Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
A family of structurally-related DNA helicases that play an essential role in the maintenance of genome integrity. RecQ helicases were originally discovered in E COLI and are highly conserved across both prokaryotic and eukaryotic organisms. Genetic mutations that result in loss of RecQ helicase activity gives rise to disorders that are associated with CANCER predisposition and premature aging.
A family of proteins that promote unwinding of RNA during splicing and translation.
An autosomal recessive disorder characterized by telangiectatic ERYTHEMA of the face, photosensitivity, DWARFISM and other abnormalities, and a predisposition toward developing cancer. The Bloom syndrome gene (BLM) encodes a RecQ-like DNA helicase.
A large family of RNA helicases that share a common protein motif with the single letter amino acid sequence D-E-A-D (Asp-Glu-Ala-Asp). In addition to RNA helicase activity, members of the DEAD-box family participate in other aspects of RNA metabolism and regulation of RNA function.
An autosomal recessive disorder that causes premature aging in adults, characterized by sclerodermal skin changes, cataracts, subcutaneous calcification, muscular atrophy, a tendency to diabetes mellitus, aged appearance of the face, baldness, and a high incidence of neoplastic disease.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
A family of enzymes that catalyze the exonucleolytic cleavage of DNA. It includes members of the class EC 3.1.11 that produce 5'-phosphomonoesters as cleavage products.
A family of DNA helicases that participate in DNA REPLICATION. They assemble into hexameric rings with a central channel and unwind DNA processively in the 5' to 3' direction. DnaB helicases are considered the primary replicative helicases for most prokaryotic organisms.
The process by which a DNA molecule is duplicated.
A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
An increased tendency of the GENOME to acquire MUTATIONS when various processes involved in maintaining and replicating the genome are dysfunctional.
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins obtained from ESCHERICHIA COLI.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A single-stranded DNA-binding protein that is found in EUKARYOTIC CELLS. It is required for DNA REPLICATION; DNA REPAIR; and GENETIC RECOMBINATION.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
DNA TOPOISOMERASES that catalyze ATP-independent breakage of one of the two strands of DNA, passage of the unbroken strand through the break, and rejoining of the broken strand. DNA Topoisomerases, Type I enzymes reduce the topological stress in the DNA structure by relaxing the superhelical turns and knotted rings in the DNA helix.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Deoxyribonucleic acid that makes up the genetic material of CHLOROPLASTS.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Proteins found in any species of bacterium.
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Enzymes that catalyze the template-directed incorporation of ribonucleotides into an RNA chain. EC 2.7.7.-.
Deoxyribonucleic acid that makes up the genetic material of fungi.
An autosomal recessive syndrome occurring principally in females, characterized by the presence of reticulated, atrophic, hyperpigmented, telangiectatic cutaneous plaques, often accompanied by juvenile cataracts, saddle nose, congenital bone defects, disturbances in the growth of HAIR; NAILS; and TEETH; and HYPOGONADISM.
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The rate dynamics in chemical or physical systems.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
An enzyme which catalyzes the hydrolysis of nucleoside triphosphates to nucleoside diphosphates. It may also catalyze the hydrolysis of nucleotide triphosphates, diphosphates, thiamine diphosphates and FAD. The nucleoside triphosphate phosphohydrolases I and II are subtypes of the enzyme which are found mostly in viruses.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A component of eukaryotic initiation factor 4F that as an RNA helicase involved in unwinding the secondary structure of the 5' UNTRANSLATED REGION of MRNA. The unwinding facilitates the binding of the 40S ribosomal subunit.
Changes in the organism associated with senescence, occurring at an accelerated rate.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A cross-shaped DNA structure that can be observed under the electron microscope. It is formed by the incomplete exchange of strands between two double-stranded helices or by complementary INVERTED REPEAT SEQUENCES that refold into hairpin loops on opposite strands across from each other.
A general transcription factor that is involved in basal GENETIC TRANSCRIPTION and NUCLEOTIDE EXCISION REPAIR. It consists of nine subunits including ATP-DEPENDENT DNA HELICASES; CYCLIN H; and XERODERMA PIGMENTOSUM GROUP D PROTEIN.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.
A single-stranded DNA-dependent RNA polymerase that functions to initiate, or prime, DNA synthesis by synthesizing oligoribonucleotide primers. EC 2.7.7.-.
A DNA helicase that is a component of TRANSCRIPTION FACTOR TFIIH. It plays an essential role in NUCLEOTIDE EXCISION REPAIR, and mutations in this protein are associated with XERODERMA PIGMENTOSUM.
The process of cleaving a chemical compound by the addition of a molecule of water.
RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.
Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. It infects E. coli and is the best known of the T-even phages. Its virion contains linear double-stranded DNA, terminally redundant and circularly permuted.

The Drosophila kismet gene is related to chromatin-remodeling factors and is required for both segmentation and segment identity. (1/5302)

The Drosophila kismet gene was identified in a screen for dominant suppressors of Polycomb, a repressor of homeotic genes. Here we show that kismet mutations suppress the Polycomb mutant phenotype by blocking the ectopic transcription of homeotic genes. Loss of zygotic kismet function causes homeotic transformations similar to those associated with loss-of-function mutations in the homeotic genes Sex combs reduced and Abdominal-B. kismet is also required for proper larval body segmentation. Loss of maternal kismet function causes segmentation defects similar to those caused by mutations in the pair-rule gene even-skipped. The kismet gene encodes several large nuclear proteins that are ubiquitously expressed along the anterior-posterior axis. The Kismet proteins contain a domain conserved in the trithorax group protein Brahma and related chromatin-remodeling factors, providing further evidence that alterations in chromatin structure are required to maintain the spatially restricted patterns of homeotic gene transcription.  (+info)

Stable remodeling of tailless nucleosomes by the human SWI-SNF complex. (2/5302)

The histone N-terminal tails have been shown previously to be important for chromatin assembly, remodeling, and stability. We have tested the ability of human SWI-SNF (hSWI-SNF) to remodel nucleosomes whose tails have been cleaved through a limited trypsin digestion. We show that hSWI-SNF is able to remodel tailless mononucleosomes and nucleosomal arrays, although hSWI-SNF remodeling of tailless nucleosomes is less effective than remodeling of nucleosomes with tails. Analogous to previous observations with tailed nucleosomal templates, we show both (i) that hSWI-SNF-remodeled trypsinized mononucleosomes and arrays are stable for 30 min in the remodeled conformation after removal of ATP and (ii) that the remodeled tailless mononucleosome can be isolated on a nondenaturing acrylamide gel as a novel species. Thus, nucleosome remodeling by hSWI-SNF can occur via interactions with a tailless nucleosome core.  (+info)

Arginine methylation and binding of Hrp1p to the efficiency element for mRNA 3'-end formation. (3/5302)

Hrp1p is a heterogeneous ribonucleoprotein (hnRNP) from the yeast Saccharomyces cerevisiae that is involved in the cleavage and polyadenylation of the 3'-end of mRNAs and mRNA export. In addition, Hrplp is one of several RNA-binding proteins that are posttranslationally modified by methylation at arginine residues. By using functional recombinant Hrp1p, we have identified RNA sequences with specific high affinity binding sites. These sites correspond to the efficiency element for mRNA 3'-end formation, UAUAUA. To examine the effect of methylation on specific RNA binding, purified recombinant arginine methyltransferase (Hmt1p) was used to methylate Hrp1p. Methylated Hrp1p binds with the same affinity to UAUAUA-containing RNAs as unmethylated Hrpl p indicating that methylation does not affect specific RNA binding. However, RNA itself inhibits the methylation of Hrp1p and this inhibition is enhanced by RNAs that specifically bind Hrpl p. Taken together, these data support a model in which protein methylation occurs prior to protein-RNA binding in the nucleus.  (+info)

Reconstitution of the transcription factor TFIIH: assignment of functions for the three enzymatic subunits, XPB, XPD, and cdk7. (4/5302)

To understand the initiation of the transcription of protein-coding genes, we have dissected the role of the basal transcription/DNA repair factor TFIIH. Having succeeded in reconstituting a functionally active TFIIH from baculovirus recombinant polypeptides, we were able to analyze the role of XPB, XPD, and cdk7 subunits in the transcription reaction. Designing mutated recombinant subunits, we show that the XPB helicase is absolutely required for transcription to open the promoter around the start site whereas the XPD helicase, which is dispensable, stimulates transcription and allows the CAK complex to be anchored to TFIIH. In addition, we also show that cdk7 may phosphorylate the carboxy-terminal domain (CTD) of RNA pol II in the absence of promoter opening.  (+info)

Plasmid replication initiator protein RepD increases the processivity of PcrA DNA helicase. (5/5302)

The replication initiator protein RepD encoded by the Staphylococcus chloramphenicol resistance plasmid pC221 stimulates the helicase activity of the Bacillus stearothermophilus PcrA DNA helicase in vitro. This stimulatory effect seems to be specific for PcrA and differs from the stimulatory effect of the Escherichia coli ribosomal protein L3. Whereas L3 stimulates the PcrA helicase activity by promoting co-operative PcrA binding onto its DNA substrate, RepD stimulates the PcrA helicase activity by increasing the processivity of the enzyme and enables PcrA to displace DNA from a nicked substrate. The implication of these results is that PcrA is the helicase recruited into the replisome by RepD during rolling circle replication of plasmids of the pT181 family.  (+info)

Evolution of the RECQ family of helicases: A drosophila homolog, Dmblm, is similar to the human bloom syndrome gene. (6/5302)

Several eukaryotic homologs of the Escherichia coli RecQ DNA helicase have been found. These include the human BLM gene, whose mutation results in Bloom syndrome, and the human WRN gene, whose mutation leads to Werner syndrome resembling premature aging. We cloned a Drosophila melanogaster homolog of the RECQ helicase family, Dmblm (Drosophila melanogaster Bloom), which encodes a putative 1487-amino-acid protein. Phylogenetic and dot plot analyses for the RECQ family, including 10 eukaryotic and 3 prokaryotic genes, indicate Dmblm is most closely related to the Homo sapiens BLM gene, suggesting functional similarity. Also, we found that Dmblm cDNA partially rescued the sensitivity to methyl methanesulfonate of Saccharomyces cerevisiae sgs1 mutant, demonstrating the presence of a functional similarity between Dmblm and SGS1. Our analyses identify four possible subfamilies in the RECQ family: (1) the BLM subgroup (H. sapiens Bloom, D. melanogaster Dmblm, and Caenorhabditis elegans T04A11.6); (2) the yeast RECQ subgroup (S. cerevisiae SGS1 and Schizosaccharomyces pombe rqh1/rad12); (3) the RECQL/Q1 subgroup (H. sapiens RECQL/Q1 and C. elegans K02F3.1); and (4) the WRN subgroup (H. sapiens Werner and C. elegans F18C5.2). This result may indicate that metazoans hold at least three RECQ genes, each of which may have a different function, and that multiple RECQ genes diverged with the generation of multicellular organisms. We propose that invertebrates such as nematodes and insects are useful as model systems of human genetic diseases.  (+info)

Molecular shape and ATP binding activity of rat p50, a putative mammalian homologue of RuvB DNA helicase. (7/5302)

Based on partial amino acid sequences of p50 purified from a high-salt buffer extract of a rat liver nuclear matrix fraction, p50 cDNA was cloned and sequenced, and its amino acid sequence was predicted. The sequence contained helicase motifs, and showed homology with RuvB DNA helicase of Thermus thermophilus and an open reading frame for an unknown 50.5 k protein of Saccharomyces cerevisiae. p50 was expressed as a GST-fusion protein and antiserum against the protein was generated. p50 was localized to the nuclear matrix by cell fractionation and immunoblotting. p50 bound to ATP-Sepharose beads. Ultracentrifugation and gel filtration analyses showed that p50 in rat liver and Xenopus egg mitotic extracts exists as large complexes corresponding to 697 k and 447 k, respectively. A 50 k protein reactive with p50 antibodies was detected not only in rat liver nuclei, but also in a Xenopus egg cytoplasm fraction and a S. cerevisiae extract. This suggests that this putative DNA helicase is present in a wide variety of species ranging from yeast to mammals.  (+info)

Yeast and human genes that affect the Escherichia coli SOS response. (8/5302)

The sequencing of the human genome has led to the identification of many genes whose functions remain to be determined. Because of conservation of genetic function, microbial systems have often been used for identification and characterization of human genes. We have investigated the use of the Escherichia coli SOS induction assay as a screen for yeast and human genes that might play a role in DNA metabolism and/or in genome stability. The SOS system has previously been used to analyze bacterial and viral genes that directly modify DNA. An initial screen of meiotically expressed yeast genes revealed several genes associated with chromosome metabolism (e.g., RAD51 and HHT1 as well as others). The SOS induction assay was then extended to the isolation of human genes. Several known human genes involved in DNA metabolism, such as the Ku70 end-binding protein and DNA ligase IV, were identified, as well as a large number of previously unknown genes. Thus, the SOS assay can be used to identify and characterize human genes, many of which may participate in chromosome metabolism.  (+info)

