iceland moss, oak moss, and reindeer moss are actually LICHENS. Spanish moss is a common name for both LICHENS and an air plant (TILLANDSIA usneoides) of the pineapple family. Club moss is an evergreen herb of the family LYCOPODIACEAE.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
The capacity of an organism to defend itself against pathological processes or the agents of those processes. This most often involves innate immunity whereby the organism responds to pathogens in a generic way. The term disease resistance is used most frequently when referring to plants.
The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
Porphyrin derivatives containing magnesium that act to convert light energy in photosynthetic organisms.
A group of plant cells that are capable of dividing infinitely and whose main function is the production of new growth at the growing tip of a root or stem. (From Concise Dictionary of Biology, 1990)
An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.
Self-replicating cytoplasmic organelles of plant and algal cells that contain pigments and may synthesize and accumulate various substances. PLASTID GENOMES are used in phylogenetic studies.
A family (Aphididae) of small insects, in the suborder Sternorrhyncha, that suck the juices of plants. Important genera include Schizaphis and Myzus. The latter is known to carry more than 100 virus diseases between plants.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
Woody, usually tall, perennial higher plants (Angiosperms, Gymnosperms, and some Pterophyta) having usually a main stem and numerous branches.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Organic compounds that have a relatively high VAPOR PRESSURE at room temperature.
A creeping annual plant species of the CUCURBITACEAE family. It has a rough succulent, trailing stem and hairy leaves with three to five pointed lobes.
Plant hormones that promote the separation of daughter cells after mitotic division of a parent cell. Frequently they are purine derivatives.
A technique for growing plants in culture solutions rather than in soil. The roots are immersed in an aerated solution containing the correct proportions of essential mineral salts. (From Concise Dictionary of Biology, 1990)
Any of several BRASSICA species that are commonly called mustard. Brassica alba is white mustard, B. juncea is brown or Chinese mustard, and B. nigra is black, brown, or red mustard. The plant is grown both for mustard seed from which oil is extracted or used as SPICES, and for its greens used as VEGETABLES or ANIMAL FEED. There is no relationship to MUSTARD COMPOUNDS.
The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions.
A large order of insects characterized by having the mouth parts adapted to piercing or sucking. It is comprised of four suborders: HETEROPTERA, Auchenorrhyncha, Sternorrhyncha, and Coleorrhyncha.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
An endosymbiont that is either a bacterium or fungus living part of its life in a plant. Endophytes can benefit host plants by preventing pathogenic organisms from colonizing them.
The variety of all native living organisms and their various forms and interrelationships.
A plant family of the order Solanales, subclass Asteridae. Among the most important are POTATOES; TOMATOES; CAPSICUM (green and red peppers); TOBACCO; and BELLADONNA.
The non-genetic biological changes of an organism in response to challenges in its ENVIRONMENT.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A division of the plant kingdom. Bryophyta contains the subdivision, Musci, which contains the classes: Andreaeopsida, BRYOPSIDA, and SPHAGNOPSIDA.
Seedless nonflowering plants of the class Filicinae. They reproduce by spores that appear as dots on the underside of feathery fronds. In earlier classifications the Pteridophyta included the club mosses, horsetails, ferns, and various fossil groups. In more recent classifications, pteridophytes and spermatophytes (seed-bearing plants) are classified in the Subkingdom Tracheobionta (also known as Tracheophyta).
The physical distribution of plants in various forms and stages of development through time and space.
A phylum of fungi which have cross-walls or septa in the mycelium. The perfect state is characterized by the formation of a saclike cell (ascus) containing ascospores. Most pathogenic fungi with a known perfect state belong to this phylum.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Plant tissue that carries water up the root and stem. Xylem cell walls derive most of their strength from LIGNIN. The vessels are similar to PHLOEM sieve tubes but lack companion cells and do not have perforated sides and pores.
A class of plant growth hormone isolated from cultures of Gibberella fujikuroi, a fungus causing Bakanae disease in rice. There are many different members of the family as well as mixtures of multiple members; all are diterpenoid acids based on the gibberellane skeleton.
A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.
Gymnosperms are a group of vascular plants whose seeds are not enclosed by a ripened ovary (fruit), in contrast to ANGIOSPERMS whose seeds are surrounded by an ovary wall. The seeds of many gymnosperms (literally, "naked seed") are borne in cones and are not visible. Taxonomists now recognize four distinct divisions of extant gymnospermous plants (CONIFEROPHYTA; CYCADOPHYTA; GINKGOPHYTA; and GNETOPHYTA).
Facilities that convert NUCLEAR ENERGY into electrical energy.
The fleshy or dry ripened ovary of a plant, enclosing the seed or seeds.
The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.
A plant genus of the family Plantaginaceae. The small plants usually have a dense tuft of basal leaves and long, leafless stalks bearing a terminal spike of small flowers. The seeds, known as PSYLLIUM, swell in water and are used as laxatives. The leaves have been used medicinally.
A system of traditional medicine which is based on the beliefs and practices of the African peoples. It includes treatment by medicinal plants and other materia medica as well as by the ministrations of diviners, medicine men, witch doctors, and sorcerers.
A plant genus in the family LILIACEAE (sometimes placed in Asparagaceae) that contains ECDYSTEROIDS and is an ingredient of Siotone. The shoots are used as a vegetable and the roots are used in FOLK MEDICINE.
A part of the embryo in a seed plant. The number of cotyledons is an important feature in classifying plants. In seeds without an endosperm, they store food which is used in germination. In some plants, they emerge above the soil surface and become the first photosynthetic leaves. (From Concise Dictionary of Biology, 1990)
The mint plant family. They are characteristically aromatic, and many of them are cultivated for their oils. Most have square stems, opposite leaves, and two-lipped, open-mouthed, tubular corollas (united petals), with five-lobed, bell-like calyxes (united sepals).
The study of the actions and properties of medicinal agents, often derived from PLANTS, indigenous to populations or ETHNIC GROUPS.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
A plant genus of the family CUCURBITACEAE, order Violales, subclass Dilleniidae, which includes pumpkin, gourd and squash.
A genus herbs of the Asteraceae family. The SEEDS yield oil and are used as food and animal feed; the roots of Helianthus tuberosus (Jerusalem artichoke) are edible.
The region of the stem beneath the stalks of the seed leaves (cotyledons) and directly above the young root of the embryo plant. It grows rapidly in seedlings showing epigeal germination and lifts the cotyledons above the soil surface. In this region (the transition zone) the arrangement of vascular bundles in the root changes to that of the stem. (From Concise Dictionary of Biology, 1990)
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.
A plant genus in the family FABACEAE which is the source of edible beans and the lectin PHYTOHEMAGGLUTININS.
A genus of PLANT VIRUSES, in the family CAULIMOVIRIDAE, that are transmitted by APHIDS in a semipersistent manner. Aphid-borne transmission of some caulimoviruses requires certain virus-coded proteins termed transmission factors.
A plant species of the family BRASSICACEAE best known for the edible roots.
A plant species of the family FABACEAE used to study GENETICS because it is DIPLOID, self fertile, has a small genome, and short generation time.
The relationship between an invertebrate and another organism (the host), one of which lives at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.
The interactions between a host and a pathogen, usually resulting in disease.
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
A mitosporic Leotiales fungal genus of plant pathogens. It has teleomorphs in the genus Botryotina.
Higher plants that live primarily in terrestrial habitats, although some are secondarily aquatic. Most obtain their energy from PHOTOSYNTHESIS. They comprise the vascular and non-vascular plants.
Any of the various plants of the genus Lactuca, especially L. sativa, cultivated for its edible leaves. (From American Heritage Dictionary, 2d ed)
Eukaryotes in the group STRAMENOPILES, formerly considered FUNGI, whose exact taxonomic level is unsettled. Many consider Oomycetes (Oomycota) a phylum in the kingdom Stramenopila, or alternatively, as Pseudofungi in the phylum Heterokonta of the kingdom Chromista. They are morphologically similar to fungi but have no close phylogenetic relationship to them. Oomycetes are found in both fresh and salt water as well as in terrestrial environments. (Alexopoulos et al., Introductory Mycology, 4th ed, pp683-4). They produce flagellated, actively motile spores (zoospores) that are pathogenic to many crop plants and FISHES.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A family of sterols commonly found in plants and plant oils. Alpha-, beta-, and gamma-isomers have been characterized.
Adaptation to a new environment or to a change in the old.
A plant species of the family APIACEAE that is widely cultivated for the edible yellow-orange root. The plant has finely divided leaves and flat clusters of small white flowers.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The spurge family of flowering plants, in the order Euphorbiales, contains some 7,500 species in 275 genera. The family consists of annual and perennial herbs and woody shrubs or trees.
The science, art or practice of cultivating soil, producing crops, and raising livestock.
Non-native organisms brought into a region, habitat, or ECOSYSTEM by human activity.
The total process by which organisms produce offspring. (Stedman, 25th ed)
The gourd plant family of the order Violales, subclass Dilleniidae, class Magnoliopsida. It is sometimes placed in its own order, Cucurbitales. 'Melon' generally refers to CUCUMIS; CITRULLUS; or MOMORDICA.
Genotypic differences observed among individuals in a population.
Plant proteins that mediate LIGHT SIGNAL TRANSDUCTION. They are involved in PHOTOTROPISM and other light adaption responses during plant growth and development . They include the phototropins, phytochromes (PHYTOCHROME), and members of the ubiquitous cryptochrome family.
A plant genus of the family LAMIACEAE best known for the thyme spice added to foods.
A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA.
A group of FLAVONOIDS derived from FLAVONOLS, which lack the ketone oxygen at the 4-position. They are glycosylated versions of cyanidin, pelargonidin or delphinidin. The conjugated bonds result in blue, red, and purple colors in flowers of plants.
A plant genus of the family POACEAE. The grain is used for FOOD and for ANIMAL FEED. This should not be confused with KAFFIR LIME or with KEFIR milk product.
Pesticides used to destroy unwanted vegetation, especially various types of weeds, grasses (POACEAE), and woody plants. Some plants develop HERBICIDE RESISTANCE.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
Proteins found in any species of bacterium.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
A plant family of the order Arales, subclass Arecidae, class Liliopsida (monocot). Many members contain OXALIC ACID and calcium oxalate (OXALATES).
The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.
A plant genus of the family ROSACEAE known for the edible fruit.
A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.
Substituted thioglucosides. They are found in rapeseed (Brassica campestris) products and related cruciferae. They are metabolized to a variety of toxic products which are most likely the cause of hepatocytic necrosis in animals and humans.
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.
The process of germ cell development in plants, from the primordial PLANT GERM CELLS to the mature haploid PLANT GAMETES.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Flammable, amorphous, vegetable products of secretion or disintegration, usually formed in special cavities of plants. They are generally insoluble in water and soluble in alcohol, carbon tetrachloride, ether, or volatile oils. They are fusible and have a conchoidal fracture. They are the oxidation or polymerization products of the terpenes, and are mixtures of aromatic acids and esters. Most are soft and sticky, but harden after exposure to cold. (From Grant & Hackh's Chemical Dictionary, 5th ed & Dorland, 28th ed)
A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.
Degree of saltiness, which is largely the OSMOLAR CONCENTRATION of SODIUM CHLORIDE plus any other SALTS present. It is an ecological factor of considerable importance, influencing the types of organisms that live in an ENVIRONMENT.
The absence of light.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
A ubiquitous sodium salt that is commonly used to season food.
High molecular weight polysaccharides present in the cell walls of all plants. Pectins cement cell walls together. They are used as emulsifiers and stabilizers in the food industry. They have been tried for a variety of therapeutic uses including as antidiarrheals, where they are now generally considered ineffective, and in the treatment of hypercholesterolemia.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A plant species of the genus VICIA, family FABACEAE. The edible beans are well known but they cause FAVISM in some individuals with GLUCOSEPHOSPHATE DEHYDROGENASE DEFICIENCY. This plant contains vicine, convicine, Vicia lectins, unknown seed protein, AAP2 transport protein, and Vicia faba DNA-binding protein 1.
Large and highly vacuolated cells possessing many chloroplasts occuring in the interior cross-section of leaves, juxtaposed between the epidermal layers.
The external elements and conditions which surround, influence, and affect the life and development of an organism or population.
A blue-green biliprotein widely distributed in the plant kingdom.
A plant family of the order Selaginellales, class Lycopodiopsida, division Lycopodiophyta, subkingdom Tracheobionta. Members contain bilobetin. The rarely used common name of resurrection plant is mainly used with CRATEROSTIGMA.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A monocot family within the order Liliales. This family is divided by some botanists into other families such as Convallariaceae, Hyacinthaceae and Amaryllidaceae. Amaryllidaceae, which have inferior ovaries, includes CRINUM; GALANTHUS; LYCORIS; and NARCISSUS and are known for AMARYLLIDACEAE ALKALOIDS.
Substances or mixtures that are added to the soil to supply nutrients or to make available nutrients already present in the soil, in order to increase plant growth and productivity.
The ability of organisms to sense and adapt to high concentrations of salt in their growth environment.
Partial cDNA (DNA, COMPLEMENTARY) sequences that are unique to the cDNAs from which they were derived.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
A plant genus of the family RUTACEAE. They bear the familiar citrus fruits including oranges, grapefruit, lemons, and limes. There are many hybrids which makes the nomenclature confusing.
A cluster of FLOWERS (as opposed to a solitary flower) arranged on a main stem of a plant.
Substances found in PLANTS that have antigenic activity.

A single limit dextrinase gene is expressed both in the developing endosperm and in germinated grains of barley. (1/4690)

The single gene encoding limit dextrinase (pullulan 6-glucanohydrolase; EC 3.2.1.41) in barley (Hordeum vulgare) has 26 introns that range in size from 93 to 822 base pairs. The mature polypeptide encoded by the gene has 884 amino acid residues and a calculated molecular mass of 97,417 D. Limit dextrinase mRNA is abundant in gibberellic acid-treated aleurone layers and in germinated grain. Gibberellic acid response elements were found in the promoter region of the gene. These observations suggest that the enzyme participates in starch hydrolysis during endosperm mobilization in germinated grain. The mRNA encoding the enzyme is present at lower levels in the developing endosperm of immature grain, a location consistent with a role for limit dextrinase in starch synthesis. Enzyme activity was also detected in developing grain. The limit dextrinase has a presequence typical of transit peptides that target nascent polypeptides to amyloplasts, but this would not be expected to direct secretion of the mature enzyme from aleurone cells in germinated grain. It remains to be discovered how the enzyme is released from the aleurone and whether another enzyme, possibly of the isoamylase group, might be equally important for starch hydrolysis in germinated grain.  (+info)

Characterization of Chlamydomonas reinhardtii zygote-specific cDNAs that encode novel proteins containing ankyrin repeats and WW domains. (2/4690)

Genes that are expressed only in the young zygote are considered to be of great importance in the development of an isogamous green alga, Chlamydomonas reinhardtii. Clones representing the Zys3 gene were isolated from a cDNA library prepared using zygotes at 10 min after fertilization. Sequencing of Zys3 cDNA clones resulted in the isolation of two related molecular species. One of them encoded a protein that contained two kinds of protein-to-protein interaction motifs known as ankyrin repeats and WW domains. The other clone lacked the ankyrin repeats but was otherwise identical. These mRNA species began to accumulate simultaneously in cells beginning 10 min after fertilization, and reached maximum levels at about 4 h, after which time levels decreased markedly. Genomic DNA gel-blot analysis indicated that Zys3 was a single-copy gene. The Zys3 proteins exhibited parallel expression to the Zys3 mRNAs at first, appearing 2 h after mating, and reached maximum levels at more than 6 h, but persisted to at least 1 d. Immunocytochemical analysis revealed their localization in the endoplasmic reticulum, which suggests a role in the morphological changes of the endoplasmic reticulum or in the synthesis and transport of proteins to the Golgi apparatus or related vesicles.  (+info)

GTPase activity and biochemical characterization of a recombinant cotton fiber annexin. (3/4690)

A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta-glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation.  (+info)

A multisubunit acetyl coenzyme A carboxylase from soybean. (4/4690)

A multisubunit form of acetyl coenzyme A (CoA) carboxylase (ACCase) from soybean (Glycine max) was characterized. The enzyme catalyzes the formation of malonyl CoA from acetyl CoA, a rate-limiting step in fatty acid biosynthesis. The four known components that constitute plastid ACCase are biotin carboxylase (BC), biotin carboxyl carrier protein (BCCP), and the alpha- and beta-subunits of carboxyltransferase (alpha- and beta-CT). At least three different cDNAs were isolated from germinating soybean seeds that encode BC, two that encode BCCP, and four that encode alpha-CT. Whereas BC, BCCP, and alpha-CT are products of nuclear genes, the DNA that encodes soybean beta-CT is located in chloroplasts. Translation products from cDNAs for BC, BCCP, and alpha-CT were imported into isolated pea (Pisum sativum) chloroplasts and became integrated into ACCase. Edman microsequence analysis of the subunits after import permitted the identification of the amino-terminal sequence of the mature protein after removal of the transit sequences. Antibodies specific for each of the chloroplast ACCase subunits were generated against products from the cDNAs expressed in bacteria. The antibodies permitted components of ACCase to be followed during fractionation of the chloroplast stroma. Even in the presence of 0.5 M KCl, a complex that contained BC plus BCCP emerged from Sephacryl 400 with an apparent molecular mass greater than about 800 kD. A second complex, which contained alpha- and beta-CT, was also recovered from the column, and it had an apparent molecular mass of greater than about 600 kD. By mixing the two complexes together at appropriate ratios, ACCase enzymatic activity was restored. Even higher ACCase activities were recovered by mixing complexes from pea and soybean. The results demonstrate that the active form of ACCase can be reassembled and that it could form a high-molecular-mass complex.  (+info)

Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation. (5/4690)

We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398 amino acid residues and is predicted to have six membrane-spanning domains. To obtain evidence for the postulated function of the Arabidopsis gene, transgenic plants with the ZAT coding sequence under control of the cauliflower mosaic virus 35S promoter were analyzed. Plants obtained with ZAT in the sense orientation exhibited enhanced Zn resistance and strongly increased Zn content in the roots under high Zn exposure. Antisense mRNA-producing plants were viable, with a wild-type level of Zn resistance and content, like plants expressing a truncated coding sequence lacking the C-terminal cytoplasmic domain of the protein. The availability of ZAT can lead to a better understanding of the mechanism of Zn homeostasis and resistance in plants.  (+info)

The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants. (6/4690)

The Dof proteins are a large family of plant transcription factors that share a single highly conserved zinc finger. The tobacco Dof protein NtBBF1 was identified by its ability to bind to regulatory domain B in the promoter of the rolB oncogene. In this study, we show that the ACT T TA target sequence of NtBBF1 in domain B is necessary for tissue-specific expression of rolB. beta-Glucuronidase (GUS) activity of tobacco plants containing a rolB promoter-GUS fusion with a mutated NtBBF1 target sequence within domain B is almost completely suppressed in apical meristems and is severely abated in the vascular system. The ACT T TA motif is shown here also to be one of the cis-regulatory elements involved in auxin induction of rolB. The pattern of NtBBF1 expression in plants is remarkably similar to that of rolB, except in mesophyll cells of mature leaves, in which only NtBBF1 expression could be detected. Ectopic expression of rolB in mesophyll cells was achieved by particle gun delivery if the NtBBF1 binding sequence was intact. These data provide evidence that in the plant, a Dof protein DNA binding sequence acts as a transcriptional regulatory motif, and they point to NtBBF1 as the protein involved in mediating tissue-specific and auxin-inducible expression of rolB.  (+info)

Isolation and characterization of SYN1, a RAD21-like gene essential for meiosis in Arabidopsis. (7/4690)

The proper pairing, recombination, and segregation of chromosomes are central to meiosis and sexual reproduction. The syn1 mutation was previously identified as a synaptic mutant in a T-DNA-tagged population of plants. SYN1 has been isolated and found to exhibit similarity to Schizosaccharomyces pombe RAD21 and RAD21-like proteins, which are required for chromosome condensation and sister chromatid cohesion during mitosis. Plants homozygous for syn1 are male and female sterile and show defects in chromosome condensation and pairing beginning at leptonema of meiosis I. Fragmentation of the chromosomes was observed at metaphase I. Alternative promoters produced two SYN1 transcripts. One transcript was expressed at low levels in most tissues, whereas the other was expressed only in prebolting buds. DNA blot analyses suggest that Arabidopsis contains a small RAD21 gene family. Consistent with the DNA blot data, a second Arabidopsis RAD21-like gene has been identified. These results suggest that different RAD21-like proteins play essential roles in chromosome condensation and pairing during both meiosis and mitosis.  (+info)