TY - JOUR. T1 - DNA helicase E and DNA polymerase ∈ functionally interact for displacement synthesis. AU - Turchi, John. AU - Siegal, Gregg. AU - Bambara, Robert A.. PY - 1992. Y1 - 1992. N2 - A functional interaction between DNA helicase E and DNA polymerase ∈ from calf thymus has been detected which results in the extension of an upstream 3′ OH through a downstream primer to the end of a synthetic template. DNA synthesis resulting in full-length extension products was dependent on the addition of DNA helicase E and hydrolysis of ATP, suggesting that displacement of the downstream primer was required. Identical reactions using DNA polymerases α and δ in place of DNA polymerase ∈ showed no full-length products dependent on helicase E, indicating that polymerases α and δ were incapable of functionally interacting with the helicase. The reaction leading to full-length extension products was time dependent and dependent on the concentration of added polymerase ∈ and helicase E. ...
Excellgen Brahma-related Gene 1 protein, wild type, BRG1, SMARCA4 [RP-22] - Product Name Brahma-related Gene 1, BRG1, SMARCA4 Size 5,000 U Description The wild type human brahma-related gene 1 (Brg1) encodes a protein of 1,647 amino acids that contains a conserved domain of the SWI2/SNF2 family necessary for normal mitotic growth and transcription regulation (1-3). BRG1 is an essential component of the SWI/SNF chromatin remodeling complexes
Shop ATP-dependent helicase/deoxyribonuclease ELISA Kit, Recombinant Protein and ATP-dependent helicase/deoxyribonuclease Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Shop ATP-dependent helicase ELISA Kit, Recombinant Protein and ATP-dependent helicase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Escherichia coli DNA helicase II plays a specific role in the methyl‐directed mismatch repair and nucleotide excision repair pathways (Caron et al., 1985; Husain et al., 1985; Lahue et al., 1989), a less defined role in recombination (Horii and Clark, 1973; Arthur and Lloyd, 1980; Feinstein and Low, 1986; Schellhorn and Low, 1991; Mendonca et al., 1993, 1995; Morel et al., 1993) and possibly replication (Taucher‐Scholz and Hoffman‐Berling, 1983; Washburn and Kushner, 1991; George et al., 1994; Brosh and Matson, 1995). We used the yeast two‐hybrid system to search for interacting protein partners that might direct the involvement of helicase II in one or more of these processes. The two‐hybrid screen revealed an interaction between helicase II and MutL, an essential component for methyl‐directed mismatch repair and, according to a recent report, a factor in transcription‐coupled nucleotide excision repair (Mellon and Champe, 1996).. Deletion analysis demonstrated that the ...
The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
DNA helicases are a diverse group of enzymes that separate the two strands of duplex DNA. Using the free energy derived from the hydrolysis of a 5ʹ nucleoside triphosphate, generally ATP, the helicase catalyzes the unwinding of duplex DNA to yield single-stranded DNA (ssDNA), a process that is required in replication, transcription, recombination, and repair. Thus, helicases are involved in essentially all metabolic pathways that require the separation of duplex DNA (Brosh 2013; Khan et al. 2015).. Helicases exhibit a diversity of structure and mechanism that may be related to the often unique and specialized roles that these enzymes can play in the cell (Brosh 2013; Daley et al. 2013). Importantly, distinct helicases can interact with specific DNA substrates. For example, during repair of DNA damage, different helicases often act within particular pathways and on unique DNA intermediates that are generated as repair progresses, such as Holliday junctions (HJs) or displacement loops (D-loops). ...
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Frye, Stephan Alfons; Balasingham, Seetha; Beyene, Getachew Tesfaye; Homberset, Håvard; Namouchi, Amine & Tonjum, Tone (2016). Meningococcal DNA binding and unwinding proteins. Show summary Background: DNA helicases are a ubiquitous group of enzymes that use the energy of nucleoside triphosphate (dNTP) hydrolysis to catalyze the separation of double-stranded DNA (dsDNA). Helicases are involved in essentially every step in DNA metabolism, including replication, DNA repair, recombination, transcription, Holliday junction movement, and displacement of proteins from DNA. We investigated the DNA helicases RecG and DinG from Neisseria meningitidis (NmRecG and NmDinG) and their roles during genotoxic stress, including DNA damage. These helicases belong to superfamily 2, are ATP dependent and exert 5′to 3′ directionality. Our aim was to define the potential roles of NmRecG and NmDinG in DNA repair, recombination and replication (3R). Methods and results: Cell lysates from Nm wildtype and recG and ...
PubMed journal article: Brahma-related gene 1 ameliorates the neuronal apoptosis and oxidative stress induced by oxygen-glucose deprivation/reoxygenation through activation of Nrf2/HO-1 signaling. Download Prime PubMed App to iPhone, iPad, or Android
Our group studies the process of homologous recombination. We focus on the DNA motor protein complexes or DNA nano-machines responsible for driving this process. These machines are frequently coupled to, or powered by, DNA helicases. DNA helicases are ubiquitous enzymes whose primary function is to unwind DNA duplexes into their component single strands, a process that is coupled to the hydrolysis of nucleoside 5-triposphates. Our work is aimed at understanding the biochemical mechanism of DNA helicases and how these mechanisms contribute to and are adapted to the processes of recombination and DNA repair.. To see how DNA nano-machines put it all together, we use state-of-the-art single molecule techniques to visualize in real time, the dynamic properties of DNA nan-machines that are lost in the averaging process using conventional ensemble assays. Single molecule manipulation and observation techniques provide an unparalleled and dramatic means to study biological reactions. The two techniques ...
The minichromosome maintenance (MCM) helicase is the presumptive replicative helicase in archaea and eukaryotes. The archaeal homomultimeric MCM has a two-tier structure. One tier contains the AAA+ motor domains of the proteins, and these are the minimal functional helicase domains. The second tier is formed by the N-terminal domains. These domains are not essential for MCM helicase activity but act to enhance the processivity of the helicase. We reveal that a conserved loop facilitates communication between processivity and motor tiers. Interestingly, this allostery seems to be mediated by interactions between, rather than within, individual protomers in the MCM ring.
The PriA protein of Escherichia coli plays a key role in the rescue of replication forks stalled on the template DNA. One attractive model of rescue relies on homologous recombination to establish a new fork via PriA-mediated loading of the DnaB replicative helicase at D loop intermediates. We provi …
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DNA is a helical, double-stranded molecule that bears the genetic code. During the replication of DNA, its two strands are separated from each other resulting in a configuration called the replication fork. The replication fork is comprised of two prongs wherein each prong is the single strand of DNA. The replication fork results from the work of the helicases. Helicases are enzymes that are used by living organisms to separate the strands of nucleic acids, and in this case, the double-stranded DNA. Helicases do so by breaking the hydrogen bonds that hold the two strands together. Helicases are able to do this by utilizing energy from the hydrolysis of nucleoside triphosphates (e.g. ATPs). Apart from DNA replication, helicases are also used in transcription, translation, recombination, DNA repair, and ribosome biogenesis. DNA helicases were first discovered and isolated in E.coli in 1976.1 ...
ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
Figure 6. Translocation Mechanism and Directional Polarity(A) Schematic of a Rho translocation cycle in which six ATP molecules are hydrolyzed to move six nucleotides of RNA. Helicase subunits are illustrated as colored spheres. RNA is shown as a chain of white spheres spiraling out of the plane of the paper. Protein-RNA contacts are indicated by lines connecting the protein and RNA spheres; the black RNA sphere serves as a reference point and moves toward the viewer as the boxed red subunit transitions through six steps in the translocation cycle. A yellow star represents activation of the allosteric network that likely promotes hydrolysis. See also Movies S3-S6.(B) Schematics of Rho and E1 (chains A-F) illustrating their respective sequential ATP hydrolysis directions. Protein subunits are colored as inFigure 1. Nucleic acid phosphates observed in the structures are illustrated as bold orange circles, with the incoming phosphate shown as a dashed orange circle. Rectangles represent the two ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
gi,116242792,sp,P51532.2,SMCA4_HUMAN RecName: Full=Transcription activator BRG1; AltName: Full=ATP-dependent helicase SMARCA4; AltName: Full=BRG1-associated factor 190A; Short=BAF190A; AltName: Full=Mitotic growth and transcription activator; AltName: Full=Protein BRG-1; AltName: Full=Protein brahma homolog 1; AltName: Full=SNF2-beta; AltName: Full=SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member ...
Sigal, N., Delius, H., Kornberg, T., Gefter, M. L., Alberts, B. (December 1972) A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases. Proc Natl Acad Sci U S A, 69 (12). pp. 3537-41. ...
DNA helicase. Molecular model of a helicase molecule from the SV40 virus. Helicases are enzymes that separate the two strands of the DNA double helix, by breaking the hydrogen bonds between nucleotide bases. Separation of the strands is needed for replication, and therefore this protein is vital for the ability of the virus to infect host cells. - Stock Image F006/9426
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
RuvBL1 helicase enzyme, molecular model. Helicases are enzymes that carry out several roles, primarily separating the two strands of the DNA (deoxyribonucleic acid) double helix, by breaking the hydrogen bonds between nucleotide bases. RuvBL1 plays important roles in the c-Myc and Wnt signalling pathways that are vital to chromatin remodelling, and transcriptional and developmental regulation. It also plays roles in DNA repair and apoptosis (programmed cell death). Here, it forms a cyclic hexamer, with six monomers highlighted by the colours used here. - Stock Image C015/4304
7 MemProtMD simulations of ATP-dependent helicase activity in a lipid bilayer at both coarse-grained and atomistic respresentation, including both file download and analysis. Classification based on GO GO:0008026.
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Abstract : To trigger transcription termination, the ring-shaped RNA-DNA helicase Rho from Escherichia coli chases the RNA polymerase along the nascent transcript, starting from a single-stranded C-rich Rut (Rho utilization) loading site. In some instances, a small hairpin structure divides harmlessly the C-rich loading region into two smaller Rut subsites, best exemplified by the tR1 terminator from phage lambda. Here, we show that the Rho helicase can also elude a RNA structural block located far downstream from the single-stranded C-rich region but upstream from a reporter RNA-DNA hybrid.. ...
ATPase and helicase activities of Brr2 mutants. (a) Locations of residues corresponding to brr2-1 (E610G) and brr2-R1107A on the Hel308 structure (PDB ID 2P6R 2
Rabbit anti LGP2 (N-Terminal) antibody recognizes human Probable ATP-dependent helicase LGP2 (LGP2), also known as DHX58, a 768aa cytoplas
K14439 SMARCAD1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 [EC:3.6.4.12 ...
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Streptococcus suis Holliday junction ATP-dependent DNA helicase RuvA (ruvA) datasheet and description hight quality product and Backed by our Guarantee
A plethora of mutations in chromatin regulators in diverse human cancers is emerging, attesting to the pivotal role of chromatin dynamics in tumorigenesis. A recurrent theme is inactivation of the chromodomain helicase DNA-binding (CHD) family of proteins-ATP-dependent chromatin remodelers that govern the cellular machinerys access to DNA, thereby controlling fundamental processes, including transcription, proliferation, and DNA damage repair. This review highlights what is currently known about how genetic and epigenetic perturbation of CHD proteins and the pathways that they regulate set the stage for cancer, providing new insight for designing more effective anti-cancer therapies.. ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008 ...
TY - JOUR. T1 - DNA-RNA helicase activity of RAD3 protein of Saccharomyces cerevisiae. AU - Bailly, Véronique. AU - Sung, Patrick. AU - Prakash, Louise. AU - Prakash, Satya. PY - 1991. Y1 - 1991. N2 - The RAD3 gene of Saccharomyces cerevisiae is required for excision repair of UV-damaged DNA and is essential for cell viability. The RAD3 protein exhibits a remarkable degree of sequence homology to the human excision repair protein ERCC2. The RAD3 protein is a single-stranded DNA-dependent ATPase and a DNA helicase capable of denaturing long regions of duplex DNA. Here, we demonstrate that RAD3 also possesses a potent DNA-RNA helicase activity similar in efficiency to its DNA helicase activity. The rad3 Arg-48 mutant protein, which binds but does not hydrolyze ATP, lacks the DNA-RNA unwinding activity, indicating a dependence on ATP hydrolysis. RAD3 does not show any RNA-dependent NTPase activity and, as expected, does not unwind duplex RNA. This observation suggests that RAD3 translocates on DNA ...
BACKGROUND: The HRDC (helicase and RNaseD C-terminal) domain is found at the C terminus of many RecQ helicases, including the human Werner and Bloom syndrome proteins. RecQ helicases have been shown to unwind DNA in an ATP-dependent manner. However, the specific functional roles of these proteins in DNA recombination and replication are not known. An HRDC domain exists in both of the human RecQ homologues that are implicated in human disease and may have an important role in their function. RESULTS: We have determined the three-dimensional structure of the HRDC domain in the Saccharomyces cerevisiae RecQ helicase Sgs1p by nuclear magnetic resonance (NMR) spectroscopy. The structure resembles auxiliary domains in bacterial DNA helicases and other proteins that interact with nucleic acids. We show that a positively charged region on the surface of the Sgs1p HRDC domain can interact with DNA. Structural similarities to bacterial DNA helicases suggest that the HRDC domain functions as an auxiliary ...
The S. cerevisiae yeast transcriptional activator SNF2/SWI2 protein enzymes consist of two alpha/beta-lobes similar to SF2 helicases exons 7 and 8 that activates homeotic genes designated BRG1 (brm/SWI2-related gene-1) (BAF) complexes locus: 19p13.2, [§§], express two homologs of the SWI2 subunit. A conserved ATP binding site residue in BRG1 display conventional chromatin-remodeling activity and genomic Left-handed Z-DNA stability (the helicase-like screw motion of DNA motor proteins of human SWI/SNF along the active site.) and enhanced the DNA strand-exchange activity, whereas transcription requires, in addition, an activation domain intergenic solitary (LTRs) long terminal repeat subunits of Pol IVb are targeted, to successfully culminate the repair, indicate a communication and compensation between Brm and Brg-1. BRCA1 can directly interact with the BRG1. BRCA1 is associated with a SWI/SNF-related complex SMARCA4 and the tumor suppressor SMARCB1 the hSNF5/INI1 gene, allowing access to ...
Silva AP, Ryan DP, Galanty Y, Low JK, Vandevenne M, Jackson SP, Mackay JP. Journal of Biological Chemistry 291, 924-938. Chromodomain Helicase DNA-binding protein 4 (CHD4) is a chromatin-remodeling enzyme that has been reported to regulate DNA damage responses through its N-terminal region in a poly(ADP-ribose) polymerase dependent manner. We have identified and determined the structure of a stable domain (CHD4-N) in this N-terminal region.
Padilla-Benavides T, Reyes-Gutierrez P, Imbalzano AN. Regulation of the Mammalian SWI/SNF Family of Chromatin Remodeling Enzymes by Phosphorylation during Myogenesis. Biology (Basel). 2020 Jul 03; 9(7 ...
In order to maintain cellular viability and genetic integrity cells must respond quickly following the induction of cytotoxic double strand DNA breaks (DSB). This response requires a number of processes including stabilisation of the DSB, signalling of the break and repair. It is becoming increasingly apparent that one key step in this process is chromatin remodelling. Here we describe the chromodomain helicase DNA-binding protein (CHD4) as a target of ATM kinase. We show that ionising radiation (IR)-induced phosphorylation of CHD4 affects its intranuclear organization resulting in increased chromatin binding/retention. We also show assembly of phosphorylated CHD4 foci at sites of DNA damage, which might be required to fulfil its function in the regulation of DNA repair. Consistent with this, cells overexpressing a phospho-mutant version of CHD4 that cannot be phosphorylated by ATM fail to show enhanced chromatin retention after DSBs and display high rates of spontaneous damage. These results provide
In order to maintain cellular viability and genetic integrity cells must respond quickly following the induction of cytotoxic double strand DNA breaks (DSB). This response requires a number of processes including stabilisation of the DSB, signalling of the break and repair. It is becoming increasingly apparent that one key step in this process is chromatin remodelling. Here we describe the chromodomain helicase DNA-binding protein (CHD4) as a target of ATM kinase. We show that ionising radiation (IR)-induced phosphorylation of CHD4 affects its intranuclear organization resulting in increased chromatin binding/retention. We also show assembly of phosphorylated CHD4 foci at sites of DNA damage, which might be required to fulfil its function in the regulation of DNA repair. Consistent with this, cells overexpressing a phospho-mutant version of CHD4 that cannot be phosphorylated by ATM fail to show enhanced chromatin retention after DSBs and display high rates of spontaneous damage. These results provide
XPB helicase is an integral part of transcription factor TFIIH, required for both transcription initiation and nucleotide excision repair (NER). Along with the XPD helicase, XPB plays a crucial but only partly understood role in defining and extending the DNA repair bubble around lesions in NER. Archaea encode clear homologues of XPB and XPD, and structural studies of these proteins have yielded key insights relevant to the eukaryal system. Here we show that archaeal XPB functions with a structure-specific nuclease, Bax1, as a helicase-nuclease machine that unwinds and cleaves model NER substrates. DNA bubbles are extended by XPB and cleaved by Bax1 at a position equivalent to that cut by the XPG nuclease in eukaryal NER. The helicase activity of archaeal XPB is dependent on the conserved Thumb domain, which may act as the helix breaker. The N-terminal damage recognition domain of XPB is shown to be crucial for XPB-Bax1 activity and may be unique to the archaea. These findings have implications ...
Although Pif1 helicase family members are highly similar throughout their helicase domains, their amino termini are diverse. For example, the two S. cerevisiae Pif1 family members, Pif1p and Rrm3p, are 60% similar within their ∼450-amino-acid helicase domain but have no significant similarity within their ∼250-amino-acid amino termini (Figure 1). Since a truncated Rrm3p that lacks the first 194 amino acids of the protein has ATPase and 5′ to 3′ DNA helicase activity, the amino terminus is not required for in vitro activity (Ivessa et al. 2002). This article examines the in vivo function of the Rrm3p amino terminus. We constructed a series of alleles that lacked all or part of the amino terminus of Rrm3p (Figure 2A) and tested their ability to support fork progression at three Rrm3p-dependent loci, to suppress activation of DNA checkpoints, and to maintain viability in the absence of checkpoint and repair genes.. By each of these in vivo assays, the amino terminus of Rrm3p was essential ...
PO debconf template translation of kismet # Copyright (C) 2013 THE kismetS COPYRIGHT HOLDER # This file is distributed under the same license as the kismet package. # José de Figueiredo ,[email protected],, 2013. # msgid msgstr Project-Id-Version: kismet\n Report-Msgid-Bugs-To: [email protected]\n POT-Creation-Date: 2012-12-15 14:36+0100\n PO-Revision-Date: 2013-07-15 08:19-0300\n Last-Translator: José de Figueiredo ,[email protected],\n Language-Team: Brazilian Portuguese ,[email protected],\n Language: \n MIME-Version: 1.0\n Content-Type: text/plain; charset=UTF-8\n Content-Transfer-Encoding: 8bit\n #. Type: boolean #. Description #: ../kismet.templates:2001 msgid Install Kismet \setuid root\? msgstr Instalar o Kismet com \setuid root\? #. Type: boolean #. Description #: ../kismet.templates:2001 msgid Kismet needs root privileges for some of its functions. However, running it as root (\sudo kismet\) is ...
A family of structurally-related DNA helicases that play an essential role in the maintenance of genome integrity. RecQ helicases were originally discovered in E COLI and are highly conserved across both prokaryotic and eukaryotic organisms. Genetic mutations that result in loss of RecQ helicase activity gives rise to disorders that are associated with CANCER predisposition and premature aging ...
Subunits of mammalian SWI/SNF (mSWI/SNF or BAF) complexes have recently been implicated as tumor suppressors in human malignancies. To understand the full extent of their involvement, we conducted a proteomic analysis of endogenous mSWI/SNF complexes, which identified several new dedicated, stable s …
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Manganese atom in PDB 3dkx: Crystal Structure of the Replication Initiator Protein Encoded on Plasmid PMV158 (Repb), Trigonal Form, to 2.7 Ang Resolution
Helicase loader protein (gp59) was found to form a hexamer on forked DNA and the presence of gp32 and gp41 increased the stability of the formed complex.
SWISS-MODEL Repository entry for B3WC55 (RUVA_LACCB), Holliday junction ATP-dependent DNA helicase RuvA. Lactobacillus casei (strain BL23)
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FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008 ...
Structures of this kind are often considered to occur in RNA, but if they also occur in single-stranded DNA, it raises interesting questions. If a gene has an energetically stable strands-apart configuration, getting the strands of duplex B-form DNA to separate might not be so hard. But more to the point, getting the self-annealing gene to come back together again as duplex DNA will require significant energy input. In molecular genetics, were accustomed to the idea of duplex DNA requiring help from an ATP-dependent helicase to open up (unwind) the double helix in preparation for replication or transcription. The above diagram suggests that the problem isnt opening up a gene; the greater problem may be bringing the strands together again after theyve assumed a stable strands-apart secondary structure. Theres a substantial energy barrier to be overcome before the above structure can be relaxed into randomly coiling DNA ...
Human Ku heterodimeric protein composed of Ku70 and Ku80 subunits plays an important role in the non-homologous end-joining DNA repair pathway as a sensor of
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported ...
The RecQ helicase family is a group of highly conserved DNA unwinding enzymes critical in guarding genome stability in all kingdoms of life. Human RecQ homologs...
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Complete information for CHD9 gene (Protein Coding), Chromodomain Helicase DNA Binding Protein 9, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for CHD8 gene (Protein Coding), Chromodomain Helicase DNA Binding Protein 8, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The binary distribution of Mcm2‐7 across the genome prompted us to investigate genomic features that may be associated with the broad regions of high or low Mcm2‐7 levels along the chromosome. The Mcm2‐7 localization pattern relative to annotated genomic features suggested that Mcm2‐7 may be displaced by actively transcribed genes (Fig 5A). To quantitatively assess the Mcm2‐7 distribution relative to transcription units, we generated histograms of Mcm2‐7 enrichment for transcribed and non‐transcribed genes (Fig 5B) and found a bimodal pattern of Mcm2‐7 enrichment. Specifically, active genes had no or very little Mcm2‐7 signal, whereas inactive or non‐transcribed genes exhibited an elevated Mcm2‐7 signal (P , 1.02 × 10−257; t = 40.16). We also considered that the bimodal distribution of Mcm2‐7 enrichment between active and inactive genes might be due to the activation of early origins that are enriched near actively transcribed genes. However, we found that the bimodal ...
CHD1 - CHD1 (untagged)-Human chromodomain helicase DNA binding protein 1 (CHD1) available for purchase from OriGene - Your Gene Company.
Staphylococcus aureus; strain: USA300_FPR3757; locus tag: SAUSA300_0016 (SAUSA300_RS00085); symbol: dnaC; product: replicative DNA helicase
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3 direction only. In this schematic, the replication fork (RF) is shown as opening to the right. On the lower leading strand, synthesis is continuous because extension of a single RNA primer occurs without interruption into the replication fork as it continues to open to the right. On the upper lagging strand, synthesis is discontinuous, since new RNA primers must be added as opening of the replication fork continues to expose new template. This produces a series of disconnected Okazaki fragments ...
Background The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to...
DEAD/H box protein 13 in major histocompatibility class III region (see [email protected]), recognized by monoclonal antibody 170A1 with homology to human KIAA052,(superfamily II ...
This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014] ...
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate tra
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Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 39) ...
A replisome is any of the sites on the matrix of a cell nucleus that contains enzyme complexes in which DNA replication occurs. It is located at the rep...
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A mechanistic study of helicases with magnetic traps. Hodeib S, Raj S, Manosas M, Zhang W, Bagchi D, Ducos B, Fiorini F, Kanaan J, Le Hir H,
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DNA) and ribonucleic acid(RNA) helicases are organised into six SuperFamilies (SF) of enzymes using sequence alignments, ... DNA. unwinding catalysed by hexameric DNA. helicases. (a) The bacteriophage T7 gp4A′ replicative helicase (Yu et al., ). A ... DNA. follows different paths during DNA. unwinding by nonhexameric DNA. helicases. (a) The UvrD monomer. The enzyme is shown as ... DNA, NTP binding and hydrolysis, and NTP hydrolysis‐coupled, polar unwinding of duplex DNA. DNA helicases participate in every ...
RecB is a helicase with a 3-5 directionality.. *Fungal srs2 proteins, an ATP-dependent DNA helicase involved in DNA repair. ... UvrD-like DNA helicases unwind DNA with a 3-5 polarity [PMID: 10679457]. Crystal structures of several uvrD-like DNA ... UvrD-like DNA helicase, C-terminal (IPR014017). Short name: DNA_helicase_UvrD-like_C ... Some proteins that belong to the uvrD-like DNA helicase family are listed below: *Bacterial UvrD helicase. It is involved in ...
DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free ... molecule approaches enable us to address detailed mechanistic questions about how such enzymes move processively along DNA. ... DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free ... The generic and versatile nature of the assay makes it applicable to a variety of DNA helicases and DNA templates. The method ...
Gene Ontology Term: DNA helicase activity. GO ID. GO:0003678 Aspect. Molecular Function. Description. Unwinding of a DNA helix ... ATP-dependent DNA helicase activity View GO Annotations in other species in AmiGO ...
F-box DNA helicase 1. Names. F-box only protein, helicase, 18. F-box protein, helicase, 18. NP_001245381.1. *EC 3.6.4.12 ... FBH1 F-box DNA helicase 1 [Homo sapiens] FBH1 F-box DNA helicase 1 [Homo sapiens]. Gene ID:84893 ... It contains an F-box motif and seven conserved helicase motifs, and has both DNA-dependent ATPase and DNA unwinding activities ... F-box DNA helicase 1provided by HGNC. Primary source. HGNC:HGNC:13620 See related. Ensembl:ENSG00000134452 MIM:607222; Vega: ...
The Escherichia coli recQ gene encodes a DNA helicase with an activity that unwinds duplex DNA in the 3′-to-5′ direction (1), ... RecQ Helicase Stimulates Both DNA Catenation and Changes in DNA Topology by Topoisomerase III ... Single-molecule visualization of RecQ helicase reveals DNA melting, nucleation, and assembly are required for processive DNA ... Werner Syndrome Protein: I. DNA HELICASE AND DNA EXONUCLEASE RESIDE ON THE SAME POLYPEPTIDE ...
Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity. UniProt ...
Compare DNA Helicase B ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and ... Your search returned 3 DNA Helicase B ELISA ELISA Kit across 2 suppliers. ... Human DNA helicase B, HELB ELISA Kit *Detection Target: helicase (DNA) B ... Mouse DNA helicase B, HELB ELISA Kit *Detection Target: helicase (DNA) B ...
DNA helicase II [Escherichia coli str. K-12 substr. MG1655] DNA helicase II [Escherichia coli str. K-12 substr. MG1655]. gi, ... DNA helicase II [Escherichia coli str. K-12 substr. MG1655]. GenBank: AAA67609.1 ...
IPR007694, DNA_helicase_DnaB-like_C. IPR007693, DNA_helicase_DnaB-like_N. IPR016136, DNA_helicase_N/primase_C. IPR027417, P ... IPR007694, DNA_helicase_DnaB-like_C. IPR007693, DNA_helicase_DnaB-like_N. IPR016136, DNA_helicase_N/primase_C. IPR027417, P ... tr,A0A6B8RMD4,A0A6B8RMD4_9BACL DNA helicase OS=Paenibacillus psychroresistens OX=1778678 GN=EHS13_20075 PE=3 SV=1 ... HelicaseImported. ,p>Information which has been imported from another database using automatic procedures.,/p> ,p>,a href="/ ...
Browse our DNA helicase HEL308 Protein catalog backed by our Guarantee+. ... DNA helicase HEL308 Proteins available through Novus Biologicals. ... DNA helicase HEL308 Proteins. We offer DNA helicase HEL308 Peptides and DNA helicase HEL308 Proteins for use in common research ... Our DNA helicase HEL308 Peptides and DNA helicase HEL308 Proteins can be used in a variety of model species: Human. Use the ...