The FLF MADS box gene: a repressor of flowering in Arabidopsis regulated by vernalization and methylation. (8/4690)

A MADS box gene, FLF (for FLOWERING LOCUS F ), isolated from a late-flowering, T-DNA-tagged Arabidopsis mutant, is a semidominant gene encoding a repressor of flowering. The FLF gene appears to integrate the vernalization-dependent and autonomous flowering pathways because its expression is regulated by genes in both pathways. The level of FLF mRNA is downregulated by vernalization and by a decrease in genomic DNA methylation, which is consistent with our previous suggestion that vernalization acts to induce flowering through changes in gene activity that are mediated through a reduction in DNA methylation. The flf-1 mutant requires a greater than normal amount of an exogenous gibberellin (GA3) to decrease flowering time compared with the wild type or with vernalization-responsive late-flowering mutants, suggesting that the FLF gene product may block the promotion of flowering by GAs. FLF maps to a region on chromosome 5 near the FLOWERING LOCUS C gene, which is a semidominant repressor of flowering in late-flowering ecotypes of Arabidopsis.  (+info)

There is a large literature on gene silencing, in which the transgenes remain in the genome, but are not expressed. More serious, from the safety point of view, is structural instability, the tendency for the transgenic DNA to come loose, to rearrange or become lost in part or in whole in successive generations [2,3]. This could change the transgenic line in unpredictable ways in terms of health and environmental risks. And it will increase the chance of transgenic DNA being taken up by unrelated species to make new combinations with their genetic material. Thats referred to as horizontal gene transfer and recombination. Transgenic DNA can spread to every species that interact with the transgenic plant, in the soil, in the air, in the mouth and gut and the respiratory tracts of animals including human beings ...
The Mag-Bind® Plant DNA DS 96 Kit allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content. The lysis and binding buffers are specifically designed to minimize co-purification of polysaccharides and polyphenols. Up 96 samples of 50 mg wet tissue (or 15 mg dry tissue) can be processed in parallel in less than one hour. The system combines CTAB-based lysis, which eliminates the need for organic solvents, with the convenience of Mag-Bind® Particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for PCR, restriction digestion, next generation sequencing, and hybridization applications. There are no organic extractions thereby reducing consumables and decreasing hands-on time to allow ...
Request for plant DNA methods from diverse species and different structures. Purpose of Project Green Gene is to isolate and preserve DNA and cDNA libraries from diverse plant species. These libraries are to then be supplied to researchers and the DNA preserved for thousands of years. Please support the people of PROJECT GREEN GENE with your ideas and helpful methods. Sincerely, Richard Dana, Ph.D. Project Green Gene Ambiocom Box 910416 San Diego, CA 92191-0416 Call the bbs: 619-945-2321 jobs/resumes on-line Thank you to the people who have supported this project and offered methods. DNA methods, RNAID methods, first stand systhesis methods. Please help ...
The Maxwell 16 LEV Plant DNA Kit is used with the Maxwell 16 Research Instrument (Cat.# AS2000) to provide an easy method for efficient, automated purification of genomic DNA (gDNA) from plant tissue samples.
Gentaur molecular products has all kinds of products like :search , Biolin \ ISOLATE Plant DNA Mini Kit \ BIO-52035 for more molecular products just contact us
The Presto™ 96 Well Plant Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from various plant species. Homogenized samples are treated with RNase A then centrifuged to remove cell debris and salt precipitates.
estudiantbiochem,. As long as you handle the plant tissue properly, there should be no difference between your fresh and dried samples. This means that you should either freeze your tissue at -70C to -80C or lyophilize your tissue immediately after you harvest it. This will minimize DNase activity in the plant tissue before you extract the DNA.. AronD. ...
Most of the cells in our body have very low levels of telomerase, and thus age as they divide (picture the shoelace tips wearing away until they are gone). Aging cells equal an aging body, with cells no longer functioning normally.
To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...
The discovery in Mexico of native corn varieties contaminated by transgenic DNA should raise alarm bells for New Zealand. Tested under government sponsored research, 15 of 22 sites proved positive. Researchers identified DNA sequences from the cauliflower ...
Competition between specific and non-specific amplification at extremely low level DNA amounts.With extremely low-level DNA amounts (|∼2 copies), stochasti
An experiment was conducted to investigate the effect of phytase transgenic corn (PTC) on intestinal microflora, and the fate of transgenic DNA and protein in the digesta and tissues of broilers. A total of 160 1-day-old Arbor Acres commercial male broilers were randomly assigned to 20 cages (8 chicks per cage) with 10 cages (replicates) for each treatment. Birds were fed with a diet containing either PTC (54.0% during 1-21 days and 61.0% during 22-42 days) or non-transgenic isogenic control corn (CC) for a duration of 42 days. There were no significant differences (P|0.05) between birds fed with the PTC diets and those fed with the CC diets in the quantities of aerobic bacteria, anaerobic bacteria, colibacillus and lactobacilli, or microbial diversities in the contents of ileum and cecum. Transgenic phyA2 DNA was not detected, but phyA2 protein was detected in the digesta of duodenum and jejunum of broilers fed with the PTC diets. Both transgenic phyA2 DNA and protein fragments were not found in the
smitty777 writes Highlighting another unique way to use cutting edge DNA technology, the U.S. Department of Defense has a new weapon in its efforts to combat counterfeit parts: plant DNA. This article at Wired discusses how plant DNA can be used to make an almost unique code (1 in 1 trillion) for ...
The significance of the 4C value (where C is the amount of DNA in the unreplicated haploid genome) in angiosperm plants is discussed. The DNA amount is a stable feature used in biosystematics. Although this parameter varies even in closely related taxa, there is no correlation between the DNA amount and the structural and functional organization of plants. The role of DNA amount, including excess DNA, in plant evolution is considered. Some rules governing the distribution of DNA amount among different plant taxa are postulated, together with the possibility of using the data in systematics, phylogeny, and solutions of problems of genetic apparatus organization and evolution. The decrease in DNA value per genome during plant evolution and the high level of species formation in taxa with large DNA values have been shown. Plant taxa with a small DNA value per genome have a high percentage and higher degree of polyploidy. The nature of the differential staining of euchromatin and heterochromatin bands of
99 , 5634 - 5639 . 10.1016/j.biortech.2007.10.036 Muthukumar , A. , Sangeetha , G. & Naveenkumar , R. ( 2016 ): Antimicrobial activity of essential oils against seed borne fungi of rice ( Oryza sativa L.) . J. Environ. Biol. , 37 , 1429. ...
Lucigen strives to provide life scientists with the highest quality products and services for RNA/DNA amplification, cloning, next gen sequencing, and protein expression. Experience outstanding performance with time-saving convenience at an exceptional price.
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Stony Brook-based Applied DNA Sciences, a biotech firm that uses plant DNA to make anti-counterfeiting products, has signed a two-year deal with a Melville startup to sell a line of document authentic
View Notes - ga1 from CS 723 at Jaypee University IT. Geneticalgorithms Introduction [email protected] GeneticAlgorithmsinaslide Premise Evolutionworkedonce(itproducedus!),itmightworkagain
Our knowledge of natural genetic variation is increasing at an extremely rapid pace, affording an opportunity to come to a much richer understanding of how effects of specific genes are dependent on the genetic background. To achieve a systematic understanding of such GxG interactions, it is desirable to develop genome editing tools that can be rapidly deployed across many different genetic varieties. We present an efficient CRISPR/Cas9 toolbox of super module (SM) vectors. These vectors are based on a previously described fluorescence protein marker expressed in seeds allowing identification of transgene-free mutants. We have used this vector series to delete genomic regions ranging from 1.7 to 13 kb in different natural accessions of the wild plant Arabidopsis thaliana. Based on results from 53 pairs of sgRNAs targeting individual nucleotide binding site leucine-rich repeat (NLR) genes, we provide a comprehensive overview of obtaining heritable deletions. The SM series of CRISPR/Cas9 vectors enables
If we have dry and scaly skin problems, watermelon fruit is one of the good fruit to help solve dry and scaly skin problems. Watermelon water contains a very abundant moisture content that can provide extra moisture to our skin. Consume watermelon fruit also keep the body to avoid dehydration. To maximize the fruit watermelon for beauty and health of our skin, in addition to the consumption directly, you can also use this watermelon fruit as a natural mask to provide a maximum result for the moisture of our skin. Use a fruit mask watermelon once a week 2times to get the result we want ...
Combining plant DNA is possible by grafting or cross-pollinating two different species of plant to create a new species. Understand how DNA is combined to produce hybrid fruits and vegetables with information from a biology teacher in this free video on science. Expert: Janice Crenetti Contact: WeAreHDTV.com Bio: Janice Creneti has a Bachelor of Science in secondary science education and a Bachelor of Art in biology from Boston University. Filmmaker: Christopher Rokosz
A set of 13 simple sequence repeat markers was developed from D. trimaculatus genomic DNA, tested for D. auripinnis and characterized using 40 individuals per species. All the loci were polymorphic with a number of alleles ranging from three to 30. Observed heterozygosities varied from 0.23 to 0.89 for D. trimaculatus and from 0.11 to 0.85 for D. auripinnis. Early results show that these will be powerful markers for the study of ecological and evolutionary mechanism in this coral reef fish species complex ...
Diversity in plant genomes remains largely unexplored. The 10,000 Plant Genome Sequencing Project is a landmark effort to catalogue plant genomic variation, representing a major step in understanding the tree of life. The project offers new opportunities to study biological processes and address fundamental research questions.
What is the difference between Autopolyploidy and Allopolyploidy? Autopolyploidy and Allopolyploidy are two main types of polyploidy. Autopolyploidy is the...
i am attempting an experiment to cut time and expense in processing large numbers of small-diameter plant stems for subsequent PCR detection of MLOs. i cannot find the original biotechniques article i intended to use, which described a brief grinding of tissue, quick boil prep, and immediate PCR of dilutions taken directly from the boiling prep. can someone direct me to this or a similar protocol? thanks. john rascoe department of entomology and plant pathology 110 noble research center, OSU stillwater, ok 74078 ...
DNA barcoding is a way to identify species via their species-specific genetic signatures. To do this for pollen, scientists sequence the DNA from a genetic region known to occur in all plants, but which varies from species to species. There are two parts to the standardized sequence we use for plant DNA barcoding. One is a section of the large subunit of a gene called ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL for short). The other is a gene called maturase-K (matK). These genes are both essential for a plant to survive, and are thus present in all plants. Once an investigator sequences these gene regions from a sample, they can be compared to a database containing all the known DNA sequences of rbcL and matK to identify the species ...
The function of the nucleus in the plant cell is to store the plants DNA and control the activity of the cell through protein synthesis. The nucleus is en
XcelGen plant DNA mini kit is designed for the rapid purification of genomic DNA from a variety of fresh & dry plant materials like leaves tissues, roots. The plant samples are homogenized under high chaotropic buffer followed by removal of contamination. The resultant lysate is transfer to silica membrane, which help in selective binding of DNA in presence of high salt. Following binding of DNA to the silica membrane, contaminants are washed away and pure genomic DNA is eluted ...
Our kits for preamplification PCR or preamplification qPCR amplify cDNA and genomic DNA from samples containing low RNA and DNA amounts
The selection committee (composed of the present, past, and incoming chairs of FRBR) will decide whether the nominated cultivar or germplasm meets the criteria established for the award. The recipient may be a breeder, releaser, or institution responsible for the cultivar or germplasm release; the recipient need not be an ASHS Member ...
Read The rice R gene family: two distinct subfamilies containing several miniature inverted-repeat transposable elements, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Review: Ancient plant DNA in lake sediments on Plantae | Fossils have been extremely useful in efforts to reconstruct the past, but recently the analysis of…
Principal coordinate analysis (PCoA) plot of samples from ground and flight mice.Flight mice data points clustered relatively tightly within the bottom right qu
摘要】:[Background] Eating and cooking qualities(ECQs) of rice(Oryza sativa L.) determine consumer acceptance and the economic value of rice varieties. Genetic factors are required for development of rice varieties with excellent ECQs and association mapping is one of the promising approaches for discovering such associated genetic factors. [Material and method] A genome-wide association mapping was performed on a set of 253 non-glutinous rice accessions consisting of 83 indica and 170 japonica Asian cultivated rice varieties through phenotyping for 11 ECQ traits in two consecutive years and genotyping with 210 polymorphic SSR and candidate-gene markers. [Results] These markers amplified 747 alleles with an average of 3.57 alleles per locus. The structure, phylogenetic relationship, and principal component analysis indicated a strong population differentiation between indica and japonica accessions and association mapping was thus undertaken within indica and japonica subpopulations. All ...
OsSLI1, a Homeodomain Containing Transcription Activator, Involves Abscisic Acid Related Stress Response in Rice Oryza sativa L.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
PlantZol,Genomic DNA Purification,Nucleic Acid Purification,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionPlantZol provides an easy and fast method to isolate hi
WATERMeLON Fruit Stand JackPOT ! Every village we go to has huge juicy watermelon! Plus a line of garlic ~the sweet fruit stand lady really wanted us to take a
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Dormant viruses can be reactivated with genetically modified organisms - new research see also http://www.scup.no/mehd/ho New Research Results on Genetically Modified Organisms The use of the Cauliflower Mosaic Viral promotor (CaMV) has the potential to reactivate dormant viruses or create new viruses in all species to which it is transferred. CaMV is known to be found in practically all current transgenic crops released commercially or undergoing field trials. This transgenic instability increases the possibility of promotion of an inappropriate over-expression of genes to the transferred species. The development of cancer may be one consequence of such inappropriate over-expression of genes. The scientists behind the research strongly recommend that all transgenic crops containing CaMV 35S or similar promoters which are recombinogenic should be immediately withdrawn from commercial production or open field trials. All products derived from such crops containing transgenic DNA should also be ...
Rice is a cereal plant and staple food for most Indonesian people. One of the causes of the problem is decrease of wetland and lowland area which have impact on national level production. One of the efforts to increase rice production is selection to get desired trait. Regression and correlation analysis to determined the relationship among characters that can be used as a consideration for selection criterion. The purpose of this study is to know the relationships between morphological and agronomic characters in rice plant of F2 generation. This study was conducted at the Experimental Faculty of Agriculture, University of Brawijaya, Jatimulyo Village, Malang, between June and August 2018. Planting materials were population of F2 generation (SBCH, SBCB, TWCH dan TWCB). All of the populations planted in area 25 m x 3 m with spacing 60 cm x 60 cm. Based on the result, all of the populations showed that there was positif linear relationship between plant height and panicle length, number of ...
Arias, S. & al. (2005) Phylogenetic relationships in Peniocereus (Cactaceae) inferred from plastid DNA sequence data. Journal of Plant Research 118 (5): 317-328 ...
We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true ...
A partial gallery of selects from the 2015 SSS 3 Bridge Fiasco...If you would like to see additional frames please contact! www.pressure-drop for full report and to add your own insights!
This file defines the functional laboratory areas. Each area may have multiple accession areas. Entries may be added, but supplied entries should not be modified or deleted. It is pointed to by the COLLECTION SAMPLE and ACCESSION TEST GROUP files ...
Cultivated peanut is an allotetraploid with two nuclear genomic components, AA and BB. Although it is generally agreed that these component genomes are derived from diploid wild ancestors, the exact species involved has been a matter of some research and discussion. Although the evidence is not completely clear cut, analysis of data from molecular markers, cytogenetics, morphology and geographical distributions support that A. duranensis and A. ipaënsis are the direct ancestors of cultivated peanut [8, 30].. Genomic in situ hybridization (GISH) of A. hypogaea metaphase chromosomes with total genomic DNA from the AA genome of A. duranensis and the BB genome of A. ipaënsis allowed a clear differentiation of the A and B chromosomes. Firstly, this observation reinforces the evidence of the close relationship between the genomes of A. duranensis, A. ipaënsis and cultivated peanut. Secondly, since GISH relies largely on the hybridization of repetitive sequences, it also indicates that A. duranensis ...
The goals of this investigation were to identify and evaluate the use of polymorphic microsatellite marker (PMM) analysis for molecular typing of seventeen plant pathogenic fungi. Primers for di-, tri-, and tetranucleotide loci were designed directly from the recently published genomic sequence of Mycospherlla graminicola and Fusarium graminearum. A total of 20 new microsatellite primers as easy-to-score markers were developed. Microsatellite primer PCR (MP-PCR) yielded highly reproducible and complex genomic fingerprints, with several bands ranging in size from 200 to 3000 bp. Of the 20 primers tested, only (TAGG)4, (TCC)5 and (CA)7T produced a high number of polymorphic bands from either F. graminearum or F. culmorum. (ATG)5 led to successful amplifications in M. graminicola isolates collected from Germany. Percentage of polymorphic bands among Fusarium species ranged from 9 to 100%. Cluster analysis of banding patterns of the isolates corresponded well to the established species delineations based
and thus are genetically linked. The primers used to generate polymorphic bands were 3-anchored inter-simple sequence repeat primers which identified genomic microsatellites with a repeated motif of 3 nucleotides in length. The primers were used singly to amplify genomic segments which were flanked by inversely orientated, closely spaced, identical microsatellite sequences. One of the polymorphic bands, a 900 base pair band, was completely linked to the Sr39 and Lr35 rust resistance genes in the segregating population used in this study. After cloning and sequencing this polymorphic band, the inter-simple sequence repeat marker was converted to a sequence characterized amplified region marker by designing primer sets which amplify a single, easily resolved band from DNA of plants with Sr39/Lr35 genes. This marker is present in six wheat lines carrying the Sr39 and Lr35 genes on the translocated chromosome segment from Ae. speltoides, The marker has facilitated efforts to breed Canada Prairie ...
MagListoTM 5M Plant Genomic DNA Extraction Kit 는 magnetic nano bead와 MagListoTM를 이용하여 Plant sample (leaf, root, seed) 에서 Genomic DNA를 빠르게 추출할 수 있는 획기적인 제품입니다. Magnetic nano bead와 자석을 이용해 세포 분쇄물 중 Genomic DNA만을 분리시키고 농축 및 정제하는 과정을 거치기 때문에 원심분리기를 사용하는 방법에 비해 빠르게 DNA를 분리 할 수 있습니다. 본 제품은 mini, midi, maxi scale의 prep을 위해 별도의 kit를 구매하지 않고 한 가지의 kit를 이용해 모두 prep 할 수 있으며 midi나 maxi prep을 위해 별도의 vacuum system이나 air pressure system을 구비 할 필요가 없는 것이 장점입니다.