... selectively bind to Myc-promoter G4-DNA-containing structure in an ATP-dependent manner. Upon G4-DNA-binding, DHX36 pulls on ... Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1; this complex exists in resting ... The DHX36-specific motif (DSM) form folds into a DNA-binding-induced alpha-helix that together with the oligonucleotide and ... Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:16150737, PubMed:18854321, PubMed: ...
... and functional aspects of DNA and RNA research. ... Mouse WRN Helicase Domain Is Not Required for Spontaneous ... To study the role of WRN helicase on spontaneous homologous recombination, we obtained a mouse with a deleted WRN helicase ... WRN is the gene mutated in WS, and is one of five human RecQ helicase family members. WS cells exhibit genomic instability and ... In this paper, we demonstrate that WRN helicase is not necessary for suppressing homologous recombination in vivo contrary to ...
Human DNA helicase II (HDH2) is ATP-dependent DNA unwinding enzyme purified from HeLa cells (Tuteja et al., 1990). HDH2 was ... Characterization of the HeLa cell single-stranded DNA-dependent ATPase/DNA helicase II. Mol Cell Biochem 146:121-126. Google ... Each of four informative peptide sequences was highly homologous to that of an 86-kD subunit of human DNA helicase II (p86) ( ... Here we produced a monoclonal antibody (termed as mH2) which specifically recognized an 86-kD subunit of Human DNA helicase II ...
Browse our DNA helicase HEL308 product catalog backed by our Guarantee+. ... DNA helicase HEL308 products available through Novus Biologicals. ... Diseases related to DNA helicase HEL308. Discover more about diseases related to DNA helicase HEL308.. Neoplasms. Nerve ... Bioinformatics Tool for DNA helicase HEL308. Discover related pathways, diseases and genes to DNA helicase HEL308. Need help? ...
Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA ... Stimulates 5-single-stranded DNA flap endonuclease activity of FEN1 in an ATP- and helicase-independent manner. Plays also a ... including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis. ... Associates with chromatin at DNA replication fork regions. Binds to single- and double-stranded DNAs (By similarity). ...
Molecular model of a helicase molecule from the SV40 virus. Helicases are enzymes that separate the two strands of the DNA ... Caption: DNA helicase. Molecular model of a helicase molecule from the SV40 virus. Helicases are enzymes that separate the two ... dna helicase, enzyme, genetics, healthcare, hexamer, illustration, large t antigen, medical, medical illustration, medicine, ... strands of the DNA double helix, by breaking the hydrogen bonds between nucleotide bases. Separation of the strands is needed ...
Our work reveals that the entire var gene family is silenced on knockout of the PfRecQ1 DNA helicase. Our study shows that ... Here, we determined how expression of the var gene family is regulated by two RecQ DNA helicase family members, PfRecQ1 and ... DNA helicase RecQ1 regulates mutually exclusive expression of virulence genes in Plasmodium falciparum via heterochromatin ... DNA helicase RecQ1 regulates mutually exclusive expression of virulence genes in Plasmodium falciparum via heterochromatin ...
Drosophila Fancm is a 3′ to 5′ DNA helicase dependent on ATP hydrolysis. (A) Schematic of Fancm. Domains and motifs present in ... Biochemical Activities and Genetic Functions of the Drosophila melanogaster Fancm Helicase in DNA Repair. Noelle-Erin Romero, ... Biochemical Activities and Genetic Functions of the Drosophila melanogaster Fancm Helicase in DNA Repair. Noelle-Erin Romero, ... Biochemical Activities and Genetic Functions of the Drosophila melanogaster Fancm Helicase in DNA Repair. Noelle-Erin Romero, ...
... their embryonic fibroblasts are sensitive to DNA damage. In tumor cells, silencing the expression of RECQL1 or WRN helicase by ... which may help development of anticancer drugs free from adverse effects by targeting DNA repair helicases RECQL1 and WRN. As ... their embryonic fibroblasts are sensitive to DNA damage. In tumor cells, silencing the expression of RECQL1 or WRN helicase by ... which may help development of anticancer drugs free from adverse effects by targeting DNA repair helicases RECQL1 and WRN. As ...
DNA helicase activity Source: UniProtKB-EC. *double-stranded DNA binding Source: dictyBase ,p>Inferred from Sequence or ... ATP-dependent DNA helicase ku70Add BLAST. 909. Proteomic databases. PaxDb, a database of protein abundance averages across all ... ATP-dependent DNA helicase ku70 (EC:3.6.4.12*Search proteins in UniProtKB for this EC number. ... "DNA-PKcs-dependent signaling of DNA damage in Dictyostelium discoideum.". Hudson J.J.R., Hsu D.-W., Guo K., Zhukovskaya N., Liu ...
... Aaron P. Landry and Huangen Ding ... Human telomere length regulator Rtel1 is a superfamily II DNA helicase and is essential for maintaining proper length of ... indicating that reactive oxygen/nitrogen species may modulate the DNA helicase activity of Rtel1 via modification of its iron- ... Purified RtelN retains a weak binding affinity for the single-stranded (ss) and double-stranded (ds) DNA in vitro. However, ...
... coli RecQ helicase. Our data show that the HRDC domain suppresses the rate of DNA-activated ATPase activity in parallel with ... are thought to be controlled by accessory DNA-binding elements including the helicase-and-RnaseD-C-terminal (HRDC) domain. The ... DNA and/or unwinding of double-stranded (ds)DNA. Here, we determined how these activities are affected by the abolition of the ... DNA-restructuring activities of RecQ-family helicases play key roles in genome maintenance. These activities, driven by two ...
DNA replication origins serve as sites of replicative helicase loading. In all eukaryotes, the six-subunit origin recognition ... the core Mcm2-7 helicase onto DNA. A key intermediate is the ORC-Cdc6-Cdt1-Mcm2-7 (OCCM) complex in which DNA has been already ... Structural mechanism of helicase loading onto replication origin DNA by ORC-Cdc6 Proc Natl Acad Sci U S A. 2020 Jul 28;117(30): ... DNA replication origins serve as sites of replicative helicase loading. In all eukaryotes, the six-subunit origin recognition ...
... chromodomain helicase DNA binding protein 6), Authors: Melissa Lathrop, C Harker Rhodes, Steve Fiering. Published in: Atlas ... BRK_domain BRK_sf Chromo-like_dom_sf Chromo/chromo_shadow_dom Chromo_domain DNA/RNA_helicase_DEAH_CS Helicase_ATP-bd Helicase_C ... transcription cofactor binding DNA binding DNA helicase activity ATP binding nucleoplasm chromatin organization DNA-dependent ... transcription cofactor binding DNA binding DNA helicase activity ATP binding nucleoplasm chromatin organization DNA-dependent ...
... chromodomain helicase DNA binding protein 5), Authors: Garrett M Brodeur, Peter S White. Published in: Atlas Genet Cytogenet ... CHD5 CHD_C2 CHD_N Chromo-like_dom_sf Chromo/chromo_shadow_dom Chromo_domain DNA/RNA_helicase_DEAH_CS DUF1086 DUF1087 Helicase_ ... heterochromatin DNA binding DNA helicase activity ATP binding nucleus nucleoplasm cytosol negative regulation of cell ... heterochromatin DNA binding DNA helicase activity ATP binding nucleus nucleoplasm cytosol negative regulation of cell ...
DNA helicases do not interact with DNA in a sequence-specific manner. Yet fusion of the TraI helicase to an N-terminal DNA ... DNA unwinding by Escherichia coli DNA helicase I (TraI) provides evidence for a processive monomeric molecular motor. J. Biol. ... DNA helicases are powerful enzymes that convert the energy of nucleoside triphosphate hydrolysis to directional DNA strand ... Concomitant reconstitution of TraI-catalyzed DNA transesterase and DNA helicase activity in vitro. J. Biol. Chem. 279:45477- ...
BLM plays a role in a number of cellular processes including DNA double-strand... ... The BLM helicase is a member of the RecQ DNA helicase family and is mutated in the cancer-prone disorder Bloom syndrome. ... The BLM helicase is a member of the RecQ DNA helicase family and is mutated in the cancer-prone disorder Bloom syndrome. BLM ... In Vivo Analysis of Drosophila BLM Helicase Function During DNA Double-Strand Gap Repair. In: Abdelhaleem M. (eds) Helicases. ...
The kinetic mechanism by which the DNA repair helicase UvrD of Escherichia coli unwinds duplex DNA was examined with the use of ... Kinetic Measurement of the Step Size of DNA Unwinding by Escherichia coli UvrD Helicase ... Kinetic Measurement of the Step Size of DNA Unwinding by Escherichia coli UvrD Helicase ... Kinetic Measurement of the Step Size of DNA Unwinding by Escherichia coli UvrD Helicase ...
In ion-exchange chromatography, both DNA helicase activity and single-stranded DNA-dependent ATPase activity were co-eluted ... The data suggest that the helicase migrates on single-stranded DNA in a 3-5 direction. These results suggest that the hRECQL4 ... The requirements of ATP and Mg for the helicase activity were different from those for the ATPase activity. ...
  • This was achieved by measuring the accumulation of fluorescent single-stranded DNA-binding protein on the single-stranded DNA product of the helicase, using total internal reflection fluorescence microscopy. (nih.gov)
  • We discuss a model, based on the 3′-to-5′ helicase activity of RecQ, to explain the role of this protein as a suppressor of illegitimate recombination. (pnas.org)
  • We offer DNA helicase HEL308 Peptides and DNA helicase HEL308 Proteins for use in common research applications: ELISA, Protein Array, Western Blot. (novusbio.com)
  • Each DNA helicase HEL308 Peptide and DNA helicase HEL308 Protein is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • The WHD mediates protein-DNA and protein-protein interactions and has been shown to facilitate DNA unwinding and contribute to substrate recognition 10 . (nature.com)
  • The functional domains of the chromodomain-helicase-DNA binding protein 5 (CHD5) are shown above. (atlasgeneticsoncology.org)
  • The central portion of the protein includes a predicted DEAH-box type helicase domain (703-999) and a putative SNF2 domain (1054-1138). (atlasgeneticsoncology.org)
  • The magnitude of stimulation depended on the proximity of the specific protein binding sites to the position of open DNA. (asm.org)
  • The cytoplasmic domain of the R1 coupling protein, TraDΔN130, stimulated helicase efficiency on all substrates in a manner consistent with cooperative interaction and sequence-independent DNA binding. (asm.org)
  • Given that transesterase and helicase domains are physically tethered in the wild-type protein, this observation suggests that an intramolecular switch controls helicase activation. (asm.org)
  • The data support a model where protein-protein and DNA ligand interactions at the coupling protein interface coordinate the transition initiating production and uptake of the nucleoprotein secretion substrate. (asm.org)
  • The protein machineries involved include nucleic acid helicases. (asm.org)
  • In ion-exchange chromatography, both DNA helicase activity and single-stranded DNA-dependent ATPase activity were co-eluted with hRECQL4 protein. (scialert.net)
  • These results suggest that the hRECQL4 protein exhibits DNA helicase activity. (scialert.net)
  • Disease variants of the human mitochondrial DNA helicase encoded by C10orf2 differentially alter protein stability, nucleotide hydrolysis and helicase activity. (thefreedictionary.com)
  • The assay can be used for any enzyme class that produces ADP, including protein, lipid or carbohydrate kinases, ATPases, DNA helicases , and carboxyltransferases. (thefreedictionary.com)
  • In a paper published in today's issue of the journal eLife , researchers explain how the helicase loader protein (P loader) from a bacterial virus attaches to the replicative helicase causing it to spiral open and quickly reclose around one of the DNA strands. (cuny.edu)
  • Roca, A. I. and Cox, M. M. (1997) RecA protein: structure, function, and role in recombinational DNA repair. (springer.com)
  • Cox, M. M. and Lehman, I. R. (1982) RecA protein-promoted DNA strand exchange: Stable complexes of recA protein and single-stranded DNA formed in the presence of ATP and single-stranded DNA binding protein. (springer.com)
  • West, S. C., Cassuto, E., and Howard-Flanders, P. (1981) RecA protein promotes homologous-pairing and strand-exchange reactions between duplex DNA molecules. (springer.com)
  • This gene encodes a protein that contains several helicase family domains. (creative-biogene.com)
  • The CHD (Chromodomain Helicase DNA Binding Protein) family of proteins is known to be involved in the regulation of gene expression, recombination and chromatin remodeling via their chromatin specific interactions and activities. (tennessee.edu)
  • Collectively, our findings suggest that CHD2 is a multi-functional protein working with the paraspeckle protein complex to facilitate both the pre-mRNA splicing process and the initial DNA repair process. (tennessee.edu)
  • The authors determine the structure of an XPD protein complexed with a short DNA fragment, and then perform mutational and biochemical analysis of XPD to define details of the XPD-ssDNA interaction. (moleculardevices.com)
  • Thus, the new allele encodes an Srs2 protein bearing only the activity of the DNA helicase. (asm.org)
  • The Srs2 protein exhibits single-stranded DNA (ssDNA)-dependent ATPase activity that unwinds DNA with 3′→5′ polarity with a k cat of ≥3,000 min −1 ( 10 , 11 ), and the Walker A motif is absolutely required for both ATPase and helicase activities ( 12 ). (asm.org)
  • DNA sequence analysis reveals that the putative Sgs1 protein is highly homologous to the helicase encoded by the Escherichia coli recQ gene. (asm.org)
  • By comparing the DNA-binding properties of a dimerization-defective protein to those of the wild type, we provide evidence that dimerization of the HPV11 DBD occurs only on two appropriately positioned E1 binding-sites and contributes approximately a 10-fold increase in binding affinity. (asm.org)
  • Finally, we show that the amino acid substitution that prevents dimerization reduces the ability of a longer E1 protein to bind to the origin in vitro and to support transient HPV DNA replication in vivo, but has little effect on its ATPase activity or ability to oligomerize into hexamers. (asm.org)
  • As an initiator protein E1 acts both as a DNA binding protein to recognize the viral origin of DNA replication and subsequently as a helicase to unwind the origin and the DNA ahead of the replication fork ( 22 , 31 , 49 ). (asm.org)
  • This abnormal chromosome segregation in srs2 cells accompanies the formation of a novel DNA damage induced during late meiotic prophase I. The damage may contain long stretches of single-stranded DNAs (ssDNAs), which lead to aggregate formation of a ssDNA binding protein, RPA, and a RecA homolog, Rad51, as well as other recombination proteins inside of the nuclei, but not that of a meiosis-specific Dmc1. (springer.com)
  • We propose that Srs2 helicase resolves early protein-DNA recombination intermediates to suppress the formation of aberrant lethal DNA damage during late prophase I. (springer.com)
  • The E coli rho transcription termination protein is a hexameric helicase, and is believed to function by separating an RNA-DNA hybrid in an ATP-dependent manner. (nii.ac.jp)
  • PIF1 5'-to-3' DNA helicase is a protein that in humans is encoded by the PIF1 gene. (wikipedia.org)
  • The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. (wikipedia.org)
  • It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. (mybiosource.com)