Arachis villosulicarpa is a perennial peanut species, which is cultivated by indigenous people in Mato Grosso, a state of Brazil. Its wild progenitor is thought to be Arachis pietrarellii. Although it is related to the common peanut, Arachis hypogaea, it was separately domesticated: A. villosulicarpa is diploid, whereas A. hypogaea is tetraploid. It is one of several species that might be used as gene source for plant breeding to improve the important cultivated peanut Arachis hypogaea. List of domesticated plants Galgaro, L.; Montenegro Valls, J. F.; Lopes, C. R. (1997). Study of the genetic variability and similarity among and within Arachis villosulicarpa, A. pietrarellii and A. hypogaea through isoenzyme analysis. Genetic Resources and Crop Evolution. 44: 9. doi:10.1023/A:1008609115357. Wynne, J. C.; Beute, M. K.; Nigam, S. N. (1991). Breeding for Disease Resistance in Peanut (Arachis Hypogaea L.). Annual Review of Phytopathology. 29: 279. doi:10.1146/annurev.py.29.090191.001431. ...
An allopolyploid is an individual having two or more complete sets of chromosomes derived from different species. Generation of allopolyploids might be rare because of the need to overcome limitations such as co-existing populations of parental lines, overcoming hybrid incompatibility, gametic non-reduction, and the requirement for chromosome doubling. However, allopolyploids are widely observed among plant species, so allopolyploids have succeeded in overcoming these limitations and may have a selective advantage. As techniques for making allopolyploids are developed, we can compare transcription, genome organization, and epigenetic modifications between synthesized allopolyploids and their direct parental lines or between several generations of allopolyploids. It has been suggested that divergence of transcription caused either genetically or epigenetically, which can contribute to plant phenotype, is important for the adaptation of allopolyploids.
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
Rocky Mountain Herbarium specimen database search page providing access to nearly 700,000 specimen records, primarily from the Rocky Mountain Region of North America.
Mori, N.; Liu, Y.G.; Nakamura, C.; Tsunewaki, K., 1991: Genetic differentiation between two wild tetraploid wheats, Triticum dicoccoides and T. araraticum as revealed by RFLP analysis of organellar and nuclear DNA
This book is provided with plasmid DNA of 1,069 RAFL clones that correspond to Arabidopsis transcription factors. (The DNA samples are spotted on the pages of the book ...
Chromosomes associate premeiotically and in xylem vessel cells via their telomeres and centromeres in diploid rice (Oryza sativa). ...
Watermelon fruit slices from Nuts.com are classic fruit slices with the sweet flavors of summer and watermelon. Great prices and ready to ship.
Transgenic technologies used for creating a desired genomic change in animals involve three critical steps: isolation of fertilized eggs, microinjection of transgenic DNA into them and their subsequent transfer to recipient females
Oryza sativa, commonly known as Asian rice, is the plant species most commonly referred to in English as rice. Oryza sativa is a grass with a genome consisting of 430Mb across 12 chromosomes. It is renowned for being easy to genetically modify, and is a model organism for cereal biology.
水稻是單子葉植物,有亞洲栽培稻Oryza sativa(Asian rice)和非洲栽培稻Oryza glaberrima(African rice)兩種。作為穀物,在大部分的世界人口中,尤其是亞洲,屬最重要的糧食作物之ㄧ。根據2009 年的數據,在全球生產糧食的產量中,稻米產量排世界第三大,僅次於玉米和小麥。本研究發現經食源性誘導之C57BL / 6 mice 呈現高血糖、高尿糖、體重上升等非胰島素依賴型糖尿病症狀;此外,二至八組小鼠皆有頻渴、多尿、貪食等症狀。抗氧化實驗中,DPPH 自由基清除的效力以TNG 78 水萃取物清除率最佳,紫米水萃取物次之。餐後血糖變化值實驗中,TNG 78 血糖上升幅度較小,下降幅度較快。糙米之水萃取物對α-葡萄糖苷酶具抑制作用,在100 mg/ mL 之濃度時,TK 9, TNG 76, Purple Rice 水萃取物皆具五成以上的抑制率,Purple Rice 水萃取物的抑制率甚至高達九成
My research interests focus on the regulation of plant gene expression in response to abiotic stress and extreme environments, with particular interest in chromatin structure, genome organization and epigenetic change. Venues associated with spaceflight provide an opportunity to explore plant genomic responses to an environment that is outside the evolutionary experience of terrestrial organisms. This unique platform presents a background by which adaptive metabolisms can be observed as they are crafted to cope with a stress de novo; providing a window into the origins of adaptive processes.. ...
Hassler, M. 2020. Orobanchaceae. World Plants: Synonymic Checklists of the Vascular Plants of the World (2019). In: Roskov Y., Abucay L., Orrell T., Nicolson D., Bailly N., Kirk P., Bourgoin T., DeWalt R.E., Decock W., De Wever A., Nieukerken E. van, Zarucchi, J., Penev L., eds. 2020. Species 2000 & ITIS Catalogue of Life. Published on the internet. Accessed: 2020 Feb. 4 ...
In order to dissect the inheritance of the grapevine early ripening trait, Pearl of Csaba, 32 derived varieties of Pearl of Csaba and 5 other parents for the breeding of the derived varieties were analyzed based on the SSR (Simple Sequence Repeats) markers. Fifty SSR markers evenly distributed on 19 chromosomes of grapevine were initially employed and only 36 markers could transmit to all the desc ...
CCDB aims to combine existing data resources into an extensive central database that will be updated regularly by the community. Users and researchers are encouraged to contribute to the accuracy and completeness of the data in CCDB ...
Buy Plant Genome Analysis by Peter M. Gresshoff from Waterstones today! Click and Collect from your local Waterstones or get FREE UK delivery on orders over £20.
Nous avons trouvé que le contenu en ADN nucléaire varie peu chez les lamproies. Dans les 3 familles principales ce contenu représente en moyenne le 40% de celui de lhomme. Il existe une corrélation entre le contenu en ADN et le caryotype dans presque tous les genres.. ...
Sasaki H, Hara T, Ito S, Uehara N, Kim H, Lieffering M, Okada M, Kobayashi K,,2007,Effect of free-air CO2 enrichment on the storage of carbohydrate fixed at different stages in rice (Oryza sativa L.),Field Crops Research 100 (2431 ...
Native: indigenous.. Non-native: introduced (intentionally or unintentionally); has become naturalized.. County documented: documented to exist in the county by evidence (herbarium specimen, photograph). Also covers those considered historical (not seen in 20 years). State documented: documented to exist in the state, but not documented to a county within the state. Also covers those considered historical (not seen in 20 years).. Note: when native and non-native populations both exist in a county, only native status is shown on the map.. ...
Mer-Man 228 Sy-Klone 41 Stratos 162 Buzz-Off 52 Frosta 1 I know that its a little early, but I thought that there should be a thread were we post what we got as our Frosta Forearm Fiasco
Mer-Man 228 Sy-Klone 41 Stratos 162 Buzz-Off 52 Frosta 1 I know that its a little early, but I thought that there should be a thread were we post what we got as our Frosta Forearm Fiasco
Hosted by the USGS Core Science Analytics and Synthesis. Page designed through the cooperative efforts of interagency ITIS Teams. Point of Contact: [email protected]
A cactus ( Cactaceae spp. ) is similar to a house cat. Although it requires very little from you, it is not completely self-sufficient. A plant that is wilting or turning brown is begging for attention. You can revive the plant by finding and correcting the problem.
Another point to add to the list, is that the phase 2 PTA treated (non sham) people were technically not treated. The outcome percentage or flow created from the PTA was not to a standard that is defined as optimal ...
4 Plant sources. *5 Repair of psoralen DNA adducts. *6 Analysis of nucleic acids structures ... Repair of psoralen DNA adducts[edit]. PUVA treatment produces both DNA interstrand crosslinks (ICLs) and monoadducts. The ICLs ... defects in DNA repair increase gene copy number variation". DNA Repair (Amst.). 21: 87-96. doi:10.1016/j.dnarep.2014.05.011. ... Plant sources[edit]. Ficus carica (fig) is probably the most abundant source of psoralens. They are also found in small ...
... dna. "Mundra world's largest coal-fired pvt power plant". The Times of India. "Adani Power wants tariff revision for more power ... The power plant is one of the coal-based power plants of Adani Power. The coal for the power plant is imported primarily from ... The plant has nine power generating units, unit# 5 to 9 involves super-critical boiler technology. In July 2012 Adani Power ... Source of water for the power plant is sea water from the Gulf of Kutch. It is the world's 11th-largest single location coal- ...
DNA. 7 November 2009. "Prison to grow medicinal plants". The Times of India. 23 September 2007. Archived from the original on 3 ... In 2007, in an effort to promote Indian medicinal plants, 8,500 saplings of sandalwood were planted within the central jail ... "Yerawada jail - From Mahatma Gandhi to Ajmal Kasab". DNA. 21 November 2012. "Painter, cook or… Gawli gets to choose a job in ...
dna Web Team (5 December 2015). "Chennai floods: AIADMK denies allegations of relief materials being allowed only after ... Car makers were also hugely affected due to shut down of plants, thus leading to lower production volume. German automaker BMW ... Motorcycle producer Royal Enfield shut its Chennai offices on 1 December, as well as its plants in Thiruvotriyur and Oragadam, ... "With Chennai airport shut". DNA India. Chennai. 5 December 2015. "Airfares up as rail disruptions push people to fly". The ...
"2.81 cr saplings planted in a day; Forest Deptt makes it to Limca Book of Records". thehitavada.com. Retrieved 1 November 2017 ... "Years of drought sees high enrolment for Green Army in Latur , Latest News & Updates at Daily News & Analysis". dna. 15 March ... As part of this initiative, 2.81 crore saplings were planted on a single day, July 1, 2016 - earning the initiative a place in ... As the Minister of Forests, he initiated a massive state-wide plantation drive which aimed at planting 50 crore saplings by ...
DNA India. Retrieved 2020-12-24. "Gujarat's 214MW solar park named as Asia's largest single PV plant". PV Tech. 23 April 2012. ... Gujarat Solar Park-1 (also called Charanka Solar Park) is solar power plant near Charanka village in Patan district of Gujarat ... GACL (Gujarat Alkalies and Chemicals Limited) is setting up 30 MW Solar PV plant, and GNFC (Gujarat Narmada Valley Fertilizers ... Correspondent, dna (2018-04-21). "Solar power capacity at Charanka Solar Park to touch 790 MW". ...
Your mango is ripened using carbide". dna. 2013-05-18. Retrieved 2018-08-25. "Eating Artificially Ripened Fruits is Harmful". " ... Plant Physiol. 43 (8): 1255-1258. doi:10.1104/pp.43.8.1255. PMC 1087003. PMID 16656908.CS1 maint: multiple names: authors list ...
"Tata Coffee shares up after plans of new plant in Vietnam". dna. 20 December 2016. Retrieved 25 July 2018. "Tata Coffee ...
"Reliance to invest Rs 60,000-cr for Orissa power plant". dna. Archived from the original on 3 September 2014. Retrieved 31 ... Rourkela Steel Plant was the first integrated steel plant in the public sector in India, built with collaboration of Germany. ... Russian major Magnitogorsk Iron and Steel Company (MMK) plans to set up a 10 MT steel plant in Odisha, too. Bandhabahal is a ... It has 1078 species of plants, including 94 orchids. The sal tree is the primary tree species there. The park has 55 mammals, ...
... dna. Retrieved 20 April 2015. cctindia. "Uran Gas Turbine Power Station (Major India Gas Power Plants)". Retrieved 20 April ... The power plant is operated by the Maharashtra State Power Generation Company (Mahagenco). { getch( ) } It has an installed ... Uran Gas Turbine Power Station is a gas-based thermal power plant located at Uran in Raigad district, Maharashtra. ... "Major Thermal Plants". Retrieved 20 April 2015. v t e. ... "MahaGenco may put Uran plant expansion on hold". ...
... dna. 16 September 2014. Retrieved 6 February 2017. CS1 maint: discouraged parameter (link) "Uranium plant to come up in Kadapa ... A uranium purification plant is usually located closely to the mine as uranium ore is converted here to yellow cake. According ... The plant processes 2,190 tonnes uranium ore per day. After purification of the ore, yellow cake is shipped by heavy-duty ... of the mined ore is treated as waste as the uranium isotopes used in nuclear power plants mainly is uranium-235 leaving behind ...
"Maharashtra's largest thermal power plant to close all units by May 15". dna. Retrieved 20 April 2015. http://zeenews.india.com ... The power plant is one of the coal based power plants of MAHAGENCO. The coal for the power plant is sourced from Durgapur and ... With the total capacity of 3340MW, the plant is the largest power plant in the Maharashtra. It accounts for more than 25% of ... In the summer of 2010 due to less water in Erai, the plant also got water supply from Chargaon Dam. New 2 units of CSTPS India ...
"Mumbaikars save 4,000 plants on WEH from Metro axe". The Hindu. Retrieved 6 April 2017.. ... "Work on Metro-7 extension to start in six months: MMRDA". dna. 18 September 2018. Retrieved 9 October 2018. "Mumbai: Work on ... "Mumbai: Repair of MGL gas pipeline puncture to begin today". dna. 31 March 2017. Retrieved 4 April 2017. "Within 10 months, ... "Study of building height restrictions near Mumbai airport soon , Latest News & Updates at Daily News & Analysis". dna. 24 April ...
... which corresponds to RNAPs in plant chloroplasts. The beta subunits are responsible for binding the RNAP to the DNA, preventing ... PCC6803 can readily take up exogenous DNA, in addition to up taking DNA via electroporation, ultrasonic transformation and ... PCC 6803". DNA Research. 10 (5): 221-8. doi:10.1093/dnares/10.5.221. PMID 14686584. Shen G, Boussiba S, Vermaas WF (December ... PCC6301 ), it is more efficient to perform as much DNA cloning as possible in a fast growing host, such as Escherichia coli. In ...
Deshpande, Alok (4 January 2011). "NPCIL debunks Tiss findings on Jaitapur nuclear plant". DNA India. Retrieved 30 September ... The plant is estimated to generate 300 tonnes of used nuclear fuel each year, the volume of a 2.5 m sided cube. Owing to the ... Since the plant will use the sea water for cooling and then release warmed water in the Arabian Sea, fishermen in villages ... "Jaitapur N-plant: Ministry nod ignored creeks, trusted old data". The Times of India. Retrieved 20 April 2015. page 126. 3 Rs ...
"Plants Profile for Aquilegia barnebyi (oil shale columbine)". plants.usda.gov. Retrieved 2021-04-04. "Aquilegia barnebyi, oil ... Analysis of chloroplast DNA showed A. barnebyi is closely related to A. coerulea, a species of Aquilegia native to southern ... Plants grow 30-80 cm (12-31 in) tall, with a spread of up to 30 in (76 cm). Leaves are compound, as with other species of ... "Aquilegia barnebyi Munz". Plants of the World Online. Kew Science. Retrieved 2021-04-04. "Aquilegia barnebyi". www. ...
Mary Johnson (October 29, 2012). "VIDEO: Dramatic Explosion at East Village Con Ed Plant". DNA Info. Archived from the original ... The city lies in the USDA 7b plant hardiness zone. Winters are cold and damp, and prevailing wind patterns that blow offshore ... "USDA Plant Hardiness Zone Map". Agricultural Research Center, PRISM Climate Group Oregon State University. Archived from the ... The Croton Watershed north of the city is undergoing construction of a US$3.2 billion water purification plant to augment New ...
... which suggested that plant drought tolerance relates to the development of plant vascular tissue. Also, overexpression of ... DNA Res. 20: 315-324. Tan, B., Joseph, L. M., Deng, W., Liu, L., Li, Q., Cline, K., McCarty, D. R. 2003. Molecular ... The Plant Journal 35: 44-56. Sharp, R. E., Wu, Y., Voetberg, G. S., Saab, I. N., LeNoble, M. E. 1994. Confirmation that ... Plant Physiol. 147: 1984-1993. Barrero, J., RodrÍGuez, P. L., Quesada, V., Piqueras, P., Ponce, M. R., Micol, J. L. 2006. Both ...
Bhula, Pooja (25 February 2017). "Planting trees, saving heritage". DNA India. Retrieved 23 October 2019. "Deep inside Aarey, ... The colony also has gardens, a nursery, lakes, an observation pavilion, picnic facilities, and milk plants. 16,000 cattle are ... Gandhi, Forum (17 July 2016). "Mumbai: Historical findings give boost to Save Aarey campaign". DNA India. Retrieved 21 October ... DNA India. Retrieved 23 October 2019. "'6 new species found in Aarey in 10 years'". Hindustan Times. 19 September 2019. ...
"Plant centromeric retrotransposons: a structural and cytogenetic perspective". Mobile DNA. 2 (1): 4. doi:10.1186/1759-8753-2-4 ... Plant Sireviruses of many sequenced plant genomes are summarized at the MASIVEdb Sirevirus database. Ty3-gypsy retrotransposons ... In plant genomes, LTR retrotransposons are the major repetitive sequence class, for example, constituting more than 75% of the ... Among the TRIMs, the Cassandra family plays an exceptional role, as the family is unusually wide-spread among higher plants. In ...
Aklekar, Rajendra (26 March 2012). "Power plant for Mumbai railways gets a fillip". DNA India. Retrieved 16 July 2013. Saurabh ... The Indian Railways plans to build a 700 MW gas-based plant in Thakurli to generate electricity to run the Mumbai trains. An ... Aklekar, Rajendra (7 December 2011). "Railway think tank rules out Metro-like seats in locals". DNA India. Retrieved 16 July ... "Railways move to ensure zero mishaps". DNA India. 14 December 2011. Archived from the original on 10 January 2012. Retrieved 16 ...
"Phytohormonal responses in enod40-overexpressing plants of Medicago truncatula and rice". Physiol. Plant. 120 (1): 132-139. doi ... DNA Res. 13 (6): 255-265. doi:10.1093/dnares/dsl017. PMID 17244637. Lee A, Lum MR, Hirsch AM (2007). "ENOD40 Gene Expression ... enod40, also known as early nodulin 40, is a gene found in flowering plants. The gene has characteristics of both protein and ... In the study of non-legume plants, the over-expression of ENOD40 in transgenic Arabidopsis lines was observed a reduction of ...
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May 2010). "The Dunaliella salina organelle genomes: large sequences, inflated with intronic and intergenic DNA". BMC Plant ... "Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii". ... and comparative analysis of sequence evolution among 17 vascular plants". DNA Research. 11 (4): 247-61. doi:10.1093/dnares/11.4 ... A plastome is the genome of a plastid, a type of organelle found in plants and in a variety of protoctists. The number of known ...
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"ZF sets up Plant in Pune, India for heavy truck transmissions". machinist.in. 27 October 2010. Retrieved 19 March 2021. PTI (1 ... Tiwari, Ashish K. (6 September 2011). "Hero Group putting up a Rs650 crore hotel in Gurgaon". DNA India. Retrieved 19 March ... Das, Sohini (20 September 2017). "Munjal Kiriu Industries commissions new auto component plant in Gujarat". Business Standard ... "ZF Hero Chassis System inaugurates new plant". indiancarsbikes.in. 26 September 2015. Retrieved 19 March 2021. "Hero Motors ...
Haldia Energy Limited: A subsidiary of CESC, HEL operates two 300 MW coal based thermal power plants at Haldia in West Bengal. ... Moitra, Sumit (31 July 2014). "CESC appoints BCG to decide on power sector push". DNA India. Retrieved 21 November 2020. "Noida ... Crescent Power Limited: CPL operates a coal washery and a thermal plant near Asansol in West Bengal. Surya Vidyut Limited: The ... Dhariwal Infrastructure Limited: A subsidiary of CESC, DIL, operates two 300 MW coal based thermal power plants at Chandrapur ...
This correction is done through the use of a substitution matrix such as that derived from the Jukes-Cantor model of DNA ... Molecular Systematics of Plants II. Boston: Kluwer. pp. 265-296. ISBN 978-0-19-535668-7. Funk DJ, Omland KE (2003). "Species- ... One possible variation on this theme adjusts the rates so that overall GC content - an important measure of DNA double helix ... ISBN 978-0-19-509975-1. Wendel JF, Doyle JJ (1998). "DNA Sequencing". In Soltis DE, Soltis PS, Doyle JJ (eds.). ...
... from animals to plants". IUBMB Life. 66 (7): 462-71. doi:10.1002/iub.1290. PMID 25045044. "Entrez Gene: SLC25A1 solute carrier ... DNA Sequence. 7 (3-4): 127-39. doi:10.3109/10425179709034029. PMID 9254007. Dolce V, Cappello AR, Capobianco L (September 1997 ...
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1 DNA-Viren *1.1 Doppelsträngige DNA-Viren (dsDNA: double stranded DNA). *1.2 Einzelsträngige DNA-Viren (ssDNA: single stranded ... Virgaviridae: a new Familie of rod-shaped plant viruses. . In: Arch Virol. . 154, Nr. 12, 2009, S. 1967-72. doi:10.1007/s00705- ... Viren mit DNA-Genom bilden keine taxonomische Einheit. Doppelsträngige DNA-Viren (dsDNA: double stranded DNA)[Bearbeiten , ... Einzelsträngige DNA-Viren (ssDNA: single stranded DNA)[Bearbeiten , Quelltext bearbeiten]. Viren mit Einzelstrang-DNA-Genom ...
"European and Mediterranean Plant Protection Organization. 2010. Retrieved 6 November 2012.. *^ a b "Den senaste om ... based on morphological and DNA sequence comparisons, Chalara fraxinea was suggested to be the asexual stage (anamorph) of the ... Young and newly planted trees with the disease would be destroyed; however, mature trees would not be removed because of the ... "European and Mediterranean Plant Protection Organization. March 2012. Archived from the original on 17 July 2012. Retrieved 29 ...
DNA locus associated with variation in a quantitative trait. A quantitative trait locus (QTL) is a locus (section of DNA) that ... Jannink, J; Bink, Mc; Jansen, Rc (August 2001). "Using complex plant pedigrees to map valuable genes". Trends in Plant Science ... The DNA sequence of any genes in this region can then be compared to a database of DNA for genes whose function is already ... A quantitative trait locus (QTL) is a region of DNA which is associated with a particular phenotypic trait, which varies in ...
... adversely affecting most plants, including trees and vegetables. For plants to thrive a certain quantity of organic matter ( ... "Masanobu Fukuoka: The man who did nothing By Malvika Tegta" "DNA Daily News and Analysis". "Published: Sunday, Aug 22, 2010, 2: ... Periodically ground layer plants including weeds may be cut and left on the surface, returning their nutrients to the soil, ... Tilling uproots all the plants in the area, turning their roots into food for bacteria and fungi. This damages their ability to ...
೩.೦ ೩.೧ Simpson, Michael G. (2010). "Chapter 1 Plant Systematics: an Overview". Plant Systematics (2nd ed.). Academic Press. ... ಜೀವಶಾಸ್ತ್ರಜ್ಞರು DNA ಅನುಕ್ರಮ ಮಾಹಿತಿಯು ಬಳಸಿ ಜಾತಿವಿಕಸನೀಯ ವಿಶ್ಲೇಷಣೆ ಫಲಿತಾಂಶಗಳು ಆಧರಿಸಿದ ವರ್ಗೀಕರಣ ರೂಪಿಸಲು , ಮತ್ತು ವಿಷಗಳ ವಿಶಿಷ್ಟವಾಗಿ ... In Plant Systematics - A Phylogenetic Approach, Third Edition. Sinauer Associates, Sunderland. *↑ ... Stuessy, Tod F. (2009). Plant Taxonomy: The Systematic Evaluation of Comparative Data. Columbia University Press. ISBN 0-231- ...