  • BRCA1 interacts in vivo with a novel protein, BACH1, a member of the DEAH helicase family. (semanticscholar.org)
  • We propose that the Cas3 ATPase/helicase domain acts as a motor protein, which assists delivery of the nuclease activity to Cascade-crRNA complex targeting foreign DNA. (embopress.org)
  • Prognostic significance of Cytokeratin 20-positive lymph node vascular endothelial growth factor A mRNA and chromodomain helicase DNA binding protein 4 in. (oncotarget.com)
  • Follow-up analysis for 60 months showed that patients with lymph node vascular endothelial growth factor A (VEGF-A) mRNA and chromodomain helicase DNA binding protein 4 (CHD4) mRNA expression higher than the median copies had significantly shorter time to recurrence than those with lower than the median copies. (oncotarget.com)
  • The protein encoded by this gene is a member of the RecQ DNA helicase family. (nih.gov)
  • The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. (nih.gov)
  • Plasmodium falciparum helicase 45 (PfH45) is a putative Superfamily 2 (SF2) nucleic acid helicase, predicted to be a 45 kDa monomeric protein with two distinct domains. (port.ac.uk)
  • This work was undertaken between 2009-2012 by Dr Luke Evans and Dr James Youell, and data on the codon optimisation, gene cloning, protein purification and helicase-unwinding assays has been published (Evans et al, 2012, Protein Expr. (port.ac.uk)
  • A recent PhD student (Dr Claire Fraser) has completed a series of studies looking at the importance of AMP-containing cofactors, substrate order-of-addition studies, and length-dependency and processivity studies (unpublished) to look for evidence of facilitated diffusion and 1D vs. 3D protein motion on nicked DNA. (port.ac.uk)
  • We demonstrate that RecQ helicase from Escherichia coli is a catalytic helicase whose activity depends on the concentration of ATP, free magnesium ion, and singlestranded DNA-binding (SSB) protein. (elsevier.com)
  • Omitting Escherichia coli SSB protein lowers the rate and extent of dsDNA unwinding, suggesting that RecQ helicase associates with the single-stranded DNA (ssDNA) product. (elsevier.com)
  • Thus, we conclude that SSB protein stimulates RecQ helicase-mediated unwinding by both trapping the separated ssDNA strands after unwinding and preventing the formation of non-productive enzyme-ssDNA complexes. (elsevier.com)
  • A large part of DNA (more than 98% for humans) is non-coding , meaning that these sections do not serve as patterns for protein sequences . (wikipedia.org)
  • The RNA helicase, nucleotide 5′-triphosphatase, and RNA 5′-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic core. (springer.com)
  • The interdomain region of dengue NS5 protein that binds to the viral helicase NS3 contains independently functional importin beta 1 and importin alpha/beta-recognized nuclear localization signals. (springer.com)
  • Chromodomain-helicase-DNA-binding protein 9 is an enzyme that in humans is encoded by the CHD9 gene. (creative-biogene.com)
  • When NS3 was passed through a small pore filter capable of removing protein aggregates, greater than 95% of the protein and the DNA unwinding activity was removed from solution. (elsevier.com)
  • Junk DNA is non-coding DNA it does not code for protein. (answers.com)
  • The relaxase of the conjugative F plasmid is part of the large multifunctional TraI protein that also contains highly efficient helicase and putative protein-binding C-terminal domains. (grantome.com)
  • The E1 protein from bovine papillomavirus has site-specific DNA binding activity, DNA helicase activity, and DNA-dependent ATPase activity consistent with the properties of an initiator protein. (cshl.edu)
  • The Bloom syndrome (BS) protein, BLM, is a member of the RecQ DNA helicase family that also includes the Werner syndrome protein, WRN. (elsevier.com)
  • Strikingly, human replication protein A (hRPA) not only ensures that limited quantities of BLMs unwind nicked dsDNA under a reduced force but also permits the translocation of BLMs on both intact and nicked single-stranded DNA (ssDNA), resulting in a bidirectional unwinding mode (Figure 1B). (champlainresources.com)
  • DNA helicases, members of which are found in each of the SF, are an essential group of motor proteins that unwind DNA duplexes into their component single strands in a process that is coupled to the hydrolysis of nucleoside 5′‐triphosphates (NTPs). (els.net)
  • Bacterial rep proteins, a single-stranded DNA-dependent ATPase involved in DNA replication which can initiate unwinding at a nick in the DNA. (ebi.ac.uk)
  • Fungal srs2 proteins, an ATP-dependent DNA helicase involved in DNA repair. (ebi.ac.uk)
  • DNA helicases are motor proteins that catalyze the unwinding of double-stranded DNA into single-stranded DNA using the free energy from ATP hydrolysis. (nih.gov)
  • Our DNA helicase HEL308 Peptides and DNA helicase HEL308 Proteins can be used in a variety of model species: Human. (novusbio.com)
  • SNF2 domains are commonly observed in, although not restricted to, proteins involved in chromatin unwinding, DNA repair and recombination, and transcriptional regulation. (atlasgeneticsoncology.org)
  • Linear heteroduplex substrates containing double-stranded DNA binding sites for plasmid R1 relaxosome proteins and various regions of open duplex for TraI helicase loading were constructed to model putative intermediate structures in the initiation pathway. (asm.org)
  • Genome-manipulating and -regulating proteins are central to that biological function in recognizing appropriate DNA targets at initiation sequences and unwinding the complementary strands to provide single-stranded DNA (ssDNA) templates for nucleic acid synthesis and other processing reactions. (asm.org)
  • At replication initiation helicases gain controlled access to the double-stranded genome at positions determined by the DNA binding properties of initiator proteins that comprise an origin recognition complex ( 1 , 9 , 17 , 31 , 45 , 66 ). (asm.org)
  • We believe this represents a novel mutator mode-of-action and opens up new prospects to understand how upregulation of DNA repair proteins may contribute to mutagenesis. (genetics.org)
  • Until recently, however, researchers have not understood how the helicase-a donut-shaped enzyme composed of six identical proteins-is able to thread just one of the strands when they are bound together. (cuny.edu)
  • Caption: Loader proteins (orange) attach to the DnaB replicative helicase (white), causing it to briefly spiral open. (cuny.edu)
  • Through our research we were able to identify the mechanism for loading the DNA strand into the helicase, and we also learned that the loader proteins prevent any movement of the helicase at the moment it opens and closes in order to prevent any replication mistakes. (cuny.edu)
  • They employed cryo electron microscopy and tomography to image the helicase and all of its loader proteins. (cuny.edu)
  • Kowalczykowski, S. C. and Eggleston, A. K. (1994) Homologous pairing and DNA strand-exchange proteins. (springer.com)
  • Harmon, F. G. and Kowalczykowski, S. C. (1998) RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination. (springer.com)
  • Helicases are ubiquitous motor proteins that move along nucleic acids and separate duplex DNA or RNA into its component strands. (elifesciences.org)
  • Additionally, mass spectrometric analysis showed possible association of CHD2 with the paraspeckle family of proteins known to be involved in an array of cellular processes specifically in RNA processing and DNA repair. (tennessee.edu)
  • Maintenance of the viral genome in infected cells requires the activity of E1 and E2, the two viral proteins necessary for replication of the HPV genome in conjunction with the host cell DNA replication machinery (reviewed in reference 12 ). (asm.org)
  • In thermophilic archaea, the HerA helicase and NurA nuclease cooperate with the highly conserved Mre11 and Rad50 proteins during HR-dependent DNA repair. (lancs.ac.uk)
  • Chromodomain helicase DNA-binding (CHD) proteins is a subfamily of ATP-dependent chromatin remodeling complexes (remodelers). (wikipedia.org)
  • 100 µg) Chd1 belongs to a family of proteins that is characterized by the presence of chromo domains and SNF2-related helicase/ATPase domains. (allelebiotech.com)
  • Functions and regulation of the multitasking FANCM family of DNA motor proteins. (semanticscholar.org)
  • The group is particularly interested in addressing how these site-specific proteins initially locate target sequences that are buried amongst large stretches of non-target DNA. (port.ac.uk)
  • The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. (worktribe.com)
  • RNA strands are created using DNA strands as a template in a process called transcription , where DNA bases are exchanged for their corresponding bases except in the case of thymine (T), which RNA substitutes for uracil (U). [4] Under the genetic code , these RNA strands specify the sequence of amino acids within proteins in a process called translation . (wikipedia.org)
  • Within eukaryotic chromosomes, chromatin proteins, such as histones , compact and organize DNA. (wikipedia.org)
  • These compacting structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed. (wikipedia.org)
  • A key class of proteins involved in various DNA repair pathways is comprised of energydriven nucleic acid unwinding enzymes known as helicases. (eurekaselect.com)
  • The difference between a chromosome and a DNA molecule is that a chromosome is a single DNA molecule associated with proteins and a DNA molecule is a long thin molecule that stores genetic information. (answers.com)
  • DNA helicases are proteins, while DNA molecules are nucleotides. (answers.com)
  • The XPD and FancJ proteins are related helicases involved in the nucleotide excision repair (NER) and Fanconi anemia repair pathways, respectively. (st-andrews.ac.uk)
  • Among them, the RecQ family helicases are a kind of proteins that are very conserved in the evolutionary process. (champlainresources.com)
  • BLM helicase works with multiple proteins, it processes distinct DNA substrates, but not nicked DNA, during many steps in DNA replication and repair. (champlainresources.com)
  • Helicases and translocases are proteins that use the energy derived from ATP hydrolysis to move along or pump nucleic acid substrates. (ens.fr)
  • DNA helicases participate in every aspect of DNA metabolism due to the requirement for transient separation of small regions of the duplex genome into its component strands so that replication, recombination and repair can occur. (els.net)
  • Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. (ebi.ac.uk)
  • The Escherichia coli recQ gene encodes a DNA helicase with an activity that unwinds duplex DNA in the 3′-to-5′ direction ( 1 ), and recQ is known to play an important role in homologous recombination by the RecE and RecF pathway ( 2 , 3 ). (pnas.org)
  • However, more recent studies propose that other RecQ helicases (including WRN) promote early events of homologous recombination. (hindawi.com)
  • To study the role of WRN helicase on spontaneous homologous recombination, we obtained a mouse with a deleted WRN helicase domain and combined it with the in vivo pink-eyed unstable homologous recombination system. (hindawi.com)
  • In this paper, we demonstrate that WRN helicase is not necessary for suppressing homologous recombination in vivo contrary to previous reports using a similar mouse model. (hindawi.com)
  • RECQL1 and WRN helicases in the human RecQ helicase family participate in maintaining genome stability, DNA repair, replication, and recombination pathways in the cell cycle. (frontiersin.org)
  • The DNA repair system contains various reactions, such as nucleotide excision repair, base excision repair, homologous recombination (HR), and non-homologous end joining (NHEJ) recombination pathways, mismatch repair pathways, and telomere maintenance for genomic integrity, in all of which RecQ helicases are considered to participate. (frontiersin.org)
  • By recovering and molecularly analyzing individual repair products from wild-type and mus309 male pre-meiotic germline cells, we demonstrated that the DmBlm helicase is involved in homologous recombination downstream of strand invasion. (springer.com)
  • Wu L. and Hickson I. D. (2003) The Bloom's syndrome helicase suppresses crossing over during homologous recombination. (springer.com)
  • A gene on chromosome 15q26.1 that encodes a DExH box-containing DNA helicase with both DNA-stimulated ATPase and ATP-dependent DNA helicase activities, which is thought to suppress inappropriate recombination events. (thefreedictionary.com)
  • top3 deletion is inviable, and this is suppressed by concomitant loss of rqh1 helicase activity or loss of recombination functions. (thebiogrid.org)
  • Histone modifying enzymes and chromatin remodeling complexes play an important regulatory role in chromatin dynamics that dictate the interaction of regulatory factors involved in processes such as DNA replication, recombination, repair and transcription, with DNA template. (tennessee.edu)
  • Homologous recombination (HR) is a mechanism that repairs a variety of DNA lesions. (asm.org)
  • IMPORTANCE Homologous recombination (HR) is a key mechanism that repairs damaged DNA. (asm.org)
  • Christman MF, Dietrich FS, Fink GR. Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II. (pubmedcentralcanada.ca)
  • Foury F, Lahaye A. Cloning and sequencing of the PIF gene involved in repair and recombination of yeast mitochondrial DNA. (pubmedcentralcanada.ca)
  • A diverged chromosomal substrate was used as a repair template for the gapped plasmid, allowing mismatch-containing heteroduplex DNA (hDNA) formed during recombination to be monitored. (prolekare.cz)
  • To prevent these outcomes, DSBs are repaired by one of two pathways: error-prone nonhomologous end joining or error-free homologous recombination (HR). As the major DSB repair pathway in the yeast Saccharomyces cerevisiae , HR promotes high-fidelity repair through the use of an intact template DNA sequence. (prolekare.cz)
  • In this study, we found that the deletion of the SRS2 gene, which encodes a DNA helicase necessary for the control of homologous recombination, induces aberrant chromosome segregation during budding yeast meiosis. (springer.com)
  • Helicase-nuclease systems dedicated to DNA end resection in preparation for homologous recombination (HR) are present in all kingdoms of life. (lancs.ac.uk)
  • The T4 bacteriophage Dda helicase is believed to be involved in early events in T4 DNA replication and has been shown to stimulate genetic recombination processes in vitro. (elsevier.com)
  • The error-free repair of double-strand DNA breaks by homologous recombination (HR) ensures genomic stability using undamaged homologous sequence to copy genetic information. (semanticscholar.org)
  • Reconstituted System for the Examination of Repair DNA Synthesis in Homologous Recombination. (semanticscholar.org)
  • Critical role in recombination and DNA repair. (cusabio.com)
  • RecQ helicase functions in both early and late stages of homologous recombination and unwinds DNAs with particular structures, such as the fork structure for replication, the Holliday junction which is an intermediate of recombination, telomere, quadruplex G4-DNA, etc. (antibody-antibodies.com)
  • Studies on mechanisms of DNA recombination, replication and repair. (antibody-antibodies.com)
  • The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. (cusabio.com)