December 1999). "Identification of Ebola virus sequences present as RNA or DNA in organs of terrestrial small mammals of the ... Of 24 plant and 19 vertebrate species experimentally inoculated with EBOV, only bats became infected.[86] The bats displayed no ... Plants, arthropods, rodents, and birds have also been considered possible viral reservoirs.[1][29] ... "Experimental inoculation of plants and animals with Ebola virus". Emerg. Infect. Dis. 2 (4): 321-25. doi:10.3201/ ...
... plant matter, while laying females ate 71.9% animal matter and only 28.1% plant matter.[66] Plants generally make up the larger ... Mallards are differentiated in their mitochondrial DNA between North American and Eurasian populations,[17] but the nuclear ... The mallard usually feeds by dabbling for plant food or grazing; there are reports of it eating frogs.[69] However, in 2017 a ... plant matter, most notably the grass Echinochloa crus-galli, and nonlaying females ate 37.0% animal matter and 63.0% ...
DNA studies also suggest an unknown degree of interbreeding between Homo sapiens sapiens and Homo sapiens denisova.[27] ... In general, their actual diet in the wild is about 95% plant-based, with the remaining 5% filled with insects, eggs, and baby ... Evidence from canine DNA collected by Robert K. Wayne suggests that dogs may have been first domesticated in the late Middle ... Chimpanzees are the closest to humans genetically, sharing more than 96% of their DNA code with humans, and their digestive ...
In land plants, some 11-14% of the DNA in their nuclei can be traced back to the chloroplast,[32] up to 18% in Arabidopsis, ... Introns are common in chloroplast DNA molecules, while they are rare in prokaryotic DNA molecules (plant mitochondrial DNAs ... DNA replicationEdit. Leading model of cpDNA replicationEdit. Chloroplast DNA replication via multiple D loop mechanisms. ... The 154 kb chloroplast DNA map of a model flowering plant (Arabidopsis thaliana: Brassicaceae) showing genes and inverted ...
In recognition of their major contributions to plant physiology including fundamental studies on insectivorous plants, much of ... segregation distortion and repetitive DNA. ... and for his genetic studies in animals and plants. 1932. Carl ... For his research on the population biology and evolution of plants which has greatly improved understanding of the adaptation ... On the ground of his work on scientific principles applied to the breeding of plants. ...
The DNA sequence of a gene often varies from one individual to another. Those variations are called alleles. While some genes ... for the recessive allele producing white flowers in pea plants). The genotype of an organism that is homozygous-recessive for a ... Zygosity is a description of whether those two alleles have identical or different DNA sequences. In some cases the term " ... Transgenic mice generated through exogenous DNA microinjection of an embryo's pronucleus are also considered to be hemizygous ...
This was further confirmed by molecular studies of their DNA.[6] These also showed that the brolga is more closely related to ... They also eat the shoots and leaves of wetland and upland plants, cereal grains, seeds, insects, mollusks, crustaceans, frogs ... The nest, which is built by both sexes, is a raised mound of sticks, uprooted grass, and other plant material sited on a small ... The adult diet is omnivorous and includes plant matter, invertebrates and small vertebrates. ...
"Australian Plant Name Index (APNI), IBIS database. Centre for Plant Biodiversity Research, Australian Government, Canberra. ... 2001). Chloroplast DNA phylogeny and biogeography of Lepidium (Brassicaceae). American Journal of Botany 88(11), 2051-63. ... "Sensitive Plants of the JRA" (PDF). Idaho BLM. Archived from the original (PDF) on 3 May 2014. Retrieved 2 May 2014.. ... Lepidium is a genus of plants in the mustard/cabbage family, Brassicaceae. The genus is widely distributed in the Americas, ...
For example, Agrobacterium tumefaciens is a plant pathogen, Brucella abortus is an animal pathogen, and Sinorhizobium meliloti ... In Caulobacter cells, replication of the chromosome involves about 2 million DNA synthesis reactions for each arm of the ... and the breakdown of plant-derived carbon sources, in addition to many extracytoplasmic function sigma factors, providing the ... "DnaA couples DNA replication and the expression of two cell cycle master regulators". The EMBO Journal. 25 (2): 346-56. doi ...
"DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes. 15 (4): 173-183. doi:10.1093/ ... oryzae is a harmful pathogen to either plants or animals in the scientific literature.[19] Therefore, Health Canada considers A ...
DNA Plant Technology (DNAP), Agritope and Monsanto developed tomatoes that delayed ripening by preventing the production of ... Norfolk Plant Sciences About Norfolk Plant Sciences Archived March 4, 2016, at the Wayback Machine. ... edible plants ever created.[20] Tobacco osmotic genes overexpressed in tomatoes produced plants that held a higher water ... The plant peptide hormone, systemin was first identified in tomato plants and genetic modification has been used to demonstrate ...
DNA samples of Cyanobacteria?Edit. Would Wikipedia ever collect DNA samples of simple organisms like the Cyanobacteria? - ... They are also considerad the ancestors of chloroplasts in plants and algae.. Narayanese 16:47, 30 November 2007 (UTC). Sadly, I ... "Plant" regnum doesn't mean it has become an "Animal." ...
They are gymnosperms, cone-bearing seed plants. All extant conifers are perennial woody plants with secondary growth. The great ... but recent research into DNA sequences suggests that this interpretation leaves the Pinales without Taxales as paraphyletic, ... Seed germinates and seedling grows into a mature plant.. *When the plant is mature, it produces cones and the cycle continues. ... Plants with unusual growth habits, sizes, and colours are propagated and planted in parks and gardens throughout the world.[25] ...
"The Origin and Establishment of the Plastid in Algae and Plants". Annual Review of Genetics. 41 (1): 147-68. doi:10.1146/ ... not recognized by host spliceosomes because they are too small and therefore cannot be cut and later incorporated into host DNA ...
Usually organisms that have a higher rate of reproduction than their competitors have an evolutionary advantage. Consequently, organisms can evolve to become simpler and thus multiply faster and produce more offspring, as they require fewer resources to reproduce. A good example are parasites such as Plasmodium - the parasite responsible for malaria - and mycoplasma; these organisms often dispense with traits that are made unnecessary through parasitism on a host.[7] A lineage can also dispense with complexity when a particular complex trait merely provides no selective advantage in a particular environment. Loss of this trait need not necessarily confer a selective advantage, but may be lost due to the accumulation of mutations if its loss does not confer an immediate selective disadvantage.[8] For example, a parasitic organism may dispense with the synthetic pathway of a metabolite where it can readily scavenge that metabolite from its host. Discarding this synthesis may not necessarily allow ...
Their DNA is slightly less than one percent different from humans, and they age at a much slower rate. Since ninety-seven ... Sophia informs President Martinez that she believes Thomas intends to steal uranium from a nuclear power plant to open a portal ... President Martinez becomes suspicious of his wife's refusal to take the DNA test. Vice President Jarvis meets Sophia with a ... Special Forces teams take down the Sleepers at the food processing plant and at the engraving bureau. Sean, Vicky, Sterling, ...
The examination of preserved museum specimens has found Borrelia DNA in an infected Ixodes ricinus tick from Germany that dates ... ornamental plants and perennial groundcover (about a quarter), and lawns (about 30 times less).[130] Ixodes larvae and nymphs ... Except for one study in Europe,[229] much of the data implicating lizards is based on DNA detection of the spirochete and has ... Polymerase chain reaction (PCR) tests for Lyme disease have also been developed to detect the genetic material (DNA) of the ...
During fertilization this combines with the DNA that is in the egg of the female part and a zygote is formed. In seed plants a ... In plants it is a double fertilisation in which two sperm cells fertilize cells in the plant ovary. One of these is a normal ... Each pollen grain is haploid: it has half of the DNA (genetic information) that is needed to make a new plant. ... Pollination is part of sexual reproduction in plants. It describes how the pollen grains get to the female parts of a plant. ...
Darwin, Charles (1868). The Variation of Animals and Plants under Domestication. Volume 1 (1st ed.). London: John Murray. pp. ... However, DNA study shows that "the dingo originates from domesticated dogs, originally from East Asia"[16] (which reverted to ... DNA analysis consistently shows that all existing red wolves carry coyote genes. This has caused a problem for Canid taxonomy, ... A study of the maternal mitochondrial DNA of the black-backed jackal could find no evidence of genotypes from the most likely ...
First plant genome sequenced, Dec 2000.[9] Plant, Genlisea margaretae 6.34×107 Smallest recorded flowering plant genome, 2006.[ ... The genome of an organism is the whole of its hereditary information encoded in its DNA (or, for some viruses, RNA). This ... Plant Biology. 8: 770-777. *↑ The C. elegans sequencing consortium (1998). "Genome sequence of the nematode C. elegans: a ... Note: The DNA from a single human cell has a length of ~1.8 m (but at a width of ~2.4 nanometers). ...
"The Plant Cell. 9 (10): 1767-1780. doi:10.1105/tpc.9.10.1767. PMC 157020. PMID 12237347.. ... "Scientific Opinion on the substantiation of health claims related to quercetin and protection of DNA, proteins and lipids from ... "Plant Physiology. 126 (2): 485-93. doi:10.1104/pp.126.2.485. PMC 1540115. PMID 11402179.. ... Quercetin, a plant flavonol from the flavonoid group of polyphenols, is found in many fruits, vegetables, leaves, and grains; ...
The success rate of usable DNA extraction is relatively high in paleofeces, making it more reliable than skeletal DNA retrieval ... An advantage for a plant in having fruit is that animals will eat the fruit and unknowingly disperse the seed in doing so. This ... Stokstad, Erik (28 July 2000). "Divining Diet and Disease From DNA". Science. 289 (5479): 530-531. doi:10.1126/science.289.5479 ... "Panda Poop Might Help Turn Plants Into Fuel". News.nationalgeographic.com. 2013-09-10. Retrieved 2013-10-02.. ...
These inhibit the germination of most competing plants and kill beneficial soil fungi needed by many plants, such as many tree ... Early DNA-analysis showed that the Capparaceae-as defined at that moment-were paraphyletic, and it was suggested to assign the ... "The Plant List.. *^ Turini TA, Daugovish O, Koike ST, Natwick ET, Ploeg A, Dara SK, Fennimore SA, Joseph S, LeStrange M, Smith ... "Frontiers in Plant Science. 7 (451): 451. doi:10.3389/fpls.2016.00451. PMC 4824781. PMID 27092164.. ...
In plant breeding, inbred lines are used as stocks for the creation of hybrid lines to make use of the effects of heterosis. ... Gilbert DA, Packer C, Pusey AE, Stephens JC, O'Brien SJ (1991-10-01). "Analytical DNA fingerprinting in lions: parentage, ... Inbreeding in plants also occurs naturally in the form of self-pollination. ... Plant Breeding. 117 (5): 429-35. doi:10.1111/j.1439-0523.1998.tb01968.x. hdl:10261/121301.. ...
"More DNA support for a Cetacea/Hippopotamidae clade: the blood-clotting protein gene gamma-fibrinogen" (PDF). Molecular ... Plant epithet. *Sacred grove *In India. *Sacred plants *Bodhi Tree. *Lime tree ...
... Richard Dana rcdana at crash.cts.com Thu Jun 16 05:16:37 EST 1994 *Previous message: hygromycin phosphotransferase ... Request for plant DNA methods from diverse species and different structures. Purpose of Project Green Gene is to isolate and ... preserve DNA and cDNA libraries from diverse plant species. These libraries are to then be supplied to researchers and the DNA ... DNA methods, RNAID methods, first stand systhesis methods. Please help. *Previous message: hygromycin phosphotransferase ...
But one European luxury goods company has decided to fight back by putting plant DNA markers in its products to identify the ... Plant DNA Helps Catch Criminals and Thwart Counterfeiters. Engineered plant DNA strands inserted in luxury goods give law ... Applied DNA whips up specially tailored plant DNA that can be used in fabrics, dyes or glues to make each product genetically ... But one European luxury goods company has decided to fight back by putting plant DNA markers in its products to identify the ...
Gel mobility shift experiments employing ten different recombinant plant bZIP proteins demonstrated that nucleotides flanking ... Plant bZIP proteins exhibit a relaxed DNA-binding specificity for DNA sequence motifs containing an ACGT core. ... Plant bZIP proteins exhibit a relaxed DNA-binding specificity for DNA sequence motifs containing an ACGT core. Gel mobility ... Plant bZIP protein DNA binding specificity J Mol Biol. 1993 Apr 20;230(4):1131-44. doi: 10.1006/jmbi.1993.1230. ...
Only 3% of this aquatic plants DNA is not part of a known gene, new research shows. In contrast, only 2% of human DNA is part ... Only 3% of this aquatic plants DNA is not part of a known gene, new research shows. In contrast, only 2% of human DNA is part ... DNA replication requires quite a bit of nitrogen, so less DNA means the plant needs less nitrogen to copy a cell. ... Only 3% of this aquatic plants DNA is not part of a known gene, new research shows. In contrast, only 2% of human DNA is part ...
Now, theyre considering a novel approach to give legit wares a mark of distinction: embed them with strands of plant DNA. ... have figured out how to create unique DNA signatures out of plant genomes. A DNA-marked coating can then be applied to just ... Now, theyre considering a novel approach to give legit wares a mark of distinction: embed them with strands of plant DNA. ... Now, theyre considering a novel approach to give legit wares a mark of distinction: embed them with strands of plant DNA. ...
Nuclear DNA was first edited in the early 1970s, chloroplast DNA was first edited in 1988, and animal mitochondrial DNA was ... However, no tool previously successfully edited plant mitochondrial DNA. Researchers used their technique to create four new ... Researchers in Japan have edited plant mitochondrial DNA for the first time, which could lead to a more secure food supply. ... Researchers can finally modify plant mitochondrial DNA. University of Tokyo. Journal. Nature Plants. Funder. Japanese Science ...
Junk DNA is probably well named as junk. There doesn¿t seem to be any glorious reason or function behind it, said Victor ... The rest of it is non-coding DNA. ... The carnivorous bladderwort plant, Utricularia gibba, has only ... Do humans need mystery junk DNA? This carnivorous plant doesnt A scanning electron micrograph shows a tinted image of the ... And yet the plant packs efficiently, stuffing all its useful genetic code into a fraction of the sprawling DNA real estate ...
... automated purification of genomic DNA (gDNA) from plant tissue samples. ... The Maxwell 16 LEV Plant DNA Kit is used with the Maxwell 16 Research Instrument (Cat.# AS2000) to provide an easy method for ... Plant DNA Kits is ready for use in downstream applications, including qPCR. PCR using DNA purified with the Maxwell® Plant DNA ... The Maxwell® Plant DNA Kits rapidly produce high-quality DNA for use in sequencing and qPCR-based applications in plant ...
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... plant DNA. This article at Wired discusses how plant DNA can be used to make an almost unique code (1 in 1 trillion) for ... ... Highlighting another unique way to use cutting edge DNA technology, the U.S. Department of Defense has a new weapon in its ... plant DNA. This article at Wired discusses how plant DNA can be used to make an almost unique code (1 in 1 trillion) for parts ... Salmon DNA Used In Data Storage Device. Submission: DoD Using Plant DNA to Combat Counterfeit Parts GAO Sting Finds More Fake ...
... comprehensive guide to the structure of DNA systems and their biochemical application. Carefully compiled and filled with a ... Plant DNA: Contents and Systematics. 2. Repeated DNA Sequences and Polyploidy in Cereal Crops. 3. Homology of Nonrepeated DNA ... Conserved and Diverged DNA Sequences. 8. A Critical Review of Some Terminologies Used for Additional DNA in Plant Chromosomes. ... Volume I. 1. The Role of the Computer in Estimates of DNA Nucleotide Sequence Divergence. 2. Mobile DNA Sequences and Their ...
WT-DNA,. wild-type DNA;. MT-DNA,. mutant DNA;. BD,. binding domain;. HD,. homeodomain;. AD,. activation domain;. SAAB,. ... A) WT-DNA and MT-DNA binding sites were used as probes. (B) T7-KN1 was incubated with WT-DNA probe (W) or MT-DNA probe (M). (C ... In EMSA, recombinant expressed and purified proteins were mixed with 50,000 cpm of wild-type DNA (WT-DNA) or mutant DNA (MT-DNA ... DNA-binding specificity was determined by competing with unlabeled WT-DNA (wt comp) or MT-DNA (mt comp), at 10- and 100-fold ...
... which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon ... Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific ... conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said ... class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. ...
DNA barcoding of medicinal plant material for identification.. Techen N1, Parveen I1, Pan Z2, Khan IA3. ... The database should provide DNA barcodes for data retrieval and similarity search. In order to obtain such barcodes, several ... it is vital to provide a single database containing information about authentic plant materials and their potential adulterants ... molecular methods have been applied to develop markers that aid with the authentication and identification of medicinal plant ...
DNA Analysis. New DNA extractions were performed with the DNeasy Plant Mini kit (Qiagen, Valencia, CA) after tissue disruption ... 15). Among plants, especially angiosperms, DNA-based identifications, although not strictly through the use of DNA barcodes, ... Use of DNA barcodes to identify flowering plants. W. John Kress, Kenneth J. Wurdack, Elizabeth A. Zimmer, Lee A. Weigt, Daniel ... Use of DNA barcodes to identify flowering plants. W. John Kress, Kenneth J. Wurdack, Elizabeth A. Zimmer, Lee A. Weigt, Daniel ...
... tarantulito tarantulito at hotmail.com Mon Feb 15 13:11:37 EST 1999 * ... john rascoe department of entomology and plant pathology 110 noble research center, OSU stillwater, ok 74078 *Previous message ... i am attempting an experiment to cut time and expense in processing large numbers of small-diameter plant stems for subsequent ...
The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. ... The GenElute Plant Genomic DNA Miniprep Kit enables the purification of high-quality genomic DNA from a variety of plant ... Genomic DNA Isolation from Rice (GenElute Plant Genomic DNA Kit). Genomic DNA Isolation from Cotton (GenElute Plant Genomic DNA ... Description: With the GenElute Plant Genomic DNA Miniprep Kit, high quality genomic DNA can be purified from a variety of plant ...
DNA viruses have evolved very different replication strategies as well as a rich variety of molecular interactions with their ... The main stages constituting the replicative cycle of a DNA virus are depicted. In the case of plant DNA viruses, there is a ... DNA Plant and Animal Virus Replication. Crisanto Gutierrez, Centro de Biologia Molecular Severo Ochoa, Consejo Superior de ... Gutierrez, Crisanto, and Martinez‐Salas, Encarnacion(Dec 2001) DNA Plant and Animal Virus Replication. In: eLS. John Wiley & ...
... system works much more efficiently in the active genes of plants as compared to humans. And this efficiency depends on the day/ ... instead they have a super robust DNA repair kit to combat UV radiation. Today, the lab of 2015 Nobel laureate Aziz Sancar ... Plants cant come in from the sun or slather on sunblock; ... how-does-plant-dna-avoid-the-ravages-of-uv-radiation More in ... does UV radiation also damage plant DNA? The answer is yes, but because plants cant come in from the sun or slather on ...
Plant DNA barcoding research is shifting beyond performance comparisons of different DNA regions towards practical applications ... Plant Barcoding China: DNA barcoding of 5000 Chinese plant species Kunming Institute of Botany• All-genera: DNA barcoding of ... DNA barcoding Chinese medicinal plants :Institute of Medicinal Plant Development Beijing• DNA barcoding the flora of Wales : ... Use of DNA barcoding and its role in the plant species/varietal Identification * 1. DNA Barcode: species/varietal ...
To make the DNA tags scientists extract genetic code from plants and reassemble it to be distinct from any other DNA on the ... Authentic Drugs Tagged with Plant DNA Could Help Snare Fake Meds. A new high-tech tactic could help officials detect ... Applied DNA Sciences calls its technique SigNature DNA, which could be used in myriad products in addition to pharmaceuticals. ... Now a biotech company called Applied DNA Sciences may have an original solution: tagging legitimate drugs with engineered DNA. ...
In 2002, Bionova shut down DNA Plant Technology. DNA Plant Technology was founded in 1981 by Dr. William R. Sharp and Dr. David ... "DNA Plant Technology unveils second-generation genetically-modified tomato". "Dna Plant Technology Completes Sale Of Frost ... which had been carried out through its DNA Plant Technology subsidiary. In 1991, DNA Plant Technology applied for and were ... Key experiments in the control of plant transgene expression were performed by Jorgensen after he joined DNA Plant Technology ...
... Plant DNA Barcoding Publications. García-Robledo, Carlos, Erickson, David L., Staines, Charles L., ... Generating Plant DNA Barcodes for Trees in Long-Term Forest Dynamics Plots. In: Kress, W. J. and Erickson, David L., DNA ... A DNA barcode for land plants. Proceedings of the National Academy of Sciences of the United States of America, 106(31): 12794- ... Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences of the United States of ...
The T-DNA of the BIBAC vector can be transferred into the plant nuclear genome. As examples, a 30-kb yeast genomic DNA fragment ... A binary-BAC system for plant transformation with high-molecular-weight DNA.. Hamilton CM1. ... A binary-BAC (BIBAC) vector suitable for Agrobacterium-mediated plant transformation with high-molecular-weight DNA was ... Plant Science Center, Cornell University, Ithaca, NY 14853, USA. [email protected] ...
... to orchestrate the cellular processes required to protect and repair the genome in response to DNA damage. The research, ... reveals the genetic framework controlling a complex biological process that has broad implications for understanding how plants ... in particular, and organisms in general, cope with DNA damage to ensure long-term health and fitness. ... To repair DNA damage, plants need good contractors Salk scientists reveal a complex network of genes that helps plants cope ...
Plant DNA extraction - DNA extraction (Jul/12/2002 ). Hi, I am a Biochem student. I am extracting DNA from plants that have ... This will minimize DNase activity in the plant tissue before you extract the DNA.. AronD ... As long as you handle the plant tissue properly, there should be no difference between your fresh and dried samples. This means ...
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... 24.02.2010. The origins of flowering plants from peas to oak trees ... DNA »Evolutionary Genetics »Peas »Pentapetalae »flowering plant »genetic analyses »genome sequence »hibiscus »molecular ... Further reports about: , DNA , Evolutionary Genetics , Peas , Pentapetalae , flowering plant , genetic analyses , genome ... Genome sequencing is more time-consuming for plants than animals because plastid DNA is about 10 times larger than the ...
There have been continuous modification and standardization in DNA isolation protocols. Most of the plant DNA isolation ... A review of the chemicals used in the CTAB method of DNA extraction and their probable functions on the highly evolved yet ... CTAB, NaCl, PVP, ethanol, and isopropanol) used during the DNA extraction procedure will benefit to set or modify protocols for ... Modification is usually performed in the concentration of chemicals used during the extraction procedure according to the plant ...
The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochond … ... A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is ... Rapid isolation of high molecular weight plant DNA Nucleic Acids Res. 1980 Oct 10;8(19):4321-5. doi: 10.1093/nar/8.19.4321. ... A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is ...
  • Evidence for localized selection on the U. gibba gene complement, however, does not provide support for the existence of genome-wide selective forces that might favour reduction of nonessential, non-coding DNA. (slashdot.org)
  • The plant mitochondrial genome is huge in comparison, the structure is much more complicated, the genes are sometimes duplicated, the gene expression mechanisms are not well-understood, and some mitochondria have no genomes at all - in our previous studies, we observed that they fuse with other mitochondria to exchange protein products and then separate again," said Arimura. (eurekalert.org)
  • New research into the plant's genome shows that it contains almost no noncoding DNA -- demonstrating that a complex life form can function without the so-called junk DNA. (latimes.com)
  • Repeated segments buried in the plant's DNA show them that the entire genome has been duplicated three times since its lineage split off from its common ancestor with the tomato and the grape -- and yet this regular doubling of the code hasn't increased its length. (latimes.com)
  • My reading of the simplest version of this is that they take some target DNA (e.g. derived from a plant genome, and possibly cut up and re-ligated to swap things around), and design a single 'forward' PCR primer and multiple 'reverse' primers that bind the target sequence at various positions. (slashdot.org)
  • Examples are given for viruses with a single‐stranded DNA genome, either (a) linear ( Parvoviridae , genus Dependovirus ) or (b) circular ( Geminiviridae , genus Mastrevirus ), and with a double‐stranded DNA genome, either (c) linear ( Adenoviridae , genus Mastadenovirus ) or (d) circular ( Papovaviridae , genus Polyomavirus ). (els.net)
  • The sequences of these DNA snippets can be matched to corresponding stretches of DNA on a reference genome, in order to map precisely the spots where DNA-damage is under repair. (eurekalert.org)
  • One is that knocking out the excision repair system leads to an increase in plant genome mutations even when the plant is kept in the dark, away from UV or other forms of light. (eurekalert.org)
  • Researchers sequenced the genome of the gardenia, then looked at how the plant makes a compound called crocin. (nsf.gov)
  • The T-DNA of the BIBAC vector can be transferred into the plant nuclear genome. (nih.gov)
  • Similarly, when DNA is damaged, a molecular general contractor oversees a network of genetic subcontractors to ensure that the diverse cellular tasks needed to protect and repair the genome are carried out correctly and on time. (eurekalert.org)
  • Now, researchers at the Salk Institute report which genes are turned on or off, and in which order, to orchestrate the cellular processes required to protect and repair the genome in response to DNA damage. (eurekalert.org)
  • This paper brings us one step closer to understanding how the response to DNA damage is coordinated over time to maintain genome stability. (eurekalert.org)
  • In addition to informing strategies to improve crop health by maintaining genome stability, this work may also shed light on conserved aspects of the DNA damage response in other organisms, as there are many parallels between SOG1 and a gene in animals that has a similar "general contractor" function--the p53 gene, a tumor suppressor known for its role in combating DNA damage to prevent cancer. (eurekalert.org)
  • The new study at UF's Florida Museum of Natural History analyzed 86 complete plastid genome sequences from a wide range of plant species. (innovations-report.com)
  • Genome sequencing is more time-consuming for plants than animals because plastid DNA is about 10 times larger than the mitochondrial DNA used in studying animal genomes. (innovations-report.com)
  • The Plant DNA C-values Database (https://cvalues.science.kew.org/) is a comprehensive catalogue of C-value (nuclear DNA content, or in diploids, genome size) data for land plants and algae. (wikipedia.org)
  • 2005. Genome size evolution in plants. (wikipedia.org)
  • 2014. Recent updates and developments to plant genome size databases. (wikipedia.org)
  • Researchers at University's Arabidopsis cDNA Sequence Analysis Project are mapping the plant's genome to create a model to better understand genetic data from other plants. (mndaily.com)
  • Every cell in a living organism contains a genome, which consists of genetic information coded in a double helix strand of the chemical DNA. (mndaily.com)
  • In conjunction with an enhanced system for Agrobacterium-mediated plant transformation, a new binary bacterial artificial chromosome (BIBAC) vector has been developed that is capable of transferring at least 150 kb of foreign DNA into a plant nuclear genome. (rti.org)
  • World's Largest Genome Belongs to Slow Growing Mountain Flower http://www.telegraph.co.uk/news/science/science-news/8196572/Worlds-largest-genome-belongs-to-slow-growing-mountain-flower .html "It is an unremarkable and rather fragile plant, but a flowering herb that frustrates gardeners for being notoriously difficult to grow has stunned scientists after they discovered it has the world's largest genome. (wn.com)
  • The bacterium Agrobacterium tumefaciens has been the workhorse in plant genome engineering. (jcvi.org)
  • Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant genome. (jcvi.org)
  • In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. (plantcell.org)
  • Loss-of-function mutation in DDB2 leads to genome-wide DNA methylation alterations. (plantcell.org)
  • NER comprises two subpathways, global genome repair ( GGR ) and transcription-coupled repair, both of which process bulky DNA lesions throughout the genome or along the transcribed strand of active genes, respectively ( Schärer, 2013 ). (plantcell.org)
  • Our results show that particle acceleration can be used for the introduction of foreign DNA into the soybean genome and indicate the technique may be useful in the recovery of engineered plants by transformation of regenerable tissues. (plantphysiol.org)
  • Sysmex has launched two new reagent kits - CyStain™ UV OxProtect and CyStain™ PI OxProtect - to determine ploidy content and genome size in plants that are sensitive to oxidative processes. (selectscience.net)
  • Seeking to catch an arms-race maneuver in action, researchers have uncovered new evidence to explain how bacteria in the process of infecting a plant can shift molecular gears by excising specific genes from its genome to overcome the host plant's specific defenses. (bio-medicine.org)
  • Studying interactions between strains of the halo-bright pathogen and bean plants, the researchers found that the pathogenic bacteria essentially kicks out a section of its genome when it senses that its presence has been detected by the plant's defense system. (bio-medicine.org)
  • In their study, the authors identify within the halo-bright pathogen genome a special island of DNA that encodes one such offending protein. (bio-medicine.org)
  • But this genomic island also encodes enzymes that, when switched on, snip the DNA on either side of the island, resulting in the excision of the entire island from the genome. (bio-medicine.org)
  • Gardenia's newly sequenced genome highlights how evolutionary tinkering transforms plants into some of nature's great chemical-makers. (scitechdaily.com)
  • It was exciting to uncover these molecular 'tricks of the trade' while researching the genome of a plant so important to traditional Chinese medicine, and now to modern biomedical research as well," says the study's co-corresponding author, Jingyuan Song, PhD, from the Engineering Research Center of Chinese Medicine Resource in China, who is also affiliated with the Chinese Academy of Medical Sciences and Peking Union Medical College. (scitechdaily.com)
  • A prime example is the use of short, standardized regions of the genome as taxon barcodes for biological identification of plants. (springer.com)
  • The routine generation of transgenic plants involves analysis of transgene integration into the host genome as well as the evaluation of the transgene expression using standard molecular techniques such as polymerase chain reaction (PCR), Southern blot, reverse transcription PCR (RT-PCR) and Enzyme Linked Immunosorbent Assay (ELISA) or Western blot. (ejbiotechnology.info)
  • It's another to come up with bogus DNA that's a perfect copy of the original, or try to tamper with the complex sequences of base pairs that comprise a single DNA strand. (wired.com)
  • 2. Mobile DNA Sequences and Their Possible Role in Evolution. (crcpress.com)
  • 2. Repeated DNA Sequences and Polyploidy in Cereal Crops. (crcpress.com)
  • 3. Homology of Nonrepeated DNA Sequences in Phylogeny of Fungal Species. (crcpress.com)
  • 7. Molecular Analysis of Plant Genomes: Conserved and Diverged DNA Sequences. (crcpress.com)
  • however, the affinity is greatly increased when they cooperatively bind to their appropriate DNA sequences as a heterodimer ( 17 - 21 , 26 ). (pnas.org)
  • 2. A recombinant DNA molecule according to claim 1 , wherein the said DNA comprises a chimaeric DNA construct comprising an expressible DNA in operable linkage with expression signals active in plant cells, wherein said expression signals are selected from the group consisting of promoter and termination sequences. (google.com)
  • 10. The recombinant DNA molecule according to claim 2 , wherein said DNA further comprises coding sequences of the 5′ region, 3′ region, or 5′ and 3′ region. (google.com)
  • Methods for identifying species by using short orthologous DNA sequences, known as "DNA barcodes," have been proposed and initiated to facilitate biodiversity studies, identify juveniles, associate sexes, and enhance forensic analyses. (pnas.org)
  • Engineered DNA sequences also have been suggested as exact identifiers and intellectual property tags for transgenic organisms ( 6 ). (pnas.org)
  • The main aim of DNA barcoding is to establish a shared community resource of DNA sequences that can be used for organismal identification and taxonomic clarification. (slideshare.net)
  • About 35 percent cDNA sequences for plants match those for animals. (mndaily.com)
  • The authors show that when AP1 proteins interact with each other to form AP1-AP1 homodimers, they bind to different DNA sequences than AP1-SEP3 heterodimers. (plantcell.org)
  • Similarly, AG-AG homodimers bind to different DNA sequences than AG-SEP3 heterodimers. (plantcell.org)
  • In addition, the degree of DNA bending intrinsic to these sequences influences their interactions with heterodimers. (plantcell.org)
  • DNA specificity plot comparing the relative binding affinities of SELEX-seq sequences selected by SEP3-AG ( y axis) and SEP3-AP1 ( x axis). (plantcell.org)
  • Although the correlation between the authors' in vitro data and previously published in vivo data was significant, several hundred DNA sequences interact with these transcription factors in vivo, which, as acknowledged by the authors, were not explained by SEP3 heterodimerization. (plantcell.org)
  • Male-associated DNA sequences were analyzed in a dioecious plant, Cannabis sativa L. (family: Moraceae), which is known to have sex chromosomes. (nii.ac.jp)
  • A high percentage of the differences in the DNA sequences is due to the extremely strong bias in the corn gene to have a G/C base in the third codon position with 559/569 codons ending in a G or C. Using a hydroponic system, maize seedlings grown in the absence of an exogenous nitrogen source were induced with nitrate or nitrite. (plantphysiol.org)
  • The team behind the discovery found that DNA sequences of the gene 'ma. (bio-medicine.org)
  • The team behind the discovery found that DNA sequences of the gene 'matK' differ among plant species, but are nearly identical in plants of the same species. (bio-medicine.org)
  • PHILADELPHIA - DNA sequences between mitochondria within a single cell are vastly different, found researchers in the Perelman School of Medicine at the University of Pennsylvania . (pennmedicine.org)
  • Eukaryotic genomes contain a large proportion of repetitive DNA sequences, mostly transposable elements (TEs) and tandem repeats. (biomedsearch.com)
  • In non-recombining regions of the Y chromosome repetitive DNA sequences are accumulated, representing a dominant and early process forming the Y chromosome, probably before genes start to degenerate. (biomedsearch.com)
  • Working with the Sequences, Maps and Bacterial Artificial Chromosome (BAC) Libraries Program and the ENCODE (ENCyclopedia Of DNA Elements) project. (genome.gov)
  • Most recently, in April 2010, scientists from Italy reported DNA sequences stemming from genetically engineered soy in milk from goats. (testbiotech.org)
  • They also allow isolation of low-abundance chromatin proteins and confirmation that proteins of interest are associated with specific DNA sequences, for example telomeric tracts. (muni.cz)
  • The complex III, in addition to binding to telomeric sequences, has a binding affinity to rice nuclear RNA, whereas two other complexes have a binding affinity to only single-stranded G-rich telomere DNA. (deepdyve.com)
  • Our study generated DNA sequences from a vast number of distantly related plants, and we developed new analysis tools to understand their relationships and the timing of key innovations in plant evolution,' said Jim Leebens-Mack, associate professor of plant biology at the University of Georgia and coordinating author of the paper. (umich.edu)
  • As part of the One Thousand Plants (1KP) initiative, the research team is generating millions of gene sequences from plant species sampled from across the green tree of life. (umich.edu)
  • Request for plant DNA methods from diverse species and different structures. (bio.net)
  • Purpose of Project Green Gene is to isolate and preserve DNA and cDNA libraries from diverse plant species. (bio.net)
  • Even without the extreme examples such as bladderwort we readily observe 10x variability in the amount of DNA between fairly recently separated species. (slashdot.org)
  • The cytochrome c oxidase 1 sequence, which has been found to be widely applicable in animal barcoding, is not appropriate for most species of plants because of a much slower rate of cytochrome c oxidase 1 gene evolution in higher plants than in animals. (pnas.org)
  • The internal transcribed spacer is the most commonly sequenced locus used in plant phylogenetic investigations at the species level and shows high levels of interspecific divergence. (pnas.org)
  • These "DNA barcodes" show promise in providing a practical, standardized, species-level identification tool that can be used for biodiversity assessment, life history and ecological studies, and forensic analysis. (pnas.org)
  • DNA barcoding follows the same principle as does the basic taxonomic practice of associating a name with a specific reference collection in conjunction with a functional understanding of species concepts (i.e., interpreting discontinuities in interspecific variation). (pnas.org)
  • Among plants, especially angiosperms, DNA-based identifications, although not strictly through the use of DNA barcodes, have been creatively used to reconstruct extinct herbivore diets ( 16 , 17 ), to identify species of wood ( 18 ), to correlate roots growing in Texas caves with the surface flora ( 19 ), and to determine species used in herbal supplements ( 20 ). (pnas.org)
  • With the GenElute Plant Genomic DNA Miniprep Kit, high quality genomic DNA can be purified from a variety of plant species ( Table 1 , Figure 1 , and Figure 2 ). (sigmaaldrich.com)
  • Genomic DNA from various plant species isolated with GenElute Plant Genomic DNA Miniprep Kit. (sigmaaldrich.com)
  • The studies on cucumis sp for the application of DNA barcode shows the possibility of discrimination at species level not the varietal level using the matK gene barcode. (slideshare.net)
  • 2013. Tropical Plant-Herbivore Networks: Reconstructing Species Interactions Using DNA Barcodes. (si.edu)
  • 2011. Biodiversity studies in Phaseolus species by DNA barcoding. (si.edu)
  • Understanding how these plants are related is a large undertaking that could help ecologists better understand which species are more vulnerable to environmental factors such as climate change. (innovations-report.com)
  • This study shows how those species are related and sheds further light on the emergence of flowering plants, an evolutionary phenomenon described by Charles Darwin as an abominable mystery. (innovations-report.com)
  • Previous genetic analyses of Pentapetalae failed to untangle the relationships among living species, suggesting that the plants diverged rapidly over 5 million years. (innovations-report.com)
  • Various plant species are biochemically heterogeneous in nature, a single deoxyribose nucleic acid (DNA) isolation protocol may not be suitable. (intechopen.com)
  • Modification is usually performed in the concentration of chemicals used during the extraction procedure according to the plant species and plant part used. (intechopen.com)
  • The amount of these components varies according to plant species, plant part used, environmental condition, and growth stage and it is very problematic when isolating DNA. (intechopen.com)
  • The technique is ideal for the rapid isolation of small amounts of DNA from many different species and is also useful for large scale isolations. (nih.gov)
  • It contains data for 12,273 species of plants comprising 10,770 angiosperms, 421 gymnosperms, 303 pteridophytes (246 ferns and fern allies and 57 lycophytes), 334 bryophytes, and 445 algae. (wikipedia.org)
  • The DNA damage profiles for the ryegrasses differed from the radish profiles, indicating that nanoparticle-induced DNA damage is dependent on the plant species and on the nanoparticle concentration. (innovations-report.com)
  • Finally, the researchers showed that cupric oxide nanoparticles had a significant effect on growth, stunting the development of both roots and shoots in all three plant species tested. (innovations-report.com)
  • 1983. An effective method of DNA isolation from the mature leaves of Gossypium species that contain large amounts of phenolic terpenoids and tannins. (springer.com)
  • Success of species assignment using DNA barcodes has been shown to vary among plant lineages because of a wide range of different factors. (wiley.com)
  • Combining plant DNA is possible by grafting or cross-pollinating two different species of plant to create a new species. (wn.com)
  • However, since Goji is an umbrella term for different plant species that are closely related, mislabeling and adulterations (unconsciously or purposely) are possible. (frontiersin.org)
  • Goji" is the generic name for different plant species from the genus Lycium , belonging to the Solanaceae family. (frontiersin.org)
  • It was these species-specific preferences that Daniell's team sought to exploit in a recent paper published in the journal Plant Physiology . (phys.org)
  • The researchers analyzed the genomes of 133 plant species to see which codons were used most frequently to code for particular amino acids. (phys.org)
  • A 'barcode' gene that can be used to distinguish between the majority of plant species on Earth has been identified by scientists who publish their findings in the Proceedings of the National Academy of Sciences journal today (Monday 4 February 2008). (bio-medicine.org)