  • In vitro studies showed that defects in RECQL4 impair homologous recombination, sensitizing BC cells to DNA-damaging agents. (nih.gov)
  • In addition to DNA repair, helicases have been implicated in the cellular processes of replication, recombination, transcription, and RNA stability/processing. (eurekaselect.com)
  • Component of the MCM8 - MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). (rcsb.org)
  • Dual nuclease and helicase activities of Helicobacter pylori AddAB are required for DNA repair, recombination, and mouse infectivity. (fredhutch.org)
  • E. coli DeltarecBCD expressing H. pylori addAB was recombination-deficient unless H. pylori recA was also expressed, suggesting a species-specific interaction between AddAB and RecA and also that H. pylori AddAB participates in both DNA repair and recombination. (fredhutch.org)
  • In budding and fission yeasts, Pif1 family helicases impact both telomerase-mediated and semi-conservative replication of telomeric DNA as well as recombination-mediated telomere lengthening. (princeton.edu)
  • DNA helicases are essential components of the cellular machinery for DNA replication, recombination, repair, and transcription. (st-andrews.ac.uk)
  • They play important roles in maintaining the stability of the genome by participating in many cellular processes, such as DNA replication, recombination, and repair. (champlainresources.com)
  • Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination: promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed:17724085, PubMed:19736316). (nih.gov)
  • DNA helicase II [Escherichia coli str. (nih.gov)
  • Microorganisms, such as Escherichia coli and Saccharomyces cerevisiae , contain only one RecQ helicase, but higher eukaryotes have more than one RecQ helicase. (frontiersin.org)
  • The kinetic mechanism by which the DNA repair helicase UvrD of Escherichia coli unwinds duplex DNA was examined with the use of a series of oligodeoxynucleotides with duplex regions ranging from 10 to 40 base pairs. (sciencemag.org)
  • Sargentini, N. J. and Smith, K. C. (1986) Characterization and quantitation of DNA strand breaks requiring recA-dependent repair in X-irradiated Escherichia coli . (springer.com)
  • Roman, L. J. and Kowalczykowski, S. C. (1989) Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay. (springer.com)
  • Here, we developed a computational technique combining structural bioinformatics approaches and atomic-level free-energy simulations to characterize how the Escherichia coli DNA repair enzyme UvrD changes its conformation at the fork junction to switch its function from unwinding to rezipping DNA. (elifesciences.org)
  • Harmon, FG & Kowalczykowski, SC 2001, ' Biochemical characterization of the DNA helicase activity of the Escherichia coli RecQ helicase ', Journal of Biological Chemistry , vol. 276, no. 1, pp. 232-243. (elsevier.com)
  • A review was done to compare the mechanistic features of Escherichia coli Rep helicase and kinesin, which both function as homodimeric enzymes. (readabstracts.com)
  • Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) helicases are organised into six SuperFamilies (SF) of enzymes using sequence alignments, biochemical data and available crystal structures. (els.net)
  • These enzymes share common biochemical properties that include the binding of single‐ and double‐stranded DNA, NTP binding and hydrolysis, and NTP hydrolysis‐coupled, polar unwinding of duplex DNA. (els.net)
  • Single molecule approaches enable us to address detailed mechanistic questions about how such enzymes move processively along DNA. (nih.gov)
  • By immobilizing either the DNA or helicase, localized increase in fluorescence provides information about the rate of unwinding and the processivity of individual enzymes. (nih.gov)
  • The method is an important addition to the single-molecule toolbox available for studying DNA processing enzymes. (nih.gov)
  • Helicases are enzymes that separate the two strands of the DNA double helix, by breaking the hydrogen bonds between nucleotide bases. (sciencephoto.com)
  • The capability of RecQ helicases to process complex DNA structures is associated with a conserved domain architecture shared by Ec RecQ, human BLM and WRN enzymes, as well as at least one RecQ homolog in almost all investigated organisms ( Fig. 1 ) 10 . (nature.com)
  • These enzymes all contain a highly conserved HELICc DEAD-like helicase/ATPase with seven conserved catalytic regions (Brown et al. (atlasgeneticsoncology.org)
  • DNA helicases are powerful enzymes that convert the energy of nucleoside triphosphate hydrolysis to directional DNA strand translocation and separation of the double helix into its constituent single strands (for reviews, see references 13 , 14 , 16 , 38 , 55 , and 64 ). (asm.org)
  • By necessity, these enzymes interact with DNA strands via mechanisms independent of sequence recognition. (asm.org)
  • These enzymes are remarkable in that the same polypeptides additionally harbor in a distinct domain a DNA transesterase activity. (asm.org)
  • Instead, in vivo DNA replication methods for most organisms rely on a class of enzymes known as DNA helicases , which act to locally unwind dsDNA to create small ssDNA regions suitable for polymerase activity. (thefreedictionary.com)
  • Recombinational DNA repair is one such pathway, and it requires the coordinated action of many different enzymes. (springer.com)
  • Helicases play key roles in genome maintenance, yet it remains elusive how these enzymes change conformations and how transitions between different conformational states regulate nucleic acid reshaping. (elifesciences.org)
  • Mutations in genes encoding DNA helicases of the RecQ and iron-sulfur (Fe-S) helicase families are linked to hereditary disorders characterized by chromosomal instabilities, highlighting the importance of these enzymes. (biochemsoctrans.org)
  • Helicases are a class of enzymes that utilize the energy of ATP hydrolysis to promote unwinding of 1. (semanticscholar.org)
  • In the past, research on helicases mainly focused on the mechanism of individual enzymes. (biochemistry.org)
  • DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. (nih.gov)
  • PROJECT NARRATIVE Conjugative DNA transfer, the primary route by which antibiotic resistance genes spread through bacterial populations, is initiated and driven by DNA relaxase and helicase enzymes. (grantome.com)
  • DNA helicases are a class of enzymes vital to all organisms. (champlainresources.com)
  • Bacterial helicase IV (helD gene product). (ebi.ac.uk)
  • The Bam HI- Eco RI DNA fragment containing the recQ + gene was prepared by PCR with the oligonucleotides 5′-CCGGATCCATGAACGGTTGAGTGGTTGG-3′ and 5′-CCGAATTCACATACATTTGACTCGCGGG-3′ as primers, chromosomal DNA of an E. coli strain C600 as a template, and TaKaRa Ex Taq (Takarashuzo, Kyoto, Japan) as a polymerase. (pnas.org)
  • WRN is the gene mutated in WS, and is one of five human RecQ helicase family members. (hindawi.com)
  • 1995) The Bloom's syndrome gene product is homologous to RecQ helicases. (springer.com)
  • Mutations in any one of these genes, such as in the DNA helicase gene associated with Werner's syndrome, may trigger major portions of the aging phenotype. (thefreedictionary.com)
  • These patients are homozygous for the BLM gene on the long arm of chromosome 15, which has also been recently shown to encode a DNA helicase [49]. (thefreedictionary.com)
  • This review will discuss and contrast the specialized molecular functions and biological roles of RecQ and Fe-S helicases in DNA repair, the replication stress response, and the regulation of gene expression, laying a foundation for continued research in these important areas of study. (biochemsoctrans.org)
  • This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. (wikipedia.org)
  • deacetylation of the histone residue H3K9ac is an example of how the NuRD complex can downregulate gene expression and affect DNA topology. (wikipedia.org)
  • This looks like it will be totally usable as a standard curve for developing a qPCR assay that targets the RLOv DNA helicase gene. (onsnetwork.org)
  • ATP-dependent DNA helicase gene ( PfRuvB3 ) of Plasmodium falciparum strain K1, a chloroquine and pyrimethamine-resistant strain, was inserted into pQE-TriSystem His-Strep 2 vector, heterologously expressed and affinity purified. (beds.ac.uk)
  • Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities. (nih.gov)
  • DNA-restructuring activities of RecQ-family helicases play key roles in genome maintenance. (nature.com)
  • 2007) Multiple functions of Drosophila BLM helicase in maintenance of genome stability. (springer.com)
  • To prevent replication fork collapse and genome instability under replicative stress, DNA damage tolerance (DDT) mechanisms have evolved. (mdpi.com)
  • The relative importance of Srs2's helicase activity, Rad51 removal function, and PCNA interaction in genome stability remains unclear. (asm.org)
  • The relative roles of the helicase and Rad51 removal activities of Srs2 in genome stability remain unclear. (asm.org)
  • The E1 helicase of papillomaviruses is required for replication of the viral double-stranded DNA genome, in conjunction with cellular factors. (asm.org)
  • These viruses infect the basal cell layer where they establish their small double-stranded DNA genome, 7.9 kbp in length, as a circular extrachromosomal element in the nucleus of infected cells. (asm.org)
  • Recent developments such as the identification of target sites and interaction partners on a genome-wide level, and advances in structural biology and single molecule imaging in vitro and in vivo with increasing resolution, now increasingly allow to understand the regulation of helicase function within large complexes and regulatory networks in their natural environment. (biochemistry.org)
  • DNA polymerase blockage leads to replication arrest and can gives rise to genome instability. (frontiersin.org)
  • Here we review human disorders that are caused by defects in genome stability to explore the role of DNA damage in aging and disease. (frontiersin.org)
  • It was estimated that DNA damage occurs on the order of tens of thousands per genome on a daily basis ( Lindahl and Nyberg, 1972 ). (frontiersin.org)
  • The difference between Eukaryotic DNA and bacterial genome replication is the eukaryotic DNA is mostly linear and has multiple sites of replication. (answers.com)
  • The budding yeast genome encodes two Pif1 family helicases, Pif1 and Rrm3, previously shown to have distinct functions in the maintenance of telomeres and other aspects of genome stability. (nature.com)
  • Genome-wide analyses of S. cerevisiae replisome mobility reveal overlapping roles of Pif1 and Rrm3 helicases in alleviating replication-fork arrest at tRNA genes. (nature.com)
  • It unwinds DNA duplexes with 3'-5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. (ebi.ac.uk)
  • Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:16150737, PubMed:18854321, PubMed:20472641, PubMed:21586581). (rcsb.org)
  • Unwinds a G4-RNA structure located in the 3'-UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (PubMed:27940037). (rcsb.org)
  • C) Fancm unwinds duplex DNA. (genetics.org)
  • Analysis of these kinetics indicates that UvrD unwinds duplex DNA in discrete steps, with an average "step size" of 4 to 5 base pairs (approximately one-half turn of the DNA helix). (sciencemag.org)
  • G40P unwinds DNA in one base pair steps und slips backwards. (elifesciences.org)
  • The helicase briefly stalls at a characteristic FRET efficiency or slips after stalling and unwinds in a second attempt. (elifesciences.org)
  • The helicase rarely unwinds three consecutive GC base pairs completely (top trace). (elifesciences.org)
  • Dda unwinds double-stranded DNA with 5' to 3' polarity but its ability to translocate on DNA has not been established. (elsevier.com)
  • The direction of translocation on ssDNA is consistent with the direction in which Dda unwinds duplex DNA and is not dependent on duplex structure. (elsevier.com)
  • Unwinds branched duplex DNA (Y-DNA). (cusabio.com)
  • Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. (nih.gov)
  • Unwinds double-stranded DNA and RNA in a 3' to 5' direction. (abcam.com)
  • RHA is a nuclear helicase that unwinds dsRNA and RNA:DNA duplexes in a reaction driven by hydrolysis of ATP ( 10 , 11 ). (jimmunol.org)
  • RHA is identical over most of its length with the independently reported DNA helicase II, which unwinds both dsRNA and dsDNA ( 12 , 13 , 14 , 15 ). (jimmunol.org)
  • The enzyme is shown as a ribbon (dark blue) bound to a tailed DNA molecule (cyan and yellow). (els.net)
  • b) The enzyme is viewed from the side bound to DNA (orange). (els.net)
  • The leading domain of RecB can be seen reaching out ahead of the body of the enzyme to pull the duplex DNA into the complex where it is unwound. (els.net)
  • In this image, the DNA is coloured black and the RecB and C subunits are transparent so that the path of the DNA through the enzyme can be easily seen. (els.net)
  • Gram-positive bacterial pcrA helicase, an essential enzyme involved in DNA repair and rolling circle replication. (ebi.ac.uk)
  • RecBCD is a multi-functional enzyme complex that processes DNA ends resulting from a double-strand break. (ebi.ac.uk)
  • Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks. (ebi.ac.uk)
  • The exposed ssDNA mediates helicase binding followed by directional translocation along that strand until the enzyme engages the duplex for unwinding. (asm.org)
  • an enzyme that catalyzes the energy-dependent unwinding of the DNA double helix during DNA replication. (thefreedictionary.com)
  • In both cases, fluoroquinolones appear to trap the enzyme on DNA during the topoisomerization reaction, forming a physical barrier to the movement of the replication fork (4), RNA polymerase (5), and DNA helicase (6). (thefreedictionary.com)
  • Taylor, A. and Smith, G. R. (1980) Unwinding and rewinding of DNA by the recBC enzyme. (springer.com)
  • Binding of ATP analog at the interface of the SF2 helicase RecA-like domains rearranges a motif V with implications for the enzyme mechanism. (nottingham.ac.uk)
  • We have recently shown that the relaxase enzyme essential to this DNA transfer process can be inhibited with nanomolar efficacy using a variety of small molecules, including some osteoporosis drugs. (grantome.com)
  • In the present report we describe a new autoantibody system that recognizes human RNA helicase A (RHA) and show for the first time that this enzyme is cleaved by caspase-3 as an early event in apoptosis. (jimmunol.org)
  • We report on four single molecule manipulation systems addressing the mechanism of different helicases using specifically designed DNA substrates: UvrD enzyme activity detection on a stretched nicked DNA molecule, HCV NS3 helicase unwinding of a RNA hairpin under tension, the observation of RecBCD helicase/nuclease forward and backward motion, and T7 gp4 helicase mediated opening of a synthetic DNA replication fork. (ens.fr)
  • Energy from ATP hydrolysis is used to translocate the hexamer along the single‐stranded DNA in a 5′ to 3′ direction (gp4 is a 5′‐to‐3′ helicase). (els.net)
  • Unwinding of a DNA helix, driven by ATP hydrolysis. (yeastgenome.org)
  • Drosophila Fancm is a 3′ to 5′ DNA helicase dependent on ATP hydrolysis. (genetics.org)
  • The HRDC domain of human Bloom's syndrome (BLM) helicase was shown to interact with the RecA core, raising the possibility that it may affect the coupling between ATP hydrolysis, translocation along single-stranded (ss)DNA and/or unwinding of double-stranded (ds)DNA. (nature.com)