  • This gene, which can be used to identify plants using a small sample, could lead to new ways of easily cataloguing different types of plants in species-rich areas like rainforests. (bio-medicine.org)
  • It could also lead to accurate methods for identifying plant ingredients in powdered substances, such as in traditional Chinese medicines, and could help to monitor and prevent the illegal transportation of endangered plant species. (bio-medicine.org)
  • This means that the matK gene can provide scientists with an easy way of distinguishing between different plants, even closely related species that may look the same to the human eye. (bio-medicine.org)
  • They found that when one plant species was closely related to another, differences were usually detected in the matK DNA. (bio-medicine.org)
  • Dr Savolainen explains that in the long run the aim is to build on the genetic information his team gathered from Costa Rica and South Africa to create a genetic database of the matK DNA of as many plant species as possible, so that samples can be compared to this database and different species accurately identified. (bio-medicine.org)
  • The matK gene may not, however, be able to be used to identify every plant species on Earth. (bio-medicine.org)
  • CyStain™ UV / PI OxProtect are designed for the analysis of DNA in plant species where DNA staining with regular reagents can be difficult because of the presence of oxidative substances. (selectscience.net)
  • An international team of scientists has mapped the evolutionary relationships between China's 30,000 flowering plant species, uncovering a distinct regional pattern in biodiversity. (alligator.org)
  • Here we review the occurrence and role of repetitive DNA in Y chromosome evolution in various species with a focus on dioecious plants. (biomedsearch.com)
  • We have expertise in extracting nucleic acid material from over 100 different species and tissue types, and our scientists are ready to work with you on obtaining high quality DNA extractions from your toughest samples. (biosearchtech.com)
  • 24-nt small interfering RNA (siRNA) are known to mediate gene inactivation via the RNA-directed DNA methylation pathway (RdDM) and are important for natural heritable changes in plant species. (scirp.org)
  • Then, as a species evolves over time, the excess DNA is free to mutate and take on new functions. (scitechdaily.com)
  • A species specific satellite DNA of Drosophila guanche , Z. (springer.com)
  • Burrows, P.R., 1988, The differentiation of Globodera pallida from G. rostochiensis using species-specific DNA probes, Nematologica (Abstract. (springer.com)
  • Per P.M. Hollingsworth, DNA bar-coding plants in biodiversity hot spots: Progress and outstanding questions (Heredity, 9 April 2008) "DNA bar-coding is now routinely used for organismal identification" in animals and "has contributed to the discovery of new species. (colorbasepair.com)
  • Their research found that the matK gene (which "contained significant species-level genetic variability and divergence, conserved flanking sites for developing PCR (polymerase chain reaction, a process that enables scientists to produce millions of copies of a specific DNA sequence in about two hours while bypassing the need to use bacteria to amplify DNA) primers for wide taxonomic application, [and] a short sequence length. (colorbasepair.com)
  • Dolan DNA Learning Center and Cold Spring Harbor Laboratory, Inc. 2009) could be used to differentiate between at least 90% of all plants, including those that appeared identical to the human eye, known as cryptic species because of their identical appearance and genetic differences. (colorbasepair.com)
  • However, consistent with mitochronidrial genes in animals, "chloroplast genes [in plants] evolve at a slower rate, allowing for [distinguishment between the same species, and] fast enough for differences to occur in the DNA code between species. (colorbasepair.com)
  • This kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungal species. (norgenbiotek.com)
  • The E.Z.N.A.® Plant DNA Kit is designed for the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of plant species. (omegabiotek.com)
  • As plants grew and thrived across the plains, valleys and mountains of Earth's landscape, rapid changes in their structures gave rise to a myriad of new species, and the group's data also helps scientists better understand the ancestry of the most common plant lineages, including flowering plants and nonflowering cone-bearing plants such pine trees. (umich.edu)
  • The investigation has also revealed a number of previously unknown molecular characteristics of some plant species that may have applications in medicine and industry. (umich.edu)
  • On the basis of nuclear ( ITS ), plastid ( rbc LS) and mitochondrial ( COI ) DNA sequence data, we have analyzed the genetic composition of typical Saccharina japonica (TYP) and its two common morphological varieties, known as the "longipes" (LON) and "shallow-water" (SHA) forms seeking to clarify their taxonomical status and to evaluate the possibility of cryptic species within S. japonica . (biomedcentral.com)
  • DNA barcoding is impending towards the generation of universal standards for species discrimination with a standard gene region that can be sequenced accurately and within short span of time. (biomedcentral.com)
  • Concerning gene targeting in rice, an important staple food and a model plant for other cereal species, we have developed a large-scale Agrobacterium-mediated transformation procedure with a strong positive-negative selection and succeeded a reproducible targeting of the Waxy gene by homologous recombination without concomitant occurrence of undesirable ectopic events. (nii.ac.jp)
  • Working with a sub-contract from the Defense Logistics Agency, researchers at Applied DNA Sciences Inc. have figured out how to create unique DNA 'signatures' out of plant genomes. (wired.com)
  • We still have a big risk now because there are so few plant mitochondrial genomes used in the world. (eurekalert.org)
  • Plant mitochondrial genomes are a different story. (eurekalert.org)
  • A study appearing online this week in the Proceedings of the National Academy of Sciences unravels 100 million years of evolution through an extensive analysis of plant genomes. (innovations-report.com)
  • The DNA contained within Paris japonica dwarves all other plant and animal genomes that have been analysed so far. (wn.com)
  • This study provides new insights into the structural impact of inserting foreign fragments into plant genomes and demonstrates the utility of state-of-the-art long-range sequencing technologies to rapidly identify unanticipated genomic changes. (jcvi.org)
  • Turmel M, Otis C, Lemieux C. The complete chloroplast DNA sequence of the green alga Nephroselmis olivacea: insights into the architecture of ancestral chloroplast genomes. (umassmed.edu)
  • In contrast to the DNA contained in animal and fungal mitochondria, plant mitochondrial genomes have significantly larger amounts of DNA, but not significantly more genes. (unl.edu)
  • Imelfort M and Edwards D (2009) De novo sequencing of plant genomes using second-generation technologies. (springer.com)
  • Generally fresh leaves aged 15-20 days are preferred for plant tissues (fresh, freeze-dried, or frozen in liquid nitrogen) and usually ruptured by mechanical force in pestle and motor or TissueLyser. (intechopen.com)
  • The Direct PCR approach facilitates PCR amplification directly from small amounts of unpurified samples, and is demonstrated here for several plant and animal tissues ( Figure 1 ). (jove.com)
  • Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. (bioline.com)
  • Fast purification of high-quality genomic DNA from a variety of plant tissues, including leaves, bark, roots and fruits. (bioline.com)
  • The Genespin™ semi-automated high-throughput DNA extraction instrument utilises spin plates and Kleargene spin-purification chemistry for high-throughput DNA extraction from plant tissues in 96- and 384-well plates. (biosearchtech.com)
  • The detection and quantification of nucleosides through enzymatic hydrolyses notably increases the specificity of the technique and allows its exploitation in the analysis of poorly purified and/or concentrated DNA samples such as those obtained from meristematic plant regions and paraffin-embedded tissues. (nih.gov)
  • For total RNA isolation from 50-500 mg of any type of plant and animal tissues or cultured cells. (mpbio.com)
  • Sharma R., Damgaard D., Alexander T.W., Dugan M.E.R., Aalhus J.L., Stanford K., McAllister T.A. (2006) Detection of transgenic and endogenous plant DNA in tissues of sheep and pigs fed Roundup Ready canola meal. (testbiotech.org)
  • Total DNA, including genomic DNA, mitochondrial DNA and chloroplast DNA can be purified from fresh or frozen plant tissues, plant cells or fungi samples using this kit. (norgenbiotek.com)
  • The Mag-Bind® Plant DNA DS 96 Kit allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content. (omegabiotek.com)
  • Allprotect Tissue Reagent is optimized to protect RNA, DNA, and proteins in humans and animal tissues. (qiagen.com)
  • However, Allprotect can also be used with plant tissues. (qiagen.com)
  • The database should provide DNA barcodes for data retrieval and similarity search. (nih.gov)
  • In order to obtain such barcodes, several molecular methods have been applied to develop markers that aid with the authentication and identification of medicinal plant materials. (nih.gov)
  • Our objective in this paper is not to debate the validity of using barcodes for plant identification, but rather to determine appropriate DNA regions for use in flowering plants. (pnas.org)
  • DNA Barcodes: Methods and Protocols. (si.edu)
  • Generating Plant DNA Barcodes for Trees in Long-Term Forest Dynamics Plots. (si.edu)
  • Advances in the Use of DNA Barcodes to Build a Community Phylogeny for Tropical Trees in a Puerto Rican Forest Dynamics Plot. (si.edu)
  • DNA barcodes: Genes, genomics, and bioinformatics. (si.edu)
  • Our results clearly suggest that, for most angiosperm groups, plastid markers will not be the most appropriate for use as DNA barcodes. (wiley.com)
  • We therefore advocate shifting the focus from plastid to nuclear markers to achieve an overall higher success using DNA barcodes. (wiley.com)
  • In the future we'd like to see this idea of reading plants' genetic barcodes translated into a portable device that can be taken into any environment, which can quickly and easily analyse any plant sample's matK DNA and compare it to a vast database of information, allowing almost instantaneous identification, " he says. (bio-medicine.org)
  • Natural barcodes (a short strand of deoxyribonucleic acid (DNA) (the genetic code unique to each living organism and some viruses) that consists of between 300-800 base pairs (bps) - Adenine (A)-Thymidine (T), and Cytosine (C)-Guanine (G)) that can be represented by different colors) also exist and are well established in the animal kingdom. (colorbasepair.com)
  • DNA extraction and amplification") as reported by W. John Kress and David L. Erickson, DNA barcodes: Genes, genomics, and bioinformatics (PNAS. (colorbasepair.com)
  • Hebert PD, Cywinska A, Ball SL, et al (2003) Biological identifications through DNA barcodes. (springer.com)
  • Nuclear DNA was first edited in the early 1970s, chloroplast DNA was first edited in 1988, and animal mitochondrial DNA was edited in 2008. (eurekalert.org)
  • 4. Chloroplast DNA and Phylogenetic Relationships. (crcpress.com)
  • The procedure yields total cellular DNA (i.e. nuclear, chloroplast, and mitochondrial DNA). (nih.gov)
  • This view prevailed until 2008 when a team led by Dr. Vincent Savolainen of Imperial College London's Department of Life Sciences and The Royal Botanic Gardens, Kew, studied the functionality of the megakaryocyte-associated tyrosine-protein kinase (matK) gene located in the intron of trnK chloroplast genes found in plant leaves. (colorbasepair.com)
  • This kit is designed for manual or fully automated high throughput preparation of genomic, chloroplast, and mitochondrial DNA. (omegabiotek.com)
  • Nuclear DNA is the long double-helix genetic material inherited from both parents. (eurekalert.org)
  • Even the basic "lab rat" of plant science, Arabidopsis , has a genetic code that's about 1.5 times as long as U. gibba's. (latimes.com)
  • And yet the plant packs efficiently, stuffing all its useful genetic code into a fraction of the sprawling DNA real estate afforded other plants and animals. (latimes.com)
  • To make the DNA tags scientists extract genetic code from plants and reassemble it to be distinct from any other DNA on the planet. (scientificamerican.com)
  • Key experiments in the control of plant transgene expression were performed by Jorgensen after he joined DNA Plant Technology corporation / Advanced Genetic Sciences, Inc., including the modification of flower color in ornamental plants. (wikipedia.org)
  • Scientists have known for some time that a master gene named SOG1 acts like a general contractor for repair, coordinating with various genetic subcontractors of the plant cell to mount an effective DNA damage response. (eurekalert.org)
  • The research, which appeared in the journal Proceedings of the National Academy of Sciences during the week of October 10, 2018, reveals the genetic framework controlling a complex biological process that has broad implications for understanding how plants in particular, and organisms in general, cope with DNA damage to ensure long-term health and fitness. (eurekalert.org)
  • Mustard weed may be an annoyance for farmers, but a group of University researchers hope to use the plant to unravel genetic mysteries. (mndaily.com)
  • Henry Daniell, a professor in the departments of Biochemistry and Pathology in the University of Pennsylvania's School of Dental Medicine, has found great success in using genetic engineering to coax lettuce and tobacco plants to produce foreign proteins in their leaves, be they from a polio virus to make vaccines, a wormwood plant to synthesize malaria drugs, or the human clotting factor to make a hemophilia treatment. (phys.org)
  • Little chance that genetic modifications to chloroplasts are transferred by pollen to wild plants. (dnalc.org)
  • We're going to be using a new UK plant DNA barcode library , as well as environmental genomic technologies to identify complex mixtures of tree and grass pollens from a molecular genetic perspective. (bangor.ac.uk)
  • Genetic and biochemical evidence indicate that at many repeat loci, DDB2 influences de novo DNA methylation by interacting with ARGONAUTE4 and by controlling the local abundance of 24-nucleotide short interfering RNAs (siRNAs). (plantcell.org)
  • Deoxyribonucleic acid that makes up the genetic material of plants. (umassmed.edu)
  • The findings, which will be published on June 18 in BMC Biology , highlight the power of an evolutionary process called tandem gene duplication, in which accidental copying of DNA DNA, or deoxyribonucleic acid, is a molecule composed of two long strands of nucleotides that coil around each other to form a double helix. It is the hereditary material in humans and almost all other organisms that carries genetic instructions for development, functioning, growth, and reproduction. Nearly every cell in a person's body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA). ">DNA gives organisms flexibility to expand the arsenal of genetic tools they have at their disposal. (scitechdaily.com)
  • Genetic engineering becomes increasingly important as a tool in plant breeding. (ejbiotechnology.info)
  • Plant bZIP proteins exhibit a relaxed DNA-binding specificity for DNA sequence motifs containing an ACGT core. (nih.gov)
  • The Role of the Computer in Estimates of DNA Nucleotide Sequence Divergence. (crcpress.com)
  • The KN1 DNA-binding sequence, TGACAG(G/C)T, was biochemically identified, and in vitro DNA-binding assays show that individually KN1 and the HD of KIP bind specifically to this motif with low affinity. (pnas.org)
  • The HD is a conserved structure that contains three α helices, and a sequence motif in the third helix recognizes and binds to the appropriate DNA sequence ( 15 ). (pnas.org)
  • T he identification of animal biological diversity by using molecular markers has recently been proposed and demonstrated on a large scale through the use of a short DNA sequence in the cytochrome c oxidase 1 (CO1) gene ( 1 - 5 ). (pnas.org)
  • The technique enables them to detect and sequence the short lengths of damaged DNA that are cut from chromosomes during the excision repair process. (eurekalert.org)
  • The inversions create major alterations to the sequence of the transcribed RNA with relatively minor changes to the DNA structure. (frontiersin.org)
  • Using their results from this analysis, they designed a software program that converts any given DNA sequence into the sequence that would be preferred by either lettuce or tobacco plants . (phys.org)
  • A Male-Associated DNA Sequence in a Dioecious Plant, Cannabis sativa L. (nii.ac.jp)
  • The 730-bp DNA fragment was named MADCl (male-associated DNA sequence in Cannabis sativa). (nii.ac.jp)
  • Smith LM, Sanders JZ, Kaiser RJ, et al (1986) Fluorescence detection in automated DNA sequence analysis. (springer.com)
  • All ten plant bZIP proteins examined, except TGA1a, exhibited type A G-box binding activity preferring class I G-box elements. (nih.gov)
  • Information provided by our systematic analysis of plant bZIP DNA binding specificity can be used to identify high affinity binding sites for the plant bZIP proteins studied here. (nih.gov)
  • The rest of it is non-coding DNA that doesn't appear to carry active, relevant information for that living creature's proper functioning (i.e. for building proteins). (latimes.com)
  • The knox gene family was the first group of plant genes identified that encodes homeodomain (HD) proteins ( 14 ). (pnas.org)
  • Maintaining yield and quality of DNA during plant DNA extraction is one of the difficult tasks compared to that of animals, because of its rigid cell wall, which is made up of cellulose along with other variable levels of chemical components such as polysaccharides, polyphenols, proteins, and lipids that act as a contaminant during DNA extraction. (intechopen.com)
  • The basic principle of DNA isolation is disruption of the cell wall, cell membrane, and nuclear membrane to release the highly intact DNA into solution followed by precipitation of DNA and removal of the contaminating biomolecules such as the proteins, polysaccharides, lipids, phenols, and other secondary metabolites by enzymatic or chemical methods [ 4 ]. (intechopen.com)
  • One strand of DNA is used to create RNA, which is in turn used to create proteins. (mndaily.com)
  • This 3D animation shows how proteins are made in the cell from the information in the DNA code. (wn.com)
  • One explanation for this, Daniell and colleagues hypothesized, could have to do with differences among plants, animals, bacteria, and viruses in how they use the DNA code to make proteins. (phys.org)
  • Proteins are made up of building blocks called amino acids , which are themselves produced according to three-letter strings of DNA called codons. (phys.org)
  • DNA methylation is often low when TFs bind and several TF known to bind enhancers are actually DNA methylation sensitive proteins! (protocol-online.org)
  • The reason the strategy can be successful is that the plant has evolved to recognize the presence of only certain bacterial proteins as warnings of an infection. (bio-medicine.org)
  • If some bacterial proteins give away the presence of the pathogen to the plant, why are they and their surrounding genomic islands maintained by the bacteria at all? (bio-medicine.org)
  • DNA damage detection and repair take place in the context of chromatin, and histone proteins play important roles in these events. (biochemj.org)
  • Post-translational modifications of histone proteins are involved in repair and DNA damage signalling processes in response to genotoxic stresses. (biochemj.org)
  • Can Allprotect Tissue Reagent be used to stabilize RNA, DNA and proteins in cells, bacteria, yeast, and plants? (qiagen.com)
  • Taken together, these studies suggest that RGBPs are new types of telomere-binding proteins that bind in vitro to single-stranded G-rich telomere DNA in the angiosperms. (deepdyve.com)
  • Telomeres are specific protein-DNA complexes that protect the ends of eukaryotic chromosomes from fusion and degradation and are maintained by a specialized mechanism exerted by telomerase and telomere-binding proteins (TBPs), which are evolutionarily conserved. (ovid.com)
  • DNA-binding studies with mutant AtTBP1 proteins showed that the Myb-extension domain of AtTBP1 is required for binding to plant telomeric DNA. (ovid.com)
  • 8. A Critical Review of Some Terminologies Used for Additional DNA in Plant Chromosomes. (crcpress.com)
  • Telomerase is the enzyme that creates the DNA of telomeres, the compound structures located at the tips of our chromosomes. (katc.com)
  • They extracted telomere DNA from a single-celled organism in pond scum, showed how it protected chromosomes in yeast, and identified and named the enzyme telomerase that builds the DNA of telomeres and extends their lives. (katc.com)
  • The secret, they say, is to 'paint' the plant chromosomes before trying to understand their origins. (newscientist.com)
  • It involves persuading the DNA in plant chromosomes to 'mop up' fluorescent 'probes' - molecules themselves made of DNA - that can act as a guide to the ancestry and character of the chromosomes. (newscientist.com)
  • The role of repetitive DNA in structure and evolution of sex chromosomes in plants. (biomedsearch.com)
  • Only 3% of this aquatic plant's DNA is not part of a known gene , new research shows. (slashdot.org)
  • In contrast, only 2% of human DNA is part of a gene. (slashdot.org)
  • Corn with that specific mitochondrial gene has not been planted since. (eurekalert.org)
  • 3. A recombinant DNA molecule according to claim 2 , wherein the said expressible DNA comprises a structural gene. (google.com)
  • 4. A recombinant DNA molecule according to claim 3 , wherein the said structural gene upon expression leads to resistance against plant pathogens selected from the group consisting of insects, fungi, bacteria and viruses. (google.com)
  • 5. A recombinant DNA molecule according to claim 4 , wherein the said structural gene codes for a polypeptide that is toxic to insects and/or their larvae. (google.com)
  • 7. A recombinant DNA molecule according to claim 6 , wherein the said crystalline protein is encoded by a synthetic B.t. gene. (google.com)
  • 8. A recombinant DNA molecule according to claim 3 , wherein the said structural gene codes for a lytic peptide. (google.com)
  • A portion of the mitochondrial CO1 gene was deliberately chosen for use in animal identification when DNA barcoding was proposed ( 1 ), and its broad utility in animal systems has been demonstrated in subsequent pilot studies ( 1 - 5 ). (pnas.org)
  • Early/late gene expression (transcription) and the DNA replication stages can occur either in the cytoplasm (poxviruses) or in the nucleus of the infected cell. (els.net)
  • While working for DNA Plant Technology, the scientists Richard A. Jorgensen and Carolyn Napoli made discoveries about post transcriptional gene silencing that went on to form the basis of a number of U.S. patents on gene regulation and crop manipulation. (wikipedia.org)
  • To better understand the dynamics of gene regulation throughout the DNA damage response and to determine the direct roles of SOG1 in this response, Law and her team conducted a series of experiments in Arabidopsis thaliana , a weed commonly used for genetics research. (eurekalert.org)
  • It's exciting to get more clarity on the specific gene networks and subnetworks involved in the DNA damage response, as well as their timing, which had not been done before," says Law. (eurekalert.org)
  • A method is provided for introducing a foreign gene into a plant cell by means of an Olpidium zoospore vector having associated with it a reassembled nucleoprotein complex comprising the foreign gene and reassociated coat protein of a zoospore-transmissable virus. (google.com)
  • Powerful new techniques for gene transfer recently have been developed for moving single genes and whole blocks of genes from one plant to another and even for moving genes from non-plants into plants. (google.com)
  • These new techniques are useful for generating specific plant genotypes and for the long term achievement of greater plant diversity through gene recombination. (google.com)
  • A recurring difficulty when interpreting these protein-DNA interactions is that they poorly relate to activity of the associated gene. (plantcell.org)
  • That is, when a bona fide protein-DNA interaction is disrupted, often the activity of the associated gene does not change. (plantcell.org)
  • The gene gun worked with a broader range of plant crops than agrobacterium in the early days and so it was a more random method, it was also more broadly useful. (dnalc.org)
  • Robert Horsch talks about the gene gun: a physical method of delivering genes into plant cells. (dnalc.org)
  • In contrast, SAIL_232 line T-DNA insertions are predominantly targeted by 21/22nt siRNAs, with DNA methylation and silencing limited to a reporter, but not the resistance gene. (jcvi.org)
  • Complementary DNA has been isolated that codes for maize nitrite reductase (NiR) by using the corresponding spinach gene (E Back et al . (plantphysiol.org)
  • DNA 'barcode' identified for plants ( A 'barcode' gene that can be used to. (bio-medicine.org)
  • Genomic DNA was isolated using ISOLATE II Plant DNA Kit and a 1.4 kb fragment of AOS gene was amplified from the isolated DNA. (bioline.com)
  • Genomic DNA was isolated from 20 mg freeze-dried budding leaves of A. thaliana using ISOLATE II Plant DNA Kit and a 2-fold serial dilution of the isolated DNA (200 ng to 6.25 ng, lanes 1-6 respectively) was used to amplify a 1.4 kb fragment of AOS gene. (bioline.com)
  • It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. (norgenbiotek.com)
  • Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results. (norgenbiotek.com)
  • GT-1, a DNA-binding protein that interacts with BoxII in vitro, is a good candidate for being a light-modulated molecular switch controlling gene expression. (deepdyve.com)
  • In order to assess the uniformity and the stability of transfer DNA (T-DNA) integration and gene expression, we studied 26 transgenic apple lines carrying the attacin E gene from Hyalophora cecropia , the β-glucuronidase gene, and the nptII gene. (ejbiotechnology.info)
  • The choice of a DNA region usable for barcoding has been little investigated in other eukaryotes, whereas in prokaryotes, rRNA genes are favored for identifications (e.g., ref. 15 ). (pnas.org)
  • It revealed that the "nucleotide excision repair" system works much more efficiently in the active genes of plants as compared to humans. (eurekalert.org)
  • But, it wasn't clear which specific genes were among the subcontractors, nor how SOG1 interacted with them to oversee the DNA damage response. (eurekalert.org)
  • They found the expression of approximately 2,400 genes went up or down in response to DNA damage during that time period, almost all of which depended on SOG1's presence. (eurekalert.org)
  • This resulted in the identification of 11 groups of genes that act on different time scales and play known or predicted roles in different aspects of the plants' response to DNA damage. (eurekalert.org)
  • Next, the lab plans to study the roles of new factors implicated in the DNA damage response based on their expression profiles and to continue exploring the network of genes being directly or indirectly controlled by SOG1. (eurekalert.org)
  • This invention relates to a novel method for introducing foreign genes into both monocotyledonous and dicotyledonous plants, thereby circumventing many of the limitations associated with the present-day technology in this field. (google.com)
  • Yet over the years, he noticed that while his plant-based drug production platform could efficiently express bacterial genes as well as short human genes, it had trouble expressing viral genes and longer human genes. (phys.org)
  • Analysis of genomic DNA supports the notion that there are at least two NiR genes in maize. (plantphysiol.org)
  • The identification of key genes controlling these processes, the expression of which is altered in plants with low methylation, opens the way to understanding how DNA methylation regulates plant development. (deepdyve.com)
  • The mutable flecked, speckled, r-1, and purple alleles in I. nil were caused by insertions of Tpn1 and its relatives in the En/Spm superfamily, Tpn2, Tpn3, and Tpn4, into the genes for anthocyanin coloration in wers, i.e. (nii.ac.jp)
  • By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples. (bioline.com)
  • Our portfolio of high-performance DNA and RNA extraction and purifications kits, automated DNA purification instruments, and tailor-made extraction services deliver high-quality DNA and RNA for downstream sequencing and PCR applications enabling you to make critical research decisions and advance traits to market. (biosearchtech.com)
  • The oKtopure™ automated DNA extraction and purification system is a platform which combines standardised high-throughput DNA extraction from plant tissue with our proprietary sbeadex™ magnetic bead based extraction chemistry. (biosearchtech.com)
  • Pairing the oKtopure system with sbeadex chemistry provides an industry-leading solution driving laboratory efficiency and obtaining high quality and high-throughput DNA extraction and purification to feed downstream NGS, microarray, and PCR workflows. (biosearchtech.com)
  • The MasterPure Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types. (biosearchtech.com)
  • Plant RNA/DNA Purification Kit (Cat. (norgenbiotek.com)
  • This kit provides for rapid spin column isolation and purification of total RNA and genomic DNA simultaneously from a single plant sample without splitting the lyate. (norgenbiotek.com)
  • Norgen Biotek is an innovative biotechnology company providing RNA, DNA and protein purification kits of exceptional quality, ease-of-use and sensitivity. (norgenbiotek.com)
  • Norgen Biotek Corp. is dedicated to providing our customers with first class sample preparation kits for RNA, microRNA, DNA and protein purification, clean-up and concentration and to provide dedicated and expert support services to our customers and partners worldwide. (norgenbiotek.com)
  • Further comprised by the invention are recombinant DNA, plasmid and vector molecules suitably adapted to the specific conditions of the process according to the invention and the transgenic plant products obtainable in accordance with the said process. (google.com)
  • Nature 295:257-259 (1982)] and calcium phosphate coprecipitation with DNA or recombinant bacteriophage [F. L. Graham et al. (google.com)
  • Can glutamine synthetase activity levels be modulated in transgenic plants by the use of recombinant DNA technology? (portlandpress.com)
  • We therefore propose the nuclear internal transcribed spacer region and the plastid trnH-psbA intergenic spacer as potentially usable DNA regions for applying barcoding to flowering plants. (pnas.org)
  • 1976. Nuclear DNA amounts in angiosperms. (wikipedia.org)
  • 1997. Nuclear DNA amounts in angiosperms - 583 new estimates. (wikipedia.org)
  • 2000. Nuclear DNA amounts in angiosperms and their modern uses-807 new estimates. (wikipedia.org)
  • 2005. Nuclear DNA amounts in angiosperms - progress, problems and prospects. (wikipedia.org)
  • Nuclear DNA Amounts in Gymnosperms. (wikipedia.org)
  • Three types of specific DNA-protein complexes (I, II, and III) were identified by gel retardation assays using synthetic telomere substrates consisting of two or more single-stranded TTTAGGG repeats and rice nuclear extracts. (deepdyve.com)
  • We further showed that nuclear GT-1 DNA-binding activity to BoxII was reduced by treatment with calf intestine phosphatase in extracts prepared from light-grown plants but not from etiolated plants. (deepdyve.com)
  • Most of the plant DNA isolation protocols used today are modified versions of hexadecyltrimethyl-ammonium bromide (CTAB) extraction procedure. (intechopen.com)
  • CTAB, NaCl, PVP, ethanol, and isopropanol) used during the DNA extraction procedure will benefit to set or modify protocols for more precisions. (intechopen.com)
  • A review of the chemicals used in the CTAB method of DNA extraction and their probable functions on the highly evolved yet complex to students and researchers has been summarized. (intechopen.com)
  • These contaminants can be removed during extraction by standardizing basic DNA extraction protocol [ 1 , 2 , 3 ]. (intechopen.com)
  • Here we have used a special extraction buffer which is applicable for every plant. (wn.com)
  • The results illustrating that the ISOLATE II Plant DNA Kit gives consistent results with different extraction techniques. (bioline.com)
  • Achieve greater flexibility and efficiency with our DNA and RNA extraction services. (biosearchtech.com)
  • The QuickExtract™ Plant DNA Extraction Solution provides a rapid, inexpensive method for isolating PCR-ready DNA from plant tissue and other sample types, making this kit ideal for processing hundreds of samples simultaneously. (biosearchtech.com)
  • The oKtopure instrument uses the proprietary sbeadex magnetic bead based chemistry to perform automated DNA extraction from 8 x 96-well plates in parallel. (biosearchtech.com)
  • Burrows, P.R., and Boffey, S.A., 1986, A technique for the extraction and restriction endonuclease digestion of total DNA from Globodera rostochiensis and G. pallida second stage juveniles, Revue Nematol . (springer.com)
  • The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes (Fig. 1). (lucigen.com)
  • Overview of the QuickExtract™ Plant DNA extraction procedure. (lucigen.com)
  • PCR products using QuickExtract™ Plant DNA Extraction Solution with different varieties of plant leaves. (lucigen.com)
  • Reproducibility of PCR results with QuickExtract™ Plant DNA Extraction Solution from Arabidopsis thaliana leaves. (lucigen.com)
  • Purified DNA is ready for downstream applications such as PCR (Figure 2), restriction endonuclease digestions, cloning and southern blots. (sigmaaldrich.com)
  • We carefully verified the identity of Goji reference plant material based on morphological traits, mainly floral structures of several inflorescences of each individual, in order to create a robust background for the downstream applications that were used on those reference plants and additionally on commercial Goji products. (frontiersin.org)
  • The results illustrating that the ISOLATE II Plant DNA Kit gives high yields of high quality DNA, ideal for all downstream applications. (bioline.com)
  • Isolating DNA from soil samples is a big challenge because samples are full of inhibitors like polymers or low-molecular substances, which inhibit downstream applications. (bioline.com)
  • The instrument delivers high quality DNA suitable for downstream applications such as sequencing, microarrays and SNP genotyping. (biosearchtech.com)
  • The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, SNP, Southern blotting and sequencing. (norgenbiotek.com)
  • These libraries are to then be supplied to researchers and the DNA preserved for thousands of years. (bio.net)
  • Researchers in Japan have edited plant mitochondrial DNA for the first time, which could lead to a more secure food supply. (eurekalert.org)
  • Researchers refer to a common type of plant male infertility as cytoplasmic male sterility (CMS). (eurekalert.org)
  • But the carnivorous bladderwort plant, Utricularia gibba , has only about 3% junk, according to an international team of researchers -- which is unusual even by plant standards. (latimes.com)
  • Clearly the plant must be cutting unnecessary DNA faster than it's adding it, the researchers concluded. (latimes.com)
  • The UNC researchers performed XR-seq scans on cells from UV-exposed plants - Arabidopsis thaliana, the "lab rat" of plant research also known as thale cress or mouse-ear cress. (eurekalert.org)
  • The origins of flowering plants from peas to oak trees are now in clearer focus thanks to the efforts of University of Florida researchers. (innovations-report.com)
  • But continual improvements in DNA sequencing technology are now allowing researchers to analyze those larger amounts of data more quickly. (innovations-report.com)
  • Researchers at the National Institute of Standards and Technology (NIST) and the University of Massachusetts Amherst (UMass) have provided the first evidence that engineered nanoparticles are able to accumulate within plants and damage their DNA. (innovations-report.com)
  • If the former, the researchers also wanted to find out if nanosizing had any substantial effects on plant growth and health. (innovations-report.com)
  • The researchers made this discovery by analysing the DNA from differen. (bio-medicine.org)
  • In order to understand how Chinese plants were related to each other, UF researchers and Chinese scientists analyzed plant DNA to map family trees. (alligator.org)
  • UF researchers Pam Soltis, her husband, Doug Soltis and Miao Sun identified areas in East China with high level of plant diversity, but existing nature preserves to protect plants are primarily in the West. (alligator.org)
  • The National Science Foundation gave researchers a $1,199,043 grant to fund their Chinese plant study, Doug Soltis said. (alligator.org)
  • My involvement with this fabulous group of researchers has allowed me to accelerate my own research on weedy plant genomics while at the same time contribute to a broader study on the phylogenetics of land plant evolution. (umich.edu)
  • A recent Testbiotech survey shows that DNA fragments from transgenic plants are increasingly found in animal tissue such as milk, inner organs and muscles. (testbiotech.org)
  • These findings are not the first to be reported after DNA fragments have been found in the tissue of animals fed with transgenic plants. (testbiotech.org)
  • More recently, research found traces from transgenic plants in the organs of fish, namely rainbow trout and tilapia. (testbiotech.org)
  • Using a comprehensive DNA barcode library to detect novel egg and larval host plant associations in a Cephaloleia rolled-leaf beetle (Coleoptera: Chrysomelidae). (si.edu)
  • 2011. Exploring Tree-Habitat Associations in a Chinese Subtropical Forest Plot Using a Molecular Phylogeny Generated from DNA Barcode Loci. (si.edu)
  • 2009. A DNA barcode for land plants . (si.edu)
  • Chase MW, Cowan RS, Hollingsworth PM, et al (2007) A proposal for a standardised protocol to barcode all land plants. (springer.com)
  • Hollingsworth P, Forrest L, Spouge J, et al (2009) A DNA barcode for land plants. (springer.com)
  • However, it endures complications in amplification that render it feasibility as a universal barcode for land plants. (biomedcentral.com)
  • 2. The method of claim 1 wherein said nucleic acid is plasmid DNA. (google.com)
  • 6. The vector of claim 5 wherein said nucleic acid is plasmid DNA. (google.com)
  • Several methods have been explored for introducing nucleic acid into a variety of cell types with the hope of developing a successful technique for the transformation of eukaryotic cells of plants and animals. (google.com)
  • DNA concentration determined via fluorescence-based nucleic acid quantification. (omegabiotek.com)
  • DNA barcoding of medicinal plant material for identification. (nih.gov)
  • Here, we propose two DNA regions for barcoding plants and provide an initial test of their utility. (pnas.org)
  • 1 - 4 ), and Kress ( 14 ) have offered arguments for the utility of DNA barcoding as a powerful framework for identifying specimens. (pnas.org)
  • Plant DNA barcoding research is shifting beyond performance comparisons of different DNA regions towards practical applications. (slideshare.net)
  • In plants, establishing a standardized DNA barcoding system has been more challenging. (slideshare.net)
  • 2012. DNA Barcoding as a Reliable Method for the Authentication of Commercial Seafood Products. (si.edu)
  • 2008. DNA barcoding in land plants: developing standards to quantify and maximize success. (si.edu)
  • DNA barcoding - a windfall for tropical biology? (si.edu)
  • Is the Subject Area "DNA barcoding" applicable to this article? (plos.org)
  • We present an overview of recent publications that demonstrate the use of "NGS" technology for DNA fingerprinting and DNA barcoding applications. (springer.com)
  • We present new data for numerous Schisandraceae, the largest ANITA family, from fieldwork, nocturnal filming, electron microscopy, barcoding and molecular clocks to infer pollinator/plant interactions over multiple years at sites throughout China to test the extent of pollinator specificity. (figshare.com)
  • This study provides a report on DNA barcoding for unique insectivores' Nepenthes genus. (biomedcentral.com)
  • The trnH-psbA spacer, although short (≈450-bp), is the most variable plastid region in angiosperms and is easily amplified across a broad range of land plants. (pnas.org)
  • The present invention relates to a novel method of inserting viral DNA, which optionally may contain cargo-DNA, into plants or viable parts thereof, but preferably into plants of the monocotyledon class, and most preferably into plants of the family Gramineae, using suitable transfer microorganisms. (google.com)
  • In the case of plant DNA viruses, there is a cell wall that imposes restrictions on viral release. (els.net)
  • In this case, it is also important that intercellular movement of viral particles is achieved through the plasmodesmata, unique structures that allow communication between the cytoplasm of neighbouring plant cells. (els.net)
  • In addition to the standard scanning process to start translation, two other mechanisms (ribosome shunting and leaky scanning) are used by different DNA viruses to control translation of various viral ORFs. (els.net)
  • Purified DNA samples can be used for the detection of viral pathogens, as viral DNA is isolated with the plant/fungi DNA. (norgenbiotek.com)
  • NucleoSpin Plant II is a next-generation kit designed for the rapid isolation of genomic DNA from plant cells and tissue, with higher yield and quality. (clontech.com)
  • The unnamed luxury goods company has enlisted the services of Applied DNA Sciences, based in Stony Brook, New York. (popsci.com)
  • Now a biotech company called Applied DNA Sciences may have an original solution: tagging legitimate drugs with engineered DNA. (scientificamerican.com)
  • If you consider the morbidity and mortality caused by the scourge of counterfeit drugs, it is an urgent problem that requires a global response," says Jim Hayward , the CEO of Applied DNA Sciences. (scientificamerican.com)
  • Applied DNA Sciences calls its technique SigNature DNA, which could be used in myriad products in addition to pharmaceuticals. (scientificamerican.com)
  • Albert, professor of biological sciences in the UB College of Arts and Sciences and a visiting professor at Nanyang Technological University in Singapore, and his students made important contributions, conducting bioinformatics research that helped unravel the evolutionary history of crocin and caffeine synthesis in the gardenia and coffee plants, respectively. (scitechdaily.com)
  • A comment on one of my earlier articles in March, Counterfeiting: The Battle Is On, asked me to look into Applied DNA Sciences (APDN) and its botanical DNA applications. (technologynetworks.com)
  • Fortunately, I was able to contact a key scientist with significant DNA knowledge: Vicky Markstein, president of Life Sciences Society and IEEE CS Bioinformatics General Chair. (technologynetworks.com)
  • The kit contains all the reagents, columns and tubes necessary to isolate genomic DNA from up to 100 mg of fresh or 20 mg of freeze-dried plant tissue. (sigmaaldrich.com)
  • Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. (sigmaaldrich.com)