  • In this ATP-driven mechanism, energy from hydrolysis causes the lobes to 'crawl' along the DNA towards the nucleosome dyad until the nucleosomes are correctly assembled, accessed or edited. (wikipedia.org)
  • Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. (mybiosource.com)
  • DEAH ATP helicases are ATP-dependent and are usually associated with nucleic acid unwinding. (atlasgeneticsoncology.org)
  • Helicases are molecular motors that play central roles in nucleic acid metabolism. (biochemsoctrans.org)
  • The HD- nucleolytic site contains two metal ions that are positioned at the end of a proposed nucleic acid-binding tunnel running through the SF2 helicase structure. (nottingham.ac.uk)
  • Helicases and nucleic-acid based machines are involved in all aspects of nucleic acid metabolism. (biochemistry.org)
  • Since 1999 this biannual meeting has developed our understanding of helicases and nucleic acid-based machines. (biochemistry.org)
  • We use these to study several nucleic-acid binding molecules, including DNA helicases, DNA ligases and DNA triple helices. (port.ac.uk)
  • Because of their essential roles in nucleic acid metabolism, and more specifically the DNA damage response, helicases may be a suitable target of chemotherapy. (eurekaselect.com)
  • Chromodomains are highlighted in purple, the highly conserved SNF2-like ATPase region is indicated in light blue, and the DNA binding domain (green oval) contains BRK binding sights, indicated by the red diamonds. (atlasgeneticsoncology.org)
  • Pontin is a highly conserved DNA helicase/ATPase which is a element of several macromolecular processes with features that include DNA fix, telomere maintenance and growth reductions. (exposed-skin-care.net)
  • RecQ helicases are highly conserved from bacteria to man. (semanticscholar.org)
  • Fancm ATPase activity was examined as a function of DNA concentration using either M13mp18 ssDNA (□⋄) or dsDNA (▪♦) as the DNA cofactor. (genetics.org)
  • Our data show that the HRDC domain suppresses the rate of DNA-activated ATPase activity in parallel with those of ssDNA translocation and dsDNA unwinding, regardless of the ssDNA binding capability of this domain. (nature.com)
  • SF1 helicases can unwind dsDNA by translocating on a ssDNA strand as shown in Figure 1a . (elifesciences.org)
  • The helicase uses chemical energy from ATP (hydrolyzed at the site labeled with a yellow ellipse between two motor domains) to unwind dsDNA. (elifesciences.org)
  • It can unwind a variety of substrates, including those containing forks, flaps, D -loops, 3′ and 5′ single-stranded DNA overhangs, blunt-end double-stranded DNA (dsDNA) substrates, and Holliday junctions ( 11 , 13 ). (asm.org)
  • Type I CRISPR-Cas systems use a "Cascade" ribonucleoprotein complex to guide RNA specifically to complementary sequence in invader double-stranded DNA (dsDNA), a process called "interference. (nottingham.ac.uk)
  • After target recogni- tion by Cascade, formation of an R-loop triggers recruitment of a Cas3 nuclease-helicase, completing the interference process by destroying the invader dsDNA. (nottingham.ac.uk)
  • Therefore, RecQ helicase utilizes multiple, interacting ATP-binding sites to mediate double-stranded DNA (dsDNA) unwinding, implicating a multimer of at least three subunits as the active unwinding species. (elsevier.com)
  • Unwinding activity is independent of dsDNA ends, indicating that RecQ helicase can unwind from both internal regions and ends of dsDNA. (elsevier.com)
  • In this study, using a combined single-molecule approach (Figure 1A), the researchers found that a high abundance of BLMs can indeed unidirectionally unwind double-stranded DNA (dsDNA) from a nick when an external destabilizing force is applied. (champlainresources.com)
  • We then discuss experiments on two dsDNA translocases: the RuvAB motor studied on its natural substrate, the Holliday junction, and the chromosome-segregation motor FtsK, showing its unusual coupling to DNA supercoiling. (ens.fr)
  • Unwinding of partial duplex DNA substrates by Fancm. (genetics.org)
  • Time-course unwinding of partial-duplex DNA substrates by Fancm. (genetics.org)
  • We found that a T-antigen hexamer complex acts during the unidirectional unwinding of appropriate DNA substrates and is localized directly in the center of the fork, contacting the adjacent double strand as well as the emerging single strands. (asm.org)
  • HCV NS3 helicase exhibits activity toward DNA and RNA substrates. (elsevier.com)
  • Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA damage. (uniprot.org)
  • 26089203 ). Associates with chromatin at DNA replication fork regions. (uniprot.org)
  • The role of simian virus 40 (SV40) large tumor antigen (T antigen) as a DNA helicase at the replication fork was studied. (asm.org)
  • Poly(dA:dT) tracts characterize strong DNA replication origins in mammals and cause replication-fork collapse and DNA breaks that underlie the expression of fragile sites. (nature.com)
  • Resolves G4-DNA structures in promoters of genes, such as YY1 , KIT /c-kit and ALPL and positively regulates their expression (PubMed:21993297). (rcsb.org)
  • Discover related pathways, diseases and genes to DNA helicase HEL308. (novusbio.com)
  • Recent data reveal that the products of these two genes, BLM and ATM, interact and function together in recognizing abnormal DNA structures. (nii.ac.jp)
  • Identification of genes that are associated with DNA repeats in prokaryotes. (semanticscholar.org)
  • E. coli RecQ helicase contributes to the genomic stability as the prototype of RecQ family DNA helicases, mutations of which genes are associated with premature aging and cancer susceptibility, known as Bloom's and Werner's syndromes (1). (antibody-antibodies.com)
  • In support of this, a growing number of human genetic disorders are attributed to mutations in helicase genes. (eurekaselect.com)
  • Here the authors identify a role for Pif1 (and Rrm3) in promoting DNA replication and suppressing R-loop mediated DNA damage at tRNA genes. (nature.com)
  • In contrast, BS cells have a normal Fas-mediated apoptosis, and in response to DNA damage normal accumulation of p53, normal induction of p53 responsive genes, and normal G 1 -S and G 2 -M cell cycle arrest. (elsevier.com)
  • RECQL1 and RECQL5 helicases, however, have no association with mutations of human disorders. (frontiersin.org)
  • Thus, the process of DNA replication associates with many errors that expose growing cells to a risk of acquiring mutations. (frontiersin.org)
  • For example, some deficiencies in DNA damage repair components will increase the spontaneous mutation rate, allowing the cells to acquire mutations that may offer a selective advantage and aid in the evolution and progression of tumors ( Hanahan and Weinberg 2011 ). (genetics.org)
  • Moreover, mono-allelic RecQ and Fe-S helicase mutations are associated with a broad spectrum of cancers. (biochemsoctrans.org)
  • The DNA of identical twins and of clones is, apart from random, environmentally produced mutations, 100% identical. (answers.com)
  • H. pylori with the single nuclease domain mutations were somewhat less sensitive to the DNA-damaging agent ciprofloxacin than the corresponding deletion mutant, suggesting that residual nuclease activity promotes limited DNA repair. (fredhutch.org)
  • Clinically relevant mutations in patients with trichothiodystrophy (TTD) and Fanconi anemia disrupt the Fe-S clusters of XPD and FancJ and thereby abolish helicase activity. (st-andrews.ac.uk)
  • Spies and Fishel, 2015 ) and nucleotide excision repair ( Sancar, 1996 ) by unwinding duplex DNA. (elifesciences.org)
  • DNA helicases have been either implicated or have proposed roles in nucleotide excision repair, mismatch repair, base excision repair, double strand break repair, and most recently cross-link repair. (eurekaselect.com)
  • The helicase (dark blue) is shown bound to a tailed DNA molecule (orange). (els.net)
  • Component of a multi-helicase- TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (By similarity). (rcsb.org)
  • Molecular model of a helicase molecule from the SV40 virus. (sciencephoto.com)
  • Replicative Dna Helicase Molecule is a photograph by Laguna Design/science Photo Library which was uploaded on September 24th, 2018. (fineartamerica.com)
  • There are no comments for Replicative Dna Helicase Molecule . (fineartamerica.com)
  • The helicase then begins running along the strand and breaking the hydrogen bounds that bind it to the second strand, allowing each to become a substrate that can replicate a complete DNA molecule. (cuny.edu)
  • These 3′ ends are coated with Rad51 to form nucleoprotein filaments that are competent to conduct a homology search and invade a donor duplex DNA molecule, promoting pairing with the complementary strand. (prolekare.cz)
  • [1] DNA ) is a molecule composed of two chains that coil around each other to form a double helix carrying genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses . (wikipedia.org)
  • The unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. (wikipedia.org)
  • The DNA helicase activity of NS3 has been proposed to be optimal when multiple NS3 molecules are bound to the same substrate molecule. (elsevier.com)
  • Here we first describe the basic mechanical properties of DNA unraveled by single molecule manipulation techniques. (ens.fr)
  • These findings indicate that RECQL1 and WRN helicases are ideal molecular targets for cancer therapy. (frontiersin.org)
  • The molecular mechanisms underlying these events has been studied extensively, which may help development of anticancer drugs free from adverse effects by targeting DNA repair helicases RECQL1 and WRN. (frontiersin.org)
  • We used molecular simulations to reveal the dynamic transition from preloading conformers to the loaded conformers in which the loading of Mcm2-7 on DNA is complete and the DNA entry gate is fully closed. (nih.gov)
  • Our work provides multiple molecular insights into a key event of eukaryotic DNA replication. (nih.gov)
  • We show that Rqh1, the single Schizosaccharomyces pombe homologue, is a 3'-to-5' helicase and exists with Top3 in a high-molecular-weight complex. (thebiogrid.org)
  • The present study deciphers key elements for the 'hyper-helicase' behavior of a mutant and provides an effective framework to characterize directly structure-function relationships in molecular machines. (elifesciences.org)
  • Therefore, Nd acts as a molecular 'switch' regulating access to the ssDNA binding site on Cd, in response to binding of the helicase. (worktribe.com)
  • DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity . (wikipedia.org)
  • This proposal focuses on extending our preliminary structural and chemical biology studies with the goals of understanding the molecular basis of DNA transfer and developing small molecules capable of killing antibiotic resistant bacteria. (grantome.com)
  • This project will extend our preliminary structural and chemical biology discoveries with the goal of understanding the molecular basis of DNA transfer and developing drugs that potently kill antibiotic resistant bacteria. (grantome.com)
  • Recombinant PfRuvB3 has molecular size of 59 kDa, showing both DNA helicase and ATPase activities. (beds.ac.uk)
  • DNA helicases unwind duplex DNA in catalytic fashion. (els.net)
  • The arrows labelled 'X' indicate the acidic pins used to separate the strands of the DNA duplex. (els.net)
  • It catalyzes the unwinding of duplex DNA in the 3'-5' direction. (ebi.ac.uk)
  • Controlled duplex DNA unwinding is a crucial prerequisite for the expression and maintenance of genomes. (asm.org)
  • Single-turnover unwinding experiments showed distinct lag phases that increased with duplex length because partially unwound DNA intermediate states are highly populated during unwinding. (sciencemag.org)
  • This suggests an unwinding mechanism in which alternating subunits of the dimeric helicase interact directly with duplex DNA. (sciencemag.org)
  • The size and shape of this helicase complex imply that the T-antigen dodecamer complex, assembled at the origin and active in the localized melting of duplex DNA, is subsequently also used to continue DNA unwinding bidirectionally. (asm.org)
  • The central channel of the NurA dimer is too narrow for double-stranded DNA but appears well suited to accommodate one or two strands of an unwound duplex. (lancs.ac.uk)
  • Structural basis for DNA duplex separation by a superfamily-2 helicase. (springer.com)
  • PfPuvB3 is unable to act on blunt-ended duplex DNA, but manifests ATPase activity in the presence of either single- or double-stranded DNA. (beds.ac.uk)
  • There are two broad classes of DNA helicases: hexameric and nonhexameric. (els.net)
  • Models for DNA unwinding catalysed by hexameric DNA helicases. (els.net)
  • A hexameric ring encircles only single‐stranded DNA , which is bound in the central channel to one or two subunits. (els.net)
  • Computer model showing the structure of a hexameric bacterial replicative DNA helicase. (fineartamerica.com)
  • NEW YORK, February 26, 2019 - DNA replication is a complex process in which a helicase ring separates the DNA molecule's two entwined and encoded strands, allowing each to precisely reproduce its missing half. (cuny.edu)
  • Opening of the helicase enables entry of one of the DNA molecule's two strands. (cuny.edu)
  • Damage to both strands of DNA can cause a double-strand break (DSB), as can replication of a damaged DNA template containing a nick. (prolekare.cz)
  • The two DNA strands are also known as polynucleotides as they are composed of simpler monomeric units called nucleotides . (wikipedia.org)
  • The nitrogenous bases of the two separate polynucleotide strands are bound together, according to base pairing rules (A with T and C with G), with hydrogen bonds to make double-stranded DNA. (wikipedia.org)
  • Both strands of double-stranded DNA store the same biological information . (wikipedia.org)
  • The two strands of DNA run in opposite directions to each other and are thus antiparallel . (wikipedia.org)
  • These problems likely arise from the atypical chromatin structure of telomeres, the GC-richness of telomeric DNA that makes it prone to forming DNA secondary structures, and from RNA-DNA hybrids, formed by transcripts of one or both DNA strands. (princeton.edu)
  • Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal (PubMed:25772361). (nih.gov)
  • The arrow indicates the direction of translocation and DNA unwinding. (els.net)
  • For all images, the direction of translocation and DNA unwinding is from right to left. (els.net)
  • Other topics include mediation and modulation of membrane-cytoskeleton interactions, metal binding affinity and selectivity in metalloproteins, translocation and unwinding mechanisms of RNA and DNA helicases , and the structure of eukaryotic RNA polymerases. (thefreedictionary.com)
  • NegC* (similar to NegC in ISWI) acts as an inhibitor to lobe movement and translocation until proper binding of the DNA-binding domain (DBD) occurs. (wikipedia.org)
  • This allows for the activation of the TR domain, resulting in DNA translocation. (wikipedia.org)
  • The DNA stimulated ATPase activity of Dda helicase has been used to probe translocation on single-strand DNA (ssDNA). (elsevier.com)
  • Translocation and unwinding mechanisms of RNA and DNA helicases. (semanticscholar.org)
  • Visualizing ATP-dependent RNA translocation by the NS3 helicase from HCV. (springer.com)
  • The structures reveal a histidine-aspartate (HD)-type nuclease domain fused to superfamily-2 (SF2) helicase domains and a distinct C-terminal domain. (nottingham.ac.uk)
  • Required for DNA resection by the MRE11 - RAD50 - NBN /NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). (rcsb.org)
  • To dissect the role of each activity in DNA repair and infectivity, we altered the AddA and AddB nuclease (NUC) domains and the AddA helicase (HEL) domain by site-directed mutagenesis. (fredhutch.org)
  • the addA(NUC)B(NUC) double mutant lacked detectable nuclease activity but retained helicase activity. (fredhutch.org)
  • Extracts with AddA(HEL)B lacked detectable helicase and nuclease activity. (fredhutch.org)
  • These results support a role for both the AddAB nuclease and helicase in DNA repair and promoting infectivity. (fredhutch.org)
  • DNA-dependent ATPase and ATP-dependent DNA helicase that participates in various functions in genomic stability, including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis. (uniprot.org)
  • The RecQ helicase family is conserved in all organisms and participates in maintaining the genomic integrity of cells. (frontiersin.org)
  • Members of the RecQ helicase subfamily are mutated in several human genomic instability syndromes, such as Bloom, Werner, and Rothmund-Thomson syndromes. (thebiogrid.org)
  • In this study we demonstrate that mutation of Chd2 renders cells susceptible to inefficient DNA repair and genomic instability. (tennessee.edu)
  • The emerging evidence indicates that helicases have vital roles in pathways necessary for the maintenance of genomic stability. (eurekaselect.com)
  • Here, we determined how these activities are affected by the abolition of the ssDNA interaction of the HRDC domain or the deletion of the entire domain in E. coli RecQ helicase. (nature.com)
  • Cas3 possesses a single‐stranded DNA (ssDNA)‐stimulated ATPase activity, which is coupled to unwinding of DNA/DNA and RNA/DNA duplexes. (embopress.org)
  • DnaI binds ATP and exhibits ATPase activity that is not stimulated by ssDNA, because the DNA-binding site on Cd is masked by Nd. (worktribe.com)
  • The ATPase activity resides on the Cd domain and when detached from the Nd domain, it becomes sensitive to stimulation by ssDNA because its cryptic DNA-binding site is exposed. (worktribe.com)
  • In addition, the inherent ability of BLM and RPA to unwind bidirectionally from a nick would facilitate the resection by DNA adduct coated DSB ends which provides ssDNA for nucleases to degrade. (champlainresources.com)
  • Here, we truncated the C-terminal winged-helix-domain (WHD) of Mcm6 to slow down the loading reaction, thereby capturing two loading intermediates prior to DNA insertion in budding yeast. (nih.gov)
  • The horizontal arrows indicate the direction of DNA pumping by the RuvB hexamers onto the surface of RuvA where strand separation occurs. (els.net)
  • In this view, the unwound single strand of DNA can be seen exiting a channel in the RecC subunit. (els.net)
  • Cells in the process of replication have various endogenous DNA damage in the chromosomes resulting from base-mismatch, deletion, DNA double-strand break (DSB) or from replication errors caused by stalled replication forks and other anomalous DNA structures, such as G-quadruplex (G4) DNA. (frontiersin.org)
  • Involved in non-homologous end joining (NHEJ) DNA double strand break repair. (uniprot.org)
  • The mechanisms controlling progression of conjugative DNA processing from a preinitiation stage of specific plasmid strand cleavage at the transfer origin to a stage competent for unwinding the DNA strand destined for transfer remain obscure. (asm.org)
  • In the MOB F family of conjugation systems, the plasmid DNA strand destined for transfer (T strand) is unwound from its complement by a dedicated conjugative helicase, TraI of F-like plasmids or TrwC of the IncW paradigm. (asm.org)
  • BLM plays a role in a number of cellular processes including DNA double-strand break repair, Holliday junction dissolution, and chromosome segregation. (springer.com)
  • To study the role of DmBlm in double-strand break repair, we utilized a genetic assay in which a targeted DNA double-strand gap is created through excision of a P transposable element. (springer.com)
  • This assay can be adapted to test the roles of numerous DNA metabolic factors in DNA double-strand gap repair. (springer.com)
  • Once activated, the helicase runs the length of the strand, separating them and initiating the replication process. (cuny.edu)
  • The contributions of the Sgs1, Mph1, and Srs2 DNA helicases during mitotic double-strand break (DSB) repair in yeast were investigated using a gap-repair assay. (prolekare.cz)
  • Post-DNA binding, the DBD causes tension on the DNA strand resulting in a conformational change that blocks regular NegC* inhibition. (wikipedia.org)
  • This structural alignment suggests a mecha- nism for 3′ to 5′ nucleolytic processing of the displaced strand of invader DNA that is coordinated with ATP-dependent 3′ to 5′ trans- location of Cas3 along DNA. (nottingham.ac.uk)
  • DNA helicases Sgs1 and BLM promote DNA double-strand break resection. (semanticscholar.org)
  • Sources of DNA double-strand breaks and models of recombinational DNA repair. (semanticscholar.org)
  • This review is focused on helicase pathways, with a special emphasis on DNA cross-link repair and double strand break repair, that impact cancer biology and how cancer cells may be chemosensitized through the impairment of helicase function. (eurekaselect.com)
  • Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. (specklab.com)
  • For example, ROS induces oxidative base modifications, IR typically leads to single- and double-strand breaks (SSB and DSB, respectively), DNA alkylation can lead to adduct and interstrand crosslink (ICL) formation, and UV triggers the formation of thymidine dimers ( Hurley, 2002 ). (frontiersin.org)
  • Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:26300262). (rcsb.org)
  • Also promotes cell death and DNA double-strand breakage in response to replication stress: together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress (PubMed:23319600, PubMed:23361013). (nih.gov)
  • DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. (nih.gov)
  • The Saccharomyces cerevisiae yeast Srs2 helicase has a major role in one of these pathways. (asm.org)
  • RecQL4-dependent association of Mcm10 and Ctf4 with replication origins appears to be the first important step controlled by S phase promoting kinases and checkpoint pathways for the initiation of DNA replication in human cells. (nih.gov)
  • Proliferating cells have a different repertoire of DNA repair pathways than quiescent cells and, therefore, the same lesion might have different effects in different tissues. (frontiersin.org)
  • pJK2041(mini-F-Km r - recQ + ) was constructed by ligating the Bam HI- Eco RI recQ + DNA fragment with the Bam HI- Eco RI fragment of a mini-F vector, pJK289 ( 17 ). (pnas.org)
  • The BLM helicase is a member of the RecQ DNA helicase family and is mutated in the cancer-prone disorder Bloom syndrome. (springer.com)
  • The generic and versatile nature of the assay makes it applicable to a variety of DNA helicases and DNA templates. (nih.gov)
  • In this study, we characterized the DBD of human papillomavirus 11 (HPV11), HPV18, and BPV E1 using a quantitative DNA binding assay based on fluorescence anisotropy. (asm.org)
  • However, having a single qPCR assay (the DNA helicase standard curve) for RLOv detection/quantification might be sufficient, rendering a second qPCR assay unneeded. (onsnetwork.org)
  • The human F-Box DNA helicase FBH1 faces Saccharomyces cerevisiae Srs2 and postreplication repair pathway roles. (nih.gov)
  • These results suggest that ATM and BLM have largely distinct roles in recognizing different forms of damage in DNA, but also compatible with partially overlapping functions in recognizing breaks in radiation-damaged DNA. (nii.ac.jp)
  • Helicases play key roles in a wide range of cellular activities involving DNA and RNA transactions, making them attractive anti-malarial drug targets. (beds.ac.uk)
  • RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. (cusabio.com)
  • A Zn2+-binding module mediates the interaction with the helicase and C67, C70 and H84 are involved in the coordination of the Zn2+. (worktribe.com)
  • In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBXO18) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location (PubMed:25585578). (nih.gov)
  • Crystal structures of several uvrD-like DNA helicases have been solved [ PMID: 9288744 , PMID: 10199404 , PMID: 15538360 ]. (ebi.ac.uk)
  • Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP. (ebi.ac.uk)
  • Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism. (ebi.ac.uk)
  • We propose that binding of DNA flap structures by excess Mph1 may block Rad27 action, creating a mutator phenotype that phenocopies rad27 Δ. (genetics.org)
  • c ) Crystal structures for apo UvrD (3LFU) and UvrD-DNA complex (2IS2, the closed state). (elifesciences.org)
  • Within eukaryotic cells, DNA is organized into long structures called chromosomes . (wikipedia.org)
  • Here, an optical method has been developed to follow the unwinding of multiple DNA molecules simultaneously in real time. (nih.gov)
  • The E1-E2-DNA complex is comprised of an E2 dimer and two molecules of E1 ( 11 ) and serves as a starting point for the assembly of larger oligomeric E1 complexes, either hexamers or double hexamers, that have unwinding activity ( 20 , 47 ). (asm.org)
  • DiNardo S, Voelkel K, Sternglanz R. DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication. (pubmedcentralcanada.ca)
  • To investigate the function of these two molecules in DNA damage, recognition, we generated double knockouts of ATM-/-BLM-/-In the DT40 chicken B-lymphocyte cell line. (nii.ac.jp)
  • DnaI is sufficient to load the replicative helicase from a complex with six DnaI molecules, so there is no requirement for a dual helicase loader system. (worktribe.com)
  • It is widely appreciated that the ends of linear DNA molecules cannot be fully replicated by the conventional replication apparatus. (princeton.edu)
  • The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. (asm.org)
  • Supercoiling of intracellular DNA can occur in eukaryotic cells. (pubmedcentralcanada.ca)
  • Eukaryotic organisms ( animals , plants , fungi and protists ) store most of their DNA inside the cell nucleus as nuclear DNA , and some in the mitochondria as mitochondrial DNA or in chloroplasts as chloroplast DNA . (wikipedia.org)
  • Difference between eukaryotic DNA replication and bacterial replication? (answers.com)
  • CRISPR-Cas3 destroys the targeted DNA in either prokaryotic or eukaryotic cells. (wikipedia.org)
  • Human telomere length regulator Rtel1 is a superfamily II DNA helicase and is essential for maintaining proper length of telomeres in chromosomes. (hindawi.com)
  • All remodelers fall under the umbrella of RNA/DNA helicase superfamily 2. (wikipedia.org)
  • Cas3 contains N‐terminal HD phosphohydrolase and C‐terminal Superfamily 2 (SF2) helicase domains. (embopress.org)
  • UvrD-like DNA helicases belong to SF1, but they differ from classical SF1/SF2 by a large insertion in each domain. (ebi.ac.uk)
  • UvrD-like DNA helicases unwind DNA with a 3'-5' polarity [ PMID: 10679457 ]. (ebi.ac.uk)
  • Bacterial UvrD helicase. (ebi.ac.uk)
  • Functional switching of UvrD helicase. (elifesciences.org)
  • a ) Schematic illustration of unwinding by a DNA helicase (UvrD). (elifesciences.org)
  • The model that these data allow us to propose helps to reconcile different interpretations of RecQ family helicase function that have arisen between work based on the S. pombe system and models based on studies of Saccharomyces cerevisiae SGS1 suggesting that RecQ helicases act before Rad51. (thebiogrid.org)
  • The interaction of the Sgs1 helicase homolog and the Top3 topoisomerase is reminiscent of the recently described structure of reverse gyrase from Sulfolobus acidocaldarius, in which a type I DNA topoisomerase and a helicase-like domain are fused in a single polypeptide. (asm.org)
  • hDNA analyses suggest that all three helicases promote SDSA, and that Sgs1 and Srs2 additionally dismantle HJ-containing intermediates. (prolekare.cz)
  • The mechanisms supporting localized unwinding within the complex include initiator-induced DNA looping, wrapping, and bending and feature regions of low thermodynamic stability. (asm.org)
  • Two Pif1 Family DNA Helicases Cooperate in Centromere Replication and Segregation in Saccharomyces cerevisiae. (princeton.edu)
  • Getting it done at the ends: Pif1 family DNA helicases and telomeres. (princeton.edu)
  • Below are the list of possible Replicative DNA helicase products. (mybiosource.com)
  • Pif1 family helicases are found in virtually all eukaryotes. (princeton.edu)
  • Saccharomyces cerevisiae (Sc) encodes two Pif1 family helicases, ScPif1 and Rrm3 ScPif1 is multifunctional, required not only for maintenance of mitochondrial DNA but also for multiple distinct nuclear functions. (princeton.edu)
  • Belongs to the DEAD box helicase family. (abcam.com)
  • This review focuses on one such class of DNA helicases, the Pif1 family of 5'-3' DNA helicases. (princeton.edu)
  • This approach has led to the identification of RECQL5, a member of RECQL-helicases family, as a new BC susceptibility candidate, which deserves further study. (cdc.gov)
  • Differences in properties of RuvB between the malaria parasite obtained from the study and human host provide an avenue leading to the development of novel drugs targeting specifically the malaria form of RuvB family of DNA helicases. (beds.ac.uk)
  • Similar to the imitation switch (ISWI) subfamily of ATP-dependent chromatin remodelers, CHD complexes regulate the assembly and organization of mature nucleosomes along the DNA. (wikipedia.org)
  • Christian Speck gives a talk on the key steps in the loading and activation of the replicative helicase MCM2-7 at the Wellcome Trust meeting on Chromatin: From nucleosomes to chromosomes. (specklab.com)
  • What is the difference between DNA and chromatin? (answers.com)
  • Here the authors show that SUMO2-conjugated PCNA destabilizes RNAPII from chromatin, enhances replication progression and limits transcription-induced DNA damage at common fragile sites. (nature.com)
  • Overview of all the structural information available in the PDB for UniProt: Q9H611 (ATP-dependent DNA helicase PIF1) at the PDBe-KB. (wikipedia.org)
  • The toxicity of DNA damage depends on the structural changes they inflict as well as the characteristics of the cell they occur in. (frontiersin.org)
  • Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. (specklab.com)
  • It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction. (ebi.ac.uk)
  • During late M-phase of the cell-cycle, Cdc6 binds to ORC and the ORC-Cdc6 complex loads in a multistep reaction and, with the help of Cdt1, the core Mcm2-7 helicase onto DNA. (nih.gov)
  • We found that the HPV11 DBD binds DNA with an affinity and sequence requirement comparable to those of the analogous domain of BPV but that the HPV18 DBD has a higher affinity for nonspecific DNA. (asm.org)
  • In contrast, the HPV11 E1 helicase purified as preformed hexamers binds DNA with little sequence specificity, similarly to a dimerization-defective DBD. (asm.org)
  • Chd2 mutant cells show inefficiency in DNA repair of DNA lesions induced by X-rays and UV irradiation as assessed by single cell gel electrophoresis assays. (tennessee.edu)

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