  • DNA Plant Technology was founded in 1981 by Dr. William R. Sharp and Dr. David A. Evans, in Cinnaminison, New Jersey, "to develop tastier, value-added plant-based products for industrial and consumer markets" using "advanced plant-breeding techniques, tissue-culture methods and molecular biology in developing premium food products and improving agricultural raw materials. (wikipedia.org)
  • As long as you handle the plant tissue properly, there should be no difference between your fresh and dried samples. (protocol-online.org)
  • This will minimize DNase activity in the plant tissue before you extract the DNA. (protocol-online.org)
  • 20 mg plant tissue (dry weight) with yields typically ranging from 1-30 µg genomic DNA. (clontech.com)
  • The NucleoSpin Plant Midi and Plant Maxi Kits are designed for isolating genomic DNA from medium to large samples of plant tissue. (clontech.com)
  • 1977. Polyphenols of mature plant, seedling and tissue cultures of Theobroma cacao . (springer.com)
  • The Direct PCR approach presented here facilitates PCR amplification directly from small amounts of unpurified plant and animal tissue. (jove.com)
  • Two new ready-to-use reagent kits offer oxidative protection when working with sensitive plant tissue such as maize, sugar beet, rice and bananas. (selectscience.net)
  • While oxidation in its classic literal sense is the chemical reaction of a substance with oxygen, the endogenous oxidants of certain plants can induce a browning reaction of the tissue sample. (selectscience.net)
  • Our kits come with a complete set of tools for collecting plant and animal tissue samples in an automation-friendly format. (biosearchtech.com)
  • The system combines CTAB-based lysis, which eliminates the need for organic solvents, with the convenience of Mag-Bind® Particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. (omegabiotek.com)
  • If the plants leaves are waxy, the surface of the leaves can be scratched to allow Allprotect reagent to permeate the tissue. (qiagen.com)
  • Here we found that the jump in efficiency for transcription-coupled repair is even more pronounced in plants than it is in animals or bacteria," Oztas said. (eurekalert.org)
  • World 's Oldest Known DNA Discovered http://news.discovery.com/earth/weather-extreme-events/oldest-dna-bacteria-discovered .htm It won't make Jurassic Park a reality, but scientists have discovered 419 million-year-old DNA intact inside ancient salt deposits. (wn.com)
  • Remarkably, bacteria that have excised the genomic island do not appear compromised in their ability to grow and cause disease within the host bean plant. (bio-medicine.org)
  • Plants get a significant portion of their energy through the same "powerhouse of the cell" that produces energy in animal cells: the mitochondria. (eurekalert.org)
  • In plants, development of the aerial shoot is controlled by a group of cells in the shoot apex called the shoot apical meristem (SAM). (pnas.org)
  • DNA viruses have evolved very different replication strategies as well as a rich variety of molecular interactions with their host cells. (els.net)
  • DePamphilis ML (1996) DNA Replication in Eukaryotic Cells. (els.net)
  • Just as a building with structural damage can be unsafe, cells with DNA damage that goes unnoticed or unrepaired can be dangerous," says Assistant Professor Julie Law, the senior author of the paper. (eurekalert.org)
  • In a recent paper,* the team led by NIST chemist Bryant C. Nelson showed that under laboratory conditions, cupric oxide nanoparticles have the capacity to enter plant root cells and generate many mutagenic DNA base lesions. (innovations-report.com)
  • This indicates that these compounds have important roles in the regulation of metabolism in plant cells, especially in connection to stress. (diva-portal.org)
  • Mitochondria, a component of cells that have their own DNA (mtDNA), produce energy for the body, among other functions. (pennmedicine.org)
  • This two-step transdermal delivery treatment contains groundbreaking Hyaluronic Acid Micro-Pyramids that deliver a rejuvenating cocktail of Hyaluronic Acid, Plant-EGF, Plant Stem Cells and Floret DNA to directly transform the skin, focus on repair and reverse signs of aging. (skinstore.com)
  • Characterization of protein-protein and protein-DNA interactions is critical to understand mechanisms governing the biology of cells. (muni.cz)
  • These techniques are based on the usage of a mixture of leaf cells per plant for analyses. (ejbiotechnology.info)
  • I am extracting DNA from plants that have been exposed to contaminants, then using restriction enzymes to see if there is any change. (protocol-online.org)
  • A method is presented for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length) which is free of contaminants which interfere with complete digestion by restriction endonucleases. (nih.gov)
  • The purified DNA is ready for use in subsequent reactions such as PCR, Southern blotting, and restriction analysis. (clontech.com)
  • DNA suitable for digestion with restriction endonucleases has been isolated from Theobroma cacao , a plant high in polyphenolic compounds, and several other plant taxa using the method presented in this paper. (springer.com)
  • Purified DNA is suitable for PCR, restriction enzyme digestion, and hybridization techniques. (omegabiotek.com)
  • Purified DNA is suitable for PCR, restriction digestion, next generation sequencing, and hybridization applications. (omegabiotek.com)
  • Scientists have discovered that a carnivorous plant deletes so much of its own junk DNA that it has hardly any left. (latimes.com)
  • The finding, published online in Nature, hints that such noncoding DNA may not be as important as some scientists believe. (latimes.com)
  • But it does show that perhaps all that junk DNA - which some scientists have argued serves some undiscovered purpose - may be getting more credit than is due, in humans as well as plants. (latimes.com)
  • In a National Science Foundation -funded study, scientists explore this question through the evolution of the gardenia, Gardenia jasminoides , an evergreen shrub with white flowers planted as an ornamental in the tropics. (nsf.gov)
  • be assigned a simple DNA bar code so it would be easy to tell them apart" as written in Scanning Life (National Geographic, May 2010)), which is present in the mitochondrial DNA of every multi-cellular organism, scientists are able to readily determine phylogeny (identification) on a molecular level and store it in databases for easy retrieval. (colorbasepair.com)
  • A new paper from scientists in North America, Europe and China reveals important details about key transitions in the evolution of plant life on our planet. (umich.edu)
  • Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. (springer.com)
  • First author Onur Oztas, PhD, a postdoctoral researcher in the Sancar lab, said, "DNA damage accumulating in a plant will impair its growth and development, so boosting the excision repair system could be a good strategy for improving crop yields. (eurekalert.org)
  • Typical yields range from 20 to 80 µg of DNA for NucleoSpin Plant Midi, and 60 to 260 µg of DNA for NucleoSpin Plant Maxi, depending on the size and source of the sample. (clontech.com)
  • The silica membrane in the columns is optimized to improve DNA binding to give high-yields of high-quality DNA, even from difficult samples such as freeze-dried budding leaves. (bioline.com)
  • Using the high-throughput in vitro approach SELEX-seq (systematic evolution of ligands by exponential enrichment followed by high-throughput DNA sequencing), the authors characterized the DNA binding preferences of three MADS domain-containing transcription factors that are required for floral organ formation: APETALA1 (AP1), SEPALLATA3 (SEP3), and AGAMOUS (AG). (plantcell.org)
  • High-throughput isolation of DNA from plant leaf samples for PCR-based analysis, e.g. (lucigen.com)
  • The U.S. Justice Department charged DNA Plant Technology with one misdemeanor count of conspiracy to violate the Tobacco Seed Export law, prohibiting the export of tobacco seeds without a permit (a law which was repealed in 1991). (wikipedia.org)
  • Meloidogyne, Globodera and Heterodera genera have the most cosmopolitan distribution and destructive effects on virtually all crop plants (Hyman and Powers, 1991). (springer.com)
  • Sancar, the Sarah Graham Kenan Professor of Biochemistry and Biophysics, was awarded the 2015 Nobel Prize in Chemistry for his studies of excision repair, which is now widely viewed as the major mechanism of DNA repair - including repair of UV damage - in living organisms. (eurekalert.org)
  • There would likely be no bladderwort had there been no junk DNA in its ancestral line and other findings point to such noncoded DNA as necessary for evolution [slashdot.org]. (slashdot.org)
  • 6. 23S rDNA-Derived Small Ribosomal RNAs: Their Structure and Evolution with References to plant Phylogeny. (crcpress.com)
  • It targets one of the major moments in plant evolution, when the ancestors of most of the world's flowering plants split into two major groups. (innovations-report.com)
  • This paper and others show flowering plants as layer after layer of bursts of evolution," said Doug Soltis, study co-author and UF distinguished professor of biology. (innovations-report.com)
  • 2005. Evolution of DNA amounts across land plants (Embryophyta). (wikipedia.org)
  • The day has hardly begun and already you have dabbled in some of the great mysteries of plant evolution. (newscientist.com)
  • Throughout evolution ?in the wild and in crops cultivated by humans ?plants have developed systems for resisting the attack of microbial pathogens, while these microbes themselves have depended on their ability to alter molecular attack strategies in order to flourish. (bio-medicine.org)
  • These findings offer a molecular explanation for how exposure to plant resistance mechanisms can directly drive the evolution of new virulent forms of a bacterial pathogen. (bio-medicine.org)
  • Stanley Cohen speaks about his and Herbert Boyer's experiment to make the first plasmid that had been engineered to contain foreign DNA. (dnalc.org)
  • This construct was then introduced into a plasmid vector, initially pART and later pMDC7, and then transformed into plants using Agrobacterium. (unl.edu)
  • Criminals can remove the dye with proper cleaning, but the long strands of plant DNA remained and allowed police to easily identity the culprits using handheld scanners. (popsci.com)
  • Now, they're considering a novel approach to give legit wares a mark of distinction: embed them with strands of plant DNA. (wired.com)
  • The finding overturns the notion that this repetitive, non-coding DNA, popularly called 'junk' DNA, is necessary for life. (slashdot.org)
  • Two out of 15 primers yielded fragments of 500 and 730 bp which were detected in all male plants but not in any of the female plants tested. (nii.ac.jp)
  • These two DNA fragments were cloned and used as probes in gel blot analysis of genomic DNA. (nii.ac.jp)
  • These DNA fragments are presumably, entering the blood stream from the gut and then from there reaching the udder and the milk. (testbiotech.org)
  • In the past, several experts and also the European Food Safety Authority EFSA were of the opinion that specific DNA fragments related to transgenic material, could not be detected in animals. (testbiotech.org)
  • Kasamatsu H and Nakanishi A (1998) How do animal DNA viruses get to the nucleus? (els.net)
  • Krajcsi P and Wold WS (1998) Inhibition of tumor necrosis factor and interferon triggered responses by DNA viruses. (els.net)
  • DNA Plant Technology pleaded guilty in 1998 and agreed to cooperate with further investigations of Brown & Williamson. (wikipedia.org)
  • Schubbert R., Hohlweg U., Renz D., Doerfler W. (1998) On the fate of orally ingested foreign DNA in mice: chromosomal association and placental transmission to the fetus, Molecular Genetics and Genomics 259: 569-576. (testbiotech.org)
  • In 1995, DNA Plant Technology unveiled a second generation of a different transgenic tomato and served it at a meeting of its shareholders. (wikipedia.org)
  • Bennett and Leitch, 1995, 1997, 2005 and 2011) the non-standard plant names are retained in this database and are used when searches select the Traditional family name. (kew.org)
  • Most of junk DNA is. (slashdot.org)
  • Do humans need junk DNA? (latimes.com)
  • Do humans need mystery 'junk' DNA? (latimes.com)
  • Junk DNA is probably well named as junk. (latimes.com)
  • By extension, I would say it's suggestive that maybe junk DNA in general isn't of much importance. (latimes.com)
  • Your Face is Made of Junk DNA! (wn.com)
  • The generation of transgenic apple plants relies on the molecular analysis of transgene integration and expression based on polymerase chain reaction (PCR) analysis, blotting techniques and enzymatic assays on vitro leaves of putative transgenic regenerates. (ejbiotechnology.info)
  • Plants were evaluated using standard molecular techniques, such as PCR, Southern blot, reverse transcription PCR (RT-PCR) and Enzyme Linked Immunosorbent Assay (ELISA), and propagated in vitro on non-selective antibiotic-free media for four years to mimic natural conditions in the field. (ejbiotechnology.info)
  • AtTBP1 is an Arabidopsis thaliana protein that binds plant telomeric DNA in vitro. (ovid.com)
  • ISOLATE II Plant DNA Kit shows excellent recovery of plant DNA when different homogenization techniques are used. (bioline.com)
  • The ISOLATE II Plant DNA Kit is able to handle these kinds of samples and worked well for soil samples. (bioline.com)
  • Please refer to the ISOLATE II Selection Chart to confirm the applications for which the ISOLATE II Plant DNA Kit is recommended. (bioline.com)
  • Optional on-column DNase and RNase treatments provide flexibility to isolate DNA-free RNA or RNA-free DNA respectively. (norgenbiotek.com)
  • Together, these findings reveal a role for the DNA repair factor DDB2 in shaping the Arabidopsis DNA methylation landscape in the absence of applied genotoxic stress. (plantcell.org)
  • PCR amplification of a 500 bp product isolated from genomic DNA. (sigmaaldrich.com)
  • DNA was isolated from male and female plants and subjected to random amplification of polymorphic DNA. (nii.ac.jp)
  • 1 Laboratory for Plant Molecular Biology, Rockefeller University, New York, NY 10021-6399. (nih.gov)
  • Although chromosome painting is used throughout biology, especially in medicine, molecular biology and genetics, some of the most dramatic results are coming from plant science. (newscientist.com)
  • A binary-BAC (BIBAC) vector suitable for Agrobacterium-mediated plant transformation with high-molecular-weight DNA was constructed. (nih.gov)
  • The plant cell is contacted with the zoospore under conditions suitable for transmission of the nucleoprotein complex into the cell. (google.com)
  • I believe a more prudent falsifiable hypothesis would run along the lines of (and I'm sorry, I'm only a software developer): Due to relaxed external selective pressures the bladderwort's RNA polymerase has become adept at writing coding errors to the 3% noncoded DNA during replication and this actually still serves a vital function -- especially if the bladderwort is to survive in a much larger window than a few generations. (slashdot.org)
  • They frequently subvert cellular pathways involved in transcription, translation, DNA replication and cellular defence. (els.net)
  • General steps during virus DNA replication. (els.net)
  • Boehmer PE and Lehman IR (1997) Herpes simplex virus DNA replication. (els.net)
  • Gutierrez C (1999) Geminivirus DNA replication. (els.net)
  • The biochemistry of DNA replication 3. (routledge.com)
  • Control of DNA replication 4. (routledge.com)
  • Replication of plant organelle DNA 5. (routledge.com)
  • Replication of DNA viruses in plants 6. (routledge.com)
  • List and explain the mechanisms that insure high-fidelity DNA replication 2. (coursehero.com)
  • Arimura is an expert in plant molecular genetics at the University of Tokyo and led the research team, whose results were published in Nature Plants. (eurekalert.org)
  • The plants used for this study were Arabidopsis thaliana , which have been shown to be useful in modeling plant genetics. (unl.edu)
  • To authenticate an object, they extract DNA from the object (i.e., the pool of reverse primers) and mix it together with their single forward primer, template, and standard PCR reagents. (slashdot.org)
  • The main objective of various DNA isolation methods is development of relatively quick, inexpensive, and consistent protocol to extract high-quality DNA with better yield. (intechopen.com)
  • DNA, Plant" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • RNase sensitivity of the DNA-protein interactions was tested to investigate whether an RNA component mediates the telomeric DNA-protein interaction. (deepdyve.com)
  • Our results suggest that AtTBP1 is involved in the telomere length mechanism in A. thaliana and that the Myb-extension domain of AtTBP1 may stabilize plant telomeric DNA binding. (ovid.com)
  • DNA cytosine methylation can be maintained between generations and this may be important for accelerated adaption to stress conditions. (scirp.org)
  • Thank you for your interest in spreading the word on Plant Physiology. (plantphysiol.org)
  • Message Body (Your Name) thought you would like to see the Plant Physiology web site. (plantphysiol.org)
  • Sysmex Partec is a leading manufacturer of complete solutions in industrial applications and the only one producing reagents for the analysis of plant DNA, with many years of expertise in this field. (selectscience.net)
  • If you work in a company or research institute in plant breeding and ecology, using CyStain™ UV / PI OxProtect will make your life easier since you no longer have to prepare reagents, add additives to the reagents that have been on the market for many years, or accept the oxidative effect. (selectscience.net)
  • CHAPEL HILL, NC - If the ultraviolet radiation from the sun damages human DNA to cause health problems, does UV radiation also damage plant DNA? (eurekalert.org)
  • This implies that excision repair is needed to fix DNA damage from other, unknown factors besides UV," Oztas said. (eurekalert.org)
  • Oxidation caused by metal oxides has been shown to induce DNA damage in certain organisms. (innovations-report.com)
  • Copper oxide nanoparticle mediated DNA damage in terrestrial plant models. (innovations-report.com)
  • The living plant DNA reportedly repairs damage to the skin's DNA caused by environmental stresses. (cosmeticsandtoiletries.com)
  • Here, we uncover a previously unrecognized interplay between the DNA repair factor DNA DAMAGE BINDING PROTEIN2 (DDB2) and the DNA methylation machinery in Arabidopsis thaliana . (plantcell.org)
  • As photosynthetic organisms, plants need to prevent light and more specifically UV irradiation from inducing a large set of irreversible DNA damage. (plantcell.org)
  • The DNA DAMAGE BINDING2 (DDB2) protein is a key factor for the recognition of UV-induced DNA lesions during GGR . (plantcell.org)
  • Damage was analyzed as DNA strand breaks in cell cultures of Pisum sativum (garden pea), Daucus carota (carrot), Populus tremula L. × P. tremuloides (hybrid aspen) and Catharanthus roseus (Madagascar periwinkle), monitored by single cell gel electrophoresis (comet assay), and assays of cell leakage in C. roseus. (diva-portal.org)
  • Neither TRIG nor poly(ADP-ribose) polymerase (PARP) inhibitor 3-aminobenzamide offered any protection against DNA damage or cell leakage, nor did they promote aconitase or fumarase activities, or glutathione metabolism. (diva-portal.org)
  • In particular, acetylation of histones H3 and H4 plays an important role in the mammalian and yeast DNA damage response and survival under genotoxic stress. (biochemj.org)
  • However, the role of post-translational modifications to histones during the plant DNA damage response is currently poorly understood. (biochemj.org)
  • Immunoblot analysis revealed an increase in the relative abundance of the H3 acetylated N-terminus, and a global decrease in hyperacetylation of H4 in response to DNA damage induced by X-rays. (biochemj.org)
  • Conversely, mutants in the key DNA damage signalling factor ATM (ATAXIA TELANGIECTASIA MUTATED) display increased histone acetylation upon irradiation, linking the DNA damage response with dynamic changes in histone modification in plants. (biochemj.org)
  • Joan Ruddock, Minister for Climate Change and Biodiversity said: "This is a great breakthrough that could save many endangered plants. (bio-medicine.org)
  • The database was originally launched as the "Angiosperm DNA C-values Database" in April 1997, essentially as an online version of collected data lists that had been published by Prof. Bennett and colleagues since the 1970s. (wikipedia.org)
  • These effects occur in about 25% of all cases and affect key agricultural plants such as maize, sugar beet, rice and bananas, and also many ornamental plants. (selectscience.net)
  • From strange and exotic algae, mosses, ferns, trees and flowers growing deep in steamy rain forests to the grains and vegetables we eat and the ornamental plants adorning our homes, all plant life on Earth shares more than a billion years of history. (umich.edu)
  • The plant mitochondria rapidly moving around the cell (Arabidopsis leaf epidermal cell) in this video were artificially made to glow green, but are shown at their actual speed. (eurekalert.org)
  • Amount of DNA per mg of leaf sample shown above. (omegabiotek.com)
  • A new approach to the evaluation of the relative degree of genomic DNA methylation through the quantification of 2'-deoxynucleosides is proposed. (nih.gov)
  • Detection and quantification of 5-methyl 2'-deoxycytidine in genomic DNA has been performed using micellar high-performance capillary electrophoresis (HPCE) with UV-Vis detection. (nih.gov)
  • This approach has been demonstrated to be more sensitive and specific than other HPCE methods for the quantification of DNA methylation degree and also to be faster than other HPLC-based methods. (nih.gov)
  • DNA quantification confirmed via SYBR® qPCR (data not shown). (omegabiotek.com)
  • 1980. Rapid isolation of high molecular weight plant DNA. (springer.com)